Document XONXLeoMzJZZkREV5n6y9Z41g
& 3M ENVIRONMENTAL LABORATORY
METHOD
EXTRACTION OF POTASSIUM PERFLUOROOCTANESULFONATE OR OTHER FLUOROCHEMICAL COMPOUNDS FROM URINE FOR ANALYSIS USING HPLC-
ELECTROSPRAY/MASS SPECTROMETRY/MASS SPECTROMETRY
Method Number: ETS-8-96.0
Adoption Date: 7-28-44
Author: Lisa Clemen, Glenn Langenburg
Revision Date: NA
Approved By:
\ 1] Ze
Laboratory Mhnager
Date 5/77
Lit hn
Group Leader
28/95
Date
on A Cloman Technical Reviewer
Hara Date
10 SCOPE AND APPLICATION 11 oSrcooptehe:r Tfhliusormoecthehmoidcails fcoormtphoeuenxtdrsacftrioomnoufrpinoet.assium perfluorooctancsulfonate (PFOS) 12 Applicable compounds: Fluorochemicals or other fluorinated compounds. 13 Matrices: Human, rat, and monkey urine or other fluids as designated in the validation
report.
Word 695
ExtrEaTcoSft-Pi8FoO9Sn6f0om Urine
001045 Page tofis
2.0 SumMMARY OF METHOD 2.1 (ThPiFsOSm)etohroodthdeerscfrliuboersotchheempircoaclesdufrreomfourrienxet,raocrtiontgheproftlausisdsi,umuspienrgflaunorioooncptaainreisnuglfroenaagteent
PanFdOSm,ethPyFlO-tSeAr,t-bPuFtOylSAetAh,erE(tVFIOBES)E.-OIHn,thMi5s5m6e,thMo5d7,0e,igphetrfflluuoorrooocchteamniocaatles (aPreOAexAt)ra,ctaendd: ssaurmrpolgeataensdttahnedaarndal(ysteee f3o.n0 pDaeifriniistpiaorntsi)t.ioAnend iionntopMaiIrBiEn.g reTahgeenMtIiBEadedxetdratcottiwsoremmlovofed maentdhpauntolo,nttoheannfiitlrteorgeedntehvraopuogrhatao0r.u2ntyilmdnryy.lonEafcilhteerxattratcatchiesdretoco3nsctcitpultaesdtiicns0y.r5inmgleoifnto glass autovials.
22
These sample method.
extracts
arc
analyzed
following
method
ETS-8-97.0
or
other
appropriate
3.0 DeFNITIONS 31 PFOS: perfluorooctanesulfonate (anionofpotassium salt) CF,,SO; 32 PFOSA: perfluorooctane sulfonylamide C,F,,SO,NH, 33 PFOSAA: perfluorooctanc sulfonylamido (ethyDacetate CF,,SO;N(CH,CH,)CH,CO; 34 EtFOSE-OH: 2(N-ethylperfluorooctane sulfonamido)-thyl alcohol
CF,SO.N(CH,CH,)CH,CH,OH 35 MSS6: CF,SO,N(H)(CH,COOH) 36 MS70: CF,SO,N(CH,)CH,COOH
37 POAA: perfluorooctanoate C,F,,COO' 3.8 Sumogate standard THPFOS: 1H-1H-2H-2H perfluorooctane sulfonic acid, used as an
intemal standard in this method.
4.0 WARNINGS AND CAUTIONS 41 Health and safety warnings
4.1.1 Use universal precautions, especially laboratory coats, goggles, and gloves when handling animal tissue, which may contain pathogens.
50 .0 Inve0 meeme0 nces0000000000
5.1 Athtatthmisaytibmee,pirteissenutnksuncohwnashcoownjtuhgeateexdtrfalcutioornocmheetmhicoadlsis(aefgf.eGcltuecdubryonpiodteesn)t.ialCoinntjeurgfaetreesnces
may become deconjugated during reported resultsof target analytes.
extraction
or
analysis
resulting
in
a
high
bias
for
ExtractionEoTfS.P8FO.S960from Urine
Page 20114
001046
6.0 EQuipMENT 6.1 aTchceepftoalblleo.wing equipment is used while performing this method. Equivalent equipment is
6.1.1 Vortex mixer, VWR, Vortex Genie 2 6.12 Centrifuge, Mistral 1000 or [EC 6.1.3 Shaker, Eberbach or VWR 6.1.4 Nitrogen evaporator, Organomation 6.1.5 Balance (0.100g)
7.0 SUPPLIES AND MATERIALS 71 Gloves 72 Eppendororf disposable pipettes 73 Nalgene bottles, capable ofholding 250 ml and 1 L 74 Volumetric flasks, glass, type A 7.5 L.CHEM vials, glass, 40 ml glass 7.6 Centrifuge tubes, polypropylene, 15 ml 77 Labels 7.8 Oxford Dispenser ~3.0 to 10.0 ml 79 Syringes, capable of measuring 2.5 kL to 50 uL 7.10 Graduated pipettes 7.11 Syringes, disposable plastic, 3 cc 7.12 Syringe filters, nylon, 0.2 um, 25 mm 713 Timer 7.14 Crimp cap autovials and caps 7.15 Crimpers Note: Prior to using glassware and bottles, rinse 3 times with methanol and 3 times with
Milli-QTM water. Rinse glass syringes a minimum of9 times with methanol, 3 rinses from 3 separate vials.
8.0 REAGENTS AND STANDARDS 81 Type reagent grade water, Milli-QTM or equivalent; all water used in this method should
beMilli-QTM water and may be provided by a Milli-Q TOC PlusTM system
82 Sodium hydroxide (NaOH), J.T Baker or equivalent 83 Tetrabutylammonium hydrogen sulfate(TBA), Kodak or equivalent 84 Sodium carbonate (N&,CO,), J.T. Baker or equivalent 85 Sodium bicarbonate (NaHCO), J.T. Baker or equivalent
ExtractiEonToSf8P.F9OS60from Urine 001047 Page 3of 14
8.6 Methyl-T-Butyl Ether, Omnisolv, glass distilled or HPLC grade 87 Methanol, Omnisolv, glass distilled or HPLC grade 88 Urine frozen from supplier
89 Fluorochemical standards
89.1 PFOS (3M Specialty Chemical Division), molecular weight = 538 8.9.2 PFOSA (3M Specialty Chemical Division), molecular weight = 499 8.9.3 PFOSAA (3M Specialty Chemical Division), molecular weight = 585 8.9.4 EIFOSE-OH (3M Specialty Chemical Division), molecular weight = 570 8.9.5 M56 (3M Specialty Chemical Division), molecular weight = 557
8.9.6 MS70 (3M Specialty Chemical Division), molecular weight = 571 89.7 POAA (3M Specialty Chemical Division), molecular weight = 452 89.8 THPFOS (1-H,1-H, 2-H, 2-H C/F,,SO;H) molecular weight = 428 8.9.9 Other fluorochemicals, as appropriate.
810 Reagent preparation
NOTE: Wprheepanraptrieopna,riadnjgulsatragcercovrodlinugmleys.than listed in reagent, standard, or surrogate 810.1 11000N0 msoldbieuamkehrydcroonxtaiidnei(nNga5O0H0)m:lWMeiilglih-QaTMpprwoaxtiemra,tmeliyx2u0n0tilgaNllaOsoHl.idsPoaruer: into a
dissolved. Store ina 1 L Nalgene bole.
8102 N1aNOsHodsioluumtihoyndriontxoidae1(0N0amOH!)v:oluDmielturtiec1fl0NaskNaanOdHdil1u:t1e0.toMveoalsuumreeu1s0imnglMoifll1i0N QM water. Store in a 125 ml Nalgene bottle.
810.3 0o.f5TMBAteitnrtoabaut1 yLlavmomlounmeiturmihcycdornotgaeinnisnuglf5a0t0e (mTlBMAi)l:liW-QeTMighwaatpeprr.oAxdijmuastteltyo p16H9 10 NusaiOnHg ,apapddrosxliowmlaytbe4elc4yautsoe5t4hemlpHofc1h0anNgeNsaaObrHupt(lWyh).ileDialdudtientgotvhoe lluasmtemwlitohf Milli-QTM water. Store ina I L Nalgene bottle.
8.10.3.1 TneBeAderdequusiirnegs 1aNchNecakOpHrisoorltuotieoanc.h use to ensure pH = 10.0. Adjust as
8.10.4 0a.p2p5roMximsaotdeiluym2c6a.r5bonoaftseo/sdoiduimumcabribcoanrabtoena(tNea,buCfOfje)r(aNnad,2C1O.0/NgaoHfCsOo,d)i:umWeigh
bicarbonate QM water.
(NaHCO,) Storeina |
iLntNoaalg1 eLnevoblotutmlee.tric
flask
and
bring
to
volume
with
Milli-
8.11 Standards preparation
8.11.1 Prepare PFOS standards for the standard curve.
8.11.2 Pflrueopraorcehoetmhiecralflsutoarnodcahredmsiacrael asctcaendpatradbsl,e a(sfoarpeprxoapmrpilatee,.onMeulwtoirckoimnpgosnteanntdard
solution containing 1.00 1.10 ppm EXFOSE-OH.)
ppm
PFOS,
1.02
ppm
PFOSA,
0.987
ppm
PFOSAA,
and
ExtractionoETfSP8F.O9S60from Urine
Pagedof 14
001048
8.11.3 tWheeiagchtuaaplpwreoixgihmtatienltyhe10S0tamngdaordf PLoFgObSooikn.to a 100 ml volumetric flask and record
8.11.4 Bring to volume with methanol for a stock standardof approximately 1000 ppm
(ug/ml).
8.115 aDpilpurtoexitmhaetsetloyck50soplpumt.ion with methanol for working standard 1 solution of
8.11.6 aDpiplruotxe.wo5r.0kipnpgm.standard 1 with methanol foar working standard 2 solution of
8.11.7
Dilute working standard approx. 0.50 ppm.
1
with
methanol
for
a
working
standard
3
solution
of
812 Surrogate stock standard preparation
8.12.1 CWJeFi,g;hSOap;pHrionxtiomaat5e0lyml50v-o6l0ummegtroifcsfulrarsokgaantde srteacnodradrtdhe1-aHc,t1u-alH,we2i-gHh,t.2-H,
8.12.2 Bring to volume with methanol for a surrogate stockofapproximately 1000-1200
ppm.
8.12.3
Prepare stock to
aa s1u0mmoglavtoelwumoertkriincgfsltaasnkdaarndd.
brTirnagnstfoevroalpupmreoxwiimtahtemleyth1amnolloffsourarrogate
S`wtoarnkdianrgdsLtoagnbdaorodko.f100-120 ppm. Record the actual volume transferred in the
9.0 SAMPLE HANDLING 9.1 All samplesarereceived frozen and must be kept frozen until the extraction is performed. 92 Allow samples to thaw to room temperature prior to extraction.
100 QuaLITY ContRoL
10.1 Solvent Blanks, Method blanks and matrix blanks
10.1.1 An aliquot of 2.0 ml methanol is used as a solvent blank
10.1.2
Extract two 2.0 method blanks.
ml
aliquots ofMilli-QTM
water
following this
procedure
and
use
as
10.1.3
Extract blanks.
two 2.0 mi aliquotsofthe See 11.1.4.
urine
following
this
procedure
and
use
as
matrix
102 Matrix spikes
10.2.1 Pthreepaacrceuraancdyoafntalhyezeexmtartarcitxiosnp.ike and matrix spike duplicate samples to determine 102.2 `Pmraetpraixrereccaecihvsepdiwkiethuseiancghassaammpplleesceth.osen by the analyst, usually the control 10.2.3 cEuxrpveec.teAdddciotnicoennatlrastpiioknesssmhaouyldbefailnlcilnudtehde maindd-mraanygefoalfltihnethieniltoawl-craalnigberaotfitohne
initial calibration curve.
ExtacEoTftSP8oF.On9S60from Urine 001049 Page Sof 14
10.2.4 Prepare one matrix spike and matrix spike duplicate per 40 samples, with a minimumof 2 matrix spikes per batch.
103 Continuing calibration verifications
10.3.1 Pinrietipaalrecacloibnrtaitniuoinngcucrvael.ibration verification samples to ensure the accuracyof the
103.2 Psraemppalrees,. atFoarmeixnaimmpulme,, ionfaescaomnptlienusientg=ca3l4i,brfaotuironchveecrkifsicaarteiponreppearregdroaunpdof 10 extracted.
10.3.3
Prepare each continuing prepare the initial curve.
calibration
verification
from
the
same
matrix
used
to
10.3.4 The expected concentrations will fal within the mid-range of the initial calibration curve. Additional spikes may be included that fal in the low-range of the initial calibration curve, This is necessaryifthe analyst must quantitate using tohnalny 5thpeplbo--w e1n0d00ofpptbh)e. calibration curve (for example, 5 ppb -- 100 ppb, rather
11.0 CALIBRATION AND STANDARDIZATION 111 Prepare matrix calibration standards
11.11Transfer 2.0 mlofurine to a 15 ml centrifuge tube. 11.12 Record each sample volume on the extraction sheet. 11.1.3 While preparing a totaloftwenty-two aliquots in 15 ml centrifuge tubes, mix or
shake between aliquots. 11.1.4 Two 2.0 ml aliquots serve as matrix blanks. 11.1.5 Typically use the standard concentrationsandspiking amounts listed in Table 1,
at the endofthis section, to spike, in duplicate, two standard curves, for2 total of twenty standards, two matrix blanks, and two method blanks 11.1.6 RraenfgeerstaonvdaltihdeatLiionneraerpCoarltiFbrAaCtTio-nTROaXn-g1e3(1L,CWR)20f6or7,calwihbircahtiloinstcsutrhveesw.orking 11.1.7 Use Attachment D as an aid in calculating the concentrationsof the working standards. See Section 13.0 to calculate actual concentrations of PFOS in calibration standards. 11.2 To each standard, blank, or continuing check, add appropriate amountofsurrogate `working standardforthe concentration to fall within the calibration curve range 10ppb 1500 ppb. 113 Extract spiked matrix standards following 12.6-12.16ofthis method. Use these standards to establish each initial curve on the mass spectrometer.
ExtractionEoTfSP8FO9S60from Urine 001050 Page of 14
Table 1
Approximate spiking amounts for standards and spikes
Using 2.0 ml ofmatrix
Working standard WL | Approx. final conc. | Approx. final conc
(approx. conc.)
ofanalyte in matrix_| Of analyte in solvent
Bk
Bam |
1.25 ppb
5.00 ppb
0.500 ppm [10 |
0.500 ppm
26.0 .p50pppbb.
21500.p0 pppbb
3.00 ppm
50.0 ppb
[00p 5.00m ppm. [10 | 2625.50pppbb
250 ppb
S00ppm 50.0 ppm
|5
I 2a0S0pppebb| 7S5000ppepbb
500ppm |10| 250ppb
1000 ppb
50.0 ppm.
375 ppb
1500 ppb
12.0 PROCEDURE 12.1 Owabttearibnatfrhozen samples and allow to thaw at room temperature or in a lukewarm 122 Vortex mix for 15 seconds, then transfer 2.0 ml or other appropriate volume to a 15 ml
polypropylene centrifuge tube. 123 Retum unused samples tofreezerafter extraction amounts have been removed. 124 Record the initial volume on the sample weight/volume worksheet. . See Attachment D.
`The original weight/volume worksheet s included in the study binder. 12.5 Label the tube with the study number, sample ID, date and analyst initials. See attached
`worksheet for documenting the remaining steps. 12.6 Spike all samples, including blanks and standards, ready for extraction with surrogate
Standard as described in 11.2. 127 Spike each matrix with the appropriate amountofstandard as described in 11.1, or Table
Tin that section, for the calibration curve standards. Also prepare matrix spikes and continuing calibration standards. 128 Vsoarmtpelxesmifoxr t1h5essetcaonnddasr.d curve samples, matrix spike samples, and continuing calibration 129 Check to ensure the 0.5 M TBA reagent is at pH 10. I not, adjust accordingly. 1210 To cach sample, add 1 mi 0.5 M TBA and 2 ml of 0.25M sodium carbonate/sodium bicarbonate buffer. 12.11 Using an Oxford Dispenser, add 5 ml methyl-tert-butyl ether. 12.12 Cap each sample and put on the shaker at a settingof 300 rpm, for 20 minutes.
ExtractionEoTfSP-F8O9S60from Urine
001051 Page of 14
12.13 Centrifuge for 20 to 25 minutes ata settingof 3500 rpm, or until layers are separated. 12.14. Labela fresh 15 ml centrifuge tube with the same information as in 12.5. 12.15 Remove 4.0 mlof the organic layer to this clean 15 mi centrifuge tube. 12.16 Put each sample on the analytical nitrogen evaporator until dry, approximately 30 to 60
minutes. 12.41 Add 0.5 mlofmethanol to cach centrifuge tube using a graduated pipette. Ifexcessive
residue is present, add the methanol and allow the extract to sit for 30 minutes prior to vortexing
12.17 Vortex mix for 30 seconds 1218 Lmaatbreilx,thfeinaaultosovlivaelnwti,tehxttrhaecstitoundydantue,mbfelru,oraoncihmeamlicnaulmcboemrpoannedntgesn,deerxt,rsacatmipolnettyipem,epvoiianlt,
file archive number, and analys(s) performing the extraction. 12.19 Attach a0.2 jum nylon mesh filter to a 3 ce syringe and transfer the sample to this
syringe. Filter into a 1.5 mi glass autovial or low-volume autovial when necessary. 12.20 Cap and store extracts at room temperature or refrigerated at approximately 4 C until
analysis. 12:21 Complete the extraction worksheet, attached to this document, and tape in the study
notebook or include in study binder, as appropriate.
13.0DATAANALYSISANDCALCULATIONS
13.1 Calculations 13.1.1 Calculate actual concentrationsof PFOS, or other applicable fluorochemical, in calibration standards using the following equation:
mi ofstandard x concentrationof standard (ug /ml)
=
ml of standard + mlof surrogate standard + initial matrix volume (al)
Final Concentration (g/ml) ofPFOS in matrix
14.0 METHOD PERFORMANCE 14.1 TfohrespmeectihfiocdMdeDtLectainodn lliimmiitto(fMqDuLan)tiitsaatniaolny(teLaOnQd)mavtalruiexs s(pseeceifAitc.taRcehfmeerntto BM).DL report 142 The following quality control samples are extracted with each batch of samples to
evaluate the quality of the extraction and analysis. 14.2.1 Method blanks and matrix blanks. 14.2.2 Matrix spike and matrix spike duplicate samples to determine accuracy and
precisionofthe extraction. 14.2.3 iCnointitailncuailnigbrcaatliiobnractuirovne.check samples to determine the continued accuracy of the 143 Refer to section 14of ETS-8-97.0 for method performance criteria.
ExtractiEonoTfSP8F.O9S60from Urine
Page of 14
001052
15.0 POLLUTION PREVENTION AND WASTE MANAGEMENT
15.1 Halul motahnerasnadmmpolenkweaystseaimspdliespwoassetdeiins dnoinsipnofseecdtiionuisnfbeicothiaozuasrbdiowhaasztaercdonwtaasitneersc.ontainers,
Flammable disposed in
solvent broken
gwlaasstsecoisntdaiisnpeorssedloicnatheidghinBtTheUlcaboonrtaationreyr.s.
Used
glass
pipette
waste
is
16.0 RECORDS 16.1 nCootmepbloeotkeotrheinecxlturdaectiinonthweo3r-krsihnegesttautdtyabcihnedderto, tahsisapmpertophroida,tea.nd tape in the study
17.0 TABLES, DIAGRAMS, FLOWCHARTS, AND VALIDATION DATE 17.1 Attachment A, Extraction worksheet 172 Attachment B, MDL/LOQ values and summary 173 Attachment C, Calibration standard concentration worksheet 174 Attachment D, Sample weight/volume worksheet
18.0 REFERENCES 18.1 The validation report associated with this method is FACT-TOX-131, W2067.
19.0 AFFECTED DOCUMENTS
19.1 `ETCSo-m8p-o9u7n.d0s, in"AUnrailnyesiEsxotrfaPcottsaUsssiinugmHPPeLrCf-lEuloreocotcrtoasnpersaulyfMonaastse SorpeOctthreormFelturoyr/oMcahsesmical
Spectrometry"
20.0 Revisions
ReNvuimsbieorn
Reason For Revision
ReDvaitsieon
ExtractiEonoTfSPF.O9S6f0romUrine 001053 Page 90f 14
Extraction Worksheet ETS-8-96.0
SMuadtryix7
SoApmporogxa.e Spdpm| apFpEr0M.o5pxo.m| apFpMr$pop.m | approFx.C$M0ppm| Commens
BoWxha
fiActual ppm| "sctal ppm| "cual ppm |'senal ppm
DateSpiked Anlyst
eo rr
7
-- w wo or
-- -- r rr r
r rr r r r r
r rr r r
---- rr
7 fr]
--r -- r--r --r r r1
r rT T ]
r rr rr r 1
-- r -- rr 0r r T
r rr rr r
rT
r --r T r
r rr r r
7/1
f Ie i (a Lt,
[Bak
saw
emewm=
ml
|
ser
[S Sikemmn ere]
rma
Doned0eec
1]
`Cont. Cal. Verifications usedsamematrixas for sd curve.
Auachment A
EEox TfSF.8O.S96f0c omUne 004.054 Page 100114
MDL/LOQ values for human urine Linear Calibration Range (LCR)
FOS [(ppb4 ) ||1(6 p4p7b)| 15 ppb 1500 ppb (in final MeOH extract)
PFOSA
5205 ppppbb--- 11050000ppppbb((iinnffiinnaall MMeeOOHHeexxttrraacctt))
PFOSAA.
EFOSE-OH
2255 ppppbb -- 11000000 ppppbb ((iinn ffiinnaall MMeeOOHH eexxttrraacctt))
M556
25 ppb - 1000ppb (in final MeOH extract)
M570
25 ppb- 1000ppb (in final MeOH extract)
d= not determined
NOTE: to calculate MDL, LOQ, and LCR values in ug/miofurine divide the above values by 4.
MthDesLe/mLatOrQicveaslwueesreinexrtatraacntdedmaonndkaenyaluryizneedwweirteh nthoet hstuamtiastnicuarlilnyedceuterrvmeisnteod.detTewromicnuerevqeusivinaleeancche.of Rareespaosnssuemsedinttohbeerastimainldarmotoritkheeyvawleureessdiemtielramritnoetdhefohruhmuamnanreusrpionnes.es, therefore, their MDL and LOQ
Ifasuitable amountofclean, control matrix i available, samples will be evaluatedversus a curve extracted from urine originating from the same species as the specimens.
Please see LOQ Summary and MDL study in FACT-TOX-131, W2067 for further information.
Attachment B: MDL/LOQ Summary
ETS.8.960
ExtacoftPoFOnS fromUrine
001055 Page 11 of 14
Compound: PFOS
Prepared range of| LCR from curve| % Recovery
Human Urine| standards (ppb) (ppb).
Range
(ng/ml)
(ng/ml)
RSD
Range
CE EC I
`Compound: POAA
Psrteapnadraerddrsa(npgpeb)of| LCR frreom)curve| % RReacnogveery
fomtiemes[ | |n | |e w awae ea]]] (ng/ml)
(ng/ml).
qxua,dratic.
2.5--1p50p 0ppbb | 50ppb-- 1500ppb. 86-105
+-30%
Attachment B: MDL/LOQ Summary
ETS-8-96.0
001056 Page 120f 14
PArneaprydtaetes:
Ton Pair StaSntdaanrddaCradrnvuem-bUerri:ne Equipment number:
Sample mates: Methodirevision:
BFlinaanlksNoluviednitdaenndiTeNr:: Box Number:
TFaCrmgietxastndaalyptep: r05o00xpn.:
SFFuCCrrmmoiigxxatssetddstaadpppparrpoopssr..o$$.000010pp0oppmo:m:
SAcFctFouOanlSeconSdPceFeOoSmnAetroaPfSFtdStOciaSononAdeanArdss
iEnIhOe SKEC Scone
mixFORA Sdco
SEucGone
MSeEeWme|| AAmTt
FimAiWvol
1 gm gm wpm gel wpm sper | oem
60.350000 0500
[|03[0.0050010 [ |000 55000 |0 |T0005550 001011|||
00449%9 0499
||| 050505000]00[0| 5000.5500111||00 op0s0 i|06 2y02r--00775 s55|||
[[[53C00o00[ ]] s [[ssswo ww[sr ssooorrr |ag[vams
|
sso00o] seo |
[[0o0o0ts [--2z0o07m55| so |oms| zo
$00 [385000
[SI SS0010
500 |
500
sor |
S01 |
#95
499
S00 |
| S00 |
son
50.4
|
|
ooo |
0.0075 |
2007s
20100
S00[Sor[4s suo | sor |aon | ota
(CooT505001 | s1 or | #5500 Calculatedconceofnstatndarrdsantth siampolemnatsrix
sox oo|isorss]
FiPlRcOmoSnle C FiFnRalOcoSneAr PRFoiOnpSleeKolnAem FrEiWulOimoSnEe oFoFgaOlRm oAme FaWoSnc FnNiiSonm e a SSdecr hee aiAte
Tzes
mmer ew |= eis erat Sw Fina cone:
I CEeCSt SJ JCJ J3 1 +n |
oH S rwm eee me ts e a n ise e Ta Tnw eee]]
[TFCaOleSulte|d PcoRncOeSntrat_iPonFRsaOoflSseAtaAndaErdisOinSmEethanFForOlatRexaAtvreac (0F5Wrioslasmneea vowluemen)
:
ot
proll
Surg | piAnedtTmi]
E1C091E 00]B.
Ssooo
Soo Fa]
[sC5iTe5--0501T]--SFien|oe
E Eon oo O toute "iaw|
L s L m owe5
(1500
1503 |15|0 is0 s |
IEC IE
AvschmensC: StandardCaleulionSheetExEoTft SP-F6O9S6o f0omUn rine 001057 Page 30114
Prep Date(s): Analyst(s): Sample Matrix: Method/Revision: `Target Analyte(s):
Study Number: Equipment Number: Final Solvent & TN Number: Matrix Blank/Identifier: Box:
tt Toitial We/Vol. mL
(------
rr
--
--
rrr
1]
I
--
[1]
Er
Summaryof method: Notes:
`Atachment D: Weight'Volume Sheet ExacEoiTfSPo-O8n9S6f0om Une (014 058 Page 14of 14