Document XRBQ87nv73Y2bbGxEjpEQg8jJ

DownloadViewSend us a messageRandom document
M S M 6 _ O E S Zs MICROBIOS MICROTOX TOXICITY TEST TEST SUBSTANCE____________________________________________ Identity: A mixture containing perfluorooctanesulfonate; may also be referred to as PFOS, FC-95, or as a component of FC-206CF or FC-206CF-X. (1-Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was 101. The test sample is FC206CF. Current information indicates it is a mixture of 0.5% PFOS, 10% diethylene glycol monobutyl ether, 78.08% water, 1.375% acrylic foamer, 1.5% sodium octyl sulfate, 1% sulfonated alkyl ether salts, 6.0% urea, 0.5% triethanolamine, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect the toxicity of the fluorochemical component of the test sample. METHOD:____________________________________________________ Method: Microbios Microtox "BASIC" Procedure Test type: Acute static GLP: No Year Completed: 1991 Species: Photobacterium phosphoreum Analytical monitoring: pH and osmotic condition. Exposure period: 30-minutes Statistical methods: EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbios' Reagent. Test conditions: Diluent: Millipore Milli-QTM water Test temperature: Not noted. Stock and test solution preparation: A primary 2000 mg/L stock solution was prepared by dissolving 200 mg of test substance in 100 mL of Millipore Milli-QTM water. The pH of the stock solution was 6.5, and an osmotic adjustment was made using 200 mg NaCI dissolved in 10 mL stock solution. Appearance of test solutions was noted as "clear, colorless". All test solutions were made by proportional dilutions. Exposure vessels: 4 mL cuvettes. Number of replicates: two Number of concentrations: Four plus a blank control Element basis: Percent light loss R ES UL TS. Nominal concentrations: Blank control, 125, 250, 500, and 1000 mg/L Element value: 5-minute EC5o = >1000 mg/L 15-minute EC50 = 890 (730 - 1000) mg/L 30-minute EC5o = 680 (6 1 0 -7 5 0 ) mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ The test substance 30-minute EC50 for Photobacterium phosphoreum was determined to be 680 mg/L with a 95% confidence interval of 610 to 750 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133 DATA QUALITY_______________________________________________ Reliability: Klimisch ranking = 2. The study meets all criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. R E F E R E N C E S _____________________________________________ This study was conducted by the 3M Environmental Laboratory, St. Paul, MN, Lab Request number J1884-4,1991. OTHER______________________________________________________ Last changed: 6/28/00. 001894 A ENVIRONM ENTAL LABORATORY M ICROBIOS M ICRO TO X SYSTEM RAW DATA jLR X / g g f r pq_L o f jH T E S T S U B S TA N C E : F t - 2 Q(p C FT E S T D A TE: 7/11 h i _______ /Ol BEST COPY AVAILABLE ANALYST: X. A oaJ L SAMPLE CUVETTE r*A)L FINAL"ASSAY CONCENTRATION INITIAL REAONG 1(0) FINALREADINCiS 5-MIN 15-MIN 30-MIN 1151 _ I (15) 1(30) ,yv*'. - Bl BLANKCONTROL L>0 47 4M CM DUPLICATE 0 7 73 S? t 8 ty-` *''->V . ; gs /25* I>uplica.K. 75o O 1 17 \^ 70 5i 5* duplcate.'/ fl. 5H va 37 i 1) <?& '-iA^ins. ->'- *!< ' * m fi h w & f 5 - .'vv`-'.r-ji v?" ; 2>vp)Ya.+- *- 1 . 'T ; ' li lo i . r. -f. S ilk h y ijin ^ r 5 & - ; . 31 3 S e;- 3.2 im M I 33 3o >?<=>, ^i ;\Vy<* -A*' X-U& 1 --i r'*3' ' nc of tost."solution^ Ufilu ~; 001895 'iW'i'r' - te - ' BEST COPY AVAILABLE {*,\ J} ?; '>p.>*- .. ; j.' t MICROTOX(r) DATA SHEET V \>i F C - 2 0 6 C F Lo t 101 5-min sxo. J1 88 4- 4 7/19/91 *ab !='Sl' . ' - PAIR # CONC. Io/It 125.000 125.000 250.000 250.000 500.000 500.000 XI000.000 XI000.000 110. 0/ 68.0 117.0/ 70.0 112.0/ 64.0 98.0/ 54.0 110.0/ 52.0 104.0/ 48.0 104.0/ 38.0 110.0/ 39.0 BLANK Bo/Bt= 100 / 67 BLANK RAT 10= 0.6700 S-OBS 0.084 0 . 120 0.173 0.216 0.417 0.452 0.834 0.890 EC 50 = X I 120.493 < 923.602 TO XI359.357 > Cf0 > l J 1' R 0.99039 SLOPE = 0.9361 INTERCEPT +7.0215 B-EST *. - l osSYO 0.098 0.098 0.203 0.203 0.419 0.419 0.867 0.867 <2_-PV^ X / O d L 146-ofeO 0 +----------------- 0--------- - - - - V------------------. # # RES. PROB. ! ,, -- 8 - . ' 1 0.195 100 !> r - ---- 7-. .2 0.158 , 66 I >' ----- -- . pi 127.' - 43 ! - -------- ;-:v`. y 2//' . 0.-14...2' 34 : ! v- ---- L -6--- , , ,, v*. * o ^ ^ O i l 2 1 % , ': .;:f39,, "1 > ^ ' --- 5" <` G 0,174" V 80 v , - 6.. './v. , **" ' *.--- 7 ; ' i *6.:- K H -J t - v 'v, ; JMlMMmt^lte _____<. . _ .. i w S Lj*' vJRb<3JLt e r e d r o bLjfSSPPPCATLoc *1* t r;`(x')fl 001896 .-A. U: Vf R.' ' F C -206C F L o t 101 AVAILABLE -7. A?' V ' MICROTQX( r ) DATA SHEET 13-m in e x o . J 1884- 4 7 /9 /9 1 ab PAIR * ' CONC. lo /It 1 -2 3 .-4 5 6 .7 8 12 5 .0 0 0 12 5 .0 0 0 2 5 0 .0 0 0 2 5 0 .0 0 0 5 0 0 .0 0 0 5 0 0 .0 0 0 7 .1 0 0 0 .0 0 0 7.1 000 .0 00 1 1 0 .0 / 5 2 .0 1 1 7 .0 / 5 6 .0 1 1 2 .0 / 5 1 .0 9 8 .0 / 4 2 .0 1 1 0 .0 / 3 9 .0 1 0 4 .0 / 3 5 .0 1 0 4 .0 / 2 6 .0 1 1 0 .0 / 2 7 .0 BLANK B o / B t * 1 0 0 / 5 3 . BLANK R A T IO * 0 .5 3 0 0 G-OBS 0.1 21 0 .1 0 7 0 .1 6 4 0 .2 3 7 0 .4 9 5 0 .5 7 5 1 .1 2 0 1.1 59 EC 5 0 * 8 8 5 .9 9 7 : < 7 3 3 .3 4 8 T0 7.1070.421 > C$o~ &<io ( 7 3 0 - / 000) ^ ^ / ^ R *0 .9 8 7 7 0 SLOPE = * 0 .8 5 5 8 INTERCEPT = + 6 .7 8 6 7 j i w -H 8 -E S T 0 .1 0 4 0 .1 0 4 0 .2 3 0 0 .2 3 0 0 .5 0 7 0 .5 0 7 1.1 1 8 1.1 1 8 %> il *7 n (4 33 37 54 <0, 3 -o b s ,, ( I *G~bs3 /y V- '% > y lo 17 3 f SV 0 + ---------- :--------------------- V-- ------------------------- V---------------------------. 8 - + ## RES. .1 ' 0 .1 8 5 ;: i '. ' --------- . ! .,2 . 0 . 1 9 8 - --------- . 'I ' .V .< 3;V! 0 .1 5 7 , ; 0.131 > f t/ r / -',41 ; ' . Ii ,:i * Ih* 1 .7 -, " 0 . -201` ft'--96,r r-> ' i - ' V:' ;- "- ` -, .. ;; -----56---,,------- -------- * ------ ^ i *! * :,.t . y I w ,, 8 - .0.205 i' & \ ' --JC W 4>>i.-VJV - >; -,'--r.-Sr'j*A-- jS'gyvy "'-3-j. fe vi'*v`.JT SOTO ' i1'*' ra r' X ..ir , Hr ,5' Pfc / / \'U ;tw iV ' 'V. ^ V ` : - .,\ . MI CR OT OX <r ) D A T A ,SHEET, , V F C - 206C F L o t 101 30 -min xo. J 1884- 4 7 / 19/91 sab '.+ v - < '^ . < . ; P A I R ' ^ v C O N,C* .\^ry- 1 2 '3 4 5 6 :,' 7 .8 125.000 125.000 250.000 250.000 500.000 500.000 % 1000.ooo 7.1000.0 0 0 ; : l O / It 110.0 / 48.0 117.0 / 52.0 112.0 / 45.0 9 8 .0 / 37.0 110.0 / 33.0 104.0 / 30.0 104.0 / 20.0 110.0 / 21.0 G-OBS 0.146 0.125 0.244 0.324 , 0.667 0.733 1.600 1.619 . BLANK Bo/Bt= 100 / 50 BLANK RATIO" 0.5000 EC 50 - 676.026 ( 612.149 TO 746.568 ) < ~5 > = 2>o 11, id - R =0.99517 SLOPE = 0.8227 INTERCEPT = / 1- +6.5162 8-EST -^h+ \ 0.130 0.130 0.300 0.300 0.690 0.690 1.590 1.590 I i) ao H ho ha. yx ux V \' C,)- ^ bS X /CO ^ / 6-cbs) X ix Lx R E S . PROB. 0.119 ;83 0.129 QO . 0.091 -V,'-, 50,v-vi. 0.081 0.082 0.085 0 -127 0 0.128 ^ ,,' M v5\.V,V' VtAa:,'.'-- U*W tt wm r v%On"'.O, `:; f e % [ M |fe: R--. -"t--57-+r*^d>TSre&sa&sXM^-al i!* f1-' `^yt* /< 001898
Contact UsLoginSign Up
CUNY - The Graduate CenterColumbia University Mailman School of Public Health - Sociomedical Sciences