Document YG0zXRN3wm1eR1mrQ1RBrKXL8

BIOSEARCH.INC. p.o.box 8598 philadelphia,pennsylvania 19101 t o Ia p h o n a 2 1 5 848-4499 Submitted to: Material: Sample Received: Date of Report: Test: Object of Test: Procedure: 3m Company 3M Center St. Paul, Minnesota 55101 3M Company - T-)5u5 August 25, 1976 September 16, 1976 Acute Oral Toxicity Rats To study the acute oral toxicity in rats of the subject material. One group of 5 male & 5 female albino rats of the Sherman-Wistar Strain weighting between 200 and 300 gm were employed in this study. The rats were deprived of food but not water for 24 hours prior to dosing. Each animal was weighed and dosed by direct administration of the experimental material into the stomach by means of a syringe and dosing needle. The sample was dosed as a 50% w/v suspension in water. Results: Conclusion: The following dosage level was administered: 1,000 mg/kg. Following administration the animals were allowed food and water ad libitum for the 14 day observation period during which time the rats were observed for signs of toxicity and mortalities. See Table 1. The subject material when studied in male and female albino rats has an acute oral LD50 of less than 1.000 mg/kg. Karl L. Gabriel, V.M.D., Ph.D. Director Material: TABLE I Acute Oral Toxicity 3M Company - T-1585, as a 50% w/v suspension In water. Dosage Level mg/kg 1,000 1,000 Number of Animals Dosed Mortalities Days 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Total Dead 14 Days 5 males 2 100 000 0 0 0 0 0 0 0 3 5 females 4000000 0 0 0 0 0 0 0 4 The LD Is less than 1,000 mg/kg. 50 The gastric mucosa was eroded. The animals exhibited signs of writhing at times. Males Females InitialWeight gm 24o 220 Final Weight gm 250 220 Total Survi 14 Da 2 1