Document X87nVpaBzD4JG5vwvOZ2kQ3Dw
AR 226 - 07/9
ACUTE TOXICITY TO AQUATIC INVERTEBRATES (SCUD)
JESTSUBSTANCE
Identity: A mixture containing perfluorooctanesulfonate, which may also be
referred to as PFOS, FC-95, or as a component of FC-203. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was not noted. The test sample
is FC-203. Current information indicates it is a mixture of 1.34% PFOS, 35%
diethylene glycol butyl ether, 37.85% water, 20% ethylene glycol, 2.66 %
Sultone foamer, 3% sodium octyl sulfate, 0.1% sodium lauryl sulfate, and 0.05% tolyltriazole.
The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. Me ETe HODeeem------------------------
Method: Standard Methods (APHA), 1970 Edition
Type: Acute static
GLP: No
Year completed: 1973
Species: Gammarus fasciatus
Supplier: In-house cultures maintained at Bionomics, Inc., Wareham, MA.
Analytical monitoring: Temperature, pH, conductivity and DO
Exposure period: 48-hours
Test organism age: 12-24 hours old (fourth instar) Statistical method: TLso (median tolerance limit) values calculated using a
linear regression equation.
Test conditions:
Dilution water: Well water
Dilution water chemistry:
Alkalinity:
35 mg/L as CaCO3
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pH:
73
Lighting: Not given
Stock and test solution preparation: Exposure concentrations prepared by
direct addition of test substance.
Exposure vessels: 250 mL beakers with 200 mL exposure solution
Number of replicates: 4
Num of ob rgaenisrmsperreplicate: 5
_
Numberof concentrations: five plus a blank control
`Water chemistry during the study:
PH range (0-48 hours): 7.1
Temperature range (0-48 hours): 211 1C
Dissolved oxygen range (0-48 hours): 5.3 -- 8.9 mg/L.
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R0 ESw0 YS00000000000 Nominal concentrations: Bk control, 560, 870, 1000, 1800, and 3200 mg/L. Element values: 48-hour ECso = 1130 (843 - 1310) mg/L
Element values based on nominal concentrations
Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
Conclusions
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The test substance 48-hour ECs; was determined to be 1130 mg/L with a 95% Confidence Interval of 843 to 1310 mg/L.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
p0 ar0 agu0 ay0000000000 Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution.
R0 EFER0 ENC0 ES 0000000000 Test was conducted by Bionomics, Inc., of Wareham, MA at the request of the 3M Company, St. Paul, MN, 1973.
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BIOASSAY REPORT
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SUBMITTED TO
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ST. PAUL, MINNESOTA
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ACUTE TOXICITY OF 3M SAMPLES (A AND B) TO THE WATER FLEA
(Daphnia magna)AND SCUD (Gammarus fasciatus).
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METHODS AND MATERIALS
These investigations were performed at the aquatic
invertebrate toxicology laboratory of Bionomics, Inc., Wareham, Massachusetts. The susceptibility of the
water flea, Daphnia magna and scud Gammarus fasciatus
0 3M samples designated as sample numbers A and B
(100% active) was evaluated under static conditions for
a 48 hour experimental period. Results were. expressed
as the median tolerance eteTg Bh nominal concentration of the test compoundin watercausing
nds
50 percent mortality. TheTLsg values and 95% confidence
intervals were calculated by converting the test
concentration and the corresponding observed percent
mortalities to logs and probits, respectively. These
values were then used to calculate a linear regression
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equation.
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Both Daphnia and Gammarus were obtained from laboratory cultures and judged to be in excellent condition. Procedures _
for the static bioassays adhered to the protocol described
by the 1970 edition of Standard Methods (APHA). Static Daphnia and Gammarus bioassays were conducted in 250 ml beakers with 200 ml of the experimental diluent. The
diluent water was obtained from a well source and was used as such. The experimental chambers were maintained in a
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water bath at 21 1C. Five 12 * 12 hour old Daphnia
were introduced into each experimental beaker and five fourth instar Gammarids were introduced into each experimental chamber for the Daphnia and Gammarus bioassays, respectively.
In the Daphnia bioassays, food was added at a rate of
0.5 ml/liter after the introduction of the compound to
the test vessels.
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Bioassays were conducted using four replicates of each
concentration for both compounds (A and B) for both species.
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The pH of the standard diluent was 7.1 and the methyl orange
alkalinity was 35 ppm as CaCo,. Dissolved oxygen values
for the various test vessels for both compounds ranged
from 8.9 initially to 5.3 mg/l at the end of the test.
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Food was prepared by homogenizing 5 g of starter trout
food and 1 g of Cero-phyl in 100 ml of water.
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RESULTS
The predicted 48 hour TLg, (i.e. that concentration of
active compound that allows 50 percent survival to the
experimental animals) and 95% confidence intervals for
Gammarus and Daphnia exposed to compounds A and B are presented
in Table 1. A summary of the observed mortality of
Daphnia and Gammarus exposed to various concentrations of
compound A is given in Table 2. The observed mortality of
Daphnia and Gammarus exposed to experimental concentrations
of compound B is given in Table 3.
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SUBMITTED BY:
PREPARED BY:
APPROVED BY:
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Bionomics, Inc.
790 Main Street
Wareham, Mass.
December, 1973
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S. Krogh Derr, Ph.D.
SX xeooy Nese
Invertebrate Toxicologist
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Kenneth J. Macek, Ph.D.
Director
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Table 1 - Acute toxicity of 3 M compounds A and B to the water
flea (Daphnia magna) and scud (Gammarus fasciatus)
exposed for a 48 hour experimental period.
Species
48 hour TLgg-mg/liter
CompAonug,nd CompBou 55;nd
wet Me
Daphnia`magna
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31.1 (23.3241.5)
GammaSruusdfasciatus 15.4 (12,8-20.1)
1560.0 (1280.0-2140.0
\ 1130.0 (843.0-1310.0)
)
Bioassay conducted at 21 1c, Daphnia, 12 12 hours old
at initiation of tests.
PBioassaysiCeonducted at 21C * 1c, : Gammarus, fourth gear at a
initiation of tests.
a
95% confidence intervals.
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Table 2 - Concentrations tested and corresponding observed
percent mortalities for the water flea, (Daphnia
magna), and such (Gammarus fasciatus) exposed to
3M compound A for a 48 hour experimental period.
Species
_--
Concemngt/r1ation
% Mort4a8lihtoyurObserved
Water Flea
42.0
.
100
(Daphnia magna)
32.0
60
2.0
0
t
12.0
0
10.0
0
)
7.5
0
+ Control
Scud
32.0
(Gammarus fasciatus) 24.0
18.0
12.0
7.5
Control -_--
0
"
00 70
. 60 50
0
0
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Table 3 - Concentrations tested and corresponding observed
percent mortalities for the water flea (Daphnia magna)
and scud' (Gammarus fasciatus) exposed to 3M compound
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B for a 48 hour experimental period.
Species
Water flea
(Daphnia magna)
Concemngt/ra1tion
3200.0
1800.0
1000.0
870.0
560.0 Control
7 Mortalit48y hOobusrerved
100
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45
5
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Scud
3200.0
(Gammarus fasciatus)
.
1800.0
1000.0
870 .0 560 .0
Control
--_--
100
90
w
4st
40
0
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