Document X8zY199a9qVbjn571zwmLxrKG

A R & & 6 - OZZS ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE___________________________________________ _ Identity: Mixture containing perfluorooctanesulfonate which may also be referred to as PFOS, FC-95, or as a component of FC-3003. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not given. The test sample is FC-3003, identified by the laboratory as R1331. Current information indicates it is a mixture of 1% PFOS, 15% diethylene glycol butyl ether, 79.39% water, 2% Sultone foamer, 1.5% sodium octyl sulfate, 1% sodium decyl sulfate, 0.06% sodium lauryl sulfate, and 0.05% benzotriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect toxicity of the fluorochemical component o f the test sample. METHOD:____________________________________________________ Method: OECD Guideline 203. Type: Acute static GLP: No Year completed: 1997 Species: Pimephales promelas Supplier: In-house cultures maintained at AScl-DETD, Duluth, MN Analytical monitoring: Temperature, pH, conductivity, and dissolved oxygen. Exposure period: 96-hours Statistical methods: The LC5ovalues were calculated by Trimmed Spearman-Karber method. The NOEC values were calculated by Fisher's Exact test using Toxstat statistical software, Version 3.5. Test fish age: Juveniles Length and weight: Average length = 16.6 mm Average weight = 0.036 g (wet) Loading: 0.12 g/L Pretreatment: None Test conditions: Dilution water: Dechlorinated City of Duluth, MN tap water, post carbon filter. The water was aerated for 24 hours prior to the test. Dilution water chemistry: Hardness: 48 mg/L as CaCC>3 Alkalinity: 44 mg/L as CaCC>3 Conductivity: 119 pmhos/cm pH: 8.08 Lighting: Cool-white fluorescent lights. A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period. 002736 Stock and test solution preparation: The test material was added directly to the dilution water on a weight/volume basis. Concentrations dosing rate: Once. Stability of the test chemical solutions: Not noted. Exposure vessels: 4 L glass jars containing 3 L test solutions. Number of replicates: 2 Number of fish per replicate: 10. Number of concentrations: five plus a blank control Water chemistry during the study: pH range (0-96 hours): 7.68 - 8.08 (control exposure) 7.60 - 7.92 (1000 mg/L exposure) Temperature range (0-96 hours): 20.5 - 20.7 C Conductivity range (0-96 hours): 119-121 pmhos/cm (control exposure) 1 3 3 -1 3 6 pmhos/cm (1000 mg/L exposure) Dissolved oxygen range (0-96 hours): 6.9 - 8.7 mg/L (control exposure) 5.1 - 8.6 mg/L (1000 mg/L exposure) RESULTS____________________________________________________ Nominal concentrations: Blank control, 130, 216, 360, 600, and 1000 mg/L Element values: 96-hour LC5o = 472 (376 - 593) mg/L 96-hour NOEC = 216 mg/L Element values based on nominal concentrations Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ The test sample 96-hour LC50for fathead minnow was determined to be 472 mg/L with a 95% confidence interval of 376 to 593 mg/L. The 96-hour No Observed Effect Concentration (NOEC) is 216 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. * Paul, Minnesota, 55133 DATA QUALITY________________________ :______________________ Reliability: Klimisch ranking = 2. Testing meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. 002737 REFERENCES This test was conducted by AScI Corporation, Duluth Environmental Testing Division, Duluth, MN at the request of the 3M Company, Lab Request number R1331, 1997. OTHER______________________________________________________ Last changed: 6/26/00 002738 AScI Corporation/AScI-Duluth Environmental T oting Division AScI Study ID# 5010-068 ST.UPY.TITLE ACUTE TOXICITY OF R1331 TO FATHEAD MINNOW (Pimephales promelas) BAXAJS1AMPARP OECD GUIDELINE 203 S.TU.DY1.CQMPLETEP June 26, 1997 TESTINGFACILITY AScI Corporation/AScI-Duluth Environmental Testing Division 4444 Airpark Boulevard Duluth, MN 55811 TeL No. (218) 722-4040 Fax No. (218) 722-2592 S H IP Y..IPNT1FIGATIQN -NUMBERS AScI Study ID# 5010-068 3M Company Lab Request# R1331 or Sponsor 3M Company Sponsor Study ID# R1331 1 002739 1.0 GENERAL INFORMATION AScI Coiporation/AScI'Duluth Environmental Testing Division AScl Study ID# 5010-068 Study Title Data Standard Sponsor Sponsor's Representative Sponsor's Lab Request# Testing Facility I Principal Investigator Project Director Testing Facility Director Definitive Test Dates Data Validation and Report Review Acute Toxicity of R1331 to Fathead Minnow (Pimephales promelas). OECD Guideline 203. 3M Environmental Technology and Safety Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133; Tel No. (612) 778-7452. Susan Beach, 3M Environmental Technology and Safety Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133; Tel No. (612) 778-7452. '* R1331. AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. ` Joe Dierkes Joe Amato Donald Mount June 17-21, 1997. T* Alan Mozl Report Signature; ~ Retention of Raw Data and Final Report Location of Raw Data and Final Report Two years from the date study is completed. , %| AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. Sponsor: 3M Company Sponsor Study ID# R1331 2 00^740 2.0 OBJECTIVE(S) AScI Corporation/AScI-DuLuth EnvironmenUl Testing Division AScI Study ID# 5010-068 To determine the 96-hour 10% and 50% effect concentrations (LC10 and LC50), and the 96-hour no observable effect concentration (NOEC) of the test substance, R1331, for fathead minnow .. (Pimephales promelas) under static test conditions. This study was conducted according to the AScI study plan retained by the Sponsor and OECD Guideline 203 (OECD 1993). 3.0 TEST METHODS Test Substance Properties: R1331, amber liquid, specific gravity ca. 1.0 (water = 1), stable, and completely soluble in water. Test Organisms Dilution Water Test concentrations'. 0 (dilution water control), 130, 216, 360, 600, and 1000 mg/L. Test solutions prepared by mass addition of test substance tq dilution water followed by vigorous stirring. Source: Healthy, juvenile fathead minnows {Pimephales promelas) obtained from AScI-DETD in-house culture. j. Holding/acclimation: The fish used in the tests were obtained from AScI's u in-house culture. The fish used in the study were hatched between May 17 1 and 23, 1997. For the 48 hours preceding test initiation mortality was 0%, 1 and the fish were not fed for 24 hours before test initiation. The fish mean I length was 16.6 2.5 mm, and mean wet weight was 36 19 mg. | Loading rate was calculated to be 120 mg of fish per L test solution. f Use in test: Used 10 organ isms/replicate and 2 replicates/test concentration. Source: Dechlorinated City of Duluth, MN, tap water. The most recent chemical analysis is in Raw Data Package. q Characteristics: At test time, the water had a hardness of 48 mg/L (as " j CaC03) and a pH of 8.08. Aerated for 24 h prior to use in the test. | )1 Sponsor; 3M Company Sponsor Study ID# R1331 3 002741 AScI Corporation/AScl-Duluth Environmental Testing Division AScI Study ID# 5010-068 Exposure Chambers Incubation Type: 4-L glass jars. Use in test: 3.0 L of dilution water or test solution/chamber. During the test, kept covered, except when experimental observations were made. Solutions were not aerated during the exposures. Duration: 96 hours. f Daily photoperiod: 16 h light and 8 h dark, maintained using cool white fluorescent lamps. i Observations Temperature: 21.0 1C. Biological: Daily- Test organisms with abnormal behavior and signs or stress, and dead test organisms. < Endpoint Calculations Water chemistry: (1) At test initiation- total hardness and alkalinity, (2) daily- temperature, DO, conductivity, and pH. All determinations were made according to APHA methods (1995). LC10: Inhibition Concentration (ICp) Method. a 1 I | 1 LCS0's: Trimmed Spearman-Karber Method. NOEC: Fisher's Exact Test (TOXSTAT statistical software, Version 3.5, . . . . . West Inc,, and University of Wyoming). Sponsor: 3M Company Sponsor Study ID# R1331 4 002742 AScI Corporation/AScl-Duluth Environmental Teiting Division ASct Study ID# 5010-068 3.0 RESULTS 1........ .. ' ' l,"i " ....... Test Substance Toxicity (Pertinent data are in Table 1) 24-h LCS0: > 1000 mg/L (Not Calculable). 48~h LCS0: > 1000 mg/L (Not Calculable). 72-h LCS0: > 1000 mg/L (Not Calculable). 96-h LCjq. 173 mg/L (Not Calculable). 96-h LCS0: "472 mg/L (376-593). Ranges of Water Chemistry Parameters 96-hNOEC: 216 mg/L. Total hardness (as CaC03 mg/L): 44-48. Alkalinity (as CaC03 mg/L): 42-44. Temperature (C): 20.5-20.7. DO (mg/L): 5.1-8.7. pH: 7.60-8.08. Specific conductivity (umhos/cm): 118-136. I 1 | Sponsor. 3M Company Sponsor Study ID# R1331 5 002743 4.0 TEST QA/QC AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 | QA/QC Criteria Data 1 Mortality in control exposure must not be more 0% mortality. | than 10%. [ The lowest DO concentration at test | termination must be at least 60% of the air saturation value at test temperature. The lowest DO concentration measured was 57% of the air saturation value at 21.0C. The lowest value was measured at the 72-hour test interval for the 1000 mg/L concentration. At the 96-hour interval the DO was 64% of saturation. The reduced DO at the high test,, concentration did not appear to invalidate the test as significant lethality and acceptable DO's occurred at the 600 mg/L test concentration. Test duration must be 96 h. The test duration was 96 h. | Reference Substance Toxicity Test (this test is Control chart for sodium chloride reference * j | optional). toxicity testing is in Raw Data Package. | 'ir iv Sponsor 3M Company Sponsor Study ID# R1331 6 0G&^44 AScI Corponition/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 5.0 REFERENCES IAmerican Public Health Association (APHA). 1995. Standard Methods for the Examination of . Water and Wastewater. APHA, Washington, D.C. Organization for Economic Cooperation and Development (OECD). 1981/1993. OECD Guidelines for Testing of Chemicals. OECD Publication Information Center, Washington, D.C. Hamilton, M .A., R.C. Russo, and R.V. Thurston. 1977. Trimmed Spearman-Karber Method for Estimating Median Lethal Concentrations in Toxicity Bioassays. Environ. Sci. Technol. 11(7): i 714-719; Correction 12 (4): 417 (1978). - USEPA. 1993. A Linear Interpolation Method for Sublethal Toxicity: The Inhibition WCoenscteInntcra.,tioannd(IUCnpi)veArspiptyrooacfhW. yVoemrsiinogn. 2T.0o.xstat Software Package. Version 3.5. Cheyenne, Wyoming. w vJ; p Sponsor: 3M Company Sponsor Study ID# R1331 7 002745 AScl Corporation/AScI-Duluth Environmental Testing Division AScl Study ID* 5010-068 TABLE 1. ACUTE TOXICITY OF R1331 TO FATHEAD MINNOWS (P. promelas) I Test Substance I Concentration (mg/L) 1 0 (control) 1 130 j 216 360 600 1000 Cumulative Number of Dead Organisms and Percept Mortality* . 24 h 48 h ' 72 h ; 96b 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 0 (0 ) 0(0) 1 (5) 4(20) . 0(0) 0(0) 1(5) 9(45) 0(0) 0(0) K5) 9(45) 3(15) 3(15) 9(45) 18 (90) H - Percentages are in parenthesis. LETHAL AND NO-EFFECT CONCENTRATIONS WITH CORRESPONDING 95% CONFIDENCE INTERVALS .. LC's and NOEC 24-h LCS0 48-h LC50 72-h LCS0 96-H LC10 96-h LCjo 96-h NOEC ' Values based on nominal test concentrations. Data (mg/L)* >1000 >1000 >1000 173 472 216 95% Confidence Interval - { | Not calculable Not calculable Not calculable Not reliable 376-593 Not applicable Sponsor: 3M Company Sponsor Study ID# R1331 8 002746