Document Vj4BkNoomb0NqQ3yrr4o6QReg
AR 886 -- 077%
TOXICITY TO AQUATIC PLANTS IESTsuBSTANCE
Identity: MreifxetrurreedctoontaasinPiFnOgSp,erFfCl-u9o5r,ooocrtaansesauclfoomnpaotenewnhticohf mFCa-y2a0l3sFoCb.e (1Octanesulfonic acid) (CAS # 2795-39-3).
RiseFmCa-r2k0s3:FC_,Tihdeent3ifMiepdrboydutchteiolnablootrantuomrbyearswRa1s33n.ot nCoutrerde.ntTihnefotremsattisoanmple
i7n4d.i4ca6t%eswaittiesr,a 2m.i3xt4u%reaocrfyl0i.c8f5o%amPeFr,OS1,.71%7a%lkdyileetthhyelrenseulgflaytceo,l2b.u5ty5l%esthoedri,um
otcrtiyelthsaunlofaltaem,in0e.,1a2n%ds0o.d0i4u%m
tnoitlryiltter,ia0z.o0le9.%
sodium -
molybdate,
0.85%
Tchhaerafcotlelroiwiznegd scounmcmeantrryataipopnliseofsitmopuarmiitxiteusr.e Dwaittha imnacyomnpoltetaeclcyurately reflect toxicity of the fluorochemical component of the test sample.
METHOD:
MTyepteh:odA:cuOtEeCStDatGiucideline 201
GLP: Year
sNtuody
performed:
1997
Species: Source:
OSriegliennalalsytorbutmacinaepdrifcroommuAtTuCmC,
MD,
maintained
at
AScl-DETD,
Duluth, MN. Element basis:
Algal
cell
counts
(cells/ml),
specific
growth
rates.
EStxaptoisstuircael pMeertihodo:ds9:6 Shtoautirsstical analyses were performed using the Trimmed
ASnpaelayrtmiacnal-Kmaornbietrormientgh:odp.H, conductivity, and temperature
Test Conditions: AplrgoavlidNeudtarlilemnitneMreadlinuumtr:ieNnuttsrieesnstenmteiadlifuomr aplegralOgErCoDw.ih Tahnids anlustroisenetrvmeeddaisumthe diluent for all algal operations. The pHofthis synthetic algal medium was 8`wS0at.socpkraenpadretedstbysodliustsoilovninpgre7p0a0ramtgioFn:C-A20230F0C0imng3/5L0pmriLmaarlgyasltmoeckdiSuoml,ution. _ fvoollluomweedadbdyitmieocnhaofnitchealstsoticrkritnog.alEgaalchmetdeistumco.ncentration was made by Esoxlputoisoun,resevaelsesdelwist:h 2fo5a0mmlstEorppleernsm.eyer flasks containing 100 mi test Agitation: 100 rpm.
001368
Number of replicates: 3 for exposure concentrations, 6 for blank control. INnuitmiablecrelolflcooandcienngt:ra1t.i0oxns1:0* fcievlelpsl/umsLa(1b.l3axnk1c0o*nctreollls/mL actual). Lighting: Continuous lighting at 740 ft-candles using cool-white fluorescent lamps. Water CphHemriasntgrey(:0-96 hours):
8.00 - 9.90 (control exposure) 5.20 --7.83 (1000 mg/L exposure) Conductivity range (0-96 hours): 151 -- 186 pmhos/em (control exposure) 194 -- 205 pmhos/cm (1000 mg/L exposure) Test temperature range (0-96 hours): 24.8258 C (incubator)
Reswts Nominal concentrations: Blank control, 130, 216, 360, 600, and 1000 mg/L.
Algal Growth Response ECs, Values
Exposure (Contact)
Cell-Count
Hours
mglL (95% C.1
[2a| 208(207--428)
<130 (Cl. not calculable)
[86 <<113300 ((CC..I._ nnoott ccaallccuullaabbllee) |
`AvGerroawgtehSpReactiefic mglL (95% C.1) 187 (168 -- 209) 271 (239 = 308
Element values: 9966--hhoouurr EECCssoo==<217310(m2g3/9L.~ (3c0e8ll)cmogu/ntL) (growth rate)
96-hour NOEC = 17 mg/L (cell count) 96-hour NOEC = 57 mg/L (growth rate)
Element values are based on nominal concentrations. wReevreersdiibliutleidtywiotfh GarlgoawltmheIdnihuibmitainodn:cAullitquuroetdsfoorf atnhead1d0i0t0iomnalgpetreisotd.solBuatsioend on growth observed in the recovery phase, the effect on algal growth was. found to be algistatic.
RSuebmsatraknsc:eTReesmtianrgkwsafsielcdo. ndTuhcetevadlounestrheepmoirxtteudraepapslydetosctrhiabtemdixitnutrhee aTnedstnot
the fluorochemical proportion alone.
001369
conclusions
FC-203FC hour E,Cso
exhibits a 96-hour ECso growth rate value of 271
cell count value of (239 -- 308) mg/L.
<130
mg/L
and
a
96-
Tcohuent96a-nhdou5r7Nmo gObsfeorrvgerdowEtfhfercatteC.onTcheinsttreasttisounb(sNtaOnEcCe)wiass17demtgelrLmifnoerdcetlol be algistatic.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATAQUALTY
tReesltiianbgi.litHyo:weKvieirm,isschamrpalnekipnugri=ty2.waTshinsotstpurdoypemreleytcshaarllacttheercirzietderiaanfdorthqeuasltiutydy lacks analytical confirmation of the amount of fluorochemical proportion in the solution.
REFERENCES
TDhiviissitoens,t wDualsutcho,nMduNctaetdthbeyrAeSqculesCtoropfotrhaeti3oMn,CDoumlputahnyE,nvLiarobnRmeenqtuaelstTensutminbger R1335, 1997.
oTHER
Last changed: 6/26/00
001370
EAnSveilrCoonmrepnoarltoTns/iAnSgelDiDvuilustohn
.
"ASel Study 101 5010068
STUDYTITLE
GROWTH INHIBITION OF R1335 FOR GREEN ALGA (Selenasirum capricornutum)
DARD
a
OECD GUIDELINE 201
-
STUDYCOMPLETED
April 7, 1997
TESTINGFACILITY
AScl Corporation/AScl-Duluth Environmental Testing Division
4444 Airpark Boulevard Duluth, MN 55811
Tel. No. (218) 722-4040 Fax No. (218) 722-2592
STUDYIDENTIFICATIONNUMBERS
AScl Study IDA 5010-068 3M Company Lab Request R1335
SSppoonnssoorr:Su3dMyC[oDmFpaRInSyIS
1
001371
EAnSvcilrConomrepnotraaltToens/tiAnSgcDDiuvliustohn ase Suey IDF 5010068
1.0 GENERAL INFORMATION
Study Title
`Sponsor
sponsor's Representative
l
i Sponsor'sLabRequest
"Testing Facility
Prova-- Principal Investigator
[| Testing Facility Director
Growth Inhibition of R135 for Green Alga
(Selenastrum capricornunum).
OECD Guideline 201.
S3eMrviEcnevsi,roBnuimlednitnagl 2T-e3cEh-n0o9l,o9g3y5anBdusShafAevteynue, 1
St. Paul, MN 55133; Tel No. (612) 778-7452.
`Susan Beach, 3M Environmental Technology |
aBnudshSaAfveetnyuSee,rvSitc.esP,auBlu,ilMdiNng525-133E3-;09, 935 {
Tel No. (612) 778-7452.
ss | "AScl Corporation/AScl-Duluth Environmental |
Testing Division, 4444 Airpark Boulevard,
Duluth, MN 55811; Tel. No. (218)722-4040. |
Jo[oaomesomcue| Donald Mount
|
Data Validation and Report Review
RetentionofRaw Data and Final Report
{ CocationofRaw Data and Final Report
l
`Alan Mozol
ya Report Signature; |
"Two years from the date study is completed. |
"ASel Corporation/AScl-Duluth Environmental |
Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040.
SSppoonnssoorr:SIiMdCToDmWpRaInZySS
2
/
. 001372
EAnSvcitroCnomrepnotraaltiToens/tAinSgelDDiuvliustohn 'ASCl Study IDF 010.068
2.0 OBJECTIVE(S)
c"Tooncdeentterramtiinoen t(hNeO4E-Cd)meodfitahne etefsftecstubcsotnacnecnet,raRti1o3n5(,ECfosr)garneednthaelg4a-(dSneloeonbassetrrvuanbclaeperfifceocrtnutum) 1 under static test conditions.
This study was conducted according to OECD Guideline 201 (OECD 1993).
|
Test Substance
I 1 Test Organisms
i
IExposure Chambers
3.0 TEST METHODS
"Properties: R1335, clear amber liquid, sable, and miscible with water. |
T1e0s0t0comngc/eLn.traAtiosntso:ck
0 (algal solution
mweadsiuprmepcaornetdrob)y,
130, mass
216, 360, 600, and addition to algal
medium made by
followed by mechanical volume addition of the
stirring. stock to
Each test concentration algal medium.
was
-
| Source: ATCC 22662
"
Inoculum preparation: recommended medium
Algal stock was subcultured in for 4 days before test initiation.
OECD
"
oUbsteaiinn atensti:nitSiatloccekllcudletnusrietywaosf d1i.l0utXed1a0n%dceslpliskpederinmtlo (t1es.t3 sXolu1t0i%onascttuoal | concentration). "NPare,pEaDraTtAio)n:toDOeiEoCniDzerdecwoatmemrenwdaesdspcoinkceedntwriatthionnust.rieTnthestmoocskst (rienccleudnintg: | chemical analysis for deionized water is in Raw Data Package.
Characteristics: At test
0.22 ym filtration prior
time,
to use
the
in
medium
the test.
had
a
pH
of
8.00.
Sterilized via
Type: Sterilized via autoclave 250-ml borosilicate-glass Erlenmeyer flasks
sealed with foam stoppers.
rUespeliicnatteessta:nd10th0remeiroefplailcgaatlesmpeedrituemst osrubtsetstanscoeluctoinocne/ncthraamtbieorn.weSriex contr+:ol | employed. During the test, kept stoppered, except when experimental. observations were made.
SSppoonnssoorr:SItMdICoDmpaRn2y.25
3
003373
EAmsicroCnomrepnotrasiToens/tAiSnegl-DDivuilsuiionn "ASl Sudy 104 2010068
`Incubation
Observations C`aElncduploaitnitons
Duration: 4 days.
So I
Dfaluiolryespcheonttoplearmiposd.: TCeosnttcinhuaomubserast 7he4l0d foin-csanhdalkeesrutsaibnlge sceotola-tw1h0it0erpm. |
| Temperawre: 25.0 + 2C. `IBmiporloogviecadl:NeDuabialvyercelhlemcaocuyntlsomuestienrg.direct microscopic enumeration via |
Water chemistry: and temperature,
((2)1)dAaitlyt-estteimnpietriaattiuorne.andAltlerdmeitneartmiionna-tipoHn,s
conductivity, were made.
|
according to APHA methods (1995).
a1-v,er2a-,ge3-s,peacinfdic4-g0roEwCtshy'rsa,teasn(dx)4.-4SNtaOtiEstCicablaasneadlyosnesboptehrfcoelrlmecdouunstisangnd
Trimmed Spearman-Karber Method (ECso's) and ICp (Cy).
2
4.0 RESULTS
[Test Substance Toxicity (Pertinent data are in Tables 1, 2, and 3)
| { |
| 1Cel1ldCoEuCnytg:1-d18E7Csmyg:/L29(186m8-g2/09L).(207-428): |
C1el2ldCoEuCnss:2-d13E8Cmsg:/L<(1N3o0t Rmegl/iLab.le). + I
1Cel3ldCoEuCnst;3-d16E3Csmpg:/L<(113450-1m8g4)/.L. vao |
Cell Count 4d ECs: <130 mg/L. 1 4-d ECsgi 271 mg/L (239-308).
1 =
Cell Count 4-d NOEC (ICI0): 17 mg/L
ar-18). 1 4d NOEC (IC10):
57 mg/L (53-62).
1
Effect was determined to be recovery of cells previously
algistatic based exposed to the
on
|
1000 mg/L test substance concentration: ~~ _|
SSppoonnssoorrs Se3udMy CIaDmFpRasIyS
4
a
001374
EAnSveilrConomrepnotraaltiToenst/iAnSgchDiDvuilsuotnh "ASel Sud IDA 5010068
`Test Conditions
Temperature ("C): 24.8258.
Light intensityfi-cdls: 740.
3
PH: 5.20990.
Specific conductivit
os/cm): 149-205.
5.0 TEST QA/QC
QAIQC Critcia
EE
Alelagsatlacofaucnttosr ionfc1on6tdruolrimnugsttheinfcirrsetas3e-0byofatthe | cDouurnitnsgitnhcerefaisrsetd3b-yd aoffatchteorteostfcaopnptrrooxliamlagtaell"y |
exposure.
157.
Test duration must be 4.
[ew esda omion st}
6.0 REFERENCES
[American Public Health Association (APHA). 1995. Standard Methods for the Examination of | `Water and Wastewater. APHA, Washington, D.C.
{| TOersgtainnigzaotfioCnhefmoircEaclos.noOmEicCDCooPpuebrliactaitoinonanIdnfDoervmaetlioopnmeCnentte(rO,ECWDas)h.in1g9t9o3n.,ODE.CC.D Guidelines for |
{ H`Easmtiilmtaotinn,g MM.eAd.i,anR.LCe.thRalusCsoon,ceanntdraRt.iVon.sTihnurTsotxoinc,ity19B7i7.oaTsrsaiym.meEdnviSrpoena.rmSacin.-KTaercbhenrolM.et11h(o7d):1for |
i 714-19; Correction 12 (4): 417 (1978).
i|
UCoSncEePntAr.at1i9o9n3.(ICAp)LiAnpeparroIanctherp(oVleartsiioonn
M2e.0t)h.odEnfvoirroSnumbleentthaall
"Toxicity:
Research
LTahbeorIanthoirbyi,tioDnuluth,
{
Minnesota.
i
SSppoonnssoorr:St3uMdy CIoDmKpRaIn3y3S
5
'
001375
R coms in
TABLE 1. ECq'S, 95% Confidence Intervals, and 4-d NOEC'S from Algal Growth Inhibition `Test with R1335
| mee [w Tw TT ew] EARS crows [mwme [ we w | w ew we || em, ew eee]
[wa| wowvsc |awc usy]
Sas eT eMnee | | eSEsEeS [maeeem,T w we [ w e w eww|
Coreig [| wa |
* Values Based on Nominal Test Concentrations
;-
`Sponsor: 3M Company
%
001376
E15oiCnoayeoinsseronnio
TABLE 2.
Cells per ml and Percentage Difference from Mean Control Value for Selenastrum capricornutum Exposed to R1335
pedir]
|
i {
8
x3
:7:
5s
3=3
TMa
a=a
o
]
Gis
Me::sa
s:3a pEi3S En2Ss
Saaou
3
so33em
|
8
sa;8te |
3
mFo3ows |
140.2
wwe165
|
;:
>
io.
C
a=o
s
x
13160 3
;
ste | soem
<
MaBcn we3: n | wo|1 e | id2: m | ne2is0e%m i
le>1i n | <<<oS1b | a<l<a1 m | a<oSl1So m I
A5
2i
2<1
a<t
<Sro i
yeCcw
2ts | er<o1 w | t<1im | es1om |
+ PracVeo = 3 Dies fom Cont Moss Vali
+0-4 MeAlga alCon unt = 1.X3 10%
Soanre:sl3yM Coormpianys
,
;
001377
.
EAnsveirCoonrmpeonrtaiToin/nAgSc-DDivuilssitohn
AS Sd 100 5010068
TABLE3. VAavleureagfeorSpSeecliefniacsGtrruomwtchapRraitceosrn(uxt)uamnEdxpPoersceednttoagRe1D3i3f5ference from Mean Control
ear Tw L erent|
[on [me [ee[me |e]
oa00 1
"x= lnCeivoeeInrComlllin-s
:
PurethetcValues = % DifferencefromControlMean Valoo 0.0 MeanAlgaCount = 13 X 100
;
SSppoonosror,S3lMyCToOmFpRanIyS
8
!
001378