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AR Q26 - 0911 TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE Identity: ArefmeirxrteudretocaonstPaiFnOiSng,pFeCr-f9lu5o,roorocatsanaescuolmfopnoanteen,twhoifcFhX-m3a1y03a.lso(1b-e Octanesulfonic acid) (CAS # 2795-39-3). Remarks: is FX-3103, 3T%.heC3urMrepnrtodiuncftoiromnatliootnniunmdbiecartwesasit not is a noted. The test sample mixture of 0.96% PFOS, 2`S0o%diudimeotchtyyllesnuelfgaltyec,ol6.bu0t%ylpeotlhyeorx,y5et0h.y5l6e%newamtoenro,oc1t5y%lpehtehnyylleneethgelry,co2l.,54%.32% propane sultone foamer, 0.5% sodium nitrate, 0.06% sodium lauryl sulfate, and 0.1% benzotriazole. cTrhehfaelreafccottltelhorewiiztneogxdisccouintmycomefanttrrhyaetaipfopllnuisooerfsoctihomepamuimrciiatxlitecusro.emDpwaiottnhaeimnntacyoofmnptolhteetatecelcsyutrsaatmeplyle. METHOD: Method: Not given. Type: Acute static GYeLaPr:sNtuody performed: 1985 Species: Selenastrum capricomutum Source: Element Not given. basis: Algal cell counts (cells/mL). Exposure Statistical period: 96 Methods: hours. ICs values calculated using probit analysis or other Arneaglryestsiicoanl mmoodneiltso.ring: Not given. Test Conditions: Algal Nutrient Medium: Stock and test solution Not given. preparation: Not given. Exposure vessels: Not given. Agitation: Not given. NInuitmiablecrelolflroeapdliincga:te2s:.052x 10 cells/mL Number of concentrations: five plus a blank control Lighting: Not given. - Water Chemistry: Not given. RESULTS Nominal concentrations: Blank control, 100, 180, 320, 560, and 1000 mg/L. 002965 96-Hour Algal Growth Response Moan Cell-Count |_ Percent Inhibition 9 (cells/ml [Control | 0_[ 1,790,000 trro000 = |A | 1,080,000 [40| [80 | 7000 | [60 | 3tooo0 60 88 | | Element values: 96-hour ICso = 236 (19--3 273) mg/L. Element values are based on nominal concentrations. Reversibilityof Growth Inhibition: Not given. Remarks: Substance TReesmtianrgkwsafsielcd.ondTuhcetveadlouensarmeipxotrutreed as described applytothat in the Test mixture and not the fluorochemical proportion alone. CONCLUSIONS The test substance confidence interval oefxh1i9bi3ttsoa29763-hmogu/rL.ICso of 236 mg/L with a 95% Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY pr-------------- Rmeeltihaboidliftoyl:loKwieidm.isTchherraenkisinngo3c.haSrtauctdeyrilzaactkisodneosfcrtihpetitoesntasnudbsrteacnorcdesopufritthye, and lacks analytical confirmation of the amount of fiuorochemical proportion in the solution. REFERENCES TSth.isPasutlu,dyMNw,asLacbonRdeuqcuteesdtbnyutmhbee3rMC1C1o1m1p-a1n,y1,98E5n.vironmental Laboratory, OTHER oT Last changed: 6/26/00. 002966 | FRESHWATEENRVIARLGOANEMENGTRAOLWTHLABIONRHAITBOIRTYION TEST ALGAL CELL COUNT, no./ml HEMOCYTOMETER METHOD* Test wg west we XBL MATERIAL ste EXSIOSSh ALGAL SPECIES! sgnfae Solas aii WLLU=L we 2/25/85 BRTROG T a TOTAL OF 25 FIELDS COUNTED \80 M8 no. /ml 1,800,000 1,150,000 MEAN + SD 1,1q0,000 100wall =1 M8 2-1 Mo | 1 "1@0,000 1,110,000 1 410,000 T80wal 2 \=2 g S20 1,o10o0,000 1,080,000 1,000,000| X 1-3 2-2 ; "T2Z, . 20,!000 "120,000 H20,000 BOwall =k 2. . 2-4 20 005wal 220,'000, 300,000 &i0,000 ; 1-5 20 2-5 20 00,) 000 200,000 200,000 1 0.1 wm deep Hemocytometer; area of field 1 x 1and 0.1 mm deep. 002967 . Chui=1 TABLEEN1.VIROSNUMMEMNATRAYLOFLABAOCRUATTEORTYOXICITY oF TO FRESHWATER ALGAE : (seenastron capricorn2)) J -------- * Percent Inhibition in Standing cop (2) (mg/1) Chemical Concntration 4BC8ae-slHelrd-.Coounnt| CeBlals-edCouonnt9|6-Hr.CelBlaseDdryonWE. Control : - loo ! 80 Ho 220 bo So 83 ooo a . Ic,, 50 (95% Contidence Limits) mg/1() 23,(19%-2"42) Acceptability of Test obal Yeading 20,500cells [ud . The Selenastrum capricornutum control cultures have met the following quality criterias, - Exhibited log phase growth within HE hours (max. 48hr.) - cAetltlas/imnlead 96-he. standing crop of Axl cells/al (min. 10 Loqqhose rau at HB-Wrs, HHSccocellslud. (1) Data were averages of two replications for each test concentration. --_ (2) Based on cell-count (no./ml) and/or cell-dry weight (mg/l). (3) r1ce,jfevssailounesmwoedreelscaolfcuelxapteerdimeunstinagl dPartoab.it Analysis or other 002968