Document Vja7KLqk1g0VdwLddbOVO5pX4

HAiyu vol m Auaurr 17 in (lone, whM| ire nlnmlcwd to syrdheein DNA by eootact wfth MUffim. Ml'l mtiat pban until etlmullted 10 rwunter division by ssgnndsry saposure to tbo kim anilisn. By (MMIIm thno would bo iMtnoty ecus. They apparently eemepond to Mlletwa end Miner's "educated T lympho cytes"*, for they wen only obeervbd >1 > tin* when the mitotic response of thymue-dertnd lymphocytes is maitlniel*. We en sumMly seeking diteci experiment*! proofof their identity. rtorioueotMnipnlocetablieh Ibis poor" heve failed, porhlpt bsceuss of the wo of MmmMt entiftiit, which an poor stbMilaton of thynus derived calls'*. Our flnding Implies that cett nplintion may torn as a means of enlaqbii and/or shoring the pool of thimwdetivod cells ospabit of icactlni with certain entlgene. Replication may not, however, be cenettal to tbs helper (unction of than oallt1*. At the peak of the secondary rtepoan to SRC MM0% of the calls In mltoslt In the spleen am thymusuMvad*. Thus we wen Initially surprised to And only %-9% of the spleen cedi responding mitotiottty to a second Injection of antigen wfctt showed evideon of having neponded In the earlier primary response- It It Important to remember that the wash delUwetlnglhs ikne and magnitude of the reepeose of thymus- lerived eeOs to antigen was oarriad out In Irradiated, thymuepraftod mice, Thus It Is possible that (here way be Mot dWtnnen In notmsl mica. If than should be any elgnideam dlWeemes, hotneec. then would probably be an Increase of * Miller, J. J. Ill, /_______ _ ' Mfthr. 1-1 III. end Cede, t MWer, J. J, til, and Cob, L ,_________________ ____ , ! !.C.,MtdFeldnvtn,J D.,y.*w.M^,IJn,J>>(l<iei. Port, C. P., m Trent OrWfciw nw sdU(lea MIL by MhMtn. HjR. and Lautlt, J. r\ |7 (Academia neai, New Yet*. . IW). Polychlorinated Bipbenyb: Photolyila of 2,4,6$ ',4 'fi Hexachlorobipnenyl -___ Matty arctnatlc orgnaochlcrtna ootnpouada an readily utlllaad or othwedn decempotad by a anaabar of apaaMs mitre down. Per naay of thaw compounds ^otorinoderi dagrodadoo, Inducad by tha uhreviolat eoaMat of the gun's liM. mty bt a malor pathway of lit rump ftiWlan. . Bannsa a larga ansnbar of perifcldn anoasdn a tblatnan oh bam shown that tocrsaslng On aussbw of iceoartltiitlag thymut traftt up to a polai Incteataa the number of periphetel thymus derived ceth*. This tnenon don not, however, slier the perocnlepe of ihymuwdertved oelh mpondfng to entleen le lymph nodn nor dose it change the tbnlag of thiir nepoitn. It h ponIMo thet some thymut derived onsnory oedi loet their lebel through divhion e/ter the period of lebeDing. Wo ere taverttpuing the ponlMlity of lecniitmwt of thymuwdsrtved eeih tqr othw thymut derived erth rasponding spedOotlly. The smell number (0-150 of libelled eeOs round in oontral saintsIs may be due. at leatljn part, to tha enwrgsnn aad subteguent raaponsa of a few IhytmW'dsrlmd cede which were undergoini division In the thymus at the tine of thymidine ndaahtiitntipn. aitantkm bn ben paid to the pbotolyils of than eonpounds'-'. Photochemical itsnompotlllon of ddetchlghseyh may ba a tignffitett pracan In tha snvlronutol baeauea day abaorb In that nglon of tha ubmvlolal (nf. and oor unpub lished damonetratlons) which to available In natural condMnne (eonpan nf, I) Aon nmHcpt. Commercially availabla potychlocinlied bipfawiyh (PCB) (or example 'Arodor', consist a( t oomptea mixture of ehlar- hutad Mphenyh which can ba raaotvad iMo non than Ifty peaks by gas chromatofraphy on 'Boot snli--s' (grtwn communication from D. J. Sissom lad 0. M. Tiling). As it is difficult to eorrakie data (Iran susfe a rrmpli r aWnes ws bava eyntbeelnd 1 sarin of bomaric di, tit, tsM, pcMa, lima, octa aid dacachloroblplwnyh to Ibsllllola Ihndciunnl aapwlmant la that ons nauh of tha DNA eynthnts which foUowe primary aosiganlc illimiletloo la tha gaosnlion of Mamory oath. Tha ability to Identify than calk ahould ba of vaiua in ebtrnoMrlilng tbam further. R. K. O. is a raclplnl of a US Publio Haaltb Service Canar Development Award. Tha work waa supported by US Public Health Sendee want horn the NaHooai Qumr Inatinite, the National laaHtina of Allergy end lotariout Dteeaen end en Amerinn Caneer Society sub-gnnl. We thank Kaaunari Hondo and Anna lhapiro (nr technical ewhtanm. New atdW la proof! Since we submitted this manuscript, tnothsr rspoct in which msmocy eaRs wars identified by autortdioersphy, bn appeared. gocmann, C, and Pektman, J. D,, CUx. A?. Iimmmtl., 7, 5dJ; IP70. R. K. OatawoN J. KaOom J. D. NAYwamt B. H. WAKSMMt Drpermwnrr of Peidoihgy and tfitfstabry, Ytk (Mtongy Sdml tf MhHOm. CemmteM 06310 Bassivsd November 33, IP70; revised March 10,1971. 1 Cowsnt, 3. L., end Uhr, 3. W., J. Mrd., IM, 1017 (|M6>. Miller. 1. r. A. P., end MitcheN, 0. P,, TVeruWexr. A/,.. I, 3 inert. ' Devin, A. 1. S., 7Vwuelew. ffrv.. 1,4) onrt. keif. M. C,, MwemTuS. 1337 (l7A). Devin, A. 3. Uuchsn. p. w.ut. v, e-d KcSsr. P. C., OnapleMMM, *, 4)1 (lM). PCB (manuscript In pmptrelion). Ws have abo shown tint all feta 1 OkC nd MM| lpffiqffil Prodwm TLC band I takl PmK 2 Nik) ?mk4 MS M6 Nak 7 . Ml TLC band II Ml M2 MS RataMtottifeM* (rata) 5.6 1.2 9.t I3.S 14.1 11.0 .7 56.9 196 26.1 33.1) mjtotM* -1 222 2S6 290(296) 334030) SSI 906 290 324 391 CbtoriMt tMtaat 0 0 2 3 4.(3*) 3,(4*) 6 2 4 9 6 Conditlonr, msminwot, HawlMt-Packard 3730; cohirm. 4% OF-I+4J4 S30 on 'Chtomcoorb' (AW) 0-M mnh ) . 1/4* outlidt dlsnwtcr, alsn; irtjsclion port Mltpwatun, 320* C; cojumn tsmpotaturo, 173* C; Dante ionnetion betactor temperature and colhcflon port tampsrsturs, 240* C: helium flow rete. 40 wljmm t Prom laotope peek distribution; u, trice (ffydrucerbuii pwm>. M ' not laeaueeo. I COnespondine to atenino tmtariei. Q8?0U HONS NATURb VU(_ 2nd AIMUST 27 lf7l Pig, I 70 aV anaa Omni (DuPoot/CRC 21-110* inetrument). The tampemtuie ofthaaample, controlled ladoaaadonily Don the aouroa Mtuparmtufe14, on 40* C. T7w Inttoilly of Ion comapandtop to the com, ponantt ICU-C,,H.a., a,-C,,H,cr,. and eo00) In Iho inUtura win wfth nmplo temperature. Bock Itomer them u MMO Ion n Iho non Important ` ml. Thin Ion on Indkood n Clx-TO, a,-TO, and oo on. Impact, followed by Ian intann Iona nauUlnp Ikon auonaalvo Ion of Q and/or Ha (maruieoripl In preparation). Ao Iho panaral formula flat PC* la CuHio-.O, the mnletxilar Iona of Individual iaoman an thua nadlly teoo*nlaod la a man apeotmm of a mtnluro of than Compound! (oompan nf. 9). Wo aow aaiah lo raporl tho photoohaadeal behaviour of 2,4A2',4'.6'-he*achlorobipheayl. A aampla of 2S rap ofthle compound In 10 ml. of haaam wn Irradiated al)., 2100 A for IW mhu TNadaym cAnmalopnphy, uafnc `Merck Silica Ool P4Sd* and heaaao, mvaalad Iho pananoa of am major banda; band I (A, 0.40) and band D (Mr 0.6J, ooanapohdiod lo Martini malarial). The man apeotrueo of band I (Tip. I) lodioalod molooular loao oonoopoadlos to dl. tri, Mm, paala and hmaoMoaaMphaaiyb. On liquid ehromatopnphy (OLC) of banda I and II ravoalad olphi and ihno malor pooka reapoctlvaly. Ralaatlon than and man apactra data for all peak oomponaota an roponad m Tabta I. Whaa Imdiallon am earrlad out In methanol, tlmller raaulla wan obtained, but la addition a third and anon polar band appeared, conlalnino oxypenatad polycblorlnatod compound! with attuctuna which have not boon Inveetlpatad Author. Two oonduaiona can be drawn Ikom tho rveutta of thla atudy: (I) durlap photolyala, chloaina la removed ralhir readily from haaaohloroblphanyl yltlitlni compormda oflower chlorine eontonl; (h) photocharatoal production of new compound! format by Ion of chlorine, reonanpamanl or oondanaatlon may lead to furthar oompllootinwa la realduo aaalyaia with tho poaalblo appaaronm of chiorobiphaeyla ("peeka") la onvlronmantal aampln that am not proaaat hr oommwclal PCB mlxturee, S. Sam O. Htrraieoia Atiooik Atfltml t olororory,. Mufcnaf Aawart* Cornell of Canada, HtOfOx, Mate Scotio Raaalvad March 22.1*71. ^SSToPdoI" * Crojbj^D. O., and Tan|. C. 1. Atrk. Food Chtm., 17, 1041 ' Moehr, A. R., Ouanti, W. D,, and Millar, L. L., Sdtntt, 104, IMKIMM. * PNanmor, I., kllmahlal. U. I,, ond Hummer, B. B., Schott, 147, 67 (UM) ' Millar. L L, and Nareap. R. S., Sekott, 14*. M4 (1970). Friedel, R* and Orcbie, M., VUVSooo*etroofArormde t (WHor, NowYotiTleSI). ' Hutahgrr, O-Juileooa, W. D,, and Ztto, V., Mann, 22R d4t 14 Jandnaa, W. D, and Maaoa, P. O.. Aar. Sef. Ihanaai., dl, 7)0 (1970). Selection for Resistance to Carbamate and Organophoephorus Insecticides in Anopheltt *mmmm Tim devatopmant of neMaaoa In aaophaHaa moapaHom la DDT, HCH, and cydodlaoee, and the comanuoat aaa hack la malaria aradketlon pmaraimnaa boa loot urgency m Iho aoaaat far alternative inaootMdm lo replaoo Iho tihloilaiiid hydbo- ` Durinp the paat taw yean, amm than IJtt new bean taatad under an intoanaliorral oolabtranlhia | iho World Health Oitanbatkm1. Taatlaf eaaaWa af a in ah atapaa are reootnmendad for uaa oa a larpa acale. Owe of the Inamtlrldm that aatiaAed thoaailarihof atapa V] (opaamtloaal evaluation) hnd hao been found ardtabh tea ataao vn (apldamloloploal evaluation) la the narbaaaata, pmpaaueV. It ialntritiaioaly mam toxic to aduha ofmany moamtheapaaim than dMdria, enalathion, feathlon and fiolirelhtaa. and aa a raaMual apray haa boon found to ba aSkcliva hr 2 to 4 atantha againtt AoofMtt immUm and A. fmatm la AMaa, A. mphaaafand d.drtaOinIraa,andd.aMamtNalaBllalmdaH. No cam of realatanoa to carbamate or orsoaophoapheava intactIcidm haa yet been reported althouSk lofcaaaiee to makthton hoa boon euapoctod la A aMmaaaa In Owatomala and Nicaiepua*'4 and rubaadnaatly daaaoaatretad la Salvador14. In thia oomeaunicatioa wa report tha devatopaant of high deprooa ofcatbamato and organophoaphorue lealataaca in A. altlMonu reeulting from iatanaive rejection paeaaure in tha Acid, and aubaaquently la tho laboratory. Earlier attempt! to Induce oarbotaalo tealatanm la A. dUmama by laboratory actedion have boon unouaaaaaAd, although in Colt* pfrUot foUfoiu a 30 x level of tralalanm to propoaur developed In larvae within twentydive gaaotatleoa ofaolootionpreaauie''*. A attain of A. otbtmmui from Paaaam elected with nMaopropylphonyl mothylcarbamaia Am Afty panareliom developed only a 2.1 Inctoaaa la the LCae leva* Another attain from Halil, oootalnlnp dioldriiwvelotaal Indict duela, when aubjoctod to larval aalaction praaaum by; for nineteen paoarattona end to aduk preaet MOMS 0 8 7 0 1 3