Document XRRZxonYbROEbpzZgzzmL7Y5B

AR226 0357 CHRONIC TOXICITY TO FISH TEST SUBSTANCE Identity: N-ethylperfluorooctane sulfonamidoethanol; may also be referred to as 1N,-1E,t2,F2O,S3,E34A,l4c,o5h,o5l,6o,r6,F7M.-73,482,82,.8-(h1e-ptOacdteacnaefsluulofron-Na-m(i2d-eh,ydNr-oextyheytlh-yl)-, CAS # 1691-99-2) Remarks: Material is an off-white, waxy solid of uncharacterized purity. The laboratory conducting the study refers to N-EtFOSE Alcohol as "78.01". METHOD: Method Followed: The methodology for the egg and fry exposure closely followed that presented in "Proposed recommended bioassay procedure for egg and fry stages of freshwater fish" (U.S. EPA, 1972). Type (test type): Flow-through GLP: No YSpeeacriesst/uSdtyrapienr/fSourpmpelide:r: 1978 Fathead minnow (Pimephales promelas) eggs from the brood Agency's EsntvoicrkoonfmtehnetaUl.SR.esEenavirrcohnmLeabnotraaltPorroyteicn tion Duluth, Minnesota. Analytical monitoring: Temperature, dissolved oxygen concentration, and pH were monitored daily, alternating between aquaria such that each aquarium was measured once each week. Remarks: One thousand mi water samples were taken from each aquarium at the initiation of the test (day 0), when hatching was completed (day 4), and weekly thereafter (days 11, 18, 25, and 32) for determination of N-EtFOSE `aAplpcroohxolimcaotnecleyntmraitdiwoan.y Samples were taken between the surface, with a beaker from a bottom and sides of point each aquarium, and stored in 1000 ml amber glass bottles with foil lined caps. All samples taken during the test were stored at room temperature and shipped on May 31, 1978 to the 3M Company. Exposure period: 4, 11, 18, 25, and 32 days `Statistical Methods: Means of measured biological parameters from duplicate aquaria were subjected to analysisof variance (Steel and Torrie, 1960, completely randomized block design, P=0.05). Data for percentage survival and 006388 percentage hatch were transformed to arc sin square root of percentage prior to analysis. Test Condition Remarks: Exposures were initiated within 48-hours after egg fertilization and continued through 30 days post-hatch. The diluent water was well water pumped 10 a concrete reservoir where it was aerated before flowing to the exposure system through aged PVC pipe. The pH oalfkatlhiinsiwtya,te2r6-r2a9ngmegd/LfraosmC7a.0C7O.;2;atnodtalspheacirfdincecsosn,d3u1c-t3a8ncmeg,/L14a9s-1C7a0CuOm;hos/om. Five nominal concentrations of N-EtFOSE Alcohol, ranging from 1.3-20 pg/L, `control water (well water), and control water containing solvent (Dimethyisulfoxide (DMSO) at 24 mg/L) were delivered to duplicate test aquaria. A 50 ml gas-tight syringe was used to deliver 46 yl of a 0.84 mg/ml stock solution of N-EtFOSE Alcohol in DMSO through a stainless steel needle and polyethylene tubing to the mixing chamber of the diluter. Each glass test aquarium measured 40 X 20 X 25 cm with a 19 cm high standpipe drain which maintained a constant test water volume of 15.2 liters. The diluter delivered 0.50 liters of test water to each aquarium 185 times per day, yielding a 90% test water replacement time of approximately hours. The aquaria rested in a water bath containing circulating water heated by immersion coil heater and regulated by a mercury column thermoregulator designed to maintain the test water temperature at 25C. Upon arrival at E G & G, Bionomics, the eggs were allowed to acclimate from 17.5C to the test temperature of 25C over a period of two hours. Sixty eggs `were then randomly distributed to each of 14 egg cups which were then dipped in 2 60 mg/l malachite green solution for 15 seconds to prevent fungal growth. One egg cup was then suspended in each of the 14 test aquaria. Egg incubation cups were glass jars (5 cm O.D., 8 cm high) with 40 mesh Nitex" screen bottoms. An egg cup rocker arm apparatus, as described by Mount (1968), was used to gently oscillate the egg cups in the test water. Dead eggs were removed and counted daily until hatching was completed. Percentage hatch was calculated based on the numberof live fry per aquarium after hatching was completed compared to the numobfeeggrs per aquarium (60) at the initiation of the exposure. To initiate the 30-day fry exposure, forty fry were randomly selected from each egg cup and transferred to the respective aquaria. Upon transferoffry to the aquaria, the fry were fed newly hatched San Francisco Bay variety brine shrimp naupli, ad libitum, three times daily throughout the exposure period. The aquaria were brushed and siphoned to remove excess 006389 food and fecal material three times per week. At 30 days post-hatch, percentage survival, mean total length, and mean wetweightwere determined for fry from each aquarium. The fry were measured individually to calculate a mean and standard deviation total length while each fry group (fry from one aquarium) was wet weighed to calculate a mean wet weight. A(2t0thueg/tLe)rmwienraetiporneosfetrhveedteisnt,1t0h%e fbruyfffreroemdtfhoermcoanltirnowlhainled tthhee fhriygfhrcoomntcheentortahteiron test aquaria were frozen. Ten formalin-preserved fry (5 from each replicate) from the control and the high concentration underwent histopathological examination o(fSeaetrTaanbslvee3r)s.e sTehcetiroenomafintihnegnparreessearnvdedcferpyhaalnidc ferxotzeennsifroynwoefrteheanlaatleyrzaledlianeta laterdate (by 3M Company) for N-EtFOSE Alcohol concentrations. RES! S The biological data generated in this study indicate that hatchabilityofeggs, and apdevrecresnetlaygeafsfuercvtievdala,tmaenaynNt-oEtatlFlOeSnEgtAhlacnohdolmecaonncewnettrawtieonitgeosfhtferdt.y were not Remarks: Water quality parameters measured during the egg and fry exposure exhibited litle variation between test days and test chambers. Dissolved oxygen ranged from 8.2-10.2 mg/L. Temperature ranged from 23.5-26.5C and pH ranged from 6.9-7.5. 006330 TABLE 1 PERCENTAGE HATCH OF EGGS, PERCENTAGE SURVIVAL, MEAN AND STANDARD DEVIATION TOTAL LENGTH, AND MEAN WET WEIGHT OF FATHEAD MINNOW (Pimephales promelas) FRY EXPOSED TO N-ETFOSE ALCOHOL FOR 30 DAYS POST-HATCH.. coNocmionaoln[|----Fes -- ____ oat ____30Days | (uo) honslength in eel 8[ 2 |e 5 [a[28g |]A= lL SSolvent |s 8A [TSeS3 [l&e[s H20(3S)]]&w4 | | L P=es 5x [T&ell&@[ilaagg]]&%|| LesA [T93 elel21a3) [%60 | L : [ess le &[lal[s &] "The 1000 ml water samples taken during the study (described in "Analytical Monitoring") were analyzed at 3M Company. Results indicated excellent recovery. See Table 2. 006391 TABLE 2 ANALYSES FOR N-ETFOSE ALCOHOL IN BIONOMICS SAMPLES TO VERIFY RECOVERY CAPABILITIES [c[o TTmpe TEn R] I Ass-- | GomTwiSA-- |-- i-- ] [_Ase2i | SoientContolA| <0.1 | [_EAS s6 m2 e2r -- | -- SowvetnaiCso-- nwolBT |-- --w <0-- 0]| [Asso ies 1iTion) | [As I ero-- ---- 125 -- 10.160)-- | [asst [2 15 i213 1 [assis 5 1 sas | [Assi Asa e T fo 1 s |_fAoszssis || CAs | 20 | (a) received broken in shipment (b) replaces original samples A5617; AS618 198206 TABLE 3 RESULTS OF HISTOPATHOLOGICAL EXAMINATION OF FRY OF FATHEAD MINNOW (PIMEPHALES PROMELAS) EXPOSED TO 20 pg/l OF N-ETFOSE ALCOHOL* Observ0ations ioNomaFlindings &/10 Liver Fatty Change 3/10 Gill Hyperplasia ; 10piGinleyype|rpasia a. Work performed under contract to EG & G Bionomics Laboratory' NliOstTeEd.: T"hOenloynltyhotsaesuiesscuheasnwgheiscohbwseerrveemdiwsseirneghoyrpecropnltaasiinaedofdgeimlolnasmetlalbalreepcihtahnelgieumaraend Cfahtayngcehs saeenonfgtrhoeeuivneerl. inThhoeasleicyhfahn.gesAuwetroesJuodfggedltsosbuoomwianismaolbsaenrdvecodnsnisstoevnetawlith ainhe.poTshtiesricorhacnogresawapsorpdroonboafbtlyeugsi tsphaecpeoor penelraton of he buffered ormain 1 006392 CONCLUSIONS `gTrhoewtNho, EhfifsetcotpaCtohnocleongtyraatnidonsu(rNviOvEalC)ofoFfaNt-hEeatdFOmSiEnnaolcwohfroylifsorgrheaattcehrabtihlatny,19.820.8 g/L measured (20 ug/L nominal), the highest concentration tested. DATA QUALITY. Reliability: Kiimisch ranking = 2. This study meets all the criteriafor quality testing, but has several deficiencies. It lacks information on purity of the test swuabsstnaontcde,onteheatprtohdeurcetpioorntleodt snoulumbbileirtyflriommitwfhoricthhettheesttessutbssatmapnclee.was taken, and REFERENCES This study was conducted by E G & G, Bionomics, Wareham, Massachusets, 1978 on the request of 3M Company. TReOse78a.r0c1h (RNep-oErTtF"OTSHEEAELFCFOEHCOTLS)OOFNCHOANTTCIHNABUIOLUISTYAQOUFEEOGUGSSEAXNPDOSURE pGrRomOeWlaTs)H."ANReDpoSrUtR#VIBVW-A7L8-O6F-1F9R5,Y EOFG F&AGT,HBEiAonDomMiIcsN,NAOqWuat(iPcimTeopxhiacloelsogy Laboratory, 790 Main Street, Wareham, Massachusetts, June 1978. RFeAsTeHaErcAhDRMepIoNrtNO"WSU(MPiMmAeRphYaOleFs HprIoSmTelOaPsA)TEHXOPLOOSGIECDATLOE7X8A.M01IN(ANT-EItOFNOOSEF AAqLuCatOiHcOTLo)xiFcoOlRog3y0LaDbAoYrSa.to"ryR,e7p9o0rM#taiBnW-S7tr8e-e6t-,1W9a5r,eEhaGm,& MGa,sBsiaocnhoumsiectsl,s, June 1978. 3FlMuoTreocchhneimciaclalRse,poPrrtojSeuctmmNaurmyb,er"A9na9l7y0t6ic1a2l6M4e3t,hRoedpoolrotgyNuamnbdeSrup0p0o8r,t"A,rtFhautre of Mendel, January 17, 1979 OTHER Submitter: Minnesota, 5531M33Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Last changed: 5/18/00 006393