Document YGovQv1dnR25aZrX1k21RGozV

acoarpt fro. latter vrittan bT Dr* * Mastromatteo, Division of Industrial Sy- giene, Departent of Health. Pro^nea of Ontario, dated Octobar 13, 1959, ra Vinyl Chloride ax?erla.nta >n ,mI1 ....... ................................................... __ y On April lit, 1959, group* of nilo ccnpriring 5 nioa, 5 rata and 5 guinea Plea vara exposed to Tarries ao?ntrttloiM ? rn7l chlorida in air for a period of 30 minutes. Hie exposiT** **** conducted in a special animal chamber. Concentrations of 10, 20 and 30 pi*** 9{ vinyl chlorida in air vara used. An addandua to this lattar vill describe k" ** aoneantratione vara ealculatad. An additional group of 5 gninee piga va? exposed to a cone .stration of UOf vinyl chlorida in air on tha u. data. In alii Urintal animals vara involved, including 1? oontrol animals. 9m control T?1-*1* apprised $ rdom, $ rata and 5 guinaa piga, and thaaa oontrol aaimls caaa^?* nil **ocko and vara fad and housed in tha aam mnnar aa tha otJ?r ***! A suwaary of the reeults obtainad ia given balavi Tbn Par Cant Vinyl Chloride in Air Mica Rats All vara lying down at tha a-'V>f-30 irf/rU ahovlnS tremors and twitching) raoovary wJ ^Jfin 1 *irj no deaths. All vara lying still at tAe ~nd of tha experiment) raoovary vas quick in fresh air) no daetha. Guinaa Pigs Ona %.* in side position) four vsra standing) recovery vas quick) no deaths. Bienty Per Cent Vinyl Chlorida in Air Mica On# mouse vas dead) four vara unconscious) raoovary in fraah air took about tan minutes* Rats All unconscious vith shallow, rapid res. ira tions; recovery took about ten minutes in fresh air) no deaths. Guinea All vara in sida position) raoovary in fresh air took about Piga 20 almrttsj no daaths, Ihlrty Par Cant Vinyl frilorlda in Air Mica All fir. .lea daad,* Rata All five rata daad. Oulnaa Piga All fira vara alira but unoonscioua vith Mallear rapid raapiration. Ona guinaa pig fro* this group diad after 2k hours. Forty Per Cent Vinyl Chlorida in Air Guinaa Piga Only guinea pigs vara exposed. Ona vas daad ard four vetr* unoonacious at the end of tha experiment. Anothar one diad vithin 2li hours after exposure. Recovery after exposure to fresh air in those which survived vas slow, BFG3761-1 Page 001 BFG03761 uCC manual.max -ccarpt continued post Morten Examination All animals which died as a result of exposure to vinyl chloride on April lhth, 1559, were dissected within two hours. I carried out these dissections and I made observetions on the gross changes present* Various tissues were removed and preserved in fire per cent formalin for later detailed micros copic study. The two guinea pigs showing delayed death were likewise dissected the following day. All exposed animals which survived were killed os April 27, 1955 (13 days after they were eaposed to vinyl chloride), I performed dissections on all of these and tissue specimens were also taken and preserved in five per cent formalin. Control animals from the same animal stocks and housed under similar laboratory conditions were killed in the same manner and post mortem examinations oondueted in the same way. There was, of oourse, no exposure to vinyl chloride with this group. In general, all animals ihidb died as a result of exposure to vinyl chloride shoved congestion and/or haemorrhage of the lungs and congestion of the liver and spleen. The control animals did not show these changes, I transported the tissues preserved in formalin to Dr. H. Dancigar of Welland. Dr. Danziger had agreed to prepare these sections for microscopic study, and to examine them. lours very truly, (Signsd) E. Kastromatteo, M.D., Division of Industrial Hygiene Hllllillllilil BFG3761-2 Page 002 uCC manual.max tt es 2 3 BFG03762 V APPSNDU Concentrations of vinyl chloride gas mixed with air, ranging fro* $% to hot tinyl chloride, vsts delivered to a $6-lter capacity animal taat chamber* Vinyl chloride gaa and air vara delivered through a rubber tuba to the animal chamber. Eli* tube bad a glaaa Vwith connection* to 1) a "Fisher* flow no tar and the Tinyl chloride supply tank, and 2) a Precision* vat-test gaa mater and standard air puap, lb# flov rate of vinyl chloride was ad justed by means of a valv* os the supply tank and of the air by adjusting the panp rate* Fresh air and vinyl chloride in the desired proportions ware obtained by Vthe flov pool through the tube and rubber tube fro* die measured and controlled sources. Ihe combined rat* of flow for the 5% to 2C concen trations v*s 12 liter* par min* and for tha 30 and U0 concentrations the rate was 8 and 6 liters per minute respectively. coozcsoz BFG3761-3 Page 003 uCC manual.max BFG03764