Document YGBODdBkvygx18b3MjDgY7MbK

PRIZE 20% 7 CHRONIC TOXICITY TO FRESHWATER INVERTABRATES (Daphnia magna) TESTSUBSTANCE Identity: Perfluorooctanesulfonate; FC-95. (1-Octanesulfonic may acid, a1l,s1o,2b,e2.r3e,f3e,r4r,e4d,5t,o5,a6s.6P,7F.O7,S8.o8r.8- heptadecafluoro-, potassium salt, CAS# 2795-38-3) 3ReMmlaort knsu:mbTehre21l7e.st sSuabmsptlaencweaiss astwohrietdeupnodwedrera.mbiSeanmtpcloendwiatsiontsakperniofrrtoom etelsetmienng.taPluraintayldyesteesrmteicnhendiqtuoesb.e 90.49% by LC/MS, 'H-HMR, '*F-NMR and METHOD Method: 1193-87 OPPTS 850.1300, OECD Guideline 211, and ASTM Standard E Type: Semi-Static Life-Cycle Toxicity GLP: Yes YSpeeacriceosm:pDlaepthendi:aSmtaugdynacompleted 1999. Report completed 2000 SAunpapllyiteirc:alImno-nhiotuosreicnugl:turPeFs,OSWilmdeliafseuIrnetdernoantidonaayls, Ltd., 0, 2, Easton, MD 11, 14, 18, and 21 Exposure period: 21 days Statistical methods: daphnids, the number Survival data of live young was and evaluated the length on first-generation and dry weight of the sFuirsvhierv'isngexfiarcstt-lgesetn.eraRteipornoddaupchtniiodns.anSdurgvriovwatlhd(alteangwtehraenadnadrlyyzweediguhsti)ngdata hwoemroegeevnaeliutayteodf fvoarrnioarnmcaeliutsyinugsiBnargllSehlalp'sirtoes-tW.ilAk'nsaltyessitsaonfdvfaorriance and sDiugnnniefticta'nsttienstcowmapsaruisseodnttooitdheentinfeygattrievaetmceonnttrgorlo(upps= t0h.a0t5)w.erAellssttaattiissttiiccaallly t2e.s0tsorw"eTreOXpSerTfAoTrmReedluesaisneg3a.5p"esrtsaotinsatliccalomspofuttwearrew.ith SPSS/PC Version Test organism Pretreatment: age: < None 24-hours old at est initiation Test conditions Dilution sterilizer twoatreerm:o0v.e45miucmrofoirlgtearnediswmesllawnadtefrinpeapsasrteidcltehsrough a UV iDimlmuetdiioantwealtyerprcehceemdiisntgryth(edutersitn)g: the 4-week period hardness: alkalinity: 112649 ((112604--112782)) mmgg//LL aass CCaaCCOO,3 pH: 82(808.3) Toc <1.0mglL 01035 Conductivity: 329 (315-340) umhos/cm Ca/Mg ratio: 35/135 NalK ratio: 21.3/6.62 Stock and test solution preparation: Primary stock solution was prepared in dilution was dissolved prior water at 46 mg/L. It was to use. After mixing, the stirred until all primary stock test substance solution was. proportionally diluted with UV sterilized dilution water to prepare five additional stock solutions at nominal concentrations of 1.4, 2.9, 87,1, and 23Rmegn/eL.waAlllrattees:t sEovleurtyioMnosnadpapye,arWeeddcnleesardaayndancodloFrrliedsasy.. Exposure vessels: 250-mL plastic beakers containing approximately 200 mL test solution. The depth was approximately 5cm. Number of replicates: 10 Number of test organisms per replicate: 1 Number of concentrations:6 plus a negative control Feeding: Each test chamber was fed 0.3 mL of YCT (a mixture of yeast, Cerophyl, and trout chow at 1800 mg TSS/L) and 0.60 mL of Selenastrum capricornutum (3.5 x 107 cells/mL) once daily. Lighting: Colortone 50 fluorescent lights. Intensity ranged from 329 - 383 lux at the water surface. Photoperiod of 16-hours light, 8- hours dark with a 30-minute transition period. Water chemistry of new and old solutions during thestudy: Dissolved oxygen range (0 - 21 days): 8.3 - 8.9 mg/L (negative control exposure) 8.3-90mglL (12 mglL exposure) 8.4 -8.9mg/L* (48 mg/L exposure) pH range (0 - 21 days) 8.1-84 (negative control exposure) 82-85 (12 mg/L exposure) 8.4 - 8.5" (48 mg/L exposure) Test temperature range (0 - 21 days) 19.4 -20.1C (negative control exposure) 19.4 -20.1C (12 mg/L exposure) 19.4 - 18.5C* (48 mg/L exposure) * (Measurements discontinued at Day 3 due to 100% mortality. Element basis: Survival, reproduction and growth. Effect concentrations based on survival. G1056 Method of calculating mean measured concentrations: arithmetic mean RESULTS oo I Nominal concentrations: Negative control, 1.4, 2.9, 5.7, 11, 23, 46 mg/L Measured concentrations: <LOQ, 1.5, 2.9, 5.6, 12, 24, 48 mglL Element value: 21-day NOEC = 12 mg/L 21-day LOEC = 24 mglL 21-day MATC = 17 mg/L 2" generation acute survival NOEC = 12 mg/L. All element values based on mean measured concentrations Analytical Methodology: Analyses of test solutions were performed at Wildiffe International Ltd. using high performance liquid chromatography with mass spectrometric detection (HPLC/MS). When determining the concentration of the test substance in the lest solutions, the same and most prominent peak attempt was made to response for the lest quantify on the basis substance was used. of individual isomeric No Tcohmepomneeanntsp.rocTehdeurLalOQre(cloivmietroyfoqfuamnattirtiaxtifoornt)ifwiacasti0o.ns45a8namlgy/zLedin this study. concurrently during sample analysis was 104%. Measured values of new `samples ranged from 94 10 121% of nominal. Measured values from the. old solutions ranged from 90 to throughout the renewal periods. 108% of nominal values. PFOS was stable 61637 `Summary of analytical chemistry data: aCr [vvecmo| mee ed ||| a [[Jp amr meo r |[mTee[s]| |bmamgemeneemenan [a] " [ m hm ei|a i+||]wv] NOTE: Mean measured concentrations were determined from new (renewal solutions) and corresponding old solutions during each week of the test. Days 0, 11, and 18 are "new" and days 2, 24, and 21 are "old". Biological observations Satutrevsitvatle:rmAilnlatsiuornv.iviSnugrfviirvstalgeinn etrhaeti2o4nadnadph4n8imdsg/aLppteraeraetmdenntosrmwaals statistically significantly different from the negative control group. Reproduction: Daphnids in the control and treatment groups <12 mg/L started producing neonates on Day 9. The Bonferroni t-test showed that reproduction was not significantly reduced in any treatment group 12 mg/L (p >0.05). The 24 and 48 mg/L. treatment groups were not included in the statistical analysis of the reproduction data due to a statistically significant effect on survival. Growth: The Bonferroni t-test showed that mean length and dry weight in the treatment groups <12 mg/L were not significantly reduced in comparison to the negative control (p > 0.05), Second Generation Acute Exposure: After 48-hours of exposure, survival in the negative control was 95%. Survival in the 1.5, 2.9, 5.6, 0% r1e2s,peacntidve2l4y.mgS/uLrvtirveaaltimnenttheg2r4oumpgs/wLatsre1a0t0m,en1t00g,ro1u00p,w9a0s,. and significantly different from the negative control (p < 0.05). 1053 TSiCuemrnmHaearsaysonno|f| Perocme7nt|Mortaoliwtuy | omar | a o we 0 | 2ss o |o o | Le | u . w T - T 0 w wo |ow fm oT Second Generation Mortali aMietmersed.|| rTnmoasn||| Aetbnnates||| cuF"magiecaorene mTe o Tm] SInudmimviadruyalolfy-LEexnpgotshedanFidrsDtr-yGeWneeirgahttioonfDSauprvhinviidnsg oamm nessieda || sfSaernmeibodar || TotStenm, ia[oyWag,woan +50. [eo | 0[m[o omam m | [se To[asom|oma | [ew To[esom |omeomm| CoLoul Reproduction CMonecan--nt-aotaosnu.rmeod.|| NSDuaupmvtvrbieeggsr||| MoOSoonpLumiivden(YA+ogu5ng)||| R"eFipsetoDduasysoefn|||DTeoat"dNiuNmmmbeoeodrf|||oTAontvaorNeaudmEnxbpear] Nogatwe Conor | 10 1z2at02 so | oo 1s wasn 5 o 2s | ss mens s o | 12.105 o 0 =2 Low ew | 0| eed tone |e |] CONGLYSIONS ee ---- TDhaeprheniwaermeagnnoaadevxeprosseedeffteoctthseolnesstursvuibvaslt,arnecperaotduccotnicoenntorragtrioonwt<h1o2f mg/L for 21 days. Daphnia reduced survival magna exposed to 24 and 48 mg/L had significantly Author and/or submitter: 3M Company, Box 33331, St. Paul, Minnesota, 55133 Environmental Laboratory, P.O. bamquaey 00000000000 Reliability: Klimisch ranking 1 REFERENCES ~~ 00 This study was conducted at request of the 3M Company. Wildife International, Ltd. Easton, MD at the omer 0000000000000 Last changed: 5/3/00 G1800