Document XMq3aKepeLrqn8o494qMMNVg

(\Rm-ov\ ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE_________________________ Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA tetrabutylammonium salt, Ammonium perfluorooctanoate PFO, FC-116, FC-126, FC-169, FC-143 or as a major component of FC-1015 or FC-1015-X. (Octanoic acid, pentadecafluoro-, ammonium salt, CAS # 3825-26-1) Remarks: The test sample is FC-1015-X. It's purity was not sufficiently characterized, though current information indicates is a 30% straight carbon chain version of FC-143 in 80% water. The 3M product lot number was "HOGE 205". The following summary applies to the test sample as a mixture of the test substance in water solution with incompletely characterized concentrations of impurities. Data may not accurately relate toxicity of the test sample with that of the test substance. Data was used to compare toxicity of the branched/straight chain ammonium perfluorooctanoate homologue mixture in FC-143 vs. what is supposed to be the 100% straight carbon chain ammonium perfluorooctanoate in FC-1015-X. METHOD____________________________________________________ Method: OECD 209 Test type: Static acute GLP: No Year Completed: 1996 Analytical monitoring: Dissolved oxygen concentrations and pH. Statistical methods: Not applicable Test organism source: Activated sludge mixed liquor collected from the Metro Wastewater Treatment Plant, St. Paul, MN Test condition: Dilution water: Millipore Milli-QTM water Synthetic sewage: per OECD method Reference and test solution preparation: A stock solution of the reference substance, 3,5-dichlorophenol, was prepared by dissolving 500 mg in 10mL 1N NaOH, diluted to 30mL with Millipore Milli-QTM water, then brought to the point of incipient precipitation with 1N H2S 04 and diluted to 1L with Milli-QTM water. The pH of the reference solution was measured to be 7.2. Test substance was added directly to test vessels. Test vessels: Not stated 04040 Number of concentrations: 4 plus 1 reference substance and 2 blank controls Temperature: 19.1-22.1C Total suspended solids and pH on day of testing: 3.22 g/L (TSS in final 1.3 g/L) and initial pH 7.8 and final pH 7.9 Element Basis: Respiration inhibition as determined by oxygen consumption. Remarks: Values corrected to 20C for calculations RESULTS__________________________________________________ Nominal concentrations: 2 Blank controls, 420, 840,1660 and 3320 mg/L Element values: 30-minute EC50 = >3320 mg/L 3-hour EC50 = >3320 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture of the test substance as described in the test substance remarks field. The values reported apply to that mixture and not the test substance. CONCLUSIONS______________________________________________ The FC-1015-X 3-hour EC50 for activated sludge respiration inhibition was determined to be >3320 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY______________________________________________ Reliability: Klimisch ranking 2. Testing meets all criteria for quality testing, but lacks analytical confirmation of test substance concentrations. There is a lack of characterization of the test sample. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St Paul, MN, Lab Request N2169. OTHER____________________________________________ Last changed: 5/25/00 004041 ) / {1*'V ^'V%1- ~5 ^'>>Y-03'" ` - ''`-_i S > K *i ,: v` t>* %' ".v.u*Y.,; C O &042 mmmum ENVIRONMENTAL LABORATORY LR P l< o 3 ~Cr> OECO ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 TEST SOLUTIONS PREPARATION Test Substance: T^C- ~ IO \ 5 - ><____________________________________________ _ Sample Description: C lJccor* tc^c_itA______________________________ ;______ Analyst: ________ Date: - H - 3 5 - ? k A Test Cone. __mg/L__ mg added per 500 mL - - bi-t coOTeo l. -- M io S ID mL MQ(' ) water __per 500 mL__ 3 ZH mL syn. sewage (eed per 500 mL 1 <o mL microbial inoculum per 500 mL -r : Total Volume ... mL '2 .0 0 ______ S O o _ _ . aw / l> '2 C O 5co `-IT .O sew 1 to oo soo /tG -O ST3 o aw l(o T O O .. 5 oo 33 ao / (d 2 0 ( 9 S oo "3 VVtX icJ? p VV :*T Vt W - jA - t-K WisWtA ~ 1 T - -- asmiim amrnt, ENVIRONMENTAL. LABORATORY CR pg 3 OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST 209 RAW DATA _ X (, Test Substance: F C ~ /Q 15 Source of Activated Sludge Mixed Liquor: ro U - v W LiT* \V- r \ vr'l y Vl-4 P\c> w-f . ,) i Date Received: ~^ 5 ~c! Dat e Used: ~<3S ~ ^?Cc. Analyst: _ \*-5 m 30-MINUTE EXPOSURE DISSOLVED OXYGEN CONCENTRATIONS, mgfl. Test Substance TIME -MINU7 ES Temp . Concentration ( r ^ \u ) 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 C > BKCONTROLI H2c> 1 (obO 3 3 'A C . T .? '7.4, 1.7. L.-Y 0.5 t . o S.fc 7 4 '7. i (o.Y t 3 5 4 5. i V 4 6.t> S.1 5.3 4.0 4.4 4 1 S'.o 7.1 -7-6 1 3 1 .0 C*- & 6.4 73 4 ? 6.4 fc.a 5.sr 5.3 S it S3 4-< 3.1 6 .0 4l 4 .1 4T 3.5 5.1 4.4 y .t 4 .0 3-1 54 4 .1 4/ 35 1 .1 So 71 "". 2 0 .6 2t?.4 20 .y ^0 .7 52.1 ''V/ BK CONTROL# 7-1 to4 4.4 G o 5 -6 5 1 4-1 4.4 4.1 3 .7 3 i o ' F t A- 3-HOUR EXPOSURE DISSOLVED OXYGEN CONCENTRATIONS. \ mg/L ;# i ll Test Substance Concentration (n ^ lL ) 0.5 1.0 1.5. 2.0 TIME - MINL TES .' . Temp- 2.5 3.0 . 3.5 . .r4jo': J,-4'.5 ;s .o r - -&vr S l & f BKCONTROLI - -V 4 l O . F ~ /<o,T> 3330 7.? 1 7 7 .4 7 . 0 6 .3 (oM 6 .0 S lip ' G .'i 4 4 i f f l K c e r 7. r/4 - 6 .5 ' 1 S -T 5 -3 41-1 % '4.a 6 , 0 '5.4* 5 .0 M f(p 4 -3 'X 3 ; l' # 'S * ?*3.3- m .< & w cM k mm& m em H - c. a 5 : 9% :S5V6` 5 .3 5.0 31 3 i^ 1 .3 - 7 .4 T l `frJS? fewi- w & m m m w .41^ m m M i l I W i 8HM> ^"- -i' '.`If- sVY- *} ^ ,;: BKCONTROLI , IV 7.3. c% w m ' ^ i ?pH of highest concentration tested; *- > - i "XV 04044 {ENVIRONM ENTAL 1 W'-'J'- OECD ACTIVATED SLUDGE RESPIRATION INHI ON TEST ` 209 Lr. P1628 Date: 4 /2 5 /9 6 fc3Q-Mlnute ' Exposure MG/U* 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 4RIMRsnIm Z9BMSH& IM S m t BK1 " ' 7.8 7.8 7.2 ? 6.8 6.5 6.0 5.6 5.3 4.9 '4 2 0 7.4 7.1 6.8 6.3 5.9 5.4 5.1 4.8 4.5 ; 840 6.4 6.0 5.7 5.3 f 4.9 4.4 4.2 3.9 3.5 1660 8.0 7.8 7.6 7.3 7.0 6.6 6.4 6.0 5.7 3320 7.3 6.8 6.4 ' 6.2 5.8 5.3 5.0 4.7 4.4 S*?*'T- vVv." v*- cWxi'' BK2 * 7.1 6.8 6.4 1 *' 6.0 5.6 5.2 4.8 4.6 4.1 ft. d-idriin controls `Jr (4):-,!, ti v' EC5(H95% C l) . > 3 a o n ^ \ L . 5.0 4.6 4.0 3.1 5.4 4.1 3.7 5.5 T*mp. *C 4.1 20.6 3.6 20.9 2.8 20.8 5.0 20.7 3.8 22.1 R (1) 0.7721 0.7176 0.6167 0.6752 Ra (2) % Inh. (3) $ i W L 0.7348 0.7253 O 0.6788 6.1 0.5874 18.7 0.5834 19.3 3.4 20.9 0.7564 0.7105 RaCm (5): 0.7226 f i MG/L 0.5 1.0 1.5 S i W'WBWWjwawW BK1 - 7.9 ' 1 7.7 7.4 1420 '7 . 0 6.7 6.5 /8 4 0 6.0? 5.7 5.4 1660 6 .4 ' 6.2 5.9 # 3320 8 .7 * 8.5 8.3 2.0 7.0 6.1 5.0 5.6 8.1 2.5 3.0 mjwWmr&myner./ M 6.7 6.4 5.7 5.3 4.6 4.3 5.3 5.0 7.9 7.7 3.5 6.0 4.9 3.9 4.7 7.5 4.0 4.5 iti! 5.6 5.3 4.6 4.2 3.5 3.3 4.3 4.1 7.2 7.0 5.0 5.5 y V' *' 4.9 4.6 3.9 3.5 2.9 2.5 3.8 3.5 6.8 0.6 T*mp. *C 19.8 20.4 20.5 20.7 20.8 R (1) 0.6982 0.7297 0.7091 0.6036 0.4291 Ra (2) % Inh. (3) M P 0.7080 0.7067 o 0.6649 2.9 0.5750 18.5 0.4059 42.5 g-v^ * >-".r <" 'f-- BK2 v 8.8 8.6 8.2 T 7.9 7.8 7.2 6.8 8.6 6.3 5.9 5.6 19.1 0.6606 0.7033 Vi - Ec50(95% C l). > 3 a a o m o 11. giat j!'\ J RaCm (5): 0.7056 I ccnt/cf mmf t * wVtn 16% of oocfi offwr.) " ,-!-V .v'*v;'._ 'k \ 'il -i?. Br-f K S S O P Y AVAU ABU ENVIRNOMENTAL LABORATORY LR f l .T-U OECO ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 TEST SOLUTIONS PREPARATION Test Substance: 3 , S ~ O Sample Description: f t U r ' a W \ ~ V x O LftfeL lo-t- Q O ^ O K ? Analyst: * V- `^>__________ Oate: H - U S - 9 fe _____ Stock Solution Preparation: G o o ^ v i l L ; \ O w vl? r O =. n<n. 5 0 0 **M j,E. O c P M jJ n s ctA U d 4-p IO ^<3 1-OtJ M ^ o H O i\.J)\O vs u<Vi frv-vA*. i_0 A Vi ci/ifl'TOK *30Wll 1^ i i l -C? c o n i a r __________ A p P ro y 5? m l l-OSj U i.S ifit LA--s CXtid*?ci U-nci< n -r.V'r r i vnrj Ua V .\ 'im C .pl*vU PWc'.> ( .... 0 *\ ^ -V^JJ i . C U VO*A fYi \ \ i - Q u_'nJU* O' YS\ r-iCVe 1?\A ' 1 (r Test Cone. mL added mLMQH) water mL syn. sewage ma/L per 500 mL PSL5Q.Q SlL. feed per 500 ml :Tc<- IO |C iL l mL microbial Tolal Volume inasulmn-oec SOQ-mL, - mL OC* xt' o Soc ^3\A Q*T Wi^V>rs^ C-O^C . A<r-^~Vcc^ - -3 m-,^ '- ' v^V "`*v **' ' ' * ^ -1"! V (1) Milllpore Mdli-QR high purity water 5 W# ' <f'> 8 m ^" *' ".'-"",.-,-..::.-ftssvmsi *&$ LMkz' ' ^` >. r&?&m < .- ':V 1 ;,:- ..i's.'PAfrf ;>er^.'^KL'i* ^S'Vw-A'i, . .ifii wsiwnm ENVIRONMENTAL, LABORATORY IB-- ^ "CToS ? OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST 209 RAW DATA Test Substance: V . S - O ' i c . ^ o r o P ^ y ^ o l. Source of Activated Sludge M ixed Liquor: Date Received: ~ ^ ~r1^> A nalyst: " v 'O S ________ lO n-U c Date Used: P lo > 4 . S +P W -D . fVtO ^ ~ 3 S ? G, 30-MINUTE EXPOSURE Test Substance Concentration frrv^tO 0 .5 , ~ DISSOLVED OXYGEN CONCENTRATIONS, mg/L TIME -MINUT ES 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 Temp 5 .5 C BK CONTROL! IO 7 ? 7 0 7 . 2. G ? O S G o S' t S 3 + 9 T t t l a c 4, 7 9 7-9 7 fe 7 .4 97 fc.Y 4. L c 9 t-.a S.7 6 i . S i .0 BK CONTROLS "if:- y`'` i . i V 8 fc.M b O S.L, 5 . 7 H 9 `f v 4 | 3.7 3 4 a o .9 3-HOUR EXPOSURE "t f DISSOLVED OXYGEN CONCENTRATIONS', mgrt. l '. Test Substance TIME - M IN I TES '.: / Temp Concentration ( r ^ u - ) 0 .5 ; ViO',: 1.5 2 .0 2 .5 3 .0 3 .5 -*.;4.0' -,4.5 5 .0 5 .5 c >'-** ' BK CONTROL! . / r C-* 4 .9 * * * 7.<i . 7 . 0 4 4 G - t t '.O t i 4.9 4 .V m O '. . i "-frisar T V ` .'if 4 a, ? - 9 -0 %* .. & -T - m y - -S.H isrife ''ifa&Si*' mm sS fe .,;.*<(*> [ifrsh", M i * i tf. 1 a o _ ^ \- , *- ,-r - YSE?,7E>SKS- /M t*-'. &V3vV fife m m :#&** fltSStit' "iA*.'.* <d,, fi , ?*U AfiSS m m f^ :?S?i'ttJB 5*S6S;>. SjS W / w w - *-.- . 2 V' B K C O N T R O L r|^; if .'af ia '-.V' ; -7.9 >L f . 7 " -V f;-,-.-.^rx m ' ^ 9 5 . 4. 1 9 . i ' pH o f activated sludge mixed li q u o r : ; I n i t i a l * 7 . 3 ,2 - ( 5 *r.- pH o l highest r ^ f ;e n tr a llo n .tested: W -V .ln itla l ? 6> " ?* Test temperature:~JK S f # * g 3 _. w' wy V' v*5ft 004047 'V I ENVIRONM ENTAL LABORATORY; , VV O EC D ACTIVATED SLUDGE RESPIRATION INHI ON TEST .-209 ICALCULMAtTMIOMNteOieFiiRESULTS ^.4. Kst'" ^ " Test Sub8tance:|3.5^ICHLOROPHENOL,% ' . lAifflvstV-Trudv' Stiefenhoefer Ln P1628 Date: 4 / 2 5 / 9 6 Dissolved Oxygen Concentrations, mg/L MG/LSBXST SB i0.5? Wja'wp1KeamKM.vir0gy&psig?rimwa-^ # 1 .5 -' # 2 .0 2.5 " 3.0 3m Bsmi Km 3.5 4.0 4.5 5.0 MfcWKWS* 5.5 BK1' '7 .8 4 7.6 7 .2 & i '6 .8 - ' 6.5 6.0 5.6 5.3 4.9 4.6 4.1 ' ' `J':1 * 1 0 * 7 .9 ' 7.8 - 8fc7.65 7.4 7.1 ? 16.8 6.6 6.4 6.2 5.9 5.6 mm' 5* . jv.. i V;^;V,\, i flWiiMEl ffiefi**- KC.-i- u> ***: ri*? i ' ' y% -v$3WW' j V.-r~`c i dr -- -s - 3^%*- *<'. i:;.- = i i BK2 "7 .1 i 6.8 - 6.4 6.0 i 5.6 5.2 4.8 4.6 4.1 3.7 3.4 (% diff in controls (4): i 3.4 ; vr.-a'fflai sVitffl 3-Hour Exposure L > r Dissolved Oxvije n Concentrations, mi>/L I MG/L I 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 vtufjiik K awmSamwStmirw aEWwSw I w w If il: 4^ ' v '> 5.0 I 5.5 ' /V*<" f ip - BK1 7.9 7.7 7.4 7.0 6.7 6.4 6.0 5.6 5.3 4.9 4.6 l l l l 10 9.2 9.2 9.2 9.0 9.0 8.8 8.7 8.5 8.4 8.4 8.1 cv - - - -, * iss-:'1 U--..- 1 P II |1 1 BK2 - 8 .8 - '8.6 8.2 7.9 7.6 7.2 6.8 6.6 6.3 5.9 5.6 Tamp, *C R (1) - S U 2 L ^^eti^BlSI^H^BS^F'.plr*lF#i8WPll5f^. m&Sfiyjx*:. % Inh. (3) ;**^HHtUi 20.6 0.7661 0.7348 21.0 0.4848 0.4523 37.4 20.9 0.7564 0.7105 RaCm (5): 0.7226 Tamp, *C R (1) Ra (2) % Inh. (3) 19.8 20.3 0.6982 0.7080 0.2436 "02386 66.2 ! 19.1 0.6606 0.7033 RaCm (5): 0.7056 mnimmim - ; r *$8i.