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. AR 226 --- 0913 MICROBICS MICROTOX TOXICITY TEST TEST SUBSTANCE Identity: ArefmeirxrteudretocaosntPaiFnOiSng,pFeCr-f8iu5o,rooorcatsanaescuolfmopnoantee,ntwhoifcFhX-m3a10y3a.lso(1b-e Octanesulfonic acid) (CAS # 2795-30-3). R2ise0Fm%Xa-rd3ik1es0t:3h,yl3e%nT.eheglCy3ucrMorlepbnrutotdyiulncfetotirhomenart,lio5otn0n.ui5nmd6bi%ecrawtwaetasesri,tins1oa5t %mnoietxtethduy.rleTeohnfee0gtl.eys9cto6ls%,aP4mF.pOl3Se2,% pSroodpiaunmeocstuylltosnulefaftoea,me6r.,0%0.p5o%lysooxydeituhmylnietnreatme,on0o.o0c6t%ylspohdeinuyml eltahueryrl, s2u.lf5a%te, and 0.1% benzotriazole. B cThhaerafcotlleorwiiznegd scounmcmeantrryataipoplnisoesftiompaumriitxiteusr.e Dwaittha imnacyomnpoltetaeclcyurately roflect the toxicity of the fluorochemical component of the test sample. METHOD: Method: Microbics Microtox "BASIC" Procedure Test type: Static acute GLP: No SYpeeacriCeosm:plPehtoteodb:ac1t9e8r5ium phosphoreum AEnxaploystiucrael pmeorniiotdo:ri3n0g-:miNnouttegsiven. Statistical methods: EC50 values calculated by statistical linear regression TeprsotgroarmgparnoivsimdesdofuorrcMei:crMoitcorxobics' Reagent. Test coDnidliuteinot:ns:Not given. TSetsotckteamnpdertaetsutrseo:luNtoitonnoptreedp.aration: Not given. Exposure vessels: 4 mL cuvettes. NNuummbbeerr ooff creopnlciecnattreast:iotnwso: Four plus a blank control Element basis: Percent light loss. RESULTS --_-- Nominal concentrations: Blank control, 100, 250, 750, and 1000 mg/L. Elementvalue: 5-minute 15-minute ECso ECso = = >>11000000 mmgg//LL 002974 Element values based on nominal concentrations. Remarks: Substance TReesmtairnkgswafiseldc.onTdhuectveadluoens a mixture reported as described inthe Test applyto that mixture and not the fluorochemical proportion alone. CONCLUSIONS The test substance 15-minute Eso for Photobacterium phosphoreum was determined to be >1000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133 DATA QUALITY pon -- Rmeeltihaboidliftoyl:loKwieidm.isTchherraenkisinngo3.chaSrtauctdeyrilzaactkisodnoesfctrhipetitoenstasnudbsrteacnorcdesopufritthye, and the study lacks analytical confirmationofthe amount of fluorochemical proportion in the solution. REFERENCES TMhiinsnesstoutday,wLaasbcRoenqduuecsttednubmybtehre C31M11E1n-v1i,ro1n9m8e5n.tal Laboratory, St. Paul, OTHER EE Last changed: 6/26/00. 00297 oC EARONMENTAL LABGRATORY | TTT MiMcicrroos, TRoAxiWc.itDyATTeAst DATES Feb 192.47 Austin Save Desc EX:3r 10si 3%Tp ype n bighti woo ke n: [Savin (Wy BL Soca B2 cz Bs C3 aN cH B5 cs Finan Assa( Concensmanod Bran DuricATE Reading] Fingi S5-Min| ReApinGs 15-MIN|SO- Mn 0) | I(s) | T(s)| T(30) 90 55 32 20 qo | sf | 3%| 2a 100 wg 2 deplicode Moles | de | 38 83 | 52 [33 | 30 as0wgle Gupli cade 58 | 6 | 30 | ay 9 4. | az 2 150wyle duplicate 9% | 38 | 23 | 138 18 30 F 1x 1000 ile duplicate 3g | 28 | re 34 29 | 16 BEST COPY AVAILABLE 00297 Lo. MicroroX Dara REucion Bang Famas* = Sh |= ITE DATES fog pr Avast Lies C81 a % C__ct Thee *The Bran "RAMOS Fow, BL Aud CL SHOUD AGREE. WITHIN. 0:02. Mean Bra Ranna = Bd RaaRatio 2 RICRakia oo PE NORMALIZED LAGHT ho ce ERECT [o1 s 2s e ] Mean RPa atioxk tial Sawgle Feading = FinalDompic Keading -- gLcoesn ris oh Tew L576 B5 Io Lesy JHU "0.0 Lous Er "633 43h LBe] ic.338 "0. "243 eB2r In % iH RIO: Yo A) = IST X00 le | 8g | eestoordamume srrr es receive CoOncenTRATION [sce ['s|-asm -mim a | soo E Cor, (A576Conic Goi] 1324, (12012 =ao tes (avs=1253.1) Ely, > toon Ey, tooo 00297"