Document XRKyDqYKz3VeD2VJDBYm2gGV4

AR 26 _ 0724 ACUTE TOXICITY TO FISH (MUMMICHOG) TESTSUBSTANCE Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203. (1Octanesulfonic acid) (CAS# 2795-39-3). Remarks: The 3M production lot number was 1. The test sample is FC203. Current information indicates it is a mixture of 1.34% PFOS, 35% diethylene glycol butyl ether, 37.85% water, 20% ethylene glycol, 2.66 % Sultone foamer, 3% sodium octyl sulfate, 0.1% sodium lauryl sulfate, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOO: 0000 00000 Method: DTNSRDC Standard Static Marine Bioassay Procedure for Shipboard Chemicals (Liberatore). Type: Acute static GLP: No Year completed: 1979 Species: Fundulus heteroclitus Supplier: Commercial supplier in Massachusetis. Analytical monitoring: Temperature, pH, salinity, and DO Exposure period: 96-hours Statistical methods: LCs values calculated using the Stephan computer program, 1978. Testfish age: Juveniles Length and weight: Average length = 43 mm Average weight = 0.91 g (wet) Loading: Not given. Pretreatment: None Test conditions: Dilution water: Instant Ocean dissolving in deionized water Dilution water chemistry: pH 82 Salinity 15 ppt Alkalinity: 52 mg/L as CaCO; Conductivity: 23,000 umhos/cm Lighting: A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period. Stock and test solution preparation: The test solution was added directly to the dilution water and mixed by stirring with a glass rod. 000800 Concentrations dosing rate: Once SEtxapboilsiutryeofvetshseetless:t c1h9e.6miLcagllasssoljuatrisocnosn:taiNnoitngno1t5edL of test solution. Numberofreplicates: 2 Number of fish per replicate: 5 NWautmebrecrhoefmicsotnrcyendturraitnigontsh:e sfitvuedypl:us a blank control Salinity range: (0-96 hours) 15-17 ppt (control exposure) 16-17 ppt (3600 mg/L exposure)" pH range (0-96 hours): 7.28.1 6.7-8.1 ((c3o6n0t0rolmge/xLpoesxuproes)ure) Temperature range (0-96 hours): 19.5 C Dissol6v.e2d-o8x.y3gmegnlLra(ncgonetr(o0l-9e6xphoosuurrse)): - 30-82 mg (3600 mg/L exposure) RESULTS Nominal concentrations: Bk control, 600, 1000, 1700, 2400, and 3600 mg/L Element values: 96-hour LCS0 = 2500 (1700 -- 3600) mg/L. 96-hour NOEC = <600 mg/L. Element values based on nominal concentrations Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. coNCLUgiONS The test sample 96-hour LCso for mummichog was determined to be 2500 mg/L with a 95% confidence interval of 1700 to 3600 mg/L. - Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 p0 ata0 Quau0 ry 0000000000 RHeolwiaebvileirt,y:thKelismaimspclherapnukriitnygw=as2. nToetsptrionpgermleyecthsartahcetcerriitzereida afonrdqtuhaleitsytutdeysting. lacks analytical confirmation of the amountoffluorochemical proportion in the solution. REFERENCES 000601 Test was conducted by EG&G Bionomics, of Wareham, MA at the request of the 3M Company, St. Paul, MN, Lab Request number 4971S, 1979. o0 rHER 000000 Last changed: 6/27/00 000602 3 ACUTE TOXICITY OF 7902 TO THE COMMON MUMMICHOG (Fundulus heteroclitus). Fe-203 fot] LR 497s EAT yas 79245 TOXICITY TEST REPORT SUBMITTED TO : THE 34 COMPANY ) ST. PAUL, MINNESOTA REPORT #BW-79-8-526 AquatiEcGT&oGxi,colBioognyomLiacbsoratory Wareh7a9m0, MMaianssaScthruesetetts August, 1979 . 000603 . Fo 23 ) nwmooverzo The purpose of this study was to estimate the acute toxicity of 7902 to the common mummichog (Fundulus heteroclitus) under static conditions. A 96-hour definitive test was conducted from 27-31 July 1979 at the Aquatic Toxicology Laboratory of E G &G, Bionomics, Wareham, Massachusetts. stored at the above location. All raw data generated are - MATERIALS AND METHODS Unless otherwise stated, procedures used in this acute toxicity ) test followed those described in "DTNSRDC Standard Static Marine Bioassay Procedure for Shipboard Chemicals" (Liberatore). All values are reported to various levels of significance v depending on the accuracy of the measuring devices employed during any one process. The 7902, an amber liquid, received from the 34 Company, St. - Paul, Minnesota on 6 July 1979, was tested on a formulation basis. Nominal test concentrations are reported as milligrams' of 7902 per liter of test solution (mg/%). The common mummichog (lot #79A7) used in this study were ob- tained from a commercial fish supplier in Massachusetts. Upon J receipt at Bionomics, the fish were held in a 600-2 fiberglass 000604 2 N tank containing a solution of Instant Ocean which was recircul- ated through dolomite in a biologically active filter bed to preserve water quality during holding and acclimation. This water was characterized as having a pi rangeof @o1.2) a aissolyed oxygen (DO) range of 89-30% of saturation (Weekly Record of Fish Holding Water Characteristics, Vol. I), a specific conductance range of 18,000-23,000 micromhos per centimeter (umhos/cm), a salinity of 15-19 parts per thousand (0/00) and a temperature | range of 19.0-20.5C (Daily Record of Fish Holding Conditions, July 1979). Experimental aninals were maintained under these I conditions for a minimum of 14 days. All fish were fed a dry i ) pelleted food; ad libitum, daily except during the 48-hours prior | to testing. There was no mortality during this 2 day period. i The pH was measured with a Model #175 Instrumentation Laboratory | "pH meter and combination electrode, the temperature with a dial [ thermometer, the DQ with a Model $57 YSI meter and probe, the | specific conductance with a Model $33 YSI conductivity meter, / and: the salinity with an American Optical refractometer. / The definitive test was conducted in 19.6-1 glass jars which + / _-sentsined 15.1 of tess solusion. The dilution vater used fn / this test was prepared by adding Instant Ocean to deionized water and mixing until dissolved. - This water had a pif (53) a total alkalinity as calcium carbonate of 52 mg/% (APHA et al., / 1975), a specific conductivity of 23,000 umhos/cm and a salinity 000605 3 \ of 15 0/00 (Reconstituted Water, Water Quality Analysis, Vol. II). The 7902 was added directly to the dilution water and mixed by stirring with a glass rod. re Control jars (A and B replicates) containing the same dilution water and maintained under the same conditions as test concen : trations, but containing no 7902, were established. All test solution temperatures were controlled by a system designed to maintain temperatures at 19 + 1C. All test solutions were gently aerated during the exposure period. The photoperiod ) uring acclimation and testing was 16 hours light and 8 hours ) darkness. Tencommon mummichog (five per replicate) with a mean (range, . " N=30) wet weight and total length of 0.91 (0.28-2.79) grams and 43 (32-60) millimeters (Fish Weights and Lengths Log, Vol. IIT) were randomly distributed to each test concentration within 15 minutes after the solutions were prepared. : . | Mortalities vere recorded and removed from each test jar every * 24 hours during testing. Biological observations of the fish and observations of the physical characteristics of the test solutions were made and recorded at 0, 24, 48, 72 and 96 hours. The pl, DO concentration and salinity of test solutions were : measured at 0, 24, 48, 72 and 96 hours in both replicates of the control, high, middle and low test concentrations. Temperature 000606 5 4 was also measured in each control jar replicate at the above ph mentioned time intervals. | > When results permit, the concentrations tested and the corres-: ponding mortality data derived from the toxicity test are used to estimate 24, 48-, 72- and 96-hour median lethal concentrations(C50) and 95% confidence intervals. The LCS0 is defined as the concentration (nominal or measured) of the test compound in dilution water which caused 50% mortality in the test animal population at the stated exposure interval. ) The computer program utilized (Stephan, 1978, personal communi cation) estimates LCS50 values using one of three statistical . methods in the following order of preference: Zomvoviinngg aavveerraaggee . _an--gle analysis, probit analysis, binomiA al probability. The ; method selected is determined by the characteristics of the data base (i.e. presence or absence of 100% mortality, number of partial mortalities, etc.). The computer program scans the - ata base, identifies the most preferred statistical method and performs the analysis. . RESULTS The 96-hour LCSO for the common mummichog exposed to 7902, estinated by the binomial probability method, was|2,500 mg/L.| Table 1 000607 5 yo. summarizes the 24-, 48-, 72- and 96-hour LCS50's and 95% confidence intervals and states the no discernible effect concen- : tration through 96 hours. The no effect concentration is the : highest concentration tested at which there were no mortalities or observed behavioral and physical abnormalities, e.g. 108s of - equilibrium, at surface, darkened pigmentation. E The pH, DO and salinity measured during the test are presented in Table 2. The temperature of the control solution was 19.5C during exposure. ) Table 3 presents the nominal test concentrations, the corresponding cumulative mortalities and the observations made. Deviations from the Protocol Cited ; ~ The common mummichog were 4.3 centimeters long. - Nineteen point six liter widemouth glass bottles were used as test containers. - Instant OceanTM solution was used for acclimation and testing.' . = The fish were acclimated in recirculating water. - The salinity for acclimation and testing was 15 o/oo. ~ Fish were acclimated in water with a pH range of 7.0-7.2 and tested in water having a pH of 8.2. 000608 6 y - The test jars contained 15 % of solution and 5 test fish. ~- The test concentrations were replicated. i 2 - LC50's were calculated by Stephan, 1978, personal communi- 2 cation. TTC TE bo "A it is our judgment thatnoneof these deviations have affected. the integrity of the test results. - ) . . 000609 LITERATURE CITED APHA, AWWA, WECF. 1975. Standard methods for the examination of water and wastewater. 14th Edition, Washington, D.C. 1193 pp. - : Liberatore, Giacomo. David W. Taylor Navel Ship Research and Development Center. Standard Static Marine Bioassay Procedure for Shipboard Chemicals. Report TH-28-76-29. Stephan, Charles. 1978. U.S. EPA, Environmental Research Laboratory, Duluth, Minnesota. Personal communication. hb) 0C0610 y SUBMITTED BY: PREPARED BY: : Sh AquatEicG T&oxG,icoBiloongoymiLcasboratory Wareh7a9m0, MaMianssaScthrueseettts 2 August, 1979 . charlotte Stiefel Principal Investigator REVIEWED BY: Robert J. Buccafusco ) * ve i. / A 4 Study Directo: . APPROVED BY: George A. Cary ' i (= ge = birector, -- Biology DATA AUDITED BY: Ronni Krasny fluSu | Director, Quality Assrance Unit 000611 Table 1 -- The 24-, 48=, 72- and 96-hour LCS0 values and 95% confidence intervals for the common mumichog (Fundulus heteroclitus) exposed to 7902. 24 hour" >3600 1650 (ma/t) 48 hour' 72 hour' (242000-030600) (2420900-03600) 96 hour' (1275000-03600) etrentSonceneation through 96 hours (mg/t) <600? `Eeatpiimcaatleldy estimated. by the binomial probability method. . ' a`5L5o4wescto.nfciodnecnecnetraitnitoenrvatless.ted. 2828 . 2 . 4: i ii Table 2. mThaopmHs20(0unndduslaulsinihtoyrcmcelsistuerne)p.eneRmaandgeesdoufcimnegsuthreem9e6n-thsovmssdSeonItnetseyehSiecnattewisthon7ce0n7tesantdiosnhearceomon soot, ies conMsoemmihnanaizion+11 1. ` 0 hour 20 voir ina8 vue fEre 56 our | Ld e217o00 cantzworw EY BE wo SUL amy fotrony see wo 1,700 control a `ea soees 360 B[OEDsIE TM 115-16. wo 8.0-8.1 n7o.t0e0s0 reams LATTIION 2 1s15-16 1 6c.s7 era neal6e7ns a rans aris i sac A sisi i 7C1o0.r0a06m9 6s2aas(s6rsen P1M6 2 s7w mo c6r.e7 e ereinasn SChede| 4Searsasteren M16 A of saturation at 19%. ogg252 . +: ; i "able 3 -- Concentrations tested and corresponding cumlative mortalities of the common mumichog (Pundulus heteroclitus) exposed to 7902 for 24; 48, 72 and 96 va. hours. Ten fish vere exposed to each concentration, : five fish per replicate.. - : * conNcoemnitnraa1tion (ng/2) ; _Cumilautrive mortaliotuires(Aand3ourr eplicates)oor 3,600 2.400 ) 1,700 1,000 600 control obreidie yg 10 bed ba o o 0 0 0 o 0 o o o 0 0 10 bid ood o o . oo . aFoam : present on surface of all solutions, except the controls, throughout testing. PSone fish were at the surface. "some Fish had partially lost their equilibrium. . %Sone ish were lethargic. some fish vere respiring rapidly. ) 000614