Document XOX1gz4ojqMZedG534mVjaVYw
TOXICITY TO AQUATIC PLANTS
TEST SUBSTANCE
Identity:A mixturecontaininpgerfluorooctanesulfonawthei,ch may alsobe referretdoas PFOS, FC-95,or as a component of FC-603EF. (1Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productiolnotnumber isnotnoted.The testsample is FC-603EF. Currentinformatioinndicateistisa mixtureof 1.30% PFOS, 10% diethyleneglycolbutylether,54.15% water,22.5% ethyleneglycol2,.6% propanesultonefoamer,5% Alkylethesrulfate0,.1% N-(3Chloroallyl)hexaminicuhmloride0,.9% Xanthan gum, 0.9% starch,1.0% triethanolaminaen,d 0.05% tolytlriazole.
The followingsummary appliesa mixture withincompletely characterizedconcentrationsof impurities.Data may not accurately reflecttoxicityof the fluorochemicalcomponent of the testsample.
METHOD:
Method: OECD Guideline201 Type: Acute static GLP: No Year completed: 1996 Species: Selenastrumcapricomutum Source: In-housecultureO.riginalflryomATCC, RockvilleM,D Element basis:Algalcellcounts(cells/mLa)nd averagespecifigcrowth rates. Exposure period: 96 hours StatisticaMlethods: IC50 valuesdetermined by the Trimmed SpearmanKarber Method and Toxstatsoftwarepackage,Version3.5. Analyticalmonitoring: pH and temperature.
Test Conditions:
AlgalNutrientMedium: Per OECD method. Thisnutrienmtedium provided allmineralnutrientesssentiafloralgalgrowth and alsoserved as thediluent forallalgaloperations.The pH ofthissynthetiaclgalmedium was 9.06.
Stock and testsolutionpreparation:A 1000 mg/L primarystocksolution was preparedby dilutin1g50 mg oftestsubstancein150 mL ofstandard algalnutrienmtedium. Aliquotswere thenadded directltyodilutiownater in testvesselsto createtestsolutions.
Exposure vessels: 125 mL borosilicate-glEarslsenmeyerflaskscontaining 50 mL oftestsolutionand capped withfoam stoppers.
AgitationS:hakencontinuousalty100 rpm. Number ofreplicate3s:forexposureconcentratio6nfso,rblankcontrol. Initicaelllloading:1.0 X 104 cells/mL(1.2x 104 actual) Number of concentrations:fiveplusa blankcontrol Lighting:Continuous400 ft-candieussingcool-whitfeluorescenltamps.
Water Chemistry:
pH range (0-96hours): 7.48- 9.06 Test temperaturerange (0-96hours): 24.2- 25.8'C
RESULTS
Nominal concentrations: Blank control,31.25,62.5,125,250, and 500 mg/L
Algal Growth Response EC50 values
Exposure (Contact)
Hours 24 48 72 96
Cell-Count mg/L (95% C.I.) 139 (117- 165) 153 (124- 189) 188 (173-204) 141 (123- 162)
Average Specific Growth Rate
mg/L (95% C.I.) 171 (146- 199) 290 (254-330) 275 (252 -300) 294 (265 -326)
Element values are based on nominal concentrations.
Reversibilityof Growth InhibitionA:liquotsofthe 500 mg/L testsolution were dilutedwithalgalmedium and culturedfor7 days. Based on growth observed inthe recoveryphase,the effecton algalgrowth was found to be algistatic.
Remarks: Testingwas conducted on the mixtureas described inthe Test Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The testsubstance exhibitsa 96-hourEC50 cellcount value of 141 (123 - 162 mg/L) and a 96-hourErCrogrowth ratevalueof294 (265 - 326) mg/L.
The 96-hourNo Observed EffectConcentration(NOEC) is31.25 mg/L forcell count and 125 mg/L forgrowth rate.Thistestsubstance was determined to be algistatic.
Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133
DATA QUALITY
ReliabilityK:limischranking= 2. Thisstudymeets thecriterifaorquality testing.However, sample puritwyas notpropedycharacterizeadnd thestudy lacksanalyticaclonfirmatioonftheamount offluorochemicaplroportioninthe solution.
REFERENCES
Thisstudywas conductedby AScl CorporationA,Scl-DuluthEnvironmental TestingDivisionD,uluth,MN attherequestofthe3M Company, Lab Request number P2581, 1996.
OTHER
Lastchanged: 6/26/00.
AS,:[C,,rl%,rAts.,ii,AS,:[-Duluil@ I'n%iriiiiiiTi%c:i.i@ttiiDtnig%isititt
AS,,:lStud.vIDJR 5010-Obg
STUDY TITLE GROWTH INHIBITION OF FC-603EF. LIGHT
ATC 3%, AR-AFFF TO GREEN ALGA (Seletiastrciaipir?it-oriiiiiiiiii)
DATA STANDARI) 01--CD GUIDELINE 201
STUDY CO,%IPI.EI'Et) Noveiiiber22. 19k)O
TESTING FACILITN' AScl Corporation,ASc-iDuluth EnvironmentalTestinC,gDivision
4444 Airpark Boulevard Duluth,NIN 55811
Tel.No. (218)722-4040 F ax N o. (1.18) 7'@)-.')5-9)2
STUDY IDENTIFICATION NUMBERS AScl Study ID# 5010-068
3M Company Lab Request# P2581
S;)onsor. 3M Company
SponsoSrtudyIDIP".581
AS,.l Efl,ir,wnitici,.TtLa:t,EitilD:t%i.i,,n
AS,:l St@,dl@ll)g 5010 068
1.0 GENERAL INFORNIATION
Study Title
Data Standard Sponsor
Sponsor's Representative
Sponsor's Lab Request# TestingFacility
PrincipalInvestigator ProjectDirector TestingFacilityDirector DefinitiveTest Dates Data Validationand Report Review
Growth Inhibitioonf FC-603EF. l.iahtWater (TM) ATC 3%, AR-AI:Fl:to GreenL AIL--A (Selenastriicta?piriet)riiiiiiiiii).
OECD Guideline201.
3NI Environmental Techiltolt),a,i,l@dSer,,,ices. Building2-3E-09, 935 BusliA%,cntie.St. I'aLli. MN 55133, Tel No. (612)778-7452.
Susan Beach. 3NI En%-ironniciiial and Services,Buildine"-3E-09. 93i liLISI) Avenue. St. Paul. NIN i@,133: Tel No. (612)778-7452.
P258 1.
AScl Corporition,@ASci-I)LIl1L-I1t)iI'lrt)[Illlelltil TestingDivision.4444 Airl).trBkotile%iirki. Duluth,MN 558i 1: Tel. No, ("18) 722-4040.
Todd NlcGuire
Joe Amato
Donald Mount
Novei-nber4-8, 1996.
Alan Niozol
Report Si,,iiattire:
Retentionof Raw Data and Final Report Location of Raw Data and FinalReport
Two years from thedate study iscompleted.
ASci Corporatioti/ASci-DtiltEintvhironmental Testinc,Division,4444 Airpirk Boulevard. Dulutoh,MN 55811; Tel. No. (218)722-4040.
Sponsor; 3M Company
SponsoSrtudyID# r-581
2
@%S. I Si...l@ ll)z 50!0 L)68
2.0OBJECTIN'E(S)
To determinethe4-d medianeffecctoncentrati(oEnC,,,t)h.e4-dno obser,ableeffect concentrati(oNnOEC), andalgicidal/asltgaitiecffecotf thetestsubstiiii1c:e(.'-w3E[:. Water(TNI)ATC 3%, AR-AFFF forgreenalga(Si,lt,ii(its-itritpirni?(-ortittiintdtelrsit?i)l)ltiecst conditions. This study was conducted accordingto OECD Guideline201 (OE('D 199-1).
I'cstSubstance Test Oroeal nisms Algal Medium
3.0 TES'I'NIE*1'110[)S
Properliev: Aiiiber-coloreldiqtii(sip.ecificEriti-t,%ct. 1.0t)
1).
stable,and conipletelsyoltit)iliei
Te.vctoticentrafioit0s:(a]-2allllCdlLiclolyliirol)3. 500 mg/L.
Soiirce:ATCC 22662
hiocultim preptiriiiiott: Al-al stock was Subcultured 4 days before test initiatioii.
iii01:Ct) ille(iililliI*tlr
U.ve itt test: Stock culture %%-as dillited and spiked lilt()test SOILitioll" to obtain an initial cell density of 1.0 X 10, cells per nil ( 1.2 N IC)4 actual concentration).
Preparatioti:Deionized water %%-asspikedwitlinutrientstocks(includ[112 NA,EDTA) to withinOECD recommended concentrationsT.he most recent chemical analysisfor deionized.-.ateirsin Raw Data Packat-,e.
CliaracteristicsA:t testtime, the mediuni had a pH of 9.06. Sterili/,%e-dia autoclavepriorto u.,ein the test.
Sponsor. 3ArtCompany
Sponsor Study ID# F"-581
3
AS,*lC,irrk,r:ili,5ti,AS@l-Duluili
En%,ir@,iiTicnsetntnaDgli%isii)n
AS,:lSitid-1@[)#5010-069
Exposure Chambers
Incubation
Observations
Endpoint Calculations
7:vpe:Sterilize1d25-ml borosilicate-glaEsrsienmeyer flask-s,eated%vith foam stoppers.
U.veiiitest:50 nilof algalniedium or testsolution/chaiiiberS.ix control replicateasnd threereplicatepser testsubstanceconcentration'A-ere employed. Dufinc,the test,keptstoppered,exceptwhen experimental observationswere made. Solutionswere not aerateddurinc,t,he CXfX)SLires.
Ditration:4 days.
Dailypht)t(perit)iClo:ntinuousat 400 ft-candieussingcot-)]l-i,i.i@e fluorescenltamps. 'restchambers heldon shakertabLleset itI(X)ri)iii.
Tei?ipertitt2i5r.e0: 2@C. Biological:Daily cellcotitittsisin-d,irectmicroscopicCilLiii,,cri%tit-i,Liiii Improved Neubauer heiiioc\.tt)iiieter.
@V(itecrliettiivi(n1.):At testinitiatiaonnd terniinall(Ii)iHi.-CO[ILILLCiItI\\, and temperature,(2)daily-temperature.All determination\%,kerieii:tde accordingtoAPHA itiethod(s1995). 1-,2-,3-,and 4-d EC,,,'sa.nd 4-d NOEC based on botilCellCOLlilainsd averagespecifiocrowt[irates(,u).Algicidal/alcistdaettiecriiiiiii\i%t,iatsiii made posttoxicittyesttermination.
Sponsor. 3161Company
Sponsor Study IDO P-'591
4
ASCI
Dultitit
Fnvir-inrnciTlct%jtlingDi%isiiin
AS,:lSi,.dyIDO 5OlO-Ob8
4.0RESULTS
Test SubstanceToxicity(Pertinendtataare inTables 1,2, and 3)
Test Conditions
CellCt)itt1i-td EC,,: 139 iici@@oil@(117-16-5). ti1-dEq,: 171 ingl,'(L146-199)-
CellCot4ni2-d EC,,,:153 ii2-d EC,: 290 nig,'[(-2i4-330).
CellCo"nt 3-d EC,,,:188 ingi'L(173-'-'10.-1 IA3-d Eq,,):275 mg,L (252-3(X)).
CellCt)iin4i-d ECs,,:141 Iloi,I,-@(12 1t,,I ,u 4-d EC5,,: 294 iii-l@t- ('-'65-326).
Ci,il Cotipit 4-d %70EC: ', I .
I
it 4-d NOEC: 12i iilc@o I..
Effectof testsubstaiic%e%;idseteriiiiiiteodtic algistatibcased on reco%-erovf cellsexlx)sedtt) 500 mg/L concentration.
Tet?ipt-ratt(i'rCt),:
L@@hiititeitsiry.ft-c4-(dXl)v.:
pil: 7.48-9.06.
Specificcondiictit-itv
137-15'-'.
Spon;or: 3161,Company
SponsorStudyID# PI-581
5
AS,:] En@ir,,iiii@viiiii
AS,:lStuj@ 1[)#5010 OtS
';.T0EST QA/QC
QA/QC Cri,,eria
Algalcountsincontrolmust increaseby at leasta factoorf 16 duringthefirs3t-d of the exposure.
Testdurationmust be 4-d.
Data
During thefirs3t-d of thetestcontrolal,,al countsincreasedby a factorof approximately 39.
The testdurationwas 4-d.
6.0 REFERENCES
AmericanPublicHetithAssociatio(nAPHA). 1995.Standard%lettitxfits)irtiIc:xaiiiiiiioili'(iii Water and Wastewater.APHA, Washington,D.C.
OrganizatiofnorEconomic Cooperatiotaind De%-elopinet(i0t1-Cl)).IL)930.1--Cl)(iuilielitiLi)ers Testingof Chemicals.OECD PublicatioInnformatioCnenter.Washinc-tonD..('.
Hamilton,M.A., R.C. Russo,and R.V. Thurston.1977. Trimmed Spearniall-Karb%eIretllod forEstimatingMedian LethalConcentrationisnToxicityBioassivs.Erl%,irkS)cri.Tc,,:Iltlol. 11(7)7:14-719;Correctio1n2 (4):417 (1978).
West, Inc.1996. ToxstatSoftwarePackageVersion3.5.
Spons.or3:NICompany
Sp4)nsorStudy ID# P-1581
6
AS,:lC,)rlx)ratlAo&lIl Dtilutii Te@iiiii:
AS,:lSt-d@ IDP 5010 0(,S
TABLE 1. EC,'S, 95% ConridenceIntervalsa,nd 4-d NOECIS rrom AlgalGrowth Iiihibitioll Testwith FC-603EF Light Water(TM) ATC 3%, AR-AFFF
Endpoint Cell Count
Time
1-d EC@ 2-d EC;., 3-d EC,i 4-d EC,, 4-d NOEC
Data-
(mg/L) 139 153 188 141 31
95 % Ct)nl-iden,:c (nijl)
ll'-I(,5 1
1'14- 1
EndFx)int
Averai!e Specific Gro%%,IhRate (;A)
Time I-d EC@, 2-d EC,, 3-d EC,, 4-d EC,, 4-d NOEC
Data-
171 290 275 294 11-5
95'i @ll I
i -3 NtitAppli --thl-
Values Based on Nominal Test Concentrations
Spunm)r: 3.kfCompany
Sponsor Study [D# P-1591
7
AS,:[
-\S,iD,,[,,Ili
AS,:lStiidkli),5/010068
TABLE2.
Cellspertnland PercentagDeifferencferom\leanControlValueforSelenasti7itit capricorntilEuxmposedtoFC-603EFLightWiter(T.\I)A,TC 3@'tA,R-AFFF
TestConc. (mg/L) Contrt)l
31.25 62.5 125 250 Soo
Replicate
A B C. D E F \Ie@in
A 13 c .\Ie@in
A B c %lean
A B c .\lean
A B c Mean
A B c Ni can
Id
8.5 (-1 8.0(-)
- I-) 5.5 6.0 9.i 7.5u
-T
Cell.-i.('10r4)nl
2d
24 (-) 25(-)
28 37 27 1-7.
-3 -1 49 t-) 4S 39 5.i 43 4(@ 4 1
8.5 (13)" 7.0 (-7) 8.0 (7 @ 7.83 (4)
17 i-19@ 1()1-ill -'2 (--,)1 ),)-.'(-.II)
5'4(1f 41 4(1.11
10 (33) 6.5 (-13@ 5.5 (-2,) 7.33 f-I-)
11)(-311 17 ( 401 22 (-2-1) 19,(@1-31)
-45 -4) 4,;1-4) 44 -.i) 4.4.3(-4)
3.i 1-53) 1.0(-87) 4.0(-47) 2.83 (-62)
20 (-30) IS (-37)
15 1-48) 17.2 (-38)
43 (-S) 39 (-1(1) 3S (-IS) 39.S (-14)
3.0 (-60@ 2.5 (-67) 2.5 (-67) 2.67(-64)
S.i (-69) 5.5 (-SO) 10(-64) 8.00(-71)
12 (-75) lu (-78) 9.5 (-So) 10.3 (-79@
2.0(-73) 0(-Ioo) 3.0(-60) 1.67 (-78)
2.3(-91) 1.5(-95) 3.0(-S9) --).3(3-92)
2.0(-96) 1.5(-97) 1.3 t-97) 1.67 (-96)
i (-I
I I")
'4 54 1 ;1 @l,) 4(,(-34) 4(,(-14) 40.' 1-3-')
14 1-St)) -1((1-72) 14 (-80) 1i.7 (-77)
2.(@4-97) 0.,)(-99) 4.0 t-94j
17 t-97)
Control ReplicateC Values Omitted iL-a;n Outlier b ParentheticValues = % Difference from C4)ntrolNlean Value
0-d %lean Algal Count 1.2 X 10,
..Sponsor; 3,%lCompanv
Sponsor Study ID& PI-@ST
8
ASci C,irpiriii,,AnSci-Duluth L-'ii%irtinritenTtci.l%tingDj%isj4iii
AS,:lSluJ% IDZ 5010-0tS
TABLE 3. Average SpeciricGroulh Rates (,ua)nd PercentageI)irrerencreroni%leiiiControoll Value forSeknastrum capricornutumExposed to FC-603EF l.ighl%%ater(l'.NAlR)AFFF
Test Conc. (mg/L) Conin)l
31.25 62.5 125 250 500
Replicate
A B C, D I.F ,%Ican
A B c Nicaii
A B c .%lean
A B c Nlcafi
A B c Mean
A B c Mean
1.958 1.897
1 522 I,bw 2.069 1 833
1.959 (711.764 ( 4) 1 897 (4i I 8"t, 1-1
]'-0(lb) 1 689 1-81 1.522 (-17) 1.810(-1)
1.070 1-42) 0.182 1-100) 1.204 (-34) O@859 (-53)
O@916 (-50) 0.734 (-60) 0.734(-60) 0.799(-56)
0.511 (-72) 0(-Ioo)
0.916(-50) 0.329 (-82)
.p =
In Cells per mi t-- In Cell%/mi I t.- L,
Control ReplicateC Values Oinittedas in Outlier
ParcnUm:LicValues = % Difference from CtinirL)%llean Value
0-d Mean Algal Count = 1.2 X 104
Sponsor. 3161 Company Sponsor Study ID# F"-581
Avem@c Specific GnvAih Rite 4 r
2d
3J
1 @487 1.509
- I-) 1W f 1 707 1 547 1 '.S70
1 325 lb, 1 3tg 13) 1 454 1 385 i !Zt
1 233
189 I zis
1 381 12)
1,311 17)
1 443 81
01
1 @381 1
1 394 t-Ili l@340 ( 15@ 1 246 1-21) 1 330 1-15)
0,979 1-38; 0761 ( 521 1.060(-32) 0.949(-40)
is') 1 iw 1 is, i@ 1 !C)7 1-4!
0 75 -,S. 0 707 '4'-) 0 690 4-431 0.718 (411
0.367 (-77) 0.112 t-931 0.458 1-71) 0.332 1-79)
O@ 170 ( 9846,)) 0.074 1 0,074 10 1]01
4d
0 )7S 0 1)8.1
0 os-, 04@,
101!
0 ),)S I
0
U,017 S. o').-, 0 _, : E, 0,)41 71
0927 i 9 1 01)01) 10@ 0,)0-) 10, 0915 91
ot,], 0 o,)," I 0605 :-40)) 0.642 -3o
00,128 ( 871 030119 1-100)
1-70) 48 4-851