Document Y0x4EznrawmL0DeK6r2DK0V8
A K M G _ D g J U f-
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
TEST SUBSTANCE_________________________
Identity: A mixture containing perfluorooctanesulfonate; may also be referred to as PFOS, FC-95, or as a component of FC-206CF or FC-206CF-X. (1-Octanesulfonicacid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was 501. The test sample is FC206CF-X. Current information indicates it is a mixture of 0.5% PFOS, 10% diethylene glycol monobutyl ether, 78.08% water, 1.375% acrylic foamer, 1.5% sodium octyl sulfate, 1% sulfonated alkyl ether salts, 6.0% urea, 0.5% triethanolamine, and 0.05% tolyltriazole.
The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample.
METHOD____________________________________________________
Method: OECD Guideline 209 GLP: No Year Completed: 1992 Analytical monitoring: Dissolved oxygen concentrations, pH, and temperature. Statistical methods: Results were determined by calculation of the % inhibition in respiration rate. Test organism source: Activated sludge mixed liquor collected from the Metro Wastewater Treatment Plant, St. Paul, MN. Test condition:
Dilution water: Millipore Milli-QTM water. Synthetic sewage: per OECD method. Reference and test solution preparation: A stock solution of the reference substance, 3,5-dichlorophenol, was prepared by dissolving 500 mg in 10 mL 1N NaOH, diluted to 30 mL with Millipore Milli-QTM water, then brought to the point of incipient precipitation with 1N H2SO4 and diluted to 1 L with Milli-QTM water. The pH of the reference solution was measured to be 7.6. Test substance was added directly to the test vessels. Initial pH 6.8. Test vessels: Not given. Number of concentrations: one (duplicate) plus 1 reference substance and 2 blank controls Temperature: 1 8 .6 -2 1 .6 C Total suspended solids and pH on day of testing: 2.6 g/L (TSS in final: 1.04 g/L), initial pH 6.9 and final pH 7.9. Element Basis: Respiration inhibition as determined by oxygen consumption.
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Remarks: Values were corrected to 20 C for calculations. RESULTS Nominal concentrations: 2 Blank controls, reference substance and 1000 mg/L Elem ent values: 30-minute EC50 = >1000 mg/L
3-hour EC50 = >1000 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS______________________________________________ The test substance 3-hour EC50 for activated sludge respiration inhibition was determined to be >1000 mg/L. The 1000 mg/L treatment caused -18% respiration inhibition at 3 hours. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY______________________________________________ Reliability: Klimisch ranking 2. The study meets all criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES_______________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St Paul, MN, Lab Request number K1905-2, 1992. OTHER___________________ ___________________________ Last changed: 6/28/00
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ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MIXED UQUOR
lr ^ A
Data ir
Analyst S 4 o
Page _J_ of J_
Type Test
4<r4 i \JaAerJ ^ iu A ^ e Q e<^pir<\\-\C*\ X n V > i hif-icry P * 3 c c\
ACTIVATED SLUDGE MIXED LIQUOR
Date Obtained i U t> l < M
Date Used
Mixed Liquor Suspended Solids (MLSS) . < k g/L Adjusted Jo ~
g/L
MLSS in final test solution / & V g/L
Initial pH (> (1st test soin, prepared)
Final pH 7 . f (last soin, prepared)
Comments ___________________________
TEST SUBSTANCE
Sample Description
VC .~ ^ O U C L F - Y
L o t 751
Initial pH of highest cone, tested ( fT-OO
b>-R
Comments ______________________________________________ _
Adjusted to pH
REFERENCE SUBSTANCE Sample Description 3 , S - ~ X > \ c l-\ 1o r o p W in t ? I Initial pH of stock solution [ S 'n O mg/L) - ~7>b Comments __________ ;______________________________
Adjusted to pH
EXPOSURE CONDITIONS
Test Duration _ 3 - f 4 o o r <^
Test Temperature Range: J& . L ~ <Pl, b C
Comments
_____ *" ' - '
.
.V
"j 4&X-. * **?
X'^XXi:';:,-yrX
KX:^-'X;
Sz% -
,`lf -
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b e s t c o p y a v a il a b l e
ENVIRONMENTAL LABORATORY
LR K r? O S -Z l
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 TEST SOLUTIONS PREPARATION
Test Substance: F C - J O L C F ~ X _______________L p- i 5~C> I___________
Sample Description: / 4 r n h < ? r f t p ! A
Analyst:
__________
Dw aa tree:: -J(pp {j * k > j
--
Test Cone. mg added m L M O O ) water m L syn. sewage
m L microbial
m a /L ... Der 5 0 0 m L
oer 500 mL
feed oer 500 mL inoculum Der 500 m L
I n f>Vrcl____________ *______ ________S A U ______ __________ L k _________
^ CTZ>
I O o C l___________ s o o _____________ F S H ______ __________ U p _________
t X p / iVa k>
F Ci O ____________ M A L _______ __________ U z _________
Total Volum e mL &
rj & n
J - >o i 4 - i a J ! ______T > h f -
_________
-,v
0)
MilM-QR high purity water ,
,,v
:"'IkiSvA
V'^
/* ; v ' -v ,:-.:s*:i *;:< g f 1'
01920
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OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
RAW DATA
Test Substance: FT? - i o h C F - X
Source of Activated Sludge Mixed Liquor: A ' j f '-trn (
Date Receiveid:
/ *7^
Analyst:
^ Q (>rxci^\
~Trgcv/>-ngn4- P L r w - .*> tn u f. / a/ Date Used: (o 3. io / 7 2
30-MINUTE EXPOSURE
DISSOLVED OXYGEN CONCENTRATIONS. mgL
Test Substance
TIME- MINUT ES
Temp
Concentration { ^ ) ) 0.5 1.0 1.5 2.0 2 .5 3.0 3 .5 4 .0 4.5 5 .0 5.5 QC
BKCONTROL 1 !r>r>c>
7-g 7 -L 7-4 7 . X 7 . 0 S 0-L> L . H
4 , o *5. p>.5 ^ ).l
3L.H
ia .O > . 7 xfc- J
V t$ T - t 4 ci I 6s>
l^b-pl1/Vi4p 5 . 1
S . I i r r H.H l . l .2
5 .a a .^
aw
BKCONTROLO
7.0 Z . -S '
i - \ 5 .< \ S . l 5 a 2 2 . 5 1 L 1
3-HOUR EXPOSURE
DISSOLVED OXYGEN CONCENTRATIONS, mgrt.
Test Substance Concentration
TIME MINITTES
Temp
) 0.5 1.0 1.5 2.0 2 .5 3.0 3.5 4 .0 4.5 5 .0 5.5
C .
BKCONTROL 1
8 . * 8 . I 7*1 7 h 7 S 7 V 7 .1 7 >
!> p W l g OO > .C r
l i O (<vt
L 7 / . Lf. 1 3 0 . S
'p.'plVKfre
T 1A
1 ,1 (d . I ( 3 (oS <by <r>.a
3 0 .5
BKCONTROLO
' <; SW'<V-
*
"'fjSi.-
- -_
2 3 . 7 .1
6 . 6 .7 M h l V
. '-`tfi
Initial
jV '
/
t*
ra
Initial__ 1 . 7 - -
C to 4 '
*;
0 .0 5 T 2 5-.
S r\ . \
Final
- ' Final 1 4f
V-. 7A. .r" '.'v 4- . ' r
'/.rTVy *!* { .%!>.
'V -fhW
; r *. ' r
5 :4
11b
Q0 1 9 2 1
,
H
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ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 .CALCULATIONOF RESULTS
Test Substance: FC-206CF-X
Analyst: 2AB/RJW
Lr: K1905-2 Date: 6 /2 6 /9 2
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30-Minute Exposure nr?9/ ~ 0.5 1.0
f e i l i ill i; BK1 7.8 7.6
1.5 2.0 "-'
7.4 7.2
Dissolved Oxygen Concentrations. mg/L 2.5 3.0 3.5 4.0 4.5 5.0 5.5
> 7.0 6.8 6.6 6.4 6.2 6.0 5.7
Temp. *C
20.1
R (1) 0.4109
Ra (2) % In h . (3) \S' sy
0.4081
1000.0 4.0 3.8 3.5 3.1 2.8 2.4 2.1 1.8 1.5 1.3 0.9 21.6 0.6400 0.5726
0
1000.0 5.7 5.4 5.1 4.7 4.4 4.1 3.8 3.4 3.2 2.9 2.5
rv BK2 7.0 6.7 -TtT% diff in controls (4):
6.5 1.8
6.3
6.1 5.9 5.7 5.4 5.2 5.1 EC50 (95% Cl): > 1000 mo/L
4.8
21.1 20.7
0.6339 0.5873
0
0.4206 0.4006 RaCm (5): 0.4043
3-Hour Exposure u
mq/L 0.5 1.0 1.5
,
aj
fllM gi
BK1 8.4 8.2 8.1
<- ; *s*.
1000.0 7.6 7.6 7.3 >-stC*.
1000.0 7.7 7.5 7.4
vv
2.0 2.5 I M
7.9 7.6
Dissolved Oxyqen Concentrations, mq/L
3.0 3.5 4.0 4.5 5.0 5.5 Temp, *C
m u m tftl
/ iV/.x'mv'..V
' f*" s? <.'>/ *" ' .
7.5 7.4 7.1 7.0 6.9 6.7
18.6
I
R (1) 0.3394
7.2 7.0 6.9 6.7 6.6 6.3 6.2 6.1 20.8 0.3285
7.2 7.1 6.9 6.7 6.5 6.4 6.2 6.1 20.5 0.3273
Ra (2) % Inh. (3) V Tee. m m m 0.3741
0.3107
18
0.3161
17
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BK2 7.3 7.1 6.9 6.7 6.6 6.4 6.2 6.0 5.8 5.6 5.4
% diff in controls (4): 2.9
EC 50 (95% Cl): > 1000 mo/L
19.6 0.3745 0.3851 RaCm (5): 0.3796
O O' *: 5 0 0
(1) Ra Oxygen uptake rata (mg 02/t-mln) ' (2) Ra. R adjuilad to 20*C. [fVa.Ex) ^ - ; >t (3) % InNbMon. I(RaCm)-RaS/(RaCm)Jt00 iijr.-.- (4).% dMaranoa In (ontreta: pRaBk1-RaBk2VRaCm)100 (Two control* must be within 15% o< each other.) 'v C - (5) Mean articled B, control*. [R*6k1RaBk2]a
e . 1.072 RaCm. mean adjusted R. control* Bk blank control, S sample Tobs sample temp > Tobs-20'C
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OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #2 0 9 TEST SOLUTIONS PREPARATION
Test Substance:
/ire-* p>h<?hs\t_____Q>en?r\c*p o h & k in ce
Sample Description: A - \ d f I f J u ___ f c k J . !< *M A ____ I c r t > 7 , O & O -O ________
Analyst:
_________
Date: / t e / 4 Z ___
`
Stock Solution
' t S s<r \ y <>ct S o o
^ 7 T t a
iiufgct +g> y <r*QS u.i4-U, Ollh-CC
a itylQ - O S-=>mrnn a c / j / T J V<*C<fj]
iLW ler_______
4 Ulf ci 7TO AS
r l rc-pu,-is.e u'lrtfil m c t p i e n + p > [ H -
r > i k 4 e c t -t-c o n e liier__u , i 4 U . __Al III-G . i>tx4pr~
S f^ rk Svolto
~ ^-b
mL M t1) water per 500 mL
JiS JL
mL syn. sewage
fcgti P9f. M O m L
mL microbial inoculum pe r 500 mL
JZ&D
Total Volume mL
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OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
RAW DATA
Test Substance: 3! J?-
)orop>]npno\
.S 0
Ce________
Source of Activated Sludge Mixed Liquor: J A p -lm IXJnS^eL I f p g 'f/n e n f-' 'F jg n h
Date Received:
7
Date Used:
J
Analyst:
0^ C (X fh
-
M aJ
30-MINUTE EXPOSURE
Test Substance Concentration
) 0.5
1.0
DISSOLVED OXYGEN CONCENTRATIONS, mgl.
TIME-MINUTES 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0
Temp 5.5 C
BKCONTROL 1
m
7$ 7\c 7 4 7. . 7 .e .8 > L M
. o (5*. 7 ^ 1
1.5
1 , 3 1 .2 L "1-0 E T
6 .6 6*5
SO.*3
BKCONTROL B
M ) 6 , 1 6.5 fe 3 (>,1
S I 5 1 5 .3 5.1
3-HOUR EXPOSURE
;* v \ v '
DISSOLVED OXYGEN COfCSYTRATJONS, mv^1L*v.
aai
&
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iiggENVIRONMENTALUBOFWORY : OECDACTIVATEDSLUDGE RESPIRATION INHIBITION TEST #209 CALCUiATIONOF RESULTS y * t *. *
H ^ ffi^ T e s tiS u b s ta n o e :'. 3.5-DICHLOROPHENOL
: Analyst:'SAB/RJW
DaLtre:: K61/920S6/92
% 3^3
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|ma/L*l *0 .5 *1 1.0 I 1.5 \ 2.0 I 2.5 3.0 3.5 4.0 4.5 5.0 5.5 Temp. *C R (1), Ra (2) % Inh. (3)
>[.'...
aa&a&M&fe ILijUt.'i<.u*iAi
S t
' 'r& fy ; ' >* ><';i >y ;,v-
m i
BK1 k ;7;8 * 7 .6 7.4 7.2 7.0 6.8 6.6 6.4 6.2 6.0 5.7 20.1 0.4109 0.4081
i,.'i
10 >7.8* i*7.5 7.5 >7.3> * 7.2 7.0 6.9 6.8 6.6 6.5 6.3
*1yi\".V '"I
tWfiv.
>.- .
20.8' 0.2739 0.2591
36
SwSBiPW*'
MRfe j^A^xv-^v v-r
^ V :
rjA ,,.,.- 'v - i
BK2i* ?7i0* *6 ;7 6.5 S 6.3 >6.1 -5 .9 5.7 5.4 5.2 5.1 4.8
% W i n controls (4): :v> 1.8*";; / -
.. EC50 (95% Cl):
20.7
0.4206 0.4006 RaCm (5): 0.4043
3-Hour Exposure
mg/L_ * 0 . 5 ' 1.0 I 1.5
hb
HnH F
BK1- >>8.4 -38.2" 8.1
t-r
X 10 8.1* 8.0 8.0
- Dissolved Oxyoen Concentrations, ma/L
I 2.0 2.5 3.0 3.5
IP fiW
aiuMmy *
mmm
i
M
ii
7.9 7.6 7.5 7.4
4.0 7.1
4.5 5.0
'>" - - i* I U V A
7.0 6.9
5.5 6.7
7.9 7.8 7.7 7.6 7.6 7.5 7.4 7.3
I T#mp. *C 18.8 20.7
R 0 )^ R a ^ |% Inh. (3)
0.3394 0.3741
0.1600 0.1524 60
V.'lXI-.-i' afc& i#
<BK2 >7.3 7.1 6.9 I 6.7 I 6.6 6.4 6.2 6.0 5.8 5.6 5.4
diff in controls (4): * s 2.9 '
EC SO (95% Cl):
19.6
v'*ji
; .- ^ *
-.. -
'
Me(mg 02/L-mln)
m )^^lnN bkm ?|((teC (n)-R aS /(R *C m )]ioo -!- -rs w ^ j r v>
8^(H(S4})pT3%MfoMdM'4nWaldra*ju4ni<ortt**dnoR's1n/cUo8ontie*ronftfpai.Ra;|-RB-akB1-kfl1atBRka28lfkR2eyC2m>]1:0',0:v-(Two oonlrota musl be wHhin 15% ct Mch other.)
e . 1.072 RaCm mean adjusted R, a x ilrota Bk blank control, S sample Toba aampla temp i . Tobe-20*C
0.3745 0.3851 RaCm (5): 0.3796
L--UJ
CD
' SampledBy(
fri
Additional Comments
; V'. ^ ' '
- V 4 - 'i ; : V'
' OROEINAL^v.- .,'"-/_' '- `i
K
|mvee<
r ; V: : :v- ;;
SEE REVERSE SIDE FOR INSTRUCTIONS
iw u iM iw ijia'^i t swip ^ w ffi.iw i j w w w w p i i p i t p j p p p i ^ ^
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