Document VJwGdwv05DQxwjXYx7zjQEvG4
AR 226 _ 0850
ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA)
IESTSU~ BS ~ TANC 000E 00
Identity: A mixture containing perfluorooctanesulfonate, which may also be
referred to as PFOS, FC-95, or as a component of FC-603EF. (1-
Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number is not noted. The test sample is
FC-603EF. Current information indicates it is a mixture of 1.30% PFOS, 10%
diethylene glycol butyl ether, 54.15% water, 22.5% ethylene glycol, 2.6%
propanesultone foamer, 5% Alkylether sulfate, 0.1% N-(3Chiloroallyl)hexaminium chloride, 0.9% Xanthan gum, 0.9% starch, 1.0%
triethanolamine, and 0.05% tolyl triazole.
-
The following summary applies a mixture with incompletely characterized concentrations of impurities. Data may not accurately
reflect toxicity of the fluorochemical component of the test sample.
ME0 TTMH0 OO: 00000000000
Method: OECD Guideline 202
Type: Acute static
GLP: No
Year completed: 1996 Species: Daphnia magna
ana Supplier: In-house cultures, maintained at AScl-DETD, Duluth, MN.
Analytical monitoring: Temperature, pH, conductivity, and dissolved
Exposure period: 48-hours
Test organism age: <24 hours old
Statistical methods: The EC50 values were calculated by the Trimmed
Spearman-Karber method. The NOEC was calculated by Steel's Many-One Rank test (Toxstat software, Version 3.1).
Test conditions:
Dilution water: Dechlorinated City of Duluth, MN, tap water (post
carbon filter) that was aerated for 24 hours prior to use.
Dilution water chemistry:
Hardness:
45 mg/L as CaCO;
Alkalinity: 43 mg/L as CaCO;
pH:
7.92
_
Lighting: Cool-white fluorescent lights. A daily photoperiod of 16 hours light
and 8 hours dark was maintained throughout the testing period. Stock and test solution preparation: A 2000 mg/L primary stock solution
was prepared by diluting 2000 mg test substance in 2 Lof dilution water. The
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remaining exposure concentrations were prepared using a 0.5 serial dilution scheme. Stability of the test chemical solutions: Not noted.
Exposure vessels: 250 mL borosilicate glass beakers containing 200 mL exposure solution. Number of replicates: 4
NNuummbbeerrooffdcaopnhcennitdrsatpieornsr:eplfiicvaetpel:us5a blank control Water chemistry during the study:
pH range (0-48 hours): 7.69 - 8.13 (control exposure) 7.78 - 7.94 (1000 mg/L exposure)
Temperature range (0-48 hours): 20.1-22.4 C
Conductivity range (0-48 hours): 152 pmhos/cm (control exposure) -
Dissol1v4e7d --ox1y5g5epnmrhaonsg/ecm(0(41800h0oumrgs/)L: exposure) 83-86 mg/L (control exposure) 7.4-8.7 mglL (1000 mg/L exposure)
TT
NmgoimLi.nal concentrations: Blank control, 62.5, 125, 250, 500, and 1000
Element values: 48-hour ECs) = 479 (378 -- 607) mg/L.
48-hour NOEC = 250 mg/L
Element values based on nominal concentrations.
SRuebmsatraknsc:e TReesmtianrgkwsafsielcdo. nTduhcetveadlouenstrheepmoirxtteudraepapslydetosctrhiatbemdixitnutrhee aTnedstnot the fluorochemical proportion alone. c0 oncl0 usio0 ns 00000000
T`choenftiedsetnsceubisnttearnvcale o4f8-3h7o8utroE6C0s7; mwga/sL.deTtherem4i8n-ehdotuorbNeo4O7b9semrgv/eLdwiEtfhfeact95% Concentration (NOEC) was determined to be 250 mgL. SPauublm,itMtienrn:esot3aM, C5o5m1p33any, Environmental Laboratory, P.O. Box 33331, St. _ _
pataQuAlTY
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Reliability: Kiimisch ranking = 2. This study meets the criteria for quality
testing. However, sample purity was not properly characterized and the study
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lacks analytical confirmation of the amountof fluorochemical proportion in solution.
REFERENCES
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This study was conducted by AScl Corporation, AScl-Duluth Environmental Testing Division, Duluth, MN at the requestof the 3M Company, Lab Request number P2581, 1996.
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Last changed: 6/26/00
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Location of Raw Data and Final Report |
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S0REFERENCES
AWamteerricaanndPWublaicsHaea.lthAAPRvA.aWnot(tNoPnH,A) 1.1995 Sunda Nero Tor Te Examen
HETasIiaRntE,oSMNL:eA.tCioR.rCti.ho)RnusC1eo,5n3ca4ned1p3tRiVho.noTnnhunTsoonu.ts197m7.eaTrnimm!eHd Sopearmsan eKasot Ath or |
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