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ACUTE TOXICITY TO BLUEGILL SUNFISH TEST SUBSTANCE Identity: Perfluorooctanesulfonate; may also be referred to as PFOS or FC-95. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The test substance is a white powder (3M Lot 583) of uncharacterized purity. METHOD Method: Not provided Type: Static acute GLP: No Year completed: 1978 Species: Lepomis macrochirus Supplier: Not provided Analytical monitoring: pH, DO and temperature Exposure period: 96-hours Statistical methods: Not provided Test fish age: Not provided Length and weight Average length = 3.0 cm. Average weight = 1.2 g Loading: 1.5 g fish / L Pretreatment: None noted Test conditions: Dilution water: Laboratorywell water Dilution water chemistry: Dissolved Oxygen: 9.9 mg/L pH: 7.6 Stock and test solution preparation: Not provided Concentrations dosing rate: Once Stability of the test chemical solutions: Not noted Exposure vessels: Glass aquada containing20L of test solution. Number of replicates: one Number offish per replicate: 20 Number of concentrations:five plus a negative control Water chemistry during the study: Dissolved oxygen range (0- 96 hours): 5.7 - 9.9 mg/L (controlexposure) 4.8 - 9.9 mg/L (100 mg/L exposure) pH range (0- 96 hours) 7.6 - 7.8 (controlexposure) 7.6 - 7.7 (100 mg/L exposure) Test temperature: 19 - 20C