Document X7p48dqb14K6xgB5Dvry8R4qR

ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity:A mixturecontainingperfluorooetanesulfbnatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component ofFX-3104. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was not noted.The testsample isFX-3104,6%. Currentinformatioinndicatesitisa mixtureof0.48% PFOS, 14.09% diethyleneglycolbutylether,59% water,17.6% ethyleneglycol, 1.44% sodium octylsulfate4,% inhibito2r.,0% polyoxyethylene monooctylphenylether,1.25% propane sultonefoamer,0.03% sodium lauryl sulfatea,nd 0.1% benzotriazole. The followingsummary applies toa mixture with incompletely charactedzed concentrations of Impurities.Data may not accurately reflectthe toxicityof the tluorochemical component of the testsample. METHOD: Method: Not given. Type: Staticacute GLP: No Year completed: 1985 Species: Daphnia magna Supplier:In-houseculturesO.riginalloybtainedfrom U.S.EPA-ERL, Duluth, MN. Analyticalmonitoring: Temperature, pH, and dissolvedoxygen. Exposure period: 48-hours Test organism age: <24 hours old Statisticalmethods: EC5o values calculatedusing logistircegression methods (probiatnalysis). Test conditions: Dilutionwater: Carbon-filterweedllwater. Dilutionwater chemistry: Not given. Lighting: Not given. Stock and testsolutionpreparation:A primarystocksolutiownas prepared by dissolving1000 mg oftestsubstance in500 mL water. Exposure concentrationswere preparedby additionofstocksolutiontoeach vessel. Exposure vessels: 250 mL glassbeakers containing200 mL exposure solutionata depth of6 cm. Number of replicates:2 Number of daphnids per replicate:10 Number of concentrations: fiveplusa blankcontrol Water chemistry during the study: pH range(0-48hours): 8.1- 8.2(contreoxlposure) 7.7- 8.1(1000 mg/L exposure) Temperature range (0-48hours): 22 OC Dissolved oxygen range (0-48hours): 8.9- 9.3 mg/L (controelxposure) 7.5- 9.3 mg/L (1000 mg/L exposure) RESULTS Nominal concentrations:Blankcontrol1,00, 180,320, 560,and 1000 mg/L. Elementvalues: 48-hourEC5o=>1000mg/L Element valuesbased on nominal concentrations. Remarks: Testingwas conducted on a mixtureas describedinthe Test Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsubstance 48-hour EC50 was determined tobe >1000 mg/L. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 3. Study lacksdescriptioannd recordsof method followed.The studylacksinformationon purityofthetestsubstance and analyticaclonfirmationofthe amount offluorochemicalproportionin solution. REFERENCES Thisstudywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number Cl 111-2,1985. OTHER Last changed: 6/26/00. ENVIRONMENTAL LABORATORY AQUATIC TOXICITY DATA SHEET LR TEST TYPE@@ 2g@@@@ CHET-IICALTESTED-&V-,--T/t-2-/ LOT TEST ORGANIS m SOURCE/./ n TE REC,N zz@ AGE OF TEST ORGANISMS:.<24 hrs. old instars (neona/es) NO. ORGANISMS PER TREATPENT TEST VESSEL: 250ml Pyrex glass beaker TEST SOLUTION VOLUME: 200m] nP i@ 6cm TIME STARTEIL -DATE STARTEN TE COPOPLETEO DILUTION WATER Carbon-filtered well water (Temp.,:ZZ D.O. PM, PHJIC-ZI A L6 @p- - @c 50 (95% Confidence Limits) ->/(@0 TEST Initial Tbtal VES.SE Conc. Amount NO. mg/i Added V Oc)nt-ral 24 Hours Tbtal No. Dead 48 Hours D.O. Thtal No. pH ppm Dead D.O. pr-m lo() t C014MENTS: Test Temp. PC Initials& SECONDARY EFF rT-, -TEST V'LSSEL --Nn @ontrol Syuptcms or Peactims TOT@I, NUMBER EFFJ@T -Hr. ENVIRONMENTAL LABORATORY AQUATIC TOXICITY DATA SHEET TEST TYPE@6-4 CHEMICAL TESTEDZ.="- LOT TEST ORGANISM- 2o,4sOURCE naTE REC'RL Oct t AGE OF TEST ORGANISMS: <24 hrs. old instars (neonates) NO. ORGANISMS PER TREAT!4ENT TEST VESSEL: 250ml Pyrex glass beaker TEST SOLUTION VOLUME: 200m] DYPTRH@:6cm TIME STARTED. DATE STARTEN n TE COIOPLETEQ DILUTION WATER Carbop-filtered well water (Temp.,,L-'@ 3 D.O. ANALYSIIO@- so (95% Confidence Limits) Pm, PH TEST initial Total VESSEL conc. Amount NO. mg/l Added * Contro 24 Hours Total No. pH Dead 48 Hours D.0. Total No. pH ppm Dead D.O. -4 Test Tem. OC Initials& I)ate - - COM ENTS:* SECONDARY EFF .TEST @'C'SSEL Nn @mtroi Symptcms or Reactims TOT@I -HNrUM.BER EFFJ@T ENVIRONMENTAL LABORATORY TABLE 1. SUMMARY OF ACUTE TOXICITY OF TO DAPHNIA MAGNA UNDEP,- EXPOSURE CONDTTT( CHEMICAL CONCENTRATION (mg/1) .CONTROL OC) 24-HR. PERCENT MORTALITY (2) EC (95% CONFIDENCE LIMITS) 50 ing/i > (1) (2) Data were averages of two replications for each concentration with ten replicate. A total of twenty daphnide were used per concentration. EC 50 values were calculated using logistic regression sethods (Problt A