Document VJm092O5jmJ44ZnLQ488LN1pw

FOR DU PONT USE ONLY AR226-2897 Copies t E. I, du Font de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine Elkton Road, Newark, Delaware 19711 Experimental Station EYE IRRITATION TEST IN RABBITS Procedure: One-tenth milliliter (71 mg) of solid test material was placed into the right conjunctival sac .of each of 2 male New Zealand White rabbits. After 20 seconds, 1 treated eye was washed with tap water for 1 minute. The treated eye of the other rabbi was not washed* Observations of the cornea, iris and conjunctiva were made with an ophthalmoscope at 1 and A hours, and at 1, 2, 3, 8, and 14 days; Fluor-i-strip stain and a slit-lamp biomicroscope were used at examinations after the day of treatment. -1 Company Sanitized. Doss noi contain T3CA CBf Results: Dose Treatment 0.1 ml Not washed (71 mg solid test material) 0.1 ml Washed (71 mg solid test material) Cornea Ocular Effects Iris Conjunctiva Mild clouding in a general area 1 hr-2 days, decreasing to a local area at 3 days; local area of slight clouding at 8 days. Moderate Iritis, including moderate and slight injection with some swelling 1 hr-8 days. Redness: Moderate 1 hr-1 day; mild 2-3 days. Swelling:Mild 1 hr1 day; slight at 2 days. Discharge: Copious 1-4 hr; moderate 1-2 days; Hemastix positive 1 hr-2 days. General area of slight clouding at 1 hr. increasing to mild clouding 4 hr-1 day; moderate area of slight clouding at 2 days decreasing to a small area 3-8 days. Moderate Injection 4 hrs-3 days. Redness: Mild 1-4 hr, and 2-3 days, moderate at 1 day. Swelling:Slight 1-4 hrs. Discharge: Moderate at 1 hr; copious at 4 hr; mild at 1 day; Hemastli^ positive 1 hr-1 day. Summary: caused general to local areas of mild to right'corneal elr ling, moderate 4Pltls and moderate conjunctivitis with cop. us discharge in both the treated unwashed rabbit eye and the treated washed rabbit eye. Both eyes were normal 14 days following treatment. This material may produce moderate eye Irritation upon contact. If contact occurs, flush immediately with copious amounts of water. -2 SsnlfisecL Doss not contain TSCA CBi Report by: Technician LSF:jrg Date Issued: May 1 Report No. 256-81 Approved by: _ SjJtJ 3 __________ Gerald L. Kennedy Chief, Acute Investigations Section - 3- Com paq Sa,,il,-zo<| .Doesno| onfain TSCA CBf