Document XoxZ29O8brwO3yGKEBr02kGJ

AR286_ 1272 February 7, 008 DRAFT Stud Pan: impart Ass of Poucs TC(ho5emplliooedwibnySMeimpgrpe3r0oip0oseed teytpela boyrTtReEamyesmobf RrOoAsin f1oriyf,s loamerrbaseed prproocducts Product Types: acrylate and urethane polymers (1 product formulations) DTheapmroposaedotstudy i daesiganeldyooderceonnicwhsetohfher PoFOeginertetsein scTho product,endoiins PROPOSED APPROACH An LCIMSIMS methodology will be usd for these nays having lower limitofquanios: tion of | ppm with a relative standard deviationof 20%. "The studywillbeconductedasGLP std. -Assumes that extraction procedures submitted here will be successful for all 11 product formulations. Procedure | assumes that to a known amount ofemulsion, methanol is added, shaken for a periodof time, and centrifuged at 3000 rpm for 15 minutes. The supernate is diluted | 10 100x with water, filtered and analyzed by LC/MS/MS. cFonnefceusssaerdy,nmdetdheacnaonltedaTdhde soutpheerasr iierodm hwiethfrtsteexrtrac1t1i1on0,%soBnoiocss,snd analyzed by LC/MS/MS. PV"irAcodhcdoeddm:uerteShaonhoile1 he emulsion ad collect plymer us preiptic Dry h -After efpirletecriipngittahteesaupelronwatparnets,suarnea,lypzaecbkynLtCh/eMtSe/M,Sad weigh -Using 140 mLof methanol, extract for 8 hours ~Cool, and using methanol, bring extraction fluid to 200 mL and analyze by LC/MS/MS. Internal standard is used throughout. Procedure is time consuming. r"Epxrsocntcihosn frfeienrchits shehtooutbbeeeavdadleudatesoammaptlleeast two or hee product groups sing bth Can est blankconirl be abaindgeneratd in abratory? No SBnadocnidoaroecnoverpipersesshcohud be cvaluted a 1 ppm, 10 pp snd 100 ppm. Ansys willuse DRAFT | 5 33= 2 22 Gr ~ c ~ TELOMER RESEARCH PROGRAM Triplicate samples analyzed per formulation "LC/MS/MS method developed for however, product composition may arretqiuclierse smheotuhloddberea-dwoarpkt.ed for analyses of products Final report willbe requested; data summary willbe acceptable for May 2003 submission. DRAFT 2