Document XRR9dbL1mZZ3Y8eL5r9N6447K
Wildlife International, Ltd.
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Proiect Number 454A-107
PFOS: A FLOW -THROUGH LIFE-CYCLE TOXICITY TEST WITH
THE SALTWATER MYSID (Mysidopsis bahia)
ADDENDUM
WILDLIFE INTERNATIONAL, LTD. PROJECT NUMBER: 454A- 107 3M LAB REQUEST NO. U2723
U.S. Environmental Protection Agency Series 850 - Ecological Effects Test Guidelines
OPPTS Number 850.1350
This stud3,'was reported using a test substance purity, of 90.49%. The test substance characterization was revised on 9/7/00 and the purity was determined to be 86.9%. The Sponsor requested that the results be revised based on the most recent purity analyses. The results previously presented were simply corrected by the ratio of the two purities or a factor of 0.9603. No attempt was made to recalculate analytical standard concentrations or measured concentrations from original raw data. As such, minor errors due to rounding may be present. Attached is the revised surnmaB, page from the final report and the most recent certificate of analysis.
t
Wildlife International, Ltd.
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ProiectNumber 454A-107
REVISED STUDY SUMMARY BASED ON A PURITY OF 86.9%
SPONSOR: SPONSOR'S REPRESENTATIVE: LOCATIONOF STUDY, RAW DATA AND A COPY OF THE FINAL REPORT:
WILDLIFEINTERNATIONAL LTD. PROJECT NUMBER:
TEST SUBSTANCE:
STUDY: NOMINALTEST CONCENTRATIONS: MEAN MEASURED TEST CONCENTRATIONS"
TEST DATES:
LENGTH OF FIRBTGENERATIONEXPOSURE:
3M Corporation Susan A. Beach
Wildlife International, Ltd. Easton, Maryland 21601
454A-107
PFOS (Perfluorooctane Sulfonic Acid Potassium Salt) PFOS: A How-Through Life-Cycle Toxicity Test with the Saltwater Mysid (Mysidopsis bahia) Negative Control, 0.083, 0.16, 0.33, 0.66, 1.3 and 2.6 mg a.i./L Negative Control, 0.055, 0.12, 0.24, 0.53, 1.2 and 2.5 mg a.i./L Experimental Start (OECD) - May 26, 1999 Experimental Start (EPA) - June 16, 1999 Biological Termination - July 25, 1999 Experimental Termination - July 25, 1999
35 Days
TEST ORGANISM: SOURCE OF TEST ORGANISMS:
AGE OF TEST ORGANISMS:
Saltwater Mysid (Mysidopsis bahia)
Wildlife International,Ltd. Cultures
Easton, Maryland 21601 Juveniles <24 hours old
NOEC: LOEC: MATC:
0.24 mg a.i./L 0.53 nag a.i./L 0.36 rng a.i./L
Wildlife International, Ltd.
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CERTIFICATE OF ANALYSIS
Project Number454A-107
INTERIM CERTIFICATE OF ANAL YSIS
Centre
Revision 1(9/7/00) Analytical Laboratories COA Reference
3M Product: PFOS, Lot 217 Reference #: SD-018 86.9%
#: 023-018A
Test Name
Specifications
I
Purity I
_ _:i i/.i.v ii:i:_:.i :_J. !ii::-i _: ! _:i!:Ji::_::i:::.!i2!:i!:i_i::i::
Appearance
White Crystalline Powder
Result 86.9% Confomas
IdentificNatMionR Metals (ICP/MS)
2. Magnesium 3. Sodium 41.. CPoatlacsiusimum'-
5. Nickel
6. Iron 7. Manganese Total % Impurity (NMR) Total %Impurity (LC/MS) Total % Impurity (GC/MS) Related Compounds POAA
Residual Solvents (TGA)
Purity by DSC Inorganic Anions (IC)
1. Chloride 2. Fluoride 3. Bromide 4. Nitrate
5. Nitrite 6. Phosphate 7. Sulfate' Organic Acids s (IC)
:i iI i
! i'i
i
ill "
ii::i.}}i iii!iii!i.i:i:fii:i!;!-i:i!;!::!;!::i::i:!i::?i:i:ii!:_!:i?::)i!_!iii_:_i!:._:i_::::_i::i!:_i_i.:_::!._!!_i;_i_:i:_
Positive
!I II
:
i!
"
:
"
: ::::i:
2. 0.001 wt./wt.%
3. 1.439 _t./wt.% 41.. 06..080459w_t.A./,w_.t%.%
5. <o.ool
6. 0.005,,./wt% !i.:_?:i::::.i!?:_:i::+:::_ii:_ii_.ii::ii_!ii_!_!::_:::i_i._:::_:::_::_?:_7:.:Ui<.:0_.:0_0._1.__;._A::_:i.:%#:_:_!_i:._:
:::::::!::::.
! ::::iii:i!!;i!:i:i!_:;::ii_ 1.93 _./_.%
:_:_:_::_:_::::::_:_;_:::_::._.::: : :: "::
:::
8.41 _.A_.%
None Detected
!!ii::i_:._..i..:.:._..:.=. :_:_:_._:_:_:.:_:.... :
::::::::::::::::::::::
.-.-.
...... .............
......
:_..... :_:_::":: ._.:: :i:12:1:::i ii_-ii! !::ii::::i.!:_ii_:i:i:i(:
: ::::.:..._:.:_: ::._:._: :_::::ii_.i.i."..:__::_ ._.:.:::_. :.:-: _:_.
::_::_::::::_::::_::::::_:.::_::::_::::_:::_::::_.:.:._:.:.:-.:..:.:.=:.:<: :" :::
:: "....
i_:i_!!_!::::!::i:::i::::::::::::::::::::::i::.:i:::.::.:!:?i::.i: ii:i::.i::: : _:
::_::::i::iii::::_i::__::/::_:_:_: i:_i:_::_:::::: ::.::.::.::::i:_:.::::_:::.-:.::
0.33 _f./wt.% None Detected Not Applicable 3
1. <0.015 _.A_.% 2. 0.59 ,,./wt.% 3. <0.040_._.A_.% 4. <0.009_./v_.%
5. <0.006 _./wt.% 6. <0 007 wt./x_.% 7. 8.76 wt.A_t.%
2. PFPA
3. I-IFBA 4. NFPA Elemental Analysis6: 1. Carbon 2. Hydrogen 3. Nitrogen 4. Sulfur 5. Fluorine
::::_;:_::: :::_: "
::::::.:_i:iiii:i:::ii::j;!ii:iiii:i_:?:i:ii:;i:.i!_ii:ii:::::::i_iiii!:i!ii:i.:_:.ii:.:::_:;i: ii:::::-:;:i:_!:;:i::_:.__:: :: ; ....... ::: ._, :;_i::_i :!:::. ii; "
1. Theoretical Value = 17.8% 2. Theoretical Value = 0% 3. Theoretical Value = 0% 4. Theoretical Value = 5.95% 5. Theoretical Value = 60%
2. <0 1 _.A_.%
3. 0.10 ,,./wt.% 4. 0.28 wt.A_.%
1. 12.48 wt.A_t.% 2. 0.244 wt./wt.% 3. 1.74 wt./wt.% 4. 8.84 wt./wt.% 5. 54.1 wt./wt.%
COA023-O18A
Page 1 of 3
INTERIM CERTIFICATE OF ANAL YSIS
Centre Analytical Laboratories COA Reference #: 023-018A Date of Last Analysis: 08/31/00 Expiration Date: 08/31/01 Storage Conditions: Frozen 7M0C Re-assessment Date: 08/31/01
_Purity = 100% - (sum of metal impurities, 1.45% +LC/MS impurities, 8.41%+Inorganic Fluoride, 0.59%+NMR impurities, 1.93%+organic acid impurities, 0.38%+POAA,
0.33%) Total impurity from all tests = 13.09% Purity = 100% - 13.09% = 86.9%
2Potassium is expected in this salt form and is therefore not considered an impurity.
3purity by DSC is generally not applicable to materials of low purity. No endotherm was observed for this sample.
4Sulfur in the sample appears to be converted to SO4 and hence detected using the inorganic anion method conditions. The anion result agrees well with the sulfur determination in the elemental analysis, lending confidence to this interpretation. Based on the results, the SO4 is not considered an impurity.
5TFA
HFBA NFPA PFPA
Trifluoroacetic acid
Heptafiuorobutyric acid Nonofluoropentanoic acid Pentafluoropropanoic acid
6Theoretical value calculations based on the empirical formula, CgFITSO3"K +(MW=538)
This work was conducted under EPA Good Laboratory Practice Standards (40 CFR 160).
COA023-O18A
Page2 of 3
INTEM CERTIFICATE OF ANAL YSIS
Centre Analytical Laboratories COA Reference #: 023-018A
LC/MS Purity Profile:
Impurity C4
C5 C6
C7 Total
wt./wt. % 1.22
1.33 4.72
1.14 8.41
Note: The C4 and C6 values were calculated using the C4 and C6 standard calibration curves, respectively. The C5 value was calculated using the average response factors from the C4 and C6 standard curves. Likewise, the C7 value was calculated using the average response factors from the C6 and C8 standard curves.
i
Prepared By:
David S. Bell
Date
Scientist, Centre Analytical Laboratories
Reviewed By:
John Flaherty
Date
Laboratory Manager, Centre Analytical Laboratories
COA023-018A
Page 3 of 3
I
' ldlife
International, sLtd;
ECOTOXICOLOGY & ANALYTICAL TESTING
September 27, 2000
Ms. Susan A. Beach 3M Corporation Environmental Laboratory 935 Bush Avenue 3M Building 2-3E-09 P.O. Box 33331 St. Paul, Minnesota 55133 Dear Susan:
Enclosed please find Addendurns for Wildlife International, Ltd. Project Numbers 454A-101, 454A-102, 454A-103A, 54A-104, 454A-105,454A-106, 454A-107, 454A-108 and 454A-109
If you have any questions, please let me know.
Sincerely, .. A
KurtR.Drottar Senior Biologist
KRD/cyw8/3m Enclosures
8598 Commerce Drive Easton, Maryland 21601 Tel. 410-822-8600 Fax 410-822-0632 E-mail ecotox@wildtifeinternational.com EuropeanOffice: OverscheensewegIIA - i412AA Naarclen-TheNetherlandsTel.31(0)356949261 Fax31(0)356948229 E-mailaleopold@worldonline.nl