Document Yj5bjNKek1yvn3BM7exV90JvE

ARR26 _ 0L63 ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TESTSUBSTA0N 000C 0000E 00 Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-85, or as a component of FC-803F. (1Octanesulfonic acid) (CAS # 2795-393). Remarks: The 3M production lot number was 501. The test sample is FC- 603F. Current information indicates it is a mixture of 0.5% PFOS, 7% diethylene glycol butyl ether, 82.95% water, 2% acrylic foamer, 4% Alkylether sulfate, 1.45% Xanthan gum, 1.4% calcium acetate, 0.07% N-(3- Chloroallyl)hexaminium chloride, 0.5% triethanolamine, 0.05% sodium hydroxide, 0.03% cis-1-(3-Chloroallyl)-3,5,7-triaza-1-azoniaadamantane chloride, and 0.05% tolytriazole. ~ The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect the toxicity of the fluorochemical component of thetest sample. METHOD: Method: OECD Guideline 203. Type: Acute static GLP: No Year completed: 1994 Species: Pimephales promelas Supplier: Aquatic Biosystems, Inc., Fort Collins, CO. Analytical monitoring: Temperature, pH, and dissolved oxygen. Exposure period: 96-hours `Statistical methods: LCs values were calculated by the Trimmed `Spearman-Karber method. Test fish age: Juveniles (53 days) Length and weight: Average length = 16 mm Average weight = 0.0276 g (wet) Loading: 0.276 g/L. Pretreatment: None. Test conditions: Dilution water: Carbon-filtered well water (3M Well #2, St. Paul, MN) Dilution water chemistry: Not given. Lighting: Not given. Stock and test solution preparation: The test material was added directly ~~ to the dilution water on a weight/volume basis. Concentrations dosing rate: Once. Stabilityofthe test chemical solutions: Not noted. Exposure vessels: 1L vessels containing 1 L test solution. Number of replicates: 2. 002447 Number of fish per replicate: 10. Number of concentrations: five plus a blank control Water chemistry during the study: pH ran8g.e0(80-.936 h(coounrtsr)ol: exposure) 8.08.3 (560 mg/L exposure)* Temperature range (0-96 hours): 19.5-20.5C Dissol6ve.d88ox.y5gmegnlrLa(ncgonetr(o0l-9e6xphoosuurrse)): 3.6 -- 8.5 mg/L (560 mg/L exposure) *be5c6a0usmeg/thLe(hsiegchoensdt hciognhceesnttrcaotnicoenntursaetdiorne)suelxtpeodsiunrteotvaallmuoerstaulsiteydby 24 hours. RESULTS Nominal concentrations: Blank control, 100, 180, 320, 560, and 1000 mg/L. Element values: 96-hour LCso = 750 mg/L (CI not calculable) Element values based on nominal concentrations RSuebmsatraknsc:eTReestmianrgkwsafsielcdo. nTduhcetveadlouensarmeipxotrutreedaapspldyetscoritbhaetd miintxthuereTeasntd not the fluorochemical proportion alone. CONCLUSIONS `The FC-603F 96-hour LCso for fathead minnow was determined to be 750 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kiimisch ranking = 2. This studymeetsthecriteriafor quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmationof the amount of fluorochemical proportion in the solution. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number M1655, 1994. OTHER 002448 Last changed: 6/26/00 002449 BEST COPY AVAILABLE ENVIRONMENTALLBORATORY Ouitn SIs2Fs15T Arata 85 Pimephaios promeies | 96-hour Toxicity Test Data Summary SAMPLE: FC-603F (Lot #501) Sarpl(moarGtyor|26hours | 48hPoourrcso|n Mo7ra2ihtoyurs | 98hours 511208000 ooo oo0 ooo 00o stsooo | 1o00 1o00 1o00 1o90 Lest Estimation Method: Trimmed Spearman-Karbor 2f7a24nh01LL0oc5a300 (0(s55e6%%GCGIllyyy ==. + 775500 750 mmoLt mL ((Naott (Nat CGGallleeolbsbel))e ; 58h L050 (03% Cy = 750 mo. 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