Document VJzLOj7EZbenNYQ30nJnXZjZw

J aed 2 > 4 Pr -- ~ -) ou J) = TE a fd = Lg J ~~ ; PERFLUOROOCTANESULFONYL FLUORIDE (POS; T-7661.4) TOXICITY STUDY BY INHALATION ADMINISTRATION TO CD RATS FOR 13 WEEKS FOLLOWED BY A 4 WEEK RECOVERY PERIOD Volume 2 MIN 3131023622 PERFLUOROOCTANESULFONYL FLUORIDE (POSF: T-7661.4) TOXICITY STUDY BY INHALATION ADMINISTRATION TO CD RATS FOR 13 WEEKS FOLLOWED BY A 4 WEEK RECOVERY PERIOD Volume 2 Sponsor 3M Center, B3uMilCdoirnpgo2ra0t2e6T-o0x2ic,ology, St Pau, MN 551333220, USA. Research Laboratory Huntingdon Life Sciences Ld., WAloeoolnlbeuyryR,oad, HCaumnbtriindggdeosnh,ire, PEEN2G8L4AHNSD,. Report issued: 28 September 2005 Page 366of740 CONTENTS CONTENTS... ct---- COMPLIANCEWITHGOOD LABORATORY PRACTICE QUALITY ASSURANCE STATEMENT... CONTRIBUTINGSCIENTISTS ..c.cvovssrmmes MIN 313/023622 Page - 2 mmr noe -- 6 INTRODUCTION... i ------ -9 EXPERIMENTALPROCEDURE... n `STTEUSDTSYSUBCSHTEADNUCELEAANNDDFSOTRRMUUCLTAUTRIEON. rprm pm--m---------- 21 SERIALOBSERVATIONS..." NECANR DHIO STOLP OGY.S ....Y ovrrmmmmmosnrsnsn ------ ------ 11s9 PATHOLOGY DATATRBATMENT ...I oococuvsorsssveretmesgggmm--m----e----m--ns 2 3 QUALITY ASSURANCEANDARCHIVING PROCEDURES..........roovovorrrmns 26 DISCUSSION... Ets WARE FIGURES 1. BOAYW- eOUiPTgERhNVtALUsES ..c.covrmsmsmrsmssmmmssnnnnss 31 367: TABLES MIN 313/023622 Page 1. 2. FBoood CdOyS-wgUroeu-MpGm7ieP0aUDngHMvEalhAIueNsVt.OA..L.UsN.E.S. sm rrwrrwrrmroreoroerro -- a) 3. 4. H BiAOCG+-T BGRITOOUU1 PPEMMEEAAMO DNVVAIII0 UEESS.E .r.YY ...cvcerrrrrrsmsormsrsseneseeemreen mrmemnrmsososmmsoseoenrr 42 50 5. 6. UOrrignaanlywseiisg-hGtrsO-UgDrMoEuApNmeVaRnIEVSalues .......... ower " error sons" i 86 re 1 7. 8. MMiaccrprpaatthohooolloosggsyy -c-gcgrrooououpp pddipiStSiiibiUDLcUIOOcn. ww wr -- t--wg5 APPENDICES 21.. DaWeielkylpyopshty-sdiocsaeloebxsaemri--viinnndadiivavitiddutuailafiloiSnndoAniInnngsgss ........e...n..e..s..r. mmsmrsornonnnesig 101| 43.. FBooOdcGoYnW-sEnduI-IVmIiCnGUdpAiIvHVitAdTIuaUilESVSoa.l.un.e.sc.....ressmsosmosmenr en sI omes. 23 5. 6. HBiaoecmha--ieIntnddimivOviididuluaasllv0VatAlIguUreEsSyy...........A ....oommomsmmrmmsmimm sormes rore ners. 138 150 7. 8. UAbsroluiteonr-gaianndlwiveiiydguhastlsi- isndividvauleas.l..V..A..L..L..E. S... m--------" " nee 178 rman 2 91.0. InpdHiVvailduueasl fPOArt1hOeIrOiGnICaallUFIR........I.NAoIrNoGSr.m..r.o.r.rerrsmorrroormrowrrecorr.. _" orn 212 mmm. 50 11. Cellproliferation SIlngs.........rormsorrsmrewrrrer a ene 296 ADMINISTRATION OF POSF BY INHALATITOONRATS ccc. --3 MISCCRAONANNAILNYGSTESL.E.C..T.R.O.N..M.ICROSCR OPE EXAMIm NATION AN-- D X-RAY -- ANALYTICAL PHASE REPORT. mans S-- sis HEYUENTRIENSGEDAORNCHRECSEENATRRECHGLCPENCTORMEPLGILAPNCCOEMPSLTIAATNECMEENSTTSATEMEINTISE.... 735 | VIZ mmm TS --") +368 MINIs SCANNING ELECTRON MICROSCOPE EXAMINATION AND X-RAY MICROANALYSIS Plymouth Electron Microscope Unit (SER, 32 > 2& "2yyo Scanning Electron Microscope Examination and X-Ray Microanalysis. Study Number: MIN313R Be imtange!n vs: == BPreryeerpms PlymouthElectronMicroscope -Un J-- se MIN 313/023622 MatearndiMeathlodss 1. SEM examination of inarybaddertsesamples: Stuhbsaunm)plaensd c(rsipspcraopxiimaeldyi0d3(xp0ro.to5cmol)awteacrheecd)u.Dfroymsafmipxleedstwisesrusemsouumpnldso(nsspppelccidmeinnsu70b%s vithcolic siveadbesive, putercoetedwithgodandexaminedin JEOL S600 SEMat SKY. Fourimages,cachatS00xmagnification,werecollectedto llatypicalepitheliamorphology. 2 SEMI Raymicroanofawilneysasmpiless: DraytaswaemrpelcelsalcstsuepdplaineddawnaelryesecawribtohncaonsOtxefdoarnddInesxtarmuimennetdsiInSI8SJXE-ORLa6y1A0n0sSyEsMasytst1e5mK,V. Xomy 1370: MIN 313/023622 Critical Point Drying (CPD) 1. Fixfreshlyexcisedsampleappropisely (eg.2.5% luarldehydein 01Mphosphatebuffer) 2Washi buffer 3 15mines 3. D3e0ib 1n5em%thir ann-oelssa eries: 5700%% 1155mmiinneess 910%011505mmi%inuns.u.s 100% +15mites FillCPDchambweirth100%anedcobo a010nCoorlles 5 PlacespeciaiCPmD echanmbser 6.Closeandfloodwithliquidcarbondioxide 7 Extalcohol fromchamber. &Leavesample30mitesinchambertoinflatewithcabon ode. 9.Eastremaining ethanol. 10. Hestchaomcbiaeporint 1. Ventcarbondiode gasfomchamber 12Removesamplefrom chambersndmountforSEM examination am: MIN 313/023622 Results 1. SEMExaminaionofUroary BladderEpithelium. Sraemmpailnee1d9sd-oilmyagtewquiamliatgye,suwndeeerthaekoep.eTrahteiengpciotnhdeiltiiuomhnsaudsseedpfaorrthefsrtuodmy,twheesreampalpeprnoopcreills 10 produceadequate images ofthe sample m: NTSC CTI FY wa Soi heAA Felina re a She MIN 313023622 TReT SOR le TSE Sin BAeE RS eETEE Se aE FRpEEpEnn BIS RNEE RE] Se Yordlei Hen EEACL Bee Gyo A Iii iin R MEREa CME nRE he A5 E SRl E Se E TR ni Ea Gi oE tsR re or|{ a re RRe AL SeCORE emSaEnCn eREdE A Ea T B np I am GR SvO oedo GEe ES el TA ee Tee SA Sod Ws sve am NEESER EE mn RlRTs ease ES A Ee ae Cae Ree ANE sto: Te RS Rr at Sh ER MG oe SENET : a = ands 5 he rete Th RNIN Gi : : Fadia stig. TUE pte alte 4 TRERA pu i Lo NS Raa Sh SRA TY DRE Soe Ee CNYAR Ho Os 38 ee, ON BE Ey aR HS ea RE ea = aa Reet (Sine ER S C etA a RSl a CTayen Ra a ta SE TN BeRRS HT EdeeTeE PHD as Cl eR a Bt oa a T SON aai tidER Eeel is Sa Nee al Com ER EER Shey Ea MINE EE TE BEE Sb OtR aE n RR BR aoid EC RaoE SRNE TEEARNT RSOW e a SERSEA Gn fen a BEsic RaiRiSLRETALs Sifee)iLi RTSEeE e:A 0 on E A CAS STE ESEPR r SSIE N BN SA CR EN ITNS1E,ETE N Sein N A YEE &iEihs PaNarGo= s S2ES r RE ie A Fd N SERENaCGiNED Z= n o ea a E E Esn E Sa E N Ro Re SCL Te aHR ERSNELiTKES [pt So SL RR a bt a5 7S HE ( o E Ae rs aEe eSUE i 2 Th E ea iE ia N LS esa a e E Dea la San e S ye a Baa Ch EE ta hr - Gh ti ea ws MIN 313023622 Ra eae RT TONG Oa We L ag e)BE raR SraR BALA se AT Se Tee aNd ai Poa EN eT Fe EySE aeR SOee SI ARtEe fa BUBNEC EN DEAN bret dee Sn eS FN bLaaT tN eT hal ot inemeinei 4Wy 5 SEE SR ENT deena eed FBG SEAa Se ER Mo oe Te AE ACH Sesgan EEG SE Ee REE e aeR B CNS 3 SSis E Ba a Ae BE B3 ERFp ce ies Wier Coober fal i Sie Ma PER Se She ha RI Ae a i rE : nie Flr a] BE en 16 N13 03622 CEH HEE os Die a dR Se Py ! Sane 2s FERRER BE Spas iy a EEN N ET eaRe eAaTl yT Fa A ER eS ea Nid Be ee eyo EeWEE SEE NSEC Ee SUsEA e RRSn ERa SL) End A aT Se to tye ; BSI Ed Foti of GamCe e EG eat ime eye US Zt LG Ard Sy Bo rege vyroCEL Gps a nea CY a EE y BR an L eS S aeTA) =Srer sm ] MIN 313023622 RET a SyEeE Ea rR EE SAE Tape EERREE AER a biERIN ni F Erosg N SEU O ANRE Ee ShER G I e erTrOda ENE EIS ~ Hl Ry 3 R Er RR NE GR as e C SLa r HaS A sBSSOANGiReirgeinEt deNal a BE eh oe ARRELC E ER SESSRd E A i eES ne HemTeSESE EELdS enaeee Se a A S eg R So E eeRiEE a ALO,37 FoR LE or Se SEE sof FRA 5EEgSLeh= otoyy e edhy andn BOALR = LAHtRanaL s Se os Benda Bol dt al ee ee ae ms sis A 7 ONR E T 72 iF a E Mt RaReEO A E Seo R aE Ewaaoa se i aiiye3 SNe OR eal a ToaRE RS gia STR a SQN Es Gaeta NE Eee SE EER Ee PSAs SIREN a gg 2a agra WT in a aySi (re PSSA HO RR or Sleek Se tier) SS a RETS Ta R A CaERE a SigeaSt pe oo S Pee rre e SIC ae E pirE e T aa E Ca R nS es ERD ome ae RN C S SSULEI R RRTR SER alSONRA EE TTL auE, s (RE ee alte rsPARENA a a S SRRiTv a EaOs ESeSa E El ES S E a een C SRTL CnAiNnE IN HeaeeEa E aI a N 9 MN S136 BPR RT BEN 3 =rrE ae Sade L aSS e S SS GHASaR LGEERJe ay ama a e GO Erk ae Oy Sea Eh EA rie ueSaEl Seeae) T C EE e Be S5a Ne 5 iCSreE fae .l vii e RS EEE late A Re a A E EESiR lCRoE naSTS TEPRSe ER NS NY = PeRremSEea SRTMiRne FHARSRSeUNSCUieRE NSN E oetlisSe n eh nAUEh REIR RTeA EE OR ENS a A RaEdPIRFFT IDeEr FE EEa T S eaT aN RTee RCE ra ah ae eS a i SobRans SOE BE Ta aa K PdNBr Hon 8 S NGa SoaTtSa nERg ace Cl a a m0 Misia a aE Fl R STeEhT BRRR R Fo 0 Sie IRC Re Ry \# I FA LL E odLiR BHRA CRIC ER REINNANRR NGLEL Na 41 | ro es > Sf Anas Sa 3 CsEvaEr Sear cl I IL brHeEIGRE I UE a hirer reg a S a Sa e aRe a SSIS AReh Bea SESE asaeee SEGae ect os eSER NEE es wn MIN 313023622 REE NON SAISNEE E Ea N |SAS AR T CE ERAS CNC TNle EWY4TE esced nS Ri RO FREAD Wa Se EEar S E Ee)ee e E ad SC AREyT a RAS, sE isSS NaeEe TF5S TS Ri.CO BLTNehe atoH SE we bod enE t N glAaEssaya CEE SEa sEIadGRASatinEee PS ER Sea FEA Pse I iAe a3 A LaaeE)EEaNey oe eR Ee st SE Sy ar eee w Arve EE 88 ava sis LE &% Ny Chad. 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Study Number: MIN313 ro Hugnno Scions -- PE284HS UPniveyrsitymocfoPoyomnoMcuitssomtopesUs ` iFnmad Drake Circus: ams as MIN 313/023622 MatearndiMeathlodss 1. SEMexaminationofwirybladdertisussamples: `wSStiubbtasnheaclma)plllaeinsddl(cssrpiilpsvrceoarlxapidombieansetilvyder,i0sedp5ax(pt:reo0t.co5cocolmua)dawwceihrteehdgc)ou.ltDdfrrayonsdmaemxpalemedisnwteedirisenemsoJuuEnnOptiLeeSsd6o(0nss0uSpppeElciieadmtienn1s79u60,s% Fourimages,cach 00xmagnifica,wer oleto lstttypicalpiel! morphology. 2SEMOX-Raymicroanalysisof einesamples: dDartyswaemprlcelslacssiupdp1lidedanwaelyrseecaribtohnaconsOtxefdoarnddInesxtarummiennetds1i13SJXE-ORLey6A1n0a0lySstEsMayts 1K. Xr Liqbuoidnssaumplseisrdr3iesdu,pcpalriebdownceorsetceednntidfeuxgaemdi,nae3d0351decsrcorpilbeetdfroormheacsuhpsplaimepdlheywsaasplleasce.don 8 tas9 Results 1. SEMExamofiUrinnaryBaladtderiEpitohelnium. 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ANALPHYASTECOIMPLCETAED LON March 10,2008 PERFORMING LABORATORY. 30E58oRenseRaerscheDaicvhe SPhtoeoCe: So127Pl2A1106e38901 STUDYSPONSOR 3M`CBourdpiornagt2e0T2ox5i.c0ol2ogy Pal,MN551333220 EROIECT ExPyrgoecnoSltdNuymNbuemrb:eMr:I0N.I7S6. Ton Pages 217 518: aNs13zse -- ExonSmotesaNtanytaFi3n07,o 6,PtOeSE T0i0n1d0pThoeuo oofStSeh.ysbty ysesnds CB aorndTyo Cus p pete r +Coopsa <i Sa FAv aomaian )r py ( SZ -- Tomi Sis a-- lyey vats ssi =pa tetladoson -- rtoenr MIN 313/023622 Enns Noss76 `QUALITYASSURANCE STATEMENT EmxyipcenaReptoesre'sofQthueysoAdsioclsdU,nitPveileudrxyogecneSaaynFNcuomsbeeor0n(2P3y.O0S,7E6 hTe5b6y61844)TWeockrRyeStcyovvyPeenrbya AAldlerviiewes nphtaosCesDRwaesefionc3eWe.ckf,xFcololvnetd SScFostpilnigaiiocEGxoyopdnRLeeseesyPSaancctarsdSOupmiin PArlolcesAsn,gwretyPproonsn,(67hde StudyDistor manget ment. ue LAmBirseyn 2hvDmrei 3RDeusovw Create sDfuttoAmmye Fame Report Review bDume omowm iwmam. WTROND Gum mam moss DCuhoasrt Muon unm om WfrL miems miamd oweos 01004 SDuaTRDeeipreeattto Masia nom ovioos OviGEs ooo ovo over Oia=haba QAau s y Audie f=rarch to, of Eons Psseran? 520: MNs13023622 [---- `CERTIFOIFACUTAHETNTIICOITNY Ti oipr, BpStyNaberC576, Smitty, BfSepCtReaosegrmetor. am Poesn pa Bt eye: rs nd conicsof emhianad i -- Ee 5pl BorenResa FityMason Virraiez Eo ch a= bly StyDis, igi Lit ScieLni: A Ti il Soo n1 SonorRepent, zered,2m Ca--pe--rsrToon BoaRect -- Patera? ss MIN313/023622 ExyenSyNo:3076 `STUDYIDENTIFICATION APdmeisnfissotoroacttiaonnssoaClfDoRnyacFlfuoi1d3eW(ePOeSkEs:FTl7l6o6w1e.d)byToax&i-cWkyecSkRetcboyvueIrnyhdPlesrtyiioodn PROTOCOLNUMBER: amis EXYGENSTUDYNUMBER: 02376 TYPE OFSTUDY: Ansiical SAMPLEMATRIX: RatLive, Seranud Umn,e TEST SUBSTANCES: PPeerrfsfcsroroocaomcestamfoensy|afl(ooPrnFOdsSse) (aPaOdSE), Penadecalorsoctnoic Acid (PFOA) SPONSOR: B3uiMlCdoirnpgo2r0a.t2eT6o.x0i2cology Span MN551333220 STUDY DIRECTOR: T`Feoyn.inKegndLnioyfnSciencesLud `AWooonltlaeryRyoad Huntingdon Eva SPONSORREPRESENTATIVE: J3oMhCnoBrupsocrahtoefTfoxicology `SBUulPdainlgM20N.2585.103233220 PERFORMINGLABORATORY: 3Ex0y5geRnesReeasrecahrDcrhive SteCollege,PA 16801 TAONVAELTYATBILCEA:LPHASE `ESxtupdeyriImneinatiaolSnaDraeD:ae: o120n601 E`xApnearlyitmiecnatPahaTseermCinaotimon pDaDlea:tee:i0130/7o1209n0042 ExygnRecah Pugs soc217 is MIN3131023622 Exp StNyo 05476 BROJECT PERSONNEL TToheeSfotllyoDwiisncpteorofnoarehifsrpomroEjxypweinsRTeesreacJ,hKwenenryHassoiciimdwihgvLiafrediSocsieopcheassneLsiodf. ~ esa: TT Name RichardA. Gazi ElyDeter PastConnaly KaenRita Lawrence Ont. RickeyKey Cos Simons Cuts Key JoeGales CosPeg XisomingZh Toe Present Scent TechnLeiasLcEaMlS Semis Semple Custodio `Semple Cusodien TechLneaisGcMaSl Technician seen Sanit Technician EnResearch s Ps6er2i? MIN 313/023622 Exp Sud No:02376 `TOAF CBONLTENETS TDAIrm TE TellBae G`QOUOADLILTYABAOSRSAUTROARNYCEPRSATCATTIECMEECNOTMPrLIAeNCEmSTAeTEMmENTnoen32 = TAOFBCONLTENE TS convening 5600RDEEFSECRRIEPNTCIEOMNAOTFEARNIAALLYTICALvMrErTrHrcOrDrconcrv moomoo 1123. 66.11..21PPFOOSSF2Ex7tdraPcFtiOonAEPXRGIERA0NS0Pr OSIe T r me em er136 62PreperationofStandards nA dForifationSHOR . rrr 14 I 6.5.1 Descriptionof LCMSIMSTstumentandOperatingConditions 15| 6656..21DPesFcOriApatinodnPoRfOGSUQMuSIansntriumteondttaEnxidaOmoppelnraetCinAgcCaoindoo.sc ... v.1161 0 EXPEDESRIGoNrIeeM reuEe SNMTAL ---- 20 E1D00RRETEeNTIOe NOFr DATAmAND-- SAMPL-- ES... -- A-- R--21 BogenResearch isa: PugeTet21? MIN313/023622 ExyenSady No 023076 LI OFTS ABLT ES Bae TableL SummaryofPOSinControlRatLiverSpleen23 TablIeL SummaryofPOSinControlRatSeisSERIE eer23 TableTIL SummaryofPOSinControl RatUteSEBSere23 em" TableIV. Summaryof PFOAi CoolRat LIVESle evn TablVe. SummaryofPFOAinControlRatSeuts Ample... 24 TableVL. Stems ofPFOA i ControlRtUne SS188 err2. TableVIL SummaryofPOSEinRatLiverCORED]SEBS rrr25 TableVIL Summaryof POSFinRtSeu CORELSSPE rc 25 TableTX. SummaryofPOSFinRtUrineCoro)SATIES vr 25 TableX. SummaryofPOS Fortification Recoveriesi RatLIVE rc26 TableXL. SummaryofPOSForfcationRecoveriesinRaSeu... 21 "TableXILSummaryofPROS ForifcatonRecoveries inRa Uso... 28 "TableXI.SummaryofPFOAFortification Recoveis iRatLIVE. 29 "TableXIV.SummaryofPFOAFortificationRecoveries iRat SE. 30 "TableXV.Summary ofPFOA FortificationRecoveriesi RtUn vr 31 "TableXVLSummaryofPOSFRortificaonRecoveriesinRetLIVE vce32. "TableXVILSummaryofPOSFFortificaionRecoveries in RatSer eee33 "TableXVILSumyof POSF FortificationRecoveriesitRatUo. 34 TableXIX. SummoafPrROySResidesisRatLIVrSADIE ere 35 TableXOX.SummaryofPFOAReds inRat LiverSpierA) TableXL.SummaryofPOSFResidesisRatLiverSETDES ver AS TableXO.Sormmry ofPROSResidesinRtSr SOmpls rere50 "TableXO. SummaryofPFOAResiduesinRatSeu SEBS ene 55 TableOV. Seminaryof POSFResidesisRatSent SETI 60 TableXXV.SummaryofPROSResides iRatUs Semen65 TableXOEVLSummaryofPFOAResides iRatUrineSEIpie even 6) TableXOOVIL SummryofPOSEResidues inRtUrgeSEEDS. TS Pugssor217 525: MIN 313/023622 Bop StyNo: 23076 LOIFFS IGUT RES Bs FE ------ on" Figumed.ChromtogamRepresening20.1ngastandardforPROSadPFOA...81 Figur. Chromatogr Representing 25p/n.sendardfor POSE... 82 Figure. "CPhFrOoAm(aEoxgyrgaemRIeDpr0e2s0e1a6i8o4gCaonCtroololA,RSaet:Li1v0e0r8S0a2m8p)l.e..forPROSind. 83 Figur7e. ECaiyropmeantoIg:am02R0e2p8r7e7s,eSntt:ing082CSo0n2trAo)lr RatLir verSampr le forPOSE Figue. PCFiOrAo,m(aigoegmnReIpDr:es2e0n1ti6n8g2CoCnolnorlolAR,aSteS:er0u9m25S0a2mAp)lecfo oPROvS aned 85 Figure. ChromatogramRepresenting aControlRtSerumSamplefo POSE, (E500ID: 0201682Cl,Se 0926028). 86 Figie10. PCFhOroAm,t(oBgxryagemaReIpDr:es2e0n1t6i8ng3CoCnotnttorollA,RSattUro10e03S02p4)efor PROSandcrc81 Figire11, CEhrgomeatIoDgr:0a2m0Re2p8r7e5sCeuni,nSgea:o1n01oL!02RAa)tcUerroisSampleforPOSE, 88 Fig 12.PCRirOoSma1t0odgrPaFmORAe(prEexsyegnteinnIgD:Co(n2t0r1o6lR84tSkLiAve,rSfert:iedwith 10 gf10o8028f).. 89 Fig 13. PChOrSoFm(aBtxoyggremnIReDp:re2s0en2t8i7n7gCponktrAoSlRea:tL0i2v5e0r2fA)ordctSelgwr ith o90 Fig 14,`COhfrPoRmOaSt1ogdraPmFReOeAe(sEexayigoengICDon:tr2o0l1R6a8t2SSeprkumA,oSreikf:ed0w0i2t0h2140)0g./.m.L. 91 Fig 15.CohfroPmOaStEo(gErxaymgReeapIrDe:se0n2t0i1n6g8C2oSnpokAlR,aSteSe0r9u2m60fo2r8ti)fiedwirth epg/rml.e Fig 16. oChfrPoRmOaStaongdrPFReOpAr(esyenptieng ICDo:a0r2l0R1a6t83USripnkeAo,rSief:ed1w0i3t0h2410).g/l. 53 Figur 17. CPiOrSomFa(tEoxgyrgaemnIReDp:r0e2s0en2t8i7n5g CpoknAr,olSReakU10r1i102o4r)i.fied st p/m.wit9h 8 Figs 18.(CEhryomeaoIgDr:a02m0R3e5p5r3e,sSetpiongoRraItDL:i9v1eSreSku:zp0l2e1f0o24P)ROSand PFcOAre95 EonResear Pageveran) 1526: MIN 313/023622 `BSteyNoy:(3e76 Figare19,Ch2r0o3m51a5t,oSgpronsRoerprIeDs:en2t,inSgeR:a1t0L2i5v0e2rSA)amr pleforPOn SF(Bxygens ID: 96 Fig 20.(CEhxryogmeantoIDg:r02R0e3p4r5e0s,eSnptoinnsgoRratIDS:e6raM-SGeRmpOlUeRo4rWPeEOkS4,ndPFOA A vere mmm mmm ==" `Figure21.`0C2h0r3o5m7a7t,oSgproanmsRoerprIeDs:en1tMi-nGgRroautpSe3rDuamySam2,plSeef:o0r9P2O60S2F8().E.x.ygen ID: 98 Figwe22.(CEir3o9m9s0iIo:ga0mR2epr0es3enSt5pionn7gsoR3raIt,U:ri1nSeSea:mpIl0ef0o3r 0PF2OASRan)drPvFeOAove99 Figure23.0C2o0r3o5m7a3i,ogSproanmsRoerpIeDese1n,iSnegR:a1t0U1si1o0e2S)ar mplefor Pe OSF(Esygn enID: 100 LAISPTPOEF NDICES Rae AppendiAxA. SdtymPrsosot50M0IDNOGVIB3GO(RxSygr enSayNo.e 023-076)ande101 AppendixB APsseytcclMfetuhodo:Merthso(dPhofFSe)a,lxPyesfiaisforonotoheceaDneaeesrimlfioncfaaitooen(PonFfOSs)1id. (EPsyegennMiethaoddNoeBcOAacMdfQ(P2iF3OoA0R)oe7iVn1rRia,0oL1i)over rc,Seirur nm ncdiUr ricne: 139 AnPalyeticcalMietohodr:MeothoFolduocorfiAdten(alPayOSnEf)oetRhsaetDUeareicsii,nafSteiruoomnaonnfdyLiv|er (E37820Method No. ERM-02RE3W-ER02.7LA, 153 BoeResearch ps: Fuge 000217 MIN313/023622 ExSayd pNo e023n476 "TTT 10 SUMMARY. EcdeytgceminnRateisoanochfepxetrrfaicsoodraonocdtnssmslayfaoeodarst(PiRvOeSr,) saenrdum,peacntdawdoicnafelsueormopolcasnfcoirtaced (dPeFtOeAn)tiaoccnoordfinpg teoExryglenMoethrodofEoMoOo2d3c(.P0Oi7S)P()naAcpeopnendsdiigxutoBB)feaygodenfMnoertthyhoed ERUL023-071A(Appendix sTehreulmiamintdovfiqunaent,iTuahsieonlfimoirtPoRfOqSuaanndtPaFOAfionr PatOSliFviernwarastl1v0eng/wgaasn2d510yngg/maLidn2r5at ValindLsifoon sauosieersupmearnforumreed.#TthBeoLgOeRnf(oBcoagcehnmaSttuidiweass:0d3e.te0r7m4inaendid n032m3e0t7h5o)d. Hsoawmepvleera,litqhueotLfOorQsfeorre PFaOnAd wainndePaRnOdS1i0ncgascahmmpalteiwewagsdfeotelrimveiro.edDusinuoghao1enmeld. `shaimpclhenicee,asoendlyt0he.L1mOGLfwoarseuascehdmfaotierbny, f0a2cto--r1o.f0m10Lfolerswsi.nTeh,eaLndO0Q.f1orfoPrOlSivFeirn, seerdumwwicshidnectreeramsiendedhuesLinOgG0f.1omrPLOsSmFpli saelriuqmubotyanafdacitosoomfe2.casesonly 005mLwas. PtRheOaStinltvheesraamtpilveersraamnpgeldefsrroamngneod-fdroemecoend-ldeeveelcsed0l8e2v7e0lsa0g5g3.5,T00hawggae.s1P0rFPOOAeSfFa ddeetcciieddilneavnelysof1t1her5t8iv0negrs0/amm0Lp.lePsF.PORAOiSnitnhtehrerttsesreuumsssunppelsrarnanggeeddffrroomm pnooon se amplest .lPevFeOlc sS1i01t2hi ,er0t0unrd gi/nmeLs.amTphlreserwaangsesdofrPoOmSoFnd.edteecttcetdeidneavnyesoftth4e7r10tnsgerum P`wFaOs1A0iPnOthSeFdaetweicnteedsaimaplyeosfrtahgeeradtfwrionmesnaomnp-ldeest.cidlevels 1 16,600ifm.Thee w"iTnheessvaemrpagleepserwceernet9re6c%ove+rie1s1%+,s1t0an2d%ard+de1v4ia%t,iaonndsfo7P%ROSi1n1%r,teplevcet,vsleym., Tnhde asvaemrplaegsepweerrceen9t9e%cov9e%i,s 1s0a5n%da2r1dd4e%v,iaantidon11f0o%rP2FO1A7%i,nrrestpleicvteirv,eslye.ruTmh,eaanvdweinngee pWeercren9t9r%ecove1r8i%e,s 2%stan1da8r%d,devxida9ti9o%nsfo1r8P%O,SrFesipnecrttiveilvyer,serum, ndwinesamples 20 OBJECTIVE ("PTOheSaEb)j,cptiveoftrispuhaseowasrto d(oPeFtOeSro)m,iancned vpeemnsaodfsepccaavfoorcolorcoafnoecimoc ecsinda(lfPtufFrOoiAnde)elin Specimensofalveadseusemplsscoring fProtocol MIN13(Append A). Bop Research Pugs 110207 528 MIN313/023622 BSe dNo n 02376 3I0NTRODUCTION "aTtisHirveorarn:ddstealm tshpeireesul,us onfghehaenaanlaylsyitsifcaolrmteetdheotdecrimitneadio,nMoeftPhFoOdSofaAnndaPlyFsOiAsfoinr t2e8DPetereminantioanofdPeecAccfidao(PFrfOAo)uibnRo(eaPtFLsrSi)va,eo,PSneceofreiucmrscaonocudutUnlcssoiool"faonnnstdseia(ltsPoFtcOehSe) ~ mreetsuhlotdfocrttlheedd,e"tMeentthioodonfoAfnaPlOySsFifsnfoathievDeestedrmsinuatsiosnoeflPse,cluonrogotchtemessnuslyftoincyall mm" Fioride(POS)inRtUrine, Serum,andLiverbyGOMS." `"uTmhebsetrudMyIwaNsIiSn.sTtehdeaonaolDyeticcealmbeexpre6r,i2me0n0t1a,lwshtaerntdihteeswtaudsySerpeecmobresri2g0n,e2dp0r0o2t,ocnodl tesmlyien experimental mination atewasOctober29, 2002. 4.0 TESTSYSTEM "rTehceecivoeodforlorzaeniovoerdanydsieceruomnuJsudnefo12th,e2m0a0t2anbldanAkusgaunstd7m,a2t0i02fofirfoimcaPtoenlsFweeerze Biclogieals,Rogers,Aransas. Niceetiyv-eedifgrhotzeant olnivee,y inicnetyEixgyhgtenafrsoemruHmu,taindgsdeovneLnitfyeiSnceirenactewsionensSaemppilmebsewre10r,e 2002 mdlogged nbyExygenpersonnelandplacedisfrozenserge ``sSoacmiplaetleodwgiitnhathdicshsnaaidnyo.fSciuosrtaogdey cfoorrdmsawtiilolncbaeknebpetfaotuBnedyignenthReesreaawrdcahtaanpdac8kaugee Cdoypy.ofthstoragerecordscnbefoundfnthe rawdatpackagessocitedwiththis 5.0 REFERENCEMATERIAL "TAhrecsnhaaydeatlsoteadnrdeafridPgtFeOdA.TwhaesarneacleytiivceadlasttEanxdyagredPnoROnSwJalys3,ce2i0v0e2dfartoEmxSygiegnmosaManaaylyt1i5c, a2ls0a0n0dfarormd 3POMSEFawvaisrroenceeoivteldTaetcEhxnyogleongy0andJSoenrev6i,ce2s00a2nfdrotmoeSdifgrmoaz-eAnl.dTrhiceh ndstoredambien. "The availbleinformationfor he referencemel edbelow. CoPmFoOnAd PPROOSSE. BopmSieveinsooNe. sspumsy TNCRANe Pui9n9()EspiommieonsDuc 1smoIiEsQ 96896 c0a6nNueoss EnResearch Page 200217 529 d=oerem p ee | TT iH oA reaA e ) ) .p S H=AeLA HNN I IBTA m $Lo-- eE=ERr ERE-- e m E ISnops 6.0 DESCOR FAI NALP YTITCAI LMEOTHN OD MIN313/023622 ESte dyNosn 023976 es9xatmr1palce1tsi0ovneprrsobwcrioteuhsgTh.ytpueApft11eW0ra2t0femorLr..tTcwhaiettohTnseyorpfuemapWnpardtoerprioaaetnessteahmmeppLleievsse,swsteahmeepsvleeosrcuwmeeraedbfroworin- e1 muqtueotsonfdontehervlerssamwpalsestwaerseehormsogfernoimzeeadcwhistahm3ptliesaunedmizmesLfoorfs- t1iitlee.waAsn aodedesdu1pdertmheasnawmiplsedsewceriresdohteo f8ocron-d2it0ioineedsS.PETchoemsaam.pleTshwerGeecseiaumgcedwserned. elie with 2mlofmethanol.Esch samplewasanalyzedbyLOMSIMSelectrospray. 1.2 POSFExtractionProcedure eAxt5ra0c-t1i0on0KpLroacleigdueo.toAf fheesferriucmstnidowninofesapnrdo0p.1igsifsitmeplleisv,ertwheersaemcpaldesfwoerrtehebed 1060C-80Cfor~3-4miouesa henamdbyGCM. 62Preparation of StandardsandFortificationSoutons soAlsttiacoknsofaPnOdSrwsaolsuptiroenpoafrePdoFOnAAwugausstr2ep2r0e02ansJpyci8f,i2d0i0n2ExydpenmsteotchksodnEdxrM-d 0s2o3-l0v7i1.ngTh1e0smogokfsctaacnhdasradsnlduetrion(scowmerecpedrefpoarrepduraiyaecnodnscaslatactioomoef)1i0n0mfethlanboly, F`rmoLmoftiascshosloutcikonand 1a0gifal.hefovrotlifuimceatiponosa1n00dmrLs.lwuiithonewtahasnporle.paredby ing 1 oAGrLipo/nn.stfaonrdiairadsonsbiangnidngrwhaesvprepmarebyo k10i0nmgl1w0imt.hmoefchhae 10pind.mixed AnsdevtaorfiosutsacnadlarbdrsacioonntsainuinigPsFOtAhnfodlPlRowOiSnwgmaasmparsep:aredlatoofthe 0.1pg. JlCoonre yay oo11 0o0o0s of PFomotdOPRAOS Vouss(a) 21 i1o0 10 Di1d0 o FilCo00e0G5ail). iioo o000o1 i10 0000000052 10 oot meAtshtoocdk sEtXaMn-d0a2r3ds-o07s1iAo.nTofhePsOiScEwkasasnpdreeprasroelduotnioJnoweas1,re20p0r2e8ssstpe+ccifoiendeicntEixoyngeofn F1r9o90m tghiasslclbtyondisai1v0i0a0gg-/0m2Lcgaolifbrtateiosntsaandnadred(dcsoorleoctioendwafos ppraepayreidbnryeahkianngd25mlofthe tocksd ringingthvolumeupto50.withmetancl. EpReh Page tot217 : 531 MIN313/023622 BSie odyNo:n 02376 AsetofstandardscontainingPOSEwas preparedbyionof he 1000 p/m mm. . itilC1on10e00.0(00snl) Vol2um5se(al) Diluted1000(al) FilCone2E.d0 g/ml) 10100000 0si 1f0o 10Fl0 # iotpogE1io000 o0r2?5 1100 F1 s"oTrheesdoicknsurdiangsolruio(nan+d2alCf)rwihcetniononkainndcwaeli.bratDioonsctaundmaredsnootfoastnaniwdoerdne Preperation ca befoundi the ra ctsssiaedwih is port. 63Chromatography `QueanntiofincattiimoneooffPPFFOOAAwnisdP~RO6Sawdis-sc5coampliosrhdPbRyOSL.OPMeSaIkMsSwesrlencootdsperyc.iTihden nyofthecomoempl corespondngfothemalereconimc. Q=3:5umine. PeonsfkPswOieSrEewncaostcdastocuespdlisnihtdhbeyocGoCnIntMoS,iTvheersarmpeleclomisampeootndfiPinOgSoF0wtanhse "nyereenmeiofoPOSnF. 64Instrument Sesitity cTohnecsemraaliloenstosft0an0d0a0rduagmo.uonftPiFnjOoActiendddPiOnSg12t4h2e5cphr/ommsifoxpFrOeSpiFc.runhad 4 651Description ofLC/MS/MSInstrumentandOperatingConditions ramen PESCIEXAPL365BiowoMils cAnsayzree Ieee: SCIEXTurbo SpeyLiquidIntrodoctioneice Computer: DelOptiPlexGX110 SHoPftLwCar: PHoEwSicieePxnaaystc(vHeFkri)SocnaiLesrL10d0 HHPP QVuacauPumuDeygasser opmReet Page 1500217 52 MIN313/023622 ExSayyNoe: 02n376 -- EBPPACuoolsuammapOlveren CoPlLuCmCnoTleummp::G3e5nesCi.sCy fonsChrom;ato2g.1r5maxp0hmym),4, " MoIbniljePehVcaostle(i1A)5o:,2nmMAmmoniumAcetate inWaser MoFboiwlReaPsha:se 0B3):nMeUtmhiann.ol | mo e w%A %1B 25000 10 01% 095 1 0 % "#0 00 I10 2 % 10 Tosmotored: oposite AFmRiOvAe mMeogdieve Tomsii4o3n3M3o%n9iored Remon5T6ime(min) BROS nepuive 493% 88 652DescriptionofGC/MS InstrumentsndOperatingConditions Dnstumeat: `CHherwolmeamttodPoelag6987cr93m0lSpaseahsissr/lGeadmcisvoeddeteectolr Column: `ResekRTX60.mxS0250mm2D,L2dp,aof OvenTemperature: FofolcdOmai4n0.C or 4mn,ramp20C.0 100C,hold TIrnjnescttoer TLievmepTereaatep:erta222r20s0:CC CGaCnHeeraGdasP:rssre: H5peelmfo,r025min. ramp80pein.to60pe,hold 05 mrin.erncpoaf--t8hi0epsenailndy.cae0l3ar0.pe,hodat30 for IInnjjeesctiioonnLMiondeer:: S4pmitIsD tightgs InjePcutgeiDeolany: 21min. Bo Resort Paget600217 533: MIN313/023622 ayeSudNo: 23.076 PIngjecetioFnlVoowltouSmpel:itVert: 1ia0lmin. ESleeactnrMoondMeal:iplier Volsge: FSroMm ATUNE +200V. MDownetlloTeidmeo:ns: i7'ms 9quantiadio;n 131,215(qualifierions) I`nReengtaorn.Times: Be3.w5lmeittPeacskardChemStsaofitwoarne - Townmime mies 66.1PFOAund PFOSQuantitationandExampleCalculation T`Fhiefipeenamkiarreoaiweassomefasopreldaonrdctahleisbtraatnidoanrsdscaurdvaerwdawsegeonejroaceide(dsiintnogthUes.LfiOtMweSgIhMieSd. lcionnecaenrteagrieosnswioans)bdeyteArnmailnyesdtf.sroomfttwhaereequustionngssbxelcoown.senratonsofsanders. The Ehqueasttiaonnda1crdaclulrveed(ntheeaarmroeugartesosfiaonnaplayramfeotuennd)(giennne/rante,dbbaysetdhoenApneaalyksataso)futwsairneg apyrografmo.rTsehreuamEqaunadtiionne.2clelstedtheamosstof nal foundinagg or ver4d Equation Analytfound(g/mL) =(Baskara ntcuep) xAF Where:AF = AliquotFactor ope `EAnaamlyotnfo:und(pb,ne folve andnpn.fo seamanwine)= Where:FV =Fioa Volusman( eamlplfeovf ouln.d(mLa )ofsXamFl pVl(ewuel) i)gDhtE) `DF =DisonFactor FEqouratsiaomnpl3ecsafolretiufaieddthweiptehrkcennotwenacmoonun.tsofPFOA.andPFOS priortoextnction, ReEcoqvueriy :(3)= `Cana.oundm(gopuhntaavded.eadna7l.pi)nc gob) X100% Ancxampleof acalculation singancalsample follows (forPOSonly: Bop Research Paget700217 54 MIN 313023622 Bayi SudyNo 023076. Ratlive sampleExypenID 0201684 Sp A(Se: 1008028),forified 10ng peeaekns == ssaam dope = sa " mhtaodndefadcftoorrlevs) F= E.10 . RIE Topawvotliunmceoums =So0mltDetssd) saalmipgloeowfaecitgorht F SoT From equation1: AnyoundGel) = [351001280-73520180 Fromequation: = Sing `Assy foundGpb) =(5x.7n2 g 0m p lx1) Fromequation: = 103, 2 FReovey = (03T3o5m0-0x210000% = om 662POS QuantitationandExampleCalculation O``nwGeeMmiSgih.tlTlhlieenepaoerfahrkeegaarrdeesaspswaiaocnes)obfcytahHsPesadCmshnpedluetschtreasoaannldsaaorfitdwcnaarsrevaeuwnsiadnsgrgfweoavesaictonenjdc(cesneirdnagibnUoonBshoe.f standards.Th conontraionwas detcmipedfomtheequationseiow, stohEfqeutsaaturinosdnap|rrcdoagcacamlv.teTe(hnteehnaeErqmuroaoutginrotenosfi2osanapualrltamfedotuennhde)Ggoeonecgrtafto,efdbbmaysaeldhoeenfHopuPenaCdkheieaap)spaltsoionnrg ivertnd flfo serum nd ine. Eqsiolns Ansefond(up) = Brekenee)DF Where: DF =DilutionPacir spe EonResearch Page 1800207 183s: MIN 313/023622 TT ExSyayaNose023n976 Eq`Auntaliyotenf2o:und(pm, ggfo iverandpga.fosserum an wine)= `anal.founSd(Vg(pmmL))orSStaWnda@rdxvoVluImeLa)dde. d(nL) hers: SSWV==SSaemmpplleeWVeollguhtme corasamlplcetsheufpoerrlicfeianetdtrwecieotvhekdryn.ownamousisofPOSEpeor fo anaes, Bauaton 3 ERqeucaotvieorny3(9)= (anal found(agmpomu)ntaadvd.esda(plpimn)cr opm)X100%. AnReaxatmspelreuomfsaamcapllcelEatxiyognesniInDga0n20a9c8u7SspamkplAe(fSolel:ow1s0:07024),friedat pf. ith POSEwhere. peskarea = sun slroepcee.p I= esww Spupsmandefadcftorclve) == 1os S`uSnemdpulredvvoollumuem(eaaLd)ded(al) ==u0005 `Volumeinjected(ul) = FromAemqudaytieoln o1:mdyal) = telax1 . Fromequation2: = %6lugnl Analy found Gpm=) 09X 16.XL0u1pin00(m0 l) (5ml) Fromequation: = aspgml FRecovery =(4mxm10)0% Som = 9% EpResearch Pugs 1900217 536: MIN 313/023622 ExStuydyNoe: 02n376 Nloitgh:tlTyhdiisfferxeanmtpfloecmaltchuelavtailounewsasshodownne nsthnegrRoAuWndDeAduTmA.ber, and therfore may be 7.0 EXPERIMENTALDESIGN - Ebalacnhks, tewoofmastarmipxlbeasn(kisvfrorotirfsieedrautmk)nfoowrnLconMceSntMraStioanmsl,yosoisfcioelndsissaimepdloeffoorntiefimedattiax mm" mieothwodco0ndctemhreantainoanl0yzded-i2n0dpslmipclatse., EEaacchh sseatmopflseawmapslexstivcer ossiengumh)esfporpGroOpMiSse aconnacleynstirsactioonnssi,soteod oifdosnaempmleuorxifiboad nak,4ktnwoownmcaomnicexobaltainonkssnfodr-if1i0osdam#plkesn.own 8.0 RESULTS "TIhL.ePTRhOeSPfFouOnAdfionutnhde cionnttholcroatntvoelr,astefriuvme,r,asndeumio,assnadmwpilneessraemlplsesdinreTlabsledsfn `iTsabeldeisInVT-aVbLleTshVeIPLIOXS.FPfoeuankdisnwtehreeconnottodleretceIdei,nsaunymo,fatnhdeicnonetsoelmpslaemspalees comeosthepanoalynteredienitonntimges. In`TdaibvliedsuaXl-rXeIcLo.veTrhieessfovrerPaRgOpSienrtterreactolvievrei,essesut,anadnadrudrdienveissaompnlsefsoarrPeFdOcSailendrfatn lrievsepre,cstievrelmy,.ItnnddivwiidnuealsraemcopvleersiewsefroerP96F%OAi+nt1h1e%,r1at02Iv%er,s1r45,,aanndd ri7n%es2u1np1l%es, PaFrOdAeitneaniTvaeb,lseesrXumI,IaLnXdYw.iTnheesaavmeprlaegsewpeerrcoe9n9t%recov9e%ri,es10&s5%an2da1r4d%d,evaiosdti1o1n0s%ox+ 1s7am%p,lreessaperctdiveelty.eIinndiTvaibdluaelsrXecVoIv-eXrViIeIsfL.orTPhOeSvFeirnagthpretraverr,esecamo,vasdeanwrdiansred d0e4v.i9t9i%onsf1o8r%P,SreFspiecnivaelliyv.er,ser,androesampleswere99% 18%,92% 18%, IPnRciOvSiidenatlhreerastusisverrlsiasmtpeldesnrTaanbgleedXfIrXo.msPonF-OGAeitencttheeeavtesve1r0s5a3m5p,l0e0s0nragng. fom o0nPdOeSFdetelcetveeldsins08n2o7f0tnheg.aInvievsisadmapllreess.ultIsarne lisdtedriiesnuTlvtasblireeXsdXe.udThieaTrabwllaes XInXciLvPidRaOlSreisnultthserraetlissetredumsaiTmapblleesXrXaIngLedPfFoOmAinnot-heetrecttseedrluemvselps tlo158r,a0n0ge0ndfrgo. wDaons-1de0iPecOiSedFedveelte012,1m0y0nofgthae r.atInsievriudmosaalmrpelessu.sInrdeilviisdtueadl rneTsaubslaeeXiXItId.Tfohere `TamblLeXXnIdViv.idPoRalOSeisntshaereHtwiidnisnTpaibelesXrXaVng.edPfRroOmAinonnt-dhteacieidoleevsealmspl0es4:710 `rangedfom no detectedlevels 18,600ng/mL. IndreielsvareiiteddisuTablle ExeResarh Page20ct217 557: MIN313/023622 ExSadyyNoe: 02n 3476 XXiVeLdThearTeawbalsenXoXVPIOLS.Fdetectedinanyofthertwin samples.Individualresults 90 CONCLUSIONS - "PTFhOeSr,tPiFvOeA,tsderPuOm,SF0cowoirdnisamoptleesawpperroeprsiuacteesssmfluylilcyaxlcmaectiheodd.and slyaedfor 10.0RETE OFDNATATANI DSAO MPLNES W`Rheseenartchhewfiinlallbeansahliytpipcaeldrtoeptohertspsocnosmoprl.eTteh,asldlcoisgionatlipnacplaedrsdfatcilgietnyersapetceidfibcyrEwxydguean suxcah 2csopiinesstorfuamelnltrJwogda,tbso,waevweerlexaactsciogpheesdocofpytoefmtpheerfaitnaellaoaglsywiiclallbreespoortmnidtael.l ohrgepinearliofdaocifltityespsecpifiiceadwidnaOtE,CiDlPlrbinecrieptlaeisnoefdGiotohdeLBaxboyrgaetonrRyePscrraacchtaircchaiveevsiafeodr ian r19c97b,hyENihVevMspCeoCsHdeE.MIS)!T. Renedsamplesof efeence.subtanes ac Bop Research : 538 Page2100207 MIN 313/023622 genSudyNo: 23476 BogenReach 539 Pg 2200217 MIN 313/023622 ExypaSudy No: 23076 Table]. SummaryofPFOS in ControlRatLiver Samples Sponor RovDe Nsenaber Soweraosgp) LToonnie G oolCComAiA l0o0w8 oNoD sesame" LLoannie oGoCloMmGmieAA 0l2o8w Lams ColCmmiA lA w LLaanddde oOoOlMeCcmommiiAr 11082A w LLaandiiane OoOOMlCCeommiiAA 002A8 Lavine ooeiceiA mon Table IL. SummoafPrFOyS in Control RatSerumSamples SpeDnce Boore Nsoeme LTaakwrmae GoooCCeamihA o0s LLaamwse oooOeCaeCneiAA O02m5eA LLaawmse oGooeCaeCelmAA ooumsms LLaaimne oooolnRGCeselAA 00AA LLaawe ooooewanCecmelsA loommooss Sonrnopsial) o w ow w o Table IIL. SummaryofPFOSin ControlRatUrine Samples: SpoBnDsor Boowe Nsenober Sounrdogsmt) LTaomwe GmoonCemmhA l1o0w0sA LLaaimne GooolGnmGeeellAA lloovveeeA LLaainne oooolGGmmeelAA llooom ww LLaarnioes oooiscCeeelrA loios wx NDDip=lNcoasDetjocetceidon(Ara ssthanthelowestconcentrationofthecalbatonsisndards 00001 pgiaL) BorenReseach npn : 540 MIN313/023622 [rr `TableIV. Summaryof PFOAinControl RatLiverSamples Spon Eoos Nseaote Fonrsoexg) LToonme C CMoGCA elomo mo LLaamninei GoCMoMlCMaCmoimAA oiouezA wwo steno LLaanmonne NoioGeMcGmmemAA 2IGAA wwo LLaannne ooNoeeMcGmmeeAA 0o2m mLnaioe oovvecumCwemAA loloAs ow TableV. SummaryofPFOAinControlRatSerumSamples Spomer oDr sNeosbe Feerdognel) LTaawmoen GGoOmeRCGmemAA wB2m0 woo LLaamma GOoRwoCCAA GO2SAmA wo mLamea moooemGmeaA GoEnD ww LLaawwmnose mGoORRCGowumiAA mli0UaA ww --lsLoo awmmeive GownCo omiA cmwme ma mi NW A TableVI. SummaryofPFOAinControlRatUrineSamples Spnor oDva sNoee rewriiogen) LTaowwnns GGoonwcCaoumA o100mA wo g Llaawwnns GcoowCcamaA lomAa wo Llaawwnn oooEsCDCmmAA llooown 0 _ TDulpbeleeaao s weconm ooee cwA c liwomm n aowr ND00=0N1otpD/etnec)ted (Aresfsth thelowestconcentration of heclan andar BoeRevert PAST san: MIN313/023622 EenStyNo: 023076 `Table VIL. Summaryof POSFinRatLiverControlSamples SpoD Bow Nseeer Sumriosea) TamEo omneTsY owo lmioss oowmn neosa Rwyo et. lamims oommm omom w LLaammmm momemn mmsoess w eimomso mowmn misoon `Table VILLSummaryof POSFinRatSerumControlSamples Spon Bowe sa rose -- R TLomoi wnceoe D ewommeas esewgen alawnens oovmee iooes w naneens omowm wloe annses oomwe mweosn Lanes mw dow -- me emer Table IX. Spo SummarTyoofwPOSF insReat UrineControPloSsatmples D o NTeYe emiwyo m LP eno r cums N ous pLorme omms wa wo lLeaomnie counmes momma w Lon eves owes ND =Not Detected BoaReset Pugs25o217 isa: MIN 313/023622 ExSyaypNose023n76 TableX. SummaryofPFOSFortificationRecoveriesinRatLiver SoDmer BDow Nesmeber Madimeiogy Re=o LTaannne GCooliostsspAAr lloowwss. iowa LLaasinidinee oGomses ljoonwass EF3 == rem a2 oommaaesssskico ouwmsm ssow a LLaannae ocmoiesssssaAr lloowns 00 0= LLaassiidainnee oCdloessisRsEe loowan 11005s U|o oomsessiecoouesm sswm 1m00 LLeesoa ooolesssiESAaTA 0o16AA i0o Iii)s Laesnd cOoleisSaxDs I0o6l0os FP0) 0ss aa oomnsseskse omeom ssooo 5I LLeasnian ooodeemsssaEna o01i6em wroy -100 LLaaoss ocooiissshisE ooms F0Y un 66 oomnmsssksce wooemm ssowo @= LLaannie ocmosisEaA 1ImIARA w0o 5 LLaanndnies ocomlsiussassiiooinos FFPY) oaw 55 oomossoesseico mmooan sswoe =5 STODARDDEVAIAVTIEORN.GE%11 *DuplicateInjection RELATIVESTANDARDDEVATION: 11 EonReears 1543: Pa 2600217 MIN 313/023622 Ege SuNot:00y76 `TableXI. SummaryofPFOS FortificationRecoveriesinRatSerum SpoDn BDB es Ne Asaicmdagen Be=e LTaonw GGonoisnasn wwoamx iowo+ . Tosm LLaawnws aonoieennssiesse wwoamx 0 1n2s6 ee MIGMRROOUWPIDDeNyS: CGmONeOISkECC GomNmiDn | 0smo "= LLaomwa eGossas oonmsuae iwoo aa LLaawmnas oenssesn oomassaa = T7o UURRGRGOURPOIIDDUs3P:2 GGOORNMTSSKACCT WBRRIIAA 200) Id LLaawmnss coooenossekra wwoomm 100 LLaanmnsa aooloesskeese wwoosm 1 AIDAMGCRI1ODDuRyyU1P221OCCOHOUMSOGPSCC mETRB s0om m02 LLaano coooemsesa o0AA 100 0- LLoaown ooooilnesskeese iooe 00 wioor GGIIREGGRRDODOueUyyUP22PI11 GGEIBMGGEASSKHCCT IOOEIARR 50000 =ss LLoemoansa ocmxsisaespseAn itoowmaa iry m0n LLuowonmaat ccelsseanB tioowmaa EF0 1100s SSDEDGIOEUP|WWGeeckkRi4CQOOMUIWUSISSSPACC IlOomEwA 22000m00 5s STANDARDDEAVvAeTImOGNE: 1140 *Duplicatenjction RELATIVESTANDARDDEVIATION: 14 Bop Research 44: Pagero217 MIN313/023622 ESy ty Nose 023076 `TableXII.SummaryofPFOSFortificationRecoveriesinRat Urine Spryr BDDp Nesmwbe tAtemotpen) me.ow TLoownws oGlIs ESAAn 1T0o0mAa io o w" - et LLaaimna oieenssSEEED tjoomaa TH ooveskc oommR so "" 10 L|m ocmmieessksao otoomma isoo 1=0s LLaaimnee ooidisesssaAnD 1l0oo0waAA 0 5 Laume omEsEeSsEkcE joomaa 3 "a Lewuis oGoonsesskEAe lioowva i0o 55s LLeawwisn oomoelssssEAs llooov 0 " La"ma ooneassSsEecE ijoours FFor) " Lema caomiesssao imimo =1 n LLaee onoeEssSsEaA w1in0s i0o n" Le"win ooOneesSsRsEc Iowmsi i3o @n & onesskc mm oAmV,ERAGE a#7 RELATIVESSTTAoNDDmARRDDDDEEVVITAITIOONN:: 1113 *Duplicate jection Exp Resach sas: Pu28g02s17 MIN313/023622 ExSanydyNop: 023n476. `TableXIIL. SummaryofPFOAFortificationRecoveriesinRatLiver Spmr EDp eesa Aassmiogy Re=o - LTaanni GGooirsetsSpeAar s lows 0owFe LLeannian ocmomissssisseinse olowwss FFP)Y 55% EEE Bn ormuse sec oom sw i0n LLaonnia oGmoiisssassSpeRaAr UlAA 1h0y 1 LLeonainn oGooiieeusSiAeDe oiouuns = 1=04 |1 oamneesssiice oowuss ssmm =5 LLaavne G@OoIlGeUmSSEEAA 006Am 1io0 m0 LLeenninn coeoslasD lisoma I= a ooumessssskkcc woeeas ssowm 7a LLaanses GoooiIeUuSsREAA niidmm i0o o104 LLaanndiiu caooiesusies wiimm == 66 oomoniseko moeemm ssoom == LLaaoniis mooelsesusiEnA IoEAA 0w 1m06 LLaannad cooloesses imioon 0 11088 55 oowmssoessskcc ommoas ssoomw "5 SToDADDEAAVTEIIOGNE: 3 *Duplicatenjction RELATIVESTANDARDDEVIATION: 3 BogenResch + 546 Pag orn? MIN 313/023622 ExStuydyNoe02n376 `TableXIV.Summaryof PFOAFortificationRecoveriesinRatSerum Spormysr BBwD Nseee AAsdemiogen Rew TLoawws GcoownaSsakAa 0o2m0 io o" usm em" LLaowwnns omooseeasskiesr ooaommk MCGROW DNS GUNOSAC GNU . 00 0 32Ho DGROLUoPwDsy ComIeSnCs Oo2smee s1i0n Lawn colsnsga wasn 0 ~5 LLeaww ARKIDO coooiiseansskksne GIS soassmea Om@A F3S so 050% ARRLOoPwIsDyR GaEoSKsC Oommean isow Laws oomsa om 0 05 5 LLaowwn aamoliesnasskkess omnssm FS MRPIDy121 CIMSTEC OB 5000 Fmd w MGLRaOwIsDUyP12 aoMiGsSnSsHkCa OoTmB 50ry00 i1o LLaemwe ccooeamsesD loomn 0 010s4 SIFGLRaOUwPsID1ey1 cGIoMGiAnSHsC OoWmE SIFGRDOayU1P21 GOSSECT IONIAR S00 S000 10s a LLaawwnn Govoeiasnesa oomma i1o0 @2 LLaawnos cooowioassuesse olooomm 2 1108 STADARDDEVAIvAETIROGNE: e14s *DuplicateInjection RELATIVESTANDARDDEVIATION: 14 BogenResearch 547: Page3000217 MIN313/023622 oyeSty No:23076 `TableXV. SummaryofPFOAFortificationRecoveriesinRat Urine SpBor BBDp Nseab piapmed) Re-no LTaaninnien oGmIeGSESAAT 110000AA w1o0 w5 u LP awn r ooessEEN 0A F=) o= ---- 1I oommsessioc wmews | swoe a LLaawwnns CcoSiSsAa lm0ms0s i0o 01s LLaanda ocmoiisessssRsE loomoosa F nms 2u% aommsseessekco ownmaa 0 LLaawmn omGnoissSseAsA olovvo wwo i106 LLaanw ocioieesssainse otosv =0 1nss ""4 ooxoamssskece oiomn EwYo 1I6 p Lesnneamir nsa 10m 00 J13 p Lawn coter spese ios. 1130 & oonneesssssecc mwos oinw * SToDARDEAVVIAETRIAOGNE. u1s7 *Duplicate jection RELATIVESTANDARDDEVIATION: 15 BopResearch + sa Puge31c0217 MIN313/023622 `Bagge St No:3y 76 `Table XV1.SummaryofPOSF Fortification RecoveriesinRatLiver SpDr BoTDpm Nesmwbe pAomy Re=o TaImo i GcExIsSsEkRs liomaa S s srs - Lam0i: oomusmciSsEeAc m0oAs 55 ums ehnC oommsoesssskasc mmeeaa |Ss m1s La3mi GomosemsssEuAs oomean 55 La2mi aommaTsIsskEcA iomAa 5s =a B2n ooonwesssseesc oommaa 55 on Lamai: oonuumssskeAs m0aaa 55 st Laani oGnusmsssEiac oioasnA 55 == oammsssssseisc owssu s5 1nss Lani oommoskma iessao ss 55 Lo@mi oomemsssksAc oioAs s5 nws Tnoo ooomsssssseesc iomma 55 wIw) LeBnionnumsseess lomss 55 w= Lani aoummussieA oiosmeA 55 o=u 55 ooomssssssaisc owoan 55 2 StooARDEEVIATGIONE. 18 RELATIVESTANDARDDEVIATION: 15 Ee + 549 Page 200217 MIN 313023622 Br SodyNeo 0n 23076 `TableXVIL SummaryofPOSF FortificationRecoveriesinRatSerum SpoD D Boeeesw ttaemgyen e*s JOGToOwAnDS? G TIREA SKD wB2n0 soomis - em IEGGROLOWaPIwDDsNyR: GC@muIWeMEOsXEaD GGBIDDAA LS55 noo TrLGaomwiayn Gommsskee w00m2A 55 u"t G DROO2DDW EGmmSSRKDC mTEAA 55 on SEGaRnOiUoPnSsDyS2 qSWuOeRsD w10a2aA s5 " SEGRLaOnPsSeDN GcIoWmISmKaC Ioamn 55 x "4S5MMGGRR1|DDoOyy11wW2211 GCOMiIOoSS 0NEAA s5 @ SLIenN3sDnG121 CoHMmIERaAD o10w2sA 55 I5l SCIRGNLGansD3sDysn|12 CQQIOuMNEiROAsRC I1iA0oE0wAAa 555 3H"l GIRGLoamnDesy @GusSisCka iEonmAaa 55 5w% T TG G IDwn122 EGOSECD ITEEAA 55 20 STLGanWeaski QCHGuISaAD i10o6nA 55 mww STLGanWaek CoOsISsC 010m0A 55 *5s SROUO GR||OWP WeUeckkPt CGIUDURSUSASD 1t0o0rAn 55 " STOADDEVaAvTIEOGNE. 518 RELATIVESTANDARDDEVIATION: 20 EpRect $550: P3321? MIN313/023622 ExSayyNop02n376 `TableXVIII.SummaryofPOSF FortificationRecoveriesinRat Urine Spr Boge Sa am - --2 Tov GSSA N0e0w0D sA sseospeoh wRsew - T| ommeesisc ooomoa 55 a1s et LLneno GcumSsSnEsAs 0o1n2aA 55 m LonoC m ooummsseka oinoewsa 55 m5) uuo oooosseessxesc oiwoawsa 55 n2s Leama oouomasssseeAs joossoA 55 I ansoomGmmessska o0s6os 55 n &" oommeesssseisc iooewm 5s 1w00 annn o Gomemessss oiiso nu omeesic io 55 s a5" Le3nn comossessskas mlommmsa 55 n5 LaHn oommmeessseac lmoommsA s5 8= 55 oommeesssikcs mwwm 5s I5S STODARDDEVAIvATEIGONE: 915 RELATIVESTAOMARDDEVIATION: 15 BorenResearch : 551 Page34ar217 MIN 313023622 Bop Sad No:0476 `TableXIX. SummaryofPFOS ResiduesinRatLiverSamples Somer BD m eesw Smroos.g + TQosi oowiaes Fm1% - sm viv 23 ao[inreso ooem = m1@ =-i ooogoe ooum =n-s = wooee oouuns = -` aannen ooas aamm 7 QGoenl oinea uomn "`5 ooomuennn oooeaas oSSaemmm w"o oonmoeon:: oooauu aSsone iuunw ammmooosss ooouuunnn isoooowmm =>5 aoannnoens oooeaas 11i02o000w0 i ewons ooem 0 ow =i oonm ooea Siom wwo oonoe oolamn Ssismao0 minv ooonmeee ooonem Sa20a0mo0o --i aoannneee onoaeass EZ2alom == Qooo oinea Ziw N+DDuNpottsDeecticn(rsshnowest librationsandedof0001 g/l) EpReset Pease ssa MIN 313/023622 EenSayNe 023476. `TaXIXbcolnt'e). SummaryofPFOSResiduesinRatLiverSamples Spomor Boove Mesmeber SounFdoasg) | aa 2 oToaass mloewess oasis TTM=m - 2x oooasiosm m= S566 w=e woeos oosms >6 2x mmooss oismies snsso 27 caooeso cioowmmmmR s0umo mx amooee iccoosuumRm. a0i0o0 E5S maooen iioooswmRa. aao0 =0 mooosns oosmm. sai0a F3l aaoons iccoosmumRm. zsme s=ie mmoovevs ciooosmumRm. 2o160o00 a5 maoose iicoonwea. swoomw = aooore ocsomw 21000000 F=y cGoovevs ccoosmem. 2s55a00m0 ssF ccoovveesn oossmm s2s0o0 sen oaoonn ccooswuasmRR. s2oo0o -5 ccooowr iooossmmR. 207000 sw oooovonr oosossam.R. sssi30mm sor oooo oovsas asso0om a ev girecey 20 N*DDup=NlioctsDeetjeecctteido(nArea ssthenlowestcalibrationssndardof 0001 g/l) BoonResearch Page3600217 553: MIN 313023622 BrenSly Nos023076 `TXaIXb(clonte). SummaryofPFOSResiduesinRatLiverSamiples Spoon ErD Nsemober poemroosg ao Tcooosrt ilosouswm Biem " GCoosss ooseem maiws -- - Gaoosss oomaa i1n6 w" oaoon ooees m> -s caommeess ooee i4 "" Qoes iooawra oSoomm >" @wusoo oiwoawsa 2x5m0 a" Gouss iooewra osmm - omuuss oowans ro=m oQuoss ioowwna 2060 sse oGosset iioowwaa 5s2s6s0 == ooassiss oiowsenaR s75s0w0 =s Gcoosstss ooeesm s0in s"e aGooss soesaes 0a0m =5 Gaoosss oosses osew0 se= oGowsss oomsae 02000mm a7 aonngoeo oossee a12wa a5 oamne oosess 2506000000 5> nnmm ooesse 2a70m a" oomm oosw 1sa0o N*DDu=NpoxeDeeecdti(oAnresks thenlowestcaibrnonsandedof0.0001 gin) BoaReset Bue3Tot217 554: MIN 313023622 EyamSty No 023076 `TXaIXb(colnt?e) SummaryofPFOSResiduesinRatLiverSamples Spon Ewe Nseee Foroestsy ao Toonso owemm iwsw # iv" a@ aonuoss woem " Goose. m= " aanons ooieem 51s eo" oannos owwm wnann @@ oanneoss oowoams 2s2o00m0 a oonoes ooemm womm a aanmsse iooswsa a=o o oannss oowoas o2nm ES oannss ooes 0sm m=w oonoso ooem osonm -n oGnvsee oosmm iot0o00 TM aonneos omesm nferw wws oannsse ooeem iEeo E%d oammeers moesmm somo wn oanmees eoemm r1a00o TMTM aamnesse oosmm 2150a00 w oamoso ooesm 2e5o0 oammsse oose 210000 o ose om au N*DDe=pNotttDeItnefcectteidon(Aresks thnowes calibraonsaofn0.d0r00d1 gf) EraRevert Pugs38217 +555 MIN 313023622 ESte yNosn 023076 `TXaIXb(clonte). SummaryofPOSResiduesinRatLiverSaniples Spon Bomwn Nessete Founrtroasg) u0w Toossee mmiemn Ta5s3 - wea* ==u oomnssu mmooaa ass oa 530 i EuS aonmss mmmoaa Fnrs wies = mmssee mmoons Gmse mo nwwsos wwo mamssea moaaa miamoo "o mausses moaasa 221100mm - aonuss ooaers i1s5o0 ES oQoss mooaan 0 a0 "= oosnee oouw mi7w50 a5 oQsoss mmmes a2o == oQsoss mmoaa mmoo Ed Gammss mmoeaa "74 Ey oQnoss mmmmaa 5n3r =5 aossss amamn 5v26 E*l [ ams roy om mTomm E"S aanos moaowm 15000 so aansoo mmaaww aos aa ozmm iam io so oosmee mmoawe ome ios 1 nwoso N*DDup=lNicoattDe ejeectdion(Arsksthenlowestcalibeainstandardof0.0001 gf) Ben Research Pugs 3900217 556: MIN313/023622 BmSady No: 023976 `TableXX. SummaryofPFOAResiduesinRatLiverSamples Spor ore se ron --D DT aTe omeess toouwsn w esnTese p 3 wires :3 [oruRs ioe oie oon nnss i ` oonue ooiaen i .5 oGuoso owemaan = ane ou omnns a[ nn rN ou nFsS 7 aannoo ooeuss 1050 -' a[nrnr ou 1i50 -5 aannnn ooeus Fi w1o o[ no rN oes mFoe nn [GruRs wan F0P 5 aanncse Gum ooumna oes 2200 0 i we aannn ooees ann ou == E 153 [[ rR re imm i1e o[ me rN ou oon0 "n aomo ooeus n ae oa 0020 ww oomone ooaun oa0m >wo oamsus oo oloau wes a=x FS N*DDop=lNcotuDetjeecctteidon(Arasthnowes alibraonsandedof 0.0001 pg) Exp Reset Pugs d0ot217 1857: MIN 313/023622 Exype Sad No: 23076 `TableXX (cont).SummaryofPFOAResiduesinRatLiverSamples Spoor Bowne fsray yFoy s " an oToesss eaeoonns D227 2 sss onsmat Ezl ocossaes oussoo x aos eo 5sa3s sus w= wooow oose nss == Coooe ooess PByi F7l moues sewss ausa =-TM Comoeer mass uwss ES aannee oossmm asizs ES aooor somsss s5u 3 aaunsees oowsss 5ss s=e aaoees tsomsss a512 2= waoess oosss s7ss = caooners osss u9s P3a oceors ssonm a59s9 E%l aooonn wsens usssi 5 [aoonr eo s0as -5 oaoorm oosso s5a6e Ed Ccoos smoosm oprit Fd oaoos oosess.. "swso Ps cosy sms ao N*DDup=lNicoattDeejteecctteido(nAre lesthanlowestclfratonsaonfd0.a0r00d1 g/l) Exp Resarh Page dt or217 +558 MIN 313/023622 Ba Sudy Nyo: 0a 2376 `TableXX(cont').SummaryofPFOA ResiduesinRatLiverSamples: Sponor Boova Nseose Fourndoaug) aor Tooo olososse aooe - ---- aa caooss ooiiss nass oo oie. [Ta -" a[roie oisea pans " [ [r Ty N usss i" aonosn ooies a116 a a[sao ose suts a" aammso oossee =355 as oaommsz ooisse =a -0 aammias ooses -o FstY Gaomses ooess =o s2e mosmse oosiss s-s a5 oammsiies oossss = ome oan awwm- s=e ccomms oosess 226 s= ccmmss ooiess EsAo sFeyr cammss ooeess ETMY -5 aomm ooeess -= s5r aommaa ooeess 50 5 ommm ooeess Fma a@ oommee ooees s6o N*DDu=pNlioctaDteetjeeccttedio(nArelesthan Jowestcalibraonstandardof 0.001 gin) EeeResearch Page 200217 559: MIN 313/023622 ExypnSady No:02376 `Table XX (cont').SummaryofPFOA ResiduesinRat LiverSamples Spoor Be so Pros --Dry DoTmvelsoin eEny -g aa Gammess ooewss 3as ---- ||a o[meas oes x27s aPy wwnoss oosem zs or waomss ooeess 220s "@ Cmsmm ooeess mEno o@- onsne ooeesm 2177 a [anosr oe 7as on wwoms ooes 2504 o oommen woeess musr n= aCmoss ooees is -2) mosses ooeess x773 ne mmosnses ooees mas1 ix a[mrsr oe 2x16 3 Gommse owoeess 11946 Ten ocmmssw ooeess zuzo TMn o[noers oes aws ne wanosse wweess 16 ES0 wmosse oosess 7a0 ao ome oem ze ND*Dop=lNiocattDeetIenjcetcetdio(nArelesthnlowestcalicaonstandardof 0.001 ign) EponResearch Pages ot217 + 560 MIN 313/023622 EgonSud No 023076 `Table XX(cont').Summary ofPFOA ResiduesinRatLiverSamples Spoor BoDe Nesmwber Sournrdoaxp) Iwr Toossse ieiaah awor messed a= waooss mesa awsa 5 ames joa. as o=- amomss owam was w= oGmmsee emoa ua7s 3 mmssee mmoe 1s0o 3o cmossee omoe 5350 a= mmsee mwee oa0s w r oss y mas 500 wo mones mmome o1x% ESa oomses oomae awns o=e oanoss ieoss sma3r a= omonsss iooieoa wos I5 [ aos rT oon F=l wanoss mions s%s amonsss me 2x5 so"r aonnss emae uass on5 aGoosss iema =92 sr wonoss a me 1926 F0a a[ ne rT me xw2 on oe ma bri N*DDup=lNiocattDeenteecctteido(nArea es the lowestcalibrationstadardof0.0001 g/L) BogenReseach Peston? 561: MIN 313/023622 -------- Bye Sady No: C3976 `Table XX. SummaryofPOSF Residues in Rat Liver Samples Spo Boor Nsoeme pomroseyg) . -1 Tonoess tmoam "wo - >z owoiees mmaa + @ aooneg a wm -` aGooess omammae - oaanoneeo wummae @7 aoonmnaao uwmzm wm ow -: a nmmmwom w >5 aannmn wws w wo oommea ommm w uie oamnaoss wwaoaw 3 > a[rnrs oe iB [ornRr o wxe [anios we isi aonnne a wa i1 [Gooro mua TMn oonmee wmmm uw aaonee wwua i oonnee wmuma x ormme em ow N*DD=iNpotlDeAtolcigedo(Aresesthanlowestcalibration standardof25 gf) BoraReset Pandsoc217 S62: MIN 313/023622 ExypenSayNos 023076 `TableXXI (cont"). SummaryofPOSFResiduesinRatLiverSamples Spoonoe Booe Mesmeber FouPsotsetg) . Fa) Tmoosuss mmAs mwo 2z oComees a mwa so wx wamee mmoens. == [crors wa w 2= o[onoen ws ww TMzn oomoenn ommn a= womoeer ww => oCmoee wwaan w 0= Comee mmwoas w a3 cwmoes mmeasa xw == mwooveess ueaa w == [coore uma w u3 owoore iaaa ow ssx Ccooee mmeaea w so5 Ccooee muimss =-TM wwooew iau a= [wEor we w - Gooosm ewaann w ao wGoovme kpeenn N*DDu=pNlioctaDteeAtlciigeudct(Arealessthnlowestcalibration standardof 25 gal) Boge Rees Pugs d6er217 1563: MIN 313023622 ESe tyNo:n 02376 `TXaXIb(clonte". SummaryofPOSFResiduesinRatLiverSaiples Spon Boown Ssoome Sormomsee) ao Toouusr 02h wo 8 Gooneess kmaias o ------ o Gonoess amaas o o. w" oomoeor mmass >s aomooss wkaann "" [ ase rN ua avo @Goostoo wwaaa w a" wGoonn wkaans w -s oGno ameass wo - oomme emeaass aa oommee amass wo == oammnseiss wisson o E5d o@ousiss ssaaa wk3 se" Goouss ssaaaa w =5 aGoosss ssaaaa "%= Gaomsss ssauaa -= Gonmoooo mmwsam -= ommaen mmsmma w -5 m anmm sea RS o aonn ssaaa N*DDup=lNiocattDeetAelcitqueodt(re esthenlowes clfaion sider of2 gl) EpReva Pagea7ot217 64: MIN 313/023622 ExygenSay No 02376 `TXaXIb(clonte"). SummaryofPOS ResiduesinRatLiverSamples: poser BoDve Msemeber SemPoisas.) a0 Tooeo mmsseess 3 aa aammeess mmsweas w sree "" mommse mmsses. or oomme smowsa " ome mses Pa@ oommey mmsweas a@ aamms smoosa a oomme mmwsam on aammss ssooss ~ ome mss ww 7a aommesn smossn zn ame mses nnn aommees ssooas 7 ames moa o H = aommse mmomaa oor men 32 aamsss oosso 3 ass soa w mTMn aanmess soesn onsen ww comsnseons Ed cms mos ww -= omssee mmeosn N*DDup=NlcosteDAeltieqeudo(tAreaJsthanlowes librationstandof2 g/l) Pgessorany 565: MIN 313/023622 ExypenStdy No: 023976 `TaXXIb(clonte).SummaryofPOSFResiduesinRatLiverSamples --B Sp0or Bp oCDo so tems rose reswoem aa= GGoosss [i ssaa wo o ne 3 u aossusswai Goss oma i"s Qoosss woaaa i ousa jo w wwwo mmsses mmsemm mse wm o ES = aommseo wmemnaa ange msm - Gamnss wwmsa - 5 aamnns ans sa a= o GGoossss ams swns oo 3 F5sS oossss oamaa Gosss maa o 5E5l onossss womn ons sm ow 5"% 7 aamsss a oa ase wma "w r ameee a ase an - Gaooss ase wwaann a lr ome we ow N*DD=iNpotlDeAtleicqtueodt(Are esthanlowestcation standardof25 gf) Bop Rent Peper 566: MIN313/023622 ESuy dy No:e 023076 `TableXXII. SummaryofPFOSResiduesinRatSerumSamples SpDoor oDem Nesmaber Founrtoopsal) DMERGROOUPDDyy cmaoenre tsaamns WD = MMcoROwwiiDDyynr Gmoonnee mwaams et MGGOROWIDNyR 0m6e aoom. w QaUsGGRROoWwDiyDyn oIo woum 6w SeSGuRGROOwwDIyDeyRr Gmooin wcomms a"ra GoirGROWIDn Gm CRommYER amioo TTeMGGRROOWWIIDDNNGRN GGIOT caonmBR a"n0 DBiiGGRROOWWIIDDwN2r GomHy cowmamsR 0sso MMMGGRROOWwwIDDwyeRr GGomss aamme =an0 IIMMGGRROOUUP2PD2uDyeSyz CCaEe uaomsER ms0o IIMNGGRROUOPUSPDIDsS CCaTm mcaoumam 221m00 IMGGROOUwPSIDDyYR GCms aGoms 2200 BIMMGGROOPUPIIDDyyRS CGmD GGaRms 0 240 GRGROOPUPISDDyYR GCam) GGrroookm 2m00 IINMGGROOUWPPIIDDysyzRe mCwL GoaowmsR 2170000 IMRGOOPiPDDyy GCamne ooomm 012700mm GGOOUUPPAiDDy:y nmee Gornoom sswtinm IINMGGROOUUPPADDyyS: Cmaw oOmomsoR msu0i0 IMGGROOUUPPAiDDyySs: mCsass oOmmmmasR onaamo MMeeGERoOwwAiDDGaR Ggooews GoRoUBmER Sssoo N*DDup=lNicoattDeenteecctteidon(Are esth lowestcalibraionstndardof 0.001 pal) Bop Research PageSDot217 1567: MIN 313023622 ExypeNaoSs0u30d76y. `TableXXII (cont).SummaryofPOSResiduesinRatSerumSamples Spoor Boeme Nsesater emrioasgun) TUERGGOORDDyu e mmweosm w - ---- E GORRIDDO: mcouem MGORIDy om Go2esmsa ome. wo w UIERGGROOwRiDDyys: mGwmw oGamms Ry G WECROOPIIDW DYT cIuEm OmAsR sm O TRGROW RIDNT GmwI oOmHaAr TMa5 TWErGGROORPIIDDYyT GGoSw oGwm2a mo0n aImGGROORIRDy GmIwN OowAsmRa w5o SAEEGGROORPIIDDyYTT GmEwS OowEmRR uwseo ASIRRGGROOPPIIDDYyT GGmWs oOwEmSaARR a0so SSREGGROOwRISDDyynS Gmomw oGmEaArR wS10n0 ESREGOORRIIDDyuTI EmWw GlEoAaR aSomm SMRGEROWRIDEyE GoSuSw onmwaa 0z0m SETCGRRORIIDDyuSn GaSmH OmEaSEaAR 2w0o0 SSEEGGROOWIDDyy:T Cbaln oonmsamrR 5w10o0 STEGoORwDyn mGoaw oonsRmR womm TAEEGROROADDyROwMaE oRmsAmaRk 1s5a0m0 SFEEGGRROORRA4DDSy NGaMn oRmAaRr 1151003 CSERRROOPA4DDS:y eGoeSs SOnEsSAIARR 1150260000 EGDO u ows onsman nwo N*DDu=NpokeDTejcetidon(ea essth owesclonsedof 0.0001 gin) BoaResearch Pu stot2r? 1568: MIN 313/023622 ESux ty No:(p 3076 `TableXXII (cont). SummaryofPFOSResiduesinRatSerumSamples Sponsor Bor sa ros R Fra D Nem emieoDpmn u GROUPi IDya1* moss vGaassos wxo mm | acuMGGOOwWiDIyDugmDe m0usMe oGwus. o OWMMGGROOEPUIPDIyDIEyt iss oowssss ow UCGROOPPIIDDyyaEr iosmd oowssms w M RR ODI O yI GmnaMiil0 OoEssR awo GeLGRROOWPIIDDyaiy Colnli OGaEsRR owoo MADGGORWOIWPDIyDEyIL GON2MI3 O0EEsRER 7a450 GMGRROOwwIIDDwIYI GGuolM OwMSERER aan OMMGGOUUPIYDIy Gmialss GG22s0EERR =a0 ASGMRGOOUUPPSIDDi1yyt MGiIsiSs 202sm0mR 1zm6 SM INGG ROUPI IiDyt CoMuSs OGaSomER 110600 MSMGGROUOPWIPDyIInDI CouuMsD GOa2ssmmRR 2m00o0 SSMMLGRROOUWP3SLDDLIyyi oOuMiI oGmEsoEmRR 200m SSMGLROWO3DPyD oOmMNly OGEEERR 11sm SLSGUROGWOPPAIDDDIIyI Galm EGEsEkR mso0m SGOGLRROOWUAPADDIIy GMma2l oEssk a20m0o0 TSUUGGROOUUPPAADDaZyIyI GGMmE OmDSRBR 2m00 USMRGOOPUPIADDIEyyI Gmear OGaEsoRmR 5s1m0o0 OGGRROOUUPDPAyDIiyr Gmiisss sommAn mm0o Gowiby Gnas om i N*DDup2lNicoatteDIentjcetcteido(nAra ssthenowestcalibration standardof 0001 g/l) BogenReseach Pages200217 569: MIN313/023622 EyreSty No 023076 `TableXXII (cont). SummaryofPFOSResiduesinRatSerumSamples SpoDn BoDve Nsoome Sounrdaogser) TE EGG OIDDO u Gomeas lIoioek To er eaGrGrGGOROWOwiwiiIDDDyyyzl oGmoearn oma mwiaa oa 0w5 "rEOROPIDDyyzme Goomees mmima" ww r EOa RIiDDoyzl GGmems mmnEaa so aorrGoROwwDIDyymz Gaemlo moeoam 11000 r CEGRo OIDw yIz mGeN oOmAaR i0ow aGRrGGOowwIiDyymI Gwomm ommoa aoawn. GTREGGOOPwIIDDZEymye Tmneet: ommeak 0EY TTEEGGRROOWwIIDmDyzyt Geks wImaik Esoe TTEEGGORWOWSDDmyye m0e6s mlomka 2s0m0o0 TTEEGGROOWWSIDDmiyyze omeas IuomAmR 2so0m THEGERORWSEDyiDz GGOnL oINaOAkR seawm EIEGRROEWIDDa2yl GCNeM lIOaNOkAR 215m40 TTEEGGROwOWDSyDEIy Gomsa IlomvAak isosaw TTEGEOWGDDyOnIt mGaMl lGoAv@RAR 1o30n00 TTREGGROOwUPDAy2DI1y+ Gamlie OoIvAa o0om TPERGGORODRDyyzIie GGOMNG MOIEAAR 0 on PAREGGRORUOPRDDyyIZZit GGOiW mIaEkA asnm ERa Oa1 _ nies icon pies N*DDip=lNotDejeectdio(n Arashnlowestcbibenionsander of0.001 fn) BomaReet Pes3ocznn +570 MIN313/023622 Bagge Suty Nos23076 `TableXXII (cont'). SummaryofPFOSResiduesinRatSerumSamples Sponsor Bore se ros -- T __ T BD _ new reseoD mn by DOMMGGOPIWHeeitr wdiss llooooommAA ee IGMROOWPIWWeekier GCmMts IloGooAma. SEOLGMROGUPIIOWWaeekPkis Gob0t7s Iiooooa2mmhh MLGRGOURPIWWOeeekPks ooinss lioo2ommh w EGMRGOIIAWOakNPi CCOoNSE Iloo0maAmR 0w0 MGRG OUP WR ekir G0oMSsD ILCoARR s16o0 TEUMGRROPPAWWeakksst GGisiOSL oREaAkR 3150000 EGMGROWaWks GCOoNS lIoEkR 0 zs00 AMGGRROOUPAWWaakks GCOONSS IIRR m200 SMGSIRROGRUOPUWPIaWkese GCHoONMSSH IlEommRn 2)500 SEIGRGRROUPIIWWesesss CCoOnNsS lIoomaonna w TSEEGRCORUPIOWIeUWsePss nGHSD loomaam Rwy STEEGGRROOUPPIWWeeks CGaTne olomnon wo SSEGCROIUPIeWersss GomsetH lloommoon = SSEECRGOURPEIVWeesss GGDoA) Im0m0AaR m=0 SETCRGRROOUWPPiWWeeskss Cmuanl) 0l0RmAARR TRGROUPAWes Gel lomAR awsoo 0 SESGREOUPOies OTEmMaG lw0mRRAR i0600 SFERGGRROOUUPPIIWWeehtr CGONAG Al0mRAR IOFGR|OWUekP Chet Loco 22505000 2x0 IOEG|RWaOkeUr cues loomoan 70 N+DDup=lNicoattDeetnejcctteidon(Arelesthnlowestcalibraonstandardof 0.001 ign) EomReseareh Pagestor21? BE MIN 313023622 BoSagdNoe: 02n3076 `TableXXII. SummaryofPFOA ResiduesinRatSerum Samples SpoDmer BoDe Nsesete Foornoonpen) iGGOo DDy yaeme Gwaams Tarst MGROOPPIIDOnne nCoeme Gomusd 0na ee MHGROOPWIIDDnyeRr mGoene oomu n5e MSGRROOUPPIIDDNNER Gmmm0 oowmne omwr MSGeRROOUPPIIDDYNOR Coasmi Gomss umss SSiiRROOPPIIDDNRy mmmm ooem 1i0 TTReGOOPwIIDnyR GGmm ooomm 500 oDeeomuoPwiioonnme Gmoew Goamuosm u1o0 MMGRooUrP2oDnyS Gmovsss Ooowmns iIom IMRGORPOPIDIyDy Qoosm womae 1m5o0 MMGEROOSIDDyys ammT oasmk 200 IMGGORORRSSDDyyS GQTnS moassk aaw MGGRoOWwSDnyS GGmH ookm 0pel IaGeRGOOWwSIDDyySn QmoSo eooRm 5=0 IGMCRROOSDWyS mGouws osmR 00 IISGROO RDDyyn wGuwSeR Gsausmh oiouw IMMGeGGROOUwPDDyys2ne mOoAmSo GGEuOmR m15m00 IIMGRROOUUPPAADeDysy2 mCSwW OawaRsh 11000 IBMMGRGOOUPPDDyyR GSsS wGaR 11004400 BUGROOWWiDeysn Gcoovews Gomrooumwe 5a0m N*DDu=pNeotsDnteecciteidon(Aras hnowes cabbenionsandedof0.001 yf) EenReser Pugsssat21? 52: MIN 3131023622 ESo tyNasm 023076 `TableXXIII (cont). SummaryofPFOAResiduesinRatSerum Samples Sc BDoow nseee semreoxgen AETROwEPIiGDTyD nOer GGmaoamw 0oonwhse ww ee EWEEGGOOERIIDDysN oGwnum oDwaeen. WSSRGOOOBwIIDDDygyn GGwuumm Gmwaammme WEGGOOi wRIonyDn GGwumowew owommxaa 0o Tm Goooiwwna Gomoow:n ooomwmaea S IeGR owiDOgn Gmuo oommsmmsa R eSEGGoRO wOiwSDO Dgyss mwGouwsms owommemmnss 0 SSoEERwOOwwesIDnDyy mmGmmwsw mwwmoaon 0oo SSEoRwOiwDSeDny GGmoww ommamok SseRGOowwsSDDyyn Gaoww owwomna 0 SSEEowRIIoeOs oGonoa oomnmaa ETGREOGOODRs ygn oGoneel Gem oonxao ox 0=7 as SGEoGwOwDiyyn GGonnae Goanss us SGRGOOWWDDyyn GGnmeax GDaammss py SRGoOwwDioyne Gmoovers rowon moa Gooammmaen ao55s6 NDD u2 opxDleiedn(refs thenowes calfrationsandard of00001 ky) Ese React Pages? ism MIN313/023622 Bo Sad g No:0a 2376 `TableXXIII(cont'). SummaryofPFOAResiduesinRatSerum `Samples: Spoor Row Neswaer SounFdgymt) GTRRGORIDUDOyPU1a ooasses tseman o em" OaMuGGROOWRIIDDyyC oGmoenr sG2muB awss MuGGROORwIiDDyyILl Gowss womm eEROORIPDiDyyLiL2 CCoMMe wm SMGRUOWRDDyiOl Gwoouwl Gsousn aase @iGRowaDw mToRn oa -- mmiiGGROOwwaiDywiml GGoonMz owaonm -2 aemGiRGORWOIwDaWiDIye GGooM owsoms @a ooMMGGOOwIPDINDI IZy Gomsmais oosaes ESSe sS GROM PIDO wRI oosiaiss oomsae sson SSMILGGRROOWPSPDIyDiy2li CoSiIss mossnm 4a DSMMGGROOEwIIDDwyEe oGooann ssam aa SSMMGGOOEPIIDDwyIlE ooniss ssmms nuee SSLMGGRROOWPIIDDWLIEy Guoys 0osuunB E=d SsMMGGRROOWWIPDIWDEyll GGom0es Gsm 3mTM sseetLGEROOwwaaDDwyInll aonnaall os =mn TGMRGOOWwDDywI GGo0n2z Gw2m =100 SSMMGRGOOWwDDiwall GGo0w5 ooosmm El SSMMGGOOwDWmDIwl GGnma oGasuom =se aMGmROoWwwiDbyn Goomeas ognms wmse N"DDup=NlocatsDetjeeccteido(nAreaJsthen lowestcalibrationstandardof0001 psi) PageSTot217 ism MIN313/023622 BayesSodyNo 0376 `TableXXIII cont').SummaryofPFOAResiduesinRatSerum Samples: SForr Bo=e sw o ror E aErGRmow Dy amas e io --m | aorcmoowwiiDwyme oonmea oomm 3 G "rGE omiiDDRgn aGmnaar oomme er@rooGmoRoiwuDiryoiDnyy wGGnmmaass ooolooso o R rmoOwiE namle Gmmoodl loomoan SGroGorwiiDng aGmmoe oomoa GoGrrrGoRowOwwiiiDDnyy GGanmasmmleo omoas TTTEEERGOOwGWiIDOiDyn mGeodt ames looiw loan TTEEGOwGSDOy GGmooss oowm TTEGEOwIGSDDOyi GGmons oIowinmA TeEGGOOwsEDIyDiy GGomenr eslaia fETrERGORODySIe as mes lTlooNlai TTGROOwDyyD, ommuoln ols o TRGREGDwOI cGmoaan lmoaan o TTTEGEEOwGGDyOOe omowmeee oloomaoa RKEGGRRoDwyeinez mamuess llooao SER sty he _ coms ion IS N+DDo=pNloitsDeeiteacceidon(relsth lowestclinstandacdof 0.0001 pin) EmReach Pessoa 515: MIN313/023622 ayeSayNo: 023476 `TableXXIII cont'). SummaryofPFOAResiduesinRatSerum `Samples Spmor BoDwe Nsewober Sosaroxpet) p STMRCGRROOUP [WWeek GGosls 0lo0a2mA w3 ee PMMRGOOWIVWeerkss GGontus loomw ow EDMMGGROOPUIPWWaekdi CQ7u IIoowwaa w OLGGIROOPUIPWWaekis CMiSt iIoowwaa ww UGGOPWWaeekkis CCaomws fIooinea w RMEGGOROPUAPWWaakks GGS0O IIoHEAARR "nao MMRGROOPWWaakks GGoSs lIomRAR 00 EEUMRGOOUPAWWaakkse CGOOMMT IICWETARR mmioo MMGGRR WWakkse GGoov IIoAmA}R mmoo M MRORAWWO keks GGOOMSSHt ICOOWIARR ma00 SISRIGRRGORVOPUIPIWWeeskss GGooMSss lloamnnk wo CSREGGRROOUUPPIIWWeesdt GGoisbs Imomanaa w SSRRGGRROOUUPPIIWWeesdt GGovne omolodnmn w SSEEGGRROOUUPPIIWWeesekkist GGaonbeestt mlomAoan wo SSREGGRROOUUPPIIWWeeeiksr CGbe lmoamomoan as SESGERGORUPOIPWAeWkk GGIm) l0omAmA w STEEGGRROOPUPiWWeekres CGuiusll lloomm2maA w ESERROOUP4WWeakkse COMmG lloomwmmaA FRER O|WWO aakkeer GCMuO lloommmaa LLOERGGRR|1VWOOeertUPkes_GoGonbeett l0o2omn N*DDup=lNicoattDeeteeccteod(nArealessthanlowestcalibrationsander of 0001 g/m) ExypnReseach Pageset217 576: MIN313/023622 BSuo tyNo:n 2376 `Table XXIV.SummaryofPOSF ResiduesinRatSerumSamples Spoor Bow sa Post. --B R GROW Dn Gone N 01a esweDgen " DDARROOPIIDDyySS Gmonee 0miAa ww ee MBGAGOOWIDDyyR CGSe MRO Dye G0 imoAa, lA iGGORORw DIDwyr GQmOTOO OiWAA ww RSeOROPUIPIDDyyS oGEmT l0oAA w SGeiGoRROOPIPDIyDoyN GCmu 0nAA w TMeeGGRROOWPIIDDGyRS wwou lloAla DDeiGGRROOWwIIDDNmRO GCOOW lloollaa MSGMRGOROWWIIDDywT GOOTSS IlOoWkA w IOGNGOROUIPDIDyyS CGSEE niiAn INGGROOUPSDDyse: oGTT eame ww MIuGGRROOWDDyye GGoo mnsamm wo SGMGOOWWDyNer GGooss omeamm B JouGRIODUe P Gm) omens IANGGOO SIDDyyss: GGuom aGmasm w \uGGROOPDeDyyesr: CGamEe oomeenms IIDGAGOORWDDyyeer GGuS wkomm ow GGOOWRDyDe mmoonuM mGasmm MGGRROW DADyyesr GGaos uomemns wo M GRE OUPGS oowsnss ooenme w N*DDup=lNicoattDeeAtleicqtueodt(Are lessthenlowestcalibration standardof25 g/ml) BopResearh rey 5m MIN313/023622 ExSadyNoe02n376 `TableXXIV (cont). SummaryofPOSFResiduesinRatSerum Samples SpoDor Boowe Msember Senrdogsest) B TUERGGRROOUUPTIDDySE eOSTE aomAa w == GZRoGOUwPIeDgrs: moow a oma w UWrRGORUOPUPIIDDYyTS Guos GconaaA ww SIREGGOOWPIIDDYyST GGmoswe GtmA wo GEEOOPPIIDDyyR GGumwml GamAs ww TTRGRROOPIIDSyS wGuwm ommAo HGEGOORPIIDy GGmosw GaEmAa w BBEERGOOPPIIDDYRy Gmoowewd mwmasa w SSREGGROOWPPIIDDyySRH GGmawsnespamns w SSERGGRROOPPIIDDyRy Gmmoss omaAma o SDRrGGRROOWUPPIIDDWySS oGoww iionwaa w SSREGGRROOUUPPIIDDWwSe neSs lloowwee wwo SMEEGGRROOUPIPDIyDsy:S GGouws9 liooowmaa IIRECGRROOUUIPDIYDSY:S GGNoMM liooAmA w sMEGEoOPiWDy G@MmOe mlowAma TTREGGRROOUUPPAADDYySS GMomwn lioowaa w SSREGGRROOUUPPAIDDySw:S GoomMm llooomaa SBRRGGRROOUUPPAADDYySS GGoMM IlooowamaA w ACRERGROOWUPD(oDyr: mmoovess mwmma o N=DDep2NlocsteDeAtleicqtuedo(tArelesthnlowestcalibrationstndardof25 gf) Bop Research Pagetor217 578: MIN 313/023622 `ESxtadyyNop:0e230n76. `TableXXIV (cont). Summaryof POSFResiduesinRat Serum Samples Spon Bop se ,ross B INGRODUwD 2P1 GOOG NI0e02sA Semoiwgeb - MGROVPIDwIZ ABLGIOUPIDI GGONSMT EIAA me MGROUPIDyIN CMGROP1DyIe CO0NS0K8S IHOAAC w i o ORO IDyI2 MGROWP Dy ZI C000M) H10O0AA AAMMLGGRROUOPWIPDIwDIIwzZye OGMOMIIO I10Z2AA GUGLLGGRROOVVPPIIIDZwDI GGMOII IIOGWAA iha MGGRROOWWIPDIyiDIzYe COOMMIIZ IIAA o AMGROWIDNIZ AMGROUPIDYII OQOMMII IIWWXX22AA OOMMGGRROOUPUIPDIwIDIZZw OOMMII I10R2AA SSMMGGRROUOPWIPDIyDI2y1It G0NEMIISS HOODNDAA SSIMMGGRROWOPUSPDyIID12i2mc CCOOOMMIIS 1K0O2KAA wi SMGROWIDI SMGROUPIDNIZIY COOMMIIT OMOKKAA SSMMGGRROUOPUIPDYSIiDZallw GGOMII IIGGAA SSOMLGGRROOUUPPI31DDI2Zwy1s CGOHMMIIS9 II0OOMKGDAA 3 SOLGROUPSDwI2l SMGROUPIIDIY G0MMD OHMOIAA SSMMMMGGRROOUPU4PDAwZDIIzy BC03l1 IIODOKA2A SSIIGGRROUOPUAPDwAIDZZiIw GGNMMZE2 IIODOKA2A w SSMMGGRROOUUPPAADDywIZIlI NGHZMD OIOKNGAA o SSMMOGROOUPUAPDAyIDIIy 003424 IOOKK2AA k D@MAGRGOUPR44DDaOyo1IP2L10_ 0020234S25 tIooAn. 0 N*DDup=lNiocattDeeAtleicqtueodt(Area ssthanlowestcalibrationstandardof 25pg/mL) BogenReseach PageG2ot217 1579 MIN 313/023622 ExStuydyNoi02n376 `TableXXIV (cont). SummaryofPOSFResiduesinRatSerum Samples Spoor Bor sa vost --B D es emioyen GCREGRGORPTIDODTyUIl GQmhas 1I000MAA wwo ------" GaRrGmOoUwPiIDDyyaZe oommaen oaaAn: w SEEGGRROUOPPIIDaDIyy MGMnE OlMoIaAn w GCRRGGRROOUPPIIDI Dyy GGooeMs lloonaem x EEERROOPIDDIyINGG0 lOMAA ww OORRGGRROOUUPPIIDDEiYIT GGUOMSS lOoMkAa EGRRGOOUGPIIDyDEIyZr CGMMRE oloAmA GGRrGGORUOPPIIDYDZIZY GGooaMn llooaan IwS ETERGROOUPDIDIEy GGGMA 0IaMmE TTRRGGRROOUUPPSIDTlD2yyI1t MMsSsS iIo0a0mMaA w TTREGGROOUUPPIIDDyyI GGOoNE lomoaa 3wo TTEERROOBUPISDDGIyU GGOoD looA o HIEERROOUPUSPDIyIDI GGoos ionnAe 3o ETRRGOROUDPyIDa2yi1t CCN0M IITMAA ETGRGORODUPyDI ay CGoa0l HloMonAaA ww TTREGGROOUUPP4DI Dyiyt CGeolM Iioona TTREGGROOUWPIADaD2yy GMa lloa w TMEEGRROOUUPPAADI Dylyn GGOOSW IONMIIAA 3 MEGOUiDIy mow iowa Mo N*DDu=pNoliDeclteaicqtteed(Ares ssthanowestcalibration standardof 25 p/n) ExeRess Pg ot2lT + 580 MIN 313/023622 ExSadyNoe023n476. `TableXXIV(cont). SummaryofPOSFResiduesinRatSerum `Samples Spenmser Bomve Nsemober kdvuosetl) . TGDMRGO IWVakse Guos O0Aa em OOMDGUROGWRIIWOHeeUksPst CGuMdTs I007IT0EAA w SOOLLGGRORPOWPekiIdr CCO0SiS oi0IAMIA MGGROIWWkkss 5 0 OOITAA o IOSLOGROPUPAWWekisr O0 MS IoONAE w3 GMGRROUOPAUVePks eiss lIoormaaAa G EGOOWP eN QODR OoIAA M SMGRG OUPAR WkkSd 0CMMS IioIoEA w SAIMRGGRROOUUPPAIWWeeekktir CCOaOsSsA lIoowia o STRIGEROGURPIIWOWaeUkkePsr bGsSs IA owaza wo TTRGEROGURPIIOWWeUehPdr dGIsNsS IA os w TSEEGGRROOUUPPIIWeerks GCOMSST lOAA w SSEEGGRROOUUPPIIWWeehdr GCHa SlAA SESEGGRROOUPPWAWeekts GCIHAO IIOSDAA ETREROOUWWeakkis CNOMDG IlOMADA o TSEEGGRROOUUPPWWaekkes ICIMMGQ IOIDoAA ETRRGOROUPWIWkekss CGDDQM TlooouanA w FIRGORFOUGPRIWIOWeeUrhkPr GCO0eMSsH IIOSONAEA (OEGROUP Weer_ aise lomoon N*DDup=lNicoattDeeAtleicqtueodt(Aralesthanlowestcalibration siandardof 25 ign) Ee rey 581: MIN 313/023622 Boge Sudy No: 023476 `Table XXV.Summary of PFOSResiduesinRatUrine Samples. ---- Sponor Eo Sosmoer peeros.r i1 Tmsee Imosa Boo, =2 omosses ioovea wo >3 mmomsess oooan" woo "` cmoosses ooas wNoo 5s cComsss mmoeaa Noo o mosmse mmeosa = "7 wmoose ioowwa =1% -5 moomn iwooe 110s4 "> Cooosm smoeaa 0I oIor mwomn moemsa 1 1n wwomn oommaa sas3 w mwooss oommaan wpr 53 mooesss ooess 01% ww omossss omswsa mmo =i wwoonn toommaa -= Fia m[ossoroy ma a5m% kn amomss oowas 1m9o0 wie aCmms omoo 1=0 - cmmoss ooamre 1w06m0 2m maoose ooas 52 Xi DQookkDQetuiveRvee t raionsheap rdvee ocfOl00apr spa)ad foo s an 100. EonResearch Pagesoat? sme MIN 313/023622 ExenSudyNo: 230% `TXaXVb(clonte). SummaryofPFOSResiduesinRatUrineSamples Spoor oore Mesmeber Soundraoisnt) aan Gomne wosa wTM sve azr = aoomn woown ams on. o z=r [mosas oman wr oo oman Nwo 3 == wwuons oomoan x customs NRoY Po EnS comms oommaa TM om ome s1o0 I axn aommse mmeas ame en 1605 ES> omomre oomamn ammo 2153 wr s3e caoommss oommaan = RN monm 50 =5 ccmmss mmwsa = ames amos @= pa br3 ooomeee mmomss aa =3 aaomse ocaonr * amen or 7E2Y0 se owumses oomoanr - cus moa ain0o im F5l oanmee mmoeaan omen oar 0a1i5o0 an aamoeen ooaanr oom oo 1ii5%3o0 NMTDQ NrNotrDQieuesad leRsisaeoawst eeonofnhse nmedtolfaL0c01pplai)dutos on 2100. Ex Research Page60217 583: MIN 313/023622 Eoype Sad No: 023076 `TXaXVb(clonte).SummaryofPFOS ResiduesinRatUrineSainples Sponosor Boowe Nsesaber Fogmrdoogsra - aorr Gaoos loowosn wo * enn? 3 aa aanoeex onmm aves os ww w a" aanneess ooonm ww " awaonu ioonms o a" aommeesr oorwss w7 w" - aonmee oorons ne oe "1oa x7 -a aammee oonneess 5s1s7 - aammean noomse "aas a= aomoe oonmees Pans as2t aameess mmooms ome mom i1s5s sas a5 aaoes waosm Ed mms wos 01 m w5e aaeose ooum = an oem zs 2: s%e waoons cioomumse =ss =5 wooeess oomma nwse --5 oommnees owmearno omen eer 2w0 ss =5 mamearr wmoom @ ame wm 5I = o NkDc aed ve omer vet VQ NokQuite rosetame oes se tinhseirdveedolOi0a0nffalm)ausi 0L0Q. Egg Ress Page c0217 1584: MIN 313/023622 ExpSodyNo: 23076 `TXaXVb(clonte). SummaryofPFOSResiduesinRatUrineSamples SpeDer BoDve Msemaber Sonrdoslat) aE oGnees mmiios. x - -- aa oommeerr mioss wo ames ws. o oamneess mmoss eo ones mos Pa" oamnee mmss 5@s PA@ oommeer miioss anss -" aammeess iioss wais on oammee omess 5126 ES7 oamnee owss ames ais aa1s 1 an oonees: miosn 2m nn oaneess oomm 0= TM= a[meas ms 1m 7 aammeess omoss nms =7 ne aammees moiiss one mos 06 TMn aammeess oioms 11509 n=e aammeess oiiss anew om "a0s m - c[ oseemT oms 76 ae oven mos a HDQ NrtQeuihr(Ripoe hta ceocnhseoiwttx0i20niadbtso 800. BypnReeseh PugseSor217 158s: MIN 313/023622 Baca Sindy No 023076 `TableXXVI.SummaryofPFOA ResiduesinRatUrineSamples Sponor opDe Nsemaec aerop s 1 Taoose loooas. TRo - vemos! =33 c[ ose rN oxen [ro xx 3 - aaooss oovvean ose own ux2o x 5s a[nie om s oo toma =No o7 aommee omoos ES ame mea m1 wm -3 aomm moow m= -5 aamme mmooa E=l w9o un oamo mwoen anes wm w2m4 so u1e a[emrsr oma =so 5 aanmsg oomaar msu oHeo waonegse oomne ne oisd 15 aQmuse ooamer am os wmn TM in1o aamm oorr om os ssoi Pe mu ammwy mo iw ams or 0wo = or aamms moomaa woe oma 2m6o0 0 oramnp e ons oman es HQ NotQuite(pre ba A teof -- ove ati fadbtsaa 8000 EonResearh Pugs69ar217 1586: MIN 313/023622 ExpSady No:023476 `TableXXVI (cont).SummaryofPFOAResiduesinRatUrineSamples Sponor BoDwe Nsomee: proax . Ea)r aGmsooonme "1144 = in? 2z ccmmss oomeasn Ba ams oo wx a= acmmess mooos aw cms oes Nx == aammey oonmees 1p5t4 =3 comms mo =m m7TM acomss oommaa oe == caomss oomma ame wom i= =x Gomoes omorn Fl ans ono 7io 166 a3 acnoewss wa 225400 El2 aaoon oomm 2500 =5 oanmeess oomaar x25500 u0e aaooee ioomar F) ans oma 010 100 *3 aosnss oomma 02 *w "TM [ wowsreor ma onws oma mmom son >= o[menromoyar awno ES oammeonn mamoers otonu Do a" oommee mamoass ssmo NVDQ NNootQDueacst en sporee2owoesotnfosanvtetosf0a0i1spfeam)a iosa LOC BogenRees Page 000217 587: MIN 313/023622 EenSady Nos 023976 `TableXXVI (cont).SummaryofPFOAResiduesinRat UrineSamples Sponsor Eom so rou --D 2o Ce a e FeTiDegen a oma wom } aa " aaoaen iooos aves om x Te" w aaoess mosm" oo ios o C a oamneerr onoemss " ames amo mo w@ oanoem umnoos - mw mm aar "i aa aammean woouwss aa o= aammeenn oownss mez ios pay ss F5Y aaoors oioms m1e s=v aansess oiommm oars a5 aammeass moonso n15s0 == se aammaass ouommm amas oon 2=1 Ea ss5 a amenerooes 1I0 =5 aacmeesn ioonms = aes ons u=t 0 ES= aamneess oiomns Ed anes on m1I ES aomneer iooms one own n1s6 0 a "E Do e ww@ wns-- ow HQ otQuiRespro feo leninstd a LOG Exp Reseach Page or217 E MIN 313/023622 ExyenSud No:023476. `TableXXVI (cont). SummaryofPFOAResiduesinRatUrineSamples Sponnsor Bomva Nemsboer Fonrdopst) aorr Tonoees moiiss TD 4 -- a@ eB oommeer mmoiss amas os. xo a oonneess woass wn ames mos w"o onmeerr wowms a@ oanmeess mioss w a@ aammeess omiss w o n aommeeo mmoss ome ios www 3nw aammeex miooms w n7e aonnees: omomm w nn oaeess ooosn o n=e we aavmeess oosm ames ios = == aomneess muoms =x r7e aommee mmiss n ames ion m1e5 Bs TMn aommees oioms 23 aommeesn mioons TM ome oom ons ns Pywo aoeos i mmmosn o NHDQ.NxxQDueidtee(npfosrhenoawehscaenoofnbesowtobfLi00s1pfnam)a os an000 ExpResarh Page200217 1589: MIN 313/023622 EStp udyNo:a 2376 `Table XXVIL.SummaryofPOSFResiduesinRatUrine Samples Sponnsor orDe Nsewaer Founvdoosptel) "1 Tomsee oooomm wo ~ so 25 oammse oomoa - ams oma o i5 commes oooonm = ame oom ww o. oomsas wooom 7 ame oom w -` aammmo oooom x -5 caomis ooiowma 0 am oom o nwue aammess nnewaa w E2d aammee oonneos 1 ames nea ow Bie m[ oss TT nm i aanngse onnwes ii [TT TT n anes ones o I1 o[nsoEToN nes aos ones I= [[ r= rT a moss iowa z5 oaoos ioowwea = ans oe = oomues oioawa a omy ous N*DDup=lNiocattDeeAtlciiqeudot(Arealessthanlowestcalibration standardof2 g/l) EonReseach PgsT3er217 +590 MIN 313/023622 Bg Sandye No:2n 3076 `Table XXVII (cont). SummoafPrOSy ResidueisnRat Urine Sponsor Bo`Saamples so ros. nx Gne Nemec oes FesndGynt) w - n= amnosnsooowne ow --_---- F3l aanseo ieoawa. ww F0l aannss ooaan w a= omnoees mn w 5u aaonse oomma w H=F amnoss womo 5 aamoesn oomma w oammeer oomo a aces sea o o a= ocmoeen soesae a " Caooss isseons cose sma o wrl ocmoeer sosesa Pa" Caooemn oosses -~ oammees oossoo wo a" aommeeon smseea w - aamoasn oosseo a oammeexr sseeaa ssie oameesn oooon =2 aomeesn ooen o5e aammaess owoonn N+DDo=NpolenDeetAolciigeuder(Aralesstanlowestcalibration standardof 25 pin) ExpnReach Pager or217 501: MIN 313/023622 ope Say No: 23476 `TableXXVII (cont). S`uSammmpalersyofPOSFResiduesinRatUrine Spoor Booge Nsesaber Fundrosyepe seEl 5 Gweores oiwonn amen oma ww w 4 Ea =F se [amoern ia anes oma w w ES5 = ammaess mweeaa [or w w ES 5 aomnaer oosae anal woe w w a@ a oomne owaa ames ow w aa ommeesr woss "a" oammeesr imoanns ames oa wo a aamneess imoewsa wo aonmeesr oaa [rN o aa@ oammeers owmias ames os o o @ oommeenn oosee n ome oe x n oasmee oose ames ea 3n H aammeess eomma ames mm En ome: man N*DDup=tNiocattDseAtleicieeodt(Arelesth lowestclbation standardof2 g/l) BorenResearch Puge7sar217 +592 MIN 313/023622 ExypnSadyNo: 023476 `TableXXVII (cont). SummaryofPOSFResiduesinRatUrine `Samples SpoDor Erne Mesmaber Senvdoosntt) Bh 7=s E onesTRiY os Ww rast n o[ mer oT mes TM anes wa wwo 7TM oanmeess mmeona aneo mwa w 3 oameesn eenn w or oven man w N*DDup=lNicoattDeeAtleicqtueodt(Areasthan lowes calibaion stendadof2 fn) Eo Resear +593 PageT60t217 MIN 313/023622 ExpSudy No: 023476 ExpnResearch + 594 Pa Trot217 MIN 313023622 ExStydNoe02n376 Figure1. TypicalCalibCruravetfoirPoFOnS A FCB raeg mc 1 HAE TAS TEED) TEE mewE eww EpRevs Pgs TBar217 595: MIN 313/023622 BySttyNoos 02m3076 Figur2e. TypiealCalibCurrvaeftoriPoFOnA SA rN gt1 wa ET 2108 7 SEE EET we ow Emit PasT9t217 596: MIN3I3023622 ExSty udyg No:e 023-o 076 Figure3. TypicalCalibCruravetfoirPoOSnF Hele. = co FETE SA iw. = Sr restr se MIN 313023622 ExpStyNe: 025476 Figured. PCRhrOoSmaantodgPrFamORAepresenting a0.1 ng/mLstandardfor IE al EEF J . - TTT OEEtd Co |EEEsEi EE-EF T E= EETE, i Eo Reh +508 Passion MIN 31362 -- Bon Sans Figur5e. ChrReo presm enta ingat25o pg/g mLstr anda ardm for Br EE em m---- enn me megaene - | | | |i| | a. =: `Exygen Research ss | : Page 8201217 M3136 `ESxtady y Nog:0e 23.n 076 Figure6. ChromatogramRepresenting aControlRatLiverSamplefor 'PFOS and PFOA(ExygenID0201684ControlA,Set: esa 1008028) BEZ-: 2 . - - TTT Bro EEE: E=m Fmar:oo : |= - gE == i BE : EEE lE=--F= A z EY " = [-- 0: retin MIN313/023622 [T------ Figur7e. Chro Reprm esena tingt aCono trolg Ratr Livea r Samm ple forPOSF(Exygen ID:0202877,Set: 1025024) BEn r mmr i. : || i | | i | J-- -- aon: MIN 313023622 ---- ExSty udyNpo:e 023-n 076 Figure. ChromatogramRepresenting aControlRatSerumSample forPROSandPFOA, (Exygen ID:0201682ControlA,Set: 0925024) es = . Ce EERE: mli A - - EE S=. EET [= HE, i1 BeypenReseach $602: Page850217 MIN313023622 ------ Figur9e. ChrRo eprem senta ingat Cono trolg RatSr erua mSamm ple we fomrPeOmS,e(oExmygemnIeD:m02n01s682Ctl,Set: 0926028) fr - ERED Fel Ng oe he nm `ExygenResearch. Page 8606217 603: MIN3I30z362 Sy styNozomars Figure 10. Chro Reprm esena tingt aCono trolg Ratr Urina eSamm ple for PFOSand PFOA,(ExygenID: 0201683ControlA, Set: 1003024) -- EET == B= iTHt: : - . . =3- - ETEe=f: == EEE Spt - fee `ESxtadyyNop .: 0e 230a76 Figure 11. Chro Reprm esena tingat Cono trolg RatUr rina eSamm ple forPOSF,(ExygenID: 02C0 trl2 ,Set8 :107 1105 24) FBeRE ShLIITIL EE e amem ee tis + | i |i i | || | : | | Sapna gneo 1605 MIN3137023622 Ba styNyo 05m076 Figure 12. ChroReprmesenatingtContorolRgatLr ive afortimfied (`Ewixtyhg1e0nnIgD/g:0o2f0P1F6O8S4aSnpkdPA,FSOetA:.1008028) EIma --- Bk .: - : :| EE, | i EE == =i lame me Be "OEETEi - T EvpeRes 606 Pessoa? MIN 313023622 `ESxtudyy Nog.0e 23.0a76. Figure13. ChroRepremsentaingCtontroolRagtLivrerfoartifiemdat 1025024) 5pg/gwithPOSF(ExygenID:0202877Spk ASet: ET heAE or RR ge enn y| | i | | ; | i | || | Bors Ress or: Pesos? MIN 313023622 ---- Figure 14. Chro Reprm esenta ingt Conto rolRg atSer ruma fortim fied `with 10ng/mLofPFOSandPFOA(ExygenID:0201682 SpkA,Set: 01002024) S EZR = y :: a EE 3 x : i a She Si == == HI i Bp 608 : estan MNO Bor SyNos sons Figure 15. ChromatogramRepresenting Control Rat Serum fortified `with 5 ug/ml ofPOSF(ExygenID:0201682SpkA,Set: 0926028) me EEE {miemaoncmmsmmmevni.eo ne Comists v - ee EEE aEmlmnTn be SEE ER EEE EEE Ey Rees Pueszac2i? a: MIN3isz36z2 Besos Figure16.ChromatogramRepresentingControlRatUrine fortified `with 10ng/mLofPFOSandPFOA(Exygen ID:0201683 Set: 1003024) ==rs a - : Een : |~ TTT EEE a i | ) Ea B= ke B 3es, | Somes sesso Sow sis `ExygenSuadyNou: 023076. Figure17. ChromatogramRepresentingControlRatUrinefortified at Spg/mLwithPOSF(ExygenID:0202875SpkA,Set: 1011024) BBr,E oSrLrEeEs EER : se - | TM | | | || || ops 161: ssn MIN313/023622 Bop Nord Figure 18. Chro Reprm esena tingt RatLo iveg rSamr plefa orPm FOS `andPFOA(Exygen ID:0203553,Sponsor ID: 91 Set: 1021024) IEE : - ------ EEE: . Ei = | E a E Eke = EE | Boas 1 612 Passer MIN 313023622 Ey soNots02y76 Figure19, ChroReprmesenatingRtatoivergSamrplefaorPmOSF (ExygenID:0203515,SponsorID:52,Set: 1025024) fe - ER Blo am 100 an ents Acoma - Ee 3headspace - - | | | | Boe eh Rss6tar 1 613 MN 31023622 [---- Figure20. Chro Reprm esena tingRt atSo erumg Samr plefa orPm FOS `andPFOA(ExygenID:0203450,SponsorID:86M- Eas GROUP 4Week4,Set: 100202AR) - Ek: EF =F, i il Eiri. E=--r i [SN aie Paesrecn MIN 313/023622 -- `ExygenStudy No. 023-076. Figure21. ChromatogramRepresenting Rat SerumSamplefor POS (ExygenID:0203377,SponsorID: 11M-Group 3Day92, Set: 0926028) me oncom. : ERS Tim an 0 meng regweand mists EE hu [--. seis: Pres ec217 MIN3102362 Ssy ty Nocm omar Figure2. ChroReprmesenatingRtatUorinegSamrplefaor PmFOS `andPFOA(ExygenID:0203573, SponsorID: 11Set: IemmSa0s024%) : : Eras Eee Be ET TR 1 == Su EEE EB - EE = 7 =, i Boas 66 Resa ss `ESxtudyyNog .: 0e 230s76 Figure23. Chro Reprem senta ing RtatUo rineg Samr plefa orPOmSF (Exygen ID:0203573,SponsorID:11,Set: 1011024) :iy ree eee--r--e Sets . EEE San sm vtme | | || | || SI : ee MIN 313023622 Eo syNo:0.76 APPENDIX A Study Protocol MIN/313 (Exygen Study No. 023-076) and Amendments and Deviations Eryn Rees 618 Pugs 010207 re oi carom N32 Bonos yrs " TTY -- esas [E moroco. p Ewma creme. r ~ reso ovuessrcoret oEferrorn =Sime p--o--v = = -- ~ Bott nesta as MIN 313023622 Eo Stoys0276. ------ as . fenti.ngdon ~ conTACTOETALS rm ---- Sos sa Pe aan MN 3131023622 Bop suasmars RG im + LHiufnetSicinegndcoens ~~ - mm - reTsomo emron pion. opnuiasn -- prKr Pr--- EE Eps BE= aErL" boyRentazat HSFoEap tert trGeeeeta ~ -- . Pe iota? ca M3133 J-- e -- --_-- tur, eve Huntingdon *Life Sciences - rcs opmmrao a 50 aare--ne-- atom roveatsr es [ns 7 ~ ro--v--a eh 25 a ote i ----t -- mm rSeE sE cro. e E r Em E. 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