Document XJbE28RxKjNwdZ5xp7RQDOpd
AR 226 _ 0877
BIODEGRADATION (OECD 301D)
IESTSUB~S~T~AN0G 000E 00 Identity: ArefmeirxrteudretocaosntPaiFnOiSng, pFeCr-f9l5u,oroorocatsanaescuolmfponoanteen,twhoficFhC-m7a8y08a.lso(1b-e
Octanesulfonic acid) (CAS # 2795-38-3). 7R8e0mBa.rkCsu:rrenTt hienf3orMmaptrioodnucitnidiocnatloets niutimsbaermiwxatusre2.ofT0h.e5t8e%stPsFaOmSp,le1i4s%FCfdioeatmheyrl,en1e.1gl5y%coslomdoinuombuocttyyll estuhlefart,e,760..78%%wpaotleyro,x5ye.t5h%yluerneea,mo1n.o1o7c%tyHlypdhreonxyyl ether, 0.05% sodium lauryl sulfate, and 0.05% benzotriazole. cThhaerafcotlelroiwiznegd csounmcmenatrryataipopnlsieosftiompaurmiitxiteusr.eDwaittha idnoceosmpnloettaeclcyurately reflect degradation potential of the fluorochemical component of the
test sample.
METHOD
000
M1e1t7h/o7d9:/GEORECD Closed Bottle Test, OECD Guideline 301D and C Test type: Ready Biodegradability GLP: No Year Completed: 1980 ASntaatliysttiiccaall mmoentihtoodrsi:ng:ResDuilstssolwveerdeodxeytgeernmidneepdlebtiyocnalculation of the % TBeOsDt/oCrOgDanoivsemr tsiomuerce: Non-pasturized field soil obtained from 3M Agricultural greenhouse, St. Paul, MN
Test coDnidliuttiioonn:water: Deionized water and distilled water.
-
pRreefpearreendceusainngd HtaescthsBolOuDtiSolnurprryepPailrlaotwiso.n:NitTrhifeicdaitliuotnioinnhwibaittoerr, wHaasch
aFtorthuemlrae2c5o3m3m,ewnadsedadcdonecdenttorraetpiloincoatfe0d.i1lugt/io3n0s0omfLb.lanks and samples
TNeusmtbveersoseflcso:ncNeonttrgaivteino.ns: 1 plus 2 reference substances, and
blank, all in duplicate.
TToetmaplersautsupreen:de2d0sColids and pH on dayoftesting: Not given. oe
Element Basis: Decrease in oxygen depletion compared to the
blanks.
002534
R0 ES 0000000
Nominal concentrations: Blank control, LAS and sodium dodecyl sulfate
(reference substances), and test material.
Element values: 5-Day BOD/COD: 15-Day BOD/COD:
21% 78%
28-Day BOD/COD: 72%
SRuebmsatraknsc:e TReesmtianrgkwsafsielcdo. nTduhcetveadluoenstrheepmoirxtteudraepapslydetosctrhiatbemdixitnutrheeaTnedstnot the fluorochemical proportion alone.
c0 onclusion0 s 000 wTihteh tneistrtifsiucbatsitonanicnehib%itBedOwDa/sC8O3Dawftaesr 2782 daaftyesr.28days. The % BOD/COD
Submitter: 3M Paul, Minnesota,
Company, 55133
Environmental
Laboratory,
P.O.
Box
33331,
St.
pa0 raQ0 uan0 ry 000000000
mReeltiahboilditfyo:llKolwiemdi.scThhreaniknioncgul=u3m.isSntoutdyprloapcekrslydecshcarriapcttieornizaendd. rTehcoerdssamopflthee. cpuorniftiyrmwaatsionnootfptrhoepearmlyoucnhtaroafctfelruiozreodchaenmdictahlepsrtoupdoyrtliaocnksinatnhaelystoilcuatlion.
R0 EFERE0 NCES00000000
TPahuel,stMuNd,y wLaabs Rceoqnuduecsttendubmybethre632M11CSo,m1p9a8n1y., Environmental Laboratory, St.
O0 THER 000000
Last changed: 6/28/00
.
002535
PF
: refers
Test Guideline C 117/79/63R
Test Guideline for the Closed Bottle Test acc. to Fischer Date of last revision: August 27, 1979 Level I test for ready biodegradability
1. 2$4 ~>
Prercauisites Iint htahse tcoonbceentkrnaotwinonwhertahngeer etmhpelotyesetd mina.tetrhioaltesits s(2olu-bl1e0 mogf/1s)o.lubIifliiztinigs anogte,ntssp,cciualltramseoausnudresdissupcehrsiasontehetcamdadyition have to be applicd.
2.
. s+
32" 77.
A pwv r
f 3.
,\@p JY wo
A W471oT
WW [Ne
Guidance Information Ktniocswleodfgetheof ttohset mbaatcetreirailal istoxniotcituyneqoruivionchailbliytorryequpirroepder
but constitutes u:seful information.
Qualifving Statements Tofhe ortgesatnicis coampppoluincdasblebutfornotthefobrimoidxecgurracdsabitlhierteyofe.valNeusattion experience has been gathered with water soluble compounds, haloswoevetro', thtehebitoesdtcsrlaednadsbiliittseylfe,vaaltuatlieoasntofinvoplraitnciilpelea,hd
insoluble. compounds.
v
002536
Loy 5
August 27, 1979
LRtbuls
,
OECD
Test
Chemicals
Guideline
Testing
for the
Programme
Closed Bottle
Test acc. to Fischer
0:
The test P.Gerike,
parnodceRd.urSechinsidbasiendWaosnsear-Papuenrd.
by WK Fischer, Abwasserfors
2, 99 -118 (1974),
chung
1,
Method
+1 Itdnotnrodoufcttieosnt:anPdurepxospel,anastcioopne,ofrelleimviatnsc.e, and applica-
ui' - EN
aTbqhiueoedoepugusrrapmdoeasdbeiiulomiftaytthoeafmeotetrsghtao'ndiccoinscceotnmhtperoumnedassurienmaenntaeorfobtihce,
47
concentration) data elav
to
10
mg/l
of
ation of 2 (standard
active material, Most
:
Sompounds,orahotweedvewri,thvotlhaitsilteestcomppeorutnadisn
low water solubility
to water-soluble
and those of
be tested.
: may alse, at least in principle,
1.2 1.2.1
DDefeinfitionns iaanntdouunnniittss
v
DefinitiFonrooofr bio2 demggra0d,abi/imigtyAS
ror
% Bop/cop: --T mg 20E , /Nmg S AS mg COD/mg AS
ve off
we eA KL
&
vw
A
& we
!
002537
mires on
for
J Bai
Ge
Fie: PaaGe2UnTe
mE
"7%. ENVIRONMENTAL ENGR.LAB --WORK SHEET
SAMPLE DESCRIPTION ; Fo
i
--
Clad Pte Tut
owviev:re:_LLI0a=d3e(@=. 8S0idi Woe
ti The dbZirn win Coninanald nhierd oolTio) wrne
paps sist MACH 00DSiig Pillove
2. NiuffoiaTivtn inden -- HACH FibmuiA 233, cmos
at He decom toneeTonTini of 0.19[So0ml.
[Er --
oo
002538
i
Form ShIeHetEIfoRrEthNeTCAloLseLdABbCoRtAtTlEeRYTes|t
Ref.: OEC1D1,7T/o79k/yGoE,R 12-01-7
Lb request no. 6211s
5 * LAT
Gace of stoaftrasets_10-31-80 LCT Se AuScheo ll
Tose 7 seandard macarior:_A=EFE
Theoretical tase conc. 197: ra
3007 of test matartal:
ma a/mg ns coo of test merertets__ 0.3% mous aa
ini)
aVShrIiL R areA aRIT ERETSeat a ses ween H Temp. of the dilution water after aeration:
293 x
20 %
-- [omemow To [me TP o 90) ow
Gm,
=
EEA
Ti
wrmr
To.
[FE Tonlunlu [ow| ih aTenanes |on| :
[Co [ax [oooifa8.r75o]bs8.o55r| 86..8a5sw | wioorluetsae theme
[CsTelenas Tem Totton
[ [& wo e TToo5n[o8T f.e:7s0i[e8s5o5r[| s7.a10o | Ccmoaintetiraoiinfni
Tammese | 22 =,
, [4.10[7.c0(9,5 IN "2g bY
x
I [9.048.504 S120 *|4
wseheoven.at
8: 0pOELETIONS(mg 80071) ar AFXTOEAYRS: minis wage Win} + nde
a
aitator OLAS [oT Iheqaiiriedforms is,te paenired latsial 6 con. of
A1
o ties. sen Cw
ee--tatweted =AS +hoor Ti
% boGolJiena?n WRFatin
mma oe ]
AERA EL
[- Loerie|
(|wsieaissi]l
The ample so tonsicrid 2 be 1007s aituse (ing burp = Img 45).
EEE Re
002539
.
--
Ref.: OECD, Tokyo, 12-01-7
Form Sheat for the Closed bottle Test|
17/79/67
Lab request no. 6211s
Date ofscareof tascs_10=31-80
sea A,t Schs ell;
Test / standardmaterial;DODECYL SueHATE, So01UM SMT hacraticol tast conc.
2.0 wasn
B00T of test materials,
29 0/mg AS C00 of test materials 2:0 wg gu wa TRIW Suara)
HI SSRI bes man Tews. of the dilution water after serationsx____(_2209%3)
+ som; Inoculum: unfertilized garden soll
[omen [ope [we ToPRT0
huEltahout EInoc,ul| um. e [D ee n es 2 5== Lke n = ax [oebaiofasor| sass| wanes
[ms[denon |ew | ft mmm
EEE
TnociTem.
a
[m[olT e er ser| HTaa
[no w lees so| ese | vSahluveeesd;,
Tw Tooleofaso | esa | therde
C [ & I T T15o.1i5]8a.a7s0t]a8.s5o5r| 7[a.o1r 0|| caiocnrtiafihn
| m= [reeTTeeolnefnosn|founn| Wik
1[29..00054[6e..3300[7S740 5%||_34..560 0%| *see not
11
shove.
ATI
ait 1,5 rn} + lh -
TRG STENT TB SL RO ry ee
LE0T 0
a xm mam ow
ng AS/1 + a00T
=:
, Fe--torrbd he --
UBufers TFFodie hh Fed
3
:
002540
.
In umes Dsouraar
Ref. ECO, Tokyo, 12-017
:
Form Sheet for the Closed batcle Test|
117/79/GeR
Lab request no... 6211s
Date of start of tese;_10-31-80
anatyses_ Wo A, Sche
Test / standard material; LAS REFEREALE SAMPLE tracratical test conc.
2:52 ues
007 of tone metertats__ 2.24 Poe riog a5 ct of tort ewtariats_2-4S PIE
di
Teme. of the dilution water after seration:_
293 x
20 %)
HisLsnE OyeayT e SyAissTna
a ale ete
ee he ilsnet
tr
*
--
wm | | [sdseem|ow] Ae,
[Is 85|e 720|
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ax
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[670.0|| sow |
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=
ati
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irociim.
LTR il [| 9.s[09].780e[8s.]6c570o0s. [[43.750 | * [9,058,604 6.50 *| 4, FS *| *see note
[= Jam[eefent gn | 9 OEPL(mEg 00T/1)IAFToONXNOMSY:
800 = (ny =m) = (mg mm)
MID FOAL My= (mm) (mem)
-
BL LTTE BSL me eee
tl
a mg + mum ow
Tw m5 AS/T + Boor [7i[#5 [h4]9|
v-
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7 Bo0/can Rircetin (nhibFid 5)
0.0:
-
1.
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wry cibetaticl
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2. tAs Refrmee Soph, # (2 7)8) Date 115, avy Mu 318,
ASnSCPSeTacPtiAie Laasr; ePaHsepaSroeadp.sboydmoDgeatntLi,gvoenrteB~oCsroeneenutnimvan.tu(55528 4
TT 3M
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ANALYSIS FORM
Ll Aeee-a
favhementel fatratony |ne RFS
aia
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v
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[arated Bilin far| | TT 1TT
bl
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ls
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Yoabaa)
----
BEST COPY AVAILABLE vo re B2ULS
ENVIRONMENTAL ENGR. LAB --WORK SHEET
SAMPLE DESCRIPTION Chsd Att / Tor+ StatpotJo3i-fo
Sxtra Blobs
Envtvimmental Lae DI tio [NADishlled) sonek
Yiliznte ancl wnZh insect.
TlH
Da,
Mn,
On :
Te, 28
24
2/10
8.6 - gh
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8.5%
6,88
74
9.20
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6.8%
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9.20
8.8
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7.70
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BESTCOPY AVAILABLE
*
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ENVIRONMENTAL ENGR. LAB -- WORK SHEET
SAMPLE DESCRIPTION
Date: (0-31-80
Prspasitiris of Stroh debtioria) wasel fn
y the "thos Bittle Toot' = oecp Ts i 12-01-79, 117/79 JakGer
_--
Regret ds 20, 2
2 7 22 [00208 san. a
24
;
Serpe DiseniaZios oe Anand _damgat eae mg COD pen
AFF a REEF - B
\
Sy |
Jy
AEEE-C
147 | oma |
BT hoo| ge |
LAS Kup Pi
51
Go 6.0
0
40
6.2 0g (2.5ng #
-_--
~
paspancd 10-31-80 b 0.854
vr
Date of Shart of Tee--d jo-7)-80
-_-- -_--
(2) Detieyl autiat, poslivms oucts, (purlians Latrgl --_-- rot #BE mwasses Cy (Hs), 0507 Na
putfet), leosih tos. 5.
@ hs REFEREE purple, LTTM (7/381DES, gy Iw318_Qetive LAS 5
Puiporsel by PIDORRL NERC- Cdimsed U2Ps AGE hose, oC
OoP Lioe nuird HeSop OBAGAA Bose.
002544"