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AR286 -- 0708
MICROBICS MICROTOX TOXICITY TEST
TEST SUBSTANCE
Identity:
A mixture containing referred to as PFOS,
perfluorooctanesulfonate, which may also FC-95, or as a component of FC-201F-X.
be (1-
Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot numberwas 1. The test sample is FC23061%F-dXie(tFh-y1l1e1n2e9g)l.ycColurbruetnylt ietnhfeorr,ma3t6i.on6%inwdaitceart,es8i.t2is5%a macirxytluircefo3a%mePrF,O7S%, s0o.d1i5u%mtoolcutoylltrsiualzfoaltee., 6% sulfonated alkyl ether salts, 3% triethanolamine, and
cThhaerafcotlelrowiiznegd scounmcmeantrryataipopnlsioefs itmopaurmiitxiteusr.eDwaittha imnacyomnpoltetacecluyrately reflect the toxicityof the fluorochemical componentofthetestsample.
METHOD:
Method: Microbics Microtox Procedure
TGeLsPt:tyNpoe: Acute static
YSepeacriCeosm:plPehotteodb:ac1t9e9ri2um phosphoreum
AEnxaploystiucrael pmeorniiotdo:ri3n0g-:mipnHutaensd osmotic condition.
Statistical methods: EC50 values calculated by statistical linear regression
program provided for Microtox
TTeesstt ocorngdaintiisomnss:ource:
Microbics' Reagent.
.
Diluent: Millipore Mill-QTM water
Test temperature: Not noted.
Sptreopcakreadndbyteasdtdsionlgu2t0io0nmpgroefptaerasttimoant:erAialprtioma1r0y0smtLockMislolliuptoiroenMwilais-QTM
water. The pHof the stock solution was 7.4. An osmotic adjustment was
tmeastdesoulustiinogns20w0asmgnoNtaedClasdi"scsloelavreadnidn c1o0lomrlLessst"o.ckAlsotleusttiosno.luAtpiponesawrearnece of
made by proportional dilutions.
Exposure vessels: 4 mL cuvettes.
NNuummbbeerr ooff rceopnlciecnattreas:tiotnwso: Four plus a blank control
-
Element basis: Percent light loss
RESULTS
Nominal concentrations: Blank control, 125, 250, 500, and 1000 mg/L.
000388
Element value:
5-minute ECso = 153 (130 -- 180) mg/L
15-minute ECso = 135 (108 -- 170) mg/L 30-minute ECso = 110 (84 -- 145) mg/L.
Element values based on nominal concentrations.
Remarks: Testing was conducted on a mixture as described in the Test
Substance Remarks field. The values reported apply to that mixture and not
the fluorochemical proportion alone.
conclusions
`The test substance 30-minute ECs for Photobacterium phosphoreum was determined to be 110 mg/L with a 95% confidence interval of 84 to 145 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133
batAQuauty
00000
Reliability: Kiimisch ranking 2. Testing meets all criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amountoffluorochemical proportion in the: solution.
REFERENCES
This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number K1126-1, 1992.
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Last changed: 6/26/00.
000359
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Environmental Lab -- Work Sheet F-mzq wrt
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NH NUMBFR EIRNALARESACUOLNH TGS. GAMMA= mGiAH MnMA EST. 7% EFFECTA K1126R-A1.SK5
2| 3
2115230551.1000000000000
001...77479800906% | 00T..8s9558e53
4443..159033 ezisie
3< .
2523000001.1000000000000
31..07013468 2.3126
21.175433068 2.7802
e7s5..i09205 74.003
7: 1000000..00000000 44..387178 44..a5v9e6d4 oBi2..328040
EC 50 = 152.6346 ma/L (957% CONFIDENCE RANGE: 129.7237 TO 179.5920)
H CCC0H ToucH h SPAA CE BAR to Continue...H CCC0CECC00ECLLLLLLE0I 0LEELLLLLlEEitLLLLll
000391
.
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MICROTOX DATA REPORT
FILE NAME: K1126-1.K5 Sample Descriotion:
TEST DATE: TEST TIME:
FC - 201F-X F11125 LOT 1 BROWN LIQUID TEST DATE 4/17/92
PIrnoicteidaulreC:oncBeAnStIrCation : 1000 mg/L Test Time: S minutes
ODsimlouttiiconAFdajucsttomren:ts2 NaCl Concentration Units: `mg/L
NUMBER 10/1T
cone.
GAMMA
1 2
86/ 42 87/ 42
125.0000 125.0000
3 a
84s 27 88/ 28
250.0000 250.0000
s 6
88/ 19 86/ 19
500.0000 500.0000
7 8
8971// 1144 11000000..00000000
0.770004 0.790594 1.689314 1.71675 3.003634 2.912644 44..367118776348
CONTROL IT/I0 = 80/ 94.
BLANK RATIO = 0.8644
10 3ZSDLDODPDEDDD=DD0D.8D3D7D4ODDDDDDDDDDDDDDDDDDDIDDDDDDDDDDDDDDDDDDDDDDDDD?3
7 3
f-- =
3 3
63 A3
5
3
3
mo no
&
3
3
a3 3
az
3 3
d3zzrzzzzzzzzzzzzzzzzzz.z
< 3
3 3
|
Tz.
_--
3 3
3 3
. .
3 3
3
.
3
.
3 3
3 3
LEC 50 .
3 3
+10 G 10 DDODDDD100D.D CODNODCDEDNDDTDRDD AOTDIDDODNDD D1D0DO0D0DD DDDDDDDDDDDDDDDDDD DDD1DD0Y84
EC SO = 152.6346
(95% CONFIDENCE RANGE: 129.7237 TO 179.5920) # Used for calculations
000392
Ty 410
STATISTICAL REPORT FOR DATA FILE K1126-1.KS
ESTIMATING EQUATION: LOG C = 1.1702 x LOG b NUMBER OF DATA PAIRS USED:
RESIDUAL VARIANCE: 0.00303 95% CONFIDENCE FACTOR: 1.17661 FOR CSO CORRELATION COEFFICIENT: R = 0.58991
+2.1837
EFFECTIVE CONCENTRATIONS (95% CONFIDENCE RANGE):
EECC 5200 == 13502l.16539416 mmoo//LL EECC 10i== 1o1l.67608S4Immeo//LL
((12209..67424397 TTO0 14739..55972703))%4% ((70..02780170TO 19.i.275z579z)re%e4sx
XX EXTRAPOLATED RESULTS
000393
top own
HHHHFINH AL H RESUHLTSHHHHHHHH BHHH HHH HHH K112R6H-A1.KH1H5 HA 1
NUMBER
CONC.
GAMMA GAMMA EST. % EFFECT
21
112255..00000000 00..88925112
00..99560044
47.231 45.092
3 a
250.0000 250.0000
11..99340775
11..77221122 6665..087739
s 6
500.0000 500.0000
3.3345 3.5008
3.0847 3.0847
76.929 77.782
7 8
1000.0000 1000.0000
5.3499 4.6038
5.5281 5.5281
84.252 82.155
:
EC 50 = 135.4463 mg/L (95% CONFIDENCE RANGE: 108.2013 TO 169.5515)
HHLHIHCTHouHchHSHPAHCEHBHARHto HcAoHnHtiHnuHe.H..O.[HCCHCHCCHCHCCH CCOHCCCHCHC0HCHCrHCERrEHrOEeHrEHerrHecHerHrHrrr
000394
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MICROTOX DATA REPORT
FILE NAME: K1126-1.K1S Sample Description:
TEST DATE: TEST TIME:
FC - 201F-X F11129 LOT 1 BROWN LIGUID TEST DATE 4/17/92
Procedurs: BASIC Initial Concentration : 1000 mg/L Test Time: 15 minutes
ODsimlouttiiconAFdajucsttomren:t:2 NaCl Concentration Units: mg/L
NUMBER 10/1T
cone.
GAMMA
|
t 86/ 38 125.0000 2 87/ a0 125.0000 a3 8884/s 2254 223500..00000000 s 88/ 17 500.0000 6 86/ 16 500.0000 7 91/ 12 1000.0000 8 87/ 13 1000.0000
0.895064 0.821244 11..994370478344 3.334534 3.500774 5.349928 3.603824
CONTROL IT/10 = 77/ 94
BLANK RATIO = 0.8374
10 Z3SDLDODPDEDDD=DD0D.D8D4D1O7DDODDDDDODDDDDDDDDDDIDDDDDDDDDDDODDDDDDDDODDDD?3
3 3
7
3 3
53 aA 3
68
33
Moz Moz
3 3
A3 3
4
3 3
1 >zzzzzzzzzzzzzzzzzzzzz_._
3
iz
< 3
3 3
_-- .
3 3
3 3
--
. .
3 3
3 3
. .
3 3
3. 3
LEC S0 :
3 3
+10 G10DDDDDDODDDDDODDD1D0D0D .CDOONDCDEONDTDRDADTDIDODNDDD1D0D00DDDDDDDDDDDDDDDDDD1Y084
EC SO = 135.4463 (95% CONFIDENCE RANGE: 108.2013 TO 169.5515) # Used for calculation
000395
Vey 1k U
STATISTICAL REPORT FOR DATA FILE K1126-1.K1S
ESTIMATING EQUATION: LOG C = 1.1494 x LOG b +2.1318
NUMBER OF DATA PAIRS USED: 8
RESIDUAL VARIANCE: 0.00492
95% CONFIDENCE FACTOR: 1.25180 FOR ECSO
CORRELATION COEFFICIENT: R = 0.98358
EFFECTIVE CONCENTRATIONS (95% CONFIDENCE RANGE):
EC EC
SO 20
= =
12375..54246663
mmgg//LL
EC EC
10 1
= =
100..68838860 mmgg//LL
( (
11068..82902143
TO TO
~14649..85555152))%%%
(( 05..26137655 TTOO 202..81379988))%kx*x
#Xx EXTRAPOLATED RESULTS
000396
3g _A NOMEFEIRNAL RESUCLONTGS. GA3e-GAmMAnET. EFFECT(1126-1.K30
33i 2i153z05.l.000000000000 | o21.l.3e90a3me0 | 21lli.os3ee3s 5a70vo..e56is%e ::i SS20o5000iii000000000000 243..32007094%19 35s..l00e35s5 ra7ee9i..8sg9ie9es &3 1i300o0o0o0c0000 eS.iceis5sz e65ie9m6s 6o9s.i7easss EC SO = 110.3339 mo/L (75% CONFIDENCE RANGE: 83.8096 TO 145.2529) CEA CTouchA SPACE BAR tocontinue. COCecR rteA etLlH tLitA LeeeAtetAeeeteerettrers
000397
Rs BLE
MICROTOX DATA REPORT
FILE NAME: K1126-1.K30 Sample Description:
TTEESSTT DTAITMEE::
FC = 201F-X F11129 LOT 1 BROWN LIQUID TEST DATE 4/17/92
PIrnoicteidaulreC:oncBAeSnItCration : 1000 mg/L Test Time: 30 minutes
ODsimlouttiiconAdFjaucsttomren:t:2 NaCl Concentration Units: ma/L
NUMBER 10/IT
cone.
GAMMA
.
2t 8876// 3376 112255..00000000
a3 8848s/ 2221 225500..00000000
s 6
88/ 86/
15 14
550000..00000000
7 8
91/ 87/
11 11
11000000..00000000
01..909235972644 22..339911996644 43..290794088844 56..700165813944
CONTROL 1T/10 = 79/ 94
BLANK RATIO = 0.8480
10 32SDLDODPDEDD=DDD0D.D8D3D8D4DODDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDODDDDDDDDDDD?3
3 [I 3
8
3 3
6=
3
a3 M3
_s
3
3
AM33
_a
3 3
3 >zrzzzzzzzzzzzzzzzzz 1
3 <
3 3
.2
3
.
3 3
3 3
: .
3 3 3
3 3
. .
3 3
+10 33@DDDDDDDDDDDDDDDODDD"..-DEDCDDSDODDDDDDDDDDDDD~DDDDODDDODDDDDDDDDDDY33
10
100 CONCENTRATION 1000
10E4
EC 50 = 110.3339
(95% CONFIDENCE RANGE:
83.809 TO 145.2529) # Used for calculations
000398
yr STATISTICAL REFORT FOR DATA FILE Ki126-1.KS0 ESTIMATING EQUATION: LOG C = 1.1478 x LOG b +2.0427 NUMBER OF DATA PAIRS USED: RESIDUAL VARIANCE: 0.00549 95% CONFIDENCE FACTOR: 1.31648 FOR ECSO CORRELATION COEFFICIENT: R = 0.98097 EFFECTIVE CONCENTRATIONS (95% CONFIDENCE RANGE): EEECCCS21000 === 12128001.483763803139 mmmega//sLLL ((( 4.61322..0870979706 TTTOOO . 1341958...32e45a9207950))5580s8rk% EC f= 0l5652 ma/L (0.1546 TO 2.036884 Xk EXTRAPOLATED RESULTS
000399