Document YDYGD8QQK4mr81wpoxw5L89vV

M &Z6 - 0 7 2 9 ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE_________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206 or L3243. (1-Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was 501. The test sample is FC206. Current information indicates it is a mixture of 0.67% PFOS, 17.5% diethylene glycol butyl ether, 78.91% water, 1.33% Sultone foamer, 1% sodium octyl sulfate, 0.04% sodium lauryl sulfate, 0.5% polyoxyethylene monooctylphenyl ether, and 0.05% benzotriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD:____________________________________________________ Method: Not given. Type: Acute static GLP: No Year completed: 1976 Species: Pimephales promelas Supplier: Not given. Analytical monitoring: Dissolved oxygen, pH, and temperature. Exposure period: 96-hours Statistical methods: LC5ovalue calculated using graphic interpolation. Test fish age: Juveniles. Length and weight: Average length = 50 mm Average weight = 1.5 g (wet) Loading: Not given. Pretreatment: None Test conditions: Dilution water: Carbon-filtered well water. Dilution water chemistry: Not given. Lighting: Not given. Stock and test solution preparation: Not given. Concentrations dosing rate: Once. Stability of the test chemical solutions: Not given. Exposure vessels: Not given. Number of replicates: 1 Number of fish per replicate: Not given. Number of concentrations: five plus a blank control Water chemistry during the study: Temperature range (0-96 hours): 21 - 22 C 001523 pH range (0-96 hours): 7.0 - 7.0 mg/L (control exposure) 6.7 - 6.9 mg/L (1250 mg/L exposure)* Dissolved oxygen range (0-96 hours): 5.0 - 5.5 mg/L (control exposure) 4.0 - 5.2 mg/L (1250 mg/L exposure)* * 1250 mg/L (intermediate concentration) exposure values used because the highest three concentrations resulted in total mortality by 72 hours. RESULTS___________________________________________________ Nominal concentrations: Blank control, 500,1250, 2500, 3750, and 5000 mg/L. Element values: 96-hour LC50 = 1500 mg/L Element values based on nominal concentrations Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ The FC-206 96-hour LC5o for fathead minnow was determined to be 1500 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_______________________________________________ Reliability: Klimisch ranking 3. Testing lacks record of methodology used and documentation. The sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES_______________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, 1976. OTHER_____________________________________________________ Last changed: 6/28/00. 001524 TOXICITY work sheet t y v T e ,t-------96 Hours - Material TestW J a g ^ ^ ^ ^ > f g l t Test Organism Fathead Minnow_______ Avg. Weight _ L <TG S iz e 5 > W Date S ta r t )_____________ G / Z C / l A Date Completed l<i( Time Started Z :0 0 f/Vi, Plotted LCSO- Mhi2(? tttf/K D ilu tio n Water Carbon F ilte r e d - Well Water 24 hrs t D.O Control 400 7 ,0 L u l r \mr> Z o togo, . . . . t o 2 . 5 .2 S'.oo loo 6 * sj a, 7b 4? S-noo . AS. Average Temperature 2 2 48 hrs % D.O -KiL.JtiZ L . loo 5 2 72 hrs % u ivx v a i J2 L lo o 7 .0 D.O ppm 96 hrs % D.O survival J f f i . Ido rn 7 .0 lo o 7,o $ . 0 0 0 V 7 .0 4.1 loo 7 * 6 .? p 7<2 6,7 45 So 6 1 45 a 6,7 4 .0 0 6 7 4 .2 . 0 0 0-- In . ..- , ........ 2 J l 6 e 7 <?.6 O o o - 2 1 _ 1_______ It BEST COPY AVAILABLE % Survival