Document VJgoRxqQd62V0kk9QV7JRyZjq

FOR DU FONT USE ONLY AR226-2779 E. I. du Font de Nemours and Co., Inc. Haskell Laboratory for Toxicology and Industrial Medicine Elk ton Road, P. 0. Box 50, Newark, Delaware 19711 HASF'iLL LABORATORY REPORT NO. 12-82 Material Tested-* Octanoic acid, peiitadecafluoro-, ammonium salt Haskell No. 12,037 C-8 Study Initiatea/Completed 11/1//81-12/3/81 ,, Material Submitted by Haskell Laboratory FOURTEEN-UAY FEEDING STUDY IN MALE AND FEMALE MICE Summary: Crl:CDiB'-l female and male mice were led either (1) C-B, (2)| (1) ammonium omega-hydrohexadecafluorononanoate, or 3U ppm, 300 ppm and 3000 ppm for 14 consecutive days. All female and male mice died after dosing with C-8 and ammonium omega--hydrohexadeca-- 1 iuorononanoate at 3UUU ppm. Deaths also occurred at 300 ppm witl^O--^ a n d ammonium omega--hydrohexadecafIuorononanoate, and at 3000 ppm w i t hjpm^pfind Body weight loss was indicated at 3000 ppm and 300 ppm with all 4 test materials, and at 30 ppm of ammonium oipega--hydrohexadecafIuorononanoate. Liver weight/body weight ratios showed a sharp increase over controls in female and male mice, dosed at 30 ppm with C-8>a>ammonium omega--hydrohexadeca-- fIuorononanoate Dose--response increases were - 1- Company Santeed. Does not contain TSCA CBI seen in C--8 and ammoniun omega-hydrohexadecafluorononanoete.il , ilce showed no dose-related Increases in liver wefght/ltody weight ratios f r o n i o ppm to 300 ppm, however, they did increase at 3000 ppm. Female and male mice fed C-8 and ammonium omega mydro- hexadecafluoroponanoate had heavier livery at lower concentrations than k e e n e x a u j j j j j i | u ] Clinical signs observed for G-S, i^ J ^ T ^ ^ y ^ n i u ^ S S a - h y d r o h ^ a d e c a f l u c r o n o ^ ^ a t ^ ^ j d e ^ w g | h | loss, unkempt head area, weakness and death. ^ clinical signs included: weight loss and deach. In female and male mice, ammonium omega-hydrohexadecafluorononanoate produced greater liver weight changes at lower concentrations than the other materials tested, followed closely by C-8. All mice died at 3 0 0 0 W J C" 8 and ammonium j B m e g a - h y d r o h e x a d e c a f l u o r o n o n a n o a t e ^ O n ^ o u g ^ ^ ^ ^ O O O - ppm withVflHftUnd 2 mice died at 3000 ppm One death* occurred at 300 ppm with C-8 and 5 deahfc occurred a t S O O p p m w i t F ammonium omega-hydrohexadecafluorononanoate. No deaths occurred after dosing at 30 ppm with any of the materials tested. Procedure: Eighty female and 80 male CkL:CtfM mice (age 43 and 44 days, weighing-21 to 38 g) were grouped by body weights into 4 groups of 5/sex/ test material accoraing to computerized randomization. For 4 consecutive days, the mice were housed individua^y and fed eit er ammon urn perf luorooctanoate (- u.rt tiii/v12n,0437/; 14.053) , ammonium omega-hydro-- Q68j hexadecafluorononanoate (H#14,U>5), n* mixed with GPLC (ground Purina Lab Chov^ Each material w a s ? w (controls). 30, 300 and 3000 ppm to female and male mice. The controls e T w i x only, individual body weight. were recorded 3 tioes/weefc. diurnal signs daily and liver weights at the 14-day sacrifice. Results: o Female Mice Concentration PPm 0 30 300 3000 - C-8 H//12.U37 Average Body Weights (g) Average Liver Weights (g) 26 27 18(1-D)** A.D.*** 1.31 3.35 2.98 Average Body Weights (g) 26 26 25 ' 23(1-D) Average Liver Weights (g) 1.44 1.66 2.53 4o20 1 \jLj 2- C o m p ly Sah'bted. cof,(ainTSCA C81 Results: o Female Mice (Coat'd) Concentration ppm Ammonium omegahydrohexadecafluorononanoat (H#14.075) Average Average Body Liver Weights (g) Weights (g) 0 30 300 3000 24 25 20(3-0) A.D. 1.37 4.18 5.11 o Male Mice Concentration PPm C-8 (M12.037) Average Average Body Weights (g) Liver Weights (g) 0 30 300 3u00 32 33 24 A.D. 1.76 4.06 4.16 (H#14.068) Average Average Body Liver Weights (g) Weights (g) 24 1.40 24 2.68 24 2.54 20 3.21 Average Body Weights (g) 35 33 37 27 Average Liver Weights (g) 1.84 2.15 3.63 5.16 Concentration ppm 0 30 300 300U Ammonium omega- hydrohexadecatluorononanoat (Hi/14.075) Average Body Weights (g) Average Liver Weights (,g) 33 30 22(2-D) A.D. 1.77 5.14 4.19 (M l 4,068) Average Average Body Liver Weights (g) Weights (g) 34 33 33 28(2-D) 1.96 3.54 3.70 4.51 o Average Liver Weight/Body Weight Ratios (g/100 g Body Weight) Concentration PPm 0 30 300 300 H//12, 3 T 5.04 12.40 16.56 Female Mice Hirl 4 , 5 7 ------ 'M,7$ Uff14,068 5.54 6.38 10.12 18.26 5.71 16.72 25.56 5.83 11.17 10.58 16.G5 3- - not contain TSCA Company Sanitised-Does J Concentration ppm_____________ c-a Ammononium omega-hydrohexadecafluoro- nonanoate 0 None None None None 30 None None Weight loss, weakness None 300 Weight loss, Weight loss Weight loss, Weight loss unkempt head unkempt head area, weakness area, death death 3000 Weight loss, Weight loss, death, unkempt unkempt head head area area, death Weight loss, death, unkempt head area Weight loss, death >' H/;12,037 Other Names: Hfr`14,033 Purity: Contaminants : CAS Registry: Other Names: Contaminants : iati H#14,075 Other Nanes: o Ammoniun omega-hydrohexadecafluorononanoate ** (//-D) = Number of deaths which occurred at this concentration. *** A.D. = All died prior to sacrifice date. . 0. Louis Dashiell Study Director Approved by: i. y Gerald L . KennedyJr. Section Supervisor Acute Investigations LSF:tac:WP:5.12 Date Issued: March 8, 1982 Report No. 12-82 5Company Sanitized. Docs net contain TSC A CBJ fJL.