Document YGJ9jm1bmwwnRDRrRo1ng3Znk

BIODEGRADATION TEST SUBSTANCE Identity:Perfluorooctanesulfonylamido(ethyl)acetmaatye;also be referredto as PFOSAA, FC-128 or as the major component of FC-1 27, FC-129, FC-1 09, FC-1 09-X or Rl 904. (Glycine,Nethyl-N-[(heptadecafluorooctyl)sulfopnoytla]s-s,ium salt,CAS 2991-51-7) Remarks: The testsample isFC-129 referredtoby the testlaboratory as R1904. Currentinformationindicatesitisa mixtureof42% test substance,32% water,14% 2-butoxyethanol4,% ethanol,and 8% C4-C7 fluoroalkyclarboxylatecompounds. Test materialwas from 3M production lot930014, mfg. date 3/97. The followingsummary isabbreviated because the testsample isa mixture of the testsubstance in a waterlorganic solvent solution. Data does not accurately relatebiodegradation of the testsample with thatof the testsubstance. METHOD: Method: Not noted. Type: Aerobic GLP: No Year completed: 1997 Contact time:28 days lnoculum: Polyseed NX, lotD257 RESULTS COD: BOD 462,000 mg Day 5 10 20 28 02 / kg chemical FirstReplicateBOD Mean Value (mglkg) <39,800 186,900 82,700 289,000 Second ReplicateBOD Mean Value (mglkg) <39,800 156,750 179,400 289,000 Remarks: The BOD and COD valuesare expected to be the resultof biodegradationofthe organicsolventcomponents ofthe testsample. These values are notexpected to be representativoefthe Perfluorooctanesulfonylamido(ethyl)acetate. CONCLUSIONS This testingindicatedthatsome portionofthe testsample biodegraded in aerobicenvironments capable ofsupportingbiodegradation. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 DATA QUALITY Reliability:Klimischranking3. This study lacksinformationon the methodology. Testingcannot concludewhat portionsofthe testsample were responsibleforthe apparentbiodegradationvalues. Comparison withthe COD value fordeterminationofthe levelofbiodegradationisnot valuablewithouta descriptioonf how the COD valuewas obtained. REFERENCES Thisstudy was conducted atASci Corporationof Duluth,Minnesota atthe requestofthe 3M Company. OTHER Last changed: 5/17/00 AScl Corpomtion/AScl-Duluth EnvironmentalTestingDivision Submittedto 3M Company - 7/97 ASel-DETD Study ID #5010-063 R1904 BOD REPORT FOR 3M COMPANY Submittedto 3M EnvironmentalTechnologyand SafetyServices 935 Bush Avenue Building2-3E-09 PO Box 33331 St.Paul,MN 55133-3331 Preparedby ASci Corporafion/ASci-Duluth EnvironmentalTestingDivision 4444 AirparkBoulevard Duluth,Minnesota 55811 Sponsor: 3M Company Sponsor Study (D# R1904 JULY 1997 COD = 462,000mg 02 / kg chemical. Datesof BOD test:6/11/97- 7/9/97. Water bath temperaturerangeduringthetest:19.0- 20.OOC. Lot number of PolyseedNX: D257. TABLE 1. BOD DilutionInformationfor R 1904 suvie ID (mg/L) 10 is 25 so Swck Solution Conwntmtion (mg/L) 1005.04 1005.04 1005.04 1005.04 Sample Volum (Ud) 3.0 5.5 7.5 15.0 ASel Corpomti-,-JASci-Duluth EnvirorunentaTlestingDivision Submittedto 3M Company - 7/97 ASel-DEM StudyM #50104XB Smple Con=tmtion (mg/L) 10.1 18.4 25.1 50.3 TABLE 2. BOD Blank CalculationsforR1904 Sample ID Blank Blank Blank Blank Day 5 10 @ 20 20 2125808] FIRST REPLICATE InitiaDl.O. FinalD.O. D.O. Drop 8.89 8.89 8.39 8.99 8.90 8.79 8.52 8.40 -0.01 0.11 0.37 0.49 SECOND REPLICATE InitiaDl.O. FinalD.O. D.O. Drop 8.84 8.94 0 8.94 9.64 0.20 8.99 8.50 0.34 9.94 9.52 0.32 Sponsor: 3M Company Sponsor Study M# R1904 TABLE3. ASCI Corpomtion/AScl-DuluthEnvironmental Testing Division Submitted to 3M Company - 7/97 ASCI-DEM Study ID #5010-M BOD Seed CorrectionFactorCalculationsfor R1904 Se,cdConc. Day MVBOtLic 10 5 10 10 10 20 28 10 FIRST REPLICATE Initial D.O. Final D.O. D.O. Drop 8.90 6.01 8.90 5.87 8.90 8.90 1 4.46 3.52 2.89 3.03 4.44 5.38 SECOND REPLICATE SCFA fi" Final D.O. D.O. 0.558@8.91 9..901. --5.63 0.61 8.91 6.02 0.89 8.91 4.05 D.O. Drop 3.29 2.89 4.85 11 8.91 3.56 '5.35 a SCF = (D.O.drop* nd swd intest)n-fsleed in seed blmik. Average SCF SCF 0.66' 0.58 0.97 I.LJ 0.62 0.60 0.93 TABLE 4. BOD Reference8 and Tox Blanks Calculationsfor R 1904 Conc. Day SCF mg/L 0 50.3 5 1 0.62 5=0-62 FIRST REPLICATE Initial Final D.O. D.O. D.O. Drop Corrected Drop 8.90 4.90 4.72 4.10 8.91 2.34 6.57 5.95 BOD mgllb 205 298 SECOND REPLICATE Initial Final D.O. D.O. D.O. Drop Corrected Drop 8.89 4.64 4.25 3.63 8.90 1 3-62 528 BOD mg/L 182 a Reference= 150 mg glucoseand 150 mg glutamicacidin I liteBrOD dilutiownater,testedat2% dilution. b BOD mg/L = (correcteDd.O. drop) 300 ml BOD volume / 6 nilreference. Sponsor: 3M Company Sponsor Study ID# R1904 . -2- AScl Co.-p--raC-on/ASci-Duluth EnvironmentalTestingDivision SubfnittMto 3M Company - 7197 AScl-DEM StudyID #5010-M TABLE 5. BOD CalculationfsorR1904 Cone, Day SCF mgtl 10.1 5 0.62 18.4 5 0.62 25.1 5 0.62 0.62 FIRST REPLICATE initial FuuLl D.O. D.O. D.O. Drop Correcte d Drop BOD mg&e 8.90 8.00 0.90 8.89 7.96 0.93 8.90 7.74 1.16 9.88 6.61 2.27 0.29 0.31 0.54 1.65 32,&W Mean <39,800 SECOND REPLICATE Initial FuW D.O. D.O. D.O. Corrected Drop Drop BOD mgfkg 8.91 9.32 0.59 9.90 7.83 1.07 8.90 7.72 1.18 9.90 6.70 2.20 -0.03 0.45 0.56 - 1.58 31,400 Mean <39,800 10.1 1.0 0.60 18.4 10 0.60 25.1 10 0.60 0.60 ploo 10 9.90 6.29 2.62 8.89 7.20 1.69 8.90 2.74 6.16 9.99 1.31 1 7.57 10.1 20 0.93 18.4 20 0.93 25.1 20 0.93 50.3 20 0.93 8.90 7.02 1.88 8.99 6.00 2.89 8.90 5.69 3.21 9.99 4.20 4.69 2.02 200,000 1.09 - 5.56 222,000 6.97 1 138,600 Mean 186,900 9.91 8.90 8.90 8.90 7.60 6.02 3.54 0.05 1.31 2.89 5.36 8.85 0.71 - 2.28 123,900 4.76 189,600 8.25 1 - Mean 15667650,d07o50 0.9s - 1.96 - 2.28 90,800 3.75 1 74,600 Mean 82,700 8.91 7.28 1.63 8.90 6.04 2.86 8.90 1.48 7.42 8.90 1 2.95 A 5.95 1 0.70 - 1.93 - 6.49 258,600 5.02 1 100,200 Mean 179,400 10.1 18.4 25.1 50.3 28 1.09 28 1.08 28 1.08 29 1 1.09 9.90 4.96 8.89 2.40 8.90 0.48 9.88 1 o 3.94 6.49 9.42 2.96 283,000 8.91 5.06 3.85 5.41 294,000 8.90 2.25 6.65 7.34 - 8.90 0.12 9.78 8.90 0 Mean 289,000 2.77 274,000 5.57 303,000 7.70 - Mean 299,000 BOD = correcteddrop x 1,000,000 testconcentration. Omitted data g!oints: D.O. drop correctedfor SCF too low Final D.O. < 0.50 mg/L. 2.0). Sponsor: 3M Company Sponsor Study ID# R1904 -3- ASel Corpomtion/ASel-L-Juj'uth EnvironmentalTestingDivision Submittedto3M Company - 7/97 ASCI-DETD Study M #5010-068 APPENDIX A Raw Data Sheets Sponsor. 3M Company Sponsor Study ID# R1904