Document XOj7BZ5eo3Q7LmVN0bG5pbkKy

A f t 2 6 - 01&2> TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE________________________________________________ Identity: Mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203CF-X or FC-203CF. (1-Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was 101. The test sample is FC203CF. Current information indicates it is a mixture of 1% PFOS, 20% diethylene glycol monobutyl ether, 70.2% water, 2.75% Acrylic foamer, 3% sodium octyl sulfate, 1% triethanolamine, 2% sulfonated alkyl ether salts, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD:______________________________________________________ __ Method: OECD Guideline 201 Type: Acute Static GLP: No Year study performed: 1991 Species: Selenastrum capricomutum Source: In-house culture. Originally from the Culture Collection of Algae at the University of Texas at Austin, Texas. Element basis: Algal cell counts (cells/ml), growth rate Exposure period: 96 hours Statistical Methods: Statistical analyses were performed using the computer program of Stephan, 1983. Analytical monitoring: pH, conductivity, and temperature Test Conditions: Algal Nutrient Medium: Nutrient medium per U.S. EPA, 1978. This nutrient medium provided all mineral nutrients essential for algal growth and also served as the diluent for all algal operations. The pH of this synthetic algal medium was 8.0. Stock and test solution preparation: A primary 1000 mg/L stock solution was prepared in algal medium, and added directly to the exposure vessels. _ Exposure vessels: 250 mL Erlenmeyer flasks containing 100 mL test solution and capped with inverted glass beakers. Agitation: Continuous Number of replicates: 3. Initial cell loading: 1.0 x 104 ceils/mL. Number of concentrations: five plus a blank control wnzss Lighting: Continuous lighting at 44 pEin/sec/m2 using cool-white fluorescent lamps. W ater Chemistry: pH range (0 - 96 hours): 8.0 - 10.8 (control exposure) 8.2 - 8.3 (1000 mg/L exposure) Test temperature range (0 - 96 hours: 21.9 - 22.3C (incubator) RESULTS_________________________________________________________ Nominal concentrations: Blank control, 62.5,125, 250, 500, and 1000 mg/L 96-Hr. Algal Growth Response values Nominal Exposure Cone. (mg/L) Control 62.5 125 250 500 1000 Cell-Count (cells/mL) 602,000 451,000 207,000 12,000 0 0 Avg. Specific Growth rate 0.043 0.040 0.032 0.002 0.000 0.000 % Change from Control -- 7 23 95 >100 >100 Element values: 96-hour ErC5o = 143 (130 - 156) mg/L 96-hour NOEC = 62.5 mg/L Element values are based on nominal concentrations. Reversibility of Growth Inhibition: Not conducted. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS___________________________________________________ The test substance exhibits a 96-hour ErCso of 143 mg/L with a 95% confidence interval of 130 to 156 mg/L. The 96-hour No Observed Effect Concentration (NOEC) was 62.5 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY____________________________________________________ 001259 Reliability: Klimisch ranking = 2. The study meets the criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES This test was conducted by EnviroSystems Division, Resource Analysts, Incorporated, of Hampton, NH at the request of the 3M Company, St. Paul, MN, Lab Request number J1884-3,1991. OTHER_____________________________________ :_____________________ Last changed: 6/28/00 001260 BEST COPY AVAILABLE Z. SOMKART The cut* toxicity of rc-203CT to the alga, Salanaatrua capricomutua, i described in this final raport. The teat was conducted for 3M Company for 96 boura during July 15 to 19, 1991, at tha BnviroSystems Diviaion of Resource Analysta, Inc. In Hampton, Haw Hampshire. It waa conducted by Joanna Magazu, Patar Kowalski, Bilan Stanford, Robert Boori, and Timothy Hard. Tha tost waa performed under atatic conditions with five concentrations of teat substance and a dilution water control at a mean temperatura of 22.1*C. The dilution water waa synthetic madia prepared with atarila deionised water. :Blaska ware Incubated on a rotary shaker. Light was adjuntad to 44 uSin/aec/m2 with a photoperiod of 24 hours light and 0 hours dark. Nominal concentrations of FC-203CF tara: 0 tng/L (control), 62.5 mg/L, 125 mg/L, 250 mg/L, 500 mg/L, and 1,000 og/L. Nominal concentrations ware used for all calculations. Algas uaad in tha test waa from an in-house cultura that waa maintained under teat conditions. Bxposure of algae to tha teat substance resulted in a 72 hour BC50 of 130 mg/L FC-203CT and a no observed effect concentration (NOBC) of 62.5 mg/L. The 96 hour BC50 and HOBC are 143 mg/L and 62.5 mg/L, respectively. ii. t a bl * or c a n o n s SECTIONI I. Suaoary II. Tabl* of Content H I . Indax of Tabl** IV. Introduction , V. Kethoda and Material* VI. Results \ 'r-.*.' v.. .- -..7 VII. References - <: Appendix A. - Hatar Quality Data from Toxicity Tost tJEST COPY AVAILABLE III. INDEX or TABUS Table 1. Chemical characterization of a representative sample of deionized water used to prepare test media for toxicity test Table 2. Growth data from toxicity test Table 3. Percent change from control and average specific growth rate from toxicity teat Table 4. Median effective concentrations (BC50s) from toxicity test Table A.l. Temperature measured during toxicity test Table A.2. pa measured during toxicity test PACE 6 9 10 11 14 15 BEST COPY AVAILABLE T*t>l* X. Chemical characterisation of a representative sample of daionizad water used to prepare tast nadia for toxicity test Parameter Unit of Measurement Reporting Limit Meaaured Value Organochiorine peaticidee ug/L 2 NO Organophosphorua peaticidea ug/L 0.5 " ND Polychlorinated ug/L 0.5 ND biphenyla Note: ND " not detected at or above the reporting limit. BEST COPY AVAILABLE The test was conducted at a target temperature of 22 t 2*C with five concentratlone of test substance and a dilution water control. A 1,000 mg/L stock solution was prepared in dilution water. The stock solution was added directly to dilution water contained in the test vessels without the use of a solvent. Homlnal concentrations of the test material weret 0 mg/L (control), 62.5 mg/L, 125 mg/L, 250 mg/L, 500 ag/L, and 1,000 mg/L. Algae was randomly and equally distributed among three replicates of each treatment at the rate of 10,000 cells/ml. The test was performed in 250 ml glass Erlenmeyer flasks that contained 100 ml of test solution. The flasks were capped with inverted glasB beakers. Test vessels were randomly arranged on a rotary shaker in an incubator during the 96 hour test (a random numbers table was used to select the location of each vessel). A 24 hour light and 0 hour dark photoperiod was maintained with cool-white fluorescent lights that provided a light intensity of 44 uSs-1m-2. The number of algal cells/ml in each test vessel was determined visually every 24 hours by means of direct microscopic counts with a hemacytometer. The pH (Beckman model pHI 51 meter) was determined in each test flask at the beginning and end of the test, and temperature (ASTM mercury thermometer) was measured and recorded daily in the incubator. STATISTICAL METHODS t Results of the toxicity test were interpreted by standard statistical techniques (Stephan, 19S3). The binomial or moving average method was used by the author to calculate EC50 values using nominal ... J-Dunnett's test, which includes a parametric one-way analysis of *",^variance (AHOVA) The average specifie.`growth rate was calculated as the ITnatural log of the ntmdjer ijOfJ^lle^per ml at 24, 48, 72, or 96 \ ^ hours minus the natural `.log,of. themimherof cells per ml at 0 hours - vV 'V divided by the exposure. period^||The^"percent change from control'is 38'* calculatedJi by subtracting Uie|s<^r^avwage specific growth rate'jfram^ .the^control average specifin^growth irate, `dividing 'that; value by.the average ;specific growth'^;rate|in;th^^Mtroi'Jand multiplying that Value' by 100. The percent change!from controlCvaa used to compute EC50 and M WOECtvalues.:, . as1!.< '7,v>' * v* Kf > M ', < - & ,,. COPY AVAILABLE Biological and watar quality data generated by the acuta toxicity teat ara presented in Table 2 and Appendix A, respectively, and the percent of control and average specific growth rate information ia presented in Table 3. The 24, 48, 72, and 96 hour BCSOs for algae exposed to PC-203CF are presented in Table 4. The 72 hour EC50 ia 130 mg/L, and the 72 hour NOEC ia 62.5 mg/L PC-203CP. The 96 hour BC50 ia 143 mg/L and the 96 hour NOEC ia 62.5 mg/L. 125 1 10 20 44 52 220 2 10 18 80 58 198 3 10 22 58 74 202 mean 10 20 61 61 207 250 1 10 16 14 12 16 2 10 22 20 12 12 3 10 18 20 10 8 mean 10 19 18 11 12 500 1 10 12 6 4 0 Tabi* 3. Percent change fron control and average specific growth rat* from toxicity t*at Nominal ; concentration of Test Substance (mg/1) Average spec'lie growth rate 24hr 48hr 72hr 96hr Percent change from control 24hr 48hr 72hr 96hr 0.0 (control) . 62.5 125 250 500 1,000 0.048 0.053 0.046 0.043 0.031 0.040 0.040 0.040 0.029 0.038 0.025 0.032 0.027 0.012 0.001 0.002 0.020 -0.002 -0.016 0.000 0.000 -0.009 -0.009 0.000 0000 35 24 13 7 40 28 46 23 "44 77 98 95 58 >100 >100 >100 100 >100 >100 >100 BEST COPY AVAILABLE Table 4. Median effective concentrations (SCSOa) from toxicity taat Exposure period BC50 95 percent confidence limits Calculation method 24 houra 48 houra 72 houra 96 houra 212 mg/L 156 mg/L 130 mg/L 143 mg/L 182 - 244 ag/L Moving Average 140 - 174 mg/L Moving Average 120 - 141 mg/L - Moving Average 130 - 156 mg/L Moving Average tkiv &*% BEST c o p y a v a il a b l e -,v & -v^i* m .**,*, iff rT, Ar. 5 i V1<.,*# i8**tsfti :T.,Z'S*mestem.emss>^mi 001271 /X vii. bsteren cbs OECD. 1984. Guidelines for Mating of Chemicals. Section 2t Effect on Biotic Systems. Method 201, M,a, Growth Inhibition Teat. Adopted June 4, 1984. Stephen, C.B. 1983. Computer program for calculation of LC50 values. Personal communication. O.S. EPA. 1978. The Selenastrum capricomuCua Prints Algal Assay Bottle Test. BPA-600/9-78-018. July, 1978. BEST COPY AVAILABLE 'i' 'J r/>. -:i X ' . \.-hy V" Ww *hI i ."io* I *0IAUPOttE ftfS 001272, LABLE ftPW n<UK ftt----W AH R QUALITY DATA FROH T O X IC IT Y T K ST