Document Xz54K8m5wV4rp8ZEkLQMdLMYg
AR1L-053%
TOXICITY TO AQUATIC PLANTS (E.G., ALGAE)
TEST SUBSTANCE
Identity:
aPlesroflbuoeroroecfteyrrlesdultfoonaasteF,ludoirdoealckyyildaimmemtohnyilaummmodneriiuvmatsiavlet.;
may. [1-
D1,e1c:a2m22i,n3i,u3,m4,,4N,-5d,e5c,6y,l6-.N7,.N7-,d8i,m8e8t-hhyelp-t,adsaelctawfiltuhoro-1-
octanesulfonic acid (1:1), CAS # 251099-16-8]
RL-e1m4a3r9k4s:referTrehdet3o Mbyprtohedutcetsitolnablootrantuomrybears
was Lot P3025.
1. The test sample The sample was
is
linabwealteedrF(-a1p1p6r1o5x,imLaottel1y. 3T0h-e40te%sttessatmspulbestisanacem,ix6tu0r-e70of%twhaeteters,tasnudbs0t-a5n%ce
oTfheretseisdtuaslapmeprlfeluaoprpoechaermsictaolbse).aA2ll-pvhaalsueesdirseppeorrstieodnr(ecllaetaertloitqhuiids mwiitxhture.
opaque solid) were made to
which adjust
rapidly for the
sacetpuaarlatceosncaefntterraatgiiotantioofn.theNtoesctaslucublsattainocnse
in
the test sample.
METHOD
_
Method: OECD 201
Test: Static acute
GLP: No
Date exposure completed: 1/11/97)
1/17/97
(Repeat of eariier study completed
Species: Source:
OrSiegliennalalsytfrruommcTahpreiAcmorenruitcuamn
Type
Culture
Collection
(ATCC),
Strain 22662.
Element basis: Growth Analytical monitoring:
rate and Nominal
number of cells/mL. concentrations only.
pH, conductivity,
temperature were monitored.
Exposure period: 96-hours
Start date: 1/13/97
End Test
doartgea:ni1/s1m7s/9l7aboratory
culture:
Algae cultures were growing in
OECD-recommended culture 4 days before test initiation.
medium
at
AScl
Corporation,
Duluth,
MN
for
Test Conditions: TDielsuttitoenmpweartaetrusroeurrcaen:ge:Th2e4.al6g--al2m5e.6diCum was prepared to OECD nruetcrioemntmesntodcekds c(oinnccleundtirnagtiNoanzsEDbyTAs)p.ikiTnhgedemieodniizuemdwwaastesrtewriitlhized
04254
via 0.22 pm filtration prior to use in the test. The pH of the synthetic algal medium at test initiation was 7.95.
Stock and test solution preparation: Water accommodated fractions. Test solutions were prepared for each test concentration by mass addition of test substance in 500 mL of algal medium. The solutions were vigorously stirred for 21-hour (vortex 1/2 solution depth). The aqueous phase was pipetted from the vessels at middepth after the solutions had settled for 1-hour. Exposure vessels: Sterile 250 mL borosilicate Erlenmeyer flasks plugged with foam stoppers containing 100 mL of test solution. Agitation: Shaken continuously at 100 rpm Number of replicates: Six for control, three per test substance loading Initial algal cell loading: 1.2 X 10 cells/mL Number of concentrations: Five plus a negative control Water chemistry:
pH range (0 - 96 hours) 7.95-943 (control exposure)
Conduct7i.v9i1t-y7r.a7n8ge(4(000--m9g6/Lhoeuxrpso)sure) 134 - 150 pmhos/cm (control exposure) 154 -- 166 pymhos/cm (400 mg/L exposure)
Test temperature range (0 96 hours) 24.6-256C
Light levels: (0 - 96 hours) 730- 750 ft-c from continuous coolwhite fluorescent lighting
Remarks: Inconsistent growth inhibition at increasing exposure concentrations was seen when the study was first run using exposure concentrations from 5.2 to 40 mg/L. In this first study, the 48, 72, and 96hour E,Ls (growth rate) could not be calculated, because less than 50% growth inhibition was seen at all exposure concentrations. The study was repeated at higher test substance concentrations. In the second study, complete inhibition was seen at every exposure concentration loading except the lowest two concentrations, 86.4 (growth rate) and 51.8 mg/L (cell density, growth rate). As a result, the 48, 72, and 96-hour EL (cell density) could not be calculated and the Ls at each time period was. slightly higher than the lowest exposure concentration.
RESULTS
oo
Nominal concentrations: Bk control, 51.8, 86.4, 144, 240, 400 mg/L. Element value: 24-hour EL (cell density) = 55.8 (50.2 - 62.1) mg/L
04255
24-hour 48-hour
E.Lsy ELso
(growth rate) (cell density)
= =
57.7 <51.8
(54.2-61.5) mg/L mg/L (CI not calculable)
4782--hhoouurr EE.LLsso:((ceglrlowdetnhsirattye))==5<45.18.8(5m0g./4L-5(9C.I6)nomtg/cLalculable)
72-hour
96-hour
E,Lsy
EL
(growth
(cell dens
rate) = 61.4
ity) = <51.8
(68.2-64.9) mg/L
mg/L (Cl not calc
ula
ble
)
86-hour
96-hour
E/Lso (growth rate) =
NOEL (cell density):
64.6 (62.5-66.9) mg/L.
7.7 (7.5-8.1) mg/L*
96-hour NOEL (growth rate): 29.3 (27.7-31.6) mg/L*
"NOEL values are approximations using the IC1o as the endpoint
All element values based on nominal concentrations.
vStaaltuiesstiwcearl emecatlhcoudlsat:edCeulslindgenasnitiEexsc,elgrsopwrtehadrsahteeesta.ndThpeerTcreintmmienhdibition
(`VSepresairomnan2.-0K1a,rbUeSrEmPeAt-hEoRdL,anDdultuhteh,InhMiNbi)tiwoenrCeonucseendtrfaotriEoLn
(ICp) approach and NOEL.
determinations.
Control response: satisfactory
Biological observations after 96-hours:
ConcnoTensoat"ton, [[omfiCail pr [iCiosniaantvyia|GeroiwtsnRavtis
[okcomn |amom|.|| [se Leonon| wo | a [olen Loe[a]
20 ra[e o |oo 0|
wo | rom | aw | aw |
Observations: means of direct
Algal cell counts in microscopic counts
each test vessel were determined with an Improved Newbauer
by
hemacytometer.
CONCLUSIONS dTehteertemsitnsedamupslieng96t-whoocuarlcEuLlsaotiionntemrevatlhofodrs.SelBeynacesltlrduemnscialyp,riict owransut<u5m1w.8as
604256
mg/L and by growth rate 64.6 (62.5 -- 66.9) mg/L. The 96-hour NOEL was determined to be 7.7 mg/L using cell density and 29.3 mg using growth rate. `Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kiimisch ranking 3. The study lacks analytical measurement of test substance concentrations in the test solutions and sample purity is not sufficiently characterized. Accurate ELsy's could not be calculated at each observation period due to the almost complete inhibition of cell growth at all exposure concentrations. Additionally, data is for a mixture: and toxicity cannot be positively attributed to didecyldimethylammonium Perfluorooctylsulfonate salt alone. Report quality: Two definitive studies were conducted on this test substance at the same laboratory, in the same time period. A careful examination of the raw data revealed several errors during report preparation. These are:
1. The "study completed" dates printed on the report covers (January 29, 1997 and February 21, 1997) are switched with each other. The sections under 1.0 General Information" for "Definitive Test Dates" are correct for each report, however.
2. The discussion under section "3.0 Test Methods" for "Test loadings" are switched. However, the test concentrations listed in this section are correct in each report
3. The algicidalialgistatic determination test was only conducted after the second study. The discussion under "4.0 Results" in the report with the cover reading January 29, 1997 should not have been included. No evidence was seen in the raw data that the algicidallalgistatic determination was conducted during the first study. The raw data from the second study revealed that the test substance was, in fact, algicidal.
REFERENCES This study was conducted at AScl Corporation, Environmental Testing Division, Duluth, MN at the request of the 3M Company. OTHER Last changed: 5/24/00
04257
ESrevlir"CnAoSmrEepsSoarlautToan1/n0A1Sge3lD01Di0iu.it0o6an8
STUDYTITLE
GROWTH
INHIGBRIETIEONN
OF ALGA
1(/S3e0l3e5n'astrum
capricornutur)
DATASTANDARD
OECD GUIDELINE 201
STUDYCOMPLETED
JANUARY 29, 1997
TESTING FACILITY
EAnSveilroCnomrepnotraaltTieosnt/iAnSgcID-iDvuilsuitohn 444D4uluAtihr,paMrkNBou5l5e8v1a1rd
Tel. Fax
No. No.
((221188))
772222--24509420
STUDYIDENTIFICATIONNUMBERS
AScl Study IDF 5010-068 3M Company Lab Request# P3025
SSppoonnssoorr: Su3dMyC1o0m8paP3n0y25
1
C04258
EAaSveiloAnCsmoeernpioSartlautdiTyosn1i/0nA4Sgc3lD0-1Diu0lo.u0tn6h
1.0 GENERAL INFORMATION
| study Tite
| i i Data Standard
Sponsor
I Sponsor's Representative
| Sponsor's Lab Request
`Testing Facility
1
| ombion Principal Investigator
`Testing Facility Director
Definitive Test Dates
| Data Validation and Report Review
Retention of Raw Data and Final Report Location of Raw Data and Final Report
Growth Inhibition of F355
|
Selenastrum
i
capricornutum).
1
OECD Guideline 201.
Il
S3eMrviEcnevsi,roBunimlednitnagl 2T-e3cEh-n0o9l,og9y35anBdusShafAevteynue,
St. Paul, MN 55133; Tel No. (612) 778-7452.
SanudsaSnafBeetaychS,er3viMcesE,nvBiuriolndmienngt2a-l36T-e0c9h,no9l3o5gy
Bush Avenue, St. Paul, MN 55133; Tel No. (612) 778-7452.
P3025.
AScl Corporation/AScl-Duluth Environmental |
Testing Division, 4444 Airpark Boulevard, i
Duluth, MN 55811; Tel. No. (218) 722-4040. |f
amo Todd McGuire
||
Donald Mount
January 13-17, 1997.
Report Signature:
Ho
"Two years from the date study is completed.
AScl Corporation/AScl-Duluth Environmental
Testing Division, 4444 Airpark Boulevard,
Duluth, MN 55811; Tel. No. (218) 722-4040.
SSppoonnssoorr: SIyM C1o0m4pPa2n0y25
2
C0459
2.0 OBJECTIVE(S)
EAnsveirCooarmpeonresiToens/iAnSgelDDiuvlisuotnh Se Stay 103 010.068
|
To determine the 4-d median effect loading (NOEL) of the test substance, ~ #365
(ELgq)
and
the
4-d
no
observable effect loading for green alga (Selenastrum
1 |
capricornutum) under static test conditions.
This study was conducted according to OECD Guideline 201 (OECD 1993). Test solution
preparation was water-accommodated fraction (WAF) per Girling & Whale 1994.
3.0 TEST METHODS
| Test Substance
fl
I
{
`Test Organisms
| Properties: {3035
, two phase liquid, specific
gravity ca. 1.2 (water = 1), stable, and insoluble in water.
Test loadings: 0 (algal medium control), 51.8, 86.4, 144, 240, and 400 |
mg/L. Test loading range was Each test loading was prepared
determined from by mass addition
range-finding test of test substance
results. to S00-ml
aliquots of algal medium. Test substance was thoroughly homogenized
before each mass was determined. Each solution was vigorously stirred
(vortex 1/2 of flasks' depth) for 21 hours to obtain WAF's.
obtained from flasks by pipetting from mid-depth.
Test solution
|
Source: ATCC 22662
1
Inoculum preparation: Algal stock was subcultured in OECD recommended medium for 4 days before est initiation.
Algal Medium
|
Use in test: Stock culture was diluted and spiked into test solutions to
obtain an initial cell density of 1.0 X 10% cells per ml (1.2 X 10% actual concentration).
Preparation: Deionized water was spiked with nutrient stocks (including |
Na,EDTA) to OECD recommended concentrations. The most recent chemical analysis for deionized water is in Raw Data Package.
SSppoonnssoorr: St3uMdy CDo8mpFaRnGyES
Characteristics: At test time, the medium had a PH of 7.95. Sterilized via 0.22 filtration prior to use in the test.
3
04280
[| CEhxpaomsbuerres || |I
| incubation
l|
|
i Endpoint lCalculations
EAnvSir"oCAaosmreeponSraualtdTioxn0i/nAgSe5tD0D1i0uv0aa6n8
Type:
foam
stSotpepreirlsi.zed
250-ml
borosilicate-glass
Erlenmeyer
flasks
sealed
with
|i
rUespelicinatteessta:nd1t0h0remelroepflailcgataelsmpeedrituemst osrubtsetstanscoelultoiaodni/ncghawmebreer.empSlixoyceodn.trol | wDuerrienmgatdhee. test, kept stoppered, except when experimental observations
Duration: 4 days.
l
Dfaluiolryespcheonttoplearmiposd.: TCeosnttcihnaumobuesrast 7he4l0d fotn-csahndalkeesrutsaibnlge sceotola-twh1i0t0erpm. |
Temperature: 25.0 + 2C.
|
IBimoplroogivceadl:NeDuabialvyercelhlemcoocuynttsomuestienrg.direct microscopic enumeration via
aWnadtetremcpheermaitsutrrey,: ((2)1)daAitlyt-estteimnpietriaattiuorne.andAltlerdmeitneartmiionna-tipoHn,s cwoenrdeucmtiavdiety, |
according to APHA methods (1995).
|
a1v+,er2a-g,e3s,peacinfdic4-gdroEwLtshy'rsa,teasn(d4)4.d NOEL based on both cell counts and
SSppoonnssoorr: St3uMdy CIoDmFpFa0ny25
4
94261
EAnSvcitr"oACaSmoeelmntSpauol rdTea1si0io4ngn0/D1DAi0uSv.tea0ln6
4.0 RESULTS
|
est Substance
in Tables 1, 2,
Toxicity
and 3)
(Pertinent
data
are
wCe1l-ldCoELugnyt:1-547E.L7ymg;g/5L5.(584.m2g-/61L.5()5.0.2.62.1), |
|
1Ce2lldCoELugnys;2-5d4E.8Lm:g/L<5(15.0.84-m5g9./6L)..
|
I|
#Ce3lldCoELusnyt:3-6d1E.4Lm:g/L<5(15.8.82-m6g4./9L)..
|
nCe4l-ldCoEuLns;4-6d4E.6Lm:g/L<5(16.2.85-m6g6./9L)..
|
|
|
|
I|
|
I Test Conditions.
Cell Count 4-d NOEL: 7.7 mg/L (7.58.1). |
# 4-d NOEL: 29.3 mg/L(27.7-31.6).
NICOELasvtahleueesndaproeinatp.proximations using the |
Effect of test substance was determined to be
algistatic based on the results ofa post-
definitive test exposure.
1
Temperature (C): 24.6-25.6.
L
_
_
Light intensity fi-cdls: 730-750.
|
PH: 7789.43.
|Specific conductivity (wmhos/cm): 134-166. a
SSppoonnssoarr: StIuMdyC1o0m1pPa3n0y25
s
04262
5.0 TEST QA/QC
EAnSveilroACnSmcoelrntSpatoludrTyeas1ti0oo4nng5/O0Ona1uv0Ssl.eiu0ot6g
Algal counts in control must increase by at I least a factor of 16 during the first 3-d of the | exposure.
Test duration mustb4ed.
_
During the first 3-d of the test controlalgal counts increased by a factor of approximately 191.
test durationwas 4-d.
]
6.0 REFERENCES
{ WAamteerricaannd PWuabsltiecwaHetaelrt.h AAPssHoAc,iatWiaosnhi(nAgPtHoAn),.D.19C9.5. Standard Methods for the Examination of |
| DGiertleirnmgi,nAi.nEg.,theG.AFq.uaWthiaclTeo,xiacnidtyDo.fM.PMoo.rlyAdWeamtae.r-S1o9l9u4b.leA CGoumipdelleixneMiSxutpuprleesmeUnstinfgorWater- 1
Accommodated Fractions. Chemosphere 29(12):2645-2649.
|
|
TOersgtainnigzaotfioCnhefmoircaElcso.noOmEicCDCooPpuebrliactaitoinonanIdnfDoervmaetlioopnmeCenntte(rO,ECWaDs)h.in1g9t9o3n., ODE.CC.D
Guidelines
for
Hamilton, M.A., R.C.
for Estimating Median
Russo, and R.V. Thurston, 1977.
Lethal Concentrations in Toxicity
Trimmed
Bioassays.
Spearman-Karber Method
Environ. Sci. Technol.
11(7): 714-719; Correction 12 (4): 417 (1978).
|
US EPA. 1993. A
Concentration (ICp)
Linear Interpolation Method for Sublethal Toxicity:
Approach (Version 2.0). Environmental Research
The Inhibition
Laboratory, Duluth,
|
LMinnesota.
SSppoonnssoorr: Si3udMy C1o0m4pPa3n0y25
6
94283
rin
--_-- TABLE 1.
ELgy'S, 95% Confidence Test with 305
Intervals,
and
4-d
NOEL'S
from
Algal
Growth
Inhibition
|I wo =F zug 95%Confidence {
-- orca[[E m ome ye ] T I n -- n T[ eo cc0ae 2a62nr .n1 | ]|
L
Gem |sa]
i se ET Ere
-- gorgeI s [mm
me Ten] sr][ses -- |
[eenmT Tm eee ee]
04264
stponmeinrsrfloee
= -- 7 1 rr TABLE 2.
Cells per ml and Percentage Difference capricornutum Exposed to psc $
from
Mean
Control
Value
for
Selenastrum
TotSLolwing we [TeTT
| cone 5;: ::
:;:5:
33H 3
F2mA FS
4aaa|
H
x
ES]
sia
ve | :x
eso :;
:
:
who n;
|
w25o
;
i
5a o0n] i
eo x3
|
nce 5:
|
sim :i
|
ad2: m
|
mite
[
c
o
2
3
:x5Mean eo |
wot 28;0.3 (:94)
|
1.3 .2:(96) wim |
2u.0a;:(:t99e) |
io:_ :do 1
{
x we:Bn
|
: st2; m |
: or.1 m |
: ol52en |
: ur2 2 d
L
wex::a | oco;;w | os0;m | esso:to | ors:di 1
* Pua Vis = 5 Dio fom Cos Mes Vas
4Me A Com = 12 if
a Spr 3 Com
.
C04265
EAnSveilroCnomrepnotraaltiToens/tAiSngelDDiuvilsuitohn ASC Study 104 5010.68
TABLE 3. AVavleureagfeorSpSeecliefniacsGtrruomwctahprRiactoersnu(4t)umanEdxpPoesrecdenttoag3e0D3if5ference from Mean Control
r| eTest osding bf
TT
I| Control ]
{|| L
lee [-- TE AeeeeE mcemmen T
B*c~ Tw LL7e6z4 = 1L770s1
| t17o55s 1788
-- s13a60 | es
5E F
1v4a2r7
LL6e5n7
Le
L701
11775055 L788
15s5a8 L566
Mean
169 0)
166 0)
1s 0
1590)
5x c
0L1a6r
o1.s06s0
0511
0552
1Li2s5s% 11m
11320756 1.269
Mean
Loz (7p 0945 (44)
1225 (30)
1284 (19)
xB c
oom
o00m91
o
0255
00016710 305
20022496 0.128
Mean
1386 (100) 0040 (8)
01m (30)
onsen |
xB c
osot 1:2
o25n5 0
0006611 0305
oo o | 0.128
Maan
0.182 (100) 0091 (100) 0.16 (95) 0.04 (10)
xB c
oos1mt 0182
oo0n0 o
S010700 o
o0.o2k8 os
Mean
0.080 (59) 0260 (100) 0061 (100) 0087 (54)
x5
oo
c
o
o3)
2006
oois
o
0
006
Mean
0.000 (100) 0.6 (100 2.462 (100) 0.35 (10)
"4 = In Celi per mit In Col wo
Parethetic Values = % Difference from Conteol Mean Value #04 Mean Algal Count = 1.2X 10%
SSppoonnssoorr: Si3uMdyC1o0m4pPa2n0y25
5
04266