Document YVNVvpEMeQq0gEDemw9Ld9eE
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AR236-150
STUDYTITLE ExtractionoYfoPloktafosrsAinuamlyPseirsflUusoironogcHiaPnLeCs-uEllfoencartoesfprroamy/EMgagssMeSmpbercatrnoem,etArlybumen, and
DRAETQAUIREMENTS Analytical Method Requirements
STUDY DIRECTOR
Sean Gallagher
STUDYCOMPLETEDON
:
September 06, 2001
SE
EERFORMINGLABORATORY/TESTING FACILITY Centre Analy3t0i4ca8lRLeasbeoarartcohriDersi,veInc. (Centre) - StPahtoenCeo:ll8e1g4e-,2P31A-810638201
STUDYSPONSOR 3M EnvironmentaBluiTledcihnngo2l3ogEy-0a9nd Safety Services
St.PauPlO,MBoNx 5353133331-3331
EROJECT CentCreentPrreotSotcuodlyNNuummbbeerr::0002P3--002633-063
"Total Pages: 102
000243
Con SyNo:23.063
FPLCBlciorprayMssSpactromety"condscied fo SH Evista Tochaonny Cento Study
Troon
Number 023063, eotitkd `Bxinction tn B03 Meximne Abner, su Yok
of Potassium Go Ansys Ving
4ndSafetyServices, wasperformedincompliancewithUSEPATSCAGoodLaboratory Practice Standards (40 CFRPart 792) by Centre Analytical Laboratories, Incwith the followingexceptions.
1. $i7c92e.6f3o.rMheabieandbtessaannideeCalni0bectsteieocnraocf eo hoof Equipment.NoequipmentSOP ha in
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000244
_--_--mmm--------,----e CentreStudyNo:023-063
M0`C2ee3mn-bt0rr6ea3nA,nea,leynttiAictllabeldu,mLaebn"o,Erxattroaarncidteiso'nYQoulaoklfitPyfooArtsassuAsrniaaunlmycseiPsUenrifltUusroirenovgoicetwaHenPedLsCuCel-fnEotlnreaetcetS.truodsfyprroNamuymMbEaegssrg apASpnplaeilcycttraiobcmlaeeltGrLoya"ob.odrLaAatlbolroireeasxt'aomrSiytnPaerndadcaptrhidacseOSeptsearwnadetarirendgsr.ePvrioAeclwelfediudnredfsio,rngctsohwneedruScettruedapycocrPotrreodtdionctogolt,thoeaCSntedundtayrlel Diranedtocmantageomenrt.
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`CeatreAnalyticalLaboratories, nc.
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ComStyNe: 30 CERTIFIOFCAUATHTENTIICOITNY
D Smita v n Ge CJFom onrt eAenstamonLv, Thisreport, forCentreStadyNumber023-063,is trusandcomplaterepresentationof
PrincipalInvestigator, Centre:
Luton Emi Scientist
Con Any abortion FstMtge a ABbaidd4. gos~
CentreAnalyticalLaboratories, Inc.
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StudyDirector
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Soxn Gallagher `WildlifeInternational, Lid.
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000246
Centre Study No.: 023-063
STUDYIDENTIFICATION
"Extraction`oYoflPkoftoarsAsniaulmysPiesrfUlsuionrgooHcPtLaCn-eEsullefcotnraotsepfrraoym/MEagsgsMSepemcbtrraonmeet,rAy,l"bumen, and CENTRE PROTOCOL NUMBER: 00P-023-063
CENTRESTUDY NUMBER: 023-063
TYPEOFSTUDY:
Analytical
SAMPLE MATRIX:
MaYlollkardandQuailEggMembrane, Albumen, and
`TEST SUBSTANCE:
Perfluorooctanesulfonate (PFOS)
SPONSOR:
3BMuiElndvinigro2n-m3e8n-0t9al Technoanld Soafgetyy Services PO Box 33331 St. Paul, MN 55133-3331
STUDY DIRECTOR:
WSieladnliGfaellIantgehrenrational, Ltd. E8a5s9t8oCn,omMmDer2c1e6D0r1ive
`TESTING FACILITY:
3C0en4t8reReAsneaalryctihcDarlivLea:boratories, Inc. State College, PA 16301
TIAMNEATLYATBILCEA:L PHASE
`ESxtpuedryiImneinttiaatlioSntaDratteD:ate: `ESxtpuedryiCmoemnptlaletTieornmiDnaatte:ion Date:
03/26/01 0326/01 04/02/01 09/06/01
`Centre AnalyticalLaboratories,Inc.
Page Sof 102
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CentreStudy No: 023.063
PROJECTPERSONNEL T`Thhee fSotluldoywiDnigrepcetrosronfnoerltfhirsomprCoejnetctrewAansalSyetiacnalGaLlabloargahteorriaets,WiIlndc.l,ifweerIentaersnsaotciioantaeld, wLiitdh. `various phasesofthe study:
Name EmilyDecker Dave Bell Mitra Arjmand Ling Ling Lin Yu Qiong Tan XisomingZhu Lawrence Ord Rickey Keller `Shawn Robb.
Title Scientist Scientist Technician Technician Technician Technician Sample Custodian `Sample Custodian `Sample Custodian
`Centre Analytical Laboratories, Ine.
Page 6of 102 000248
Cente Study No. 023-063
TAOFB COL NTEE NTS
TQGIUOLAOBLDPILTAYAGBEAOSRo SAUTROARNYCEPRSATn CTAICTEECMOMEPNLs ITA.NC.E.STAe TEMENT n rr Pa3ge
CSTEUDRY ITDEINTIFFIOICFATCAIUOTANH.E.TN.TIICIOTYN... PROJECTPERSONNEL...
LT LISTIOOA FFOFTS FAICB BGOLTNEUTSRL Ec ENTSSE .....co cecscs nomsmmm smnsso nooos s
LIST OFAPPENDICES...
1113
3200 OINBTJROEDCUCTTIIOVNEc ... ss
19 13
5600RDEFEERES NCECMAOTFREARNIIAALLYPTvIcC.TA..LI METOHoODNo..n.
1143
666432IPCrNIeSpOaUrMRatEMiDoOnoSGfEESSEtPVHaIYYn.ac .ndd.Faocrt.iro fci.cdactisoon nsSsosls sumtminoensss.e.s.oenssss: 1156
6:5 DescrofiInpstrtumienotanndOperating Conditions...
1176
7BO0 90
ERXCEPSOEUNRLCITMLSEU.N.ST.IALODNESSIGw N...ome sn ms ssss2019
10.0 RETOFEDATNAAT NDSAIMPLEO S...N .......c..ooooo
2201
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LI OFTSABLTES
Table Table.
SummaryofPFOS in Resgent BINS...
Page
23
Summaryof PFOS in Mallard Membrane BIanks.........c......... 24
Table IIL. Table IV.
SummaryofPROS in Mallard AIDUImen BISnkS.......v oe... 24 SummoafPrROyS in Mallard Yolk BIsIkS vc... 24
Table V. SummaryofPROS Recoveries in Mallard Metsbrae................ 25
Table VL. SummaryofPROS Recoveries in Mallard AIDUmen.................... 25
Table Table
VIL VIL
Summoaf PrFOyS Summary of PFOS
Recoveries for Week |
in Mallard Yolk... Mallard Membrane Control Samples.......
26 26
Table IX. 2SumBmaryB ofBROSform Week 1 Malo lard Membrr ane Samplese at 21
Table X. SummaryofPROS for Week 1 Mallard Membrane Samples at
Table XI. Summary of PROS for Week 1 Mallard Albumen Control Samples.........28 TaTabblleeXXIIIL..SSuummmooaffmPPrRFOOyaSSffoorrrWWeeyeekk 11MMaallllaarrddAAllbbuummeennSSaammpplleessaatt 61.72.p6pppmmia.i...2298 `Table XIV. SummoafPrROySforWeek 1 Mallard Yolk Control Sampls.............. 20 TTaabbllee XXYV.L SSuummmmaarryyooffPPRROOSS ffoorrWWeeeekk 11 MMaallllaarrdd YYoollkk SSaammpplleessaatt 61.72.6ppppmmaaii...... 3300 `TTaabbllee XXVVIIILLSSuummmmaarryyooffPPFFOOSS ffoorrWWeeeekk66MMaallllaarrddMMeemmbbrraanneeCSoanmtprollesSaamtples.......31
`Table XIX. Summary of PFOS for Week 6 Mallard Membrane Samples at
`Table XX. Summary of PROS for Week 6 Mallard Albumen Control Samples......... 32
"Table XX. SummaryofPFOS for Week 6 Mallard Albumen Samples at 6.2 ppm i... 33
`TTaabblleeXXOXXIILI. SummSaurmymoafryPoFfOPSfFoOrSWfeoerkWe6eMakl6laMradlAllarbduYmoelnkSContarolamSa1mp7p.6lplepsm.e.ai..s3343
Table XXIV. SummaryofPFOS for Week 6 Mallard Yolk Samples at 6.2 ppm ai . 34.
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TableXXV. - SummoafPrROySforWe6eMallkard Yolk Samplesat 17.6 ppam ..35 TTaabblleeXXXXVVILL SumSmuamrmyaorfyoPfFOPSFOiSn QiuniQluaAiIlbMUeMmEbNrBanIeTKBSlaks......c...o................3355 `Table XXVIIL SummaryofPFOS in Quail Yolk BISTKS cv... 36. ``TTaabbllee XXXXIXX..SSumumamrymoofafPPFrFOOySSRReeccoovveerriieessininQQuuaaiillAMIebtUaIbEtNa.g.e...................o.r.............3376 ``TTaabbllee XXOX(XX1I.L.SSuummmmaarryyooffPPFFOOSSfRoercoWveereikes1iQnuQauialilMeYmObrKan.e.C.o.n.t.ro.l.Soaomwplosr.s..r.........3387 "TableXXXII. SummaryofPROS for Week 1 QuailMembraneSamplesat
`TableXXXIV.TSuTmmBaBrMyoB fPFOSforWes ek 1QuailMemn brane Samples sat 39
`Table XOCXY. SummaryofPFOS for Week 1 Quail Albumen Control Samples ........39
``TTaabblleeXXXXXXVVILL.SSuummmarmyooaffPrPRFOOySSfofroWr eWeeekk|1QQuuaaiillAAllbbuummeennSSaammpplleessaat6.2ppmsi...40
TPP
sss 4)
`Table XOOKVIL. SummaryofPFOS for Week 1 Quail Yolk Control Sampls.........4.1
`Table XXXIX. SummoafPrFOySforWeek | Qual Yolk Samples st 6.2 ppm ai ......41
Table XL. SummaryofPFOSforWeek 1 Quail Yolk Samples at 17.6 ppm ai......... 42
"`TTaabbllee XXLLILL SSumumamrymoofafPPFrROOySSffoorrWWeee6k6eQQuuaakiillMMeemmbbrraanneeCSoanmtprloelsSaam6p.le2sp..p.m..a.i.......4432
`TableXLIL SummoafPrFOySforWe6eQuakil Membrane Samples at 17.6ppm a..43 `Table XLIV. Summaryof BFOS for Week 6 Quail Albumen Control Samples..........44
`Table XLV. SummaryofPFOS for Week 6 Quail Albumen Samples at 6.2 ppm ai.....44 `Table XLVI. Summaryof PFOS for Week 6 Quail Albumen Samples at 17.6 ppm ai. 45
`Table XLVIL SummaryofPFOSforWeek 6 Quail Yolk Control Samples ............. 45 `Table XLVI. Summary of POS for Week 6 Quail Yolk Samples at 6.2 ppm i ....... 46
Table IL. SummaryofPFOS for Week 6 Quail Yolk Samples at 17.6 ppm si.......... 46 Table L. Summary of Moisture Determinations for Mallard Membrane Samples..... 47
Table LL SummaryofMoisture DeterminationsforMallard Albumen Samples....... 48
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Table Table
LIL LI.
SummofMaoirstuyre DeterminationsforMalla Yolk Sampic......... 49 SummaryofMoisture Determinations orQuail Membrane Samples... 50
"Tebl Table
LIV. LV.
Summary of Moisture SummaryofMoisture
DeterminationsforQuail Determinations for Qusil
Albumen Samples....... Yolk Samples...........
51 52
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[I CentreStudy No. 023.063
Figure 1. Figure 2. Figure 3. Figured.
Figure 5.
Figure 6.
Figur7e.
LI OFFS IGUT RES Base
Typical Calibration CURVE fOr PROS...
54
Chromatogram Representing a 0.1 ng/L. standard for PFOS ccc 55
Chromalogram Representinga 1.0 1g. standard for PROS... 56
(CChernotmraetIoDgRreaamgReenptrBelsaennktAi,nagSeRte:a0ge3n2t6Bl0a1nkAfo)r.P.FO.S.cvvsrsmsss ST
CIDh:ro0m0a0t3o8g0r2aBmlaRenpkrAe,seSnetti:n0g3C2on6tr0o1l)Ma.llard Membrane for PFOS (Centre88
CphprboomfaPtFoOgrSa,m(RCeepnrterseeInDt:in0g00C3o8n0t2roSlpMkalAlaDrudp.MeImnjb,rSaent:e0Fo3r2ti6f0ie1d)wi.th 10 59
C4h1r6o.m2aptpomgraaim(REeDp1r-e0s2e6n3t-i2n08gM2a5)ll(aCrendtMreemIbD:ra0n1e03S0a9m2p,lSeeft:r0o3m2W6e0e1k0)1... 60
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LAISPTPOFENDICES
Page AppendiAx Study Protocol 00P-023-063 (Centre Study No. 023-063) and
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1.0 SUMMARY
`aClebnutrmeenAnaalnydticyaollkLasbaormaptloersiefso,rIntch.e(dCeentterrem)ineaxttiroanctoefd mpaerlfllaurodroaoncdtaqnueasiullfeogngatmee.m(bPrFaOnSe), according to protocol 00P-023-063 (Appendix A). Thelimitofquantitfaotricaocnhmatrix was 10ppb.TheLOQ forcachmatrix was determined in a method validation study performed at Centre (Centre Study: 023-027). POS ranged from non-quantifisble (where nor-quantifiable is defined as less than the `mcaolnlcaerndtramteimonborfatnhcesalmopwleesst,caflriobmratnioonn-qsutaanntdiafridab(l0e.1lenvgel/sm(L0) 3l5e5ve0lsngt/og4i3n4 tnhge/gmailnlatrhde albumen samples, and from non-quantifiable levels to 63,500 ng/g in the mallard yolk msaemmpblresa.ne PsaRmOpSlesr,anfgreodm fnroon-mqunaonnt-iqfuiaanbtliefialbelvee.lslteovel9s3.t9o n2g/0g90i0n tnhge/gquiainltahelbquumaeinl o`fsatmhpelseesr,easnuldtfsraroemrneopno-rqtueadnotinfiaawbeletwleevieglhsttba6s5s,.500 ng/g in the quail yolk samples. All Tahlbeumaevne,raagnedpyeorlckenstamrpecloevsewreierse9+1s%tanda6r%d,de9v1ia%tion1s6%fo,r aPnFdO9S3%in+ma1l3l%a,rdremsepemcbtriavenley,. The average percent recoverics + standard deviations for PFOS in quail membrane, albumen, and yolk sampleswere 102% 119%, 85%+ 5%, and 96+7%%, respectively.
20 OBJECTIVE
"Theobjectiveofthisanalytical phaseofthestudywastodeterminelevelsof paelrbfulmueonr,oaocntdaynoesluklufsoinantget(hPeFaOnSal)yitnicsal mpetheodoctfimeiadl,l"maDredteaernmdniqnuasatiilonegogf membrane, Perfluorooctanesulfonate in Egg Membrane, Ablumen and Yolk by LC/MS/MS".
3I0NTRODUCTION
ThisreportdetailstheresultsoftheanalyssforthedeterminationofPFOS inmallard and qual cgg membrane, albumen, and yolk using the analytical method entitled, "Determination of Perfluorooctanesulfonate in Egg Membrane, Ablumen and Yolk by LCMSMS". d`Tihriesctaonralsyitginceadl Cpoernttiroenporfottohceolstnuudmybwears00iPni-t0i2a3te-d06o3n. MTahrechex2p6e,ri2m0e0n1t,alwhsteanrttdhaetestWuadsy March 26, 2001, and the experimental termindaatteiwoans April 2, 2001.
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4.0 TESTSYSTEM
`Thecontrolmallardandquail eggmembrane,albumen,andyolkusedforthematrix
blanks and matrix fortifications were received on May 18, 2000 from Wildlife
Iwnetreermatiosnasl Ltiiodt.,gheEdanisftfoeenr,ednMtDmatarnidcesst:ored frozen at Centre. The following Centre ID's
MallaMradtMiemxbrane
Ca00r0e380I2D
MallardAlbumen
0003800
MallardYolk
0003799
Q`uQauialilMAelmbburmaenne
00000033779986
Qual Yolk
0003795
OnFebruary23,2001, 308quaileggsampleswerereceived. Thesamwperlesetorsed frfroozzeenn.unOtinltFheey wberre2ul4o,ag2g0re0d1yi,n1b9y4CmeanltrleadpeergsgosnnaemlploensFewberruearreyce2i6v,e2d0.0T1haensdasmtpolreesd wsetroreesdtofrroezdefnr.ozBaecnuhngigltshaemypwleerreecleoigvegdedwainsbdyivCiednedtienpteormseomnbnrelaonen,Maalrbcumhe1n,,y20o0k1,aanndd shell. Each componentofthe egg was given a unique Centre ID at the time of log-in. Althoughalloftheeggsampleswere receivedatCentre,onlythosedesignatedby the sponsorwereextraandcantalyezedd.
`Samplelog-in andchainofcustodyinformationcanbefoundinthe rawdatapackage assowcitihathitssteuddy. StoragerecordswilbekeptatCentreAnalyticalLaboratories, Tanscs.ocainadtedawtirtuhetchiospsytuodfy.thestoragerecordscanbefoundintheraw datapackage
5.0 REFERENCEMATERIAL
TEhnveiarnoanlmyetnitcaallsTteacnhdnaorldogPyaFnOdSSweraviscrees.ceivedatCentreon June3, 2000from 3M tSthaebicleirttyiwfaiscnaotofet aansasleysssiesd(ifnrtohziesns)taunddybwuatstuhseerdperfieorerntcoemeaxtpeirraitailowna.s Tshtoersedoalcucobridolifnitgtthyeo rrefeerencfemmaeatteerrriiaalieswlaissntednocbtelreoewle.vant to this study. The available information for the
CPoFmpOoSund Cont0re0C0o3n.y0o2lNo. TCTRCORONIoT.4~S~ Pur9i7ty9() Expi0n8t3i1o0nD1ate
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`The molecular structureofPROS is given below.
PChReOmSical Name ~~ = Molecularweight =
Perfluorooctancsulfonate 499 (CiFinSO5)
cFuz--o 1
Noobtien:edT,heisnpeoattraaslsimuomlepceurlfleuaonrodoscttaanndeasrudlffornoatmew[hCiacFhirtSheOsPKF]O,Smo(laenciuolna)risweight 538.
6.0 DESCOR FAI NALP YTITCAI LMEO THN OD
`AMnaelmybtriacnael, AmbeltuhmoednaenndtiYtoleldkb"yDLeterCmina/tionMwaosSfusPe/edrffloMrutohrioSosscttau"ndeys.ulfonate in Egg 61 Extraction Procedure
Egg membrane
A0.1 gsamplewasusedforthe extractionprocedure.Onemilloifmletihantolewars addedtothesample alongwiththre stainlesssteelbeads.Thesawmastphenlplaeced o`n mabeiadnbeaitear-tse~ct2a0te0po0nsirtptimofnior7~ffo2rumSimngiuntueetse.sE.aTchhesasmapmlpelweawsafsilttheernecdaenndtanrailfyzueogdnebady LC/MS/MS electrospray.
Egg albumen and yolk
A 10 g sample was used for the extraction procedure. Twenty-five milliliters of `methanol were addedtothe sample. The sample was placed on awrist-actionshakerfor 15minutesandthencentrifugedat ~2000 rpm for ~ 10minutes. Afivemilliliteraliquot oafltlhoweesdatmopilte.wEaascthaskaemnpalnedw0a.5s fgilotefrced aand rawnaablyszaeodddbenyd.LTC/hMeSs/MSaelemwctarsosspphraakyl.entehen
62 Preparation of Standards and Fortification Solutions
SLatbaonrdaatrodriseosl"utpiroontsocwoelr0e0Pp-r0e2p3a-re0d27o.n AMnaricnhdiv1i4d,ua2l00s1tocaks sstpaencdiafridedsoilnutCieonntorfPAFnaOlSytiwcaasl
preparedat aconcentration of 100 g/mLbydissolving 10mgofthe standard (corrected
for purity and salt content) in methanol. From this solution, a 1.0 pg/mL fortification
s1t0a0nmdaLrdwsiotlhutmieotnhawnaosl,prepared by taking 1mL of the stock and bringing the volume up to
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fTorhteif0i.c1atipogn/smtLa.nfdoartridfaicnadtibornisntgainndgartdhwevaoslpurempeaurpedbtoyt1a0k0minLg w1i0m th moeL ftthahneol1.0 Apg0/0m1L 1g0/0mmLLstwaintdhamredtwhaasnpol.repbayrtaekidng10mLofthe0.1 pg/mL standardandbringingto
gAs/emtLofssotlauntidoanrsidsnctohnetafionlilnogwiPnRgOmSannwears.preparedbydilutionofthe0.1 pg/mLand 0.01
TitelCone, (ug/ml) [o3l o0o1 o0o01 ofPROS
Volume(wl) 5 2 51 2 1
DilutotGeuld) 110000 110000 110000
Final Co0n.e0.05(ug/ml) 00000012 0000000025 00001
`The stock standard solution and all fortification and calibration standard solutions were. psrteopraerdatiionnacarnefbreigfeoruatnodri(n4thCer+aw2daCt)aawshseocniantoetdwiinthutshe.isreDpoocrutmentation of standard
63 Chromatography tiQumaenotiffiPcFatOiSon woafsP~F4O.4Smwians, PaeccaokmspwliesrheeddetbeyctLeCdi/nMtSh/eMcoSnterleocltmraotsrpriacye.scoTrhreespreotnednitnigo.n iottehtehaenarlesyutletrseitnesnetvieornatliomfe,tbheurtetchoveearmioesunrtespodretteedctneddtwheerreesotnwleyrseiglneisfictahnnttehneoluogwhetsot calibration standard (0.0001 pg/mL).
64 Instrument Sensitivity Tchoenscmaelnltesrotasftt0a.in0do0a0nr1dgamo/uontfmPFiLnOjSe.cted during the chromatographicrunhad a
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65 Description of Instrumeandt Operating Conditions Instrument: PE SCIEX API 3000 Biomolecular Mass Analyzer
Interface: SCIEXTurbolon Spray Liquid Introduction Interface
Computer: Dell UlraScan P1110
Software: HPLC:
WPiEnSdcoiewxsANnaTl,ysvtervseirosni4on 1.1 HewlettPackard(HP)Series 1100
HHPP VQuaactuPuummDpegasser HHPP ACuotlousmanmpOlveern
HPLC Column:Genesis Cs (Tones Chromatography), 2.1 mm x 50 mim,4 Columa Temp: 35C MoInbjieclteionPhVaosle.:(1A0):uL2mMAmmAceo tatein nASTiMtyu peIwm ater Mobile Phase (B): Methanal Flow Rate: 0.3 mL/min.
Tio me e%A %4B 0 0 10 0 0 10 n75o 00 440
Tons monitored:
AnPalRyOtSe neMgoadtieve Transi4ti9o9nM5o9n9itored Retention4T4i0me(min)
`TPuanerFialemeters
1SC-oTnotsrporlasy 'DFP-PD-eFcolcuusstienrginPgotPeontetnitailal ECPE-CEonltlriasnicoenPoEtneenrtigayl CXP-Colision Cel Exit Potential CentreAnalytical LaboratoriIensc,.
42s0a00 2531000 1E0D0
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DF-Deflector
3000
CEM-Channel Electron Multiplier
28000
GasFlows
Sat
Nebulizer Gas,
12
Curtain Gas
14
Collision Gas
4
TIS Temperature:
350C
6.6Quantitationand Example Calculation
Ten microfosalmplieotrcaelibrratison stawnerdeianjerctded intotheLC/MS/MS. The `peakareawasmeasuredand thestandardcurvewas generated (using 1/x weightedlinar regression) by Analyst software using at least five concentrations of standards. The concentrations for mallard and quail egg membrane, albumen, and yolk (ng/g) were determined from the equations below. `Equation 1calculatedthe amountofanalytefound(inng/mL,basedonpeakarea)using `tphreogsrtaamn.dTahrdencEuqrvueati(loinnea2cralrcegurleastseidotnhepaa ramem terosf)oangaelu nyetreaftnoedunbt dyinthneg/Ag.nalyst software
`Equati1o:n
Analyte found (ng/mL) =(Peak area -intercept) slope
Equatio2n: Analyte found (ng/g) =anal.foundsa(mnpgl/emwlt).x(gF)V(mL)x DE
Where: FV = Final Volume DF = Dilution Factor.
For samples fortified with known amounts of PFOS prior to extraction, Equation 3 calculated the percent recovery.
`Equati3on: Recovery (%)=
(anal.Found (ng/g)- avg.anal. inctrl (ng/g) x 100 `amount added (ng/g)
An example of a calculation using an actual sample follows: Mallard albumen sample Centre ID 0003800 Spk A (Set: 0326014), fortified at 10 `pbb with PFOS, where:
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pmeearkcaerpeat. slope
=So 3369 a
dilution factor
=
1
ng/gad(d forte levd el) = avg. amt in controls. =
10 ng/g. 0 (Not quantifiable)
fsianmapl lveolwueimgeht
=S 2l5omoLsg
Note: Not quanitfisb is define as les than the concentration of the lowest standard
(0.1 ng/mL).
From equation 1:
Analyte found (ng/mL)
From equation 2: = Analyte found (g/g) =
= [3369-9711
6700
036ngmL
(Q31060n38gm)Lx25mL1x)
`Fromeq%uRaetcioonve3r:y
= 897ngmL
= (89170nngglelex-0ngip1)0x0
Note: Thisexamplecalculation=wa9sd0o%neusingrounded numbers,andthereforemaybe slightly different from the values shown in the RAW DATA.
7.0 EXPERIMENTALDESIGN
fBoartcifhiseedtoatfksnamopwlnecscoonnsicsteednoftonarenradea~tg2ein0tosalnmapnslke,,so.nEeamcalhrsiaxmbplalnek,wtswoemxattirxabculsatniekndsg tbhleasnpkprwoapsrainsatleymzeetd.hodandthen analyzeidn duplicate. Also, ineachset,aTyp1 ieO
Centre AnsyticalLaboratories,Ic.
Page 1900102 000261
eeeeeeee------------ Centre Study No: 023.063
8.0 RESULTS
P`OmeSmbfraonuen,dailbnutmheen,reaangdeynotlbklablnaknskasraereligsitveedn iinn TTaabblleesII-TIVh.e TPhFeOSPFOinSthinetmhaelqluaarld d`meembtraenienc,steavleebruadmleonf,tahnedcoynotlrkolblmaantkrsicaerse,hgoiwveenveinr TfaebwlweesreXsXViIgXXnVIiIefnLoiuPgcRhtOaoSanlwtatesr the recoveries reparted. IndividualrecoveriesforPFOS in themallardmembrane, albumen, and yolksamplesare detailed in Tables V-VIL. The average percent recoveries + standard deviations for PFOS in mallard membrane, albumen, and yolk samples were 91% + 6%, 91% + 16%, aalnbdu9me3n%, +an1d3%yo,lkresspaemcptliveeslya.reInddeitvaiildeudalinreTcoavbelreiesXXfIorX-PXFXOXSI.in Tthheequaavielramgeembperracneen,t recoveries standard deviations for PFOS in quail membrane, albumen, and yolk samples were 102% + 11%, 85% + 5%, and 96% 7%, respectively. PcoFnOceSntrraantgieond froofm ntohne-qualnotwiefsiatbleca(lwihberrateionnon-qsutaanndtairfdiabl(0i.s1defnigne/dmLas) leslsevtehlasn thteo 434 ng/g in the mallard membrane samples, from non-quantifiable leveltso 3550 ng/g in the mallard albumen samples, and from non-quantifiable levels to 63,500 ng/g in the malyollkasamrpleds. `Theseresultsarereportedon a wetweightbasis. Individual results are listed in Tables VIII-XXV. Someofthe high levels of PFOS reported for some of the mallard egg membrane and egg albumen samples could be from possible contamination from the egg yolk of outer shell fPrFoOmSnroann-qgueadntfirfoimsbnioen-lqeuvaenltsiftioa9b3le.9lenvge/lgs 1i0n2t0h,e9q0u0anilg/aglibnutmheenqsuaaimlplmeesm,barnadnefrsoammpnloens., qauwaenttiwfeiaibglhetlbeavseilss.0In6d5i,v5i0d0uanlgr/egsiunltshaerqulaiisltyeodliknTsaambplleess.XXTXhIeIs-eIrLes.ultSs aro eorfeptmohretheeidgohn lceovuelldsobfePfFrOoSmrpeopsosritbleedfcoornstaommienaotfitonhefqroumaltheegeggmgemybolrkanoreaonutdeergsghealll.bum`TehnesSapmopnlseosr. Representative also informed Centre that the quail outer shells were not washed prior to separation of the intemal components.
`Theaveragepercentmoistures forthemallardandquail eggmembrane,albumen,and `yolk samples are given in Tables L-LV.
90 CONCLUSIONS
`The mallard and quail egg membrane, albumen, and yolk samples were successfully extracted and analyzed according to protocol 00P-023-063.
(CentreAnalyticalLaboratories,Inc.
Page200 102
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CentreStudy No. 023-063
10.0 RETE OF DN ATATANI DSAO MPLN ES `Whenthefinalreport is complete,alloriginal paperdatagenerated byCentreAnalytical Laboratories, Inc. will be shippedtothe sponsor. This does not include facility-specific raw data such as instrument logs, however exact copies of temperature logs will be `submitted. Exactcopies ofall raw data, as wellas asignedcopyofthefinalanalytical report and all original facility-specific raw data, will be retained in the Centre Analytical LaboratoriIencs., archifovrethse periodoftime specified in40CFR Part 792. Retained `samplesofreferencesubstancesarearchived bythe sponsor.
Centre AnalyticalLaboratories, Inc.
Pa21gof1e02 000263
-
OOOO
.
TABLES
Snir
srs
000264
--_--
CentreStudy No:023-063
Tablel. Suom fPFOm Sin a Reager nt Bly anks
-- SpooworD RegCeu"_ a3Btrie kA _ NeNuOmSm0bbtIe_ eAr BDaGt_ Dru6aetc0eeton _ WAD6Dmuu2iiMsesI_ FFoomAummiM_ idQygipeh
mm
RReegpuuBBllkkAA 0026600A ReguBakAt O20B
Ws6o0l1 M3e27200m 32600 2700
NNog No
mmo RRaeppnaBBulkkAAt O02200ICC a3sa8o0 33220000 uw RegaBkA 020M domi 32w00
NNoo No
TMTM m
RReepguuBBllkkAAt O022008 RepmBukA O20
zmo1 30
w32m80l0 vm0
NNGo NG
nnmm RReagpeoBBukkkAAr o0ZOcC 330010 030990000 NNoo
Bnmm RReegpmuBBllkkAAt OOIRAR 3320310 335000000 Bm RegBmxA 020 m0 3001
NNoo No
nMmm RReepgwuBuBkkAAt O02N0SA 3W2930010 33300000 NNoo
mmo RReegpemaBBllkkAAt 0O2S0BA 33950010 W35A10I0 NNQo
mwm RReeggwwBBlluakkAA' 0ONOBOIA 333900001 3441010 NNoG
nm RReaggeowBlBolkkAA OODBOOR 33000011 44210011 NNoo
mm RReegpwwBBulkkAAt OO%0BIC 3300000 4422000 NNoo
= +Duplicate jRcteiognmBekA noc STwAoNDoAlRDDEV4AIVAETRmIAOGNE:: NNNgGO
NQ = NotQuantifiable (lessthan the concentration of the lowest standard (0.1 ng/mL)
LOQ= 10 ppb
I
CentreAnalyticalLaborsoris nc.
Page B3of102
000265
ee --
`Cente Study No. 023-063
TableIl. Sponsor
n mm Mm
SummaryofPFOS in MallardMembraneBlanks
Centre n
OCBC2BBkAA* OoOmEaRBBlkasAkCA
Se Nomber
Exinction Date
Asabds Date
Amaiyte Found (ppb)
0O2E0IICC
2601 360
3zml 3200
No No
O0RW0OIC
Ol 3701
VL 39%
No NQ
STANDARDDEAVVIEARTAIGOEN:: ~~ NNQQ
TableIII. `Sponsor D Mm m m TM Mm
Summaryof PFOSinMallardAlbumenBlanks
Centre D
COOJOBBlWmEkAA* OOGXB0OBBUlskkAA* 0O0ONM00BBInmkkAAt
Se Number
Exactin Date
Asay Date
Amie Found ppb)
OOIRMAIA e32660011 3w2e52i0l1 NNQQ
0072008B
3201 3201
3282901 IML
NQ NQ
OOIC OBC
3001 M001
42L 4am
603 659
AVERAGE: 294
STANDARDDEVIATION: 262
Table IV. `Sponser
D ry wm m mm
SummaryofPFOSinMallardYolk Blanks
Centre D
OCOMOMOBlMmkAA OMONOBBnA kAr
St Number
Extraction Date
Anais Amayte Date __ Found (ppb)
00N26I0IBB 23620601l 332z0LM NNoQ
OOOWI0IAA
W201 327m
3281 wor
957 90
AVERAGE: S48
STANDARDDEVIATION: 487
*Duplicate injection
'NQ=NotQuantifiable (less than the concentration of the lowest standard (0.1 ng/mL) LOQ= 10 ppb
FwoarsruesseidduteoscraelccoulradteedtahseNaQve,rahaglefoafntdhsetacnodnacredntdreavtiiatoinoonf. the lowest standard (1.25 ppb)
`CentreAnalyticalLaboratories, Inc.
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Centre Study No: 023.063
TablVe. Sponsor IY m mwmm Mm mm m mM m
Summary of PFOSRecoveries in Mallard Membrane
Centre D
Set Number
Extraction Date
Aualyss Date
Amt Added (ppb)
cCOOmDmMR2SSpEKEAA
0200IC 0260IC
3ae0l 32601
2700 32700
0 10
OOONSMRRSSKKBBe GORBGGIICC 3322660011 332270010 11000
GOONBm2RSHSKpAAT
GR2OIC GR0IC
3701 3701
3900 32901
10 10
oOoNoBmoQsSpXkBBe
GR0IC owoic
20 mol
32901 nomi
Ed 9
STANDARDDEAVVIEARTAIGOEN::
RELATIVE STANDARD DEVIATION:
* Recovery
5 o aa 0 0 9s 9% 91 76
TableVI. SummaryofPFOSRecoveriesinMallardAlbumen
`Sponsor D
Ceatee D
Set Extraction Analyse Number _ Date Date
Amt Added (ppb)
ms on
OONOOOSMPOSKPAAT
GR60IA ORGIA
32601 32601
326210 326271
0 0
wm Mm
GOODOBNOOOSSKpKKBBT
ONOIA OGOIA
3260 32601
3262701 3262701
9 %
mMm
OODOSNOSOOSSpPKKAA GORR2070IIBB 3322770011 33228822990001
0 10
mm nm
OOONIOOSSKKBXB*
GRI0IB GRO
32701 V20l
3282901 3282901
9 9
mTM COOODMOOOSSKKAA ODOOINCC 333000011 44220000 1100
Mm nm
OO0NSJO0OSSpKKBBe
(XOIC aoc
33001 dAomi
4200 4201
one ours
AVERAGE:
RELATIVESSTTAANNDDAARRDD DDEEVVIIAATTIIOONN::
* Duplicate Injection L0Q=10ppb
% Recovery
o1 ot 1207 105 to %5 6 % % 91 16 17
`CentreAnalyticalLaboratories, Inc.
Pa25gof1e02
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Centre Study No.023.063
Table VIL SummaryofPFOSRecoveriesin Mallard Yolk
Spomor
CeInDtre NuSmuber ExDmactsin ADmuiss AddeAdm(pb) Res*owsy
NNao CGOMSWSSKEAA 0022606i0B 636001 3320000 T1o0
T1o4r
NNoo OO0NSUOSSKKBBe o0Rn60eB ssuull a2m0oln 55%
%
NNee GOOmMmOOSSRKAAT O000I1AA 2l0L1 3302880000 0
n
NNo ooCOWsOSeKB OoRm0IaA Wdoe 3o2w0e0 95
58
STANDARDDEAVVIEARTAIGOEN:: 9133
RELATIVESTANDARDDEVIATION: 16
TableVIL SummaryofPFOS for Week 1 MallardMembraneControl Samples
Sponmsor Centre NuSmtber EEollmeeaone oOWOE0 | 00200IICC BBolLmOesOaoMse OolEs O0RIeICC EELLossoze0s8ese oOmse 0OR6eIICC EElGmeeaaomswee oONiE oGR0AiICC EElLmMa oOldiIes OO0IR6CIC +Duplicate jection
EDxaitmscn ADmaites FoAumdi(toept) Mweeue 3samn NNoo e3so0L 3m0L Nnee We60101d3L NNoo awesoul vmaol nNeo saswoi 3a0mn0L NNeo STANDARDDEAVVIEARTAIGOEN:: ~~ NNQQ
'NLQ 0= N1Qo0tQp=upabntifiable essthan theconcentrationofthe loweststandard (0.1 ng/mL))
CentreAnalyticalLaboraoris, Inc.
.
Page260102
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Centre Study No: 023.063
TableIX.SummaryofPFOS for Week 1MallardMembrane Samples at 6.2 ppm ai
Sponsor Centre
m
TBolcmaeeaameess o00o
BEOlLOe62u0Me6 OOBIEG
B EOLL 26I0O8R OO3BI00
EEOOLLOC3Sa00MMT7T OOBBIIS
EEOlLm6eS2a0uMEs oOBwIsE
NuSmeber ExDtaratcetion ADmatei FoAunsdiaept)
0ORIOICC J3aaeso0ll vwaaml tTeeses
OORIEICC
ew deol
3am 3m
6s 62
OORIGICC dseooll 3aol 7212
OORRGGOIICC 3s6o0l 3371m 3219
oORnGeOiIcC adessou 3a7m0 s5s4
STANDARD DEAVVIEARTAIGOEN:: 141021
TableX. Sum ofPmOSa forrWeey k 1MallardMembrane Samples at 17.6 ppm ai
Spossor o
Centre
St Number
ETooLlOmseza0ilsSes OORlIeG | O0RRORIICC
EELls;a0eme0sr OoBlIaS OORRMOCIC
EElmLeE rs oOmZo OoRa6oIiCc
EEOLO23L208]51 OOINONSS6 GORRe6O0IICC
EEllsmes ooomme o0RnRiIcC
Bxncin Amis Date Dats __ ndeesool 3wm0l 63s00L 3371m dwaeeonl wwem 330610 moLl meeom wwmml
AVERAGE: STANDARDDEVIATION:
Amite Found ep)
226000s0 22556 709 amm0e 0s021 2S3211
+ Duplicate jection
10Q= 10ppb
R+epProessseinbtlaetciveorequnesttwerabeynyomotlukisoerdontuotceaalschutellatleciothmepoaovnenernatgoefasnd sa tandaam nrddadsepvpieartl iSopno.nesor
CentreAnalyticalLaboratories nc.
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Centre Sdy No: 023.063
TableXI. SummaryofPOSforWeek 1Mallard AlbumenControl Samples
Sponsor I
E01-0263-20852.
EEB01m-i02e6G3n-o20e8o5e2% EBmeiosGse EBlimmeeoaswr Boma Boer
Centre
0103201
0OO1oo03mm20ss1 OoEnD OOmNeG oowesess
NoSmbter
0326014
0mG32uR60aA14 GGORAA GORHGINAA mpeAin
ExDuoen ADmaited__FouAsmdipepb)
32601 3262701
NQ
sS6o00l11 we26e2rz70o1 NNNQQO M3Se0o1 W36622001 NNGO nSeOoL M3662M0L NNGO swoeln Wsemzool NNQg
AVERAGE NQ
STANDARDDEVIATION: Ne
TableXII. Summaryof PFOS for Week 1Mallard AlbumenSamples at6.2 ppm ai
Spomor Cen"t3re NuSmber BDaumscon ADmuies FoAwmdiipeh)
r Tomese On URI as01 ezidl 24
EElpmeeasss OONWTT omnAe oSs0o1 dWasszaomll 71580
EEollmmaesnee Oo0o1 EDEN OIS
mOeReiInA GIA
eSOwL ssazm We0l W62m
SS6m7 S76
|
BEolmLeiaMows OOOoNNSNs RO2OOIAA MMee0nl WW662O1L SS9S8
|
Eolmeous owns oma wen eAVrERAmGE: 564
| |
STANDARDDEVIATION: 130
Duplcte Tjction
'LN0QQ==N1o0tpQpuabntifiable (less thantheconcentrationofthe lowest standard (0.1 ng/mL)
CentreAnalyticalLaboratories nc.
Page2801102
000270
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Centre Study No: 023.063
`TableXIII. SummaryofPFOS for Week 1Mallard AlbumenSamples at 17.6 ppm ai
Sponsor Centre NuSmeber EDxaitnscin ADmuiiss FoAunmdi(eopt)
ETolr;oweesaamoesses OOWlRoDm GORR0MIIDD G326U01 wdaaasawri wasosmo
BEOLOO2L308DS6 001002321177
OoBx0oIiCc
soon IOI
aol 4200
13850 136m
EEoOLLO6sI3200M8SS66 OOI0RRTDDwp OGGIOICC 3336000011 442200 Taiesee
ElGa060 Elmeaes
OINISH OJI3
ORMIA ONIA
G3D60Ll a3assmii
B24S7
EEOOLLOO62362320068SS77 0O1l003s222211 oolAa oseoml e dasmzm 8B3s
EEOLLCEIR2a00sSl1 O010N31S97 OooIlA a6001 e el l @T7O
EBnoolcmzioaeessst oOlloXwaaSs oORnGOsIA nIe60oI wdepie 11997s
STANDARDDEVAIVAETRIAOGNE:: 222250
Table XIV. SummaryofPFOS for Week 1 Mallard YolkControl Samples
Sponsor Centre
EToLoo220a82e OODIEE
EEOoLlOcGsaIo0e8sSsI OooRm;
EEoOLL26eII220086SS4e OOlI00RS0066
EEl;le2e0s OOINONSSS
EBlmomomesrsoomne
+ Duplicate jection
sa Number GOReBl oOnNGeOIsB
OORRE6OOIIBB OGRR60IBB o2eBm
Estacion Due
Amys Dats
Analyte Fousd Gp)
wdae6s0wL aILm NNoo
63ae0on 3d2a7m0m1 Nxoo
33aasso0ll aomll NNoQ
33660010 332m0 NNoo
eeeon omll NNoo
STANDARDDEVAVIEARTAIGOEN:: ~~ NNQQ
'NQ = Not Quantifsble less thantheconcentration of the lowest standard (0.1 ng/mL) LOQ= 10ppb
R+e+pProessseinbtlaeticvoentraeqmuiensattiwoenrebynoytoluksoerd tooutcearlcsuhlealtlectohmepaovneernatgeofansdamsptlaendaanrdddaesvpieatriSopn.onsor
CentreAnalyticalLaboratories oc
Page29.1 102
000271
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Centre Study No: 023.063
TableXV. SummaryofPFOS for Week 1Mallard YolkSamplesat6.2 ppmai
SponDsor caTrDe NSem BDnucein ADmuei ouAnsdiaep) ETloelamomasne OoNN GOM2GO0ID wWa6e001r I32mraasmdl 22457800 EEllmeemsaiwse oOlTae O0NOGDD a6u0l1 3371as2e0l 1122510000 EBllmoeeer ooluouss 0O0RDD 3ee0l r37a28s00 0100 EBlomleesr oOlIoEs O0NRGIDD asbull 3amassell 110150000 EEllm6eaMmss OoiRe RpAeInDd 6s0e1v 3o2sse 1122110000
STANDARDDEAVVIEARTAIGOEN:: ~~ 4344500
TableXVI.SummaryofPFOSfor Week 1Mallard YolkSamples at 17.6 ppmal
SponDsor CentDre BToLrcQae@ssamee| OOoRulMs EEOLLO;GSGZS0Z805S0T OO3O0S EEDLl232m08s57 OolWowSis EBoLicIasAaoSs oOleIs Elsa Ow
NSeum BxrDmuceton 00R%66IIDD wpa0e1 OORRGAOODD 6Ie0bl OONREeOIDD sWoalo oORnGIoD wasoo Gu6ND sul
ADmuiee 3w17a2a0o0n Irraamei 3r1a2sao0ln n21a28e00 3zashl
FoAunsdioept) 330030000 "0a0n0o Ss6ma600 5p10y0 si00
STANDARDDEAVVIEARTAIGOEN:: 4182500000
Duplicate recon
.
10Q=10ppb
CentreAnalyticalLaboratories, loc.
Page 300102
000272
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Centre Study No: 023.063
Table XVII. Summaryof PFOSfor We6Meallkard Membrane Control Samples
SpIo"or Contre BE omaE ss E0) 10364 BBoLwOeGs O010l37e68 EElmwess 0o1l0s3 EEllmemrss oolsken EBLoOlE2e9s7 _ oO0li00s5T7E800
NumSbter oaozoo aaomco owzzooicc oownoioc oGrmocce
EDxaitmecon ADmaiten FoAwmdiGep) vzoemn vaesll NNoo vvoomm wwmmll NNoe vvozm wwnmt NNeo wwzzm wvmm NNoo swmm nwoomm NNoo STANDARDDEVAIVAETRIAOGNE: ~~ NNCQ.
TableXVIIL Summary of PFOS for Week 6MallardMembrane Samples at 6.2 ppm ai
Spomor Centre
m
B BOLL;O O0l100s3T7O0s8
BElLLesO2i0G0 0011003379966
EEoLloesmaoease O0L100sT5A7s
BmLiRmSAeSsI 0o0l36e7s6
EE0l3m0s9 0011003374488
*Duplicate njction
NumSbeer owmoic mozoocc aaoocc Gamoo oawoocc
EDxaitmeco ADmaites FoAwmdi(pepb) vsmmn awml 0085 mwomm v3mm 555533 wamee ovomm 558577 vaommo waem 1m6e0mee vvoomm wwomm m582 STANDARDDEAVVIEARTAIGOEN:: 7117.03
'1N0QQ==N1o0tpQpuabntifisble (lessthantheconcentration ofthe loweststandard (0.1 ng/mL)
R+e*prPeossesnitbalteicvoenrteaqmuiensattwieornebynoytoulskeodrtuotcearlschuelllactthoemapvoenreangteoafnsdasmtpalnedaarndddaesvipaetriSonp.onsor
CentreAnalyticalLaboratories nc.
Pa31gof1e02
000273
J ----
Centre StudyNo. 023.063
`TableXIX.Summaryof PFOSfor Week 6MallardMembraneSamples at17.6 ppm ai
SpIo"ns)or Contre ETondoass 0ol00s3Ts6e mmiiawmsoe 0o05s82 EBLomsas0n OO0000 BELslsmzis0e olonlssesoose EoELoolamesss20lo000s 0o0w3s8s0o8s
NuSmtber Tmzioccso wazzooccoo oomzooccoo oozoocoo oozoocno
EcDukeo ADmues wwmol snonm vwoon 3s9o0m1 vvaamm 33990001 vvaamm 330000 evnm 3s0a90v0 STANDARDDEAVVIEARTAIGOEN::
FoAwmdieey) oaos 2065 330 0a6 220 63102
TableXX. Summaryof PFOS for Week 6MallardAlbumenControl Samples
Sponsor
Emaam
E01-0263-20993%
Centre n 006s
0103765
MeStmb wom
0327018
EDnuec ADmuisk FeuAmdipeh) Al wss0l Ne
Ec
328-2901
Ne
E01-0263-20094.
E0E1-o0l26m3-s2a09m94s* Emiomness EElnmeeras _Enocowwr ----
0103769
0o10w37s69 001003793 o0lss ows
032701
0oo32zo70oo18 Goanoon ozo owls
3m 328-2901
No
E vzL zm 3ms28-s2m9m01 NNNoee wamme mmaaem NNeO azo aman NO vmoy smAVsEwRAoGlE: NNOo. STANDARDDEVIATION: NO
'NQ =NotQuanifsbl essthantheconcentrationofthe loweststandard(0.1 ng/mL)
LOQ = 10 ppb
Centre Analytical Laboratris, nc.
Page 201102 nNN0274
Centre Study No 023.063
`TableXXI. SummaryofPFOS for Week 6MallardAlbumenSamples at 6.2 ppm ai
Spomor Cenmtre EEol6as9ao9n o0I0SsTOD EElLmeSr 0011008379917 E EOLOLGSZ0E988 0O1l00S3TasS BBoolweenn 0001%07% sEDoLOl620o98s9 Ooloonna
NeSmbee ExDiumcsion ADmuien FoAumdi(pey) GGroois smainn smasle 568 2 oozzoosB oomn mmaaem 1s%o oazzoo osnm mmsee 66 6 aomozs onoen mmaaem 1es ooooln somm soasem ssnn
STANDARDDEAVVIEATRIAOGNE: 0751897
`TableXXIISummary of PFOSfor Week 6Mallard Albumen Samples at 17.6 ppm ai
Spoor Gennre EBOLoOaG2o0L 0O1l0088T7SSTT EBoomlasd0ar oolloss EBOLoOGlZIgO0 o0ll038l0L EEoLLOmeGs2a0I0s0 0o1l0os3k0o5s BoLoeszion olsun Eom 008 otosan * Duplication
NoSmebe ExDmuceim ADmuei FoAusmdinpeh)
Goozlos swzmimln wssmdel 2u8o3
cazmooms mson asmseo aulr
oooolm vmoL wssmzem 119974
oomwosm zwom asswme 224s0
oom oon
omomn sssszwm
1192a
STANDARDDEAVVIEARTAIGOEN:: 421023
L0Q=10ppb
CentreAnalytical LaboratorieInsc,.
Page330 102
000275 .
--_-------- Cone Study No: 023063
`Table XXIIL Summaryof PFOSfor Week 6 Mallard Yolk Control
Samples
--D ConDte Brlgmeaame oonvaa BBmww oovvee BBiimaeasmes momoom BBilwaeasrss oommee Emimnmuem ooormn
MeSmtber BuDuer.n AmDiunre sAemmiie) owoom meel avmeel MNae wowooiA eell mvemle NNae oowowiA uemle aammel NNee mowooaA eell uvmaem NNoe owwean umel ummeel Nnee
STANDARDDEVAIVAETRIAOGNE: NNao
`Table XXIV. Summaryof PFOSfor Week 6 MallardYolk Samples at
6.2 ppm ai
E `SpoDnsor. CenI Dtre MSeembter Due Extraction Dur Analysis SoAnmaliytre)
Bmleasmoe oolwoew GGraouiabb mamml suemle 1ow5e0
Diimmees ooowme Gcaobooalml daemle lookwo
BSlemeaaenm oowveas Gow;oOiAaD> dvomm oeoe lieo
EBGimmaemaiwe oomoewne GGwomolAAD> damoll doeel mloeee
I
game ou moon wml AoVERoAGE: s161o00
[E--
STANDARD DEVIATION: 6330
!
'1N0QG==No1t0Qumantifiable esthanthconcentrationoftheloweststandard(0.1 ng/mL)
CentreAnsyial Laboratories, loc.
Page of102
02786
EE
--
CentreStudy No. 023-063
TableXV. SummaryofPFOS for Week 6Mallard YolkSamplesat 17.6 ppmai
`Sponsor
Centre
EBoLmOeaL91e oOll0o3sTsSsA
EELlOmG2e9r0 0011003377500
EELOlG2mI0s02 o0l1o0s3m9s8
EE2l6m2s0 oomie
EE0OLLUOEGSI21Z0I00660 00110033880066
NSuemtber oTOoAdD oowooluD> oooopp oooopp o oood
EDxtartacetion AnDauliyssis woolm wweeel zwolm vweeml zzoon dweell ooll wwemll seoly nOoLm STANDARD DEAVVIEARTAIGOEN::
`Table XXVL SummaryofPFOS in Quail Membrane Blanks
FoAunnadlyptpet) a4m0 essoow ssmsoo deein adsewoeo $80877000
Sponsor
CenotDre
NSumebter ExtDraatcteion AnDaluyesis FoAunnadlyptepb)
Wo COLTSBMEA| GIOIA 301 36101 No
mTMo CNOITSSSEBBkKAAC OONZOSOIBAR 3300010 340210000 nNeo
nm
ooovosBmkAt
oneBR 3001 AVoERoArGE:
No NQ
STANDARDDEVIATION: ~~ NQ
`Table XXVII.SummaryofPFOSin Quail AlbumenBlanks
`Sponsor CentDre. NuSmebter ExtDraucetion AmDluyess FoAunnadlyoteod) wmo OOOONSBGaBEkAA| OO0MIBB 33220810 330000001 Nnee mwm oOooNsSmeGbBimkkAAr OoNsGoInAa 3s6e0m0 4aiAmI nNeo
* Duplicate jection
STANDARDDEVAVIEARTAIGOEN:: ~~ NNQo
LNOQQ==No1t0 Qpupabntifiable (lessthantheconcentraotfitohneloweststandard(0.1 ng/mL))
Centre Analytical Laboratories, Ic.
Page 3sof 102
000277
EE
CentreStNuo:0d23.y063
TableXXVIILSummaryof PFOS inQuailYolkBlanks
Sponsor Centre
Su Euros Amis Amie
Ir)
Number Dae Due Found gph)
Wwwo oCmOTTSSBBuAkkAA| GOZMOIIAA 332288000 3305000011 NNoo
mwwm oOwMmIsSBSBuumkAAr o0mSSeOB 3o9mm WmaMnOo NNDp
STANDARDDEAVVIEARTAIGOEN,: ~~ NNQQ
`TableXXIX.Summary ofPFOSRecoveriesinQuailMembrane
SpoIn)sor CeInDtre NSetm ExDucel ADmuiss AddeAdm(op) Reco%very
mwoo OGHSTEASSAETA OONS00IIAA VSO1L WBAILOLL m1o0
0104
mwom cClTISBESSNKDBe 0O0I1AA S9O0L O33L01 o8n6s
r5y
wmoo oCmOSWEESSEMAA OONBOOIIBBRR 33330000 44220000 110
1h1m6
wnmm eomessmsksBne oonoon 3v0i0o 4a2o0n1 5%8
110018
STANDARDDEVAIVAETRIAOGNE:: 11012
RELATIVESTANDARD DEVIATION: 11
*DuplicateIfecion
'NNDQ == NNootpQeuaakndteitfescbtled.(lessthanthe concentrationofthe loweststandard(0.1ng/mL) L0Q=10ppd
CentreAnalyticalLaboatoris, oc.
Page 36.1 102
000278
--------------------------------------
ere StyNos023-063
`TableXXX. Summaryof PFOSRecoveriesinQuailAlbumen
Spnoor CIoDe NeSuwb BeDiumcsim ADmuiiss AddAeimGpd) mw GGTeSSSKA GGOmB 3os8o0i 3305000011 T1o
na
0003796SpkB 032801 32801 35001
9963
-Mm 00C03M7M96SSpEkAB 0O3N28D01AB 332080001 354010011 19963
nmnmwm 0o0o0oO3m7m9m6eeSpsSkpBAe* om0m3o3m0i0n1AA 3do/3o0m/o01 auwolur
10
STANDARDDEAVVIEATRIAOGNE:
RELATIVESTANDARDDEVIATION.
Hea"rs TM
0
=) 5a8t8 55 &
`Table XXXI. SummaryofPFOS RecoveriesinQuail Yolk
Sponmor CIeDe NaSmwber RaDcuein ADmuisk AbdAedmop) Re*sor
nmno coms 0003795SpkA 0o3w2m80i1aA o32w80e1 y3o0u0l1
1100
110024
nannm 0c0Co0O3o7mS9s5SsSEkpkmABe oo0um32so8i0B1aA 3ou2w80me1 olw30o0e1 9s60a2s3
09n%1
nwmm oOXwweessSSKkABY COmmoeBD mmonl aalnoanm owlon n54
nm coomsswpe owsop doom wim AoVwERsAGE: %
RELATIVESSTTAANNDDAARRDDDDEEVVIIAATTIIOONN:: 77
+Duplin
L0Q=10ppb
Centre AnalyticalLaboratories, Ic.
Page 370f 102 000279
Ceatre Study Noi: 023-063
Table XXXII SummaryofPFOS for Week 1 Quail Membrane Control
Samples
`Sponsor D
Centre D
set Nomber
Extraction Aubpsis Amlyte Date Date Found(pb)
EOL0255.20506 0101987 E0L0259-20506% 0101987
omsola ool
wool vel
3600 38
S20m se
E0LO2S920507 EDLO253.2007%
0101991 0101991
oma sol BIO onsolA sol BIOL
Ng No
EOLOSO-0520 E0LO259-205200
0102043 0102043
omsolA onsolA
ase se
BIOL 3601
NQ No
EOLOS20S2 0102047 EDLO2s00s21% 0102047
ool mola
vol sw sol 38M
~Q No
E0LO259:20509 E0L02S920509
0101999 0101999
sonosloal swomn 33BnUwOoL
NNQO
AVERAGE: NQ
STANDARDDEVIATION: NQ
Table XXXIIL Summaryof PFOS for Week 1Quail Membrane
Sponsor
Samples at 6.2 ppm ai
Ceatre Set
Extraction
Amalysls
Aualyte
D
D
E0L0259-20526 0102067
Number osoD
Date wenn
Date Found (ppb) anal 18800%
E0LO25920526" EOLO2SO0S14
0102067 0102019
onsoD omsolA
sel sel
4n0l BIOL
170
E0L0259.20514% 0102019 BOLO) 0102071
omsolA ool
wvoL oo
BIL BW
El m
EOLO2S9-20527% EOLO250:20515
0102071 0102023
oso omsol
aso sel
BIOL 300
116 m
EEoOLLOo2Ss9s20s5l28s 00110022002T3S momsoolpA oseIl a3noBn 1m1S
E0LO2S520528" 0102075 OonoOID neon AaVaERpAGE: li14s0o
|
STANDARD DEVIATION: 253
* Duplicate Injection
'NQ = Not Quantifisble (less than the concentration of the lowest standard (0.1 ng/mL) L0Q= 10 ppb
+= Possible contaprmiionrattoiosnepbayraytoiloknoorfoiuntteerrnsahlelclomcpoomnpeonntesn.tAofspseamrpSlpeonsisnocre outer shell
-
Centre Study No.: 023-063
`Table XXXIV. SummaryofPFOS for Week 1 Quail Membrane Samples at 17.6 ppm ai
Sponsor Centre
D
D
EEO0L1O022S5S92.200552299%
0102079 0102079
EE0OLL2O8S92200553311
0102087 0102087
EED0LL20SZ9S292005533220 00110022009911
EOLO259-20518 E0LO2S9205180
0102035 0102035
EOL0259:20533 E0L02S9205330
0102085 0102095
set Number oso nso nso oso oonssooD oso oso onsoID oo
Extraction Auslys
Date Date
sol nani
W901 wow
anal 4n2nl
wow sol
1200 ann
op ase
anal nan
ase anann
ws woe
41200 nam
STANDARDDEVAIVAETRIAOGNE::
Analyte Found ppb)
20900 2000 11000 11100 16300 1390 12500 12600 23 69% 13100 4730
`Table XXXV. SummaryofPFOSfor Week 1 Quail Albumen Control
`Samples
Sponsor Centre
'y
n
EEOOLL0O225595-:202500066% 00110011998888
EOL29:20507 EOLOS920507%
0101992 0101992
EE0OLL205S992200552200%
0102044 0102044
EOLs50s21 EOLOSS20S21
0102048 0102048
EOLO255:2009 E0L0259.205090
0102000 0102000
set Number opm oso ops 0B oz0B oonssoisB oossB oop
Extraction Avalyss
Date Date
esol awol
30000 36000
mmooll 3385000000
wel 36001
womwooll s36o00n1
msooll 3365000010
wer
soo AVERAGE:
STANDARDDEVIATION:
Analyte Found (pb)
No NQ NNQQ No NNao NQ NQ NO NQ ~~ NQ
*DuplicateIjction INQ = Not Quentifiable (less then the concentration of the lowest standard (0.1 ng/mL)) L0Q= 10 ppb
CentreAnalyticalLaboratories, Inc.
Page39.0f 102
-_
Ceatre Study No. 023-063
`TableXXXVI. SummaryofPFOSfor Week 1 QuailAlbumenSamples at 6.2 ppmai
`Sponsor Centre
D
D
EEODLIO022S9O.22005522660 00110022006688
EEDDLL0O22sS9O--2200551144% 00110022002200
EEOOLLO02ZSS9O220055777 00110022007722
EOLO2S9-20515 0102024
EEOOLLO2S5O0:22005512S8*
0102024 0102076
= E0L0250.20528" 0102076
sel Nomber os0B Ons oonsnolsB omsB oonsm0B osiB ou0B
oso
Extraction Amlpls
Date Date
3201 wel
3360003310010
30l 3as01
336600331I0011
3n801 mol
3300033110001
wel 3003101
wel awoL
33031010 031
amo
3n03uol AVERAGE:
STANDARD DEVIATION:
Asaiyte Found (ppb)
603 54 20 2m 204 53841 608 99 gare 438 1.61
Table XXXVILSummaryof PFOSfor Week 1 QuailAlbumen Samples at 17.6 ppm ai
`Sponsor Centre
D
D
EOI0259-20529 E0LOZSO20529
0102080 0102080
EOLO2SS0531 EOLO2S9-20531%
0102088 0102088
EOEOLL20s2Ss902055302
0102092 0102052
EOLMSS0S18 EDLOSSSIE
0102036 0102036
EE0OL0L2OS0S-O200533330 00110022009966
--
~
*Duplicate njection
sot Number ORB oomss0iBB o;sB onsIB oOsnsE oRsIB ORME opp
Exmcion Date
Amis Date
3280L wel
3503101 3803100
30s0L 30801
3603101 3503101
sol 3003101
2aem0oLr 3363003310001
3mOL wel
33060033110010
ower
3n0suol AVERAGE:
STANDARDDEVIATION:
Awbte Found (ppb)
9 3 997 105 107 2130 316 907 101 160 892
L0Q=10ppb
CentreAnalytical LaboratorieIsn,.
Page 400f102
000282
ee----------
Centre Study No. 023-063
`Table XXXVIIL SummaryofPFfoOrWeSek 1 Quail Yolk Control `Samples
`Sponsor. Torsone E0BB 1-0i259-L s205s06* BBiisHa iElmss EBLvsoez0ss0
Centre.
oles 01001010101919898589 oollss o0lo2s0ss ootio0iisseerT
MSemebter moa 0oo32uu8oo0li1AA4 ommooll oozoAl oonpssa
Due Extraction awl
Duc Analysis 35001
FoAunadlypteh) No
0 a28w0n1 33330000000010
NNNooo
mmoorr 3300mm Nxoo
namwoor a3n0o0l0 xxo
wwwmeww 3n0o0o0n oNo
STANDARDDEAVVIEARTAIGOEN:: ~~ NNOQ
`TableXXXIX.SummaryofPFOS for Week 1 QuailYolk Sampalte6.s2 ppm al
`Sponsor
D Centre.
BBoicsats OOlWe
EBLmiSSs0sSu OoHoVn
EB LsSLTe OoIoGd
EEiLmsoSsiIs oOlpmm
EEmwonws OoAoDm
+ Duplcste Ifction
NSumebter UGROMIDAD OORHM0OIAADD GoHMOOAADD oOmRoAIaADD GomRoAaDp
Due Extraction AnDaulyissis FouAnnadlytpeh) mw0o U01L0 1177500 awo0n W3oML B#I0D wdaoto mMmI L12a0i0 aMmool dAuOoL a1m3e0 mweLe MoOLoo Lo STANDARD DEAVVIEARTAIGOEN:: 132165000
'NQ =NotQuantifiable(lessthantheconcentrationoftheloweststandard (0.1ng/mL)
}
LOQ= 10 ppb
Cente AnalyticalLaboratories, Inc.
Page dof 102 000283
_--------ee------------------
Centre Study No.; 023-063.
Table XL. SummaryofPFOS for Week 1 Quail YolkSamplesat 17.6
ppm ai
`SIpo"nsor
D Centre.
Bolsa 0102077
EBollmssa oolsasn
EEoLlssmasse oolmse
E Emn monss oolms
BEoonLasssa0ssins oolooamss
Elms oon
MSeemtb.er ToB0IAD GGuooaAdD GGuaooAADD GGuaosAoDl GGuomso0AADD omsoAD
ExtDraucetion AnDaluyesis FouAnnadlyGtpe ) BOL 36IOL 42500 wmweorr wamonn a00 wmeoll aanooll u3e0 swwoo r lmno 33si00 wnesOlL uOmLl 4000 moi sum ais STANDARDDEAVVIEARTAIGOEN:: 346173000
TaXLLb.Sulmmaeryof PFOS for We6eQuakil Membrane Control Samples
`Sponsor
Toros E0B1-o0L25m9-s20m73s5* BEoLLosSoa0T6e BELimmHamn0 EmoiLomsso2a0nn0sr ELT
+Duplicate econ
ICen)tre 00 001012990073 0o10l29i07 o002i86) 0o10i28s43 otomis
NSumebter soos 0o33m0o0o1m8 oonoooms 0O0B8 ooowmn osm
ExtDraucetion sooo
AnDaalytseis aur
35o0o0n1 4anaon1 aeoonn aaamn 3a0o0l 4a2p0m1 3w0o0m aaaann wom AVwERmAGE: STANDARDDEVIATION:
FoAunnadlypthe ) No oNsQs aassee ulsaen Aesee eNsQo NG
NQ = NotQuantif(ieisstbhaentheconcentration oftheloweststandard(0.1 ng/mL) LOQ=10ppb
** Possible contaminationbyyolkoroutershellcompoonfseamnpltesinceoutershell
`Rweapsrensoetnwtaatsihveedrpeqruiesottrotsheepearvaatliuoenosfweirnetenmoatlucsoemdptoonceanltcsu.latAesthpeeravSeproangseoarnd standard deviation.
CenteAnalyticalLaboratories, nc.
Page201102
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000285
I--
Centre Stady No 023.063
Table XLIV. Summaryof PFOS for Week 6 Quail Albumen Control Samples
Sponnsor CatDes BBoLroosSsAzonrSs lOosmoos BEooLsrssomts 0011002990088 mBioisosasmr oowwsisz EBiousssamsor oollzessss oELoplspasmsee_oi0o0sin6s
NaSmebee EmDuien ADmuiisk FouAnmdipeb)
wmoaa 30000 mwmnl
No xo
mmuooan cceeno aanoonn xxoo
oowxoeAn aceoo aanoonn
x no
ooxxeon cooooor aannoonn xxoo
ooxxeon ewona aon Nxoo
STANDARDDEAVIVAETRIAOGNE: ~~ NNQQ
Table XLV. SummaryofPFOS for Week 6 Quail Albumen Samples at 62 ppmal
SponDsor CatDre BToLssTa 0o0l BBlolossssaomem 0o0l2e9s BBiLmSsaTmUs o01s02o90 ELoLseSrTs oOlo0Sm BEoLooswsTeis 0o10s24e8 *Duplcat necion
NoSmaber BmDcuskn ADmaitek FouAnmdiiep) Goooam aanonni waimr S5334 mmoolna e0n aannnn 6as1t9 oGxxeonA oaono samnnn osnn cGmHoAn a30m0 mainn Ssi2s6 GN A aT 0 am 3a536
STANDARDDEAVIVAETRIAOGNE: 518122
NQ = Not Quantifible (less tha the concentrationofthe lowest standard (0.1 ng/mL)
LOQ= 10 ppb
Centre AnalyticalLaboratories, Inc.
Pa 44g of1e 02.
000286
Centre Study Nos 023-063
TableXLVI SummoafPrFOyS forWeek
17.6 ppmai
Sponsor
Centre
su
m
IY Number
BEoLO02s5L207T47 00110022995522 0ox00iA
oELOsLs2aS0o2n0aMgSe 00110022995566 ooxxoollaa
EOELDLOSO02704M9s 00110022996600 oomnao
EEOOLLO22SS9S220077SS00 00110022996644 oopowoliA
EEDOLLOs299220m7SSL1 00110022996688 oonnao
6 Quail AlbumenSamples at
Extracto Amipds Date Daic swaooonr uawoonr woowo aaiioo owoowl waoirm wwooowl awomn wwoern waoun STANDARDDEVAIVAETRIAOGNE::
Amite Found (ppb)
9o8s1 11321 11426 116637 i1s5s6 21343
`TableXLVIL Summaryof PFOSfor Week 6 Quail YolkControl
Samples
Sponsor m
Centre
set Number
Ecracion Date
Amaipds Amiyie Date Found (ppt)
EEOOLLO22ss2520077535e 00110022990011 oonoon wwomml aalwan NNeQ
EELOLOS2SS0207766 00110022990055 oowusosB wwomm laaawnl NNQo
EBOLOO2SLS07TSIe 00110022990099 oosooo o9mm IImaanwmmLn isisee
EOL2020 0102861 EOLO2SS0I0 0l0284L
ooo caso
wml aml
a Iaam
NNQo
EELO2Ls22o00mT88 00110022991133 ccouuis wnmolm a iamm NNpD
* Duplicate nection
STANDARDDEVAIVAETRIAOGNE::
NQ NO
INDN=Q=NNootpQeaukadenteictefd(ilessstbhalntheconcentrationofthe loweststandard(0.1 ng/mL) L0Q= 10 ppb
`+w+aPsohsesdibprlieocrotnotasmeipanraattiioonn bofyionutteemraslheclolmcpoonemntsp.oAonsfspeaemrnpSlpteonssionrceRoeuptreersesnhtealltiwvaes not request these. values were not used to calculate the average and standard deviation.
`CentreAnalytical Laboratories, Ic.
Pagedsof 102
000287
---- ee ---- -- -------------- ----------
Centre Study No: 023.063
Table XLVIIL Summary of PFOS for Week 6 Quail Yolk Samples at 62 ppm ai
Spoor Cenmtre B EOLoL s920S702 oOlIsE EEOlLOmNOa0MrS oolloessn BEoLomsloaamasse 0ol0o2993s7 EEoOLLmOsNsOa2m0eAsSe o0lioassel EEolLOm2sSOa2o0uM6se __oowln02soeess
NomSbeer ExDruceton ADuubee FoAunsdipeh) oomsOoD waoomr aunnaomne s0o,r oomoonp wweee wwomm i 30g oooop wwomm wwoamn 22s0 ooowp wwomm wmoamm sooo oGoopD wuowm wwoomm oosewo.r
STANDARD DEAVVIEARTAIGOEN:: 373611000
Table IL. Summary of PFOS for Week 6 Quail Yolk Samples at 17.6
ppm ai
SponDsor BBOLomSaO2oAT BEOoLOmSS2a0UrS EOLONO20US EBOLoOSlO20a74s9% BEOOLLDOSSOO22S00ST EoLos9amste *Duplicate jection
Care 001020945 0o1l02s95)3 olomssT o01w0a2s9s5t7 01l002295661S _ onoasss
NugSbter oooupi oowooD owoD wosno oooop ops
ExiDruceion ADmuiee wwmmll wwnnanan wwomm wwaann wm wom meomm aanamm wwomm wwoaon wom AsVEiRaAnGE: STANDARD DEVIATION:
FoAunmdipteh) sswceo, eosos' so, 4e6e50,0 sso e 5s65o00 6510
NQ = Not Quantifiable (less than the concentrationofthe lowest standard (0.1 ng/mL))
CentreAnalyticalLaboratories nc.
Pagesot 102
0600288
-------- ee ---- -------------- --
Centre Study No.: 023-063
TableL. SummaryofMoisture DeterminationsforMallard Membrane Samples
Sponsor Eo0L100226633.2200885523 EEooLaas63220088842 EEooLLa622s3028082053 EF0Lo0r26m3s:22008m462 EEooLL00226633.2200884478 EEooLL2a62s332.028085506 EEo0L1a6s32200885571 EE001102G6a3s:s20089598 EEO011..00226633..2200009945 EOB1oLL00226633..2200907957 EE00LL0022863:32210090719 EE0011..00226633::2200997818 EB0o1.r0a26s3:2200909819 EEoLoar2a6s3s.2210009002 EEoorraa2zss3s2211000034
Cennte [ourosrans o0w103e208 oowsoisns ooaris ooiossisss oaiooss2i1s6z oai0omsz9z60 oouoomsss olo03o7m68 oouos 0o1l003779068 oolo0s7earss oollooresse 0o1i0o3sr8s0z0 oouwosassooes
MAvoelrsnugres s#s000 70500 0100 71500 a@s0o 7s5000 51500 715500 14 0s0o 07500 0750 00 71500 1500 sso
CenteAnalyticalLaboratories, Inc.
Page70f102
--_me --mmm
Centre Study No.: 023-063
Table LL. Summary of Moisture Determinations for Mallard Albumen Samples
Spoor maEoLssse208r53
Eo0r1c2a8s3220085842 BBoL0c2ass3s2200884235 EEOo10n28s3.220008426 EE0o1L0a226633..2200886478 E0ELo0v2a623s:32200885560. EBooLaraa6s3200885571 EOEO0L100226633..2200895983 EE001L02266332.02909959 EEooLL2286332200099775 BEoorL2sss32210000719 EB0o1l 285332200097818 E0ELo02c6a3s:s2200908019 EE00LL00226633.2210000920 EBool s83211000080
Centre I") mm olosns ooluosine 0ou00ma16ms parseit oowsss oowusi ouolsazsias1n ooul0osszezss ooisem oowuossess oousso oolwseesn oprleed ou01s0s3705r3 poeaeses
Aversgs % Mobture %0800 --
8050 2#00 11600 00 885500 5u0o 88500 155500 2s0o usoo 8o0o 8040 B86o0 m2o0
CentreAnalyticalLaboratories nc
Page48of102
0600290
EE
Centre Study No: 023.063
`Table LIL. SSaummpmlaersyofMoisture Determinations for Mallard Yolk
Sponsor TT E0E1r263o.208s53
E0EL0100623823020084524 EE0oL0L2m6e3:y2200804235 Eo01r6s322008m425 EB0010128633.2200884487 EE00L10.2026633..2200885560 EOE1o.0v226633.2200888571 BEooLL226633220089538 EBooLv2e63y2200099954 EE0011 0228633.220000797 E0BoLLaa226s332201000719 EBoOtL.00226s3.32200097818 EEoorL02a66332200909819 EBOLo02n63s.2201909002 E0BLo26v3e2211000030
CenDtre ow003s202
o010s32s06 ooucna0em ooanrss ooassiss ofornen oowsss oowmne oowesso olossesn ootaasse7s0s oowwnsers oowtaonsss 0o1u0o3s75s0s ooltosss0s
MAovtersatguers wsoo -- awsoo 00 a00 wao0 s000 w0o 050 w0o 060 5s0o wsoo sss000 2S100 uuos
Centre Analytical Laboratories, oc.
Page avr 102
000291
_--_--
Centre Study No.: 023-063
`Table
LIL
Summary Samples
of
Moisture
Determinations
for
Quail
Membrane
--_--Spomnnsor
oCamnre
e%AMMvoeolrsstgwueree
EB001100225599:-2200%50070
o[wesn
5ss0o
E0ELo0r2e5s9p2200753286
oloains
swoo
EBoOLa022s53s2.0270425
porlea
50
--EnoEEosoLaazosspos2a007e4s80
ooowuommnsesss
e3ms0
`tThheerepewracsenntotmoeinsotuugrhe wsaamspdleeteavramiilnaebdleffoorrcitghhetootuhterosfatmhpel3es0.samples received because
CentreAnalyticalLaboratories nc.
Page500f102
000292
_--_--
Cente Stady No: 023.06
Table
LIV.
Summaryof Samples
Moisture
Determinations
for
Quail
Albumen
--_-- Sponnsor
oCenmtnre
sMMAovelesratwgueree
Eo0L10022559-.2200550067
Goilooiissessz
00
EEoorr2sspn0250201
oooamses
0880
EE0oL10025295-52200552069
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