Document YGDpGnXbkZgZn6BO7QJdR1arn

BIODEGRADATION TEST SUBSTANCE Identity: Nre-fmeertrheydltpoerafsluNo-rMooecFtOanSeEsAullcfoohnoalm,idFoCe-t7h9a0n,olo;r mFaM-y3a9l2s5o.be(1Octanesulfonamide, N-methyi-1,1,2,2.3.34,4,5,5,6,6,7,7,8.8,8heptadecafluoro-N-(2-hydroxyethyl)-, CAS # 24448-09-7) Remarks: Material number is an were off-white, waxy not recorded. solid. Sample purity and lot METHOD Method: protocols COD/BOD study using 3M Environmental Laboratory standard modeled after the Standard Methods for the Examination of Water 1976. and Wastewater, 14" Edition, American Public Health Association, Type: GLP: Potassium No dichromate reflux COD and Aerobic BOD Year completed: 1979 CInoonctualcutmt:imNeot(unnoittesd).: See Remarks dRueematrokisns:ufTfhiceielnatbooxraytgoerny wdeapslentoitona.ble to generate useable data for BOD The COD was done in replicate. RESULTS COD: Analysis 1 = 170,000 mg/kg Analysis 2 = 156,000 mg/kg Average = 163,000 mg/kg sBuOsDp:enAdetwdesnutbys-tdraaytebicoocnhceemnitcraaltiooxnysguepntdoe1m0a0n0dm(gB/OLDs)hotewsetdwiitnhsufficient oxygen depletion in excess of the blanks to allow the calculation of BOD dvaelgureasd.eTihnias nreosnu-latcicnldiimcaatteeds taheartobNi-cMbeioFlOogSiEcalaltcroehaotlmeisntnostyslitkeemly. to Remarks: A 259 mg/L potassium hydrogen phthalate control, running cmoenacunrrBeOntDlyfowritthhitshceonstaromlessuebsetdrawtaesatfotuhinsdctooncheanvteraatiBoOnDi;s o2f0823m1g./L.The This indicates that the seed was active and acceptable for BOD studies. 006521 TcuhletufraecstctohnattaoixnyinggenN-deMpeleFtOiSonEraaltceoshdoulrcilnogstelhye ffiorlstlofwoeudrtteheonsdeaoyfsthien. seeded controls 1o heterotrophic suggests that the microorganisms. lack of degradation is not due to toxicity DATA QUALITY sRueflfiiacbiielnittlyy:cKhiairmaicstcehrirzaendkianngd=th3.e iTnhoecupluurmitycoonfcetnhtertaetsitosnuibssntoatncdeesicsrniobted tthhaotugehxcteheedceodntwraotleirndsiolcuabtielsitiyt,wtahsusactthievet.estStsuudbystuasnecde iwnadisvindoutalsowleuibgihlitzsed and was not available to microbes. REFERENCES 3ReMinTeerc,hnPirocjaelctRe8p9o7rt0s0:12"6B0i0odPergermadaantuifoanctSutruidnigesNootnicFeMFC3-82759"0,, E.A. Report No. 016, January 8, 1979. "Chemical Oxygen Demand of 9970012600 Premanufacturing FM 3825", E.A. Notice FC-790, Reiner, Report Project No. 017, January 8,1979. OTHER Submitter: 3M Company, St. Paul, Minnesota, 55133 Environmental Laboratory, P.O. Box 33331, Last changed: 5/17/00 006522 | rrForm eons 874711:4 omni ee or TECHNICAL REPORT SUMMARY (tmportant ~Ifreportisprintedonbothsidesof paper,sendtwocopiestoTCC.) "1/8/79 i | Environmental Laboratory (EE & PC) 0535 Prenanutacturing Notice FC.790 So70012600 Blodogradation Studtes on FH 3025 V. Pothapragada EA. meiner ra1e TYNomsE-70S0ee cEontvaironmerBntealaLamb Reque|stA"#R41E9E7 rToar ane awe ESE | 10 evaluate the susceptibility of FU 3925 to B BFiuosdreogcE rhaodmaitciaoln biodegradation. P5egeaoation B A e e en n sSSAhuolstnpweeeednntdyeiT-dondsausMyfuifbiisboctniiroeactnthhteeemoiccxcaoyalneliceuenlnoaxttyrdigaeoetpnniloetnodisfeomnuBapOnDdinto(voBaOrl1Duc0)ea0sg0:stemsgoft"/.Tlhwtiihsteh I TestsRoninadcieclaIvmetsednabriolFoigi3c9s5]5 tisrenaottmenLtikeslyystetmo: degrade PFTilloold3ee4g2r2Tahdheaanssiieoapntrtoyvsvieosnuisnli1y3labrneceetlnoinBfaiotuenod4d25ts,oerboPebNicr59os5s5iasttiaeonntp.rotobuSsinlcye ASsiotree!aintent Yo blodegredation 1a' see nated microbial a) eal 006523 ao BIODEGRADATION STUDIES ON FM 3925 Introduction PCriezvOio)u,s shtauvediesshoownn FtMhat342t2h,isetchoymlpoufnodse1salcnoohtolmea(sCigrFd,f.i,ySOf,eNg(rCiyaHd)a~ Tbjheredfitohreer, acitcliampapteeardedorunnloimkaeclcylimthaattedtmheixeFdM 3m9i3c5r,obimaelthyclultfuossees (1,2). waoluclodholbe (rC8Fa.dllSyO',dNe(grCfHi,a)aCB,lH8O.R),Thebecfaoulsleowoifngiwtosrkchweamsicaclonsdiumcitleadrittoy, check the susceptibility of FM 3925 to biodegradation. Ultimate Biochemical Oxygen Demand FAM3tw9e2n5t.ya-cdcaoyrdbiinogchetmoictahle osxtyagnednarddeEmnanvdiro(nBmODe)ntawlasLacboonrdautcotredy opnrotocol s(oaltitadchfeodr)m, aenxdcetphtat tthhaet bFoMtt3l9e2s5 wcaosntpalianciendg FinM t3h9e25BwOeDrebotctolnetsinuionusly satHiarcrhedMofdoerlt2he173enwtiitrhe miangcnuebtaiticonstpierrriiondg.barSst.irriThneg wsaesededodneconutsriongls and reference compounds were not stirred. TTFhhieegurmreeestuh1l.otds "rNoeofqubitirhoiedssegsrttahudadatytitohanereodsfihsosFwMonlv3ei9nd25ToAxwBayLsgEenob1 sdaeenrpdvlaebtarlieeonpblyionttthaeidstesimntethod. cleualsttur2e emgx/cle.ed Tthhiesoxdyigdennotdepolcceutri.on oTfhusthetheseBeOdeDdcacnonnottroblebycalactulated. 2Th0e00BOmDg oatf oexaycghentipmeeriksgtohferetfhoirsecocmopnosuindde.red to be less than wAit2h50thmeg/lsapmoetasseseiduwmashydfroougnedntpohthhavaelat=eBOcDogntroofl23r1u.n TchoencumrearnentBlOyD fionrdictahtiess ctohnattrolthesubsseterdatweasatanthaicstivceoncaecnctepattaibolne siesed208formg/BlO.D This studies. Tlheevelfacitn tthhaits sthteudytesstugsguebssttsrattheatditdhenseotcodmepgoraudnedstwooualddetnoetctadbelgerade significantly in a nonacclimsted waste treatment system. Wchoemtphoeurndorcannnoottmibecrodbeitaelrmpionpeudlaftrioomnsthicsanstaucdcyl.imatHeowetvoerd,egrsaidnec,e this sFhMow3n925notistostrduecgtruardaelliyn vaecrcylismiamtieldarculttourFeMs,34w32e, awnhtiicchipahtaes bteheant Fi 3925 will resist degradation by even acclimated microbial cultures. Tfhoeurtfeaecnt dtahyast oixnygceunltudreepsletcioonntairantiensg F(MFig3u9r2e5 c1)losdeulryingfotlhleowefdirstthose otfo tthoexicsieteydedtocohnettreorlostrospughgiecstmsictrhoaotrgalnaicskmso.f degradation is not due 006524 Fi-gure 1: OBwaytgtolnesCoDnucreinntgraUtiitoinnsocfe 5C0uDlture Linnee Ketoy Study 'or Fe Hos Average Seeded Control A--d 10 66/1 TU 3925 @remnne0 100 mg) FN 5925 Bonne oe . BT 1000 ag/1 BH 3025 eomimk E32 a TI HeinTR ie fs 8Sos s 22 1 2g ss 1 10 34 20 Time (days) & ULTIMATE 80TDaDpAsTA1YOR FM 3925 CoDnesccernitprtaitoinonand Dissolved 0, Concentration bays bas ay 1 Dayo Dwi payzo SSeeecddeedd Seeded CCoonnttrrooll Control ##12 #3 88..99 8.9 sl8.o7 ss 885''.ss4 88s.34s 777..978 655.231 Secded Control #2 8.9 slo 58 sis 81 81 Ave. Seeded Control 8.9 5s ss 5.4 7.0 6.2 Fu 3925: 1301200 mnmegg//)11 858'.s74 8a5..i33s 577..097 777..777 777.334 677...700 1506000 mmgg/sd 8877 88s1 8811 8830 7f8d L7o3se + PPhoithaasisattuee: hydrogen 42.36 mngg/s1 [s5o -: :- -2 =: :- 87'18 amengt 31137 :: :: :: :z :- 31 a8a 5 References: (1) R3%einTeerc,hniE.calA.,ReBpioortdeg8r/a1d2/a7t6i.on Studies of Fluorocarbons, (2) R3eMinTeerc,hniE.calA..ReBpioordteg1r/a9d/a78t.ion STudies of Fluorocarbons-II, EAR/cen 006527 fed - coo. 4197 | + ow! i Fu. oiu i + RULETISMEUAxTPLERETSBSOSEDD ASDmAgTA[iceSHSAEMEPTLE \ | cDoESnCcReInPtTrIOaNTiAoNnD| BODs | BODe |B: oD, {POD,;|BOD |ROD,, nse ! [omg J4 : 529 :i S1060o - Yoo y : : 2 > IWSAUcFruFfIiCeIEBRToo0.0v.al DuEPELsE.TI#ONS [70 conFinfNTLY chcdurare 1 . 1 LESS THAN VALUE + I i for + + + + <lpoo A{LorYes FWuEsRaEzPs ACEwasD`.ApOiaNce C|OiNFINmUBiSe SsToIfRRImNoGrrWiIsT| m(RACHeMOsDEoLoZT[Er)ormW.imHme|sMoArGNmETeIsC HHEiLL SeTIaRRnING BARS. THE SAMPLES . WERE[ Mixed ouRiNG THE ENTIRE INcusTion HEE a od tp !H .ad0.D= afw/a Ky piid ; oh 75% 000 Il AT ae es,000 r SSg tLH+ bls 88 ia idl: ~ ; "~ Fomanria TECHNICAL REPORT SUMMARY TO: TECHNICAL COMMUNICATIONS CENTER -- 201-26N (importan=t I reportsprinted on bo siodfpaepesr, sntcw copietso TCC.) "1/8/79 war Erivironmental Laboratory (EE & PC)% Premanufacturing Notice FC-790 Chemical Oxygen Demand of FM 3925 V. Pothapragada i05t3a5 9970012600 017 E. A. Reiner 47816 SECORITYD NoLnOe ,- CSoenetinE)nvirBTooncimomeSsnnotraialnioLna) b Request #4197 | "ECoG a amorRee LES ronos:eman CoRRENT GRIEGTIVE 1 DumiSmmorr | To determine the extent to which FM 3925 is oxidized by CHomical oxygaen | SrTeTfolmuExliyngaciinditchecponrdeisteinocnes.of potassium dichromate under Demand (COD) Degradation r Fluoe roche emical [Eran AeSTRoACeT e07R0RvRereh)eThymSitnimamtoieon vey Tei Commniatar Cor The chemical oxygen demand (COD) of FM 3925 was determined according to the 3M Environmental Laboratory's standard pprroocteodcuorle.whiTchheisCOmDodweasleddetaefrtmerinetdheto1Sbta6end3ard,M0et0hmog0d/skg. This is 35% of the total theoretical oxygen demand c(aalscsuulmaitnegdNasissumniotngoxtihdatizeodn)ly atnhde 7n1o%nfolfuotrhienaotxeydgenpordteimoannd of the molecule is degraded. REL) 006529 "2- CHEMICAL OXYGEN DEMAND OF FM 3925 Tthoe thcehesmticaanldaorxdypgreontodcemoakndofoftheFMEn3v9i2r5onwmasentcaallcLualbaotreadtoarcycor(daitntgached). These protocols are modeled after the procedure described in Standard Methods (1). `The results of two replicate analyses are shown in TABLE 1. . OD TofABLFEi 13925 Analysis 1 170,000 mg/kg " Analysis 2 156,000 mg/kg 1 Average 163,000 mg/kg This COD is 85% of the total theoretical oxygen demand assuming cthoemploextyegenoxiddeamtainodn catolcuFl_,atCe0d,'ass8u0f,i,inNgH,T,hatanodnlH,0.the Itnonisflu7o1%rinofated portion of the molecule is degraded as in the following reaction: CyT17S0aN(CHZIC0oH + 4 057 CgFy7S05N, + 3 CO, + 3 Hy0 om 557) Reference: (1) Standard Methods for the Examination of Water and Wastewater, 14th Edition, American Public Health Association, 1976. EAR/cen Attachment 006530 3M_ENVIRONMENTAL. LABORATORY C(HUEsMeIdCpArLioOrXYGtEoNJDaEnMuAaNrDy P4,ROT1O9C7O9)L EAR 1/4/79 mCheetmhiocdaldeosxcyrgiebneddienmanSdtanisdarddetMeertmhiondesd bfyoratmheodiEfxiacmaitniaotnionofofthe 5Wa5t4e,r(an1d 0Wa7st8ew)ate.r, American Public Health Assocation, p. 550- ProcedureModi fications : MTehtehordesfluixn tahpaptara5t00usmldifrfoeurnsd-bfortotmomtehdatbodielsicnrgibefdlasinksSwtiatnhdargdroundgErllaensmse2y4e/r40 flnaesckks.are Inuseadddiitniopnl,aceeleofctrciocmpahreaabtlieng gmraonutnlde-sg,lawsshich farleaskcsa,pabalree uofsedadeinquaptlealcye boofilainhgottphelatceo.ntents of the refiux o1 pThreiorsiltvoerresfullufxaitneg cthaetalsyasmtplei-ssualdfduerdicd,iraeccitdl-yditcohreoamcahteCmOiDxtfulraes.k . Tblhaenkf.erroIutsisammaosnsiuummedsutlhaftatemotditircahnrtomaitsestisandraerdduiczeeddinagatihenstblathnek during the reflux procedure. Sample Size and Reagent Quantity and Normalities aSraempltehosseizepreisscr5i0bemld. forThethirseasgeanmtplequasnitzietiiensTaabnldeno5r0m8:a1litoifesthe i1n4t0h.1E2ditgioqn uofaSntatndaainrddtMieIs tehmoodtss,diesxscoelpvtedthiantthAge,'ScOo,ficiesntardadteedd sulfuric acid reagent. Procedure for Low COD Samples iTsheusaeldtewrhneanteCOv SDtaandalrduMoefeth5os0dsmgC/OlD oprrocleedssurehavfeorto1obwe-COdDetesrammipnleeds. tIhnethsiamse,probuctedutrhee nthoermaslaimtpileessoifzethaenddircehagreonmtatequaanntditfieersroursemain armemsopneicutmi'vesluyl.fate solutions are reduced to 0.025 N and 0.01 X, 006531 EAR 1/4/79 CHE3MMICEANLVIORXONYMGEENNTADLEMALANBDORPRAOTTOORCYOL dCahesestmceirwciaabtleedro,xinygeSntanddAemeamricraandndMeitshoPdublicdestHeealfrthomrintehdeAsasEcoxccaoimraidtniiaonntg,iotnpoo otfheWa5m3te0-et55rh4o, dand(1676). Procedure Modifications gTMlheaetshosrde2sf4l/ui4nx0 tanhpaeptcakr5a0at0ruesmuldsiefrdfoeurnisnd-bpfloratoctmeomtoehfdat cboodmieplsaicrnragibblefedlagsikrnsowuSntidat-nhgdlaagrsdreound Efarlralesekncsma,epyaebalrreefulofsasekdasd.ienqupaIltnaeclaeyddboiftoiiolani,nhogteltpehlecattrceio.cntehnetastinofg mtahnetlreesf,iuxwhich Sample Size and Reagent Quantity and Normalities H Snaomrpmlaelitsiiezsesgoifven50inmlStaarnedaursdedMwetihtohdst,heTarbelaegen5t08:q1u.antities and Procedure for Low COD Samples TIinhsetuhsaielsdtepwrhrneoancteedCuOSrDteavntdahalerudessaMmoepftlheo5d0ssmigzCe/OlDaopnrdrocrleeeadsgusernehtavfeqoruatnoltobiwetiCeOdsDetersreammmiapnileneds. athmemonsiamuem, `sbuultfattheesmoolrutmiaolnitiaeres respectively. orfedutcheed dtiochr0o.m0a2t5e N aanndd f0e.r0r1ousN, 006532