Document Vjq7n6dx27JZ4OEpkL9bMMzX4

ACTIVATED SLUDGE RESPIRATION TEST SUBSTANCE INHIBITION TEST Identity:Perfluorooctylsulfonadtied,ecyldimethylammonium salt;may alsobe referredtoas Fluoroalkyalmmonium derivative[.1Decaminium, N-decyl-N,N-dimethyl-s,altwith 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafl-uoro-1 octanesulfoniaccid(1:1),CAS # 251099-16-8] Remarks: The 3M productionlotnumber was Lot 1. The testsample is L-14394 referredto by the testlaboratoryas P3025. The sample was labeledF-11615, Lot 1. The testsample isa mixtureofthe testsubstance .inwater (approximately30-40% testsubstance,60-70% water,and 0-5% ofresidualperfluorochemicals)A.llvaluesreportedrelatetothismixture. The testsample appears to be a 2-phase dispersion(clearliquiwdith opaque solid)which rapidlyseparatesafteragitationN.o calculations were made toadjustfortheactualconcentrationofthetestsubstance in the testsample. METHOD Method: OECD 209 Test type: Staticacute GLP: No Year completed: 1997 Analyticalmonitoring: Nominalconcentrationsp.H, temperatureand dissolvedoxygen. Exposure period: 30-minutesand 3-hours Statisticamlethods: notapplicable Test organism source: Activatedsludgemixed liquorcollectedfrom Metro Wastewater Treatment Plant,St Paul,MN Inoculum mixed liquorsuspended solids: 4.38 g/L lnoculum holding: None - used the day itwas obtained Test conditions Dilutionwater: MilliporMeilli-Q@w)ater Synthetic Sewage: Per OECD method Reference and testsolutionpreparation:A stocksolutionofthe referencesubstance,3,5-dichlorophenowla,s prepared by dissolving500 mg in10 mL of1N NAOH, dilutedto30 mL withMilliQ@) water,then broughttothe pointofincipienptrecipitatiownith1N H2SO4, and dilutetdo1 LwithMilli-Qw@ater.The pH ofthe referencesolutionwas measured tobe 7.3. The testsolutionswere createdby directadditionofthe test substance tothe testvessels.Aftertestsubstanceadditiont,he vesselswere stirredwitha Pyrex pipetteforapproximatelyone minute. A whiteprecipitatweas seen on the bottom ofthe vessels afterstirringT.he particlewsere suspended duringaeration. Vigorousshakingwas done beforeremoving each sample aliquot fortesting. Test vessels: 1200 mL Pyrex@ FleakerSTM containing500 mL test solution Number of concentrations: 3 plus one referencecontroland two blankcontrols Temperature: 19-21OC Total Suspended Solids and pH for sludge on day of testing: 4380 mg/L and 7.87 respectively. Element basis: respiratioinnhibitioans determined by oxygen consumption Method Remarks: Test temperatureswere notcontrolled.The respiratiornateswere normalizedto200C forthe inhibitiocnalculations. RESULTS .Nominal concentrations: Blank controlr,eferencesubstance control(10 mg/L),100, 500, and duplicated1,000mg/L testmaterialsolutions Element Values: 30-minuteECso = >1,000 mg/L 3-hour EC50 = >1,000 mg/L Element value based on nominal concentrations AnalyticalMethodology: Analysisof DO concentrationsinalltest solutionswere performed usinga dissolvedoxygen meter. Control response: Satisfactory Remarks: No oxygen inhibitiosneen inthe 1,000 mg/L exposure concentrationrelativteothe controlinthe 3-hourexposure. CONCLUSIONS The testsubstanceexhibitedonlya slightinhibitoreyffect(<20%) upon respiratioanta nominal testsubstance concentrationof 1,000 mg/L atthe 30-minute exposure and no inhibitoreyffectatthe 3-hourexposure. The EC50 (respiratioinnhibitioins)thereforegreaterthanthe highestexposure concentration1,,000 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking2. Testingmeets allcrite@aforquality testingb,ut lacksanalyticaclonfirmationoftestsubstance concentrations. There isa lackofcharacterizatioonfthetestsample. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory,St Paul,MN, Lab Request P3025 OTHER Last changed: 5/25/00 3M ENVIRONMENTAL LABORATORY INHIBITORY EFFECTS OF P3025 ON ACTIVATED SLUDGE. RESPIRATION OECD ActivatedSludge RespirationInhibitiToenst,# 209 STUDY COMPLETED: January21,1997 FINAL REPORT COMPLETED: May l2,1997 Env. Lab Request Number P3025 Prepared by: Name: Title: Laboratory: Address: Susan A. Beach Advanced Environmental Biologist 3M Environmental Laboratory Building2-3E-09 935 Bush Avenue St Paul,MN 55144 ActivatedSludge RespirationInhibition Page 1 May 12, 1997 SUMMARY The purpose ofthisstudy was toevaluatethe inhibitoreyffectofP3025 on aerobicsludge systems inaccordance withthe *ActivatedSludge RespirationInhibitioTnest",OECD No. 209. The median effectivceoncentration3,hour EC50 was reportedas > 1000 mg/L. INTRODUCTION Toxicitytestswithaerobicsludgeprovidea rapidscreeningmethod and are reliablperedictorsofthe inhibitory effectsofpotentialcontaminantson the operationofactivatedsludge treatmentsystems. Thistoxicitsytudy measured therespiratiornateofactivatedsludgeobtainedfrom a municipalwastewater treatmentsystem and followeddecreases inthe rateofoxygen consumption over a relativelsyhortperiod(30minutes to3-hours)inthe presence of differenctoncentrationsof P3025. Test Substan= Appearance Aqueous Solubility AvailablePhysicochemical Properties: Date Received Storage Test Solutions Reported values MATERIALS AND METHODS P3025, date 10/7/96,Lot 1 Two-phase dispersion(clearliquidwithopaque solid) Apparentlynot completelysolubleinwater attestedconcentrations. BoilingPoint: Vapor Pressure: Vapor Density: EvaporationRate: Solubilftiynwater: Specdic Gravfty: Percent Volatile: pH: 10/16/96 1 OOOC ca. 18 mm Hg @ 200C <1 <1 Insoluble ca. 1.2 60-70% 6-8 Inthe dark atambient room temperature(221 20C) Test substance weighed out and added directltyotestvessels.Aftertest substance additionv,esselswere stirrewditha Pyrex pipettefor approximately one minute. Test substance formed whiteparticlewshich sank tothe bottom of the FleakerTM. Particlewsere suspended duringaeration. The reportedvalues are based on nominal concentrationsinthe testmedia atthe beginningofthe microbialassay. Controls: FunctionalControl (Referencesubstance) 3,5-dichloropheno(l3,5-DCP). Aldrichred label;97%. Lot 09615KG DO. Received 11/18/96. ActivatedSludge RespirationInhibition Page 2 May 12, 1997 Controls(Cont.): Blank control (untreated) Two controlscontaininginoculum and nutrientmedium, but no testor referencechemicals,one atthe startand the otheratthe end ofthe testseries. tnoculum/NutrientMixture: MicrobialInoculum NutrientMedium Activatedsludge mixed liquorwas obtainedfrom the MetropolitanWastewater Treatment Plant,St Paul,Minnesota on testday. The suspended solidslevelwas 4.38 .072 g/L; initipaHl 7.6. The sludgewas continuouslyaeratedafteritwas receivedinthe laboratory. Syntheticsewage feed prepared on testday as outlinedinOECD 209. Exposure: Test Date 1/13/97 Contact Time 30-minutes and 3-hours. Vessels 1200 mL Pyrex@ FleakerSTM Test SolutionVol. 500 mL StartingTest Conc. Test Substance 100 500 1000 mg/L ( induplicateat30-min;3-hourduplicatelostdue tolabaccident) Reference Substance 10 mg/L Mixed Liquor Suspended SolidsLevel inTest Media 1.75 g/L DilutioWnater MilliporMe illi-0w0ater Temperature Lighting Mixing and Aeration 15.6 - 1B.OOC over the course of the study Ambient laboratorylight Test solutionswere continuallayeratedwithclean,oil-freaeir. Measurements Dissolvedoxygen concentrationswere measured after30-minutes and 3-hourscontact time. A sub-sample of testsolutionwas quicklypoured from the exposure vesselintoa BOD bottleand dissolvedoxygen concentrationswere recorded using a dissolved oxygen meter every 30-seconds over a five-minuteperiod.The sub-sample was stirred whilerecordingoxygen. The sub-sample was returnedtothe main testsolutionafterthe 30-minute readings. ActivatedSludge RespirationInhibition Page 3 May 12, 1997 Procedure The testwas conducted ingeneralaccordance withthe "ActivatedSludge Respiration InhibitioTnest",OECD No. 209 (1).Values were reportedtodifferenltevelsof significancdeepending on the accuracy ofthe measuring devicesinvolvedinany one process. CALCULATIONS The reportedvalues are based on nominal concentrationsinthe testmedia atthebeginningofthetest.The concentrationstested and the correspondingresponse data derivedfrom thetoxicittyestare used tocalculatei,f possible,the medial effectiveconcentration,EC50 and the 95% confidenceintervably standardlogistic regressionmethods (i.e.P,robftAnalysis,Trimmed Spearman-Karber). The EC50 isdefinedas theconcentration estimatedto inhibitthe rateof respiratiobny 50% as compared tothecontrolsa,fter30-minutesand 3-hoursof exposure to the testsubstance. RESULTS The percent reductioninrespiratiornateand the median effectivceoncentrationE,C50, obtainedafter30minutes and 3-hours exposure tothe testsubstance P3025 are summarized inTable 1. The percentreductionin respiratiornateof the referencesubstance 3,5-DichlorophenolisalsoreportedinTable 1. The median effectiveconcentration,3-hour EC50 of P3025 to activatedsludgewas determined as >1000 mg/L. The 95% Confidence Intervaclould not be calculated. ACCEPTABILITY CRITERIA This toxicittyestmeets qualitycriteriparovidedintestguidelines: 1) The two blankcontrolrespiratiornateswere within15 percentofeach otherafter3-hours exposure. The percentdifferencewsere 22.2 and 4.1 after30-minutesand 3-hoursof exposure, respectively. 2) The 3-hour percentinhibitioinnduced by the referencesubstance,"3,5-DCP", was withinthenormal range seen withthismixed liquorinthislaborator(yseeTable 2). 3) The reportedvalues were based on nominal concentrationsinthetestmedia atthe beginningof the microbialassay. This testwas conducted by Trudy K. Stiefenhoeferas requested by Pam Wolf of3M SCD Division. REFERENCES 1. OECD (1984). ActivatedSludge RespirationInhibitioTnest,Test No. 209. In:GuidelinesforTestingof Chemicals. OrganizationforEconomic Cooperationand Development, Paris,France. 2. OECD. 1981. PrincipleosfGood LaboratoryPracticeA,nnex 2,C(81)30 (Final)7:-28.OECD Guidelines forTestingof Chemicals. OrganizationforEconomic Cooperationand Development, Paris,France. ActivatedSludge RespirationInhibition Page 4 May 12, 1997 TABLE I SUMMARY OF TOXICITY OF P3025 TO ACTIVATED SLUDGE TEST CONC. mg/L 100 500 1000 1000 (dup) EC50 95% Confidence Interval Statisticramlthod used PERCENT INHIBITION IN RESPIRATION RATE 30-min, Exggsure 3-hours Exl2osure 1.8 0 0 0 17 0 19 na -kb a=k6nt > 1000 Notcahaabie Not applicatse > 1000 Notcak:UabL, NotAmkab4e SUMMARY OF TOXICITY OF 3,5-DICHLOROPHENOL REFERENCE SUBSTANCE To ACTIVATED SLUDGE TEST CONC. mg/L 10 PERCENT INHIBITION IN RESPIRATION RATE 30-m*n. Expgsure 3-hours Exl2osure 36 54 ActivatedSludge RespirationInhibition Page 5 5/12/97 TABLE 2 OECD 209 Results from Reference Toxicant Testing with 3,5-Dichlorophenol Test Concentration = 10 mg/L Percent InhibitionIn Oxyaen Uptake Rate Date Tested 11/27/95 1/25/96 2/26/96 3/4/96 3/7/96 3/11/96 4/4/96 4/8/96 4/17/96 4/25/96 5/16/96 8/14/96 8/19/96 8/26/96 8/28/96 9/5/96 9/9/96 9/16/96 9/30/96 10/2/96 10/9/96 10/14/96 10/23/96 10/29/96 10/31/96 11/4/96 11/6/96 11/18/96 11/25/96 12/2/96 1/6/97 1/8/97 1/13/97 1/21/97 MEAN Standard Deviatiorl 30-min Exposure 38 36 32 27 34 34 29 25 36 37 55 50 54 46 52 48 69 40 36 '44 48 30 28 45 42 55 53 30 52 28 40 49 36 37 41 10.3- 3-hrs Exposure 58 53 58 65 57 58 58 60 56 66 52 73 68 52 57 49 37 -58 - 45 54 61 46 55 59 48 54 64 42 59 50 47 41 54 56 55 7.8 MLSS*, g/L 5.5 7.2 5.2 2.4 3.4 3.7 5.9 3.9 4.7 3.2 3.6 3.3 3.0 4.1 4.0 3.1 4.2 3.7 .3.8 3.9 4.1 3.8 3.0 4.1 3.6 4.4 4.7 4.3 5.2 5.6 3.3 3.8 4.4 4.4 4.1 *Mixed liquorsuspended solids Metro Wastewater Treatment Plant,St Paul,MN