Document XwDY6XrObE78q90pV1e840Ky

AR226-2827 Material Tested M, te l i a i Subm itted by: E. I. du Pont do Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine HASKELL LABORATORY REPORT NO. 323-70 [Organic Chemicals Department, Jackson Laboratory Haskell No.: 6338 0 tlier Codes ACUTE INHALATION TOXTCTTV gfocedure: For each exposure, six ChR-CD male rats, weighing 250 to 280 16-liter bell jar for four hours unless all died sooner in exp0Sed to the test ^ t e r i a l in a ot 1020 steel was heated to 130C (maximum temperature used in storage)'and' V p r a v e T v i Y ^ n m " 0801 ^ th-b tGSt material coupon through a stainless steel pneumatic spray nozzle with an outside tempeiaiure o f l s o ' c ^^ ^ eXPSl,lG Chamber test material were determined periodically by filtering a known v n i ,, L ,,c _15 ?' Chamber atmosphere concentrations of the pore size and determining the weight increase Tf thl p L L an7 b v 1T fl V atn,Sphere throu8h a I t e r paper of O.lu tion mer urements were made with a cascade impactor Gross and hi s t o r ^ t h !omatipraPh ^c method- The particle size distribu- -- "S d" 1'n8 ^ eXPUSUre 3nd tW ratS su rviving 14 days post-exposure. The other !u osur Results: '' "" ' ~ : ------------------------- -------- -----exPosul e. Exp. No. 1 2 Anal. Cone. Cmg/L) 1.8 4.1 Particle Mass Median Diameter (p) and __ Dispersion Cali 3.8 t 2.4 Mortality Ratio 0/6 1/6 Clinical Signs During Exposure Irregular respiration, red ears Irregular respiration, paleness; one out of six rats had convulsions, death after second hour Post-Exposure Initial weight loss followed by normal weight gain 10-14/:, initial weight loss, one rat lost 204 of initial body weight by second day; 3 >7 6/ 6 Irregular respiration, pale ears;, one normal weight gain followed rat had convulsions, deaths occurred between 2^,- and 3^ hours of exposure The generated aerosi I h * 8" " , U 'd " r 0 S l f 0 m e i * |," - U k * " " U a l on t h . v . n . o f the b e l l j a r and made i t d i f f i c u l t to o b , o w e the ra ta . Company Sanfiized. Does not contain T G C A CBS 2 Pathology: Two rats that died during exposure to greater than 7 mg/L of the test material had acute pulmonary hemorrhage and edema. Two rats, sacrificed 14 days post-exposure to 1.8 mg/L, had lymphoid hyperplasia of the spleen, edema, and tracheitis; one rat also had a focal area of hemorrhage in one lobe of lung. Summary: The Approximate Lethal Concentration (ALC) of] Histopathologic results showed acute pulmonary edema anahemo r r h a g e . is 4.1 mg/L for a four-hour inhalation exposure. is 0.43 mg/L (Haske U No. 5904 -- ^ 4 and the ALC tor .08 mg/L (Haskell No. 6047 four-hour rat exposures, therefore less toxic than either Haskell Nos, 3904 or 6047 under the test conditions. t Tissues examined include: lung, trachea, kidney, liver, spleen, brain, adrenal, testes, stomach, heart, eye, ear, thymus, duodenum, and bone marrow. ' Geometric Standard Deviation. Experimental Work by: _ Q Q Figen i0_._jTaLyf-i^i * Assistant Toxicologist Inhalation Toxicology Section F T :DAS/pgh bate: Juiv 31. 1970 Approved b y : l ________ Dwrothy A, Snee Toxicologist Inhalation Toxicology Section f jLl L l J ; / ' ' / John A . Zapp, /Jr, Director Company Sanitized. Does not contain TSCA CBi