Document YDY4BMwJv5Q8NwNa3BooBGOBO

BIODEGRADATION (MANOMETRIC RESPIROMETRY -AEROBIC) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component ofFC-3041. (1Octanesulfonicacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was notnoted. The testsample is FC-3041. Currentinformationindicatesitisa mixtureof6% PFOS, 36% diethyleneglycolbutylether,36.05% water,12% Sultonefbarner,9% sodium octylsulfate0,.55% sodium laurylsulfatea,nd 0.4% tolyltriazole. The followingsummary appliesto a mixture with incompletely charactetizedconcentrations of impudties. Date may not accuratelyreflect the biodegradabilityof the fluorachemical component of the testsample. METHOD Method: Manometric RespirorrietTreyst Test type: Ready biodegradability GLP: No Year Completed: 1993 Analyticalmonitoring: Oxygen consumptiondeterminedby measurement of the amount ofoxygen generatedelectrolyticatlolmyaintaina constantgas volume inthe respiratiofnlask.Depletionofdissolvedoxygen was registered automaticallyA.mount ofoxygen taken up isgiven (aftecromparison withthe blankinoculum)as a percentage ofthe chemicaloxygen demand. Statisticamlethods: Resultswere determined by calculatioonfthe percentage biodegradation. lnoculum source: Activatedsludge collectedfrom a community sewage plant (Schmallenberg,Germany) thatwas fed almostexclusivelwyithresidential wastewater. Test condition: Dilutionwater: Not noted. Mineral nutrientmedium: MineralBasal Medium Stock and testsolutionpreparation: Solutionsofthe testand reference materialswere prepared at 100 mg/L withmineralbasalmedium. Test vessels: Closed flaskskeptata constanttemperature. Each vessel isconnected gasbght tothe pressureregulatorcorrespondingto it. Incubation Conditions: Per manometric method Temperature: 22 2 OC Agitation:Continuous stirring Number of concentrations: Test sample, testsample inhibitedt,est sample plusanilinea,niline(referencesubstance)and blankinoculum,all induplicate. lnoculumconditioonn testinitiatioInn:itisaollid3sg/L,30 mg solid mafter/L. Element Basis: Biologicaldegradation by means of oxygen consumption, mg 02/L. Test substance flask conditions: pH: Not noted. D.O.: Automaticallmyeasured. RESULTS Nominal concentrations: Blank controlt,estmaterialat100 mg/L,and aniline (referencesubstance)at 100 nig/L. Element values: 14-day DegradationwithTest Material= 93.8% based on average oftwo replicatemsinus the blank. 14-day DegradationwithTest MaterialplusAniline= 88% based on average oftwo replicatemsinus theblank. 14-day DegradationwithTest MaterialInhibite=d 8.5% based on average oftwo replicatemsinus the blank. Remarks: Tesfingwas conducted on a mixtureas describedinthe Test Substance Remarks field.The values reportedapplytothatmixtureand notthe fluorochemicalproporfionalone. CONCLUSIONS The testmaterial% degradabon based on the mean oftwo replicatemsinus the oxygen consumption ofthe blankcontrolwas 93.8% after14 days. The referencesubstance obtained82% degradabon by 14 days,thusthe validaboncriterioonfthe testwas fulfill(e>d60% after14 days). The testwas terminatedon day 14 sincethe validitcyriterioonfthe testwas fulfillaendd the oxygen uptake reached a plateau. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul, Minnesota,55133 DATA QUALITY ReliabilitKyJ:imischranking3. Lacks specifimcethodology used and documentation. Sample puritywas notproper@ycharacterizedand the study lacksanalyticaclonfirmationofthe amount offluorochemicalproporbon inthe soluton. REFERENCES This studywas conducted by FraunhoferlnsbtuteforEnvironmentalChemistry and Ecotoxicology,D-57377 Schmallenberg,Germany atthe requestofthe FederalBureau forMaterialTechnology and AcquisitionsG,ermany, Grant No.: E/B31 E/PO078/N52275, 1993. OTHER Last changed: 6/26/00 FINAL REPORT Eco-ToxicoLoGicAL EFFECT OF FLUOR06SURFACTANTS Grant No.: EIB31E/PO078/NS27S Client Bun&samt ftiWraiaechm-k und Bachaff-ung,Kobleiii (FederalBureau forMaterialTechnology and Acquisition@ InvestigatinLg2sdtute: FraunhoferInstitute forEnvironmentalChemistryand Eco-toxicology D-57377 Schmallenberg InstitutMeanager. ProL Dr.W. Kldn Projectleader. Dr. P..Debus Projectauthom Dr. A. FUedner Dr.K. Hund DipL BioL R. N-mmann Dr. A. Remde Dec=ber 1993 Tandator Andma-InSM Sdkwider.715-549-573(4G=m #1453/1995) Fmunhofer Institute forEzivironmenudChemisuy and Eco-toxicology D-57377 SchmaUenberg Investiptiofnor theEcd-toxicologicEavlaluatioonf theFluoro-surfactaBnatse PC-3041. AerobicDeM&don Partf by Dr.A. Remde Tmulaw. An&ea-InSM Sdmdft. 715-549-5734(G=m 2 #1453/1995) AEROBIC DEGRADATION lU determinatioonf theeasybiologicadlegradabffitoyf Base FC-3041 underaerobic conditionswas done inthemanometric-respirometrtyest(8)'. MATERLAL AND METHODS Testsubstance: Base FC-3041 (combinationoffluoro-surfactainntasqueoussolution; Manufacuuw. 3M Company Germany, Neuss). Testcriterion: Measurement ofthebiologicadlegadabffitbyy me= oftheoxygencoiwimptioil Test metted: A definedvolume of an inoculatemdmeml medium witha known concenuudon of thetestsubstance(100mg/L) issdrredina closedflaskata constanttemperature (22 'C 2).The oxygen consumptionisdeterminedthroughthemeasurement ofthe amount of oxygen generatedelectrolyticatlhlaytisnecessarytomaintaina constant gas volume in therespiratiofnlask.The generatedcarbondioxideisabsorbedintoa solutionof potassiumhydroxide.The amount of oxygen takenup by the test substanceisgiven (aftecronsideringthecorrespondingvalueinthe inoculum blank testperformedinparaIU) aspercentageof theCSB I(chemicaloxygen demand). Testprocedure: 10 testvesselswere preparedfortheperformanceofthetest VesselI and 2: mineralbasalmedium + inoculum Vessel3 and 4:' mftwimlbasalmedium + referencseubstance(100mg/L) + inoculum(30mg solidmatter/L) inEapsh:COD.Iwillpmsem ft'Gemm abbmviadonds= theyam presenitnfoanubsandtables. TrandatorAndma-IngM Schikeida7,15-549-573(4G=m #145311995) 3 Vessel5 and 6: mineralbasalmedium + ma subs== (100mg/L) + inoculum(30mg solidmatterAL) Vessel 7.and 8: mineralbasalmedium + referencesubstance(100mg/L) + testsubstance(100mgll)+ inoculum(30mg solidmatterAL) Vessel9 and 10: mineralbasalmedium + = substance(100mg/L) + inoculum (30 mg solidmatterAL)+ mercury chloride solution(0.001% finalconcenwadon) ne vesselswere closedand placedintothe respirometerA.ftertemperatureand pressurecompensation(approx.0.5 hours),each vesselwas connectedgastightodw pmu= regulatobrelongingtoiLDuring thetestperiodthedepletionof dissolved oxygen was registereadutomatically. The bio-degmdationwas determinedaccordingtothefollowingequation: Percentagebio-&Smdation = (BSB -B) _ x 100 CSB orTH.SB BSB B CSB IISB = biochemicaloxygen consumptionofthetestsubstance(mg) = depletionofdissolvedoxygen ofthebasalmedium withinoculum(mg) = chemicaloxygen consumption2(Mg) = theoreticaolxygen demand (mg) Inoculum: Activesludgefrom a community sewage treatmentplant(SchmaUenberg)which was fed almostexclusivelwyithresidentiawlastewater,was used asinoculum.Ilieactive sludgewas aeratedduringtheb-ansporllUe inoculum was adjustedwithisotonic Ringees soiutioanstoolidscontentof 3 gIL and aemted.Ile samplecollection occurredon 10/2811993. 21nft tM above.CSB wu chemiskrSauerjtoj*ai,mwfhichftandontochmwd otygendemaxd In thiasm ft sme abbmviadoiCmSB is.usefdordwmischerSaueritoffverbnwuhwikchUzWato to chemicaolxygencoxsumpdox. Tmmdatw. Andrea-InVWSdneider7,15-549-573(4G=m #1453/1995) 4 Analysis: To determinethe dissolvedoxygen depletiona, sapromate of the Voith Company i*m)was used.TU oxygen consumptionwas automaticallyregistereads BSB/L Referencesubstance:siniii(Merck,reagengtmde) Testperiod:10/29/1993- 11/12tl993 RESULTS ne resultasrerepresenteidnTab.fl and Fig.fl. During theincubationperiodof 14 days,82% ofthereferencesubstanceanilinwas degraded.Mm validitcyriterioaf thetest(degradatioonf aniline>40% after7 days and >60% after14 days)were fulfiued. 93.8% of the testsubstanceBase FC-3041 was degradedin thesame periodOf time. Sincealready88.6% of the substancehad degradedwithin10 days countedfrom the day the degradationfor the firsttime surpassed10%, the substancemay be categorizedaseasilybiologicalldyegradablrIle testcouldbe temiinitedon day 14 sinceallvaliditcyriterioafthetesthad been fu=ed and a plateauwas reachedfortheoxygen uptake. EVALUATION Based on the presentresultst,he testsubstanceBase FC-3041 was categorizedas easilybiologicalldyegradable. TransWw. AAdma-InSM Sdkidft, 715-549-573(4German#1453/1995) 5 Table fl:BSB and % of biologicadlegradationof the testsubstanceFC-3041 and of the reference SUbSta=,2nilin Starotfthe= Testsubstance(TS): Testconcentration: Chemicaloxygen consumption(CSB): Referencesubstance(RS): Testconcentmtion: Theoreticaolxygen consumptionabSB; NH3) inocuium 10/29/1993 Base FC-3041 100 mg/L 0.88 mgtmg substance Purity:anili 100 mg/L 2.41mgtmg substance activesludge Day3 LNXWM Refacm substance Testsubstance RS + 73 13 + HgCI2 z RSB 1) X USS 21 z ssa 3) t BSB 31 E MB 4) ag 02/1 el 02/1 %degradationmg C)2/1 X degradatioang 02/1 Xdegradation ag 02/1 1 9,5 2 l4lD 3 18,0 4 20,5 5 '&1.2,5 6 24,5 7 26,0 8 2810 9 2915 la 30,5 !1 42,0 12 3215 13 34,5 14 34,5 010 coo 110 1415 5710 114,5 165,3 175,5 184,0 18805 i91's 194.5 19510 197,5 010 oto 0,4 goo 2317 4795 68,7 72,8 76,3 76,2 79,S 90,7 81,3 8zlo 10,5 1790 22,5 34,0 53lO 66,5 71,0 74,0 76,5 7660 -9,5 gi.o 82,5 82,5 1119 19,3 25,6 3816 60,2 75,6 8097 84,1 8699 Ga's 90.3 92,0 93,8 93,8 10.0 14,5 33,0 17290 23810 256,5 26665 272,0 276,5 281*0 284.0 287,0 28915 289,5 3.0 2,0 4,4 3,0 1090 3,0 52l3 490 72t3 5,0 78,0 5,0 al,o 510 82,7 6,0 84,0 6,0 85,4 7,0 86.3 7,0 9712 7,0 8810 sla Salo 815 1)Inoculumcontrol 2)Average valuefrom two experimentalruns,minus theoxygen consumptionby theinoculum control 3)Average valuefrom two experimentarlun-,minus-theoxygen consumptionby theinoculum control 4)Testsubstancewithinoculumand HgCI2 solutiona,veragevaluefrom two expenmental runs 310theoriginaolnlyg b visg)lBcy analogytomWe Sl on pageg/6,ft headingofthiscoll-nmug be Tag whichbumslateassday. TmasLvw. Andmik-IiigWSdmeider,715-549-5734(G=m #1453/1995) 6 Fig.fl: Biologicadlegradationof the testsubstanceBASE FC-3041 and of the referencseubstanceanilininthemanometric-mspimmeuy Z Degradation 100- so ou 40 4mo /0 4y 0 0 1 2 3 4 5 6 7 8 9 -10 11 12 13 14 DAY Bass FC-3041 (TS) TS RS Aniline (RS) MwWatw. Andma-InVU Sdmd4w, 715-549-5734(G=san #1453/1995) 7