Document YDn8z9a9QMQ1wnL2keyD140zy

AR226-3005 Haskell liabrslry ReportHo.591-92 tor Economic Co-OpersMpn and Oeve'opment (OECD) Organisation Guideline for Testing Chemicals. 202 Author Kbith B. Pierson, Ph.D fjtudv C July 6 ,1994 E jUtlilL1lafroratea ,^ i ^ SMedidneHask' Elktorl Road, Post O ffiw Box ou Ne wark, Delaware 19714 MedicaLBiSSfiaii tSCACB Does ootco,nVa',n Sal",W'tfed CO'm?an* Page 1 of 15 j i I i DuPont HLR 591-92 TABLE o f c o n t e n t s Page .... 2 Table of Contents.. ....3 General Information .... 5 Summary................ ..... 6 I. Purpose...... ...... 6 II. Records...... 6 III. Materials and Methods.............................................. ........................................6 A. Test Substance........... .................................... .......................................... 6 B. Dilution W ater.............. .......................................... C. Animal Culture.............*.................. D. Test Methods............... ................... a*"""" ......................... 6 .7 .7 E. ' Chemical Analysis......................... ......... 8I I I I l M" F. Statistical Analysis................................... *.......... " ' " .... 8 IV. Results and Discussion. .... 8 V. Conclusion..................... ......8 VI. Literature C ited. . . . . . . . .9 VII. Tables........... v : V Laboratory W elLW ater.................................. 1. Chemical Charactenstics of io h M M in a 48-Hour, i Measured ConcertraBons of H-19518 .. 9 10 Qtatir Un^'rated Test....................... . ..1 1 ......- .................. 15 San" ComPan^ 2 vntSCACBl Docs not co,nta m >4p 'Hf sM -j Haskell Number: Purity Composition: H-19518 C o r 'minante: CAS ReqistoLM^mkSL gponsgf; I { I DuPont Chemicals E. I. du Pont de Nemours and Company Wilmington, Delaware 19898 CSmpany S a n ili *. Does not contain TSCA CB1 j i I r '*.V' i Sanitized. Does not contain TSCA OBI Company 4 DuPont HLR 591-92 STATIC, ACUTli AftHQUR ED50 0 F H' 19518 TODaphnia magna SUMMARY j n hn-a manna nAanates (less than 24 hours old) in an 1-19518 was slightly toxictp Dapnma m a g r w w '* , \ q5, 5.13,12.8, 32.0, inaerated,sialic.-hourW it. 0 .4 5 ,2 .6 7 ,4 .0 7 , 10.0,200, and 500 mg/L. yha 48-hour EC50 based on measured w a s W m g i Mi a95%1iducia, limit o. >2.9 to 98.3 mg/L ........ n ,,. P .L tiJ .P tt * V6P Robed W. Peterson. B. A. Toxicology Associate 6 199 + Mo/Day/Year THIS REPORT IS APPROVER FOR ISSUE. -/L A Keith B. Pierson. Ph.D. Research Toxicologist and Study; Director Environmental Sciences Mo/Day/Year Sanitized. Does not contain TSC A CBI Company 5 DuPont HLR 591 -92 |. PURPOSE The goal o,this study was to assess the acute neonates less than 24 hours old. ||. RECORDS - , * 1 8 to SSToSK'SSlSSKKff"- HI. MATERIALS AND METHODS A. Test Substance The test substance is described t e n k ^ 2 t e k[sSebelowrSncI prepared by dissolving the te^ s^ * ^ k solution were added to filtered fish tanK siirrina for 30 minutes. Aliquots of the st composite was then 1TL orenare H 0 0 mL of composite test soiution i ne c . w a apportioned^inong the fourroplicate aP sealed container with graduated cylinder. Each o> t * * |QSS of , he ,est substance through volatility^ e S a y andato?ub" y T h >95t 8 in filtered fish tank water and storage cond, were not determined. B. Dilution W ater Water ("able 1) to use in this c . Animai Cuiture ^ Parent Daphnia magna re2^ mf (1 oer beaker) which contained 200 mL o S w at Setenastrum capncornutum approximately 20-C. D a p h n e 'ot each spaces (total and Ankistrodesmus ff lcaJ ^ ' Wednescjay and Friday during water renewal (cut 150 000 cells/mL) on Monday, Wednesday Setenastrum atari through day 12) Wednesday. and Fnday S % roagS ^ on the beakers and tes. vesse... Company Sanitized. Does no! contain TSCA CBI 6 were 2.05,5.1 , > DuPont HLR 591-92 j - amaior 12 5-cm solution transitional light (1 to * w Observations were made da y. y ,, springs, OH), the 48-hour test. S ?fe or tour point calibration curve. -a Com pany Sanitized. Does not contain t q ^ a ___ 7 DuPont HLR 591-92 F . Statistical Analysis The 48-hour EC50 was' calculated by the SAS Probh method (21- iv. RESULTS AND DISCUSSION A 48-hour rangerinder weranoa lo" n" .T a n d S100%. respectively. study are reported herein. The definitive study w asJ "n U2 ^ f n 2 05 5 13, in ^ r ^ f ' ^ A n ^ * physical parameters in the definitive tnabUity to swim at least two body lengths 48-hour EC50 based upon (Tables 5 and 6). 95% fiducial interval of 52.9 to 98. J mg/u i,. V. CO NC LUSIO N H-13518 showed slight toxicity to Daphn.a magna neonates (less than 24 hours old) m an unaerated, static, 3-hour test. VI. LITERATURE CITED .,, p ,, ooeration and Development (OECD). Guideline tor 2. Finney. D. J. 1971. Probi. Analysis, 3rd Ed.. Cambndge Un.vers.ty Press 333 p. Company Sanitized. Des not contain TSCACB/ 8 DuPont HLR 591-92 STATIC ACUTE. 48-HOUR EC50 OF H -19518 TO Daphnia magna TABLE 1 Chemical C h a r a d e s of Hashel, Laboratory W eil W ate^ Concentration BOD, mg/L COD, mg/L DOC. mg/L TOC2, mg/L Total Kjeldahl N, mg/L Ammonia N, mg/L Turbidity, NTU Phenolics, mg/L Color, apparent Co/Pt7 Solids Total suspended, mg/L Aluminum, mg/L Antimony, mg/L Arsenic, mg/L Beryllium, mg/L Boron4, mg/L Cadmium4, mg/L Calcium, mg/L Chloride3- mg/L Chromium, mg/L Cobalt, mg/L Copper, mg/L Cyanide, mg/L Iron3, mg/L Fluoride3, mg/L <0.050 <5 a Q0 0060 <0010 < 0 0 01 Q 020 002 3 " ;010 <0.025 q.001 ' Lead, mg/L Magnesium, mg/L MBAS/LAS, mg/L Mercury, mg/L Nickel, mg/L Nitrite3, mg/L Nitrate3, mg/L Phosphate3. mg/L Potassium, mg/L Selenium. mg/L Silver, mg/L Sodium, mg/L Sulfate3, mg/L Sulfide, mg/L Zinc, mg/L Volatile priority pollutants Acid extractable priority pollutants Base/neutral priority pollutants Pesticides/PCBs Organophosphate pesticides5, pg/L <0.075 3.7 <0.05 <0.00020 <0.020 <0.1 1.9 <0.1 1.7 <0.005 <0.010 8.2 5.0 <0.10 <0.058 ND6 ND6 ND6 ND6 <0.50 ,Date of based on cobalt/platinum reference. aetec,ed: 7Unrts Comp, 'anySanfifz^ Ooes ftQt Cnnfa.*. DuPont HLR 591-92 STATIC, ACUTE, 48-HOUR EC50 OF H-19518 TO Daphnia magna TABLE 2 Measured Concentrations of H-195181 in a 48-Hour, Static. Unaeratec rest Nominal Concentration (mg/L) -------- Control 2.05 5.13 12.8 32.0 80.0 200 500 Measured Concentration (mg/L) Start3 0.21 1.99 3.77 12.0 25.5 65.0 162 456 Endb 0.45 2.67 4.07 14.1 30.3 74.7 172 493 Analyses were conducted on the omposte 1100 mL test solution belore I, was 5 S J s , f w e T < f f i ^ fmm oflnh test concentration. replic (t0,al tour rep"ca' ' Co,"Pa, ny sanitiz e</. D,oes not cm, tjs, C4 CB/ 10 DuPont HLR 591-92 STATIC, ACUTE, -H O U R _EC 50 OF TABLE 3 Water Chemistry of the Control at T e s t s Total Alkalinity (mg/L as CSCO3) EDTA Hardness (mg/L as CaC03) Conductivity (ixmhos/cm) iS Cqi y s an Joas nas _ 11 DuPont HLR 591-92 STATIC. ACUTE. 48-HOUR EC50 OF TABLE 4 Measured Concentrations (mg/L) H-1901 Start pjficnivnri o?YQpn Control (0.45) 2.67 4.07 14.1 30.3 74.7 172 493 8.6b 8.7b 8.6b 8.6b 8.5b 8.6b 8.6b 8.6b Control (0.45) 2.67 4.07 14.1 30.3 74.7 172 493 7.4b 7.4b 7.4b 7.4b 7.4b 7.4b 7.4b 7.4b A. B. C. and D 48 Hours 7.1 7.5 7.2 7.4 7.3 7.1 7.3 7.5 7.3 7.3 7.2 7.4 7.2 7.4 7.2 7.4 7.4 7.3 7.3 7.4 7.3 7.4 7.2 7.4 7.4 7.4 7.3 7.4 7.3 7.3 72 7.3 73 7.3 7.2 7.3. 7.2 7.3 7.3 7.3 7.3 7.3 7.2 7.3 7.3 7.3 7.4 7.3 7.3 73 7.3 7.3 7.2 7.3 7.3 7.3 7.3 7.3 7.2 7.3 7.3 7.3 7.3 7.3 five daphnids each at test before it was divided into COt11Pany San'iftie: ed. e s 'Jof 12 eodfa/n TScA CBt III DuPont HLR 591-92 STATIC ACUTE. 48-HOUR EC50 OF - STAH-19518 TO Daphnia magna TABLE 4, Continued i anH Chemical Characteristics of p h y s ic a l solutiors H-1951 Tip?rfitiire- itrol (0 45) 2.67 2 0 .3b 20.3b -c - - 4.07 14.1 30.3 74.7 172 2 0 .4b 20.3b 2 0 .3b 20-3b 20-4b 20.3b ' - - " * 20.6 20.7 20.7 20.6 20.7 20.7 20.6 20.7 20.6 20.7 20.7 20.7 20.7 20.7 20.7 20.7 20.7 20.6 20.7 20.7 20.6 20.7 20.7 20.7 20.7 20.7 20.7 20.7 20.7 20.7 20.6 20.7 C. and D represent * f each s0| each- test i divided into COn>*J3asinty S i*nfg'{j Oi'estint cftia, tsCa CQf 13 DuPont HLR 591 -92 c-rATir ACUTE 48-HOUR EC50 OF STAH-?9518 TO Daphnia magna TABLE 5 Observed Test Observed Immobility in Percent Measured incentrations (mg/L) daphnids eacn a, test C0i 'ys,toon, '<>estoof,,. ^ in Ts Ca 14 DuPont HLR 591 -92 STAS 3 ^ 0 D l p S a " a OF TABLE 6 n. causing immobility to 50% mcentrations Ca intervals n -195' * magna at spe - Time 24 Hours 48 Hours EC50 (mg/L)a NAb 71.7 95% Fiducial Limits (mg/L)a tower Upper NA 52.9 NA 98.3 ^ upon no, * < * " > - a u s e o, 'S S S X S & ' - Cqi'Pa,y s ,ft!; * * ftoes not Co;`fain TsCz <*/ 15