Document X7KxMmwoGgQKgjDYm4XdRrMa4
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CENTRAL SCIENCE LABORATORY
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STUDYNUMBERBTSq0n
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ANPAECRUFPTLOEUTOACRSOOSNOITCUATMCSATANLETSToU(rPLFFeOOSyN)A:sTEt,un
0
_ WITHTHEHONEY BEE
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APNEARCFULTUEORCOOONCTTAACSNTTEUSTUDOLXYFINCOUINMTABYTEESR,T:UPHDOTYTSA6WS0I1STIHUTMHSEALHTON(PEFYOS)B:EE REPORT
US EPA SE`ROIPEPSTS85N0-uEmcboelrog8i5ca0l.3E0f2f0ec(tdsraTfets)t Guidelines
EPPO GUIDELINE 170
OECD GUIDELINE 214
AUTHOR: Paul Wilkins
STUDY INITIATION DATE: 11September2000
STUDY REPORT DATE: 23 March 2001
SUBMITTED TO: Envir3onMmeCnotraplorLaatbioornatory
935 Bush Avenue
St Paul, Minnesota 55106 USA
s-- pam iiee EnCveintrroanlmeSnctiaelncBeioLlaboogryaGtroroyup YorkSYanOduHut1t1o2n, UK
ReportNo 1 of6
Environmental Laboratory Project Number U2723 Page 10f25
00004
- CSL Study Number HTS601
id
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GOOD LABORATORY PRACTICE COMPLIANCE STATEMENT
Is
Study number: HTS601
`>
|
Report ile: Perfluorooctanesulfonate,
the honey bee
Potassium
Salt
(PFOS):
An
acute
contact
toxicity
study
with
1hsJ 1 the undersigned, dechre that he abecives id down i he protocol were achieved and ht the
li 3e i
cdhaetamigceanleraantaeldysairse. vTahlied.rAepsorsttaftueldlyinatnhde generated inthe above study.
apcrcoutroactoellynoresfalmcpcltsesthoef
pdroosciendgusroelsutuisoensd
waenrde
tchoellrecatweddaftoar
d+aa
i
regulactiyons asrse icn oaccmorsdanicnecwiolhtthee OriganisiatioUnKfoGrLEPcoRneompicinCos-op1era1t9io9n 0a5n,d 3De0v1e6l,opTmoenet
-
le
(OECD) Principles of Good Laboratory Practice (1997 ENVAMC/CHEM.
understanding that the UK and OECD regulations meet the following standard:
(98)
17),
Tt
is
my
1
TM
Japan Ministry of Agriculture, Forestry
Production Bureau, (Tokyo, 1984).
and
Fisheries,
59
NohSan,
Notification
No.
3850,
Agricultural
lEed Tchhaeractteesrtiziatteimon wwaass pneortfocrhmaerdacatcecroirzdeidngintoa3cMcorSdtaanncdeardwiOtpheraftulilngGPLrPocecdoumrpelsiaanncdeM:ethhoowdesv,era,ndtahlel
i: m
rreacwhadrataactarriezebdeining maacicnotradiannecde iwnitthhe 3GLMP.arcNhoivessa.mpTlhees Sopfontshoerdhoassinsgatseodlutthiotnstheweerset ciotlelmecitsebdeifnogr.
chemical analysis.
Ji
le
=iy Ie
Signed:
4
fbn
SMtrudPyWDiilrkeicntsor Environmental R&D Team, CSL
Date: 23(03 [OV _(adimmyy)
+e Environmental Laboratory Project Number U2723
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CSL Study Number HT3601 al
`QUALITY ASSURANCE STATEMENTS
`Quality assurance inspections of this study were made on the following dates:
Date Activity
0809000 R1a10n9g1e0t0est
12/09/00
140900
Veriicaion of study pan
Dispensing
Preparation
(est item and
of dilutions
tose
reference)
DAsosseisnsgmbeenetss
Assessments
D1e8f0in9i0ti0ve test Dosing bees
W000 Assessments
Date reported 0809100
1509000
15/09/00
15/09/00
2109000 2108000
sigeCle 8. amoreLs... DucAB BL 0. caimmyyy
Mis CV Walker CQeAntrAauldiStcoirence Laboratory his report has been audited by the Quality Assurance Unit of the Cental Science Laboratory.
sma. Chena Des or. Da23t0e3 .8..carmmsyy
Mis CV Walker CQeAntrAauldiStcoirence Laboratory
Le Environmental Laboratory Project Number U2723
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CSL Study Number HTS601 REPORT APPROVAL
QL
SPONSOR: 3M Corporation
i
TITLE: tPheerfhlounoeryoobeeteanesufonate, Potassium Sat (PFOS): An acute contact toxicty study with
CSL STUDY NUMBER: HTS601
-_-- STUDYDIRECTOR: .
Allis
DATE 25030] (timmy)
MEnrviProWnimleknitnasl R&D Team, CSL -_-- Approval for Report issue
GEMENT.
00000
Ma,
DATE.
22. 2.00 (dd/mmiyy)
MHeraMd EHnvBireownmental Biology Group, CSL
=
Environmental Laboratory Project Number U2723
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Page 4025
990002.44
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L CSL Study Number HT5601
Q
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`TOA FCB ONTL ENE TS
GOOD LABORATORYPRACTICECOMPLIANCESTATEMENT.
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QUALITYASSURANCESTATEMENTS... san -------- wns
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REPORT APPROVAL...
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i TAOFCBONTLENTES cee
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TM
OBJECTIVE...
od
--
EXPERIMENTALDESIGN...
I
MATEARNIDMEATHOLDS.S..
---
|
TOKE FEFETENCE coon
7.
CORUIR OHENSSS.
=
------
-
stn wo rss --
sss--
sesesmmm----_0
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DOSEQUMMSERON ccs -
-n
TI
Observations... esvesesesses sess -
1
AIS O08 rss
----
sensesese-- wren1s
1
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.
==
=
CONCLUSION.......... Table 1. ContactLD4gof
ssm-------- wsmm----
PFO..
ssssstimm----------
"Tabl2e. ContacttoxicityofdimethoBt 1X E1eTENC vrs -
n
Environmental Laboratory Project Number U2723
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CSL Study Number HTS601
REFERENCES........ mm
" " rms
l3
PRINCIPAL WORKERS........
ms ----------------
----
13
DISTRIBOUFRTEPIOROT.N..........
crnsssmm-- smb
APPENDIX | (TABLES AI-A8) DETAILED CONTACT TOXICITY TEST RESULTS.........
lS
APPENDI2X INTERIM CERTIFICATEOF ANALYSIS FORTEST[TEM..........
worm
:
Environmental Laboratory Project Number U2723
Page 6 of 25
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SPONSOR:
[SPONSOR'S REPRESENTATIVE: [ALNODCAATCIOONPYOOFFSTTUHDEY,FIRNAALW DATA IREPORT: SL STUDY NUMBER:
T ITEM:
STUDY:
ST CONCENTRATIONS:
T DATES:
[LENGTH OF EXPOSURE: [LENGTH OF TEST:
T ORGANISM:
SOURCE OF TEST ORGANISMS:
CSL Study Number HTS601 SUMMARY
3M Corporation 9En3v5iBrounsmhenAtvaelnuLeaboratory SUtsPaaul, Minnesota 55106 Ms Rochelle Robideau CSeanntdraHlutStcoine,ncYeorLka,boYrOat4o1ry1LZ UK
HTS601
Perfluorooctanesulfonate, Potassium Salt
(FOS) (PePrFfOlSu)o:rAoonctaacnuetseuclofnotnaacttet,oxPioctitayssstiuudmy Swailtth
the honey bee
9.N3e0ga,t4i.v2e4caonntdro1l.93 ,uPogsitaiivebceonetrol, 45.0, 20.5, RDeafnigneittievset:tes1t2:-1168-S2e2ptSeempbteermb2e0r002000
Single dose 96 hours
HONEY BEE (Apis mellifera L)
National Bee Unit
csL tonvor v081 12
Pr
`ONTACT LD30 (96 hry:
478 ug aifoce (95% CL 3.8 5.8 pig ai bee)|
: .
STATISTICALLY DETERMINED INO OBSERVED EFFECTLEVEL:
CONTACT
193
ngaifee
Le
Environmental Laboratory Project Number U2723
A=y=.
Page 7 of 25
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CSL Study Nulmubmebrer HTS601 6
INTRODUCTION
3lI{e 3MStTehsipfstCoesumotlbupedpyroo0wr2ari2ss4oc1acnrSrahieetpedeComuSbtwLebryfi2ta0ih0hie0s.tEnTivhniderySoopnrwnmoidetlnloHctoaallt,aoRp,&retDYodoeTrelkama0,meCnedTnmoteanetl,Seaiw drao sr, fl mr ove((C0SAo L)fdotr
i months afattrerisssuubemoifstshieonionfathrepofritn.alrepo+rt.The test fenmthwiiel CbSLdidGisLsppoPossesedcohfbeyTCrSLiwirthiisn tntowoof
|
I. y
OBJECTIVE
The objjective of thiis study was to evaluate the acute contact toxicity of Perfluorooctanesulfonat,
i
Potassium Salt (PFOS) administered to the honey bee (Apis melifora).
erosion,
i3
- |
|
177 Is 1li o
1s
JT e -
zm
de
7 =
de
EXPERIMENTAL DESIGN
C0ontthaectboedxypossuurrfeaceev.aTluharteeedbcauttcihcuelsarof`abbeseorsptiinongroofutphse totefesstt10titeebmemesfowlerleowfoillnogwtiongpitchaellyapdploisceatdioonnofhea drophleest
with 1ul drops containing PFOS in acetone.
"
RGR
pate
Tsseeopoamdreeattteseridmciabnlyeyaasfnpcaatcpoeprdroodxfoitsmeeanstweoeftroexthiacditmtyien`saatirsaaitngtneeer-gfmeidnedwitenogrfgeinrdotiunhgpeetnesssttowawfdaam3ssi0nipyseotruefnroergdmaedd1u.0ltgFrowouourrpkdseorsoefhso3no0efytyhobeueencsg.taFiditveiem
woOfrkt1hee0rt9`e6hstontheoeyumrbsaeepfspotlroitddoextfiictnoheleotgthihecoaLrlDasxroe.wsiptAohtn3sienmisiticaartnoid-onapaopnfltiLhceaDtteostpv,eeteaec.h3bneTdehe9re5bce%eeisvcewdeorassnihnognlneotlboepmeicrsaelcwddofesoe
calculated. All doses of test item are reported as ug active ingredient (a. bee. At the end of cach
tbeystinacniynbeeraetsionr.emAalilnidnagtaaalriverwetearienekdilolnedthbeysfwrdeeyzifneg.and all bees used in the study were disposed of
Thedos sitet rechown Slow: Morality 1nd sublet ft erased 4. 24,45,
Gagne Range finding contesttd|oaselcevetls
gaisbee)
(ug aifbee)
T 2Ts e -- T01 s o5x 7]|
eTww
Trion X100
-Lo
=
Environmental Laboratory Project Number U2723 Page 8 of 25
000048
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CSL Studyly NuNumber HTS601
-Te Denicomut todo
TS pee Gnnas |
Ly sa ia ] a e
Te
nCiITn]"
`3
` Et ofL; De valueswere cael rte defini GnEat es.Tb Toe racksord
= ing
eeweirengdeeti--ceorromimnnein--dlblysyah--stecatniostciDncaolgnev41ealutat)ioioynn ow)efotrhoerdmoritavlinteey daoota.nnelSgoontgs((Giimlnhstoe us BBAASESE400
=--
TGGehdeuurmieestnhsopdescO,iPfisPeTedSiinsNaUSb.eraEnnBv0i3rroM ntmenA otfaHsloT nPiersE otsBciAtR osiiNoohnDI enAnMgsA dEenTa cL HyedsrOSDS esrSiienonset8hiy5ss0 e<B(reCopools oorgteiobEnasfsBecidshpsaonnTBopseor
ZDT aae orCmoernetoCiuetainsdbsaoernweTGsexnLpOpeDetlTnodkyaofre ene1itooemnasesdCoSActioeetCnoCTooalsitTlgooursi(,taetN:aoETPearPrhO.GIruPniprodeorendlduitentaereon
ce E SonoR 15 or Lo 2 3 5 Co epee Lon i is oc
Ton. snmormsan tr pis aphorne et em i is kley
ba)
absorption or from grooming.
Pe
Fos citi
isin fue
da
Testitem
iy
.Le
l|s
T{rahkeaCPtAFSeOoS]Nu(mLIibUeoPrAr2C1Nb9a5m3le9TT:o1oEu-fwOhc4fhtiatonremeCspuElofwoodneeircp,achlioidit, 2e1a1,7.1,)2:s2l.Te33od,4,ie4n,5.t5i,6so,6.s1,ua7d,,8y.8:w8ashspeplstuaipdepelcr daifehdbuoybryoWst Wiilddliefe
==e
PFOS was dispersed in acetone (analytical grade) before use due to a low solubility in water
T:= ET IGTpHpraonnimeaEtseOlSyToSv0e0ahmgS/lo)a. Amddispoefrssmieonvtest was caarrPieRod oOuteN, ,befc coroenctehnemttaoixiocnitay teistsswe1rSedeepelrmfvoeermseldh,e
=
Environmental Laboratory Project Number U2723
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CSL Study Number HT5601
gi
used t0 include material which may
PFOS were checked visually during
be suspended ordispers
dispersion tests before
ed.
the
Homogeneity
day of the test
of such
and imm
solutions
ediately
ofthe
before
pusuer.posAleol fsoaldumtiinoinsstrwaetrioen.re-Tmhiexetdestpriitoermtofousrem.edSaolculteiaornssoolfuttihoen toenstmdioxsiensgwaetrtehehohmigohgeesntoduossef,orafttheey
w(tohe haopuprrsoxtihemraetewacsoancesnltirghatt abeceestoinnecnoengtaalcteddotshienngeteedsts1.0
ipsoeenrdfiromereqmnutiarnaetdarptpooloipmcraettpeiamorpneetretashtteuarhseigaahcteesattocnodeniclseuontletudrtaidtooinsosnecoaifnn
8t6h.e0teustg).a.i Tuhle uPsFeOoSf be readily applied 10
Toxicreference
Dimethoate toxic standard NBUSO, BASF 40 lot 37M 9020106 (a blue liguid EC formulation, 37.4%
w/w ai, 400 g/l
stored at 0-11C
nominal
(on one
occocnacseinotnratthieonm),axwiamsupmurtcemhpaesreadtufrerormecUorAdePd
York on
was 14C
door to the refrigerator had been opened) suitable to maintain stability for 2
14/06/00
- this was
and was
after the
years according
cionnfcoernmtartaitoinonsubpapsleidedonbydatthaesmuapnpulfiaccdtubryerB.ASFD.ose and LDso calculations are for corrected dimetheatteo
Testbees
sTouhbesntohannottanireagyletbdeaietnas(eAbcptasissesmioennlcleitfhiteeriase)fafinesctuismoepffouraltgairnnotcephvoealmlliuiacntaailtnsogrutophfeovnpaorbtieeoneutssiawaligrhtihaczuawlrhtduisrcaholfctarogorcpiasct.uelgtTouhrreiazrleechpioetmeainlctsaiolaals hazards
`Worker honey bees (Apis mellifera) were obtained from colonies belonging to the CSL National Bee pwUrnhiiiotcr.hBtobeeeteshsefwrsetoarmret tohtefakcteonhlohtnaeysdt 3na2ontwdbeserheenouwstnreedtaotienbdtewheiftrrehaenagoefv-aafrciranordaiicnniegd,eannwodistdeheimfnaintiahtneidvleaastmtesot4e. bwaBe.eeekTssh.weeWrcoeorleokxneayrmifbnreeoedms wawenerdreetrpcaolnlaslcfeeecdrtreiidnntgforttohhmeemtihn(ec4u0hb-iav5te0orbpye(r2u5csia1ngge82)aCi,sn6tmoa5lc.lyla5imn%dorurinecltaaltoimfveessmhhoukmceiad,giesgt.eyn)tuIlnnytitslhheankeliaenbdgoerdtathfoeorrmytfhterhoetmemsttohsehccoamgbess
`Conditionsduringthetests
mTcoeensattsauibrneiiennsggwtaehperpesruohcxroioumsaseteedsloyilnut9tieocsntmwcaihsnamadbfifeairmxseetdewrih.niecahAchwescrmheaalmlbceleria.nnv,eErwtaeecldhl ptveeesttnrticihldaaitmsehbd,e(riancpvopenrrtotaxeiidnmeapdteer1li0y dw3iosrhkmeelsr),
kbeepets iannddawrkenreesisdeatnt2i5fied 2byCsatunddy6n5u%mb+er,5%dohsuamgiedigtryo,upexacnedptredpulriciantge.obsAefrtveartidoonssi.ng,Tetmepstercaatguesrewaenrde
relative humidity within the environmental chamber were recorded continuously during the test.
Doses of the 904 ug a.i/p
test item we l (definitive
rteestm) aPdFeObSyapnrdepmaarkiinnggaassoelruiteisonofofdil1u0t3ioungs
a.i/al (range-finding from these. All dose
test) and solutions,
Were made in acetone. One set of control bees was treated with acetone and a second set of control
Le =
Environmental Laboratory Project Number U2723 Page 10 of 25
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CSL Study Number HTS601 al
5
tS1et&so)/ckdTiesmioetltouhnsoiaoXnt-se10oi0nf.dtehSietoonpcioskseidstoiwlvuaettiecornoscononf,ta2.i8 ndiinmugegt1ha/oit1fe,T(rriwatenorgnee-Xfp-ir1ne0dp0ianrgweedtreesitn)prdaeenpiadoren3di..s0edpgwaatielr
containing
definitive
` i
ftorreaPtFeOdSw.ith 1 g/l Triton X-100 in deionised water. The tests were carried out in pCaornatllreollwbietehs twheorsee
i Doseadninistaton
i> IL
toThnheteothbfoielretasexrwpweairpteehraaanna1 deIsctohduertnoit.pseedodfiwnatiogtihtvhecenaprtebetotin iddtiiesomhxiccdoaengceiemn(tdmrreaodtniieaostnewleyrebedfiosrcearddeods)i.ngEaancdh gbeenetlwystihppeeddoaunt
l7e
iPlnucsubmaitcorropwiiptehtatceo.ntTihneuoluidswsauspptlhyeonfp5l0ac%edw/ovnatqhueecoaugse,sutchreoobseeess1olIaultliaoocwneetadosntfeoouordse.icnogvearn aAnndackheeprtsinEtBhPe
3B rmr oni Mortality and any bees knocked down; i.e. alive but immobile, or stumbling were assessed at intervals
Analyofsdiatsa
I|
Analysis of the data comprised plotting probit mortality recorded after 24, 48, 72 and 96 hours against
1= i
cttoohentfhlieodsgee.anrcietTholmixmioicftisdt,yoNsweOa(EsCLSeLxapnPrdreossbslieotdpe1aspoafLctkDhasegoer)ie.nsppAognsloeeafsctaucr-tvsievq.euTarihenegsrNerdeOigerEnetsLsipoe(nrno(bFeoiebnsn(ee2ryv4e)1d9w7ei1ft)fhewcttahsleefv9iet5ltse%)d
lw
were estimated using Student's test (p<0.05)
um
cTahteegLorDiieos
value used
was were
used
to
classify
the
toxicity
of
the
test
item
according
to
the
ICBB
(1985).
The
>
Highly toxic:
less than 1g airbee
=
i
SMloidgehrtalyteolxyict:oxic: Virtually non-toxic:
11-100 p-gpa1giafb0ieef.0. greater than 100 ug aibee
Te
probit program (Prob 1, version 4)
TB eet se ettTa tpt Ae : oe
RESULTS AND DISCUSSION
L3 e
tFhuerthhoenredyebtaeielsoafre4g.7i8viegn ab.eilboewe.. The contact toxicity of the test item resulted in a 96 hr LDsvalue for
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Rangetest
CSL Study Number HT5601 1]
:
asMunobrdlteaatlbhioatlvyeesfifwgeinctithfsiawcaesnrtteeleyopgbrdseoeasrteveerrdetashtapnaonnssyeeednobseietnwlteeheveenlcaondtoroslesowfas10o.b3saenrdved10a3t palgl ad.o.s/ebese.ofN1o0.3deisge ar.el/abteeed
=1 ) Definitivetest
iI`w
`4T.h2e4reanwdas9.s3ig0niufgicaa.nitbmeoer.taTlihtey a9t6alhlrdLosDes awbaosveca1l.c9u3lajtgeda.ais/b4e.e78wiptihg aa.sit/ebeepPep,dowsitehre9s5ppo%ornsceonbfeitdweenecne
ii w
lNiomidtososefe3l.a8t-e5d.s8ubplgetah.alibeeffee.ctTshweerNeOoEbLserwvaesd 1at.9a3nypugdoas.eibleeveelat 96 hours (Students t-test p<0.05),
iiwd T"Thaeble242,. 4M5o,rt7a2liatyndof96be-ehsouerxpcoonsteadcttoLtiheotovxailcuerseffeorrendciemientthhoenetesitns tahlelodweefidncitailvceultaetsitonaoref sahcoowntnacitn
|
LDsoof0.19 pig ai dimethoaterbee at 24, 48 and 96-hours and 0.20 pig ai dimethoate/bee at 72 hours.
Ee J
Tbeheessewerreesurletsacstihnogwnaortmoaxlilcyitiyntlehviesltewsitt.hin the ranges reported by the OECD guidelines showing the.
T
i
CONCLUSIONS
le 1 Nid
NEsixOgpnEoisfLiucrbaeanstoemfdorohtnoanlseitayttybiesatebiscoavlteoanP1aF9l3OysSisgobfya.tchioebnedtaeatcaatn(ddSotasuid9en6ngt-'hrsoeusturtletLsetDdipo<i0nc.aa0l5cs)utlweaeatpsedd1oa.ss9e34u.r7ge8s.ppiognbseaeo./acbuter9ev6.e hwTrisht.eh
7
the tetreistuelmtsisdecmloanssstirfaiteaesdthmeodteorxiactietlyyotfoxPiFcObSy caosn4t.a7c8t pg a.i/bee and according to the ICBB (1985)
7
ble1. Contact LD.of PFO
ES
Time
LD,
95% Confidence | Estimate of slope of
=
Hrs
2
pg a.i/bee)
17 50
Interval for LDyo
282-712
esponse line
| Te
[eT i0a Twn
| 82-130 | 47-76
[20
, Ie CeTam] 38-58 [| a0
Ly
iy
Time
the
Hrs
(ig aisbe)
2Top
'
aTop 7
a
I
" i CoeToro]
55% Contidenes
Interval for LDsy
016-024 016-024 016-024 015-023
| EtmeoT ge oF
response line
[ar [as
=
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ost sudy Number scot feyL
REFERENCES
European and Mediterranean Plant Protection Organization, 1992. Guideline on Test Methods
for Evaluating the Side-Effects of Plant Protection Products on Honey Bees. EPPO Bulletin, 2,203215.
Finney, D. J. 1971. Statistical Methods in Biological Assay, 2nd ed., Griffin Press, London. International Commission for Bee Botany (ICBB). 1985. Recommendationsfor harmonization of
methodsfor testing hazard ofpesticides to honeybees. Third Symposium on harmonization of methods for testing hazard of pesticides to bees, England. Organization for Economic Cooperation and Development. 1997. Guideline 214 Honeybees,
US. Environmental Protection Agency. 1996. Honeybee Acute Contact Toxicity. Series 850Ecological Effects Test Guidelines (dra), OPPTS Number 850.3020.
STUDY MANAGEMENT PRINCIPAL WORKERS
Study Director
CSL Management Representative
Mr P Wilkins
Mr MH Bew
OPERATIONAL SUPERVISORS
Principal Scientist Scientist & Test Item Controller
Dr HMThompson Miss UB Pietsch
Scientist & Deputy Test Item Controller Scientist
Miss S C Green Mr A H Battersby
Scienist Scientist
DrR Spinks Mr MJ Kelly
QUALITY ASSURANCE
ae QA Manager
oMrstR MeBrokokes
Environmental Laboratory Project Number U2723
Page 13 of 25
000053
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7
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CSL Study Number HTS601 Si
DISTRIBUTION OF REPORT
`SSppoonnssoorr''ss MRoepnrietsoerntative Study Director
MMsrRDRoPbalimdeerau 2 ccooppiyes
MrP Wilkins
1copy
QCSALMMaannaaggeerment Representative
MMrRMMH BBerwookes
I copy 1 copy
I
Environmental Laboratory Project Number U2723
:
Page 14 025
060054
9 CSL Study Number HTS601
|
DETAILEDAPCPOENNTDAICXT1T(OTXAIBCLIETSYAlT-EAS8T) RESULTS
I
Table AL Results of range-finding contact dosing test with PFOS - Mortality
1! 3.
Actualdose Cage
(pgai/bee) No.
Cumulative number dead (n=10)
iI
p 4hs 2r p4rh ---- @he s----7--2bp n----9r 6h
I
103
13
0
6 10 10
10
i
i
101033
114s
00
75
1100
1100
1100
P moose 01 i?
}
103 103
16
0
7
0
0 0
4 3
5 5
7 9
TM
-1?
2
110033 EF19 0| 01 0Y 0: 01
103 21
0
0
0
0
0
|
0.103 2
0
0
0
0
0
Iig
00.110033 22%3
00
00
00
00
00
i
1a5
oo
o
226
2
0
01
0
01
0
01
0
01
21
0
da i 4
Gm 0 oo oo
> -
[2]
29
0
0
0
0
0
o
30
0
0
0
0
0
1
*undosed
+o =
=
--_--
Environmental Laboratory Project Number U2723
Page 15025
000055
To tion ce 9 CSL Study Number HTS601
ii Table AZ. Results of range-finding contact dosing test with PFOS - Sub-lethal effects
i! i?
(sgai/bee) No. -- Tahms Num-- be2r hknock-- dohwns(K) or-- stTumhboleminge-- e(5)Spg6ehcmes
`i
110033 114
00
x0 00
00
00
iI 103 1s 0 2 0 0 0
I
110033 1I6
00
00
00
10K
00
i
103
18
0
0
0
0
0
> |
110033 129
00
00
00
00
00
iw
103
2
0
0
0
0
0
ii
00.110033 22
00
00
00
00
00
I
0.103
2
0
0
0
0
0
pI= J
oo
225
00
00
00
00
00
>
o
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0
0
0
0
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i
I2.
20
228
00
00
00
00
00
do
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*undosed
Je
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Environmental Laboratory Project Number U2723
Page 16.0125
060056
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TableA3.
CSL Study Number HT5601
Results of range-finding contact dosing test with Dimethoate - Mortality
gi
we (ugaise) No.
030 |
030
2
030
3
Cumulative number dead (n=10)
2
0
10 10 10
10
10
10
10
10
0
9
9
10
10
00.1155
015
54
6
00
0
5s
3
66
66
66
4
5
6
0o0m75 7500
0075
0
wo
o o
i 2
0 0
0oo0
0
0
0be0
0
0
0 oo
0 0
0 0
0 0
0 0
"dosed with Trion X.100
J+e -- Environmental Laboratory Project Number U2723
000057
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CSL Study Number HTS601
Table Ad. Results ofrange-conftanctddosiinntegst with Dime-tSuhb lothaaleef
[
i
Acwslduse Cage
(gaibe) No.
Number knockdown (K) or stumbling (5)
Whe bs whe The sens
iin
003300
21
2ax%
00
00
00
00
I
030
3
3K
0
0
0
0
=I ] i
0011s5 015
45 6
10K 0
00 0
00 0
00
00
IK
0
i
!
0075
7
0
0
0
0
0
[id
00.007755
98
o0
00
00
00
00
i
o
10
0
0
0
0
0
-
>
0
o
n
2
0
[4
0
0
o
0
0
0
o
0
>
[od=1.
Pn "dosed with Tron X.100
2 i <
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-- Environmental Laboratory Project Number U2723 _
s 000058
9
CSL Study Number HT601
i3
Table AS. Results of definitive contact dosing test with PFOS - Mortality
i
A(cgtauisflbdeoes)e CNaog.e
Cumulative number dead (n=10)
i
hes Zits @hs 72h 96hm
`i
445500 22114 00 73 59 1100 1100
Ii 450 21s 0 s 10 10 10
=I
22005s b21y6 00 43 108 105 1100
i?
205
218
0
H
10
10
10
ii| ?
993300 22109 00
11
35
59
95
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ii>m
a4Bis2 m mmo o0 00oe22 443 5.s
[ied
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119933
22265
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01
01
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193
21
0
1
1
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3
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00
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=1
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BH 0
0
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0
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Environmental Laboratory Project Number U2723
Page 19.0125
i
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000059 oo
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Table AG.
CSL Study Number HTS601
Results of definitive contact dosing test with PFOS - Subslethal effects
rP oo as Nabe aocdown(oss
4brs 24hrs 48h
72hes 96hes
iiy?
I
445500
213 214
0 0
1K 0
1K 0
0 0
0 0
450
215
0
0
0
0
0
PP @ om 0 ooo, Iom
i
220055
22176
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00
00
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205
218
0
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0
0
0
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IP
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993300 22109 00
930 21
0
00
0
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10K
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0
+m
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424
23
0
424
224
0
0
0
0
0
0
0
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0
| nd
119933
22256
00
00
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00
00
193
27
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bi o@
3231
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00
00
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o
233
0
0
0
0
0
E *undosed
Fx
Environmental Laboratory Project Number U2723
Page 20 of25
000050
9
CSL Study Number HT5601
S TE ---- 3 Table AT. Resultsofdefciontnactidostingitesvtwieth DimethMooratatliety
Id
Actualdose Cage
.
(ug aifbee) No.
Cumulative number dead (n=10)
:
030 201 2
8
8
8
8
ii3
003300
220023
31
65
160 160
170
iI
os 204 1
3
3
3
3
ii s
0011ss 22056
01
45
4s
45
45
i
00007755
220087
00
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01
01
01
0075 209 0
0
o
0
0
i
-
0
210
0
o
211
0
1
0
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0
2
0
2
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212
0
0
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1
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Environmental Laboratory Project Number U2723
Page 21 of25
000061
9
(CSL Study Number HT5601
`> i
Table AS. Results of definitive contact dosing test with Dimethoao te --nSub-lethal effects
i" n
(Acutguaaildbdeoes)e CNaog.e
Number knockdown (K) or stumbling (5)
I>
4 hrs
24 hrs
48 hrs
72 hrs
96 hrs.
|i Ie
003300 0201 00 I0K 030 203 IK o
00 0
00 0
00 0
[
i
015
204
0
0
0
0
0
ii n
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220056
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0
0
im 1
oo o
221101 22
10K
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000
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Environmental Laboratory Project Number U2723 Page 22 0f 25
000062
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Senacai SigCris: Fon 106
Reassessment Date: 097101
-- o033r% d, 035%NMRimpaiies,| 50 orgie ad mpi, JE%POAA, PToutrailtiympu=r1ty0f0r%om- 1l3.0e9t%s==8163..50%9%
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do"itSuelmnfuirnionataichioerosnnatmgmtephtlahedoaedpncnpoeenadrniistitoocnbnsae.lcTosnh,veeesrnntdieiondngtroecsoSunOlitadanigdraeehse(waseldiewtieetcrhttpehrdosumssuiilonlnogrt.heBased
onthe reals,th SOs otconsideredsnimpury.
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Pentaluoropropanoic acid
"Theoretical value calculations based on the empirical formule, CiFysSOSK"(MW=538)
"TisworkwasconductedunderEPAGoodLaboratoryPracticeStandards(40CFR 160),
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Environmental Laboratory Project Number U2723
Page 24of25
_ oie
PLa E 20,
Tn QF 000064
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Environmental Laboratory Project Number U2723 Page 25 of25
000065
CSL Study Number HTS60] CENTRAL SCIENCE LABORATORY
AMENDMENT TO PROTOCOL
Study title
PcoenrtfalcutorooxoicitatnyesStuuldfyonwiicthAtchide,hPoonteayssbieuem Salt (PFOS): An acute
`Test facility
Environmental R&D Team,Environmental Biology Group Central Science Laboratory, Sand Hutton, York, YO41 1LZ
`Study number HTS601
Amendment number: 1
Number of pages: 2
.
Study Director
Paul Wilkins
.
SignaturLe dlly
ye
ManagementRepresentative Medwin Bew
Signature |/] hfean
Date_1u-2.07 (dd/mm/yy)
Environmental Laboratory Project Number U2723 page 1 of2
000066
CENTPRRAOLTOSCCIOELNACMEELNADBMOERNATTORY Amendment No 1 to Protocol HTS601
AcfhtaenrgecldafrriofmicPaetrifbolyunotrhoeocStpaonnesoSru,lfiotnwiacsAcriedq,uePsotteadstshiautmthSealTte(stPFIOiSem) ntoame be cPherafnlgueodroforcotma:nesulfonate, Potassium Salt (PFOS) and consequently the study title
i3
i
:tPheerfhlounoeryoobceteane Sulfonic Acid, Potassium Salt (PFOS): An acute contact toxicity study with
= E]
Pheornfelyuobreeo.octanesulfonat, Potassium Salt (PROS): An acute contact tricity study with the
`This has no impact on the Study
Circulation list
SSppoonnssoorr''ss MRoepnrietsoerntative Study Director CCSSLL.MQaAnaUgneimtent Representative.
MsR MrD
Robideau, 3M Palmer, Wildlife Intemational
Mr Mr
P Wilkins, CSL M Bew, CSL
Environmental
R&D
Mrs R Brookes, CSL
Environmental Laboratory Project Number U2723 page 20f2
000067