Document VK288v73yr7o1pdwojoJxyqGg

<Srjes'i>vvwir:^I*i-i^Ci->-'V'*' "'Tri '- ?wfrft ttats * ilicc* * . . . . . * **" wC,h.^i.wc.!'.i. * ' . } T o .fc ^ TTo\dlftg Ko.fctTTo' dlcd feo. fccTiio. clcd ro.fca io.^la-C 0.1 0.2 - 50 o.> o.t 10 * 0 6 0.5 10 . *5 - 6 2 .. v; 0.7 , 10 V 9 - 6 . 1.0 *io' 10 6 6 . . 5 0 5 4. 5* 5 5. 60 60 6 *. 6 5* 66 53 , The acute oral IPgo values vlth their 19/20 confidence Units * * > * t ver* determined for each species by use of the aethod described by Litchfield end Vllcoxon*. The results of the statistical trestsent of the dosi^e-response date are presented- la Table X U . T*BIE TTl . .- - . m usaa ORAL U>M VALDES ?0R 2 ,A ,5-TRICEL0R0FH^r0r!rACi7IC M I S v a t AIHaiSTg g D 0 BATS, KICZ, GGXHSl K G S *30 CHICS U>so (19/20 e m f U e a c a Holts) Slop . gn.Attn. "Function* 0.500 (0.591 - 0.6tv> 0.589 (0.2*5 - 0.619) 0.581 (0.50T - 0.178) 0.510 (0.211 - 0.I5S) & the fold chease la doses re^^iired to produce one unit teod&rd deviation la response along the line# 3U Litchfield, jP.# and ttlheouon, 7.t 1- fhara. mad rat. Thor* * 99, a Z / fi-,T>- =J* *>?'*> ' ' ` dingle a l doses a? 3#*#5"trlchlGrophcAosyac*tie tci6 as c Q ii percent suspension ir. olive oil vere*administered ty stomach tubs to sised sox groups of rats, aiice end guinea pica. Aliquots or t J parent solution in olivo oil verc administered to chicles. shite ?it* end the cuiaaa pics w `selected fros the stock colonies of this Isbaratciy. * Vhitc sice vero obtained froa the Caruorth 7srcs and Ikr* tepshir? Bed chicks vere purchased frea a commercial hatchery. Jlature, pease adult animal wore used in each instaaeo except for the chicks vtlch were approximately three veeks of see* Sable Z amsarixes the ooaacstratioas cad volumes of olivo oil solutions containing 2,t,5-treklrphcaoayacetic acid administered * to each species. 2SJL *e * *B33COLZ ORAL JOCTCSTRATIOT G? 2,4 ,5-tRICELQSOPHZKOSACmC ACID .- Sneeles Percent `Material Volume Qlven by Stomach gahe in olive oil " temixua <cc.\ ikximun (cc.1 Bats Sloe Guinea Bigs Chicks '' 10 *10 <e 5- -` . b.r 0.0 1.0 0.3 - - 2.9 0*3* 2.9 5.8 * Mortalities resulting frea the various single oral dosages ef t#4#5-^rithlorophenoyacetic acid to the experiaental sniaals ere given in Sable ZZ. All surviving nrtiaai sere observed until it vas certain that they had fully recovered (usually about two seeks) * e Spsptosa of poisoning* particularly with tho higher doses, v Qi * axla esd myotonia. ^ ' 8326931 I T The Litchfield end Vilcoseoa testa for parallelism of tvo Q i w i end estimate of relative potency revealod that th* 2#t#5-triehloropbenoxyoeetic cold a* significantly (19/20 probability) sera toxle to the* chicks than to the rets. There were no significant differences In either parallelism or rolstive potency os .result of the following comparisons: rota versus nice, rata versus guinea pigs d e e versus guinea pigs, d e e versus chick* and guinea pigs versus chicks. O 8326933