Document Y6y2NMyKQM174w7dnogXEMbV

/1IUX-0M7 ACUTE TOXICITY TO AQUATIC PLANTS (ALGAE) TEST SUBSTANCE Identity: Perfluorooctanesulfonylamido(ethyl)acetate; may also be referred to as PFOSAA, FC-128 or as the major component of FC-127, FC-129, FC-109, FC-109-X or R1904. (Glycine, Nethyl-N-[(heptadecafluorooctyl)sulfonyl]-, potassium salt, CAS # 2991-51-7) Remarks: The test sample is FC-129 referred to by the test laboratory as R1904. Current information indicates it is a mixture of 42% test substance, 32% water, 14% 2-butoxyethanol, 4% ethanol, and 8% C4-C7 fluoroalkyl carboxylate compounds. Test material was from 3M production lot 930014, mfg. date 3/97. The following summary is abbreviated because the test sample is a mixture of the test substance in a water/organic solvent solution. Data may not accurately relate toxicity of the test sample with that of the test substance. METHOD: Methods: OECD 201 Test type: Static acute GLP: No Year Completed: 1997 Species: Selenastrum capricornutum Analytical monitoring: Temperature, pH, and conductivity RESULTS Nominal concentrations: Bk control, 129.6, 216, 360, 600, 1000 mg/L (4 replicates per concentrations, 6 per blank controls) Element values: Endpoint Cell Count Data (based on nominal concentrations) Time 24-hour E C S0 48-hour EC50 72-hour E C 10 72-hour EC50 96-hour E C 10 96-hour EC50 96-hour NOEC (mg/L) 431 395 113 248 163 298 216 95% Confidence Interval (mg/L) 385-484 356-439 94-130 225-272 54-228 278-319 Not Applicable 000400 Endpoint Average Specific Growth Rate (U) Data (based on nominal concentrations) Time 24-hour EC50 48-hour EC50 72-hour E C 10 72-hour EC 50 96-hour E C 10 96-hour EC50 96-hour NOEC (mg/L) 586 859 234 610 287 604 216 95% Confidence Interval (mg/L) 524-656 777-950 197-268 551-676 248-322 550-664 Not Applicable Remarks: Testing was conducted on the mixture of the test substance as described in the test substance remarks field. The values reported apply to that mixture and not the test substance. No attempt was made to determine the impact of the presence of the organic solvents or what portion of the toxicity can be contributed to the Perfluorooctanesulfonylamido(ethyl)acetate. CONCLUSIONS The FC-129 96-hour EC5ofor Selenastrum capricornutum was determined to be 298 mg/L (C.I.: 278-319 mg/L) by Cell Count and 604 mg/L (C.I.: 550-664 mg/L) by Average Specific Growth Rate. The 96-hour no observed effect concentration for both Cell Count and Average Specific Growth Rate was 216 mg/L. Effect determined to be algistatic based on recovery of cells previously exposed to the 1000 mg/L concentration. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 2. Testing meets all criteria for quality testing, but lacks analytical confirmation of test substance concentrations. There is a lack of characterization of the test sample. REFERENCES This study was conducted at AScI Corporation of Duluth, Minnesota at the request of the 3M Company. OTHER Last changed: 5/17/00 0041 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 STUDY TITLE GROWTH INHIBITION OF R1904 FOR GREEN ALGA (Selenastnm capricomutum) DATA STANDARD OECD GUIDELINE 201 STUDY COMPLETED June 18, 1997 TESTING FACILITY AScI Corporation/AScI-Duluth Environmental Testing Division 4444 Airpark Boulevard Duluth, MN 55811 Tel. No. (218) 722-4040 Fax No. (218) 722-2592 STUDY IDENTIFICATION NUMBERS AScI Study ID# 5010-068 3M Company Lab Request# R1904 Sponsor: 3M Company Sponsor Study ID# R1904 1 000402 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 1.0 GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor's Representative Sponsor's Lab Request# Testing Facility Principal Investigator Project Director Testing Facility Director Definitive Test Dates Data Validation and Report Review Growth Inhibition of R1904 for Green Alga (Selenastrwn capricomutum). OECD Guideline 201. 3M Environmental Technology and Safety Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133; Tel No. (612) 778-7452. Susan Beach, 3M Environmental Technology and Safety Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133; Tel No. (612) 778-7452. R1904. AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. Todd McGuire Joe Amato Donald Mount June 9-13, 1997. Alan Mozol Report Signature: Retention of Raw Data and Final Report Location of Raw Data and Final Report Two years from the date study is completed. AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. Sponsor: 3M Company Sponsor Study ID# R1904 2 000403 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 2.0 OBJECTIVE(S) To determine the 3-d and 4-d effect concentrations (EC10's and EC50's) and the 4-d no observable effect concentration (NOEC) of the test substance, R1904, for green alga (Selenastrum capricomutum) under static test conditions. This study was conducted according to OECD Guideline 201 (OECD 1993). 3.0 TEST METHODS Test Substance Test Organisms Algal Medium Exposure Chambers Properties: R1904, amber liquid, specific gravity ca. 1.3 (water = 1), stable, and completely soluble in water. Test concentrations: 0 (algal medium control), 129.6, 216, 360, 600, and 1000 mg/L. A 2000 mg/L stock solution was prepared by mass addition to algal medium followed by mechanical stirring. Each test concentration was made by volume addition of the stock to algal medium. Source: ATCC 22662 Inoculum preparation: Algal stock was subcultured in OECD recommended medium for 4 days before test initiation. Use in test: Stock culture was diluted and spiked into test solutions to obtain an initial cell density of 1.0 X 104 cells per ml (1.0 X 104 actual concentration). Preparation: Deionized water was spiked with nutrient stocks (including Na2EDTA) to OECD recommended concentrations. The most recent chemical analysis for deionized water is in Raw Data Package. Characteristics: At test time, the medium had a pH of 7.86. Sterilized via 0.22 fim filtration prior to use in the test. Type: Sterilized via autoclave 250-ml borosilicate-glass Erlenmeyer flasks sealed with foam stoppers. Use in test: 100 ml of algal medium or test solution/chamber. Six control replicates and four replicates per test substance concentration were employed. During the test, kept stoppered, except when experimental observations were made. Sponsor: 3M Company Sponsor Study ID# R1904 3 000404 Incubation Observations Endpoint Calculations AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 Duration: 4 days. Daily photoperiod: Continuous at 740 ft-candles using cool-white fluorescent lamps. Test chambers held on shaker table set at 100 rpm. Temperature: 25.0 2C. Biological: Daily cell counts using direct microscopic enumeration via Improved Neubauer hemacytometer. Water chemistry: (1) At test initiation and termination- pH, conductivity, and temperature, (2) daily- temperature. All determinations were made according to APHA methods (1995). 1-, 2-, 3-, and 4-d EC50's, 3- and 4-d EC10's, and 4-day NOEC's based on both cell counts and average specific growth rates (jx). Statistical analyses performed using Trimmed Spearman-Karber Method (ECS0's), Toxstat (NOEC's) and EPA Probit software (EC10's). Sponsor: 3M Company Sponsor Study ID# R1904 4 000405 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 4.0 RESULTS I Test Substance Toxicity (Pertinent data are in Tables 1, 2, and 3) Test Conditions Cell Count 1-d EC5Q: 431 mg/L (385-484). fi 1-d ECsq: 586 mg/L (524-656). Cell Count 2-d ECS0: 395 mg/L (356-439). fi 2-d EC5q: 859 mg/L (777-950). Cell Count 3-d ECl0: 113 mg/L (94-130). H 3-d EC10: 234 mg/L (197-268). Cell Count 3-d ECSQ: 248 mg/L (225-272). fi 3-d ECS0: 610 mg/L (551-676). Cell Count 4-d ECl0: 163 mg/L (54-228). U 4-d ECl0: 287 mg/L (248-322). Cell Count 4-d ECS0: 298 mg/L (278-319). ft 4-d EC50: 604 mg/L (550-664). Cell Count 4-d NOEC: 216 mg/L. H 4-d NOEC: 216 mg/L. Effect was determined to be algistatic based on recovery of cells previously exposed to the 1000 mg/L test substance concentration. Temperature (C): 24.8-26.0. Light intensityft-cdls: 740. pH: 6.78-9.46. Specific conductivity (umhos/cm): 123-189. Sponsor: 3M Company Sponsor Study ID# R1904 5 000406 5.0 TEST QA/QC AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 QA/QC Criteria Algal counts in control must increase by at least a factor of 16 during the first 3 d of the exposure. Test duration must be 4 d. Data During the first 3 d of the test control algal counts increased by a factor of 348. The test duration was 4 d. 6.0 REFERENCES American Public Health Association (APHA). 1995. Standard Methods for the Examination of Water and Wastewater. APHA, Washington, D.C. Organization for Economic Cooperation and Development (OECD). 1993. OECD Guidelines for Testing of Chemicals. OECD Publication Information Center, Washington, D.C. Hamilton, M .A., R.C. Russo, and R.V. Thurston, 1977. Trimmed Spearman-Karber Method for Estimating Median Lethal Concentrations in Toxicity Bioassays. Environ. Sei. Technol. 11(7): 714-719; Correction 12 (4): 417 (1978). U.S. Environmental Protection Agency (EPA). 1988. Computer Program Probit. Biological Methods Branch, EMSL, U.S. EPA, Cincinnati, OH 45268. Sponsor: 3M Company Sponsor Study ID# R1904 6 000407 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 TABLE 1. EC jq's EC5q'S, 95% Confidence Intervals, and 4-d NOEC'S from Algal Growth Inhibition Test with R1904 Endpoint Cell Count Time 1-d EC50 2-d EC50 3-d EC10 3-d EC50 4-d ECl0 4 -d E C jo 4-d NOEC Data* (mg/L) 431 395 113 248 163 298 216 95% Confidence Interval (mg/L) 385-484 356-439 94-130 225-272 54-228 278-319 Not Applicable Endpoint Average Specific Growth Rate (/t) Time 1-d EC50 2-d EC^q 3-d EC10 3-d EC50 4-d EC ,0 4-d EC50 4-d NOEC * Values Based on Nominal Test Concentrations (mg/L) 586 859 234 610 287 604 216 95% Confidence Interval (mg/L) 524-656 777-950 197-268 551-676 248-322 550-664 Not Applicable Sponsor 3M Company Sponsor Study ID# R1904 7 000408 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 50104)68 TABLE 2. Cells per ml and Percentage Difference from Mean Control Value for Selenastrum capricomutum Exposed to R1904 Test Cone. (mg/L) Control 129.6 216 360 600 1000 Replicate A B C D E F Mean A B C D Mean A B C D Mean A B C D Mean A B C D Mean A B C D Mean Id 11 8 8 9 7 10 8.8 (-) 9 6 7 7 7.3 (-17) 10 7 6 8 7.8 (-11) 3 5 6 3 4.3 (-51) 3 3 4 4 3.5 (-60) 2 1 2 1 1.5 (-83) Cells/ml (10*) 2d 3d 52 48 60 51 64 48 53.8 (-) 50 43 50 52 48.8 (-9) 37 45 44 39 41.3 (-23) 24 27 27 28 26.5 (-51) 24 24 17 18 20.8 (-61) 4 6 3 6 4.8 (-91) 342 306 348 336 390 366 348.0 (-) 264 282 318 300 291.0 (-16) 240 204 198 234 219.0 (-37) 72 72 96 84 81.0 (-77) 33 27 30 25 28.8 (-92) 4 2 2 3 2.8 (-99) 4d 605 704 616 572 627 726 641.7 (-) 506 528 627 671 583.0 (-9) 517 561 583 594 563.8 (-12) 143 154 165 165 156.8 (-76) 37 32 32 30 32.8 (-95) 2 3 3 4 3.0 (-100) * Parenthetic Values -- % Difference from Control Mean Value * 0-d Mean Algal Count = 1.0 X 104 Sponsor. 3M Company Sponsor Study ID# R1904 8 000409 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 TABLE 3. Average Specific Growth Rates ip) and Percentage Difference from Mean Control Value for Selenastrum capricomutum Exposed to R1904 Test Cone. (mg/L) Control 1d 2.175 (-) Average Specific Growth Rate (p)* 2d 3d 1.993 (-) 1.951 (-) 4d 1.616 (-) 129.6 1.988 (-9) 1.944 (-2) 1.891 (-3) 1.592 (-1) 216 2.054 (-6) 1.860 (-7) 1.796 (-8) 1.584 (-2) 360 1.495 (-33) 1.639 (-18) 1.465 (-25) 1.264 (-22) 600 1.253 (-42) 1.517 (-24) 1.120 (-43) 0.873 (-46) 1000 0.405 (-81) 0.784 (-61) 0.343 (-82) 0.275 (-83) * It = In Cells per ml t,, - In Cells/ml tq n-to b Parenthetic Values = % Difference fromControl Mean Value * 0-d Mean Algal Count = 1.0 X 104 Sponsor: 3M Company Sponsor Study ID# R1904 00041Q