Document XY1vBqb1B0dLRXdveeOR2DV4
AA S & G -08/
TOXICITY TO AQUATIC PLANTS
TEST SUBSTANCE
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206CE. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was 502. The test sample is FC206CE (2/16/89). It's purity was not sufficiently characterized, though current information indicates it is a mixture of 0.92% PFOS, 15% diethylene glycol butyl ether, 76.09% water, 4% urea, 1.28% Sultone foamer, 1.55% sodium octyl sulfate, 1.05% polyoxyethylene monooctylphenyl ether, 0.06% sodium lauryl sulfate, and 0.05% tolytriazole.
The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect die toxicity o f the fluorochemical component o f the test sample.
METHOD:_________________________________________________________
Method: OECD 201 and ISO /DIS 8692 Type: Acute Static GLP: No Year study perform ed: 1989 Species: Selenastmm capricomutum Source: Originally obtained from ATCC, Rockville, MD, maintained in culture medium at 3M Environmental Laboratory, St. Paul, MN. Elem ent basis: Algal cell counts (cells/ml). Exposure period: 96 hours Statistical Methods: Not given. Analytical m onitoring: pH and temperature.
Test Conditions:
Algal Nutrient Medium : Nutrient medium per OECD 201 and ISO/DIS 8692. This nutrient medium provided all mineral nutrients essential for algal growth and also served as the diluent for all algal operations. The pH of this synthetic algal medium was 7.6 adjusted to 8.3. One hundred mL of this pHadjusted nutrient concentrate solution was added to 900 mL of Millipore MilliQTM water. Final pH 8.3.
Stock and test solution preparation: A 1000 mg/L stock solution was prepared by diluting 1000 mg of test substance in 900 mL of Millipore MilliQTM water then adding 100 mL of the pH-adjusted nutrient concentrate solution. Final pH of this stock solution was 7.9. Aliquots were then added directly to dilution water in test vessels to create test solutions.
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Exposure vessels: 250 mL Erlenmeyer flasks containing 100 mL test solution and capped with stainless steel closures.
A gitation: Shaken continuously at ~100 rpm. Num ber o f replicates: 3 for blank controls and 2 for test material treatments Initial cell loading: 1.0 x 104 cells/mL Num ber o f concentrations: five plus a blank control Lighting: 400 foot-candles from continuous cool-white fluorescent lighting. W ater Chemistry:
pH range (0-96 hours): 8.3 - 9.4 (control exposure) 6.8 - 7.9 (1000 mg/L exposure)
Test tem perature (0-96 hours): 24 C (incubator)
RESULTS
Nom inal concentrations: Bk control, 100,180, 320, 560, and 1000 mg/L
A lgal Growth Response at 96 Hours
Nom inal C o n e ., m g/L Bk. Control
100 180 320 560 1000
Avg. Growth Rate
1.3853 1.3461 1.2933 1.0908 1.0378 0.8193
% Change in Growth R a te
--
-2.8 -6.6 -21 -25 -41
Element values are based on nominal concentrations.
R eversibility o f G rowth Inhibition: Not given
Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
C O N C L U S IO N S _______________________________________________
FC-206CE exhibits a 96-hour percent change in growth rate compared to the control of -4 1 % at the 1000 mg/L treatment.
Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
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DATA QUALITY Reliability: Klimisch ranking 2. This study meets the criteria for quality testing. However, the study lacks information on purity of the test substance and actual measurements of the amount of fluorochemical proportion in the solution. REFERENCES____________________________________________________ Test was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number G 1358-2,1989. OTHER____________________________________ ____ _________________ Last changed: 6/26/00
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ENVIRONMENTAL LABORATORY STANDARD NUTRIENT MEDIUM FOR FRESHWATER ALGAE
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NUTRIENT
CONCENTRATION
FINAL CONCENTRATION
IN STOCK SOLUTION
IN TEST SOLUTION
STOCK SOLUTION Is MACRONUTRIENTS
NH .CI
MgCl,.6H,0 CaCl,.2H;0
MgSO.7H0 KH2p4
1.5 g/L
1.2 g/L 1.8 g/L
1.5 g/L 0.16 g/L
15 mg/L
12 mg/L 18 mg/L 15 mg/L
1.6 mg/L
< SOLUTION 2s Fe-EDTA
FeCl,.6H,0 Na2EDTA.2H20
80 mg/L 100 mg/L
- 80 ug/L 100 ug/L
: SOLUTION 3s TRACE ELEMENTS
H BO
MrtCl,.4H,0 2nd, CoCl,.6H,0 CuCl,.2H,0 Na2M04.2H20
185 mg/L
415 mg/L 3 mg/L 1.5 mg/L 0.01 mg/L 7 mg/L
185 ug/L
415 ug/L 3 ug/L 1.5 ug/L 0.01 ug/L 7 ug/L
STOCK SOLUTION 4s NaHCO-
NaHCO,
50 g/L
50 mg/L
Sterilize stock solutions 1-4 by membrane filtration (mean pore diameter 0.2 urn).
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SOLUTION 5: NUTRIENT CONCENTRATE (Prepare freshly before each test.)
` v-/ : Add 100:raLiof STOCK SOLUTION 1; , - l1O0 -mL'of STOCK SOLUTION 2:; ' :ii>.r .:l0!mL|of STOCK SOLUTION 3;
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LGL8NUTRIENT* MEDIUM Jbjl.l
V J&H'Sph' ' ' is v
^ ^ ^ 'f l O W i ^ f |the"pH-a^usted Nutrient 'Concentrate Solution'
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REFERENCES
;(1) S 0ECD?9 4' |Alga Growth Inhibition Test; 201.1ns Guidelines
00174
NH .Cl
MgCl,.6H,0 CaCl,.2H;o
MgSO.7h 1o k h 2p o 4
1.5 g/L
1.2 g/L 1.8 g/L
1.5 g/L 0.16 g/L
15 mg/L 12 mg/L 18 mg/L 15 mg/L
1.6 mg/L
STOCK SOLUTION 2; Fe-EDTA
FeCl-.6H_0 !Ja2EDTA.lH20
80 mg/L 100 mg/L
80 ug/L 100 ug/L
STOCK SOLUTION 3: TRACE ELEMENTS
H SO MnClf.4H-0 ZnClf a CoCl.6H-0 CuClf.2HO Na-MOO.,2H,0
185 mg/L
415 mg/L 3 mg/L
1.5 mg/L 0.01 mg/L
7 mg/L
185 ug/L 415 ug/L
3 ug/L
1.5 ug/L 0.01 ug/L 7 ug/L
STOCK SOLUTION 4; NaHCO-,
/ ' 'NaHCOj
50 g/L
50 mg/L *-
Sterilize stock solutions^l-4;by membrane filtration
;4v>'-V"ean p0r diame
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L? ?4 ;*4iSOLUTION'5: NUTRIENT'CONCENTRATE^ ''
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BEST COPY AVAILAbI | ;
ENVIRONMENTAL LABORATORY FRESHWATER ALGAE GROWTH INHIBITION TEST
ALGAL CELL COUNT, No./mL
HBCCYTCMETER METHOD* .
TEST D A Y .,, .,y
----------TEST TYPE
MATERIAL T E S T E D P S ^ / / 0 > / r y ) S(TT ,W >
ALGAL SPECIES: Green Algae, Selenastrun capricomutun
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TREATMENT
jS!SiU
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TOTAL OF 25 FIELDS COUNTED
No./nL
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ENVIRONMENTAL LABORATORY FRESHWATER ALGAE GROWTH INHIBITION TEST
ALGAL CELL COUNT, No./mL i H B 10CYT0METER MEIHOD* " t
TEST D A ^ L _ y
) TEST TYPE
MATERIAL TESTEDl / W P A Q & A //,,/ X V ) r>T. W
ALGAL SPECIES: Green Algae. Selenastrun capricornutun
A N A L Y S r l2 ^ ^ /^ ^ ;y>^ ^
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DATE.q ^ 6 ,? /? 9
TREATMENT
TOTAL OF 25 FIELDS COUNTED
X XT V czr^cc
,0a il
2,14
a
No./mL
2 ,5 4 0 i0 0 O Z.|?b<d0,000 >2,550,000
7,7 20 ,000
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pH
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BEST COPY AVAILABLE
001
. / JLab Request Mo
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Environmental Lab - Work Sheet
Page------- of
Sample Description
ALGAL ASSAY: BOTTLE TEST (AA:BT) COMPARISON OF GROWTH RATES
D ata:__ S .
MATERIAL T E S T E D > PP) {jJ/b/m ) l T ^ O Z ^
Analyst:
ALGAL SPECIES: Freshwater Green Algae. Selenastrum capricomutum
TREATMENT
CELL COUNT M-DAY
GROWTH R A f E ^ M -day
" '4 C H A N G E ^ ................... M -day
x i .i o n 1 '
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a itt .U O ? f t * A 2> -- *41 _______ f l > s \ 4 5 r __ __________________________________
( 1) S p e c ific Growth Rate
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I n Nx - I n N0
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