Document Vj4e0Kg1eBaZjy70Mex9xYoqo

A R ^ - o s ii MICROTOX TOXICITY TEST TEST SUBSTANCE Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, FC116, FC-126, FC-169, orFC-143. (Octanoic acid, pentadecafluoro-, ammonium salt, CAS # 3825-26-1 ) Remarks: 3M production lot number 390. The test sample is FC-126, a white powdery solid. It's purity was not sufficiently characterized, though current information indicates it is a mixture of 78 - 93% test substance and 7 - 22% C5, C6, and C7perfluoro analogue compounds. METHOD: Method: Beckman's Microtox "BASIC" Procedure Test type: Static acute GLP: No Year Completed: 1987 Species: Photobacterium phosphoreum Analytical monitoring: Light loss. Exposure period: 30-minutes Statistical methods: Not noted. Test organism source: Not noted. Test conditions: Diluent: 2% NaCI Microbic's Reagent Deionized water pH: 6.5 Stock and test solution preparation: A 2000 mg/L stock solution was prepared by dissolving 200 mg of the test solution in 100 mL diluent. Test solutions were prepared using aliquots of the stock solution. Concentrations dosing rate: Once Stability o f the test chemical solutions: Not noted Exposure vessels: Cuvettes. Number o f replicates: two Number o f concentrations: Four plus a blank control RESULTS Nominal concentrations: Blank control, 420, 560, 750, and 1000 mg/L Element value: 5-minute EC20 = 810 mg/L 5-minute EC50 = >1000 mg/L 5-minute ECso = >1000 mg/L 15-minute EC20 = 520 mg/L 15-minute EC50 = >1000 mg/L 15-minute ECso = >1000 mg/L C03861 30-minute EC20 = 420 mg/L 30-minute EC50 = 870 (810-930) mg/L 30-minute ECso = >1000 mg/L All element values based on nominal concentrations. CONCLUSIONS The FC-126 30-minute EC50for Photobacterium phosphoreum was determined to be 870 mg/L with a 95% Confidence Interval of 810 to 930 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 2. Testing meets all criteria for quality testing, but lacks analytical confirmation of test substance concentrations. There is a lack of characterization of the test sample. REFERENCES This study was conducted by the 3M Environmental Laboratory, St. Paul, Minnesota, Lab Request number E1282. OTHER Last changed: 5/24/00 003862 ENVIRONMENTAL LABORATORY BECKMAN'S MICROTOXR SYSTEM MICROTOXR RAV DATA LRE12uSi l <4 ^ TEST SOBSTANCEi F C ' I Z t n L o 'f 3 9 0 ____________________________ ________ TEST DATE: __________________ AMAlTSTi C \ . {JLIX jcL l__________ SAMPLE CUVETTE B1 Cl PINAL ASSAY CONCENTRATION rv\a f L. BLANK CONTROL DUPLICATE ITFTSI --------- FTSXTTEEXBIHsS READING 5-MIN. 15-MIN. 30-MIN KO) 1(5) 1(15) K30) 9 0 s\ 2 5 97 s s . 2b 2b 2 6 " B2 9 A O 93 2 3 76 6 1 C2 Dopli'c<vK 99 9 0 7H B3 &ls> O 9S~ 2 3 75 66 C3 b>o plfcdLAe Jo a 5 0 75 71 B4 7SO IO [ 2 3 6 1 S9 C4 t> u p |ica.i 99 2 0 Jo7 sg B5 Joo 95- 6 91 39 C5 bpli'x+ i 96 70 n 3 Terf Sulostanc (u;|r\ite. p>cacler^ t>< \o e n 4 e S-+ccVi e \r\- A o o ji&c> J m i - p. +o cKss.cWe. cow\iple4ely P rt= v5*7 + o >>7 iuH-V\ dU\c4c. AicvCW % l^ C -L -M icrcW s ^ A ^e n f I n f i l l b>ilu4lens,* D U UJa-kr USci ulturlt T^o/lo) >yj t i l m u M k roujs P-o w 'j 8 * c j. t>ii4'"*<* H-O-S7 pH- ,,S' ^ ^C'0Ok30<8 6 3 BECKMAN'S MICROTOXR SYSTEM MICROTOXR DATA REDUCTION LR__ 13. f i A Datp Analyst-- BLANK RATIOS* - Final Initial PC-lsa L c -i ----- 5=Fmr T5=HIir ------ 3=HHT------ BLANK CUVETTE BLANK RATIO DIFFERENCE BLANK RATIO DIFFERENCE DIFFERENCE B1 Cl Vsg*o..q7,7 O -O I o .o r,K I'A ' o.^LI Ls/J& ',..ut? o . oo\ *The blank ration for B1 and Cl should agree within 0.02. MEAN BLANK RATIOS Bl BK RATI,O +- Cl BK RATIO 0.183 30-MTN. _______ Q- *1 3 ____ _ _____ 0 , <?h i______ NORMALIZED LIGHT LOSJ /nBin/\ u ErrECTJ _ Mean Blank Ratio x Initial Saaple Reading Final Sample Reading ~1 SAMPLE CUVETTE Test Concentration g C3 S b O 7SO " 25 lOOC> 5-MIN. r o lo \ O 03 l .iV\S~ o it H o .M 4a c - Ac H o . HidT o. 15-MIN. P o . Ro 3 O ./g 7 <?. fcA O -458 J . 1 Cfe O S Ak 30-MIN. P 0 .3 o 3 0 .M H O. 3 0 .(0 S*f 0.1,31 1 - Hi l.<M3 Z LIGHT LOSS (XA ) - -- C-- X 100 i+r SAMPLE CUVETTE Test Concentration 5-MIN. X A 15-MIN. ZA 17 30-MIN. ZA 33 5 7-S ' li *3 :3 S b D b4 :4 7 5 0 35 . :s l o o o > (i to !(* 17 *1 ____ 1 *___________ Al Al 31 JA <1 S IS Ad AS H1 11 EFFECTIVE CONCENTRATIONS (EC's)____________ (Calculated using T.C" data pairs.) EC's , Ai\ \N 5-MIN. . 20 1 0 s--Cr-T-T---- fu-'"_r>n0 -, 1 ` ^ x 80 ( l U o - l ^ ) &SO = 34UO 15-MIN. /S - M ia/ ~ SdsO . & c - 1 JOO (lt>5o-- II5 0 ) BCao - J S 3 0 30-MIN. 4 *o S 7 0 ('s 1 0 - 4 3 0 ) " (;*u *iS 6 4 ' / lo o o