Document VjLm0orjbx5VL64xKo1Q02O6w

ARL26 - 0802 ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE. Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a componentof FC-206C and 81-2/8. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,58,h6ep,t6ad,e7ca,f7iu,o8ro,-8, potassium salt, CAS # 2795-39-3) Remarks: The 3M production lot number was not noted. The test sample is FC-206C and is referredtoby the testing laboratory as "81-2/8". Current information indicates it is a mixture of 0.73% PFOS, 76.09% water, 15% diethylene glycol butyl ether, 4% urea, 1.55% sodium octyl sulfate, 1.47% hydroxy foamer, 1.06% polyoxyethylene monooctylphenyl ether, 0.06% sodium lauryl sulfate, and 0.05% benzotriazole. The following summary applies to a mixture with incompletely characterized concentration of impurities. Data may not accurately reflect the toxicityofthe fluorochemical compnent of the test sample... METHOD: Method: EG&G, Bionomics protocol entitied "Methodsfor conducting flow-through toxicity tests with freshwater fish". Test type: Flow-through acute GLP: No Year Completed: 1981 Species: Pimephales promelas `Supplier: Commercial supplier in Nebraska Analytical monitoring: Temperature, pH, DO, and conductivity Exposure period: 96-hours. `Statistical methods: LCs values calculated using a computer program (created by Stephan, 1978) based on the moving average angle analysis, probit analysis, or binomial probability as appropriate. Test fish age: Not noted. Length and weight: Loading: 0.38 g/L. Length = 33 -- 54 mm with a meanof 41 mm Weight = 0.25 -- 1.53 g with a meanof0.57 g Pretreatment: Not noted. Test conditions: - Dilution water: Well water supplemented with Townof Wareham untreated and unchlorinated well water. Dilution water chemistry: 001658 AHlakradlinneistsy:: 2128--2244 mmgg//LL ((aass CCaaCCOOss)) Conductivity: 90 pmhos/cm Lighting: CoPolHW:hite fluorescent6.li7g-ht6s.p8rovided a daily photoperiod of S1t6ohcokurasndligthetsatnsdol8uthioounrsprdeaprakrwaittihona:n iAntpernisimtayryofs3to0c--k 7so0luftoiootncoafnd6l8es1. wmagt/emrLanwdasdiplurteipnagrteod4b0y00admdLi.ngAl2l7t2e4stgsroalmustioofnstewsetrseamprpelpeatroeddibstyilaled diluter control delivering water. five nominal concentrations of the test sample and Flow-through rate: 5.9 aquarium volume replacements per 24 hour Sptearbioidl.ityofthe test chemical solution: Not noted. Exposure vessels: 39 x 20 solution volume of 15 ters. x 25cmglass tanks which maintained test Number of replicates: two NNuummbbeerroofffciosnhcepnetrrraetpiloincsat:e:5 p1l0us a blank control Water chemistry during the study: pH range (0-96 hours): 6.87.1 (control exposure) Temper6a.t7u~r7e.3ra(n2g0e00(0m-9g6 hoeuxrpso)s:ure) 21 -- 22C (for all aquaria) Dissolved oxygen range (0-96 hours): 7.7-9.0 mg/L (control exposure) 7.1-8.7 mg/L (2000 mg/L exposure) RESULTS Nominal moll concentrations: Bk control, 125, 250, 500, 1000, and 2000 Element value: 96-hour LCso = >2,000 mg/L. Element value based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the test snoutbstthaenfcleuorreomcahrekmsicfaielldp.roTpohretivoanlualeorneep.ortedappliesto that mixture and CONCLUSIONS - 001659 Tbehe>2F,C0-0200m6gC/L9,6-thhoeuhrigLhCessotfcoronPciemnetprahtailoenstpesrtoemde.laNsowmaosrtdaleitteyrwmainsed to observed at this concentration. `PSauubl,miMtitenrn:es3otMa,Co5m5p1a3n3y, Environmental Laboratory, P.O. Box 33331, St. oataQuAUTY Rteesltiianbgi.litHyo:weKvieirm,istchherraeniksinngo 2i.nfTorhmiastsitonudtyomceoenftisrmallthtehediclruitteerriwaafsor quality wsotrudkyinlgacpkrsopaenrallyy.ticSaalmcpolnfeiprumraittiyownoafstnhoetapmroopuenrtloyfcfhlaruaocrtoecrhiezmeidcaalnd the proportion in solution. REFERENCES _ TMhaisssascthuudsyewtatss catontdhuecrteeqduebsytoEfGt&hGe,3BMioCnoommpiacnsoyf, W3aMreLhabamR,equest number 7038, 1981. OTHER Last changed: 6/26/00 001660 : . EAL# 812 & LRA 703% ACUTE TOXICITY OF 81-2/8 TO Fc-206 c FATHEAD MINNOW (Pimephales promelas). SeRueETE HAE nga igen 50 FR 3M ---- REPORT. $BW-81-10-1029 Vazenan, eHnassBoboLmsets Aquati* c EGT&oGx,icoBiloongoymiLcasboratory October, 1981 -- 001661 INTRODUCTION The purpose of this study was to estimate the acute toxicity of 81-2/8 to fathead minnow (Pimephales promelas) under flow-through conditions. Preliminary-range-finding static tests and a subsequent 96-hour flow-through test were conducted at the Aquatic Toxicology Laboratory of EG&G, Bionomics, Wareham, Massachusetts. The acute toxicity of 81-2/8 was measured by estimating the LC50 value for the compound at each 24 hour interval of the test. The IC50 is defined as the concentration of the test compound in dilution water which caused mortality of 50% of the test -animal population at the stated exposure interval. The flow-through test was conducted from 24-28 September 1981 with a two day pre-exposure period. All Taw data generated are stored at `the-above location. MATERIALS AND METHODS Procedures used in this acute toxicity test closely followed those described in the EG&G, Bionomics protocol entitled "Methods | for conducting flow-through toxicity tests with freshwater fish." Values are reported to different levels of significancedepending on the accuracy of the measuring devices involved in any one process. ' The 81-2/8, an amber colored liquid, was received from 3M, St. Paul, Minnesota on 12 August 1981. The test material was stored in the dark at ambient temperature (21C). The material was tested as 1008 i 001662 : 2 active ingredient. Nominal test concentrations are reported as milligrams of 81-2/8 per liter of test solution (mg/). The fathead minnow (Bionomics lot $81A17) were obtained from a `commercial fish supplier in Nebraska and held in a 500-2 fiberglass tank under a photoperiod of 16 hours light and 8 hours darkness. , ALL fish vers fed a dry, pelleted food, ad libitum, daily except during the 48 hours prior to testing. There was 1.0% mortality in the test fish population during this 2 day period (Daily Record of Fish Holding Conditions). The well water which flowed into this tank was characterized as having total hardness and alkalinity ranges as calcium carbonate (CacO,) of 22-24 mg/t and 18-21 mg/2, respectively and a specific conductance `range of 80-90 micromhos per centimeter (umhos/cm) (Weekly Gravity Feed Tank Water Quality Analysis Logbook). Other parameters monitored in the holding tank were a pi range of 6.5-6.7, a dissolved oxygen (DO) range of 94-99% , of saturation and a flow rate of 18 tank volume replacements/day : (Weekly Record of Fish Holding Water Characteristics). Test fish were maintained under these conditions for a minimum of 14 days. The temperature in the holding tank was 21C during this 14 day period (Daily Record of Fish Holding Conditions). The specific conductance was measured with a YSI Model #33 salinity-conductivity- temperature meter and probe, the pH was measured with an =e Instrumentation Laboratory Model #175 pH meter and combination ~~ . electrode, the DO was measured with a YSI Model #57 dissolved 001663 . 3 oxygen meter and probe and the temperature was measured with a Brooklyn alcohol thermometer.- Total hardness and alkalinity were measured according to APHA et al. (1975). : The exposure system used in this study was a modified, proportional | diluter similar to that described by Mount and Brungs (1967) with 2 0.50 dilution factor. The dilution water was well water which | was pumped to an epoxy coated concrete resefvoir where it was supplemented with Town of Wareham untreated and unchlorinated well vater and aerated. This water was characterized as having total hardness and alkalinity ranges as CacO, of 22-24 mg/s and 18-24 mg/L, respectively, a pi range of 6.7-6.8 and a specific conductance Of 90 ymhos/cm (Weekly Gravity Feed Tank Water Quality Analysis Zogbook) . i The diluter delivered five nominal concentrations of 81-2/8, 2000, 1000, 500, 250, 125 mg/2 and control water containing no | 81-2/8 to duplicate test aquaria. Each glass test aquarium measured | 39 x 20 x 25 centimeters (cm) with 19.5 cm high drains which maintained water volumes of 15 . The diluter delivered 0.5 % of test water to each aquarium at an average rate of 178 cycles per Gay. This is equivalent to 5.9 aquarium volume replacements per : 24 hour period. Illumination was provided by Cool White fluores- I cent lights centrally located above the test aquaria. Sixteen hours of light at 30-70 footcandles at the water surface were 001664 : 4 provided each day. Test temperatures were controlled by immersion coil heaters in a water bath where the test aquaria were located. ) A 4-2 glass Mariotte bottle in conjunction with a "dipping bird" | delivery system (Lemke, et al. 1978) was utilized to deliver a stock solution to the mixing chamber of the diluter with every I cycle. The stock solution of 681 mg/mi was prepared by adding 2724 grams of 81-2/8 to distilled water and diluting to 4000 mi. + The 81-2/8 stock solution was introduced into the diluter and ' cycling began on 22 September 1981. The test was initiated two days later when tenfatheadminnow Vith'a mean (range, Ne30) vet weight and total length of 0.57(0.25-1.53) grams and 41(33-54) } millimeters (Fish Weights and Lengths Log) were randomly distri- | buted to each aguarium. os | Biological observations of the fish and observations of the | physical characteristics of the test solutions were made and recorded at 0, 24, 48, 72 and 96 hours. The pH, temperature and dissolved oxygen (DO) concentrations were measured in both repli- cates of the control, high, middle and low concentrations at 24, 48, 72 and 96 hours and in both replicates of all concentra tions at 0 hour of exposure. 001665 : 5 The concentrations tested and the corresponding mortality data derived from the toxicity test were used to estimate 24-, 48-, 72- and 96-hour median lethal concentrations (LC50) and 95% confidence intervals. The computer program utilized (Stephan, "1978, personal communication) estimated LC50 values using one of three statistical methods in the following order of preference: moving average angle analysis, probit analysis, binomial probability. The method selected was determined by the characteristics of the data base (i.e. presence or absence of test concentrations causing mortality of 100% of the test animal population, test concen trations causing mortality of a partial number of animals in the population, etc.). The computer program scanned the data base, identified the most preferred statistical method and performed the analysis. ; RESULTS - ! The test material 81-2/8, was not acutely toxic to fathead minnows i at nominal concentrations as high as 2000 mg/% (Table 1). Al- hough sporadic mortality and lethargy of the fish were observed, there were.no dose-response trends suggestive of 81-2/8 related effects. In addition, the control mortality incurred suggest that the mortality and lethargy observed were due to extraneous -- . facandtnoot 8r1-2s /8. The estimated LCSO values after 24, 48, 72 and 96 hours of exposurewere>2000mg/g, `the highest concen- tration tested. 001666 : 6 The temperature in various aquaria ranged from 21-22C during exposure. The pH and dissolved oxygen concentrations measured during the toxicity test are presented in Table 2. 001667 LITERATURE CITED APHA, AWWA, WECF. 1975. Standard methods for the examination Of water and wastewater. 14th Edition, Washington, D.C. 1193 pp. 4 | Lemke, A.E., W.A. Brungs and B.J. Halligan. 1978. Manual for construction and operation of toxicity-testing proportional ! diluters. EPA-600/3-78-072. Mount, D.I. and W.A. Brungs. 1967. A simplified dosing apparatus for fish toxicology studies. Water Res. 1: 20-29. Stephan, Charles. 1978. U.S. EPA, Environmental Research Labor- atory, Duluth, Minnesota. Personal communication. i - i 001668 `Table 1. Concentrations tested, correspondingpercentage mortalities and behavioral observations of fathead minnow (Pimephales promelas) exposed to 81-2/8 under flow-through conditions. Twenty fish were exposed to each concentration. dy (mg/2) concentration ee Row 4P8erhcoeunrtage 7% how mortality 36Four ree - 2000 1000 500 250 125 control 0? - 0? 0 0? 0 cot 0 02 0 0 [ [ 02rd 0? 0? 1520 52 15 orb 52 0 15 5 15 -= . - Suds formed on the surface of the test solution. - Psome ish were lethargic. : one fish was lethargic. 001669 | Table 2. The pi 4nd DO concentrations measured during the 36-hous: Elov-theough toxicity tant vith fathead mimnov (pissghales promelas) and 81-2/0. The range of messurements made from replicate aquaria are reported. ow ween coownngtnniaon 2000 10s0000 250 con1tr2o5l 20 10s0000 ponen12t2550 223 woimeasuropent made. ; 3 oho na a7l.2a 7.1 p7.n1 ness w8.s6 8.s90o-s.9.0 anew rama ne--na J. 10"7.0 neo as-o2a anam=mee whew Tahu 9 hour es eas 6s er-es e-r2 ea#s : 2 A - sass 6.6-6.7 sre 6.8-6.9 | o6.9 o eso nae 7a r-e iteara raas naw=smo e1 tse-s*nas e1=ss ' 7 SUBMITTED BY: Aquatic TEGoSxGi,colBoiognyomLiacbsoratory Ware7h9a0m,MaMianssSatcrheuestetts September, 1981 PRINCIPAL INVESTIGATOR: Brenda F. Wilson Aquatic Biologist STUDY DIRECTOR: DATA AUDITED BY: Gerald A. LeBlan faatic Toxdcologist - Robert E. Bentley Director, Quality Cr nies 001671