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AR 226 _ 0910 ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-85, o as a component of FX-3103. Q"Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample nt information indicates it is a mixture of 0.96% PFOS, is FX-3103, 3%. Curre 20% diethylene glycol butyl ether, 50.56% water, 15% ethylene glycol, 4.32% `sodium octyl sulfate, 8.0% polyoxyethylene monooctylphenyl ether, 2.5% propane sultone foamer, 0.5% sodium nitrate, 0.06% sodium lauryl sulfate, and 0.1% benzotriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not.accurately reflect the toxicityofthe fluorochemical component of the test sample. METHOD: Method: Not Type: Acute given. static-renewal range-finder GLP: No `Year completed: 1985 Species: Supplier: Pimephales promelas Commerical Supplier, Brady, NE. Analytical monitoring: Temperature, pH, and dissolved oxygen. Exposure period: 96-hours `Statistical methods: LCs; values were calculated using logistic regression methods (probit analysis). Test fish age: Juveniles Length and weight: Average length = 41 mm Average weight = 0.50 g (wet) Loading: 0.67 gi Pretreatment: 25 mg/L Tetracycline HCI Test conditions: Dilution water: Carbon-filtered well water Dilution water chemistry: Not given. Lighting: Not given. Stock and test solution tothe dilution water on a preparation: The test weight/volume basis. material was added directly = Concentrations dosing rate: Solution renewal during the study. Stabilityofthe test chemical solutions: Notnoted. Exposure vessels: 4L glass beakers containing 3 Ltest solution. Number of replicates: 1 for exposure concentrations, 2 for blank control. Number of fish per replicate: 4. 002961 WNautmebrecrhoefmicsotnrcyendturraitnigontsh:e five plus study: a blank control pH range (0-96 hours): 77..6777..88 ((c1o0n0tr0omlge/xLpoesxuproes)ure) TDeismspoelrvaetduroexyrgaennger(a0n-g9e6 (h0o-u9r6s)h:our1s9):20C 75..19-99..33 mmggl/LL ((c1o0n0tr0omlge/xLpoesxuproes)ure) RESULTS Nominal concentrations: Blank control, 1, 10, 100, 500, and 1000 mg/L. Element values: 96-hour LCs =>1000 mg Element values based on nominal concentrations Remarks: Testing was conducted on a mixture as described in the Test tShuebsftluaonrcoechReemmicaarlkpsrfoipeoldr.tiTonhealvoanleu.es reportedapply to that mixture and not CONCLUSIONS The test sample 96-hour LCso for fatheadminnowwas determined to be >1000 mgiL. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY. Rneulmiabbeirliotfy:orKgiainmiissmchs eraxnpkoisnegd3.atTehaechstcuodnycewnatrsaatiroann.geT-hfeinrdeeriswaitlhaclkimoifted ochfatrhaectaemroizuanttioonfoffltuhoerotcehsetmsiacmalplper.opoTretsitonsoilnuttihoenssolluatcikona.nalytical confirmation REFERENCES TSth.isPasutlu,dyMNw,asLacbonRdeuqcuteesdtbnyutmhbee3rMC1C1o1m1p-a1n,y1,98E5n.vironmental Laboratory, OTHER = Last changed: 6/26/00 002962 I EH > AQUAETXIPCLTOROAXTIORCYIT(YRADNGAET-FAISNHDEERE) T w_ 2> p --/ "pe Test 5 Material Tested Ade mong HF tot st Crgantentin ryersSource Feng Ais De RecsSao aeen \201 54 RW, Sp _grans Avg. Size_5cn/Yo/. Organismsperdosloae din_ gFacK tor (7,4 7 a/L ish Disease Treatmmnt: 2a.5 st Pap ns NY Slaspry 2st SolutionVolute\*5Liters/All-Glass Tank Tank Dimensions: 4.1iter Pyrex? glass beaker imeStarted LAS purl. Date Started 22 [ABST DateCompleted = [8/85 EEPE FEE PEE ilution WaterCarbon-filteredwell water (Temp. /4 C., D.O. 2. =pm, p77 ete (95% Confidence Linits) 2/000 df lL Signal Tlaed = po Tvs] o bdzal 0blzd ) baz] alco =o] Tiled bell bpd bled] Hlsolo l dell belo bepdl 0 bos] Soo lxeeol bal bielsalbdool dso] [1 TT Ti T1117 11 1] 1 TteshtlsTap,teCt I|_T_7A 70 1 2T0T a 7 e|=JoScprR e2 e5e0r| oe Ln UTIELTS 100 odTone] leo 2 econdary Effects (otherthanMortality): E050, COPY AVAILAE = m=e MET Teper Reese ee = IST Et =] IN INS Te NS t Vv v vv |] I oo Juss ENVIRORENTAL LABORATORY : @2 mania1. soorecomu s oxn iery y of LACS SD 8 70 FaTREAD NOOH.von Srcmomscomrrrons!) JA E PERCE TORTI cmba(c =corpo 24m. wm. nm. sem. oe. coNTROL. o de lo 00 pC. deco o o ` o 0 o o o [= - Le,0 (95% comeromcE Can LINITS)(22)1000 oe 21000 Moov (1) TDeatatewnetree.avTehratgoersaofoftewontreyplifciasthivoenrsefuosredeacpherccoonncceennttrraattiioonn.wich tea (10) 18h per (16g else vere caestated ing Jogtact regseion nehode (Prob danlyee). 21000