Document YXxJYNvodXmv3Ydj9kzvoLbD
AR226-0384
ACUTE TOXICITY TO AQUATIC INVERTEBRATES (E.G.,DAPHNIA) TEST SUBSTANCE
Identity:
Perfluorodecanesulfonate, ammonium salt; may also be referred to as PFDS, FC-120, or 3M Sample J2905-1. (1-Decanesulfonic acid, 1,1,2.2,3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10heneicosafluoro-, ammonium salt, CAS # 67906-42-7)
Remarks: Test sample is a mixture of the test substance in water and butyl cellosolve (approximately 25% test substance, 37.5% butyl cellosolve and 37.5% water). All values reported relate to this mixture. No calculations were made to adjust for the actual concentration of the test substance in the test sample. Test sample was from 3M Lot 164.
METHOD
Method: OECD 202.
Test type: Static acute
GLP: No Year Completed: 1992
Species: Daphnia magna
Analytical monitoring: pH, dissolved oxygen, conductivity, temperature. Test substance concentration was not monitored.
Statistical methods: EC values calculated using Binomial/nonlinear interpolation
Test daphnid source: In-house cultures, EnvironSystems Division,
Resource Analysts, Incorporated, Hampton, NH
Test daphnid age at study initiation: < 24-hours
Test conditions
Dilution water: Aerated, filtered natural well water
Dilution water chemistry (from a representative sample):
hardness:
186 mg/L as CaCO;
pH:
72
Conductivity:
540 pmhos/cm
Stock and test solutions preparation: A primary stock solution was prepared in dilution water to yield a test sample concentration of 1000 mg/L. All test solutions were made by diluting the appropriate amount of stock solution with dilution water. Exposure vessels: 250 mL glass beakers containing 200 mL of test solution.
Number of replicates: 4
Number of daphnids per replicate: 5
Number of concentrations: five plus a negative control
000136
Water chemistry during the study:
Dissolved oxygen range (0-48 hours):
8.7 --9.3 mg/L (control exposure)
PH ran8g.e8(-094.81mhgou/rLs):(100 mg/L exposure)
7.2-8.1 (control exposure)
7.2-8.2 (100 mg/L exposure) Test temperature range (0 -- 48 hours)
19.4 -20.8C Element basis: mortality and immobilization
RESULTS Nominal concentrations: BK control, 15, 25, 40, 60, 100 mg/L. Element value: 2448--hhoouurr EECCsso ==4>410(025mg--/L6.0) mg/L cStaaltciusltaitceadluEsvianlguatthieobni:noTmhiealEinCosnlivnaelaureianntedrp9o5la%ticoonnmfeitdheondc.e interval was RMoermtaarliktsy:oVfaclounetsrorlesp:orNtoendeare for the test sample (mixture). No scaulbcsutlaanticoensinwtehreetemsatdseamtpolaed.just for the concentrationof the test Cumulative percent mortality and immobilization:
Tos"Cnootne.o| 2e 6s [oe
hosc0o|na0l|
[o[w w|
Low [|= w |
cCoOnNcClLuUsSIiOoNnSs The test sample 48-hour ECso for Daphnia magna was determined to be
44 mg/L with a 95% confidence interval of 25 to 60mg/L.
000137
Tweastteirn(g~w2a5s%csounbdjueccttemdatoerniatlh)e. "aTsh-esovlad"lumeisxtruerpeorotfetdhaepspulbyjetcotthmaattemriixatlurine
and not directly to the subject material.
SStu.bmPaiutlt,erM:inn3esMotCao,m5p5a1n3y3, Environmental Laboratory, P.O. Box 33331, PDAATARQQUALITY. aRnealliyatbiiclailtyd:atKaiifomri:sdcehtrearnmkiinnagti2o.noTfhtihsesttuedsty,suwhbislteanweclelccoonncdeuncttreatdi,olnaicnksthe test solutions; and determinationofthe sample purity. Because a mixture winatshetemsitxetd,urcearcoeumludshtabvee teankheanncwehdenorinrteesrtprricetteidngbitohleogreiscualltsu.ptTakheeasnoldvoernt
toxicityofthe fluorochemical component.
Be EREFEERREENNCCEESS.erie TIhnicosrsptoruadtyewd,asHacmopntdounc,teNdebwy HEanmviprsohSiyrsetoenmsbeDhiavlisfoiofnthRees3oMurCcoemApnaalnyys.ts, oOmTHeERr
Last changed: 5/18/00
000138
_-- Ja9cy
Study Title SttaotitcheacDuatpehaTiodx,icDiatpyhnoifa Fmca-g1n2a0
authocs TRoibmeortthy 1.3. Bvoaerria Study completed revruacy, 1992
PerformingLaboratory
ResouErncveicAonsaylaytsetses, DIinvciosrpioorated Hampto`no,ne NLeawfaNyaemtpteeniRsoed 03842
Page 1 of 10
000139
~
I. sumere
descriTbheed aciuntethitsoxifciintayl roepfortF.C-1T2h0e tteostthewasdacpohniddu,ctDeadphfnoira3mMagCnoam,paniys Dfiovrisi4o8n hooufrsRedsuoruirncge AnPaelbyrsutasr,y In5c. toin H7,ampt1o9n9,2, NewatHatmhpeshEinrvei.roSyIsttewmass cBooenrdiu,cteandd TbyimoJtehyannWaerd.Magazu, Peter Kowalski, Ellen Stanford, Robert
conceTnhteratiotnesst ofwastestpersfuobrsvteadnceundaenrd
astadtiicluticoonndiwtaitoenrsconwtirtohl
five at a
wtaetmepreratcuorlelectoefd 20at: H1aCm.ptonT,heNdeiwluHatmiposnhiwraet.er wAaesratfiiolntewraesd nnoattureamlplowyeeldl
Ntoominmaalintacionncendtirsastoilovnesd ooxfygenFC-c1o2n0cenwterraet:ionOsmga/boLve(caonntaroclc)e,pta1b5,le25l,eve4l0.,
c0a,lculaatnidons.100 mg/L. Nominal concentrations were used for all
and wDearpehnidlsessusetdhanin24thehoutresstolwdereat rtohdeucsetdartfroofm tahneitne-shto.useAfctuelrtur4e8
hours of (blotted
exposure dry) of
the control daphnids 0.0002 g, resulting in
ahaldoadianng
aravteeragoef
w0e.t00w5eigg/Lh.t
ALL daphnids were in good condition at the beginning of the study.
ECS0 Eoxfposu44re mgOf/L daFpC-h1n2i0d,s twoiththeates9t5%sucbosntfaindceencreesulitnetdervianlaof4825houtro 6th0e mgs/uLr.vivingSixdaofphntihedssuerxvpiovsiendg datophni1d0s0 emxgp/oLsedwetroe60immmgo/bLilainzdedalalt o4f8 hours. No other sublethal effects were observed in any test vessel.
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II. TABLE oF CONTENTS
section:
ace
I. summary
2
II. Table of Contents
3
III. Index of Tables and Figures
4
I. Introduction
5
V. Methods and Materials
5
VI. Results
8
VII. References
n
Appendix A. Water Quality Data from Toxicity Test
12
Page 3 of 1
000141
;
1. nom or mates wo ricuzs
Tablo 1. $C3h5e7m1i0calofchnaactaurcatlerivaealtioanteorf uarerd.eparsesdeiniteattiiovne
sacs .
Catee tor toxicity cast
THBLo 2. Survival and sublethal effect daca trom
toxicity test
bg
TAB10 3. Nferdolnantoexfifceicttyivreastconcentrations (reson)
x
|
TAbLo A.1. SCwoo1n9du0cticvointcya,ntepai,tiosnesmpeecsamtuurceed,dearnidngditsosxoilevieedy 1
' |
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000142
w. nemmooverion dtaobhjneeccrtidibavepTehh.niistdho,ef satmutedhftyerheosdwhssawtsautdaenyrdspmwioaannstvseeorrrtiteoaedlbdsrbeaytteeemrI.pmMlionCyoehmeepdtahnesiyne,cptouhrStette. csortPneaetduxaylii,,cnis'MatniydnsneoetcfshtoeiFtoCanri.sezmoeciThehrisee tOfhe vtahteoriqnuvaelsittiygatdiaotna.coTlhleactreapdordturianlgasthceonttaosien.s an appendi that presence
est sussTnce:
Vv. wemioos ao uaTeRIRLS
LBEoOntLvEilrso1s64yO,stth1aetm02s-vBaustoon(rlEyanNbvoeiEvlctelohmesabdynesorwtlie.tm1"hs3, thF1eCS9-9a11mf.2po0lleluoIevwsiunwmagsbsuepirpnclfooinret1mda0ai3tnbi8eyo)dno:HinwCa~os43u2p02a05dn40ey-l,1ai,lve93rFp5erCdaaBssut0sit,hco
EfAvhneevniuceod,sayraktSet.mastPafuorlro,omaMmiintnneeimsmpouetmraa.tofure1P.0rioyrearAtso. ruesseertvhae Lessatmplmeatweirlile:bewesrevsetioereeds ainn
i
orLuTion wazens
!
I
Water used for acclimation of test organisms and for all toxicity
i; |
aaHtearernesapttaipehnrdige.rsee.ntweaRdseWaaiuntcletoTnrlalonweflacestecdh1s.etmoirfceradolmiannwae5ll0yl0ss-igsalaoltfonaEpnorvleiypreroteShsyyeslntetenamestivteainnkssaHmaspmlhpeotrooenf,istavuNeeersw
i
TST oRoAnTH:
!i i
ussoeudzceinJauntvdheeniltveeernetdwaepirhdeneindptsriofideuedcmepdulsoiyfnergdonanaansapipntr-eoshptoeuisseotrecgualnttiausxrmoesnomawinecdrewkeefrrr.eomLDaeaspsshiancighdlaene
!
i
ojt2aah4riesn.thacoiounrnescdlDuusroiilionndng 1a00aot1fcctldhiietmlheaustttiiaootrnntoxidaofcatipethtrynhiedusnttdeewssetter..restCanootrtniitcortrocrloenatddtoieadtptihaonsfntoisisdnsgdi,iwnsee4rdaeesLpewiheicaidengedhegdltwahoserasyet
Dueergeinnifnrgee the test
ofof thaeppatreesntt. initiation the
frout chow and/or the
tseimcDpkuenrreisanstg,urteheivnaajscucril2e0is.m,3a:tCi.oanndbpaeprshibaonidodrss24avleihcroieseessfedpartyieoasnttn.o freshwater alge Selensstrus cepsicormstum once
ox tuice daily before the test.
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000143
FE 4
Table 1. nCahteumriaclalwelclhawraatcetreruiszeadtioans dioflutiaonrweaptreersenftoratitvoexicistaymptleestof
--_-- Paraneter _--
oH
Unie of Heasurenent oH unite
Reporting Linic
-
Measured value 7.2
Conductivity
unhos /cm
-
ss0
Hardness
g/L as caco, -
166
organochlorine
warn
pesticides
0.5
wo
Organophosphorus ug/L
2
pesticides
wo
Pboilpyhcehnlyolreinated
s/n
1
wo
Notes: 21.. NpiD,= nhaortdndeestse,cteadndatconodrucatbiovvietythweerreepmoeratsiunrgedlimiint.dilution
wiantterrodcuocltlieocnteodf ofrrgoamnitshemscaonndtroplesttiesctidevesasnedlPCpBridoartatois
"
collected during routine biannual water quality testing.
Page 6 of 13
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|
ToxzeITY TESTING:
JanuaryA 21screteoni23n,g 1t99e2s.t Nwiotmhinalthecotnecsetntrsautbisotnasncoefwatsestconsduubcstteadncdeurwienrge
s1u,r1vi0v,al 10a0t, 100anda'nd 11,0,00000mgn/gL/.L aAnfdte80r%48surhvoiuvrasloofrexgproesautreer attheraellwalsoweOrv
tested January
c2o9ncetnotra3t1,ion1s9.92.
NAomdienfailnitciovnecenttorxaitciiotnystoefsttowsats sautbtsetmapntceed wferroem
l1e50s,s th2a50n, SOV400s,urvi6v0a0,l ocacndurre1d,0a0t0 amgl/lL.tesTthediscotnecstentwraastiroenpse.ated because
7, 1992T.he deTfhienittiesvte watsoxiccointdyuctteedstatwasa tpaerrgfeotrmetdemdpuerraitnugreFebofrua2r0y 51tCo cwointthrolf.ive Aconc1e,n0t0r0atmigo/nLsstoofck steosltutiosnubwsatsanmcaedeabnyd coambidniilnugtio0n.25wagteorf athdejusttiensgt tshuebstafnicmealandvolduirleutiotno wa2t5e0rmlisn wait2h50dmillutvioolnumewtatreirc. flNaosmkinaanld c6o0,nceanntdra1t0i0onmsg/L.of the test material were: O mg/L (control), 15, 25, 40,
of eacThwetnrteaytmednatp.hnidTshe wteesrte waesquaplelryfordniesdtriibnut2e5d0 malmongglasfsoubrearkeeprlsicattheast
Tceosnttainveedssel2s00 mwlereofrtaensdtomlsyolaurtriaonnge(dwatienradnepitnhcuwbaastorapdpurroixnimgattehley486 hcmo)u.r
test (a vessel).
rAand16omhounrumbleirgsht atnadble8 hwaosurudsaedrktpohosteolepcetriotdhewalsocaauttioonmatoifcaelalcyh
maintained with cool-ghite fluorescent lights that provided a light
diinstseonlsvietdy oofxyge2n0 couEnscelnmtr2a.tionsAearbaotvieonaccweapstablneot levreelqsu.iredDapthonimdasinwtoarine
not fed during the test.
immobiliTzhaetionnumb(eirnabiolfitysurtovivisnwgim orwgitahniinsms15andsecotnhdes ocafctuerrrencgeentloef asguiitmantiingo,n) loosrs otofherrefsluebxl,ethaelxciteafbfielcittsy, (ldosisscoofloreaqtuiiolni,briourm,chaenrgraetiicn abenhdavi4o8r)hourwse.re Didsestoelrvmiendedoxyvgiesnual(lYySIaMnodderleco57rdemdeteirn)i,tiaplHly(Baencdkmaanftmeorde2l4 tPHeImpera1t2uremet(eArS)T,M mecrocnudruycttihveirmtoymet(eCro)lewerPearmmeerasurneudmbearnd r1e4c8o1-r6d0e)d,daialnyd in each test chamber that contained live animale.
STATISTICAL METHODS:
statistiRceaslultstechonfiqutehse
(tSotxepihcaint,y
test 1983).
were The
24inatnedrpr4e8tehdourbyECSOstavnalduaersd
cwaelrceulactailocnuslatweedreby tpehrefobrinmoemdiably,thmeovianugthoarveruasgien,g onromipnraolbitcomnectehnotdr.atioAnLsL
of the test substance.
Page 7 of 1
000145
vi. mesurs
throughoAuLtL ttehset vteessts.els coBniotlaoigniicnaglFaCn-d12w0atweerrequcalleairtyanddataremgaeinneerdatecdleabry
rtehsepecatciuvteely.toxiciOtnye hteusntdredareperpcreenstentesdurviivnalToacbcluerre2danidn tAhpepencdoinxtroAl,
exposure. 0.0002 g
at
Control the end
dofaphthneidsteshta.d
anLoaavdeirnaggerawteetduwreiingghtth(ebltootxtiecditdryy)tesotf
was approximately 0.005 g/L.
ECS0 ofExpo44suremg/Lof dFaCp-1h2n0i,ds twoiththeates95t%sucbsotnafnicdeencreesulitnetdervianlaof4825houtro 6t0he ngs/uLr.vivingSixdaofphntihedssuerxvpiovsiendg datpohni1d0s0 exmpgo/sLedwteore60immmgo/bLiliazneddalalt o4f8 dhuoruirsn.g theNotesto.ther sublethal effects were observed in any test vessel
Page 8 of 13
000146
Table 2. Survival data from toxicity test
--_--_--mn
conNcoemnitnraaltion
_--Number Alive
Number Affected
--T _--_--(09/1) rep. Onc 20hT 48 hr oh: 2anc ashe
(control) 1 2
ss ss s5
o
.3
ss 55 ss
oo oo
15
21
s5
5s
s :
o
o
s3
ss s4 s3
o 3o
25
1 2
s s
s s
3 s
o
:3
5s `s 35
o
o o
I
21
5s
`s
s 3
0
0
s3
ss s5 2
o o o
60
1 2
s s
s 5
12
o 11
e3
ss 5s 23
oo o 22
100
21
ss s3
o o
3 s
5 s
35
11
o o 11
--_------
Note: sAefcfoencdtsedatotregrangiesnmtwleereagiitmamtoiboinl)i.zed (unable to swim within 15
ERYEY
000147
Table 3. Ex_po--sure p_er--tod 20 hours 48 hours
_--_--
Median effective concentrations (ECSO8) from toxicity test
cso
5100 mast 44 mg/L
cont95idepnecrceen1tiits .
25 - 60 mg/L
e Calmceutle haotdion -
BinoImnitaelr/pNoolnaltiinoenar
|
|
Page 10 of 14
|
4'
000148
vin. RersmeNces OECD. aBnido1t9Ri8ce4.proSdGyusuctietdmiesol.nineTesasttfh.oordATde2o0s2pt,tiendDgaApophfrniiCldhem4s.ipc.a1l9s6A.4c.uteSecItmimoonbil2:izEaftfieocntsTeosn Stephan,U.SC..2.EPA1.383D.uluCtohm,puKtNe.r PPerrosgornaanlfCoormmtuhneicCaatlicounl.ation of 150 Values.
4
Page 11 of 14
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Aopendix A. WATER QUALITY DATA FROM TOXICITY TEST
Page 12 of 14 .
000150
Table AL. cCoonndcuacnttievaictiyo,n mpie,amstzeemdpersautruirneg, toannidcidtiyssoolmveed oxygen
-- wosinai S [ryeb---- er--n 7]
c--o_nc--Cenatyratm ion Ree h hoe hwow nwe e No ee h2 e owne
Otomo D3 1 os0seeo Ssseoe omwsoee Gs se se
122h23 eemnno asaeo 23 en oe
1s
3 Iossoso smswoo ossemoee oso sm ame
27735a az
aaennn aas8eo en ae
2s
D 3m sosm ssseeo ssomseeo EEE
2a73h2 aoonhh saaloea
w
ES SH dLoosoes sosoe sseo 21h2 eens oaha
o3mse ssee aosee aanz esn doa
D 3 1s s s so
sssewow smsoeee so cme
aa222 23
oaeF3z 53
aaognd ed
100
D3 Iossso EOE
sssmwoo smseeo SR
77735a or
eeeahz eeanng HCH
eee
Page 13 of 14
000151
BO
_ mom oo
oce152