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AR 226 _ 080 ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a componentof FC-206C a1,n1d,2L,-25,43339,.4,4(,15-,O5c,t6a,n6,e7s.u7l,f8o,n8i8c-ahceipdt,adecafluoro-, potassium salf, CAS# 2705-39-3) LR-e5m4a3r9k.s:CurreTnhtei3nfMorpmraotdiuocntiinodniclaottensuimtbisera wmiaxstu5r0e1o.fT0h.e73te%stPFsOamSp,le is 7oc6t.yl00su%lfwaattee,r,1.1457%% dhiyedtrhoyxlyenfeoagmleycro,l1b.ut0y5l%etphoelry,o4xy%etuhryelae,ne1.55% sodium monoocylphenyl ether, 0.06% sodium lauryl sulfate, and 0.05% benzotriazole.. cThhaerafcotlelrowiiznegd csounmcmenatrryataipopnlioefsitmopuarimtiixetsu.reDawtiathmianyconmoptleactceulryately reflect the toxicity of the fluorochemical compnent of the test sample.. wwe. __---- Method: 3M Protocol, Reference EAR 11/8/79 GTeLsPt:tyNpoe: Static acute AYneaalrytCiocmapllmeotneidt:ori1n9g8:1 Dissolved oxygen concentrations Statistical methods: Graphed Test organism source: Activated sludge mixed liquor collected from the Metro Wastewater Treatment Plant, St. Paul, MN Test condition: SDiylnutthieotniwcasteewr:agDee:ionNioztedusweadter. Reference and test solution preparation: Astock solutionofthe test substance was created by diluting 0.60 grams of test sample to 100 mL with dilution water. It is not noted exactly how the 1,000 mg/L test solution is prepared from this stock solution. TNeusmtbveersoseflsc:oncNeonttnroatteidons: 1 plus 1 blank control TToetmaplesrautsupreen:deNdotsonloitdesd and pH on dayoftesting: Not noted Element Basis: Respiration inhibition as determined by oxygen consumption. 001652 RESULTS Nominal concentrations: Blank control and 1,000 mg/L Element values: No acute inhibitory effect on activated sludge respiration rate at 1000 mg/L with a contact timeof 6 minutes. Element values based on nominal concentrations. Rsuebmsatraknsc:e rTeemsatriknsg wfiaelsd.coTnhdeucvtaeldueosnrtehpeormtiexdtuarpeplaystdoetshcartimbiexdtuinrethaentdest not the fluorochemical proportion alone. CThOeNCteLstUsSuIbOstNaSnce exhibited no inhibitory effects at 1000 mg/L over a contact timeof 6 minutes. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kiimisch ranking 3. Testing lacks record of methodology uwsaesd.noTthleonmgeetnhooudgwhtaosdnefoitnaitnivaegleyndceyt-earpmpirnoeivfetdhmeertehmoidg.htTbesetodtuhreartion raedtveenrtsioenetfifmeecst.s tShaatmmpilgehtpuorcitcyurwadusrinnogt ctyhpaircaacltwearsizteedwaatnedrtthreeasttmuednytlacks analytical confirmation of the amountoffiuorochemical proportion in the solution. REFERENCES "This study was conducted by the 3M Environmental Laboratory, St Paul, MN, Lab Request number 68125, 1981. OTHER Last changed: 6/26/00 001653 Co LRN: GBI25 SLUDGE TOX. RESULTS 001654 oi ied Pmh 2 Toreiso)f a Laboratory SludgeHixed i) l Heid To Leo we,are} r05e437 ccsly-2e, 0io I hIl mn sn i n H1T| WNim a Eg re a o i aN o aeahin go li Ni Ii il : ioi g IiHIN nN IN Hi l HL Yo Lia CbsE b IXSc nlm iHnH inFRni egHo ii o Li CEiiHy s2 EfiyoiLii hfliNTavGLhh= LH |IREiiilli}| T Ne :: F HLmS : o Bat EEE = FH HE iN HE Ll TIN i | TEIENNE[EHE3 MN ilill iHil iIr n i G i Bh I wll m lll hFEil i L_--L Acute Toxicity of a In Environmental Laboratory Compound (Substance) ta.Activated Sludge Mixed Liquor Protocol Reference: EAR 11/8/79 Lab request no.: _6F/2 S Date: _2-0/-8/ analyse: 0. Q.So ED A rm ls mr het co 22 era evtenarar aia The activated sludge niedmixed Vliigquuoorr wweass cole collected from the St. Paul/Minneapolis somple description:Lo5439 CCR14-2F FLGEILoT-S0/ oH [eSeie.n[ct |EL Tee Ty el a EA |Dslashe 7TT 1 7] | fselzselze[TT 17 Lo]-Jesolesse TTT 7] (2[= Jepolsae] [TT 3-1 = lees] |_| -- lz 145|2.35 - Sl=l--= tes" 7|1=-1 =1" [ 81 |z2usLe gira TT ||7] = II I I l= IEE A I COMMENTS: BE:ST COPYAvaiapg 001656. "THIS GRAPH WAS MADE By. ComveeTING A WOAXYSGERNEPOPSRIOTBIEONTEODATosTRP(RpODCUaCnEerOVERLo APPINt G cupi ves. 2 & LT ton Ja TH LEp N tic EEE TT TE T Ace eniaf mSon A Joh [Shuses| MLEi ON EER INSEE EA yeeEER WE SE RN E IRo R EH TE Pe LC TT NEN EL tai ordpom 1 TT hE ORN Nw dorlf iFeLN lagLeon LORE kdl ae Ln NL bl LE b r GdnENG EN de AE LSPoinliEINGNGdly Polo EL pd iFlon Ll | afi HE! FE BERET LING EE fbb] fil A ho fer pda ik ali Fh | EN el La tad PL ili Hil i. iE: nb sill Ld? hl lit: pb ef La dl PIETRO Nari To NL BEST COPY AVAILABLE. 7 - Awberes : 001657