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1474 S.Vandbventbx Avb. . St. Lours 10, Mo.
Norsmber 10, 1953
CERTIFICATE OF ANALYSIS OA
SUBJECT -
Tha Aouts Oral Toxicity (IDB0) of Aroalor 1251| For Rata.
TEST CONDUCTED FCB -
Man*anto Chandoal Company, St, Louia, Missouri. Monsanto Projsct No.r SA-15
KXPLRIMtl.TAL PHOCEDUNr. -
'
Tha U)k0 was datarmlnad by the lntroduotion'of^naaured single dotes of undiluted Aroalor 1Z5U Into ths stomaohs of Sprague Dawley strain albino rats by mans of a rubber oathster attashed to a hypodarmlo syringe. Animals Neighing 175 to 250 grams ue*e ussd In the test.
After ths approximate Minimum lethal Doss was found, groups of animals
uare fad at IstoIs designed to blanket the texiolty range thereby supplying data for oaloulatlon of tha UDgn uhiah was dona aooordlng to a modification of tha method of Rliaa (<juart. J. Pbarei. and Pharmaool., 11, 1938). The data based on results obtained from a total of 1*1 rats are found In Table I.
Seleotad animals from those reoalving a lethal dose Mere autopsied and maoroaoopio examination made of ths visoera.
MCNS 061763
This is a confidential report which may be used when requested by physicians and health officials, but is not to be used in any form of advertising without written permission. CONSULTANTS TO THE FOOD. CHEMICAL AND PHARMACEUTICAL INDUSTRIES
TOi HMMHta Chi' ' 'll Company St. Uni*, ft jowrl
3olanOXla Aaaoolataa Cartifloata of Analgia -- Pa*a 2 (11*10-$])
T1IU I
m LDio OF APOCLOR 1251* FCR RATS
ilnal No.- Sax
V*i(ht Or ana
Doaa Ml./Kg.
1- Mala
2- Mila
3- Mala 1*- Fmala
5- Fainall
6- Mala
7- Fanala
0- Fanala
9- Fanala
10- Mala
11- Mala
12- Mila 13- Fanala
lU- Fanala
IS- Fanala
16- Mala
17- Mala 18- Mala 19- Mala
20- Fanala
21- Fanala 22- Fanala
23- Mala
21*- Mala 25- Mala
26- Mala /
-
27- Fanala .
28- Fanala/
29- Ala/
30- Fanala
31- Fanala
32- Fanala,
33- Mala \
3U- Mala
'
35- Mala
36- Fanala 37- Fanala
38- Fanala
39- Mala U0- Mala
1*1- Mala
22$ 2.50
us
21*0
18$
19$ 220
200
17$ 190
230
250 3.00
215 205 / / \
190 / / X)
\ \ / /180
210 230
21*0 _
22$ \
, 18$ ^ \
19?
- '
N
\\ \
3.2^' \
\
X 230\
1
215
2l*S\ 200
/
180
190
215 209
190 3.75
21*0
230
21*5 180
190
18$
22$
210
21*0
Fata
Surrlrad Dlad
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HONS 061764
TOi snaanto cam .al Coop in/ St* Loaia, Mlaoouri
salaotlflo laaoalataa Cortlflaato of Analyala -- Paga 3 (11-10-53)
DISCUSSIO* -
The LD*a wao oaloulatad to bo 3.10 adllllltora par ldlogran with uppor and lovar lLdto of 2.58 to 3.2li ailUlitara por kilogran. Surriral tlno ranftf ftroa 1 to 6 daya vttta tho majority of daatbo occurring la 2 to 3 day*. Thara vaa modarata diarrhaa, lathargy, and poor appatlta la all of tha anliaala.
At autopay tha livar aarlad from a straw oolor to dar\t rdd in diffarant
lsoara. Tha lntaatlnal traot waa lrrltatad with muodp pif aant in aoaaral
instanooa.
/" . \
MONS 061765