Document VJayzVropD4pbraw8zr47yZw8

Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Author: Peter J. Thomford, PhD Study Completion Date: December 13, 2001 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-212 Sponsor Study Identification: 3M T-6316.1 Volume I of XII Page 1 of 4873 Covance 6329-212 3M T-6316.1 COMPLIANCE STATEMENT 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats All aspects of this study were in accordance with the Food and Drug Administration Good Laboratory Practice Regulations as set forth in Title 21 of the Unites States Code of Federal Regulations, Part 58, issued December 22, 1978 (effective June 20, 1979), and with any applicable amendments. 2 Covance 6329-212 3M T-6316.1 QUALITY ASSURANCE STATEMENT This report has been reviewed by the Quality Assurance Unit of Covance Laboratories Inc., in accordance with the Food and Drug Administration (FDA) Good Laboratory Practice Regulations, 21 CFR 58. The following inspections were conducted and findings reported to the study director and study director management. Inspection Dates From To 22 Jan 98 22 Jan 98 02 Mar 98 02 Mar 98 28 Apr 98 28 Apr 98 11 May 98 11 May 98 27 Oct 98 28 Oct 98 28 Dec 98 28 Dec 98 12 Mar 99 12 Mar 99 26 Apr 99 26 Apr 99 02 Jun 99 04 Jun 99 22 Jun 99 02 Jul 99 26 Jul 99 26 Jul 99 20 Jan 00 20 Jan 00 25 Jan 00 25 Jan 00 11 May 00 16 May 00 10 Apr 00 26 May 00 25 May 00 30 May 00 16 May 00 22 May 00 06 Jun 00 07 Jun 00 13 Jun 00 14 Jul 00 20 Apr 01 27 Apr 01 31 Oct 01 31 Oct 01 24 Oct 01 02 Nov 01 06 Nov 01 06 Nov 01 06 Nov 01 06 Nov 01 06 Nov 01 06 Nov 01 13 Nov 01 13 Nov 01 Phase Protocol Review Test Article Administration Analytical Laboratory Inspection Food Consumption Analytical Laboratory Inspection Body Weights Test Article Preparation Animal Observations Protocol Amendment Review Data Review Test Article Administration Protocol Amendment Review Postlife Data Review Data Review Protocol Amendment Review Data Review Data Review Report Review Report Review Protocol Amendment Review Report Review Protocol Amendment Review Protocol Amendment Review Protocol Amendment Review Protocol Amendment Review Date Reported to Study Director and Study Director Management 22 Jan 98 02 Mar 98 28 Apr 98 11 May 98 28 Oct 98 28 Dec 98 12 Mar 99 26 Apr 99 04 Jun 99 02 Jul 99 26 Jul 99 20 Jan 00 25 Jan 00 19 May 00 26 May 00 30 May 00 01 Jun 00 07 Jun 00 14 Jul 00 30 Apr 01 31 Oct 01 02 Nov 01 06 Nov 01 06 Nov 01 06 Nov 01 13 Nov 01 3 Covance 6329-212 3M T-6316.1 STUDY IDENTIFICATION 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Test Material: Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol Sponsor: 3M Toxicology Services Building 220-2E-02, 3M Center St. Paul, Minnesota 55144-1000 Study Monitor: Andrew M. Seacat, PhD 3M 651.575.3161 Alternate Study Monitor: John Butenhoff, PhD, DABT 3M 651.733.1962 Study Location: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Study Director: Peter J. Thomford, PhD Covance Laboratories Inc. PO Box 7545 Madison, Wisconsin 53707-7545 608.241.7207 Study Timetable: Study Initiation Date: Inlife Start Date: Inlife End Date: Study Completion Date: January 21, 1998 January 26, 1998 January 31, 2000 December 13, 2001 4 Covance 6329-212 3M T-6316.1 KEY PERSONNEL 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Study Director: Study Toxicologist: Study Coordinator: Supervisor, Small-Animal Toxicology: Supervisor, Dose Formulation: Supervisor, Chemistry/Tox Support: Associate Director, Laboratory Animal Medicine: Clinical Pathologist: Supervisor, Clinical Pathology: Anatomic Pathologist: Supervisor, Anatomic Pathology: Supervisor, Anatomic Pathology: Senior Staff Scientist: Biostatistician: Principal Investigator, Cell Proliferation: Principal Investigator, Electron Microscopy: Peter J. Thomford, PhD Thomas E. Ryan, BS Cindy L. Powers, BS Jeni Barman, AAS, ALAT, LAT Dixie Bushee, BS, LATG Mark Hoffmann, BS, MBA Donna J. Clemons, DVM Diplomate, ACLAM Robert L. Hall, DVM, PhD Diplomate, ACVP (Clinical Pathology) Ronald Markevitch, BS, MT (ASCP) James L. Carter, DVM, PhD Diplomate, ACVP Kimberly W. Durland, BS, HT Laurie J. Schuller, BA, LAT Cynthia Y.C. Liu, MA Ajit K. Thakur, PhD Carolyn Moyer, DVM Diplomate, ACVP Pathology Associates International James B. Nold, DVM, PhD Diplomate, ACVP Pathology Associates International 5 Covance 6329-212 3M T-6316.1 VOLUME I OF XII CONTENTS Page ABSTRACT.................................................................................................................. 11 PURPOSE..................................................................................................................... 17 REGULATORY COMPLIANCE.................................................................................. 17 TEST MATERIAL, VEHICLE, AND CARRIER ......................................................... 17 Test Material ............................................................................................................. 17 Vehicle...................................................................................................................... 17 Carrier ....................................................................................................................... 18 Reserve (Archive) Samples........................................................................................ 18 Disposition ................................................................................................................ 18 TEST ANIMALS AND HUSBANDRY........................................................................ 18 Animals..................................................................................................................... 18 Identification ............................................................................................................. 18 Justification ............................................................................................................... 19 Husbandry................................................................................................................. 19 Acclimation............................................................................................................... 19 PROCEDURES............................................................................................................. 20 Group Designations and Dose Levels ........................................................................ 20 Dosing Procedures..................................................................................................... 21 Dose Analysis............................................................................................................ 23 Observation of Animals............................................................................................. 23 Clinical Pathology ..................................................................................................... 24 Serum Analyses......................................................................................................... 25 Anatomic Pathology .................................................................................................. 26 Proliferation Cell Nuclear Antigen Evaluation........................................................... 27 Palmitoyl-CoA Oxidase Tissue Collection and Analyses ........................................... 28 Statistical Evaluation ................................................................................................. 30 Survival and Tumor Analysis .................................................................................... 30 Record Retention....................................................................................................... 32 RESULTS AND DISCUSSION .................................................................................... 33 Dose Analysis............................................................................................................ 33 Observation of Animals............................................................................................. 34 Clinical Pathology ..................................................................................................... 35 Anatomic Pathology .................................................................................................. 36 Proliferation Cell Nuclear Antigen Results ................................................................ 37 Electron Microscopy Results ..................................................................................... 38 CONCLUSION............................................................................................................. 38 6 Covance 6329-212 3M T-6316.1 VOLUME I OF XII CONTENTS (Continued) Page SIGNATURES.............................................................................................................. 39 REFERENCES.............................................................................................................. 40 PATHOLOGY REPORT............................................................................................... 41 PATHOLOGY PEER REVIEW REPORT .................................................................... 56 STATISTICAL REPORT OF SURVIVAL, NONNEOPLASTIC AND NEOPLASTIC LESIONS .......................................................................................... 61 COMMENTS ON THE DATA ..................................................................................... 84 CODES, ABBREVIATIONS, AND UNITS.................................................................. 86 General Codes and Abbreviations.............................................................................. 87 Codes for Clinical Pathology..................................................................................... 88 Abbreviations and Units for Clinical Hematology...................................................... 91 Abbreviations and Units for Clinical Chemistry......................................................... 92 Abbreviations and Units for Clinical Urinalysis......................................................... 93 Abbreviations and Units for Urine Chemistry............................................................ 94 Codes for Anatomic Pathology.................................................................................. 95 FIGURES 1 Adjusted Survival Data (%) - Males..................................................................... 97 2 Adjusted Survival Data (%) - Females ................................................................. 98 3 Mean Body Weight Data (g) - Males ................................................................... 99 4 Mean Body Weight Data (g) - Females .............................................................. 100 5 Mean Food Consumption Data (g) - Males ........................................................ 101 6 Mean Food Consumption Data (g) - Females ..................................................... 102 TABLES 1 Results of Homogeneity Analyses (ppm) ........................................................... 103 2 Results of Stability Analyses (ppm) ................................................................... 111 3 Results of Dose Preparation Analyses (ppm)...................................................... 115 4 Survival Data..................................................................................................... 124 5 Summary of Clinical Observations..................................................................... 142 6 Summary of Mass Observations......................................................................... 160 7 Summary of Body Weight Data (g).................................................................... 186 8 Summary of Body Weight Change Data (g)....................................................... 198 9 Summary of Food Consumption Data (g)........................................................... 200 10 Summary of Test Material Consumption Data (mg/kg/day)................................ 212 11 Summary of Clinical Hematology Data - Week 8............................................... 224 12 Summary of Clinical Hematology Data - Week 14............................................. 228 7 Covance 6329-212 3M T-6316.1 VOLUME I OF XII CONTENTS (Continued) Page TABLES 13 Summary of Clinical Hematology Data - Week 27............................................. 232 14 Summary of Clinical Hematology Data - Week 53............................................. 236 15 Summary of Clinical Chemistry Data - Week 4 ................................................. 240 16 Summary of Clinical Chemistry Data - Week 8 ................................................. 242 17 Summary of Clinical Chemistry Data - Week 14................................................ 248 18 Summary of Clinical Chemistry Data - Week 27................................................ 252 19 Summary of Clinical Chemistry Data - Week 53................................................ 256 20 Summary of Clinical Chemistry Data - Week 105.............................................. 260 21 Summary of Clinical Urinalysis Data - Week 8.................................................. 262 22 Summary of Clinical Urinalysis Data - Week 14................................................ 264 23 Summary of Clinical Urinalysis Data - Week 27................................................ 266 24 Summary of Clinical Urinalysis Data - Week 53................................................ 268 25 Summary of Urine Chemistry Data - Week 8 ..................................................... 270 26 Summary of Urine Chemistry Data - Week 14 ................................................... 272 27 Summary of Urine Chemistry Data - Week 27 ................................................... 274 28 Summary of Urine Chemistry Data - Week 53 ................................................... 276 29 Summary of Organ Weight Data - Week 4 Sacrifice .......................................... 278 30 Summary of Organ Weight Data - Week 8 Sacrifice .......................................... 280 31 Summary of Organ Weight Data - Week 14 Sacrifice ........................................ 295 32 Summary of Organ Weight Data - Week 53 Sacrifice ........................................ 325 33 Incidence of Macroscopic Observations - Week 4 Sacrifice ............................... 340 34 Incidence of Macroscopic Observations - Week 8 Sacrifice ............................... 341 35 Incidence of Macroscopic Observations - Week 14 Sacrifice ............................. 353 36 Incidence of Macroscopic Observations - Week 53 Sacrifice ............................. 365 37 Incidence of Macroscopic Observations - Terminal and Recovery Sacrifices ..... 378 38 Incidence of Macroscopic Observations - Unscheduled Deaths .......................... 397 39 Incidence of Macroscopic Observations - All Animals....................................... 433 VOLUME II OF XII TABLES 40 Incidence of Microscopic Observations - Week 8 Sacrifice................................ 474 41 Incidence of Microscopic Observations - Week 14 Sacrifice.............................. 486 42 Incidence of Microscopic Observations - Week 53 Sacrifice.............................. 499 43 Incidence of Microscopic Observations - Terminal and Recovery Sacrifices...... 514 44 Incidence of Microscopic Observations - Unscheduled Deaths........................... 537 45 Incidence of Microscopic Observations - All Animals ....................................... 583 8 Covance 6329-212 3M T-6316.1 VOLUME II OF XII CONTENTS (Continued) Page TABLES 46 Incidence of Severity of Selected Microscopic Observations - Week 8 Sacrifice.......................................................................................................... 635 47 Incidence of Severity of Selected Microscopic Observations - Week 14 Sacrifice.......................................................................................................... 641 48 Incidence of Severity of Selected Microscopic Observations - Week 53 Sacrifice.......................................................................................................... 647 49 Incidence of Severity of Selected Microscopic Observations - Terminal and Recovery Sacrifice.......................................................................................... 653 50 Incidence of Severity of Selected Microscopic Observations - Unscheduled Deaths ............................................................................................................ 661 51 Incidence of Severity of Selected Microscopic Observations - All Animals........ 668 APPENDIX 1.............................................................................................................. 677 Protocol Deviations ................................................................................................. 678 VOLUME III OF XII APPENDIX 2.............................................................................................................. 685 Individual Animal Fate Data.................................................................................... 686 Individual Clinical Observations.............................................................................. 711 VOLUME IV OF XII APPENDIX 3............................................................................................................ 1226 Individual Body Weight Data (g)........................................................................... 1227 APPENDIX 4............................................................................................................ 1306 Individual Food Consumption Data (g).................................................................. 1307 Individual Test Material Consumption Data (mg/kg/day)....................................... 1384 APPENDIX 5............................................................................................................ 1456 Individual Clinical Hematology Data..................................................................... 1457 Individual Clinical Chemistry Data........................................................................ 1499 Individual Clinical Urinalysis Data........................................................................ 1541 Individual Urine Chemistry Data ........................................................................... 1583 9 Covance 6329-212 3M T-6316.1 VOLUME V OF XII CONTENTS (Continued) Page APPENDIX 6............................................................................................................ 1599 Individual Anatomic Pathology Data ..................................................................... 1600 VOLUME VI OF XII APPENDIX 6 (Continued) ........................................................................................ 2094 Individual Anatomic Pathology Data ..................................................................... 2095 VOLUME VII OF XII APPENDIX 6 (Continued) ........................................................................................ 2621 Individual Anatomic Pathology Data ..................................................................... 2622 VOLUME VIII OF XII APPENDIX 6 (Continued) ........................................................................................ 2958 Individual Anatomic Pathology Data ..................................................................... 2959 APPENDIX 7............................................................................................................ 3299 Cell Proliferation Report ....................................................................................... 3300 APPENDIX 8............................................................................................................ 3338 Electron Microscopy Report .................................................................................. 3339 VOLUME IX OF XII APPENDIX 9............................................................................................................ 3380 Covance 6329-228................................................................................................. 3381 10 ABSTRACT Covance 6329-212 3M T-6316.1 The purpose of this study was to assess the carcinogenicity of the test material, N-Ethyl Perfluorooctanesulfonamido Ethanol (N-EtFOSE), when administered in the diet to rats for at least 104 weeks. Male and female Crl:CD(SD)IGS BR rats were assigned to seven groups (70 animals/sex/group for Groups 1, 4, and 5; 60 animals/sex/group for Groups 2 and 3, and 40 animals/sex/group for Groups 6 and 7). Each group received diets containing the vehicle, acetone, (Group 1), or 3 (Group 2), 30 (Group 3), 100 (Groups 4 and 6), or 300 (Groups 5 and 7) ppm N-EtFOSE. Group 6 was placed on recovery after 52 weeks of treatment. Five animals/sex/group in Groups 1 through 5 were sacrificed during Week 4 and livers were collected. Five animals/sex/group in Groups 1 and 5 were sacrificed during Week 8. Five animal/sex/group in Groups 1 through 4 were sacrificed during Week 14. During Week 52, five animals/sex/group in Group 1 and 10 animals/sex in Group 4 were necropsied. The remaining animals in Groups 1 through 4 were sacrificed at the terminal sacrifice during Week 105. The remaining animals in Group 6 were sacrificed during Week 105 after 52 weeks of recovery. Animals that died on test or were sacrificed at unscheduled intervals were also necropsied, but organ weights were not recorded. After 7 weeks of treatment at 300 ppm, three males were found dead or sacrificed in a moribund condition. There was a 20% or greater difference in body weights for males and females given 300 ppm compared to the control values. It was decided that 300 ppm exceeded the maximum tolerated dose and Groups 5 and 7 were terminated during Week 8. An ancillary study was conducted under Covance 6329-228. Seventy animals/sex/group were assigned to two groups, Groups 8 and 9. Animals received diets containing the vehicle, acetone, or 1 ppm N-EtFOSE, respectively. The complete report is presented in Appendix 9, Volume VIII. Clinical observation, body weight, and food consumption data were recorded. Blood and urine samples were collected for hematology, coagulation, clinical chemistry, urinalysis, and urine chemistry tests at specified time points. Selected organs were weighed at the interim sacrifices during Weeks 8, 14, and 53. Adrenals, brain, eyes, kidneys, liver, 11 Covance 6329-212 3M T-6316.1 mesenteric lymph nodes, pancreas, spleen, testes, and ovaries from the animals necropsied at the Week 8 and 14 interim sacrifices were examined microscopically. Microscopic examinations were done on tissues from each animal in Groups 1 and 4 sacrificed at the Week 53 interim sacrifice and terminal sacrifice, and any animals that died on test or were sacrificed at unscheduled intervals. Lesions, liver, lungs, kidneys, pancreas, thyroid, testes, mammary glands (females) and urinary bladder from each animal in Groups 2, 3, and 6 sacrificed at the terminal or recovery sacrifices were examined microscopically Additional liver samples were also collected for evaluation of palmitoyl-CoA oxidase activity and proliferation cell nuclear antigen from five animals/sex/group in Groups 1 through 5 during Week 4, five animals/sex/group in Groups 1 and 5 during Week 8, and five animals/sex/group in Groups 1 through 4 during Week 14. Liver sections from five animals/sex/group from Groups 1 and 4 were stained with Oil Red "O" and examined microscopically. In addition, liver samples from five animals/sex/group were processed and evaluated by electron microscopy. Thirty-six control males and 37 control females, 28 males and 37 females given 3 ppm, 28 males and 35 females given 30 ppm, 33 males and 30 females given 100 ppm (Group 4) for 104 weeks, and 30 males and 28 females given 100 ppm for 52 weeks (Group 6) died on test or were sacrificed in a moribund condition. There were no test material-related effects on mortality. The most frequent cause of death was pituitary adenomas. Clinical observations (thin and hunched appearance, hypoactivity, red discharge from the nose and eyes, few/no/liquid feces, ataxia, squinted eyes, recumbent behavior, and rough/brown/red hair coat) were similar in nature and in the total number of affected animals in all dose groups. There was a dose-related effect on body weights for males and females given 30 or 100 ppm. After 52 weeks of treatment, mean body weights for males given 3, 30, or 100 ppm were 102, 94, or 88% of control values, respectively. Mean body weights for females given 3, 30, or 100 ppm were 94, 89, or 74% of control values, respectively. After 104 weeks of treatment, mean body weights for males given 3, 30, or 100 ppm were 98, 96, and 87% of control values, respectively. Mean body weights for females 12 Covance 6329-212 3M T-6316.1 given 3, 30, or 100 ppm were 107, 109, and 82% of control values, respectively. The body weight changes seen at 100 ppm were considered of toxicological significance. Mean cumulative body weight change was statistically significantly lower for males given 30 or 100 ppm diets for Weeks 1 through 15 and 15 through 29. Mean body weight change was statistically significantly lower for females given 30 or 100 ppm for Weeks 1 through 15 and for Weeks 29 to 57 and for females given 100 ppm for Weeks 15 through 29. There was an increase in the rate of body weight gain for Group 6 animals after they were placed on recovery after 52 weeks of treatment. Mean food consumption for males and females fed the 100 or 300-ppm diets as well as the males fed the 30-ppm diet was generally lower compared with that of the control group, and these values were frequently statistically significantly different from control values. Mean food consumption for males fed the 3-ppm diets and females fed the 3- or 30-ppm diets were comparable to controls with only sporadic differences in mean food consumption noted. The changes seen in food consumption at 100 ppm were considered toxicologically significant and in general correlated with the changes in body weight. The amount of test material consumed by the animals on a mg/kg of body weight/day basis was as follows. Group 2 3 4 5/7 Target Dose Levels (ppm) 3 30 100 300 Achieved Dose Levels - Range (mg/kg body weight/day) Males Females 0.259 - 0.081 0.109 - 0.307 0.860 - 2.618 1.029 - 3.070 3.065 - 8.724 4.213 - 10.166 15.803- 25.408 19.568 - 29.270 Dietary administration of N-EtFOSE for approximately 52 weeks was associated with mildly decreased red blood cell count, hemoglobin, and hematocrit for females given 100 ppm; mildly lower glucose and mildly higher urea nitrogen for males and females given 100 ppm (predominantly at Weeks 14 and 27); moderately higher albumin and moderately lower globulin for males given 100 ppm (predominantly at Weeks 27 and 53); and moderately to markedly lower cholesterol for males given 30 or 100 ppm and females given 100 ppm. The effect on cholesterol was reversed following the 52-week recovery. Administration of N-EtFOSE at 3 ppm for approximately 52 weeks had no effects on clinical pathology test results. Administration of N-EtFOSE for approximately 104 weeks was associated with moderately lower cholesterol for males 13 Covance 6329-212 3M T-6316.1 given 30 or 100 ppm and moderately lower triglycerides for males and females given 100 ppm. At a dose level of 300 ppm for approximately 8 weeks, administration of N-EtFOSE was associated with many effects on clinical pathology results including lower red blood cell count, hemoglobin, hematocrit, glucose, globulin, and cholesterol and higher urea nitrogen, albumin, total bilirubin (males only), aspartate aminotransferase (males only), alanine aminotransferase, and hepatic palmitoyl-CoA oxidase (also observed at Week 4). Most of the effects were moderate to marked in magnitude. The percentage of proliferating hepatocytes (PI) was significantly increased only in males exposed to 300 ppm of N-EtFOSE at 4 weeks on study. This increase was not observed after 8 weeks on study at which time the 300 ppm dose group was removed from the study. The NOEL for hepatocellular proliferation in males was the 100 ppm dose group. Cell proliferation was not statistically significant in females at 4, 8, or 14 weeks on study in any dose group. At the interim sacrifices, body weights were lower and absolute and relative liver weights were higher for animals given 100 or 300 ppm of the test material. Macroscopically, diffusely dark livers were only observed for animals given 100 ppm of the test material. Enlargement, mottling, and masses of the liver were slightly more frequent for animals given 100 ppm than for the controls. Administration of the test material caused an increase in cystic degeneration, hepatocellular hypertrophy, hepatocellular vacuolation, necrosis and hemorrhage of the liver. At the Week 8 sacrifice, hepatocellular hypertrophy, necrosis and hemorrhage were observed for animals given 300 ppm. Hepatocellular hypertrophy was observed only at the Week 14 sacrifice for animals given 30 or 100 ppm, and hepatocellular vacuolation and hypertrophy were present at the Week 53 sacrifice only for animals given 100 ppm. The hepatic changes were considered toxicologically important. Test material related hepatic findings for animals given 100 ppm at the terminal sacrifice were cystic degeneration (males only) and hepatocellular hypertrophy and vacuolation. 14 Covance 6329-212 3M T-6316.1 The incidence of hepatocellular adenomas was slightly higher for males and females given 100 ppm. The higher incidence was statistically significant for females. The only hepatocellular carcinoma in the study was present for a terminal sacrifice female given 100 ppm. Liver samples were obtained from each of 20 animals (10/sex/group from the control and mid-high dose groups) during necropsy at terminal sacrifice for examination by transmission electron microscopy (TEM). Liver samples were assessed for changes that may likely occur due to chronic toxicity of the test material. Electron microscopic evaluation identified mild to moderate changes related to treatment. Mild to moderate smooth endoplasmic reticulum hyperplasia and minimal to mild hepatocellular hypertrophy were the prominent features found to be different between the mid-high dose group and control animals. Other changes included a slight increase in the amount of lipid accumulation and minor increases in lipofuscin were found in the treated animals compared with controls. Male and female Crl:CD(SD)IGS BR rats were administered N-Ethyl Perfluorooctanesulfonamido Ethanol at doses of 0, 3, 30 and 100 ppm at least 104 weeks. There were no significant effects on survival. In males, combined thyroid follicular cell adenoma/carcinoma was significantly increased as compared to controls at 100 ppm (p = 0.011) which was due primarily to an increase in follicular cell adenoma (p = 0.011). Thyroid follicular cell carcinoma alone was not increased. The relationship of this finding to treatment is questionable for several reasons: thyroid follicular cell tumors in historical controls from this laboratory are relatively common (occur with an incidence of greater than 1%), however, no thyroid follicular cell tumors occurred in male controls from this study, there was no evidence on nonneoplastic lesions of the thyroid, the increase at 100 ppm was within the range of historical controls, and a clear dose-response relationship was not present. In females, the incidence of combined hepatocellular adenoma/carcinoma was significantly increased in the 100 ppm dose group as compared to controls (p = 0.007) as a result of a significant increase in hepatocellular adenoma in this group (p = 0.012). There was no increase for trend or pairwise for hepatocellular carcinoma alone. The only hepatocellular carcinoma observed in this study was in a 100-ppm, terminal-sacrifice female. Recovery females in the 100 ppm dose group experienced a marginally significant increase in hepatocellular adenoma (p = 0.05). It is noteworthy that , in females, liver effects were only observed at 100 ppm and included the finding of a 15 Covance 6329-212 3M T-6316.1 hepatocellular adenoma at the Week 53 sacrifice. Significant negative trends and pairwise decreases at 100 ppm occurred among females for mammary tumors (fibroadenoma, combined fibroadenoma/adenoma, and combined fibroadenoma/adenoma/carcinoma). Trends for pituitary adenoma and combined adenoma/carcinoma were also negative in females. 16 PURPOSE Covance 6329-212 3M T-6316.1 The purpose of this study was to assess the carcinogenicity of the test material, N-Ethyl Perfluorooctanesulfonamido Ethanol (N-EtFOSE), when administered in the diet to rats for at least 104 weeks. REGULATORY COMPLIANCE All aspects of this study were conducted in compliance with the Food and Drug Administration Good Laboratory Practice Regulations as set forth in Title 21 of the United States Code of Federal Regulations, Part 58, issued December 22, 1978 (effective June 20, 1979), and with any applicable amendments. The protocol was reviewed and approved by the Institutional Animal Care and Use Committee at Covance. TEST MATERIAL, VEHICLE, AND CARRIER Test Material The test material, T-6316 (narrow range N-ethyl perfluorooctanesulfonamido ethanol, N-EtFOSE), a blend of Lot Nos. 30035, 30037, and 30039, is a waxy solid and is 98.1% N-EtFOSE (w/w) (see Protocol Deviations for exceptions). It was received at Covance on June 20 and July 21, 1997. To avoid repeated heating and sublimation, the two shipments of test material were heated, combined, and predetermined quantities of test material were aliquoted into polyethylene (LDPE) containers. The test material was stored at room temperature. Information on synthesis methods, stability, composition, or other characteristics that define the test material was provided by the Sponsor and is on file at with the Covance. Vehicle The vehicle was acetone (Spectrum Chemical Mfg. Corp., New Brunswick, New Jersey and Gardena, California), Lot Nos. LW0044 (99.96% pure), LH0253 (99.8% pure), and 17 Covance 6329-212 3M T-6316.1 NS0231 (99.8% pure). The shipments were received at Covance on April 9 and June 23, 1997, and September 8, 1998, respectively. The vehicle was stored at room temperature. Information on synthesis methods, composition, or other characteristics that define the acetone is on file with the manufacturer. Carrier Certified rodent diet (#5002 meal, LabDiet by Purina Mills, Inc.) was used as supplied. The lot numbers are recorded in the data. The diet is routinely analyzed by the manufacturer for nutritional components and environmental contaminants. Reserve (Archive) Samples A reserve sample (5 g) of the test material was taken and stored at room temperature. This sample was transferred to the Sponsor after completion of the inlife phase. Disposition The remaining test material was returned to the Sponsor on August 8, 2000. TEST ANIMALS AND HUSBANDRY Animals Male and female Crl:CD(SD)IGS BR rats were obtained from the Raleigh, North Carolina, facility of Charles River Laboratories on January 6, 1998. At initiation of treatment, the animals were 45 to 51 days old; the males weighed from 202 to 310 g (see Protocol Deviations for exceptions), and the females weighed from 133 to 194 g. Identification Each animal was assigned a temporary number upon arrival. Before initiation of treatment, a microchip identification device was implanted into each animal. After randomization for placement on test, each animal was assigned a permanent number, and 18 Covance 6329-212 3M T-6316.1 the microchip was coded with the permanent number. All data for an animal are recorded under these numbers. Justification Historically, rats have been used in safety evaluation studies and are recommended by appropriate regulatory agencies. Husbandry Animal Room 3045 was used for this study. Environmental controls for the animal room were set to maintain 18 to 26oC, a relative humidity of 30 to 70%, a 12-hour light/12-hour dark cycle, and a minimum of 10 room air changes/hour. The animals were housed individually (except for the first 6 days of acclimation when animals were group-housed) in stainless-steel cages. Some animals were placed in polycarbonate cages during the study when health problems dictated. Certified rodent diet (#5002 meal, LabDiet by Purina Mills, Inc.) was provided ad libitum during acclimation. Water was provided ad libitum (see Protocol Deviations for exceptions). Samples of the water are analyzed for specified microorganisms and environmental contaminants. The results are on file with Covance-Madison. There were no known contaminants in the diet or water at levels that would have interfered with this study. Animals not selected for the study (45 males and 41 females) were used for training procedures. Two females were found dead during acclimation. Acclimation Four hundred fifty-five males and 453 females were received on January 6, 1998, and acclimated in Animal Room 3045 for 20 days before initiation of treatment. In general, animals in this shipment appeared healthy. During acclimation, the animals were 19 Covance 6329-212 3M T-6316.1 examined for abnormalities indicative of health problems and body weights were recorded for all animals at randomization. PROCEDURES This study was conducted in accordance with the Protocol dated January 21, 1998, and Protocol Amendment Nos. 1 through 8. Protocol deviations are in Appendix 1. An ancillary study, Covance 6329-228, was conducted in accordance with its protocol, April 1, 1998 and Protocol Amendment Nos. 1 through 5. The complete report is presented in Appendix 9. Group Designations and Dose Levels The animals used for this study were examined by a laboratory animal veterinarian on January 9, 1998, and found to be suitable for study consideration. Selection of animals for the study was based on acclimation data. Animals were assigned to treatment groups using a computerized blocking procedure designed to achieve body weight balance with respect to treatment group. At the time of randomization, the weight variation of the animals did not exceed +2 standard deviations of the mean body weight for each sex. Group mean body weights were analyzed using Levene's test for homogeneity of variance at the 5.0% probability level and found to be homogeneous. Animals were assigned to the study according to the following design. 20 Covance 6329-212 3M T-6316.1 Group 1 (Control)b,c,d,e 2 (Low)c 3 (Mid)c 4 (Mid-High)c,e 5 (High)c,d 6 (Mid-High Recovery)f 7 (High Recovery)d No. of Animals Male Female 70 70 60 60 60 60 70 70 70 70 40 40 40 40 Dietary Levels (ppm N-EtFOSE)a 0 3 30 100 300 100 300 a T-6316 is 98.1% n-ethyl perfluorooctanesulfonamido ethanol N-EtFOSE); dose levels are expressed as ppm of N-EtFOSE. b The control animals received the basal diet/acetone only. c Five animals/sex/group in Groups 1 through 5 were sacrificed during Week 4, and five animals/sex/group in Groups 1 through 4 were sacrificed during Week 14 for hepatocellular proliferation rate measurements and biochemical analyses (palmitoyl-CoA oxidation). d Five animals/sex/group in Groups 1 and 5 were sacrificed during Week 8; the remaining animals in Groups 5 and 7 were sacrificed and discarded during Week 8. e Five animals/sex in Group1 and 10 animals/sex in Group 4 were designated as interim sacrifice animals and were sacrificed after at least 52 weeks of treatment. f Animals in Group 6 were treated for at least 52 weeks, then treatment was discontinued, and the animals were observed for reversibility, persistence, or delayed occurrence of toxic effects for at least 52 weeks posttreatment. During recovery, the animals received basal diet only. Dosing Procedures Dose Preparation. Dietary concentrations were based on the test material as supplied. Diets were prepared at least once every 4 weeks. Preparation of Diet for Group 1. The appropriate amount of certified diet was weighed and mixed with an appropriate volume of acetone. Preparation of Stock. A stock preparation was made using the required amount of test material dissolved in the required volume of acetone. Diets for Groups 4/6 and 5/7 (Base Diet). For the 300-ppm base diet, a premix consisting of a portion of the certified diet with the required amount of the stock preparation was mixed using a Hobart mixer. For the 100-ppm diet, a premix consisting of a portion of the certified diet, the required amount of the stock preparation, 21 Covance 6329-212 3M T-6316.1 and additional acetone was mixed using a Hobart mixer. These premixes were transferred to a mixer with additional certified diet and mixed thoroughly. Diet for Group 2. For the 3-ppm diet, a premix consisting of a portion of the certified diet, the required amount of the prepared 300-ppm diet, and the required volume of acetone, was mixed and then transferred to a mixer with additional certified diet and mixed thoroughly. Diet for Group 3. For the 30-ppm diet, the required amount of the prepared 300-ppm diet and the required volume of acetone were mixed thoroughly with the appropriate amount of certified feed. Samples for dose analyses were taken directly from the mixing bowl. The diets were stored at room temperature (see Protocol Deviations for exceptions) in covered containers until dispensed into feeding jars. Method of Administration. Dietary admixture was used because the potential route of exposure in humans is oral. The dose preparations were administered ad libitum (see Protocol Deviations for exceptions). Animals in Groups 1 through 4 received the test diet for at least 104 weeks (up to Day 732, Week 105, as applicable). Animals in Group 6 received the test diet for 52 weeks. After 7 weeks of treatment at 300 ppm, three males were found dead or were sacrificed in a moribund condition. There was a 20% or greater difference in body weights for males and females given 300 ppm compared to control values. It was decided that 300 ppm exceeded the maximum-tolerated dose and Groups 5 and 7 were terminated during Week 8. Retention Samples. Samples (approximately 100 g) were taken from each dose preparation and stored at room temperature (see Protocol Deviations for exceptions). Unless used for analyses, retention samples from the treated groups were discarded at least 1 month after completion of the inlife phase (see Protocol Deviations for exceptions). Samples from the control diets prepared for Weeks 1 through 24 were shipped to the Sponsor. 22 Covance 6329-212 3M T-6316.1 Dose Analysis Analyses for the concentration of test material in the dose preparations were done by Covance using an analytical method, MP-M312-MA, validated by Covance. Homogeneity was determined for all concentrations once pretest and for preparations for Weeks 1 through 3; for two additional pretest high-dose level preparations; and for four additional pretest low-dose level preparations. One sample (approximately 100 g) taken from the top, middle, and bottom of the mix were collected, divided into three subsamples for extraction and analysis (see Protocol Deviations for exceptions), and analyzed for test material content. All samples were stored at room temperature. To evaluate the stability of the test material in the carrier, four sets of samples (approximately 100 g each) were taken from the low- and high-dose level concentrations of diet preparations mixed pretest. One set was analyzed on the day of mixing; one set was analyzed after storage at room temperature for 19 days; one set was analyzed after storage at room temperature for 34 days; and the remaining set was analyzed after being stored for 8 weeks in a freezer set to maintain -10 to -30oC. In addition, samples (approximately 100 g each) were taken from the low- and high-dose level concentrations of diet preparations mixed pretest. The samples were stored at room temperature for 5 days, then for 7 days under animal room conditions (room temperature in a feeding container), then analyzed. For dose confirmation, samples (approximately 100 g each) from all dose preparations were analyzed in duplicate. Homogeneity samples collected from the middle of the dose preparations for Weeks 1 through 3 were used for dose confirmation results. All samples were stored at room temperature until analyzed. Observation of Animals Clinical Observations. The animals were observed twice daily (a.m. and p.m.) for mortality and moribundity (see Protocol Deviations for exceptions). Once prior to treatment and weekly thereafter, each animal was removed from its cage and examined; abnormal findings or an indication of normal were recorded (see Protocol Deviations for exceptions). In addition, the time of onset, location, size, appearance, and progression of each grossly visible or palpable mass were recorded. 23 Covance 6329-212 3M T-6316.1 Body Weights. Individual body weight data were recorded on Day -4 (randomization), weekly for Weeks 1 through 17, once every 4 weeks thereafter, and at Week 105 (see Protocol Deviations for exceptions). Body weights were also recorded for animals in Groups 5 and 7 before sacrifice during Week 8. Food Consumption. Individual food consumption data were recorded weekly for Weeks 1 through 16 and once every 4 weeks thereafter (see Protocol Deviations for exceptions). Clinical Pathology Blood and urine samples were collected from five animals/sex/group from Groups 1 and 5 during Week 8 and from 10 animals/sex/group from Groups 1 through 4 during Weeks 14, 27, and 53. Blood films were made and held for possible future examination from animals sacrificed at unscheduled intervals and from animals at scheduled sacrifices after at least 52 and 104 weeks of treatment (see Protocol Deviations for exceptions). In addition, blood was collected for cholesterol and triglycerides tests only from all remaining animals at the terminal and recovery sacrifices. Animals were fasted overnight, and urine was collected chilled for approximately 16 hours before blood sampling; water was provided ad libitum. Blood was collected from a jugular vein. The anticoagulant was potassium EDTA for the hematology tests. Samples for clinical chemistry and serum samples were collected without anticoagulant. Animals were bled in random order. The following were evaluated. Hematology red blood cell (erythrocyte) count hemoglobin hematocrit white blood cell (leukocyte) count differential blood cell count segmented neutrophil count mean corpuscular volume mean corpuscular hemoglobin mean corpuscular hemoglobin concentration lymphocyte count monocyte count eosinophil count basophil count platelet count blood cell morphology Reticulocyte smears were made and held for possible future examination. 24 glucose urea nitrogen creatinine total protein albumin globulin cholesterol total bilirubin Covance 6329-212 3M T-6316.1 Clinical Chemistry alanine aminotransferase gamma glutamyltransferase aspartate aminotransferase calcium inorganic phosphorus sodium potassium chloride appearance volume specific gravity pH protein urobilogen Urinalysis glucose ketones bilirubin blood microscopic examination of sediment sodium potassium Urine Chemistry 16-hour excretion of: sodium potassium Serum samples not used for clinical chemistry were stored in a freezer set to maintain -60 to -80oC. Samples were shipped on dry ice to the Sponsor for possible future analysis. Serum Analyses Blood [approximately 2 mL at the Week 4, 8, and 14 sacrifices and approximately 3 mL at the Week 53 and 105 sacrifices (see Protocol Deviations for exceptions)] was collected. Samples were collected from five animals/sex/group in Groups 1 through 5 during Week 4 (animals selected for hepatocellular proliferation and biochemical analyses); five animals/sex/group in Groups 1 and 5 during Week 8; five animals/sex/group in Groups 1 through 4 during Week 14 (animals selected for hepatocellular proliferation and biochemical analyses); five animals/sex/group from Groups 1 and 4 (from animals selected for interim sacrifice) after at least 52 weeks of treatment; and all remaining animals at terminal and recovery sacrifices. 25 Covance 6329-212 3M T-6316.1 Animals were fasted overnight and blood was collected without anticoagulant from a jugular vein. Blood samples were allowed to clot at room temperature, then centrifuged. Serum samples were harvested and stored in a freezer set to maintain -60 to -80oC. Samples were shipped on dry ice to the Sponsor for possible future analyses. Anatomic Pathology Necropsy. A necropsy was done on each animal that died or was sacrificed at an unscheduled interval. Animals sacrificed before Week 13 were anesthetized with sodium pentobarbital and animals sacrificed during or after Week 13 were anesthetized with carbon dioxide (see Protocol Deviations for exceptions). During Week 4, five animals/sex/group from Groups 1 through 5 were fasted overnight, anesthetized with sodium pentobarbital, weighed, and exsanguinated. The liver was removed and weighed. Representative samples of left lateral, right median, and right lateral lobes and any macroscopic lesions of the liver were collected and preserved in zinc formalin for proliferation cell nuclear antigen (PCNA). Liver samples were also collected for palmitoyl CoA analysis. A sample (approximately 1.5 g) from the remaining portion of the liver was collected from selected animals. Samples were placed on wet ice and transferred to the Sponsor for mitochondrial analysis. Results will not be included in this report. Additional liver samples were stored in a freezer, set to maintain -60 to -80oC, and sent to the Sponsor for possible future analysis. During Week 8, five animals/sex/group from Groups 1 and 5 were fasted overnight, bled for clinical pathology and serum analyses, anesthetized with sodium pentobarbital, weighed, exsanguinated, and necropsied. Liver samples were collected for palmitoyl CoA analysis and PCNA evaluation. Additional liver samples were stored in a freezer, set to maintain -60 to -80oC, and shipped to the Sponsor for possible future analysis. The remaining animals in Groups 5 and 7 were euthanatized with carbon dioxide and the carcasses discarded without necropsy. During Week 14, five animals/sex/group from Groups 1 through 4 were fasted overnight, bled for clinical pathology and serum analyses, anesthetized with carbon dioxide, weighed, exsanguinated, and necropsied. Liver samples were collected for palmitoyl CoA analysis and PCNA evaluation. Additional liver samples were stored in a freezer, set to maintain -60 to -80oC, and shipped to the Sponsor for possible future analysis. 26 Covance 6329-212 3M T-6316.1 After at least 52 weeks of treatment, 5 animals/sex from Group 1 and 10 animals/sex from Group 4 were fasted overnight, anesthetized with carbon dioxide, weighed, exsanguinated, and necropsied. Additional liver samples were stored in a freezer, set to maintain -60 to -80oC, and shipped to the Sponsor for possible future analysis. After 104 weeks of treatment, the remaining animals in Groups 1 through 4 were fasted overnight, then anesthetized with carbon dioxide, weighed, exsanguinated, and necropsied. Additional liver samples were stored in a freezer, set to maintain -60 to -80oC, and shipped to the Sponsor for possible future analysis. After 52 weeks of treatment and 52 weeks without treatment, the remaining animals in Group 6 were fasted overnight, then anesthetized with carbon dioxide, weighed, exsanguinated, and necropsied. Additional liver samples were stored in a freezer, set to maintain -60 to -80oC, and shipped to the Sponsor for possible future analysis. The necropsy included a macroscopic examination of the external features of the carcass; external body orifices; the abdominal, thoracic, and cranial cavities; organs; and tissues. Proliferation Cell Nuclear Antigen Evaluation Liver samples were collected from five animals/sex/group in Groups 1 through 5 during Weeks 4 and 8 and five animals/sex/group in Groups 1 through 4 during Week 14. At the Week 4 collection, representative samples of left lateral, right median, and right lateral lobes of the liver and macroscopic lesions of the liver were collected and preserved in zinc formalin. At the Week 8 and 14 collections, representative samples of the left lateral lobe and any macroscopic lesions of the liver were collected and preserved in zinc formalin. After fixation, each sample of the liver was embedded in paraffin, and the paraffin blocks were shipped to Pathology Associates International. Proliferation cell nuclear antigen (PCNA) evaluation were done on the samples (left lateral lobe only). In addition, sections of the left lateral lobe of the liver were stained with hematoxylin and eosin and examined microscopically. 27 Covance 6329-212 3M T-6316.1 Palmitoyl-CoA Oxidase Tissue Collection and Analyses Liver samples were collected from five animals/sex/group in Groups 1 through 5 during Week 4; five animals/sex/group in Groups 1 through 5 during Week 8; and five animals/sex/group in Groups 1 through 4 during Week 14. A sample (approximately 500 mg) of the right lateral lobe of the liver was also collected from each animal and flash-frozen in liquid nitrogen (see Protocol Deviations for exceptions). The liver tissue was stored in a freezer, set to maintain -60 to -80oC, until analyzed for palmitoyl CoA oxidase activity. Organ Weights. At the interim sacrifices during Weeks 8, 14, and 53, the following organs (when present) were weighed; paired organs were weighed separately (see Protocol Deviations for exceptions). adrenal (2) brain kidney (2) liver lung ovary (2) spleen testis (2) thyroid (2) with parathyroid uterus with cervix Organ-to-body weight percentages and organ-to-brain weight ratios were calculated. Tissue Preservation. The following tissues were collected and preserved in 10% neutral-buffered formalin (see Protocol Deviations for exceptions). adrenal (2) brain cecum cervix colon duodenum epididymis (2) esophagus eye (2) femur with bone marrow (articular surface of the distal end) Harderian gland heart ileum jejunum kidney (2) lesions liver lung with mainstem bronchi lymph node (mesenteric) mammary gland (females only) ovary (2) pancreas pituitary prostate rectum salivary gland [mandibular (2)] sciatic nerve 28 seminal vesicle (2) skeletal muscle (thigh) skin spinal cord (cervical, thoracic, and lumbar) spleen sternum with bone marrow stomach Covance 6329-212 3M T-6316.1 testis (2) thymus thyroid (2) with parathyroid trachea urinary bladder uterus vagina Bone marrow smears from the femur of each animal at scheduled sacrifices were prepared, stained with Wright's stain, and retained for possible examination. In addition, at the terminal and recovery sacrifices, a section of the heart and liver were collected from 10 animals/sex in Groups 1, 3, 4 (terminal sacrifice), and 6 (recovery sacrifice) and preserved in 2.0% paraformaldehyde/2.5% glutaraldehyde in 0.1M phosphate buffer. The tissues were processed and embedded in epoxy blocks for possible future examination by electron microscopy. Histopathology. Tissues (as appropriate) were embedded in paraffin, sectioned, stained with hematoxylin and eosin, and examined microscopically from each animal in Groups 1 and 4 sacrificed at the terminal sacrifice and any animals that died on test or were sacrificed at unscheduled intervals. Tissues (as appropriate) from each animal in Groups 1 and 4 sacrificed at the Week 53 interim sacrifice were embedded in paraffin, sectioned, stained with hematoxylin and eosin, and examined microscopically. Lesions, liver, lungs, kidneys, pancreas, thyroid, testes, mammary glands (females), and urinary bladder from each animal in Groups 2, 3, and 6 sacrificed at the terminal or recovery sacrifices were embedded in paraffin, sectioned, stained with hematoxylin and eosin, and examined microscopically. Adrenals, brain, eyes, kidneys, liver, mesenteric lymph nodes, pancreas, spleen, testes, and ovaries from the animals necropsied at the Week 8 and 14 interim sacrifices were also embedded in paraffin, sectioned, stained with hematoxylin and eosin, and examined microscopically. A pathology peer review was conducted by a pathologist designated by the sponsor. 29 Covance 6329-212 3M T-6316.1 Liver sections from five animals/sex/group from Groups 1 and 4 were stained with Oil Red "O" stain and examined microscopically. Liver sections in epoxy blocks for five animals/sex/group from Groups 1 and 4 were processed and evaluated by electron microscopy by Pathology Associates International (report included in Appendix 8). Statistical Evaluation Levene's test (Levene, 1960) was done to test for variance homogeneity. In the case of heterogeneity of variance at p < 0.05, transformations were used to stabilize the variance. One-way analysis of variance [ANOVA (Winer, 1971)] was used to analyze initial body weights, body weight changes, food consumption, continuous clinical pathology values, and organ weight data. ANOVA was done on the homogeneous or transformed data. If the ANOVA was significant, Dunnett's t-test (Dunnett, 1964) was used for pairwise comparisons between treated and control groups. Group comparisons were evaluated at the 5.0%, two-tailed probability level. Only data collected on or after the first day of treatment were analyzed statistically. Survival and Tumor Analysis Survival. Evaluations of trend and heterogeneity of survival data were performed using the Cox-Tarone binary regression method and the National Cancer Institute (NCI) Life Table Package (Thomas, Breslow, and Gart, 1977). Week 105 was treated as the end of the study in the NCI package for both sexes. Animals that died accidentally and those that were sacrificed at scheduled intervals were censored in the analysis. Continuity corrected one-sided tail probabilities for trend and two-sided tail probabilities for group comparisons were evaluated at the 5.0% significance level. Nonneoplastic Lesions. Nonneoplastic lesions were chosen for statistical analyses if the incidence rate in at least one treated group was increased or decreased by at least two occurrences over the control group. These were analyzed by the Cochran-Armitage test for trend and the Fisher-Irwin exact test for control versus treatment comparisons 30 Covance 6329-212 3M T-6316.1 (Thakur, Berry, and Mielke, 1985). One-sided tail probabilities for trend and group comparisons were evaluated at the 5.0% significance level. Neoplastic Lesions. Neoplastic lesions were chosen for statistical analyses if the incidence rate in at least one treated group was increased or decreased by at least two occurrences over the control group. The incidental tumors (i.e., tumors that were not assigned to be the cause of death of the animals by the study pathologist) were analyzed by logistic regression of tumor prevalence (Dinse and Lagakos, 1983). The logistic regression model included linear and quadratic terms in age (week of death), but the quadratic term was eliminated if there was a lack of convergence. The fatal and palpable (superficial) tumors were analyzed by Cox-Tarone binary regression method, as in the case of survival, using the death time or the first palpation time (as applicable) as the tumor onset time. In the case of any particular tumor type where the study pathologist assigned the tumor in question as being the cause of death of a subset of the animals and the rest of the animals were assumed to be dead of other competing risks, IARC-type (Peto, et al., 1980) cause of death analysis was performed. Specifically, the subset of the tumors, which were assigned to be the cause of death by the examining pathologist, was analyzed by Cox-Tarone logistic regression under life table techniques. The subset, which was considered incidental by the pathologist, was analyzed by logistic regression of tumor prevalences. For the cases of tumor types where cause of death was undetermined, these were treated as incidental for statistical evaluation. The score statistics and their respective variances from the above tests were then used to compute the combined evidence as described by Gart, et al., (1986). In the case of only one tumor belonging to one of the two categories (fatal and incidental) in a test, it was combined with the other category for the purpose of statistical analyses. In addition, in cases where there was lack of convergence for the asymptotic test of the logistic regression method, the exact probability of the test was obtained by using LogXact-Turbo (1993) and combined with the probability of the Cox-Tarone test, if necessary. Common and rare tumors were evaluated at the 1.0% and 5.0% significance levels, respectively, for trend. The benign and malignant neoplastic incidences were evaluated separately, but were combined where appropriate. The criteria for combination were based on the work of McConnell et al. (1986). The incidences of multiple-organ findings such as hemangioma, hemangiosarcoma, lipoma, liposarcoma, fibroma, fibrosarcoma, leiomyoma, leiomyosarcoma, endometrial stromal polyp, and endometrial stromal sarcoma were counted by animal, not by tissue type. They were evaluated statistically if they met the selection criterion for the analysis. The statistical results for these cases may 31 Covance 6329-212 3M T-6316.1 be biased because not all the animals were examined for every tissue. Pairwise one-sided group comparisons were evaluated at the 5.0% significance level. Groups 5 (High-dose) and 7 (High recovery) were excluded from all the analyses except the survival graphs due to early sacrifices at Week 8. In the nonneoplastic analyses, animals sacrificed early (Week 4) in Groups 1 through 4 (Control, Low, Mid, and Mid-High, respectively) were excluded from the denominators. In the statistical evaluations of the neoplastic lesions, animals sacrificed early (during or before Week 14) in Groups 1 through 4 were discounted from the number of animals at risk. Further, in the neoplastic analyses, in the cases where the low, mid, and mid-high recovery groups did not have a complete histopathology examination, they were excluded from statistical analyses. The p-values under Group 1 (Control) are for trend analysis and the p-values under Groups 2 (Low), 3 (Mid), 4 (Mid-High), and 6 (Mid-High recovery) are for the comparison of that group versus the control group. Group 6 was also compared with Group 4. Trend analysis includes Groups 1, 2, 3, and 4 only. Ordinal dose levels 0, 1, 2, and 3 were used in all analyses for Groups 1, 2, 3, and 4, respectively. Continuity correction was done for all asymptotic tests. The following notations are used throughout this report to designate direction and significance of effects. + = Effect in the increased direction - = Effect in the decreased direction * = Significant at p < 0.05 ** = Significant at p < 0.01 E = Exact permutation test NA = Not analyzed Record Retention The raw data, documentation, records, specimens, protocol, and final report generated as a result of this study will be archived in the storage facilities of Covance-Madison. At least 1 year after submission of the final report, the Covance Archives staff will contact the Sponsor. At that time, the Sponsor may choose to have the aforementioned materials returned, archived for an additional period of time, or disposed of by Covance; a fee will 32 Covance 6329-212 3M T-6316.1 be charged based on the archive disposition option selected by the Sponsor. Raw data stored on magnetic media and the protocol, study correspondence, and the original final report will be retained by Covance. At least 1 year after submission of the final report, any frozen samples will be returned to the Sponsor, retained by Covance, or disposed of, and a fee will be charged. Data generated by the Sponsor will be archived in the Sponsor's storage facilities. Specimens, data, and reports for PCNA evaluation and electron microscopy will be retained by Pathology Associates International. Frozen liver samples and serum samples sent to the Sponsor will be retained by the Sponsor. RESULTS AND DISCUSSION Dose Analysis Results of the homogeneity, stability, and dose preparation analyses are in Tables 1 through 3. Prestudy (September 11, 29, October 15, and November 7 and 13, 1997), several homogeneity analyses were conducted on each of several mixes in an attempt to resolve issues related to the inherent variability and lack of sufficient sensitivity at low levels in the analytical method for measuring N-EtFOSE. Mean values of the homogeneity analyses for the mix done on January 15, 1998, ranged from 89.7-104%, 92.0-98.7%, 86.8-98.4%, and 94.0-96.7% of the theoretical concentrations for the diets containing 3, 30, 100, or 300 ppm N-EtFOSE, respectively. Mean values of an additional homogeneity analyses for the mix done on August 26, 1999, ranged from 66.2 to 72.7% and 121 to 136% of the theoretical concentrations for the diets containing 100 or 300 ppm, respectively. Prestudy (September 11 and 29, 1997), several stability analyses were conducted on each of several mixes in an attempt to resolve the issues related to the inherent variability and lack of sufficient sensitivity at low levels in the analytical method for measuring N EtFOSE. Results of the stability analyses for the mix conducted on October 15, 1997, of samples stored for 19 days at room temperature conditions indicated that the mean concentrations were 99.3 and 86.7% of the theoretical concentrations of 3 and 300 ppm, respectively. Results of the stability analyses for the mix conducted on October 15, 1997, of samples stored for 34 days at room temperature conditions indicated that the mean concentrations were 100 and 88.7% of the theoretical concentrations of 3 and 300 ppm, respectively. Results of stability analyses for the mix conducted on October 15, 1997, of 33 Covance 6329-212 3M T-6316.1 samples stored for 8 weeks under frozen conditions indicated that the mean concentrations were 99.0 and 94.3% of the theoretical concentrations of 3 and 300 ppm, respectively. Results of the stability analyses for an additional mix conducted November 13, 1997, of samples stored 5 days at room temperature followed by 7 days under animal room conditions indicated that the mean concentrations were 118 and 87.7% of the theoretical concentrations of 3 and 300 ppm, respectively. The mean concentrations of the dose preparation analyses for all levels ranged from 33.3 to 158% of the theoretical concentrations (including the reassay and retention sample analyses, but excluding data that required reanalysis.). Inherent variability and lack of sufficient sensitivity at low levels in the analytical method for measuring N-EtFOSE resulted in homogeneity, stability, and routine analyses data that were in many cases outside of the standard limits of +15%. Observation of Animals Survival and Clinical Observations. Clinical observations including mass observations are summarized in Tables 5 and 6; individual data are in Appendix 2. Survival data are illustrated in Figures 1 and 2 and summarized in Table 4; individual animal fate data are in Appendix 2. Thirty-six control males and 37 control females, 28 males and 37 females given 3 ppm, 28 males and 35 females given 30 ppm, 33 males and 30 females given 100 ppm for 104 weeks (Group 4) and 30 males and 28 females given 100 ppm for 52 weeks (Group 6) died on test or were sacrificed in a moribund condition. There were no test material-related effects on mortality. The most frequent cause of death was pituitary adenoma. Clinical observations were similar in nature and in the total number of affected animals in all groups. Body Weights. Body weight and body weight change data are illustrated in Figures 3 and 4 and summarized in Tables 7 and 8; individual body weight data are in Appendix 3. There was a dose-related decrease on body weights for males and females given 30 or 100 ppm. Mean cumulative body weight change was statistically significantly lower for males given 30 or 100 ppm diets for Weeks 1 through 15 and 15 through 29. Mean body 34 Covance 6329-212 3M T-6316.1 weight change was statistically significantly lower for females given 30 or 100 ppm for Weeks 1 through 15 and for Weeks 29 to 57 and for females given 100 ppm for Weeks 15 through 29. There was an increase in the rate of body weight gain for Group 6 animals after they were placed on recovery after 52 weeks of treatment. Food Consumption. Food consumption data are illustrated in Figures 5 and 6 and summarized in Table 9; individual data are in Appendix 4. Mean food consumption for males and females fed the 100- or 300-ppm diets as well as the males fed the 30-ppm diet was generally lower compared with that of the control group, and these values were frequently statistically significantly different from control values. Mean food consumption for males fed the 3-ppm diets and females fed the 3- or 30-ppm diets were comparable to controls with only sporadic differences in mean food consumption noted. Test Material Consumption. Test material consumption data are summarized in Table 10. Individual test material consumption data are presented in Appendix 4. Animals were fed diets containing 3, 30, 100, or 300 ppm N-EtFOSE The amount of test material consumed by the animals on a mg/kg of body weight/day basis was as follows. Group 2 3 4 5/7 Target Dose Levels (ppm) 3 30 100 300 Achieved Dose Levels - Range (mg/kg body weight/day) Males Females 0.259 - 0.081 0.109 - 0.307 0.860 - 2.618 1.029 - 3.070 3.065 - 8.724 4.213 - 10.166 15.803- 25.408 19.568 - 29.270 Clinical Pathology Hematology, coagulation, clinical chemistry, urinalysis, and urine chemistry data are summarized in Tables 11 through 28; individual data are in Appendix 5. The Pathology Report contains a discussion of the data. Dietary administration of N-EtFOSE for approximately 52 weeks was associated with mildly decreased red blood cell count, hemoglobin, and hematocrit for females given 100 ppm; mildly lower glucose and mildly higher urea nitrogen for males and females 35 Covance 6329-212 3M T-6316.1 given 100 ppm (predominantly at Weeks 14 and 27); moderately higher albumin and moderately lower globulin for males given 100 ppm (predominantly at Weeks 27 and 53); and moderately to markedly lower cholesterol for males given 30 or 100 ppm and females given 100 ppm. The effect on cholesterol was reversed following the 52-week recovery. Administration of N-EtFOSE at 3 ppm for approximately 52 weeks had no effects on clinical pathology test results. Administration of N-EtFOSE for approximately 104 weeks was associated with moderately lower cholesterol for males given 30 or 100 ppm and moderately lower triglycerides for males and females given 100 ppm. At a dose level of 300 ppm for approximately 8 weeks, administration of N-EtFOSE was associated with many effects on clinical pathology results including lower red blood cell count, hemoglobin, hematocrit, glucose, globulin, and cholesterol and higher urea nitrogen, albumin, total bilirubin (males only), aspartate aminotransferase (males only), alanine aminotransferase, and hepatic palmitoyl-CoA oxidase (also observed at Week 4). Most of the effects were moderate to marked in magnitude. Anatomic Pathology Organ weight data are summarized in Tables 29 through 32; incidences of macroscopic and microscopic observations are summarized in Tables 33 through 45; the incidence of severity of selected microscopic observations is summarized in Tables 46 through 51. Individual data are in Appendix 6. The Pathology Report contains a discussion of the data. At the interim sacrifices, body weights were lower and absolute and relative liver weights were higher for animals given 100 or 300 ppm of the test material. Macroscopically, diffusely dark livers were only observed for animals given 100 ppm of the test material. Enlargement, mottling, and masses of the liver were slightly more frequent for animals given 100 ppm than for the controls. Administration of the test material caused an increase in cystic degeneration, hepatocellular hypertrophy, hepatocellular vacuolation, necrosis and hemorrhage of the liver. 36 Covance 6329-212 3M T-6316.1 At the Week 8 sacrifice, hepatocellular hypertrophy, necrosis and hemorrhage were observed for animals given 300 ppm. Hepatocellular hypertrophy was observed only at the Week 14 sacrifice for animals given 30 or 100 ppm, and hepatocellular vacuolation and hypertrophy were present at the Week 53 sacrifice only for animals given 100 ppm. The hepatic changes were considered toxicologically important. In males, combined thyroid follicular cell adenoma/carcinoma was significantly increased as compared to controls at 100 ppm (p = 0.011) which was due primarily to an increase in follicular cell adenoma (p = 0.011). Thyroid follicular cell carcinoma alone was not increased. The relationship of this finding to treatment is questionable for several reasons: thyroid follicular cell tumors in historical controls from this laboratory are relatively common (occur with an incidence of greater than 1%), however, no thyroid follicular cell tumors occurred in male controls from this study, there was no evidence on nonneoplastic lesions of the thyroid, the increase at 100 ppm was within the range of historical controls, and a clear dose-response relationship was not present. In females, the incidence of combined hepatocellular adenoma/carcinoma was significantly increased in the 100 ppm dose group as compared to controls (p = 0.007) as a result of a significant increase in hepatocellular adenoma in this group (p = 0.012). There was no increase for trend or pairwise for hepatocellular carcinoma alone. The only hepatocellular carcinoma observed in this study was in a 100-ppm, terminal-sacrifice female. Recovery females in the 100 ppm dose group experienced a marginally significant increase in hepatocellular adenoma (p = 0.05). It is noteworthy that , in females, liver effects were only observed at 100 ppm and included the finding of a hepatocellular adenoma at the Week 53 sacrifice. Significant negative trends and pairwise decreases at 100 ppm occurred among females for mammary tumors (fibroadenoma, combined fibroadenoma/adenoma, and combined fibroadenoma/adenoma/carcinoma). Trends for pituitary adenoma and combined adenoma/carcinoma were also negative in females. Proliferation Cell Nuclear Antigen Results A statistically significant increase in proliferative index was observed transiently in males of the 300 ppm dose group at Week 4, but not at Week 8 or in any other male dose groups. Biological significance for an individual animal is defined as a two-fold or greater increase in proliferative index (PI) over the averaged control values, and the PI must be greater than the highest control value. Biologically significant increases in PI 37 Covance 6329-212 3M T-6316.1 were observed in one out of five males of the 100 ppm dose group at Week 4, however, these increases were not sustained through Week 8. Biologically significant increases in PI were observed in two out of five males at Week 4 and in two out of five males in the 300 ppm dose group at Week 8. No increase in hepatocellular proliferation was observed at Week 14 in the male 100 ppm dose group. The NOEL for hepatocellular proliferation in males was the 100 ppm dose group. Cell proliferation was not statistically or biologically significant in females at 4, 8, or 14 weeks on study in any dose group. The NOEL for hepatocellular proliferation in females was the 300 ppm dose group. Electron Microscopy Results Liver samples were obtained from each of 20 animals (10 animals/sex/group from the control and mid-high dose groups) during necropsy at terminal sacrifice for examination by transmission electron microscopy (TEM). Liver samples were assessed for changes that may likely occur due to chronic toxicity of the test material. Electron microscopic evaluation identified mild to moderate changes related to treatment. Mild to moderate smooth endoplasmic reticulum hyperplasia and minimal to mild hepatocellular hypertrophy were the prominent features found to be different between the mid-high dose group and control animals. Other changes included a slight increase in the amount of lipid accumulation and minor increases in lipofuscin were found in the treated animals compared with controls. CONCLUSION Male and female Crl:CD(SD)IGS BR rats were administered N-Ethyl Perfluorooctanesulfonamido Ethanol at doses of 0, 3, 30 and 100 ppm at least 104 weeks. There were no significant effects on survival, and the incidence of hepatocellular adenomas was slightly higher for males and females given 100 ppm. The only hepatocellular carcinoma in the study was present for a terminal sacrifice female given 100 ppm. 38 SIGNATURES Covance 6329-212 3M T-6316.1 39 Covance 6329-212 3M T-6316.1 REFERENCES Dunnett, C. W., "New Tables for Multiple Comparisons with a Control," Biometrics, 20:482-491 (1964). Levene, H., "Robust Tests for Equality of Variances," Contributions to Probability and Statistics, (eds.) I. Olkin et al., Ch. 25, pp. 278-292, Stanford University Press: Stanford, California (1960). Winer, B. J., "Design and Analysis of Single-Factor Experiments," Statistical Principles in Experimental Design, Second Ed., Ch. 3, pp. 149-260, McGraw-Hill: New York, New York (1971a). Winer, B. J., "Analysis of Covariance," Statistical Principles in Experimental Design, Second Ed., Ch. 10, pp. 752-812, McGraw-Hill: New York, New York (1971b). 40 PATHOLOGY REPORT Covance 6329-212 3M T-6316.1 SUMMARY The purpose of this study was to assess the carcinogenicity of the test material, N-Ethyl Perfluorooctanesulfonamido Ethanol (N-EtFOSE), when administered in the diet to rats for at least 104 weeks. The test material was administered at dose levels of 0, 3, 30, 100, and 300 ppm. Animals given 300 ppm were sacrificed during Week 8. Dietary administration of N-EtFOSE for approximately 52 weeks was associated with mildly decreased red blood cell count, hemoglobin, and hematocrit for females given 100 ppm; mildly lower glucose and mildly higher urea nitrogen for males and females given 100 ppm (predominantly at Weeks 14 and 27); moderately higher albumin and moderately lower globulin for males given 100 ppm (predominantly at Weeks 27 and 53); and moderately to markedly lower cholesterol for males given 30 or 100 ppm and females given 100 ppm. The effect on cholesterol was reversed following the 52-week recovery. Administration of N-EtFOSE at 3 ppm for approximately 52 weeks had no effects on clinical pathology test results. Administration of N-EtFOSE for approximately 104 weeks was associated with moderately lower cholesterol for males given 30 or 100 ppm and moderately lower triglycerides for males and females given 100 ppm. At a dose level of 300 ppm for approximately 8 weeks, administration of N-EtFOSE was associated with many effects on clinical pathology results including lower red blood cell count, hemoglobin, hematocrit, glucose, globulin, and cholesterol and higher urea nitrogen, albumin, total bilirubin (males only), aspartate aminotransferase (males only), alanine aminotransferase, and hepatic palmitoyl-CoA oxidase (also observed at Week 4). Most of the effects were moderate to marked in magnitude. At the interim sacrifices, body weights were lower and absolute and relative liver weights were higher for animals given 100 or 300 ppm of the test material. Macroscopically, diffusely dark livers were only observed for animals given 100 ppm of the test material. Enlargement, mottling, and masses of the liver were slightly more frequent for animals given 100 ppm than for the controls. 41 Covance 6329-212 3M T-6316.1 Administration of the test material caused an increase in cystic degeneration, hepatocellular hypertrophy, hepatocellular vacuolation, necrosis and hemorrhage of the liver. At the Week 8 sacrifice, hepatocellular hypertrophy, necrosis and hemorrhage were observed for animals given 300 ppm. Hepatocellular hypertrophy was observed only at the Week 14 sacrifice for animals given 30 or 100 ppm, and hepatocellular vacuolation and hypertrophy were present at the Week 53 sacrifice only for animals given 100 ppm. The hepatic changes were considered toxicologically important. Test material related hepatic findings for animals given 100 ppm at the terminal sacrifice were cystic degeneration (males only) and hepatocellular hypertrophy and vacuolation. The incidence of hepatocellular adenomas was slightly higher for males and females given 100 ppm. The higher incidence was statistically significant for females. The only hepatocellular carcinoma in the study was present for a terminal sacrifice female given 100 ppm. The incidence of hepatocellular adenomas and carcinomas is listed in Text Table 2. METHODS Seven groups of Crl:CD(SD)IGS BR rats were studied using the following study design. 42 Covance 6329-212 3M T-6316.1 No. of Animals Dietary Levels a Group 1 (Control) b Male 70 Female 70 (ppm N-EtFOSE) 0 2 (Low) 60 60 3 3 (Mid) 60 60 30 4 (Mid-High) 5 (High)c 70 70 100 70 70 300 6 (Mid-High Recovery) 40 40 100 7 (High Recovery)c 40 40 300 8 70 70 0 9 70 70 1 a T-6316 is 98.1% n-ethyl perfluorooctanesulfonamido ethanol (N-etFOSE); dose levels are expressed as ppm of N-EtFOSE. b The control animals received the basal diet only. c Five animals/sex in Group 5 were sacrificed during Week 8; the remaining animals in Groups 5 and 7 were sacrificed and discarded during Week 8. During Week 8, blood and urine were collected for hematology, clinical chemistry, urinalysis, and urine chemistry tests from five animals/sex/group in Groups 1 and 5. During Weeks 14, 27, and 53, blood and urine were collected for these tests from 10 animals/sex/group in Groups 1 through 4. Blood smears were made for all animals sacrificed at unscheduled intervals or during Weeks 53 (interim sacrifice) and 105 (terminal and recovery sacrifices). During Week 105, blood was collected for cholesterol and triglycerides from all surviving animals. Necropsies were performed on five animals/sex in Groups 1 and 5 sacrificed during Week 8; five animals/sex in Groups 1 through 4 sacrificed during Week 14, five animals/sex in Group 1 and 10 animals/sex in Group 4 sacrificed during Week 53, and all remaining surviving animals during Week 105. At necropsy, macroscopic observations were recorded, organ weights were obtained (for sacrifices during Weeks 8, 14, and 53), and tissues were placed in fixative as specified by the protocol. At unscheduled necropsies for animals that died or were sacrificed because of poor health, macroscopic observations were recorded, and tissues were placed in fixative, but organ weights were not obtained. Microscopic examinations were done on selected tissues from animals in Groups 1 through 5 necropsied during Weeks 8 and 14. The tissues were adrenals, brain, eyes, kidneys, liver, mesenteric lymph node, pancreas, spleen, testes, and ovaries. Microscopic examinations were done on all collected tissues from animals in Groups 1and 4 necropsied at the Week 53 interim sacrifice and the Week 105 terminal sacrifice and from animals that died or were sacrificed due to poor health. In addition, 43 Covance 6329-212 3M T-6316.1 microscopic examinations were done on macroscopic lesions, liver, lungs, kidney, pancreas, thyroid, testes, and mammary glands (females) from animals in Groups 2, 3, and 6 sacrificed during Week 105. Five animals/sex in Groups 1 through 5 were sacrificed during Week 4, and five animals/sex in Groups 1 through 4 were sacrificed during Week 14 for hepatocellular proliferation rate measurements and hepatic palmitoyl-CoA oxidase activity. Statistically significant differences cited in the Results and Discussion section are based on comparisons between the control and treated groups. RESULTS AND DISCUSSION Mortality The incidence of mortality during the study was similar for treated and control animals. Thirty-six males and 37 females in the control group, 28 males and 37 females given 3 ppm, 28 males and 35 females given 30 ppm, and 33 males and 31 females given 100 ppm died on test or were sacrificed in moribund condition. Clinical Pathology Week 4. Males and females given 300 ppm had mildly, but statistically, higher hepatic palmitoyl-CoA oxidase activity consistent with peroxisomal proliferation. Week 8. There were many statistically significant or otherwise notable differences for clinical pathology results between animals given the control material and animals given 300 ppm. Those differences considered to be effects of the test material were lower red blood cell count, hemoglobin, hematocrit, glucose, globulin, and cholesterol and higher urea nitrogen, albumin, total bilirubin (males only), aspartate aminotransferase (males only), alanine aminotransferase, and hepatic palmitoyl-CoA oxidase. With the exception of higher alanine aminotransferase for the females, these differences were statistically significant. The effects on the erythrocyte parameters, glucose, and globulin were consistent with poor health and failure to gain weight appropriately. Higher urea nitrogen and albumin were most consistent with mild dehydration and indicated the effects on other parameters (e.g., the erythrocyte parameters) would likely have been greater if the animals were normovolemic. The effects on alanine aminotransferase, aspartate 44 Covance 6329-212 3M T-6316.1 aminotransferase, and total bilirubin were indicative of the hepatocellular degeneration and necrosis observed microscopically. Other statistically significant differences were considered to be of uncertain relationship to administration of the test material. Mildly lower platelet count was observed for males but not females. Absolute eosinophil count was statistically lower for both males and females, but the differences were extremely small as normal eosinophil count is very low. Weeks 14, 27, and 53. There were several statistically significant or otherwise notable differences for clinical pathology results between control and treated animals. Those differences considered to be effects of the test material were not always statistically significant but were generally consistent over time and exhibited a relationship to dose. Several statistically significant differences that were inconsistent over time or did not affect the high-dose group were considered incidental. Administration of the test material was associated with decreased red blood cell count, hemoglobin, and hematocrit for females given 100 ppm; lower glucose and higher urea nitrogen for males and females given 100 ppm (predominantly at Weeks 14 and 27); higher albumin and lower globulin for males given 100 ppm (predominantly at Weeks 27 and 53); and lower cholesterol for males given 30 or 100 ppm and females given 100 ppm. These effects were consistent with those observed at Week 8 for animals given 300 ppm but were less prominent. The effect of greatest magnitude was that for cholesterol. At the highest dose level, cholesterol was markedly decreased for the males. The erythrocyte effects and the effects on glucose and urea nitrogen were very mild, and the serum protein effects were considered of moderate severity. Administration of the test material at 3 ppm for approximately 52 weeks had no effect on clinical pathology test results. Week 105. Cholesterol remained moderately lower for males given 30 or 100 ppm (Groups 3 and 4), and triglycerides were moderately lower for females given 100 ppm (Group 4). These effects were absent or reversed for animals in the recovery group (Group 6). 45 Covance 6329-212 3M T-6316.1 Anatomic Pathology - All Animals - Test Material-Related Findings Macroscopic Findings. Diffusely dark livers were observed only for animals given the test material. Enlargement, mottling, and masses of the liver were slightly more frequent for animals given 100 ppm than for the controls. An increased incidence of light foci of the liver for treated animals was not considered test material-related as most of these were due to tension lipidosis, an incidental finding frequently observed in rats. The incidence of possible test material-related macroscopic findings are listed in Text Table 1. Text Table 1 Incidence of Possible Test Material-Related Macroscopic Findings N-Ethyl Perfluorooctanesulfonamido (ppm) 0 3 30 100 100a 0 3 30 100 100a Males Females Animals Examined 70 60 70 40 70 60 60 70 40 Liver Light Focus (I)/Area(s) 0 4 2 5 4 032 6 6 Large 14 1 4 1 110 3 0 Mottled 53 9 6 1 321 8 4 Diffusely Dark 00 0 5 1 000 7 1 Masses 22 1 3 0 010 3 1 a Animals placed on recovery. Microscopic Findings. Test material-related findings were limited to the liver. Administration of the test material caused an increase in cystic degeneration, hepatocellular hypertrophy, hepatocellular vacuolation, necrosis, and hemorrhage. At the Week 8 sacrifice, hepatocellular hypertrophy, necrosis, and hemorrhage were observed for animals given 300 ppm. Hepatocellular hypertrophy was observed only at the Week 14 sacrifice for animals given 30 or 100 ppm, and hepatocellular vacuolation and hypertrophy were present at the Week 53 sacrifice only for animals given 100 ppm. For the unscheduled sacrifice animals, cystic degeneration, and hepatocellular hypertrophy were increased for males and hepatocellular vacuolation was increased for treated males and females. Hepatocellular adenomas were minimally higher for treated animals compared with the controls. 46 Covance 6329-212 3M T-6316.1 Test material-related hepatic findings for animals given 100 ppm at the terminal sacrifice were cystic degeneration (males only), hepatocellular hypertrophy, and vacuolation. Livers from five males, five females in the control group, and five males and five females given 100 ppm were stained with Oil Red O for lipid. The amount of lipid present was slightly higher for males given 100 ppm than for the control males. The incidence and amount of lipid present for control males was one minimal and three slight and for males given 100 ppm it was one minimal, two moderate, and one moderately severe. There was no difference in the amount of lipid present for the females. The incidence of hepatocellular adenomas was slightly higher for males and females given 100 ppm. There was only one hepatocellular carcinoma in the study, and it was present for a terminal sacrifice female given 100 ppm. The microscopic findings for the Group 6 sacrifice animals given 100 ppm were similar to the controls. However, 2 of the 12 females had hepatocellular adenomas. The incidence of test material-related microscopic findings for all animals are listed in Text Table 2. Text Table 2 Incidence of Test Material-Related Microscopic Findings N-Ethyl Perfluorooctanesulfonamido (ppm) 0 3 30 100 100a 0 3 30 100 100a Males Females Animals Examined 65 55 55 65 40 65 55 55 65 40 Liver Cystic Degeneration 6 8 14 18 7 02 0 2 1 Hepatocellular Hypertrophy 0 1 3 22 2 1 0 0 14 0 Hepatocellular Vacuolation 17 18 23 39 11 13 14 8 26 8 Hepatocellular Adenoma 24 2 5 0 01 3 6 3 Hepatocellular Carcinoma 00 0 0 0 00 0 1 0 a Animals placed on recovery. Anatomic Pathology - All Animals - Nontest Material-Related Findings Macroscopic Findings. The most frequently observed findings at necropsy other than those observed associated with test material-related effects were ventral compression of the brain, pituitary masses, ocular discharge, perineal staining, and malocclusion. Other frequent incidental macroscopic findings for males were red foci in the liver, fluid contents in the gastrointestinal tract, obesity, nodular prostate, and nasal discharge. 47 Covance 6329-212 3M T-6316.1 Frequently observed nontest material-related findings for females were mammary masses, thickened mammary glands, thin animals, ovarian cyst, uterine cyst, stained hair coat, and dark foci in the stomach. Microscopic Findings/Neoplastic Findings. The most frequently observed nontest material-related neoplasms were pituitary adenomas, and c-cell adenomas and follicular cell adenomas of the thyroid. Mammary adenomas, fibroadenomas, and carcinomas were frequently present for females. Other tumors were only present in a few animals, and the only rare tumor in the study was a nephroblastoma of the kidney for one male given 3 ppm. Frequently observed nontest material-related neoplasms are listed in Text Table 3. Text Table 3 Incidence of Frequently Observed Neoplasms Pituitary Adenoma Thyroid C-cell Adenoma Thyroid Follicular Cell Adenoma Mammary Adenoma Mammary Fibroadenoma Mammary Carcinoma N-Ethyl Perfluorooctanesulfonamido (ppm) 0 3 30 100 0 3 30 100 Males Females 65 55 55 65 65 55 55 65 46 28 36 48 48 46 48 45 10 10 10 12 8 13 8 11 0316 1 024 0001 6 463 3 1 0 0 23 21 16 13 0000 9 9 15 11 Microscopic Findings - Nonneoplastic Findings. The most frequently observed incidental nonneoplastic findings were ventral compression of the brain, pituitary hemorrhage, chronic progressive nephropathy, proteinaceous casts of the kidneys, ultimobranchial cysts of the thyroid, pelvic mineralization of the kidneys, alveolar macrophages in the lungs, ventricular dilatation of the brain, and hemorrhage of the adrenal cortex. Frequent nontest material-related microscopic findings for males were chronic active inflammation of the prostate, chronic inflammation of the liver, vacuolation of the adrenal cortex, degeneration and fibrosis of the heart, vascular mineralization of the lungs, acinar atrophy of the pancreas, and erosion, fibrosis, and acute inflammation of foot pads. Other frequently observed incidental microscopic findings for females were mammary galactoceles, angiectasis of the adrenal cortex, ovarian cysts, uterine dilatation, thymic cysts, and bile duct hyperplasia of the liver. 48 Covance 6329-212 3M T-6316.1 Week 4 Sacrifice Organ Weights. Terminal body weights were lower for males given 300 ppm. Absolute and relative liver weights were higher for males and females given 100 or 300 ppm. There were no other test material-related changes for absolute organ weights, organ-to-body weight percentages, or organ-to-brain weight ratios. Macroscopic Findings. There were no gross findings noted at necropsy. Week 8 Sacrifice Organ Weights. Terminal body weights were lower and absolute and relative liver weights were significantly higher for males and females given 300 ppm. There were no other test material-related changes for absolute organ weights, organ-to-body weight percentages, or organ-to-brain weight ratios. Macroscopic Findings. The only test material-related findings at necropsy were large livers for five males and two females given 300 ppm and mottling of the liver for one female given 300 ppm. There were no other test material-related gross findings noted at necropsy. Microscopic Findings. Test material-related findings were limited to the liver. The test material-related hepatic findings were hepatocellular hypertrophy, hepatocellular necrosis, and hemorrhage. The incidence and severity of the hepatic lesions are listed in Text Table 4. 49 Covance 6329-212 3M T-6316.1 Text Table 4 Incidence and Severity of Test Material-Related Microscopic Findings Dose Level Number examined Liver Hypertrophy Moderate Moderately-severe Necrosis Minimal Moderate Hemorrhage N-Ethyl Perfluorooctanesulfonamido (ppm) 0 300 0 300 Males Females 5 5 5 5 0 5 0 4 0 0 0 1 0 1 0 0 0 2 0 1 Minimal 0 3 0 0 Moderate 0 0 0 1 Week 14 Sacrifice Organ Weights. Terminal body weights were lower and absolute and relative liver weights were significantly higher for males and females given 100 ppm. There were no other test material-related changes for absolute organ weights, organ-to-body weight percentages, or organ-to-brain weight ratios. Macroscopic Findings. There were no test material-related gross findings noted at necropsy. Microscopic Findings. The only test material-related finding was hepatocellular hypertrophy for males given 30 or 100 ppm and females given 100 ppm. The incidence and severity of the hepatocellular hypertrophy are listed in Text Table 5. 50 Covance 6329-212 3M T-6316.1 Text Table 5 Incidence and Severity of Hepatocellular Hypertrophy Number examined Liver Hypertrophy Minimal Slight Moderate N-Ethyl Perfluorooctanesulfonamido (ppm) 0 3 30 100 0 3 30 100 Males Females 55 5 5 555 5 01 1 0 000 0 00 1 4 000 2 00 1 1 000 0 Week 53 Sacrifice Organ Weights. Terminal body weights were lower and absolute and relative liver weights were significantly higher for males and females given 100 ppm. There were no other test material-related changes for absolute organ weights, organ-to-body weight percentages, or organ-to-brain weight ratios. Macroscopic Findings. There were no test material-related gross findings noted at necropsy. Microscopic Findings. Hepatocellular hypertrophy and vacuolation of the liver were observed only for animals given 100 ppm. The incidence and severity of hepatocellular hypertrophy and vacuolation are listed in Text Table 6. Other test material-related hepatic findings were hepatocellular necrosis for one male and two females given 100 ppm and bile stasis for one male and two females given 100 ppm. One female given 100 ppm had a hepatocellular adenoma. Hepatocellular adenomas are occasionally observed as an incidental finding in 1-year-old rats. 51 Covance 6329-212 3M T-6316.1 Text Table 6 Incidence and Severity of Hepatocellular Hypertrophy Number Examined Liver Vacuolation Minimal Slight Moderate Hypertrophy Minimal Slight Moderate Moderately Severe N-Ethyl Perfluorooctanesulfonamido (ppm) 0 100 0 100 Males Females 5 10 5 10 0 3 0 2 0 3 0 0 0 3 0 0 0 0 0 0 0 3 0 3 0 2 0 4 0 5 0 0 Unscheduled Deaths There was no test material-related effect for mortality. The only causes of death observed in three or more animals were chronic progressive nephropathy, hepatocellular adenoma, histiocytic sarcoma, mammary carcinoma, mammary fibroadenoma for females, malignant lymphoma and pituitary adenomas for both sexes. By far the most frequent cause of death was pituitary adenomas. The cause of death was undetermined for 22 males and 5 females. The incidence of the most frequently observed causes of death are listed in Text Table 7. 52 Covance 6329-212 3M T-6316.1 Text Table 7 Incidence of Test Material-Related Cause of Death Unscheduled Sacrifice Animals Examined Chronic progressive nephropathy Hepatocellular adenoma Mammary Carcinoma Mammary Fibroadenoma Histiocytic sarcoma Malignant lymphoma Pituitary adenoma Undetermined N-Ethyl Perfluorooctanesulfonamido (ppm) 0 3 30 100 0 3 30 100 Males Females 36 28 28 33 37 37 35 30 210 2 000 0 021 1 001 0 000 0 035 3 000 0 540 1 011 1 000 0 110 1 100 0 22 15 18 21 27 27 22 17 745 6 000 5 Macroscopic Findings. Diffusely dark livers were observed for four males and three females given 100 ppm. Mottled livers were more frequently observed for females given 100 ppm than for controls. The incidence of mottled livers for females was one control, two females given 3 ppm, and five females given 100 ppm. There were no other test material-related macroscopic findings. Microscopic Findings. For the unscheduled sacrifice animals, cystic degeneration and hepatocellular hypertrophy were increased for males and hepatocellular vacuolation was increased for males and females. Hepatocellular adenomas were minimally higher for treated animals compared with the controls. Thirty males and 28 females given 100 ppm that were unscheduled deaths were also examined microscopically. The incidence of test material-related microscopic findings for the unscheduled death animals is listed in Text Table 8. 53 Covance 6329-212 3M T-6316.1 Text Table 8 Incidence of Test Material-Related Microscopic Findings Unscheduled Deaths N-ethyl Perfluorooctanesulfonamido (ppm) 0 3 30 100 100 0 3 30 100 100a Males Females Animals Examined 36 28 28 33 30 37 37 35 30 28 Liver Degeneration, Cystic 33 2 6 6 00 0 0 0 Hypertrophy, Hepatocellular 0 0 0 2 2 00 0 0 0 Vacuolation, Hepatocellular 13 8 11 16 10 9 8 6 12 6 B-Adenoma, Hepatocellular 1 2 1 2 0 00 2 2 1 a Animals placed on recovery. Terminal Sacrifice Macroscopic Findings. Enlargement, mottling, and masses of the liver were more frequent for animals given 100 ppm than for the controls. There were no enlarged or diffusely dark livers for the controls at the terminal sacrifice. One male given 3 ppm and three males and one female given 100 ppm had enlarged livers. Diffusely dark livers were present for one male and four females given 100 ppm. The incidence of mottled livers was one control female, two males given 3 ppm, four males and one female given 30 ppm, and five males and three females given 100 ppm. Hepatic masses were noted at necropsy for one control male, one male and one female given 3 ppm, and three males and three females given 100 ppm. Microscopic Findings. Test material-related hepatic findings at the terminal sacrifice for animals given 100 ppm were cystic degeneration, hepatocellular hypertrophy, and vacuolation. There was only one hepatocellular carcinoma in the study and it was present for a terminal sacrifice female given 100 ppm. The incidence of test material-related microscopic findings at the terminal sacrifice is listed in Text Table 9. 54 Covance 6329-212 3M T-6316.1 Text Table 9 Incidence of Test Material-Related Microscopic Findings Terminal Sacrifice Animals Examined Liver Degeneration, Cystic Hypertrophy, Hepatocellular Vacuolation, Hepatocellular B-Adenoma, Hepatocellular M-Carcinoma, Hepatocellular N-Ethyl Perfluorooctanesulfonamido (ppm) 0 3 30 100 0 3 30 100 Males Females 14 22 22 17 13 13 15 19 3 5 12 12 00 0 5 4 8 9 13 13 3 4 00 0 0 02 01 00 05 4 6 2 12 03 24 00 01 Recovery Sacrifice Macroscopic Findings. There were no test material-related macroscopic findings at the recovery sacrifice. Microscopic Findings. The microscopic findings for the recovery sacrifice animals given 100 ppm were similar to the controls. However, 2 of the 12 females had hepatocellular adenomas. 55 Covance 6329-212 3M T-6316.1 56 Covance 6329-212 3M T-6316.1 57 Covance 6329-212 3M T-6316.1 58 Covance 6329-212 3M T-6316.1 59 Covance 6329-212 3M T-6316.1 60 Covance 6329-212 3M T-6316.1 STATISTICAL REPORT OF SURVIVAL, NONNEOPLASTIC AND NEOPLASTIC LESIONS Methods Survival. Evaluations of trend and heterogeneity of survival data were performed using the Cox-Tarone binary regression method and the National Cancer Institute (NCI) Life Table Package (Thomas, Breslow, and Gart, 1977). Week 105 was treated as the end of the study in the NCI package for both sexes. Animals that died accidentally and those that were sacrificed at scheduled intervals were censored in the analysis. Continuity corrected one-sided tail probabilities for trend and two-sided tail probabilities for group comparisons were evaluated at the 5.0% significance level as reported in Text Tables 1 and 2. Kaplan-Meier product limit survival curves are presented in Text Figures 1 and 2 for males and females, respectively. Nonneoplastic Lesions. Nonneoplastic lesions were chosen for statistical analyses if the incidence rate in at least one treated group (Groups 2 through 4) was increased or decreased by at least two occurrences over the control group (Group 1). The high recovery group (Group 6) was selected based on the incidence rate (%) if it was increased or decreased by at least two as compared to the control or high-dose group. When there was only one incidence in the high recovery and none in the other groups, that case was excluded from statistical evaluation. These were analyzed by the Cochran-Armitage test for trend and the Fisher-Irwin exact test for control versus treatment comparisons (Thakur, Berry, and Mielke, 1985). One-sided tail probabilities for trend and group comparisons were evaluated at the 5.0% significance level and are reported in Text Tables 3 and 4 for males and females, respectively. Neoplastic Lesions. Neoplastic lesions were chosen for statistical analyses if the incidence rate in at least one treated group was increased or decreased by at least two occurrences over the control group. The incidental tumors (i.e., tumors that were not assigned to be the cause of death of the animals by the study pathologist) were analyzed by logistic regression of tumor prevalence (Dinse and Lagakos, 1983). The logistic regression model included linear and quadratic terms in age (week of death), but the quadratic term was eliminated if there was a lack of convergence. The fatal and palpable (superficial) tumors were analyzed by Cox-Tarone binary regression method, as in the case of survival, using the death time or the first palpation time (as applicable) as the tumor onset time. In the case of any particular tumor type where the study pathologist assigned the tumor in question as being the cause of death of a subset of the animals and the rest of the 61 Covance 6329-212 3M T-6316.1 animals were assumed to be dead of other competing risks, IARC-type (Peto, et al., 1980) cause of death analysis was performed. Specifically, the subset of the tumors, which were assigned to be the cause of death by the examining pathologist, was analyzed by Cox-Tarone logistic regression under life table techniques. The subset, which was considered incidental by the pathologist, was analyzed by logistic regression of tumor prevalences. For the cases of tumor types where cause of death was undetermined, these were treated as incidental for statistical evaluation. The score statistics and their respective variances from the above tests were then used to compute the combined evidence as described by Gart, et al., (1986). In the case of only one tumor belonging to one of the two categories (fatal and incidental) in a test, it was combined with the other category for the purpose of statistical analyses. In addition, in cases where there was lack of convergence for the asymptotic test of the logistic regression method, the exact probability of the test was obtained by using LogXact-Turbo (1993) and combined with the probability of the Cox-Tarone test, if necessary. Common and rare tumors were evaluated at the 1.0% and 5.0% significance levels, respectively, for trend. The benign and malignant neoplastic incidences were evaluated separately, but were combined where appropriate. The criteria for combination were based on the work of McConnell et al. (1986). The incidences of multiple-organ findings such as hemangioma, hemangiosarcoma, lipoma, liposarcoma, fibroma, fibrosarcoma, leiomyoma, leiomyosarcoma, endometrial stromal polyp, and endometrial stromal sarcoma were counted by animal, not by tissue type. They were evaluated statistically if they met the selection criterion for the analysis. The statistical results for these cases may be biased because not all the animals were examined for every tissue. Pairwise one-sided group comparisons were evaluated at the 5.0% significance level. One-sided tail probabilities for trend analysis and group comparisons are shown in Text Tables 5 and 6 for males and females, respectively. Groups 5 (High-dose) and 7 (High recovery) were excluded from all the analyses except the survival graphs (Text Figures 1 and 2) due to early sacrifices at Week 8. In the nonneoplastic analyses, animals sacrificed early (Week 4) in Groups 1 through 4 (Control, Low, Mid, and Mid-High, respectively) were excluded from the denominators. In the statistical evaluations of the neoplastic lesions, animals sacrificed early (during or before Week 14) in Groups 1 through 4 were discounted from the number of animals at risk. Further, in the neoplastic analyses, in the cases where the low, mid, and mid-high recovery groups did not have a complete histopathology examination, they were excluded from statistical analyses. 62 Covance 6329-212 3M T-6316.1 In the text tables, the p-values under Group 1 (Control) are for trend analysis and the p-values under Groups 2 (Low), 3 (Mid), 4 (Mid-High), and 6 (Mid-High recovery) are for the comparison of that group versus the control group. Group 6 was also compared with Group 4, and the p-values for those comparisons are indicated in the text tables. Trend analysis includes Groups 1, 2, 3, and 4 only. Ordinal dose levels 0, 1, 2, and 3 were used in all analyses for Groups 1, 2, 3, and 4, respectively. Continuity correction was done for all asymptotic tests. The following notations are used throughout this report to designate direction and significance of effects. + = Effect in the increased direction - = Effect in the decreased direction * = Significant at p < 0.05 ** = Significant at p < 0.01 E = Exact permutation test NA = Not analyzed Results and Discussion Survival. As indicated in Text Tables 1 and 2, no significant trends or group comparisons in mortality were observed in males and females. Nonneoplastic Lesions. The liver in both sexes has been identified as the only organ that is appropriate to perform statistical analysis for nonneoplastic lesions. As indicated in Text Table 3, there were significant positive trends in the incidences of hepatocellular vacuolation and hepatocellular centrilobular hypertrophy (p = 0.00003 and 0.00000, respectively) for males. These cases were associated with significant increases in the mid-high dose group (p = 0.00009 and 0.00000, respectively), when compared with the control. However, the mid-high recovery group animals in these cases exhibited significantly decreased incidences over the mid-high dose group (p = 0.00107 and 0.00035, for both cases) implying that the effect may be transient (reversible) in nature. A significant positive trend was also observed for cystic degeneration (p = 0.00183) in association with significantly increased incidences in both the mid- and mid-high dose males over the control (p = 0.01634 and 0.00589, respectively). There was no such effect in the females for this parameter. Unless this is a sex specific effect, it can be considered to be a chance variation. Further, significantly increased incidences 63 Covance 6329-212 3M T-6316.1 were observed in the mid-high recovery males, when compared with both control and mid-high dose males for hemorrhage (p = 0.03442 and 0.00462, respectively) and the low-dose group for telangiectasis as compared to the control (p = 0.04854). However, no significant trends were found in these cases, which diminishes their importance. As indicated in Text Table 4, there were significant positive trends in the incidences of hepatocellular vacuolation, hepatocellular centrilobular hypertrophy, and bile duct hyperplasia (p = 0.02018, 0.00001, and 0.00463, respectively) for females. These cases were associated with significant increases in the mid-high-dose group (p = 0.01050, 0.00025, and 0.01750, respectively), when compared with the control. However, the mid-high recovery group animals have exhibited significantly decreased incidences over the mid-high-dose group (p = 0.02638, 0.00066, and 0.00782, respectively), which implies their transient nature. Marginally significant increases over the control were also revealed in the incidences of low- and mid-high-dose groups for chronic inflammation (p = 0.03410 and 0.02681, respectively). However, no significant trend was found in this case. Neoplastic Lesions. As indicated in Text Table 5, significant positive trends were noted only in the incidences of thyroid follicular cell adenoma and its combined adenoma and carcinoma (p = 0.0171 and 0.0155, respectively) for males. These cases were associated with significant increases in the mid-high-dose group (p = 0.0107, for both cases) over the control. However, thyroid follicular cell carcinoma did not show any significant effect. Also, the trends mentioned earlier are nonmonotonic. Follicular cell adenoma in the thyroid of the Sprague Dawley rat is a common tumor (>1% background) for which the FDA uses a 1.0% significance level for trend evaluation. Therefore, these trends are not deemed to be biologically significant. There were no other significant neoplastic findings in the males of this study. As indicated in Text Table 6, significant positive trends were observed only in the incidences of hepatocellular adenoma and combined adenoma and carcinoma (p = 0.0050 and 0.0022, respectively) for females. These cases were associated with significant increases in the mid-high-dose and mid-high recovery groups (p = 0.0120 and 0.0498, respectively for adenoma and p = 0.0069 and 0.0498, respectively, for adenoma/carcinoma), as compared with the control. However, hepatocellular carcinoma did not show a significant effect. There were significant negative trends in the incidences of mammary fibroadenoma; adenoma and fibroadenoma combined; and the combined adenoma, fibroadenoma, and carcinoma incidences (p = 0.0033, 0.0051, and 0.0122, 64 Covance 6329-212 3M T-6316.1 respectively). All these cases were associated with significantly lower incidences in the respective mid-high-dose group (p = 0.0094, 0.0113, and 0.0181, respectively). Furthermore, a marginally decreased incidence over the control was noted for the mammary combined fibroadenoma, adenoma, and carcinoma incidences (p = 0.0403). No significant trend or group comparisons were found in the incidences of mammary carcinoma. Significant negative trends were also noted in the incidences of pituitary adenoma and combined adenoma and carcinoma (p = 0.0291, for both cases). But none of the pairwise comparisons were significant in these cases. There was no significant trend or group effect in the pituitary carcinoma. There were no other significant neoplastic findings in females. References Dinse, G.E. and Lagakos, S.W., "Regression analysis of tumor prevalence data," Journal of Royal Statistical Society Series C (Applied Statistics), 32:236-248 (1983). Gart, J.J., Krewski, D., Lee, P.N., Tarone, R.E., and Wahrendorf, J., Statistical Methods in Cancer Research: The Design and Analysis of Long-Term Animal Experiments, IARC Scientific Publications, Vol. 3, No. 79, Oxford University Press, New York (1986). LogXact-Turbo, Cytel Software Corporation, Version 1.1, Cambridge, Massachusetts (1993). McConnell, E.E., Solleveld, H.A., Swenberg, J.A., and Boorman, G.A., "Guidelines for combining neoplasms for evaluation of rodent carcinogenicity studies," Journal of National Cancer Institute, 76:283-289 (1986). Peto, R., Pike, M.C., Day, N.E., Gray, R.G., Lee, P.N., Parish, S., Peto, J., Richards, S., and Wahrendorf, J., "Guidelines for simple, sensitive significance tests for carcinogenic effects in long-term animal experiments, in Long-term and Short-term Screening Assays for Carcinogens: A Critical Appraisal," Lyon: International Agency for Cancer Research (IARC), 311-426 (1980). Thakur, A.K., Berry, K.J., and Mielke, P.W., Jr., "A FORTRAN program for testing trend and homogeneity in proportions," Computer Programs in Biomedicine, 19:229-233 (1985). Thomas, D.G., Breslow, N., and Gart, J. J., "Trend and homogeneity analyses of proportions and life table data," Computers and Biomedical Research, 10:373-381 (1977). 65 Covance 6329-212 3M T-6316.1 66 Covance 6329-212 3M T-6316.1 Text Table 1 Results of Statistical Analyses of Survival Data for Male Rats Group Unadjusted Mortality Adjusted Mortality: Kaplan-Meier Estimate (Final) Standard Error 1 Control 36/70 (.514) .718 .064 2 Low 28/60 (.467) .559 .070 3 Mid 27/60 (.450) .551 .071 4 Mid-High 33/70 (.471) .657 .067 6 Mid-High Recovery 30/40 (.750) .750 .068 Groups 1 vs 2-4 Trend p (one-sided) Groups 1 vs 2 p (two-sided) Groups 1 vs 3 p (two-sided) Groups 1 vs 4 p (two-sided) Groups 1 vs 6 p (two-sided) Groups 4 vs 6 p (two-sided) Cox-Tarone Test .2438 .2381 .1736 .4952 .7216 + .2828 + Text Table 2 Results of Statistical Analyses of Survival Data for Female Rats Group Unadjusted Mortality Adjusted Mortality: Kaplan-Meier Estimate (Final) Standard Error 1 Control 37/70 (.529) .740 .062 2 Low 37/60 (.617) .740 .062 3 Mid 35/60 (.583) .700 .065 4 Mid-High 30/70 (.429) .611 .070 6 Mid-High Recovery 28/40 (.700) .700 .072 Groups 1 vs 2-4 Trend p (one-sided) Groups 1 vs 2 p (two-sided) Groups 1 vs 3 p (two-sided) Groups 1 vs 4 p (two-sided) Groups 1 vs 6 p (two-sided) Groups 4 vs 6 p (two-sided) Cox-Tarone Test .0821 .9118 .8451 .2020 .7645 .4088 + 67 Covance 6329-212 3M T-6316.1 Text Table 3 Results of Statistical Analyses of Nonneoplastic Lesions in the Males 1 2 3 Group Control Low Mid LIVER NECROSIS, HEPATOCELLULAR 1/65 3/55 4/55 p-values .33770 + .24911 + .13458 + 4 Mid-High 2/65 NA 6 Mid-High Recovery Groups 4 vs 6 4/40 .06833 + .14708 + LIVER HEMORRHAGE 3/65 3/55 p-values .24261 - NA 3/55 1/65 NA .30956 - 7/40 .03442 +* .00462 +** LIVER INFLAMMATION, CHRONIC 18/65 20/55 23/55 p-values .31850 + .20587 + .07604 + 20/65 .42364 + 9/40 .36217 - .24493 - LIVER VACUOLATION, HEPATOCELLULAR 17/65 18/55 23/55 p-values .00003 +** NA .05271 + 39/65 .00009 +** 11/40 .52672 + .00107 -** LIVER CENTRILOBULAR HYPERTROPHY, HEPATOCELLULAR 0/65 1/55 3/55 22/65 2/40 p-values .00000 +** NA .09342 + .00000 +** .14286 + .00035 -** LIVER HYPERPLASIA, BILE DUCT 9/65 9/55 8/55 p-values .37867 + NA NA 11/65 .40426 + 5/40 .54603 - .37555 - LIVER DEGENERATION, CYSTIC 6/65 8/55 14/55 p-values .00183 +** .26759 + .01634 +* 18/65 .00589 +** 7/40 .17208 + .17015 - LIVER FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC 8/65 12/55 11/55 6/65 p-values .32152 - .12579 + .18421 + .38915 - 2/40 .18700 - .34905 - LIVER TELANGIECTASIS 2/65 7/55 3/55 0/65 0/40 p-values .12470 - .04854 +* NA .24806 - .38095 - NA LIVER CYST 1/65 0/55 0/55 2/65 p-values .30780 + NA NA NA 0/40 NA .38095 - LIVER LIPIDOSIS, TENSION 0/65 2/55 1/55 2/65 0/40 p-values .21735 + .20798 + NA .24806 + NA .38095- LIVER FIBROSIS 0/65 0/55 1/55 0/65 p-values NA NA NA NA 2/40 .14286 + .14286 + 68 Covance 6329-212 3M T-6316.1 Text Table 4 Results of Statistical Analyses of Nonneoplastic Lesions in the Females 1 2 3 Group Control Low Mid LIVER NECROSIS, HEPATOCELLULAR 5/65 4/55 4/55 p-values .41816 + NA NA LIVER HEMORRHAGE 4/65 2/55 4/55 p-values .10451 - .42223 - NA 4 Mid-High 6/65 NA 6 Mid-High Recovery Groups 4 vs 6 3/40 .65095 - .53010 - 0/65 3/40 .05960 - NA .05270 + LIVER HEMATOPOIESIS, EXTRAMEDULLARY 1/65 1/55 0/55 3/65 1/40 p-values .21735 + NA NA .30956 + NA .50714 - LIVER INFLAMMATION, CHRONIC 9/65 16/55 10/55 p-values .06223 + .03410 +* NA 19/65 .02681 +* 8/40 .28521 + .20687 - LIVER VACUOLATION, HEPATOCELLULAR 13/65 14/55 8/55 p-values .02018 +* NA .29527 - 26/65 .01050 +* 8/40 .59441 .02638 -* LIVER CENTRILOBULAR HYPERTROPHY, HEPATOCELLULAR 1/65 0/55 0/55 14/65 0/40 p-values .00001 +** NA NA .00025 +** .61905 - .00066 -** LIVER HYPERPLASIA, BILE DUCT 4/65 3/55 6/55 p-values .00463 +** NA .27110 + 13/65 .01750 +* 1/40 .3660 - .00782 -** LIVER DEGENERATION, CYSTIC 0/65 2/55 0/55 2/65 1/40 p-values .25667 + .20798 + NA .24806 + NA NA LIVER STASIS, BILE 0/65 0/55 0/55 2/65 0/40 p-values .06250 + NA NA .24806 + NA .38095 - LIVER FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC 0/65 2/55 2/55 1/65 0/40 p-values .34805 + .20798 + .20798 + NA NA NA LIVER FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC 3/65 7/55 2/55 7/65 2/40 p-values .26768 + .10200 + NA .16203 + NA .25853 - 69 Covance 6329-212 3M T-6316.1 Text Table 4 (Continued) Results of Statistical Analyses of Nonneoplastic Lesions in the Females 1 2 Group Control Low LIVER TELANGIECTASIS 3/65 6/55 p-values .45263 - .16964 + LIVER LIPIDOSIS, TENSION 0/65 1/55 p-values NA NA 3 Mid 1/55 .37488 - 0/55 NA 4 Mid-High 4/65 NA 0/65 NA 6 Mid-High Recovery 5/40 .13634 + 2/40 .14286 + Groups 4 vs 6 .21870 + .14286 + LIVER FIBROSIS 0/65 3/55 1/55 0/65 1/40 p-values .41376 - .09342 + NA NA NA NA 70 Covance 6329-212 3M T-6316.1 Text Table 5 Results of Statistical Analyses of Neoplastic Lesions - Male Rats 1 2 Group Control Low Liver - Hepatocellular, Adenoma Fatal Incidence 0 2 Incidental Incidence 2 2 Total Incidence Rate 2/55 4/50 3 4 6 Mid Mid-High Mid-High Recovery Groups 4 vs 6 1 1 0 1 4 0 2/50 5/60 0/40 One-sided p-values .2514 + .3324 + NA .1883 + .3149 - .0854 - Pancreas - Islet Cell, Adenoma Fatal Incidence 0 0 0 0 0 Incidental Incidence 4 7 2 6 2 Total Incidence Rate 4/55 7/50 2/50 6/60 2/40 One-sided p-values .4817 - .2869 + .2575 - .3040 + .4753 - .3161 - Pancreas - Islet Cell, Carcinoma Fatal Incidence 0 0 0 0 0 Incidental Incidence 0 0 1 0 0 Total Incidence Rate 0/55 0/50 1/50 0/60 0/40 Note: Incidences across groups do not meet the selection criterion. 71 Text Table 5 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Male Rats 1 2 Group Control Low Pancreas - Islet Cell, Adenoma/Carcinoma Fatal Incidence 0 0 Incidental Incidence 4 7 Total Incidence Rate 4/55 7/50 3 4 6 Mid Mid-High Mid-High Recovery 0 0 0 3 6 2 3/50 6/60 2/40 One-sided p-values .4678 + .2869 + .4072 - .3040 + .4753 - Adrenal Medulla - Pheochromocytoma, Benign Fatal Incidence 0 0 0 0 0 Incidental Incidence 9 6 4 5 1 Total Incidence Rate 9/55 6/29 4/31 5/60 1/31 One-sided p-values NA NA NA .2227 - NA Testis - Interstitial Cell Tumor, Benign Fatal Incidence 0 0 0 0 0 Incidental Incidence 3 1 3 1 0 Total Incidence Rate 3/55 1/50 3/50 1/60 0/40 One-sided p-values .2566 - .2620 - NA .1646 - .1779 - Covance 6329-212 3M T-6316.1 Groups 4 vs 6 .3161 - NA NA 72 Covance 6329-212 3M T-6316.1 Text Table 5 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Male Rats 1 Group Control Thyroid - C-Cell, Adenoma Fatal Incidence 0 Incidental Incidence 10 Total Incidence Rate 10/55 2 Low 0 10 10/50 3 Mid 0 10 10/50 4 6 Mid-High Mid-High Recovery Groups 4 vs 6 0 0 12 5 12/60 5/40 One-sided p-values .3344 + NA NA .3320 + .3100 - .1843 - Thyroid - C-Cell, Carcinoma Fatal Incidence 0 0 0 0 0 Incidental Incidence 0 0 1 0 1 Total Incidence Rate 0/55 0/50 1/50 0/60 1/40 Note: Incidences across groups do not meet the selection criterion. Thyroid - C-Cell, Adenoma/Carcinoma Fatal Incidence 0 0 0 0 0 Incidental Incidence 10 10 11 12 6 Total Incidence Rate 10/55 10/50 11/50 12/60 6/40 One-sided p-values .3124 + NA NA .3320 + .3100 - .1843 - 73 Covance 6329-212 3M T-6316.1 Text Table 5 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Male Rats 1 2 Group Control Low Thyroid - Follicular Cell, Adenoma Fatal Incidence 0 0 Incidental Incidence 0 3 3 4 6 Mid Mid-High Mid-High Recovery Groups 4 vs 6 0 0 0 1 6 1 One-sided p-values .0171 +* .1662 + NA .0107 +* NA .1204 - Thyroid - Follicular Cell, Carcinoma Fatal Incidence 0 0 0 0 0 Incidental Incidence 0 0 1 0 1 Total Incidence Rate 0/55 0/50 1/50 0/60 1/40 Note: Incidences across groups do not meet the selection criterion. Thyroid - Follicular Cell, Adenoma/Carcinoma Fatal Incidence 0 0 0 0 0 Incidental Incidence 0 3 2 6 2 Total Incidence Rate 0/55 3/50 2/50 6/60 2/40 One-sided p-values .0155 +* .1662 + .3667 +(E) .0107 +* .1713 + .2303 - 74 Covance 6329-212 3M T-6316.1 Text Table 5 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Male Rats Group Pituitary - Adenoma Fatal Incidence Incidental Incidence Total Incidence Rate 1 Control 23 23 46/55 2 Low 15 13 28/33 3 Mid 17 19 36/38 4 6 Mid-High Mid-High Recovery Groups 4 vs 6 22 26 48/60 20 11 31/36 One-sided p-values NA NA NA .4108 - .1616 + .1037 + Skin - Papilloma, Squamous Cell Total Incidence Rate 0/55 3/35 0/33 2/60 3/32 One-sided p-values NA NA NA .2509 + NA NA Multiple Organs - Fibroma Total Incidence Rate 2/55 1/35 1/33 0/60 0/32 One-sided p-values NA NA NA .1957 -(E) NA NA Multiple Organs - Fibrosarcoma Total Incidence Rate 1/55 0/29 0/28 0/60 0/30 Note: Incidences across groups do not meet the selection criterion. Multiple Organs - Fibroma/Fibrosarcoma Total Incidence Rate 3/55 1/35 1/33 0/60 0/32 One-sided p-values NA NA NA .0946 - NA 75 Covance 6329-212 3M T-6316.1 Text Table 6 Results of Statistical Analyses of Neoplastic Lesions - Female Rats 1 2 Group Control Low Liver - Hepatocellular, Adenoma Fatal Incidence 0 0 Incidental Incidence 0 1 Total Incidence Rate 0/55 1/50 3 4 6 Mid Mid-High Mid-High Recovery Groups 4 vs 6 1 0 0 2 6 3 3/50 6/60 3/40 One-sided p-values .0050 +** NA .0796 + .0120 +* .0498 +* .4879 - Liver - Hepatocellular, Carcinoma Fatal Incidence 0 0 0 0 0 Incidental Incidence 0 0 0 1 0 Total Incidence Rate 0/55 0/50 0/50 1/60 0/40 Note: Incidences across groups do not meet the selection criterion. Liver - Hepatocellular, Adenoma/Carcinoma Fatal Incidence 0 0 1 0 0 Incidental Incidence 0 1 2 7 3 Total Incidence Rate 0/55 1/50 3/50 7/60 3/40 One-sided p-values .0022 +** NA .0796 + .0069 +** .0498 +* .3805 - 76 Covance 6329-212 3M T-6316.1 Text Table 6 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Female Rats 1 2 Group Control Low Pancreas - Islet Cell, Adenoma Fatal Incidence 0 0 Incidental Incidence 1 4 Total Incidence Rate 1/55 4/50 3 4 6 Mid Mid-High Mid-High Recovery Groups 4 vs 6 0 0 0 1 1 1 1/50 1/60 1/40 One-sided p-values .2252 -(E) .1609 +(E) NA NA NA NA Adrenal Medulla - Pheochromocytoma, Benign Fatal Incidence 0 0 Incidental Incidence 0 2 Total Incidence Rate 0/55 2/40 1/39 2/60 1/30 One-sided p-values NA NA NA .3448 +(E) NA NA Thymus - Thymic, Carcinoma Fatal Incidence 0 0 0 1 0 Incidental Incidence 0 0 0 1 0 Total Incidence Rate 0/55 0/36 0/35 2/59 0/25 One-sided p-values NA NA NA .1029 + NA NA 77 Covance 6329-212 3M T-6316.1 Text Table 6 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Female Rats 1 Group Control Thyroid - C-Cell, Adenoma Fatal Incidence 0 Incidental Incidence 8 Total Incidence Rate 8/55 2 Low 0 13 13/50 3 4 6 Mid Mid-High Mid-High Recovery Groups 4 vs 6 0 0 0 8 11 8 8/50 11/60 8/40 One-sided p-values .4920 + .1323 + NA .3258 + NA .4676 + Thyroid - C-Cell, Carcinoma Fatal Incidence 1 0 0 0 0 Incidental Incidence 0 0 0 0 0 Total Incidence Rate 1/55 0/50 0/50 0/60 0/40 Note: Incidences across groups do not meet the selection criterion. Thyroid - C-Cell, Adenoma/Carcinoma Fatal Incidence 1 0 0 0 0 Incidental Incidence 8 13 8 11 8 Total Incidence Rate 9/55 13/50 8/50 11/60 8/40 One-sided p-values .4866 - .1990 + NA .4095 + NA .4676 + 78 Covance 6329-212 3M T-6316.1 Text Table 6 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Female Rats 1 2 Group Control Low Thyroid - Follicular Cell, Adenoma Fatal Incidence 0 0 Incidental Incidence 1 0 Total Incidence Rate 1/55 0/50 3 4 6 Mid Mid-High Mid-High Recovery Groups 4 vs 6 0 0 0 2 4 1 2/50 4/60 1/40 One-sided p-values .0617 + NA NA .1139 + NA .2909 - Pituitary - Adenoma Fatal Incidence Incidental Incidence Total Incidence Rate 26 22 48/55 28 18 46/48 23 25 48/49 17 28 45/59 16 18 34/38 One-sided p-values .0291 -* .3193 + NA .0962 - .3276 - .1149 + Pituitary - Carcinoma Fatal Incidence 0 0 0 0 1 Incidental Incidence 0 0 0 0 0 Total Incidence Rate 0/55 0/48 0/49 0/59 1/38 Note: Incidences across groups doe not meet the selection criterion 79 Covance 6329-212 3M T-6316.1 Text Table 6 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Female Rats 1 Group Control Pituitary - Adenoma/Carcinoma Fatal Incidence 26 Incidental Incidence 22 Total Incidence Rate 48/55 2 Low 28 18 46/48 3 Mid 23 25 48/49 4 6 Mid-High Mid-High Recovery Groups 4 vs 6 17 28 45/59 17 18 35/38 One-sided p-values .0291 -* .3193 + NA .0962 - .4362 - .0644 + Mammary - Adenoma Total Incidence Rate 6/54 4/50 6/50 3/60 4/40 One-sided p-values .1522 - .3187 - NA .1492 - .4486 - .3332 + Mammary - Fibroadenoma Total Incidence Rate 23/54 21/50 16/50 13/60 12/40 One-sided p-values .0033 -** .4531 - .0727 - .0094 -** .0525 - .3506 + Mammary - Adenoma/Fibroadenoma Total Incidence Rate 26/54 24/50 21/50 16/60 15/40 One-sided p-values .0051 -** .3923 - .1200 - .0113 -* .0801 - .2876 + 80 Covance 6329-212 3M T-6316.1 Text Table 6 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Female Rats 1 2 3 4 6 Group Control Low Mid Mid-High Mid-High Recovery Groups 4 vs 6 Mammary - Carcinoma Total Incidence Rate 9/54 9/50 15/50 11/60 6/40 One-sided p-values .3277 + NA .1399 + .4323 + .4108 - .3616 - Mammary - Adenoma/Fibroadenoma/Carcinoma Total Incidence Rate 32/54 29/50 28/50 23/60 18/40 One-sided p-values .0122 -* .4055 - .2364 - .0181 -* .0403 -* .4781 + Multiple Organs - Leiomyoma Total Incidence Rate 3/55 0/41 0/39 1/60 1/32 One-sided p-values NA NA NA .1710 -(E) NA NA 81 Covance 6329-212 3M T-6316.1 TEXT FIGURE 1 KAPLAN-MEIER PRODUCT LIMIT ESTIMATE CURVES OF SURVIVAL: MALE RATS 0 15 30 45 60 75 90 105 + + + + + + + + ------------------------------------------------------------------------ 1.0+**********22222222222222222222222222 + 6*************************3 2222222 + 45 111111***********0*2222 + 5555555555555555555555 1*3*33 22 + 555555 1 003 2 .9+ 55555***0*33* P+ ****1*2 R+ 50***2 O+ 555**22 P+ 55*** O .8+ 0***5 R+ 0*0**5 T+ 33*** I+ ***5 O+ *** N .7+ ****3 + **0* S+ 2***2222 U+ 55***1* R+ 5 0*3* V .6+ 55 03**2 I+ 5 00**2 V+ 5 0**22 I+ 5 0*** N+ 555 0 3*2 G .5+ 55 0 ***22 + 55*0 1* 2 + 50 * 2 + 500 *1** + 5 00 33 .4+ 555* 33 + *0 3 + 500 3 + 55* 33 + * .3+ ** + **0 + 5 + 55 + .2+ 0 = Group 1 (Control) + 1 = Group 2 (Low) + 2 = Group 3 (Mid) + 3 = Group 4 (Mid-High) + 4 = Group 5 (High) .1+ 5 = Group 6 (Mid-High Recovery) + 6 = Group 7 (High Recovery) + * = More Than One Data Point + + .0+ ------------------------------------------------------------------------ + + + + + + + + 0 15 30 45 60 75 90 105 TIME (WEEKS) 82 Covance 6329-212 3M T-6316.1 TEXT FIGURE 2 KAPLAN-MEIER PRODUCT LIMIT ESTIMATE CURVES OF SURVIVAL: FEMALE RATS 0 15 30 45 60 75 90 105 + + + + + + + + ------------------------------------------------------------------------ 1.0+*************************************55555 + 3333333333333333********* 55 + **** + 22***1 + 0***** .9+ 00*** P+ ****0 R+ 123*0 O+ 1**** P+ 22*0 O .8+ 2** R+ 25**3 T+ 22*53 I+ 2**** O+ 21* **3 N .7+ 2 * 5*33 + 22***25 3 S+ 0*** 3 U+ 00***3 R+ 0***33 V .6+ 2** 3333 I+ **0 33 V+ 2*0 333 I+ **5 3 N+ *** 3 G .5+ 00** 3 + 01** 333 + 005* 3 + 0***2 333 + **** 3 .4+ 05* 3 + 05*2 33 + 00*22 + 0* 222 + **5 2 .3+ 0***** + 11* + ** + + .2+ 0 = Group 1 (Control) + 1 = Group 2 (Low) + 2 = Group 3 (Mid) + 3 = Group 4 (Mid-High) + 4 = Group 5 (High) .1+ 5 = Group 6 (Mid-High Recovery) + 6 = Group 7 (High Recovery) + * = More Than One Data Point + + .0+ ------------------------------------------------------------------------ + + + + + + + + 0 15 30 45 60 75 90 105 TIME (WEEKS) 83 Covance 6329-212 3M T-6316.1 COMMENTS ON THE DATA Various models of calculators, computers, and computer programs were used to analyze data in this study. Because different models round off or truncate numbers differently, values in some tables (e.g., means, standard deviations, or individual values) may differ slightly from those in other tables, from individually calculated data, or from statistical analysis data. Neither the integrity nor the interpretation of the data was affected by these differences. The units for the dose levels in the inlife summary tables are ppm. The number of animals listed in the heading of the summary tables for clinical and mass observations reflects the number of animals assigned to each group at the start of the study. The summary tables for clinical observations indicates the number of animals for which a condition was observed without regard to the reversibility, number of incidences/animal, or the length of time the condition persisted. Only observations for each animal other than normal are indicated in the summary clinical observations tables. Each animal with observations recorded as "Normal" throughout the study has the comment "No remarkable observations" indicated in the individual clinical observations tables. The specific details for comments in the individual clinical observations tables that are indicated with a "C" can be found at the end of each group for each sex. The values for summary and individual test material consumption are reported with more significant figures than is appropriate for the data due to limitations of the data collection and reporting software. Food consumption values are reported in whole numbers; however, the data collection system carries food consumption values to one place to the right of the decimal for food efficiency and test material consumption calculations. The differences in values generated do not influence the interpretation of the calculation. The comment "SPILLED" in individual food consumption data tables indicates that food consumption was not recorded due to spillage during the interval. The comment "CNTMD" in individual food consumption data tables indicates that food consumption was not recorded due to contamination of the diet with urine or feces in the food container. 84 Covance 6329-212 3M T-6316.1 COMMENTS ON THE DATA (Continued) The comment "NOT TAKEN" in individual food consumption data tables indicates that the animal died before the end of the food consumption interval. Differences in the population size (N) in the summary tables for clinical and anatomic pathology are explained in the individual data tables or the codes sheets or in the Protocol Deviations section. Results of clinical pathology samples collected for animals sacrificed at unscheduled intervals appear in the individual clinical pathology tables for the next scheduled interval. These results are not in the summary tables and are not included in the statistical analyses. Statistical analyses were not done when there were two or fewer values for a parameter. The calculation for individual test material consumption is: Test material consumption = Diet ConcentrationxFood Consumption Food Consumption Intervalx[First Body Weight of Interval+ (Daily Body Weight GainxDay Factor)] where: Daily Body Weight Gain = Last Body Weight of Interval- First Body Weight of Interval Day of Last Body Weight of Interval- Day of First Body Weight of Interval and: Day Factor = (Food Consumption Start Day- Day of First Body Weight of Interval) + Food Consumption Interval 2 and: Food Consumption Interval = Food Consumption End Day- Food Consumption Start Day 85 CODES, ABBREVIATIONS, AND UNITS Covance 6329-212 3M T-6316.1 General Codes and Abbreviations Codes for Clinical Pathology Abbreviations and Units for Clinical Hematology Abbreviations and Units for Clinical Chemistry Abbreviations and Units for Clinical Urinalysis Abbreviations and Units for Urine Chemistry Codes for Anatomic Pathology Note: The following lists of codes, abbreviations, and units are used by Covance. Some, but not necessarily all, of this information may be needed for this report. 86 Covance 6329-212 3M T-6316.1 General Codes and Abbreviations WK N Mean; MEAN CAM SD; S.D.; STAND DEV; STANDARD DEV; sd * -; NA P C UNSCHED DISPATCH Week Number of measurements in a group Arithmetic mean Covariate-adjusted mean Standard deviation Group mean is significantly different from the mean of the control group (Group 1) at p < 0.05 No value; not applicable; not present Present Comment found at the end of each group for each sex Unscheduled Observations transferred from the inlife module of the data collection system to the necropsy module for reference during necropsy. Observations are duplicates of the last inlife observations. Animal Death Codes 1 2 3 4 6 T D M U Interim sacrifice, Week 4 Interim sacrifice, Week 8 Interim sacrifice, Week 14 Interim sacrifice, Week 52 Interim sacrifice, Week 8 Terminal sacrifice Found dead Moribund First postrecovery sacrifice 87 NS QS/QNS NR FS SC SH H SL L SI I NF U DT/DOT DB TJ TE RE EE SE PC PD PI PL PA CO HB PLASMO NO AGG FR UTD NO COAG Covance 6329-212 3M T-6316.1 Codes for Clinical Pathology GENERAL CODES No sample Quantity not sufficient No repeat (sample volume not sufficient for repeat analysis) Fibrin strands Sample clotted Slightly hemolyzed Hemolyzed Slightly lipemic Lipemic Slightly icteric Icteric Animal not fasted Unscheduled/moribund bleed Animal died on test Died during bleeding Technician judgment to repeat test Technical error (instrument or technician error that results in unacceptable data, e.g., unacceptable instrument output, sample spilled, entry of invalid data) Recording error (recorded incorrect data, e.g., wrong number, spelling error, incorrect date) Entry error (incorrect keyboard entry) Sampling error Platelets clumped Platelets decreased Platelets increased Platelets large Platelets appear adequate Color interferes with test Heinz bodies observed Plasmodium No aggregation Fractious Unable to determine No coagulation 88 Covance 6329-212 3M T-6316.1 Codes for Clinical Pathology (Continued) RESULTS NOT INCLUDED IN STATISTICAL ANALYSES Hemolyzed clinical chemistry or coagulation samples Samples from animals at unscheduled intervals Prothrombin times (PT) greater than 50 seconds Activated partial thromboplastin times (PTT) greater than 110 seconds Bleed times (BLETIME) greater than 30 minutes CODES FOR BLOOD CELL MORPHOLOGY The following scale was used to measure the degree of anisocytosis (ANISO), poikilocytosis (POIK), polychromasia (POLY), hypochromasia (HYPO), or basophilic stippling (BASTIP) or the presence of Howell-Jolly bodies (HJBODY), toxic neutrophils (TOXNEUT), or atypical lymphocytes (ATYPLYM). Scale 1 2 3 4 Degree Normal for the species Slight Moderate Marked Not applicable Presence Not present Rare Few Moderate Many URINE APPEARANCE A Pale B Straw C Yellow D Dark yellow Color E Amber F Brown G Red H Green I Black P Blue/green Q Blue R Orange Clarity J Clear K Hazy L Cloudy Miscellaneous M Debris O Feces 89 Covance 6329-212 3M T-6316.1 Codes for Clinical Pathology (Continued) URINE CHEMISTRY MULTISTIX STRIP Urine Glucose + ++ +++ ++++ +++++ Negative 100 mg/dL 250 mg/dL 500 mg/dL 1,000 mg/dL >2,000 mg/dL Urine Ketone + ++ +++ ++++ +++++ Negative 5 mg/dL 15 mg/dL 40 mg/dL 80 mg/dL 160 mg/dL Urine Blood - Negative + Small ++ Moderate +++ Large Urine Urobilinogen + ++ +++ ++++ 0.2 mg/dL 1 mg/dL 2 mg/dL 4 mg/dL 8 mg/dL (1 mg = approximately 1 Ehrlich unit) Urine Bilirubin - Negative + Small ++ Moderate +++ Large URINE SEDIMENT Cells, Crystals, Casts, and Comments A Amorphous urates B Amorphous phosphates C Uric acid D Triple phosphates E Calcium oxalate F Calcium carbonate G Granular casts H Hyaline casts I Cellular casts J Waxy casts K Unknown crystal P Mucous threads Q Sperm R Fecal contamination S Pinworm ova found T Pinworm larvae found U Parasite ova found 0 Not present 1 1 - 5 per field 2 6 - 10 per field 3 11 - 20 per field 4 >20 per field Bacteria 0 Not present 1 Few 2 Moderate 3 Many 90 Covance 6329-212 3M T-6316.1 Abbreviations and Units for Clinical Hematology Test Red blood cell count Hemoglobin Hematocrit Mean corpuscular volume Mean corpuscular hemoglobin Mean corpuscular hemoglobin concentration Platelet count Mean platelet volume Reticulocyte count Absolute reticulocyte count Heinz body count Erythrocyte sedimentation rate Prothrombin time Activated partial thromboplastin time Thrombin time Activated coagulation time Fibrinogen Fibrin/fibrinogen degradation products Platelet aggregation Collagen Adenosine diphosphate Alpha 2-antiplasmin Bleeding time Methemoglobin Plasma hemoglobin Myeloid/erythroid ratio Estimated myeloid/erythroid ratio White blood cell count Differential blood cell count Segmented neutrophil count Lymphocyte count Monocyte count Eosinophil count Basophil count Anisocytosis Polychromasia Poikilocytosis Toxic neutrophils Abbreviation (Units) RBC (E6/UL or X106/.L) HGB (G/DL) HCT (%) MCV (FL) MCH (PG) MCHC (%) PLT (E3/UL or X103/.L) MPV (FL) RETIC (%) RETIC (E3/UL or X103/.L) HEINZ (%) ESR (MM/HR) PT (SEC) PTT (SEC) TT (SEC) ACT (SEC) FBR (MG/DL) FDP (UG/ML) PAGG/COL (%) PAGG/ADP (%) ANTIPLAS (%) BLE TIME (SEC) METHGB (%) PLA HGB (MG/DL) M/E RATIO EST M/E RATIO WBC (E3/UL or X103/.L) N-SEG (E3/UL or X103/.L) and % LYMPH (E3/UL or X103/.L) and % MONO (E3/UL or X103/.L) and % EOSIN (E3/UL or X103/.L) and % BASO (E3/UL or X103/.L) and % ANISO (-,1,2,3) POLY (-,1,2,3) POIK (-,1,2,3) TOXNEUT (-,1,2,3,4) 91 Covance 6329-212 3M T-6316.1 Abbreviations and Units for Clinical Chemistry Test Glucose Urea nitrogen Creatinine Total protein Albumin Globulin Total bilirubin Cholesterol Triglyceride Aspartate aminotransferase Alanine aminotransferase Gamma glutamyltransferase Palmitoyl CoA oxidase Calcium Inorganic phosphorus Sodium Potassium Chloride Abbreviation (Units) GLU (MG/DL) UN (MG/DL) CREAT (MG/DL) T PRO (G/DL) ALB (G/DL) GLOB (G/DL) T BILI (MG/DL) CHOL (MG/DL) TRIG (MG/DL) AST/SGOT (IU/L) ALT/SGPT (IU/L) GGT (IU/L) PCOAO (IU/G) CA (MG/DL) I PHOS (MG/DL) NA (MMOL/L) K (MMOL/L) CL (MMOL/L) 92 Covance 6329-212 3M T-6316.1 Abbreviations and Units for Clinical Urinalysis Test Urine volume Specific gravity Urine chemistry Multistix strip Urine pH Urine protein Urine glucose Urine ketones Urine bilirubin Urine blood Urine urobilinogen Microscopic examination of urine sediment Red blood cells per high-power field White blood cells per high-power field Epithelial cells per high-power field Bacteria per high-power field Casts per low-power field Crystals per low-power field Urine appearance Comments Abbreviation (Units) U VOL (ML) SP GR U PH U PRO (MG/DL) U GLU U KET U BILI U BLOOD UROBILI RBC (PER HPF) WBC (PER HPF) EPITH (PER HPF) BACT (PER HPF) CASTS (PER LPF) CRYSTALS (PER LPF1 or PER LPF2) URINE APP1 or URINE APP2 COMMENTS 93 Covance 6329-212 3M T-6316.1 Abbreviations and Units for Urine Chemistry Test Urine sodium Urine potassium Sodium excretion Potassium excretion Abbreviation (Units) U NA (MMOL/L) U K (MMOL/L) NA EXC (MMOL) K EXC (MMOL) 94 Code EX NOT TAKEN MISSING UNSUITABLE AUTOLYTIC EXCLUDE Covance 6329-212 3M T-6316.1 Codes for Anatomic Pathology Definition MACROSCOPIC CODES Indicates that organ weight is excluded from calculations Organ weight not taken; explanation given in necropsy notes Organ missing or lost Organ technically unsuitable for weighing Organ autolyzed and could not be weighed Weight was taken, but was excluded from all calculations MICROSCOPIC CODES Codes Prefacing Neoplastic Findings B- Primary, benign neoplasm M- Primary, malignant neoplasm N- Metastatic neoplasm I- Locally invasive neoplasm X- Other neoplasm Distribution of Findings Focal Diffuse Multifocal Grades for Severity or Amount 1 Minimal - the least amount of change that can be observed with the light microscope 2 Slight - less than average amount of change, but readily discernible as abnormal 3 Moderate - the average amount of change that is expected for a lesion 4 Moderately severe (marked) - a marked amount of change with possible loss of function of the affected cells or organs 5 Severe - a great amount of change with probable loss of function of the affected cell or organs and frequently involves large areas of the organ 95 Codes for Anatomic Pathology (Continued) Other Microscopic Codes TL Total P Finding present - Finding not present MN Mean Covance 6329-212 3M T-6316.1 TISSUE ABBREVIATIONS Abbreviation LN GL STOMACH, GL STOMACH, NONGL SALIV GL, MANDIB LN, ANT MES/PANC AUDITORY SEB GL LACRIMAL GLAND, EX HEMATO NEOPLASIA LACRIMAL GL, INT CAVITY, ABDOM SALIV GL,PAROTID LN, TRACHEOBRON Definition Lymph node Gland Glandular stomach Nonglandular stomach Mandibular salivary gland Anterior mesenteric/pancreatic lymph node Auditory sebaceous gland Exorbital lacrimal gland Hematopoietic neoplasia Internal lacrimal gland Abdominal cavity Parotid salivary gland Tracheobronchial lymph node 96 Figure 1 Adjusted Survival Data (%) - Males Covance 6329-212 3M T-6316.1 100 104-W EEK DIETARY CARCINOG ENICITY STUDY W ITH NARRO W RANG E (98.1% ) N-ETHYL PERFLUROOCTANESULFONAMIDO ETHANOL IN RATS 90 80 70 60 50 Group 1 - 0 ppm 40 Group 2 - 3 ppm Group 3 - 30 ppm 30 Group 4 - 100 ppm Group 5 - 300 ppm 20 Group 6 - 100 ppm Group 7 - 300 ppm 10 0 0 4 8 12 16 20 24 28 32 36 40 44 48 52 56 60 64 68 72 76 80 84 88 92 96 100 104 W eek (%) 97 Figure 2 Adjusted Survival Data (%) - Females Covance 6329-212 3M T-6316.1 100 104-W EEK DIETARY CARCINOGENICITY STUDY W ITH NARROW RANGE (98.1%) N-ETHYL PERFLUROOCTANESULFONAMIDO ETHANOL IN RATS 90 80 70 60 50 Group 1 - 0 ppm 40 Group 2 - 3 ppm Group 3 - 30 ppm 30 Group 4 - 100 ppm Group 5 - 300 ppm 20 Group 6 - 100 ppm Group 7 - 300 ppm 10 0 0 4 8 12 16 20 24 28 32 36 40 44 48 52 56 60 64 68 72 76 80 84 88 92 96 100 104 W eek (%) 98 Figure 3 Mean Body Weight Data (g) - Males Covance 6329-212 3M T-6316.1 900 104-W EEK DIETARY CARCINOGENICITY STUDY W ITH NARROW RANGE (98.1%) N-ETHYL PERFLUROOCTANESULFONAMIDO ETHANOL IN RATS 800 700 600 Group 1 - 0 ppm Group 2 - 3 ppm Group 3 - 30 ppm 500 Group 4 - 100 ppm Group 5 - 300 ppm Group 6 - 100 ppm 400 Group 7 - 300 ppm 300 200 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 100 105 Recovery period for Group 6 started W eek 53. Week (g) 99 Figure 4 Mean Body Weight Data (g) - Females Covance 6329-212 3M T-6316.1 104-W EEK DIETARY CARCINOGENICITY STUDY W ITH NARROW RANGE (98.1%) N-ETHYL PERFLUROOCTANESULFONAMIDO ETHANOL IN RATS 550 500 450 400 350 Group 1 - 0 ppm 300 Group 2 - 3 ppm Group 3 - 30 ppm Group 4 - 100 ppm 250 Group 5 - 300 ppm Group 6 - 100 ppm 200 Group 7 - 300 ppm 150 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 100 105 Recovery period for Group 6 started W eek 53. W eek (g) 100 Figure 5 Mean Food Consumption Data (g) - Males Covance 6329-212 3M T-6316.1 104-W EEK DIETARY CARCINOGENICITY STUDY W ITH NARROW RANGE (98.1%) N-ETHYL PERFLUROOCTANESULFONAMIDO ETHANOL IN RATS 215 205 195 185 175 Group 1 - 0 ppm 165 Group 2 - 3 ppm Group 3 - 30 ppm Group 4 - 100 ppm 155 Group 5 - 300 ppm Group 6 - 100 ppm 145 Group 7 - 300 ppm 135 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 100 105 Recovery period for Group 6 started W eek 53. W eek (g) 101 Figure 6 Mean Food Consumption Data (g) - Females Covance 6329-212 3M T-6316.1 170 104-W EEK DIETARY CARCINOGENICITY STUDY W ITH NARROW RANGE (98.1%) N-ETHYL PERFLUROOCTANESULFONAMIDO ETHANOL IN RATS 160 150 140 130 Group 1 - 0 ppm 120 Group 2 - 3 ppm Group 3 - 30 ppm 110 Group 4 - 100 ppm Group 5 - 300 ppm Group 6 - 100 ppm 100 Group 7 - 300 ppm 90 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 100 105 Recovery period for Group 6 started W eek 53. Week (g) 102 Covance 6329-212 3M T-6316.1 Table 1 Results of Homogeneity Analyses (ppm) Mixed 9/11/97 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Sample Location Replicate 3 T-6316 (ppm) 30 100 300 Top 1 6.28 38.0 95.3 322 2 6.35 32.2 90.9 277 3 Mean 6.24 33.7 93.5 296 6.29 (210)a 34.6 (115) 93.2 (93.2) 298 (99.3) Middle 1 2 3 Mean 5.17 5.65 5.89 5.57 (186) 33.9 34.1 34.9 34.3 (114) 102 143 96.8 114 (114) 286 271 266 274 (91.3) Bottom 1 2 3 Mean 6.61 5.24 5.37 5.74 (191) 32.9 35.9 32.7 33.8 (113) 109 108 99.4 105 (105) 325 334 278 312 (104) a Each value in parentheses is the percent of theoretical 103 Covance 6329-212 3M T-6316.1 Table 1 (Continued) Results of Homogeneity Analyses (ppm) Mixed 9/29/97 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS T-6316 (ppm) Sample Location Replicate 3 Top 1 3.98 2 4.26 3 Mean 30.0 12.7 (423)a Middle 1 2 3 Mean 4.00 4.14 3.87 4.00 (133) Bottom 1 2 3 Mean 4.32 3.98 4.20 4.17 (139) a Each value in parentheses is the percent of theoretical. 104 Covance 6329-212 3M T-6316.1 Table 1 (Continued) Results of Homogeneity Analyses (ppm) Mixed 10/15/97 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS T-6316 (ppm) Sample Location Replicate 3 Top 1 3.18 2 2.99 3 Mean 3.23 3.13 (104)a Middle 1 2 3 Mean 3.44 3.19 3.32 3.32 (111) Bottom 1 2 3 Mean 3.06 3.98 3.34 3.46 (115) a Each value in parentheses is the percent of theoretical. 105 Covance 6329-212 3M T-6316.1 Table 1 (Continued) Results of Homogeneity Analyses (ppm) Mixed 11/7/97 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Sample Location Replicate 3 T-6316 (ppm) 300 Top 1 2.73 296 2 3.19 305 3 Mean 3.53 302 3.15 (105)a 301 (100) Middle 1 2 3 Mean 3.67 2.51 2.83 3.00 (100) 304 300 297 300 (100) Bottom 1 2 3 Mean 3.12 2.91 3.12 3.05 (102) 293 278 328 300 (100) a Each value in parentheses is the percent of theoretical. 106 Covance 6329-212 3M T-6316.1 Table 1 (Continued) Results of Homogeneity Analyses (ppm) Mixed 11/13/97 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Sample Location Replicate 3 T-6316 (ppm) 300 Top 1 2.93 290 2 2.86 314 3 Mean 2.91 291 2.90 (96.7)a 298 (99.3) Middle 1 2 3 Mean 2.65 2.90 2.87 2.81 (93.7) 288 302 293 294 (98.0) Bottom 1 2 3 Mean 2.66 2.84 3.40 2.97 (99.0) 284 269 308 287 (95.7) a Each value in parentheses is the percent of theoretical. 107 Covance 6329-212 3M T-6316.1 Table 1 (Continued) Results of Homogeneity Analyses (ppm) Mixed 1/15/98 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Sample Location Replicate 3 T-6316 (ppm) 30 100 300 Top 1 2.89 31.8 89.4 275 2 2.54 27.9 93.7 278 3 Mean 2.63 29.0 2.69 (89.7)a 29.6 (98.7) 99.2 294 94.1 (94.1) 282 (94.0) Middle 1 2 3 Mean 2.67 2.66 2.83 2.72 (90.7) 28.9 29.8 27.2 28.6 (95.3) 87.4 82.2 90.8 86.8 (86.8) 280 300 289 290 (96.7) Bottom Bottomb 1 2 3 Mean 1 2 3 Mean 3.02 8.49 3.07 4.86 (162) 3.68 2.52 3.19 3.13 (104) 27.9 27.0 28.0 27.6 (92.0) 89.1 101 105 98.4 (98.4) 291 283 278 284 (94.7) - - - - - - - - - - - - a Each value in parentheses is the percent of theoretical. b Reassay. 108 Covance 6329-212 3M T-6316.1 Table 1 (Continued) Results of Homogeneity Analyses (ppm) Mixed 8/26/99 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS T-6316 (ppm) Sample Location Replicate 100 Top 1 156 2 Mean 115 136 (136)a Middle 1 2 Mean 121 121 121 (121) Bottom 1 2 Mean 123 145 134 (134) a Each value in parentheses is the percent of theoretical. 109 Covance 6329-212 3M T-6316.1 Table 1 (Continued) Results of Homogeneity Analyses (ppm) Mixed 8/26/99 (Reanalysis) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS T-6316 (ppm) Sample Location Replicate 100 Top 1 76.5 2 Mean 66.0 71.3 (71.3)a Middle 1 2 Mean 66.0 79.4 72.7 (72.7) Bottom 1 2 Mean 63.5 68.9 66.2 (66.2) a Each value in parentheses is the percent of theoretical. 110 Covance 6329-212 3M T-6316.1 Table 2 Results of Stability Analyses (ppm) Mixed 9/11/97 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Storage Conditions Replicate 3 T-6316 (ppm) 300 Initial 1 2 Mean 7.57 4.70 6.14 (205)a 271 292 282 (94.0) 19 days, room temperature 1 - 2 - Mean - 215 207 211 (70.3) a Each value in parentheses is the percent of theoretical. 111 Covance 6329-212 3M T-6316.1 Table 2 (Continued) Results of Stability Analyses (ppm) Mixed 10/15/97 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Storage Conditions Replicate 3 T-6316 (ppm) 300 Initiala 1 2 Mean 3.44 3.19 3.32 (111)b 302 292 297 (99.0) 19 days, room temperature 1 2 Mean 2.83 3.13 2.98 (99.3) 250 270 260 (86.7) 34 days, room temperature 1 2 Mean 2.90 3.09 3.00 (100) 266 266 266 (88.7) 8 weeks, frozen 1 2 Mean 3.13 2.80 2.97 (99.0) 275 291 283 (94.3) a Results were derived from the sample collected from the middle of the dose preparations for homogeneity analysis. b Each value in parentheses is the percent of theoretical. 112 Covance 6329-212 3M T-6316.1 Table 2 (Continued) Results of Stability Analyses (ppm) Mixed 9/29/97 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS T-6316 (ppm) Storage Conditions Replicate 3 Initial 1 2 Mean 4.00 4.47 4.24 (141)a a Each value in parentheses is the percent of theoretical. 113 Covance 6329-212 3M T-6316.1 Table 2 (Continued) Results of Stability Analyses (ppm) Mixed 11/13/97 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Storage Conditions Replicate 3 T-6316 (ppm) 300 5 days, room temperature 7 days, animal room conditions 1 2 3 Mean 4.16 3.42 3.00 3.53 (118)a 264 258 267 263 (87.7) a Each value in parentheses is the percent of theoretical. 114 Covance 6329-212 3M T-6316.1 Table 3 Results of Dose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Week 1-3a Replicate 0 1 b 2 b 3 - Mean - T-6316 (ppm) 3 30 100 300 2.67 2.66 2.83 2.72 (90.7)c 28.9 29.8 27.2 28.6 (95.3) 87.4 82.2 90.8 86.8 (86.8) 280 300 289 290 (96.7) 4-7 1 b 5.64 29.0 90.4 292 2 b 3.10 27.0 97.5 301 Mean - 4.37 (146) 28.0 (93.3) 94.0 (94.0) 297 (99.0) 4-7d 1 - 2.81 - - 2 - 3.05 - - - Mean - 2.93 (97.7) - - - 8-11 8-11d 1 b 2 b Mean - 1 - 2 - Mean - 4.53 3.45 3.99 (133) 2.66 3.20 2.93 (97.7) 29.9 28.1 29.0 (96.7) 84.3 86.5 85.4 (85.4) 278 262 270 (90.0) - - - - - - - - - 12-15 1 b 2 b Mean - 3.34 2.67 3.01 (101) 26.9 35.6 31.3 (104) 98.2 - 99.1 - 98.7 (98.7) - a Results for the 3-, 30-, 100-, and 300-ppm levels were derived from the sample collected from the middle of the dose preparations for homogeneity analysis. b Below the limit of quantitation (<2.00 ppm). c Each value in parentheses is the percent of theoretical. d Reassay. 115 Covance 6329-212 3M T-6316.1 Table 3 (Continued) Results of Dose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Week Replicate 0 3 T-6316 (ppm) 30 100 300 16-19 16-19d 16-19f 1 e 3.77 39.3 87.2 - 2 e 3.04 31.5 93.0 - Mean - 3.41 (114)c 35.4 (118) 90.1 (90.1) - 1 - 2.78 21.8 - - 2 - 2.20 44.6 - - Mean - 2.49 (83.0) 33.2 (111) - - 1 - 2.62 26.9 - - 2 - 2.52 23.3 - - Mean - 2.57 (85.7) 25.1 (83.7) - - 20-23 20-23d 20-23f 1 e 2.61 27.7 101 - 2 e 2.41 26.4 92.3 - Mean - 2.51 (83.7) 27.1 (90.3) 96.7 (96.7) - 1 - 2.15 - 2 - 2.47 - Mean - 2.31 (77.0) - - - - - - - 1 - 2.14 - 2 - 1.90 - Mean - 2.02 (67.3) - - - - - - - c Each value in parentheses is the percent of theoretical. d Reassay e Below the limit of quantitation (<0.5 ppm) f Retention. 116 Covance 6329-212 3M T-6316.1 Table 3 (Continued) Results of Dose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Week Replicate 0 3 T-6316 (ppm) 30 100 300 24-27 24-27d 24-27f 1 e 2 e Mean - 1 - 2 - Mean - 1 - 2 - Mean - 2.63 24.1 83.3 - 3.04 26.7 88.4 - 2.84 (94.7)c 25.4 (84.7 ) 85.9 (85.9) - - 25.3 - - - 24.0 - - - 24.7 (82.3)c - - - 22.4 - - - 21.4 - - - 21.9 (73.0) - - 28-31 28-31d 28-31f 1 e 2 e Mean - 1 - 2 - Mean - 1 - 2 - Mean - 2.24 26.4 87.5 - 2.52 28.4 86.0 - 2.38 (79.3) 27.4 (91.3) 86.8 (86.8) - 3.20 - 2.60 - 2.90 (96.7) - - - - - - - 3.20 - 2.73 - 2.97 (99.0) - - - - - - - 32-35 1 e 2 e Mean - 2.92 28.2 83.4 - 2.46 29.7 85.9 - 2.69 (89.7) 29.0 (96.7) 84.7 (84.7) - c Each value in parentheses is the percent of theoretical. d Reassay. e Below the limit of quantitation (<0.5 ppm) f Retention. 117 Covance 6329-212 3M T-6316.1 Table 3 (Continued) Results of Dose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Week Replicate 0 3 T-6316 (ppm) 30 100 300 36-39 36-39d 36-39f 1 e 2 e Mean - 1 - 2 - Mean - 1 - 2 - Mean - 3.36 24.9 91.4 - 2.58 22.7 87.2 - 2.97 (99.0)c 23.8 (79.3) 89.3 (89.3) - 2.53 23.0 - - 2.51 22.8 - - 2.52 (84.0) 22.9 (76.3) - - 2.82 27.0 - - 2.68 29.6 - - 2.75 (91.7) 28.3 (94.3) - - 40-43 40-43d 1 e 2 e Mean - 1 - 2 - Mean - 3.01 28.9 95.5 - 5.26 28.3 99.5 - 4.14 (138) 28.6 (95.3) 97.5 (97.5) - 2.73 - 2.71 - 2.72 (90.7) - - - - - - - 44-47 44-47d 1 e 2 e Mean - 1 - 2 - Mean - 3.97 29.3 84.4 - 2.77 27.9 95.8 - 3.37 (112) 28.6 (95.3) 90.1 (90.1) - 2.93 - 2.04 - 2.49 (83.0) - 64.0 - 59.6 - 61.8 (61.8) - c Each value in parentheses is the percent of theoretical. d Reassay. e Below the limit of quantitation (<0.5 ppm). f Retention. 118 Covance 6329-212 3M T-6316.1 Table 3 (Continued) Results of Dose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Week 44-47f Replicate 0 1 - 2 - Mean - T-6316 (ppm) 3 30 100 300 5.30 - 2.75 - 4.03 (134)c - 60.4 - 58.4 - 64.4 (64.4) - 48-51 1 e 2 e Mean - 2.67 30.2 97.8 - 2.59 29.0 94.7 - 2.63 (87.7) 29.6 (98.7) 96.3 (96.3) - 52-55 52-55d 52-55f 52-55g 1 e 2 e Mean - 1 - 2 - Mean - 1 - 2 - Mean - 1 - 2 - Mean - 3.22 3.45 3.34 (111) 25.2 92.5 - 24.0 97.6 - 24.6 (82.0) 95.1 (95.1) - - 20.4 - - - 21.0 - - - 20.7 (69.0) - - - 19.0 - - - 21.5 - - - 20.3 (67.7) - - - - - - - - 88.4 - 84.5 - 86.5 (86.5) - c Each value in parentheses is the percent of theoretical. d Reassay. e Below the limit of quantitation (<0.5 ppm). f Retention. g Additional mix. 119 Covance 6329-212 3M T-6316.1 Table 3 (Continued) Results of Dose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Week Replicate 0 3 T-6316 (ppm) 30 100 300 56-59 56-59d 56-59f 1 e 2 e Mean - 1 - 2 - Mean - 1 - 2 - Mean - 3.16 49.3 114 - 2.43 49.0 90.6 - 2.80 (93.3)c 49.2 (164) 102 (102) - 1.71 1.70 1.71 (57.0) 16.0 15.6 15.8 (52.7) 56.0 - 58.2 - 57.1 (57.1) - 1.75 1.72 1.74 (58.0) 18.6 17.8 18.2 (60.7) 56.8 - 54.4 - 55.6 (55.6) - 60-63 60-63d 60-63f 1 e 2 e Mean - 1 - 2 - Mean - 1 - 2 - Mean - 2.46 2.56 2.51 (83.7) 28.2 26.4 27.3 (91.0) 2.48 - 2.38 - 2.43 (81.0) - 34.8 - 3.85 - 19.3 (643) - 87.6 - 83.2 - 85.4 (85.4) - - - - - - - - - - - - - 64-67 1 e 2 e Mean - 2.45 1.97 2.21 (73.7) 17.7 28.0 22.9 (76.3) 73.7 - 73.4 - 73.6 (73.6) - c Each value in parentheses is the percent of theoretical. d Reassay. e Below the limit of quantitation (<0.5 ppm). f Retention. 120 Covance 6329-212 3M T-6316.1 Table 3 (Continued) Results of Dose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Week 64-67d 64-67f Replicate 0 1 - 2 - Mean - 1 - 2 - Mean - T-6316 (ppm) 3 30 100 300 1.59 1.58 1.59(53.0)c 20.1 18.4 19.3 (64.3) 63.6 - 66.2 - 64.9 (64.9) - 1.46 3.04 2.25 (75.0) 14.9 13.6 14.3 (47.7) 55.8 - 64.4 - 60.1 (60.1) - 68-71 68-71d 68-71f 1 e 2 e Mean - 1 - 2 - Mean - 1 - 2 - Mean - 2.51 25.3 2.18 24.2 2.35 (78.3)c 24.8 (82.7) 2.12 2.23 2.18 (72.7) 24.3 20.6 22.5 (75.0) 2.31 6.00 4.16 (139) 20.7 24.5 22.6 (75.3) 82.5 - 82.3 - 82.4 (82.4) - 80.2 - 84.1 - 82.2 (82.2) - 61.6 - 62.1 - 61.9 (61.9) - 72-75 1 2 Mean 1.11 0.623 0.867 2.63 1.98 2.31 (77.0) 24.0 28.5 26.3 (87.7) 86.9 - 80.8 - 83.9 (83.9) - c Each value in parentheses is the percent of theoretical. d Reassay. e Below the limit of quantitation (<0.5 ppm) f Retention. 121 Covance 6329-212 3M T-6316.1 Table 3 (Continued) Results of Dose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Week 72-75d 72-75f Replicate 0 1 e 2 e Mean - 1 e 2 e Mean - T-6316 (ppm) 3 30 100 300 2.02 22.4 2.25 21.7 2.14 (71.3)c 22.1 (73.7) 89.0 - 85.8 - 87.4 (87.4) - 2.18 21.4 - - 1.96 20.2 - - 2.07 (69.0) 20.8 (69.3) - - 76-79 76-79d 1 e 2 e Mean - 1 e 2 e Mean - 2.69 2.42 2.56 (85.3) 25.8 30.6 28.2 (94.0) - 23.1 - 23.4 - 23.3 (77.7) 82.0 - 93.8 - 87.9 (87.9) - - - - - - - 80-83 1 e 2 e Mean - 2.35 2.60 2.48 (82.7) 27.2 24.0 25.6 (85.3) 92.8 - 86.0 - 89.4 (89.4) - 84-87 1 e 2 e Mean - 3.67 35.1 121 - 3.86 44.7 121 - 3.77 (126) 39.9 (133) 121 (121) - c Each value in parentheses is the percent of theoretical. d Reassay. e Below the limit of quantitation (<0.5 ppm) f Retention. 122 Covance 6329-212 3M T-6316.1 Table 3 (Continued) Results of Dose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS T-6316 (ppm) Week Replicate 0 3 30 100 300 88-91 1 e 2 e Mean - 2.55 2.49 2.52 (84.0)c 32.4 28.2 30.3 (101) 94.2 - 92.4 - 93.3 (93.3) - 96-99 1 e 2 e Mean - 2.38 2.44 2.41 (80.3) 34.9 29.7 32.3 (108) 97.4 - 88.8 - 93.1 (93.1) - 100-103 100-103d 1 e 2 e Mean - 1 - 2 - Mean - 2.31 3.41 2.86 (95.3) 1.09 0.914 1.00 (33.3) 36.1 25.5 30.8 (103) 68.9 25.6 47.3 (158) 75.3 - 90.0 - 82.7 (82.7) - 59.7 - 61.0 - 60.4 (60.4) - 104-105 104-105d 104-105d 1 e 2 e Mean - 1 - 2 - Mean - 1 - 2 - Mean - 4.30 2.26 3.28 (109) 1.89 1.88 1.89 (63.0) 1.79 1.82 1.81 (60.3) 26.1 91.1 - 28.1 95.7 - 27.1 (90.3) 93.4 (93.4) - - - - - - - - - - - - - - - - - - - c Each value in parentheses is the percent of theoretical. d Reassay. e Below the limit of quantitation (<0.5 ppm). f Retention. 123 Table 4 Survival Data Covance 6329-212 3M T-6316.1 PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: START 1 2 3 4 5 6 7 8 9 10 11 ---------------------------------------------------------------------------------------------------------------------- MALE 1 0 70/70 70/70 70/70 70/70 65/65 65/65 65/65 65/65 60/60 60/60 60/60 60/60 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 2 3 60/60 60/60 60/60 60/60 55/55 55/55 55/55 55/55 54/55 54/55 54/55 54/55 100% 100% 100% 100% 100% 100% 100% 100% 98% 98% 98% 98% 3 30 60/60 60/60 60/60 60/60 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 4 100 70/70 70/70 70/70 70/70 65/65 65/65 65/65 65/65 65/65 65/65 65/65 65/65 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 5 300 70/70 70/70 70/70 70/70 65/65 65/65 65/65 63/65 0 0 0 0 100% 100% 100% 100% 100% 100% 100% 97% 0% 0% 0% 0% 6 100 40/40 40/40 40/40 40/40 40/40 40/40 40/40 39/40 39/40 39/40 39/40 39/40 100% 100% 100% 100% 100% 100% 100% 98% 98% 98% 98% 98% 7 300 40/40 40/40 40/40 40/40 40/40 40/40 39/40 39/40 0 0 0 0 100% 100% 100% 100% 100% 100% 98% 98% 0% 0% 0% 0% 124 Table 4 Survival Data Covance 6329-212 3M T-6316.1 PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 12 13 14 15 16 17 18 19 20 21 22 23 ---------------------------------------------------------------------------------------------------------------------- MALE 1 0 60/60 60/60 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 2 3 54/55 54/55 49/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 3 30 55/55 55/55 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 4 100 65/65 64/65 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 100% 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 5 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 6 100 38/40 38/40 38/40 38/40 38/40 38/40 38/40 38/40 38/40 38/40 38/40 38/40 95% 95% 95% 95% 95% 95% 95% 95% 95% 95% 95% 95% 7 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 125 Table 4 Survival Data Covance 6329-212 3M T-6316.1 PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 24 25 26 27 28 29 30 31 32 33 34 35 ---------------------------------------------------------------------------------------------------------------------- MALE 1 0 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 2 3 49/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 48/50 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 96% 3 30 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 4 100 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 5 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 6 100 38/40 38/40 38/40 38/40 38/40 38/40 38/40 38/40 38/40 38/40 38/40 38/40 95% 95% 95% 95% 95% 95% 95% 95% 95% 95% 95% 95% 7 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 126 Table 4 Survival Data Covance 6329-212 3M T-6316.1 PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 36 37 38 39 40 41 42 43 44 45 46 47 ---------------------------------------------------------------------------------------------------------------------- MALE 1 0 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 54/55 54/55 54/55 54/55 100% 100% 100% 100% 100% 100% 100% 100% 98% 98% 98% 98% 2 3 48/50 48/50 48/50 48/50 48/50 48/50 48/50 48/50 48/50 48/50 48/50 48/50 96% 96% 96% 96% 96% 96% 96% 96% 96% 96% 96% 96% 3 30 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 4 100 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 58/60 58/60 58/60 98% 98% 98% 98% 98% 98% 98% 98% 98% 97% 97% 97% 5 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 6 100 38/40 38/40 38/40 38/40 38/40 38/40 38/40 37/40 37/40 37/40 37/40 37/40 95% 95% 95% 95% 95% 95% 95% 93% 93% 93% 93% 93% 7 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 127 Table 4 Survival Data Covance 6329-212 3M T-6316.1 PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 48 49 50 51 52 53 54 55 56 57 58 59 ---------------------------------------------------------------------------------------------------------------------- MALE 1 0 54/55 54/55 54/55 54/55 54/55 49/50 49/50 49/50 49/50 49/50 49/50 49/50 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 2 3 48/50 48/50 48/50 48/50 48/50 48/50 48/50 48/50 48/50 47/50 46/50 45/50 96% 96% 96% 96% 96% 96% 96% 96% 96% 94% 92% 90% 3 30 50/50 50/50 50/50 50/50 50/50 49/50 49/50 49/50 48/50 48/50 48/50 48/50 100% 100% 100% 100% 100% 98% 98% 98% 96% 96% 96% 96% 4 100 58/60 58/60 58/60 58/60 58/60 48/50 48/50 48/50 48/50 48/50 48/50 47/50 97% 97% 97% 97% 97% 96% 96% 96% 96% 96% 96% 94% 5 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 6 100 37/40 37/40 37/40 36/40 36/40 36/40 36/40 36/40 36/40 36/40 36/40 36/40 93% 93% 93% 90% 90% 90% 90% 90% 90% 90% 90% 90% 7 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 128 Table 4 Survival Data Covance 6329-212 3M T-6316.1 PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 60 61 62 63 64 65 66 67 68 69 70 71 ---------------------------------------------------------------------------------------------------------------------- MALE 1 0 49/50 48/50 47/50 46/50 46/50 45/50 44/50 44/50 44/50 43/50 42/50 42/50 98% 96% 94% 92% 92% 90% 88% 88% 88% 86% 84% 84% 2 3 45/50 44/50 44/50 44/50 44/50 44/50 44/50 44/50 43/50 43/50 42/50 41/50 90% 88% 88% 88% 88% 88% 88% 88% 86% 86% 84% 82% 3 30 48/50 48/50 47/50 47/50 47/50 47/50 47/50 46/50 46/50 43/50 43/50 42/50 96% 96% 94% 94% 94% 94% 94% 92% 92% 86% 86% 84% 4 100 47/50 47/50 47/50 47/50 45/50 45/50 45/50 45/50 45/50 42/50 42/50 41/50 94% 94% 94% 94% 90% 90% 90% 90% 90% 84% 84% 82% 5 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 6 100 36/40 35/40 35/40 35/40 34/40 34/40 34/40 34/40 33/40 33/40 33/40 33/40 90% 88% 88% 88% 85% 85% 85% 85% 83% 83% 83% 83% 7 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 129 Table 4 Survival Data Covance 6329-212 3M T-6316.1 PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 72 73 74 75 76 77 78 79 80 81 82 83 ---------------------------------------------------------------------------------------------------------------------- MALE 1 0 40/50 40/50 40/50 40/50 40/50 39/50 39/50 37/50 36/50 36/50 36/50 36/50 80% 80% 80% 80% 80% 78% 78% 74% 72% 72% 72% 72% 2 3 41/50 41/50 40/50 40/50 40/50 39/50 38/50 38/50 37/50 34/50 34/50 33/50 82% 82% 80% 80% 80% 78% 76% 76% 74% 68% 68% 66% 3 30 41/50 39/50 39/50 39/50 38/50 37/50 36/50 35/50 34/50 32/50 32/50 32/50 82% 78% 78% 78% 76% 74% 72% 70% 68% 64% 64% 64% 4 100 41/50 40/50 38/50 38/50 38/50 38/50 37/50 36/50 36/50 36/50 35/50 35/50 82% 80% 76% 76% 76% 76% 74% 72% 72% 72% 70% 70% 5 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 6 100 33/40 32/40 32/40 32/40 32/40 32/40 31/40 31/40 30/40 30/40 29/40 26/40 83% 80% 80% 80% 80% 80% 78% 78% 75% 75% 73% 65% 7 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 130 Table 4 Survival Data Covance 6329-212 3M T-6316.1 PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 84 85 86 87 88 89 90 91 92 93 94 95 ---------------------------------------------------------------------------------------------------------------------- MALE 1 0 35/50 34/50 33/50 31/50 30/50 29/50 29/50 29/50 28/50 24/50 23/50 22/50 70% 68% 66% 62% 60% 58% 58% 58% 56% 48% 46% 44% 2 3 33/50 33/50 32/50 32/50 32/50 32/50 32/50 32/50 30/50 28/50 27/50 27/50 66% 66% 64% 64% 64% 64% 64% 64% 60% 56% 54% 54% 3 30 32/50 32/50 32/50 32/50 32/50 32/50 32/50 29/50 29/50 29/50 28/50 27/50 64% 64% 64% 64% 64% 64% 64% 58% 58% 58% 56% 54% 4 100 35/50 34/50 32/50 32/50 31/50 31/50 30/50 30/50 29/50 28/50 27/50 27/50 70% 68% 64% 64% 62% 62% 60% 60% 58% 56% 54% 54% 5 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 6 100 24/40 22/40 21/40 21/40 21/40 20/40 19/40 19/40 19/40 16/40 16/40 16/40 60% 55% 53% 53% 53% 50% 48% 48% 48% 40% 40% 40% 7 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 131 Table 4 Survival Data Covance 6329-212 3M T-6316.1 PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 96 97 98 99 100 101 102 103 104 ---------------------------------------------------------------------------------------------------------------------- MALE 1 0 21/50 20/50 19/50 18/50 15/50 15/50 14/50 14/50 14/50 42% 40% 38% 36% 30% 30% 28% 28% 28% 2 3 27/50 27/50 25/50 24/50 23/50 22/50 22/50 22/50 22/50 54% 54% 50% 48% 46% 44% 44% 44% 44% 3 30 27/50 26/50 25/50 24/50 24/50 24/50 24/50 23/50 22/50 54% 52% 50% 48% 48% 48% 48% 46% 44% 4 100 26/50 26/50 25/50 25/50 23/50 21/50 20/50 18/50 17/50 52% 52% 50% 50% 46% 42% 40% 36% 34% 5 300 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 6 100 16/40 15/40 14/40 14/40 12/40 12/40 11/40 10/40 10/40 40% 38% 35% 35% 30% 30% 27% 25% 25% 7 300 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 132 Table 4 Survival Data Covance 6329-212 3M T-6316.1 PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: START 1 2 3 4 5 6 7 8 9 10 11 ---------------------------------------------------------------------------------------------------------------------- FEMALE 1 0 70/70 70/70 70/70 70/70 65/65 65/65 65/65 65/65 60/60 60/60 60/60 60/60 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 2 3 60/60 60/60 60/60 60/60 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 3 30 60/60 60/60 60/60 60/60 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 4 100 70/70 70/70 70/70 70/70 65/65 65/65 65/65 65/65 65/65 65/65 65/65 65/65 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 5 300 70/70 70/70 70/70 70/70 65/65 65/65 65/65 65/65 0 0 0 0 100% 100% 100% 100% 100% 100% 100% 100% 0% 0% 0% 0% 6 100 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 7 300 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 0 0 0 0 100% 100% 100% 100% 100% 100% 100% 100% 0% 0% 0% 0% 133 Table 4 Survival Data Covance 6329-212 3M T-6316.1 PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 12 13 14 15 16 17 18 19 20 21 22 23 ---------------------------------------------------------------------------------------------------------------------- FEMALE 1 0 60/60 60/60 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 2 3 55/55 55/55 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 3 30 55/55 55/55 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 4 100 65/65 65/65 60/60 59/59 59/59 59/59 59/59 59/59 59/59 59/59 58/59 58/59 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 98% 98% 5 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 6 100 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 7 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 134 Table 4 Survival Data Covance 6329-212 3M T-6316.1 PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 24 25 26 27 28 29 30 31 32 33 34 35 ---------------------------------------------------------------------------------------------------------------------- FEMALE 1 0 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 2 3 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 3 30 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 4 100 58/59 58/59 58/59 58/59 58/59 58/59 58/59 58/59 58/59 58/59 58/59 58/59 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 5 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 6 100 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 7 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 135 Table 4 Survival Data Covance 6329-212 3M T-6316.1 PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 36 37 38 39 40 41 42 43 44 45 46 47 ---------------------------------------------------------------------------------------------------------------------- FEMALE 1 0 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 54/55 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 98% 2 3 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 3 30 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 4 100 58/59 58/59 58/59 58/59 58/59 58/59 58/59 58/59 58/59 58/59 58/59 58/59 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 5 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 6 100 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 7 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 136 Table 4 Survival Data Covance 6329-212 3M T-6316.1 PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 48 49 50 51 52 53 54 55 56 57 58 59 ---------------------------------------------------------------------------------------------------------------------- FEMALE 1 0 54/55 54/55 54/55 54/55 54/55 49/50 49/50 49/50 49/50 49/50 48/50 48/50 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 96% 96% 2 3 50/50 50/50 50/50 50/50 50/50 50/50 49/50 49/50 49/50 49/50 48/50 48/50 100% 100% 100% 100% 100% 100% 98% 98% 98% 98% 96% 96% 3 30 50/50 50/50 50/50 50/50 50/50 49/50 49/50 49/50 49/50 49/50 49/50 47/50 100% 100% 100% 100% 100% 98% 98% 98% 98% 98% 98% 94% 4 100 58/59 58/59 58/59 58/59 58/59 48/49 48/49 48/49 48/49 48/49 48/49 47/49 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 96% 5 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 6 100 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 7 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 137 Table 4 Survival Data Covance 6329-212 3M T-6316.1 PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 60 61 62 63 64 65 66 67 68 69 70 71 ---------------------------------------------------------------------------------------------------------------------- FEMALE 1 0 48/50 47/50 46/50 46/50 45/50 45/50 45/50 44/50 44/50 44/50 44/50 44/50 96% 94% 92% 92% 90% 90% 90% 88% 88% 88% 88% 88% 2 3 48/50 47/50 47/50 47/50 47/50 47/50 46/50 44/50 42/50 42/50 42/50 42/50 96% 94% 94% 94% 94% 94% 92% 88% 84% 84% 84% 84% 3 30 47/50 47/50 47/50 46/50 46/50 46/50 46/50 44/50 44/50 42/50 41/50 41/50 94% 94% 94% 92% 92% 92% 92% 88% 88% 84% 82% 82% 4 100 47/49 47/49 47/49 46/49 45/49 45/49 45/49 45/49 45/49 44/49 43/49 42/49 96% 96% 96% 94% 92% 92% 92% 92% 92% 90% 88% 86% 5 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 6 100 40/40 40/40 39/40 38/40 37/40 37/40 37/40 37/40 37/40 36/40 36/40 35/40 100% 100% 98% 95% 93% 93% 93% 93% 93% 90% 90% 88% 7 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 138 Table 4 Survival Data Covance 6329-212 3M T-6316.1 PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 72 73 74 75 76 77 78 79 80 81 82 83 ---------------------------------------------------------------------------------------------------------------------- FEMALE 1 0 44/50 42/50 41/50 37/50 36/50 34/50 33/50 33/50 32/50 31/50 31/50 31/50 88% 84% 82% 74% 72% 68% 66% 66% 64% 62% 62% 62% 2 3 42/50 40/50 40/50 36/50 35/50 34/50 34/50 33/50 33/50 33/50 33/50 32/50 84% 80% 80% 72% 70% 68% 68% 66% 66% 66% 66% 64% 3 30 38/50 36/50 34/50 34/50 34/50 34/50 34/50 34/50 34/50 33/50 33/50 30/50 76% 72% 68% 68% 68% 68% 68% 68% 68% 66% 66% 60% 4 100 41/49 41/49 39/49 38/49 38/49 38/49 36/49 35/49 35/49 35/49 35/49 34/49 84% 84% 80% 78% 78% 78% 73% 71% 71% 71% 71% 69% 5 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 6 100 34/40 33/40 31/40 31/40 31/40 30/40 30/40 30/40 29/40 28/40 28/40 26/40 85% 83% 78% 78% 78% 75% 75% 75% 73% 70% 70% 65% 7 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 139 Table 4 Survival Data Covance 6329-212 3M T-6316.1 PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 84 85 86 87 88 89 90 91 92 93 94 95 ---------------------------------------------------------------------------------------------------------------------- FEMALE 1 0 30/50 30/50 29/50 29/50 27/50 25/50 23/50 22/50 22/50 21/50 19/50 18/50 60% 60% 58% 58% 54% 50% 46% 44% 44% 42% 38% 36% 2 3 31/50 30/50 29/50 27/50 26/50 26/50 26/50 25/50 24/50 24/50 22/50 22/50 62% 60% 58% 54% 52% 52% 52% 50% 48% 48% 44% 44% 3 30 30/50 30/50 28/50 27/50 27/50 26/50 26/50 25/50 25/50 24/50 22/50 22/50 60% 60% 56% 54% 54% 52% 52% 50% 50% 48% 44% 44% 4 100 34/49 33/49 31/49 30/49 29/49 29/49 29/49 29/49 29/49 28/49 27/49 27/49 69% 67% 63% 61% 59% 59% 59% 59% 59% 57% 55% 55% 5 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 6 100 26/40 25/40 23/40 22/40 22/40 22/40 21/40 21/40 20/40 17/40 17/40 17/40 65% 63% 58% 55% 55% 55% 53% 53% 50% 43% 43% 43% 7 300 0 0 0 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 140 Table 4 Survival Data Covance 6329-212 3M T-6316.1 PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 96 97 98 99 100 101 102 103 104 ---------------------------------------------------------------------------------------------------------------------- FEMALE 1 0 17/50 17/50 15/50 15/50 15/50 15/50 15/50 13/50 13/50 34% 34% 30% 30% 30% 30% 30% 26% 26% 2 3 21/50 17/50 16/50 15/50 14/50 14/50 14/50 14/50 13/50 42% 34% 32% 30% 28% 28% 28% 28% 26% 3 30 22/50 20/50 19/50 18/50 17/50 17/50 17/50 16/50 15/50 44% 40% 38% 36% 34% 34% 34% 32% 30% 4 100 27/49 25/49 23/49 23/49 23/49 21/49 21/49 19/49 19/49 55% 51% 47% 47% 47% 43% 43% 39% 39% 5 300 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 6 100 15/40 14/40 13/40 13/40 12/40 12/40 12/40 12/40 12/40 38% 35% 33% 33% 30% 30% 30% 30% 30% 7 300 0 0 0 0 0 0 0 0 0 0% 0% 0% 0% 0% 0% 0% 0% 0% 141 Table 5 Covance 6329-212 3M T-6316.1 Summary of Clinical Observations PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: ---------------MALE-------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ *** TOP OF LIST *** APPEARANCE BENT TAIL 0000000 CONVULSIONS 0001210 DEHYDRATED 0000001 HEAD TILT LEFT 0002010 RIGHT 1010040 HUNCHED 9 6 7 13 0 4 0 LIMITED USE LIMB-HIND-RIGHT 1000010 LIMBS-FRONT 1000000 LIMBS-HIND 3001000 PAW-HIND-LEFT 0000000 PAW-HIND-RIGHT 1000000 PAWS-FRONT 0001000 PAWS-HIND 1000000 MALOCCLUSION 5 5 9 23 2 9 2 MISSING TAIL-PROXIMAL 0000000 TEETH 1114010 OBESE 0100000 PARALYSIS LIMB-FRONT-LEFT 0001010 LIMBS-HIND 0110000 PROLAPSED VAGINA 0000000 142 Table 5 Covance 6329-212 3M T-6316.1 Summary of Clinical Observations PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: ---------------MALE-------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ APPEARANCE PROTRUDING PENIS SWOLLEN DORSAL-CERVICAL INGUINAL REGION-LEFT INGUINAL REGION-RIGHT LIMB-HIND-LEFT LIMB-HIND-RIGHT LIMBS-HIND MOUTH NOSE PAW-FRONT-LEFT PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-FRONT PAWS-HIND TAIL-PROXIMAL VENTRAL-ABDOMINAL VENTRAL-CERVICAL THIN EXCESSIVE BLEEDING FROM PAWHR, CAUTERIZED WITH SILVER NITRATE 2121000 1000000 0000000 0000000 2100000 0100000 1000000 0001030 0100000 0000000 3401020 1010010 0001000 1211000 0001000 0000010 1000000 18 15 15 20 1 11 1 1000000 BEHAVIOR ATAXIC CIRCLING LEFT 1025010 0000000 143 Table 5 Covance 6329-212 3M T-6316.1 Summary of Clinical Observations PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: ---------------MALE-------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ BEHAVIOR CIRCLING RIGHT HYPOACTIVE RECUMBENT LATERAL STERNAL SENSITIVE TO VOCALIZATION TOUCH 0000000 15 8 11 15 1 8 1 5322010 2221020 2421030 0000100 DISCHARGE GENITAL RED IN MUCOID ORAL RED IN CLEAR NASAL RED IN UNKNOWN RED IN COLOR COLOR COLOR COLOR 1212040 0000000 2102001 0100010 3337140 0000100 EXCRETION DISCOLORED URINE RED IN COLOR ORANGE IN COLOR 1001000 1000000 144 Table 5 Covance 6329-212 3M T-6316.1 Summary of Clinical Observations PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: ---------------MALE-------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ EXCRETION FEW FECES LIQUID FECES NO FECES NONFORMED FECES 16 16 15 14 0 14 1 0000010 10 4 9 9 0 5 0 9 10 8 6 0 2 0 EYES CLEAR DISCHARGE EYE-LEFT EYE-RIGHT EYES CLOUDY DISCHARGE EYE-RIGHT DISCOLORED/DARK RED EYE-LEFT DILATED PUPIL EYES DISCOLORED/LIGHT RED EYE-LEFT EYE-RIGHT EYES OPAQUE EYE-LEFT EYE-RIGHT EYES 1000000 0000000 1000000 0000000 0000000 0100000 1101000 0000010 0011000 0100000 1001000 1200000 145 Table 5 Covance 6329-212 3M T-6316.1 Summary of Clinical Observations PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: ---------------MALE-------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ EYES PALE EYE-RIGHT EYES RED DISCHARGE EYE-LEFT EYE-RIGHT EYES SQUINTED PERI-ORBITAL PERI-ORBITAL-RIGHT 1000000 2100010 2032030 4202030 9357050 5248050 0010000 RESPIRATION AUDIBLE IRREGULAR LABORED 2333000 1321030 6632130 SKIN & PELAGE ALOPECIA BROWN HAIR COAT HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT PERINEAL AREA SCROTUM VENTRAL-ABDOMINAL GENERALIZED 0000000 0001000 0001000 3322040 0101000 2316040 0111020 146 Table 5 Covance 6329-212 3M T-6316.1 Summary of Clinical Observations PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: ---------------MALE-------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ SKIN & PELAGE BLUE SKIN PAW-HIND-LEFT VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL BROWN SKIN PAWS-ALL VENTRAL-ABDOMINAL COLD TO TOUCH LIMBS-ALL LIMBS-HIND ENTIRE BODY BLACK SKIN VENTRAL-ABDOMINAL-LEFT VENTRAL-THORACIC-RIGHT PALE EARS ENTIRE BODY RED HAIR COAT DORSAL-CERVICAL DORSAL-CERVICAL-RIGHT HEAD-ENTIRE HEAD-CRANIAL HEAD-MAXILLARY-LEFT 0100000 0000000 0000000 0000000 0000000 0010000 0001000 0000000 0000000 3103120 0000000 0000000 0000000 2123120 0000000 0000000 4475120 3121000 2000000 147 Table 5 Covance 6329-212 3M T-6316.1 Summary of Clinical Observations PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: ---------------MALE-------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ SKIN & PELAGE RED HAIR COAT HEAD-MAXILLARY-RIGHT LIMBS-ALL LIMBS-FRONT PERINEAL AREA VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-CERVICAL VENTRAL-THORACIC ROUGH HAIR COAT SCALY SKIN TAIL TAIL-DISTAL SORE/SCAB AXILLARY REGION-LEFT AXILLARY REGION-RIGHT DORSAL DORSAL-CERVICAL DORSAL-CERVICAL-LEFT DORSAL-CERVICAL-RIGHT DORSAL-THORACIC-LEFT DORSAL-THORACIC-RIGHT EAR-RIGHT EARS HEAD-ENTIRE 1000000 0100000 1000000 0010000 4245001 0010000 0010010 1010000 3414030 0010000 0000000 1000000 2100000 2311000 1000000 0000000 0010000 0001000 0001000 0010000 0001000 0000011 148 Table 5 Covance 6329-212 3M T-6316.1 Summary of Clinical Observations PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: ---------------MALE-------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ SKIN & PELAGE SORE/SCAB HEAD-CRANIAL HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT LATERAL-ABDOMINAL-LEFT LATERAL-ABDOMINAL-RIGHT LATERAL-CERVICAL-LEFT LATERAL-CERVICAL-RIGHT LIMB-FRONT-LEFT LIMB-HIND-LEFT LIMB-HIND-RIGHT LIMBS-HIND LATERAL-THORACIC-RIGHT LUMBAR-LEFT MOUTH PAW-FRONT-RIGHT PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-FRONT PAWS-HIND SACRAL-LEFT SCROTUM SCROTUM-RIGHT SHOULDER-LEFT SHOULDER-RIGHT 0100000 0001000 0112000 1010000 0100000 1000000 1000000 0000000 0320000 0100000 1000000 0000000 0000000 0001000 0010000 5 3 10 5 0 9 0 6684040 0000000 5355020 0000000 0011000 0010000 0210000 0000000 149 Table 5 Covance 6329-212 3M T-6316.1 Summary of Clinical Observations PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: ---------------MALE-------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ SKIN & PELAGE SORE/SCAB TAIL TAIL-PROXIMAL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL VENTRAL-CERVICAL-LEFT VENTRAL-CERVICAL-RIGHT VENTRAL-THORACIC VENTRAL-THORACIC-RIGHT YELLOW HAIR COAT 2221000 0100000 1123020 0100000 0000000 1200110 0010000 0010000 0100000 0000000 9267081 150 Table 5 Covance 6329-212 3M T-6316.1 Summary of Clinical Observations PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: --------------FEMALE------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ *** TOP OF LIST *** APPEARANCE BENT TAIL 0000010 CONVULSIONS 0012000 DEHYDRATED 0020030 HEAD TILT LEFT 5231020 RIGHT 6651010 HUNCHED 5 4 12 8 0 4 0 LIMITED USE LIMB-HIND-RIGHT 0000000 LIMBS-FRONT 0000000 LIMBS-HIND 0000000 PAW-HIND-LEFT 2000000 PAW-HIND-RIGHT 1000000 PAWS-FRONT 0000000 PAWS-HIND 0000000 MALOCCLUSION 10 19 12 19 0 11 0 MISSING TAIL-PROXIMAL 0000010 TEETH 1100000 OBESE 0000000 PARALYSIS LIMB-FRONT-LEFT 0000000 LIMBS-HIND 0010000 PROLAPSED VAGINA 0000010 151 Table 5 Covance 6329-212 3M T-6316.1 Summary of Clinical Observations PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: --------------FEMALE------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ APPEARANCE PROTRUDING PENIS SWOLLEN DORSAL-CERVICAL INGUINAL REGION-LEFT INGUINAL REGION-RIGHT LIMB-HIND-LEFT LIMB-HIND-RIGHT LIMBS-HIND MOUTH NOSE PAW-FRONT-LEFT PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-FRONT PAWS-HIND TAIL-PROXIMAL VENTRAL-ABDOMINAL VENTRAL-CERVICAL THIN GENITAL DISCHARGE, GREEN 0000000 0000000 1000000 1000000 0100000 0000010 0000000 1100020 0000010 0100000 2011000 1100000 0000000 0000000 0000000 2100020 0000000 17 19 23 22 0 16 0 1000000 BEHAVIOR ATAXIC CIRCLING LEFT 3371030 0120000 152 Table 5 Covance 6329-212 3M T-6316.1 Summary of Clinical Observations PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: --------------FEMALE------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ BEHAVIOR CIRCLING RIGHT HYPOACTIVE RECUMBENT LATERAL STERNAL SENSITIVE TO VOCALIZATION TOUCH 0100000 7 11 14 14 0 9 0 0111010 2210000 3101000 0100000 DISCHARGE GENITAL RED IN MUCOID ORAL RED IN CLEAR NASAL RED IN UNKNOWN RED IN COLOR COLOR COLOR COLOR 3112040 0001000 0112000 0011000 2829050 0000000 EXCRETION DISCOLORED URINE RED IN COLOR ORANGE IN COLOR 0010000 0000000 153 Table 5 Covance 6329-212 3M T-6316.1 Summary of Clinical Observations PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: --------------FEMALE------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ EXCRETION FEW FECES LIQUID FECES NO FECES NONFORMED FECES 18 16 15 16 0 10 0 0000000 6754090 3000020 EYES CLEAR DISCHARGE EYE-LEFT EYE-RIGHT EYES CLOUDY DISCHARGE EYE-RIGHT DISCOLORED/DARK RED EYE-LEFT DILATED PUPIL EYES DISCOLORED/LIGHT RED EYE-LEFT EYE-RIGHT EYES OPAQUE EYE-LEFT EYE-RIGHT EYES 1020000 0000010 0001000 1000000 0010000 0000000 0000000 0001000 0000000 0000000 0000010 0000000 154 Table 5 Covance 6329-212 3M T-6316.1 Summary of Clinical Observations PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: --------------FEMALE------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ EYES PALE EYE-RIGHT EYES RED DISCHARGE EYE-LEFT EYE-RIGHT EYES SQUINTED PERI-ORBITAL PERI-ORBITAL-RIGHT 0000000 3413020 3452040 4222020 11 16 12 7 0 8 0 2232010 0000000 RESPIRATION AUDIBLE IRREGULAR LABORED 0446030 0411010 5552020 SKIN & PELAGE ALOPECIA BROWN HAIR COAT HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT PERINEAL AREA SCROTUM VENTRAL-ABDOMINAL GENERALIZED 0012040 0000000 0000000 1142010 0000000 0423010 0000020 155 Table 5 Covance 6329-212 3M T-6316.1 Summary of Clinical Observations PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: --------------FEMALE------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ SKIN & PELAGE BLUE SKIN PAW-HIND-LEFT VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL BROWN SKIN PAWS-ALL VENTRAL-ABDOMINAL COLD TO TOUCH LIMBS-ALL LIMBS-HIND ENTIRE BODY BLACK SKIN VENTRAL-ABDOMINAL-LEFT VENTRAL-THORACIC-RIGHT PALE EARS ENTIRE BODY RED HAIR COAT DORSAL-CERVICAL DORSAL-CERVICAL-RIGHT HEAD-ENTIRE HEAD-CRANIAL HEAD-MAXILLARY-LEFT 0000000 0010010 1100000 0010000 1000000 0000000 0000000 0010000 0100000 2221020 0010000 0100010 0200000 5432040 0000010 0001000 5 10 6 10 0 4 0 0112020 0000000 156 Table 5 Covance 6329-212 3M T-6316.1 Summary of Clinical Observations PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: --------------FEMALE------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ SKIN & PELAGE RED HAIR COAT HEAD-MAXILLARY-RIGHT LIMBS-ALL LIMBS-FRONT PERINEAL AREA VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-CERVICAL VENTRAL-THORACIC ROUGH HAIR COAT SCALY SKIN TAIL TAIL-DISTAL SORE/SCAB AXILLARY REGION-LEFT AXILLARY REGION-RIGHT DORSAL DORSAL-CERVICAL DORSAL-CERVICAL-LEFT DORSAL-CERVICAL-RIGHT DORSAL-THORACIC-LEFT DORSAL-THORACIC-RIGHT EAR-RIGHT EARS HEAD-ENTIRE 0000000 0000000 1002000 2200020 2216010 0000000 0000000 0001000 0022060 0000010 0001000 1010000 2210000 1112010 0100000 1000000 0000000 0000020 0000000 0000000 0000000 0000010 157 Table 5 Covance 6329-212 3M T-6316.1 Summary of Clinical Observations PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: --------------FEMALE------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ SKIN & PELAGE SORE/SCAB HEAD-CRANIAL HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT LATERAL-ABDOMINAL-LEFT LATERAL-ABDOMINAL-RIGHT LATERAL-CERVICAL-LEFT LATERAL-CERVICAL-RIGHT LIMB-FRONT-LEFT LIMB-HIND-LEFT LIMB-HIND-RIGHT LIMBS-HIND LATERAL-THORACIC-RIGHT LUMBAR-LEFT MOUTH PAW-FRONT-RIGHT PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-FRONT PAWS-HIND SACRAL-LEFT SCROTUM SCROTUM-RIGHT SHOULDER-LEFT SHOULDER-RIGHT 0100000 0100000 0000000 1000000 0010000 0001000 0001000 1000000 1000000 1000000 2000020 0001000 0001000 1202010 0000000 4220020 3112010 0100000 4101030 0001000 0000000 0000000 0000000 0100000 158 Table 5 Covance 6329-212 3M T-6316.1 Summary of Clinical Observations PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: --------------FEMALE------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ SKIN & PELAGE SORE/SCAB TAIL TAIL-PROXIMAL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL VENTRAL-CERVICAL-LEFT VENTRAL-CERVICAL-RIGHT VENTRAL-THORACIC VENTRAL-THORACIC-RIGHT YELLOW HAIR COAT *** END OF LIST *** 0000010 0000000 0010030 0020000 0120030 1102000 0120000 0010000 0000000 0000010 8 7 15 10 0 8 0 159 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: ---------------MALE-------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ *** TOP OF LIST *** MASS(ES) AXILLARY REGION-LEFT WEEK 26 0000000 WEEK 33 0000000 WEEK 43 1000000 WEEK 47 0000000 WEEK 56 0000000 WEEK 58 0000000 WEEK 59 0000000 WEEK 61 1000000 WEEK 63 0000000 WEEK 64 0000000 WEEK 65 0000000 WEEK 68 0000000 WEEK 71 0000000 WEEK 74 0000000 WEEK 75 0000000 WEEK 78 0000000 WEEK 81 0000000 WEEK 82 0000000 WEEK 84 0000000 WEEK 85 0000000 WEEK 86 0000000 WEEK 87 0000000 WEEK 88 0001000 WEEK 89 0100000 160 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: ---------------MALE-------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) AXILLARY REGION-LEFT WEEK 92 WEEK 97 WEEK 99 WEEK 100 WEEK 105 AXILLARY REGION-RIGHT WEEK 31 WEEK 35 WEEK 38 WEEK 45 WEEK 47 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 67 WEEK 68 WEEK 71 WEEK 74 WEEK 76 WEEK 82 WEEK 84 WEEK 85 WEEK 87 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 1000000 0000000 0000000 0000000 2000000 0000000 0000010 161 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: ---------------MALE-------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) AXILLARY REGION-RIGHT WEEK 88 WEEK 89 WEEK 91 WEEK 92 WEEK 93 WEEK 96 WEEK 97 WEEK 99 WEEK 102 WEEK 105 DORSAL WEEK 49 WEEK 56 WEEK 64 WEEK 68 WEEK 76 WEEK 82 WEEK 90 WEEK 98 WEEK 99 WEEK 103 WEEK 105 DORSAL-CERVICAL-LEFT WEEK 80 0000000 0001000 0100000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 1000000 0001000 0010000 0000000 0100000 0000000 0000000 0010000 0000000 0000000 0100000 0000000 162 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: ---------------MALE-------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) DORSAL-CERVICAL-LEFT WEEK 82 HEAD-MAXILLARY-LEFT WEEK 80 WEEK 82 HEAD-MAXILLARY-RIGHT WEEK 17 WEEK 48 WEEK 51 WEEK 61 INGUINAL REGION-LEFT WEEK 64 WEEK 75 WEEK 76 WEEK 80 WEEK 81 WEEK 84 WEEK 86 WEEK 87 WEEK 88 WEEK 89 WEEK 92 WEEK 93 WEEK 94 WEEK 98 0000000 0000000 1000000 0000000 0000000 0100000 0100000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 163 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: ---------------MALE-------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) INGUINAL REGION-LEFT WEEK 105 INGUINAL REGION-RIGHT WEEK 72 WEEK 74 WEEK 75 WEEK 76 WEEK 80 WEEK 83 WEEK 84 WEEK 86 WEEK 87 WEEK 89 WEEK 92 WEEK 93 WEEK 94 WEEK 97 WEEK 98 LIMB-HIND-LEFT WEEK 25 WEEK 76 LIMB-HIND-RIGHT WEEK 71 WEEK 98 0000000 0000000 0000000 0000010 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 1000000 0000000 0000000 0001000 0000000 0100000 164 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: ---------------MALE-------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) MOUTH WEEK 20 WEEK 56 WEEK 67 WEEK 70 WEEK 71 WEEK 82 WEEK 85 WEEK 104 SHOULDER-LEFT WEEK 40 TAIL-PROXIMAL WEEK 86 VENTRAL-ABDOMINAL WEEK 33 WEEK 35 WEEK 40 WEEK 44 WEEK 49 WEEK 51 WEEK 57 WEEK 59 WEEK 62 WEEK 72 WEEK 74 1000000 0000000 0001000 0000000 0000000 0010000 0000000 0000010 0010000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 165 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: ---------------MALE-------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) VENTRAL-ABDOMINAL WEEK 77 WEEK 82 WEEK 83 WEEK 85 WEEK 88 WEEK 90 WEEK 92 WEEK 95 WEEK 102 VENTRAL-ABDOMINAL-LEFT WEEK 21 WEEK 22 WEEK 37 WEEK 45 WEEK 46 WEEK 49 WEEK 51 WEEK 53 WEEK 57 WEEK 61 WEEK 62 WEEK 64 WEEK 67 WEEK 71 0100000 0000000 0010000 0000000 0000000 0000000 0000000 0000000 0001000 0100000 0000000 0001000 0000000 0000000 0000000 0100000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 166 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: ---------------MALE-------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 72 WEEK 74 WEEK 75 WEEK 76 WEEK 82 WEEK 84 WEEK 85 WEEK 86 WEEK 99 WEEK 100 WEEK 105 VENTRAL-ABDOMINAL-RIGHT WEEK 29 WEEK 32 WEEK 36 WEEK 38 WEEK 40 WEEK 49 WEEK 51 WEEK 53 WEEK 56 WEEK 62 WEEK 66 WEEK 70 0000000 0000000 0000000 0010000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0001000 0000000 0000000 0001000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 167 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: ---------------MALE-------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 WEEK 81 WEEK 82 WEEK 84 WEEK 85 WEEK 88 WEEK 91 WEEK 94 WEEK 97 WEEK 98 WEEK 100 WEEK 105 VENTRAL-CERVICAL WEEK 62 WEEK 64 WEEK 68 WEEK 73 WEEK 75 WEEK 80 WEEK 95 WEEK 100 VENTRAL-CERVICAL-LEFT WEEK 43 WEEK 46 0000000 0010000 1000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 1000000 0000000 0000000 0000000 0000000 168 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: ---------------MALE-------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 48 WEEK 61 WEEK 79 WEEK 87 WEEK 88 VENTRAL-CERVICAL-RIGHT WEEK 43 WEEK 61 WEEK 78 WEEK 105 VENTRAL-THORACIC WEEK 42 WEEK 54 WEEK 81 VENTRAL-THORACIC-LEFT WEEK 67 WEEK 79 WEEK 95 WEEK 101 VENTRAL-THORACIC-RIGHT WEEK 75 WEEK 84 HEAD WEEK 82 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 1000000 0000000 0000000 0000000 0000000 0001000 0000000 0000000 0000000 0000000 169 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: ---------------MALE-------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) HEAD WEEK 86 WEEK 102 0000000 0000010 WART LIKE LESION(S) DORSAL WEEK 47 WEEK 51 WEEK 61 WEEK 69 WEEK 76 WEEK 79 WEEK 89 WEEK 95 WEEK 97 WEEK 105 DORSAL-CERVICAL-LEFT WEEK 105 EAR-LEFT WEEK 47 WEEK 65 WEEK 93 HEAD-MAXILLARY-LEFT WEEK 76 WEEK 89 0100000 1000000 1000000 0010000 0100000 0010000 0000000 0000010 0100000 2000000 0010000 0100000 0000010 1000000 0100000 0000000 170 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: ---------------MALE-------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ WART LIKE LESION(S) HEAD-MAXILLARY-RIGHT WEEK 67 MOUTH WEEK 21 WEEK 23 WEEK 31 WEEK 52 WEEK 83 WEEK 87 WEEK 91 WEEK 95 NOSE WEEK 79 PAW-FRONT-LEFT WEEK 95 PAW-FRONT-RIGHT WEEK 95 PAW-HIND-LEFT WEEK 89 PAW-HIND-RIGHT WEEK 34 SHOULDER-LEFT WEEK 37 TAIL WEEK 19 0000000 0000000 0000000 0000000 0000000 0000010 0100000 0100000 0001000 0000010 1000000 1000000 0010000 0100000 0010000 0100000 171 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: ---------------MALE-------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ WART LIKE LESION(S) TAIL WEEK 22 WEEK 82 WEEK 93 WEEK 104 TAIL-PROXIMAL WEEK 88 VENTRAL-ABDOMINAL WEEK 64 WEEK 69 WEEK 76 WEEK 85 VENTRAL-ABDOMINAL-LEFT WEEK 79 VENTRAL-THORACIC WEEK 46 VENTRAL-THORACIC-RIGHT WEEK 69 WEEK 84 WEEK 87 0120000 1000000 0100000 0100000 0000000 0000010 0100000 0000010 0100000 0100000 0000000 1000000 0100000 0100000 172 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: --------------FEMALE------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ *** TOP OF LIST *** MASS(ES) AXILLARY REGION-LEFT WEEK 26 1000000 WEEK 33 0010000 WEEK 43 0010000 WEEK 47 0100000 WEEK 56 1000000 WEEK 58 1010000 WEEK 59 1100000 WEEK 61 0000000 WEEK 63 1001000 WEEK 64 0010000 WEEK 65 1000000 WEEK 68 0200000 WEEK 71 1010010 WEEK 74 2011000 WEEK 75 1010000 WEEK 78 0000010 WEEK 81 0010000 WEEK 82 1010000 WEEK 84 0010000 WEEK 85 1101000 WEEK 86 0001000 WEEK 87 0010010 WEEK 88 0000000 WEEK 89 0000000 173 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: --------------FEMALE------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) AXILLARY REGION-LEFT WEEK 92 WEEK 97 WEEK 99 WEEK 100 WEEK 105 AXILLARY REGION-RIGHT WEEK 31 WEEK 35 WEEK 38 WEEK 45 WEEK 47 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 67 WEEK 68 WEEK 71 WEEK 74 WEEK 76 WEEK 82 WEEK 84 WEEK 85 WEEK 87 0101000 1010000 1000000 0001000 0010000 0010000 0100000 2200000 0001000 1000000 0001000 0021000 1000000 1100000 1000000 1000010 1100000 0200000 1100000 0110000 0101000 1021000 1110000 174 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: --------------FEMALE------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) AXILLARY REGION-RIGHT WEEK 88 WEEK 89 WEEK 91 WEEK 92 WEEK 93 WEEK 96 WEEK 97 WEEK 99 WEEK 102 WEEK 105 DORSAL WEEK 49 WEEK 56 WEEK 64 WEEK 68 WEEK 76 WEEK 82 WEEK 90 WEEK 98 WEEK 99 WEEK 103 WEEK 105 DORSAL-CERVICAL-LEFT WEEK 80 1000000 0011000 0000000 0000010 0120000 0010000 0010000 0010000 1000000 0010000 0000000 0000000 0000000 0001000 0000000 1000000 0011000 0000000 0001000 0001000 0000000 0001000 175 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: --------------FEMALE------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) DORSAL-CERVICAL-LEFT WEEK 82 HEAD-MAXILLARY-LEFT WEEK 80 WEEK 82 HEAD-MAXILLARY-RIGHT WEEK 17 WEEK 48 WEEK 51 WEEK 61 INGUINAL REGION-LEFT WEEK 64 WEEK 75 WEEK 76 WEEK 80 WEEK 81 WEEK 84 WEEK 86 WEEK 87 WEEK 88 WEEK 89 WEEK 92 WEEK 93 WEEK 94 WEEK 98 0010000 0010000 0000000 0001000 0001000 0000000 0000000 1200000 0110000 1010000 0011000 0010000 0210000 0200000 1100010 0010000 0100000 0000010 0010000 0100000 0000010 176 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: --------------FEMALE------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) INGUINAL REGION-LEFT WEEK 105 INGUINAL REGION-RIGHT WEEK 72 WEEK 74 WEEK 75 WEEK 76 WEEK 80 WEEK 83 WEEK 84 WEEK 86 WEEK 87 WEEK 89 WEEK 92 WEEK 93 WEEK 94 WEEK 97 WEEK 98 LIMB-HIND-LEFT WEEK 25 WEEK 76 LIMB-HIND-RIGHT WEEK 71 WEEK 98 0011000 1100000 0100000 0000000 1100000 0100000 0100000 0200010 0010000 1100000 0010000 0010000 0100000 1000000 0100000 1100000 1000000 0000000 1000000 0000000 177 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: --------------FEMALE------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) MOUTH WEEK 20 WEEK 56 WEEK 67 WEEK 70 WEEK 71 WEEK 82 WEEK 85 WEEK 104 SHOULDER-LEFT WEEK 40 TAIL-PROXIMAL WEEK 86 VENTRAL-ABDOMINAL WEEK 33 WEEK 35 WEEK 40 WEEK 44 WEEK 49 WEEK 51 WEEK 57 WEEK 59 WEEK 62 WEEK 72 WEEK 74 0000000 0000010 0000000 0001000 1000000 0000000 0100000 0000000 0000000 0010000 0001000 0200010 0100000 0000010 0100000 1100000 0010000 0000010 0000010 0010000 0000010 178 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 20 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: --------------FEMALE------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) VENTRAL-ABDOMINAL WEEK 77 WEEK 82 WEEK 83 WEEK 85 WEEK 88 WEEK 90 WEEK 92 WEEK 95 WEEK 102 VENTRAL-ABDOMINAL-LEFT WEEK 21 WEEK 22 WEEK 37 WEEK 45 WEEK 46 WEEK 49 WEEK 51 WEEK 53 WEEK 57 WEEK 61 WEEK 62 WEEK 64 WEEK 67 WEEK 71 0000000 0100000 0000000 0001000 0100000 1010000 0100000 1000000 0000010 0000000 0010000 0000000 1000000 0100010 0100000 0000010 0010000 0010000 0000010 0010000 0100000 0001010 0111000 179 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 21 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: --------------FEMALE------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 72 WEEK 74 WEEK 75 WEEK 76 WEEK 82 WEEK 84 WEEK 85 WEEK 86 WEEK 99 WEEK 100 WEEK 105 VENTRAL-ABDOMINAL-RIGHT WEEK 29 WEEK 32 WEEK 36 WEEK 38 WEEK 40 WEEK 49 WEEK 51 WEEK 53 WEEK 56 WEEK 62 WEEK 66 WEEK 70 0010000 0001000 0100000 0000000 0200000 0002000 0001000 1000000 0010000 0010000 0010000 0000000 1000000 1000000 0010000 0010000 0200000 1011010 0010000 0100000 0020000 0000010 0100000 180 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 22 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: --------------FEMALE------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 WEEK 81 WEEK 82 WEEK 84 WEEK 85 WEEK 88 WEEK 91 WEEK 94 WEEK 97 WEEK 98 WEEK 100 WEEK 105 VENTRAL-CERVICAL WEEK 62 WEEK 64 WEEK 68 WEEK 73 WEEK 75 WEEK 80 WEEK 95 WEEK 100 VENTRAL-CERVICAL-LEFT WEEK 43 WEEK 46 0000010 0000000 0010000 0000010 0001000 0001000 1000000 0001000 0101000 1000000 0001000 0100000 1010000 0100000 0010000 0001000 0001000 0000000 0100000 1000000 0000010 0010000 181 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 23 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: --------------FEMALE------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 48 WEEK 61 WEEK 79 WEEK 87 WEEK 88 VENTRAL-CERVICAL-RIGHT WEEK 43 WEEK 61 WEEK 78 WEEK 105 VENTRAL-THORACIC WEEK 42 WEEK 54 WEEK 81 VENTRAL-THORACIC-LEFT WEEK 67 WEEK 79 WEEK 95 WEEK 101 VENTRAL-THORACIC-RIGHT WEEK 75 WEEK 84 HEAD WEEK 82 0000010 0001000 0000010 0000010 1000000 0001000 0001000 0100000 0010000 0000000 0001000 0010000 0002000 0010000 0000000 1000000 0000010 0100000 0100000 182 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 24 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: --------------FEMALE------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) HEAD WEEK 86 WEEK 102 1000000 0000000 WART LIKE LESION(S) DORSAL WEEK 47 WEEK 51 WEEK 61 WEEK 69 WEEK 76 WEEK 79 WEEK 89 WEEK 95 WEEK 97 WEEK 105 DORSAL-CERVICAL-LEFT WEEK 105 EAR-LEFT WEEK 47 WEEK 65 WEEK 93 HEAD-MAXILLARY-LEFT WEEK 76 WEEK 89 0000000 0000000 0000000 0000000 0000000 0000000 0001000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 1000000 183 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 25 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: --------------FEMALE------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ WART LIKE LESION(S) HEAD-MAXILLARY-RIGHT WEEK 67 MOUTH WEEK 21 WEEK 23 WEEK 31 WEEK 52 WEEK 83 WEEK 87 WEEK 91 WEEK 95 NOSE WEEK 79 PAW-FRONT-LEFT WEEK 95 PAW-FRONT-RIGHT WEEK 95 PAW-HIND-LEFT WEEK 89 PAW-HIND-RIGHT WEEK 34 SHOULDER-LEFT WEEK 37 TAIL WEEK 19 0100000 1000000 0001000 0001000 0100000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 184 Table 6 Covance 6329-212 3M T-6316.1 Summary of Mass Observations PAGE: 26 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-732 SEX: --------------FEMALE------------- GROUP: 1 2 3 4 5 6 7 CATEGORY DOSE: 0 3 30 100 300 100 300 KEYWORD QUALIFIER NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------------------------------------------------------------------ WART LIKE LESION(S) TAIL WEEK 22 WEEK 82 WEEK 93 WEEK 104 TAIL-PROXIMAL WEEK 88 VENTRAL-ABDOMINAL WEEK 64 WEEK 69 WEEK 76 WEEK 85 VENTRAL-ABDOMINAL-LEFT WEEK 79 VENTRAL-THORACIC WEEK 46 VENTRAL-THORACIC-RIGHT WEEK 69 WEEK 84 WEEK 87 *** END OF LIST *** 0000000 0000000 0000000 0000000 0001000 0000000 0000000 0000000 0000000 0000000 0010000 0000000 0000000 0000000 185 Table 7 Covance 6329-212 3M T-6316.1 Summary of Body Weight Data (g) PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------------MALE--------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 1 N 70 MEAN 261 S.D. 20.4 60 260 19.9 60 260 20.5 70 258 19.2 69 257 19.0 40 254 17.8 40 256 19.2 2 N 70 MEAN 308 S.D. 23.1 60 307 22.5 60 302 24.5 70 298 * 21.7 70 288 * 23.0 40 296 * 20.0 40 286 * 21.2 3 N 70 MEAN 350 S.D. 25.7 60 351 26.6 60 344 29.9 70 337 * 25.3 70 318 * 25.9 40 334 * 24.2 40 313 * 24.0 4 N 70 MEAN 387 S.D. 29.0 60 389 29.9 60 379 34.8 70 367 * 28.3 70 338 * 28.8 40 364 * 27.8 40 334 * 27.3 5 N 65 MEAN 417 S.D. 31.5 55 416 31.5 55 404 38.2 65 393 * 30.5 64 355 * 31.8 40 391 * 32.5 40 351 * 30.3 6 N 65 MEAN 444 S.D. 33.6 55 444 33.9 55 431 40.5 65 418 * 33.6 65 370 * 35.3 40 416 * 36.2 40 366 * 33.1 7 N 65 MEAN 467 S.D. 37.0 55 467 37.1 55 450 42.6 65 436 * 35.5 65 380 * 37.6 40 427 * 42.8 39 374 * 33.9 186 Table 7 Covance 6329-212 3M T-6316.1 Summary of Body Weight Data (g) PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------------MALE--------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 8 N 65 MEAN 483 S.D. 39.5 55 480 40.0 55 464 * 43.4 65 449 * 39.4 63 386 * 42.4 39 448 * 44.8 39 384 * 38.5 9 N 60 54 55 65 0 39 0 MEAN 504 507 483 * 465 * 464 * S.D. 43.4 40.2 45.9 40.9 51.0 10 N 60 54 55 65 0 39 0 MEAN 521 524 497 * 477 * 473 * S.D. 45.1 41.1 49.3 41.8 57.6 11 N 60 54 55 65 0 39 0 MEAN 532 537 510 * 486 * 484 * S.D. 45.4 43.3 49.8 43.7 62.4 12 N 60 54 55 65 0 39 0 MEAN 543 550 522 496 * 496 * S.D. 46.8 44.6 51.5 46.9 64.9 13 N 60 54 55 65 0 38 0 MEAN 553 559 531 504 * 515 * S.D. 47.7 48.3 52.7 51.2 44.9 14 N 60 54 55 64 0 38 0 MEAN 563 567 538 * 513 * 522 * S.D. 50.1 48.0 54.5 45.3 45.5 187 Table 7 Covance 6329-212 3M T-6316.1 Summary of Body Weight Data (g) PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------------MALE--------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 15 N 55 49 50 59 0 38 0 MEAN 569 574 540 * 519 * 528 * S.D. 51.8 50.9 51.9 45.8 47.5 16 N 55 49 50 59 0 38 0 MEAN 578 585 547 * 528 * 536 * S.D. 52.9 52.3 53.1 45.6 48.4 17 N 55 49 50 59 0 38 0 MEAN 590 595 557 * 536 * 546 * S.D. 55.0 54.0 53.4 46.4 50.2 21 N 55 49 50 59 0 38 0 MEAN 619 625 582 * 555 * 567 * S.D. 58.8 58.3 57.0 51.4 55.5 25 N 55 49 50 59 0 38 0 MEAN 644 651 603 * 572 * 582 * S.D. 62.8 64.2 62.1 51.3 60.7 29 N 55 49 50 59 0 38 0 MEAN 663 668 618 * 590 * 602 * S.D. 67.1 69.6 66.5 54.7 64.8 33 N 55 49 50 59 0 38 0 MEAN 678 682 634 * 599 * 612 * S.D. 71.5 72.0 70.2 57.5 64.5 188 Table 7 Covance 6329-212 3M T-6316.1 Summary of Body Weight Data (g) PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------------MALE--------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 37 N 55 48 50 59 0 38 0 MEAN 694 701 646 * 610 * 623 * S.D. 77.1 75.1 74.0 57.6 67.4 41 N 55 48 50 59 0 38 0 MEAN 700 714 653 * 619 * 632 * S.D. 71.4 75.2 77.1 60.1 68.2 45 N 54 48 50 58 0 37 0 MEAN 723 740 678 * 639 * 650 * S.D. 76.2 82.8 82.6 64.9 76.4 49 N 54 48 50 58 0 37 0 MEAN 741 758 698 * 654 * 666 * S.D. 78.3 91.5 87.5 68.7 80.4 53 N 54 48 50 58 0 36 0 MEAN 762 777 716 * 672 * 688 * S.D. 81.9 95.0 92.5 68.4 86.3 57 N 49 48 48 48 0 36 0 MEAN 769 777 726 683 * 707 * S.D. 87.5 118.4 87.5 68.7 93.6 61 N 49 45 48 47 0 36 0 MEAN 781 802 739 689 * 721 * S.D. 92.9 121.7 96.7 76.1 114.9 189 Table 7 Covance 6329-212 3M T-6316.1 Summary of Body Weight Data (g) PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------------MALE--------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 65 N 46 44 47 45 0 34 0 MEAN 787 815 742 695 * 747 S.D. 98.7 117.5 104.0 72.9 95.5 69 N 44 43 46 44 0 33 0 MEAN 789 813 737 685 * 767 S.D. 104.8 129.4 112.6 93.7 103.5 73 N 40 41 41 41 0 33 0 MEAN 811 829 757 707 * 784 S.D. 98.5 128.1 99.1 95.7 115.4 77 N 40 40 38 38 0 32 0 MEAN 799 827 746 719 * 789 S.D. 123.4 128.2 114.3 93.0 127.8 81 N 36 37 34 36 0 30 0 MEAN 799 820 753 725 * 780 S.D. 120.9 137.6 121.4 88.7 144.0 85 N 35 33 32 35 0 24 0 MEAN 791 837 772 721 * 754 S.D. 134.2 130.2 95.1 90.5 151.5 89 N 30 32 32 31 0 21 0 MEAN 791 840 763 724 760 S.D. 134.6 134.2 102.7 97.4 154.9 190 Table 7 Covance 6329-212 3M T-6316.1 Summary of Body Weight Data (g) PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------------MALE--------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 93 N 27 30 29 29 0 19 0 MEAN 763 828 778 735 758 S.D. 165.6 145.8 109.4 93.7 170.7 97 N 21 27 27 26 0 16 0 MEAN 791 852 757 724 788 S.D. 168.3 136.8 132.1 93.4 160.6 101 N 15 23 24 23 0 12 0 MEAN 805 821 764 698 802 S.D. 161.0 145.5 135.9 99.5 157.7 105 N 14 22 22 17 0 10 0 MEAN 795 781 766 688 806 S.D. 185.0 173.4 138.2 123.6 179.0 191 Table 7 Covance 6329-212 3M T-6316.1 Summary of Body Weight Data (g) PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------------FEMALE-------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 1 N 70 MEAN 165 S.D. 13.2 60 163 13.3 60 165 12.9 70 164 12.1 70 164 12.6 39 164 12.5 40 163 12.0 2 N 70 MEAN 188 S.D. 16.9 60 185 14.7 60 185 14.4 70 181 * 13.5 70 172 * 13.3 40 181 14.1 40 175 * 11.7 3 N 70 MEAN 211 S.D. 20.8 60 205 18.5 60 205 16.5 70 200 * 15.5 70 182 * 15.1 40 202 15.8 40 186 * 12.2 4 N 70 MEAN 229 S.D. 22.3 60 224 20.2 60 222 18.3 70 215 * 17.1 70 191 * 15.2 40 214 * 15.9 40 196 * 12.7 5 N 65 MEAN 244 S.D. 25.4 55 236 21.2 55 233 * 19.9 65 225 * 18.6 65 196 * 16.4 40 223 * 16.7 40 200 * 13.3 6 N 65 MEAN 258 S.D. 28.3 55 246 22.7 55 245 21.2 65 235 * 19.4 65 202 * 16.6 40 234 * 17.3 40 206 * 14.4 7 N 65 MEAN 267 S.D. 30.0 55 256 23.8 55 253 22.7 65 239 * 19.6 65 205 * 16.5 40 240 * 17.4 40 210 * 13.2 192 Table 7 Covance 6329-212 3M T-6316.1 Summary of Body Weight Data (g) PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------------FEMALE-------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 8 N 65 MEAN 274 S.D. 30.4 55 261 24.9 55 258 * 23.6 65 246 * 20.7 65 212 * 17.1 40 243 * 19.4 40 213 * 14.9 9 N 60 55 55 65 0 40 0 MEAN 283 271 268 * 250 * 250 * S.D. 32.1 25.6 24.1 20.7 18.9 10 N 60 55 55 65 0 40 0 MEAN 290 277 273 * 254 * 253 * S.D. 33.6 27.3 25.1 20.3 21.5 11 N 60 55 55 65 0 40 0 MEAN 295 281 275 * 256 * 255 * S.D. 35.1 28.7 26.3 21.8 19.9 12 N 60 55 55 65 0 40 0 MEAN 302 284 280 * 258 * 259 * S.D. 36.6 28.6 26.7 21.4 20.8 13 N 60 55 55 65 0 40 0 MEAN 306 289 284 * 260 * 261 * S.D. 36.3 27.5 26.6 21.7 20.2 14 N 60 55 55 65 0 40 0 MEAN 308 292 * 286 * 262 * 264 * S.D. 36.3 27.9 27.7 22.3 20.8 193 Table 7 Covance 6329-212 3M T-6316.1 Summary of Body Weight Data (g) PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------------FEMALE-------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 15 N 55 50 50 60 0 40 0 MEAN 310 295 * 288 * 263 * 266 * S.D. 36.0 29.0 27.9 23.3 21.9 16 N 55 50 50 59 0 40 0 MEAN 316 300 * 293 * 266 * 269 * S.D. 36.8 31.0 28.2 23.0 21.7 17 N 55 50 50 59 0 40 0 MEAN 320 306 297 * 270 * 272 * S.D. 37.9 30.2 29.3 23.2 22.2 21 N 55 50 50 59 0 40 0 MEAN 334 318 311 * 277 * 279 * S.D. 41.9 33.7 32.8 24.8 23.3 25 N 55 50 50 58 0 40 0 MEAN 348 334 324 * 286 * 291 * S.D. 47.6 38.7 37.2 26.4 24.5 29 N 55 50 50 58 0 40 0 MEAN 361 346 336 * 294 * 299 * S.D. 51.9 42.7 40.2 28.2 26.6 33 N 55 50 50 58 0 40 0 MEAN 375 359 349 301 * 307 * S.D. 55.1 48.3 44.1 31.8 30.0 194 Table 7 Covance 6329-212 3M T-6316.1 Summary of Body Weight Data (g) PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------------FEMALE-------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 37 N 55 50 50 58 0 40 0 MEAN 384 366 355 * 304 * 313 * S.D. 58.2 48.9 46.4 34.0 32.2 41 N 55 50 50 58 0 40 0 MEAN 400 380 370 313 * 323 * S.D. 64.7 52.3 48.2 36.5 34.8 45 N 55 50 50 58 0 40 0 MEAN 423 397 388 320 * 332 * S.D. 72.3 59.3 54.0 40.5 38.0 49 N 54 50 50 58 0 40 0 MEAN 439 412 396 * 329 * 339 * S.D. 76.0 63.2 55.3 43.1 40.6 53 N 54 50 50 58 0 40 0 MEAN 454 425 404 * 338 * 350 * S.D. 82.1 67.9 62.5 48.7 44.3 57 N 49 49 49 48 0 40 0 MEAN 465 435 411 * 348 * 368 * S.D. 93.3 69.0 66.3 52.0 49.3 61 N 48 48 47 47 0 40 0 MEAN 473 450 426 * 359 * 402 * S.D. 98.7 73.5 70.5 58.1 55.3 195 Table 7 Covance 6329-212 3M T-6316.1 Summary of Body Weight Data (g) PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------------FEMALE-------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 65 N 45 47 46 45 0 37 0 MEAN 483 455 430 * 368 * 424 * S.D. 101.3 75.9 75.7 61.2 57.3 69 N 44 42 44 45 0 37 0 MEAN 492 470 434 * 371 * 433 * S.D. 100.2 78.5 85.2 65.9 73.9 73 N 44 42 38 41 0 34 0 MEAN 487 480 451 381 * 459 S.D. 113.9 79.1 92.7 67.7 70.7 77 N 36 35 34 38 0 31 0 MEAN 500 486 469 377 * 474 S.D. 116.1 87.4 85.8 71.0 65.8 81 N 32 33 34 35 0 29 0 MEAN 513 502 469 381 * 480 S.D. 115.0 85.4 93.6 77.1 80.8 85 N 30 31 30 34 0 26 0 MEAN 506 501 483 382 * 473 S.D. 110.8 97.5 94.6 75.7 91.2 89 N 27 26 27 29 0 22 0 MEAN 496 504 484 387 * 480 S.D. 115.1 96.9 94.3 72.9 115.1 196 Table 7 Covance 6329-212 3M T-6316.1 Summary of Body Weight Data (g) PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------------FEMALE-------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 93 N 22 24 25 29 0 20 0 MEAN 515 495 496 390 * 502 S.D. 117.2 94.6 103.3 76.0 106.0 97 N 17 21 22 27 0 15 0 MEAN 513 488 515 375 * 527 S.D. 105.9 94.3 88.4 87.4 102.2 101 N 15 14 17 23 0 12 0 MEAN 515 511 535 385 * 519 S.D. 110.1 98.2 104.1 86.0 89.4 105 N 13 13 15 19 0 12 0 MEAN 492 526 534 403 522 S.D. 113.3 94.0 114.9 94.5 96.3 197 Table 8 Covance 6329-212 3M T-6316.1 Summary of Body Weight Change Data (g) PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------------MALE--------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 1-15 N 55 49 50 59 0 38 0 MEAN 306 314 281 * 261 * 273 * S.D. 39.7 38.5 37.6 33.8 38.8 15-29 N 55 49 50 59 0 MEAN 94 94 78 * 71 * S.D. 23.8 26.2 23.9 17.0 38 0 74 * 26.4 29-57 N 49 48 48 48 0 36 0 MEAN 110 106 109 92 110 S.D. 35.4 70.9 44.1 38.2 45.2 57-105 N 14 22 22 17 0 10 0 MEAN 48 -11 69 30 122 S.D. 137.3 144.4 89.8 131.5 109.8 1-105 N 14 22 22 17 0 MEAN 540 524 512 434 S.D. 185.4 172.2 132.9 125.5 10 0 551 168.2 198 Table 8 Covance 6329-212 3M T-6316.1 Summary of Body Weight Change Data (g) PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------------FEMALE-------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 1-15 N 55 50 50 60 0 39 0 MEAN 145 131 124 * 97 * 101 * S.D. 29.9 23.8 23.9 19.8 17.0 15-29 N 55 50 50 58 0 MEAN 51 51 48 31 * S.D. 21.6 19.5 19.2 12.9 40 0 34 * 13.1 29-57 N 49 49 49 48 0 MEAN 103 88 76 * 54 * S.D. 47.6 36.1 42.9 31.1 40 0 69 * 27.8 57-105 N 13 13 15 19 0 12 0 MEAN 91 117 105 78 157 S.D. 102.8 58.3 74.7 79.6 89.3 1-105 N 13 13 15 19 0 MEAN 328 363 370 237 S.D. 111.6 93.2 115.5 90.4 11 0 359 97.8 199 Table 9 Covance 6329-212 3M T-6316.1 Summary of Food Consumption Data (g) PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------------MALE--------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 1 N 68 MEAN 175 S.D. 15.2 59 171 15.2 58 172 16.6 69 170 14.9 68 162 * 15.9 39 170 11.3 38 160 * 13.3 2 N 69 MEAN 180 S.D. 13.3 59 177 15.2 57 174 17.5 68 168 * 14.8 68 154 * 14.3 39 170 * 14.9 39 151 * 13.2 3 N 65 MEAN 186 S.D. 13.5 58 181 16.2 58 176 * 17.6 67 169 * 14.0 67 150 * 14.4 40 171 * 14.1 38 147 * 13.0 4 N 65 MEAN 191 S.D. 15.2 54 185 15.7 50 177 * 14.2 63 170 * 13.9 63 147 * 14.6 40 173 * 16.6 40 146 * 17.5 5 N 64 MEAN 190 S.D. 15.6 52 188 15.5 54 180 * 16.8 65 172 * 14.3 60 147 * 16.2 39 173 * 16.0 39 145 * 13.9 6 N 62 MEAN 191 S.D. 16.9 54 188 15.4 52 179 * 15.9 64 170 * 13.2 62 146 * 15.8 40 170 * 21.1 39 146 * 15.9 7 N 58 MEAN 192 S.D. 17.6 53 190 17.0 51 180 * 14.4 64 168 * 18.9 59 145 * 15.9 39 170 * 19.5 38 140 * 24.0 200 Table 9 Covance 6329-212 3M T-6316.1 Summary of Food Consumption Data (g) PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------------MALE--------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 8 N 55 52 53 65 0 38 0 MEAN 191 189 179 * 170 * 171 * S.D. 18.4 15.2 15.1 14.6 25.4 9 N 56 52 52 64 0 39 0 MEAN 187 188 178 * 166 * 167 * S.D. 16.5 16.4 17.7 13.5 27.8 10 N 60 54 55 65 0 39 0 MEAN 186 188 178 164 * 166 * S.D. 15.4 18.2 16.8 16.9 26.1 11 N 58 54 52 64 0 39 0 MEAN 184 187 177 164 * 169 * S.D. 16.8 16.3 15.5 17.9 25.2 12 N 56 53 55 64 0 38 0 MEAN 181 183 174 162 * 171 * S.D. 17.2 15.8 16.2 19.9 15.2 13 N 58 52 54 63 0 37 0 MEAN 188 186 178 * 165 * 174 * S.D. 20.7 15.6 16.0 18.4 14.3 14 N 52 48 48 59 0 38 0 MEAN 185 184 169 * 168 * 173 * S.D. 17.9 17.6 21.5 15.7 14.6 201 Table 9 Covance 6329-212 3M T-6316.1 Summary of Food Consumption Data (g) PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------------MALE--------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 15 N 54 49 46 57 0 38 0 MEAN 191 189 176 * 170 * 175 * S.D. 19.1 17.9 16.9 11.5 15.5 16 N 51 49 49 59 0 38 0 MEAN 187 188 178 * 171 * 176 * S.D. 13.1 18.4 17.4 13.1 15.3 20 N 54 49 48 57 0 38 0 MEAN 192 194 184 177 * 181 * S.D. 16.7 17.8 15.9 19.2 17.0 24 N 55 49 50 58 0 38 0 MEAN 190 189 183 177 * 180 * S.D. 18.2 17.3 16.5 14.2 17.7 28 N 54 49 49 59 0 37 0 MEAN 189 176 * 176 * 176 * 178 * S.D. 17.5 24.2 18.1 14.7 16.2 32 N 55 48 50 57 0 35 0 MEAN 193 190 187 181 * 181 * S.D. 17.5 28.1 15.8 14.7 18.1 36 N 55 47 49 59 0 38 0 MEAN 195 197 188 185 * 187 S.D. 20.7 19.0 16.5 17.0 18.6 202 Table 9 Covance 6329-212 3M T-6316.1 Summary of Food Consumption Data (g) PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------------MALE--------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 40 N 54 46 50 59 0 37 0 MEAN 204 209 203 195 192 * S.D. 26.8 16.4 19.7 17.8 30.6 44 N 54 48 50 57 0 37 0 MEAN 195 196 188 183 * 184 * S.D. 20.1 17.3 18.1 18.3 18.9 48 N 54 48 50 58 0 37 0 MEAN 194 196 186 182 * 185 S.D. 17.6 18.9 18.0 16.5 18.0 52 N 54 48 49 58 0 36 0 MEAN 192 194 186 180 * 183 S.D. 17.4 24.0 17.4 17.0 18.4 56 N 49 48 48 48 0 36 0 MEAN 187 180 179 180 182 S.D. 18.9 43.8 28.0 23.4 25.8 60 N 48 45 48 47 0 36 0 MEAN 189 193 184 174 182 S.D. 22.1 34.1 28.5 24.1 39.9 64 N 46 43 45 44 0 34 0 MEAN 180 189 175 169 183 S.D. 32.1 31.8 29.7 27.7 30.3 203 Table 9 Covance 6329-212 3M T-6316.1 Summary of Food Consumption Data (g) PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------------MALE--------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 68 N 44 42 45 43 0 33 0 MEAN 182 191 172 164 192 S.D. 41.1 36.2 42.6 46.8 20.6 72 N 40 41 40 39 0 33 0 MEAN 195 202 183 185 195 S.D. 23.5 33.9 35.2 28.7 26.5 76 N 40 39 38 37 0 32 0 MEAN 180 184 177 179 187 S.D. 45.4 42.4 43.8 34.4 31.2 80 N 35 33 33 32 0 30 0 MEAN 194 184 181 184 184 S.D. 22.4 54.7 45.5 28.4 46.3 84 N 32 29 32 33 0 24 0 MEAN 184 203 189 170 178 S.D. 32.3 47.6 26.5 44.6 40.3 88 N 30 28 32 31 0 21 0 MEAN 171 190 170 166 169 S.D. 38.0 29.3 26.7 28.6 57.7 92 N 25 27 29 29 0 17 0 MEAN 155 179 172 164 160 S.D. 58.4 32.4 28.9 35.5 40.4 204 Table 9 Covance 6329-212 3M T-6316.1 Summary of Food Consumption Data (g) PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------------MALE--------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 96 N 20 25 25 24 0 14 0 MEAN 159 180 154 154 157 S.D. 47.2 28.7 43.6 31.3 42.1 100 N 14 22 24 21 0 9 0 MEAN 166 162 160 154 168 S.D. 41.4 45.5 28.4 25.0 31.3 104 N 10 19 22 14 0 8 0 MEAN 177 149 155 163 165 S.D. 17.1 43.3 30.7 14.9 17.9 205 Table 9 Covance 6329-212 3M T-6316.1 Summary of Food Consumption Data (g) PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------------FEMALE-------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 1 N 63 MEAN 130 S.D. 23.8 56 124 15.2 54 125 16.0 59 122 * 13.2 57 112 * 14.9 36 121 * 16.0 33 115 * 14.3 2 N 61 MEAN 132 S.D. 20.7 55 130 15.9 50 127 16.6 60 125 16.9 63 105 * 15.9 39 122 * 17.8 35 111 * 16.5 3 N 57 MEAN 136 S.D. 15.5 53 135 22.4 49 129 16.7 57 124 * 14.9 62 99 * 14.3 40 122 * 14.8 36 101 * 12.3 4 N 55 MEAN 141 S.D. 15.8 42 138 23.0 46 134 15.2 57 127 * 15.0 55 96 * 12.5 35 121 * 13.2 36 100 * 13.1 5 N 55 MEAN 142 S.D. 18.7 46 134 15.5 50 136 20.3 56 122 * 12.0 59 97 * 16.0 40 122 * 13.8 35 98 * 10.3 6 N 55 MEAN 138 S.D. 15.8 42 138 18.7 45 135 19.6 59 121 * 11.5 58 96 * 12.6 39 117 * 13.4 36 98 * 14.6 7 N 53 MEAN 140 S.D. 17.6 44 133 14.9 50 130 * 16.9 61 117 * 10.8 60 95 * 11.2 40 116 * 16.2 38 98 * 16.0 206 Table 9 Covance 6329-212 3M T-6316.1 Summary of Food Consumption Data (g) PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------------FEMALE-------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 8 N 52 45 44 57 0 33 0 MEAN 135 140 136 119 * 120 * S.D. 16.3 23.7 25.0 12.0 16.0 9 N 54 47 48 60 0 39 0 MEAN 131 128 127 118 * 114 * S.D. 14.4 19.2 17.3 23.4 13.7 10 N 51 44 47 60 0 37 0 MEAN 135 130 127 115 * 114 * S.D. 21.5 19.6 18.4 15.6 10.4 11 N 52 44 49 62 0 37 0 MEAN 131 128 131 115 * 116 * S.D. 18.9 22.2 25.6 20.1 13.6 12 N 54 43 51 62 0 38 0 MEAN 127 121 128 113 * 114 * S.D. 13.6 13.7 16.5 14.4 16.9 13 N 56 47 54 64 0 39 0 MEAN 124 125 125 113 * 111 * S.D. 15.9 21.8 20.3 20.9 14.0 14 N 52 45 45 57 0 38 0 MEAN 127 126 124 113 * 112 * S.D. 17.8 22.9 15.6 19.5 11.7 207 Table 9 Covance 6329-212 3M T-6316.1 Summary of Food Consumption Data (g) PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------------FEMALE-------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 15 N 48 38 42 55 0 39 0 MEAN 127 125 128 114 * 114 * S.D. 13.4 21.6 16.1 10.8 12.0 16 N 49 42 44 56 0 38 0 MEAN 127 127 127 113 * 114 * S.D. 13.6 16.1 19.4 10.5 13.4 20 N 48 41 47 53 0 38 0 MEAN 134 132 134 120 * 121 * S.D. 15.4 19.6 21.0 12.0 13.1 24 N 53 47 47 54 0 38 0 MEAN 134 140 135 123 * 122 * S.D. 17.1 24.0 20.1 18.1 12.4 28 N 51 46 45 55 0 39 0 MEAN 137 136 133 122 * 125 * S.D. 19.3 19.0 14.6 15.1 13.5 32 N 54 48 48 55 0 36 0 MEAN 140 143 141 130 * 129 * S.D. 20.7 20.6 21.4 18.0 12.0 36 N 54 47 49 54 0 37 0 MEAN 146 149 142 135 * 134 * S.D. 23.1 25.0 19.5 27.0 12.8 208 Table 9 Covance 6329-212 3M T-6316.1 Summary of Food Consumption Data (g) PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------------FEMALE-------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 40 N 51 48 49 56 0 38 0 MEAN 158 162 158 148 149 S.D. 21.8 21.1 22.4 24.9 15.2 44 N 54 48 50 56 0 40 0 MEAN 154 149 150 133 * 137 * S.D. 24.0 20.3 19.6 20.4 20.3 48 N 53 49 50 56 0 39 0 MEAN 147 145 142 137 * 135 * S.D. 22.6 19.3 19.9 26.5 14.8 52 N 54 46 48 55 0 39 0 MEAN 148 144 137 * 133 * 130 * S.D. 24.8 15.7 25.2 20.8 15.6 56 N 48 47 47 47 0 39 0 MEAN 145 138 134 130 * 142 S.D. 29.9 17.8 26.7 23.4 17.5 60 N 46 46 45 44 0 36 0 MEAN 136 144 136 132 148 S.D. 35.9 17.9 21.4 19.8 21.9 64 N 45 46 45 43 0 36 0 MEAN 142 136 136 128 141 S.D. 30.7 34.6 19.1 17.7 20.7 209 Table 9 Covance 6329-212 3M T-6316.1 Summary of Food Consumption Data (g) PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------------FEMALE-------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 68 N 44 40 44 45 0 37 0 MEAN 147 148 141 133 146 S.D. 32.0 19.7 30.9 32.9 36.5 72 N 44 40 38 39 0 34 0 MEAN 139 150 145 134 159 * S.D. 43.0 25.7 23.5 19.9 24.2 76 N 36 33 33 37 0 31 0 MEAN 160 155 146 136 * 153 S.D. 37.8 25.3 26.4 29.5 34.9 80 N 30 28 32 32 0 29 0 MEAN 164 153 147 137 * 154 S.D. 27.2 38.6 30.3 28.6 33.0 84 N 28 28 29 31 0 26 0 MEAN 154 151 143 132 147 S.D. 39.8 33.2 35.6 29.8 30.4 88 N 26 25 26 27 0 20 0 MEAN 143 140 135 125 153 S.D. 35.3 27.0 44.6 23.6 31.5 92 N 20 24 23 29 0 20 0 MEAN 137 127 139 125 133 S.D. 33.8 48.7 27.7 35.3 39.2 210 Table 9 Covance 6329-212 3M T-6316.1 Summary of Food Consumption Data (g) PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------------FEMALE-------------------------------------- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 96 N 16 20 22 24 0 14 0 MEAN 144 126 134 114 * 151 S.D. 33.5 37.8 23.6 39.0 34.8 100 N 14 14 17 23 0 12 0 MEAN 133 128 128 128 144 S.D. 27.2 30.6 26.7 35.9 35.5 104 N 13 13 15 18 0 12 0 MEAN 128 136 127 124 152 S.D. 48.8 10.7 30.7 31.9 26.2 211 Table 10 Covance 6329-212 3M T-6316.1 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------------MALE--------------------------------------- GROUP: 2 3 4 5 6 7 DOSE: 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 1 N 59 58 69 68 39 38 MEAN 0.259 2.618 8.724 25.408 8.873 25.193 S.D. 0.016 0.170 0.561 1.919 0.572 1.230 2 N 59 57 68 68 39 39 MEAN 0.231 2.323 7.544 21.735 7.707 21.759 S.D. 0.012 0.166 0.467 1.585 0.503 1.462 3 N 58 58 67 67 40 38 MEAN 0.210 2.088 6.864 19.533 6.988 19.559 S.D. 0.011 0.123 0.306 1.195 0.339 0.920 4 N 54 50 63 62 40 40 MEAN 0.197 1.953 6.393 18.112 6.533 18.294 S.D. 0.011 0.108 0.280 0.938 0.338 1.663 5 N 52 54 65 59 39 39 MEAN 0.187 1.852 6.067 17.253 6.135 17.415 S.D. 0.009 0.115 0.300 0.987 0.341 0.733 6 N 54 52 64 62 40 39 MEAN 0.177 1.737 5.698 16.576 5.764 16.815 S.D. 0.008 0.102 0.236 0.999 0.435 1.021 7 N 53 51 64 59 39 38 MEAN 0.172 1.694 5.418 16.058 5.536 15.803 S.D. 0.011 0.091 0.409 0.918 0.320 2.209 212 Table 10 Covance 6329-212 3M T-6316.1 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------------MALE--------------------------------------- GROUP: 2 3 4 5 6 7 DOSE: 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 8 N 52 53 65 0 MEAN 0.164 1.629 5.329 S.D. 0.008 0.099 0.251 38 0 5.325 0.496 9 N 52 52 64 0 MEAN 0.156 1.564 5.031 S.D. 0.010 0.104 0.280 39 0 5.043 0.592 10 N 54 55 65 0 MEAN 0.152 1.517 4.871 S.D. 0.011 0.105 0.355 39 0 4.940 0.477 11 N 54 52 64 0 MEAN 0.147 1.472 4.772 S.D. 0.009 0.086 0.324 39 0 4.926 0.349 12 N 53 55 64 0 MEAN 0.141 1.421 4.616 S.D. 0.009 0.101 0.364 38 0 4.800 0.279 13 N 52 54 63 0 MEAN 0.143 1.426 4.616 S.D. 0.010 0.097 0.346 37 0 4.808 0.263 14 N 48 48 59 0 MEAN 0.139 1.357 4.655 S.D. 0.010 0.153 0.348 38 0 4.709 0.241 213 Table 10 Covance 6329-212 3M T-6316.1 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------------MALE--------------------------------------- GROUP: 2 3 4 5 6 7 DOSE: 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 15 N 49 46 57 0 MEAN 0.140 1.397 4.641 S.D. 0.009 0.115 0.222 38 0 4.694 0.241 16 N 49 49 59 0 MEAN 0.137 1.384 4.608 S.D. 0.009 0.105 0.248 38 0 4.661 0.227 20 N 49 48 57 0 MEAN 0.134 1.367 4.545 S.D. 0.008 0.087 0.340 38 0 4.586 0.264 24 N 49 50 58 0 MEAN 0.126 1.309 4.447 S.D. 0.008 0.090 0.291 38 0 4.438 0.245 28 N 49 49 59 0 MEAN 0.114 1.231 4.300 S.D. 0.018 0.116 0.275 37 0 4.249 0.246 32 N 48 50 57 0 MEAN 0.120 1.276 4.298 S.D. 0.016 0.093 0.303 35 0 4.246 0.250 36 N 47 49 59 0 MEAN 0.120 1.261 4.355 S.D. 0.008 0.102 0.428 38 0 4.305 0.255 214 Table 10 Covance 6329-212 3M T-6316.1 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------------MALE--------------------------------------- GROUP: 2 3 4 5 6 7 DOSE: 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 40 N 46 50 59 0 MEAN 0.126 1.340 4.532 S.D. 0.009 0.110 0.407 37 0 4.358 0.631 44 N 48 50 57 0 MEAN 0.115 1.203 4.103 S.D. 0.007 0.094 0.346 37 0 4.069 0.229 48 N 48 50 58 0 MEAN 0.111 1.155 3.994 S.D. 0.010 0.095 0.321 37 0 3.996 0.268 52 N 48 49 58 0 MEAN 0.108 1.126 3.847 S.D. 0.012 0.084 0.332 36 0 3.841 0.222 56 N 48 48 48 0 0 0 MEAN 0.099 1.066 3.786 S.D. 0.025 0.173 0.474 60 N 45 48 47 0 0 0 MEAN 0.104 1.071 3.629 S.D. 0.025 0.164 0.481 64 N 43 45 44 0 0 0 MEAN 0.101 1.008 3.477 S.D. 0.022 0.153 0.492 215 Table 10 Covance 6329-212 3M T-6316.1 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------------MALE--------------------------------------- GROUP: 2 3 4 5 6 7 DOSE: 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 68 N 42 45 43 0 0 0 MEAN 0.100 0.997 3.343 S.D. 0.017 0.225 0.831 72 N 41 40 39 0 0 0 MEAN 0.107 1.035 3.705 S.D. 0.034 0.181 0.430 76 N 39 38 37 0 0 0 MEAN 0.095 1.004 3.529 S.D. 0.020 0.226 0.548 80 N 33 33 32 0 0 0 MEAN 0.095 1.010 3.626 S.D. 0.027 0.239 0.466 84 N 29 32 33 0 0 0 MEAN 0.103 1.057 3.310 S.D. 0.025 0.143 0.778 88 N 28 32 31 0 0 0 MEAN 0.098 0.957 3.285 S.D. 0.021 0.118 0.544 92 N 27 29 29 0 0 0 MEAN 0.092 0.952 3.204 S.D. 0.013 0.122 0.709 216 Table 10 Covance 6329-212 3M T-6316.1 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------------MALE--------------------------------------- GROUP: 2 3 4 5 6 7 DOSE: 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 96 N 25 25 24 0 0 0 MEAN 0.091 0.860 3.065 S.D. 0.013 0.210 0.600 100 N 22 24 21 0 0 0 MEAN 0.085 0.901 3.159 S.D. 0.020 0.134 0.542 104 N 19 22 14 0 0 0 MEAN 0.081 0.874 3.360 S.D. 0.019 0.142 0.664 217 Table 10 Covance 6329-212 3M T-6316.1 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------------FEMALE-------------------------------------- GROUP: 2 3 4 5 6 7 DOSE: 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 1 N 56 54 59 57 36 33 MEAN 0.307 3.070 10.166 28.712 10.135 29.270 S.D. 0.037 0.386 1.080 3.562 1.157 3.365 2 N 55 50 60 63 39 35 MEAN 0.286 2.791 9.372 25.653 9.093 26.381 S.D. 0.033 0.319 1.179 4.087 1.058 4.036 3 N 53 49 57 62 40 36 MEAN 0.271 2.595 8.509 22.650 8.376 22.505 S.D. 0.051 0.317 0.873 3.249 0.800 2.640 4 N 42 46 57 55 35 36 MEAN 0.256 2.540 8.314 21.385 7.946 21.593 S.D. 0.038 0.238 0.912 2.132 0.574 2.667 5 N 46 50 56 59 40 35 MEAN 0.239 2.442 7.581 20.872 7.644 20.702 S.D. 0.023 0.271 0.610 2.782 0.593 1.771 6 N 42 45 59 58 39 36 MEAN 0.234 2.312 7.316 20.229 7.115 20.300 S.D. 0.027 0.271 0.624 2.277 0.638 3.150 7 N 44 50 61 60 40 38 MEAN 0.219 2.183 6.942 19.568 6.839 20.004 S.D. 0.018 0.225 0.555 1.815 0.727 3.757 218 Table 10 Covance 6329-212 3M T-6316.1 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------------FEMALE-------------------------------------- GROUP: 2 3 4 5 6 7 DOSE: 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 8 N 45 44 57 0 MEAN 0.225 2.237 6.907 S.D. 0.040 0.390 0.675 33 0 7.017 0.722 9 N 47 48 60 0 MEAN 0.201 2.007 6.656 S.D. 0.029 0.203 1.398 39 0 6.458 0.446 10 N 44 47 60 0 MEAN 0.199 1.993 6.413 S.D. 0.027 0.228 0.760 37 0 6.488 0.543 11 N 44 49 62 0 MEAN 0.193 2.016 6.456 S.D. 0.035 0.342 1.352 37 0 6.508 0.898 12 N 43 51 62 0 MEAN 0.181 1.952 6.213 S.D. 0.023 0.246 0.720 38 0 6.312 0.861 13 N 47 54 64 0 MEAN 0.184 1.879 6.200 S.D. 0.031 0.275 1.400 39 0 6.045 0.705 14 N 45 45 57 0 MEAN 0.182 1.846 6.174 S.D. 0.031 0.155 1.263 38 0 6.058 0.429 219 Table 10 Covance 6329-212 3M T-6316.1 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------------FEMALE-------------------------------------- GROUP: 2 3 4 5 6 7 DOSE: 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 15 N 38 42 55 0 MEAN 0.179 1.873 6.130 S.D. 0.028 0.178 0.515 39 0 6.128 0.383 16 N 42 44 56 0 MEAN 0.177 1.841 6.010 S.D. 0.018 0.217 0.423 38 0 6.018 0.475 20 N 41 47 53 0 MEAN 0.178 1.844 6.163 S.D. 0.027 0.184 0.484 38 0 6.206 0.436 24 N 47 47 54 0 MEAN 0.183 1.782 6.214 S.D. 0.036 0.192 1.082 38 0 6.030 0.348 28 N 46 45 55 0 MEAN 0.170 1.696 5.916 S.D. 0.025 0.120 0.575 39 0 5.977 0.413 32 N 48 48 55 0 MEAN 0.172 1.739 6.162 S.D. 0.028 0.207 0.808 36 0 6.080 0.475 36 N 47 49 54 0 MEAN 0.175 1.732 6.349 S.D. 0.031 0.205 1.458 37 0 6.125 0.448 220 Table 10 Covance 6329-212 3M T-6316.1 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------------FEMALE-------------------------------------- GROUP: 2 3 4 5 6 7 DOSE: 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 40 N 48 49 56 0 MEAN 0.184 1.853 6.788 S.D. 0.027 0.258 1.158 38 0 6.631 0.448 44 N 48 50 56 0 MEAN 0.163 1.677 5.946 S.D. 0.020 0.195 0.888 40 0 5.912 0.783 48 N 49 50 56 0 MEAN 0.153 1.550 5.976 S.D. 0.018 0.215 1.169 39 0 5.715 0.425 52 N 46 48 55 0 MEAN 0.147 1.454 5.686 S.D. 0.018 0.250 0.899 39 0 5.355 0.552 56 N 47 47 47 0 0 0 MEAN 0.138 1.390 5.395 S.D. 0.020 0.215 0.844 60 N 46 45 44 0 0 0 MEAN 0.139 1.394 5.267 S.D. 0.018 0.203 0.531 64 N 46 45 43 0 0 0 MEAN 0.129 1.371 4.995 S.D. 0.034 0.209 0.636 221 Table 10 Covance 6329-212 3M T-6316.1 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------------FEMALE-------------------------------------- GROUP: 2 3 4 5 6 7 DOSE: 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 68 N 40 44 45 0 0 0 MEAN 0.137 1.409 5.151 S.D. 0.019 0.226 1.316 72 N 40 38 39 0 0 0 MEAN 0.136 1.402 5.016 S.D. 0.026 0.199 0.468 76 N 33 33 37 0 0 0 MEAN 0.137 1.353 5.178 S.D. 0.023 0.217 1.054 80 N 28 32 32 0 0 0 MEAN 0.131 1.350 5.107 S.D. 0.034 0.238 0.672 84 N 28 29 31 0 0 0 MEAN 0.127 1.276 4.949 S.D. 0.026 0.288 0.868 88 N 25 26 27 0 0 0 MEAN 0.119 1.179 4.659 S.D. 0.020 0.386 0.788 92 N 24 23 29 0 0 0 MEAN 0.109 1.205 4.674 S.D. 0.037 0.224 1.483 222 Table 10 Covance 6329-212 3M T-6316.1 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------------FEMALE-------------------------------------- GROUP: 2 3 4 5 6 7 DOSE: 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 96 N 20 22 24 0 0 0 MEAN 0.109 1.125 4.213 S.D. 0.031 0.198 1.173 100 N 14 17 23 0 0 0 MEAN 0.109 1.038 4.860 S.D. 0.027 0.212 1.582 104 N 13 15 18 0 0 0 MEAN 0.114 1.029 4.440 S.D. 0.022 0.212 1.039 223 Table 11 Summary of Clinical Hematology Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/.L G/DL % FL PG % X103/.L ------ -------- -------- -------- -------- -------- -------- -------- 0 MEAN 8.27 15.3 44.7 54.1 18.5 34.1 1269 S.D. .267 .44 1.43 1.10 .40 .61 111.0 N 5 5 5 5 5 5 5 300 MEAN S.D. N 6.61 * .609 5 12.7 * .68 5 37.1 * 2.14 5 56.3 2.65 5 19.2 .82 5 34.1 .72 5 1092 * 112.7 5 Covance 6329-212 3M T-6316.1 224 Table 11 Summary of Clinical Hematology Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 DOSE ppm WBC X103/.L N-SEG X103/.L LYMPH X103/.L MONO X103/.L EOSIN X103/.L BASO X103/.L N-SEG% LYMPH% MONO% EOSIN% BASO% ------ -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 8.5 1.2 6.9 .4 .1 .0 15 78 6 1 0 S.D. 3.27 .41 3.52 .29 .04 .00 6.1 8.8 4.0 .5 .0 N 5 5 5 5 5 5 5 5 5 5 5 300 MEAN 6.5 1.0 5.2 .3 .0 * .0 17 78 4 1 0 S.D. 1.98 .29 2.10 .19 .05 .00 11.7 12.1 2.9 .4 .0 N 5 5 5 5 5 5 5 5 5 5 5 225 Table 11 Summary of Clinical Hematology Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/.L G/DL % FL PG % X103/.L ------ -------- -------- -------- -------- -------- -------- -------- 0 MEAN 7.83 14.7 43.6 55.6 18.8 33.8 1114 S.D. .321 .40 1.62 1.13 .56 .48 88.8 N 5 5 5 5 5 5 5 300 MEAN S.D. N 6.83 * .388 5 13.0 * .81 5 37.9 * 2.43 5 55.5 1.31 5 19.0 .46 5 34.2 .29 5 1110 113.7 5 Covance 6329-212 3M T-6316.1 226 Table 11 Summary of Clinical Hematology Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 DOSE ppm WBC X103/.L N-SEG X103/.L LYMPH X103/.L MONO X103/.L EOSIN X103/.L BASO X103/.L N-SEG% LYMPH% MONO% EOSIN% BASO% ------ -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 7.7 .7 6.6 .3 .1 .0 10 84 4 2 0 S.D. 2.85 .31 2.95 .11 .09 .00 6.5 9.1 2.2 1.7 .0 N 5 5 5 5 5 5 5 5 5 5 5 300 MEAN 5.8 .6 5.0 .2 .0 * .0 10 86 3 1 0 S.D. .78 .30 .93 .10 .00 .00 6.4 5.7 1.1 .4 .0 N 5 5 5 5 5 5 5 5 5 5 5 227 Table 12 Summary of Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/.L G/DL % FL PG % X103/.L ------ -------- -------- -------- -------- -------- -------- -------- 0 MEAN 8.54 15.3 45.7 53.6 17.9 33.5 1240 S.D. .403 .48 1.34 1.46 .55 .53 156.0 N 10 10 10 10 10 10 10 3 MEAN 8.72 15.6 46.6 53.5 17.9 33.5 1140 S.D. .268 .45 1.17 1.21 .54 .47 67.8 N 10 10 10 10 10 10 10 30 MEAN S.D. N 8.33 .455 10 15.0 .71 10 44.6 1.87 10 53.6 1.45 10 18.0 .38 10 33.7 .37 10 1143 158.3 10 100 MEAN S.D. N 8.18 .347 10 15.0 .43 10 44.4 1.57 10 54.4 1.23 10 18.4 .51 10 33.8 .42 10 1141 80.6 10 Covance 6329-212 3M T-6316.1 228 Table 12 Summary of Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 DOSE ppm WBC X103/.L N-SEG X103/.L LYMPH X103/.L MONO X103/.L EOSIN X103/.L BASO X103/.L N-SEG% LYMPH% MONO% EOSIN% BASO% ------ -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 7.4 1.2 5.3 .7 .1 .0 17 71 10 2 0 S.D. 1.31 .38 1.27 .19 .04 .00 5.2 7.1 2.8 .6 .0 N 10 10 10 10 10 10 10 10 10 10 10 3 MEAN 10.4 1.5 7.9 .8 .2 .0 15 75 7 2 0 S.D. 3.31 .54 2.79 .29 .11 .00 4.6 6.6 2.3 .7 .0 N 10 10 10 10 10 10 10 10 10 10 10 30 MEAN 9.2 1.3 7.0 .7 .2 .0 15 75 8 2 0 S.D. 1.85 .41 1.59 .28 .07 .00 4.6 4.7 2.4 .6 .0 N 10 10 10 10 10 10 10 10 10 10 10 100 MEAN 8.8 1.3 6.7 .7 .1 .0 15 76 8 1 0 S.D. 2.64 .39 2.23 .24 .05 .00 2.6 3.3 2.5 .5 .0 N 10 10 10 10 10 10 10 10 10 10 10 229 Table 12 Summary of Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/.L G/DL % FL PG % X103/.L ------ -------- -------- -------- -------- -------- -------- -------- 0 MEAN 8.15 15.5 45.9 56.4 19.0 33.7 1040 S.D. .469 .65 2.09 1.17 .61 .46 113.7 N 10 10 10 10 10 10 10 3 MEAN 8.12 15.3 45.3 55.8 18.8 33.7 1000 S.D. .411 .64 2.16 1.97 .67 .38 222.8 N 10 10 10 10 10 10 10 30 MEAN S.D. N 8.01 .419 10 15.2 .55 10 45.5 1.63 10 56.8 1.72 10 19.1 .80 10 33.6 .45 10 1054 124.0 10 100 MEAN S.D. N 7.79 .258 9 14.9 .23 9 43.7 .82 9 56.2 1.46 9 19.2 .61 9 34.1 .54 9 1023 56.2 9 Covance 6329-212 3M T-6316.1 230 Table 12 Summary of Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 DOSE ppm WBC X103/.L N-SEG X103/.L LYMPH X103/.L MONO X103/.L EOSIN X103/.L BASO X103/.L N-SEG% LYMPH% MONO% EOSIN% BASO% ------ -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 6.4 .8 5.1 .4 .1 .0 13 79 6 2 0 S.D. 1.63 .22 1.44 .13 .04 .00 3.6 4.2 1.4 .6 .0 N 10 10 10 10 10 10 10 10 10 10 10 3 MEAN 6.9 .8 5.5 .4 .1 .0 11 81 6 2 0 S.D. 1.77 .43 1.48 .12 .03 .00 4.5 4.8 2.0 .8 .0 N 10 10 10 10 10 10 10 10 10 10 10 30 MEAN 7.5 .8 6.3 .4 .1 .0 12 80 5 2 0 S.D. 5.43 .22 5.06 .19 .09 .00 5.5 5.9 1.8 .5 .0 N 10 10 10 10 10 10 10 10 10 10 10 100 MEAN 6.2 .6 5.1 .4 .1 .0 11 80 6 2 0 S.D. 1.80 .26 1.68 .12 .06 .00 4.8 6.6 3.0 .7 .0 N 9 9 9 9 9 9 9 9 9 9 9 231 Table 13 Summary of Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/.L G/DL % FL PG % X103/.L ------ -------- -------- -------- -------- -------- -------- -------- 0 MEAN 8.33 14.8 44.2 53.1 17.8 33.4 1270 S.D. .445 .47 1.41 1.63 .66 .40 107.3 N 10 10 10 10 10 10 10 3 MEAN 8.19 14.6 44.0 53.8 17.9 33.3 1096 * S.D. .879 1.46 4.15 1.83 .66 .45 100.5 N 10 10 10 10 10 10 10 30 MEAN S.D. N 8.27 .374 10 14.9 .51 10 44.0 1.12 10 53.2 1.98 10 18.0 .78 10 33.8 .44 10 1190 131.4 10 100 MEAN S.D. N 8.22 .398 10 14.5 .59 10 42.9 2.12 10 52.2 2.24 10 17.6 .77 10 33.7 .44 10 1206 55.6 10 Covance 6329-212 3M T-6316.1 232 Table 13 Summary of Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 DOSE ppm WBC X103/.L N-SEG X103/.L LYMPH X103/.L MONO X103/.L EOSIN BASO X103/.L X103/.L N-SEG% LYMPH% MONO% EOSIN% BASO% ------ -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 7.9 1.4 5.5 .8 .2 .0 19 69 10 2 0 S.D. 1.77 .34 1.52 .19 .06 .00 4.2 4.7 1.8 .4 .0 N 10 10 10 10 10 10 10 10 10 10 10 3 MEAN 7.9 1.4 5.6 .7 .2 .0 17 71 9 2 0 S.D. 1.62 .65 1.14 .23 .08 .00 4.5 5.3 4.1 .7 .3 N 10 10 10 10 10 10 10 10 10 10 10 30 MEAN 8.8 2.0 5.8 .8 .2 .0 22 68 9 2 0 S.D. 1.91 .98 1.22 .27 .07 .00 8.3 8.7 2.2 .8 .0 N 10 10 10 10 10 10 10 10 10 10 10 100 MEAN 8.9 2.4 5.6 .8 .1 .0 24 65 9 2 0 S.D. 3.06 2.34 1.27 .28 .04 .00 11.6 11.7 2.4 .7 .3 N 10 10 10 10 10 10 10 10 10 10 10 233 Table 13 Summary of Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/.L G/DL % FL PG % X103/.L ------ -------- -------- -------- -------- -------- -------- -------- 0 MEAN 7.93 15.3 45.1 56.9 19.3 33.9 1076 S.D. .316 .29 1.04 1.76 .76 .38 96.6 N 10 10 10 10 10 10 10 3 MEAN 7.54 * 14.7 * 43.2 * 57.4 19.5 34.0 S.D. .243 .50 1.23 1.88 .82 .49 N 10 10 10 10 10 10 987 176.1 10 30 MEAN S.D. N 7.78 .396 10 14.9 .46 10 43.9 1.38 10 56.4 1.99 10 19.1 .80 10 33.9 .49 10 968 94.2 10 100 MEAN S.D. N 7.25 * .268 10 14.1 * .61 10 41.0 * 1.82 10 56.5 1.74 10 19.4 .51 10 34.4 .56 10 996 103.9 10 Covance 6329-212 3M T-6316.1 234 Table 13 Summary of Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T6316.1 DOSE ppm WBC X103/.L N-SEG X103/.L LYMPH X103/.L MONO X103/.L EOSIN BASO X103/.L X103/.L N-SEG% LYMPH% MONO% EOSIN% BASO% ------ -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 5.1 .7 3.7 .6 .1 .0 14 71 12 2 0 S.D. 1.29 .17 1.39 .21 .00 .03 5.2 10.3 5.5 .6 .7 N 10 10 10 10 10 10 10 10 10 10 10 3 MEAN 5.2 .7 3.9 .5 .1 .0 14 74 10 2 0 S.D. 1.85 .24 1.55 .17 .03 .00 2.8 4.0 3.2 .9 .3 N 10 10 10 10 10 10 10 10 10 10 10 30 MEAN 4.8 .8 3.3 .5 .1 .0 18 69 11 2 0 S.D. 1.20 .22 .98 .21 .03 .00 4.6 7.5 4.1 .7 .4 N 10 10 10 10 10 10 10 10 10 10 10 100 MEAN 4.9 .8 3.5 .4 .1 .0 17 72 8 2 0 S.D. .69 .28 .73 .07 .03 .00 5.7 6.7 2.2 .5 .4 N 10 10 10 10 10 10 10 10 10 10 10 235 Table 14 Summary of Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/.L G/DL % FL PG % X103/.L ------ -------- -------- -------- -------- -------- -------- -------- 0 MEAN 8.01 14.5 43.3 54.2 18.2 33.4 1238 S.D. 1.004 1.52 4.50 2.32 .78 .30 229.3 N 10 10 10 10 10 10 10 3 MEAN 8.42 15.2 45.3 53.8 18.1 33.6 1210 S.D. .498 .76 2.79 1.88 .52 .61 176.3 N 10 10 10 10 10 10 10 30 MEAN S.D. N 8.39 .585 10 14.8 1.12 10 43.8 3.17 10 52.2 1.82 10 17.6 .80 10 33.8 .56 10 1199 97.5 10 100 MEAN S.D. N 8.45 .454 10 14.7 .68 10 43.3 1.73 10 51.3 * 1.06 10 17.4 * .45 10 33.9 .55 10 1129 184.1 10 Covance 6329-212 3M T6316.1 236 Table 14 Summary of Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T6316.1 DOSE ppm WBC X103/.L N-SEG X103/.L LYMPH X103/.L MONO X103/.L EOSIN BASO X103/.L X103/.L N-SEG% LYMPH% MONO% EOSIN% BASO% ------ -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 8.5 2.4 4.9 1.0 .2 .0 29 58 12 2 0 S.D. 2.14 .88 1.26 .35 .08 .00 5.1 5.4 3.4 .7 .3 N 10 10 10 10 10 10 10 10 10 10 10 3 MEAN 7.9 1.9 4.9 .9 .1 .0 24 62 11 2 0 S.D. 1.11 .73 .70 .28 .05 .03 6.9 6.6 3.2 .7 .3 N 10 10 10 10 10 10 10 10 10 10 10 30 MEAN 8.8 2.5 5.5 .7 .2 .0 28 62 8 2 0 S.D. .90 .79 1.01 .43 .07 .00 8.3 8.8 4.6 .7 .0 N 10 10 10 10 10 10 10 10 10 10 10 100 MEAN 7.5 1.8 4.7 .8 .2 .0 25 62 11 2 0 S.D. 2.02 .53 1.74 .19 .05 .00 7.1 9.5 3.6 .6 .3 N 10 10 10 10 10 10 10 10 10 10 10 237 Table 14 Summary of Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/.L G/DL % FL PG % X103/.L ------ -------- -------- -------- -------- -------- -------- -------- 0 MEAN 7.23 14.2 41.5 58.2 20.0 34.4 S.D. 1.388 1.95 6.15 4.19 1.81 .64 N 10 10 10 10 10 10 998 125.1 10 3 MEAN 7.42 14.3 42.4 57.2 19.3 33.7 * 935 S.D. .589 1.03 2.99 1.78 .85 .56 125.9 N 10 10 10 10 10 10 10 30 MEAN S.D. N 7.44 .979 10 14.6 1.86 10 42.8 5.57 10 57.6 2.13 10 19.6 .83 10 34.0 .51 10 918 133.9 10 100 MEAN S.D. N 6.92 1.008 10 13.6 1.62 10 39.4 4.74 10 57.1 2.24 10 19.7 .79 10 34.6 .43 10 907 80.9 10 Covance 6329-212 3M T6316.1 238 Table 14 Summary of Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T6316.1 DOSE ppm WBC X103/.L N-SEG X103/.L LYMPH X103/.L MONO X103/.L EOSIN BASO X103/.L X103/.L N-SEG% LYMPH% MONO% EOSIN% BASO% ------ -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 4.5 1.1 2.7 .6 .1 .0 25 60 13 2 0 S.D. .59 .39 .40 .08 .03 .00 6.8 6.8 1.2 .5 .4 N 10 10 10 10 10 10 10 10 10 10 10 3 MEAN 4.8 1.5 2.6 .6 .1 .0 26 59 12 2 0 S.D. 1.95 1.60 .74 .34 .03 .03 13.8 14.8 1.9 .9 .3 N 10 10 10 10 10 10 10 10 10 10 10 30 MEAN 5.7 1.2 3.8 .5 .1 .0 22 66 9 3 0 S.D. 1.60 .49 1.43 .20 .07 .00 7.4 8.4 3.8 1.4 .0 N 10 10 10 10 10 10 10 10 10 10 10 100 MEAN 5.8 * 1.6 3.4 .7 .2 .0 28 58 11 3 0 S.D. .74 .33 .63 .19 .08 .00 4.6 5.7 4.0 1.2 .3 N 10 10 10 10 10 10 10 10 10 10 10 239 Table 15 Summary of Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G ------ -------- 0 MEAN 7 S.D. 3.8 N 5 3 MEAN 8 S.D. 2.5 N 5 30 MEAN 9 S.D. 1.7 N 5 100 MEAN 6 S.D. 1.9 N 5 300 MEAN S.D. N 14 * 3.6 5 Covance 6329-212 3M T-6316.1 240 Table 15 Summary of Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G ------ -------- 0 MEAN 3 S.D. 2.7 N 5 3 MEAN 5 S.D. 1.6 N 5 30 MEAN 4 S.D. 2.7 N 5 100 MEAN 5 S.D. 1.6 N 5 300 MEAN S.D. N 15 * 7.9 5 Covance 6329-212 3M T-6316.1 241 Table 16 Summary of Clinical Chemistry Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 106 13 .8 6.7 4.6 2.2 .1 53 S.D. 6.3 1.9 .13 .48 .19 .31 .08 13.3 N 5 5 5 5 5 5 5 5 300 MEAN 94 * 18 * .7 7.1 5.5 * 1.6 * .5 * 7* S.D. 6.2 2.2 .08 .61 .38 .30 .09 5.2 N 5 5 5 5 5 5 5 5 Covance 6329-212 3M T-6316.1 242 Table 16 Summary of Clinical Chemistry Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 113 24.8 5 42 7.0 5 2 10.5 1.6 .21 5 5 7.8 .79 5 148 1.5 5 5.7 .26 5 103 1.9 5 300 MEAN S.D. N 179 * 55.6 5 271 * 213.8 5 1 10.4 1.2 .26 5 5 7.7 .34 5 147 1.6 5 5.6 .32 5 103 2.2 5 Covance 6329-212 3M T-6316.1 243 Table 16 Summary of Clinical Chemistry Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G ------ -------- 0 MEAN 9 S.D. 3.5 N 5 300 MEAN S.D. N 26 * 5.3 5 Covance 6329-212 3M T-6316.1 244 Table 16 Summary of Clinical Chemistry Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 109 13 .8 7.3 5.4 1.9 .3 69 S.D. 8.8 2.3 .11 .31 .29 .19 .09 16.2 N 5 5 5 5 5 5 5 5 300 MEAN 86 * 22 * .8 7.7 6.2 * 1.5 .4 11 * S.D. 20.0 3.7 .10 1.00 .63 .40 .08 4.0 N 5 5 5 5 5 5 5 5 Covance 6329-212 3M T-6316.1 245 Table 16 Summary of Clinical Chemistry Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 129 21.6 5 42 19.8 5 1 11.1 1.1 .38 5 5 8.4 1.99 5 148 2.3 5 5.9 .61 5 103 2.6 5 300 MEAN S.D. N 145 78.4 5 94 67.7 5 1 11.2 1.7 .40 5 5 8.6 1.22 5 149 2.8 5 5.6 .34 5 105 1.5 5 Covance 6329-212 3M T-6316.1 246 Table 16 Summary of Clinical Chemistry Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G ------ -------- 0 MEAN 3 S.D. 2.7 N 5 300 MEAN S.D. N 26 * 6.6 5 Covance 6329-212 3M T-6316.1 247 Table 17 Summary of Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 111 13 .7 7.4 5.0 2.4 .2 82 S.D. 18.0 1.4 .13 .32 .16 .23 .12 14.4 N 10 10 10 10 10 10 10 10 3 MEAN 101 14 .7 7.1 4.7 * 2.4 .2 61 * S.D. 5.7 2.2 .06 .28 .14 .21 .08 6.8 N 10 10 10 10 10 10 10 10 30 MEAN 102 14 .7 7.1 4.8 2.4 .1 53 * S.D. 8.6 2.0 .14 .36 .18 .31 .10 7.9 N 10 10 10 10 10 10 10 10 100 MEAN 94 * 15 .6 7.2 5.1 2.2 .2 29 * S.D. 7.0 1.6 .05 .31 .25 .25 .08 9.8 N 10 10 10 10 10 10 10 10 Covance 6329-212 3M T-6316.1 248 Table 17 Summary of Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 128 32.7 10 41 6.9 10 0 .4 10 10.8 .37 10 7.7 .62 10 149 1.7 10 6.5 .41 10 105 1.3 10 3 MEAN S.D. N 130 27.0 10 42 6.1 10 0 .7 10 10.6 .28 10 7.8 .69 10 148 1.7 10 6.2 .28 10 104 2.4 10 30 MEAN S.D. N 116 31.0 10 46 14.2 10 0 .3 10 10.8 .28 10 7.8 .55 10 148 1.5 10 6.1 * .34 10 106 2.1 10 100 MEAN S.D. N 111 32.3 10 47 11.1 10 0 1.3 10 10.6 .35 10 7.7 .42 10 148 1.7 10 6.1 * .35 10 106 2.4 10 Covance 6329-212 3M T-6316.1 249 Table 17 Summary of Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 106 13 .8 8.3 6.1 2.2 .2 95 S.D. 10.4 1.5 .12 .47 .43 .21 .10 29.6 N 10 10 10 10 10 10 10 10 3 MEAN 108 14 .7 8.0 6.0 2.0 .2 80 S.D. 7.9 2.3 .10 .39 .43 .12 .18 12.9 N 10 10 10 10 10 10 10 10 30 MEAN 97 16 .8 7.8 5.8 2.0 .1 69 * S.D. 11.9 3.8 .16 .53 .37 .24 .13 18.3 N 10 10 10 10 10 10 10 10 100 MEAN 94 * 19 * .7 8.1 6.1 2.0 .2 58 * S.D. 9.9 4.3 .12 .31 .19 .32 .12 22.9 N 10 10 10 10 10 10 10 10 Covance 6329-212 3M T-6316.1 250 Table 17 Summary of Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 110 30.2 10 39 18.5 10 0 1.0 10 11.3 .37 10 7.0 1.22 10 149 2.4 10 6.0 .80 10 106 2.0 10 3 MEAN S.D. N 96 14.8 10 32 6.5 10 0 .5 10 11.4 .38 10 7.3 .95 10 148 1.8 10 6.1 .59 10 105 1.2 10 30 MEAN S.D. N 118 16.0 10 34 6.0 10 0 .5 10 10.8 * .46 10 6.8 1.00 10 149 2.2 10 5.8 .35 10 106 1.4 10 100 MEAN S.D. N 87 17.1 10 33 4.7 10 1 1.5 10 11.1 .27 10 6.8 .74 10 149 2.0 10 5.9 .37 10 106 2.2 10 Covance 6329-212 3M T-6316.1 251 Table 18 Summary of Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 106 11 .6 7.4 4.6 2.7 .1 96 S.D. 11.8 1.2 .13 .31 .25 .13 .13 30.3 N 10 10 10 10 10 10 10 10 3 MEAN 108 13 .7 7.4 4.8 2.6 .2 86 S.D. 12.1 1.8 .12 .43 .39 .19 .11 19.8 N 10 10 10 10 10 10 10 10 30 MEAN S.D. N 98 13 .7 7.2 4.7 2.5 * .1 6.2 1.7 .10 .51 .39 .20 .10 10 10 10 10 10 10 10 68 * 18.8 10 100 MEAN S.D. N 95 * 15 * .6 7.7 5.3 * 2.4 * .1 7.3 1.6 .11 .32 .25 .28 .11 10 10 10 10 10 10 10 42 * 16.2 10 Covance 6329-212 3M T-6316.1 252 Table 18 Summary of Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 114 28.0 10 40 13.8 10 1 1.3 10 10.8 .38 10 7.4 .82 10 149 1.9 10 6.7 .48 10 106 1.1 10 3 MEAN S.D. N 119 38.3 10 42 21.2 10 0 .7 10 10.6 .39 10 6.9 .66 10 147 2.3 10 6.2 .48 10 105 2.4 10 30 MEAN S.D. N 114 38.7 10 57 31.0 10 1 1.0 10 10.6 .34 10 7.2 .40 10 147 1.4 10 6.3 .37 10 106 1.2 10 100 MEAN S.D. N 165 192.1 10 94 113.7 10 1 .8 10 10.7 .32 10 7.2 .40 10 147 1.4 10 6.3 .35 10 106 1.1 10 Covance 6329-212 3M T-6316.1 253 Table 18 Summary of Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 101 15 .8 8.0 5.8 2.2 .2 87 S.D. 13.4 3.7 .24 .65 .53 .23 .10 14.3 N 10 10 10 10 10 10 10 10 3 MEAN 103 14 .7 8.2 5.8 2.4 S.D. 7.3 2.2 .11 .54 .57 .28 N 10 10 10 10 10 10 .2 104 .14 21.4 10 10 30 MEAN 107 16 .6 8.0 5.8 2.2 .2 86 S.D. 8.9 3.7 .08 .61 .41 .28 .12 19.6 N 10 10 10 10 10 10 10 10 100 MEAN 92 21 * .8 8.5 6.2 2.4 .2 71 S.D. 7.1 7.0 .21 .50 .30 .25 .14 20.6 N 10 10 10 10 10 10 10 10 Covance 6329-212 3M T-6316.1 254 Table 18 Summary of Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 122 30.0 10 44 19.3 10 1 1.1 10 11.0 .41 10 6.0 .44 10 147 2.9 10 6.1 .57 10 105 2.0 10 3 MEAN S.D. N 102 23.6 10 41 17.7 10 2 4.7 10 11.0 .41 10 5.8 .76 10 147 .9 10 5.7 .54 10 105 1.4 10 30 MEAN S.D. N 104 22.1 10 37 10.8 10 2 1.4 10 11.0 .53 10 6.1 .92 10 147 1.7 10 6.1 .69 10 107 1.3 10 100 MEAN S.D. N 105 42.4 10 47 17.3 10 1 1.3 10 11.2 .24 10 6.2 .63 10 146 1.6 10 5.8 .71 10 104 2.1 10 Covance 6329-212 3M T-6316.1 255 Table 19 Summary of Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 115 13 .6 7.3 4.4 2.9 S.D. 11.7 1.5 .10 .38 .25 .45 N 10 10 10 10 10 10 .1 146 .10 70.8 10 10 3 MEAN 111 12 .6 7.6 4.7 2.9 S.D. 10.8 1.6 .11 .67 .49 .48 N 10 10 10 10 10 10 .2 119 .13 36.1 10 10 30 MEAN 116 14 .7 7.3 4.7 2.6 .2 92 * S.D. 9.4 2.2 .18 .32 .18 .35 .13 38.3 N 10 10 10 10 10 10 10 10 100 MEAN 113 14 .6 7.7 5.3 * 2.4 * .1 S.D. 13.7 1.0 .11 .35 .30 .27 .09 N 10 10 10 10 10 10 10 49 * 21.4 10 Covance 6329-212 3M T-6316.1 256 Table 19 Summary of Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 117 23.7 10 40 9.4 10 4 3.5 10 11.1 .41 10 6.5 .87 10 150 1.7 10 6.4 .36 10 109 2.3 10 3 MEAN S.D. N 108 22.9 10 48 14.4 10 3 2.7 10 11.0 .35 10 6.7 .53 10 150 1.6 10 6.5 .52 10 108 1.3 10 30 MEAN S.D. N 105 25.4 10 51 19.2 10 4 4.5 10 10.9 .30 10 6.8 .62 10 150 1.9 10 6.5 .42 10 109 1.6 10 100 MEAN S.D. N 83 * 16.2 10 68 * 26.5 10 6 4.2 10 10.9 .20 10 6.5 .37 10 148 * 1.6 10 6.3 .45 10 109 2.0 10 Covance 6329-212 3M T-6316.1 257 Table 19 Summary of Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 108 13 .7 8.0 5.9 2.1 S.D. 14.0 3.0 .12 .70 .58 .25 N 10 10 10 10 10 10 .3 116 .16 22.1 10 10 3 MEAN 105 13 .7 8.2 5.8 2.5 S.D. 13.0 1.6 .17 .55 .57 .40 N 10 10 10 10 10 10 .2 119 .19 39.1 10 10 30 MEAN 93 * 14 .8 8.4 5.9 2.4 .3 94 S.D. 7.8 2.1 .09 .37 .22 .33 .25 23.9 N 10 10 10 10 10 10 10 10 100 MEAN 103 16 .6 8.5 5.9 2.6 * .1 S.D. 11.3 4.2 .08 .42 .22 .37 .12 N 10 10 10 10 10 10 10 88 23.2 10 Covance 6329-212 3M T-6316.1 258 Table 19 Summary of Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 107 33.7 10 43 16.5 10 2 1.8 10 11.1 .44 10 5.8 .73 10 148 2.2 10 5.6 .51 10 106 3.5 10 3 MEAN S.D. N 104 27.4 10 48 22.0 10 2 2.8 10 11.2 .43 10 5.8 .44 10 149 1.7 10 6.1 .45 10 108 2.8 10 30 MEAN S.D. N 125 56.9 10 66 72.0 10 5 6.8 10 11.1 .42 10 6.2 .85 10 150 3.9 10 5.9 .56 10 107 2.4 10 100 MEAN S.D. N 89 19.9 10 63 26.1 10 4 4.0 10 11.3 .29 10 5.8 .48 10 147 1.9 10 5.7 .40 10 107 2.9 10 Covance 6329-212 3M T-6316.1 259 Table 20 Summary of Clinical Chemistry Data Males Week 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm CHOL MG/DL TRIG MG/DL ------ -------- -------- 0 MEAN S.D. N 152 65.0 14 189 119.3 14 3 MEAN S.D. N 144 83.4 22 199 162.2 22 30 MEAN S.D. N 109 33.4 22 127 73.2 22 100 MEAN S.D. N 81 * 48.7 17 110 111.2 17 100 MEAN S.D. N 120 68.7 10 173 120.7 10 Covance 6329-212 3M T-6316.1 260 Table 20 Summary of Clinical Chemistry Data Females Week 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm CHOL MG/DL TRIG MG/DL ------ -------- -------- 0 MEAN S.D. N 116 32.6 13 166 97.3 13 3 MEAN S.D. N 109 24.9 13 175 128.4 13 30 MEAN S.D. N 100 22.8 15 116 97.6 15 100 MEAN S.D. N 108 44.8 19 90 * 88.3 19 100 MEAN S.D. N 154 * 53.4 12 482 469.7 12 Covance 6329-212 3M T-6316.1 261 Table 21 Summary of Clinical Urinalysis Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH ------ -------- -------- -------- 0 MEAN 17.5 1.020 S.D. 10.64 .0086 N 5 5 7.0 .35 5 300 MEAN S.D. N 28.5 10.88 5 1.010 .0047 5 6.9 .22 5 Covance 6329-212 3M T-6316.1 262 Table 21 Summary of Clinical Urinalysis Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH ------ -------- -------- -------- 0 MEAN 19.9 1.012 S.D. 17.75 .0102 N 5 5 6.8 .27 5 300 MEAN S.D. N 21.6 12.63 5 1.010 .0051 5 6.7 .45 5 Covance 6329-212 3M T-6316.1 263 Table 22 Summary of Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH ------ -------- -------- -------- 0 MEAN 18.7 1.024 S.D. 14.79 .0151 N 10 10 6.9 .39 10 3 MEAN 25.1 1.015 S.D. 15.47 .0079 N 10 10 6.9 .21 10 30 MEAN S.D. N 15.8 9.53 10 1.022 .0109 10 6.8 .26 10 100 MEAN S.D. N 19.9 15.56 10 1.022 .0160 10 6.8 .35 10 Covance 6329-212 3M T-6316.1 264 Table 22 Summary of Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH ------ -------- -------- -------- 0 MEAN 19.0 1.012 S.D. 13.21 .0082 N 10 10 6.7 .26 10 3 MEAN 22.2 1.012 S.D. 16.26 .0102 N 10 10 6.8 .26 10 30 MEAN S.D. N 19.2 16.50 10 1.014 .0109 10 6.6 .39 10 100 MEAN S.D. N 23.2 16.13 10 1.012 .0086 10 6.6 .16 10 Covance 6329-212 3M T-6316.1 265 Table 23 Summary of Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH ------ -------- -------- -------- 0 MEAN 15.5 1.024 S.D. 8.17 .0087 N 10 10 7.0 .33 10 3 MEAN 13.7 1.025 S.D. 5.65 .0090 N 10 10 7.2 .35 10 30 MEAN S.D. N 13.7 13.33 10 1.030 .0137 10 7.0 .33 10 100 MEAN S.D. N 15.4 13.36 10 1.025 .0100 10 7.0 .33 10 Covance 6329-212 3M T-6316.1 266 Table 23 Summary of Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH ------ -------- -------- -------- 0 MEAN 18.1 1.019 S.D. 16.96 .0140 N 10 10 6.9 .32 10 3 MEAN 15.9 1.016 S.D. 15.38 .0100 N 10 10 6.8 .41 10 30 MEAN S.D. N 13.0 13.35 10 1.020 .0190 10 6.7 .35 10 100 MEAN S.D. N 13.2 8.01 10 1.019 .0081 10 6.8 .49 10 Covance 6329-212 3M T-6316.1 267 Table 24 Summary of Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH ------ -------- -------- -------- 0 MEAN 19.3 1.024 S.D. 15.90 .0092 N 10 10 6.8 .26 10 3 MEAN 13.7 1.029 S.D. 6.72 .0106 N 10 10 7.0 .33 10 30 MEAN S.D. N 14.7 7.46 10 1.026 .0078 10 6.8 .35 10 100 MEAN S.D. N 17.3 12.78 10 1.023 .0110 10 6.8 .34 10 Covance 6329-212 3M T-6316.1 268 Table 24 Summary of Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH ------ -------- -------- -------- 0 MEAN 13.1 1.018 S.D. 7.81 .0074 N 10 10 6.4 .28 10 3 MEAN 19.3 1.018 S.D. 17.82 .0122 N 10 10 6.4 .16 10 30 MEAN S.D. N 13.7 12.84 10 1.021 .0101 10 6.4 .24 10 100 MEAN S.D. N 17.0 14.38 10 1.021 .0131 10 6.4 .16 10 Covance 6329-212 3M T-6316.1 269 Table 25 Summary of Urine Chemistry Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL ------ -------- -------- -------- -------- 0 MEAN S.D. N 27 13.5 5 120 36.4 5 .37 .135 5 1.99 1.394 5 300 MEAN S.D. N 20 14.2 5 41 * 18.8 5 .53 .213 4 1.10 .403 4 Covance 6329-212 3M T-6316.1 270 Table 25 Summary of Urine Chemistry Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL ------ -------- -------- -------- -------- 0 MEAN S.D. N 16 11.4 5 53 47.3 5 .15 .074 4 .46 .164 4 300 MEAN S.D. N 20 9.9 5 42 18.0 5 .38 .178 4 .76 * .113 4 Covance 6329-212 3M T-6316.1 271 Table 26 Summary of Urine Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL ------ -------- -------- -------- -------- 0 MEAN S.D. N 28 15.9 10 155 111.3 10 .35 .159 10 1.70 .469 10 3 MEAN S.D. N 22 14.5 10 82 51.3 10 .40 .197 10 1.40 .521 10 30 MEAN S.D. N 30 24.4 10 127 88.4 10 .32 .108 10 1.44 .405 10 100 MEAN S.D. N 29 28.2 10 128 115.5 10 .31 .071 10 1.35 .335 10 Covance 6329-212 3M T-6316.1 272 Table 26 Summary of Urine Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL ------ -------- -------- -------- -------- 0 MEAN S.D. N 19 12.0 10 61 47.7 10 .25 .062 10 .74 .256 10 3 MEAN S.D. N 21 16.5 10 70 73.0 10 .26 .139 10 .66 .214 10 30 MEAN S.D. N 18 11.0 10 73 76.5 10 .22 .132 10 .65 .244 10 100 MEAN S.D. N 20 15.4 10 57 51.3 10 .31 .098 10 .72 .271 10 Covance 6329-212 3M T-6316.1 273 Table 27 Summary of Urine Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL ------ -------- -------- -------- -------- 0 MEAN S.D. N 28 15.9 10 102 47.8 10 .37 .227 10 1.30 .281 10 3 MEAN S.D. N 34 11.2 10 102 34.0 10 .46 .227 10 1.26 .274 10 30 MEAN S.D. N 46 20.5 10 135 76.2 10 .43 .197 10 1.12 .266 10 100 MEAN S.D. N 43 25.3 10 114 56.2 10 .46 .186 9 1.26 .413 9 Covance 6329-212 3M T-6316.1 274 Table 27 Summary of Urine Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL ------ -------- -------- -------- -------- 0 MEAN S.D. N 23 16.1 10 85 58.0 10 .24 .164 8 .81 .318 8 3 MEAN S.D. N 29 22.9 10 66 43.7 10 .26 .148 9 .61 .173 9 30 MEAN S.D. N 21 10.7 9 52 20.2 9 .24 .133 8 .57 .229 8 100 MEAN S.D. N 27 20.8 10 78 38.4 10 .27 .098 10 .81 .191 10 Covance 6329-212 3M T-6316.1 275 Table 28 Summary of Urine Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL ------ -------- -------- -------- -------- 0 MEAN S.D. N 23 9.5 10 110 52.5 10 .28 .077 8 1.50 .296 8 3 MEAN S.D. N 27 7.2 10 141 60.8 10 .33 .102 10 1.62 .273 10 30 MEAN S.D. N 28 11.2 10 136 61.5 10 .39 .221 10 1.63 .462 10 100 MEAN S.D. N 28 12.5 10 116 58.9 10 .38 .142 9 1.45 .335 9 Covance 6329-212 3M T-6316.1 276 Table 28 Summary of Urine Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL ------ -------- -------- -------- -------- 0 MEAN S.D. N 29 16.8 10 88 40.9 10 .33 .206 10 .91 .240 10 3 MEAN S.D. N 29 23.4 10 92 68.9 10 .26 .086 8 .87 .283 8 30 MEAN S.D. N 24 12.6 10 101 60.4 10 .24 .107 9 .98 .395 9 100 MEAN S.D. N 31 27.4 10 113 82.0 10 .28 .107 10 1.01 .329 10 Covance 6329-212 3M T-6316.1 277 Table 29 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 4 Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=1;SUBSET=T LIVER -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 MEAN: 350.7 10.8223 3.0744 STANDARD DEV: 33.3 1.5753 0.1520 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 MEAN: 196.1 6.4594 3.3170 STANDARD DEV: 20.3 0.4155 0.3676 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 MEAN: 362.8 10.7856 2.9699 STANDARD DEV: 32.5 1.1739 0.1104 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 MEAN: 209.0 6.7264 3.2329 STANDARD DEV: 29.8 0.7320 0.1459 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 MEAN: 344.4 10.9453 3.1683 STANDARD DEV: 28.5 1.5396 0.2257 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 MEAN: 198.8 6.8324 3.4373 STANDARD DEV: 17.2 0.6354 0.1362 --------------------------------------------------------------- --------------------------------------------------------------- 278 Table 29 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 4 Sacrifice PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=1;SUBSET=T LIVER -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 MEAN: 343.8 12.2371 3.5708 STANDARD DEV: 36.4 0.9341 0.2090 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 MEAN: 310.0 15.7639 5.0860 STANDARD DEV: 28.3 1.4058 0.1240 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 MEAN: 191.3 7.1709 3.7577 STANDARD DEV: 13.1 0.5706 0.3444 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 MEAN: 181.6 9.2338 5.1048 STANDARD DEV: 15.1 1.4896 0.8198 --------------------------------------------------------------- 279 Table 30 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL LEFT ADRENAL -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 442.3 0.0318 0.0072 0.0146 STANDARD DEV: 31.8 0.0042 0.0013 0.0020 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 257.4 0.0400 0.0155 0.0198 STANDARD DEV: 31.4 0.0077 0.0018 0.0036 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 317.3 * 0.0278 0.0088 0.0136 STANDARD DEV: 50.6 0.0056 0.0018 0.0029 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 170.3 * 0.0304 * 0.0179 0.0167 STANDARD DEV: 14.1 0.0043 0.0021 0.0023 --------------------------------------------------------------- --------------------------------------------------------------- 280 Table 30 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL RIGHT ADRENAL -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 442.3 0.0295 0.0067 0.0136 STANDARD DEV: 31.8 0.0029 0.0011 0.0014 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 257.4 0.0373 0.0145 0.0184 STANDARD DEV: 31.4 0.0060 0.0014 0.0029 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 317.3 * 0.0261 0.0082 0.0126 STANDARD DEV: 50.6 0.0064 0.0013 0.0024 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 170.3 * 0.0283 0.0166 0.0156 STANDARD DEV: 14.1 0.0078 0.0043 0.0045 --------------------------------------------------------------- --------------------------------------------------------------- 281 Table 30 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL BRAIN -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 442.3 2.1728 0.4934 1.0000 STANDARD DEV: 31.8 0.0240 0.0382 0.0000 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 257.4 2.0222 0.7919 1.0000 STANDARD DEV: 31.4 0.0981 0.0687 0.0000 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 317.3 * 2.0479 0.6519 * 1.0000 STANDARD DEV: 50.6 0.1770 0.0594 0.0000 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 170.3 * 1.8176 * 1.0704 * 1.0000 STANDARD DEV: 14.1 0.1064 0.0662 0.0000 --------------------------------------------------------------- --------------------------------------------------------------- 282 Table 30 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL LEFT KIDNEY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 442.3 1.4309 0.3240 0.6586 STANDARD DEV: 31.8 0.1350 0.0269 0.0623 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 257.4 0.9220 0.3585 0.4550 STANDARD DEV: 31.4 0.1189 0.0268 0.0456 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 317.3 * 1.4077 0.4435 * 0.6856 STANDARD DEV: 50.6 0.2404 0.0220 0.0805 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 170.3 * 0.7874 * 0.4640 * 0.4338 STANDARD DEV: 14.1 0.0384 0.0309 0.0216 --------------------------------------------------------------- --------------------------------------------------------------- 283 Table 30 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL RIGHT KIDNEY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 442.3 1.4168 0.3207 0.6520 STANDARD DEV: 31.8 0.1080 0.0177 0.0489 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 257.4 0.9348 0.3637 0.4616 STANDARD DEV: 31.4 0.1123 0.0252 0.0441 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 317.3 * 1.4785 0.4640 * 0.7191 STANDARD DEV: 50.6 0.2950 0.0242 0.1033 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 170.3 * 0.7994 * 0.4710 * 0.4400 STANDARD DEV: 14.1 0.0471 0.0323 0.0132 --------------------------------------------------------------- --------------------------------------------------------------- 284 Table 30 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL LIVER -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 442.3 11.4244 2.5734 5.2578 STANDARD DEV: 31.8 1.6279 0.2217 0.7478 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 257.4 7.2921 2.8388 3.6049 STANDARD DEV: 31.4 0.7864 0.1198 0.3306 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 317.3 * 21.6104 * 6.8321 * 10.5688 * STANDARD DEV: 50.6 3.4376 0.5778 1.4711 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 170.3 * 11.7394 * 6.9106 * 6.4698 * STANDARD DEV: 14.1 0.7410 0.3939 0.4478 --------------------------------------------------------------- --------------------------------------------------------------- 285 Table 30 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL LUNG -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 442.3 1.4802 0.3350 0.6809 STANDARD DEV: 31.8 0.1217 0.0213 0.0501 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 257.4 1.2546 0.4874 0.6195 STANDARD DEV: 31.4 0.1756 0.0348 0.0726 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 317.3 * 1.2530 0.3928 * 0.6091 STANDARD DEV: 50.6 0.2690 0.0306 0.0971 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 170.3 * 0.8541 * 0.5028 0.4704 * STANDARD DEV: 14.1 0.0609 0.0340 0.0313 --------------------------------------------------------------- --------------------------------------------------------------- 286 Table 30 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL LEFT OVARY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 MEAN: 442.3 STANDARD DEV: 31.8 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 257.4 0.0663 0.0255 0.0326 STANDARD DEV: 31.4 0.0160 0.0043 0.0070 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 MEAN: 317.3 * STANDARD DEV: 50.6 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 170.3 * 0.0468 * 0.0276 0.0258 STANDARD DEV: 14.1 0.0078 0.0048 0.0048 --------------------------------------------------------------- --------------------------------------------------------------- 287 Table 30 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL RIGHT OVARY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 MEAN: 442.3 STANDARD DEV: 31.8 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 257.4 0.0733 0.0282 0.0361 STANDARD DEV: 31.4 0.0184 0.0044 0.0081 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 MEAN: 317.3 * STANDARD DEV: 50.6 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 170.3 * 0.0527 0.0311 0.0291 STANDARD DEV: 14.1 0.0085 0.0050 0.0052 --------------------------------------------------------------- --------------------------------------------------------------- 288 Table 30 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL SPLEEN -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 442.3 0.7326 0.1646 0.3372 STANDARD DEV: 31.8 0.1440 0.0248 0.0666 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 257.4 0.4972 0.1942 0.2454 STANDARD DEV: 31.4 0.0520 0.0188 0.0142 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 317.3 * 0.5901 0.1852 0.2869 STANDARD DEV: 50.6 0.1298 0.0199 0.0482 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 170.3 * 0.3599 * 0.2127 0.1981 * STANDARD DEV: 14.1 0.0407 0.0312 0.0193 --------------------------------------------------------------- --------------------------------------------------------------- 289 Table 30 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL LEFT TESTIS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 442.3 1.4941 0.3378 0.6875 STANDARD DEV: 31.8 0.1390 0.0185 0.0615 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 MEAN: 257.4 STANDARD DEV: 31.4 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 317.3 * 1.7529 * 0.5580 * 0.8581 * STANDARD DEV: 50.6 0.1520 0.0501 0.0633 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 MEAN: 170.3 * STANDARD DEV: 14.1 --------------------------------------------------------------- --------------------------------------------------------------- 290 Table 30 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL RIGHT TESTIS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 442.3 1.5159 0.3424 0.6976 STANDARD DEV: 31.8 0.1642 0.0221 0.0746 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 MEAN: 257.4 STANDARD DEV: 31.4 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 317.3 * 1.7399 0.5535 * 0.8511 * STANDARD DEV: 50.6 0.1642 0.0492 0.0601 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 MEAN: 170.3 * STANDARD DEV: 14.1 --------------------------------------------------------------- --------------------------------------------------------------- 291 Table 30 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL LEFT THYROID/PAR -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 4 4 4 MEAN: 442.3 0.0128 0.0028 0.0059 STANDARD DEV: 31.8 0.0026 0.0006 0.0012 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 257.4 0.0083 0.0032 0.0041 STANDARD DEV: 31.4 0.0021 0.0007 0.0010 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 4 4 4 MEAN: 317.3 * 0.0093 0.0031 0.0047 STANDARD DEV: 50.6 0.0015 0.0009 0.0012 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 4 4 4 MEAN: 170.3 * 0.0076 0.0043 0.0041 STANDARD DEV: 14.1 0.0022 0.0013 0.0013 --------------------------------------------------------------- --------------------------------------------------------------- 292 Table 30 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL RIGHT THYROID/PR -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 442.3 0.0113 0.0025 0.0052 STANDARD DEV: 31.8 0.0049 0.0010 0.0022 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 257.4 0.0074 0.0029 0.0037 STANDARD DEV: 31.4 0.0022 0.0008 0.0011 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 317.3 * 0.0102 0.0032 0.0050 STANDARD DEV: 50.6 0.0033 0.0009 0.0015 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 170.3 * 0.0078 0.0046 0.0043 STANDARD DEV: 14.1 0.0024 0.0016 0.0014 --------------------------------------------------------------- --------------------------------------------------------------- 293 Table 30 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL UTERUS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 MEAN: 442.3 STANDARD DEV: 31.8 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 257.4 0.5992 0.2334 0.2958 STANDARD DEV: 31.4 0.1269 0.0468 0.0584 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 MEAN: 317.3 * STANDARD DEV: 50.6 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 170.3 * 0.4823 0.2821 0.2633 STANDARD DEV: 14.1 0.1903 0.1076 0.0945 --------------------------------------------------------------- --------------------------------------------------------------- 294 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT ADRENAL -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 535.9 0.0373 0.0069 0.0168 STANDARD DEV: 54.5 0.0080 0.0008 0.0035 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 291.3 0.0336 0.0119 0.0169 STANDARD DEV: 46.9 0.0052 0.0032 0.0030 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 531.5 0.0277 0.0052 0.0127 STANDARD DEV: 24.6 0.0061 0.0013 0.0031 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 255.2 0.0360 0.0142 0.0185 STANDARD DEV: 30.7 0.0055 0.0024 0.0025 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 539.3 0.0295 0.0055 0.0129 STANDARD DEV: 70.5 0.0022 0.0007 0.0010 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 264.2 0.0369 0.0141 0.0186 STANDARD DEV: 31.1 0.0077 0.0030 0.0035 --------------------------------------------------------------- --------------------------------------------------------------- 295 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT ADRENAL -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 473.3 0.0303 0.0065 0.0139 STANDARD DEV: 39.1 0.0034 0.0011 0.0019 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 236.9 0.0322 0.0137 0.0173 STANDARD DEV: 21.2 0.0049 0.0027 0.0036 --------------------------------------------------------------- 296 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT ADRENAL -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 535.9 0.0353 0.0065 0.0159 STANDARD DEV: 54.5 0.0076 0.0009 0.0033 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 291.3 0.0337 0.0119 0.0171 STANDARD DEV: 46.9 0.0071 0.0034 0.0042 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 531.5 0.0256* 0.0048 * 0.0118 STANDARD DEV: 24.6 0.0043 0.0009 0.0023 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 255.2 0.0336 0.0132 0.0173 STANDARD DEV: 30.7 0.0052 0.0016 0.0024 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 539.3 0.0273 0.0051 * 0.0119 STANDARD DEV: 70.5 0.0034 0.0004 0.0012 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 264.2 0.0345 0.0131 0.0174 STANDARD DEV: 31.1 0.0059 0.0019 0.0025 --------------------------------------------------------------- --------------------------------------------------------------- 297 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT ADRENAL -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 473.3 0.0286 0.0061 0.0131 STANDARD DEV: 39.1 0.0030 0.0009 0.0018 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 236.9 0.0307 0.0131 0.0165 STANDARD DEV: 21.2 0.0036 0.0023 0.0028 --------------------------------------------------------------- 298 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL BRAIN -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 535.9 2.2215 0.4182 1.0000 STANDARD DEV: 54.5 0.0445 0.0452 0.0000 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 291.3 1.9905 0.6962 1.0000 STANDARD DEV: 46.9 0.0791 0.1058 0.0000 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 531.5 2.1920 0.4126 1.0000 STANDARD DEV: 24.6 0.0960 0.0123 0.0000 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 255.2 1.9423 0.7665 1.0000 STANDARD DEV: 30.7 0.1026 0.0587 0.0000 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 539.3 2.2818 0.4273 1.0000 STANDARD DEV: 70.5 0.1054 0.0430 0.0000 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 264.2 1.9846 0.7566 1.0000 STANDARD DEV: 31.1 0.1121 0.0628 0.0000 --------------------------------------------------------------- --------------------------------------------------------------- 299 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL BRAIN -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 473.3 2.1861 0.4642 1.0000 STANDARD DEV: 39.1 0.0723 0.0397 0.0000 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 236.9 1.8793 0.7961 1.0000 STANDARD DEV: 21.2 0.1322 0.0609 0.0000 --------------------------------------------------------------- 300 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT KIDNEY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 535.9 1.9711 0.3686 0.8874 STANDARD DEV: 54.5 0.1974 0.0233 0.0880 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 291.3 1.1328 0.3911 0.5688 STANDARD DEV: 46.9 0.1492 0.0283 0.0667 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 531.5 1.8955 0.3570 0.8661 STANDARD DEV: 24.6 0.1575 0.0307 0.0818 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 255.2 0.9311 0.3658 0.4782 STANDARD DEV: 30.7 0.1262 0.0386 0.0473 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 539.3 2.0805 0.3863 0.9111 STANDARD DEV: 70.5 0.2924 0.0302 0.1154 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 264.2 1.1345 0.4307 0.5718 STANDARD DEV: 31.1 0.1316 0.0362 0.0615 --------------------------------------------------------------- --------------------------------------------------------------- 301 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT KIDNEY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 473.3 2.1488 0.4535 * 0.9817 STANDARD DEV: 39.1 0.2660 0.0313 0.1017 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 236.9 0.9648 0.4053 0.5124 STANDARD DEV: 21.2 0.1536 0.0302 0.0648 --------------------------------------------------------------- 302 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT KIDNEY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 535.9 2.0585 0.3846 0.9265 STANDARD DEV: 54.5 0.2090 0.0203 0.0904 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 291.3 1.1236 0.3874 0.5644 STANDARD DEV: 46.9 0.1700 0.0341 0.0797 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 531.5 1.9405 0.3656 0.8873 STANDARD DEV: 24.6 0.1411 0.0297 0.0831 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 255.2 0.9328 0.3652 0.4787 STANDARD DEV: 30.7 0.1505 0.0354 0.0596 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 539.3 2.0643 0.3835 0.9041 STANDARD DEV: 70.5 0.2531 0.0209 0.0972 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 264.2 1.1634 0.4418 0.5865 STANDARD DEV: 31.1 0.1471 0.0451 0.0712 --------------------------------------------------------------- --------------------------------------------------------------- 303 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT KIDNEY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 473.3 2.1724 0.4581 * 0.9929 STANDARD DEV: 39.1 0.2812 0.0303 0.1136 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 236.9 0.9912 0.4166 0.5264 STANDARD DEV: 21.2 0.1534 0.0305 0.0638 --------------------------------------------------------------- 304 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LIVER -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 535.9 15.9996 2.9783 7.2008 STANDARD DEV: 54.5 2.1505 0.1425 0.9432 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 291.3 8.7487 3.0096 4.4018 STANDARD DEV: 46.9 1.2794 0.1220 0.6612 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 531.5 15.6102 2.9345 7.1245 STANDARD DEV: 24.6 1.7249 0.2531 0.7384 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 255.2 7.4501 2.9233 3.8304 STANDARD DEV: 30.7 1.0845 0.2753 0.4607 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 539.3 18.0657 3.3764 7.9217 STANDARD DEV: 70.5 1.7387 0.3717 0.7535 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 264.2 8.8944 3.3658 4.4714 STANDARD DEV: 31.1 1.3628 0.3182 0.5448 --------------------------------------------------------------- --------------------------------------------------------------- 305 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LIVER -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 473.3 24.2906 * 5.1173 * 11.1093 * STANDARD DEV: 39.1 3.5145 0.3964 1.5191 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 236.9 9.7445 4.1155 * 5.1846 STANDARD DEV: 21.2 1.1880 0.3584 0.5040 --------------------------------------------------------------- 306 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LUNG -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 535.9 1.8736 0.3510 0.8428 STANDARD DEV: 54.5 0.1592 0.0280 0.0609 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 291.3 1.2020 0.4182 0.6048 STANDARD DEV: 46.9 0.1063 0.0552 0.0590 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 531.5 1.7104 0.3226 0.7811 STANDARD DEV: 24.6 0.0972 0.0267 0.0493 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 255.2 1.1997 0.4698 0.6163 STANDARD DEV: 30.7 0.1562 0.0101 0.0571 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 539.3 1.9907 0.3701 0.8707 STANDARD DEV: 70.5 0.2684 0.0333 0.0899 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 264.2 1.1830 0.4513 0.5968 STANDARD DEV: 31.1 0.0601 0.0396 0.0305 --------------------------------------------------------------- --------------------------------------------------------------- 307 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LUNG -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 473.3 1.6290 0.3467 0.7450 STANDARD DEV: 39.1 0.1108 0.0448 0.0411 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 236.9 1.0859 0.4601 0.5784 STANDARD DEV: 21.2 0.0776 0.0363 0.0303 --------------------------------------------------------------- 308 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT OVARY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 MEAN: 535.9 STANDARD DEV: 54.5 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 291.3 0.0742 0.0263 0.0374 STANDARD DEV: 46.9 0.0125 0.0072 0.0067 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 MEAN: 531.5 STANDARD DEV: 24.6 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 255.2 0.0523 0.0206 0.0269 STANDARD DEV: 30.7 0.0097 0.0034 0.0043 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 MEAN: 539.3 STANDARD DEV: 70.5 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 264.2 0.0608 0.0230 0.0304 STANDARD DEV: 31.1 0.0201 0.0070 0.0093 --------------------------------------------------------------- --------------------------------------------------------------- 309 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT OVARY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 MEAN: 473.3 STANDARD DEV: 39.1 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 236.9 0.0514 0.0218 0.0275 STANDARD DEV: 21.2 0.0017 0.0016 0.0022 --------------------------------------------------------------- 310 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT OVARY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 MEAN: 535.9 STANDARD DEV: 54.5 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 291.3 0.0596 0.0208 0.0301 STANDARD DEV: 46.9 0.0166 0.0061 0.0090 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 MEAN: 531.5 STANDARD DEV: 24.6 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 255.2 0.0518 0.0201 0.0264 STANDARD DEV: 30.7 0.0140 0.0047 0.0063 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 MEAN: 539.3 STANDARD DEV: 70.5 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 264.2 0.0623 0.0236 0.0312 STANDARD DEV: 31.1 0.0128 0.0040 0.0054 --------------------------------------------------------------- --------------------------------------------------------------- 311 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT OVARY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 MEAN: 473.3 STANDARD DEV: 39.1 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 236.9 0.0541 0.0229 0.0289 STANDARD DEV: 21.2 0.0054 0.0026 0.0036 --------------------------------------------------------------- 312 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL SPLEEN -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 535.9 0.7252 0.1345 0.3266 STANDARD DEV: 54.5 0.1659 0.0257 0.0755 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 291.3 0.4690 0.1622 0.2360 STANDARD DEV: 46.9 0.0560 0.0152 0.0293 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 531.5 0.8193 0.1544 0.3743 STANDARD DEV: 24.6 0.0229 0.0079 0.0186 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 255.2 0.4603 0.1793 0.2358 STANDARD DEV: 30.7 0.0829 0.0145 0.0329 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 539.3 0.7152 0.1328 0.3135 STANDARD DEV: 70.5 0.1209 0.0166 0.0524 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 264.2 0.4512 0.1711 0.2272 STANDARD DEV: 31.1 0.0531 0.0124 0.0226 --------------------------------------------------------------- --------------------------------------------------------------- 313 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 20 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL SPLEEN -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 473.3 0.6639 0.1408 0.3037 STANDARD DEV: 39.1 0.0293 0.0101 0.0066 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 236.9 0.4064 0.1730 0.2170 STANDARD DEV: 21.2 0.0722 0.0356 0.0408 --------------------------------------------------------------- 314 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 21 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT TESTIS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 535.9 1.6975 0.3204 0.7645 STANDARD DEV: 54.5 0.1684 0.0570 0.0789 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 MEAN: 291.3 STANDARD DEV: 46.9 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 531.5 1.7368 0.3270 0.7924 STANDARD DEV: 24.6 0.0912 0.0161 0.0251 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 MEAN: 255.2 STANDARD DEV: 30.7 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 539.3 1.6528 0.3100 0.7254 STANDARD DEV: 70.5 0.0711 0.0367 0.0433 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 MEAN: 264.2 STANDARD DEV: 31.1 --------------------------------------------------------------- --------------------------------------------------------------- 315 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 22 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT TESTIS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 473.3 1.7151 0.3629 0.7841 STANDARD DEV: 39.1 0.1400 0.0209 0.0500 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 MEAN: 236.9 STANDARD DEV: 21.2 --------------------------------------------------------------- 316 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 23 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT TESTIS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 535.9 1.7042 0.3211 0.7677 STANDARD DEV: 54.5 0.1307 0.0473 0.0648 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 MEAN: 291.3 STANDARD DEV: 46.9 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 531.5 1.7733 0.3339 0.8087 STANDARD DEV: 24.6 0.1140 0.0204 0.0315 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 MEAN: 255.2 STANDARD DEV: 30.7 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 539.3 1.6684 0.3131 0.7324 STANDARD DEV: 70.5 0.0976 0.0402 0.0561 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 MEAN: 264.2 STANDARD DEV: 31.1 --------------------------------------------------------------- --------------------------------------------------------------- 317 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 24 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT TESTIS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 473.3 1.7278 0.3653 0.7902 STANDARD DEV: 39.1 0.1665 0.0253 0.0681 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 MEAN: 236.9 STANDARD DEV: 21.2 --------------------------------------------------------------- 318 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 25 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT THYROID/PAR -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 535.9 0.0125 0.0023 0.0056 STANDARD DEV: 54.5 0.0029 0.0005 0.0012 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 291.3 0.0105 0.0036 0.0053 STANDARD DEV: 46.9 0.0040 0.0013 0.0022 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 531.5 0.0114 0.0021 0.0052 STANDARD DEV: 24.6 0.0022 0.0004 0.0010 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 255.2 0.0076 0.0029 0.0039 STANDARD DEV: 30.7 0.0025 0.0007 0.0012 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 539.3 0.0132 0.0025 0.0058 STANDARD DEV: 70.5 0.0023 0.0006 0.0012 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 264.2 0.0093 0.0035 0.0047 STANDARD DEV: 31.1 0.0025 0.0008 0.0012 --------------------------------------------------------------- --------------------------------------------------------------- 319 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 26 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT THYROID/PAR -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 473.3 0.0144 0.0031 0.0066 STANDARD DEV: 39.1 0.0008 0.0003 0.0005 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 236.9 0.0092 0.0039 0.0049 STANDARD DEV: 21.2 0.0017 0.0005 0.0007 --------------------------------------------------------------- 320 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT THYROID/PR -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 535.9 0.0120 0.0022 0.0054 STANDARD DEV: 54.5 0.0021 0.0003 0.0009 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 291.3 0.0080 0.0029 0.0041 STANDARD DEV: 46.9 0.0026 0.0011 0.0014 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 531.5 0.0128 0.0024 0.0058 STANDARD DEV: 24.6 0.0041 0.0008 0.0017 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 255.2 0.0093 0.0037 0.0048 STANDARD DEV: 30.7 0.0033 0.0014 0.0017 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 539.3 0.0138 0.0026 0.0061 STANDARD DEV: 70.5 0.0019 0.0006 0.0010 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 264.2 0.0119 0.0045 0.0059 STANDARD DEV: 31.1 0.0042 0.0013 0.0019 --------------------------------------------------------------- --------------------------------------------------------------- 321 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 28 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT THYROID/PR -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 473.3 0.0125 0.0026 0.0057 STANDARD DEV: 39.1 0.0014 0.0002 0.0006 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 236.9 0.0092 0.0039 0.0048 STANDARD DEV: 21.2 0.0021 0.0009 0.0008 --------------------------------------------------------------- 322 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 29 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL UTERUS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 MEAN: 535.9 STANDARD DEV: 54.5 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 291.3 0.6337 0.2209 0.3197 STANDARD DEV: 46.9 0.1022 0.0448 0.0613 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 MEAN: 531.5 STANDARD DEV: 24.6 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 255.2 0.5244 0.2058 0.2695 STANDARD DEV: 30.7 0.0677 0.0151 0.0269 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 MEAN: 539.3 STANDARD DEV: 70.5 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 264.2 0.6754 0.2547 0.3404 STANDARD DEV: 31.1 0.1666 0.0519 0.0847 --------------------------------------------------------------- --------------------------------------------------------------- 323 Table 31 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 30 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL UTERUS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 MEAN: 473.3 STANDARD DEV: 39.1 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 236.9 0.5414 0.2293 0.2898 STANDARD DEV: 21.2 0.0477 0.0207 0.0368 --------------------------------------------------------------- 324 Table 32 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL LEFT ADRENAL -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 722.2 0.0350 0.0049 0.0147 STANDARD DEV: 58.0 0.0031 0.0005 0.0016 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 406.9 0.0445 0.0112 0.0202 STANDARD DEV: 32.2 0.0140 0.0043 0.0066 --------------------------------------------------------------- M4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 639.0 * 0.0335 0.0053 0.0141 STANDARD DEV: 71.5 0.0057 0.0011 0.0025 --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 306.3 * 0.0375 0.0125 0.0174 STANDARD DEV: 49.7 0.0061 0.0030 0.0035 --------------------------------------------------------------- --------------------------------------------------------------- 325 Table 32 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL RIGHT ADRENAL -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 722.2 0.0316 0.0044 0.0132 STANDARD DEV: 58.0 0.0049 0.0006 0.0016 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 406.9 0.0409 0.0103 0.0185 STANDARD DEV: 32.2 0.0146 0.0044 0.0067 --------------------------------------------------------------- M4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 639.0 * 0.0324 0.0052 0.0137 STANDARD DEV: 71.5 0.0090 0.0016 0.0039 --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 306.3 * 0.0347 0.0117 0.0161 STANDARD DEV: 49.7 0.0041 0.0025 0.0024 --------------------------------------------------------------- --------------------------------------------------------------- 326 Table 32 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL BRAIN -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 722.2 2.3837 0.3307 1.0000 STANDARD DEV: 58.0 0.1629 0.0180 0.0000 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 406.9 2.2100 0.5452 1.0000 STANDARD DEV: 32.2 0.0852 0.0395 0.0000 --------------------------------------------------------------- M4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 639.0 * 2.3804 0.3753 * 1.0000 STANDARD DEV: 71.5 0.1067 0.0300 0.0000 --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 306.3 * 2.1689 0.7201 * 1.0000 STANDARD DEV: 49.7 0.1318 0.0873 0.0000 --------------------------------------------------------------- --------------------------------------------------------------- 327 Table 32 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL LEFT KIDNEY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 722.2 2.3249 0.3224 0.9749 STANDARD DEV: 58.0 0.1910 0.0201 0.0261 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 406.9 1.3605 0.3375 0.6164 STANDARD DEV: 32.2 0.1412 0.0587 0.0694 --------------------------------------------------------------- M4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 639.0 * 2.4578 0.3856 * 1.0314 STANDARD DEV: 71.5 0.3355 0.0421 0.1225 --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 306.3 * 1.4508 0.4751 * 0.6686 STANDARD DEV: 49.7 0.2497 0.0490 0.1064 --------------------------------------------------------------- --------------------------------------------------------------- 328 Table 32 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL RIGHT KIDNEY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 722.2 2.3397 0.3247 0.9821 STANDARD DEV: 58.0 0.1416 0.0166 0.0202 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 406.9 1.4036 0.3486 0.6372 STANDARD DEV: 32.2 0.1605 0.0654 0.0898 --------------------------------------------------------------- M4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 639.0 * 2.4429 0.3827 * 1.0246 STANDARD DEV: 71.5 0.3646 0.0415 0.1316 --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 306.3 * 1.5208 0.4992 * 0.7013 STANDARD DEV: 49.7 0.2577 0.0611 0.1130 --------------------------------------------------------------- --------------------------------------------------------------- 329 Table 32 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL LIVER -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 722.2 20.8522 2.8910 8.7696 STANDARD DEV: 58.0 1.6996 0.1779 0.8214 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 406.9 11.1366 2.7565 5.0503 STANDARD DEV: 32.2 0.5964 0.3325 0.4241 --------------------------------------------------------------- M4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 639.0 * 30.8941 * 4.8476 * 12.9831 * STANDARD DEV: 71.5 5.4082 0.7573 2.2029 --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 306.3 * 17.2772 * 5.6508 * 7.9473 * STANDARD DEV: 49.7 3.6046 0.8632 1.5028 --------------------------------------------------------------- --------------------------------------------------------------- 330 Table 32 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL LUNG -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 722.2 2.1417 0.2979 0.9013 STANDARD DEV: 58.0 0.2399 0.0401 0.1183 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 406.9 1.7049 0.4211 0.7742 STANDARD DEV: 32.2 0.1511 0.0499 0.0974 --------------------------------------------------------------- M4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 639.0 * 2.2129 0.3472 * 0.9301 STANDARD DEV: 71.5 0.3222 0.0412 0.1290 --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 306.3 * 1.6432 0.5458 * 0.7592 STANDARD DEV: 49.7 0.1425 0.0770 0.0720 --------------------------------------------------------------- --------------------------------------------------------------- 331 Table 32 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL LEFT OVARY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 MEAN: 722.2 STANDARD DEV: 58.0 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 406.9 0.0646 0.0159 0.0292 STANDARD DEV: 32.2 0.0121 0.0031 0.0055 --------------------------------------------------------------- M4 ------------------------------- NUMBER IN GROUP: 10 MEAN: 639.0 * STANDARD DEV: 71.5 --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 306.3 * 0.0731 0.0245 * 0.0338 STANDARD DEV: 49.7 0.0139 0.0062 0.0067 --------------------------------------------------------------- --------------------------------------------------------------- 332 Table 32 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL RIGHT OVARY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 MEAN: 722.2 STANDARD DEV: 58.0 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 406.9 0.0607 0.0150 0.0275 STANDARD DEV: 32.2 0.0097 0.0026 0.0044 --------------------------------------------------------------- M4 ------------------------------- NUMBER IN GROUP: 10 MEAN: 639.0 * STANDARD DEV: 71.5 --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 306.3 * 0.0781 0.0262 * 0.0361 STANDARD DEV: 49.7 0.0242 0.0096 0.0114 --------------------------------------------------------------- --------------------------------------------------------------- 333 Table 32 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL SPLEEN -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 722.2 0.9636 0.1337 0.4063 STANDARD DEV: 58.0 0.1238 0.0168 0.0660 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 406.9 0.6205 0.1531 0.2808 STANDARD DEV: 32.2 0.0408 0.0135 0.0155 --------------------------------------------------------------- M4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 639.0 * 0.9192 0.1421 0.3838 STANDARD DEV: 71.5 0.3274 0.0398 0.1251 --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 306.3 * 0.5425 0.1792 0.2507 STANDARD DEV: 49.7 0.0914 0.0311 0.0442 --------------------------------------------------------------- --------------------------------------------------------------- 334 Table 32 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL LEFT TESTIS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 722.2 1.6043 0.2228 0.6747 STANDARD DEV: 58.0 0.1032 0.0164 0.0509 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 MEAN: 406.9 STANDARD DEV: 32.2 --------------------------------------------------------------- M4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 639.0 * 1.7040 0.2710 0.7196 STANDARD DEV: 71.5 0.2865 0.0609 0.1362 --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 10 MEAN: 306.3 * STANDARD DEV: 49.7 --------------------------------------------------------------- --------------------------------------------------------------- 335 Table 32 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL RIGHT TESTIS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 722.2 1.6110 0.2237 0.6772 STANDARD DEV: 58.0 0.0997 0.0145 0.0428 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 MEAN: 406.9 STANDARD DEV: 32.2 --------------------------------------------------------------- M4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 639.0 * 1.7805 * 0.2825 * 0.7506 STANDARD DEV: 71.5 0.1392 0.0442 0.0799 --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 10 MEAN: 306.3 * STANDARD DEV: 49.7 --------------------------------------------------------------- --------------------------------------------------------------- 336 Table 32 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL LEFT THYROID/PAR -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 722.2 0.0151 0.0021 0.0063 STANDARD DEV: 58.0 0.0027 0.0003 0.0011 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 406.9 0.0148 0.0037 0.0068 STANDARD DEV: 32.2 0.0045 0.0011 0.0022 --------------------------------------------------------------- M4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 639.0 * 0.0235 * 0.0037 * 0.0099 * STANDARD DEV: 71.5 0.0038 0.0004 0.0015 --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 306.3 * 0.0156 0.0052 0.0072 STANDARD DEV: 49.7 0.0053 0.0021 0.0024 --------------------------------------------------------------- --------------------------------------------------------------- 337 Table 32 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL RIGHT THYROID/PR -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 722.2 0.0189 0.0026 0.0079 STANDARD DEV: 58.0 0.0044 0.0004 0.0016 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 406.9 0.0167 0.0041 0.0076 STANDARD DEV: 32.2 0.0044 0.0008 0.0019 --------------------------------------------------------------- M4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 639.0 * 0.0250 0.0039 * 0.0105 STANDARD DEV: 71.5 0.0061 0.0006 0.0024 --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 306.3 * 0.0160 0.0054 0.0074 STANDARD DEV: 49.7 0.0064 0.0025 0.0029 --------------------------------------------------------------- --------------------------------------------------------------- 338 Table 32 Summary of Organ Weight Data Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL UTERUS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 MEAN: 722.2 STANDARD DEV: 58.0 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 406.9 1.1161 0.2806 0.5103 STANDARD DEV: 32.2 0.4939 0.1321 0.2406 --------------------------------------------------------------- M4 ------------------------------- NUMBER IN GROUP: 10 MEAN: 639.0 * STANDARD DEV: 71.5 --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 306.3 * 1.1694 0.3855 0.5408 STANDARD DEV: 49.7 0.2972 0.0989 0.1387 --------------------------------------------------------------- --------------------------------------------------------------- 339 Table 33 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 4 Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=1;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** LIVER (LI) ........................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 5 5 ** END OF LIST 340 Table 34 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ................................. NUMBER EXAMINED: 5 5 5 5 NO MACROSCOPIC LESIONS BONE MARROW SMEAR TAKEN 4041 5555 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 EYE (EY) ............................................. NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 341 Table 34 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- BRAIN (BR) ........................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 LUNG (LU) ............................................ NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 4 5 5 5 MOTTLED 1000 KIDNEY (KD) .......................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 LIVER (LI) ........................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 0 5 2 LIGHT FOCUS(I)/AREA(S) LARGE MOTTLED DIFFUSELY DARK DEPRESSED FOCUS(I)/AREA(S) 0100 0502 0001 0202 0100 342 Table 34 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- HEART (HT) ........................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 SPLEEN (SP) .......................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 THYMUS (TH) .......................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 4 RED FOCUS(I)/AREA(S) 0001 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 343 Table 34 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- THYROID (TY) ......................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 TRACHEA (TR) ......................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 4 5 5 DIFFUSELY RED 0100 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 PITUITARY (PI) ....................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 344 Table 34 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 PANCREAS (PA) ........................................ NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 3 5 4 DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) 0101 0100 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 DUODENUM (DU) ........................................ NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 345 Table 34 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- ILEUM (IL) ........................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 CECUM (CE) ........................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 4 5 5 DIFFUSELY RED 0100 COLON (CO) ........................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 RECTUM (RE) .......................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 SKIN (SK) ............................................ NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 PROSTATE (PR) ........................................ NUMBER EXAMINED: 5 5 0 0 NOT REMARKABLE: 5 5 0 0 346 Table 34 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 5 5 0 0 NOT REMARKABLE: 5 5 0 0 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 5 5 0 0 NOT REMARKABLE: 5 5 0 0 TESTIS (TE) .......................................... NUMBER EXAMINED: 5 5 0 0 NOT REMARKABLE: 5 5 0 0 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 0 5 5 NOT REMARKABLE: 0 0 5 5 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 0 5 5 NOT REMARKABLE: 0 0 5 5 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 5 5 NOT REMARKABLE: 0 0 5 4 LUMEN FILLED WITH FLUID 0001 OVARY (OV) ........................................... NUMBER EXAMINED: 0 0 5 5 NOT REMARKABLE: 0 0 5 5 347 Table 34 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 5 5 NOT REMARKABLE: 0 0 5 5 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 4 5 4 5 DIFFUSELY RED 1010 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 4 5 5 EDEMA 0100 348 Table 34 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 5 5 0 0 NOT REMARKABLE: 5 5 0 0 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 ANUS (AN) ............................................ NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 TAIL (TI) ............................................ NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 PENIS (PE) ........................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 349 Table 34 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 FOOT/FOOTPAD(S) (FP) ................................. NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 350 Table 34 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- AORTA (AO) ........................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 SUBCUTANEOUS TIS (SQ) ................................ NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 TONGUE (TO) .......................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 URETER (UE) .......................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 TESTIS/EPIDIDYM (TP) ................................. NUMBER EXAMINED: 5 5 0 0 NOT REMARKABLE: 5 5 0 0 351 Table 34 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- PINNA (PN) ........................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 THYROID/PARATHYR (TT) ................................ NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 ADRENAL (AD) ......................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 ** END OF LIST ** 352 Table 35 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NO MACROSCOPIC LESIONS BONE MARROW SMEAR TAKEN URINARY BLADDER-SMALL LUMEN 22543424 55555555 00000010 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 EYE (EY) ............................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 353 Table 35 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- BRAIN (BR) ........................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 LUNG (LU) ............................................ NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 KIDNEY (KD) .......................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 4 5 5 5 5 5 5 5 LARGE PELVIS(ES) 10000000 LIVER (LI) ........................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 4 5 5 5 5 5 5 5 MOTTLED 10000000 HEART (HT) ........................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 354 Table 35 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 SPLEEN (SP) .......................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 THYMUS (TH) .......................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 4 5 5 5 5 5 5 5 MOTTLED 10000000 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 THYROID (TY) ......................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 355 Table 35 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- PARATHYROID (PT) ..................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 TRACHEA (TR) ......................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 PITUITARY (PI) ....................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 356 Table 35 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- PANCREAS (PA) ........................................ NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 4 4 5 5 4 5 3 4 DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) 00000010 11001011 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 DUODENUM (DU) ........................................ NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 ILEUM (IL) ........................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 357 Table 35 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- CECUM (CE) ........................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 COLON (CO) ........................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 RECTUM (RE) .......................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 SKIN (SK) ............................................ NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 4 5 5 4 5 ALOPECIA-FOCAL RED FOCUS(I)/AREA(S) 00000010 00010000 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 PROSTATE (PR) ........................................ NUMBER EXAMINED: 5 5 5 5 0 0 0 0 NOT REMARKABLE: 4 5 5 5 0 0 0 0 LARGE 10000000 358 Table 35 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 5 5 5 5 0 0 0 0 NOT REMARKABLE: 4 5 5 5 0 0 0 0 LARGE 10000000 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 5 5 5 5 0 0 0 0 NOT REMARKABLE: 5 5 5 5 0 0 0 0 TESTIS (TE) .......................................... NUMBER EXAMINED: 5 5 5 5 0 0 0 0 NOT REMARKABLE: 5 5 5 5 0 0 0 0 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 0 0 0 5 5 5 5 NOT REMARKABLE: 0 0 0 0 5 5 5 5 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 0 0 0 5 5 5 5 NOT REMARKABLE: 0 0 0 0 5 5 5 5 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 0 0 5 5 5 5 NOT REMARKABLE: 0 0 0 0 5 5 4 5 LUMEN FILLED WITH FLUID 00000010 359 Table 35 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- OVARY (OV) ........................................... NUMBER EXAMINED: 0 0 0 0 5 5 5 5 NOT REMARKABLE: 0 0 0 0 4 5 5 5 CYST(S) 00001000 VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 0 0 5 5 5 5 NOT REMARKABLE: 0 0 0 0 5 5 5 5 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 4 3 5 5 5 4 4 5 DIFFUSELY RED RED FOCUS(I)/AREA(S) 12000010 00000100 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 360 Table 35 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 5 5 5 5 0 0 0 0 NOT REMARKABLE: 5 5 5 5 0 0 0 0 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 ANUS (AN) ............................................ NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 TAIL (TI) ............................................ NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 361 Table 35 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- PENIS (PE) ........................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 FOOT/FOOTPAD(S) (FP) ................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 362 Table 35 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 AORTA (AO) ........................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 SUBCUTANEOUS TIS (SQ) ................................ NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 TONGUE (TO) .......................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 URETER (UE) .......................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 363 Table 35 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- TESTIS/EPIDIDYM (TP) ................................. NUMBER EXAMINED: 5 5 5 5 0 0 0 0 NOT REMARKABLE: 5 5 5 5 0 0 0 0 PINNA (PN) ........................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 THYROID/PARATHYR (TT) ................................ NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 ADRENAL (AD) ......................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 ** END OF LIST ** 364 Table 36 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ................................. NUMBER EXAMINED: 5 10 5 10 NO MACROSCOPIC LESIONS BLOOD FILM TAKEN DISCHARGE-OCULAR MALOCCLUSION BONE MARROW SMEAR TAKEN TEETH MISSING/BROKEN/WORN 33 5 10 01 04 5 10 03 21 5 10 00 02 5 10 01 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 365 Table 36 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- EYE (EY) ............................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 BRAIN (BR) ........................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 9 COMPRESSED (HYPOTHALAMUS) 01 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 58 02 5 10 5 10 LUNG (LU) ............................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 LIGHT FOCUS(I)/AREA(S) 00 KIDNEY (KD) .......................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 58 02 5 10 5 10 LIVER (LI) ........................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 LIGHT FOCUS(I)/AREA(S) MOTTLED 00 00 5 10 49 01 10 366 Table 36 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- HEART (HT) ........................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 DIFFUSELY RED 00 SPLEEN (SP) .......................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 59 01 5 10 5 10 THYMUS (TH) .......................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 367 Table 36 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- THYROID (TY) ......................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 TRACHEA (TR) ......................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 4 10 DARK FOCUS(I)/AREA(S) 10 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 00 5 10 5 10 PITUITARY (PI) ....................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 8 MASS(ES) DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) 02 00 00 5 10 47 02 10 01 368 Table 36 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 PANCREAS (PA) ........................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 9 DARK FOCUS(I)/AREA(S) 01 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 44 16 5 10 5 10 DUODENUM (DU) ........................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 369 Table 36 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ILEUM (IL) ........................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 CECUM (CE) ........................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 COLON (CO) ........................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 RECTUM (RE) .......................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 SKIN (SK) ............................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 PROSTATE (PR) ........................................ NUMBER EXAMINED: 5 10 0 0 NOT REMARKABLE: 5 10 0 0 370 Table 36 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 5 10 0 0 NOT REMARKABLE: 5 10 0 0 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 5 10 0 0 NOT REMARKABLE: 5 10 0 0 TESTIS (TE) .......................................... NUMBER EXAMINED: 5 10 0 0 NOT REMARKABLE: 5 10 0 0 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 4 8 MASS(ES) CYST(S) 0001 0012 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 5 10 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 3 8 LUMEN FILLED WITH FLUID CYST(S) ** CONTINUED ON NEXT PAGE ** 0011 0010 371 Table 36 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 3 8 THICKENED WALL 0001 OVARY (OV) ........................................... NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 5 9 CYST(S) 0001 VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 5 10 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 4 9 DIFFUSELY RED 11 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 4 10 10 5 10 5 10 372 Table 36 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 MASS(ES) 00 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 59 01 5 10 5 10 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 5 10 0 0 NOT REMARKABLE: 5 10 0 0 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 ANUS (AN) ............................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 373 Table 36 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- TAIL (TI) ............................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 59 RAISED AREA(S) 0001 PENIS (PE) ........................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 FOOT/FOOTPAD(S) (FP) ................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 374 Table 36 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 AORTA (AO) ........................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 SUBCUTANEOUS TIS (SQ) ................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 TONGUE (TO) .......................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 URETER (UE) .......................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 375 Table 36 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 TESTIS/EPIDIDYM (TP) ................................. NUMBER EXAMINED: 5 10 0 0 NOT REMARKABLE: 5 10 0 0 PINNA (PN) ........................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 THYROID/PARATHYR (TT) ................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 376 Table 36 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ADRENAL (AD) ......................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 ** END OF LIST ** 377 Table 37 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ................................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NO MACROSCOPIC LESIONS STAINS-PERINEUM/PERIANAL BLOOD FILM TAKEN DISCHARGE-OCULAR DISCHARGE-NASAL MALOCCLUSION STAINS - SKIN/HAIRCOAT ANIMAL THIN GI TRACT-DARK FLUID CONTENT BONE MARROW SMEAR TAKEN TEETH MISSING/BROKEN/WORN ANIMAL OBESE FLUID-ABDOMINAL CAVITY 0010000020 0301110100 14 22 22 17 10 13 12 15 19 12 2023101110 0100000001 1327402242 1110100010 2000000011 0000000100 14 22 22 17 10 13 13 15 17 12 1102010101 3672201101 0000000010 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 378 Table 37 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 EYE (EY) ............................................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 21 22 17 10 13 13 15 18 12 OPAQUE CORNEA RED INTRAOCULAR MATERIAL 1100000000 0000000010 BRAIN (BR) ........................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 12 18 19 14 6 7 7 7 11 6 COMPRESSED (HYPOTHALAMUS) 2433466886 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 LUNG (LU) ............................................ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 21 16 9 13 13 15 17 10 LIGHT FOCUS(I)/AREA(S) ADHESION(S) 0001100022 0010000000 379 Table 37 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- KIDNEY (KD) .......................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 17 18 13 7 11 12 15 17 10 LARGE PELVIS(ES) ROUGH SURFACE, DIFFUSE DIFFUSELY LIGHT CYST(S) DIFFUSELY DARK GRANULAR MATERIAL LARGE MASS(ES) DARK FOCUS(I)/AREA(S) SMALL 0141210001 1311010000 1301000010 1300000020 0002000000 0010001000 1200000000 0000100001 0100000000 0010000000 LIVER (LI) ........................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 6 7 10 4 5 8 7 10 4 4 LIGHT FOCUS(I)/AREA(S) LARGE MOTTLED DIFFUSELY DARK DIFFUSELY LIGHT MASS(ES) RED FOCUS(I)/AREA(S) DARK FOCUS(I)/AREA(S) CYST(S) ADHESION(S) 0212102124 0103000010 0245110131 0001000040 0010001000 1103001030 8 11 9 3 3 3 3 5 7 5 0002001020 1000000000 0000010000 380 Table 37 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 6 7 10 4 5 8 7 10 4 4 MASS #2 0000000011 HEART (HT) ........................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 21 22 17 10 13 13 15 19 12 LIGHT FOCUS(I)/AREA(S) 0100000000 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 21 22 17 10 13 13 15 19 12 ATROPHY-FOCAL 0100000000 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 21 20 15 10 11 13 12 18 11 LARGE MOTTLED DIFFUSELY RED DIFFUSELY DARK RED FOCUS(I)/AREA(S) 1101010200 0011000000 1110000110 0000010000 0000000100 381 Table 37 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 21 20 15 10 11 13 12 18 11 GELATINOUS 0000000001 SPLEEN (SP) .......................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 21 22 17 10 13 13 15 19 12 LARGE 1100000000 THYMUS (TH) .......................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 18 12 CYST(S) 0000000010 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 THYROID (TY) ......................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 19 15 9 12 12 13 19 12 LARGE DIFFUSELY DARK 0032111200 0000010000 382 Table 37 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- PARATHYROID (PT) ..................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 21 22 16 10 13 13 15 19 12 LARGE 1101000000 TRACHEA (TR) ......................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 12 20 19 16 9 7 9 11 17 10 MASS(ES) DARK FOCUS(I)/AREA(S) LARGE CYST(S) DIFFUSELY DARK SMALL RED FOCUS(I)/AREA(S) RAISED AREA(S) 0010012011 1100000000 1120031410 0000001000 0001020000 0000000001 0000000001 0000100000 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 383 Table 37 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- PITUITARY (PI) ....................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 8 17 12 8 4 5 2 1 4 2 MASS(ES) DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) LARGE RAISED AREA(S) LIGHT FOCUS(I)/AREA(S) CYST(S) 2333466786 3012112101 0110102322 0211011562 0021001000 1012000000 0010000000 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 PANCREAS (PA) ........................................ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 11 21 21 17 9 13 12 15 19 12 LARGE VESSELS MASS(ES) 3000100000 0110001000 384 Table 37 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 19 21 12 9 11 13 15 16 7 DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) THICKENED WALL EROSION/ULCERATION LIGHT FOCUS(I)/AREA(S) 0002110000 0001010011 1301000001 0012000012 0000000011 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 21 21 17 10 12 13 15 19 12 EROSION/ULCERATION THICKENED WALL 0010010000 0110010000 DUODENUM (DU) ........................................ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 12 15 19 12 LARGE LYMPHOID AREA(S) 0000001000 385 Table 37 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ILEUM (IL) ........................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 12 15 19 12 LARGE LYMPHOID AREA(S) 0000001000 CECUM (CE) ........................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 21 17 10 13 13 15 19 12 THICKENED WALL 0010000000 COLON (CO) ........................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 RECTUM (RE) .......................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 SKIN (SK) ............................................ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 12 16 17 15 8 12 12 15 18 12 MASS(ES) CRUSTED AREA(S) RAISED AREA(S) ABRASION(S)/ULCERATION(S) 0221101010 0230110000 2101000000 0110000000 386 Table 37 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 21 17 10 13 13 15 19 12 CALCULUS(I) 0010000000 PROSTATE (PR) ........................................ NUMBER EXAMINED: 14 22 22 17 10 0 0 0 0 0 NOT REMARKABLE: 11 19 17 16 9 0 0 0 0 0 NODULAR 3351100000 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 14 22 22 17 10 0 0 0 0 0 NOT REMARKABLE: 12 22 22 17 10 0 0 0 0 0 SMALL LARGE 1000000000 1000000000 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 14 22 22 17 10 0 0 0 0 0 NOT REMARKABLE: 13 22 22 17 10 0 0 0 0 0 SMALL 1000000000 TESTIS (TE) .......................................... NUMBER EXAMINED: 14 22 22 17 10 0 0 0 0 0 NOT REMARKABLE: 12 21 19 12 10 0 0 0 0 0 SMALL LARGE RED/YELLOW FOCUS(I)/AREA(S) 2124000000 0010000000 0010000000 387 Table 37 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** TESTIS (TE) .......................................... NUMBER EXAMINED: 14 22 22 17 10 0 0 0 0 0 NOT REMARKABLE: 12 21 19 12 10 0 0 0 0 0 RED FOCUS(I)/AREA(S) 0001000000 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 0 0 0 0 13 13 15 19 12 NOT REMARKABLE: 0 0 0 0 0 3 3 4 11 8 MASS(ES) CYST(S) MASS #2 THICKENED MASS #3 MASS #4 MASS #5 0000088983 0000030003 0000024642 0000054642 0000022120 0000001100 0000000100 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 0 0 0 0 13 13 15 19 12 NOT REMARKABLE: 0 0 0 0 0 11 11 14 16 10 MASS(ES) LARGE LIGHT FOCUS(I)/AREA(S) 0000001112 0000021010 0000000010 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 0 0 0 13 13 15 19 12 NOT REMARKABLE: 0 0 0 0 0 6 9 10 11 8 LUMEN FILLED WITH FLUID 0000010020 388 Table 37 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 0 0 0 13 13 15 19 12 NOT REMARKABLE: 0 0 0 0 0 6 9 10 11 8 CYST(S) THICKENED WALL LARGE, DIFFUSE MASS(ES) LARGE, FOCAL RED FOCUS(I)/AREA(S) SEMISOLID MATERIAL 0000043434 0000000020 0000020000 0000012120 0000000010 0000000100 0000000100 OVARY (OV) ........................................... NUMBER EXAMINED: 0 0 0 0 0 13 13 15 19 12 NOT REMARKABLE: 0 0 0 0 0 10 8 11 11 8 CYST(S) 0000035484 VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 0 0 0 13 13 15 19 12 NOT REMARKABLE: 0 0 0 0 0 12 13 14 19 11 MASS(ES) SEMIFLUID MATERIAL 0000000001 0000010100 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 21 21 17 10 13 12 15 18 12 LARGE DIFFUSELY RED 0010001010 1000001000 389 Table 37 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 21 21 17 10 13 12 15 18 12 CYST(S) 0100000000 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 11 16 19 16 8 11 11 11 17 7 DIFFUSELY RED LARGE MOTTLED 1500111313 1100200000 1031011112 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 20 21 17 10 13 12 15 19 12 LARGE DIFFUSELY RED 0100000000 0210001000 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 14 19 11 MASS(ES) 0000000101 390 Table 37 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 14 22 22 17 10 0 0 0 0 0 NOT REMARKABLE: 12 21 22 14 9 0 0 0 0 0 MASS(ES) CYST(S) 2103000000 0000100000 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 12 18 19 17 10 12 13 15 19 12 LARGE MOTTLED DIFFUSELY RED CYSTS 2330000000 0000010000 0010000000 0100000000 ANUS (AN) ............................................ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 21 17 10 12 12 15 18 11 LARGE DIFFUSELY RED 0000011000 0010011011 391 Table 37 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LN, RENAL (RL) ....................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 21 17 10 12 12 15 18 11 SMALL 0000010000 TAIL (TI) ............................................ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 21 22 16 10 13 13 15 19 11 CRUSTED AREA(S) LARGE 0100000001 0001000000 PENIS (PE) ........................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 18 12 DIFFUSELY DARK 0000000010 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 392 Table 37 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- FOOT/FOOTPAD(S) (FP) ................................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 11 17 16 15 6 10 13 15 18 10 CRUSTED AREA(S) ABRASION(S)/ULCERATION(S) 0422230012 3140200000 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 20 22 17 10 11 12 14 15 11 LARGE MOTTLED DIFFUSELY RED 0200011020 0100010121 0000000010 LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 21 22 17 10 12 13 15 19 12 MASS(ES) 0100010000 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 21 21 17 10 12 13 14 19 12 LARGE 0110010100 393 Table 37 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- AORTA (AO) ........................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 22 22 17 10 13 13 15 19 12 LARGE 1000000000 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 20 22 17 10 13 13 14 19 12 LARGE 1200000100 SUBCUTANEOUS TIS (SQ) ................................ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 TONGUE (TO) .......................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 URETER (UE) .......................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 394 Table 37 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- TESTIS/EPIDIDYM (TP) ................................. NUMBER EXAMINED: 14 22 22 17 10 0 0 0 0 0 NOT REMARKABLE: 14 22 22 17 10 0 0 0 0 0 PINNA (PN) ........................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 18 12 RED FOCUS(I)/AREA(S) 0000000010 THYROID/PARATHYR (TT) ................................ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 18 12 MISSING/NOT IDENTIFIED 0000000010 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 22 22 17 10 13 13 15 19 12 MASS(ES) 1000000000 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 21 22 17 10 13 13 15 19 12 LARGE 0100000000 395 Table 37 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 9 13 13 15 19 12 MASS(ES) 0000100000 ADRENAL (AD) ......................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 ** END OF LIST 396 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ................................. NUMBER EXAMINED: 36 28 28 33 3 30 1 NO MACROSCOPIC LESIONS DISCHARGE-ORAL STAINS-PERINEUM/PERIANAL BLOOD FILM TAKEN DISCHARGE-OCULAR DISCHARGE-NASAL MALOCCLUSION STAINS - SKIN/HAIRCOAT ANIMAL THIN GI TRACT-DARK FLUID CONTENT GI TRACT-LARGE LUMEN GI TRACT-DARK DRY CONTENT FLUID-THORACIC CAVITY TEETH MISSING/BROKEN/WORN AUTOLYSIS-SEVERE ANIMAL OBESE URINARY BLADDER-LARGE LUMEN YELLOWISH MUCOUS MEMBRANES TAIL - BENT/MISSING 0010000 2213211 10 4 8 10 1 7 1 24 16 23 25 1 18 0 13 6 7 10 1 6 0 4 2 5 10 2 5 0 2247230 6288070 10 6 7 9 0 8 0 0000100 1111020 0000010 1011010 0103010 0011000 5505040 3002020 0001000 0001000 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 397 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 EYE (EY) ............................................. NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 33 25 27 32 3 30 1 OPAQUE CORNEA WHITE INTRAOCULAR MATERIAL COLLAPSED GLOBE-ANTE MORTEM 2211000 1000000 0110000 BRAIN (BR) ........................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 11 12 9 9 3 12 1 COMPRESSED (HYPOTHALAMUS) 25 16 19 24 0 18 0 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 398 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LUNG (LU) ............................................ NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 30 23 27 30 1 23 1 DIFFUSELY RED MOTTLED UNCOLLAPSED LIGHT FOCUS(I)/AREA(S) ADHESION(S) RED FOCUS(I)/AREA(S) MASS(ES) 0201210 0011010 1210020 2001020 1102000 1000020 1002000 KIDNEY (KD) .......................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 25 21 24 23 3 23 1 LARGE PELVIS(ES) ROUGH SURFACE, DIFFUSE MOTTLED SEMIFLUID MATERIAL-PELVIS EROSION(S)/ULCERATION(S) DIFFUSELY LIGHT CYST(S) DIFFUSELY DARK CALCULUS(I) GRANULAR MATERIAL LARGE ADHESION(S) MASS(ES) ** CONTINUED ON NEXT PAGE ** 8337030 4005020 1111000 0011000 0000010 3002010 2000000 0000010 1210010 1002000 1300010 1000000 0101000 399 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** KIDNEY (KD) .......................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 25 21 24 23 3 23 1 DEPRESSED FOCUS(I)/AREA(S) 1000000 LIVER (LI) ........................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 27 20 18 20 1 23 1 LIGHT FOCUS(I)/AREA(S) LARGE MOTTLED DIFFUSELY DARK DIFFUSELY LIGHT MASS(ES) RED FOCUS(I)/AREA(S) ROUGH SURFACE, DIFFUSE DARK FOCUS(I)/AREA(S) CYST(S) 0213030 1311210 4151200 0004010 1101010 1110000 3234020 0000010 0010000 0001000 HEART (HT) ........................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 32 3 30 1 LARGE MOTTLED 0001000 0001000 400 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 35 28 27 33 3 29 1 MASS(ES) DIFFUSELY RED 1000010 0010000 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 33 28 28 32 3 30 1 LARGE MOTTLED DIFFUSELY RED DIFFUSELY DARK SMALL 1000000 1000000 0001000 1000000 1000000 SPLEEN (SP) .......................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 35 27 28 30 3 30 1 SMALL LARGE ROUGH SURFACE CYST(S) 0001000 1001000 0100000 0001000 401 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- THYMUS (TH) .......................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 34 27 28 30 2 29 1 RED FOCUS(I)/AREA(S) MASS(ES) DIFFUSELY RED MISSING/NOT IDENTIFIED 0100110 1001000 0001000 1001000 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 THYROID (TY) ......................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 27 33 3 29 1 LARGE RED FOCUS(I)/AREA(S) 0000010 0010000 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 32 3 29 1 LARGE 0001010 TRACHEA (TR) ......................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 402 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 33 27 25 31 3 30 1 MASS(ES) LARGE SMALL MISSING/NOT IDENTIFIED MOTTLED 2000000 0110000 0021000 0001000 1000000 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 PITUITARY (PI) ....................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 8 10 7 9 3 8 1 MASS(ES) RED FOCUS(I)/AREA(S) LARGE DIFFUSELY RED 28 16 19 24 0 18 0 0110020 0100020 0010000 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 403 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- PANCREAS (PA) ........................................ NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 31 3 29 1 MASS(ES) 0002010 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 25 26 24 24 2 24 0 DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) THICKENED WALL EROSION/ULCERATION MASS(ES) 7225151 2001000 3003000 2011010 0010000 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 30 24 26 26 3 25 1 LIGHT FOCUS(I)/AREA(S) EROSION/ULCERATION THICKENED WALL DARK FOCUS(I)/AREA(S) 0001000 3225040 3202030 1100010 DUODENUM (DU) ........................................ NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 404 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- JEJUNUM (JE) ......................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 ILEUM (IL) ........................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 CECUM (CE) ........................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 35 28 28 33 3 30 1 RED FOCUS(I)/AREA(S) 1000000 COLON (CO) ........................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 RECTUM (RE) .......................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 SKIN (SK) ............................................ NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 35 25 27 32 3 27 1 MASS(ES) CRUSTED AREA(S) RAISED AREA(S) 0201020 0110010 1000000 405 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 34 27 28 29 3 28 1 THICKENED WALL CALCULUS(I) SEMIFLUID MATERIAL RED FOCUS(I)/AREA(S) DIFFUSELY RED 1102000 2002010 0002000 0001000 0001020 PROSTATE (PR) ........................................ NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 30 26 22 24 3 24 1 LARGE NODULAR DIFFUSELY RED SMALL MOTTLED GELATINOUS MASS(ES) 0002000 6264040 0001000 0002010 0000010 0001000 0001000 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 27 25 24 25 3 17 1 SMALL GELATINOUS LARGE MOTTLED ** CONTINUED ON NEXT PAGE ** 5234090 1101020 3014030 1010010 406 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 27 25 24 25 3 17 1 DIFFUSELY RED 0001000 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 35 28 28 33 3 29 1 SMALL 1000010 TESTIS (TE) .......................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 32 26 25 29 3 24 1 SMALL CONTAINS FLUID DIFFUSELY RED LARGE RED/YELLOW FOCUS(I)/AREA(S) 4223060 0010000 0001000 1000000 1000000 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 407 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 OVARY (OV) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 35 28 27 32 3 30 1 LARGE DIFFUSELY RED 1001000 1011000 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 33 27 28 31 3 28 1 DIFFUSELY RED LARGE 2002010 1100010 408 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 27 28 33 3 30 1 FRACTURE(S) 0100000 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 35 27 28 32 3 30 1 LARGE MOTTLED DIFFUSELY RED 1100000 1000000 0001000 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 27 33 3 29 1 MASS(ES) 0010010 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 28 24 26 30 3 28 1 MASS(ES) CYST(S) THICKENED 2202010 5210010 1011000 409 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 33 23 27 29 3 30 1 LARGE MOTTLED DIFFUSELY RED 3414000 2100000 0100000 ANUS (AN) ............................................ NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 29 1 MASS(ES) 0000010 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 32 28 28 32 3 29 1 LARGE MOTTLED CYST(S) 4000010 1000000 0001000 TAIL (TI) ............................................ NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 34 25 27 32 3 27 1 RAISED AREA(S) CRUSTED AREA(S) ABRASION/ULCERATIONS DIFFUSELY DARK 1100010 1111010 0100000 0000010 410 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- PENIS (PE) ........................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 35 27 28 33 3 30 1 PROTRUDING 1100000 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 FOOT/FOOTPAD(S) (FP) ................................. NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 27 26 22 28 3 24 1 CRUSTED AREA(S) ABRASION(S)/ULCERATION(S) 5232050 4033020 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 31 3 30 1 DIFFUSELY RED ** CONTINUED ON NEXT PAGE ** 0002000 411 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 31 3 30 1 LARGE 0001000 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 32 3 30 1 THICKENED 0001000 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 35 28 28 32 3 30 1 LARGE 1001000 AORTA (AO) ........................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 34 28 28 33 3 30 1 LARGE THICKENED WALL 2000000 1000000 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 27 33 3 30 1 LARGE 0010000 412 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- SUBCUTANEOUS TIS (SQ) ................................ NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 TONGUE (TO) .......................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 URETER (UE) .......................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 28 1 LARGE 0000020 AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 35 28 28 33 3 30 1 LARGE 1000000 TESTIS/EPIDIDYM (TP) ................................. NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 PINNA (PN) ........................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 29 1 CRUSTED AREA(S) 0000010 413 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- THYROID/PARATHYR (TT) ................................ NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 ADRENAL (AD) ......................................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 33 3 30 1 ** END OF LIST ** 414 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ................................. NUMBER EXAMINED: 37 37 35 30 0 28 0 DISCHARGE-ORAL STAINS-PERINEUM/PERIANAL BLOOD FILM TAKEN DISCHARGE-OCULAR DISCHARGE-NASAL MALOCCLUSION STAINS - SKIN/HAIRCOAT ANIMAL THIN GI TRACT-DARK FLUID CONTENT GI TRACT-LARGE LUMEN GI TRACT-DARK DRY CONTENT FLUID-THORACIC CAVITY TEETH MISSING/BROKEN/WORN AUTOLYSIS-SEVERE ANIMAL OBESE TAIL - BENT/MISSING 0112010 7 7 14 12 0 5 0 31 31 29 24 0 22 0 18 19 19 12 0 11 0 3 5 5 11 0 3 0 2856050 3429050 11 10 18 12 0 9 0 0100000 0000010 0010010 0010020 1101010 0010000 1110010 0000010 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 415 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 20 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 EYE (EY) ............................................. NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 34 30 0 26 0 OPAQUE CORNEA WHITE INTRAOCULAR MATERIAL 0010010 0000010 BRAIN (BR) ........................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 5 5 6 8 0 10 0 COMPRESSED (HYPOTHALAMUS) 32 32 29 22 0 18 0 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 LUNG (LU) ............................................ NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 36 35 34 26 0 24 0 DIFFUSELY RED ** CONTINUED ON NEXT PAGE ** 1110000 416 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 21 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LUNG (LU) ............................................ NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 36 35 34 26 0 24 0 UNCOLLAPSED LIGHT FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) MASS(ES) 0111000 0003020 0000010 0000010 KIDNEY (KD) .......................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 30 35 31 25 0 25 0 LARGE PELVIS(ES) ROUGH SURFACE, DIFFUSE MOTTLED DIFFUSELY LIGHT CYST(S) DIFFUSELY DARK CALCULUS(I) GRANULAR MATERIAL LIGHT FOCUS(I)/AREA(S) 4130010 0000010 1101000 0000010 1002000 0002000 1000000 3010010 0000010 LIVER (LI) ........................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 28 27 32 17 0 19 0 LIGHT FOCUS(I)/AREA(S) LARGE MOTTLED ** CONTINUED ON NEXT PAGE ** 0113020 1102000 1205030 417 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 22 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 28 27 32 17 0 19 0 DIFFUSELY DARK DEPRESSED FOCUS(I)/AREA(S) DIFFUSELY LIGHT RED FOCUS(I)/AREA(S) DARK FOCUS(I)/AREA(S) RAISED FOCUS(I)/AREA(S) CYST(S) 0003010 0100000 4101020 4511020 0001010 0010000 0001000 HEART (HT) ........................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 29 0 28 0 DIFFUSELY LIGHT 0001000 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 418 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 23 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 35 36 32 29 0 28 0 LARGE MOTTLED DIFFUSELY RED DIFFUSELY DARK 0100000 1011000 1010000 0010000 SPLEEN (SP) .......................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 36 37 34 27 0 26 0 SMALL LARGE LIGHT FOCUS(I)/AREA(S) 1000010 0012010 0001000 THYMUS (TH) .......................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 36 36 35 30 0 28 0 MOTTLED MISSING/NOT IDENTIFIED LARGE 1000000 0100000 1000000 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 419 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 24 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- THYROID (TY) ......................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 36 36 35 29 0 28 0 DIFFUSELY RED MASS(ES) LARGE 0100000 1000000 0001000 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 29 0 28 0 LARGE 0001000 TRACHEA (TR) ......................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 30 27 27 27 0 24 0 DIFFUSELY RED DARK FOCUS(I)/AREA(S) LARGE CYST(S) LIGHT FOCUS(I)/AREA(S) DIFFUSELY DARK SMALL RED FOCUS(I)/AREA(S) ** CONTINUED ON NEXT PAGE ** 1210000 0010010 5741030 0010000 0112000 1001000 0110000 2100000 420 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 25 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 30 27 27 27 0 24 0 DIFFUSELY LIGHT 0010000 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 PITUITARY (PI) ....................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 4 4 1 6 0 5 0 MASS(ES) DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) LARGE 32 30 29 22 0 18 0 1032010 0110020 1430030 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 421 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 26 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- PANCREAS (PA) ........................................ NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 36 35 30 0 28 0 LARGE VESSELS 0100000 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 34 32 32 24 0 27 0 DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) THICKENED WALL EROSION/ULCERATION LIGHT FOCUS(I)/AREA(S) 1225010 0200000 0200000 1011000 1000000 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 31 32 34 26 0 23 0 LIGHT FOCUS(I)/AREA(S) EROSION/ULCERATION THICKENED WALL DARK FOCUS(I)/AREA(S) RAISED AREA(S) 3000000 1313040 0201020 1101010 1000000 DUODENUM (DU) ........................................ NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 422 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- JEJUNUM (JE) ......................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 36 35 35 30 0 27 0 MASS(ES) DIVERTICULUM(A) 0000010 1200000 ILEUM (IL) ........................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 CECUM (CE) ........................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 36 37 35 30 0 28 0 SMALL 1000000 COLON (CO) ........................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 RECTUM (RE) .......................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 SKIN (SK) ............................................ NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 35 34 29 0 26 0 ALOPECIA-FOCAL ** CONTINUED ON NEXT PAGE ** 0001000 423 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 28 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SKIN (SK) ............................................ NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 35 34 29 0 26 0 MASS(ES) CRUSTED AREA(S) ALOPECIA-DIFFUSE 0110010 0100010 0000010 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 PROSTATE (PR) ........................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 TESTIS (TE) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 424 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 29 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 13 12 14 16 0 11 0 MASS(ES) CYST(S) MASS #2 THICKENED MASS #3 MASS #4 MASS #5 MASS #6 20 19 17 11 0 14 0 8851010 4695020 10 11 9 4 0 4 0 1233000 0133000 0011000 0001000 CERVIX (CV) .......................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 35 35 33 29 0 27 0 MASS(ES) LARGE FIRM 0110010 2111000 0010000 UTERUS (UT) .......................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 29 31 31 22 0 25 0 LUMEN FILLED WITH FLUID CYST(S) THICKENED WALL LARGE, DIFFUSE MASS(ES) ** CONTINUED ON NEXT PAGE ** 1015010 5622010 1011000 1002010 2000010 425 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 30 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** UTERUS (UT) .......................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 29 31 31 22 0 25 0 SEMIFLUID MATERIAL 0001000 OVARY (OV) ........................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 34 31 28 18 0 22 0 CYST(S) MISSING/NOT IDENTIFIED MASS(ES) MASS #2 3 5 7 10 0 6 0 0101000 0001000 0001000 VAGINA (VA) .......................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 35 32 31 27 0 22 0 MASS(ES) SEMIFLUID MATERIAL 0020010 2523050 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 27 0 DIFFUSELY RED 0000010 426 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 31 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 33 34 31 25 0 26 0 DIFFUSELY RED LARGE MOTTLED MISSING/NOT IDENTIFIED 3112010 1220020 0012000 0001000 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 34 35 29 0 27 0 MASS(ES) 0301010 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 36 35 30 0 28 0 DEPRESSED AREA(S) 0100000 427 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 32 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 36 36 34 30 0 25 0 LARGE MOTTLED 1010030 1100000 ANUS (AN) ............................................ NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 27 0 LARGE 0000010 TAIL (TI) ............................................ NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 34 28 0 28 0 CRUSTED AREA(S) 0012000 PENIS (PE) ........................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 428 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 33 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 36 36 35 29 0 27 0 LARGE DIFFUSELY RED DIFFUSELY DARK 1101010 1100000 0001000 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 34 30 0 28 0 MASS(ES) 0010000 FOOT/FOOTPAD(S) (FP) ................................. NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 33 34 32 30 0 26 0 CRUSTED AREA(S) ABRASION(S)/ULCERATION(S) 3030020 1300000 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 28 31 31 28 0 27 0 LARGE MOTTLED CYST(S) DIFFUSELY RED 4031010 4511000 1000000 1201000 429 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 34 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 35 37 35 30 0 28 0 LARGE 2000000 AORTA (AO) ........................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 27 0 FIRM 0000010 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 SUBCUTANEOUS TIS (SQ) ................................ NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 34 30 0 28 0 MASS(ES) 0010000 430 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 35 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- TONGUE (TO) .......................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 27 0 MASS(ES) 0000010 URETER (UE) .......................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 TESTIS/EPIDIDYM (TP) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 PINNA (PN) ........................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 THYROID/PARATHYR (TT) ................................ NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 431 Table 38 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 36 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- BILE DUCT (BD) ....................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 ADRENAL (AD) ......................................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 37 35 30 0 28 0 ** END OF LIST ** 432 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ................................. NUMBER EXAMINED: 70 60 60 70 13 40 1 NO MACROSCOPIC LESIONS DISCHARGE-ORAL STAINS-PERINEUM/PERIANAL BLOOD FILM TAKEN DISCHARGE-OCULAR DISCHARGE-NASAL MALOCCLUSION STAINS - SKIN/HAIRCOAT ANIMAL THIN GI TRACT-DARK FLUID CONTENT BONE MARROW SMEAR TAKEN GI TRACT-LARGE LUMEN GI TRACT-DARK DRY CONTENT FLUID-THORACIC CAVITY TEETH MISSING/BROKEN/WORN AUTOLYSIS-SEVERE ANIMAL OBESE URINARY BLADDER-LARGE LUMEN YELLOWISH MUCOUS MEMBRANES TAIL - BENT/MISSING 14 7 12 12 5 0 0 2213211 10 7 8 11 1 8 1 43 38 45 52 1 28 0 15 6 9 14 1 7 0 4 3 5 10 2 5 0 3 5 6 18 2 7 0 7398080 12 6 7 9 0 8 0 0000100 29 27 27 32 5 10 0 1111020 0000010 1011010 1208010 0011000 8 11 7 7 0 6 0 3002020 0001000 0001000 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 433 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 EYE (EY) ............................................. NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 66 56 59 69 13 40 1 OPAQUE CORNEA WHITE INTRAOCULAR MATERIAL COLLAPSED GLOBE-ANTE MORTEM 3311000 1000000 0110000 BRAIN (BR) ........................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 43 40 38 42 13 18 1 COMPRESSED (HYPOTHALAMUS) 27 20 22 28 0 22 0 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 434 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LUNG (LU) ............................................ NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 63 55 58 66 2 32 1 DIFFUSELY RED MOTTLED UNCOLLAPSED LIGHT FOCUS(I)/AREA(S) ADHESION(S) RED FOCUS(I)/AREA(S) MASS(ES) 0201210 1011010 1210020 2002030 1112000 1000020 1002000 KIDNEY (KD) .......................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 57 48 52 56 13 30 1 LARGE PELVIS(ES) ROUGH SURFACE, DIFFUSE MOTTLED SEMIFLUID MATERIAL-PELVIS EROSION(S)/ULCERATION(S) DIFFUSELY LIGHT CYST(S) DIFFUSELY DARK CALCULUS(I) GRANULAR MATERIAL LARGE ADHESION(S) MASS(ES) ** CONTINUED ON NEXT PAGE ** 9478050 5316020 1111000 0011000 0000010 4303010 3300000 0002010 1210010 1012000 2500010 1000000 0101010 435 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** KIDNEY (KD) .......................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 57 48 52 56 13 30 1 DEPRESSED FOCUS(I)/AREA(S) DARK FOCUS(I)/AREA(S) SMALL 1000000 0100000 0010000 LIVER (LI) ........................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 52 37 38 44 7 28 1 LIGHT FOCUS(I)/AREA(S) LARGE MOTTLED DIFFUSELY DARK DEPRESSED FOCUS(I)/AREA(S) DIFFUSELY LIGHT MASS(ES) RED FOCUS(I)/AREA(S) ROUGH SURFACE, DIFFUSE DARK FOCUS(I)/AREA(S) CYST(S) 0425140 1414710 5396210 0005210 0000100 1111010 2213000 11 13 12 7 0 5 0 0000010 0012000 1001000 HEART (HT) ........................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 59 60 69 13 40 1 LARGE MOTTLED ** CONTINUED ON NEXT PAGE ** 0001000 0001000 436 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** HEART (HT) ........................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 59 60 69 13 40 1 LIGHT FOCUS(I)/AREA(S) 0100000 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 69 59 59 70 13 39 1 MASS(ES) DIFFUSELY RED ATROPHY-FOCAL 1000010 0010000 0100000 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 66 59 58 67 13 40 1 LARGE MOTTLED DIFFUSELY RED DIFFUSELY DARK SMALL 2101000 1011000 1111000 1000000 1000000 437 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- SPLEEN (SP) .......................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 68 58 60 67 13 40 1 SMALL LARGE ROUGH SURFACE CYST(S) 0001000 2101000 0100000 0001000 THYMUS (TH) .......................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 67 59 60 67 1 39 1 RED FOCUS(I)/AREA(S) MOTTLED MASS(ES) DIFFUSELY RED MISSING/NOT IDENTIFIED 0100110 1000000 1001000 0001000 1001000 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 THYROID (TY) ......................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 56 68 13 38 1 LARGE RED FOCUS(I)/AREA(S) 0032020 0010000 438 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- PARATHYROID (PT) ..................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 69 59 60 68 13 39 1 LARGE 1102010 TRACHEA (TR) ......................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 64 57 54 67 1 39 1 DIFFUSELY RED MASS(ES) DARK FOCUS(I)/AREA(S) LARGE DIFFUSELY DARK SMALL MISSING/NOT IDENTIFIED MOTTLED RAISED AREA(S) 0000100 2010000 2100000 1230000 0001000 0021000 0001000 1000000 0000010 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 439 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- PITUITARY (PI) ....................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 36 37 29 35 13 12 1 MASS(ES) DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) LARGE DIFFUSELY RED RAISED AREA(S) LIGHT FOCUS(I)/AREA(S) CYST(S) 30 19 22 29 0 22 0 3012010 0220030 0311020 0010000 0021000 1012000 0010000 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 PANCREAS (PA) ........................................ NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 67 59 59 68 13 38 1 LARGE VESSELS MASS(ES) 3000010 0112010 440 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 57 54 55 55 3 33 0 DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) THICKENED WALL EROSION/ULCERATION MASS(ES) 7228261 3102100 4304000 2023010 0010000 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 64 55 57 63 13 35 1 LIGHT FOCUS(I)/AREA(S) EROSION/ULCERATION THICKENED WALL DARK FOCUS(I)/AREA(S) 0001000 3235040 3312030 1100010 DUODENUM (DU) ........................................ NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 ILEUM (IL) ........................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 441 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- CECUM (CE) ........................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 69 60 59 70 12 40 1 DIFFUSELY RED RED FOCUS(I)/AREA(S) THICKENED WALL 0000100 1000000 0010000 COLON (CO) ........................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 RECTUM (RE) .......................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 SKIN (SK) ............................................ NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 67 51 54 66 13 35 1 RED FOCUS(I)/AREA(S) MASS(ES) CRUSTED AREA(S) RAISED AREA(S) ABRASION(S)/ULCERATION(S) 0001000 0422030 0340020 3101000 0110000 442 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 68 59 59 66 13 38 1 THICKENED WALL CALCULUS(I) SEMIFLUID MATERIAL RED FOCUS(I)/AREA(S) DIFFUSELY RED 1102000 2012010 0002000 0001000 0001020 PROSTATE (PR) ........................................ NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 60 55 49 60 13 33 1 LARGE NODULAR DIFFUSELY RED SMALL MOTTLED GELATINOUS MASS(ES) 1002000 9 5 11 5 0 5 0 0001000 0002010 0000010 0001000 0001000 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 58 57 56 62 13 27 1 SMALL GELATINOUS LARGE MOTTLED ** CONTINUED ON NEXT PAGE ** 6234090 1101020 5014030 1010010 443 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 58 57 56 62 13 27 1 DIFFUSELY RED 0001000 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 68 60 60 70 13 39 1 SMALL 2000010 TESTIS (TE) .......................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 64 57 54 61 13 34 1 SMALL CONTAINS FLUID DIFFUSELY RED LARGE RED/YELLOW FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) 6347060 0010000 0001000 1010000 1010000 0001000 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 444 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 OVARY (OV) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 68 59 58 69 13 40 1 LARGE DIFFUSELY RED CYST(S) 1011000 2011000 0100000 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 61 51 57 66 13 36 1 DIFFUSELY RED LARGE MOTTLED 6703020 2200030 1031000 445 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 59 60 70 13 40 1 FRACTURE(S) 0100000 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 69 57 59 69 13 40 1 LARGE MOTTLED DIFFUSELY RED 1200000 1000000 0211000 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 59 70 1 39 1 EDEMA MASS(ES) 0000100 0010010 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 60 55 58 64 13 37 1 MASS(ES) CYST(S) ** CONTINUED ON NEXT PAGE ** 4305010 5210020 446 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 60 55 58 64 13 37 1 THICKENED 1011000 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 65 51 56 66 13 40 1 LARGE MOTTLED DIFFUSELY RED CYSTS 5744000 2100000 0110000 0100000 ANUS (AN) ............................................ NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 39 1 MASS(ES) 0000010 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 66 60 59 69 13 39 1 LARGE MOTTLED CYST(S) DIFFUSELY RED 4000010 1000000 0001000 0010000 447 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- TAIL (TI) ............................................ NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 68 56 59 68 13 37 1 RAISED AREA(S) CRUSTED AREA(S) ABRASION/ULCERATIONS DIFFUSELY DARK LARGE 1100010 1211010 0100000 0000010 0001000 PENIS (PE) ........................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 69 59 60 70 13 40 1 PROTRUDING 1100000 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 FOOT/FOOTPAD(S) (FP) ................................. NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 58 53 48 63 13 30 1 CRUSTED AREA(S) ABRASION(S)/ULCERATION(S) 5654070 7173040 448 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 58 60 70 13 40 1 LARGE MOTTLED 0200000 0100000 LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 68 13 40 1 DIFFUSELY RED LARGE 0002000 0001000 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 59 60 69 13 40 1 THICKENED MASS(ES) 0001000 0100000 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 69 59 59 69 13 40 1 LARGE 1111000 AORTA (AO) ........................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 67 60 60 70 13 40 1 LARGE ** CONTINUED ON NEXT PAGE ** 3000000 449 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** AORTA (AO) ........................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 67 60 60 70 13 40 1 THICKENED WALL 1000000 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 69 58 59 70 13 40 1 LARGE 1210000 SUBCUTANEOUS TIS (SQ) ................................ NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 TONGUE (TO) .......................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 URETER (UE) .......................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 38 1 LARGE 0000020 AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 69 60 60 70 13 40 1 LARGE 1000000 450 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- TESTIS/EPIDIDYM (TP) ................................. NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 PINNA (PN) ........................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 39 1 CRUSTED AREA(S) 0000010 THYROID/PARATHYR (TT) ................................ NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 69 60 60 70 13 40 1 MASS(ES) 1000000 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 59 60 70 13 40 1 LARGE 0100000 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 39 1 MASS(ES) 0000010 451 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 20 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ADRENAL (AD) ......................................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 70 60 60 70 13 40 1 ** END OF LIST ** 452 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 21 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ................................. NUMBER EXAMINED: 70 60 60 70 10 40 0 NO MACROSCOPIC LESIONS DISCHARGE-ORAL STAINS-PERINEUM/PERIANAL BLOOD FILM TAKEN DISCHARGE-OCULAR DISCHARGE-NASAL MALOCCLUSION STAINS - SKIN/HAIRCOAT ANIMAL THIN GI TRACT-DARK FLUID CONTENT BONE MARROW SMEAR TAKEN URINARY BLADDER-SMALL LUMEN TRACHEA-CONTENT FOAMY MATTER GI TRACT-LARGE LUMEN GI TRACT-DARK DRY CONTENT FLUID-THORACIC CAVITY TEETH MISSING/BROKEN/WORN AUTOLYSIS-SEVERE ANIMAL OBESE TAIL - BENT/MISSING FLUID-ABDOMINAL CAVITY 14 9 7 12 6 0 0 0113010 8 7 15 12 0 5 0 49 43 44 53 0 34 0 18 20 20 13 0 11 0 3 5 5 12 0 4 0 2 10 7 12 0 7 0 3 4 2 11 0 5 0 11 10 18 13 0 10 0 0110000 28 18 20 32 5 12 0 0010000 0001000 0000010 0010010 0010020 2112020 0010000 1220020 0000010 0001000 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 453 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 22 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 EYE (EY) ............................................. NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 59 69 10 38 0 OPAQUE CORNEA WHITE INTRAOCULAR MATERIAL RED INTRAOCULAR MATERIAL 0010010 0000010 0001000 BRAIN (BR) ........................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 32 22 23 37 10 16 0 LACERATION COMPRESSED (HYPOTHALAMUS) 0001000 38 38 37 32 0 24 0 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 454 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 23 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LUNG (LU) ............................................ NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 69 58 59 61 10 34 0 DIFFUSELY RED MOTTLED UNCOLLAPSED LIGHT FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) MASS(ES) 1110000 0001000 0112000 0007040 0000010 0000010 KIDNEY (KD) .......................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 61 57 56 63 10 35 0 LARGE PELVIS(ES) ROUGH SURFACE, DIFFUSE MOTTLED DIFFUSELY LIGHT CYST(S) DIFFUSELY DARK CALCULUS(I) GRANULAR MATERIAL MASS(ES) LIGHT FOCUS(I)/AREA(S) 5130020 1000010 1101000 0001010 1004000 0002000 1000000 3110010 0000010 0000010 455 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 24 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LIVER (LI) ........................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 55 44 52 41 7 23 0 LIGHT FOCUS(I)/AREA(S) LARGE MOTTLED DIFFUSELY DARK DEPRESSED FOCUS(I)/AREA(S) DIFFUSELY LIGHT MASS(ES) RED FOCUS(I)/AREA(S) DARK FOCUS(I)/AREA(S) RAISED FOCUS(I)/AREA(S) CYST(S) ADHESION(S) MASS #2 0326060 1103200 3218140 0007210 0100000 4201020 0103000 7868070 0103010 0010000 0001000 1000000 0001010 HEART (HT) ........................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 69 10 40 0 DIFFUSELY LIGHT 0001000 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 456 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 25 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 70 40 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 66 59 54 67 10 39 0 LARGE MOTTLED DIFFUSELY RED DIFFUSELY DARK RED FOCUS(I)/AREA(S) GELATINOUS 1120000 1011000 1022000 1010000 0010000 0000010 SPLEEN (SP) .......................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 69 60 59 67 10 38 0 SMALL LARGE LIGHT FOCUS(I)/AREA(S) 1000010 0012010 0001000 THYMUS (TH) .......................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 69 59 60 69 9 40 0 RED FOCUS(I)/AREA(S) MOTTLED MISSING/NOT IDENTIFIED ** CONTINUED ON NEXT PAGE ** 0000100 1000000 0100000 457 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 26 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** THYMUS (TH) .......................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 69 59 60 69 9 40 0 LARGE CYST(S) 1000000 0001000 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 THYROID (TY) ......................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 68 58 58 69 10 40 0 DIFFUSELY RED MASS(ES) LARGE DIFFUSELY DARK 0100000 1000000 1121000 1000000 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 69 10 40 0 LARGE 0001000 TRACHEA (TR) ......................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 458 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 57 46 48 65 10 34 0 DIFFUSELY RED MASS(ES) DARK FOCUS(I)/AREA(S) LARGE CYST(S) LIGHT FOCUS(I)/AREA(S) DIFFUSELY DARK SMALL RED FOCUS(I)/AREA(S) DIFFUSELY LIGHT 1210000 1201010 0010010 8882030 0110000 0112000 3001000 0110010 2100010 0010000 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 PITUITARY (PI) ....................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 28 16 12 27 10 7 0 MISSING/NOT IDENTIFIED MASS(ES) DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) LARGE RAISED AREA(S) 0001000 38 36 36 32 0 24 0 3242020 0343040 2586050 0100000 459 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 28 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 PANCREAS (PA) ........................................ NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 58 60 70 10 40 0 LARGE VESSELS MASS(ES) 0100000 0100000 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 63 55 55 54 9 34 0 DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) THICKENED WALL EROSION/ULCERATION LIGHT FOCUS(I)/AREA(S) 3 2 3 11 1 1 0 2212010 0200010 1012020 1001010 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 63 55 59 66 10 35 0 LIGHT FOCUS(I)/AREA(S) ** CONTINUED ON NEXT PAGE ** 3000000 460 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 29 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 63 55 59 66 10 35 0 EROSION/ULCERATION THICKENED WALL DARK FOCUS(I)/AREA(S) RAISED AREA(S) 2313040 1201020 1101010 1000000 DUODENUM (DU) ........................................ NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 69 57 60 70 10 39 0 MASS(ES) DIVERTICULUM(A) LARGE LYMPHOID AREA(S) 0000010 1200000 0100000 ILEUM (IL) ........................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 59 60 70 10 40 0 LARGE LYMPHOID AREA(S) 0100000 461 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 30 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- CECUM (CE) ........................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 69 60 60 70 10 40 0 SMALL 1000000 COLON (CO) ........................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 RECTUM (RE) .......................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 SKIN (SK) ............................................ NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 69 57 58 68 10 38 0 ALOPECIA-FOCAL MASS(ES) CRUSTED AREA(S) ALOPECIA-DIFFUSE 0011000 0211010 1100010 0000010 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 PROSTATE (PR) ........................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 462 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 31 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 TESTIS (TE) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 35 25 28 46 10 19 0 MASS(ES) CYST(S) MASS #2 THICKENED MASS #3 MASS #4 MASS #5 MASS #6 28 27 26 20 0 17 0 12 8 5 3 0 4 0 6 10 15 9 0 4 0 15 15 15 8 0 6 0 3445000 0243000 0021000 0001000 463 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 32 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- CERVIX (CV) .......................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 66 56 57 66 10 37 0 MASS(ES) LARGE FIRM LIGHT FOCUS(I)/AREA(S) 0221030 4212000 0010000 0001000 UTERUS (UT) .......................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 53 50 50 52 9 33 0 LUMEN FILLED WITH FLUID CYST(S) THICKENED WALL LARGE, DIFFUSE MASS(ES) SEMIFLUID MATERIAL LARGE, FOCAL RED FOCUS(I)/AREA(S) SEMISOLID MATERIAL 3028110 10 9 6 5 0 5 0 1014000 3002010 3212010 0001000 0001000 0010000 0010000 OVARY (OV) ........................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 63 49 49 49 10 30 0 CYST(S) MISSING/NOT IDENTIFIED MASS(ES) ** CONTINUED ON NEXT PAGE ** 7 10 11 19 0 10 0 0101000 0001000 464 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 33 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** OVARY (OV) ........................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 63 49 49 49 10 30 0 MASS #2 0001000 VAGINA (VA) .......................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 67 55 55 67 10 33 0 MASS(ES) SEMIFLUID MATERIAL 0020020 3533050 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 59 60 69 10 39 0 LARGE DIFFUSELY RED 0101000 0100010 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 62 54 51 63 10 33 0 DIFFUSELY RED RED FOCUS(I)/AREA(S) LARGE MOTTLED MISSING/NOT IDENTIFIED 6253040 0100000 1220020 1123020 0001000 465 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 34 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 59 60 70 10 40 0 DIFFUSELY RED 0100000 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 57 59 68 10 38 0 MASS(ES) 0312020 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 59 60 69 10 40 0 FRACTURE(S) DEPRESSED AREA(S) 0001000 0100000 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 466 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 35 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 68 59 59 70 10 37 0 LARGE MOTTLED 1010030 2100000 ANUS (AN) ............................................ NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 69 59 60 69 10 38 0 LARGE DIFFUSELY RED SMALL 1100010 1101010 1000000 TAIL (TI) ............................................ NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 59 67 10 39 0 RAISED AREA(S) CRUSTED AREA(S) 0001000 0012010 PENIS (PE) ........................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 467 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 36 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 69 59 60 68 10 39 0 LARGE DIFFUSELY RED DIFFUSELY DARK 1101010 1100000 0002000 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 59 70 10 40 0 MASS(ES) 0010000 FOOT/FOOTPAD(S) (FP) ................................. NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 63 57 57 69 10 36 0 CRUSTED AREA(S) ABRASION(S)/ULCERATION(S) 6031040 1300000 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 59 53 55 64 10 38 0 LARGE MOTTLED CYST(S) DIFFUSELY RED 5133010 5523010 1000000 1202000 468 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 37 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 69 60 60 70 10 40 0 MASS(ES) 1000000 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 67 60 59 70 10 40 0 LARGE 3010000 AORTA (AO) ........................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 39 0 FIRM 0000010 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 59 70 10 40 0 LARGE 0010000 469 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 38 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- SUBCUTANEOUS TIS (SQ) ................................ NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 59 70 10 40 0 MASS(ES) 0010000 TONGUE (TO) .......................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 39 0 MASS(ES) 0000010 URETER (UE) .......................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 TESTIS/EPIDIDYM (TP) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 PINNA (PN) ........................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 69 10 40 0 RED FOCUS(I)/AREA(S) 0001000 470 Table 39 Incidence of Macroscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 39 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- THYROID/PARATHYR (TT) ................................ NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 69 10 40 0 MISSING/NOT IDENTIFIED 0001000 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 ADRENAL (AD) ......................................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 70 60 60 70 10 40 0 ** END OF LIST ** 471 Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Author: Peter J. Thomford, PhD Study Completion Date: December 13, 2001 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-212 Sponsor Study Identification: 3M T-6316.1 Volume II of XII Page 472 of 4873 Covance 6329-212 3M T-6316.1 VOLUME II OF XII CONTENTS Page TABLES 40 Incidence of Microscopic Observations - Week 8 Sacrifice................................ 474 41 Incidence of Microscopic Observations - Week 14 Sacrifice.............................. 486 42 Incidence of Microscopic Observations - Week 53 Sacrifice.............................. 499 43 Incidence of Microscopic Observations - Terminal and Recovery Sacrifices...... 514 44 Incidence of Microscopic Observations - Unscheduled Deaths........................... 537 45 Incidence of Microscopic Observations - All Animals ....................................... 583 46 Incidence of Severity of Selected Microscopic Observations - Week 8 Sacrifice.......................................................................................................... 635 47 Incidence of Severity of Selected Microscopic Observations - Week 14 Sacrifice.......................................................................................................... 641 48 Incidence of Severity of Selected Microscopic Observations - Week 53 Sacrifice.......................................................................................................... 647 49 Incidence of Severity of Selected Microscopic Observations - Terminal and Recovery Sacrifice.......................................................................................... 653 50 Incidence of Severity of Selected Microscopic Observations - Unscheduled Deaths ............................................................................................................ 661 51 Incidence of Severity of Selected Microscopic Observations - All Animals........ 668 APPENDIX 1.............................................................................................................. 677 Protocol Deviations ................................................................................................. 678 473 TABLE 40 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- ** TOP OF LIST ** EYE (EY) ............................................. NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 BRAIN (BR) ........................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 LIVER (LI) ........................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 3 0 4 0 --HEMORRHAGE --HYPERTROPHY, CENTRILOBULAR, --INFLAMMATION, CHRONIC --NECROSIS, HEPATOCELLULAR --VACUOLATION, HEPATOCELLULAR HEPATOCELLULAR 0301 0515 2000 0301 0100 LIVER, OIL RED O (LI1) ............................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 KIDNEY (KD) .......................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 3 4 5 4 --CAST, PROTEINACEOUS --CYST --MINERALIZATION, TUBULAR --NEPHROPATHY, CHRONIC PROGRESSIVE 1000 1100 0001 0100 474 TABLE 40 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- SPLEEN (SP) .......................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 PANCREAS (PA) ........................................ NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 4 5 5 5 --HEMORRHAGE 1000 OVARY (OV) ........................................... NUMBER EXAMINED: 0 0 5 5 NOT REMARKABLE: 0 0 5 5 TESTIS (TE) .......................................... NUMBER EXAMINED: 5 5 0 0 NOT REMARKABLE: 5 5 0 0 475 TABLE 40 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 THYMUS (TH) .......................................... NUMBER EXAMINED: 1 0 0 0 NOT REMARKABLE: 1 0 0 0 LUNG (LU) ............................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 476 TABLE 40 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- HEART (HT) ........................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 TRACHEA (TR) ......................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 THYROID (TY) ......................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 477 TABLE 40 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- PITUITARY (PI) ....................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 1 0 0 0 NOT REMARKABLE: 1 0 0 0 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 1 0 0 0 NOT REMARKABLE: 1 0 0 0 DUODENUM (DU) ........................................ NUMBER EXAMINED: 4 1 0 0 NOT REMARKABLE: 4 1 0 0 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 1 0 0 0 NOT REMARKABLE: 1 0 0 0 478 TABLE 40 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- ILEUM (IL) ........................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 CECUM (CE) ........................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 COLON (CO) ........................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 RECTUM (RE) .......................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 SKIN (SK) ............................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 479 TABLE 40 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- CERVIX (CV) .......................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 PROSTATE (PR) ........................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 1 0 0 0 NOT REMARKABLE: 1 0 0 0 CAUSE OF DEATH (CD) .................................. NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 0 0 0 0 --SCHEDULED SACRIFICE 5555 480 TABLE 40 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 0 0 0 1 NOT REMARKABLE: 0 0 0 0 --HEMORRHAGE 0001 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 HEMATO NEOPLASIA (HN) ................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 MESENTERY (MY) ....................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 481 TABLE 40 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 TAIL (TI) ............................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 ANUS (AN) ............................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 482 TABLE 40 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- FOOT/FOOTPAD(S) (FP) ................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 AORTA (AO) ........................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 PENIS (PE) ........................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 PREPUTIAL GLAND (PG) ................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 483 TABLE 40 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 URETER (UE) .......................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 PINNA (PN) ........................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 484 TABLE 40 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- SUBCUTANEOUS TIS (SQ) ................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 TONGUE (TO) .......................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 ** END OF LIST ** 485 TABLE 41 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** EYE (EY) ............................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 BRAIN (BR) ........................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 LIVER (LI) ........................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 3 1 1 0 3 1 3 2 --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR --INFLAMMATION, CHRONIC --VACUOLATION, HEPATOCELLULAR 10000000 10001000 01350002 11301422 02210000 LIVER, OIL RED O (LI1) ............................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 KIDNEY (KD) .......................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 3 3 5 5 5 5 4 4 --CAST, PROTEINACEOUS --CYST --DEGENERATION, TUBULAR --DILATATION, PELVIC 10000000 00000001 01000000 10000000 486 TABLE 41 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** KIDNEY (KD) .......................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 3 3 5 5 5 5 4 4 --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, CHRONIC --MINERALIZATION, TUBULAR --PYELONEPHRITIS 10000000 01000000 00000010 10000000 SPLEEN (SP) .......................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 PANCREAS (PA) ........................................ NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 4 5 4 5 5 5 5 5 --FIBROSIS --PIGMENT 10000000 10100000 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 3 2 4 4 5 5 5 4 --VACUOLATION 23110001 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 487 TABLE 41 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 4 5 5 5 4 5 --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 00100010 00000010 OVARY (OV) ........................................... NUMBER EXAMINED: 0 0 0 0 5 5 5 5 NOT REMARKABLE: 0 0 0 0 5 5 5 4 --HEMORRHAGE 00000001 TESTIS (TE) .......................................... NUMBER EXAMINED: 5 5 5 5 0 0 0 0 NOT REMARKABLE: 5 5 4 5 0 0 0 0 --ATROPHY/DEGENERATION 00100000 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 488 TABLE 41 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 THYMUS (TH) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 LUNG (LU) ............................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 HEART (HT) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 489 TABLE 41 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- TRACHEA (TR) ......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 THYROID (TY) ......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 1 0 0 0 0 0 0 0 NOT REMARKABLE: 1 0 0 0 0 0 0 0 PITUITARY (PI) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 490 TABLE 41 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 1 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 --EDEMA 10000000 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 1 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 --EDEMA --EROSION --HEMORRHAGE 10000000 10000000 10000000 DUODENUM (DU) ........................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 ILEUM (IL) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 CECUM (CE) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 491 TABLE 41 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- COLON (CO) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 RECTUM (RE) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 SKIN (SK) ............................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 492 TABLE 41 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 PROSTATE (PR) ........................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 CAUSE OF DEATH (CD) .................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 0 0 0 0 0 0 0 0 --SCHEDULED SACRIFICE 55555555 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 493 TABLE 41 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 HEMATO NEOPLASIA (HN) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 MESENTERY (MY) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 494 TABLE 41 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 TAIL (TI) ............................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 ANUS (AN) ............................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 FOOT/FOOTPAD(S) (FP) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 495 TABLE 41 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 AORTA (AO) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 PENIS (PE) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 PREPUTIAL GLAND (PG) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 496 TABLE 41 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 URETER (UE) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 PINNA (PN) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 SUBCUTANEOUS TIS (SQ) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 TONGUE (TO) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 497 TABLE 41 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- LN, OTHER (LN) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 ** END OF LIST ** 498 TABLE 42 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ** TOP OF LIST ** EYE (EY) ............................................. NUMBER EXAMINED: 5 10 5 10 NOT REMARKABLE: 5 10 5 10 BRAIN (BR) ........................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 --COMPRESSION, VENTRAL 00 LIVER (LI) ........................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 2 0 --DEGENERATION, CYSTIC --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC --HYPERPLASIA, BILE DUCT --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR --INFLAMMATION, CHRONIC --NECROSIS, HEPATOCELLULAR --STASIS, BILE --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR 00 00 00 0 10 31 01 01 09 00 LIVER, OIL RED O (LI1) ............................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 5 10 58 02 5 10 42 01 01 01 07 11 02 02 02 01 00 00 499 TABLE 42 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- KIDNEY (KD) .......................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 0 7 5 10 15 --CAST, PROTEINACEOUS --INFLAMMATION, CHRONIC --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --NEPHROPATHY, CHRONIC PROGRESSIVE 3103 0020 1122 0021 2100 SPLEEN (SP) .......................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 PANCREAS (PA) ........................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 4 8 --ATROPHY, ACINAR --FIBROSIS --INFLAMMATION, CHRONIC --PIGMENT 10 01 01 01 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 3 9 --ANGIECTASIS --DEGENERATION, CYSTIC, CORTICAL --FIBROSIS --HEMORRHAGE 00 10 00 10 5 10 5 10 00 00 00 00 5 10 08 20 02 01 40 500 TABLE 42 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 5 10 5 10 NOT REMARKABLE: 3 9 0 8 --HYPERTROPHY, CORTICAL CELL --ONE EXAMINED --VACUOLATION 1000 0010 1100 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 9 5 10 4 10 --HEMORRHAGE --ONE EXAMINED --B-PHEOCHROMOCYTOMA 0100 0010 0100 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 9 5 10 59 --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 0101 0100 OVARY (OV) ........................................... NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 5 7 --CYST --HEMORRHAGE 0002 0002 501 TABLE 42 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- TESTIS (TE) .......................................... NUMBER EXAMINED: 5 10 0 0 NOT REMARKABLE: 5 9 0 0 --ATROPHY/DEGENERATION 0100 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 502 TABLE 42 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- THYMUS (TH) .......................................... NUMBER EXAMINED: 5 9 5 10 NOT REMARKABLE: 4 9 5 9 --FIBROSIS --HEMORRHAGE 0001 1000 LUNG (LU) ............................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 4 7 5 10 48 --HYPERPLASIA, MEDIAL, ARTERIAL/ARTERIOLAR --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, MACROPHAGE, PIGMENTED --MINERALIZATION, VASCULAR 0100 1112 0001 1100 HEART (HT) ........................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 9 5 10 5 10 --DEGENERATION/NECROSIS 0100 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 503 TABLE 42 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- TRACHEA (TR) ......................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 THYROID (TY) ......................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 3 7 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL 01 22 00 00 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 4 10 --HYPERPLASIA 10 PITUITARY (PI) ....................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 3 4 --CYST --HEMORRHAGE --HYPERPLASIA --B-ADENOMA 00 02 03 23 5 10 33 00 26 01 01 59 59 00 5 10 35 01 01 00 24 504 TABLE 42 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 59 --INFLAMMATION, CHRONIC 0001 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 9 --EDEMA --EROSION 00 01 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 48 10 02 5 10 5 10 DUODENUM (DU) ........................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 505 TABLE 42 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ILEUM (IL) ........................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 CECUM (CE) ........................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 COLON (CO) ........................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 9 --PARASITISM 01 RECTUM (RE) .......................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 9 --PARASITISM 01 SKIN (SK) ............................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 00 5 10 4 10 10 5 10 5 10 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 3 8 --GALACTOCELE --B-FIBROADENOMA --M-CARCINOMA 0021 0010 0001 506 TABLE 42 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 5 10 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 3 8 --DILATATION --HYPERPLASIA, CYSTIC, ENDOMETRIAL 0021 0001 VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 5 10 PROSTATE (PR) ........................................ NUMBER EXAMINED: 5 10 0 0 NOT REMARKABLE: 3 10 0 0 --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE 1000 1000 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 5 10 0 0 NOT REMARKABLE: 5 10 0 0 507 TABLE 42 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 5 10 0 0 NOT REMARKABLE: 5 10 0 0 CAUSE OF DEATH (CD) .................................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 0 0 --SCHEDULED SACRIFICE 5 10 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 1 1 NOT REMARKABLE: 0 0 --HEMORRHAGE 11 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 00 00 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 --FIBROSIS --NECROSIS, FAT 00 00 5 10 00 5 10 10 00 10 02 00 02 02 01 00 01 01 508 TABLE 42 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 HEMATO NEOPLASIA (HN) ................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 MESENTERY (MY) ....................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 0 0 0 1 NOT REMARKABLE: 0 0 0 1 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 509 TABLE 42 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 TAIL (TI) ............................................ NUMBER EXAMINED: 0 0 0 1 NOT REMARKABLE: 0 0 0 0 --CYST, EPIDERMAL INCLUSION --INFLAMMATION, ACUTE 0001 0001 ANUS (AN) ............................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 FOOT/FOOTPAD(S) (FP) ................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 510 TABLE 42 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 AORTA (AO) ........................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 PENIS (PE) ........................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 PREPUTIAL GLAND (PG) ................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 511 TABLE 42 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 URETER (UE) .......................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 PINNA (PN) ........................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 SUBCUTANEOUS TIS (SQ) ................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 TONGUE (TO) .......................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 512 TABLE 42 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- LN, OTHER (LN) ....................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 ** END OF LIST ** 513 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** EYE (EY) ............................................. NUMBER EXAMINED: 14 1 0 17 0 13 0 0 20 0 NOT REMARKABLE: 12 0 0 16 0 11 0 0 17 0 --CATARACT --HEMORRHAGE --HYPERPLASIA, CORNEAL EPITHELIUM --INFLAMMATION, ACUTE --INFLAMMATION, ACUTE, CILIARY --INFLAMMATION, ACUTE, CORNEAL --MINERALIZATION, CORNEAL --ULCERATION, CORNEAL --VASCULARIZATION, CORNEAL 1000000000 0000000010 1000000010 0000010000 0100000010 1101000000 1000000000 0100020000 0100000010 BRAIN (BR) ........................................... NUMBER EXAMINED: 14 4 3 17 4 13 6 8 20 6 NOT REMARKABLE: 12 0 1 13 0 8 1 1 11 1 --COMPRESSION, VENTRAL --DILATATION, VENTRICULAR --B-GRANULAR CELL TUMOR 2423455795 0101033561 0001000000 LIVER (LI) ........................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 NOT REMARKABLE: 4 3 1 1 2 6 2 7 3 5 --CYST --CYST, BILIARY --DEGENERATION, CYSTIC --FIBROSIS --FIBROSIS, CAPSULAR 1000000010 0000001000 3 5 12 12 1 0 2 0 1 1 0010100001 0000010000 514 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 NOT REMARKABLE: 4 3 1 1 2 6 2 7 3 5 --FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --HEMORRHAGE --HYPERPLASIA, BILE DUCT --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) --NECROSIS, HEPATOCELLULAR --PIGMENT --TELANGIECTASIS --THROMBUS --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR --M-CARCINOMA, HEPATOCELLULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1110000100 3 10 11 5 1 0 4 2 5 1 0021002110 0211211101 4764111371 0005000050 0000100000 4 13 12 8 3 1 2 2 8 1 0201001002 0120110101 0111100010 2620012044 0100000010 4 8 9 13 1 4 6 2 13 2 1213001142 0000000010 1000000000 LIVER, OIL RED O (LI1) ............................... NUMBER EXAMINED: 5 0 0 5 0 5 0 0 5 0 NOT REMARKABLE: 1 0 0 1 0 0 0 0 0 0 --POSITIVE OIL RED O STAINING 4004050050 515 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- KIDNEY (KD) .......................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 NOT REMARKABLE: 0 0 1 6 0 0 1 3 4 1 --BACTERIAL COLONIES --CAST, PROTEINACEOUS --CYST --DILATATION, PELVIC --INFARCT --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --MINERALIZATION --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --NEPHROPATHY, CHRONIC PROGRESSIVE --PYELONEPHRITIS --THROMBUS --M-LIPOSARCOMA 0000000001 2752358364 1430000020 0141210001 0210100000 1100000000 0001000000 1000000000 1 2 4 1 0 12 10 10 13 8 0000000001 12 14 15 8 7 2 0 1 4 3 0100010011 1000000000 0000100000 SPLEEN (SP) .......................................... NUMBER EXAMINED: 14 1 0 17 0 13 0 0 20 0 NOT REMARKABLE: 13 1 0 17 0 12 0 0 20 0 --PIGMENT, INCREASED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0000010000 1000000000 PANCREAS (PA) ........................................ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 NOT REMARKABLE: 5 8 14 8 3 10 8 12 18 9 --ATROPHY, ACINAR 6767513223 516 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** PANCREAS (PA) ........................................ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 NOT REMARKABLE: 5 8 14 8 3 10 8 12 18 9 --CYST --HEMORRHAGE --HYPERPLASIA, ACINAR --HYPERTROPHY, VASCULAR --INFILTRATE, EOSINOPHILS --INFLAMMATION, CHRONIC --INFLAMMATION, VASCULAR --PIGMENT --B-ADENOMA, ISLET CELL --M-CARCINOMA, ISLET CELL 0100000000 2100011000 1200010000 2000100000 1000000000 2000000000 2200100000 0222200000 1523114110 0010000000 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 14 2 3 17 0 13 4 4 20 2 NOT REMARKABLE: 2 0 2 10 0 0 0 0 3 0 --ADRENAL TISSUE, ECTOPIC --ANGIECTASIS --ATROPHY, CORTICAL --CYST --DEGENERATION, CYSTIC, CORTICAL --HEMORRHAGE --HYPERPLASIA, CORTICAL --HYPERTROPHY, CORTICAL CELL --MINERALIZATION --NECROSIS, CORTICAL --PIGMENT 0001000000 4101072191 0000000011 0000001000 3111062371 6 2 0 1 0 13 4 4 16 2 3001000020 2000000000 1000010011 0000000001 0100041011 517 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 14 2 3 17 0 13 4 4 20 2 NOT REMARKABLE: 2 0 2 10 0 0 0 0 3 0 --THROMBUS --VACUOLATION --B-ADENOMA --M-CARCINOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 2100043031 10 1 0 6 0 1 0 1 1 0 0000000100 0000000001 1000000000 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 14 1 3 17 1 13 3 4 20 2 NOT REMARKABLE: 14 0 0 16 0 13 2 4 18 1 --NECROSIS --ONE EXAMINED --B-PHEOCHROMOCYTOMA --M-MALIGNANT PHEOCHROMOCYTOMA 0010000000 0000000001 0121000020 0010101000 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 14 1 2 17 0 13 0 3 20 0 NOT REMARKABLE: 10 0 1 15 0 9 0 2 19 0 --DILATATION, SINUSOIDAL --EDEMA --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --NECROSIS --THROMBUS --M-HEMANGIOSARCOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1001000100 0000010000 0111010110 0110020010 1000000000 1000000000 1100000000 1001000000 518 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- OVARY (OV) ........................................... NUMBER EXAMINED: 0 0 0 0 0 13 5 4 20 4 NOT REMARKABLE: 0 0 0 0 0 11 0 2 9 1 --CYST --HEMORRHAGE --MINERALIZATION --ONE EXAMINED --PIGMENTED --THROMBUS --B-GRANULOSA/THECA CELL TUMOR 0000025283 0000001010 0000000020 0000000001 0000000020 0000000020 0000000010 TESTIS (TE) .......................................... NUMBER EXAMINED: 14 22 22 17 10 0 0 0 0 0 NOT REMARKABLE: 12 16 18 10 9 0 0 0 0 0 --ATROPHY/DEGENERATION --HEMORRHAGE --INFLAMMATION, CHRONIC --MINERALIZATION --MINERALIZATION, VASCULAR --B-INTERSTITIAL CELL TUMOR 1333000000 0011000000 1310000000 0112000000 0002100000 1131000000 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 519 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 12 0 0 20 0 --HEMORRHAGE 0000010000 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 12 0 0 17 0 --HYPERPLASIA, CARTILAGE 0000010030 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 THYMUS (TH) .......................................... NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 12 0 0 17 0 11 0 0 7 0 --CYST --M-THYMIC CARCINOMA 2 0 0 0 0 2 0 0 12 0 0000000010 LUNG (LU) ............................................ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 NOT REMARKABLE: 10 14 11 11 4 9 6 10 12 5 --FIBROSIS, PLEURAL/SUBPLEURAL 1020210000 520 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LUNG (LU) ............................................ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 NOT REMARKABLE: 10 14 11 11 4 9 6 10 12 5 --HEMORRHAGE --HYPERPLASIA, TYPE II PNEUMOCYTE --INFLAMMATION, CHRONIC --INFILTRATE, MACROPHAGE, ALVEOLAR --MINERALIZATION --MINERALIZATION, VASCULAR --NECROSIS --N-ADRENAL CORTICAL CARCINOMA --N-MALIGNANT PHEOCHROMOCYTOMA 1000000001 0002002001 2020020003 3111322153 0101100001 0773404442 0000000001 0000000001 0000001000 HEART (HT) ........................................... NUMBER EXAMINED: 14 1 0 17 0 13 0 0 20 0 NOT REMARKABLE: 9 0 0 15 0 11 0 0 20 0 --DEGENERATION/NECROSIS --FIBROSIS 4102020000 4100020000 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 14 1 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 --DEGENERATION/NECROSIS 0100000000 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 521 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- TRACHEA (TR) ......................................... NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 THYROID (TY) ......................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 NOT REMARKABLE: 6 8 9 4 2 7 6 8 8 3 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --HEMORRHAGE --HYPERPLASIA, C-CELL --HYPERPLASIA, FOLLICULAR CELL --INFLAMMATION, GRANULOMATOUS --MINERALIZATION --NECROSIS --ONE EXAMINED --PIGMENT --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL --M-CARCINOMA, C-CELL --M-CARCINOMA, FOLLICULAR CELL 1134211021 2854232157 0101023011 3120200021 0002000001 0100000000 0001000000 0010000000 0000000010 1000000000 3666334445 0213100210 0010000000 0010000000 522 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- PARATHYROID (PT) ..................................... NUMBER EXAMINED: 14 2 0 15 0 13 0 0 20 0 NOT REMARKABLE: 12 1 0 15 0 12 0 0 20 0 --HYPERPLASIA --B-ADENOMA 2100000000 0000010000 PITUITARY (PI) ....................................... NUMBER EXAMINED: 14 NOT REMARKABLE: 1 5 10 17 002 6 13 11 14 20 10 010050 --CYST --HEMORRHAGE --HYPERPLASIA --PIGMENT --B-ADENOMA 1021000131 6 3 5 7 6 8 8 8 12 7 1000000000 0000022121 12 5 10 15 6 12 11 14 15 10 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 13 0 0 13 0 11 0 0 18 0 --HEMORRHAGE --INFLAMMATION, CHRONIC --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0000010000 0004020020 1000000000 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 523 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 14 3 1 17 1 13 0 0 20 5 NOT REMARKABLE: 12 1 0 15 0 12 0 0 19 5 --EDEMA --EROSION --FIBROSIS --MINERALIZATION --INFLAMMATION, CHRONIC-ACTIVE 1212110000 0011100010 0010000000 1100000000 0010000000 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 14 1 1 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 12 0 0 20 0 --EDEMA --EROSION --INFLAMMATION, ACUTE --ULCERATION 0110010000 0010010000 0100000000 0100000000 DUODENUM (DU) ........................................ NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 14 0 0 17 0 13 1 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 --HYPERPLASIA, GALT (GUT ASSOCIATED LYMPHOID TISSUE) 0000001000 524 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ILEUM (IL) ........................................... NUMBER EXAMINED: 14 0 0 17 0 13 1 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 --HYPERPLASIA, GALT (GUT ASSOCIATED LYMPHOID TISSUE) 0000001000 CECUM (CE) ........................................... NUMBER EXAMINED: 14 0 1 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 1 17 0 12 0 0 20 0 --HEMORRHAGE 0000010000 COLON (CO) ........................................... NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 RECTUM (RE) .......................................... NUMBER EXAMINED: 14 0 0 16 0 13 0 0 20 0 NOT REMARKABLE: 11 0 0 12 0 13 0 0 18 0 --PARASITISM 3004000020 SKIN (SK) ............................................ NUMBER EXAMINED: 14 7 5 17 2 12 2 1 20 0 NOT REMARKABLE: 12 1 0 13 0 10 1 1 17 0 --ACUTE INFLAMMATORY EXUDATE --CYST, EPIDERMAL INCLUSION --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC 0000100000 1010000000 0000000010 1011100010 0220011020 0000100000 525 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SKIN (SK) ............................................ NUMBER EXAMINED: 14 7 5 17 2 12 2 1 20 0 NOT REMARKABLE: 12 1 0 13 0 10 1 1 17 0 --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --B-FIBROMA --B-KERATOACANTHOMA --B-LIPOMA --B-PAPILLOMA, SQUAMOUS CELL --M-CARCINOMA, SQUAMOUS CELL --M-SEBACEOUS GLAND CARCINOMA 0000010010 0220211030 1110000000 0141000000 0102000000 0201000000 0100000000 0000001000 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 0 0 0 0 12 13 15 20 12 NOT REMARKABLE: 0 0 0 0 0 1 2 3 7 1 --GALACTOCELE --HEMORRHAGE --HYPERPLASIA, GLANDULAR --MINERALIZATION --NECROSIS --PIGMENT --B-ADENOMA --B-FIBROADENOMA --M-CARCINOMA 0 0 0 0 0 8 7 10 11 11 0000011211 0000000220 0000011011 0000010100 0000001101 0000041410 0000088574 0000012532 526 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 14 22 22 17 10 13 13 14 20 12 NOT REMARKABLE: 13 10 12 16 7 11 13 14 20 10 --CALCULUS --DESQUAMATION, TRANSITIONAL EPITHELIUM --EDEMA --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --B-PAPILLOMA, TRANSITIONAL CELL --B-LEIOMYOMA --M-CARCINOMA, TRANSITIONAL CELL 0 10 8 0 3 0 0 0 0 0 0010000000 0010000000 0110000001 0111000002 0110000000 1000000000 0000010000 0000010000 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 0 0 0 0 13 2 1 20 2 NOT REMARKABLE: 0 0 0 0 0 11 0 0 18 0 --CYST --DILATATION --HYPERTROPHY, SMOOTH MUSCLE --PIGMENT --B-LEIOMYOMA --B-POLYP, ENDOMETRIAL STROMAL --M-LEIOMYOSARCOMA 0000010000 0000000010 0000001100 0000010000 0000000011 0000001010 0000000001 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 0 0 0 13 4 5 20 4 NOT REMARKABLE: 0 0 0 0 0 4 0 0 9 0 --CYST, KERATIN 0000000010 527 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 0 0 0 13 4 5 20 4 NOT REMARKABLE: 0 0 0 0 0 4 0 0 9 0 --DILATATION --HEMORRHAGE --HYPERPLASIA, CYSTIC, ENDOMETRIAL --HYPERPLASIA, ENDOMETRIAL --INFLAMMATION, CHRONIC --PIGMENT --B-LEIOMYOMA --B-POLYP, ENDOMETRIAL, STROMAL --M-CARCINOMA --M-LEIOMYOSARCOMA 0000073462 0000000100 0000031043 0000010010 0000020000 0000010000 0000010000 0000012120 0000000100 0000000010 VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 0 0 0 13 0 1 19 1 NOT REMARKABLE: 0 0 0 0 0 12 0 1 19 0 --B-LEIOMYOMA --I-LEIOMYOSARCOMA 0000010000 0000000001 PROSTATE (PR) ........................................ NUMBER EXAMINED: 14 3 5 17 1 0 0 0 0 0 NOT REMARKABLE: 2 0 0 6 0 0 0 0 0 0 --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --MINERALIZATION, LUMINAL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 2122000000 2011100000 7128000000 0102000000 1000000000 528 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 14 0 0 17 0 0 0 0 0 0 NOT REMARKABLE: 12 0 0 16 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --SECRETION, INCREASED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001000000 1000000000 1000000000 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 14 0 0 17 0 0 0 0 0 0 NOT REMARKABLE: 12 0 0 15 0 0 0 0 0 0 --HYPOSPERMIA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1002000000 1000000000 CAUSE OF DEATH (CD) .................................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --SCHEDULED SACRIFICE --PITUITARY ADENOMA 14 22 22 17 10 13 13 15 19 12 0000000010 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 3 7 3 2 2 4 3 4 2 5 NOT REMARKABLE: 0 2 0 0 0 2 0 0 0 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0101200102 2531122425 0001101000 0000001000 1000000000 529 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 2 1 1 2 0 1 0 0 2 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 1112000000 1000010010 0000000010 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 1 0 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --NECROSIS, FAT 0000000101 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 HEMATO NEOPLASIA (HN) ................................ NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 13 0 0 16 0 13 0 0 20 0 --M-LYMPHOMA 1001000000 MESENTERY (MY) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 530 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 0 1 1 0 0 0 1 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --N-ISLET CELL CARCINOMA 0010000000 0110001000 0000000010 0010000000 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 2 4 3 0 0 3 4 3 2 2 NOT REMARKABLE: 0 0 0 0 0 2 4 2 2 1 --DILATATION, SINUSOIDAL --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC 2420010000 0010000101 0010010000 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 0 0 1 0 0 1 1 0 1 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --HEMORRHAGE 0010011011 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 3 1 0 3 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --GALACTOCELE --MINERALIZATION --NECROSIS --PIGMENT --B-ADENOMA 0101100000 0000100000 1000000000 1001000000 0001000000 531 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 3 1 0 3 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --B-FIBROADENOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 2000000000 1000000000 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 0 3 0 3 0 3 4 6 6 3 NOT REMARKABLE: 0 2 0 2 0 1 3 3 2 3 --DILATATION, SINUSOIDAL --EDEMA --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED 0000001100 0000000100 0001010220 0100010010 0000000020 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 TAIL (TI) ............................................ NUMBER EXAMINED: 0 1 0 1 0 0 0 0 0 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --EROSION --EXOSTOSES --INFLAMMATION, ACUTE 0100000001 0001000000 0100000001 532 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 20 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ANUS (AN) ............................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 FOOT/FOOTPAD(S) (FP) ................................. NUMBER EXAMINED: 2 5 6 2 4 3 0 0 1 2 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --EDEMA --EROSION --EXOSTOSES --FIBROSIS --INFLAMMATION, --INFLAMMATION, --PIGMENT ACUTE CHRONIC ACTIVE 1260010000 2562330012 1421210000 1341320002 2561430002 0001000010 0000100000 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 0 0 1 0 0 1 1 1 1 0 NOT REMARKABLE: 0 0 1 0 0 0 1 1 0 0 --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 0000000010 0000010010 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 0 1 1 0 0 1 0 1 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --EDEMA --EXOSTOSES --FIBROSIS --INFLAMMATION, ACUTE 0110000000 0110010100 0100010100 0110000000 533 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 21 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 0 1 0 0 0 1 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --FIBROSIS --HEMORRHAGE --INFLAMMATION, CHRONIC --B-LIPOMA --I-TRANSITIONAL CELL CARCINOMA 0000001000 0000001000 0100000000 0100000000 0000010000 AORTA (AO) ........................................... NUMBER EXAMINED: 1 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 1 0 0 0 0 0 0 0 0 0 PENIS (PE) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 PREPUTIAL GLAND (PG) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 0 0 0 0 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, ACUTE --M-CARCINOMA 0000100000 0000100000 534 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 22 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 URETER (UE) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 PINNA (PN) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --HEMORRHAGE 0000000010 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 1 2 0 0 0 0 0 1 0 0 NOT REMARKABLE: 0 1 0 0 0 0 0 0 0 0 --DILATATION, SINUSOIDAL --HYPERPLASIA, LYMPHOCYTIC --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0000000100 0100000000 1000000000 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 535 TABLE 43 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifices PAGE: 23 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- SUBCUTANEOUS TIS (SQ) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 TONGUE (TO) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 0 0 0 1 0 0 0 0 0 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 1 --DILATATION, SINUSOIDAL --INFILTRATE, MACROPHAGE, PIGMENTED 0001000000 0001000000 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 1 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --B-FIBROMA 1000000000 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 0 1 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --DILATED ** END OF LIST ** 0100000000 536 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** EYE (EY) ............................................. NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 31 25 25 31 3 29 1 --FIBROSIS, CORNEAL --FIBROSIS, LENS --INFLAMMATION, ACUTE --INFLAMMATION, ACUTE, CILIARY --INFLAMMATION, ACUTE, CORNEAL --MINERALIZATION, CORNEAL --MINERALIZATION, LENS --ULCERATION, CORNEAL --VASCULARIZATION, CORNEAL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0010000 0100000 3000000 0100000 0021010 2011000 1000000 1010000 0110010 0001000 BRAIN (BR) ........................................... NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 10 12 8 11 3 11 1 --COMPRESSION, VENTRAL --DILATATION, VENTRICULAR --HEMORRHAGE --NECROSIS, GRAY MATTER --PIGMENT --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 26 16 19 22 0 18 0 21 15 17 21 0 16 0 2211040 0000010 0010010 0000010 LIVER (LI) ........................................... NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 14 13 8 4 0 6 0 --CYST 0002000 537 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 14 13 8 4 0 6 0 --DEGENERATION, CYSTIC --FIBROSIS --FIBROSIS, CAPSULAR --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERPLASIA, BILE DUCT --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) --NECROSIS, HEPATOCELLULAR --STASIS, BILE --TELANGIECTASIS --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR --B-CHOLANGIOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 3326060 0000010 0100000 5201010 1000000 0001000 2120250 5227040 0002221 8 6 7 11 0 6 0 0010000 0011000 1221331 0000010 0110000 13 8 11 16 0 10 0 1212000 0001000 1111010 LIVER, OIL RED O (LI1) ............................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 538 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- KIDNEY (KD) .......................................... NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 2 4 7 2 3 3 1 --CAST, PROTEINACEOUS --CYST --DILATATION, PELVIC --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFARCT --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --NECROSIS --NECROSIS, PAPILLARY --NEPHROPATHY, CHRONIC PROGRESSIVE --PYELONEPHRITIS --B-LIPOMA --M-NEPHROBLASTOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 10 8 10 15 0 8 0 2100000 7126030 0100000 0002000 2200010 7662090 2003010 0100000 1010010 23 15 9 13 0 15 0 4126060 0000010 0100000 1002000 SPLEEN (SP) .......................................... NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 35 28 25 26 3 27 1 --CYST, SEROSAL --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED --PIGMENT, INCREASED --B-HEMANGIOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001000 0013030 0012000 0001000 1000000 539 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- PANCREAS (PA) ........................................ NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 27 19 18 24 3 25 1 --ATROPHY, ACINAR --FIBROSIS --HEMORRHAGE --INFILTRATION, FAT, INTERSTITIAL --INFLAMMATION, VASCULAR --PIGMENT --B-ADENOMA, ISLET CELL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 3242020 0001000 1111000 0001000 0110010 1653030 3203010 1002000 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 17 16 18 17 3 21 1 --ANGIECTASIS --ATROPHY, CORTICAL --DEGENERATION, CYSTIC, --HEMORRHAGE --HYPERPLASIA, CORTICAL --HYPERTROPHY, CORTICAL --MINERALIZATION --NECROSIS, CORTICAL --ONE EXAMINED --PIGMENT --THROMBUS --VACUOLATION --M-CARCINOMA CORTICAL CELL 5104040 1000000 5253040 7217030 0100000 0202000 1000000 1000010 0001000 2010000 0001000 11 9 6 11 0 4 0 1000000 540 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 17 16 18 17 3 21 1 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1001000 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 25 23 22 29 3 28 1 --HEMORRHAGE --HYPERPLASIA, MEDULLARY --ONE EXAMINED --VACUOLATION --B-PHEOCHROMOCYTOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 2100000 1010000 0021000 0000010 9523010 1000000 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 31 24 24 30 3 29 1 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0212010 0001000 4220000 1100000 OVARY (OV) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 541 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- TESTIS (TE) .......................................... NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 30 22 21 28 3 23 1 --ATROPHY/DEGENERATION --GRANULOMA, SPERM --HEMORRHAGE --INFLAMMATION, CHRONIC --INFLAMMATION, VASCULAR --MINERALIZATION --MINERALIZATION, VASCULAR --NECROSIS --B-INTERSTITIAL CELL TUMOR 4332040 0000010 2000000 1102000 1000000 2120010 1112040 0001000 2000000 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 36 27 27 33 3 30 1 NOT REMARKABLE: 35 27 27 32 3 30 1 --OSTEODYSTROPHY, FIBROUS 1001000 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 36 27 27 33 3 30 1 NOT REMARKABLE: 35 27 27 33 3 30 1 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1000000 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 35 28 27 33 3 30 1 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1000000 542 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 36 28 27 33 3 30 1 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 36 28 27 33 3 29 1 NOT REMARKABLE: 36 28 26 33 3 27 1 --HEMORRHAGE --NECROSIS --VACUOLATION, WHITE MATTER 0000020 0000010 0010000 THYMUS (TH) .......................................... NUMBER EXAMINED: 35 28 26 31 3 30 1 NOT REMARKABLE: 30 26 24 25 0 25 1 --CYST --DEPLETION, LYMPHOCYTIC --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 3102000 0000010 3122340 1102000 LUNG (LU) ............................................ NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 19 19 12 20 2 14 1 --EDEMA, ALVEOLAR --FIBROSIS, PLEURAL/SUBPLEURAL --HEMORRHAGE --HYPERPLASIA, TYPE II PNEUMOCYTE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC 1000010 1301000 1122130 0000020 1001020 0010000 543 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LUNG (LU) ............................................ NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 19 19 12 20 2 14 1 --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, MACROPHAGE, PIGMENTED --MINERALIZATION --MINERALIZATION, VASCULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --N-ADRENAL CORTICAL CARCINOMA 11 2 4 5 0 4 0 0001010 1010000 7486090 1013000 1000000 HEART (HT) ........................................... NUMBER EXAMINED: 36 28 26 33 3 30 1 NOT REMARKABLE: 20 18 19 24 3 20 1 --DEGENERATION/NECROSIS --FIBROSIS --INFLAMMATION, CHRONIC --MINERALIZATION --OSSEOUS METAPLASIA --THROMBUS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 16 10 7 7 0 9 0 9633050 1000000 1001000 1000000 1000000 0001000 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 33 28 26 30 3 27 1 --CYST --DEGENERATION/NECROSIS --M-FIBROSARCOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0000010 1012020 1000000 1001000 544 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 36 28 27 32 3 30 1 NOT REMARKABLE: 36 28 27 32 3 30 1 TRACHEA (TR) ......................................... NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 36 28 27 33 3 30 1 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 36 28 24 33 3 30 1 NOT REMARKABLE: 36 28 24 33 3 30 1 THYROID (TY) ......................................... NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 22 13 16 19 2 21 0 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --ECTOPIC THYMUS --HEMORRHAGE --HYPERPLASIA, C-CELL --HYPERPLASIA, FOLLICULAR CELL --INFLAMMATION, GRANULOMATOUS --INFLAMMATION, VASCULAR --MINERALIZATION --ONE EXAMINED --PIGMENT --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL 1303020 6763130 0000001 1100010 0200010 0000010 1000000 0000010 1000000 0010000 0010000 7446020 0103000 545 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** THYROID (TY) ......................................... NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 22 13 16 19 2 21 0 --M-CARCINOMA, FOLLICULAR CELL 0000010 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 34 27 27 33 3 30 1 NOT REMARKABLE: 29 25 26 31 3 29 1 --HYPERPLASIA --B-ADENOMA 5112010 0100000 PITUITARY (PI) ....................................... NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 4 4 2 2 3 5 1 --CYST --HEMORRHAGE --HYPERPLASIA --PIGMENT --B-ADENOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0101000 23 15 20 22 0 17 0 0100000 0010040 32 23 25 30 0 25 0 0001000 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 36 28 27 32 3 30 1 NOT REMARKABLE: 28 22 23 30 3 27 1 --INFLAMMATION, CHRONIC 8642030 546 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 36 28 27 32 3 30 1 NOT REMARKABLE: 34 28 27 32 3 30 1 --MINERALIZATION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1000000 1000000 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 36 28 27 32 3 30 1 NOT REMARKABLE: 28 28 22 24 3 27 0 --CYST --EDEMA --EROSION --HEMORRHAGE --MINERALIZATION --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --PIGMENT --ULCERATION 0010000 1023020 5024021 0012000 3011000 0023010 1011000 0001000 1001000 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 36 28 27 32 3 30 1 NOT REMARKABLE: 30 22 23 27 3 25 1 --EDEMA --EROSION --HEMORRHAGE --INFLAMMATION, --INFLAMMATION, --ULCERATION ACUTE CHRONIC-ACTIVE 5635040 1523010 1002000 1522030 3103020 2002030 547 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 36 28 27 32 3 30 1 NOT REMARKABLE: 30 22 23 27 3 25 1 --B-PAPILLOMA, SQUAMOUS CELL 0010000 DUODENUM (DU) ........................................ NUMBER EXAMINED: 36 27 25 32 3 30 1 NOT REMARKABLE: 36 27 25 32 3 30 1 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 28 25 25 30 3 26 0 NOT REMARKABLE: 28 25 25 30 3 26 0 ILEUM (IL) ........................................... NUMBER EXAMINED: 31 25 26 31 3 30 1 NOT REMARKABLE: 30 25 26 31 3 30 1 --EDEMA 1000000 CECUM (CE) ........................................... NUMBER EXAMINED: 36 26 27 31 3 30 1 NOT REMARKABLE: 35 26 26 31 3 29 1 --EDEMA --EROSION --HEMORRHAGE --PARASITISM 1000000 1000000 1000010 0010000 548 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- COLON (CO) ........................................... NUMBER EXAMINED: 36 27 27 32 3 30 1 NOT REMARKABLE: 36 26 27 31 3 30 1 --PARASITISM 0101000 RECTUM (RE) .......................................... NUMBER EXAMINED: 36 27 26 33 3 30 1 NOT REMARKABLE: 34 26 26 32 3 30 1 --PARASITISM --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 2100000 0001000 SKIN (SK) ............................................ NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 34 22 26 29 3 26 1 --CYST, EPIDERMAL INCLUSION --EDEMA --HEMORRHAGE --INFLAMMATION, ACUTE --NECROSIS --B-HEMANGIOMA --B-KERATOACANTHOMA --B-LIPOMA --B-PAPILLOMA, SQUAMOUS CELL --M-SARCOMA, UNDIFFERENTIATED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1100000 0100000 0100010 0200000 0100000 0000010 1110000 0001000 0101030 0100000 0102000 549 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 33 27 26 30 3 28 1 --CALCULUS --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001000 0000020 1112000 1000010 0111010 1000000 0000010 0001000 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 550 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- PROSTATE (PR) ........................................ NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 8 7 4 6 3 9 1 --EDEMA --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --MINERALIZATION, LUMINAL --NECROSIS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0111000 2000000 0001000 7505040 1203050 20 14 22 18 0 11 0 0022020 0001000 0001000 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 31 26 26 25 3 24 1 --EDEMA --FIBROSIS --HYPERPLASIA, EPITHELIAL --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --ONE EXAMINED --SECRETION, INCREASED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1001010 0001000 0001000 1001020 0102000 1001010 1000000 3113040 0001000 551 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 35 26 25 33 3 28 1 --HYPOSPERMIA --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE 0110020 0010000 1100000 CAUSE OF DEATH (CD) .................................. NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 0 0 0 0 0 0 0 --ADRENAL CORTICAL CARCINOMA --AUDITORY SEBACEOUS GLAND CARCINOMA --CARCINOMA --CHRONIC PROGRESSIVE NEPHROPATHY --FIBROSARCOMA --FRACTURE, HARD PALATE --HEMANGIOMA --HEPATIC NECROSIS --HEPATOCELLULAR ADENOMA --HISTIOCYTIC SARCOMA --MALIGNANT FIBROUS HISTIOSARCOMA --MALIGNANT LYMPHOMA --NECROSIS, ANAL MUSCULATURE --NEPHROBLASTOMA --PITUITARY ADENOMA --PYELONEPHRITIS --UNDETERMINED --UNDIFFERENTIATED SARCOMA 1000000 0100000 0010000 2102000 1000000 0100000 0000010 0000211 0211000 0111000 0001000 1101010 0000010 0100000 23 15 18 21 0 21 0 1010000 7456140 0100000 552 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** CAUSE OF DEATH (CD) .................................. NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 0 0 0 0 0 0 0 --UROLITHIASIS 0000010 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 3 1 0 2 0 3 0 NOT REMARKABLE: 1 0 0 1 0 0 0 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1001010 0100020 0001000 1000000 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 2 0 2 1 0 2 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0010000 0011020 1020020 1001000 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 0 0 1 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --NECROSIS, FAT --M-CARCINOMA 0000010 0010000 553 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 0 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --BACTERIAL COLONIES --FRACTURE --HEMORRHAGE --INFLAMMATION, ACUTE 0100000 0100000 0100000 0100000 HEMATO NEOPLASIA (HN) ................................ NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 35 26 26 30 3 29 1 --M-LYMPHOMA --M-MALIGNANT FIBROUS HISTIOCYTOMA --M-SARCOMA, HISTIOCYTIC 1101010 0001000 0111000 MESENTERY (MY) ....................................... NUMBER EXAMINED: 1 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --HEMORRHAGE 1000000 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 1 1 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001000 0001000 1100000 554 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 2 5 1 4 0 1 0 NOT REMARKABLE: 0 2 0 0 0 1 0 --DILATATION, SINUSOIDAL --HYPERPLASIA, LYMPHOCYTIC --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1214000 0112000 1000000 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 3 1 0 2 0 1 0 NOT REMARKABLE: 0 0 0 0 0 1 0 --DILATATION, SINUSOIDAL --FIBROSIS --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 2101000 0001000 2000000 1001000 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 14 8 6 10 0 5 0 NOT REMARKABLE: 0 0 0 2 0 0 0 --GALACTOCELE --HEMORRHAGE --MINERALIZATION --PIGMENT --B-FIBROADENOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 12 5 4 4 0 2 0 1000000 1200000 1223030 1100000 0001000 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 2 0 0 0 0 1 0 NOT REMARKABLE: 2 0 0 0 0 1 0 555 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 20 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 TAIL (TI) ............................................ NUMBER EXAMINED: 2 3 1 2 0 3 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --CYST, EPIDERMAL INCLUSION --EROSION --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --NECROSIS --B-KERATOACANTHOMA 1211010 2200010 1000000 0001000 2201010 0000010 0100000 ANUS (AN) ............................................ NUMBER EXAMINED: 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --NECROSIS, MUSCULAR 0000010 FOOT/FOOTPAD(S) (FP) ................................. NUMBER EXAMINED: 9 2 6 5 0 6 0 NOT REMARKABLE: 1 0 0 0 0 0 0 --EDEMA --EROSION --EXOSTOSES --FIBROSIS --HEMORRHAGE 3032020 6265040 2002020 2263030 0001000 556 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 21 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** FOOT/FOOTPAD(S) (FP) ................................. NUMBER EXAMINED: 9 2 6 5 0 6 0 NOT REMARKABLE: 1 0 0 0 0 0 0 --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC ACTIVE --PIGMENT 7255050 1010010 0000010 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 1 0 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --DISLOCATION --EDEMA --EXOSTOSES --HEMORRHAGE --INFLAMMATION, --INFLAMMATION, CHRONIC CHRONIC-ACTIVE, SYNOVIAL 1000000 1000000 0001000 1000000 0001000 1000000 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 1 0 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC ACTIVE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1000000 0001000 557 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 22 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- AORTA (AO) ........................................... NUMBER EXAMINED: 2 0 1 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --MINERALIZATION 2010000 PENIS (PE) ........................................... NUMBER EXAMINED: 1 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --EDEMA --HEMORRHAGE --INFLAMMATION, CHRONIC ACTIVE --PROLAPSE 1000000 1000000 1000000 1100000 PREPUTIAL GLAND (PG) ................................. NUMBER EXAMINED: 0 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC 0100000 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 0 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --M-CARCINOMA 0100000 AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 1 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --MINERALIZATION 1000000 558 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 23 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 1 0 0 2 0 0 0 NOT REMARKABLE: 1 0 0 0 0 0 0 --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001000 0001000 URETER (UE) .......................................... NUMBER EXAMINED: 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --DILATATION --INFLAMMATION, CHRONIC 0000010 0000010 PINNA (PN) ........................................... NUMBER EXAMINED: 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 1 0 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 0 0 1 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --DILATATION, SINUSOIDAL --HYPERPLASIA, LYMPHOCYTIC 0010000 0010000 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 559 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 24 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- SUBCUTANEOUS TIS (SQ) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 TONGUE (TO) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 560 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 25 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** EYE (EY) ............................................. NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 37 36 35 26 0 26 0 --INFLAMMATION, ACUTE --INFLAMMATION, ACUTE, CORNEAL --INFLAMMATION, CHRONIC-ACTIVE, --MINERALIZATION, CORNEAL --ONE EXAMINED --SYNECHIA, ANTERIOR --ULCERATION, CORNEAL --VASCULARIZATION, CORNEAL CORNEAL 0001000 0001000 0000010 0100000 0001000 0000010 0001000 0001020 BRAIN (BR) ........................................... NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 4 4 6 9 0 9 0 --COMPRESSION, VENTRAL --DILATATION, VENTRICULAR --HEMORRHAGE --PIGMENT --VACUOLATION, WHITE MATTER --M-ASTROCYTOMA --I-PITUITARY CARCINOMA 32 32 29 20 0 18 0 22 24 21 13 0 12 0 4773020 0010000 0000010 1000000 0000010 LIVER (LI) ........................................... NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 18 15 19 9 0 11 0 --CYST --FIBROSIS 1001000 0310000 561 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 26 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 18 15 19 9 0 11 0 --FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERPLASIA, BILE DUCT --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --MINERALIZATION --NECROSIS, HEPATOCELLULAR --NECROSIS, INDIVIDUAL HEPATOCYTE --PIGMENT --PIGMENT, HEPATOCELLULAR --PIGMENT, KUPFFER CELL --TELANGIECTASIS --THROMBUS --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001000 3301010 0010000 1103010 2130020 3235000 6 10 6 8 0 7 0 0100000 1000000 4434020 0001000 0100000 0000010 0001000 2410010 0000010 9 8 6 11 0 6 0 0021010 0000010 LIVER, OIL RED O (LI1) ............................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 562 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- KIDNEY (KD) .......................................... NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 4 1 8 10 0 3 0 --CAST, PROTEINACEOUS --CYST --DILATATION, PELVIC --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFARCT --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --NEPHROPATHY, CHRONIC PROGRESSIVE --PYELONEPHRITIS --B-LIPOMA 9 12 11 9 0 8 0 3001000 4230020 0101000 0100010 0000010 23 24 18 14 0 19 0 7741030 8622030 1212020 0000010 SPLEEN (SP) .......................................... NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 30 32 28 22 0 21 0 --FIBROSIS, CAPSULAR --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED --PIGMENT, INCREASED --B-HEMANGIOMA 0100000 5226060 2151010 0100000 PANCREAS (PA) ........................................ NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 31 35 31 25 0 26 0 --ATROPHY, ACINAR --HYPERPLASIA, ISLET CELLS 4123010 1000000 563 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 28 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** PANCREAS (PA) ........................................ NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 31 35 31 25 0 26 0 --PIGMENT --B-ADENOMA, ISLET CELL --I-ENDOMETRIAL STROMAL SARCOMA 2021000 0000010 0100000 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 5 3 2 14 0 3 0 --ANGIECTASIS --DEGENERATION, CYSTIC, CORTICAL --FIBROSIS --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERTROPHY, CORTICAL CELL --MINERALIZATION --PIGMENT --THROMBUS --VACUOLATION --B-ADENOMA 25 30 23 9 0 15 0 11 15 16 5 0 13 0 0001000 0000010 31 33 30 10 0 18 0 2020000 0100010 3341010 2321030 3224010 2022010 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 37 35 34 27 0 27 0 --ONE EXAMINED --VACUOLATION --B-PHEOCHROMOCYTOMA 0001000 0001010 0210010 564 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 29 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 36 36 35 28 0 28 0 NOT REMARKABLE: 33 34 29 26 0 25 0 --DILATATION, SINUSOIDAL --EDEMA --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 0002000 0010000 1011010 3260020 OVARY (OV) ........................................... NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 26 25 26 16 0 21 0 --CYST --HEMORRHAGE --MINERALIZATION --ONE EXAMINED --PIGMENTED 7898070 1202000 2201000 1102000 0001000 TESTIS (TE) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 37 37 35 29 0 27 0 NOT REMARKABLE: 37 37 35 29 0 27 0 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 37 37 35 29 0 27 0 NOT REMARKABLE: 37 37 35 29 0 27 0 565 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 30 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 37 37 35 29 0 28 0 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 37 37 35 29 0 28 0 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 37 37 34 29 0 27 0 --HEMORRHAGE 0010010 THYMUS (TH) .......................................... NUMBER EXAMINED: 37 36 35 28 0 25 0 NOT REMARKABLE: 23 27 24 17 0 19 0 --CYST --DEPLETION, LYMPHOCYTIC --HEMORRHAGE --M-THYMIC CARCINOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 10 7 10 9 0 5 0 1010000 3200010 0001000 1001000 LUNG (LU) ............................................ NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 28 29 26 18 0 14 0 --EDEMA, ALVEOLAR --FOREIGN BODY --HEMORRHAGE 0100010 0010000 0120030 566 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 31 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LUNG (LU) ............................................ NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 28 29 26 18 0 14 0 --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --INFLAMMATION, GRANULOMATOUS --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, MACROPHAGE, PIGMENTED --MINERALIZATION --MINERALIZATION, VASCULAR --THROMBUS --N-MAMMARY CARCINOMA 0002020 0110000 0010000 5045060 0010000 0201030 6423060 0001000 0001010 HEART (HT) ........................................... NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 30 32 30 27 0 28 0 --DEGENERATION/NECROSIS --FIBROSIS --MINERALIZATION 7542000 3132000 0011000 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 36 37 34 29 0 28 0 --DEGENERATION/NECROSIS 1010000 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 36 37 35 29 0 28 0 NOT REMARKABLE: 36 37 35 29 0 28 0 567 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 32 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- TRACHEA (TR) ......................................... NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 37 37 35 29 0 28 0 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 37 37 35 29 0 28 0 THYROID (TY) ......................................... NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 23 19 26 19 0 22 0 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --HEMORRHAGE --HYPERPLASIA, C-CELL --INFLAMMATION, CHRONIC --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR --M-CARCINOMA, C-CELL CELL 1101000 8962020 0311000 1010000 0000010 5946030 1002010 1000000 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 34 34 34 28 0 27 0 NOT REMARKABLE: 34 34 34 28 0 27 0 PITUITARY (PI) ....................................... NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 3 1 1 2 0 2 0 --CYST 2442000 568 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 33 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** PITUITARY (PI) ....................................... NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 3 1 1 2 0 2 0 --HEMORRHAGE --HYPERPLASIA --NECROSIS --PIGMENT --B-ADENOMA --M-CARCINOMA 25 29 30 17 0 18 0 0000010 0100000 3675030 34 35 34 26 0 24 0 0000010 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 28 31 27 24 0 26 0 --INFLAMMATION, CHRONIC --ONE EXAMINED 8685020 1000000 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 37 37 35 28 0 27 0 NOT REMARKABLE: 37 37 35 28 0 27 0 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 35 36 32 24 0 27 0 --CYST --EDEMA --EROSION --MINERALIZATION --INFLAMMATION, ACUTE 0000010 1013000 1122000 0011000 0010000 569 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 34 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 30 32 32 25 0 23 0 --EDEMA --EROSION --FIBROSIS --HEMORRHAGE --HYPERKERATOSIS, NONGLANDULAR --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION MUCOSA 6534040 1302020 1000000 1002000 1000000 2404020 3000000 2002010 DUODENUM (DU) ........................................ NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 37 37 35 29 0 28 0 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 36 37 33 28 0 26 0 NOT REMARKABLE: 35 36 33 28 0 25 0 --CYST, KERATIN --DIVERTICULUM --B-ANGIOMA 0000010 0100000 1000000 ILEUM (IL) ........................................... NUMBER EXAMINED: 34 36 34 29 0 27 0 NOT REMARKABLE: 34 36 34 29 0 27 0 570 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 35 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- CECUM (CE) ........................................... NUMBER EXAMINED: 36 37 35 29 0 28 0 NOT REMARKABLE: 35 37 35 29 0 28 0 --PARASITISM 1000000 COLON (CO) ........................................... NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 36 37 35 28 0 28 0 --EDEMA --PARASITISM 0001000 1000000 RECTUM (RE) .......................................... NUMBER EXAMINED: 37 37 35 29 0 27 0 NOT REMARKABLE: 36 35 32 29 0 27 0 --PARASITISM 1230000 SKIN (SK) ............................................ NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 32 33 27 25 0 22 0 --ACUTE INFLAMMATORY EXUDATE --ATROPHY, ADNEXAL --CYST, EPIDERMAL INCLUSION --EDEMA --FAT NECROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE 0100000 0001010 1000000 0010010 1000000 1111000 3231020 0011000 0001020 571 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 36 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SKIN (SK) ............................................ NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 32 33 27 25 0 22 0 --ULCERATION --B-FIBROMA --B-KERATOACANTHOMA --B-LIPOMA --B-PAPILLOMA, SQUAMOUS CELL --M-FIBROSARCOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 3242050 0010000 0010000 1000000 0000010 0110000 0000010 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 5 5 7 7 0 4 0 --EDEMA --GALACTOCELE --HEMORRHAGE --HYPERPLASIA, GLANDULAR --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --MINERALIZATION --NECROSIS --PIGMENT --B-ADENOMA --B-FIBROADENOMA --M-CARCINOMA --M-FIBROSARCOMA 0000010 23 25 24 16 0 16 0 4357020 1000000 0143000 0010000 0010010 5785030 1232000 3253000 2322040 14 13 11 6 0 8 0 8 7 10 7 0 4 0 1000000 572 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 37 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 5 5 7 7 0 4 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0000010 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 37 37 35 27 0 28 0 --INFLAMMATION, CHRONIC 0002000 CERVIX (CV) .......................................... NUMBER EXAMINED: 37 37 34 29 0 27 0 NOT REMARKABLE: 36 35 33 29 0 24 0 --DILATATION --HEMORRHAGE --INFLAMMATION, ACUTE --NECROSIS --B-POLYP, ENDOMETRIAL STROMAL --M-SARCOMA, ENDOMETRIAL STROMAL 1100010 0100010 0000010 0100000 1100030 0110000 UTERUS (UT) .......................................... NUMBER EXAMINED: 37 37 34 29 0 28 0 NOT REMARKABLE: 30 27 28 21 0 22 0 --DILATATION --HEMORRHAGE --HYPERPLASIA, CYSTIC, --INFLAMMATION, ACUTE --NECROSIS ENDOMETRIAL 6845050 0002010 0212010 0011010 0000010 573 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 38 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** UTERUS (UT) .......................................... NUMBER EXAMINED: 37 37 34 29 0 28 0 NOT REMARKABLE: 30 27 28 21 0 22 0 --PIGMENT --B-POLYP, ENDOMETRIAL, STROMAL --M-HEMANGIOSARCOMA 0001000 1011010 1000000 VAGINA (VA) .......................................... NUMBER EXAMINED: 37 37 35 28 0 27 0 NOT REMARKABLE: 37 35 33 28 0 27 0 --HEMORRHAGE --B-PAPILLOMA, SQUAMOUS CELL --M-CARCINOMA, SQUAMOUS CELL --I-ENDOMETRIAL STROMAL SARCOMA 0100000 0100000 0010000 0010000 PROSTATE (PR) ........................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 574 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 39 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- CAUSE OF DEATH (CD) .................................. NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --ASTROCYTOMA --C-CELL CARCINOMA --CHRONIC PROGRESSIVE NEPHROPATHY --ENDOMETRIAL STROMAL POLYP --ENDOMETRIAL STROMAL SARCOMA --FIBROMA --FIBROSARCOMA --HEMANGIOSARCOMA --HEPATOCELLULAR ADENOMA --KERATIN CYST --KERATOACANTHOMA --LIPOMA --LIPOSARCOMA --MALIGNANT FIBROUS HISTIOSARCOMA --MALIGNANT LYMPHOMA --MAMMARY CARCINOMA --MAMMARY FIBROADENOMA --PITUITARY ADENOMA --PITUITARY CARCINOMA --RENAL MINERALIZATION --SQUAMOUS CELL CARCINOMA --THYMIC CARCINOMA --UNDETERMINED --UTERINE HEMORRHAGE 1000000 1000000 0000010 0000020 0110000 0010000 0110000 1000000 0010000 0000010 0010000 1000000 0010000 0000010 1000000 0363020 5401010 27 28 22 16 0 18 0 0001010 0001000 0010000 0001000 0005010 0001000 575 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 40 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 4 3 5 7 0 5 0 NOT REMARKABLE: 2 2 3 4 0 3 0 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC 1012000 1111020 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 2 2 5 3 0 4 0 NOT REMARKABLE: 0 0 1 0 0 1 0 --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 1211030 2143020 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 0 4 0 2 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --HEMORRHAGE --INFLAMMATION, CHRONIC --MINERALIZATION --NECROSIS, FAT --PIGMENT --THROMBUS --I-ENDOMETRIAL STROMAL SARCOMA 0101000 0001000 0101000 0302010 0001000 0001000 0100000 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 576 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 41 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- HEMATO NEOPLASIA (HN) ................................ NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 36 37 35 28 0 27 0 --M-LYMPHOMA --M-MALIGNANT FIBROUS HISTIOCYTOMA 1001000 0000010 MESENTERY (MY) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 2 2 2 1 0 4 0 NOT REMARKABLE: 1 1 1 1 0 1 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --N-ENDOMETRIAL STROMAL SARCOMA 1000010 1100010 0000020 0010000 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 0 1 0 1 0 1 0 NOT REMARKABLE: 0 1 0 0 0 0 0 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED 0001000 0000010 0001000 577 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 42 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 12 13 12 6 0 3 0 NOT REMARKABLE: 8 9 8 2 0 1 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001000 3413000 1021010 1111000 0000010 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 0 1 0 0 0 0 0 NOT REMARKABLE: 0 1 0 0 0 0 0 TAIL (TI) ............................................ NUMBER EXAMINED: 0 0 1 2 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --CYST, EPIDERMAL INCLUSION --EROSION --INFLAMMATION, ACUTE 0002000 0010000 0012000 ANUS (AN) ............................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 578 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 43 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- FOOT/FOOTPAD(S) (FP) ................................. NUMBER EXAMINED: 4 3 3 0 0 2 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --CYST, EPIDERMAL INCLUSION --EDEMA --EROSION --EXOSTOSES --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC ACTIVE 0100000 1100010 4200010 1100000 3230010 0100000 4320020 0010000 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 3 3 3 1 0 0 0 NOT REMARKABLE: 1 2 2 0 0 0 0 --HYPERPLASIA, LYMPHOCYTIC --N-MAMMARY CARCINOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1110000 0001000 1000000 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 2 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --EXOSTOSES --INFLAMMATION, CHRONIC 2000000 2000000 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 579 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 44 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- AORTA (AO) ........................................... NUMBER EXAMINED: 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --MINERALIZATION 0000010 PENIS (PE) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 PREPUTIAL GLAND (PG) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 URETER (UE) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 580 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 45 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- PINNA (PN) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 0 0 1 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --M-LIPOSARCOMA 0010000 SUBCUTANEOUS TIS (SQ) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 TONGUE (TO) .......................................... NUMBER EXAMINED: 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --CYST 0000010 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 581 TABLE 44 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 46 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 582 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** EYE (EY) ............................................. NUMBER EXAMINED: 65 34 33 65 8 30 1 NOT REMARKABLE: 58 30 31 62 8 29 1 --CATARACT --FIBROSIS, CORNEAL --FIBROSIS, LENS --HYPERPLASIA, CORNEAL EPITHELIUM --INFLAMMATION, ACUTE --INFLAMMATION, ACUTE, CILIARY --INFLAMMATION, ACUTE, CORNEAL --MINERALIZATION, CORNEAL --MINERALIZATION, LENS --ULCERATION, CORNEAL --VASCULARIZATION, CORNEAL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1000000 0010000 0100000 1000000 3000000 0200000 1122010 3011000 1000000 1110000 0210010 0001000 BRAIN (BR) ........................................... NUMBER EXAMINED: 65 37 36 65 8 34 1 NOT REMARKABLE: 37 17 15 39 8 11 1 --COMPRESSION, VENTRAL --DILATATION, VENTRICULAR --HEMORRHAGE --NECROSIS, GRAY MATTER --PIGMENT --B-GRANULAR CELL TUMOR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 28 20 21 25 0 22 0 21 16 17 22 0 16 0 2211040 0000010 0010010 0001000 0000010 583 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LIVER (LI) ........................................... NUMBER EXAMINED: 65 55 55 65 8 40 1 NOT REMARKABLE: 26 17 10 5 0 8 0 --CYST --DEGENERATION, CYSTIC --FIBROSIS --FIBROSIS, CAPSULAR --FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERPLASIA, BILE DUCT --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) --NECROSIS, HEPATOCELLULAR --PIGMENT --STASIS, BILE --TELANGIECTASIS --THROMBUS --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR --B-CHOLANGIOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1002000 6 8 14 18 0 7 0 0010020 0100000 1110000 8 12 11 6 0 2 0 1021000 1001000 3331570 9 9 8 11 0 5 0 0 1 3 22 7 2 1 0000010 18 20 23 20 0 9 0 0010000 0212000 1342641 0111010 0001010 2730000 0100000 17 18 23 39 1 11 0 2425000 0001000 2111010 584 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LIVER, OIL RED O (LI1) ............................... NUMBER EXAMINED: 5 0 0 5 0 0 0 NOT REMARKABLE: 1 0 0 1 0 0 0 --POSITIVE OIL RED O STAINING 4004000 KIDNEY (KD) .......................................... NUMBER EXAMINED: 65 55 55 65 8 40 1 NOT REMARKABLE: 8 7 13 20 7 3 1 --CAST, PROTEINACEOUS --CYST --DEGENERATION, TUBULAR --DILATATION, PELVIC --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFARCT --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --MINERALIZATION --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --NECROSIS --NECROSIS, PAPILLARY --NEPHROPATHY, CHRONIC PROGRESSIVE --PYELONEPHRITIS --THROMBUS --B-LIPOMA --M-LIPOSARCOMA --M-NEPHROBLASTOMA 17 15 16 18 0 11 0 4530100 0100000 8267050 0100000 1002000 2410020 1100000 0101000 1000000 9 8 10 4 0 9 0 2003010 0100000 1010010 37 29 24 22 1 22 0 5226060 1000000 0000010 0000010 0100000 585 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** KIDNEY (KD) .......................................... NUMBER EXAMINED: 65 55 55 65 8 40 1 NOT REMARKABLE: 8 7 13 20 7 3 1 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1002000 SPLEEN (SP) .......................................... NUMBER EXAMINED: 65 34 33 65 8 30 1 NOT REMARKABLE: 63 34 31 58 8 27 1 --CYST, SEROSAL --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED --PIGMENT, INCREASED --B-HEMANGIOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001000 0013030 0012000 0001000 2000000 PANCREAS (PA) ........................................ NUMBER EXAMINED: 65 55 55 65 8 40 1 NOT REMARKABLE: 45 32 36 45 8 28 1 --ATROPHY, ACINAR --CYST --FIBROSIS --HEMORRHAGE --HYPERPLASIA, ACINAR --HYPERTROPHY, VASCULAR --INFILTRATE, EOSINOPHILS --INFILTRATION, FAT, INTERSTITIAL --INFLAMMATION, CHRONIC --INFLAMMATION, VASCULAR --PIGMENT 10 9 10 9 0 7 0 0100000 1002000 3211000 1200000 2000010 1000000 0001000 2001000 2310020 2896050 586 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** PANCREAS (PA) ........................................ NUMBER EXAMINED: 65 55 55 65 8 40 1 NOT REMARKABLE: 45 32 36 45 8 28 1 --B-ADENOMA, ISLET CELL --M-CARCINOMA, ISLET CELL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 4726020 0010000 1002000 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 65 35 36 65 8 30 1 NOT REMARKABLE: 30 18 25 40 8 21 1 --ADRENAL TISSUE, ECTOPIC --ANGIECTASIS --ATROPHY, CORTICAL --DEGENERATION, CYSTIC, CORTICAL --HEMORRHAGE --HYPERPLASIA, CORTICAL --HYPERTROPHY, CORTICAL CELL --MINERALIZATION --NECROSIS, CORTICAL --ONE EXAMINED --PIGMENT --THROMBUS --VACUOLATION --M-CARCINOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001000 9205040 1000000 9364040 14 4 1 8 0 3 0 3101000 3202000 2000000 1000010 0001000 2110000 2101000 24 13 7 19 0 4 0 1000000 2001000 587 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 65 34 36 65 8 31 1 NOT REMARKABLE: 54 28 28 59 8 28 1 --HEMORRHAGE --HYPERPLASIA, MEDULLARY --NECROSIS --ONE EXAMINED --VACUOLATION --B-PHEOCHROMOCYTOMA --M-MALIGNANT PHEOCHROMOCYTOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 2101000 1010000 0010000 0021000 0000010 9645010 0010010 1000000 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 65 34 35 65 8 30 1 NOT REMARKABLE: 55 29 30 59 8 29 1 --DILATATION, SINUSOIDAL --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --NECROSIS --THROMBUS --M-HEMANGIOSARCOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1001000 1334010 0001000 4331000 1000000 1000000 1100000 2101000 OVARY (OV) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 588 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- TESTIS (TE) .......................................... NUMBER EXAMINED: 65 55 55 65 8 40 1 NOT REMARKABLE: 57 43 44 52 8 32 1 --ATROPHY/DEGENERATION --GRANULOMA, SPERM --HEMORRHAGE --INFLAMMATION, CHRONIC --INFLAMMATION, VASCULAR --MINERALIZATION --MINERALIZATION, VASCULAR --NECROSIS --B-INTERSTITIAL CELL TUMOR 5676040 0000010 2011000 2412000 1000000 2232010 1114050 0001000 3131000 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 55 27 28 60 3 30 1 NOT REMARKABLE: 54 27 28 59 3 30 1 --OSTEODYSTROPHY, FIBROUS 1001000 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 55 27 28 60 3 30 1 NOT REMARKABLE: 54 27 28 60 3 30 1 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1000000 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 55 28 28 60 3 30 1 NOT REMARKABLE: 54 28 28 60 3 30 1 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1000000 589 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 55 28 28 60 3 30 1 NOT REMARKABLE: 55 28 28 60 3 30 1 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 55 28 28 60 3 29 1 NOT REMARKABLE: 55 28 27 60 3 27 1 --HEMORRHAGE --NECROSIS --VACUOLATION, WHITE MATTER 0000020 0000010 0010000 THYMUS (TH) .......................................... NUMBER EXAMINED: 55 28 27 57 3 30 1 NOT REMARKABLE: 47 26 25 51 0 25 1 --CYST --DEPLETION, LYMPHOCYTIC --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 5102000 0000010 4122340 1102000 LUNG (LU) ............................................ NUMBER EXAMINED: 55 50 50 60 3 40 1 NOT REMARKABLE: 33 33 23 38 2 18 1 --EDEMA, ALVEOLAR --FIBROSIS, PLEURAL/SUBPLEURAL --HEMORRHAGE --HYPERPLASIA, MEDIAL, ARTERIAL/ARTERIOLAR --HYPERPLASIA, TYPE II PNEUMOCYTE --INFLAMMATION, ACUTE 1000010 2321020 2132130 0001000 0002020 1001020 590 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LUNG (LU) ............................................ NUMBER EXAMINED: 55 50 50 60 3 40 1 NOT REMARKABLE: 33 33 23 38 2 18 1 --INFLAMMATION, CHRONIC --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, MACROPHAGE, PIGMENTED --MINERALIZATION --MINERALIZATION, VASCULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --N-ADRENAL CORTICAL CARCINOMA 2030000 15 3 5 7 0 7 0 0001010 1111010 8 11 15 10 0 13 0 1013000 1000000 HEART (HT) ........................................... NUMBER EXAMINED: 55 29 27 60 3 30 1 NOT REMARKABLE: 34 18 20 48 3 20 1 --DEGENERATION/NECROSIS --FIBROSIS --INFLAMMATION, CHRONIC --MINERALIZATION --OSSEOUS METAPLASIA --THROMBUS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 20 11 7 10 0 9 0 13 7 3 3 0 5 0 1000000 1001000 1000000 1000000 0001000 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 55 29 28 60 3 30 1 NOT REMARKABLE: 52 28 26 57 3 27 1 --CYST --DEGENERATION/NECROSIS --HEMORRHAGE 0000010 1112020 0010000 591 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 55 29 28 60 3 30 1 NOT REMARKABLE: 52 28 26 57 3 27 1 --M-FIBROSARCOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1000000 1001000 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 55 28 28 59 3 30 1 NOT REMARKABLE: 55 28 28 59 3 30 1 TRACHEA (TR) ......................................... NUMBER EXAMINED: 55 28 28 60 3 30 1 NOT REMARKABLE: 55 28 28 60 3 30 1 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 55 28 25 60 3 30 1 NOT REMARKABLE: 55 28 25 60 3 30 1 THYROID (TY) ......................................... NUMBER EXAMINED: 55 50 50 60 3 40 1 NOT REMARKABLE: 31 21 25 30 2 23 0 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --ECTOPIC THYMUS --HEMORRHAGE --HYPERPLASIA, C-CELL --HYPERPLASIA, FOLLICULAR CELL --INFLAMMATION, GRANULOMATOUS 2448040 10 15 11 9 1 5 0 0000001 1201010 3320030 0002010 1100000 592 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** THYROID (TY) ......................................... NUMBER EXAMINED: 55 50 50 60 3 40 1 NOT REMARKABLE: 31 21 25 30 2 23 0 --INFLAMMATION, VASCULAR --MINERALIZATION --NECROSIS --ONE EXAMINED --PIGMENT --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL --M-CARCINOMA, C-CELL --M-CARCINOMA, FOLLICULAR CELL 0000010 1001000 0010000 0010000 1010000 10 10 10 12 0 5 0 0316010 0010000 0010010 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 54 29 27 58 3 30 1 NOT REMARKABLE: 46 26 26 56 3 29 1 --HYPERPLASIA --B-ADENOMA 8212010 0100000 PITUITARY (PI) ....................................... NUMBER EXAMINED: 55 33 38 60 3 36 1 NOT REMARKABLE: 8 4 2 8 3 5 1 --CYST --HEMORRHAGE --HYPERPLASIA --PIGMENT --B-ADENOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1122000 29 18 25 31 0 23 0 1103000 0010040 46 28 36 48 0 31 0 0001000 593 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 55 28 28 59 3 30 1 NOT REMARKABLE: 46 22 24 53 3 27 1 --INFLAMMATION, CHRONIC --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 8646030 1000000 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 55 28 28 59 3 30 1 NOT REMARKABLE: 53 28 28 59 3 30 1 --MINERALIZATION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1000000 1000000 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 57 31 29 59 3 31 1 NOT REMARKABLE: 46 29 23 48 3 27 0 --CYST --EDEMA --EROSION --FIBROSIS --HEMORRHAGE --MINERALIZATION --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --PIGMENT --ULCERATION 0010000 3235030 5036031 0010000 0012000 4111000 0023010 1021000 0001000 1001000 594 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 57 29 29 59 3 30 1 NOT REMARKABLE: 50 22 24 54 3 25 1 --EDEMA --EROSION --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --B-PAPILLOMA, SQUAMOUS CELL 6745040 2533010 2002000 1622030 3103020 2102030 0010000 DUODENUM (DU) ........................................ NUMBER EXAMINED: 59 27 26 59 4 30 1 NOT REMARKABLE: 59 27 26 59 4 30 1 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 48 25 26 57 3 26 0 NOT REMARKABLE: 48 25 26 57 3 26 0 ILEUM (IL) ........................................... NUMBER EXAMINED: 50 25 27 58 3 30 1 NOT REMARKABLE: 49 25 27 58 3 30 1 --EDEMA 1000000 CECUM (CE) ........................................... NUMBER EXAMINED: 55 26 29 58 3 30 1 NOT REMARKABLE: 54 26 28 58 3 29 1 --EDEMA 1000000 595 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** CECUM (CE) ........................................... NUMBER EXAMINED: 55 26 29 58 3 30 1 NOT REMARKABLE: 54 26 28 58 3 29 1 --EROSION --HEMORRHAGE --PARASITISM 1000000 1000010 0010000 COLON (CO) ........................................... NUMBER EXAMINED: 55 27 28 59 3 30 1 NOT REMARKABLE: 55 26 28 57 3 30 1 --PARASITISM 0102000 RECTUM (RE) .......................................... NUMBER EXAMINED: 55 27 27 59 3 30 1 NOT REMARKABLE: 50 26 27 53 3 30 1 --PARASITISM --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 5105000 0001000 SKIN (SK) ............................................ NUMBER EXAMINED: 55 35 33 60 3 32 1 NOT REMARKABLE: 51 23 27 52 3 26 1 --ACUTE INFLAMMATORY EXUDATE --CYST, EPIDERMAL INCLUSION --EDEMA --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --NECROSIS 0000010 2110000 0100000 1111020 0420000 0000010 0100000 596 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SKIN (SK) ............................................ NUMBER EXAMINED: 55 35 33 60 3 32 1 NOT REMARKABLE: 51 23 27 52 3 26 1 --ULCERATION --B-FIBROMA --B-HEMANGIOMA --B-KERATOACANTHOMA --B-LIPOMA --B-PAPILLOMA, SQUAMOUS CELL --M-CARCINOMA, SQUAMOUS CELL --M-SARCOMA, UNDIFFERENTIATED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0220020 1110000 0000010 1251000 0103000 0302030 0100000 0100000 0102000 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 55 50 50 60 3 40 1 NOT REMARKABLE: 51 37 39 56 3 35 1 --CALCULUS --DESQUAMATION, TRANSITIONAL EPITHELIUM --EDEMA --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE 0 10 8 1 0 3 0 0010000 0010000 0000020 1222000 1000010 0222010 1110000 597 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 55 50 50 60 3 40 1 NOT REMARKABLE: 51 37 39 56 3 35 1 --ULCERATION --B-PAPILLOMA, TRANSITIONAL CELL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0000010 1000000 0001000 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 PROSTATE (PR) ........................................ NUMBER EXAMINED: 55 31 33 60 3 31 1 NOT REMARKABLE: 13 7 5 22 3 9 1 --EDEMA --FIBROSIS --HEMORRHAGE --INFLAMMATION, --INFLAMMATION, --INFLAMMATION, ACUTE CHRONIC CHRONIC-ACTIVE 0111000 2000000 0001000 9627040 4214060 28 15 24 26 0 11 0 598 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** PROSTATE (PR) ........................................ NUMBER EXAMINED: 55 31 33 60 3 31 1 NOT REMARKABLE: 13 7 5 22 3 9 1 --MINERALIZATION, LUMINAL --NECROSIS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0124020 0001000 1001000 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 55 28 28 60 3 30 1 NOT REMARKABLE: 48 26 27 51 3 24 1 --EDEMA --FIBROSIS --HYPERPLASIA, EPITHELIAL --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --ONE EXAMINED --SECRETION, INCREASED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1001010 0001000 0001000 1001020 0102000 1002010 1000000 4113040 1001000 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 56 28 28 60 3 30 1 NOT REMARKABLE: 53 26 25 58 3 28 1 --HYPOSPERMIA --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1112020 0020000 1100000 1000000 599 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- CAUSE OF DEATH (CD) .................................. NUMBER EXAMINED: 70 60 60 70 70 40 40 NOT REMARKABLE: 0 0 0 0 0 0 0 --SCHEDULED SACRIFICE --ADRENAL CORTICAL CARCINOMA --AUDITORY SEBACEOUS GLAND CARCINOMA --CARCINOMA --CHRONIC PROGRESSIVE NEPHROPATHY --FIBROSARCOMA --FRACTURE, HARD PALATE --HEMANGIOMA --HEPATIC NECROSIS --HEPATOCELLULAR ADENOMA --HISTIOCYTIC SARCOMA --MALIGNANT FIBROUS HISTIOSARCOMA --MALIGNANT LYMPHOMA --NECROSIS, ANAL MUSCULATURE --NEPHROBLASTOMA --PITUITARY ADENOMA --PYELONEPHRITIS --TRAUMA --UNDETERMINED --UNDIFFERENTIATED SARCOMA --UROLITHIASIS 34 32 32 37 67 10 39 1000000 0100000 0010000 2102000 1000000 0100000 0000010 0000211 0211000 0111000 0001000 1101010 0000010 0100000 23 15 18 21 0 21 0 1010000 0010000 7456140 0100000 0000010 600 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 7 8 3 5 0 5 0 NOT REMARKABLE: 1 2 0 1 0 0 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0101020 4533020 0101030 0001000 2000000 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 4 1 3 3 0 2 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1122000 1011020 1020020 1001000 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 0 0 1 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --NECROSIS, FAT --M-CARCINOMA 0000010 0010000 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 0 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --BACTERIAL COLONIES --FRACTURE 0100000 0100000 601 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 20 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 0 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --HEMORRHAGE --INFLAMMATION, ACUTE 0100000 0100000 HEMATO NEOPLASIA (HN) ................................ NUMBER EXAMINED: 55 28 28 60 3 30 1 NOT REMARKABLE: 53 26 27 56 3 29 1 --M-LYMPHOMA --M-MALIGNANT FIBROUS HISTIOCYTOMA --M-SARCOMA, HISTIOCYTIC 2102010 0001000 0111000 MESENTERY (MY) ....................................... NUMBER EXAMINED: 1 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --HEMORRHAGE 1000000 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 1 2 1 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --N-ISLET CELL CARCINOMA 0010000 0111000 0001000 1100000 0010000 602 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 21 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 4 9 4 4 0 1 0 NOT REMARKABLE: 0 2 0 0 0 1 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 3634000 0010000 0122000 1000000 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 3 1 1 2 0 1 0 NOT REMARKABLE: 0 0 0 0 0 1 0 --DILATATION, SINUSOIDAL --FIBROSIS --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 2101000 0001000 2010000 1001000 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 17 9 6 13 0 6 0 NOT REMARKABLE: 0 0 0 2 0 0 0 --GALACTOCELE --HEMORRHAGE --MINERALIZATION --NECROSIS --PIGMENT --B-ADENOMA --B-FIBROADENOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 12 6 4 5 0 3 0 1000000 1200010 1000000 2224030 0001000 3100000 1001000 603 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 22 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 2 3 0 3 0 1 0 NOT REMARKABLE: 2 2 0 2 0 1 0 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC 0001000 0100000 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 TAIL (TI) ............................................ NUMBER EXAMINED: 2 4 1 3 0 3 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --CYST, EPIDERMAL INCLUSION --EROSION --EXOSTOSES --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --NECROSIS --B-KERATOACANTHOMA 1211010 2300010 0001000 1000000 0001000 2301010 0000010 0100000 ANUS (AN) ............................................ NUMBER EXAMINED: 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --NECROSIS, MUSCULAR 0000010 604 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 23 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- FOOT/FOOTPAD(S) (FP) ................................. NUMBER EXAMINED: 11 7 12 7 0 10 0 NOT REMARKABLE: 1 0 0 0 0 0 0 --EDEMA --EROSION --EXOSTOSES --FIBROSIS --HEMORRHAGE --INFLAMMATION, --INFLAMMATION, --PIGMENT ACUTE CHRONIC ACTIVE 4292020 8 7 12 7 0 7 0 3423040 3 5 10 4 0 6 0 0001000 9 7 11 6 0 9 0 1011010 0000020 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 0 0 1 0 0 0 0 NOT REMARKABLE: 0 0 1 0 0 0 0 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 1 1 1 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --DISLOCATION --EDEMA --EXOSTOSES --FIBROSIS --HEMORRHAGE --INFLAMMATION, --INFLAMMATION, --INFLAMMATION, ACUTE CHRONIC CHRONIC-ACTIVE, SYNOVIAL 1000000 1110000 0111000 0100000 1000000 0110000 0001000 1000000 605 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 24 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 1 1 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC ACTIVE --B-LIPOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0100000 1000000 0100000 0001000 AORTA (AO) ........................................... NUMBER EXAMINED: 3 0 1 0 0 0 0 NOT REMARKABLE: 1 0 0 0 0 0 0 --MINERALIZATION 2010000 PENIS (PE) ........................................... NUMBER EXAMINED: 1 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --EDEMA --HEMORRHAGE --INFLAMMATION, CHRONIC ACTIVE --PROLAPSE 1000000 1000000 1000000 1100000 PREPUTIAL GLAND (PG) ................................. NUMBER EXAMINED: 0 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC 0100000 606 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 25 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 0 1 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --INFLAMMATION, ACUTE --M-CARCINOMA 0000010 0100010 AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 1 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --MINERALIZATION 1000000 LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 1 0 0 2 0 0 0 NOT REMARKABLE: 1 0 0 0 0 0 0 --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001000 0001000 URETER (UE) .......................................... NUMBER EXAMINED: 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --DILATATION --INFLAMMATION, CHRONIC 0000010 0000010 PINNA (PN) ........................................... NUMBER EXAMINED: 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 1 0 607 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 26 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 1 2 1 0 0 0 0 NOT REMARKABLE: 0 1 0 0 0 0 0 --DILATATION, SINUSOIDAL --HYPERPLASIA, LYMPHOCYTIC --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0010000 0110000 1000000 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 SUBCUTANEOUS TIS (SQ) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 TONGUE (TO) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 0 0 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --DILATATION, SINUSOIDAL --INFILTRATE, MACROPHAGE, PIGMENTED 0001000 0001000 608 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 1 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --B-FIBROMA 1000000 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 0 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --DILATED 0100000 609 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 28 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** EYE (EY) ............................................. NUMBER EXAMINED: 65 42 40 65 5 28 0 NOT REMARKABLE: 63 41 40 59 5 26 0 --HEMORRHAGE --HYPERPLASIA, CORNEAL EPITHELIUM --INFLAMMATION, ACUTE --INFLAMMATION, ACUTE, CILIARY --INFLAMMATION, ACUTE, CORNEAL --INFLAMMATION, CHRONIC-ACTIVE, CORNEAL --MINERALIZATION, CORNEAL --ONE EXAMINED --SYNECHIA, ANTERIOR --ULCERATION, CORNEAL --VASCULARIZATION, CORNEAL 0001000 0001000 1001000 0001000 0001000 0000010 0100000 0001000 0000010 2001000 0002020 BRAIN (BR) ........................................... NUMBER EXAMINED: 65 48 48 65 5 34 0 NOT REMARKABLE: 27 10 12 33 5 10 0 --COMPRESSION, VENTRAL --DILATATION, VENTRICULAR --HEMORRHAGE --PIGMENT --VACUOLATION, WHITE MATTER --M-ASTROCYTOMA --I-PITUITARY CARCINOMA 37 37 36 31 0 23 0 25 27 26 19 0 13 0 4774020 0010000 0000010 1000000 0000010 610 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 29 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LIVER (LI) ........................................... NUMBER EXAMINED: 65 55 55 65 5 40 0 NOT REMARKABLE: 35 18 29 17 0 16 0 --CYST --CYST, BILIARY --DEGENERATION, CYSTIC --FIBROSIS --FIBROSIS, CAPSULAR --FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERPLASIA, BILE DUCT --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) --MINERALIZATION --NECROSIS, HEPATOCELLULAR --NECROSIS, INDIVIDUAL HEPATOCYTE --PIGMENT --PIGMENT, HEPATOCELLULAR --PIGMENT, KUPFFER CELL --STASIS, BILE --TELANGIECTASIS --THROMBUS --VACUOLATION, HEPATOCELLULAR 1002000 0100000 0202010 0310010 1000000 0011000 3727020 0221000 1103010 4240130 4 3 6 13 0 1 0 1 0 0 14 5 0 0 9 16 10 19 0 8 0 0100000 0100020 1000000 5446130 0001000 0101000 0000010 0001000 0002000 3614050 0001010 13 14 8 26 0 8 0 611 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 30 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 65 55 55 65 5 40 0 NOT REMARKABLE: 35 18 29 17 0 16 0 --B-ADENOMA, HEPATOCELLULAR --M-CARCINOMA, HEPATOCELLULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0136030 0001000 0000010 LIVER, OIL RED O (LI1) ............................... NUMBER EXAMINED: 5 0 0 5 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --POSITIVE OIL RED O STAINING 5005000 KIDNEY (KD) .......................................... NUMBER EXAMINED: 65 55 55 65 5 40 0 NOT REMARKABLE: 15 7 15 23 4 4 0 --BACTERIAL COLONIES --CAST, PROTEINACEOUS --CYST --DILATATION, PELVIC --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFARCT --INFLAMMATION, CHRONIC --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --NEPHROPATHY, CHRONIC PROGRESSIVE --PYELONEPHRITIS --B-LIPOMA 0000010 14 20 14 18 0 12 0 3004000 5230030 0101000 0100010 0000010 2001000 37 34 28 29 0 27 0 9752140 10 6 3 6 0 6 0 2213030 0000010 612 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 31 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- SPLEEN (SP) .......................................... NUMBER EXAMINED: 65 42 40 65 5 28 0 NOT REMARKABLE: 57 37 33 58 5 21 0 --FIBROSIS, CAPSULAR --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED --PIGMENT, INCREASED --B-HEMANGIOMA 0100000 5226060 3151010 0100000 PANCREAS (PA) ........................................ NUMBER EXAMINED: 65 55 55 65 5 40 0 NOT REMARKABLE: 56 48 48 59 5 35 0 --ATROPHY, ACINAR --HEMORRHAGE --HYPERPLASIA, ACINAR --HYPERPLASIA, ISLET CELLS --PIGMENT --B-ADENOMA, ISLET CELL --I-ENDOMETRIAL STROMAL SARCOMA 5445040 1100000 1000000 1000000 2021000 1411010 0100000 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 65 46 44 65 5 30 0 NOT REMARKABLE: 15 8 7 30 5 3 0 --ANGIECTASIS --ATROPHY, CORTICAL --CYST --DEGENERATION, CYSTIC, CORTICAL --FIBROSIS --HEMATOPOIESIS, EXTRAMEDULLARY 34 32 24 18 0 16 0 0001010 0100000 17 17 19 14 0 14 0 0002000 0000010 613 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 32 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 65 46 44 65 5 30 0 NOT REMARKABLE: 15 8 7 30 5 3 0 --HEMORRHAGE --HYPERPLASIA, CORTICAL --HYPERTROPHY, CORTICAL --MINERALIZATION --NECROSIS, CORTICAL --ONE EXAMINED --PIGMENT --THROMBUS --VACUOLATION --B-ADENOMA --M-CARCINOMA CELL 48 37 34 26 0 20 0 0002000 2020000 1101020 0000010 1000000 7442020 6624040 4236010 2032010 0000010 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 65 45 44 65 5 30 0 NOT REMARKABLE: 64 42 43 61 5 28 0 --ONE EXAMINED --VACUOLATION --B-PHEOCHROMOCYTOMA --M-MALIGNANT PHEOCHROMOCYTOMA 1001010 0001010 0212010 0100000 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 64 41 43 64 5 28 0 NOT REMARKABLE: 57 39 35 60 5 25 0 --DILATATION, SINUSOIDAL --EDEMA 0012000 1010000 614 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 33 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 64 41 43 64 5 28 0 NOT REMARKABLE: 57 39 35 60 5 25 0 --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 2033010 5271020 OVARY (OV) ........................................... NUMBER EXAMINED: 65 47 44 65 5 32 0 NOT REMARKABLE: 52 30 33 37 5 22 0 --CYST --HEMORRHAGE --MINERALIZATION --ONE EXAMINED --PIGMENTED --THROMBUS --B-GRANULOSA/THECA CELL TUMOR 9 13 11 18 0 10 0 1306000 2203000 1102010 0003000 0002000 0001000 TESTIS (TE) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 55 37 35 60 0 27 0 NOT REMARKABLE: 55 37 35 60 0 27 0 615 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 34 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 55 37 35 60 0 27 0 NOT REMARKABLE: 54 37 35 60 0 27 0 --HEMORRHAGE 1000000 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 55 37 35 60 0 28 0 NOT REMARKABLE: 55 37 35 60 0 28 0 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 55 37 35 60 0 28 0 NOT REMARKABLE: 54 37 35 57 0 28 0 --HYPERPLASIA, CARTILAGE 1003000 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 55 37 35 60 0 28 0 NOT REMARKABLE: 55 37 34 60 0 27 0 --HEMORRHAGE 0010010 THYMUS (TH) .......................................... NUMBER EXAMINED: 55 36 35 59 0 25 0 NOT REMARKABLE: 39 27 24 34 0 19 0 --CYST --DEPLETION, LYMPHOCYTIC --FIBROSIS --HEMORRHAGE --M-THYMIC CARCINOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 12 7 10 21 0 5 0 1010000 0001000 3200010 0002000 1001000 616 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 35 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LUNG (LU) ............................................ NUMBER EXAMINED: 55 50 50 60 0 40 0 NOT REMARKABLE: 41 35 36 38 0 19 0 --EDEMA, ALVEOLAR --FIBROSIS, PLEURAL/SUBPLEURAL --FOREIGN BODY --HEMORRHAGE --HYPERPLASIA, TYPE II PNEUMOCYTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --INFLAMMATION, GRANULOMATOUS --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, MACROPHAGE, PIGMENTED --MINERALIZATION --MINERALIZATION, VASCULAR --NECROSIS --THROMBUS --N-ADRENAL CORTICAL CARCINOMA --N-MALIGNANT PHEOCHROMOCYTOMA --N-MAMMARY CARCINOMA 0100010 1000000 0010000 0121040 0200010 2002050 0110000 0010000 8 2 5 12 0 9 0 0011000 0201040 6867080 0000010 0001000 0000010 0100000 0001010 HEART (HT) ........................................... NUMBER EXAMINED: 55 37 35 60 0 28 0 NOT REMARKABLE: 46 32 30 58 0 28 0 --DEGENERATION/NECROSIS --FIBROSIS --MINERALIZATION 9542000 5132000 0011000 617 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 36 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 55 37 35 60 0 28 0 NOT REMARKABLE: 54 37 34 60 0 28 0 --DEGENERATION/NECROSIS 1010000 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 54 37 35 60 0 28 0 NOT REMARKABLE: 54 37 35 60 0 28 0 TRACHEA (TR) ......................................... NUMBER EXAMINED: 55 37 35 60 0 28 0 NOT REMARKABLE: 55 37 35 60 0 28 0 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 55 37 35 60 0 28 0 NOT REMARKABLE: 55 37 35 60 0 28 0 THYROID (TY) ......................................... NUMBER EXAMINED: 55 50 50 60 0 40 0 NOT REMARKABLE: 33 25 34 31 0 25 0 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --HEMORRHAGE --HYPERPLASIA, C-CELL --HYPERPLASIA, FOLLICULAR --INFLAMMATION, CHRONIC --ONE EXAMINED --B-ADENOMA, C-CELL CELL 2203010 13 11 7 13 0 9 0 2612010 1012010 0000010 0000010 0001000 8 13 8 11 0 8 0 618 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 37 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** THYROID (TY) ......................................... NUMBER EXAMINED: 55 50 50 60 0 40 0 NOT REMARKABLE: 33 25 34 31 0 25 0 --B-ADENOMA, FOLLICULAR CELL --M-CARCINOMA, C-CELL 1024010 1000000 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 52 34 34 58 0 27 0 NOT REMARKABLE: 51 34 34 58 0 27 0 --B-ADENOMA 1000000 PITUITARY (PI) ....................................... NUMBER EXAMINED: 55 48 49 59 0 38 0 NOT REMARKABLE: 7 1 1 12 0 2 0 --CYST --HEMORRHAGE --HYPERPLASIA --NECROSIS --PIGMENT --B-ADENOMA --M-CARCINOMA 2456010 33 37 38 30 0 25 0 0000010 0100000 5887040 48 46 48 45 0 34 0 0000010 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 55 37 35 59 0 28 0 NOT REMARKABLE: 44 31 27 51 0 26 0 --HEMORRHAGE --INFLAMMATION, CHRONIC --ONE EXAMINED 1000000 10 6 8 8 0 2 0 1000000 619 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 38 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 55 37 35 59 0 27 0 NOT REMARKABLE: 55 37 35 59 0 27 0 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 55 37 35 60 0 33 0 NOT REMARKABLE: 51 36 32 52 0 32 0 --CYST --EDEMA --EROSION --MINERALIZATION --INFLAMMATION, ACUTE 0000010 3013000 1125000 0011000 0010000 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 55 37 35 60 0 28 0 NOT REMARKABLE: 47 32 32 56 0 23 0 --EDEMA --EROSION --FIBROSIS --HEMORRHAGE --HYPERKERATOSIS, NONGLANDULAR --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION MUCOSA 7534040 2302020 1000000 1002000 1000000 2404020 3000000 2002010 DUODENUM (DU) ........................................ NUMBER EXAMINED: 55 37 35 60 0 28 0 NOT REMARKABLE: 55 37 35 60 0 28 0 620 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 39 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- JEJUNUM (JE) ......................................... NUMBER EXAMINED: 54 38 33 59 0 26 0 NOT REMARKABLE: 53 36 33 59 0 25 0 --CYST, KERATIN --DIVERTICULUM --HYPERPLASIA, GALT (GUT ASSOCIATED LYMPHOID TISSUE) --B-ANGIOMA 0000010 0100000 0100000 1000000 ILEUM (IL) ........................................... NUMBER EXAMINED: 52 37 34 60 0 27 0 NOT REMARKABLE: 52 36 34 60 0 27 0 --HYPERPLASIA, GALT (GUT ASSOCIATED LYMPHOID TISSUE) 0100000 CECUM (CE) ........................................... NUMBER EXAMINED: 54 37 35 60 0 28 0 NOT REMARKABLE: 52 37 35 60 0 28 0 --HEMORRHAGE --PARASITISM 1000000 1000000 COLON (CO) ........................................... NUMBER EXAMINED: 55 37 35 60 0 28 0 NOT REMARKABLE: 54 37 35 59 0 28 0 --EDEMA --PARASITISM 0001000 1000000 621 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 40 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- RECTUM (RE) .......................................... NUMBER EXAMINED: 55 37 35 60 0 27 0 NOT REMARKABLE: 53 35 32 58 0 27 0 --PARASITISM 2232000 SKIN (SK) ............................................ NUMBER EXAMINED: 54 39 36 60 0 28 0 NOT REMARKABLE: 47 34 28 53 0 22 0 --ACUTE INFLAMMATORY EXUDATE --ATROPHY, ADNEXAL --CYST, EPIDERMAL INCLUSION --EDEMA --FAT NECROSIS --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --B-FIBROMA --B-KERATOACANTHOMA --B-LIPOMA --B-PAPILLOMA, SQUAMOUS CELL --M-FIBROSARCOMA --M-SEBACEOUS GLAND CARCINOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0100000 0001010 1000000 0010010 1000000 0001000 1112000 4333020 0011000 1002020 4345050 0010000 0010000 1000000 0000010 0110000 0100000 0000010 622 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 41 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 54 50 50 60 0 40 0 NOT REMARKABLE: 9 7 10 23 0 5 0 --EDEMA --GALACTOCELE --HEMORRHAGE --HYPERPLASIA, GLANDULAR --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --MINERALIZATION --NECROSIS --PIGMENT --B-ADENOMA --B-FIBROADENOMA --M-CARCINOMA --M-FIBROSARCOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0000010 33 32 34 28 0 27 0 5478030 1022000 0143000 0010000 0010010 6886040 2242000 3363010 6463040 23 21 16 13 0 12 0 9 9 15 11 0 6 0 1000000 0000010 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 55 50 49 60 0 40 0 NOT REMARKABLE: 53 50 49 58 0 38 0 --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, CHRONIC --B-LEIOMYOMA --M-CARCINOMA, TRANSITIONAL CELL 0000010 0002020 1000000 1000000 623 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 42 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- CERVIX (CV) .......................................... NUMBER EXAMINED: 55 39 35 60 0 29 0 NOT REMARKABLE: 52 35 33 58 0 24 0 --CYST --DILATATION --HEMORRHAGE --HYPERTROPHY, SMOOTH MUSCLE --INFLAMMATION, ACUTE --NECROSIS --PIGMENT --B-LEIOMYOMA --B-POLYP, ENDOMETRIAL STROMAL --M-LEIOMYOSARCOMA --M-SARCOMA, ENDOMETRIAL STROMAL 1000000 1101010 0100010 0110000 0000010 0100000 1000000 0001010 1201030 0000010 0110000 UTERUS (UT) .......................................... NUMBER EXAMINED: 55 41 39 60 0 32 0 NOT REMARKABLE: 37 27 28 39 0 22 0 --CYST, KERATIN --DILATATION --HEMORRHAGE --HYPERPLASIA, CYSTIC, ENDOMETRIAL --HYPERPLASIA, ENDOMETRIAL --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --NECROSIS --PIGMENT --B-LEIOMYOMA 0001000 15 11 8 12 0 7 0 0012010 3317040 1001000 0011010 2000000 0000010 1001000 1000000 624 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 43 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** UTERUS (UT) .......................................... NUMBER EXAMINED: 55 41 39 60 0 32 0 NOT REMARKABLE: 37 27 28 39 0 22 0 --B-POLYP, ENDOMETRIAL, STROMAL --M-CARCINOMA --M-HEMANGIOSARCOMA --M-LEIOMYOSARCOMA 2223010 0010000 1000000 0001000 VAGINA (VA) .......................................... NUMBER EXAMINED: 55 37 36 58 0 28 0 NOT REMARKABLE: 54 35 34 58 0 27 0 --HEMORRHAGE --B-LEIOMYOMA --B-PAPILLOMA, SQUAMOUS --M-CARCINOMA, SQUAMOUS --I-ENDOMETRIAL STROMAL --I-LEIOMYOSARCOMA CELL CELL SARCOMA 0100000 1000000 0100000 0010000 0010000 0000010 PROSTATE (PR) ........................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 625 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 44 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 CAUSE OF DEATH (CD) .................................. NUMBER EXAMINED: 70 60 60 70 70 40 40 NOT REMARKABLE: 0 0 0 0 0 0 0 --SCHEDULED SACRIFICE --ASTROCYTOMA --C-CELL CARCINOMA --CHRONIC PROGRESSIVE NEPHROPATHY --ENDOMETRIAL STROMAL POLYP --ENDOMETRIAL STROMAL SARCOMA --FIBROMA --FIBROSARCOMA --FRACTURE, CALVARIUM --HEMANGIOSARCOMA --HEPATOCELLULAR ADENOMA --KERATIN CYST --KERATOACANTHOMA --LIPOMA --LIPOSARCOMA --MALIGNANT FIBROUS HISTIOSARCOMA --MALIGNANT LYMPHOMA --MAMMARY CARCINOMA --MAMMARY FIBROADENOMA --PITUITARY ADENOMA --PITUITARY CARCINOMA 33 23 25 39 70 12 40 1000000 1000000 0000010 0000020 0110000 0010000 0110000 0001000 1000000 0010000 0000010 0010000 1000000 0010000 0000010 1000000 0363020 5401010 27 28 22 17 0 18 0 0001010 626 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 45 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** CAUSE OF DEATH (CD) .................................. NUMBER EXAMINED: 70 60 60 70 70 40 40 NOT REMARKABLE: 0 0 0 0 0 0 0 --RENAL MINERALIZATION --SQUAMOUS CELL CARCINOMA --THYMIC CARCINOMA --UNDETERMINED --UTERINE HEMORRHAGE 0001000 0010000 0001000 0005010 0001000 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 9 6 9 9 0 10 0 NOT REMARKABLE: 4 2 3 4 0 3 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED 0010020 4254050 1211020 0100000 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 3 2 5 7 1 4 0 NOT REMARKABLE: 0 0 1 0 0 1 0 --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 2214130 2146020 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 0 4 1 3 0 2 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --FIBROSIS --HEMORRHAGE 0001000 0101000 627 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 46 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 0 4 1 3 0 2 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC --MINERALIZATION --NECROSIS, FAT --PIGMENT --THROMBUS --I-ENDOMETRIAL STROMAL SARCOMA 0001000 0101000 0313020 0001000 0001000 0100000 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 HEMATO NEOPLASIA (HN) ................................ NUMBER EXAMINED: 55 37 35 59 0 28 0 NOT REMARKABLE: 54 37 35 58 0 27 0 --M-LYMPHOMA --M-MALIGNANT FIBROUS HISTIOCYTOMA 1001000 0000010 MESENTERY (MY) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 0 1 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --HEMORRHAGE 0100000 628 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 47 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 0 1 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --INFILTRATE, MACROPHAGE, PIGMENTED 0001000 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 5 6 5 4 0 6 0 NOT REMARKABLE: 3 5 3 4 0 2 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --N-ENDOMETRIAL STROMAL SARCOMA 2000010 1110020 1000020 0010000 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 1 2 0 2 0 2 0 NOT REMARKABLE: 0 1 0 0 0 0 0 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED 1102010 0000010 0001000 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 15 17 18 12 0 6 0 NOT REMARKABLE: 9 12 11 4 0 4 0 --DILATATION, SINUSOIDAL 0111000 629 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 48 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 15 17 18 12 0 6 0 NOT REMARKABLE: 9 12 11 4 0 4 0 --EDEMA --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0010000 4435000 2022010 1113000 0000010 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 0 1 0 1 0 0 0 NOT REMARKABLE: 0 1 0 0 0 0 0 --FRACTURE --HEMORRHAGE 0001000 0001000 TAIL (TI) ............................................ NUMBER EXAMINED: 0 0 1 3 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --CYST, EPIDERMAL INCLUSION --EROSION --INFLAMMATION, ACUTE 0003000 0010010 0013010 ANUS (AN) ............................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 630 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 49 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- FOOT/FOOTPAD(S) (FP) ................................. NUMBER EXAMINED: 7 3 3 1 0 4 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --CYST, EPIDERMAL INCLUSION --EDEMA --EROSION --EXOSTOSES --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC ACTIVE 0100000 2100010 7201030 2100000 5230030 0100000 7320040 0011000 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 4 4 4 2 0 0 0 NOT REMARKABLE: 1 3 3 0 0 0 0 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --N-MAMMARY CARCINOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001000 1110000 1001000 0001000 1000000 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 3 0 1 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --EXOSTOSES --FIBROSIS --INFLAMMATION, CHRONIC 3010000 1010000 2000000 631 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 50 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 1 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --FIBROSIS --HEMORRHAGE --I-TRANSITIONAL CELL CARCINOMA 0100000 0100000 1000000 AORTA (AO) ........................................... NUMBER EXAMINED: 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --MINERALIZATION 0000010 PENIS (PE) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 PREPUTIAL GLAND (PG) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 632 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 51 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 URETER (UE) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 PINNA (PN) ........................................... NUMBER EXAMINED: 0 0 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --HEMORRHAGE 0001000 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 0 0 1 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --DILATATION, SINUSOIDAL 0010000 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 0 0 1 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --M-LIPOSARCOMA 0010000 SUBCUTANEOUS TIS (SQ) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 633 TABLE 45 Incidence of Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 52 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --------------FEMALE------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- TONGUE (TO) .......................................... NUMBER EXAMINED: 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --CYST 0000010 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 1 0 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 634 TABLE 46 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- ** TOP OF LIST ** LIVER (LI) ........................................... NUMBER EXAMINED: 5 5 5 5 --CYST -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 --CYST, BILIARY -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 --DEGENERATION, CYSTIC -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 --FIBROSIS -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 --FIBROSIS, CAPSULAR -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 --FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 635 TABLE 46 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 5 5 5 5 --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 --HEMATOPOIESIS, EXTRAMEDULLARY -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 --HEMORRHAGE -> 5 2 5 4 1> 0 3 0 0 3> 0 0 0 1 TL> 5 5 5 5 MN> 0.0 0.6 0.0 0.6 --HYPERPLASIA, BILE DUCT -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 636 TABLE 46 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 5 5 5 5 --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR -> 5 0 4 0 1> 0 0 1 0 3> 0 5 0 4 4> 0 0 0 1 TL> 5 5 5 5 MN> 0.0 3.0 0.2 3.2 --INFLAMMATION, ACUTE -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 --INFLAMMATION, CHRONIC -> 3 5 5 5 1> 2 0 0 0 TL> 5 5 5 5 MN> 0.4 0.0 0.0 0.0 --INFLAMMATION, CHRONIC-ACTIVE -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 637 TABLE 46 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 5 5 5 5 --MINERALIZATION -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 --NECROSIS, HEPATOCELLULAR -> 5 2 5 4 1> 0 1 0 0 3> 0 2 0 1 TL> 5 5 5 5 MN> 0.0 1.4 0.0 0.6 --NECROSIS, INDIVIDUAL HEPATOCYTE -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 --PIGMENT -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 --PIGMENT, HEPATOCELLULAR -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 --PIGMENT, KUPFFER CELL -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 638 TABLE 46 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 5 5 5 5 --STASIS, BILE -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 --TELANGIECTASIS -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 --THROMBUS -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 --VACUOLATION, HEPATOCELLULAR -> 5 4 5 5 1> 0 1 0 0 TL> 5 5 5 5 MN> 0.0 0.2 0.0 0.0 --B-ADENOMA, HEPATOCELLULAR -> 5 5 5 5 TL> 5 5 5 5 --B-CHOLANGIOMA -> 5 5 5 5 TL> 5 5 5 5 --M-CARCINOMA, HEPATOCELLULAR -> 5 5 5 5 TL> 5 5 5 5 639 TABLE 46 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Week 8 Sacrifice PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 5 5 5 5 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) -> 5 5 5 5 TL> 5 5 5 5 ** END OF LIST ** 640 TABLE 47 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** LIVER (LI) ........................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 --CYST -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --CYST, BILIARY -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --DEGENERATION, CYSTIC -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --FIBROSIS -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --FIBROSIS, CAPSULAR -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 641 TABLE 47 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --HEMATOPOIESIS, EXTRAMEDULLARY -> 4 5 5 5 5 5 5 5 1> 1 0 0 0 0 0 0 0 TL> 5 5 5 5 5 5 5 5 MN> 0.2 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --HEMORRHAGE -> 4 5 5 5 4 5 5 5 1> 1 0 0 0 0 0 0 0 3> 0 0 0 0 1 0 0 0 TL> 5 5 5 5 5 5 5 5 MN> 0.2 0.0 0.0 0.0 0.6 0.0 0.0 0.0 --HYPERPLASIA, BILE DUCT -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 642 TABLE 47 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR -> 5 4 2 0 5 5 5 3 1> 0 1 1 0 0 0 0 0 2> 0 0 1 4 0 0 0 2 3> 0 0 1 1 0 0 0 0 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.2 1.2 2.2 0.0 0.0 0.0 0.8 --INFLAMMATION, ACUTE -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --INFLAMMATION, CHRONIC -> 4 4 2 5 4 1 3 3 1> 0 1 3 0 1 4 2 2 2> 1 0 0 0 0 0 0 0 TL> 5 5 5 5 5 5 5 5 MN> 0.4 0.2 0.6 0.0 0.2 0.8 0.4 0.4 --INFLAMMATION, CHRONIC-ACTIVE -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 643 TABLE 47 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 --MINERALIZATION -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --NECROSIS, HEPATOCELLULAR -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --NECROSIS, INDIVIDUAL HEPATOCYTE -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --PIGMENT -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --PIGMENT, HEPATOCELLULAR -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --PIGMENT, KUPFFER CELL -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 644 TABLE 47 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 --STASIS, BILE -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --TELANGIECTASIS -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --THROMBUS -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --VACUOLATION, HEPATOCELLULAR -> 5 3 3 4 5 5 5 5 1> 0 1 1 1 0 0 0 0 2> 0 1 0 0 0 0 0 0 3> 0 0 1 0 0 0 0 0 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.6 0.8 0.2 0.0 0.0 0.0 0.0 --B-ADENOMA, HEPATOCELLULAR -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 --B-CHOLANGIOMA -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 645 TABLE 47 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Week 14 Sacrifice PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------MALE------- ------FEMALE-----GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 --M-CARCINOMA, HEPATOCELLULAR -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 ** END OF LIST ** 646 TABLE 48 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ** TOP OF LIST ** LIVER (LI) ........................................... NUMBER EXAMINED: 5 10 5 10 --CYST -> 5 10 5 10 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.0 --CYST, BILIARY -> 5 10 5 10 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.0 --DEGENERATION, CYSTIC -> 5 10 5 9 1> 0 0 0 1 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.1 --FIBROSIS -> 5 10 5 10 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.0 --FIBROSIS, CAPSULAR -> 5 10 5 10 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.0 --FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL -> 5 10 5 10 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.0 647 TABLE 48 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 5 10 5 10 --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC -> 5 10 5 9 4> 0 0 0 1 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.4 --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC -> 5 10 5 10 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.0 --HEMATOPOIESIS, EXTRAMEDULLARY -> 5 10 5 10 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.0 --HEMORRHAGE -> 5 10 5 10 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.0 --HYPERPLASIA, BILE DUCT -> 5 10 5 9 1> 0 0 0 1 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.1 648 TABLE 48 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 5 10 5 10 --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR -> 5 0 5 3 2> 0 3 0 3 3> 0 2 0 4 4> 0 5 0 0 TL> 5 10 5 10 MN> 0.0 3.2 0.0 1.8 --INFLAMMATION, ACUTE -> 5 10 5 10 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.0 --INFLAMMATION, CHRONIC -> 2 9 4 9 1> 3 1 1 0 2> 0 0 0 1 TL> 5 10 5 10 MN> 0.6 0.1 0.2 0.2 --INFLAMMATION, CHRONIC-ACTIVE -> 5 10 5 10 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.0 --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) -> 5 10 5 10 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.0 649 TABLE 48 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 5 10 5 10 --MINERALIZATION -> 5 10 5 10 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.0 --NECROSIS, HEPATOCELLULAR -> 5 9 5 8 1> 0 1 0 1 3> 0 0 0 1 TL> 5 10 5 10 MN> 0.0 0.1 0.0 0.4 --NECROSIS, INDIVIDUAL HEPATOCYTE -> 5 10 5 10 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.0 --PIGMENT -> 5 10 5 10 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.0 --PIGMENT, HEPATOCELLULAR -> 5 10 5 10 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.0 --PIGMENT, KUPFFER CELL -> 5 10 5 10 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.0 650 TABLE 48 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 5 10 5 10 --STASIS, BILE -> 5 9 5 8 1> 0 1 0 1 2> 0 0 0 1 TL> 5 10 5 10 MN> 0.0 0.1 0.0 0.3 --TELANGIECTASIS -> 5 10 5 10 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.0 --THROMBUS -> 5 10 5 10 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.0 --VACUOLATION, HEPATOCELLULAR -> 5 1 5 8 1> 0 3 0 2 2> 0 3 0 0 3> 0 3 0 0 TL> 5 10 5 10 MN> 0.0 1.8 0.0 0.2 --B-ADENOMA, HEPATOCELLULAR -> 5 10 +> 0 0 TL> 5 10 59 01 5 10 651 TABLE 48 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Week 53 Sacrifice PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 10 5 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 5 10 5 10 --B-CHOLANGIOMA -> 5 10 5 10 TL> 5 10 5 10 --M-CARCINOMA, HEPATOCELLULAR -> 5 10 TL> 5 10 5 10 5 10 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) ** END OF LIST ** -> 5 10 TL> 5 10 5 10 5 10 652 TABLE 49 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** LIVER (LI) ........................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 --CYST -> 13 22 22 17 10 13 13 15 19 12 3> 0 0 0 0 0 0 0 0 1 0 4> 1 0 0 0 0 0 0 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.3 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.2 0.0 --CYST, BILIARY -> 14 22 22 17 10 13 12 15 20 12 3> 0 0 0 0 0 0 1 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.2 0.0 0.0 0.0 --DEGENERATION, CYSTIC -> 11 17 10 5 9 13 11 15 19 11 1> 2 0 1 5 0 0 1 0 0 0 2> 0 1 2 4 1 0 1 0 1 1 3> 0 3 6 3 0 0 0 0 0 0 4> 1 1 3 0 0 0 0 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.4 0.7 1.6 1.3 0.2 0.0 0.2 0.0 0.1 0.2 --FIBROSIS -> 14 22 21 17 9 13 13 15 20 11 1> 0 0 1 0 1 0 0 0 0 0 2> 0 0 0 0 0 0 0 0 0 1 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.0 0.1 0.0 0.0 0.0 0.0 0.2 653 TABLE 49 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifice PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 --FIBROSIS, CAPSULAR -> 14 22 22 17 10 12 13 15 20 12 3> 0 0 0 0 0 1 0 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.0 0.0 0.2 0.0 0.0 0.0 0.0 --FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL -> 13 21 21 17 10 13 13 14 20 12 2> 0 0 1 0 0 0 0 0 0 0 3> 1 0 0 0 0 0 0 1 0 0 4> 0 1 0 0 0 0 0 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.2 0.2 0.1 0.0 0.0 0.0 0.0 0.2 0.0 0.0 --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC -> 11 12 11 12 9 13 9 13 15 11 1> 0 3 2 0 0 0 0 0 0 0 2> 1 4 2 1 0 0 1 1 1 0 3> 2 3 4 2 1 0 3 0 2 1 4> 0 0 2 2 0 0 0 0 2 0 5> 0 0 1 0 0 0 0 1 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.6 0.9 1.4 0.9 0.3 0.0 0.8 0.5 0.8 0.3 654 TABLE 49 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifice PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC -> 14 22 20 16 10 13 11 14 19 12 1> 0 0 2 0 0 0 1 1 1 0 2> 0 0 0 0 0 0 1 0 0 0 4> 0 0 0 1 0 0 0 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.1 0.2 0.0 0.0 0.2 0.1 0.1 0.0 --HEMATOPOIESIS, EXTRAMEDULLARY -> 14 22 22 17 10 13 13 15 20 12 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --HEMORRHAGE -> 14 20 21 16 8 12 12 14 20 11 1> 0 1 1 0 2 0 1 1 0 0 2> 0 1 0 1 0 0 0 0 0 0 3> 0 0 0 0 0 1 0 0 0 1 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.1 0.0 0.1 0.2 0.2 0.1 0.1 0.0 0.3 --HYPERPLASIA, BILE DUCT -> 10 15 16 13 9 12 12 12 13 11 1> 4 7 6 3 1 1 1 2 6 1 2> 0 0 0 1 0 0 0 1 1 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.3 0.3 0.3 0.3 0.1 0.1 0.1 0.3 0.4 0.1 655 TABLE 49 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifice PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR -> 14 22 22 12 10 13 13 15 15 12 1> 0 0 0 1 0 0 0 0 1 0 2> 0 0 0 2 0 0 0 0 3 0 3> 0 0 0 1 0 0 0 0 1 0 4> 0 0 0 1 0 0 0 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.7 0.0 0.0 0.0 0.0 0.5 0.0 --INFLAMMATION, ACUTE -> 14 22 22 17 9 13 13 15 20 12 1> 0 0 0 0 1 0 0 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.0 0.1 0.0 0.0 0.0 0.0 0.0 --INFLAMMATION, CHRONIC -> 10 9 10 9 7 12 11 13 12 11 1> 4 13 11 8 3 1 2 2 6 1 2> 0 0 1 0 0 0 0 0 2 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.3 0.6 0.6 0.5 0.3 0.1 0.2 0.1 0.5 0.1 --INFLAMMATION, CHRONIC-ACTIVE -> 14 22 22 17 10 13 13 15 20 12 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 656 TABLE 49 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifice PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) -> 14 20 22 16 10 13 12 15 20 10 2> 0 1 0 0 0 0 0 0 0 0 3> 0 0 0 0 0 0 0 0 0 2 4> 0 0 0 1 0 0 0 0 0 0 5> 0 1 0 0 0 0 1 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.3 0.0 0.2 0.0 0.0 0.4 0.0 0.0 0.5 --MINERALIZATION -> 14 22 22 17 10 13 13 15 20 12 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --NECROSIS, HEPATOCELLULAR -> 14 21 20 17 9 12 13 14 20 11 1> 0 0 1 0 0 0 0 1 0 0 2> 0 1 1 0 0 0 0 0 0 0 3> 0 0 0 0 0 0 0 0 0 1 4> 0 0 0 0 1 1 0 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.1 0.1 0.0 0.4 0.3 0.0 0.1 0.0 0.3 --NECROSIS, INDIVIDUAL HEPATOCYTE -> 14 22 22 17 10 13 13 15 20 12 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 657 TABLE 49 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifice PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 --PIGMENT -> 14 21 21 16 9 13 13 15 19 12 1> 0 0 0 0 1 0 0 0 0 0 2> 0 1 1 1 0 0 0 0 1 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.1 0.1 0.1 0.1 0.0 0.0 0.0 0.1 0.0 --PIGMENT, HEPATOCELLULAR -> 14 22 22 17 10 13 13 15 20 12 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --PIGMENT, KUPFFER CELL -> 14 22 22 17 10 13 13 15 20 12 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --STASIS, BILE -> 14 22 22 17 10 13 13 15 20 12 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --TELANGIECTASIS -> 12 16 20 17 10 12 11 15 16 8 1> 1 2 1 0 0 0 1 0 2 3 2> 0 2 1 0 0 0 1 0 1 1 3> 1 2 0 0 0 1 0 0 1 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.3 0.5 0.1 0.0 0.0 0.2 0.2 0.0 0.4 0.4 658 TABLE 49 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifice PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 --THROMBUS -> 14 21 22 17 10 13 13 15 19 12 1> 0 0 0 0 0 0 0 0 1 0 2> 0 1 0 0 0 0 0 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.1 0.0 0.0 0.0 0.0 0.0 0.0 0.1 0.0 --VACUOLATION, HEPATOCELLULAR -> 10 14 13 4 9 9 7 13 7 10 1> 1 3 6 3 1 1 4 1 7 1 2> 2 2 2 5 0 0 2 1 4 1 3> 0 2 1 3 0 3 0 0 1 0 4> 1 1 0 1 0 0 0 0 0 0 5> 0 0 0 1 0 0 0 0 1 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.6 0.8 0.6 1.8 0.1 0.8 0.6 0.2 1.2 0.3 --B-ADENOMA, HEPATOCELLULAR -> 13 20 21 14 10 13 12 14 16 10 +> 1 2 1 3 0 0 1 1 4 2 TL> 14 22 22 17 10 13 13 15 20 12 --B-CHOLANGIOMA -> 14 22 22 17 10 13 13 15 20 12 TL> 14 22 22 17 10 13 13 15 20 12 --M-CARCINOMA, HEPATOCELLULAR -> 14 22 22 17 10 13 13 15 19 12 +> 0 0 0 0 0 0 0 0 1 0 TL> 14 22 22 17 10 13 13 15 20 12 659 TABLE 49 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Terminal and Recovery Sacrifice PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) -> 13 22 22 17 10 13 13 15 20 12 +> 1 0 0 0 0 0 0 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 ** END OF LIST ** 660 TABLE 50 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 36 28 27 33 30 37 37 35 29 28 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** LIVER (LI) ........................................... NUMBER EXAMINED: 36 28 27 33 30 37 37 35 29 28 --CYST -> 36 28 27 31 30 36 37 35 28 28 1> 0 0 0 0 0 0 0 0 1 0 2> 0 0 0 2 0 1 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.1 0.0 0.1 0.0 0.0 0.0 0.0 --CYST, BILIARY -> 36 28 27 33 30 37 37 35 29 28 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --DEGENERATION, CYSTIC -> 33 25 25 27 24 37 37 35 29 28 1> 1 1 0 0 2 0 0 0 0 0 2> 0 1 1 4 3 0 0 0 0 0 3> 2 1 1 1 1 0 0 0 0 0 4> 0 0 0 1 0 0 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.2 0.2 0.2 0.5 0.4 0.0 0.0 0.0 0.0 0.0 --FIBROSIS -> 36 28 27 33 29 37 34 34 29 28 1> 0 0 0 0 0 0 2 1 0 0 3> 0 0 0 0 1 0 1 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.1 0.0 0.1 0.0 0.0 0.0 661 TABLE 50 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 36 28 27 33 30 37 37 35 29 28 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 36 28 27 33 30 37 37 35 29 28 --FIBROSIS, CAPSULAR -> 36 27 27 33 30 37 37 35 29 28 1> 0 1 0 0 0 0 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL -> 36 28 27 33 30 37 37 35 28 28 3> 0 0 0 0 0 0 0 0 1 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.1 0.0 --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC -> 31 26 27 32 29 34 34 35 28 27 1> 2 0 0 0 0 2 0 0 0 0 2> 2 1 0 0 0 0 2 0 0 0 3> 1 0 0 1 1 1 1 0 0 0 5> 0 1 0 0 0 0 0 0 1 1 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.3 0.3 0.0 0.1 0.1 0.1 0.2 0.0 0.2 0.2 --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC -> 35 28 27 33 30 37 37 34 29 28 1> 1 0 0 0 0 0 0 0 0 0 2> 0 0 0 0 0 0 0 1 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.1 0.0 0.0 662 TABLE 50 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 36 28 27 33 30 37 37 35 29 28 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 36 28 27 33 30 37 37 35 29 28 --HEMATOPOIESIS, EXTRAMEDULLARY -> 36 28 27 32 30 36 36 35 26 27 1> 0 0 0 1 0 1 1 0 2 1 2> 0 0 0 0 0 0 0 0 1 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.1 0.0 --HEMORRHAGE -> 34 27 25 33 25 35 36 32 29 26 1> 1 1 0 0 2 1 0 2 0 0 2> 1 0 1 0 0 1 0 1 0 0 3> 0 0 1 0 3 0 1 0 0 2 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.1 0.0 0.2 0.0 0.4 0.1 0.1 0.1 0.0 0.2 --HYPERPLASIA, BILE DUCT -> 31 26 25 26 26 34 35 32 24 28 1> 2 2 0 6 2 3 2 3 5 0 2> 3 0 2 1 1 0 0 0 0 0 3> 0 0 0 0 1 0 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.2 0.1 0.1 0.2 0.2 0.1 0.1 0.1 0.2 0.0 --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR -> 36 28 27 31 28 37 37 35 29 28 2> 0 0 0 0 1 0 0 0 0 0 3> 0 0 0 2 1 0 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.2 0.2 0.0 0.0 0.0 0.0 0.0 663 TABLE 50 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 36 28 27 33 30 37 37 35 29 28 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 36 28 27 33 30 37 37 35 29 28 --INFLAMMATION, ACUTE -> 36 28 27 33 30 37 37 35 29 28 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --INFLAMMATION, CHRONIC -> 28 22 20 22 24 31 27 29 21 21 1> 8 6 7 11 6 5 8 6 8 7 2> 0 0 0 0 0 1 2 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.2 0.2 0.3 0.3 0.2 0.2 0.3 0.2 0.3 0.3 --INFLAMMATION, CHRONIC-ACTIVE -> 36 28 26 33 30 37 36 35 29 28 2> 0 0 0 0 0 0 1 0 0 0 3> 0 0 1 0 0 0 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.1 0.0 0.0 0.0 0.1 0.0 0.0 0.0 --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) -> 36 28 26 32 30 37 37 35 29 28 4> 0 0 0 1 0 0 0 0 0 0 5> 0 0 1 0 0 0 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.2 0.1 0.0 0.0 0.0 0.0 0.0 0.0 --MINERALIZATION -> 36 28 27 33 30 36 37 35 29 28 2> 0 0 0 0 0 1 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.1 0.0 0.0 0.0 0.0 664 TABLE 50 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 36 28 27 33 30 37 37 35 29 28 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 36 28 27 33 30 37 37 35 29 28 --NECROSIS, HEPATOCELLULAR -> 35 26 25 32 27 33 33 32 25 26 1> 0 1 0 0 0 1 1 0 1 0 2> 0 0 1 1 0 1 1 3 1 0 3> 1 1 0 0 3 2 2 0 1 1 4> 0 0 0 0 0 0 0 0 1 1 5> 0 0 1 0 0 0 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.1 0.1 0.3 0.1 0.3 0.2 0.2 0.2 0.3 0.3 --NECROSIS, INDIVIDUAL HEPATOCYTE -> 36 28 27 33 30 37 37 35 28 28 1> 0 0 0 0 0 0 0 0 1 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --PIGMENT -> 36 28 27 33 30 37 36 35 29 28 2> 0 0 0 0 0 0 1 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.1 0.0 0.0 0.0 --PIGMENT, HEPATOCELLULAR -> 36 28 27 33 30 37 37 35 29 27 3> 0 0 0 0 0 0 0 0 0 1 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.1 665 TABLE 50 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 36 28 27 33 30 37 37 35 29 28 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 36 28 27 33 30 37 37 35 29 28 --PIGMENT, KUPFFER CELL -> 36 28 27 33 30 37 37 35 28 28 1> 0 0 0 0 0 0 0 0 1 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --STASIS, BILE -> 36 28 27 33 29 37 37 35 29 28 3> 0 0 0 0 1 0 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.1 0.0 0.0 0.0 0.0 0.0 --TELANGIECTASIS -> 36 27 26 33 30 35 33 34 29 27 1> 0 0 0 0 0 1 2 0 0 1 2> 0 0 0 0 0 0 1 1 0 0 3> 0 1 1 0 0 1 1 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.1 0.1 0.0 0.0 0.1 0.2 0.1 0.0 0.0 --THROMBUS -> 36 28 27 33 30 37 37 35 29 27 3> 0 0 0 0 0 0 0 0 0 1 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.1 666 TABLE 50 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 Unscheduled Deaths PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 36 28 27 33 30 37 37 35 29 28 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 36 28 27 33 30 37 37 35 29 28 --VACUOLATION, HEPATOCELLULAR -> 23 20 16 17 20 28 29 29 18 22 1> 5 3 1 5 3 2 2 3 4 2 2> 2 2 0 2 2 1 0 2 4 1 3> 4 2 6 5 3 2 4 0 3 1 4> 0 0 3 1 1 2 2 1 0 2 5> 2 1 1 3 1 2 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.9 0.6 1.3 1.3 0.8 0.8 0.6 0.3 0.7 0.5 --B-ADENOMA, HEPATOCELLULAR -> 35 26 26 31 30 37 37 33 28 27 +> 1 2 1 2 0 0 0 2 1 1 TL> 36 28 27 33 30 37 37 35 29 28 --B-CHOLANGIOMA -> 36 28 27 32 30 37 37 35 29 28 +> 0 0 0 1 0 0 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 --M-CARCINOMA, HEPATOCELLULAR -> 36 28 27 33 30 37 37 35 29 28 TL> 36 28 27 33 30 37 37 35 29 28 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) ** END OF LIST ** -> 35 27 26 32 29 37 37 35 29 27 +> 1 1 1 1 1 0 0 0 0 1 TL> 36 28 27 33 30 37 37 35 29 28 667 TABLE 51 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN/TISSUE EXAMINED NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** LIVER (LI) ........................................... NUMBER EXAMINED: 65 55 55 65 8 40 1 --CYST -> 64 55 55 63 8 40 1 1> 0 0 0 0 0 0 0 2> 0 0 0 2 0 0 0 3> 0 0 0 0 0 0 0 4> 1 0 0 0 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.1 0.0 0.0 0.1 0.0 0.0 0.0 --CYST, BILIARY -> 65 55 55 65 8 40 1 3> 0 0 0 0 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --DEGENERATION, CYSTIC -> 59 47 41 47 8 33 1 1> 3 1 1 5 0 2 0 2> 0 2 3 8 0 4 0 3> 2 4 7 4 0 1 0 4> 1 1 3 1 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.2 0.4 0.7 0.6 0.0 0.3 0.0 668 TABLE 51 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN/TISSUE EXAMINED NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 65 55 55 65 8 40 1 --FIBROSIS -> 65 55 54 65 8 38 1 1> 0 0 1 0 0 1 0 2> 0 0 0 0 0 0 0 3> 0 0 0 0 0 1 0 TL> 65 55 55 65 8 40 1 MN> 0.0 0.0 0.0 0.0 0.0 0.1 0.0 --FIBROSIS, CAPSULAR -> 65 54 55 65 8 40 1 1> 0 1 0 0 0 0 0 3> 0 0 0 0 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL -> 64 54 54 65 8 40 1 2> 0 0 1 0 0 0 0 3> 1 0 0 0 0 0 0 4> 0 1 0 0 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.0 0.1 0.0 0.0 0.0 0.0 0.0 669 TABLE 51 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN/TISSUE EXAMINED NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 65 55 55 65 8 40 1 --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC -> 57 43 44 59 8 38 1 1> 2 3 2 0 0 0 0 2> 3 5 2 1 0 0 0 3> 3 3 4 3 0 2 0 4> 0 0 2 2 0 0 0 5> 0 1 1 0 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.3 0.5 0.6 0.3 0.0 0.2 0.0 --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC -> 64 55 53 64 8 40 1 1> 1 0 2 0 0 0 0 2> 0 0 0 0 0 0 0 4> 0 0 0 1 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.0 0.0 0.0 0.1 0.0 0.0 0.0 --HEMATOPOIESIS, EXTRAMEDULLARY -> 64 55 55 64 8 40 1 1> 1 0 0 1 0 0 0 2> 0 0 0 0 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 670 TABLE 51 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN/TISSUE EXAMINED NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 65 55 55 65 8 40 1 --HEMORRHAGE -> 62 52 52 64 3 33 1 1> 2 2 1 0 4 4 0 2> 1 1 1 1 0 0 0 3> 0 0 1 0 0 3 0 4> 0 0 0 0 1 0 0 TL> 65 55 55 65 8 40 1 MN> 0.1 0.1 0.1 0.0 1.0 0.3 0.0 --HYPERPLASIA, BILE DUCT -> 56 46 47 54 8 35 1 1> 6 9 6 9 0 3 0 2> 3 0 2 2 0 1 0 3> 0 0 0 0 0 1 0 TL> 65 55 55 65 8 40 1 MN> 0.2 0.2 0.2 0.2 0.0 0.2 0.0 --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR -> 65 54 52 43 1 38 0 1> 0 1 1 1 0 0 0 2> 0 0 1 9 0 1 0 3> 0 0 1 6 5 1 0 4> 0 0 0 6 2 0 1 TL> 65 55 55 65 8 40 1 MN> 0.0 0.0 0.1 0.9 2.9 0.1 4.0 671 TABLE 51 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN/TISSUE EXAMINED NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 65 55 55 65 8 40 1 --INFLAMMATION, ACUTE -> 65 55 55 65 8 39 1 1> 0 0 0 0 0 1 0 TL> 65 55 55 65 8 40 1 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --INFLAMMATION, CHRONIC -> 47 35 32 45 8 31 1 1> 17 20 22 20 0 9 0 2> 1 0 1 0 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.3 0.4 0.4 0.3 0.0 0.2 0.0 --INFLAMMATION, CHRONIC-ACTIVE -> 65 55 54 65 8 40 1 2> 0 0 0 0 0 0 0 3> 0 0 1 0 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.0 0.0 0.1 0.0 0.0 0.0 0.0 --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) -> 65 53 54 63 8 40 1 2> 0 1 0 0 0 0 0 3> 0 0 0 0 0 0 0 4> 0 0 0 2 0 0 0 5> 0 1 1 0 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.0 0.1 0.1 0.1 0.0 0.0 0.0 672 TABLE 51 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN/TISSUE EXAMINED NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 65 55 55 65 8 40 1 --MINERALIZATION -> 65 55 55 65 8 40 1 2> 0 0 0 0 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --NECROSIS, HEPATOCELLULAR -> 64 52 51 63 2 36 0 1> 0 1 1 1 1 0 0 2> 0 1 2 1 1 0 0 3> 1 1 0 0 3 3 1 4> 0 0 0 0 1 1 0 5> 0 0 1 0 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.0 0.1 0.2 0.0 2.0 0.3 3.0 --NECROSIS, INDIVIDUAL HEPATOCYTE -> 65 55 55 65 8 40 1 1> 0 0 0 0 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --PIGMENT -> 65 54 54 64 8 39 1 1> 0 0 0 0 0 1 0 2> 0 1 1 1 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 673 TABLE 51 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN/TISSUE EXAMINED NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 65 55 55 65 8 40 1 --PIGMENT, HEPATOCELLULAR -> 65 55 55 65 8 40 1 3> 0 0 0 0 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --PIGMENT, KUPFFER CELL -> 65 55 55 65 8 40 1 1> 0 0 0 0 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --STASIS, BILE -> 65 55 55 64 8 39 1 1> 0 0 0 1 0 0 0 2> 0 0 0 0 0 0 0 3> 0 0 0 0 0 1 0 TL> 65 55 55 65 8 40 1 MN> 0.0 0.0 0.0 0.0 0.0 0.1 0.0 --TELANGIECTASIS -> 63 48 52 65 8 40 1 1> 1 2 1 0 0 0 0 2> 0 2 1 0 0 0 0 3> 1 3 1 0 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.1 0.3 0.1 0.0 0.0 0.0 0.0 674 TABLE 51 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN/TISSUE EXAMINED NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 65 55 55 65 8 40 1 --THROMBUS -> 65 54 55 65 8 40 1 1> 0 0 0 0 0 0 0 2> 0 1 0 0 0 0 0 3> 0 0 0 0 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --VACUOLATION, HEPATOCELLULAR -> 48 37 32 26 7 29 1 1> 6 7 9 12 1 4 0 2> 4 5 2 10 0 2 0 3> 4 4 8 11 0 3 0 4> 1 1 3 2 0 1 0 5> 2 1 1 4 0 1 0 TL> 65 55 55 65 8 40 1 MN> 0.6 0.7 1.0 1.4 0.1 0.7 0.0 --B-ADENOMA, HEPATOCELLULAR -> 63 51 53 60 8 40 1 +> 2 4 2 5 0 0 0 TL> 65 55 55 65 8 40 1 --B-CHOLANGIOMA -> 65 55 55 64 8 40 1 +> 0 0 0 1 0 0 0 TL> 65 55 55 65 8 40 1 675 TABLE 51 Incidence of Severity of Selected Microscopic Observations Covance 6329-212 3M T-6316.1 All Animals PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ---------------MALE-------------GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN/TISSUE EXAMINED NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 65 55 55 65 8 40 1 --M-CARCINOMA, HEPATOCELLULAR -> 65 55 55 65 8 40 1 +> 0 0 0 0 0 0 0 TL> 65 55 55 65 8 40 1 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) -> 63 54 54 64 8 39 1 +> 2 1 1 1 0 1 0 TL> 65 55 55 65 8 40 1 ** END OF LIST ** 676 APPENDIX 1 Protocol Deviations Covance 6329-212 3M T-6316.1 677 Protocol Deviations Protocol. Test Material. Purity. 98.1% N-EtFOSE (w/w). Covance 6329-212 3M T-6316.1 Actual Procedure. The purity of the test material, as determined by Centre Analytical Laboratories, Inc. on 26 November 2000, was 97.4%. Protocol. Animals. Weight at Initiation of Treatment. Animals will weigh 100 to 300 g at initiation. Actual Procedure. Six animals weighed between 301 to 310 g at initation. Protocol. Husbandry. Water. Water will be provided ad libitum. Actual Procedure. During the a.m. clinical observations on August 18, 1998, it was noted that animals in Cage Rack 3 were without water. Animals appeared in good condition. Animals involved were not specified in the data. During the a.m. clinical observations on June 25, 1998, it was noted that Group 4 males were without water. Animals appeared in good condition. Protocol. Dosing Procedures. Method of Administration. Animals will be offered diet ad libitum, unless otherwise specified. Actual Procedure. Animal No. C91073, Group 6 male, was found without a feed jar on March 8, 1999. Animal No. C90899, Group 3 male, was found without a feed jar on December 8, 1999. Animal No. C91336, Group 4 female, was found with an empty feed jar on May 2, 1999. Animal No. C90934, Group 4 male, was found without a feed jar on January 10, 2000. Protocol. Dosing Procedures. Dose Preparation. All dose preparations will be stored at room temperature. 678 Protocol Deviations (Continued) Covance 6329-212 3M T-6316.1 Actual Procedure. Diets mixed for Weeks 4 through 7 were stored in a refrigerator for approximately 16.5 hours during Week 4. Protocol. Dosing Procedures. Retention Samples. Samples will be collected from each dose preparation. Actual Procedure. Samples for the Week 4 through 7 preparation were not collected until February 23, 1998 (Week 5) from the diet that was inadvertently stored in the refrigerator for approximately 16.5 hours. Protocol. Dosing Procedures. Retention Samples. Unused retention samples will be discarded at least 1 month after completion of the inlife phase. Actual Procedure. Unused retention samples were discarded on March 21, 2000. Group 1 retention samples for Weeks 64 through 67 were not located. Protocol. Dose Analyses. Homogeneity. Samples will be analyzed in triplicate. Actual Procedure. Samples were analyzed in duplicate for the mix on August 26, 1999. Protocol. Observation of Animals. Clinical Observations. Each animal will be observed twice daily (a.m. and p.m.). Actual Procedure. Completion of the p.m. observations on August 10, 1999 was not documented in the data. Protocol. Observation of Animals. Clinical Observations. Animals will be removed from cages weekly and abnormal findings will be recorded. 679 Protocol Deviations (Continued) Covance 6329-212 3M T-6316.1 Actual Procedure. During the weekly data collection, Animal No. C91468, Group 6 female, had severe convulsions and died (April 12, 1999). These observations were not documented. Protocol. Observation of Animals. Body Weights. Body weights will be collected weekly for Weeks 1 through 17. Actual Procedure. Body weight for Week 5 for Animal No. C91010, Group 5 male, were excluded due to an apparent weighing error. Protocol. Observation of Animals. Food Consumption. Food consumption will be measured weekly for Weeks 1 through 16. Actual Procedure. Food consumption for Animal No. C90995, Group 5 male, was excluded for Week 2 due to an apparent weighing error. Protocol. Clinical Pathology. Blood Films. "Blood films will be taken as part of the necropsy procedure." Actual Procedure. A blood film was not taken for Animal No. C91211, Group 2 female, at the Week 105 sacrifice. Protocol. Serum Analyses. Method of Collection. Approximately 3 mL of blood will be collected at the Week 105 sacrifice for possible future serum analyses. Actual Procedure. A sample was not obtained for Animal No. C91211, Group 2 female, at the Week 105 sacrifice. 680 Protocol Deviations (Continued) Covance 6329-212 3M T-6316.1 Protocol. Hepatocellular Proliferation and Biochemical Analyses. Palmitoyl-CoA Oxidase Tissue Collection and Analyses. A 500-mg sample of the right lateral lobe of the liver will be collected. Actual Procedure. A 500-mg sample from the right median lobe was collected for the following animals at Week 14. Animal Nos. C90831, C90797 (Group 2 males), C91281 (Group 3 female), C90780 (Group 1 male), C90960 (Group 4 male), C91241 (Group 2 female), and C91370 (Group 4 female). Protocol. Termination. Uncheduled Sacrifices and Deaths. Animals sacrificed during or after Week 13 will be anesthetized with carbon dioxide. Actual Procedure. Animal Nos. C90806 and C90808 (Group 2 male) and Animal No. C91484 (Group 6 female) were sacrificed using sodium pentobarbital. Protocol. Termination. Unscheduled Sacrifices and Deaths. Animals will be weighed prior to exsanguination. Actual Procedure. The following animals were not weighed: Animal Nos. C91139, Group 1 female; C90806, Group 2 male, C91203, Group 2 female; C91210, Group 2 female; C90849, Group 3 male; C90946, Group 4 male; C90949, Group 4 male; C91358, Group 4 female; C91029, Group 5 male; and C91068, Group 6 male. Protocol. Postmortem Procedures. Organ Weights Specified organs will be weighed at the Week 8 sacrifice. Actual Procedure. Due to an apparent weighing error, organ weights for the left thyroid/parathyroid for Animal Nos. C90756 (Group 1 male), C91017 (Group 5 male), and C91393 (Group 5 female) were excluded. 681 Protocol Deviations (Continued) Covance 6329-212 3M T-6316.1 Protocol. Postmortem Procedures. Tissue Preservation. Lesions will be collected from each animal that dies at an unscheduled interval. Actual Procedure. The last inlife observation for Animal No. C91268, Group 3 female, of crusted footpad for the left hindfoot was confirmed at necropsy. This lesion was not collected and the right hindfoot was inadvertently collected. Protocol. Postmortem Procedures. Tissue Preservation. Mandibular salivary gland will be collected, preserved, and examined microscopically for animals in Group 4. Actual Procedure. Two sets of mandibular salivary glands were noted in the wet tissue collection for Animal No. C91312, Group 4 female. Neither set was submitted for microscopic examination. Protocol. Postmortem Procedures. Tissue Preservation. The following tissues from each animal will be preserved in 10% neutral-bufferd formalin. Actual Procedure. Some tissues, required by protocol, were not available for preservation or histopathologic examination. Missing tissues are listed with appropriate comments in the pathology data sheets for individual animals. Summary tables do not include them as having been examined. These deviations are not expected to have affected the results of the study. 682 Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Author: Peter J. Thomford, PhD Study Completion Date: December 13, 2001 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-212 Sponsor Study Identification: 3M T-6316.1 Volume III of XII Page 683 of 4873 Covance 6329-212 3M T-6316.1 VOLUME III OF XII CONTENTS Page APPENDIX 2.............................................................................................................. 685 Individual Animal Fate Data.................................................................................... 686 Individual Clinical Observations.............................................................................. 711 684 APPENDIX 2 Individual Animal Fate Data Individual Clinical Observations Covance 6329-212 3M T-6316.1 685 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C90711 C90712 C90713 C90714 C90715 C90716 C90717 C90718 C90719 C90720 C90721 C90722 C90723 C90724 C90725 C90726 C90727 C90728 C90729 C90730 C90731 C90732 C90733 C90734 C90735 C90736 C90737 C90738 C90739 C90740 C90741 C90742 C90743 C90744 DOSE GROUP ----- 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- M T M 4 M T 2 2 1 D D T M T 1 T M T D M 3 1 D M T M 2 D M M M T T M TYPE OF DEATH ----------UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH FOURTH INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE SECOND INTERIM SACRIFICE SECOND INTERIM SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE FIRST INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH DATE OF DEATH -------07/26/99 01/26/00 12/10/99 01/27/99 11/05/99 01/25/00 03/20/98 03/20/98 02/18/98 10/05/99 11/26/98 01/25/00 03/22/99 01/25/00 02/18/98 01/26/00 11/29/99 01/25/00 11/01/99 05/20/99 04/29/98 02/18/98 11/22/99 08/02/99 01/25/00 06/13/99 03/20/98 12/15/99 01/09/00 04/26/99 12/22/99 01/25/00 01/26/00 09/21/99 DAY OF STUDY ------547 731 684 367 649 730 54 54 24 618 305 730 421 730 24 731 673 730 645 480 94 24 666 554 730 504 54 689 714 456 696 730 731 604 WEEK OF STUDY ------79 105 98 53 93 105 8 8 4 89 44 105 61 105 4 105 97 105 93 69 14 4 96 80 105 72 8 99 102 66 100 105 105 87 686 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C90745 C90746 C90747 C90748 C90749 C90750 C90751 C90752 C90753 C90754 C90755 C90756 C90757 C90758 C90759 C90760 C90761 C90762 C90763 C90764 C90765 C90766 C90767 C90768 C90769 C90770 C90771 C90772 C90773 C90774 C90775 C90776 C90777 C90778 DOSE GROUP ----- 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- M 3 T 3 M T 4 4 1 4 D 2 M T M D D D M D T M 4 3 2 M M M D M 1 D M T TYPE OF DEATH ----------UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH THIRD INTERIM SACRIFICE TERMINAL SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE FOURTH INTERIM SACRIFICE FOURTH INTERIM SACRIFICE FIRST INTERIM SACRIFICE FOURTH INTERIM SACRIFICE UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH FOURTH INTERIM SACRIFICE THIRD INTERIM SACRIFICE SECOND INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE DATE OF DEATH -------07/12/99 04/29/98 01/25/00 04/29/98 11/03/99 01/26/00 01/27/99 01/27/99 02/18/98 01/27/99 12/21/99 03/20/98 05/28/99 01/26/00 09/14/99 09/10/99 11/18/99 07/27/99 09/21/99 09/04/99 01/25/00 04/10/99 01/27/99 04/29/98 03/20/98 10/27/99 11/11/99 03/29/99 04/25/99 06/09/99 02/18/98 12/22/99 09/29/99 01/27/00 DAY OF STUDY ------533 94 730 94 647 731 367 367 24 367 695 54 488 731 597 593 662 548 604 587 730 440 367 94 54 640 655 428 455 500 24 696 612 732 WEEK OF STUDY ------77 14 105 14 93 105 53 53 4 53 100 8 70 105 86 85 95 79 87 84 105 63 53 14 8 92 94 62 65 72 4 100 88 105 687 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C90779 C90780 C90781 C90782 C90783 C90784 C90785 C90786 C90787 C90788 C90789 C90790 C90791 C90792 C90793 C90794 C90795 C90796 C90797 C90798 C90799 C90800 C90801 C90802 C90803 C90804 C90805 C90806 C90807 C90808 C90809 C90810 C90811 C90812 DOSE GROUP ----- 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- M 3 T T T M T D T T 1 M T M D T M D 3 M T D D M D T M M 3 M T T T T TYPE OF DEATH ----------UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH THIRD INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE DATE OF DEATH -------11/03/99 04/29/98 01/25/00 01/26/00 01/26/00 11/08/99 01/26/00 11/02/99 01/26/00 01/27/00 02/18/98 08/09/99 01/27/00 02/25/99 07/19/99 01/26/00 03/01/99 03/13/99 04/29/98 12/29/99 01/25/00 08/26/99 12/11/99 08/10/99 11/07/99 01/26/00 03/24/99 09/21/98 04/29/98 05/31/99 01/27/00 01/26/00 01/25/00 01/25/00 DAY OF STUDY ------647 94 730 731 731 652 731 646 731 732 24 561 732 396 540 731 400 412 94 703 730 578 685 562 651 731 423 239 94 491 732 731 730 730 WEEK OF STUDY ------93 14 105 105 105 94 105 93 105 105 4 81 105 57 78 105 58 59 14 101 105 83 98 81 93 105 61 35 14 71 105 105 105 105 688 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C90813 C90814 C90815 C90816 C90817 C90818 C90819 C90820 C90821 C90822 C90823 C90824 C90825 C90826 C90827 C90828 C90829 C90830 C90831 C90832 C90833 C90834 C90835 C90836 C90837 C90838 C90839 C90840 C90841 C90842 C90843 C90844 C90845 C90846 DOSE GROUP ----- 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- M 1 T M 1 3 D M D M T M D D M D T T 3 T 1 1 T 3 M T D T M T 3 T M 1 TYPE OF DEATH ----------UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH FIRST INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE THIRD INTERIM SACRIFICE TERMINAL SACRIFICE FIRST INTERIM SACRIFICE FIRST INTERIM SACRIFICE TERMINAL SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE THIRD INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE DATE OF DEATH -------03/18/98 02/18/98 01/25/00 09/15/99 02/18/98 04/29/98 10/28/99 05/25/99 12/21/99 07/12/99 01/25/00 08/09/99 12/11/99 10/29/99 05/11/99 08/08/99 01/26/00 01/26/00 04/29/98 01/26/00 02/18/98 02/18/98 01/27/00 04/29/98 06/21/99 01/26/00 12/19/99 01/25/00 12/03/99 01/26/00 04/29/98 01/26/00 07/26/99 02/18/98 DAY OF STUDY ------52 24 730 598 24 94 641 485 695 533 730 561 685 642 471 560 731 731 94 731 24 24 732 94 512 731 693 730 677 731 94 731 547 24 WEEK OF STUDY ------8 4 105 86 4 14 92 70 100 77 105 81 98 92 68 80 105 105 14 105 4 4 105 14 74 105 99 105 97 105 14 105 79 4 689 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C90847 C90848 C90849 C90850 C90851 C90852 C90853 C90854 C90855 C90856 C90857 C90858 C90859 C90860 C90861 C90862 C90863 C90864 C90865 C90866 C90867 C90868 C90869 C90870 C90871 C90872 C90873 C90874 C90875 C90876 C90877 C90878 C90879 C90880 DOSE GROUP ----- 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- M M M T T 3 1 T T T M T M T M M 3 T T 1 T M T M T D M M D A 3 D M 3 TYPE OF DEATH ----------UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE THIRD INTERIM SACRIFICE FIRST INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE FIRST INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE DATE OF DEATH -------12/13/99 07/05/99 07/19/99 01/26/00 01/25/00 04/29/98 02/18/98 01/26/00 01/27/00 01/25/00 01/14/00 01/25/00 05/19/99 01/25/00 06/04/99 05/17/99 04/29/98 01/26/00 01/27/00 02/18/98 01/26/00 06/17/99 01/25/00 08/02/99 01/25/00 10/24/99 06/17/99 05/03/99 10/19/99 02/15/99 04/29/98 01/29/99 08/09/99 04/29/98 DAY OF STUDY ------687 526 540 731 730 94 24 731 732 730 719 730 479 730 495 477 94 731 732 24 731 508 730 554 730 637 508 463 632 386 94 369 561 94 WEEK OF STUDY ------99 76 78 105 105 14 4 105 105 105 103 105 69 105 71 69 14 105 105 4 105 73 105 80 105 91 73 67 91 56 14 53 81 14 690 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C90881 C90882 C90883 C90884 C90885 C90886 C90887 C90888 C90889 C90890 C90891 C90892 C90893 C90894 C90895 C90896 C90897 C90898 C90899 C90900 C90901 C90902 C90903 C90904 C90905 C90906 C90907 C90908 C90909 C90910 C90911 C90912 C90913 C90914 DOSE GROUP ----- 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 4 4 4 4 4 4 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- T T T T T M M M T 1 1 M T M D M D M M T D 4 D T 3 M 3 T 4 T T M 4 M TYPE OF DEATH ----------SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE FIRST INTERIM SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH FOURTH INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE TERMINAL SACRIFICE FOURTH INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH FOURTH INTERIM SACRIFICE UNSCHEDULED DEATH DATE OF DEATH -------01/26/00 01/26/00 01/27/00 01/26/00 01/26/00 10/18/99 05/17/99 08/09/99 01/25/00 02/18/98 02/18/98 07/12/99 01/26/00 12/10/99 11/13/99 06/07/99 11/17/99 04/01/99 01/17/00 01/26/00 06/21/99 01/27/99 12/29/99 01/25/00 04/29/98 01/08/00 04/29/98 01/25/00 01/27/99 01/27/00 01/26/00 08/16/99 01/27/99 12/22/99 DAY OF STUDY ------731 731 732 731 731 631 477 561 730 24 24 533 731 684 657 498 661 431 722 731 512 367 703 730 94 713 94 730 367 732 731 568 367 696 WEEK OF STUDY ------105 105 105 105 105 91 69 81 105 4 4 77 105 98 94 72 95 62 104 105 74 53 101 105 14 102 14 105 53 105 105 82 53 100 691 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C90915 C90916 C90917 C90918 C90919 C90920 C90921 C90922 C90923 C90924 C90925 C90926 C90927 C90928 C90929 C90930 C90931 C90932 C90933 C90934 C90935 C90936 C90937 C90938 C90939 C90940 C90941 C90942 C90943 C90944 C90945 C90946 C90947 C90948 DOSE GROUP ----- 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- D T 3 4 D M 3 M M 4 M T T T D M M 4 T M 1 M M M 1 M M 1 D T M M T T TYPE OF DEATH ----------UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH TERMINAL SACRIFICE THIRD INTERIM SACRIFICE FOURTH INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH FOURTH INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FOURTH INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE DATE OF DEATH -------11/22/99 01/26/00 04/29/98 01/27/99 07/30/99 01/22/00 04/29/98 04/12/99 09/18/99 01/27/99 11/02/99 01/25/00 01/25/00 01/25/00 05/17/99 10/11/99 12/06/99 01/27/99 01/25/00 01/14/00 02/18/98 12/31/99 01/10/00 05/21/99 02/18/98 04/12/99 09/27/99 02/18/98 10/26/99 01/26/00 11/30/98 07/19/99 01/26/00 01/26/00 DAY OF STUDY ------666 731 94 367 551 727 94 442 601 367 646 730 730 730 477 624 680 367 730 719 24 705 715 481 24 442 610 24 639 731 309 540 731 731 WEEK OF STUDY ------96 105 14 53 79 104 14 64 86 53 93 105 105 105 69 90 98 53 105 103 4 101 103 69 4 64 88 4 92 105 45 78 105 105 692 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C90949 C90950 C90951 C90952 C90953 C90954 C90955 C90956 C90957 C90958 C90959 C90960 C90961 C90962 C90963 C90964 C90965 C90966 C90967 C90968 C90969 C90970 C90971 C90972 C90973 C90974 C90975 C90976 C90977 C90978 C90979 C90980 C90981 C90982 DOSE GROUP ----- 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 5 5 5 5 5 5 5 5 5 5 5 5 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- M 4 4 M T M T T 4 D M 3 1 M M M T 1 4 T D M 2 2 6 6 6 6 6 6 6 6 6 6 TYPE OF DEATH ----------UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH FOURTH INTERIM SACRIFICE FOURTH INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE FOURTH INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE FIRST INTERIM SACRIFICE FOURTH INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH SECOND INTERIM SACRIFICE SECOND INTERIM SACRIFICE SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED DATE OF DEATH -------04/21/98 01/27/99 01/27/99 03/12/99 01/25/00 05/19/99 01/27/00 01/26/00 01/27/99 11/12/99 09/17/99 04/29/98 02/18/98 06/01/99 06/18/99 06/24/99 01/26/00 02/18/98 01/27/99 01/25/00 09/10/99 12/23/99 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 DAY OF STUDY ------86 367 367 411 730 479 732 731 367 656 600 94 24 492 509 515 731 24 367 730 593 697 54 54 54 54 54 54 54 54 54 54 54 54 WEEK OF STUDY ------13 53 53 59 105 69 105 105 53 94 86 14 4 71 73 74 105 4 53 105 85 100 8 8 8 8 8 8 8 8 8 8 8 8 693 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C90983 C90984 C90985 C90986 C90987 C90988 C90989 C90990 C90991 C90992 C90993 C90994 C90995 C90996 C90997 C90998 C90999 C91000 C91001 C91002 C91003 C91004 C91005 C91006 C91007 C91008 C91009 C91010 C91011 C91012 C91013 C91014 C91015 C91016 DOSE GROUP ----- 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- 6 1 6 6 6 2 6 6 6 6 D 6 6 2 6 6 6 6 6 6 D 6 1 6 6 6 6 6 6 6 6 6 6 6 TYPE OF DEATH ----------SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------SACRIFICED AND DISCARDED FIRST INTERIM SACRIFICE SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SECOND INTERIM SACRIFICE SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED UNSCHEDULED DEATH SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SECOND INTERIM SACRIFICE SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED UNSCHEDULED DEATH SACRIFICED AND DISCARDED FIRST INTERIM SACRIFICE SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED DATE OF DEATH -------03/20/98 02/18/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/19/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/11/98 03/20/98 02/18/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 DAY OF STUDY ------54 24 54 54 54 54 54 54 54 54 53 54 54 54 54 54 54 54 54 54 45 54 24 54 54 54 54 54 54 54 54 54 54 54 WEEK OF STUDY ------8 4 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 7 8 4 8 8 8 8 8 8 8 8 8 8 8 694 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C91017 C91018 C91019 C91020 C91021 C91022 C91023 C91024 C91025 C91026 C91027 C91028 C91029 C91030 C91031 C91032 C91033 C91034 C91035 C91036 C91037 C91038 C91039 C91040 C91041 C91042 C91043 C91044 C91045 C91046 C91047 C91048 C91049 C91050 DOSE GROUP ----- 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 6 6 6 6 6 6 6 6 6 6 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- 2 6 6 6 6 6 6 1 6 6 6 6 M 6 6 6 1 6 6 6 1 6 6 6 M D M M M M U M D D TYPE OF DEATH ----------SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------SECOND INTERIM SACRIFICE SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED FIRST INTERIM SACRIFICE SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED UNSCHEDULED DEATH SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED FIRST INTERIM SACRIFICE SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED FIRST INTERIM SACRIFICE SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH DATE OF DEATH -------03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 02/18/98 03/20/98 03/20/98 03/20/98 03/20/98 03/15/98 03/20/98 03/20/98 03/20/98 02/18/98 03/20/98 03/20/98 03/20/98 02/18/98 03/20/98 03/20/98 03/20/98 03/22/99 11/06/99 09/07/99 04/13/99 01/13/99 03/10/98 01/27/00 08/02/99 11/16/98 01/14/00 DAY OF STUDY ------54 54 54 54 54 54 54 24 54 54 54 54 49 54 54 54 24 54 54 54 24 54 54 54 421 650 590 443 353 44 732 554 295 719 WEEK OF STUDY ------8 8 8 8 8 8 8 4 8 8 8 8 7 8 8 8 4 8 8 8 4 8 8 8 61 93 85 64 51 7 105 80 43 103 695 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C91051 C91052 C91053 C91054 C91055 C91056 C91057 C91058 C91059 C91060 C91061 C91062 C91063 C91064 C91065 C91066 C91067 C91068 C91069 C91070 C91071 C91072 C91073 C91074 C91075 C91076 C91077 C91078 C91079 C91080 C91081 C91082 C91083 C91084 DOSE GROUP ----- 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 7 7 7 7 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- M M D U D U D M M U D M M D U M D M D U U U U M M U M D M D 6 6 6 6 TYPE OF DEATH ----------UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST POST-RECOV SAC FIRST POST-RECOV SAC FIRST POST-RECOV SAC FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED DATE OF DEATH -------10/05/99 05/10/99 08/28/99 01/27/00 08/17/99 01/27/00 06/19/99 08/23/99 08/24/99 01/27/00 10/14/99 11/01/99 12/23/99 12/05/99 01/27/00 09/15/99 12/22/99 04/13/98 09/02/99 01/27/00 01/27/00 01/27/00 01/27/00 01/06/00 07/19/99 01/27/00 09/05/99 11/05/99 09/07/99 12/07/99 03/20/98 03/20/98 03/20/98 03/20/98 DAY OF STUDY ------618 470 580 732 569 732 510 575 576 732 627 645 697 679 732 598 696 78 585 732 732 732 732 711 540 732 588 649 590 681 54 54 54 54 WEEK OF STUDY ------89 68 83 105 82 105 73 83 83 105 90 93 100 97 105 86 100 12 84 105 105 105 105 102 78 105 84 93 85 98 8 8 8 8 696 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C91085 C91086 C91087 C91088 C91089 C91090 C91091 C91092 C91093 C91094 C91095 C91096 C91097 C91098 C91099 C91100 C91101 C91102 C91103 C91104 C91105 C91106 C91107 C91108 C91109 C91110 C91111 C91112 C91113 C91114 C91115 C91116 C91117 C91118 DOSE GROUP ----- 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- 6 6 6 6 6 6 6 6 6 6 6 6 6 6 D 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 TYPE OF DEATH ----------SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED UNSCHEDULED DEATH SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED DATE OF DEATH -------03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/05/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 DAY OF STUDY ------54 54 54 54 54 54 54 54 54 54 54 54 54 54 39 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 WEEK OF STUDY ------8 8 8 8 8 8 8 8 8 8 8 8 8 8 6 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 697 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C91119 C91120 C91121 C91122 C91123 C91124 C91125 C91126 C91127 C91128 C91129 C91130 C91131 C91132 C91133 C91134 C91135 C91136 C91137 C91138 C91139 C91140 C91141 C91142 C91143 C91144 C91145 C91146 C91147 C91148 C91149 C91150 C91151 C91152 DOSE GROUP ----- 7 7 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 SEX ------ MALE MALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- 6 6 4 4 D 4 M 2 T M 3 D T M 4 M 1 M M T M 2 D M 1 T 2 M M T M M M 4 TYPE OF DEATH ----------SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED FOURTH INTERIM SACRIFICE FOURTH INTERIM SACRIFICE UNSCHEDULED DEATH FOURTH INTERIM SACRIFICE UNSCHEDULED DEATH SECOND INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH FOURTH INTERIM SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE TERMINAL SACRIFICE SECOND INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FOURTH INTERIM SACRIFICE DATE OF DEATH -------03/20/98 03/20/98 01/27/99 01/27/99 12/09/99 01/27/99 11/27/99 03/20/98 01/25/00 07/02/99 04/29/98 07/13/99 01/25/00 10/05/99 01/27/99 06/28/99 02/18/98 10/11/99 01/10/00 01/27/00 04/02/99 03/20/98 08/06/99 06/14/99 02/18/98 01/26/00 03/20/98 06/28/99 07/14/99 01/25/00 09/27/99 07/05/99 06/21/99 01/27/99 DAY OF STUDY ------54 54 367 367 683 367 671 54 730 523 94 534 730 618 367 519 24 624 715 732 432 54 558 505 24 731 54 519 535 730 610 526 512 367 WEEK OF STUDY ------8 8 53 53 98 53 96 8 105 75 14 77 105 89 53 75 4 90 103 105 62 8 80 73 4 105 8 75 77 105 88 76 74 53 698 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C91153 C91154 C91155 C91156 C91157 C91158 C91159 C91160 C91161 C91162 C91163 C91164 C91165 C91166 C91167 C91168 C91169 C91170 C91171 C91172 C91173 C91174 C91175 C91176 C91177 C91178 C91179 C91180 C91181 C91182 C91183 C91184 C91185 C91186 DOSE GROUP ----- 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- M D 3 M T M T 3 M 2 2 M 1 T D M 1 M 1 T M 3 M T M M M D 3 M T M M M TYPE OF DEATH ----------UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH SECOND INTERIM SACRIFICE SECOND INTERIM SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH DATE OF DEATH -------08/09/99 07/20/99 04/29/98 06/28/99 01/26/00 11/08/99 01/25/00 04/29/98 03/22/99 03/20/98 03/20/98 11/15/99 02/18/98 01/26/00 01/12/00 09/05/99 02/18/98 10/05/99 02/18/98 01/25/00 03/05/99 04/29/98 09/17/99 01/25/00 11/08/99 10/18/99 12/15/98 04/16/99 04/29/98 09/27/99 01/25/00 10/16/99 05/04/99 12/06/99 DAY OF STUDY ------561 541 94 519 731 652 730 94 421 54 54 659 24 731 717 588 24 618 24 730 404 94 600 730 652 631 324 446 94 610 730 629 464 680 WEEK OF STUDY ------81 78 14 75 105 94 105 14 61 8 8 95 4 105 103 84 4 89 4 105 58 14 86 105 94 91 47 64 14 88 105 90 67 98 699 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C91187 C91188 C91189 C91190 C91191 C91192 C91193 C91194 C91195 C91196 C91197 C91198 C91199 C91200 C91201 C91202 C91203 C91204 C91205 C91206 C91207 C91208 C91209 C91210 C91211 C91212 C91213 C91214 C91215 C91216 C91217 C91218 C91219 C91220 DOSE GROUP ----- 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- M T M T D 3 M M M M T 1 M M T M M D 1 M T M D M T D M M D T M M M T TYPE OF DEATH ----------UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE DATE OF DEATH -------06/18/99 01/26/00 11/01/99 01/26/00 12/06/99 04/29/98 10/18/99 01/23/00 12/20/99 08/27/99 01/25/00 02/18/98 11/29/99 09/24/99 01/25/00 09/07/99 07/11/99 03/27/99 02/18/98 06/28/99 01/26/00 06/28/99 06/29/99 07/02/99 01/26/00 05/07/99 07/14/99 12/13/99 02/07/99 01/25/00 05/12/99 11/29/99 09/27/99 01/27/00 DAY OF STUDY ------509 731 645 731 680 94 631 728 694 579 730 24 673 607 730 590 532 426 24 519 731 519 520 523 731 467 535 687 378 730 472 673 610 732 WEEK OF STUDY ------73 105 93 105 98 14 91 104 100 83 105 4 97 87 105 85 76 61 4 75 105 75 75 75 105 67 77 99 54 105 68 97 88 105 700 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C91221 C91222 C91223 C91224 C91225 C91226 C91227 C91228 C91229 C91230 C91231 C91232 C91233 C91234 C91235 C91236 C91237 C91238 C91239 C91240 C91241 C91242 C91243 C91244 C91245 C91246 C91247 C91248 C91249 C91250 C91251 C91252 C91253 C91254 DOSE GROUP ----- 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 3 3 3 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- T 1 3 M M M T M M 1 M T 3 D T T 1 M M M 3 T T M M M M 3 M M 1 T T T TYPE OF DEATH ----------SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------TERMINAL SACRIFICE FIRST INTERIM SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE DATE OF DEATH -------01/25/00 02/18/98 04/29/98 12/02/99 09/02/99 09/13/99 01/25/00 11/08/99 11/08/99 02/18/98 10/26/99 01/26/00 04/29/98 04/29/99 01/26/00 01/25/00 02/18/98 05/10/99 03/01/99 07/29/99 04/29/98 01/25/00 01/26/00 05/07/99 11/24/99 09/21/99 06/17/99 04/29/98 11/29/99 06/14/99 02/18/98 01/25/00 01/25/00 01/26/00 DAY OF STUDY ------730 24 94 676 585 596 730 652 652 24 639 731 94 459 731 730 24 470 400 550 94 730 731 467 668 604 508 94 673 505 24 730 730 731 WEEK OF STUDY ------105 4 14 97 84 86 105 94 94 4 92 105 14 66 105 105 4 68 58 79 14 105 105 67 96 87 73 14 97 73 4 105 105 105 701 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C91255 C91256 C91257 C91258 C91259 C91260 C91261 C91262 C91263 C91264 C91265 C91266 C91267 C91268 C91269 C91270 C91271 C91272 C91273 C91274 C91275 C91276 C91277 C91278 C91279 C91280 C91281 C91282 C91283 C91284 C91285 C91286 C91287 C91288 DOSE GROUP ----- 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- M T M M D M D M M T M M M M M M M 1 M D M M M T T M 3 M M D 1 T 1 3 TYPE OF DEATH ----------UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE TERMINAL SACRIFICE FIRST INTERIM SACRIFICE THIRD INTERIM SACRIFICE DATE OF DEATH -------06/22/99 01/26/00 08/27/99 09/17/99 04/08/99 06/18/99 12/15/99 10/05/99 06/07/99 01/25/00 11/09/99 01/18/00 05/04/99 11/29/99 08/26/99 03/08/99 11/09/99 02/18/98 08/09/99 05/30/99 05/20/99 11/29/99 06/07/99 01/25/00 01/26/00 12/07/99 04/29/98 01/25/99 11/03/99 12/20/99 02/18/98 01/25/00 02/18/98 04/29/98 DAY OF STUDY ------513 731 579 600 438 509 689 618 498 730 653 723 464 673 578 407 653 24 561 490 480 673 498 730 731 681 94 365 647 694 24 730 24 94 WEEK OF STUDY ------74 105 83 86 63 73 99 89 72 105 94 104 67 97 83 59 94 4 81 70 69 97 72 105 105 98 14 53 93 100 4 105 4 14 702 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C91289 C91290 C91291 C91292 C91293 C91294 C91295 C91296 C91297 C91298 C91299 C91300 C91301 C91302 C91303 C91304 C91305 C91306 C91307 C91308 C91309 C91310 C91311 C91312 C91313 C91314 C91315 C91316 C91317 C91318 C91319 C91320 C91321 C91322 DOSE GROUP ----- 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 4 4 4 4 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- M M 1 T 3 T T M D M 3 D T M T 3 M M T M M T D M M M T 1 1 A M M M M TYPE OF DEATH ----------UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE TERMINAL SACRIFICE THIRD INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE FIRST INTERIM SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH DATE OF DEATH -------06/17/99 03/08/99 02/18/98 01/25/00 04/29/98 01/26/00 01/27/00 09/13/99 10/23/99 06/21/99 04/29/98 08/27/99 01/25/00 06/13/99 01/25/00 04/29/98 09/21/99 01/10/00 01/26/00 05/20/99 05/04/99 01/27/00 04/14/99 12/01/99 09/25/99 06/21/99 01/26/00 02/18/98 02/18/98 05/07/98 05/26/99 07/26/99 11/10/99 06/25/98 DAY OF STUDY ------508 407 24 730 94 731 732 596 636 512 94 579 730 504 730 94 604 715 731 480 464 732 444 675 608 512 731 24 24 102 486 547 654 151 WEEK OF STUDY ------73 59 4 105 14 105 105 86 91 74 14 83 105 72 105 14 87 103 105 69 67 105 64 97 87 74 105 4 4 15 70 79 94 22 703 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C91323 C91324 C91325 C91326 C91327 C91328 C91329 C91330 C91331 C91332 C91333 C91334 C91335 C91336 C91337 C91338 C91339 C91340 C91341 C91342 C91343 C91344 C91345 C91346 C91347 C91348 C91349 C91350 C91351 C91352 C91353 C91354 C91355 C91356 DOSE GROUP ----- 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- 4 T M M T M 3 M M T 1 D T T 3 T M T T T 4 M 1 M 4 4 D 1 M 4 4 M 3 T TYPE OF DEATH ----------SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------FOURTH INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE THIRD INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE FOURTH INTERIM SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH FOURTH INTERIM SACRIFICE FOURTH INTERIM SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH FOURTH INTERIM SACRIFICE FOURTH INTERIM SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE TERMINAL SACRIFICE DATE OF DEATH -------01/27/99 01/26/00 01/10/00 09/13/99 01/26/00 04/07/99 04/29/98 09/07/99 07/20/99 01/25/00 02/18/98 12/31/99 01/25/00 01/25/00 04/29/98 01/26/00 12/06/99 01/26/00 01/25/00 01/26/00 01/27/99 01/10/00 02/18/98 06/11/99 01/27/99 01/27/99 03/11/99 02/18/98 11/01/99 01/27/99 01/27/99 09/27/99 04/29/98 01/27/00 DAY OF STUDY ------367 731 715 596 731 437 94 590 541 730 24 705 730 730 94 731 680 731 730 731 367 715 24 502 367 367 410 24 645 367 367 610 94 732 WEEK OF STUDY ------53 105 103 86 105 63 14 85 78 105 4 101 105 105 14 105 98 105 105 105 53 103 4 72 53 53 59 4 93 53 53 88 14 105 704 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 20 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C91357 C91358 C91359 C91360 C91361 C91362 C91363 C91364 C91365 C91366 C91367 C91368 C91369 C91370 C91371 C91372 C91373 C91374 C91375 C91376 C91377 C91378 C91379 C91380 C91381 C91382 C91383 C91384 C91385 C91386 C91387 C91388 C91389 C91390 DOSE GROUP ----- 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 5 5 5 5 5 5 5 5 5 5 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- T M D T T T M D 4 M 4 4 M 3 4 T M M M T D T 3 T 6 6 6 6 6 6 6 6 6 6 TYPE OF DEATH ----------SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH FOURTH INTERIM SACRIFICE UNSCHEDULED DEATH FOURTH INTERIM SACRIFICE FOURTH INTERIM SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE FOURTH INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE THIRD INTERIM SACRIFICE TERMINAL SACRIFICE SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED DATE OF DEATH -------01/25/00 07/02/99 07/25/99 01/26/00 01/26/00 01/27/00 11/29/99 01/02/00 01/27/99 09/17/99 01/27/99 01/27/99 06/21/99 04/29/98 01/27/99 01/26/00 05/17/99 12/06/99 08/27/99 01/25/00 06/06/99 01/27/00 04/29/98 01/25/00 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 DAY OF STUDY ------730 523 546 731 731 732 673 707 367 600 367 367 512 94 367 731 477 680 579 730 497 732 94 730 54 54 54 54 54 54 54 54 54 54 WEEK OF STUDY ------105 75 78 105 105 105 97 101 53 86 53 53 74 14 53 105 69 98 83 105 71 105 14 105 8 8 8 8 8 8 8 8 8 8 705 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 21 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C91391 C91392 C91393 C91394 C91395 C91396 C91397 C91398 C91399 C91400 C91401 C91402 C91403 C91404 C91405 C91406 C91407 C91408 C91409 C91410 C91411 C91412 C91413 C91414 C91415 C91416 C91417 C91418 C91419 C91420 C91421 C91422 C91423 C91424 DOSE GROUP ----- 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- 6 6 2 2 6 6 6 6 6 6 6 6 6 2 6 6 6 6 6 6 6 6 6 6 2 6 6 6 6 6 6 6 1 6 TYPE OF DEATH ----------SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SECOND INTERIM SACRIFICE SECOND INTERIM SACRIFICE SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SECOND INTERIM SACRIFICE SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SECOND INTERIM SACRIFICE SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED FIRST INTERIM SACRIFICE SACRIFICED AND DISCARDED DATE OF DEATH -------03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 02/18/98 03/20/98 DAY OF STUDY ------54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 24 54 WEEK OF STUDY ------8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 4 8 706 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 22 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C91425 C91426 C91427 C91428 C91429 C91430 C91431 C91432 C91433 C91434 C91435 C91436 C91437 C91438 C91439 C91440 C91441 C91442 C91443 C91444 C91445 C91446 C91447 C91448 C91449 C91450 C91451 C91452 C91453 C91454 C91455 C91456 C91457 C91458 DOSE GROUP ----- 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 6 6 6 6 6 6 6 6 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- 6 6 1 6 6 1 6 6 6 6 6 6 6 2 6 6 6 6 1 6 6 6 6 1 6 6 U M U M U M M M TYPE OF DEATH ----------SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED FIRST INTERIM SACRIFICE SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED FIRST INTERIM SACRIFICE SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SECOND INTERIM SACRIFICE SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED FIRST INTERIM SACRIFICE SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED FIRST INTERIM SACRIFICE SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED FIRST POST-RECOV SAC UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH DATE OF DEATH -------03/20/98 03/20/98 02/18/98 03/20/98 03/20/98 02/18/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 02/18/98 03/20/98 03/20/98 03/20/98 03/20/98 02/18/98 03/20/98 03/20/98 01/27/00 06/21/99 01/27/00 08/26/99 01/27/00 07/12/99 11/23/99 04/05/99 DAY OF STUDY ------54 54 24 54 54 24 54 54 54 54 54 54 54 54 54 54 54 54 24 54 54 54 54 24 54 54 732 512 732 578 732 533 667 435 WEEK OF STUDY ------8 8 4 8 8 4 8 8 8 8 8 8 8 8 8 8 8 8 4 8 8 8 8 4 8 8 105 74 105 83 105 77 96 63 707 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 23 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C91459 C91460 C91461 C91462 C91463 C91464 C91465 C91466 C91467 C91468 C91469 C91470 C91471 C91472 C91473 C91474 C91475 C91476 C91477 C91478 C91479 C91480 C91481 C91482 C91483 C91484 C91485 C91486 C91487 C91488 C91489 C91490 C91491 C91492 DOSE GROUP ----- 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 7 7 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- U D M U M M M M U D D M M M U M M M M D U U M M D M U M U U D M 6 6 TYPE OF DEATH ----------SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST POST-RECOV SAC FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH FIRST POST-RECOV SAC FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED DATE OF DEATH -------01/27/00 03/30/99 09/17/99 01/27/00 09/25/99 06/21/99 10/11/99 11/03/99 01/27/00 04/12/99 06/09/99 05/17/99 12/01/99 12/06/99 01/27/00 11/01/99 12/23/99 08/05/99 09/13/99 09/10/99 01/27/00 01/27/00 06/18/99 11/01/99 11/22/99 05/31/99 01/27/00 10/27/99 01/27/00 01/27/00 08/09/99 08/23/99 03/20/98 03/20/98 DAY OF STUDY ------732 429 600 732 608 512 624 647 732 442 500 477 675 680 732 645 697 557 596 593 732 732 509 645 666 491 732 640 732 732 561 575 54 54 WEEK OF STUDY ------105 62 86 105 87 74 90 93 105 64 72 69 97 98 105 93 100 80 86 85 105 105 73 93 96 71 105 92 105 105 81 83 8 8 708 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 24 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C91493 C91494 C91495 C91496 C91497 C91498 C91499 C91500 C91501 C91502 C91503 C91504 C91505 C91506 C91507 C91508 C91509 C91510 C91511 C91512 C91513 C91514 C91515 C91516 C91517 C91518 C91519 C91520 C91521 C91522 C91523 C91524 C91525 C91526 DOSE GROUP ----- 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 TYPE OF DEATH ----------SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED DATE OF DEATH -------03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 DAY OF STUDY ------54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 WEEK OF STUDY ------8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 709 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 PAGE: 25 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C91527 C91528 C91529 C91530 DOSE GROUP ----- 7 7 7 7 SEX -----FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- 6 6 6 6 TYPE OF DEATH ----------SCHEDULED SCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED DATE OF DEATH -------03/20/98 03/20/98 03/20/98 03/20/98 DAY OF STUDY ------54 54 54 54 WEEK OF STUDY ------8 8 8 8 710 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90711 M 79 APPEARANCE HUNCHED THIN 547 526, 533, 540, 547 BEHAVIOR RECUMBENT LATERAL 547 EXCRETION FEW FECES 526, 533, 540, 547 EYES RED DISCHARGE EYE-RIGHT SQUINTED PERI-ORBITAL 414, 449, 456 547 RESPIRATION LABORED 547 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 498, 505, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 421, 428, 512, 519 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 435, 442, 463, 470, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 477, 484, 491 711 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90712 T 105 APPEARANCE HUNCHED MALOCCLUSION THIN 727, 729, 731 92, 99, 106, 113, 120, 127, 176, 183, 190, 197, 204, 211, 218 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708, 715, 722, 727, 729, 731 727, 729, 731 BEHAVIOR HYPOACTIVE 729, 731 EXCRETION FEW FECES NO FECES NONFORMED FECES 729, 731 727 113, 358, 365, 372 SKIN & PELAGE PALE ENTIRE BODY 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 134, 141, 148 155, 162, 169 712 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90713 M 98 APPEARANCE THIN 666, 673, 680, 684 BEHAVIOR RECUMBENT LATERAL 684 EXCRETION NONFORMED FECES 113, 155, 218 EYES PALE EYE-RIGHT 680, 684 RESPIRATION LABORED 684 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA SORE/SCAB AXILLARY REGION-RIGHT TAIL 680, 684 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 684 267, 274, 281 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 120, 127, 225, 232, 337, 344, 428, 435, 22, 29, 36, 43, 50, 134, 141, 148, 162, 239, 246, 253, 260, 351, 358, 365, 372, 442, 449, 456, 463, 57, 64, 71, 78, 85, 169, 176, 183, 190, 288, 295, 302, 309, 379, 386, 393, 400, 470, 477, 484 92, 99, 106 197, 204, 211 316, 323, 330 407, 414, 421 713 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90713 M 98 MASS(ES) AXILLARY REGION-RIGHT WEEK 71 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C 568C, 575C C90714 4 53 EXCRETION NONFORMED FECES 113, 218 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C90715 M 93 APPEARANCE MALOCCLUSION THIN 631, 638, 645, 648-649 648-649 BEHAVIOR HYPOACTIVE 649 EXCRETION FEW FECES 648 NO FECES 649 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA 649 VENTRAL-ABDOMINAL 649 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 714 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90715 M 93 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624 C90716 T 105 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE HEAD-CRANIAL 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687 694, 701, 708, 715, 722, 729-730 526, 533, 540, 547, 554, 561, 568, 575, 582 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 589, 596 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 WART LIKE LESION(S) DORSAL WEEK 105 729-730 715 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90717 2 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 54 C90718 2 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 54 C90719 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C90720 D 89 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617-618 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C90721 D 44 APPEARANCE SWOLLEN LIMB-HIND-LEFT 64, 71, 78, 85 BEHAVIOR RECUMBENT STERNAL 302 EXCRETION FEW FECES NO FECES 302, 305 281 716 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90721 D 44 EYES RED DISCHARGE EYES 281, 288 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA PALE ENTIRE BODY SORE/SCAB LATERAL-CERVICAL-LEFT LATERAL-CERVICAL-RIGHT VENTRAL-CERVICAL 302, 305 302, 305 8, 15, 22, 29 8, 15, 22, 29 281 NORMAL NO REMARKABLE OBSERVATIONS 1, 36, 43, 50, 57, 92, 99, 106, 113, 120, 127, 155, 162, 169 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260 267, 274 MASS(ES) AXILLARY REGION-LEFT WEEK 43 MOUTH WEEK 20 VENTRAL-THORACIC WEEK 42 295C 134, 141, 148 288, 295C, 302C, 305C 717 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90722 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729-730 C90723 M 61 APPEARANCE PROTRUDING PENIS 421 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 C90724 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687 694, 701, 708, 715, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 718 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90724 T 105 NORMAL NO REMARKABLE OBSERVATIONS 568, 575, 582, 589, 596 C90725 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C90726 T 105 APPEARANCE MALOCCLUSION 526, 547, 554, 561, 568 SKIN & PELAGE SORE/SCAB DORSAL PAW-HIND-RIGHT PAWS-HIND YELLOW HAIR COAT 204, 211, 218, 225, 232, 239 701, 708, 715, 722, 729, 731 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330 337, 344 WART LIKE LESION(S) DORSAL WEEK 51 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526 533, 540, 547, 554, 561, 568 719 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90727 M 97 APPEARANCE THIN 666, 673 EYES RED DISCHARGE EYE-RIGHT 673 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 WART LIKE LESION(S) PAW-FRONT-LEFT WEEK 95 PAW-FRONT-RIGHT WEEK 95 659, 666, 673 659, 666, 673 C90728 T 105 EXCRETION FEW FECES 596 SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673 720 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90728 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 680, 687, 694, 701, 708, 715, 722, 729-730 C90729 D 93 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645 C90730 M 69 APPEARANCE HEAD TILT RIGHT HUNCHED THIN 477, 480 477, 480 477, 480 SKIN & PELAGE ROUGH HAIR COAT 477, 480 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 721 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90731 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C90732 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C90733 D 96 APPEARANCE LIMITED USE PAW-HIND-RIGHT PAWS-HIND SWOLLEN PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND 414 526, 533, 540, 547, 554 393, 400 414, 421, 428, 582, 589, 596, 603, 610, 617, 624, 631, 638 526, 533, 540, 547, 554 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT YELLOW HAIR COAT 526, 533, 540, 547, 554 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 645 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 407, 512, 519 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 435, 442, 449, 456, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 463, 470, 477, 484, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 491, 498, 505 WART LIKE LESION(S) EAR-LEFT WEEK 93 645 722 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90733 D 96 WART LIKE LESION(S) TAIL WEEK 82 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666 C90734 M 80 APPEARANCE THIN 547, 554 BEHAVIOR HYPOACTIVE 554 EXCRETION NO FECES 547, 554 EYES RED DISCHARGE EYE-RIGHT 421, 547, 554 SKIN & PELAGE COLD TO TOUCH ENTIRE BODY SORE/SCAB AXILLARY REGION-LEFT 554 491, 498, 505 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 723 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90734 M 80 MASS(ES) AXILLARY REGION-LEFT WEEK 61 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505 512, 519, 526C, 533C, 540C, 547C, 554C C90735 T 105 APPEARANCE SWOLLEN PAW-HIND-LEFT 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722, 729-730 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C90736 M 72 APPEARANCE HUNCHED 502, 504 BEHAVIOR HYPOACTIVE RECUMBENT LATERAL 498, 502, 504 504 EXCRETION FEW FECES 504 724 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90736 M 72 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491 C90737 2 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 54 C90738 D 99 EXCRETION NONFORMED FECES 358 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 393, 400, 484, 491, 575, 582, 666, 673, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 407, 414, 421, 428, 498, 505, 512, 519, 589, 596, 603, 610, 680, 687, 689 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 365, 435, 442, 449, 456, 526, 533, 540, 547, 617, 624, 631, 638, 92, 99, 106 183, 190, 197 274, 281, 288 372, 379, 386 463, 470, 477 554, 561, 568 645, 652, 659 C90739 M 102 APPEARANCE HUNCHED THIN 713-714 713-714 BEHAVIOR HYPOACTIVE 713-714 725 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90739 M 102 EXCRETION NO FECES 713-714 EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL 714 713-714 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C90740 M 66 BEHAVIOR HYPOACTIVE 456 EXCRETION FEW FECES 456 EYES RED DISCHARGE EYES 456 SKIN & PELAGE COLD TO TOUCH ENTIRE BODY 456 726 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90740 M 66 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 351, 414, 421, 428, 435, 442, 449, 456 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 393, 400, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 407 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 358, 365, 92, 99, 106 183, 190, 197 274, 281, 288 372, 379, 386 C90741 M 100 APPEARANCE THIN 680, 687-688, 691-692, 694, 696 BEHAVIOR HYPOACTIVE 696 EXCRETION FEW FECES NONFORMED FECES 680, 687-688, 691-692, 694, 696 575 SKIN & PELAGE SORE/SCAB PAWS-HIND VENTRAL-ABDOMINAL YELLOW HAIR COAT 680, 687-688, 691-692, 694, 696 673 680, 687-688, 691-692, 694, 696 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 727 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90741 M 100 NORMAL NO REMARKABLE OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 WART LIKE LESION(S) VENTRAL-THORACIC-RIGHT WEEK 69 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687-688, 691-692, 694, 696 C90742 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729-730 C90743 T 105 APPEARANCE LIMITED USE LIMB-HIND-RIGHT 701 SKIN & PELAGE SORE/SCAB DORSAL 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 582, 589, 596, 603, 610, 617, 624 728 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90743 T 105 NORMAL NO REMARKABLE OBSERVATIONS 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 708, 715, 722 729, 731 MASS(ES) DORSAL WEEK 49 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414 WART LIKE LESION(S) DORSAL WEEK 61 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 C90744 M 87 APPEARANCE HUNCHED THIN 603-604 603-604 BEHAVIOR HYPOACTIVE 603-604 DISCHARGE NASAL RED IN COLOR 603-604 EXCRETION NO FECES 600, 603-604 EYES SQUINTED PERI-ORBITAL 603-604 729 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 20 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90744 M 87 SKIN & PELAGE RED HAIR COAT LIMBS-FRONT 603-604 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C90745 M 77 APPEARANCE HUNCHED MALOCCLUSION THIN OTHER 533 92, 99, 106, 113, 120, 127, 141, 148, 155, 162, 169, 176, 183 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 295, 302 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505, 512, 519-520, 526, 533 526, 533 520C BEHAVIOR SENSITIVE TO TOUCH 533 EXCRETION FEW FECES 526, 533 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL 526, 533 730 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 21 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90745 M 77 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 477, 484, 491, 498, 505, 512, 519-520, 526, 533 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 134, 267, 274 281, 288 C90746 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C90747 T 105 APPEARANCE HUNCHED THIN 729-730 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 680 687, 694, 701, 708, 715, 722, 729-730 EXCRETION FEW FECES NONFORMED FECES 547, 554, 561 575 EYES RED DISCHARGE EYE-RIGHT 729-730 SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL SORE/SCAB TAIL 526, 533, 540, 547, 554, 561, 568 477, 484 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 731 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 22 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90747 T 105 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 491, 498, 505, 512, 519, 631, 638, 645, 652, 659, 666 MASS(ES) INGUINAL REGION-RIGHT WEEK 97 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729-730C C90748 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C90749 M 93 APPEARANCE PROTRUDING PENIS THIN 645-646 645-647 BEHAVIOR HYPOACTIVE 647 RECUMBENT STERNAL 647 EXCRETION NO FECES 646-647 EYES PALE EYES 647 RED DISCHARGE EYES 647 732 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 23 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90749 M 93 RESPIRATION LABORED 647 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT 647 645-647 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638 C90750 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT PAWS-HIND 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 733 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 24 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90750 T 105 WART LIKE LESION(S) DORSAL WEEK 105 729, 731 C90751 4 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C90752 4 53 EYES CLEAR DISCHARGE EYES RED DISCHARGE EYES 22, 29 15 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113, 120, 127 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309 316, 323, 330, 337, 344, 351, 358, 365, 367 C90753 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 734 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 25 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90754 4 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C90755 D 100 EXCRETION NONFORMED FECES 533 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694-695 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) VENTRAL-CERVICAL WEEK 80 554, 561, 568 735 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 26 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90756 2 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 54 C90757 M 70 APPEARANCE SWOLLEN DORSAL-CERVICAL 484 LIMB-HIND-LEFT 488 BEHAVIOR RECUMBENT LATERAL 488 EXCRETION FEW FECES 477, 484, 488 RESPIRATION AUDIBLE 488 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL 488 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 736 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90758 T 105 APPEARANCE LIMITED USE LIMBS-HIND SWOLLEN LIMBS-HIND PAW-HIND-LEFT 547, 554, 561 547, 554, 561 708, 715, 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 589, 596, 680, 687, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 603, 610, 617, 624, 694, 701 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 631, 638, 645, 652, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 568, 575, 582 659, 666, 673 C90759 M 86 APPEARANCE THIN 554, 561, 568, 575, 582, 589, 596-597 EXCRETION FEW FECES 547, 554, 561, 568, 575, 582, 589, 596-597 SKIN & PELAGE ROUGH HAIR COAT YELLOW HAIR COAT 554, 561, 568, 575, 582, 589, 596-597 561, 568, 575, 582, 589, 596-597 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 737 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 28 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90759 M 86 NORMAL NO REMARKABLE OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 C90760 D 85 APPEARANCE SWOLLEN VENTRAL-CERVICAL 568 BEHAVIOR SENSITIVE TO TOUCH 232, 239, 246, 309 DISCHARGE GENITAL RED IN COLOR 309 EXCRETION DISCOLORED URINE RED IN COLOR 232 ORANGE IN COLOR 246 EYES CLEAR DISCHARGE EYE-LEFT DISCOLORED/LIGHT RED EYE-LEFT RED DISCHARGE EYE-LEFT EYES 533, 540, 547 554, 561, 568, 575, 582, 589, 593 281, 288, 295, 302, 337, 344, 351, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 554 477, 484, 491, 498, 505, 512, 519 738 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 29 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90760 D 85 SKIN & PELAGE SORE/SCAB LIMBS-HIND 337, 344 PAWS-HIND 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526 533, 540, 547, 554, 561, 568, 575, 582, 589, 593 YELLOW HAIR COAT 568, 575, 582, 589, 593 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 253, 260, 267, 274, 316, 323, 330 C90761 D 95 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 662 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C90762 D 79 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT PAWS-HIND 512, 519, 526 533, 540, 547-548 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 739 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 30 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90762 D 79 NORMAL NO REMARKABLE OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505 C90763 M 87 APPEARANCE LIMITED USE LIMBS-FRONT LIMBS-HIND 603-604 603-604 BEHAVIOR ATAXIC 603-604 DISCHARGE ORAL RED IN COLOR 589 EYES RED DISCHARGE EYE-LEFT 596, 603-604 SKIN & PELAGE RED HAIR COAT HEAD-MAXILLARY-LEFT 603-604 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 740 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 31 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90763 M 87 NORMAL NO REMARKABLE OBSERVATIONS 568, 575, 582 C90764 D 84 EXCRETION NONFORMED FECES 575 SKIN & PELAGE SORE/SCAB LATERAL-ABDOMINAL-LEFT 491, 498, 505 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 512, 519, 526, 533, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 540, 547, 554, 561, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 568 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 582, 587 C90765 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 582, 589, 673, 680, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 596, 603, 610, 617, 687, 694, 701, 708, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 624, 631, 638, 645, 715, 722, 729-730 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 652, 659, 666 741 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 32 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90765 T 105 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 82 568, 575 C90766 M 63 BEHAVIOR HYPOACTIVE 440 DISCHARGE ORAL RED IN COLOR 440 NASAL RED IN COLOR 440 EYES RED DISCHARGE EYES 440 RESPIRATION AUDIBLE 440 IRREGULAR 440 LABORED 440 SKIN & PELAGE COLD TO TOUCH ENTIRE BODY ROUGH HAIR COAT SORE/SCAB DORSAL-CERVICAL 440 440 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 99, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260 742 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 33 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90766 M 63 NORMAL NO REMARKABLE OBSERVATIONS 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435 C90767 4 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C90768 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C90769 2 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 54 C90770 M 92 APPEARANCE HUNCHED THIN 629 631, 638, 640 BEHAVIOR HYPOACTIVE 638, 640 EXCRETION FEW FECES NO FECES 629, 631 638, 640 RESPIRATION LABORED 640 743 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 34 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90770 M 92 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL 603, 610, 617, 624 YELLOW HAIR COAT 631, 638, 640 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C90771 M 94 BEHAVIOR HYPOACTIVE RECUMBENT LATERAL 652-653, 655 655 DISCHARGE NASAL RED IN COLOR 652-653, 655 EXCRETION FEW FECES 653, 655 EYES RED DISCHARGE EYES 652-653, 655 744 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 35 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90771 M 94 SKIN & PELAGE SORE/SCAB AXILLARY REGION-RIGHT 589, 596, 603, 610, 617, 624 PAW-HIND-LEFT 603, 610, 617, 624, 631, 638, 645, 652-653, 655 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 582 C90772 M 62 APPEARANCE LIMITED USE LIMBS-HIND 422, 428 EYES RED DISCHARGE EYES 428 SKIN & PELAGE YELLOW HAIR COAT 428 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 745 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 36 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90772 M 62 NORMAL NO REMARKABLE OBSERVATIONS 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421 C90773 D 65 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 455 C90774 M 72 APPEARANCE MALOCCLUSION MISSING TEETH THIN 15, 22, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253 260 141, 148, 155, 162, 169 491, 498, 500 BEHAVIOR HYPOACTIVE 500 EXCRETION FEW FECES 477, 484, 489, 491, 498, 500 RESPIRATION LABORED 500 SKIN & PELAGE RED HAIR COAT HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT 477, 484, 489, 491, 498, 500 477, 484, 489, 491, 498, 500 746 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 37 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90774 M 72 SKIN & PELAGE YELLOW HAIR COAT 477, 484, 489, 491, 498, 500 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113, 120 127, 134, 176, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421 428, 435, 442, 449, 456, 463, 470 C90775 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C90776 D 100 EXCRETION NONFORMED FECES 211, 344 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 218, 302, 309, 400, 407, 491, 498, 582, 589, 673, 680, 22, 29, 36, 43, 50, 127, 134, 141, 148, 225, 232, 239, 246, 316, 323, 330, 337, 414, 421, 428, 435, 505, 512, 519, 526, 596, 603, 610, 617, 687, 694, 696 57, 64, 71, 78, 85, 155, 162, 169, 176, 253, 260, 267, 274, 351, 358, 365, 372, 442, 449, 456, 463, 533, 540, 547, 554, 624, 631, 638, 645, 92, 99, 106 183, 190, 197 281, 288, 295 379, 386, 393 470, 477, 484 561, 568, 575 652, 659, 666 747 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 38 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90777 M 88 APPEARANCE THIN 603, 609-611 BEHAVIOR HYPOACTIVE 603, 609-612 EXCRETION NO FECES 600, 603, 609-612 EYES PALE EYES SQUINTED PERI-ORBITAL 603, 609-612 603, 609-612 HOUSING,FOOD,WATER & MAINT. WATER BOTTLE PLACED ON CAGE 600 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-THORACIC SORE/SCAB PAW-HIND-LEFT 603, 609-612 603, 609-612 540, 547, 554, 561, 568, 575, 582, 589, 596, 600, 603, 609-612 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 748 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 39 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90778 T 105 EYES OPAQUE EYES 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 82 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 722C, 729C, 732C C90779 M 93 APPEARANCE THIN 641, 645, 647 BEHAVIOR HYPOACTIVE 647 EXCRETION FEW FECES NO FECES 641, 645 647 EYES OPAQUE EYE-RIGHT 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 638, 641, 645, 647 749 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 40 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90779 M 93 EYES SQUINTED PERI-ORBITAL 647 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL 647 647 641, 645 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C90780 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 750 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 41 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C90713 M1 491 71 TO 575 83 MASS(ES) AXILLARY REGION-RIGHT WEEK 71 10 X 10MM C90721 M1 295 43 MASS(ES) AXILLARY REGION-LEFT WEEK 43 30 X 30MM 295 43 MASS(ES) VENTRAL-THORACIC WEEK 42 30 X 50MM 302 44 TO 305 44 MASS(ES) VENTRAL-THORACIC WEEK 42 30 X 30MM C90734 M1 526 76 MASS(ES) AXILLARY REGION-LEFT WEEK 61 15 X 15MM 533 77 TO 554 80 MASS(ES) AXILLARY REGION-LEFT WEEK 61 20X20 MM C90745 M1 520 75 APPEARANCE OTHER EXCESSIVE BLEEDING FROM PAWHR, CAUTERIZED WITH SILVER NITRATE C90747 M1 673 97 TO 680 98 MASS(ES) INGUINAL REGION-RIGHT WEEK 97 25 X 50MM 751 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 42 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C90747 M1 687 99 TO 694 100 MASS(ES) INGUINAL REGION-RIGHT WEEK 97 50 X 30MM 701 101 MASS(ES) INGUINAL REGION-RIGHT WEEK 97 60 X 45MM 708 102 TO 722 104 MASS(ES) INGUINAL REGION-RIGHT WEEK 97 70 X 45MM 729 105 TO 730 105 MASS(ES) INGUINAL REGION-RIGHT WEEK 97 80 X 60MM C90778 M1 568 82 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 82 25MM X 20MM 575 83 TO 638 92 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 82 30MM X 25MM 645 93 TO 652 94 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 82 40 X 40MM 659 95 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 82 55 X 50MM 752 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 43 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C90778 M1 666 96 TO 694 100 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 82 60 X 55MM 701 101 TO 722 104 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 82 80 X 75MM 729 105 TO 732 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 82 90 X 80MM 753 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 44 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90781 T 105 SKIN & PELAGE SORE/SCAB TAIL VENTRAL-CERVICAL 477, 484, 547, 554, 561, 568, 575, 603, 610, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 687, 694 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 99, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169, 176 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 456, 463, 470, 491, 498, 505, 512, 519, 526, 533, 540, 582, 589 596, 701, 708, 715, 722, 729-730 WART LIKE LESION(S) VENTRAL-THORACIC-RIGHT WEEK 87 603, 610, 617, 624 C90782 T 105 APPEARANCE SWOLLEN PAW-HIND-LEFT 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT PAWS-HIND 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435 442, 449, 456, 463, 470, 477, 484, 652, 659, 666, 673, 680, 687 694, 701, 708, 715, 722, 729, 731 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 638, 645 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 754 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 45 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90782 T 105 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 491, 498, 505, 512, 519 MASS(ES) DORSAL WEEK 105 729, 731 C90783 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C90784 M 94 APPEARANCE PARALYSIS LIMBS-HIND THIN 651 645, 651-652 BEHAVIOR HYPOACTIVE RECUMBENT LATERAL 651-652 652 755 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 46 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90784 M 94 BEHAVIOR RECUMBENT STERNAL 652 EXCRETION FEW FECES 652 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT VENTRAL-THORACIC 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 638, 645, 651-652 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 651-652 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C90785 T 105 APPEARANCE HUNCHED THIN 728-729, 731 728-729, 731 EXCRETION FEW FECES 728-729, 731 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 728-729, 731 756 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 47 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90785 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610 MASS(ES) AXILLARY REGION-LEFT WEEK 89 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C 694C, 701C, 708C, 715C, 722C, 728-729C, 731C WART LIKE LESION(S) TAIL WEEK 104 728-729, 731 C90786 D 93 EXCRETION NONFORMED FECES 316 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 393, 400, 484, 491, 575, 582, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 589, 596, 603, 610, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 617, 624, 631, 638, 92, 99, 106 183, 190, 197 274, 281, 288 372, 379, 386 463, 470, 477 554, 561, 568 645-646 757 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 48 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90787 T 105 APPEARANCE SWOLLEN PAW-HIND-LEFT 694, 701, 708, 715, 722, 729, 731 SKIN & PELAGE SORE/SCAB SHOULDER-LEFT 92, 99, 106, 113, 120, 127 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 134, 141, 148 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232, 239 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421 428, 435, 442, 449, 456, 463, 470, 631, 638, 645, 652, 659, 666 673, 680, 687 WART LIKE LESION(S) VENTRAL-ABDOMINAL WEEK 69 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624 C90788 T 105 SKIN & PELAGE SORE/SCAB HEAD-CRANIAL LIMB-HIND-LEFT PAWS-HIND SHOULDER-LEFT 15 701 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694 15, 22 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113, 120 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211 758 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 49 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90788 T 105 NORMAL NO REMARKABLE OBSERVATIONS 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 708, 715, 722, 729, 732 C90789 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C90790 M 81 BEHAVIOR HYPOACTIVE 561 RECUMBENT STERNAL 561 EXCRETION FEW FECES 561 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL 561 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554 759 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 50 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90791 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 732 C90792 M 57 APPEARANCE THIN 393, 396 BEHAVIOR HYPOACTIVE 396 EXCRETION NO FECES 396 EYES SQUINTED PERI-ORBITAL 393, 396 RESPIRATION IRREGULAR 396 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 760 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 51 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90793 D 78 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C90794 T 105 APPEARANCE THIN 239, 246 EXCRETION FEW FECES 225, 232, 239 EYES RED DISCHARGE EYE-RIGHT 239 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA VENTRAL-ABDOMINAL RED HAIR COAT HEAD-ENTIRE LIMBS-ALL ROUGH HAIR COAT SORE/SCAB PAW-HIND-RIGHT 225, 232, 239 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 225, 232, 239 239 225, 232, 239 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 761 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 52 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90794 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 414, 421, 428, 435, 442, 449, 456, 463, 470 WART LIKE LESION(S) PAW-HIND-RIGHT WEEK 34 232, 239, 246 C90795 M 58 APPEARANCE THIN 393, 400 DISCHARGE NASAL RED IN COLOR 400 EXCRETION FEW FECES 379, 386, 393, 400 EYES RED DISCHARGE EYES 400 RESPIRATION AUDIBLE 400 SKIN & PELAGE BROWN HAIR COAT GENERALIZED 400 ROUGH HAIR COAT 400 762 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90795 M 58 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372 C90796 D 59 APPEARANCE MALOCCLUSION 99, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183 190, 197, 204, 211, 218, 225, 232, 239 EXCRETION FEW FECES 407, 412 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 344 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 246, 253 260, 267, 274, 281, 288, 295, 302, 309, 316, 386, 393, 400 WART LIKE LESION(S) EAR-LEFT WEEK 47 323, 330, 337, 344, 351, 358, 365, 372, 379 763 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 54 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90797 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C90798 M 101 APPEARANCE MALOCCLUSION 29, 36, 43, 50, 57, 134, 141, 148, 155, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 589, 596, 603, 610, 680, 687, 694, 701, 64, 71, 78, 85, 92, 162, 169, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 617, 624, 631, 638, 703 99, 106, 113, 120, 127 190, 197, 204, 211, 218 281, 288, 295, 302, 309 372, 379, 386, 393, 400 463, 470, 477, 484, 491 554, 561, 568, 575, 582 645, 652, 659, 666, 673 EXCRETION FEW FECES NONFORMED FECES 703 533, 540 RESPIRATION AUDIBLE LABORED 701, 703 701, 703 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C90799 T 105 EXCRETION NONFORMED FECES 708, 715, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 764 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 55 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90799 T 105 NORMAL NO REMARKABLE OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701 WART LIKE LESION(S) MOUTH WEEK 87 603, 610, 617, 624 C90800 D 83 EXCRETION NONFORMED FECES 575, 578 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C90801 D 98 APPEARANCE SWOLLEN LIMB-HIND-RIGHT PAWS-HIND 323, 330, 337 509, 512, 519, 526, 533, 540, 547, 554 EXCRETION NONFORMED FECES 211 765 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 56 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90801 D 98 SKIN & PELAGE SORE/SCAB LIMB-HIND-RIGHT 323 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 218, 302, 309, 414, 421, 505, 561, 645, 652, 22, 29, 36, 43, 50, 127, 134, 141, 148, 225, 232, 239, 246, 316, 344, 351, 358, 428, 435, 442, 449, 568, 575, 582, 589, 659, 666, 673 57, 64, 71, 78, 85, 155, 162, 169, 176, 253, 260, 267, 274, 365, 372, 379, 386, 456, 463, 470, 477, 596, 603, 610, 617, 92, 99, 106 183, 190, 197 281, 288, 295 393, 400, 407 484, 491, 498 624, 631, 638 MASS(ES) LIMB-HIND-RIGHT WEEK 98 680C, 685C WART LIKE LESION(S) HEAD-MAXILLARY-LEFT WEEK 76 526, 533, 540 C90802 M 81 APPEARANCE SWOLLEN PAW-HIND-LEFT THIN 540 554, 561-562 BEHAVIOR RECUMBENT LATERAL 562 EXCRETION FEW FECES 554, 561-562 766 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 57 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90802 M 81 RESPIRATION LABORED 562 SKIN & PELAGE COLD TO TOUCH ENTIRE BODY RED HAIR COAT HEAD-ENTIRE 562 554, 561-562 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 547 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C90803 D 93 EYES OPAQUE EYES 617, 624, 631, 638, 645, 651 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 767 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 58 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90803 D 93 WART LIKE LESION(S) TAIL WEEK 93 645, 651 C90804 T 105 APPEARANCE HUNCHED THIN 729, 731 729, 731 EXCRETION FEW FECES 728-729, 731 EYES OPAQUE EYES 728-729, 731 SKIN & PELAGE SORE/SCAB DORSAL 701, 708, 715, 722, 728-729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 768 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 59 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90805 M 61 APPEARANCE HUNCHED THIN 421, 423 421, 423 BEHAVIOR HYPOACTIVE 423 DISCHARGE GENITAL RED IN COLOR 386, 393 EXCRETION FEW FECES NONFORMED FECES 400, 407, 414, 421, 423 211, 379, 386 EYES SQUINTED PERI-ORBITAL 393, 400, 407, 414, 421, 423 SKIN & PELAGE BROWN HAIR COAT SCROTUM RED HAIR COAT HEAD-ENTIRE 421, 423 407, 414 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372 769 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 60 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90806 M 35 APPEARANCE THIN 239 SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB VENTRAL-ABDOMINAL-LEFT 239 232, 239 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 21 141, 148, 155, 162, 169, 176, 183C, 190C, 197C, 204C, 211C, 218C 225C, 232C, 239C C90807 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C90808 M 71 APPEARANCE HUNCHED THIN 477, 484, 491 463, 470, 477, 484, 491 BEHAVIOR HYPOACTIVE RECUMBENT LATERAL 477, 484, 491 491 EXCRETION FEW FECES 477, 484 770 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 61 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90808 M 71 EXCRETION NO FECES 491 RESPIRATION IRREGULAR LABORED 477, 484 491 SKIN & PELAGE ROUGH HAIR COAT 477, 484, 491 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 C90809 T 105 APPEARANCE SWOLLEN PAW-HIND-LEFT 729, 732 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 771 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 62 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90809 T 105 NORMAL NO REMARKABLE OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 C90810 T 105 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL SORE/SCAB LIMB-HIND-LEFT 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 568, 575, 582, 589 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 708, 715, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 722, 729, 731 C90811 T 105 DISCHARGE GENITAL RED IN COLOR 323 EXCRETION FEW FECES 323, 330, 337 RESPIRATION AUDIBLE 323 LABORED 323 SKIN & PELAGE SORE/SCAB DORSAL 680, 687, 694, 701, 708, 715, 722 772 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 63 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90811 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 344, 351, 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 729-730 WART LIKE LESION(S) DORSAL WEEK 97 673 C90812 T 105 APPEARANCE HUNCHED THIN 729-730 680, 687, 694, 701, 708, 715, 718, 722, 729-730 DISCHARGE NASAL RED IN COLOR 729-730 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA YELLOW HAIR COAT 701, 708, 715, 718, 722, 729-730 687, 694, 701, 708, 715, 718, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 773 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 64 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90812 T 105 NORMAL NO REMARKABLE OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 582, 589, 596 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 WART LIKE LESION(S) VENTRAL-ABDOMINAL-LEFT WEEK 79 547, 554, 561, 568, 575 C90813 M 8 APPEARANCE THIN 52 DISCHARGE ORAL RED IN COLOR 50, 52 EXCRETION FEW FECES 52 EYES RED DISCHARGE EYES 50, 52 RESPIRATION IRREGULAR 52 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL 50, 52 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43 774 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 65 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90814 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C90815 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 WART LIKE LESION(S) DORSAL WEEK 76 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701C 708C, 715C, 722C, 729-730C C90816 M 86 APPEARANCE THIN 582, 589, 596, 598 BEHAVIOR HYPOACTIVE 598 SENSITIVE TO TOUCH 598 EXCRETION FEW FECES 598 SKIN & PELAGE ROUGH HAIR COAT 589, 596, 598 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 775 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 66 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90816 M 86 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575 C90817 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C90818 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C90819 D 92 EXCRETION NONFORMED FECES 491 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 575, 582, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 498, 505, 512, 519, 589, 596, 603, 610, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 526, 533, 540, 547, 617, 624, 631, 638, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 554, 561, 568 641 776 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 67 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90820 M 70 APPEARANCE HUNCHED 485 BEHAVIOR HYPOACTIVE 485 SENSITIVE TO TOUCH 485 EXCRETION FEW FECES 485 EYES RED DISCHARGE EYES 485 RESPIRATION LABORED 485 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 777 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 68 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90821 D 100 EYES DISCOLORED/LIGHT RED EYE-LEFT OPAQUE EYE-LEFT 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 687, 694-695 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 680, 687, 694-695 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 C90822 M 77 APPEARANCE THIN 526, 533 EXCRETION NO FECES 526, 533 SKIN & PELAGE SORE/SCAB TAIL-PROXIMAL 533 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 778 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 69 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90822 M 77 NORMAL NO REMARKABLE OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519 MASS(ES) DORSAL WEEK 76 526C, 533C C90823 T 105 APPEARANCE MALOCCLUSION 680, 687, 694, 701, 708, 715, 722, 729-730 EXCRETION NONFORMED FECES 155, 344 SKIN & PELAGE SORE/SCAB LIMB-HIND-LEFT 631, 638, 645, 652, 659, 666 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 211, 218, 302, 309, 400, 407, 491, 498, 582, 589, 22, 29, 36, 43, 50, 127, 134, 141, 148, 225, 232, 239, 246, 316, 323, 330, 337, 414, 421, 428, 435, 505, 512, 519, 526, 596, 603, 610, 617, 57, 64, 71, 78, 85, 162, 169, 176, 183, 253, 260, 267, 274, 351, 358, 365, 372, 442, 449, 456, 463, 533, 540, 547, 554, 624, 673 92, 99, 106 190, 197, 204 281, 288, 295 379, 386, 393 470, 477, 484 561, 568, 575 779 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 70 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90824 M 81 EXCRETION NO FECES 561 SKIN & PELAGE YELLOW HAIR COAT 561 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554 C90825 D 98 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 685 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C90826 D 92 EXCRETION NONFORMED FECES 491, 575 SKIN & PELAGE SORE/SCAB TAIL 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 463, 470, 477, 484 780 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 71 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90826 D 92 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 498, 505 512, 519, 526, 533, 540, 547, 554, 561, 568, 582, 589, 596, 603 610, 617, 624, 631, 638, 642 C90827 M 68 BEHAVIOR HYPOACTIVE 471 SENSITIVE TO TOUCH 471 DISCHARGE ORAL CLEAR 471 RESPIRATION LABORED 471 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 781 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 72 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90828 D 80 EYES DILATED PUPIL EYES 547, 554, 560 SKIN & PELAGE SORE/SCAB DORSAL 491, 498, 505, 512, 519, 526, 533, 540 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316 WART LIKE LESION(S) DORSAL WEEK 47 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 C90829 T 105 APPEARANCE OBESE 701, 708, 715, 722, 729 EXCRETION NONFORMED FECES 344 SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL SORE/SCAB AXILLARY REGION-RIGHT 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722, 729, 731 782 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 73 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90829 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519 MASS(ES) AXILLARY REGION-RIGHT WEEK 91 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C 708C, 715C, 722C, 729C, 731C WART LIKE LESION(S) VENTRAL-THORACIC-RIGHT WEEK 84 582, 589, 596, 603, 610, 617, 624 C90830 T 105 APPEARANCE PROTRUDING PENIS 680, 687, 694 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 701, 708, 715, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 722, 729, 731 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 783 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 74 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90831 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C90832 T 105 APPEARANCE MALOCCLUSION MISSING TEETH SWOLLEN NOSE 225, 379, 547, 631, 722, 232, 386, 554, 638, 729, 239, 393, 561, 645, 731 246, 400, 568, 652, 295, 407, 575, 659, 323, 414, 582, 666, 330, 498, 589, 673, 337, 505, 596, 680, 344, 512, 603, 687, 351, 358, 365, 372 519, 526, 533, 540 609-610, 617, 624 694, 701, 708, 715 680, 687, 694, 701, 708, 715, 722, 729, 731 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 DISCHARGE NASAL RED IN COLOR 505 EXCRETION FEW FECES 505 EYES PALE EYES 505 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 253, 260, 267, 274, 281, 288, 302, 309, 316, 421 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 784 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 75 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90833 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C90834 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C90835 T 105 APPEARANCE MALOCCLUSION 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547, 554, 561, 568, 575, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 547 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 582 MASS(ES) VENTRAL-ABDOMINAL WEEK 77 533C, 540C WART LIKE LESION(S) VENTRAL-ABDOMINAL WEEK 85 589, 596, 603, 610, 617, 624 785 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 76 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90836 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C90837 M 74 APPEARANCE THIN 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 BEHAVIOR SENSITIVE TO TOUCH 512 EYES RED DISCHARGE EYE-RIGHT 512 SKIN & PELAGE SORE/SCAB HEAD-MAXILLARY-RIGHT 379, 512 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337 344, 386, 393, 400, 407, 414 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 51 WEEK 61 351, 358, 365, 372, 379 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C 498C, 505C, 512C WART LIKE LESION(S) TAIL WEEK 19 127, 134, 141, 148, 155, 162, 169 786 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 77 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90838 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 211, 218, 302, 309, 393, 400, 484, 491, 575, 582, 22, 29, 36, 43, 50, 127, 134, 141, 155, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 589, 596, 603, 610, 57, 64, 71, 78, 85, 162, 169, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 617, 624 92, 99, 106 190, 197, 204 281, 288, 295 372, 379, 386 463, 470, 477 554, 561, 568 WART LIKE LESION(S) MOUTH WEEK 91 TAIL WEEK 22 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722, 729, 731 148 C90839 D 99 APPEARANCE SWOLLEN LIMB-HIND-LEFT 546-547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 631, 638, 645, 652, 659, 666, 673, 680, 687, 693 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL BLUE SKIN PAW-HIND-LEFT SORE/SCAB VENTRAL-CERVICAL 680, 687, 693 547, 554 680, 687, 693 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 787 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 78 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90839 D 99 NORMAL NO REMARKABLE OBSERVATIONS 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 C90840 T 105 APPEARANCE SWOLLEN PAWS-HIND 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA SORE/SCAB LATERAL-ABDOMINAL-RIGHT PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND 680, 687, 694, 701, 708, 715, 722, 729-730 344, 351, 358, 365, 372 330, 337, 344, 351, 358 274, 281, 288 295, 302, 309, 316, 323, 365, 372, 379, 386, 393, 400, 407, 414 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687 694, 701, 708, 715, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 351C, 358C, 365C, 372C, 379C 788 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 79 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C90785 M2 617 89 TO 624 90 MASS(ES) AXILLARY REGION-LEFT WEEK 89 10 X 10MM 631 91 TO 638 92 MASS(ES) AXILLARY REGION-LEFT WEEK 89 20 X 20MM 645 93 TO 652 94 MASS(ES) AXILLARY REGION-LEFT WEEK 89 35 X 35MM 659 95 TO 694 100 MASS(ES) AXILLARY REGION-LEFT WEEK 89 40 X 40MM 701 101 MASS(ES) AXILLARY REGION-LEFT WEEK 89 50 X 50MM 708 102 TO 731 105 MASS(ES) AXILLARY REGION-LEFT WEEK 89 55 X 65MM C90801 M2 680 98 TO 685 98 MASS(ES) LIMB-HIND-RIGHT WEEK 98 40 X 40MM C90806 M2 183 27 TO 190 28 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 21 10 X 10MM 789 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 80 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C90806 M2 197 29 TO 204 30 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 21 30 X 15MM 211 31 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 21 70 X 50MM 218 32 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 21 80 X 65MM 225 33 TO 232 34 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 21 85 X 70MM 239 35 TO 239 35 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 21 95 X 100MM 239 35 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 21 95 X 100MM C90815 M2 701 101 TO 730 105 WART LIKE LESION(S) DORSAL WEEK 76 15 X 15MM C90822 M2 526 76 TO 533 77 MASS(ES) DORSAL WEEK 76 10 X 10MM 790 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 81 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C90829 M2 631 91 TO 645 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 91 20 X 20MM 652 94 TO 659 95 MASS(ES) AXILLARY REGION-RIGHT WEEK 91 25 X 25MM 666 96 MASS(ES) AXILLARY REGION-RIGHT WEEK 91 30 X 25MM 673 97 MASS(ES) AXILLARY REGION-RIGHT WEEK 91 35 X 30MM 680 98 TO 701 101 MASS(ES) AXILLARY REGION-RIGHT WEEK 91 15 X 15MM 708 102 TO 731 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 91 20 X 15MM C90835 M2 533 77 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL WEEK 77 10 X 10MM C90837 M2 421 61 TO 505 73 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 61 20 X 20MM 791 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 82 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C90837 M2 512 74 TO 512 74 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 61 20 X 25MM 512 74 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 61 20 X 20MM C90840 M2 351 51 TO 379 55 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 10 X 10MM 792 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 83 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90841 M 97 APPEARANCE HUNCHED 677 THIN 677 EXCRETION FEW FECES 673, 677 EYES DISCOLORED/LIGHT RED EYES 677 RESPIRATION LABORED 677 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND VENTRAL-ABDOMINAL YELLOW HAIR COAT 659, 666, 673, 677 309, 316, 323, 330, 337, 344, 351 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 582, 589, 596, 603, 610 673, 677 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 435, 442, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 358, 365, 372, 379, 449, 456, 463, 470 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 386, 393, 400, 407, 92, 99, 106 183, 190, 197 274, 281, 288 414, 421, 428 793 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 84 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90841 M 97 MASS(ES) VENTRAL-ABDOMINAL WEEK 83 575C C90842 T 105 EXCRETION FEW FECES 680, 687, 694 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 701, 708, 715, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 722, 729, 731 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C90843 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C90844 T 105 APPEARANCE MALOCCLUSION SWOLLEN PAW-HIND-RIGHT 708 725, 729, 731 SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL SCALY SKIN TAIL SORE/SCAB TAIL 526 526 568, 575 794 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 85 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90844 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 533, 540, 547, 554, 561, 582, 589 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 715, 722 WART LIKE LESION(S) TAIL WEEK 22 148 C90845 M 79 APPEARANCE THIN 547 EXCRETION NO FECES 547 SKIN & PELAGE BROWN HAIR COAT GENERALIZED 547 BROWN SKIN PAWS-ALL 547 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 795 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 86 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90845 M 79 NORMAL NO REMARKABLE OBSERVATIONS 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 C90846 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C90847 M 99 APPEARANCE THIN 680, 687 BEHAVIOR HYPOACTIVE 687 EXCRETION NO FECES 673, 680, 687 EYES RED DISCHARGE EYES 687 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT PAWS-HIND YELLOW HAIR COAT 526, 533, 540, 547, 554, 561, 568 575, 582, 589, 596, 603, 608, 610, 617, 624, 631, 638, 645, 652 659, 666, 673 687 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 796 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 87 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90847 M 99 NORMAL NO REMARKABLE OBSERVATIONS 477, 484, 491, 498, 505, 512, 519 C90848 M 76 APPEARANCE MALOCCLUSION 526 DISCHARGE NASAL RED IN COLOR 526 EYES RED DISCHARGE EYES 526 RESPIRATION AUDIBLE 526 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 797 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 88 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90849 M 78 APPEARANCE THIN 520, 526, 533, 540 BEHAVIOR SENSITIVE TO TOUCH 512, 519-520, 526, 533, 540 EXCRETION NONFORMED FECES 520, 526, 533, 540 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 491, 498, 505, 512, 519-520, 526, 533, 540 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C90850 T 105 APPEARANCE MALOCCLUSION 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729, 731 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 680, 687, 694, 701, 708, 715, 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 798 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 89 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90850 T 105 NORMAL NO REMARKABLE OBSERVATIONS 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 C90851 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT PAWS-HIND 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C90852 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C90853 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 799 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 90 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90854 T 105 EXCRETION FEW FECES 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C90855 T 105 APPEARANCE PROTRUDING PENIS 680 EXCRETION NONFORMED FECES 281, 708, 715 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 302, 309, 393, 400, 484, 491, 575, 582, 666, 673, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 589, 596, 603, 610, 687, 694, 701, 722 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 617, 624, 631, 638, 92, 99, 106 183, 190, 197 274, 288, 295 372, 379, 386 463, 470, 477 554, 561, 568 645, 652, 659 WART LIKE LESION(S) DORSAL-CERVICAL-LEFT WEEK 105 729, 732 800 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 91 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90856 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729-730 C90857 M 103 APPEARANCE HUNCHED THIN 718-719 680, 687, 694, 701, 708, 715, 718-719 BEHAVIOR HYPOACTIVE 719 EXCRETION FEW FECES 715, 718-719 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 715, 718-719 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 801 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 92 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90857 M 103 NORMAL NO REMARKABLE OBSERVATIONS 659, 666, 673 C90858 T 105 DISCHARGE NASAL RED IN COLOR 589, 596 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708, 715, 722 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 729-730 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C90859 M 69 APPEARANCE THIN 463, 470, 477, 479 BEHAVIOR HYPOACTIVE 479 RESPIRATION AUDIBLE 479 SKIN & PELAGE YELLOW HAIR COAT 463, 470, 477, 479 802 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 93 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90859 M 69 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 C90860 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729-730 C90861 M 71 BEHAVIOR HYPOACTIVE 495 RECUMBENT STERNAL 495 SENSITIVE TO TOUCH 495 EXCRETION FEW FECES NO FECES 477, 484, 491 495 SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL VENTRAL-ABDOMINAL ROUGH HAIR COAT 477, 484 495 477, 484, 491, 495 803 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 94 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90861 M 71 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 281, 288, 295 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 316, 323, 407, 414, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 330, 337, 344, 351, 421, 428, 435, 442, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 358, 365, 372, 379, 449, 456, 463, 470 92, 99, 106 183, 190, 197 274, 302, 309 386, 393, 400 C90862 M 69 APPEARANCE HUNCHED 477 THIN 477 BEHAVIOR ATAXIC 477 EYES SQUINTED PERI-ORBITAL 477 RESPIRATION IRREGULAR 477 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 804 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 95 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90863 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C90864 T 105 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB SHOULDER-LEFT 680, 687, 694, 701, 708, 715, 722, 729, 731 365, 372, 379 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 421, 428, 512, 519, 603, 610, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 435, 442, 449, 456, 526, 533, 540, 547, 617, 624, 631, 638, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 267, 386, 393, 463, 470, 477, 484, 554, 561, 568, 575, 645, 652, 659, 666, 92, 99, 106 183, 190, 197 400, 407, 414 491, 498, 505 582, 589, 596 673 MASS(ES) DORSAL WEEK 98 SHOULDER-LEFT WEEK 40 680, 687, 694, 701, 708, 715, 722, 729, 731 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 WART LIKE LESION(S) SHOULDER-LEFT WEEK 37 253, 260 805 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 96 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90865 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 715, 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C90866 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C90867 T 105 EXCRETION FEW FECES 645, 652, 659 EYES RED DISCHARGE EYE-LEFT 680, 687, 694, 701, 708, 715, 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 806 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 97 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90867 T 105 MASS(ES) DORSAL WEEK 64 442, 449, 456, 463, 470, 477, 484, 491C, 498C, 505C, 512C, 519C 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 731C C90868 M 73 APPEARANCE HUNCHED 508 BEHAVIOR RECUMBENT LATERAL 508 EXCRETION NO FECES 508 RESPIRATION IRREGULAR 508 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL-LEFT SORE/SCAB HEAD-MAXILLARY-RIGHT LIMB-HIND-LEFT PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND YELLOW HAIR COAT 508 365, 372 323, 330, 337, 344 351, 358, 365, 372, 379 477, 484, 491, 498, 505 508 508 807 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 98 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90868 M 73 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 386, 393, 400, 407, 414, 421, 428, 435, 442 449, 456, 463, 470 C90869 T 105 APPEARANCE SWOLLEN PAWS-HIND 540, 547, 554, 561 SKIN & PELAGE SORE/SCAB SCROTUM SCROTUM-RIGHT 694, 701, 708, 715, 722, 729-730 568, 575 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 610, 617, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 624, 631, 638, 645, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 582, 652, 659, 666, 673, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 589, 596, 603 680, 687 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 561, 568, 575 808 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 99 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90870 M 80 APPEARANCE THIN 547, 554 BEHAVIOR HYPOACTIVE 554 EXCRETION FEW FECES 547, 554 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT 526, 533, 540, 547, 554 547, 554 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C90871 T 105 EXCRETION NONFORMED FECES 162, 169, 176, 183, 211, 218 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 246, 253, 337, 344, 428, 435, 519, 526, 22, 29, 36, 43, 50, 127, 134, 141, 148, 260, 267, 274, 281, 351, 358, 365, 372, 442, 449, 456, 463, 533, 540, 547, 554, 57, 64, 71, 78, 85, 155, 190, 197, 204, 288, 295, 302, 309, 379, 386, 393, 400, 470, 477, 484, 491, 561, 568, 575, 582, 92, 99, 106 225, 232, 239 316, 323, 330 407, 414, 421 498, 505, 512 589, 596, 603 809 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 100 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90871 T 105 NORMAL NO REMARKABLE OBSERVATIONS 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694 701, 708, 715, 722, 729-730 C90872 D 91 EXCRETION FEW FECES NONFORMED FECES 631, 637 155 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 211, 218, 302, 309, 393, 400, 484, 491, 575, 582, 22, 29, 36, 43, 50, 127, 134, 141, 148, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 589, 596, 603, 610, 57, 64, 71, 78, 85, 162, 169, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 617, 624 92, 99, 106 190, 197, 204 281, 288, 295 372, 379, 386 463, 470, 477 554, 561, 568 C90873 M 73 APPEARANCE HEAD TILT RIGHT 507-508 BEHAVIOR ATAXIC 507-508 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL VENTRAL-THORACIC SORE/SCAB PAW-HIND-LEFT 507-508 507-508 470, 477, 484, 491, 498, 505, 507-508 810 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 101 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90873 M 73 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463 C90874 M 67 APPEARANCE THIN 463 EXCRETION FEW FECES NONFORMED FECES 393, 400, 407, 437, 442, 449, 456, 463 337 EYES RED DISCHARGE EYES 393, 463 RESPIRATION AUDIBLE LABORED 393, 437, 463 393, 463 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 414, 421, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 428, 435 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 344, 351, 358, 365, 92, 99, 106 183, 190, 197 274, 281, 288 372, 379, 386 811 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 102 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90875 D 91 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 274, 281, 365, 372, 456, 463, 547, 554, 631-632 22, 29, 36, 43, 50, 127, 134, 141, 218, 288, 295, 302, 309, 379, 386, 393, 400, 470, 477, 484, 491, 561, 568, 575, 582, 57, 64, 71, 78, 85, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 589, 596, 603, 610, 92, 99, 106 253, 260, 267 344, 351, 358 435, 442, 449 526, 533, 540 617, 624 WART LIKE LESION(S) TAIL WEEK 22 148, 155, 162, 169, 176, 183, 190, 197, 204, 211 C90876 A 56 APPEARANCE MALOCCLUSION PARALYSIS LIMBS-HIND 92, 99, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169, 176 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 274 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 372, 379, 386 386 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 267 C90877 3 14 APPEARANCE MALOCCLUSION 64, 71, 78, 85, 92, 94 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57 812 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 103 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90878 D 53 SKIN & PELAGE SORE/SCAB DORSAL-CERVICAL-RIGHT EAR-RIGHT VENTRAL-CERVICAL-LEFT VENTRAL-CERVICAL-RIGHT 260, 267, 274, 281, 218, 225 15, 22, 29, 36, 43, 120, 127, 134 141, 148, 155, 162, 288, 295, 302, 309, 50, 57, 64, 71, 78, 169, 176, 183, 190, 316, 323, 330, 337 85, 92, 99, 106, 113 197, 204, 211 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 232, 239, 246, 253, 344, 351, 358, 365, 369 C90879 M 81 APPEARANCE HUNCHED THIN 561 547, 554, 561 EXCRETION FEW FECES NO FECES 554, 561 545-547 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA YELLOW HAIR COAT 547 545-547 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 813 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 104 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90880 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C90881 T 105 DISCHARGE NASAL RED IN COLOR 680 EXCRETION NONFORMED FECES 533, 582 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 575, 589, 673, 687, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 596, 603, 610, 617, 694, 701, 708, 715, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 540, 547, 624, 631, 638, 645, 722, 729, 731 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 554, 561, 568 652, 659, 666 C90882 T 105 DISCHARGE GENITAL RED IN COLOR 253, 708 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 302, 309, 393, 400, 484, 491, 575, 582, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 589, 596, 603, 610, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 617, 624, 631, 638, 92, 99, 106 183, 190, 197 281, 288, 295 372, 379, 386 463, 470, 477 554, 561, 568 645, 652, 659 814 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90882 T 105 NORMAL NO REMARKABLE OBSERVATIONS 666, 673, 680, 687, 694, 701, 715, 722, 729, 731 C90883 T 105 APPEARANCE MALOCCLUSION 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708, 715, 722, 729, 732 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218 C90884 T 105 EYES RED DISCHARGE EYE-LEFT 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 400 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729, 731 815 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 106 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90884 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533, 540 C90885 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 649, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C90886 M 91 APPEARANCE MALOCCLUSION THIN 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547, 554 589, 596, 603, 610, 617, 624, 630-631 EXCRETION FEW FECES 630-631 EYES SQUINTED PERI-ORBITAL 630-631 816 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 107 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90886 M 91 SKIN & PELAGE PALE ENTIRE BODY 630-631 SORE/SCAB PAW-HIND-RIGHT 603, 610, 617, 624, 630-631 TAIL 547, 554 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 582 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 561, 568, 575 WART LIKE LESION(S) PAW-HIND-LEFT WEEK 89 617, 624, 630-631 C90887 M 69 APPEARANCE HUNCHED 477 BEHAVIOR HYPOACTIVE 477 EXCRETION NO FECES 477 EYES SQUINTED PERI-ORBITAL 477 817 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 108 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90887 M 69 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL 470 RED HAIR COAT HEAD-ENTIRE 477 VENTRAL-ABDOMINAL 477 VENTRAL-CERVICAL 477 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463 C90888 M 81 APPEARANCE MALOCCLUSION THIN 43, 50, 57, 64, 71, 148, 155, 162, 169, 239, 246, 253, 260, 330, 337, 344, 351, 421, 428, 435, 442, 512, 519, 526, 533, 558, 561 78, 85, 92, 99, 106, 113, 120, 127, 134, 141 176, 183, 190, 197, 204, 211, 218, 225, 232 267, 274, 281, 288, 295, 302, 309, 316, 323 358, 365, 372, 379, 386, 393, 400, 407, 414 449, 456, 463, 470, 477, 484, 491, 498, 505 540, 547, 554, 558, 561 EXCRETION FEW FECES NO FECES 547, 554, 558 561 SKIN & PELAGE RED HAIR COAT PERINEAL AREA 561 818 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 109 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90888 M 81 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36 WART LIKE LESION(S) DORSAL WEEK 79 547, 554, 558, 561 C90889 T 105 EXCRETION NONFORMED FECES 218, 582, 701 SKIN & PELAGE SORE/SCAB LATERAL-ABDOMINAL-LEFT LIMB-HIND-LEFT PAW-FRONT-RIGHT PAW-HIND-LEFT 701, 708 701, 708 547 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 302, 309, 393, 400, 484, 491, 22, 29, 36, 43, 50, 127, 134, 141, 148, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519 57, 64, 71, 78, 85, 155, 162, 169, 176, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 92, 99, 106 183, 190, 197 281, 288, 295 372, 379, 386 463, 470, 477 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 526, 533, 540, 547, 554, 561, 568, 575C 819 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 110 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90890 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C90891 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C90892 M 77 APPEARANCE THIN 526, 532-533 BEHAVIOR HYPOACTIVE 532-533 EXCRETION FEW FECES NO FECES 526 532-533 EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL-RIGHT 526, 532-533 526, 532-533 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 526, 532-533 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 820 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 111 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90892 M 77 NORMAL NO REMARKABLE OBSERVATIONS 477, 484, 491, 498, 505, 512, 519 C90893 T 105 EXCRETION NONFORMED FECES 715, 722 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 729, 731 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C90894 M 98 BEHAVIOR HYPOACTIVE 684 RECUMBENT STERNAL 684 RESPIRATION LABORED 684 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA RED HAIR COAT HEAD-ENTIRE 680, 684 568, 575, 582, 680, 684 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 821 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 112 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90894 M 98 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 C90895 D 94 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 657 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C90896 M 72 APPEARANCE HUNCHED MALOCCLUSION MISSING TEETH THIN 477, 484, 491, 498 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106, 113, 120, 127, 134, 141, 148 183, 190, 197, 204, 211, 218, 225, 232, 239 274, 281, 288, 295, 302, 309, 316, 323, 330 365, 372, 379, 386, 393, 400, 407, 414, 421 456, 463, 470, 477, 484, 491, 498 190, 197, 204, 211, 218, 225, 232, 239, 246 477, 484, 491, 498 BEHAVIOR HYPOACTIVE 477, 484, 491, 498 822 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 113 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90896 M 72 EXCRETION FEW FECES 498 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C90897 D 95 EYES RED DISCHARGE EYE-LEFT 22, 29 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113, 120 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 661 MASS(ES) MOUTH WEEK 82 568 C90898 M 62 APPEARANCE THIN 428, 431 BEHAVIOR HYPOACTIVE 431 EXCRETION NO FECES 431 823 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 114 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90898 M 62 EYES SQUINTED PERI-ORBITAL 428, 431 SKIN & PELAGE PALE ENTIRE BODY RED HAIR COAT HEAD-ENTIRE 431 428, 431 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421 C90899 M 104 APPEARANCE PROTRUDING PENIS 680, 687, 694 BEHAVIOR HYPOACTIVE 722 RECUMBENT LATERAL 722 EXCRETION FEW FECES 715, 722 EYES RED DISCHARGE EYES 722 824 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 115 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90899 M 104 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 568, 575, 582 VENTRAL-ABDOMINAL 722 SORE/SCAB DORSAL 708 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 WART LIKE LESION(S) DORSAL WEEK 69 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701 C90900 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 731 825 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 116 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C90841 M3 575 83 MASS(ES) VENTRAL-ABDOMINAL WEEK 83 10 X 10MM C90867 M3 491 71 TO 624 90 MASS(ES) DORSAL WEEK 64 15 X 15MM 631 91 TO 731 105 MASS(ES) DORSAL WEEK 64 20 X 20MM C90889 M3 575 83 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 10 X 10MM 826 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 117 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90901 D 74 APPEARANCE HUNCHED 510, 512 EXCRETION FEW FECES 510, 512 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C90902 4 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C90903 D 101 APPEARANCE MALOCCLUSION 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 659, 666, 673 680, 687, 694, 701, 703 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 638, 645, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 652 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 631 827 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 118 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90904 T 105 DISCHARGE NASAL RED IN COLOR 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C90905 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C90906 M 102 APPEARANCE HUNCHED 713 THIN 713 BEHAVIOR HYPOACTIVE 713 EXCRETION NO FECES 713 EYES RED DISCHARGE EYES 708, 713 828 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 119 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90906 M 102 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL 708, 713 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C90907 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C90908 T 105 APPEARANCE MALOCCLUSION 155, 162, 169, 176, 302, 309, 316, 323, 330, 337, 344, 351, 358 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 183, 190, 197, 204, 211, 218, 225 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 829 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 120 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90909 4 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C90910 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 732 C90911 T 105 EXCRETION NONFORMED FECES 575, 582 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 589, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 596, 603, 610, 617, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 624, 631, 638, 645, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 652 WART LIKE LESION(S) MOUTH WEEK 95 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 830 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 121 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90912 M 82 APPEARANCE HUNCHED THIN 561, 564, 568 547, 554, 561, 564, 568 BEHAVIOR ATAXIC SENSITIVE TO TOUCH 564, 568 564, 568 DISCHARGE ORAL RED IN COLOR 498 EYES SQUINTED PERI-ORBITAL 547, 554, 561, 564, 568 SKIN & PELAGE PALE ENTIRE BODY YELLOW HAIR COAT 564, 568 568 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 505, 512, 519, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 526, 533, 540 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 831 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 122 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90913 4 53 APPEARANCE MALOCCLUSION MISSING TEETH 50, 57, 64, 71, 78, 85, 92, 99, 106, 113, 120, 127, 134, 141 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323 330, 337, 344, 351, 358, 365, 367 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316 323, 330, 337, 344, 351, 358, 365, 367 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43 C90914 M 100 APPEARANCE HUNCHED 692, 694, 696 EXCRETION FEW FECES 692, 694, 696 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 659, 666, 673, 680, 687, 692, 694, 696 659, 666, 673, 680, 687, 692, 694, 696 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 832 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 123 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90914 M 100 MASS(ES) LIMB-HIND-LEFT WEEK 76 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C, 692C, 694C, 696C C90915 D 96 DISCHARGE NASAL RED IN COLOR 344, 659, 666 SKIN & PELAGE SORE/SCAB HEAD-MAXILLARY-RIGHT VENTRAL-ABDOMINAL 631, 638 505 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 393, 400, 484, 491, 582, 589, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 407, 414, 421, 428, 498, 512, 519, 526, 596, 603, 610, 617, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 351, 358, 365, 435, 442, 449, 456, 533, 540, 547, 554, 624, 645, 652 92, 99, 106 183, 190, 197 274, 281, 288 372, 379, 386 463, 470, 477 561, 568, 575 C90916 T 105 EXCRETION NONFORMED FECES 589, 596 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 833 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 124 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90916 T 105 NORMAL NO REMARKABLE OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 C90917 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C90918 4 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C90919 D 79 APPEARANCE MALOCCLUSION SWOLLEN PAWS-FRONT 477 526, 533, 540, 547, 551 SKIN & PELAGE SORE/SCAB EARS 526, 533, 540, 547, 551 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 484, 491, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 498, 505, 512, 519 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 834 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 125 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90920 M 104 APPEARANCE HUNCHED 727 BEHAVIOR HYPOACTIVE 727 DISCHARGE NASAL RED IN COLOR 727 EXCRETION NONFORMED FECES 533, 575, 582, 727 EYES SQUINTED PERI-ORBITAL 727 RESPIRATION LABORED 727 SKIN & PELAGE SORE/SCAB SCROTUM TAIL 701, 708, 715, 722, 727 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687 694, 701 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 835 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 126 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90920 M 104 NORMAL NO REMARKABLE OBSERVATIONS 477, 484, 491, 498, 505, 512, 519, 526, 540, 547, 554, 561, 568 589, 596 C90921 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C90922 M 64 APPEARANCE THIN 442 BEHAVIOR HYPOACTIVE 442 DISCHARGE NASAL RED IN COLOR 414 EXCRETION FEW FECES NONFORMED FECES 414, 421, 428, 435, 442 155 EYES RED DISCHARGE EYES 414, 428, 435, 442 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL YELLOW HAIR COAT 414 428, 435, 442 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 836 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 127 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90922 M 64 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 162, 169, 176, 183, 190, 225, 232 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 29 197, 204, 211, 218 C90923 M 86 APPEARANCE THIN 594, 596, 601 BEHAVIOR HYPOACTIVE 594, 596, 601 DISCHARGE NASAL RED IN COLOR 589, 594, 596, 601 EXCRETION FEW FECES 594, 596, 601 SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL SORE/SCAB VENTRAL-ABDOMINAL 589, 594, 596, 601 365, 372 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 295, 302, 309, 316, 323, 330 837 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 128 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90923 M 86 NORMAL NO REMARKABLE OBSERVATIONS 337, 344, 351, 358, 379, 386, 393, 400, 407, 414, 421, 428, 435 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526 533, 540, 547, 554, 561, 568, 575, 582 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 37 253, 260C, 267C, 274C, 281C, 288C C90924 4 53 APPEARANCE MALOCCLUSION 197, 204, 211, 260, 267, 295, 302, 309, 316, 323, 330, 337, 365 367 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 218 225, 232, 239, 246, 253, 274, 281, 288, 344, 351, 358 C90925 M 93 BEHAVIOR HYPOACTIVE RECUMBENT STERNAL 645-646 645-646 DISCHARGE GENITAL RED IN COLOR 646 NASAL RED IN COLOR 646 EXCRETION FEW FECES 645-646 838 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 129 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90925 M 93 EYES RED DISCHARGE EYES 645-646 SKIN & PELAGE YELLOW HAIR COAT 645-646 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638 C90926 T 105 APPEARANCE THIN 728-730 EXCRETION FEW FECES 728-730 EYES RED DISCHARGE EYES 728-730 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT YELLOW HAIR COAT 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708 728-730 839 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 130 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90926 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 295, 302, 309, 316, 323 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 715 722 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 38 260, 267, 274, 281, 288 C90927 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 631, 638, 645, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 652 MASS(ES) AXILLARY REGION-RIGHT WEEK 89 VENTRAL-THORACIC-LEFT WEEK 95 617C, 624C 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 729-730C 840 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 131 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90928 T 105 APPEARANCE MALOCCLUSION THIN 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694, 701, 708, 715, 722, 729-730 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 722, 729-730 EYES DISCOLORED/LIGHT RED EYES 582, 589, 596 RESPIRATION AUDIBLE 582, 589, 596 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 582, 589, 596 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 841 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 132 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90929 D 69 APPEARANCE MALOCCLUSION 71, 78, 92, 99, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 449, 456, 463, 470, 477 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 85 C90930 M 90 APPEARANCE CONVULSIONS MALOCCLUSION MISSING TEETH PROTRUDING PENIS 568 43, 50, 57, 64, 71, 148, 155, 162, 169, 239, 246, 253, 260, 351, 358, 365, 372, 442, 449, 456, 463, 533, 540, 547, 554, 624 78, 85, 92, 99, 106, 113, 120, 127, 134, 141 176, 183, 190, 197, 204, 211, 218, 225, 232 288, 295, 302, 309, 316, 323, 330, 337, 344 379, 386, 393, 400, 407, 414, 421, 428, 435 470, 477, 484, 491, 498, 505, 512, 519, 526 561, 568, 575, 582, 589, 596, 603, 610, 617 568, 575, 582, 589, 596, 603, 610, 617, 624 589 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL 582, 589, 596, 603, 610, 617, 624 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 267, 274, 281 842 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 133 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90931 M 98 APPEARANCE LIMITED USE LIMBS-HIND 680 PAWS-FRONT 680 BEHAVIOR ATAXIC 680 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C90932 4 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C90933 T 105 APPEARANCE MALOCCLUSION 78, 85, 92, 99, 106, 134, 141, 148, 155, 162, 169, 176, 183, 190 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 302 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694, 701, 708, 715, 722, 729-730 843 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 134 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90933 T 105 DISCHARGE GENITAL RED IN COLOR 372, 379, 533 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 113, 120, 127, 281 288, 295 MASS(ES) AXILLARY REGION-LEFT WEEK 88 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C 687C, 694C, 701C, 708C, 715C, 722C, 729-730C C90934 M 103 APPEARANCE HUNCHED THIN 716, 718-719 718-719 BEHAVIOR HYPOACTIVE 719 DISCHARGE ORAL RED IN COLOR 716, 718-719 EXCRETION FEW FECES 715-716, 718-719 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL RED HAIR COAT HEAD-ENTIRE 715-716, 718-719 716, 718-719 844 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 135 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90934 M 103 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701 MASS(ES) VENTRAL-ABDOMINAL WEEK 102 708C, 715-716C, 718-719C C90935 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C90936 M 101 APPEARANCE MALOCCLUSION THIN 43, 50, 57, 64, 71, 148, 155, 162, 169, 239, 246, 253, 260, 330, 337, 344, 351, 421, 428, 435, 442, 512, 519, 526, 533, 603, 610, 617, 624, 694, 701, 703, 705 705 78, 85, 92, 99, 106, 113, 120, 127, 134, 141 176, 183, 190, 197, 204, 211, 218, 225, 232 267, 274, 281, 288, 295, 302, 309, 316, 323 358, 365, 372, 379, 386, 393, 400, 407, 414 449, 456, 463, 470, 477, 484, 491, 498, 505 540, 547, 554, 561, 568, 575, 582, 589, 596 631, 638, 645, 652, 659, 666, 673, 680, 687 BEHAVIOR HYPOACTIVE 705 845 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 136 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90936 M 101 EXCRETION DISCOLORED URINE RED IN COLOR 705 FEW FECES 703 NO FECES 705 SKIN & PELAGE PALE ENTIRE BODY ROUGH HAIR COAT SORE/SCAB PAW-HIND-LEFT PAWS-HIND 705 703, 705 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 703, 705 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36 C90937 M 103 APPEARANCE HUNCHED MALOCCLUSION 715 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715 92, 99, 106, 113, 120, 127, 134, 141, 148 183, 190, 197, 204, 211, 218, 225, 232, 239 274, 281, 288, 295, 302, 309, 316, 323, 330 365, 372, 379, 386, 393, 400, 407, 414, 421 456, 463, 470, 477, 484, 491, 498, 505, 512 547, 554, 561, 568, 575, 582, 589, 596, 603 638, 645, 652, 659, 666, 673, 680, 687, 694 EXCRETION NO FECES 715 846 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 137 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90937 M 103 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA 715 VENTRAL-ABDOMINAL 715 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C90938 M 69 APPEARANCE HUNCHED 477, 481 BEHAVIOR HYPOACTIVE 481 RECUMBENT LATERAL 481 EXCRETION FEW FECES 477 SKIN & PELAGE BROWN HAIR COAT HEAD-MAXILLARY-LEFT 481 HEAD-MAXILLARY-RIGHT 481 BROWN SKIN VENTRAL-ABDOMINAL 481 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 847 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 138 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90939 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C90940 M 64 APPEARANCE HEAD TILT LEFT HUNCHED THIN 442 440, 442 440, 442 BEHAVIOR ATAXIC HYPOACTIVE 440, 442 440, 442 EXCRETION NO FECES 440, 442 EYES RED DISCHARGE EYES 440, 442 RESPIRATION LABORED 440, 442 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL 440, 442 440, 442 435, 440, 442 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 848 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 139 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90940 M 64 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428 C90941 M 88 APPEARANCE HUNCHED THIN 610 589, 596, 603, 609-610 BEHAVIOR HYPOACTIVE 610 EXCRETION FEW FECES 561, 568, 575, 582, 589, 596, 603, 609-610 EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL 610 609-610 SKIN & PELAGE COLD TO TOUCH ENTIRE BODY 610 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 849 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 140 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90941 M 88 NORMAL NO REMARKABLE OBSERVATIONS 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554 C90942 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C90943 D 92 APPEARANCE MALOCCLUSION SWOLLEN MOUTH THIN 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 629, 92, 99, 106, 113, 120, 127, 134, 141, 148 183, 190, 197, 204, 211, 218, 225, 232, 239 274, 281, 288, 295, 302, 309, 316, 323, 330 365, 372, 379, 386, 393, 400, 407, 414, 421 456, 463, 470, 477, 484, 491, 498, 505, 512 547, 554, 561, 568, 575, 582, 589, 596, 603 631, 638-639 57, 64 631, 638-639 BEHAVIOR HYPOACTIVE 631, 638-639 EXCRETION FEW FECES NO FECES 629, 631 638-639 SKIN & PELAGE YELLOW HAIR COAT 629, 631, 638-639 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 850 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 141 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90944 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 731 C90945 M 45 DISCHARGE NASAL RED IN COLOR 309 RESPIRATION AUDIBLE 309 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL 309 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302 851 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 142 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90946 M 78 APPEARANCE MALOCCLUSION THIN 50, 57, 64, 71, 78, 85, 92, 99, 106, 113, 120, 127, 134, 141 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505 512, 518-519, 526, 533, 540 518-519, 526, 533, 540 EYES RED DISCHARGE EYE-LEFT 540 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43 C90947 T 105 SKIN & PELAGE SORE/SCAB DORSAL-THORACIC-RIGHT 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554 852 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 143 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90948 T 105 APPEARANCE MALOCCLUSION 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708, 715, 722, 729, 731 EYES RED DISCHARGE EYES 127 SKIN & PELAGE SORE/SCAB PAWS-HIND 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120 C90949 M 13 APPEARANCE MALOCCLUSION THIN 71, 78, 85-86 78, 85-86 EXCRETION NONFORMED FECES 85-86 853 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 144 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90949 M 13 SKIN & PELAGE BROWN HAIR COAT SCROTUM 85-86 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64 C90950 4 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C90951 4 53 APPEARANCE MALOCCLUSION MISSING TEETH 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 120, 127, 134, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 EYES RED DISCHARGE EYE-RIGHT 29, 36, 43 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113 854 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 145 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90952 M 59 BEHAVIOR RECUMBENT LATERAL 411 EXCRETION FEW FECES 407, 411 EYES RED DISCHARGE EYE-LEFT SQUINTED PERI-ORBITAL 386 407, 411 RESPIRATION AUDIBLE 411 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 393, 400 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 C90953 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729-730 855 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 146 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90954 M 69 APPEARANCE THIN 477, 479 EXCRETION NO FECES 479 SKIN & PELAGE ROUGH HAIR COAT YELLOW HAIR COAT 225, 477, 479 463, 470, 477, 479 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 C90955 T 105 APPEARANCE SWOLLEN TAIL-PROXIMAL 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 856 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 147 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90956 T 105 APPEARANCE HUNCHED MALOCCLUSION THIN 727, 729, 731 71, 78, 85, 92, 99, 106, 113, 120, 127, 134, 141, 148, 155, 162 169, 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708 715, 722, 727, 729, 731 727, 729, 731 EXCRETION NO FECES 727, 729, 731 EYES SQUINTED PERI-ORBITAL 729, 731 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL 727, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64 857 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 148 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90957 4 53 APPEARANCE MALOCCLUSION MISSING TEETH 50, 57, 64, 71, 78, 85, 92, 99, 106, 113, 120, 127, 134, 141 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323 330, 337, 344, 351, 358, 365, 367 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43 C90958 D 94 APPEARANCE MALOCCLUSION SWOLLEN PAW-HIND-LEFT 50, 57, 64, 71, 78, 148, 155, 162, 169, 239, 246, 253, 260, 330, 337, 344, 351, 421, 428, 435, 442, 512, 519, 526, 533, 603, 610, 617, 624, 85, 92, 99, 106, 113, 120, 127, 134, 141 176, 183, 190, 197, 204, 211, 218, 225, 232 267, 274, 281, 288, 295, 302, 309, 316, 323 358, 365, 372, 379, 386, 393, 400, 407, 414 449, 456, 463, 470, 477, 484, 491, 498, 505 540, 547, 554, 561, 568, 575, 582, 589, 596 631, 638, 645, 652, 656 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 656 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435 442, 449, 456, 463, 470 526, 533, 540, 547 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 656 858 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 149 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90958 D 94 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43 C90959 M 86 APPEARANCE HEAD TILT LEFT 600 BEHAVIOR ATAXIC 600 EXCRETION FEW FECES 600 EYES RED DISCHARGE EYE-RIGHT 600 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 859 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 150 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90960 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C90961 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C90962 M 71 APPEARANCE HUNCHED MALOCCLUSION THIN 477, 484, 488-489, 491-492 295 491-492 BEHAVIOR HYPOACTIVE 488-489, 491-492 EXCRETION NO FECES 491-492 EYES SQUINTED PERI-ORBITAL 488-489, 491-492 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL ROUGH HAIR COAT 488-489, 491-492 477, 484, 488-489, 491-492 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 302, 309, 393, 400, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 316, 323, 330, 337, 407, 414, 421, 428, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 344, 351, 358, 365, 435, 442, 449, 456, 92, 99, 106 183, 190, 197 274, 281, 288 372, 379, 386 463, 470 860 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 151 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90963 M 73 APPEARANCE HUNCHED THIN 477, 484, 509 498, 505, 507, 509 BEHAVIOR ATAXIC HYPOACTIVE 509 507, 509 SKIN & PELAGE COLD TO TOUCH ENTIRE BODY ROUGH HAIR COAT SORE/SCAB HEAD-MAXILLARY-LEFT YELLOW HAIR COAT 507, 509 477, 484, 491, 498, 505, 507, 509 386, 393, 400 507, 509 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 407, 414, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 421, 428, 435, 442, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 449, 456, 463, 470 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 C90964 M 74 APPEARANCE MALOCCLUSION PARALYSIS LIMB-FRONT-LEFT SWOLLEN PAWS-HIND THIN 99, 106, 113, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 456, 463, 470 120 505, 512, 515 512, 515 861 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 152 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90964 M 74 EYES DISCOLORED/LIGHT RED EYE-LEFT 512, 515 OPAQUE EYE-RIGHT 512, 515 SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB HEAD-MAXILLARY-RIGHT MOUTH PAW-HIND-RIGHT PAWS-HIND 515 8 477, 484 302, 309, 316, 323, 330, 337, 344 351, 358, 365, 372, 379, 477, 484, 491, 498, 505, 512, 515 NORMAL NO REMARKABLE OBSERVATIONS 1, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 127, 134, 141 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232 239, 246, 253, 260, 267, 274, 281, 288 MASS(ES) MOUTH WEEK 67 463, 470 C90965 T 105 APPEARANCE MALOCCLUSION 50, 57, 64, 71, 78, 148, 155, 162, 169, 239, 246, 253, 260, 330, 337, 344, 351, 421, 428, 435, 442, 512, 519, 526, 533, 603, 610, 617, 624, 85, 92, 99, 106, 113, 120, 127, 134, 141 176, 183, 190, 197, 204, 211, 218, 225, 232 267, 274, 281, 288, 295, 302, 309, 316, 323 358, 365, 372, 379, 386, 393, 400, 407, 414 449, 456, 463, 470, 477, 484, 491, 498, 505 540, 547, 554, 561, 568, 575, 582, 589, 596 631, 638, 645, 652, 659, 666, 673, 680, 687 862 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 153 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90965 T 105 APPEARANCE MALOCCLUSION 694, 701, 708, 715, 722, 729, 731 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT PAW-HIND-RIGHT 729, 731 568, 575, 582, 589, 596 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43 C90966 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C90967 4 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C90968 T 105 APPEARANCE MALOCCLUSION THIN 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708, 715, 722, 729-730 477 EXCRETION NO FECES 477 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL 477, 484 477 863 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 154 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90968 T 105 SKIN & PELAGE SORE/SCAB DORSAL-THORACIC-LEFT 477 PAWS-HIND 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729-730 VENTRAL-ABDOMINAL 477 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C90969 D 85 APPEARANCE THIN 568, 575 BEHAVIOR HYPOACTIVE 568 EXCRETION FEW FECES 568, 575 EYES SQUINTED PERI-ORBITAL 568 SKIN & PELAGE BROWN HAIR COAT GENERALIZED 568, 575 864 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 155 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90969 D 85 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 540, 547, 554, 561, 568, 575, 582, 589, 593 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C90970 M 100 BEHAVIOR HYPOACTIVE 697 EXCRETION NONFORMED FECES 344 EYES SQUINTED PERI-ORBITAL 697 RESPIRATION IRREGULAR 697 SKIN & PELAGE COLD TO TOUCH ENTIRE BODY SORE/SCAB DORSAL PAW-HIND-LEFT 697 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 697 666, 673, 680, 687, 694, 697 865 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 156 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C90970 M 100 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 351, 358, 365, 372, 379 MASS(ES) DORSAL WEEK 56 386C, 463C, 540C, 617C, 694C, 393C, 470C, 547C, 624C, 697C 400C, 477C, 554C, 631C, 407C, 484C, 561C, 638C, 414C, 491C, 568C, 645C, 421C, 498C, 575C, 652C, 428C, 505C, 582C, 659C, 435C, 512C, 589C, 666C, 442C, 519C, 596C, 673C, 449C, 526C, 603C, 680C, 456C 533C 610C 687C 866 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 157 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C90914 M4 526 76 MASS(ES) LIMB-HIND-LEFT WEEK 76 50X40MM 533 77 TO 568 82 MASS(ES) LIMB-HIND-LEFT WEEK 76 50 X 50MM 575 83 TO 696 100 MASS(ES) LIMB-HIND-LEFT WEEK 76 65 X 60MM C90923 M4 260 38 TO 288 42 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 37 10X 10MM C90927 M4 617 89 TO 624 90 MASS(ES) AXILLARY REGION-RIGHT WEEK 89 10 X 10MM 659 95 TO 701 101 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 95 40 X 30MM 708 102 TO 722 104 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 95 55 X 60MM 729 105 TO 730 105 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 95 70 X 65MM 867 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 158 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C90933 M4 610 88 TO 624 90 MASS(ES) AXILLARY REGION-LEFT WEEK 88 10 X 10MM 631 91 TO 638 92 MASS(ES) AXILLARY REGION-LEFT WEEK 88 35 X 40MM 645 93 MASS(ES) AXILLARY REGION-LEFT WEEK 88 40 X 40MM 652 94 MASS(ES) AXILLARY REGION-LEFT WEEK 88 60 X 60MM 659 95 TO 687 99 MASS(ES) AXILLARY REGION-LEFT WEEK 88 80 X 60MM 694 100 TO 701 101 MASS(ES) AXILLARY REGION-LEFT WEEK 88 100 X 70MM 708 102 TO 715 103 MASS(ES) AXILLARY REGION-LEFT WEEK 88 100 X 110MM 722 104 MASS(ES) AXILLARY REGION-LEFT WEEK 88 110 X 110MM 868 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 159 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C90933 M4 729 105 TO 730 105 MASS(ES) AXILLARY REGION-LEFT WEEK 88 120 X 110MM C90934 M4 708 102 TO 719 103 MASS(ES) VENTRAL-ABDOMINAL WEEK 102 25 X 40MM C90970 M4 386 56 TO 484 70 MASS(ES) DORSAL WEEK 56 10 X 10MM 491 71 TO 526 76 MASS(ES) DORSAL WEEK 56 15 X 15MM 533 77 TO 697 100 MASS(ES) DORSAL WEEK 56 20 X 25MM 869 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 160 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90971 2 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 54 C90972 2 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 54 C90973 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C90974 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C90975 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C90976 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C90977 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C90978 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C90979 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 870 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 161 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90980 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C90981 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C90982 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C90983 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C90984 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C90985 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C90986 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C90987 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C90988 2 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 54 871 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 162 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90989 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C90990 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C90991 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C90992 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C90993 D 8 APPEARANCE MALOCCLUSION 50, 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43 C90994 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C90995 6 8 SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL 15, 22, 29, 36, 43, 50 NORMAL NO REMARKABLE OBSERVATIONS 1, 8 872 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 163 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C90996 2 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 54 C90997 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C90998 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C90999 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91000 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91001 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91002 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91003 D 7 APPEARANCE CONVULSIONS 43 BEHAVIOR HYPOACTIVE 43 SKIN & PELAGE COLD TO TOUCH ENTIRE BODY 43 PALE ENTIRE BODY 43 873 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 164 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91003 D 7 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 45 C91004 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91005 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91006 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91007 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91008 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91009 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91010 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91011 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 874 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 165 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91012 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91013 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91014 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91015 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91016 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91017 2 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 54 C91018 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91019 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91020 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 875 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 166 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91021 6 8 APPEARANCE MALOCCLUSION THIN 46, 50 50 DISCHARGE NASAL RED IN COLOR 46, 50 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43 C91022 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91023 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91024 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91025 6 8 NORMAL NO REMARKABLE OBSERVATIONS 8, 15, 22, 29, 36, 43, 50 C91026 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 876 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 167 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91027 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91028 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91029 M 7 APPEARANCE CONVULSIONS 49 BEHAVIOR VOCALIZATION 49 DISCHARGE UNKNOWN RED IN COLOR 49 RESPIRATION LABORED 49 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 49 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43 877 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 168 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91030 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91031 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91032 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91033 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91034 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91035 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91036 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91037 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91038 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 878 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 169 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91039 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91040 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 879 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 170 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91041 M 61 APPEARANCE THIN 421 EXCRETION FEW FECES 407, 414, 421 EYES SQUINTED PERI-ORBITAL 414, 421 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL RED HAIR COAT VENTRAL-CERVICAL ROUGH HAIR COAT 407, 414, 421 421 421 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 C91042 D 93 APPEARANCE CONVULSIONS 603, 610 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 638, 645, 650 880 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 171 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91042 D 93 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 617, 624, 631 C91043 M 85 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C 589-590C C91044 M 64 APPEARANCE HEAD TILT RIGHT HUNCHED THIN 443 443 435, 442-443 DISCHARGE ORAL CLEAR 57 881 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 172 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91044 M 64 DISCHARGE NASAL RED IN COLOR 183 EXCRETION FEW FECES 443 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 120, 127, 218, 225, 309, 316, 400, 407, 22, 29, 36, 43, 50, 134, 141, 148, 155, 232, 239, 246, 253, 323, 330, 337, 344, 414, 421, 428 64, 71, 78, 85, 92, 162, 169, 176, 190, 260, 267, 274, 281, 351, 358, 365, 372, 99, 106, 113 197, 204, 211 288, 295, 302 379, 386, 393 C91045 M 51 APPEARANCE PARALYSIS LIMB-FRONT-LEFT 120 BEHAVIOR HYPOACTIVE 351, 353 EXCRETION FEW FECES NONFORMED FECES 351, 353 351 EYES RED DISCHARGE EYE-LEFT EYES 92, 99, 106, 113, 281, 288, 295, 302, 309, 316, 323, 330, 337 344 351, 353 882 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 173 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91045 M 51 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL 351, 353 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 127, 134, 141 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232 239, 246, 253, 260, 267, 274 C91046 M 7 EYES RED DISCHARGE EYE-LEFT 43-44 RESPIRATION IRREGULAR 43-44 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36 C91047 U 105 APPEARANCE MALOCCLUSION 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106, 113, 120, 127, 134, 141, 148 183, 190, 197, 204, 211, 218, 225, 232, 239 274, 281, 288, 295, 302, 309, 316, 323, 330 365, 372, 379, 386, 393, 400, 407, 414, 421 456, 463, 470, 477, 484, 491, 498, 505, 512 547, 554, 561, 568, 575, 582, 589, 596, 603 638, 645, 652, 659, 666, 673, 680, 687, 694 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 883 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 174 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91048 M 80 APPEARANCE THIN 547, 554 BEHAVIOR HYPOACTIVE 547, 554 EXCRETION FEW FECES 547 NO FECES 554 EYES SQUINTED PERI-ORBITAL 547, 554 SKIN & PELAGE BROWN HAIR COAT GENERALIZED 547, 554 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 884 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 175 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91049 D 43 APPEARANCE SWOLLEN VENTRAL-ABDOMINAL 288, 295 BEHAVIOR RECUMBENT LATERAL 295 DISCHARGE NASAL RED IN COLOR 295 EXCRETION FEW FECES NO FECES 288, 295 281 RESPIRATION IRREGULAR 295 SKIN & PELAGE ROUGH HAIR COAT 295 YELLOW HAIR COAT 295 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274 885 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 176 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91050 D 103 APPEARANCE MALOCCLUSION 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708, 715, 719 SKIN & PELAGE YELLOW HAIR COAT 652, 659, 666, 673, 680, 687, 694 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 WART LIKE LESION(S) DORSAL WEEK 95 659, 666, 673, 680, 687, 694, 701, 708, 715, 719 C91051 M 89 APPEARANCE THIN 617-618 BEHAVIOR HYPOACTIVE SENSITIVE TO TOUCH 617-618 617-618 EXCRETION NO FECES 617-618 886 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 177 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91051 M 89 SKIN & PELAGE PALE ENTIRE BODY 617-618 SORE/SCAB PAW-HIND-LEFT 547, 554, 561 PAWS-HIND 568, 575, 582, 589, 596, 603, 607, 610, 617-618 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C91052 M 68 APPEARANCE HEAD TILT RIGHT 463, 470 SKIN & PELAGE ROUGH HAIR COAT SORE/SCAB PAW-HIND-LEFT VENTRAL-ABDOMINAL YELLOW HAIR COAT 449, 456, 463, 470 449, 456, 463, 470 211, 218, 225 463, 470 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 232, 316, 323, 407, 414, 22, 29, 36, 43, 50, 127, 134, 141, 148, 239, 246, 253, 260, 330, 337, 344, 351, 421, 428, 435, 442 57, 64, 71, 78, 85, 155, 162, 169, 176, 267, 274, 281, 288, 358, 365, 372, 379, 92, 99, 106 183, 190, 197 295, 302, 309 386, 393, 400 887 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 178 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91053 D 83 APPEARANCE MALOCCLUSION SWOLLEN PAW-HIND-LEFT 498, 505, 547, 554, 561, 568, 575, 580 456, 463, 470 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 512, 519, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 526, 533, 540 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 477, 484, 491 C91054 U 105 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT VENTRAL-ABDOMINAL 680, 687, 694, 701, 708, 715, 722, 729, 732 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 589, 596, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 603, 610, 617, 624, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 631, 638, 645, 652, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 568, 575, 582 659, 666, 673 WART LIKE LESION(S) NOSE WEEK 79 547, 554, 561 888 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 179 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91055 D 82 APPEARANCE HEAD TILT RIGHT MALOCCLUSION 564-566 566, 568-569 BEHAVIOR HYPOACTIVE 569 RECUMBENT STERNAL 569 EXCRETION FEW FECES NO FECES 564 565-566, 568-569 EYES SQUINTED PERI-ORBITAL 569 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA VENTRAL-ABDOMINAL YELLOW HAIR COAT 566, 568-569 566, 568-569 564-566, 568-569 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 889 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 180 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91056 U 105 APPEARANCE MALOCCLUSION SWOLLEN MOUTH 71, 78, 85, 92, 99, 106, 211, 218, 225, 232, 239, 246, 253, 260 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722, 729, 732 56-57, 64 DISCHARGE NASAL RED IN COLOR 56-57, 64 EYES RED DISCHARGE EYES 56-57 RESPIRATION LABORED 56-57 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 113, 120, 127, 134, 141, 148, 155 162, 169, 176, 183, 190, 197, 204 MASS(ES) HEAD WEEK 102 708C, 715C, 722C, 729C, 732C 890 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 181 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91057 D 73 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 510 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C91058 M 83 APPEARANCE MALOCCLUSION 575 SWOLLEN MOUTH 575 SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL 22, 29 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113, 120 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 WART LIKE LESION(S) MOUTH WEEK 83 575 891 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 182 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91059 M 83 APPEARANCE THIN 567-568, 575-576 DISCHARGE GENITAL RED IN COLOR 106, 239, 274 EXCRETION FEW FECES 567-568, 575-576 EYES RED DISCHARGE EYE-LEFT SQUINTED PERI-ORBITAL 232, 239, 246, 253 576 RESPIRATION LABORED 576 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA GENERALIZED RED HAIR COAT HEAD-ENTIRE 568, 575 576 567 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 120, 127, 211, 218, 337, 344, 428, 435, 22, 29, 36, 43, 50, 134, 141, 148, 155, 225, 260, 267, 281, 351, 358, 365, 372, 442, 449, 456, 463, 57, 64, 71, 78, 85, 162, 169, 176, 183, 288, 295, 302, 309, 379, 386, 393, 400, 470, 477, 484, 491, 92, 99, 113 190, 197, 204 316, 323, 330 407, 414, 421 498, 505, 512 892 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 183 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91059 M 83 NORMAL NO REMARKABLE OBSERVATIONS 519, 526, 533, 540, 547, 554, 561 C91060 U 105 EYES DISCOLORED/LIGHT RED EYE-RIGHT RED DISCHARGE EYE-RIGHT 582, 589, 596, 603, 610, 617 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708, 715, 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C91061 D 90 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 603C, 610C, 617C, 624C, 627C 893 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 184 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91062 M 93 APPEARANCE HEAD TILT LEFT HUNCHED THIN 631, 638, 645 638, 645 568, 575, 582, 589, 596, 603, 608, 610-611, 617, 624, 631, 638 645 BEHAVIOR HYPOACTIVE 617, 624, 631, 638, 645 EXCRETION FEW FECES 568, 575, 608, 610-611, 617, 624, 631, 638, 645 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA PALE ENTIRE BODY 638, 645 638, 645 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 894 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 185 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91063 M 100 APPEARANCE THIN 680, 683, 687, 691, 694, 697 EXCRETION FEW FECES 680, 683, 687, 691, 694, 697 EYES PALE EYES 680, 683, 687, 691, 694, 697 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL 680, 683, 687, 691, 694, 697 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C91064 D 97 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 895 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 186 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91064 D 97 NORMAL NO REMARKABLE OBSERVATIONS 659, 666, 673, 679 C91065 U 105 APPEARANCE MALOCCLUSION 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113 C91066 M 86 APPEARANCE HUNCHED THIN 589, 596, 598 547, 554, 561, 568, 575, 582, 589, 596, 598 BEHAVIOR HYPOACTIVE 598 SENSITIVE TO TOUCH 598 EXCRETION FEW FECES 547, 554, 561, 568, 575, 582, 589, 596, 598 SKIN & PELAGE COLD TO TOUCH ENTIRE BODY 598 896 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 187 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91066 M 86 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 C91067 D 100 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 696 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C91068 M 12 APPEARANCE HUNCHED THIN 71, 78 57, 64, 71, 78 DISCHARGE NASAL RED IN COLOR 43, 50 EXCRETION LIQUID FECES NONFORMED FECES 64 71, 78 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36 897 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 188 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91069 D 84 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 585 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C91070 U 105 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT PAW-HIND-RIGHT 729, 732 456, 463 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 491, 498, 582, 589, 673, 680, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 505, 512, 519, 526, 596, 603, 610, 617, 687, 694, 701, 708, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 533, 540, 547, 554, 624, 631, 638, 645, 715, 722 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 470, 477, 484 561, 568, 575 652, 659, 666 C91071 U 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 898 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 189 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91071 U 105 NORMAL NO REMARKABLE OBSERVATIONS 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 C91072 U 105 EXCRETION FEW FECES 728-729, 732 SKIN & PELAGE YELLOW HAIR COAT 728-729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 MASS(ES) MOUTH WEEK 104 728-729, 732 C91073 U 105 APPEARANCE SWOLLEN PAW-HIND-LEFT 673, 680, 687, 694, 701 DISCHARGE GENITAL RED IN COLOR 365, 379, 386 899 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 190 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91073 U 105 EYES RED DISCHARGE EYE-RIGHT 372 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA SORE/SCAB PAW-HIND-LEFT YELLOW HAIR COAT 729, 732 449, 456, 463, 470, 568, 575, 582, 589, 596, 603, 610, 617, 624 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722, 729, 732 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708 715, 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 414, 421, 533, 540, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 428, 435, 442, 477, 547, 554, 561 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 484, 491, 498, 505, 92, 99, 106 183, 190, 197 274, 281, 288 393, 400, 407 512, 519, 526 C91074 M 102 BEHAVIOR SENSITIVE TO TOUCH 711 EXCRETION FEW FECES 708 NO FECES 711 EYES RED DISCHARGE EYE-RIGHT 638, 645 900 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 191 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91074 M 102 EYES RED DISCHARGE EYES 711 SQUINTED PERI-ORBITAL 708, 711 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND YELLOW HAIR COAT 708 344 295 302, 309, 316, 323, 330, 337, 351, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694, 701, 708, 711 687, 694, 701, 708, 711 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 C91075 M 78 BEHAVIOR HYPOACTIVE 540 RECUMBENT STERNAL 540 DISCHARGE GENITAL RED IN COLOR 372 901 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 192 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91075 M 78 EXCRETION FEW FECES 540 EYES RED DISCHARGE EYES 540 RESPIRATION LABORED 540 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 526, 533, 540 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 393, 400, 484, 491, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 407, 414, 421, 428, 498, 505, 512, 519 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 435, 442, 449, 456, 92, 99, 106 183, 190, 197 274, 281, 288 365, 379, 386 463, 470, 477 C91076 U 105 APPEARANCE MALOCCLUSION 43, 50, 57, 64, 71, 148, 155, 162, 169, 239, 246, 253, 260, 330, 337, 344, 351, 421, 428, 435, 442, 512, 519, 526, 533, 603, 610, 617, 624, 694, 701, 708, 715, 78, 85, 92, 99, 106, 113, 120, 127, 134, 141 176, 183, 190, 197, 204, 211, 218, 225, 232 267, 274, 281, 288, 295, 302, 309, 316, 323 358, 365, 372, 379, 386, 393, 400, 407, 414 449, 456, 463, 470, 477, 484, 491, 498, 505 540, 547, 554, 561, 568, 575, 582, 589, 596 631, 638, 645, 652, 659, 666, 673, 680, 687 722, 729, 732 902 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 193 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91076 U 105 DISCHARGE GENITAL RED IN COLOR 491 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT YELLOW HAIR COAT 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687 694, 701, 708, 715, 722, 729, 732 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36 C91077 M 84 APPEARANCE MALOCCLUSION MISSING TEETH SWOLLEN MOUTH 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232, 239 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 588 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 588 547 SKIN & PELAGE COLD TO TOUCH ENTIRE BODY 588 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 903 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 194 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91077 M 84 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148 C91078 D 93 APPEARANCE LIMITED USE LIMB-HIND-RIGHT SWOLLEN PAW-HIND-RIGHT 386, 393, 400, 407, 414, 421, 428 435, 442, 449, 456, 463, 470, 477, 484 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 491, 498, 649 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 505, 512, 519, 526, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 533, 540, 547, 554, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 561, 638, 645 C91079 M 85 APPEARANCE HEAD TILT RIGHT THIN 579, 582, 588-590 568, 575, 579, 582, 588-590 BEHAVIOR ATAXIC HYPOACTIVE 590 579, 582, 588-590 904 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 195 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91079 M 85 EYES RED DISCHARGE EYES 190, 288, 295 RESPIRATION IRREGULAR 568, 575, 579, 582, 588-590 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 211, 218, 316, 323, 407, 414, 498, 505, 22, 29, 36, 43, 50, 127, 134, 141, 148, 225, 232, 239, 246, 330, 337, 344, 351, 421, 428, 435, 442, 512, 519, 526, 533, 57, 64, 71, 78, 85, 155, 162, 169, 176, 253, 260, 267, 274, 358, 365, 372, 379, 449, 456, 463, 470, 540, 547, 554, 561 92, 99, 106 183, 197, 204 281, 302, 309 386, 393, 400 477, 484, 491 C91080 D 98 APPEARANCE THIN 679-681 EXCRETION FEW FECES 679-681 SKIN & PELAGE SORE/SCAB HEAD-ENTIRE PAW-HIND-LEFT 435 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 659, 666, 673, 679-681 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 905 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 196 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91080 D 98 NORMAL NO REMARKABLE OBSERVATIONS 386, 393, 400, 407, 414, 421, 428 WART LIKE LESION(S) EAR-LEFT WEEK 65 VENTRAL-ABDOMINAL WEEK 64 WEEK 76 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 631, 638, 645, 652, 659, 666, 673, 679-681 442, 449 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 638, 645, 652, 659, 666, 673, 679-681 906 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 197 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91043 M6 519 75 TO 526 76 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 40 X 40MM 533 77 TO 561 81 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 60 X 55MM 568 82 TO 582 84 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 65 X 70MM 589 85 TO 590 85 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 80 X 80MM C91056 M6 708 102 TO 722 104 MASS(ES) HEAD WEEK 102 40 X 35MM 729 105 TO 732 105 MASS(ES) HEAD WEEK 102 50 X 40MM C91061 M6 603 87 TO 617 89 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 30 X 30MM 624 90 TO 627 90 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 40 X 50 907 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 198 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M7 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91081 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91082 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91083 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91084 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91085 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91086 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91087 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91088 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91089 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 908 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 199 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M7 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91090 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91091 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91092 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91093 6 8 APPEARANCE MALOCCLUSION 36, 43, 50 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29 C91094 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91095 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91096 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91097 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 909 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 200 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M7 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91098 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91099 D 6 APPEARANCE DEHYDRATED 38-39 BEHAVIOR HYPOACTIVE 38-39 DISCHARGE ORAL RED IN COLOR 38-39 EXCRETION FEW FECES 38-39 SKIN & PELAGE YELLOW HAIR COAT 38-39 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 38 C91100 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 910 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 201 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M7 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91101 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91102 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91103 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91104 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91105 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91106 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91107 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91108 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91109 6 8 APPEARANCE MALOCCLUSION 22, 29, 36, 43, 50 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15 911 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 202 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M7 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91110 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91111 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91112 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91113 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91114 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91115 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91116 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91117 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91118 6 8 SKIN & PELAGE SORE/SCAB HEAD-ENTIRE 15 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 22, 29, 36, 43, 50 912 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 203 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M7 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91119 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91120 6 8 APPEARANCE THIN 50 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL 43, 50 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36 913 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 204 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91121 4 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C91122 4 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C91123 D 98 BEHAVIOR HYPOACTIVE 683 RECUMBENT STERNAL 683 EYES CLOUDY DISCHARGE EYE-RIGHT 113 RESPIRATION LABORED 683 SKIN & PELAGE SORE/SCAB DORSAL 659, 666, 673, 680, 683 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 914 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 205 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91123 D 98 NORMAL NO REMARKABLE OBSERVATIONS 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 575, 582 MASS(ES) AXILLARY REGION-LEFT WEEK 97 AXILLARY REGION-RIGHT WEEK 85 INGUINAL REGION-RIGHT WEEK 94 673, 680, 683 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C 666C, 673C, 680C, 683C 652C, 659C, 666C, 673C, 680C, 683C C91124 4 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C91125 M 96 APPEARANCE MALOCCLUSION 253 THIN 671 EXCRETION FEW FECES NO FECES 659, 666 671 EYES RED DISCHARGE EYES 671 915 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 206 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91125 M 96 RESPIRATION LABORED 671 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 302, 309, 393, 400, 484, 491, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 561, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 568, 575, 582, 589, 92, 99, 106 183, 190, 197 281, 288, 295 372, 379, 386 463, 470, 477 596 MASS(ES) AXILLARY REGION-LEFT WEEK 75 AXILLARY REGION-RIGHT WEEK 76 INGUINAL REGION-RIGHT WEEK 87 519, 526, 533, 540, 547, 554 526, 533, 540, 547, 554 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 671C C91126 2 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 54 C91127 T 105 APPEARANCE MALOCCLUSION 724, 727 EXCRETION FEW FECES 724, 727, 729-730 SKIN & PELAGE SORE/SCAB LATERAL-ABDOMINAL-LEFT 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 722, 724, 727, 729-730 916 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 207 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91127 T 105 SKIN & PELAGE SORE/SCAB LIMB-HIND-LEFT 568, 575, 582, 589, 596, 603 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 610 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 91 631, 638, 645, 652, 659C, 666C, 673C, 680C, 687C, 694C, 701C 708C, 715C, 722C, 724C, 727C, 729-730C C91128 M 75 EXCRETION FEW FECES 523 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 523 VENTRAL-ABDOMINAL 523 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 917 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 208 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91128 M 75 NORMAL NO REMARKABLE OBSERVATIONS 477, 484, 491, 498, 505, 512, 519 C91129 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C91130 D 77 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533-534 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C91131 T 105 APPEARANCE THIN 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694, 701, 708, 715, 722, 729-730 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT PAWS-HIND 687, 694, 701, 708, 715, 722, 729-730 659, 666, 673, 680 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 918 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 209 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91132 M 89 SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL-LEFT 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617-618 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 MASS(ES) INGUINAL REGION-LEFT WEEK 64 442, 449, 456, 463, 470C, 477C, 484C, 491C, 498C, 505C, 512C 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 603C, 610C, 617-618C C91133 4 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C91134 M 75 APPEARANCE HUNCHED THIN 518-519 491, 498, 501, 505, 512, 518-519 EXCRETION FEW FECES 477, 484, 491, 498, 501, 505, 512, 518-519 919 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 210 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91134 M 75 EYES RED DISCHARGE EYES 518-519 SKIN & PELAGE COLD TO TOUCH ENTIRE BODY RED HAIR COAT LIMBS-FRONT 518-519 505, 512, 518-519 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344 MASS(ES) AXILLARY REGION-LEFT WEEK 59 AXILLARY REGION-RIGHT WEEK 56 VENTRAL-ABDOMINAL WEEK 51 407, 414, 421, 428, 435, 442C, 449C, 456C, 463C, 470C, 477C 484C, 491C 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491 351C, 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C 501C, 505C, 512C, 518-519C 920 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 211 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91135 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91136 M 90 APPEARANCE HEAD TILT RIGHT 624 EYES RED DISCHARGE EYE-RIGHT 610, 617, 624 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT YELLOW HAIR COAT 568, 575, 582, 589, 596, 603, 610, 617, 624 624 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C91137 M 103 APPEARANCE LIMITED USE PAW-HIND-RIGHT SWOLLEN PAW-HIND-RIGHT 701, 708, 714-715 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 714-715 921 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 212 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91137 M 103 BEHAVIOR HYPOACTIVE 715 EXCRETION NO FECES 708, 714-715 EYES SQUINTED PERI-ORBITAL 708, 714-715 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA RED HAIR COAT HEAD-ENTIRE 708, 714-715 708, 714-715 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 922 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 213 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91138 T 105 APPEARANCE MALOCCLUSION 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722, 729, 732 EXCRETION NONFORMED FECES 568 SKIN & PELAGE RED HAIR COAT PERINEAL AREA 533 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169 MASS(ES) AXILLARY REGION-LEFT WEEK 99 687C, 694C, 701C, 708C, 715C, 722C, 729C, 732C C91139 M 62 APPEARANCE HEAD TILT RIGHT 432 EYES RED DISCHARGE EYE-RIGHT 421, 428, 432 923 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 214 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91139 M 62 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414 C91140 2 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 54 C91141 D 80 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) LIMB-HIND-RIGHT WEEK 71 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 558C C91142 M 73 BEHAVIOR SENSITIVE TO TOUCH 505 EXCRETION FEW FECES NO FECES 498, 505 505 EYES RED DISCHARGE EYE-LEFT 351 924 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 215 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91142 M 73 EYES RED DISCHARGE EYES 498, 505 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL 505 YELLOW HAIR COAT 505 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 393, 400, 484, 491 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 407, 414, 421, 428, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 358, 365, 435, 442, 449, 456, 92, 99, 106 183, 190, 197 274, 281, 288 372, 379, 386 463, 470, 477 MASS(ES) INGUINAL REGION-RIGHT WEEK 72 498C, 505C C91143 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91144 T 105 APPEARANCE SWOLLEN VENTRAL-ABDOMINAL THIN 729, 731 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722 925 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 216 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91144 T 105 DISCHARGE GENITAL RED IN COLOR 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C91145 2 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 54 C91146 M 75 APPEARANCE HEAD TILT LEFT MALOCCLUSION 509, 512, 519 253, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 509, 512, 519 EYES RED DISCHARGE EYE-LEFT EYES 509, 512, 519 519 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 926 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 217 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91146 M 75 NORMAL NO REMARKABLE OBSERVATIONS 393, 400, 407 MASS(ES) AXILLARY REGION-RIGHT WEEK 71 491C, 498C, 505C, 509C, 512C, 519C C91147 M 77 APPEARANCE HEAD TILT RIGHT THIN 532-533, 535 535 BEHAVIOR HYPOACTIVE SENSITIVE TO TOUCH 532-533 532-533 EXCRETION FEW FECES 532-533, 535 EYES RED DISCHARGE EYES 535 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 407, 414, 498, 505, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 330, 337, 344, 351, 421, 428, 435, 442, 512, 519, 526 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 358, 365, 372, 379, 449, 456, 463, 470, 92, 99, 106 183, 190, 197 274, 281, 288 386, 393, 400 477, 484, 491 927 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 218 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91147 M 77 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 45 309, 316, 323 C91148 T 105 DISCHARGE GENITAL RED IN COLOR 568 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694, 701, 708, 715, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 575 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) AXILLARY REGION-RIGHT WEEK 102 INGUINAL REGION-RIGHT WEEK 98 VENTRAL-ABDOMINAL WEEK 95 VENTRAL-ABDOMINAL-RIGHT WEEK 98 708C, 715C, 722C, 729-730C 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729-730C 659, 666, 673, 680C, 687C, 694C, 701C, 708C, 715C, 722C 729-730C 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729-730C 928 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 219 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91149 M 88 APPEARANCE THIN 610 EYES PALE EYES 610 SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT 610 LIMB-FRONT-LEFT 610 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344 MASS(ES) AXILLARY REGION-LEFT WEEK 82 VENTRAL-ABDOMINAL-RIGHT WEEK 51 568C, 575C, 582C, 589C, 596C, 603C, 610C 351C, 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 582C, 589C, 596C, 603C, 610C 929 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 220 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91150 M 76 EYES PALE EYES 526 RESPIRATION LABORED 526 SKIN & PELAGE BLUE SKIN VENTRAL-CERVICAL 498, 505, 512, 519, 526 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421 MASS(ES) VENTRAL-CERVICAL WEEK 62 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C 505C, 512C, 519C, 526C C91151 M 74 APPEARANCE HEAD TILT LEFT THIN OTHER 512 491, 498, 505, 512 498C EXCRETION FEW FECES 484, 491, 498, 505, 512 930 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 221 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91151 M 74 EYES RED DISCHARGE EYES 512 HOUSING,FOOD,WATER & MAINT. WATER BOTTLE PLACED ON CAGE 491 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 442, 449, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 379, 386, 456, 463, 470, 477 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 393, 400, 407, 414, 92, 99, 106 183, 190, 197 274, 281, 288 421, 428, 435 MASS(ES) AXILLARY REGION-RIGHT WEEK 47 323C, 330C, 337, 344, 351, 358, 365, 372 C91152 4 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C91153 M 81 SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB AXILLARY REGION-RIGHT 561 554, 561 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 931 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 222 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91153 M 81 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C C91154 D 78 EXCRETION NONFORMED FECES 204 EYES RED DISCHARGE EYE-RIGHT 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533, 540-541 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 932 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 223 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91155 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C91156 M 75 APPEARANCE MALOCCLUSION 169, 176, 225 DISCHARGE NASAL RED IN COLOR 169 EXCRETION FEW FECES 519 EYES PALE EYES 519 SKIN & PELAGE RED HAIR COAT PERINEAL AREA SORE/SCAB PAW-HIND-RIGHT PAWS-HIND 512, 519 463, 470, 477, 484, 491, 498, 505, 512, 519 519 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 218, 232, 316, 323, 449, 456 22, 29, 36, 43, 50, 127, 134, 141, 148, 239, 246, 253, 260, 330, 337, 344, 351, 57, 64, 71, 78, 85, 155, 162, 183, 190, 267, 274, 281, 288, 358, 365, 372, 379, 92, 99, 106 197, 204, 211 295, 302, 309 428, 435, 442 933 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 224 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91156 M 75 MASS(ES) AXILLARY REGION-LEFT WEEK 56 386, 393, 400, 407, 414, 421 C91157 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 731 C91158 M 94 DISCHARGE GENITAL RED IN COLOR 400 SKIN & PELAGE SORE/SCAB LIMBS-HIND PAWS-HIND 596, 603, 610 617, 624, 631, 638, 645, 652 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 407, 414, 421, 428, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 435, 442, 449, 456, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 463 934 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 225 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91158 M 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C 624C, 631C, 638C, 645C, 652C C91159 T 105 EYES CLEAR DISCHARGE EYE-LEFT 659, 666 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) AXILLARY REGION-LEFT WEEK 85 589, 596, 603, 610, 617, 624, 631C, 638C, 645C, 652C, 659C, 666C 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729-730C C91160 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 935 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 226 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91161 M 61 APPEARANCE HEAD TILT RIGHT THIN 421 414, 421 EXCRETION FEW FECES 400, 414, 421 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 393, 400, 407, 414, 421 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 183, 190, 197, 204, 211, 218, 225, 232, 239 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330 337, 344, 351, 358, 365, 372, 379, 386 WART LIKE LESION(S) MOUTH WEEK 21 141, 148, 155, 162, 169, 176 C91162 2 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 54 936 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 227 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91163 2 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 54 C91164 M 95 APPEARANCE HUNCHED 659 EXCRETION FEW FECES 659 EYES RED DISCHARGE EYES 652, 659 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT 631, 638, 645, 652, 659 652, 659 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 86 596, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C 937 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 228 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91165 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91166 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 673, 680, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 610, 617, 687, 694, 701, 708, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 624, 631, 638, 645, 715, 722, 729, 731 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 652, 659, 666 MASS(ES) HEAD WEEK 86 596, 603 C91167 D 103 APPEARANCE LIMITED USE PAW-HIND-LEFT SWOLLEN PAW-HIND-LEFT 701, 708, 715, 717 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 717 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456 938 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 229 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91167 D 103 MASS(ES) DORSAL WEEK 82 568, 575, 582, 589, 596, 603C, 610C, 617C, 624C, 631C, 638C 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 717C C91168 M 84 APPEARANCE HEAD TILT RIGHT THIN 588 582, 588 BEHAVIOR SENSITIVE TO TOUCH 579, 582, 588 EXCRETION FEW FECES 582 NO FECES 588 SKIN & PELAGE YELLOW HAIR COAT 579, 582, 588 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 939 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 230 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91169 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91170 M 89 APPEARANCE HEAD TILT RIGHT HUNCHED MALOCCLUSION SWOLLEN MOUTH THIN 603, 610, 617-618 617-618 540, 547, 551, 554, 561, 568, 575, 582, 589, 596, 603, 610 617-618 551, 554, 561 603, 610, 617-618 EXCRETION FEW FECES 551, 554, 561, 596, 603, 610, 617-618 SKIN & PELAGE PALE ENTIRE BODY 617-618 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 533 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) MOUTH WEEK 71 491, 498, 505, 512, 519, 526 940 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 231 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91171 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91172 T 105 APPEARANCE MALOCCLUSION MISSING TEETH 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708, 715, 722, 729-730 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729-730 SKIN & PELAGE SORE/SCAB MOUTH PAWS-HIND YELLOW HAIR COAT 582 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 722, 729-730 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 603, 610, 617 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 603, 610 941 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 232 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91173 M 58 APPEARANCE THIN 393, 400, 404 BEHAVIOR HYPOACTIVE 404 DISCHARGE NASAL RED IN COLOR 393, 400, 404 EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL 393, 404 400, 404 SKIN & PELAGE YELLOW HAIR COAT 400, 404 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 942 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 233 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91174 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C91175 M 86 APPEARANCE HEAD TILT LEFT 596, 600 BEHAVIOR ATAXIC 600 EYES RED DISCHARGE EYE-LEFT 600 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) AXILLARY REGION-LEFT WEEK 74 512, 519, 526, 533, 540, 547, 554, 561C, 568C, 575C, 582C, 589C 596C, 600C 943 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 234 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91176 T 105 APPEARANCE LIMITED USE PAW-HIND-LEFT SWOLLEN PAW-HIND-LEFT 701, 708, 715, 722, 729-730 674, 680, 687, 694, 701, 708, 715, 722, 729-730 SKIN & PELAGE SORE/SCAB LIMB-HIND-RIGHT 589 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 666, 673 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 596, 603, 610, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 617, 624, 631, 638, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 645, 652, 659 C91177 M 94 APPEARANCE THIN 582, 589, 596, 603, 610, 617, 624, 631, 638, 640, 645, 652 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 944 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 235 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91177 M 94 MASS(ES) AXILLARY REGION-LEFT WEEK 63 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 640C, 645C, 652C C91178 M 91 APPEARANCE HEAD TILT LEFT MALOCCLUSION 631 372, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 BEHAVIOR ATAXIC 631 HYPOACTIVE 631 EXCRETION FEW FECES 617, 624, 631 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 393, 400, 526, 533, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 407, 414, 421, 428, 540, 547, 554, 561 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 435, 442, 491, 498, 92, 99, 106 183, 190, 197 274, 281, 288 365, 379, 386 505, 512, 519 MASS(ES) AXILLARY REGION-LEFT WEEK 65 AXILLARY REGION-RIGHT WEEK 67 449C, 456C, 463C, 470C, 477C, 484C 463, 470, 477, 484 945 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 236 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91178 M 91 MASS(ES) VENTRAL-ABDOMINAL WEEK 90 624C, 631C WART LIKE LESION(S) HEAD-MAXILLARY-LEFT WEEK 89 617 C91179 M 47 APPEARANCE THIN 324 SKIN & PELAGE COLD TO TOUCH ENTIRE BODY PALE ENTIRE BODY SORE/SCAB VENTRAL-CERVICAL 323 323-324 323-324 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 VENTRAL-ABDOMINAL-RIGHT WEEK 36 260, 267, 274C, 281C, 288C, 295C, 302C, 309C, 316C, 323-324C 246C, 253C, 260C, 267C, 274C, 281C, 288C, 295C, 302C, 309C, 316C 323-324C 946 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 237 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91180 D 64 SKIN & PELAGE SORE/SCAB DORSAL-CERVICAL-LEFT 15, 22 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113, 120 127, 134, 141, 148, 155, 162, 190, 197, 204, 211 MASS(ES) AXILLARY REGION-LEFT WEEK 26 AXILLARY REGION-RIGHT WEEK 38 LIMB-HIND-LEFT WEEK 25 VENTRAL-ABDOMINAL-RIGHT WEEK 32 176, 183 260, 267, 274, 281, 288 169 218C, 225C, 232C, 239C, 246C, 253C, 260C, 267C, 274C, 281C, 288C 295C, 302C, 309C, 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C 446C C91181 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C91182 M 88 APPEARANCE THIN 589, 596, 603, 610 BEHAVIOR HYPOACTIVE 440, 610 EXCRETION FEW FECES 440, 589, 596, 603, 610 947 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 238 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91182 M 88 EYES RED DISCHARGE EYES 440, 561, 568, 575, 582, 589, 596, 603, 610 RESPIRATION LABORED 440 SKIN & PELAGE YELLOW HAIR COAT 582, 589, 596, 603, 610 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554 MASS(ES) INGUINAL REGION-LEFT WEEK 87 603C, 610C C91183 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 512, 519, 603, 610, 694, 701, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 127, 134, 141, 148, 155, 162, 169, 176, 218, 225, 232, 239, 246, 253, 260, 267, 309, 316, 323, 330, 337, 344, 351, 358, 435, 442, 449, 456, 463, 470, 477, 484, 526, 533, 540, 547, 554, 561, 568, 575, 617, 624, 631, 638, 645, 652, 659, 666, 708, 715, 722, 729-730 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 491, 498, 505 582, 589, 596 673, 680, 687 948 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 239 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91183 T 105 MASS(ES) AXILLARY REGION-LEFT WEEK 58 400, 407, 414, 421, 428 C91184 M 90 APPEARANCE SWOLLEN VENTRAL-ABDOMINAL 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 BEHAVIOR RECUMBENT STERNAL 629 EXCRETION FEW FECES NO FECES 617, 624 629 EYES RED DISCHARGE EYES 629 RESPIRATION LABORED 629 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB PAW-HIND-LEFT YELLOW HAIR COAT 456, 463, 470, 477, 484 582, 589, 596, 603, 610, 617, 624, 629 624, 629 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 949 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 240 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91184 M 90 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 491, 498, 505 C91185 M 67 APPEARANCE HUNCHED THIN 442, 449, 456, 463-464 449, 456, 463-464 EXCRETION FEW FECES 464 EYES RED DISCHARGE EYES 442, 449, 456, 463-464 SKIN & PELAGE PALE ENTIRE BODY 456, 463-464 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 950 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 241 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91186 M 98 APPEARANCE THIN 631, 638, 645, 652, 659, 666, 673, 680 SKIN & PELAGE PALE ENTIRE BODY 680 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) INGUINAL REGION-LEFT WEEK 76 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C C91187 M 73 APPEARANCE HEAD TILT LEFT HUNCHED MALOCCLUSION THIN 508-509 491, 498, 501, 505, 508-509 435, 442, 449, 463 491, 498, 501, 505, 508-509 BEHAVIOR ATAXIC HYPOACTIVE 508-509 509 951 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 242 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91187 M 73 EXCRETION FEW FECES 498, 501, 505, 508 NO FECES 509 NONFORMED FECES 197, 491 EYES RED DISCHARGE EYE-RIGHT 508-509 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 204 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 428, 456, 470, 477, 484 MASS(ES) AXILLARY REGION-LEFT WEEK 71 491C, 498C, 501C, 505C, 508-509C C91188 T 105 APPEARANCE SWOLLEN INGUINAL REGION-LEFT INGUINAL REGION-RIGHT 631, 638, 645, 652 631, 638, 645, 652 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 582, 589, 596, 603, 610 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 952 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 243 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91188 T 105 NORMAL NO REMARKABLE OBSERVATIONS 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505 MASS(ES) AXILLARY REGION-LEFT WEEK 74 VENTRAL-CERVICAL-LEFT WEEK 88 VENTRAL-THORACIC-LEFT WEEK 101 512, 519, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 731C 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C 687C, 694C, 701C, 708C, 715C, 722C, 729C, 731C 701C, 708C, 715C, 722C, 729C, 731C C91189 M 93 EXCRETION FEW FECES 645 SKIN & PELAGE SORE/SCAB AXILLARY REGION-RIGHT LIMBS-HIND PAW-HIND-LEFT 645 603, 610, 617, 624, 631, 638, 645 554, 561, 568, 575, 582, 589, 596 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 953 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 244 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91189 M 93 NORMAL NO REMARKABLE OBSERVATIONS 477, 484, 491, 498, 505, 512, 519 MASS(ES) AXILLARY REGION-RIGHT WEEK 88 INGUINAL REGION-RIGHT WEEK 76 610C, 617C, 624C, 631C, 638C, 645C 526, 533, 540, 547C, 554C, 561C, 568, 575, 582, 589, 596 C91190 T 105 APPEARANCE MALOCCLUSION 589, 596, 603, 610, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 617, 624, 631, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 638 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) VENTRAL-CERVICAL WEEK 100 694C, 701C, 708C, 715C, 722C, 729C, 731C 954 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 245 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91123 F1 589 85 MASS(ES) AXILLARY REGION-RIGHT WEEK 85 20 X 20MM 596 86 MASS(ES) AXILLARY REGION-RIGHT WEEK 85 30 X 25MM 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 85 35 X 30MM 610 88 TO 645 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 85 35 X 40MM 652 94 TO 666 96 MASS(ES) AXILLARY REGION-RIGHT WEEK 85 40 X 50MM 673 97 TO 683 98 MASS(ES) AXILLARY REGION-RIGHT WEEK 85 50 X 60MM 652 94 MASS(ES) INGUINAL REGION-RIGHT WEEK 94 15 X 15MM 659 95 MASS(ES) INGUINAL REGION-RIGHT WEEK 94 20 X 30MM 955 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 246 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91123 F1 666 96 MASS(ES) INGUINAL REGION-RIGHT WEEK 94 30 X 30MM 673 97 TO 683 98 MASS(ES) INGUINAL REGION-RIGHT WEEK 94 40 X 40MM C91125 F1 603 87 TO 624 90 MASS(ES) INGUINAL REGION-RIGHT WEEK 87 35 X 35MM 631 91 TO 645 93 MASS(ES) INGUINAL REGION-RIGHT WEEK 87 45 X 35MM 652 94 MASS(ES) INGUINAL REGION-RIGHT WEEK 87 80 X 40MM 659 95 TO 666 96 MASS(ES) INGUINAL REGION-RIGHT WEEK 87 60 X 40MM 671 96 TO 671 96 MASS(ES) INGUINAL REGION-RIGHT WEEK 87 70 X 55MM C91127 F1 659 95 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 91 25 X 30MM 956 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 247 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91127 F1 666 96 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 91 30 X 30MM 673 97 TO 701 101 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 91 40 X 40MM 708 102 TO 730 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 91 60 X 50MM C91132 F1 470 68 TO 484 70 MASS(ES) INGUINAL REGION-LEFT WEEK 64 15 X 15MM 491 71 MASS(ES) INGUINAL REGION-LEFT WEEK 64 30 X 30MM 498 72 TO 561 81 MASS(ES) INGUINAL REGION-LEFT WEEK 64 70 X 50MM 568 82 TO 582 84 MASS(ES) INGUINAL REGION-LEFT WEEK 64 80 X 65MM 589 85 MASS(ES) INGUINAL REGION-LEFT WEEK 64 90 X 80MM 957 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 248 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91132 F1 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 64 100 X 90MM 603 87 MASS(ES) INGUINAL REGION-LEFT WEEK 64 110 X 90MM 610 88 MASS(ES) INGUINAL REGION-LEFT WEEK 64 110 X 120MM 617 89 TO 618 89 MASS(ES) INGUINAL REGION-LEFT WEEK 64 80 X 90MM C91134 F1 442 64 TO 491 71 MASS(ES) AXILLARY REGION-LEFT WEEK 59 10 X 10MM 351 51 TO 407 59 MASS(ES) VENTRAL-ABDOMINAL WEEK 51 10 X 10MM 414 60 TO 421 61 MASS(ES) VENTRAL-ABDOMINAL WEEK 51 20 X 20MM 428 62 TO 442 64 MASS(ES) VENTRAL-ABDOMINAL WEEK 51 30 X 30MM 958 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 249 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91134 F1 449 65 TO 463 67 MASS(ES) VENTRAL-ABDOMINAL WEEK 51 35 X 40MM 470 68 TO 519 75 MASS(ES) VENTRAL-ABDOMINAL WEEK 51 60 X 40MM C91138 F1 687 99 TO 694 100 MASS(ES) AXILLARY REGION-LEFT WEEK 99 50 X 40MM 701 101 MASS(ES) AXILLARY REGION-LEFT WEEK 99 60 X 50MM 708 102 TO 732 105 MASS(ES) AXILLARY REGION-LEFT WEEK 99 50 X 70MM C91141 F1 491 71 TO 519 75 MASS(ES) LIMB-HIND-RIGHT WEEK 71 15 X 15MM 526 76 TO 558 80 MASS(ES) LIMB-HIND-RIGHT WEEK 71 20 X 30MM C91142 F1 498 72 TO 505 73 MASS(ES) INGUINAL REGION-RIGHT WEEK 72 15 X 15MM 959 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 250 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91146 F1 491 71 TO 519 75 MASS(ES) AXILLARY REGION-RIGHT WEEK 71 10 X 15MM C91148 F1 708 102 TO 730 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 102 50 X 45MM 680 98 TO 687 99 MASS(ES) INGUINAL REGION-RIGHT WEEK 98 40 X 30MM 694 100 TO 730 105 MASS(ES) INGUINAL REGION-RIGHT WEEK 98 40 X 50MM 680 98 TO 701 101 MASS(ES) VENTRAL-ABDOMINAL WEEK 95 20 X 20MM 708 102 TO 730 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 95 20 X 20MM 680 98 TO 701 101 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 98 20 X 20MM 708 102 TO 730 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 98 30 X 25MM 960 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 251 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91149 F1 568 82 TO 582 84 MASS(ES) AXILLARY REGION-LEFT WEEK 82 20 X 20MM 589 85 MASS(ES) AXILLARY REGION-LEFT WEEK 82 25 X 25MM 596 86 TO 610 88 MASS(ES) AXILLARY REGION-LEFT WEEK 82 35 X 30MM 351 51 TO 365 53 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 51 20 X 10MM 372 54 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 51 30 X 20MM 379 55 TO 386 56 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 51 35 X 25MM 393 57 TO 407 59 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 51 35 X 30MM 414 60 TO 421 61 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 51 40X35MM 961 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 252 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91149 F1 428 62 TO 435 63 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 51 40 X 40MM 442 64 TO 561 81 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 51 45 X 40MM 568 82 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 51 50 X 50MM 603 87 TO 610 88 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 51 50 X 55MM C91150 F1 428 62 TO 435 63 MASS(ES) VENTRAL-CERVICAL WEEK 62 30 X 30MM 442 64 MASS(ES) VENTRAL-CERVICAL WEEK 62 35 X 35MM 449 65 TO 463 67 MASS(ES) VENTRAL-CERVICAL WEEK 62 40 X 40MM 470 68 TO 491 71 MASS(ES) VENTRAL-CERVICAL WEEK 62 45 X 45MM 962 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 253 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91150 F1 498 72 TO 526 76 MASS(ES) VENTRAL-CERVICAL WEEK 62 45 X 60MM C91151 F1 498 72 APPEARANCE OTHER GENITAL DISCHARGE, GREEN 323 47 TO 330 48 MASS(ES) AXILLARY REGION-RIGHT WEEK 47 10 X 10MM C91153 F1 414 60 TO 484 70 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 10 X 10MM 491 71 TO 533 77 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 20 X 20MM 540 78 TO 561 81 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 40 X 25MM C91158 F1 470 68 TO 484 70 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 10 X 10MM 491 71 TO 533 77 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 20 X 20MM 963 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 254 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91158 F1 540 78 TO 561 81 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 35 X 30MM 568 82 TO 582 84 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 45 X 50MM 589 85 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 50 X 55MM 596 86 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 60 X 55MM 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 65 X 60MM 610 88 TO 624 90 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 90 X 75MM 631 91 TO 645 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 100 X 80MM 652 94 TO 652 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 110 X 90MM 964 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 255 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91158 F1 652 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 110 X 90MM C91159 F1 631 91 TO 652 94 MASS(ES) AXILLARY REGION-LEFT WEEK 85 10 X 10MM 659 95 TO 701 101 MASS(ES) AXILLARY REGION-LEFT WEEK 85 15 X 15MM 708 102 TO 730 105 MASS(ES) AXILLARY REGION-LEFT WEEK 85 20 X 20MM C91164 F1 603 87 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 86 20 X 20MM 610 88 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 86 20 X 25MM C91167 F1 603 87 MASS(ES) DORSAL WEEK 82 15 X 20MM 610 88 MASS(ES) DORSAL WEEK 82 20 X 20MM 965 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 256 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91167 F1 617 89 MASS(ES) DORSAL WEEK 82 20 X 20MM 624 90 MASS(ES) DORSAL WEEK 82 20 X 20MM 631 91 MASS(ES) DORSAL WEEK 82 20 X 20MM 638 92 MASS(ES) DORSAL WEEK 82 20 X 20MM 645 93 MASS(ES) DORSAL WEEK 82 20 X 20MM 652 94 MASS(ES) DORSAL WEEK 82 20 X 30MM 659 95 MASS(ES) DORSAL WEEK 82 20 X 30MM 666 96 MASS(ES) DORSAL WEEK 82 20 X 30MM 966 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 257 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91167 F1 673 97 MASS(ES) DORSAL WEEK 82 20 X 30MM 680 98 MASS(ES) DORSAL WEEK 82 35 X 45MM 687 99 MASS(ES) DORSAL WEEK 82 40 X 45MM 694 100 MASS(ES) DORSAL WEEK 82 40 X 45MM 701 101 MASS(ES) DORSAL WEEK 82 40 X 45MM 708 102 MASS(ES) DORSAL WEEK 82 40 X 45MM 715 103 MASS(ES) DORSAL WEEK 82 40 X 45MM 717 103 MASS(ES) DORSAL WEEK 82 40 X 45MM 967 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 258 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91175 F1 561 81 TO 582 84 MASS(ES) AXILLARY REGION-LEFT WEEK 74 35 X 30MM 589 85 MASS(ES) AXILLARY REGION-LEFT WEEK 74 45 X 40MM 596 86 TO 600 86 MASS(ES) AXILLARY REGION-LEFT WEEK 74 50 X 45MM C91177 F1 435 63 MASS(ES) AXILLARY REGION-LEFT WEEK 63 30MM X 40MM 442 64 MASS(ES) AXILLARY REGION-LEFT WEEK 63 50 X 45MM 449 65 TO 463 67 MASS(ES) AXILLARY REGION-LEFT WEEK 63 60 X 65MM 470 68 TO 491 71 MASS(ES) AXILLARY REGION-LEFT WEEK 63 65 X 70MM 498 72 TO 519 75 MASS(ES) AXILLARY REGION-LEFT WEEK 63 80 X 70MM 968 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 259 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91177 F1 526 76 TO 540 78 MASS(ES) AXILLARY REGION-LEFT WEEK 63 90 X 70MM 547 79 TO 561 81 MASS(ES) AXILLARY REGION-LEFT WEEK 63 90 X 80MM 568 82 TO 589 85 MASS(ES) AXILLARY REGION-LEFT WEEK 63 90 X 100MM 596 86 TO 603 87 MASS(ES) AXILLARY REGION-LEFT WEEK 63 100 X 110MM 610 88 TO 652 94 MASS(ES) AXILLARY REGION-LEFT WEEK 63 130 X 110MM C91178 F1 449 65 TO 484 70 MASS(ES) AXILLARY REGION-LEFT WEEK 65 10 X 10MM 624 90 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL WEEK 90 10 X 10MM C91179 F1 274 40 TO 288 42 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 10 X 10MM 969 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 260 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91179 F1 295 43 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 20 X 20MM 302 44 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 30 X 30MM 309 45 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 45 X 55MM 316 46 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 65 X 60MM 323 47 TO 324 47 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 80 X 65MM 246 36 TO 267 39 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 36 25 X 20MM 274 40 TO 295 43 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 36 30 X 25MM 302 44 TO 309 45 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 36 50 X 40MM 970 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 261 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91179 F1 316 46 TO 324 47 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 36 65 X 50MM C91180 F1 218 32 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 15 X 15MM 225 33 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 20 X 30MM 232 34 TO 239 35 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 30 X 30MM 246 36 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 45 X 40MM 253 37 TO 260 38 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 50 X 55MM 267 39 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 60 X 55MM 274 40 TO 302 44 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 70 X 65MM 971 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 262 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91180 F1 309 45 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 80 X 80MM 316 46 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 90 X 80MM 323 47 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 110 X 90MM 330 48 TO 344 50 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 110 X 95MM 351 51 TO 421 61 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 130 X 110MM 428 62 TO 435 63 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 145 X 115MM 442 64 TO 446 64 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 150 X 120MM C91182 F1 603 87 TO 610 88 MASS(ES) INGUINAL REGION-LEFT WEEK 87 10 X 15MM 972 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 263 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91186 F1 526 76 TO 561 81 MASS(ES) INGUINAL REGION-LEFT WEEK 76 20 X 20MM 568 82 TO 575 83 MASS(ES) INGUINAL REGION-LEFT WEEK 76 30 X 30MM 582 84 TO 589 85 MASS(ES) INGUINAL REGION-LEFT WEEK 76 50 X 40MM 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 76 60 X 55MM 603 87 MASS(ES) INGUINAL REGION-LEFT WEEK 76 80 X 60MM 610 88 TO 652 94 MASS(ES) INGUINAL REGION-LEFT WEEK 76 80 X 75MM 659 95 TO 673 97 MASS(ES) INGUINAL REGION-LEFT WEEK 76 80 X 80MM 680 98 TO 680 98 MASS(ES) INGUINAL REGION-LEFT WEEK 76 90 X 80MM 973 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 264 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91186 F1 680 98 MASS(ES) INGUINAL REGION-LEFT WEEK 76 90 X 80MM C91187 F1 491 71 TO 501 72 MASS(ES) AXILLARY REGION-LEFT WEEK 71 15 X 15MM 505 73 MASS(ES) AXILLARY REGION-LEFT WEEK 71 20 X 20MM 508 73 TO 509 73 MASS(ES) AXILLARY REGION-LEFT WEEK 71 25 X 25MM C91188 F1 526 76 TO 561 81 MASS(ES) AXILLARY REGION-LEFT WEEK 74 20 X 15MM 568 82 MASS(ES) AXILLARY REGION-LEFT WEEK 74 20 X 20MM 575 83 TO 589 85 MASS(ES) AXILLARY REGION-LEFT WEEK 74 30 X 30MM 596 86 MASS(ES) AXILLARY REGION-LEFT WEEK 74 35 X 35MM 974 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 265 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91188 F1 603 87 TO 638 92 MASS(ES) AXILLARY REGION-LEFT WEEK 74 40 X 40MM 645 93 TO 652 94 MASS(ES) AXILLARY REGION-LEFT WEEK 74 45 X 45MM 659 95 TO 666 96 MASS(ES) AXILLARY REGION-LEFT WEEK 74 45 X 55MM 673 97 TO 680 98 MASS(ES) AXILLARY REGION-LEFT WEEK 74 60 X 55MM 687 99 TO 701 101 MASS(ES) AXILLARY REGION-LEFT WEEK 74 70 X 55MM 708 102 TO 722 104 MASS(ES) AXILLARY REGION-LEFT WEEK 74 30 X 30MM 729 105 MASS(ES) AXILLARY REGION-LEFT WEEK 74 10 X 10MM 731 105 MASS(ES) AXILLARY REGION-LEFT WEEK 74 10 X 10MM 975 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 266 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91188 F1 610 88 TO 638 92 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 88 30 X 30MM 645 93 TO 666 96 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 88 45 X 45MM 673 97 TO 701 101 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 88 45 X 60MM 708 102 TO 731 105 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 88 65 X 70MM 701 101 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 101 10 X 10MM 708 102 TO 731 105 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 101 60 X 55MM C91189 F1 610 88 MASS(ES) AXILLARY REGION-RIGHT WEEK 88 20 X 20MM 617 89 TO 645 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 88 30 X 30MM 976 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 267 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91189 F1 547 79 TO 561 81 MASS(ES) INGUINAL REGION-RIGHT WEEK 76 15 X 15MM C91190 F1 694 100 TO 701 101 MASS(ES) VENTRAL-CERVICAL WEEK 100 25 X 25MM 708 102 TO 731 105 MASS(ES) VENTRAL-CERVICAL WEEK 100 50 X 45MM 977 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 268 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91191 D 98 APPEARANCE MALOCCLUSION SWOLLEN LIMB-HIND-LEFT MOUTH PAW-FRONT-LEFT PAW-HIND-RIGHT 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 582 435, 442, 449 407, 414, 421, 428 SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB PAW-HIND-LEFT PAWS-HIND VENTRAL-CERVICAL VENTRAL-CERVICAL-LEFT 344, 351, 358 582, 589, 596, 603, 610 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 29 274, 281, 288, 295, 302, 309, 316, 323, 330, 337 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 239, 246, 253, 260, 267 MASS(ES) AXILLARY REGION-LEFT WEEK 92 INGUINAL REGION-RIGHT WEEK 76 WEEK 83 638C, 645C, 652C, 659C, 666C, 673C, 680C 526C, 533C 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652C, 659C, 666C, 673C, 680C 978 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 269 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91191 D 98 MASS(ES) MOUTH WEEK 85 589 C91192 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C91193 M 91 APPEARANCE HEAD TILT RIGHT 629 BEHAVIOR CIRCLING RIGHT 629, 631 DISCHARGE NASAL RED IN COLOR 631 EXCRETION FEW FECES NO FECES 617, 624, 631 629 RESPIRATION IRREGULAR 629 SKIN & PELAGE RED HAIR COAT PERINEAL AREA SORE/SCAB DORSAL-CERVICAL PAW-HIND-RIGHT 631 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 629, 631 979 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 270 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91193 M 91 SKIN & PELAGE SORE/SCAB PAWS-FRONT 29, 36, 43, 50, 57, 64 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 148, 155, 239, 246, 330, 337, 421, 428, 512, 519 22, 71, 78, 85, 92, 162, 169, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 99, 106, 113, 120, 127, 134, 141 190, 197, 204, 211, 218, 225, 232 281, 288, 295, 302, 309, 316, 323 372, 379, 386, 393, 400, 407, 414 463, 470, 477, 484, 491, 498, 505 MASS(ES) AXILLARY REGION-LEFT WEEK 85 589, 596C, 603C, 610C, 617C, 624C, 629C, 631C C91194 M 104 APPEARANCE MALOCCLUSION THIN 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 673 680, 687, 694, 701-702, 708, 715, 722, 728 673, 680, 687, 694, 701-702, 708, 715, 722, 728 BEHAVIOR RECUMBENT STERNAL 728 EXCRETION FEW FECES 702, 708, 715, 722, 728 980 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 271 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91194 M 104 EYES RED DISCHARGE EYE-LEFT 204 RESPIRATION LABORED 728 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 MASS(ES) AXILLARY REGION-RIGHT WEEK 71 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701-702C, 708C 715C, 722C, 728C C91195 M 100 APPEARANCE HEAD TILT RIGHT THIN 659, 666, 673, 676, 680, 683, 687, 691, 694 673, 676, 680, 683, 687, 691, 694 BEHAVIOR HYPOACTIVE 691, 694 EXCRETION FEW FECES 673, 676, 680, 683, 687, 691, 694 981 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 272 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91195 M 100 EYES RED DISCHARGE EYE-RIGHT 610, 617 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 676C, 680C, 683C, 687C, 691C, 694C C91196 M 83 APPEARANCE THIN 579 BEHAVIOR ATAXIC 579 HYPOACTIVE 579 EXCRETION FEW FECES NO FECES 565, 575 568, 579 EYES PALE EYES 565, 568 982 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 273 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91196 M 83 EYES RED DISCHARGE EYES 575, 579 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL PALE EARS RED HAIR COAT HEAD-ENTIRE SORE/SCAB PAW-HIND-LEFT 565, 568, 575, 579 579 565, 568, 575, 579 579 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547 MASS(ES) INGUINAL REGION-RIGHT WEEK 80 554C, 561C, 565C, 568C, 575C, 579C 983 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 274 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91197 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729-730 C91198 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91199 M 97 APPEARANCE HEAD TILT RIGHT 673 BEHAVIOR ATAXIC 673 CIRCLING LEFT 673 EXCRETION FEW FECES 652, 659, 666, 673 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT 652 652, 659, 666, 673 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 984 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 275 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91199 M 97 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645 C91200 M 87 BEHAVIOR SENSITIVE TO TOUCH 568, 575, 582, 589, 596, 603, 607 SKIN & PELAGE SORE/SCAB HEAD-CRANIAL SHOULDER-RIGHT 603, 607 29 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 120, 127, 211, 218, 302, 309, 393, 400, 484, 491, 22, 36, 43, 50, 57, 134, 141, 148, 155, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 64, 71, 78, 85, 92, 162, 169, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 99, 106, 113 190, 197, 204 281, 288, 295 372, 379, 386 463, 470, 477 554, 561 MASS(ES) HEAD WEEK 82 568, 575C, 582C, 589C, 596C, 603C, 607C 985 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 276 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91201 T 105 EYES RED DISCHARGE EYES 715, 722, 729-730 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 673, 680, 687, 694, 701, 708, 715, 722 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 435, 442, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 449, 456, 463, 470, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 386, 393, 400, 407, 477, 484, 491, 498, 92, 99, 106 183, 190, 197 274, 281, 288 414, 421, 428 505 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 INGUINAL REGION-RIGHT WEEK 97 VENTRAL-ABDOMINAL-RIGHT WEEK 49 WEEK 105 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729-730C 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729-730C 337C, 344C, 351C, 358, 365, 372, 379 729-730C WART LIKE LESION(S) MOUTH WEEK 52 358, 365 986 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 277 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91202 M 85 SKIN & PELAGE BLACK SKIN VENTRAL-THORACIC-RIGHT 582, 589-590 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 INGUINAL REGION-LEFT WEEK 84 VENTRAL-THORACIC-RIGHT WEEK 84 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589-590C 582C, 589-590C 582C, 589-590C C91203 M 76 BEHAVIOR RECUMBENT LATERAL 532 DISCHARGE NASAL RED IN COLOR 526 EXCRETION FEW FECES 526 987 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 278 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91203 M 76 EYES SQUINTED PERI-ORBITAL 526, 532 RESPIRATION IRREGULAR 532 SKIN & PELAGE PALE ENTIRE BODY 532 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 414, 421, 428, 435, 442, 449C, 456C, 463, 470C, 477C, 484C, 491C 498C, 505C, 512C, 519C, 526C, 532C C91204 D 61 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 426 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232 988 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 279 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91204 D 61 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 239C, 246C, 253C, 260C, 267C, 274C, 281C, 288C, 295C, 302C, 309C 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C 393C, 400C, 407C, 414C, 421C, 426C C91205 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91206 M 75 APPEARANCE MALOCCLUSION 491, 498, 505, 512-513, 519 BEHAVIOR HYPOACTIVE 512-513, 519 EYES PALE EYES 519 RESPIRATION AUDIBLE 489, 491 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA RED HAIR COAT HEAD-ENTIRE 513, 519 489, 491, 498, 505, 512 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 989 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 280 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91206 M 75 NORMAL NO REMARKABLE OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484 C91207 T 105 APPEARANCE MALOCCLUSION 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 DISCHARGE NASAL RED IN COLOR 386 EYES RED DISCHARGE EYES 386 SKIN & PELAGE PALE ENTIRE BODY 617 YELLOW HAIR COAT 645 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 990 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 281 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91208 M 75 APPEARANCE HUNCHED THIN 518-519 498, 505, 512, 518-519 EXCRETION FEW FECES NO FECES 519 518-519 EYES RED DISCHARGE EYE-LEFT EYES 470, 477 518-519 SKIN & PELAGE YELLOW HAIR COAT 518-519 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 491 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 484 C91209 D 75 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519-520 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 991 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 282 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91210 M 75 BEHAVIOR HYPOACTIVE 523 EYES RED DISCHARGE EYES 523 SKIN & PELAGE COLD TO TOUCH ENTIRE BODY RED HAIR COAT HEAD-CRANIAL 523 512, 519, 523 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 407, 414, 498, 505 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 421, 428, 435, 442, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 372, 379, 449, 456, 463, 470, 92, 99, 106 183, 190, 197 274, 281, 288 386, 393, 400 477, 484, 491 MASS(ES) VENTRAL-ABDOMINAL WEEK 51 351C, 358C, 365C 992 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 283 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91211 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) INGUINAL REGION-LEFT WEEK 86 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 731C C91212 D 67 APPEARANCE HEAD TILT RIGHT THIN 456, 463, 467 449, 456, 463, 467 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 VENTRAL-ABDOMINAL-RIGHT WEEK 49 337, 344, 351C, 358C, 365C, 372C, 379C, 386C, 393C, 407C, 414C 421C, 428C, 435C, 442C, 449C, 456C, 463C, 467C 400C 993 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 284 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91213 M 77 APPEARANCE HEAD TILT RIGHT 535 THIN 535 EXCRETION FEW FECES 519, 526, 529, 533, 535 EYES RED DISCHARGE EYES 529, 533, 535 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT 519, 526, 529, 533, 535 529, 533, 535 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 994 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 285 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91214 M 99 APPEARANCE MALOCCLUSION THIN 589, 596, 603, 610 659, 666, 673, 680, 683, 687 EYES RED DISCHARGE EYE-LEFT 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603 638, 642, 645, 652, 659, 666, 673, 680, 683, 687 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330 MASS(ES) INGUINAL REGION-LEFT WEEK 75 WEEK 84 INGUINAL REGION-RIGHT WEEK 84 VENTRAL-ABDOMINAL-LEFT WEEK 64 WEEK 75 VENTRAL-CERVICAL WEEK 64 519C, 526C, 533C, 540C, 547C, 554C, 561C 582C, 589C, 596C, 603C, 610C 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 642C, 645C 652C, 659C, 666C, 673C, 680C, 683C, 687C 442C, 519C, 596C, 666C, 526C 449C, 526C, 603C, 673C, 456C, 533C, 610C, 680C, 463C, 540C, 617C, 683C, 470C, 547C, 624C, 687C 477C, 554C, 631C, 484C, 561C, 638C, 491C, 568C, 642C, 498C, 575C, 645C, 505C, 582C, 652C, 512C 589C 659C 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C 995 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 286 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91214 M 99 MASS(ES) VENTRAL-CERVICAL WEEK 64 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 638C, 642C, 645C, 652C WEEK 95 659C, 666C, 673C, 680C, 683C, 687C C91215 D 54 APPEARANCE MALOCCLUSION 372, 378 SKIN & PELAGE COLD TO TOUCH LIMBS-HIND 372, 378 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 C91216 T 105 APPEARANCE THIN 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 996 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 287 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91216 T 105 MASS(ES) INGUINAL REGION-RIGHT WEEK 98 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729-730C C91217 M 68 APPEARANCE THIN 470, 472 EXCRETION FEW FECES NO FECES 463 470, 472 EYES RED DISCHARGE EYES 470, 472 RESPIRATION IRREGULAR 472 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL 463, 470, 472 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456 997 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 288 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91218 M 97 APPEARANCE HEAD TILT LEFT HUNCHED MALOCCLUSION THIN 638, 645, 652, 659, 666, 673 617, 624, 631, 638, 645, 652, 659, 666, 673 274 659, 666, 673 BEHAVIOR ATAXIC HYPOACTIVE 673 617, 624 DISCHARGE NASAL RED IN COLOR 673 EXCRETION FEW FECES NO FECES 589, 617, 673 666 SKIN & PELAGE SORE/SCAB MOUTH 29 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 120, 127, 211, 218, 309, 316, 400, 407, 491, 498, 582, 603, 22, 36, 43, 50, 57, 134, 141, 148, 155, 225, 232, 239, 246, 323, 330, 337, 344, 414, 421, 428, 435, 505, 512, 519, 526, 610 64, 71, 78, 85, 92, 162, 169, 176, 183, 253, 260, 267, 281, 351, 358, 365, 372, 442, 449, 456, 463, 533, 540, 547, 554, 99, 106, 113 190, 197, 204 288, 295, 302 379, 386, 393 470, 477, 484 561, 568, 575 998 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 289 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91219 M 88 APPEARANCE HUNCHED MALOCCLUSION THIN 610 197, 204, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 575, 582, 589, 596, 600, 603, 609-610 610 BEHAVIOR HYPOACTIVE 610 EXCRETION FEW FECES 600, 603, 609-610 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 582, 589, 596, 600, 603, 609-610 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 218, 225, 309, 316, 400 22, 29, 36, 43, 50, 127, 134, 141, 148, 232, 239, 246, 253, 323, 330, 337, 344, 57, 64, 71, 78, 85, 155, 162, 169, 176, 260, 267, 274, 281, 351, 358, 365, 372, 92, 99, 106 183, 190, 211 288, 295, 302 379, 386, 393 MASS(ES) VENTRAL-ABDOMINAL WEEK 88 610C 999 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 290 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91220 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 VENTRAL-ABDOMINAL WEEK 82 VENTRAL-ABDOMINAL-LEFT WEEK 82 VENTRAL-ABDOMINAL-RIGHT WEEK 97 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C 732C 568, 575, 582, 589, 596C, 603C, 610C, 617C, 624C, 631C, 638C 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 722C, 729C, 732C 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 722C, 729C, 732C 673, 680, 687, 694C, 701C, 708C, 715C, 722C, 729C, 732C C91221 T 105 APPEARANCE MALOCCLUSION 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323 330, 337, 344, 351, 358, 365, 372, 400, 407, 414, 421, 428, 435 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708 715, 722, 729-730 1000 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 291 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91221 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 379, 386, 393 C91222 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91223 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C91224 M 97 APPEARANCE MALOCCLUSION THIN 589, 596, 603, 610 659, 666, 673, 676 DISCHARGE GENITAL RED IN COLOR 676 NASAL RED IN COLOR 676 EXCRETION FEW FECES 656, 659, 666, 673, 676 EYES RED DISCHARGE EYES 656, 676 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL 676 1001 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 292 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91224 M 97 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 568, 575, 582, 652 MASS(ES) INGUINAL REGION-LEFT WEEK 87 INGUINAL REGION-RIGHT WEEK 72 WEEK 87 603, 610, 617, 624, 631, 638, 645 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C 603, 610, 617, 624, 631, 638, 645 C91225 M 84 APPEARANCE MALOCCLUSION MISSING TEETH SWOLLEN VENTRAL-ABDOMINAL THIN 15, 22, 29, 36, 43, 120, 127, 134, 141, 211, 218, 225, 232, 302, 309, 316, 323, 393, 400, 407, 414, 484, 491, 498, 505, 575, 582, 585 50, 57, 64, 71, 78, 148, 155, 162, 169, 239, 246, 253, 260, 330, 337, 344, 351, 421, 428, 435, 442, 512, 519, 526, 533, 85, 92, 99, 106, 113 176, 183, 190, 197, 204 267, 274, 281, 288, 295 358, 365, 372, 379, 386 449, 456, 463, 470, 477 540, 547, 554, 561, 568 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 554, 561, 568, 575, 582, 585 582, 585 568, 575, 582, 585 EXCRETION FEW FECES 568, 575, 582, 585 1002 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 293 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91225 M 84 EYES PALE EYES 585 SKIN & PELAGE COLD TO TOUCH ENTIRE BODY PALE ENTIRE BODY 582, 585 582, 585 NORMAL NO REMARKABLE OBSERVATIONS 1, 8 C91226 M 86 APPEARANCE HEAD TILT LEFT RIGHT THIN 596 582, 589 582, 589, 596 BEHAVIOR HYPOACTIVE 596 EYES RED DISCHARGE EYES 568, 575, 582 RESPIRATION AUDIBLE 596 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 1003 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 294 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91226 M 86 NORMAL NO REMARKABLE OBSERVATIONS 204, 211, 218, 225, 232 MASS(ES) AXILLARY REGION-LEFT WEEK 59 AXILLARY REGION-RIGHT WEEK 38 INGUINAL REGION-LEFT WEEK 64 INGUINAL REGION-RIGHT WEEK 74 VENTRAL-ABDOMINAL WEEK 35 VENTRAL-CERVICAL-RIGHT WEEK 78 407, 414, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C 561C, 568C, 575C, 582C, 589C, 596C 260, 267, 274C, 281C, 288C, 295C, 302C, 309C, 316C, 323C, 330C 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C 596C 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C 589C, 596C 239C, 246C, 253C, 260C, 267C, 274C, 281C, 288C, 295C, 302C, 309C 316C, 323C 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C C91227 T 105 APPEARANCE MALOCCLUSION 589, 596, 603 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 1004 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 295 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91227 T 105 NORMAL NO REMARKABLE OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575 MASS(ES) INGUINAL REGION-LEFT WEEK 86 INGUINAL REGION-RIGHT WEEK 84 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729-730C 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 729-730C C91228 M 94 EYES RED DISCHARGE EYES 638, 645, 652 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL RED HAIR COAT HEAD-ENTIRE 638, 645, 652 624, 631, 638, 645, 652 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 421, 428, 512, 519, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 435, 442, 449, 456, 526, 533, 540, 547, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 372, 379, 386, 393, 463, 470, 477, 484, 554, 561 92, 99, 106 183, 190, 197 274, 281, 288 400, 407, 414 491, 498, 505 1005 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 296 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91228 M 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 82 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C 645C, 652C VENTRAL-ABDOMINAL WEEK 49 337C, 344C, 351C, 358C, 365C C91229 M 94 APPEARANCE THIN 645, 652 RESPIRATION IRREGULAR 652 SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL-LEFT 596, 603, 610, 617, 624, 631, 638, 645, 652 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 505, 512, 519, 526, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 533, 540, 547, 554, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 561 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 WEEK 82 491, 498 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C 645C, 652C 1006 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 297 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91230 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91231 M 92 BEHAVIOR HYPOACTIVE 638-639 DISCHARGE NASAL RED IN COLOR 639 EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL 638-639 638-639 RESPIRATION LABORED 639 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 631, 638-639 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 512, 519, 603, 610, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 526, 533, 540, 547, 617, 624 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 554, 561, 568, 575, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 498, 505 582, 589, 596 1007 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 298 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91231 M 92 WART LIKE LESION(S) HEAD-MAXILLARY-RIGHT WEEK 67 463, 470, 477, 484, 491 C91232 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 731 C91233 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C91234 D 66 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 459 C91235 T 105 EYES RED DISCHARGE EYE-LEFT 568 SKIN & PELAGE SORE/SCAB HEAD-MAXILLARY-LEFT 568 MOUTH 582 1008 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 299 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91235 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 575, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 C91236 T 105 APPEARANCE MALOCCLUSION 589, 596, 603, 610 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 400, 407, 491, 498, 582, 617, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 330, 337, 344, 414, 421, 428, 435, 505, 512, 519, 526, 624, 631 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 351, 358, 365, 372, 442, 449, 456, 463, 533, 540, 547, 554, 92, 99, 106 183, 190, 197 274, 281, 288 379, 386, 393 470, 477, 484 561, 568, 575 MASS(ES) AXILLARY REGION-RIGHT WEEK 93 INGUINAL REGION-LEFT WEEK 94 VENTRAL-ABDOMINAL WEEK 92 VENTRAL-ABDOMINAL-LEFT WEEK 46 645, 652, 659, 666, 673, 680, 687, 694C, 701C, 708C, 715C, 722C 729-730C 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 638C 316, 323 1009 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 300 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91237 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91238 M 68 APPEARANCE THIN 456, 463, 470 EXCRETION FEW FECES 463, 470 EYES RED DISCHARGE EYES 456, 463, 470 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 C91239 M 58 APPEARANCE MALOCCLUSION 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 EYES RED DISCHARGE EYE-RIGHT 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218 SKIN & PELAGE SORE/SCAB AXILLARY REGION-RIGHT 323, 330, 337, 344, 351, 358, 365 1010 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 301 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91239 M 58 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127 MASS(ES) AXILLARY REGION-LEFT WEEK 47 AXILLARY REGION-RIGHT WEEK 35 323, 330C, 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C 400C 239C, 246C, 253C, 260C, 267C, 274C, 281C, 288C, 295C, 302C, 309C 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C 393C, 400C C91240 M 79 APPEARANCE MALOCCLUSION 85, 92, 99, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533 540, 547, 550 RESPIRATION LABORED 550 SKIN & PELAGE YELLOW HAIR COAT 435 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78 1011 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 302 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91240 M 79 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 512, 519, 526, 533, 540, 547C, 550C C91241 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C91242 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729-730 C91243 T 105 SKIN & PELAGE SORE/SCAB DORSAL 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 414, 421, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 428, 435, 442, 449, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 456, 463 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 MASS(ES) AXILLARY REGION-LEFT WEEK 68 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C 1012 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 303 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91243 T 105 MASS(ES) AXILLARY REGION-LEFT WEEK 68 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C 701C, 708C, 715C, 722C, 729C, 731C INGUINAL REGION-LEFT WEEK 89 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C 694C, 701C, 708C, 715C, 722C, 729C, 731C INGUINAL REGION-RIGHT WEEK 93 645, 652, 659, 666, 673C, 680C, 687C, 694C, 701C, 708C, 715C 722C, 729C, 731C VENTRAL-ABDOMINAL-RIGHT WEEK 56 386, 393, 400, 407 C91244 M 67 APPEARANCE MALOCCLUSION 241, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414 421, 428, 435, 442, 449, 456, 463, 467 BEHAVIOR HYPOACTIVE 467 VOCALIZATION 467 DISCHARGE NASAL RED IN COLOR 241 EYES RED DISCHARGE EYES 241, 246 1013 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 304 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91244 M 67 RESPIRATION AUDIBLE 467 SKIN & PELAGE PALE EARS 241, 246 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239 C91245 M 96 APPEARANCE MALOCCLUSION 589, 596, 603 BEHAVIOR HYPOACTIVE 668 EXCRETION FEW FECES 659, 666, 668 EYES RED DISCHARGE EYES 668 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT 668 659, 666, 668 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 1014 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 305 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91245 M 96 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 610, 617, 624, 631, 638, 645, 652 C91246 M 87 APPEARANCE HUNCHED THIN 582, 589, 596, 600, 603-604 603-604 EXCRETION NO FECES 600, 603-604 EYES RED DISCHARGE EYES 603-604 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463 MASS(ES) AXILLARY REGION-LEFT WEEK 68 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 600C, 603-604C 1015 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 306 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91247 M 73 APPEARANCE THIN 470, 477, 484, 491, 498, 505, 508 BEHAVIOR RECUMBENT STERNAL 508 DISCHARGE ORAL RED IN COLOR 508 EXCRETION NO FECES 470, 508 EYES RED DISCHARGE EYES 470, 477, 484, 491, 498, 505, 508 RESPIRATION AUDIBLE 508 LABORED 508 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL 456, 463, 470, 477, 484, 491 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 316, 323, 407, 414, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 330, 337, 344, 351, 421, 428, 435 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 358, 365, 372, 379, 92, 99, 106 183, 190, 197 295, 302, 309 386, 393, 400 1016 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 307 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91247 M 73 MASS(ES) INGUINAL REGION-LEFT WEEK 64 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 508C VENTRAL-ABDOMINAL WEEK 40 274C, 281C, 288C VENTRAL-ABDOMINAL-RIGHT WEEK 70 484C, 491C, 498C, 505C, 508C C91248 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C91249 M 97 APPEARANCE MALOCCLUSION THIN 484, 491, 498, 505 657, 659, 666, 673 BEHAVIOR HYPOACTIVE 673 DISCHARGE NASAL RED IN COLOR 673 EXCRETION FEW FECES 652, 657, 659, 666, 673 EYES PALE EYES 638, 645, 652, 657, 659, 666, 673 RESPIRATION LABORED 673 1017 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 308 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91249 M 97 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 638, 645, 652, 657 PERINEAL AREA 645, 652, 657, 659, 666 VENTRAL-ABDOMINAL 673 YELLOW HAIR COAT 673 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 512, 596, 603, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 519, 526, 533, 540, 610, 617, 624, 631 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 547, 554, 561, 568, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 575, 582, 589 C91250 M 73 APPEARANCE MALOCCLUSION 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 449, 456, 463, 470, 477, 484, 491, 498, 505 SKIN & PELAGE SORE/SCAB AXILLARY REGION-RIGHT 498, 505 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169 1018 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 309 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 3 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91250 M 73 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 260C, 267C, 274C, 281C, 288C, 295C, 302C, 309C, 316C, 323C, 330C 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C 491C, 498C, 505C 1019 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 310 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91191 F2 638 92 MASS(ES) AXILLARY REGION-LEFT WEEK 92 15 X 15MM 645 93 TO 680 98 MASS(ES) AXILLARY REGION-LEFT WEEK 92 20 X 20MM 526 76 TO 533 77 MASS(ES) INGUINAL REGION-RIGHT WEEK 76 15 X 15MM 575 83 TO 582 84 MASS(ES) INGUINAL REGION-RIGHT WEEK 83 30 X 20MM 589 85 TO 596 86 MASS(ES) INGUINAL REGION-RIGHT WEEK 83 35 X 25MM 603 87 TO 652 94 MASS(ES) INGUINAL REGION-RIGHT WEEK 83 50 X 40MM 659 95 TO 666 96 MASS(ES) INGUINAL REGION-RIGHT WEEK 83 55 X 70MM 673 97 TO 680 98 MASS(ES) INGUINAL REGION-RIGHT WEEK 83 70 X 70MM 1020 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 311 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91193 F2 596 86 TO 603 87 MASS(ES) AXILLARY REGION-LEFT WEEK 85 10 X 10MM 610 88 TO 631 91 MASS(ES) AXILLARY REGION-LEFT WEEK 85 25 X 20MM C91194 F2 491 71 TO 519 75 MASS(ES) AXILLARY REGION-RIGHT WEEK 71 20 X 20MM 526 76 TO 561 81 MASS(ES) AXILLARY REGION-RIGHT WEEK 71 30 X 35MM 568 82 MASS(ES) AXILLARY REGION-RIGHT WEEK 71 60 X 40MM 575 83 TO 596 86 MASS(ES) AXILLARY REGION-RIGHT WEEK 71 60 X 60MM 603 87 TO 652 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 71 70 X 70MM 659 95 TO 728 104 MASS(ES) AXILLARY REGION-RIGHT WEEK 71 65 X 60MM 1021 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 312 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91195 F2 603 87 TO 617 89 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 10 X 20MM 624 90 TO 638 92 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 20 X 20MM 645 93 TO 694 100 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 45 X 35MM C91196 F2 554 80 TO 561 81 MASS(ES) INGUINAL REGION-RIGHT WEEK 80 10 X 10MM 565 81 MASS(ES) INGUINAL REGION-RIGHT WEEK 80 25 X 20MM 568 82 TO 575 83 MASS(ES) INGUINAL REGION-RIGHT WEEK 80 30 X 30MM 579 83 MASS(ES) INGUINAL REGION-RIGHT WEEK 80 50 X 30MM 579 83 MASS(ES) INGUINAL REGION-RIGHT WEEK 80 50 X 30MM 1022 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 313 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91196 F2 579 83 MASS(ES) INGUINAL REGION-RIGHT WEEK 80 50 X 30MM C91200 F2 575 83 TO 582 84 MASS(ES) HEAD WEEK 82 10 X 20MM 589 85 MASS(ES) HEAD WEEK 82 10 X 25MM 596 86 MASS(ES) HEAD WEEK 82 10 X 30MM 603 87 TO 607 87 MASS(ES) HEAD WEEK 82 30 X 35MM C91201 F2 582 84 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 10 X 20MM 589 85 TO 617 89 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 15 X 25MM 624 90 TO 631 91 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 20 X 20MM 1023 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 314 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91201 F2 638 92 TO 645 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 30 X 25MM 652 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 35 X 30MM 659 95 TO 701 101 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 40 X 30MM 708 102 TO 722 104 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 60 X 50MM 729 105 TO 730 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 60 X 45MM 673 97 TO 701 101 MASS(ES) INGUINAL REGION-RIGHT WEEK 97 10 X 10MM 708 102 TO 730 105 MASS(ES) INGUINAL REGION-RIGHT WEEK 97 50 X 30MM 337 49 TO 351 51 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 49 25 X 20MM 1024 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 315 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91201 F2 729 105 TO 730 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 105 20 X 20MM C91202 F2 526 76 TO 561 81 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 30 X 20MM 568 82 TO 590 85 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 40 X40MM 582 84 TO 590 85 MASS(ES) INGUINAL REGION-LEFT WEEK 84 20 X 10MM 582 84 TO 590 85 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 84 10 X 15MM C91203 F2 449 65 TO 456 66 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 10 X 10MM 470 68 TO 512 74 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 30 X 30MM 519 75 TO 532 76 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 40 X 40MM 1025 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 316 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91204 F2 239 35 TO 302 44 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 30 X 25MM 309 45 TO 323 47 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 50 X 40MM 330 48 TO 365 53 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 45 X 30MM 372 54 TO 426 61 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 45 X 35MM C91210 F2 351 51 TO 365 53 MASS(ES) VENTRAL-ABDOMINAL WEEK 51 15 X 15MM C91211 F2 596 86 TO 645 93 MASS(ES) INGUINAL REGION-LEFT WEEK 86 30 X 30MM 652 94 TO 722 104 MASS(ES) INGUINAL REGION-LEFT WEEK 86 20 X 20MM 729 105 TO 731 105 MASS(ES) INGUINAL REGION-LEFT WEEK 86 20 X 40MM 1026 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 317 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91212 F2 351 51 TO 365 53 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 10 X 10MM 372 54 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 15 X 15MM 379 55 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 20 X 15MM 386 56 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 20 X 15MM 393 57 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 20 X 15MM 407 59 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 20 X 15MM 414 60 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 10 X 10MM 421 61 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 10 X 10MM 1027 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 318 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91212 F2 428 62 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 10 X 10M 435 63 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 10 X 10MM 442 64 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 10 X 10MM 449 65 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 10 X 10MM 456 66 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 10 X 10MM 463 67 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 10 X 10MM 467 67 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 10 X 10MM 400 58 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 49 20 X 15MM 1028 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 319 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91214 F2 519 75 TO 561 81 MASS(ES) INGUINAL REGION-LEFT WEEK 75 20 X 20MM 582 84 TO 610 88 MASS(ES) INGUINAL REGION-LEFT WEEK 84 10 X 10MM 582 84 TO 687 99 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 10 X 10MM 442 64 TO 463 67 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 64 15 X 15MM 470 68 TO 484 70 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 64 20 X 25MM 491 71 TO 512 74 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 64 30 X 30MM 519 75 TO 547 79 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 64 35 X 40MM 554 80 TO 561 81 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 64 35 X 55MM 1029 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 320 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91214 F2 568 82 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 64 40 X 70MM 589 85 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 64 60 X 80MM 603 87 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 64 60 X 90MM 638 92 TO 652 94 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 64 70 X 100MM 659 95 TO 666 96 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 64 90 X 50MM 673 97 TO 687 99 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 64 50 X 90MM 526 76 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 75 35 X 40MM 442 64 TO 463 67 MASS(ES) VENTRAL-CERVICAL WEEK 64 10 X 10MM 1030 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 321 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91214 F2 470 68 TO 512 74 MASS(ES) VENTRAL-CERVICAL WEEK 64 25 X 25MM 519 75 MASS(ES) VENTRAL-CERVICAL WEEK 64 35 X 35MM 526 76 TO 540 78 MASS(ES) VENTRAL-CERVICAL WEEK 64 40 X 40MM 547 79 TO 561 81 MASS(ES) VENTRAL-CERVICAL WEEK 64 50 X 40MM 568 82 TO 582 84 MASS(ES) VENTRAL-CERVICAL WEEK 64 60 X 50MM 589 85 MASS(ES) VENTRAL-CERVICAL WEEK 64 70 X 60MM 596 86 MASS(ES) VENTRAL-CERVICAL WEEK 64 80 X 70MM 603 87 MASS(ES) VENTRAL-CERVICAL WEEK 64 85 X 75MM 1031 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 322 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91214 F2 610 88 TO 631 91 MASS(ES) VENTRAL-CERVICAL WEEK 64 90 X 80MM 638 92 TO 652 94 MASS(ES) VENTRAL-CERVICAL WEEK 64 100 X 85MM 659 95 TO 683 98 MASS(ES) VENTRAL-CERVICAL WEEK 95 75 X 90MM 687 99 TO 687 99 MASS(ES) VENTRAL-CERVICAL WEEK 95 85 X 90MM 687 99 MASS(ES) VENTRAL-CERVICAL WEEK 95 85 X 90MM C91216 F2 680 98 TO 701 101 MASS(ES) INGUINAL REGION-RIGHT WEEK 98 10 X 10MM 708 102 TO 722 104 MASS(ES) INGUINAL REGION-RIGHT WEEK 98 50 X 40MM 729 105 TO 730 105 MASS(ES) INGUINAL REGION-RIGHT WEEK 98 50 X 30MM 1032 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 323 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91219 F2 610 88 TO 610 88 MASS(ES) VENTRAL-ABDOMINAL WEEK 88 10 X 10MM C91220 F2 582 84 TO 596 86 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 10 X 15MM 603 87 TO 631 91 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 15 X 15MM 638 92 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 25 X 25MM 645 93 TO 659 95 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 30 X 25MM 666 96 TO 687 99 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 30 X 30MM 694 100 TO 732 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 40 X 35MM 596 86 MASS(ES) VENTRAL-ABDOMINAL WEEK 82 10 X 10MM 1033 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 324 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91220 F2 603 87 TO 610 88 MASS(ES) VENTRAL-ABDOMINAL WEEK 82 15 X 15MM 617 89 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL WEEK 82 20 X 20MM 638 92 TO 645 93 MASS(ES) VENTRAL-ABDOMINAL WEEK 82 25 X 25MM 652 94 MASS(ES) VENTRAL-ABDOMINAL WEEK 82 10 X 10MM 659 95 MASS(ES) VENTRAL-ABDOMINAL WEEK 82 20 X 25MM 666 96 TO 687 99 MASS(ES) VENTRAL-ABDOMINAL WEEK 82 30 X 30MM 694 100 TO 732 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 82 20 X 20MM 568 82 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 20 X 20MM 1034 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 325 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91220 F2 603 87 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 25 X 25MM 638 92 TO 645 93 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 30 X 40MM 652 94 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 30 X 30MM 666 96 TO 687 99 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 55 X 50MM 694 100 TO 701 101 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 65 X 55MM 708 102 TO 732 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 70 X 70MM 694 100 TO 701 101 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 97 25 X 25MM 708 102 TO 732 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 97 40 X 30MM 1035 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 326 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91224 F2 498 72 TO 561 81 MASS(ES) INGUINAL REGION-RIGHT WEEK 72 10 X 15MM C91226 F2 421 61 TO 435 63 MASS(ES) AXILLARY REGION-LEFT WEEK 59 15 X15MM 442 64 TO 561 81 MASS(ES) AXILLARY REGION-LEFT WEEK 59 35 X 30MM 568 82 TO 596 86 MASS(ES) AXILLARY REGION-LEFT WEEK 59 40 X 45MM 274 40 TO 400 58 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 10 X 10MM 442 64 TO 498 72 MASS(ES) INGUINAL REGION-LEFT WEEK 64 15 X 15MM 505 73 TO 512 74 MASS(ES) INGUINAL REGION-LEFT WEEK 64 20 X 20MM 519 75 TO 561 81 MASS(ES) INGUINAL REGION-LEFT WEEK 64 30 X 50MM 1036 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 327 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91226 F2 568 82 TO 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 64 40 X 60MM 512 74 MASS(ES) INGUINAL REGION-RIGHT WEEK 74 15 X 15MM 519 75 TO 568 82 MASS(ES) INGUINAL REGION-RIGHT WEEK 74 20 X 20MM 575 83 TO 589 85 MASS(ES) INGUINAL REGION-RIGHT WEEK 74 30 X 30MM 596 86 TO 596 86 MASS(ES) INGUINAL REGION-RIGHT WEEK 74 40 X 40MM 596 86 MASS(ES) INGUINAL REGION-RIGHT WEEK 74 40 X 40MM 239 35 TO 323 47 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 20 X 20MM 540 78 TO 561 81 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 78 20 X 20MM 1037 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 328 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91226 F2 568 82 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 78 20 X 30MM 575 83 TO 596 86 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 78 30 X 30MM C91227 F2 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 86 25 X 25MM 603 87 TO 631 91 MASS(ES) INGUINAL REGION-LEFT WEEK 86 25 X 35MM 638 92 TO 645 93 MASS(ES) INGUINAL REGION-LEFT WEEK 86 50 X 40MM 652 94 TO 680 98 MASS(ES) INGUINAL REGION-LEFT WEEK 86 60 X 50MM 687 99 MASS(ES) INGUINAL REGION-LEFT WEEK 86 60 X 60MM 694 100 TO 701 101 MASS(ES) INGUINAL REGION-LEFT WEEK 86 70 X 65MM 1038 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 329 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91227 F2 708 102 TO 730 105 MASS(ES) INGUINAL REGION-LEFT WEEK 86 85 X 65MM 582 84 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 15 X 10 MM 589 85 TO 596 86 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 25 X 25MM 603 87 TO 617 89 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 35 X 35MM 624 90 TO 631 91 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 40 X 40MM 638 92 TO 680 98 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 50 X 45MM 687 99 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 70 X 60MM 694 100 TO 701 101 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 85 X 60 1039 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 330 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91227 F2 708 102 TO 730 105 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 100 X 70MM C91228 F2 568 82 TO 589 85 MASS(ES) AXILLARY REGION-RIGHT WEEK 82 20 X 20MM 596 86 TO 617 89 MASS(ES) AXILLARY REGION-RIGHT WEEK 82 30 X 25MM 624 90 TO 652 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 82 25 X 20MM 337 49 TO 365 53 MASS(ES) VENTRAL-ABDOMINAL WEEK 49 10 X 10MM C91229 F2 568 82 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 10 X10MM 575 83 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 20 X 20MM 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 25 X 30MM 1040 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 331 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91229 F2 589 85 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 30 X 35MM 603 87 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 35 X 35MM 638 92 TO 652 94 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 50 X 45MM C91236 F2 694 100 TO 730 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 93 15 X 15MM 652 94 MASS(ES) INGUINAL REGION-LEFT WEEK 94 10 X 10MM 659 95 TO 687 99 MASS(ES) INGUINAL REGION-LEFT WEEK 94 20 X 20MM 694 100 TO 722 104 MASS(ES) INGUINAL REGION-LEFT WEEK 94 25 X 25MM 638 92 MASS(ES) VENTRAL-ABDOMINAL WEEK 92 15 X 15MM 1041 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 332 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91239 F2 330 48 TO 400 58 MASS(ES) AXILLARY REGION-LEFT WEEK 47 10 X 10MM 239 35 TO 260 38 MASS(ES) AXILLARY REGION-RIGHT WEEK 35 10 X 10MM 267 39 TO 316 46 MASS(ES) AXILLARY REGION-RIGHT WEEK 35 15 X 20MM 323 47 TO 337 49 MASS(ES) AXILLARY REGION-RIGHT WEEK 35 20 X 20MM 344 50 TO 351 51 MASS(ES) AXILLARY REGION-RIGHT WEEK 35 20 X 25MM 358 52 TO 365 53 MASS(ES) AXILLARY REGION-RIGHT WEEK 35 30 X 30MM 372 54 TO 400 58 MASS(ES) AXILLARY REGION-RIGHT WEEK 35 45 X 45MM C91240 F2 547 79 TO 550 79 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 10 X 10MM 1042 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 333 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91243 F2 470 68 TO 484 70 MASS(ES) AXILLARY REGION-LEFT WEEK 68 10 X 10MM 491 71 TO 561 81 MASS(ES) AXILLARY REGION-LEFT WEEK 68 25 X 25MM 568 82 TO 582 84 MASS(ES) AXILLARY REGION-LEFT WEEK 68 40 X 40MM 589 85 TO 596 86 MASS(ES) AXILLARY REGION-LEFT WEEK 68 45 X 45MM 603 87 MASS(ES) AXILLARY REGION-LEFT WEEK 68 60 X 45MM 610 88 TO 638 92 MASS(ES) AXILLARY REGION-LEFT WEEK 68 65 X 55MM 645 93 MASS(ES) AXILLARY REGION-LEFT WEEK 68 70 X 60MM 652 94 MASS(ES) AXILLARY REGION-LEFT WEEK 68 85 X 60MM 1043 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 334 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91243 F2 659 95 MASS(ES) AXILLARY REGION-LEFT WEEK 68 75 X 50MM 666 96 TO 731 105 MASS(ES) AXILLARY REGION-LEFT WEEK 68 75 X 60MM 617 89 TO 652 94 MASS(ES) INGUINAL REGION-LEFT WEEK 89 20 X 15MM 659 95 TO 731 105 MASS(ES) INGUINAL REGION-LEFT WEEK 89 30 X 40MM 673 97 TO 680 98 MASS(ES) INGUINAL REGION-RIGHT WEEK 93 15 X 15MM 687 99 TO 731 105 MASS(ES) INGUINAL REGION-RIGHT WEEK 93 25 X 25MM C91246 F2 470 68 TO 477 69 MASS(ES) AXILLARY REGION-LEFT WEEK 68 15 X 15MM 484 70 TO 491 71 MASS(ES) AXILLARY REGION-LEFT WEEK 68 20 X 20MM 1044 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 335 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91246 F2 498 72 TO 512 74 MASS(ES) AXILLARY REGION-LEFT WEEK 68 30 X 20MM 519 75 TO 540 78 MASS(ES) AXILLARY REGION-LEFT WEEK 68 40 X 30MM 547 79 TO 561 81 MASS(ES) AXILLARY REGION-LEFT WEEK 68 50 X 30MM 568 82 MASS(ES) AXILLARY REGION-LEFT WEEK 68 60 X 45MM 575 83 TO 582 84 MASS(ES) AXILLARY REGION-LEFT WEEK 68 65 X 60MM 589 85 MASS(ES) AXILLARY REGION-LEFT WEEK 68 75 X 70MM 596 86 TO 600 86 MASS(ES) AXILLARY REGION-LEFT WEEK 68 100 X 70MM 603 87 TO 604 87 MASS(ES) AXILLARY REGION-LEFT WEEK 68 105 X 75MM 1045 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 336 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91247 F2 442 64 TO 477 69 MASS(ES) INGUINAL REGION-LEFT WEEK 64 15 X 15MM 484 70 TO 505 73 MASS(ES) INGUINAL REGION-LEFT WEEK 64 25 X 25MM 508 73 TO 508 73 MASS(ES) INGUINAL REGION-LEFT WEEK 64 30 X 30MM 274 40 TO 288 42 MASS(ES) VENTRAL-ABDOMINAL WEEK 40 10 X 20MM 484 70 TO 508 73 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 20 X 20MM C91250 F2 260 38 TO 267 39 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 10 X 10MM 274 40 TO 309 45 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 20 X 20MM 316 46 TO 323 47 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 35 X 35MM 1046 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 337 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91250 F2 330 48 TO 344 50 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 40 X 35MM 351 51 TO 372 54 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 50 X 45MM 379 55 TO 400 58 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 60 X 55MM 407 59 TO 414 60 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 70 X 60MM 421 61 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 80 X 65MM 428 62 TO 435 63 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 80 X 80MM 442 64 TO 498 72 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 90 X 85MM 505 73 TO 505 73 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 100 X 100MM 1047 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 338 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91250 F2 505 73 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 90 X 85MM 1048 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 339 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91251 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91252 T 105 APPEARANCE MALOCCLUSION 589, 596, 603, 610, 617, 624, 631, 638, 645 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 113, 120, 127, 134, 141, 148, 204, 211, 218, 225, 232, 239, 295, 302, 309, 316, 323, 330, 386, 393, 400, 407, 414, 421, 477, 484, 491, 498, 505, 512, 568, 575, 582, 652, 659, 666, 722, 729-730 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 673, 680, 687, 694, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 701, 708, 715 C91253 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 105 729-730C 1049 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 340 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91254 T 105 APPEARANCE MALOCCLUSION 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687 694, 701, 708, 715, 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C91255 M 74 APPEARANCE HUNCHED 512-513 BEHAVIOR HYPOACTIVE RECUMBENT STERNAL 512-513 513 EYES SQUINTED PERI-ORBITAL 512-513 RESPIRATION LABORED 513 SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT 498, 505, 512-513 1050 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 341 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91255 M 74 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421 MASS(ES) AXILLARY REGION-LEFT WEEK 64 VENTRAL-ABDOMINAL-RIGHT WEEK 62 442, 449, 456, 463, 470C, 477C, 484C, 491C, 498C, 505C, 512-513C 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C 505C, 512-513C C91256 T 105 APPEARANCE SWOLLEN PAW-HIND-LEFT 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) VENTRAL-ABDOMINAL WEEK 90 624, 631, 638, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C 701C, 708C, 715C, 722C, 729C, 731C 1051 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 342 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91257 M 83 APPEARANCE HUNCHED MALOCCLUSION THIN 579 554, 561, 568-570, 575, 579 540, 547, 554, 561, 568-570, 575, 579 BEHAVIOR HYPOACTIVE 568-570, 575, 579 EXCRETION FEW FECES 570, 575 EYES RED DISCHARGE EYE-LEFT 64, 71, 120, 127, 134, 162, 169, 176, 183, 190, 197, 204, 211 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568-570 575, 579 SKIN & PELAGE YELLOW HAIR COAT 579 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 78, 85, 92, 99, 106, 113, 141 148, 155 MASS(ES) VENTRAL-CERVICAL WEEK 68 470, 477 1052 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 343 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91258 M 86 APPEARANCE HUNCHED 600 THIN 600 EXCRETION FEW FECES 600 SKIN & PELAGE YELLOW HAIR COAT 600 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) AXILLARY REGION-LEFT WEEK 75 VENTRAL-ABDOMINAL-LEFT WEEK 71 VENTRAL-THORACIC-LEFT WEEK 79 519C, 526C, 533C 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C 568C, 575C, 582C, 589C, 596C, 600C 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 600C 1053 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 344 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91259 D 63 APPEARANCE HEAD TILT RIGHT THIN 435, 438 421, 428, 435, 438 BEHAVIOR ATAXIC HYPOACTIVE 435, 438 428, 435, 438 EYES RED DISCHARGE EYE-RIGHT EYES 435, 438 393, 400, 407, 414, 421, 428 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 C91260 M 73 APPEARANCE THIN 498, 505, 509 BEHAVIOR HYPOACTIVE 509 RECUMBENT LATERAL 509 EXCRETION FEW FECES 491, 498, 505, 509 1054 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 345 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91260 M 73 EYES RED DISCHARGE EYES 491, 498, 505, 509 RESPIRATION LABORED 509 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA RED HAIR COAT HEAD-ENTIRE 498, 505, 509 491, 498, 505, 509 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C91261 D 99 APPEARANCE MALOCCLUSION THIN 463, 470, 477, 484, 491 673, 680, 687, 689 SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 689 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 1055 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 346 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91261 D 99 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 498, 505 512, 519, 526, 533, 540, 547, 554, 561 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C, 689C C91262 M 89 APPEARANCE DEHYDRATED HUNCHED THIN 617-618 617-618 610, 617-618 EXCRETION NO FECES 617-618 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT 610, 617-618 617-618 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 463, 547, 554 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 470, 477, 484, 491, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 498, 505, 512, 519, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 526, 533, 540 1056 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 347 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91262 M 89 MASS(ES) VENTRAL-ABDOMINAL WEEK 57 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 VENTRAL-THORACIC WEEK 81 561, 568, 575, 582, 589, 596, 603, 610, 617-618 C91263 M 72 APPEARANCE THIN 484, 491, 498 BEHAVIOR ATAXIC 498 HYPOACTIVE 498 EXCRETION FEW FECES 484, 498 EYES RED DISCHARGE EYES 456, 463, 484, 498 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA VENTRAL-ABDOMINAL YELLOW HAIR COAT 498 463 456, 463 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141 1057 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 348 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91263 M 72 MASS(ES) AXILLARY REGION-LEFT WEEK 33 225C, 232C, 239C, 246C, 253C AXILLARY REGION-RIGHT WEEK 31 211, 218, 225 VENTRAL-ABDOMINAL-LEFT WEEK 22 148, 155, 162, 169, 176C, 183C, 190C, 197C, 204C, 211C, 218C 225C, 232C, 239C, 246C, 253C, 260C, 267C, 274C, 281C, 288C, 295C 302C, 309C, 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C, 372C 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C 456C, 463C, 470C, 477C, 484C, 491C, 498C C91264 T 105 SKIN & PELAGE ALOPECIA 568, 575, 617, 624, 631 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 582, 638 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) AXILLARY REGION-LEFT WEEK 97 AXILLARY REGION-RIGHT WEEK 85 WEEK 93 673, 680, 687C, 694C, 701C, 708C, 715C, 722C, 729-730C 589, 596, 603, 610 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 722C, 729-730C 1058 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 349 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91265 M 94 APPEARANCE HUNCHED MALOCCLUSION THIN 653 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652-653 653 BEHAVIOR ATAXIC 653 HYPOACTIVE 653 EXCRETION FEW FECES 645, 652-653 SKIN & PELAGE YELLOW HAIR COAT 653 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120 C91266 M 104 APPEARANCE HEAD TILT RIGHT THIN 723 713, 715, 722-723 EXCRETION FEW FECES NO FECES 713 715, 722-723 1059 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 350 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91266 M 104 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL 713, 715, 722-723 BLACK SKIN VENTRAL-ABDOMINAL-LEFT 713, 715, 722-723 SORE/SCAB PAW-HIND-LEFT 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694, 701, 708, 713, 715, 722-723 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 379, 386, 470, 477, 561, 568, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 393, 400, 407, 414, 484, 491, 498, 505, 575 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 421, 428, 435, 442, 512, 519, 526, 533, 92, 99, 106 183, 190, 197 358, 365, 372 449, 456, 463 540, 547, 554 MASS(ES) AXILLARY REGION-RIGHT WEEK 97 INGUINAL REGION-RIGHT WEEK 89 VENTRAL-ABDOMINAL-RIGHT WEEK 40 673, 680, 687, 694C, 701C, 708C, 713C, 715C, 722-723C 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C 694C, 701C, 708C, 713C, 715C, 722-723C 274C, 281C, 288C, 295C, 302C, 309C, 316C, 323C, 330C, 337, 344 351 1060 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 351 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91267 M 67 SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL-RIGHT SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 358, 365 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 463-464 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 38 VENTRAL-CERVICAL-LEFT WEEK 46 260C, 267C, 274C, 281C, 288C, 295C, 302C, 309C, 316C, 323C, 330C 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463-464C 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C 463-464C C91268 M 97 EYES DISCOLORED/DARK RED EYE-LEFT 645, 652, 659, 666, 673 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 673 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 1061 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 352 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91268 M 97 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 80 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 652C, 659C, 666C, 673C C91269 M 83 APPEARANCE DEHYDRATED HEAD TILT LEFT THIN 568-569 578 566, 568-569, 575, 578 BEHAVIOR HYPOACTIVE 566, 568-569, 575, 578 DISCHARGE NASAL RED IN COLOR 557, 566, 568-569, 575, 578 EXCRETION FEW FECES NO FECES 551, 554, 575 557, 566, 568-569, 578 EYES RED DISCHARGE EYE-LEFT 578 1062 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 353 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91269 M 83 EYES RED DISCHARGE EYES 566, 568-569, 575 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT 547, 551, 554, 557 551, 554, 557, 561, 566, 568-569, 575, 578 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C91270 M 59 APPEARANCE HEAD TILT LEFT THIN 407 386, 393, 400, 407 BEHAVIOR CIRCLING LEFT 407 EXCRETION NO FECES 386 EYES RED DISCHARGE EYES 386 1063 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 354 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91270 M 59 RESPIRATION LABORED 407 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL 386 386, 393, 400, 407 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 C91271 M 94 APPEARANCE HUNCHED THIN 645, 652-653 653 BEHAVIOR HYPOACTIVE 653 DISCHARGE GENITAL RED IN COLOR 653 EYES RED DISCHARGE EYE-LEFT 505, 645, 652-653 SKIN & PELAGE YELLOW HAIR COAT 653 1064 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 355 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91271 M 94 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 512 MASS(ES) AXILLARY REGION-LEFT WEEK 84 AXILLARY REGION-RIGHT WEEK 89 INGUINAL REGION-LEFT WEEK 75 WEEK 84 582, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652-653C 617, 624, 631, 638, 645, 652-653 519, 526, 533, 540, 547, 554C, 561C, 568C, 575C 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652-653C C91272 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91273 M 81 APPEARANCE HEAD TILT RIGHT 561 EYES RED DISCHARGE EYE-LEFT 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526 533, 540 1065 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 356 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91273 M 81 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435 MASS(ES) INGUINAL REGION-LEFT WEEK 76 526, 533, 540, 547, 554, 561 C91274 D 70 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 490 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C91275 M 69 APPEARANCE THIN 463, 470, 477, 480 BEHAVIOR HYPOACTIVE 442, 449 EXCRETION FEW FECES 470, 477, 480 EYES RED DISCHARGE EYE-LEFT 435, 442, 449, 456, 463, 470, 477, 480 1066 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 357 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91275 M 69 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 435, 442, 449, 456 ROUGH HAIR COAT 435, 442, 449, 456, 463, 470, 477, 480 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351 MASS(ES) AXILLARY REGION-LEFT WEEK 58 AXILLARY REGION-RIGHT WEEK 52 400, 407, 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C 477C, 480C 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C C91276 M 97 APPEARANCE MALOCCLUSION THIN 568, 575, 673 526 EYES PALE EYES RED DISCHARGE EYE-RIGHT 673 421, 428 SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL 673 1067 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 358 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91276 M 97 SKIN & PELAGE SORE/SCAB AXILLARY REGION-RIGHT 617, 624, 631, 638 LATERAL-ABDOMINAL-RIGHT 673 YELLOW HAIR COAT 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 638, 645, 652, 659, 666, 673 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 MASS(ES) AXILLARY REGION-LEFT WEEK 82 AXILLARY REGION-RIGHT WEEK 93 DORSAL-CERVICAL-LEFT WEEK 82 INGUINAL REGION-LEFT WEEK 88 VENTRAL-ABDOMINAL WEEK 72 VENTRAL-ABDOMINAL-RIGHT WEEK 62 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C 645C, 652C, 659C, 666C, 673C 645, 652, 659, 666, 673 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C 645C, 652C, 659C, 666C, 673C 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652C, 659C, 666C, 673C 428, 435, 442, 449, 456, 463, 470, 477, 484C, 491C, 498C, 505C 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C 1068 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 359 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91276 M 97 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 62 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C 666C, 673C C91277 M 72 APPEARANCE HUNCHED MALOCCLUSION THIN 498 141, 148, 155, 162, 169, 176, 183, 190 498 EYES RED DISCHARGE EYES 498 SKIN & PELAGE COLD TO TOUCH ENTIRE BODY 498 PALE ENTIRE BODY 498 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 197, 204, 211, 218, 225, 232, 239, 246, 253 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 62 WEEK 72 428, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C 498C 1069 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 360 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91278 T 105 EYES CLEAR DISCHARGE EYE-LEFT 568 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 575, 582, 680, 687, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 589, 610, 617, 624, 694, 701, 708, 715, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 631, 638, 645, 652, 722 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 659, 666, 673 MASS(ES) AXILLARY REGION-LEFT WEEK 105 TAIL-PROXIMAL WEEK 86 VENTRAL-CERVICAL-RIGHT WEEK 105 729-730C 596, 603 729-730C C91279 T 105 DISCHARGE ORAL RED IN COLOR 493 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT 568, 575, 582, 589 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729, 731 617, 624, 631 1070 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 361 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91279 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 MASS(ES) AXILLARY REGION-RIGHT WEEK 96 INGUINAL REGION-LEFT WEEK 80 INGUINAL REGION-RIGHT WEEK 86 VENTRAL-ABDOMINAL-LEFT WEEK 53 666, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 731C 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C 708C, 715C, 722C, 729C, 731C 596, 603, 610 365C, 442C, 512C, 589C, 666C, 372C, 449C, 519C, 596C, 673C, 379C, 456C, 526C, 603C, 680C, 386C, 463C, 533C, 610C, 687C, 393C, 470C, 540C, 617C, 694C, 400C, 477C, 547C, 624C, 701C, 407C, 484C, 554C, 631C, 708C, 414C, 491C, 561C, 638C, 715C, 421C, 493C, 568C, 645C, 722C, 428C, 498C, 575C, 652C, 729C, 435C 505C 582C 659C 731C C91280 M 98 APPEARANCE HUNCHED THIN 671, 673, 680-681 681 BEHAVIOR ATAXIC HYPOACTIVE 681 671, 673, 680-681 EXCRETION FEW FECES 671, 673, 680-681 1071 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 362 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91280 M 98 EYES RED DISCHARGE EYES 671, 673, 680-681 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C91281 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C91282 M 53 BEHAVIOR HYPOACTIVE 358, 365 DISCHARGE NASAL RED IN COLOR 351, 356, 358, 365 EXCRETION FEW FECES 358, 365 EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL 356, 358, 365 358, 365 1072 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 363 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91282 M 53 RESPIRATION AUDIBLE 365 LABORED 365 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA 351, 356, 358, 365 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309 WART LIKE LESION(S) VENTRAL-THORACIC WEEK 46 316, 323, 330, 337, 344, 351 C91283 M 93 APPEARANCE HUNCHED THIN 629 617, 624, 629, 631, 638, 645, 647 BEHAVIOR ATAXIC 647 HYPOACTIVE 629 EXCRETION FEW FECES NO FECES 610, 647 617, 629 1073 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 364 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91283 M 93 EYES RED DISCHARGE EYES 629 SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB DORSAL PAW-HIND-RIGHT YELLOW HAIR COAT 647 498, 505 617, 624, 629, 631, 638, 645, 647 617, 624, 629, 631, 638, 645, 647 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 512, 519, 526, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 533, 540, 547, 554, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 561 MASS(ES) AXILLARY REGION-RIGHT WEEK 82 VENTRAL-ABDOMINAL-RIGHT WEEK 82 568, 575, 582, 589C, 596C, 603C, 610C, 617C, 624C, 629C, 631C 638C, 645C, 647C 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 629C, 631C 638C, 645C, 647C 1074 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 365 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91284 D 100 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C91285 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91286 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729-730 C91287 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 1075 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 366 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91288 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C91289 M 73 APPEARANCE HEAD TILT LEFT HUNCHED MALOCCLUSION THIN 484, 491, 498, 505, 508 508 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498 505, 508 491, 498, 505, 508 BEHAVIOR ATAXIC 508 DISCHARGE ORAL CLEAR 508 EXCRETION FEW FECES 484, 491, 498, 505, 508 RESPIRATION AUDIBLE 508 IRREGULAR 508 LABORED 498 SKIN & PELAGE COLD TO TOUCH LIMBS-ALL 508 1076 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 367 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91289 M 73 SKIN & PELAGE YELLOW HAIR COAT 421, 428 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 C91290 M 59 APPEARANCE HEAD TILT RIGHT MALOCCLUSION THIN 393, 400, 407 43, 50, 57, 64, 71, 148, 155, 162, 169, 239, 246, 253, 260, 330, 337, 344, 351, 393, 400, 407 78, 85, 92, 99, 106, 113, 120, 127, 134, 141 176, 183, 190, 197, 204, 211, 218, 225, 232 267, 274, 281, 288, 295, 302, 309, 316, 323 358, 365, 372, 379, 386, 393, 400, 407 SKIN & PELAGE YELLOW HAIR COAT 372 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36 MASS(ES) AXILLARY REGION-RIGHT WEEK 52 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C 1077 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 368 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91291 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91292 T 105 APPEARANCE MALOCCLUSION 351, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708, 715, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 393, 400, 484, 491, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 407, 414, 421, 428, 498, 505 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 358, 365, 435, 442, 449, 456, 92, 99, 106 183, 190, 197 274, 281, 288 372, 379, 386 463, 470, 477 C91293 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C91294 T 105 APPEARANCE MALOCCLUSION 603, 610, 617, 624, 631 EYES CLEAR DISCHARGE EYE-LEFT 498, 505 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 1078 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 369 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91294 T 105 NORMAL NO REMARKABLE OBSERVATIONS 477, 484, 491, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 722, 729, 731 C91295 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 589, 596 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 582 MASS(ES) AXILLARY REGION-LEFT WEEK 81 WEEK 87 VENTRAL-ABDOMINAL-LEFT WEEK 99 561, 568, 575 603, 610, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 732C 687C, 694C, 701C, 708C, 715C, 722C, 729C, 732C C91296 M 86 APPEARANCE HUNCHED THIN 594, 596 582, 587, 589, 594, 596 BEHAVIOR HYPOACTIVE 596 EXCRETION FEW FECES 582, 587, 589, 594 1079 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 370 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91296 M 86 EYES RED DISCHARGE EYES 582, 587, 589, 594, 596 SKIN & PELAGE YELLOW HAIR COAT 594, 596 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C91297 D 91 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) DORSAL WEEK 90 624C, 631C, 636C 1080 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 371 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91298 M 74 APPEARANCE MALOCCLUSION THIN 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505 512 477, 484, 491, 498, 505, 512 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA YELLOW HAIR COAT 400, 407 477, 484, 491, 498, 505, 512 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141 MASS(ES) AXILLARY REGION-LEFT WEEK 43 295, 302, 309C, 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C C91299 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 1081 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 372 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91300 D 83 APPEARANCE THIN 498, 505, 512, 519, 526, 533, 536, 540, 547, 549, 554, 561, 564 568, 570, 575, 579 BEHAVIOR CIRCLING LEFT 549, 554 EXCRETION FEW FECES 570, 575, 579 EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL 536, 540, 547, 549, 554, 561, 564, 568, 570, 575, 579 526, 533 RESPIRATION AUDIBLE 575, 579 SKIN & PELAGE PALE ENTIRE BODY YELLOW HAIR COAT 570 536, 540, 547, 549, 554, 561, 564, 568, 570, 575, 579 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 456, 463, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 470, 477, 484, 491 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 435, 442, 449 1082 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 373 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91300 D 83 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C C91301 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 MASS(ES) AXILLARY REGION-RIGHT WEEK 99 INGUINAL REGION-LEFT WEEK 105 687C, 694C, 701C, 708C, 715C, 722C, 729-730C 729-730C C91302 M 72 APPEARANCE HUNCHED THIN 484, 491, 498, 501, 504 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 501, 504 BEHAVIOR HYPOACTIVE 428, 435, 442, 449, 504 EXCRETION DISCOLORED URINE RED IN COLOR 407 FEW FECES 504 1083 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 374 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91302 M 72 EYES RED DISCHARGE EYES 414, 421, 428, 435, 442, 449 RESPIRATION AUDIBLE 501 SKIN & PELAGE COLD TO TOUCH ENTIRE BODY ROUGH HAIR COAT YELLOW HAIR COAT 504 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 501, 504 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 501, 504 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 400 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 51 351C, 358C, 365C, 372C, 379C, 386C, 393C C91303 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 1084 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 375 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91303 T 105 NORMAL NO REMARKABLE OBSERVATIONS 659, 666, 673, 680, 687 MASS(ES) AXILLARY REGION-RIGHT WEEK 105 VENTRAL-ABDOMINAL-LEFT WEEK 100 729-730 694C, 701C, 708C, 715C, 722C, 729-730C C91304 3 14 SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL-LEFT VENTRAL-CERVICAL-RIGHT 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 NORMAL NO REMARKABLE OBSERVATIONS 1, 8 C91305 M 87 APPEARANCE CONVULSIONS PARALYSIS LIMBS-HIND 288, 295, 302, 309, 316, 372, 379, 386, 400, 407 603-604 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 330, 337, 456, 463, 547, 554, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 344, 351, 358, 365, 470, 477, 484, 491, 561, 568, 575, 582, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 393, 414, 421, 428, 498, 505, 512, 519, 589, 596 92, 99, 106 183, 190, 197 274, 281, 323 435, 442, 449 526, 533, 540 1085 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 376 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91306 M 103 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 463, 470, 477, 484, 491 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 498, 505 MASS(ES) AXILLARY REGION-LEFT WEEK 74 AXILLARY REGION-RIGHT WEEK 85 INGUINAL REGION-LEFT WEEK 81 WEEK 93 INGUINAL REGION-RIGHT WEEK 92 512, 519, 526, 533, 540, 547, 554, 561, 568, 575C, 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C 673C, 680C, 687C, 694C, 701C, 708C, 715C 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C 715C 1086 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 377 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91307 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 731 C91308 M 69 APPEARANCE HEAD TILT RIGHT 470, 477, 480 BEHAVIOR ATAXIC 480 SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL-LEFT 15, 22 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113, 120 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435, 442, 449, 456, 463 1087 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 378 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 30 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91309 M 67 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT 428, 435, 442, 449, 456, 463-464 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 57 VENTRAL-CERVICAL WEEK 62 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C 463-464C 428C, 435C, 442C, 449C, 456C, 463-464C C91310 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) AXILLARY REGION-LEFT WEEK 71 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 722C, 729C, 732C 1088 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 379 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91253 F3 729 105 TO 730 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 105 30 X 30MM C91255 F3 470 68 TO 477 69 MASS(ES) AXILLARY REGION-LEFT WEEK 64 10 X 10MM 484 70 MASS(ES) AXILLARY REGION-LEFT WEEK 64 20 X 20MM 491 71 TO 498 72 MASS(ES) AXILLARY REGION-LEFT WEEK 64 30 X 30MM 505 73 TO 513 74 MASS(ES) AXILLARY REGION-LEFT WEEK 64 10 X 10MM 428 62 TO 463 67 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 62 10 X 10MM 470 68 TO 484 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 62 15 X 20MM 491 71 TO 513 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 62 20 X 30MM 1089 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 380 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91256 F3 645 93 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL WEEK 90 10 X 10MM 666 96 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL WEEK 90 20 X 20MM 687 99 MASS(ES) VENTRAL-ABDOMINAL WEEK 90 30 X 30MM 694 100 TO 731 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 90 40 X 30MM C91258 F3 519 75 TO 533 77 MASS(ES) AXILLARY REGION-LEFT WEEK 75 15 X 15MM 491 71 TO 512 74 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 15 X 15MM 519 75 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 20 X 20MM 526 76 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 20 X 20MM 1090 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 381 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91258 F3 533 77 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 20 X 20MM 540 78 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 20 X 20MM 547 79 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 20 X 20MM 554 80 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 20 X 20MM 561 81 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 20 X 20MM 568 82 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 30 X 30MM 575 83 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 30 X 30MM 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 30 X 45MM 1091 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 382 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91258 F3 589 85 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 45 X 50MM 596 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 45 X 50MM 600 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 45 X 50MM 600 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 45 X 50MM 600 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 45 X 50MM 547 79 TO 561 81 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 79 20 X 20MM 568 82 TO 582 84 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 79 30 X 30MM 589 85 TO 600 86 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 79 40 X 30MM 1092 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 383 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91261 F3 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 30 X 30MM 610 88 TO 638 92 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 40 X 35MM 645 93 TO 659 95 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 50 X 45MM 666 96 TO 689 99 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 60 X 70MM C91263 F3 225 33 TO 253 37 MASS(ES) AXILLARY REGION-LEFT WEEK 33 10 X 10MM 176 26 TO 232 34 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 22 10 X 10MM 239 35 TO 302 44 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 22 30 X 30MM 309 45 TO 330 48 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 22 20 X 20MM 1093 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 384 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91263 F3 337 49 TO 386 56 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 22 30 X 30MM 393 57 TO 421 61 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 22 40 X 35MM 428 62 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 22 40 X 40MM C91264 F3 687 99 MASS(ES) AXILLARY REGION-LEFT WEEK 97 15 X 15MM 694 100 TO 730 105 MASS(ES) AXILLARY REGION-LEFT WEEK 97 25 X 25MM 645 93 TO 680 98 MASS(ES) AXILLARY REGION-RIGHT WEEK 93 10 X 10MM 687 99 MASS(ES) AXILLARY REGION-RIGHT WEEK 93 15 X 15MM 694 100 TO 730 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 93 20 X 20MM 1094 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 385 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91266 F3 694 100 TO 723 104 MASS(ES) AXILLARY REGION-RIGHT WEEK 97 10 X 10MM 617 89 TO 708 102 MASS(ES) INGUINAL REGION-RIGHT WEEK 89 20 X 20MM 713 102 MASS(ES) INGUINAL REGION-RIGHT WEEK 89 20 X 20MM 715 103 MASS(ES) INGUINAL REGION-RIGHT WEEK 89 20 X 20MM 722 104 MASS(ES) INGUINAL REGION-RIGHT WEEK 89 20 X 20MM 723 104 MASS(ES) INGUINAL REGION-RIGHT WEEK 89 20 X 20MM 723 104 MASS(ES) INGUINAL REGION-RIGHT WEEK 89 20 X 20MM 274 40 TO 330 48 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 40 10 X 10MM 1095 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 386 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91267 F3 260 38 TO 281 41 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 38 10 X 10MM 288 42 TO 344 50 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 38 20 X 25MM 351 51 TO 365 53 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 38 25 X 30MM 372 54 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 38 40 X 40MM 379 55 TO 393 57 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 38 20 X 20MM 400 58 TO 435 63 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 38 20 X 35MM 442 64 TO 464 67 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 38 25 X 35MM 316 46 TO 323 47 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 46 10 X 10MM 1096 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 387 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91267 F3 330 48 TO 344 50 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 46 20 X 20MM 351 51 TO 379 55 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 46 30 X 25MM 386 56 TO 407 59 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 46 35 X 30MM 414 60 TO 421 61 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 46 45 X 55MM 428 62 TO 435 63 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 46 60 X 55MM 442 64 TO 464 67 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 46 70 X 70MM C91268 F3 554 80 TO 575 83 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 80 10 X 10MM 582 84 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 80 15 X 20MM 1097 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 388 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91268 F3 589 85 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 80 25 X 25MM 596 86 TO 673 97 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 80 30 X 30MM 673 97 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 80 30 X 40MM C91271 F3 589 85 MASS(ES) AXILLARY REGION-LEFT WEEK 84 15 X 15MM 596 86 MASS(ES) AXILLARY REGION-LEFT WEEK 84 20 X 15 603 87 TO 631 91 MASS(ES) AXILLARY REGION-LEFT WEEK 84 30 X 30MM 638 92 MASS(ES) AXILLARY REGION-LEFT WEEK 84 30 X 40MM 645 93 TO 652 94 MASS(ES) AXILLARY REGION-LEFT WEEK 84 40 X 40MM 1098 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 389 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91271 F3 653 94 TO 653 94 MASS(ES) AXILLARY REGION-LEFT WEEK 84 30 X 30MM 653 94 MASS(ES) AXILLARY REGION-LEFT WEEK 84 30 X 30MM 554 80 TO 575 83 MASS(ES) INGUINAL REGION-LEFT WEEK 75 20 X 20MM 582 84 MASS(ES) INGUINAL REGION-LEFT WEEK 84 20 X 30 NN 589 85 TO 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 84 30 X 45MM 603 87 TO 653 94 MASS(ES) INGUINAL REGION-LEFT WEEK 84 35 X 50MM C91275 F3 414 60 TO 421 61 MASS(ES) AXILLARY REGION-LEFT WEEK 58 20 X 15MM 428 62 MASS(ES) AXILLARY REGION-LEFT WEEK 58 20 X 20MM 1099 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 390 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91275 F3 435 63 TO 480 69 MASS(ES) AXILLARY REGION-LEFT WEEK 58 25 X 25MM 358 52 TO 407 59 MASS(ES) AXILLARY REGION-RIGHT WEEK 52 10 X 10MM C91276 F3 568 82 TO 582 84 MASS(ES) AXILLARY REGION-LEFT WEEK 82 10 X10MM 589 85 TO 603 87 MASS(ES) AXILLARY REGION-LEFT WEEK 82 15 X 15MM 610 88 MASS(ES) AXILLARY REGION-LEFT WEEK 82 20 X 20MM 617 89 TO 673 97 MASS(ES) AXILLARY REGION-LEFT WEEK 82 30 X 20MM 568 82 TO 575 83 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 82 20 X20MM 582 84 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 82 20 X 30MM 1100 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 391 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91276 F3 589 85 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 82 35 X 30MM 596 86 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 82 40 X 35MM 603 87 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 82 45 X 35MM 610 88 TO 638 92 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 82 55 X 45MM 645 93 TO 673 97 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 82 70 X 60MM 610 88 TO 659 95 MASS(ES) INGUINAL REGION-LEFT WEEK 88 20 X 20MM 666 96 TO 673 97 MASS(ES) INGUINAL REGION-LEFT WEEK 88 30 X 20MM 498 72 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL WEEK 72 20 X 20MM 1101 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 392 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91276 F3 589 85 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL WEEK 72 35 X 35MM 603 87 TO 673 97 MASS(ES) VENTRAL-ABDOMINAL WEEK 72 40 X 45MM 484 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 62 10 X 10MM 491 71 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 62 20 X 20MM 589 85 TO 638 92 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 62 20 X 35MM 645 93 TO 673 97 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 62 30 X 40MM C91277 F3 435 63 TO 456 66 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 62 20 X 20MM 463 67 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 62 25MM X 25MM 1102 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 393 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91277 F3 470 68 TO 477 69 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 62 55 X 30MM 484 70 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 62 80 X 50MM 498 72 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 72 30 X 50MM C91278 F3 729 105 TO 730 105 MASS(ES) AXILLARY REGION-LEFT WEEK 105 10 X 10MM 729 105 TO 730 105 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 105 20 X 20MM C91279 F3 673 97 TO 687 99 MASS(ES) AXILLARY REGION-RIGHT WEEK 96 20 X 20MM 694 100 TO 731 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 96 35 X 30MM 554 80 TO 638 92 MASS(ES) INGUINAL REGION-LEFT WEEK 80 20 X 20MM 1103 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 394 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91279 F3 645 93 TO 687 99 MASS(ES) INGUINAL REGION-LEFT WEEK 80 10 X 10MM 694 100 TO 731 105 MASS(ES) INGUINAL REGION-LEFT WEEK 80 20 X 20MM 365 53 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 53 20 X 30MM 372 54 TO 407 59 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 53 30 X 20MM 414 60 TO 477 69 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 53 15 X 15MM 484 70 TO 512 74 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 53 30 X 20MM 519 75 TO 561 81 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 53 35 X 30MM 568 82 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 53 40 X 40MM 1104 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 395 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91279 F3 589 85 TO 687 99 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 53 50 X 50MM 694 100 TO 708 102 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 53 40 X 70MM 715 103 TO 731 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 53 70 X 70MM C91283 F3 589 85 TO 596 86 MASS(ES) AXILLARY REGION-RIGHT WEEK 82 15 X 15MM 603 87 TO 647 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 82 20 X 20MM 568 82 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 82 20 X 20MM 589 85 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 82 30 X 30MM 603 87 TO 647 93 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 82 35 X 30MM 1105 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 396 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91290 F3 358 52 TO 407 59 MASS(ES) AXILLARY REGION-RIGHT WEEK 52 10 X 10MM C91295 F3 617 89 TO 687 99 MASS(ES) AXILLARY REGION-LEFT WEEK 87 20 X 25MM 694 100 TO 732 105 MASS(ES) AXILLARY REGION-LEFT WEEK 87 30 X 30MM 687 99 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 99 40 X 40MM 694 100 TO 732 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 99 50 X 55MM C91297 F3 624 90 TO 636 91 MASS(ES) DORSAL WEEK 90 50 X 40 C91298 F3 309 45 TO 330 48 MASS(ES) AXILLARY REGION-LEFT WEEK 43 15 X 15MM 337 49 TO 344 50 MASS(ES) AXILLARY REGION-LEFT WEEK 43 20 X 20MM 1106 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 397 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91298 F3 351 51 TO 372 54 MASS(ES) AXILLARY REGION-LEFT WEEK 43 30 X 25MM 379 55 TO 393 57 MASS(ES) AXILLARY REGION-LEFT WEEK 43 35 X 25MM 400 58 TO 407 59 MASS(ES) AXILLARY REGION-LEFT WEEK 43 40 X 35MM 414 60 MASS(ES) AXILLARY REGION-LEFT WEEK 43 55 X 45MM 421 61 TO 512 74 MASS(ES) AXILLARY REGION-LEFT WEEK 43 55 X 55MM C91300 F3 365 53 TO 379 55 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 10 X 10MM 386 56 TO 428 62 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 20 X 15MM C91301 F3 687 99 MASS(ES) AXILLARY REGION-RIGHT WEEK 99 15 X 15MM 1107 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 398 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91301 F3 694 100 TO 730 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 99 30 X 30MM 729 105 TO 730 105 MASS(ES) INGUINAL REGION-LEFT WEEK 105 20 X 20MM C91302 F3 351 51 TO 393 57 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 51 10 X 10MM C91303 F3 694 100 TO 730 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 100 10 X 10MM C91306 F3 575 83 TO 589 85 MASS(ES) AXILLARY REGION-LEFT WEEK 74 20 X 20MM 596 86 TO 659 95 MASS(ES) AXILLARY REGION-LEFT WEEK 74 30 X 25MM 666 96 MASS(ES) AXILLARY REGION-LEFT WEEK 74 30 X 50MM 673 97 TO 687 99 MASS(ES) AXILLARY REGION-LEFT WEEK 74 40 X 50MM 1108 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 399 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91306 F3 694 100 TO 715 103 MASS(ES) AXILLARY REGION-LEFT WEEK 74 60 X 50MM 589 85 MASS(ES) AXILLARY REGION-RIGHT WEEK 85 15 X 10 MM 596 86 TO 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 85 20 X 15MM 610 88 TO 680 98 MASS(ES) AXILLARY REGION-RIGHT WEEK 85 30 X 20MM 687 99 TO 715 103 MASS(ES) AXILLARY REGION-RIGHT WEEK 85 10 X 10MM 561 81 TO 568 82 MASS(ES) INGUINAL REGION-LEFT WEEK 81 20 X 20MM 575 83 MASS(ES) INGUINAL REGION-LEFT WEEK 81 40 X 40MM 582 84 TO 589 85 MASS(ES) INGUINAL REGION-LEFT WEEK 81 20X 20MM 1109 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 400 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91306 F3 596 86 TO 610 88 MASS(ES) INGUINAL REGION-LEFT WEEK 81 25 X 20MM 617 89 TO 631 91 MASS(ES) INGUINAL REGION-LEFT WEEK 81 25 X 25MM 645 93 TO 687 99 MASS(ES) INGUINAL REGION-LEFT WEEK 93 10 X 10MM 694 100 TO 715 103 MASS(ES) INGUINAL REGION-LEFT WEEK 93 20 X 20MM 638 92 TO 687 99 MASS(ES) INGUINAL REGION-RIGHT WEEK 92 30 X 20MM 694 100 TO 715 103 MASS(ES) INGUINAL REGION-RIGHT WEEK 92 35 X 25MM C91309 F3 393 57 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 57 15 X 15MM 400 58 TO 407 59 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 57 25 X 25MM 1110 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 401 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91309 F3 414 60 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 57 40 X 40MM 421 61 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 57 45 X 50MM 428 62 TO 464 67 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 57 40 X 45MM 428 62 MASS(ES) VENTRAL-CERVICAL WEEK 62 25 X 25MM 435 63 TO 464 67 MASS(ES) VENTRAL-CERVICAL WEEK 62 30 X 30MM C91310 F3 491 71 TO 498 72 MASS(ES) AXILLARY REGION-LEFT WEEK 71 10 X 10MM 505 73 TO 561 81 MASS(ES) AXILLARY REGION-LEFT WEEK 71 20 X 20MM 568 82 TO 582 84 MASS(ES) AXILLARY REGION-LEFT WEEK 71 40 X 30MM 1111 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 402 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91310 F3 589 85 TO 596 86 MASS(ES) AXILLARY REGION-LEFT WEEK 71 50 X 50MM 603 87 MASS(ES) AXILLARY REGION-LEFT WEEK 71 75 X 50MM 610 88 TO 631 91 MASS(ES) AXILLARY REGION-LEFT WEEK 71 80 X 75MM 638 92 MASS(ES) AXILLARY REGION-LEFT WEEK 71 80 X 65MM 645 93 TO 680 98 MASS(ES) AXILLARY REGION-LEFT WEEK 71 80 X 75MM 687 99 MASS(ES) AXILLARY REGION-LEFT WEEK 71 80 X 80MM 694 100 TO 722 104 MASS(ES) AXILLARY REGION-LEFT WEEK 71 80 X 110MM 729 105 TO 732 105 MASS(ES) AXILLARY REGION-LEFT WEEK 71 80 X 95MM 1112 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 403 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91311 D 64 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 444 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 C91312 M 97 APPEARANCE THIN 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 652, 659, 666, 673, 675 DISCHARGE GENITAL RED IN COLOR 675 EYES PALE EYES 526, 533, 540, 547, 554, 561, 568 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 638, 645 1113 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 404 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91313 M 87 APPEARANCE HEAD TILT RIGHT 608 BEHAVIOR HYPOACTIVE 608 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL VENTRAL-THORACIC 582, 589, 596, 603, 607-608 582, 589, 596, 603, 607-608 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) AXILLARY REGION-LEFT WEEK 74 AXILLARY REGION-RIGHT WEEK 85 VENTRAL-ABDOMINAL WEEK 85 VENTRAL-ABDOMINAL-LEFT WEEK 71 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 603, 607-608 589C, 596C, 603C, 607-608C 589C, 596C, 603C, 607-608C 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C 568C, 575C, 582C, 589C, 596C, 603C, 607-608C 1114 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 405 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91314 M 74 APPEARANCE MALOCCLUSION THIN 498, 505, 512 512 SKIN & PELAGE ALOPECIA RED HAIR COAT VENTRAL-ABDOMINAL ROUGH HAIR COAT SORE/SCAB DORSAL 99, 302, 309 512 358, 365, 372 120, 134 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 106, 113 127, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 316, 323 379, 386, 393, 400, 407, 414 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 48 VENTRAL-CERVICAL-RIGHT WEEK 61 330C, 337C, 344C, 351C, 358C, 365C, 372C 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C 498C, 505C, 512C 1115 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 406 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91315 T 105 EYES RED DISCHARGE EYE-RIGHT 400 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 484, 491, 575, 582, 666, 673, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 407, 414, 421, 428, 498, 505, 512, 519, 589, 596, 603, 610, 680 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 435, 442, 449, 456, 526, 533, 540, 547, 617, 624, 631, 638, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 463, 470, 477 554, 561, 568 645, 652, 659 MASS(ES) DORSAL WEEK 99 WEEK 103 687C, 694C, 701C, 708C, 715C, 722C, 729C, 731C 715C, 722C, 729C, 731C C91316 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91317 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 1116 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 407 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91318 A 15 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 102 C91319 M 70 BEHAVIOR RECUMBENT LATERAL 486 DISCHARGE ORAL CLEAR 486 EYES RED DISCHARGE EYES 486 RESPIRATION IRREGULAR 486 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 1117 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 408 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91320 M 79 APPEARANCE THIN 540, 547 BEHAVIOR ATAXIC 547 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 547 YELLOW HAIR COAT 547 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C91321 M 94 EYES PALE EYES 652, 654 SKIN & PELAGE SORE/SCAB LUMBAR-LEFT 652, 654 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 1118 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 409 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91321 M 94 NORMAL NO REMARKABLE OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617 MASS(ES) DORSAL WEEK 90 624C, 631C, 638C, 645C, 652C, 654C C91322 M 22 APPEARANCE HUNCHED MALOCCLUSION THIN 150 148, 150-151 148, 150-151 DISCHARGE NASAL RED IN COLOR 148, 150-151 EXCRETION NO FECES 150-151 RESPIRATION AUDIBLE 150-151 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL 148, 150-151 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141 1119 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 410 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91323 4 53 SKIN & PELAGE ROUGH HAIR COAT 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 51 351C, 358C, 365C, 367C C91324 T 105 APPEARANCE MALOCCLUSION 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505, 512 EYES RED DISCHARGE EYE-LEFT 533 SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL SORE/SCAB PAW-HIND-RIGHT 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694, 701, 708, 715, 722 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 1120 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 411 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91324 T 105 NORMAL NO REMARKABLE OBSERVATIONS 386, 393, 519, 526, 540, 547, 554, 561, 568, 575 C91325 M 103 APPEARANCE HUNCHED THIN 712, 715 701, 708, 712, 715 BEHAVIOR HYPOACTIVE 701, 708, 712, 715 EXCRETION FEW FECES 715 EYES RED DISCHARGE EYES 712, 715 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA RED HAIR COAT HEAD-CRANIAL 712, 715 652, 659, 666, 673, 680, 687, 694, 701, 708, 712, 715 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645 1121 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 412 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91326 M 86 APPEARANCE HUNCHED THIN 582, 589, 596 540, 547, 554, 561, 568, 575, 579, 582, 589, 596 BEHAVIOR HYPOACTIVE 596 EXCRETION FEW FECES 561, 568, 575, 579, 582, 589, 596 EYES RED DISCHARGE EYES 579, 582 SKIN & PELAGE RED HAIR COAT LIMBS-FRONT YELLOW HAIR COAT 575, 579, 582, 589, 596 575, 579, 582, 589, 596 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 1122 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 413 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91327 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 84 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C 731C C91328 M 63 APPEARANCE MALOCCLUSION 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 437 DISCHARGE ORAL RED IN COLOR 437 EYES PALE EYES 437 RED DISCHARGE EYES 437 1123 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 414 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91328 M 63 RESPIRATION AUDIBLE 437 LABORED 437 SKIN & PELAGE RED HAIR COAT DORSAL-CERVICAL-RIGHT 437 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 C91329 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C91330 M 85 SKIN & PELAGE SORE/SCAB SACRAL-LEFT 582, 589-590 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 MASS(ES) DORSAL WEEK 68 DORSAL-CERVICAL-LEFT WEEK 80 VENTRAL-ABDOMINAL-LEFT WEEK 84 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C 547C, 554C, 561C, 568C, 575C, 582C, 589-590C 554C, 561C, 568C, 575C, 582C, 589-590C 582, 589-590C 1124 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 415 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91330 M 85 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 85 589-590C VENTRAL-ABDOMINAL-RIGHT WEEK 85 589-590C VENTRAL-THORACIC WEEK 54 372, 379, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589-590C C91331 M 78 APPEARANCE THIN 526, 533, 540-541 EXCRETION FEW FECES 523, 526, 533, 540-541 EYES SQUINTED PERI-ORBITAL 541 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL YELLOW HAIR COAT 523, 526, 533, 540 541 523, 526, 533, 540-541 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 1125 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 416 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91331 M 78 NORMAL NO REMARKABLE OBSERVATIONS 477, 484, 491, 498, 505, 512, 519 C91332 T 105 APPEARANCE THIN 582, 589, 596, 603, 610, 701, 708, 715, 722, 729-730 BEHAVIOR HYPOACTIVE 701, 708, 715, 722 EXCRETION FEW FECES 582, 589 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB DORSAL LATERAL-CERVICAL-LEFT LATERAL-CERVICAL-RIGHT 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708, 715, 722, 729-730 8, 15, 22, 29 8, 15 NORMAL NO REMARKABLE OBSERVATIONS 1, 36, 43, 50, 134, 141, 148, 225, 232, 239, 316, 323, 330, 575 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106, 113, 120, 127 183, 190, 197, 204, 211, 218 274, 281, 288, 295, 302, 309 365, 540, 547, 554, 561, 568 1126 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 417 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91333 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91334 D 101 APPEARANCE MALOCCLUSION THIN 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 676, 680, 687, 691, 694, 701, 703, 705 673, 676, 680, 687, 691, 694, 701, 703, 705 BEHAVIOR HYPOACTIVE 701, 703, 705 EXCRETION FEW FECES 676, 691, 703, 705 SKIN & PELAGE SCALY SKIN TAIL-DISTAL 36, 43, 50, 57, 64, 71, 78, 85, 92, 99 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 106, 113, 120, 127, 134, 141, 148, 155, 162 169, 176, 183, 190, 197, 204, 211, 218, 232, 239, 246, 253, 260 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358, 365, 372, 379, 386, 393, 400 MASS(ES) AXILLARY REGION-LEFT WEEK 86 VENTRAL-ABDOMINAL WEEK 33 596, 603, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C 673C, 676C, 680C, 687C, 691C, 694C, 701C, 703C, 705C 225C 1127 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 418 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91334 D 101 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 97 673C, 676C, 680C, 687C, 691C, 694C, 701C, 703C, 705C C91335 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729-730 C91336 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 88 610, 617, 624, 631, 638C, 645C, 652C, 659C, 666C, 673C, 680C 687C, 694C, 701C, 708C, 715C, 722C, 729-730C 1128 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 419 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91337 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C91338 T 105 EYES RED DISCHARGE EYE-LEFT 652, 659, 666, 673, 680, 687, 694, 701 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 715, 722, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 729, 731 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 708 C91339 M 98 APPEARANCE THIN 657, 659, 666, 673, 676, 680 DISCHARGE NASAL RED IN COLOR 657, 659, 666, 673, 676, 680 EXCRETION FEW FECES NO FECES 652, 680 657, 659 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL 652 657, 659, 666, 673, 676, 680 1129 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 420 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91339 M 98 SKIN & PELAGE YELLOW HAIR COAT 652, 657, 659, 666, 673, 676, 680 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645 C91340 T 105 APPEARANCE MALOCCLUSION THIN 99, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 687, 691, 694, 701, 708, 715, 722 729, 731 652, 659, 666, 673, 680, 687, 691, 694, 701, 708, 715, 722, 729 731 BEHAVIOR HYPOACTIVE 701, 708, 715, 722, 729, 731 EXCRETION FEW FECES 691, 694, 701, 708, 715, 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92 1130 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 421 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91340 T 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652C, 659C, 666C, 673C, 680C, 687C, 691C, 694C, 701C, 708C, 715C 722C, 729C, 731C VENTRAL-THORACIC-LEFT WEEK 67 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C 540C, 547C, 554C, 561C, 568C C91341 T 105 APPEARANCE CONVULSIONS 498, 554, 561, 568, 575, 589, 596, 603, 659, 666, 673, 687, 694 701 SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) VENTRAL-CERVICAL WEEK 73 WEEK 75 505, 512, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 729-730C 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C 1131 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 422 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91341 T 105 MASS(ES) VENTRAL-CERVICAL WEEK 75 596C, 603C, 610C C91342 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 731 C91343 4 53 APPEARANCE MALOCCLUSION 302 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C91344 M 103 APPEARANCE HEAD TILT LEFT HUNCHED MALOCCLUSION THIN 713, 715 715 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 713, 715 713, 715 1132 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 423 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91344 M 103 BEHAVIOR HYPOACTIVE 715 EXCRETION FEW FECES 713, 715 EYES SQUINTED PERI-ORBITAL 713, 715 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA 713, 715 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 94 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 713C, 715C C91345 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 1133 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 424 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91346 T 72 APPEARANCE HUNCHED THIN 500-502 500-502 BEHAVIOR HYPOACTIVE 491, 498, 500-502 EXCRETION FEW FECES 491, 498, 500-502 EYES CLEAR DISCHARGE EYES 500-502 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 500-502 484, 491, 498, 500-502 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 1134 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 425 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91347 4 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C91348 4 53 APPEARANCE MALOCCLUSION 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295 C91349 D 59 APPEARANCE MALOCCLUSION THIN 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 410 372 DISCHARGE ORAL RED IN NASAL RED IN COLOR COLOR 268, 271 268, 271 EYES RED DISCHARGE EYES 268, 271, 274, 281, 288, 295 RESPIRATION AUDIBLE 268, 271, 274 1135 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 426 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91349 D 59 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 358, 365, 372 PAWS-HIND 253, 260, 267-268, 271, 274, 281, 288, 295, 302, 309, 316, 323 YELLOW HAIR COAT 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 410 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 120, 127, 134, 141, 148, 176, 183, 190, 197, 204, 211, 218, 225 232, 239, 246 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 17 113 WART LIKE LESION(S) MOUTH WEEK 23 155, 162, 169 C91350 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91351 M 93 BEHAVIOR HYPOACTIVE 645 RESPIRATION LABORED 645 SKIN & PELAGE PALE ENTIRE BODY 645 1136 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 427 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91351 M 93 SKIN & PELAGE SORE/SCAB LATERAL-THORACIC-RIGHT 645 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C C91352 4 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C91353 4 53 APPEARANCE SWOLLEN PAW-HIND-LEFT 225, 232, 239, 246, 253 SKIN & PELAGE ALOPECIA SORE/SCAB MOUTH VENTRAL-CERVICAL 29, 36, 43, 99 225, 232 141, 148 1137 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 428 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91353 4 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 50, 57, 64, 71, 78, 85, 92, 106, 113, 120, 127 134, 155, 162, 169, 176, 183, 190, 197, 204, 260, 267, 274, 281 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 WART LIKE LESION(S) MOUTH WEEK 31 211, 218 C91354 M 88 APPEARANCE HUNCHED THIN 610 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 554, 561, 568, 575, 582, 589, 596, 603, 610 BEHAVIOR HYPOACTIVE 596, 603, 610 EXCRETION FEW FECES 526, 533, 540, 547, 596 RESPIRATION AUDIBLE 526 SKIN & PELAGE RED HAIR COAT LIMBS-FRONT YELLOW HAIR COAT 596, 603, 610 610 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 1138 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 429 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91354 M 88 NORMAL NO REMARKABLE OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 C91355 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C91356 T 105 APPEARANCE MALOCCLUSION THIN 206, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 729, 732 DISCHARGE NASAL RED IN COLOR 206 EXCRETION FEW FECES 211 RESPIRATION AUDIBLE 206 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204 1139 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 430 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91357 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729-730 C91358 M 75 BEHAVIOR HYPOACTIVE 523 EXCRETION FEW FECES 523 EYES RED DISCHARGE EYES 523 SKIN & PELAGE COLD TO TOUCH ENTIRE BODY YELLOW HAIR COAT 523 519, 523 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 1140 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 431 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91358 M 75 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 61 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C 498C, 505C, 512C, 519C, 523C C91359 D 78 APPEARANCE MALOCCLUSION 211, 218 BEHAVIOR SENSITIVE TO TOUCH 260 DISCHARGE GENITAL RED IN COLOR 239, 253, 260 SKIN & PELAGE PALE ENTIRE BODY 260 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 225, 330, 337, 421, 428, 512, 519, 22, 29, 36, 43, 50, 127, 134, 141, 148, 232, 246, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 546 57, 64, 71, 78, 85, 155, 162, 169, 176, 281, 288, 295, 302, 372, 379, 386, 393, 463, 470, 477, 484, 92, 99, 106 183, 190, 197 309, 316, 323 400, 407, 414 491, 498, 505 1141 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 432 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91360 T 105 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 C91361 T 105 APPEARANCE MALOCCLUSION 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 1142 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 433 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91362 T 105 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708, 715, 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) AXILLARY REGION-LEFT WEEK 85 AXILLARY REGION-RIGHT WEEK 89 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 732C 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C 694C, 701C, 708C, 715C, 722C, 729C, 732C C91363 M 97 APPEARANCE MALOCCLUSION THIN 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645 652, 659, 663, 666, 673 673 BEHAVIOR HYPOACTIVE 663, 666, 673 1143 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 434 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91363 M 97 DISCHARGE NASAL RED IN COLOR 663 RESPIRATION AUDIBLE 673 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL RED HAIR COAT HEAD-ENTIRE 663, 666, 673 663, 666, 673 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 414, 421, 512, 519, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 358, 428, 435, 442, 449, 526, 533, 540, 547, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 365, 372, 379, 386, 456, 463, 470, 477, 554 92, 99, 106 183, 190, 197 274, 281, 288 393, 400, 407 491, 498, 505 MASS(ES) AXILLARY REGION-RIGHT WEEK 48 MOUTH WEEK 70 330, 337, 344, 351 484 1144 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 435 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91364 D 101 APPEARANCE THIN 645, 652, 659, 666, 673, 680, 687, 694, 701, 707 DISCHARGE NASAL RED IN COLOR 645, 652, 659, 666, 673, 680, 687, 694, 701, 707 EXCRETION FEW FECES 694, 701, 707 SKIN & PELAGE YELLOW HAIR COAT 694, 701, 707 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638 C91365 4 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 1145 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 436 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91366 M 86 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 596, 600 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) INGUINAL REGION-LEFT WEEK 80 VENTRAL-ABDOMINAL-LEFT WEEK 74 554C, 561C, 568C, 575C, 582C, 589C, 596C, 600C 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C 589C, 596C, 600C C91367 4 53 APPEARANCE MALOCCLUSION 229, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309 316, 323, 330, 337, 344, 351, 358, 365, 367 SKIN & PELAGE SORE/SCAB MOUTH 229, 232 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225 1146 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 437 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91368 4 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C91369 M 74 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302 MASS(ES) AXILLARY REGION-LEFT WEEK 63 AXILLARY REGION-RIGHT WEEK 45 VENTRAL-THORACIC-LEFT WEEK 67 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C 512C 309, 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C, 372C, 379C 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C 463, 470, 477, 484C, 491C, 498C, 505C, 512C C91370 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 1147 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 438 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91371 4 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351 MASS(ES) AXILLARY REGION-RIGHT WEEK 52 358, 365, 367 C91372 T 105 APPEARANCE MALOCCLUSION 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 DISCHARGE NASAL RED IN COLOR 218 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211 1148 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 439 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91373 M 69 APPEARANCE HUNCHED 477 THIN 477 BEHAVIOR HYPOACTIVE 477 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL YELLOW HAIR COAT 470, 477 477 477 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463 C91374 M 98 APPEARANCE MALOCCLUSION THIN 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505 512, 519, 526, 533, 540, 547, 554, 561, 568 677, 680 DISCHARGE NASAL RED IN COLOR 673, 677, 680 EXCRETION FEW FECES 677 NO FECES 680 1149 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 440 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91374 M 98 EYES RED DISCHARGE EYE-RIGHT 673, 677, 680 SKIN & PELAGE YELLOW HAIR COAT 677, 680 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 575, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 582, 589, 596, 603 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 WART LIKE LESION(S) TAIL-PROXIMAL WEEK 88 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 677, 680 C91375 M 83 APPEARANCE HUNCHED THIN 578-579 578-579 BEHAVIOR HYPOACTIVE 579 EXCRETION FEW FECES 575, 578-579 EYES RED DISCHARGE EYES 575, 578-579 1150 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 441 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91375 M 83 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568 C91376 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) AXILLARY REGION-LEFT WEEK 100 INGUINAL REGION-LEFT WEEK 105 694C, 701C, 708C, 715C, 722C, 729-730C 729-730C WART LIKE LESION(S) DORSAL WEEK 89 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 722 1151 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 442 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91377 D 71 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 43 295C, 302C, 309C, 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C 449C, 456C, 463C, 470C, 477C, 484C, 491C, 497C C91378 T 105 APPEARANCE MALOCCLUSION THIN 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617 624, 631, 638, 645, 652, 659, 663 663, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 DISCHARGE NASAL RED IN COLOR 663, 666, 673, 680 EXCRETION FEW FECES NO FECES 666, 673, 680 663 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL 663, 666, 673, 680 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 1152 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 443 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91378 T 105 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344 C91379 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 C91380 T 105 APPEARANCE CONVULSIONS MALOCCLUSION 498, 505 253, 260, 267, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694, 701, 708, 715, 719, 722, 729-730 DISCHARGE GENITAL MUCOID 372, 379, 386 EYES DISCOLORED/LIGHT RED EYE-RIGHT 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 719, 722 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 274, 281, 288, 295, 302, 309 316, 323 1153 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 444 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91380 T 105 MASS(ES) AXILLARY REGION-LEFT WEEK 92 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C 715C, 719C, 722C, 729-730C VENTRAL-ABDOMINAL-RIGHT WEEK 100 694C, 701C, 708C, 715C, 719C, 722C, 729-730C 1154 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 445 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91313 F4 589 85 TO 608 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 85 25 X 30MM 589 85 TO 608 87 MASS(ES) VENTRAL-ABDOMINAL WEEK 85 45 X 45MM 491 71 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 20 X 20MM 547 79 TO 568 82 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 25 X 25MM 575 83 TO 589 85 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 30 X 25MM 596 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 30 X 25MM 603 87 TO 608 87 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 40 X 40MM C91314 F4 330 48 TO 372 54 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 48 10 X 15MM 1155 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 446 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91314 F4 421 61 TO 512 74 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 61 35 X 35MM C91315 F4 687 99 TO 731 105 MASS(ES) DORSAL WEEK 99 10 X 10MM 715 103 MASS(ES) DORSAL WEEK 103 30 X 30MM SIDE RIGHT 722 104 TO 731 105 MASS(ES) DORSAL WEEK 103 50 X 40MM SIDE RIGHT C91321 F4 624 90 TO 631 91 MASS(ES) DORSAL WEEK 90 30 X 30 638 92 TO 645 93 MASS(ES) DORSAL WEEK 90 40 X 45MM 652 94 TO 654 94 MASS(ES) DORSAL WEEK 90 20 X 20MM C91323 F4 351 51 TO 367 53 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 51 10 X 10MM 1156 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 447 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91327 F4 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 84 10 X 10MM 589 85 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 84 15 X 15MM 638 92 TO 652 94 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 84 15 X 25MM 659 95 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 84 20 X 25MM 666 96 TO 673 97 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 84 30 X 25MM 680 98 TO 722 104 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 84 30 X 30MM 729 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 84 30 X 30MM 731 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 84 30 X 30MM 1157 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 448 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91330 F4 470 68 TO 477 69 MASS(ES) DORSAL WEEK 68 10 X 10MM 484 70 TO 512 74 MASS(ES) DORSAL WEEK 68 20 X 20MM 519 75 TO 582 84 MASS(ES) DORSAL WEEK 68 25 X 25MM 589 85 TO 590 85 MASS(ES) DORSAL WEEK 68 35 X 35MM 554 80 TO 568 82 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 80 20 X 20MM 575 83 TO 590 85 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 80 30 X 30MM 589 85 TO 590 85 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 84 15 X 15MM ANTERIOR 589 85 TO 590 85 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 85 20 X 20MM POSTERIOR 1158 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 449 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91330 F4 589 85 TO 590 85 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 85 20 X 20 MM 386 56 MASS(ES) VENTRAL-THORACIC WEEK 54 10 X 10MM 393 57 TO 407 59 MASS(ES) VENTRAL-THORACIC WEEK 54 25 X 25MM 414 60 MASS(ES) VENTRAL-THORACIC WEEK 54 30 X 25MM 421 61 MASS(ES) VENTRAL-THORACIC WEEK 54 40 X 30MM 428 62 TO 435 63 MASS(ES) VENTRAL-THORACIC WEEK 54 40 X 35MM 442 64 TO 512 74 MASS(ES) VENTRAL-THORACIC WEEK 54 45 X 40MM 519 75 TO 590 85 MASS(ES) VENTRAL-THORACIC WEEK 54 50 X 45MM 1159 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 450 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91334 F4 610 88 TO 631 91 MASS(ES) AXILLARY REGION-LEFT WEEK 86 10 X 10MM 638 92 TO 666 96 MASS(ES) AXILLARY REGION-LEFT WEEK 86 20 X 20MM 673 97 TO 676 97 MASS(ES) AXILLARY REGION-LEFT WEEK 86 25 X 25MM 680 98 TO 705 101 MASS(ES) AXILLARY REGION-LEFT WEEK 86 30 X 30MM 225 33 MASS(ES) VENTRAL-ABDOMINAL WEEK 33 10 X 10MM 673 97 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 97 45 X 60MM 687 99 TO 705 101 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 97 60 X 60MM C91336 F4 638 92 TO 666 96 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 88 10 X 10MM 1160 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 451 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91336 F4 673 97 TO 730 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 88 10 X 15MM C91340 F4 575 83 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 50 X 40MM APPEARS TO HAVE MIGRATED FROM VENTRAL THORACIC 589 85 TO 731 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 60 X 55MM APPEARS TO HAVE MIGRATED FROM VENTRAL THORACIC 463 67 TO 484 70 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 67 40 X 20MM 491 71 TO 540 78 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 67 40 X 30MM 547 79 TO 568 82 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 67 50 X 40MM C91341 F4 519 75 TO 540 78 MASS(ES) VENTRAL-CERVICAL WEEK 73 15 X 15MM POSTERIOR 547 79 TO 568 82 MASS(ES) VENTRAL-CERVICAL WEEK 73 30 X 30MM POSTERIOR 1161 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 452 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91341 F4 575 83 MASS(ES) VENTRAL-CERVICAL WEEK 73 40 X 40MM POSTERIOR 582 84 MASS(ES) VENTRAL-CERVICAL WEEK 73 45 X 45MM POSTERIOR 589 85 TO 610 88 MASS(ES) VENTRAL-CERVICAL WEEK 73 55 X 55MM POSTERIOR 617 89 TO 631 91 MASS(ES) VENTRAL-CERVICAL WEEK 73 50 X 40MM 638 92 TO 666 96 MASS(ES) VENTRAL-CERVICAL WEEK 73 55 X 40MM 673 97 TO 730 105 MASS(ES) VENTRAL-CERVICAL WEEK 73 60 X 40MM 519 75 TO 568 82 MASS(ES) VENTRAL-CERVICAL WEEK 75 ANTERIOR 575 83 TO 582 84 MASS(ES) VENTRAL-CERVICAL WEEK 75 10 X 10MM ANTERIOR 1162 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 453 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91341 F4 589 85 TO 610 88 MASS(ES) VENTRAL-CERVICAL WEEK 75 15 X 15MM ANTERIOR C91344 F4 652 94 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 94 30 X 30MM 659 95 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 94 30 X 40MM 666 96 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 94 40 X 50MM 673 97 TO 715 103 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 94 60 X 50MM C91351 F4 582 84 TO 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 10 X 10MM 610 88 TO 631 91 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 15 X 15MM 638 92 TO 645 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 30 X 30MM 1163 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 454 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91351 F4 645 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 15 X 15MM 645 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 15 X 15MM C91358 F4 421 61 TO 449 65 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 61 30 X 35MM 456 66 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 61 50 X 35MM 463 67 TO 523 75 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 61 50 X 40MM C91362 F4 589 85 TO 603 87 MASS(ES) AXILLARY REGION-LEFT WEEK 85 30 X 25MM 610 88 TO 631 91 MASS(ES) AXILLARY REGION-LEFT WEEK 85 40 X 35MM 638 92 TO 666 96 MASS(ES) AXILLARY REGION-LEFT WEEK 85 60 X 50MM 1164 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 455 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91362 F4 673 97 TO 722 104 MASS(ES) AXILLARY REGION-LEFT WEEK 85 70 X 70MM 729 105 TO 732 105 MASS(ES) AXILLARY REGION-LEFT WEEK 85 90 X 65MM 617 89 TO 631 91 MASS(ES) AXILLARY REGION-RIGHT WEEK 89 10 X 10MM 638 92 TO 645 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 89 15 X 20MM 652 94 TO 673 97 MASS(ES) AXILLARY REGION-RIGHT WEEK 89 40 X 30MM 680 98 TO 732 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 89 50 X 40MM C91366 F4 554 80 TO 568 82 MASS(ES) INGUINAL REGION-LEFT WEEK 80 10 X 10MM 575 83 MASS(ES) INGUINAL REGION-LEFT WEEK 80 20 X 20MM 1165 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 456 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91366 F4 582 84 TO 600 86 MASS(ES) INGUINAL REGION-LEFT WEEK 80 35 X 30MM 512 74 TO 519 75 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 74 15 X 15MM 526 76 TO 547 79 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 74 20 X 25MM 554 80 TO 568 82 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 74 40 X 30MM 575 83 TO 600 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 74 30 X 30MM C91369 F4 435 63 TO 477 69 MASS(ES) AXILLARY REGION-LEFT WEEK 63 20 X 20MM 484 70 TO 512 74 MASS(ES) AXILLARY REGION-LEFT WEEK 63 20 X 25MM 316 46 TO 323 47 MASS(ES) AXILLARY REGION-RIGHT WEEK 45 20 X 25MM 1166 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 457 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91369 F4 330 48 TO 365 53 MASS(ES) AXILLARY REGION-RIGHT WEEK 45 35 X 30MM 372 54 TO 400 58 MASS(ES) AXILLARY REGION-RIGHT WEEK 45 35 X 40MM 407 59 MASS(ES) AXILLARY REGION-RIGHT WEEK 45 40 X 40MM 414 60 MASS(ES) AXILLARY REGION-RIGHT WEEK 45 45 X 45MM 421 61 MASS(ES) AXILLARY REGION-RIGHT WEEK 45 45 X 50MM 428 62 TO 477 69 MASS(ES) AXILLARY REGION-RIGHT WEEK 45 65 X 60MM 484 70 TO 491 71 MASS(ES) AXILLARY REGION-RIGHT WEEK 45 65 X 65MM 498 72 TO 512 74 MASS(ES) AXILLARY REGION-RIGHT WEEK 45 55 X 55MM 1167 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 458 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91369 F4 484 70 TO 512 74 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 67 20 X 20MM C91376 F4 694 100 TO 730 105 MASS(ES) AXILLARY REGION-LEFT WEEK 100 10 X 10MM 729 105 TO 730 105 MASS(ES) INGUINAL REGION-LEFT WEEK 105 THREE MASSES UNDER 10MM C91377 F4 295 43 TO 302 44 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 43 10 X 10MM 309 45 TO 344 50 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 43 25 X 20MM 351 51 TO 365 53 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 43 40 X 30MM 372 54 TO 400 58 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 43 40 X 50MM 407 59 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 43 55 X 55MM 1168 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 459 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91377 F4 414 60 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 43 60 X 60MM 421 61 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 43 70 X 65MM 428 62 TO 497 71 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 43 90 X 85MM C91380 F4 638 92 TO 666 96 MASS(ES) AXILLARY REGION-LEFT WEEK 92 25 X 30MM 673 97 TO 730 105 MASS(ES) AXILLARY REGION-LEFT WEEK 92 35 X 30MM 694 100 TO 730 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 100 35 X 35MM 1169 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 460 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91381 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91382 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91383 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91384 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91385 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91386 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91387 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91388 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91389 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1170 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 461 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91390 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91391 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91392 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91393 2 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 54 C91394 2 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 54 C91395 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91396 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91397 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91398 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1171 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 462 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91399 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91400 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91401 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91402 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91403 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91404 2 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 54 C91405 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91406 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91407 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1172 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 463 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91408 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91409 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91410 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91411 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91412 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91413 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91414 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91415 2 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 54 C91416 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1173 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 464 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91417 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91418 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91419 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91420 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91421 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91422 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91423 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91424 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91425 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1174 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 465 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91426 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91427 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91428 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91429 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91430 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91431 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91432 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91433 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91434 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1175 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 466 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91435 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91436 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91437 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91438 2 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 54 C91439 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91440 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91441 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91442 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91443 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 1176 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 467 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91444 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91445 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91446 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91447 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91448 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 C91449 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91450 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1177 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 468 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91451 U 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) AXILLARY REGION-RIGHT WEEK 92 INGUINAL REGION-LEFT WEEK 98 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C 715C, 722C, 729C, 732C 680, 687, 694, 701, 708C, 715C, 722C, 729C, 732C C91452 M 74 APPEARANCE THIN 512 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 512 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 43 295C, 302C, 309C, 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C 1178 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 469 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91452 M 74 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 43 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C C91453 U 105 APPEARANCE THIN 715, 722, 729, 732 DISCHARGE NASAL RED IN COLOR 729, 732 SKIN & PELAGE SORE/SCAB PAWS-HIND 680, 687, 694, 701, 708, 715, 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 1179 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 470 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91454 M 83 EXCRETION NO FECES 566, 568, 575, 578 RESPIRATION AUDIBLE 578 LABORED 578 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA RED HAIR COAT DORSAL-CERVICAL HEAD-CRANIAL 578 566, 568, 575, 578 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505 512, 519, 526, 533, 540, 547, 566, 568, 575, 578 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 554, 561 C91455 U 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 729, 732 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 708, 715, 722 1180 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 471 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91455 U 105 MASS(ES) INGUINAL REGION-LEFT WEEK 92 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C C91456 M 77 APPEARANCE MALOCCLUSION 484, 491, 498, 505, 512, 519, 526, 533 EXCRETION FEW FECES 533 NO FECES 533 EYES RED DISCHARGE EYES 533 SKIN & PELAGE YELLOW HAIR COAT 533 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 1181 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 472 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91457 M 96 BEHAVIOR HYPOACTIVE 667 DISCHARGE NASAL RED IN COLOR 667 RESPIRATION LABORED 667 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 582, 589, 596, 603, 610, 617, 624, 631C, 638C, 645C, 652C, 659C 666-667C C91458 M 63 APPEARANCE PROLAPSED VAGINA 435 DISCHARGE GENITAL RED IN COLOR 428 1182 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 473 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91458 M 63 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421 MASS(ES) VENTRAL-ABDOMINAL WEEK 62 428, 435 C91459 U 105 APPEARANCE MALOCCLUSION 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722, 729, 732 SKIN & PELAGE SORE/SCAB PAWS-HIND 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260 MASS(ES) VENTRAL-ABDOMINAL WEEK 102 708, 715, 722, 729C, 732C 1183 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 474 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91460 D 62 APPEARANCE SWOLLEN VENTRAL-ABDOMINAL THIN 421, 428-429 400, 407, 414, 421, 428-429 DISCHARGE GENITAL RED IN COLOR 372, 379, 386 EXCRETION NONFORMED FECES 428-429 SKIN & PELAGE PALE ENTIRE BODY 421, 428-429 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 393 MASS(ES) VENTRAL-ABDOMINAL WEEK 59 407C, 414C, 421C, 428-429C 1184 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 475 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91461 M 86 APPEARANCE HEAD TILT LEFT MALOCCLUSION THIN 596, 600 43, 50, 57, 64, 71, 78, 85 600 SKIN & PELAGE SORE/SCAB DORSAL YELLOW HAIR COAT 463, 470, 477, 484, 491, 498, 505, 509, 512, 519, 526, 533, 540 547, 554, 561, 568, 575, 582, 589, 596, 600 582, 589, 596, 600 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 92, 99, 106, 113, 120, 127, 134, 141, 148 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232, 239 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330 337, 344 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 VENTRAL-ABDOMINAL-RIGHT WEEK 84 351C, 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C 505C, 509C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C 575C, 582C, 589C, 596C, 600C 582, 589, 596, 600 1185 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 476 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91462 U 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 732 C91463 M 87 BEHAVIOR HYPOACTIVE 608 RECUMBENT LATERAL 608 EYES RED DISCHARGE EYES 596, 608 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 MASS(ES) AXILLARY REGION-LEFT WEEK 71 VENTRAL-ABDOMINAL-RIGHT WEEK 66 491, 498, 505, 512, 519, 526, 533, 540, 547, 554C, 561C, 568C 575C, 582C, 589C, 596C, 603C, 608C 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C 1186 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 477 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91463 M 87 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C 608C C91464 M 74 APPEARANCE THIN 498, 505, 512 EXCRETION FEW FECES 505 NO FECES 498 EYES RED DISCHARGE EYE-LEFT 498, 505 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 498, 505, 512 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 239, 246, 253, 260C, 267C, 274C, 281C, 288C, 295C, 302C, 309C 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C 470C, 477C, 484C, 491C, 498C, 505C, 512C 1187 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 478 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91465 M 90 APPEARANCE DEHYDRATED MALOCCLUSION SWOLLEN NOSE THIN 617, 624 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624 134 582, 589, 596, 603, 610, 617, 624 EXCRETION FEW FECES 547, 554, 561, 568, 575 SKIN & PELAGE SORE/SCAB HEAD-ENTIRE YELLOW HAIR COAT 134, 141 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 C91466 M 93 BEHAVIOR HYPOACTIVE 642, 645-647 DISCHARGE NASAL RED IN COLOR 647 EXCRETION FEW FECES 642, 645 1188 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 479 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91466 M 93 EXCRETION NO FECES 646-647 EYES RED DISCHARGE EYES 642, 645-647 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB PAW-HIND-LEFT YELLOW HAIR COAT 642, 645-647 631, 638, 642, 645-647 647 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) INGUINAL REGION-LEFT WEEK 87 603, 610, 617, 624, 631 1189 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 480 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91467 U 105 SKIN & PELAGE ROUGH HAIR COAT SCALY SKIN TAIL SORE/SCAB LIMBS-HIND PAW-HIND-LEFT PAWS-HIND TAIL 358, 365 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729, 732 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 617, 624, 631, 638, 645, 652, 659, 666 211, 218, 225, 232 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 288, 295, 302, 309, 316, 323, 330, 337, 344 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 351 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 C91468 D 64 APPEARANCE MALOCCLUSION THIN 106, 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 197, 204, 211, 213, 218, 225, 232, 239, 246, 253, 260, 267, 274 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 213, 218, 225, 232, 239, 246, 253, 260 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99 1190 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 481 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91469 D 72 EXCRETION FEW FECES 498, 500 EYES RED DISCHARGE EYE-LEFT 498, 500 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE PERINEAL AREA ROUGH HAIR COAT 372, 379, 386, 393, 400, 407, 414, 421, 428, 498, 500 498, 500 498, 500 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 449, 456, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 463, 470, 477, 484, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 491 92, 99, 106 183, 190, 197 274, 281, 288 365, 435, 442 C91470 M 69 APPEARANCE HUNCHED 477 THIN 477 BEHAVIOR HYPOACTIVE 477 EXCRETION FEW FECES 477 NO FECES 477 1191 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 482 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91470 M 69 EYES PALE EYES 477 RED DISCHARGE EYES 477 RESPIRATION AUDIBLE 477 SKIN & PELAGE BROWN HAIR COAT GENERALIZED 477 COLD TO TOUCH ENTIRE BODY 477 RED HAIR COAT HEAD-ENTIRE 477 VENTRAL-ABDOMINAL 477 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 1192 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 483 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91471 M 97 APPEARANCE THIN 673, 675 EXCRETION NO FECES 675 EYES RED DISCHARGE EYES 673, 675 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-RIGHT 526, 533 638, 645, 652, 659 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 337, 463, 470, 582, 589, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 344, 393, 400, 407, 477, 484, 491, 498, 596, 603, 610, 617, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 414, 421, 428, 435, 505, 540, 547, 554, 624, 631, 666 92, 99, 106 183, 190, 197 274, 281, 288 442, 449, 456 561, 568, 575 MASS(ES) VENTRAL-ABDOMINAL WEEK 44 WEEK 74 VENTRAL-ABDOMINAL-RIGHT WEEK 51 VENTRAL-CERVICAL-LEFT WEEK 48 302, 309, 316, 323, 330 512C, 519C 351, 358, 365, 372, 379, 386 330 1193 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 484 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91472 M 98 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C 617C, 624C, 631C, 638C, 641C, 645C, 652C, 659C, 666C, 673C, 680C C91473 U 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) AXILLARY REGION-LEFT WEEK 87 VENTRAL-CERVICAL-LEFT WEEK 87 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687 694, 701, 708C, 715C, 722C, 729C, 732C 603, 610, 617, 624, 631, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C, 694C, 701C 1194 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 485 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91474 M 93 APPEARANCE HEAD TILT LEFT MALOCCLUSION THIN 638, 645 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645 617, 624, 631, 638, 645 BEHAVIOR HYPOACTIVE 638, 645 EXCRETION NO FECES 183 SKIN & PELAGE ALOPECIA ROUGH HAIR COAT 617, 624, 631 358, 365, 372 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113 1195 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 486 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91475 M 100 APPEARANCE SWOLLEN VENTRAL-ABDOMINAL 582, 589, 596 DISCHARGE GENITAL RED IN COLOR 288, 358, 365, 372, 379, 386, 547, 554, 582 EXCRETION NO FECES NONFORMED FECES 694, 697 603, 610 EYES CLEAR DISCHARGE EYE-RIGHT RED DISCHARGE EYE-RIGHT 316, 323, 491, 498, 505, 512, 519, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 697 295, 302, 309 SKIN & PELAGE ALOPECIA BROWN HAIR COAT VENTRAL-ABDOMINAL PALE ENTIRE BODY RED HAIR COAT PERINEAL AREA ROUGH HAIR COAT SORE/SCAB LIMBS-HIND PAW-HIND-RIGHT 617, 624, 631, 638, 645, 652 694 358, 365, 372, 379, 386 697 358, 365, 372 673, 680, 687, 694, 697 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 1196 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 487 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91475 M 100 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 330 337, 344, 351, 393, 400, 407, 414 C91476 M 80 APPEARANCE THIN 547 BEHAVIOR HYPOACTIVE 557 DISCHARGE NASAL RED IN COLOR 547 EXCRETION FEW FECES NO FECES 533, 540 557 EYES RED DISCHARGE EYES 533, 540, 547, 554, 557 RESPIRATION AUDIBLE 557 IRREGULAR 557 1197 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 488 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91476 M 80 SKIN & PELAGE YELLOW HAIR COAT 554, 557 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C91477 M 86 APPEARANCE THIN 547, 554, 561, 568, 575, 582, 589, 596 BEHAVIOR HYPOACTIVE 589, 596 EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL 582, 589, 596 582, 589, 596 SKIN & PELAGE YELLOW HAIR COAT 582, 589, 596 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309 1198 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 489 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91477 M 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C C91478 D 85 SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL SORE/SCAB DORSAL-THORACIC-LEFT 582, 589, 593 547, 554, 561, 568, 575, 582, 589, 593 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) AXILLARY REGION-LEFT WEEK 78 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 593C 1199 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 490 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91479 U 105 EYES RED DISCHARGE EYE-LEFT 428, 435, 442, 449 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 505, 512, 596, 603, 687, 694, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 456, 463, 470, 477, 547, 554, 561, 568, 638, 645, 652, 659, 729, 732 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 484, 491, 498 575, 582, 589 666, 673, 680 C91480 U 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 732 C91481 M 73 APPEARANCE HUNCHED 509 THIN 509 BEHAVIOR ATAXIC 509 1200 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 491 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91481 M 73 EXCRETION FEW FECES 491 SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL RED HAIR COAT HEAD-ENTIRE SORE/SCAB VENTRAL-ABDOMINAL YELLOW HAIR COAT 509 470 509 470, 477 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C 498C, 505C, 509C C91482 M 93 APPEARANCE DEHYDRATED HEAD TILT RIGHT HUNCHED THIN 617, 624, 631, 638 638, 642, 645 642, 645 582, 589, 596, 603, 609-610, 617, 624, 631, 638, 642, 645 1201 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 492 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91482 M 93 BEHAVIOR ATAXIC 642, 645 EXCRETION FEW FECES 617, 624 EYES PALE EYES RED DISCHARGE EYES 642, 645 575, 582, 589, 596, 603, 609-610, 617, 624, 631, 638, 642, 645 SKIN & PELAGE ALOPECIA ROUGH HAIR COAT 617, 624, 631 617, 624, 631, 638, 642, 645 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 400, 407, 491 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 414, 421, 428, 435, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 442, 449, 456, 463, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 470, 477, 484 MASS(ES) MOUTH WEEK 56 VENTRAL-ABDOMINAL-RIGHT WEEK 72 386, 393 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C 575C, 582C, 589C, 596C, 603C, 609-610C, 617C, 624C, 631C, 638C 642C, 645C 1202 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 493 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91483 D 96 APPEARANCE MALOCCLUSION SWOLLEN LIMB-HIND-RIGHT 393, 400, 407 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 EYES RED DISCHARGE EYE-RIGHT 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617 624, 631, 638, 645, 652, 659, 666 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 C91484 M 71 APPEARANCE HUNCHED MALOCCLUSION THIN 488, 491 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 488, 491 477, 484, 488, 491 BEHAVIOR HYPOACTIVE 488, 491 SKIN & PELAGE COLD TO TOUCH ENTIRE BODY 491 1203 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 494 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91484 M 71 SKIN & PELAGE PALE ENTIRE BODY 488, 491 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 C91485 U 105 APPEARANCE MALOCCLUSION 99, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729 732 DISCHARGE GENITAL RED IN COLOR 281, 288 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92 1204 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 495 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91486 M 92 APPEARANCE BENT TAIL DEHYDRATED MISSING TAIL-PROXIMAL SWOLLEN MOUTH THIN 134 617, 624, 631, 638 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498 505, 512, 519, 526, 533, 540, 547, 554, 561, 564, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 640 617, 624, 631, 638, 640 603, 610, 617, 624, 631, 638, 640 BEHAVIOR ATAXIC HYPOACTIVE 610, 617, 624, 631, 638, 640 640 EXCRETION FEW FECES 564, 568, 575, 617, 624, 631, 638, 640 EYES OPAQUE EYE-RIGHT 29, 36, 43, 50, 57, 134, 141, 148, 155, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 582, 589, 596, 603, 64, 71, 78, 85, 92, 162, 169, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 610, 617, 624, 631, 99, 106, 113, 120, 127 190, 197, 204, 211, 218 281, 288, 295, 302, 309 372, 379, 386, 393, 400 463, 470, 477, 484, 491 554, 561, 564, 568, 575 638, 640 1205 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 496 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91486 M 92 SKIN & PELAGE PALE ENTIRE BODY 564, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 640 YELLOW HAIR COAT 610, 617, 624, 631, 638, 640 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C91487 U 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 732 C91488 U 105 APPEARANCE MALOCCLUSION 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 SKIN & PELAGE SORE/SCAB DORSAL-THORACIC-LEFT 554, 561, 568, 575 NORMAL NO REMARKABLE OBSERVATIONS 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 1206 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 497 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91488 U 105 NORMAL NO REMARKABLE OBSERVATIONS 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 C91489 D 81 DISCHARGE NASAL RED IN COLOR 120 SKIN & PELAGE ALOPECIA 120 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 127, 211, 218, 302, 309, 393, 400, 484, 491, 22, 29, 36, 43, 50, 134, 141, 148, 155, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 57, 64, 71, 78, 85, 162, 169, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540 92, 99, 106 190, 197, 204 281, 288, 295 372, 379, 386 463, 470, 477 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 79 547C, 554C, 561C C91490 M 83 APPEARANCE MALOCCLUSION SWOLLEN MOUTH 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 519, 526, 533, 540, 547, 554, 561, 565, 568, 575 428 EYES RED DISCHARGE EYE-LEFT 358, 365, 372 1207 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 498 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 100 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C91490 M 83 SKIN & PELAGE BROWN HAIR COAT GENERALIZED 456, 463 BLACK SKIN VENTRAL-THORACIC-RIGHT 540, 547 ROUGH HAIR COAT 358, 365, 372, 379, 386, 393, 400, 407 SORE/SCAB MOUTH 435, 442, 449, 540, 547 VENTRAL-ABDOMINAL 547, 554, 561, 565, 568, 575 VENTRAL-THORACIC-RIGHT 565, 568, 575 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 414, 421 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 VENTRAL-THORACIC-RIGHT WEEK 75 470C, 477C, 484C, 491C 519C, 526C, 533C, 540C, 547C, 554C, 561C, 565C, 568C, 575C 1208 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 499 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91451 F6 638 92 TO 666 96 MASS(ES) AXILLARY REGION-RIGHT WEEK 92 10 X 10MM 673 97 TO 701 101 MASS(ES) AXILLARY REGION-RIGHT WEEK 92 20 X 20MM 708 102 TO 722 104 MASS(ES) AXILLARY REGION-RIGHT WEEK 92 30 X 25MM 729 105 TO 732 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 92 30 X 30MM 708 102 MASS(ES) INGUINAL REGION-LEFT WEEK 98 10 X 10MM 715 103 TO 722 104 MASS(ES) INGUINAL REGION-LEFT WEEK 98 15 X 15MM 729 105 TO 732 105 MASS(ES) INGUINAL REGION-LEFT WEEK 98 15 X 20MM C91452 F6 295 43 TO 302 44 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 43 15 X 15MM 1209 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 500 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91452 F6 309 45 TO 323 47 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 43 20 X 20MM 330 48 TO 379 55 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 43 25 X25MM 386 56 TO 407 59 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 43 35 X 25MM 414 60 TO 477 69 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 43 40 X 30MM 484 70 TO 491 71 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 43 40 X 40MM 498 72 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 43 70 X 60MM 505 73 TO 512 74 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 43 80 X 70MM C91455 F6 638 92 TO 701 101 MASS(ES) INGUINAL REGION-LEFT WEEK 92 10 X 10MM 1210 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 501 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91457 F6 631 91 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 10 X 10MM 638 92 TO 667 96 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 15 X 15MM C91459 F6 729 105 TO 732 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 102 20 X 15MM C91460 F6 407 59 TO 429 62 MASS(ES) VENTRAL-ABDOMINAL WEEK 59 40 X 40MM C91461 F6 351 51 TO 600 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 10 X 10MM C91463 F6 554 80 TO 568 82 MASS(ES) AXILLARY REGION-LEFT WEEK 71 10 X 10MM 575 83 TO 608 87 MASS(ES) AXILLARY REGION-LEFT WEEK 71 20 X 20MM 456 66 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 10 X 15MM 1211 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 502 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91463 F6 463 67 TO 477 69 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 20 X 30MM 484 70 TO 491 71 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 25 X 40MM 498 72 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 25 X 25MM 589 85 TO 608 87 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 25 X 30MM C91464 F6 260 38 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 15 X 10MM 267 39 TO 281 41 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 15 X 10MM 288 42 TO 407 59 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 25 X 20MM 414 60 TO 491 71 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 30 X 25MM 1212 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 503 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91464 F6 498 72 TO 512 74 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 40 X 40MM C91471 F6 512 74 TO 519 75 MASS(ES) VENTRAL-ABDOMINAL WEEK 74 10 X 10MM C91472 F6 463 67 TO 477 69 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 30 X 30MM 484 70 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 30 X 40MM 491 71 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 40 X 50MM 498 72 TO 512 74 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 50 X 60MM 519 75 TO 533 77 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 55 X 75MM 540 78 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 75 X 75MM 1213 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 504 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91472 F6 547 79 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 80 X 75MM 561 81 TO 568 82 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 80 X 80MM 575 83 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 80 X 90MM 589 85 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 90 X 100MM 638 92 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 100 X 115MM C91473 F6 708 102 TO 732 105 MASS(ES) AXILLARY REGION-LEFT WEEK 87 20 X 20MM 638 92 TO 701 101 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 87 10 X 10MM C91477 F6 316 46 TO 323 47 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 10 X 10MM 1214 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 505 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91477 F6 330 48 TO 344 50 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 20 X 20MM 351 51 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 25 X 25MM 358 52 TO 365 53 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 25 X 35MM 372 54 TO 407 59 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 35 X 40MM 414 60 TO 421 61 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 45 X 45MM 428 62 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 55 X 50MM 435 63 TO 512 74 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 55 X 60MM 519 75 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 70 X 75MM 1215 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 506 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91477 F6 526 76 TO 533 77 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 60 X 55MM 540 78 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 60 X 65MM 589 85 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 60 X 70MM C91478 F6 540 78 MASS(ES) AXILLARY REGION-LEFT WEEK 78 20 X 30MM 547 79 TO 568 82 MASS(ES) AXILLARY REGION-LEFT WEEK 78 30 X 40MM 575 83 TO 582 84 MASS(ES) AXILLARY REGION-LEFT WEEK 78 70 X 60MM 589 85 TO 593 85 MASS(ES) AXILLARY REGION-LEFT WEEK 78 70 X 70MM C91481 F6 421 61 TO 428 62 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 15 X 15MM 1216 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 507 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91481 F6 435 63 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 30 X 20MM 442 64 TO 449 65 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 35 X 20MM 456 66 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 40 X 25MM 505 73 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 40 X 35MM 509 73 TO 509 73 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 50 X 40MM C91482 F6 498 72 TO 526 76 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 15 X 15MM 533 77 TO 547 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 20 X 20MM 554 80 TO 589 85 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 30 X 20MM 1217 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 508 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C91482 F6 596 86 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 30 X 30MM 638 92 TO 645 93 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 30 X 35MM C91489 F6 547 79 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 79 20 X 10MM 554 80 TO 561 81 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 79 20 X 15MM C91490 F6 470 68 TO 491 71 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 20 X 20MM 519 75 TO 526 76 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 75 20 X 20MM 533 77 TO 540 78 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 75 50 X 50MM 547 79 TO 575 83 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 75 20 X 30MM 1218 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 509 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F7 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91491 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91492 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91493 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91494 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91495 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91496 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91497 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91498 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91499 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1219 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 510 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F7 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91500 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91501 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91502 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91503 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91504 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91505 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91506 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91507 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91508 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1220 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 511 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F7 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91509 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91510 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91511 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91512 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91513 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91514 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91515 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91516 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91517 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1221 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 512 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F7 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91518 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91519 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91520 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91521 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91522 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91523 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91524 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91525 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91526 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1222 Appendix 2 Covance 6329-212 3M T-6316.1 Individual Clinical Observations PAGE: 513 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F7 DOSE: 300 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C91527 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91528 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91529 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C91530 6 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1223 Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Author: Peter J. Thomford, PhD Study Completion Date: December 13, 2001 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-212 Sponsor Study Identification: 3M T-6316.1 Volume IV of XII Page 1224 of 4873 Covance 6329-212 3M T-6316.1 VOLUME IV OF XII CONTENTS Page APPENDIX 3............................................................................................................ 1226 Individual Body Weight Data (g)........................................................................... 1227 APPENDIX 4............................................................................................................ 1306 Individual Food Consumption Data (g).................................................................. 1307 Individual Test Material Consumption Data (mg/kg/day)....................................... 1384 APPENDIX 5............................................................................................................ 1456 Individual Clinical Hematology Data..................................................................... 1457 Individual Clinical Chemistry Data........................................................................ 1499 Individual Clinical Urinalysis Data........................................................................ 1541 Individual Urine Chemistry Data ........................................................................... 1583 1225 APPENDIX 3 Individual Body Weight Data (g) Covance 6329-212 3M T-6316.1 1226 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C90711 267 313 357 386 402 431 446 467 480 494 C90712 253 304 345 376 403 425 445 462 475 492 C90713 280 329 372 413 444 475 495 515 531 554 C90714 251 292 326 363 389 415 440 456 478 498 C90715 310 350 393 423 438 459 478 492 508 528 C90716 235 282 326 358 388 411 435 450 470 486 C90717 278 319 362 403 428 448 481 496 C90718 252 292 339 380 410 433 462 478 C90719 223 265 303 337 C90720 280 332 380 427 462 496 519 539 551 575 C90721 288 338 387 437 487 533 569 594 628 637 C90722 246 288 325 354 378 403 414 433 452 468 C90723 271 328 383 418 453 482 511 530 540 567 C90724 257 300 339 372 398 424 448 459 477 489 C90725 258 303 345 379 C90726 243 288 336 373 404 435 455 459 478 495 C90727 293 345 398 445 480 514 540 563 581 605 C90728 250 294 332 373 397 430 454 474 495 511 C90729 277 323 369 408 441 470 494 502 528 541 C90730 277 318 355 392 415 439 467 483 506 526 C90731 258 311 367 410 443 477 503 524 548 572 C90732 295 349 391 442 C90733 226 278 324 363 397 429 451 473 487 511 C90734 307 366 412 459 494 524 556 576 601 618 C90735 264 306 347 373 400 423 437 449 466 477 C90736 263 302 340 370 399 429 453 474 497 513 C90737 254 298 341 378 412 444 470 486 C90738 247 293 329 355 384 405 427 444 456 474 C90739 263 308 350 380 408 432 456 466 489 499 C90740 250 289 335 368 396 423 442 466 490 510 C90741 248 292 327 358 386 412 433 449 466 479 1227 Covance 6329-212 3M T-6316.1 Appendix 3 Individual Body Weight Data (g) PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C90742 245 295 332 369 402 433 461 473 488 509 C90743 267 315 359 400 432 465 493 506 536 559 C90744 274 317 354 390 415 441 461 475 490 505 C90745 246 280 312 344 373 396 415 427 439 457 C90746 269 319 367 414 444 476 504 530 560 573 C90747 261 304 344 375 399 424 437 455 472 487 C90748 284 335 374 408 431 452 477 489 503 514 C90749 230 270 312 342 368 390 404 420 434 452 C90750 246 294 335 374 402 426 441 456 472 491 C90751 259 306 348 380 401 424 445 458 474 492 C90752 301 359 414 451 484 510 536 551 578 600 C90753 257 306 346 379 C90754 278 333 373 414 447 467 496 507 526 539 C90755 279 333 375 414 446 478 512 540 569 595 C90756 214 258 293 327 354 378 401 416 C90757 283 336 380 425 456 486 512 534 557 567 C90758 251 296 340 380 415 446 467 488 504 520 C90759 235 281 327 372 403 429 453 468 489 499 C90760 259 309 355 389 411 440 465 474 497 511 C90761 260 305 340 370 403 428 451 466 485 502 C90762 229 277 318 346 374 402 424 440 456 472 C90763 289 344 384 427 464 492 515 529 551 569 C90764 255 300 341 380 407 429 446 459 470 490 C90765 273 332 382 423 462 490 516 540 566 591 C90766 262 310 350 388 405 432 463 481 499 504 C90767 222 259 294 333 359 393 416 428 450 467 C90768 247 287 327 360 381 405 416 421 445 459 C90769 267 316 357 397 426 452 472 484 C90770 238 282 320 349 373 397 417 426 439 439 C90771 266 311 351 382 413 441 461 470 486 504 C90772 253 304 352 386 416 442 465 479 497 514 1228 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C90773 259 311 361 394 425 453 476 496 514 530 C90774 257 301 343 380 404 430 450 462 481 496 C90775 262 305 344 381 C90776 287 338 379 420 454 477 509 526 552 569 C90777 280 326 370 412 452 483 515 537 569 588 C90778 287 326 361 397 420 444 460 466 486 496 C90779 242 295 341 375 410 431 452 461 476 497 C90780 252 300 344 392 431 461 487 503 539 560 GROUP: MALE 2 - 3 PPM C90781 281 320 359 394 423 436 456 472 500 518 C90782 255 303 343 374 407 429 449 457 477 495 C90783 223 263 301 333 363 391 413 418 440 459 C90784 253 298 340 373 400 422 441 447 470 492 C90785 254 302 345 379 404 432 456 469 495 514 C90786 265 311 355 386 413 437 456 464 488 495 C90787 249 300 339 377 411 442 467 486 515 534 C90788 260 311 356 396 422 451 470 487 510 526 C90789 279 333 385 440 C90790 279 328 374 417 439 476 498 513 548 566 C90791 221 263 303 335 360 388 408 419 443 454 C90792 249 295 339 380 409 443 463 482 508 530 C90793 246 295 337 373 402 429 449 464 484 500 C90794 245 297 347 385 421 452 475 493 525 544 C90795 271 318 361 392 418 441 451 469 493 512 C90796 284 332 367 398 418 433 457 472 497 511 C90797 249 295 333 373 405 435 457 476 504 520 C90798 270 320 370 410 402 434 457 474 492 521 C90799 242 288 336 368 396 419 433 449 473 484 C90800 286 332 377 414 439 474 499 518 543 562 1229 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 3 PPM C90801 271 312 352 388 415 441 460 477 503 518 C90802 230 268 302 334 360 384 403 414 431 448 C90803 261 317 373 413 449 487 510 515 546 550 C90804 306 354 409 455 489 523 550 571 597 619 C90805 279 326 368 402 427 461 488 498 523 539 C90806 246 284 319 347 368 394 403 414 421 440 C90807 288 330 374 415 438 464 477 482 514 530 C90808 276 326 367 406 440 471 501 508 539 556 C90809 244 288 324 351 391 411 436 451 473 483 C90810 269 316 368 406 438 472 495 511 539 557 C90811 261 306 354 393 419 447 468 483 517 529 C90812 256 311 356 394 426 450 478 490 515 535 C90813 239 278 314 345 366 389 404 375 C90814 235 274 312 348 C90815 253 297 344 387 419 451 482 495 524 532 C90816 293 342 393 424 451 473 495 517 535 556 C90817 256 302 341 375 C90818 257 302 346 387 412 442 464 478 499 515 C90819 281 331 377 418 446 477 500 510 537 551 C90820 267 313 348 383 414 445 469 482 508 523 C90821 287 339 388 438 470 506 538 553 579 597 C90822 222 259 292 327 349 377 394 408 428 445 C90823 228 270 309 344 354 384 399 409 436 451 C90824 234 288 333 378 403 436 463 469 496 511 C90825 271 318 357 396 419 451 482 500 525 543 C90826 262 310 359 393 418 455 473 498 530 540 C90827 243 291 334 379 410 439 465 485 508 528 C90828 251 303 352 400 435 464 497 508 534 556 C90829 272 325 378 418 451 483 508 526 554 581 C90830 263 312 351 393 423 457 475 489 512 530 C90831 265 314 351 385 415 444 468 485 519 540 1230 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 3 PPM C90832 255 311 361 404 443 467 494 507 532 546 C90833 271 315 358 399 C90834 273 324 378 426 C90835 276 318 355 388 406 434 451 464 484 496 C90836 247 294 330 363 396 417 436 450 475 488 C90837 235 274 308 344 371 392 414 425 448 463 C90838 300 359 416 461 490 523 549 564 587 606 C90839 294 347 395 440 481 513 547 557 593 605 C90840 245 293 341 385 418 455 476 495 523 548 GROUP: MALE 3 - 30 PPM C90841 230 277 317 345 372 404 426 444 471 482 C90842 220 254 278 305 328 348 364 380 395 408 C90843 245 294 338 376 409 442 471 480 459 524 C90844 271 314 350 393 401 427 440 446 468 482 C90845 286 330 358 398 415 446 465 474 500 513 C90846 251 294 326 358 C90847 251 290 333 371 397 419 439 448 467 486 C90848 246 288 329 369 402 432 451 467 495 514 C90849 264 312 359 398 425 456 486 494 522 541 C90850 254 289 327 358 383 405 423 439 456 468 C90851 249 293 338 381 406 433 451 457 476 486 C90852 264 307 347 381 410 431 457 463 488 498 C90853 238 273 304 337 C90854 267 310 352 384 412 437 459 480 501 516 C90855 267 315 360 400 422 465 486 495 528 540 C90856 261 314 356 392 421 448 469 486 503 516 C90857 273 317 360 394 421 447 471 484 498 519 C90858 228 263 298 324 350 371 389 405 424 430 C90859 269 310 360 403 432 459 480 494 507 518 1231 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90860 234 275 312 340 361 381 394 396 414 420 C90861 269 302 339 371 400 432 453 461 483 493 C90862 206 234 267 291 308 336 350 359 374 381 C90863 286 350 409 465 500 537 562 582 607 632 C90864 263 305 344 375 394 415 433 444 467 472 C90865 252 294 325 362 382 402 421 430 451 458 C90866 295 332 364 390 C90867 255 296 335 363 388 407 425 445 457 464 C90868 292 333 377 427 456 485 511 520 546 563 C90869 263 315 366 410 438 467 486 498 515 535 C90870 274 317 359 387 422 449 475 489 516 531 C90871 256 303 345 377 405 430 446 455 472 482 C90872 276 315 357 390 413 438 459 466 487 498 C90873 299 342 393 424 460 488 515 527 546 558 C90874 280 333 383 426 456 490 520 540 571 585 C90875 238 285 328 362 387 415 430 446 463 487 C90876 294 350 407 460 488 512 528 545 568 594 C90877 255 294 333 372 396 426 435 444 457 450 C90878 223 260 299 330 351 374 391 405 432 440 C90879 257 308 348 385 408 440 456 471 491 508 C90880 281 335 388 429 454 485 505 521 549 566 C90881 264 308 361 401 433 460 479 496 515 535 C90882 265 313 358 389 413 442 464 479 498 511 C90883 263 315 357 397 431 451 471 484 505 515 C90884 248 289 329 370 391 418 438 447 463 473 C90885 255 298 343 375 403 424 439 452 465 474 C90886 271 321 362 400 428 464 484 495 520 543 C90887 274 314 358 391 418 442 460 472 493 506 C90888 282 320 357 397 415 432 444 457 476 486 C90889 249 299 343 378 396 417 435 454 469 481 C90890 283 321 358 398 1232 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90891 268 315 375 414 C90892 214 234 252 277 299 323 343 359 372 385 C90893 243 286 324 355 379 409 431 440 458 471 C90894 267 310 342 363 390 419 432 451 460 475 C90895 245 283 320 344 356 381 404 420 443 456 C90896 276 314 356 387 409 428 445 458 458 477 C90897 286 327 376 418 454 489 508 529 547 567 C90898 265 302 337 374 398 426 445 459 477 484 C90899 231 264 303 334 352 376 389 404 423 429 C90900 256 290 334 374 403 431 444 459 474 487 GROUP: MALE 4 - 100 PPM C90901 243 278 312 340 365 389 402 414 423 431 C90902 243 284 319 346 374 396 410 421 435 444 C90903 237 273 306 332 359 379 398 409 422 431 C90904 263 304 343 368 391 410 422 439 451 457 C90905 241 276 307 337 361 378 393 406 419 428 C90906 261 292 325 358 388 411 426 443 461 484 C90907 266 299 328 361 382 410 426 437 449 456 C90908 274 315 351 383 415 436 460 472 492 495 C90909 275 325 374 411 445 468 489 513 539 546 C90910 239 276 308 341 367 392 420 434 449 458 C90911 259 297 334 361 391 415 438 453 468 487 C90912 282 323 361 396 429 459 479 497 509 527 C90913 239 283 329 366 396 419 430 393 420 456 C90914 275 326 366 404 432 462 483 504 521 533 C90915 269 308 348 388 425 451 481 494 512 522 C90916 231 268 304 331 356 374 389 404 422 430 C90917 258 306 346 385 422 450 467 483 500 503 C90918 299 341 387 415 440 468 488 508 525 528 1233 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90919 261 306 348 381 416 444 468 486 501 515 C90920 256 293 329 360 390 412 427 443 464 479 C90921 263 301 337 366 395 418 443 463 481 496 C90922 265 294 334 364 388 410 423 432 453 462 C90923 298 323 356 385 409 426 445 456 470 480 C90924 250 283 321 359 383 414 437 457 478 490 C90925 275 307 334 358 374 398 414 419 430 437 C90926 274 314 357 393 418 451 472 487 510 529 C90927 251 296 338 375 405 433 450 458 479 494 C90928 252 289 325 355 382 407 423 437 449 462 C90929 228 266 300 324 345 370 386 393 407 422 C90930 270 313 349 375 400 409 429 448 465 472 C90931 274 307 340 367 398 430 452 462 477 497 C90932 246 273 297 323 341 354 373 381 403 416 C90933 270 320 368 397 430 457 477 490 512 524 C90934 241 280 316 342 364 391 405 417 429 442 C90935 284 324 370 398 C90936 235 278 319 349 368 390 410 427 441 453 C90937 254 291 333 367 391 399 424 439 444 453 C90938 222 257 296 323 349 365 381 401 421 434 C90939 247 282 316 342 C90940 287 340 379 415 454 477 497 508 530 547 C90941 264 306 347 382 412 448 468 489 510 516 C90942 249 285 325 355 C90943 260 301 341 370 393 413 427 440 441 461 C90944 224 255 288 316 337 356 373 388 410 423 C90945 254 287 324 348 370 392 403 422 439 446 C90946 267 305 340 370 400 432 453 459 471 483 C90947 273 304 335 363 389 406 428 437 442 453 C90948 263 303 340 368 389 411 426 434 452 465 C90949 252 283 307 330 347 352 366 360 355 344 1234 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90950 263 313 356 387 410 436 456 471 494 503 C90951 279 315 357 382 390 421 441 456 474 479 C90952 278 335 384 433 465 492 519 532 549 560 C90953 247 293 332 368 398 421 437 457 472 481 C90954 223 267 304 335 357 382 398 413 428 438 C90955 274 319 357 386 403 431 444 459 466 476 C90956 258 302 346 383 405 441 463 485 500 506 C90957 238 276 319 342 367 395 416 384 423 441 C90958 269 314 354 385 407 436 455 465 475 485 C90959 247 291 330 368 388 415 437 454 479 486 C90960 255 297 335 364 390 422 439 457 471 485 C90961 228 269 301 334 C90962 273 323 365 397 423 452 475 493 509 512 C90963 279 323 367 407 435 467 481 502 516 532 C90964 298 348 402 441 474 502 524 539 561 572 C90965 228 266 304 314 348 367 382 387 402 410 C90966 295 339 387 418 C90967 243 281 320 352 380 411 433 447 465 475 C90968 232 271 309 340 366 393 414 433 448 461 C90969 274 318 366 399 425 450 473 493 511 531 C90970 240 282 316 344 367 386 403 409 424 433 GROUP: MALE 5 - 300 PPM C90971 218 233 242 256 268 277 290 291 C90972 260 274 295 312 319 325 327 326 C90973 268 309 344 365 379 399 412 418 C90974 250 286 317 339 356 368 384 395 C90975 267 299 331 363 376 393 406 420 C90976 235 269 293 313 327 340 352 354 C90977 259 287 314 327 340 347 349 354 1235 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 300 PPM C90978 263 293 325 344 364 373 381 387 C90979 244 271 301 322 333 351 359 372 C90980 253 296 330 357 371 396 403 407 C90981 257 292 326 349 360 377 391 398 C90982 252 287 326 355 377 391 414 419 C90983 209 227 248 257 263 273 285 281 C90984 281 309 339 361 C90985 247 276 300 313 322 339 340 346 C90986 276 305 339 362 381 395 402 402 C90987 266 297 324 341 363 376 386 399 C90988 257 285 309 319 328 332 340 335 C90989 268 312 349 373 399 420 430 442 C90990 271 297 325 346 362 378 397 409 C90991 237 272 315 337 362 377 390 401 C90992 258 292 325 349 365 383 399 409 C90993 277 304 336 362 384 408 415 407 C90994 284 317 346 371 386 410 424 424 C90995 268 298 326 348 367 382 392 403 C90996 284 322 354 377 392 405 412 420 C90997 269 296 324 334 345 359 368 374 C90998 244 271 299 322 337 360 380 392 C90999 273 303 328 345 363 378 388 392 C91000 242 277 297 310 323 333 333 332 C91001 275 317 350 377 407 436 453 465 C91002 241 269 294 314 326 333 339 344 C91003 246 282 311 325 341 354 365 C91004 258 279 307 326 339 348 355 357 C91005 244 274 306 322 C91006 245 265 288 304 310 318 317 311 C91007 252 286 315 336 357 369 383 395 C91008 224 253 283 299 309 320 328 331 1236 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 300 PPM C91009 285 312 337 349 355 364 374 378 C91010 257 278 304 321 EXCLUDE 342 342 350 C91011 254 294 322 349 370 397 412 430 C91012 239 266 292 314 327 342 344 346 C91013 304 343 377 395 407 423 435 449 C91014 224 253 279 299 317 335 344 355 C91015 270 303 330 350 361 378 390 406 C91016 252 278 306 321 334 348 356 366 C91017 273 299 324 335 347 362 363 364 C91018 285 327 365 393 411 430 443 463 C91019 258 287 322 345 359 368 383 386 C91020 238 267 290 313 324 335 343 348 C91021 251 287 323 350 376 393 401 307 C91022 273 307 345 367 386 408 418 433 C91023 253 285 318 345 368 389 404 416 C91024 210 237 270 285 C91025 356 390 419 438 454 465 478 C91026 268 292 319 336 359 377 386 397 C91027 227 256 290 310 330 339 350 359 C91028 262 299 337 356 380 406 419 430 C91029 245 280 306 322 334 346 352 C91030 247 280 311 331 355 374 393 404 C91031 238 262 291 301 318 326 328 323 C91032 240 281 324 356 378 407 418 419 C91033 260 291 321 341 C91034 233 267 302 329 351 371 380 390 C91035 273 304 329 348 354 380 397 408 C91036 261 289 306 326 338 351 357 358 C91037 282 315 348 366 C91038 276 301 334 348 374 385 395 405 C91039 277 320 356 384 407 429 440 437 1237 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 300 PPM C91040 271 297 323 345 361 380 388 395 GROUP: MALE 6 - 100 PPM C91041 266 312 354 386 425 452 476 502 520 542 C91042 248 292 326 351 371 395 401 416 428 440 C91043 257 291 322 344 370 396 412 430 449 462 C91044 259 298 331 358 395 414 430 447 459 468 C91045 241 298 343 384 423 448 476 497 513 528 C91046 249 297 340 374 406 440 377 C91047 240 274 306 334 361 383 394 416 380 413 C91048 259 300 339 369 388 414 429 443 460 471 C91049 256 305 358 393 429 456 477 503 520 536 C91050 264 307 349 383 419 435 461 470 504 497 C91051 261 303 343 377 403 439 451 477 500 512 C91052 270 322 370 407 447 478 501 522 546 557 C91053 269 307 341 365 385 407 415 431 453 457 C91054 247 297 348 382 409 442 455 477 505 507 C91055 264 310 355 390 422 454 477 499 521 537 C91056 254 292 333 359 387 412 422 441 400 437 C91057 280 320 360 389 414 440 453 475 500 509 C91058 267 310 353 379 407 433 443 459 470 476 C91059 254 294 336 370 397 429 445 461 481 493 C91060 238 268 298 324 346 372 379 398 412 420 C91061 271 309 345 380 408 436 448 473 494 505 C91062 242 289 329 365 389 415 433 459 478 487 C91063 215 249 282 308 326 348 362 373 387 389 C91064 248 284 314 345 377 414 426 447 470 478 C91065 237 278 313 342 371 396 410 427 443 450 C91066 270 314 349 386 413 440 453 473 492 500 C91067 257 303 339 369 397 420 437 461 481 491 1238 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 100 PPM C91068 217 244 262 272 279 281 273 276 276 221 C91069 239 285 320 349 374 396 417 434 465 476 C91070 261 301 331 359 386 406 416 433 450 463 C91071 279 329 370 410 444 474 494 520 540 551 C91072 224 264 301 334 355 378 389 410 421 427 C91073 290 333 380 415 444 470 485 502 521 537 C91074 234 277 312 340 365 383 393 415 427 437 C91075 246 288 324 354 378 405 414 435 454 459 C91076 276 312 351 373 392 413 367 409 439 453 C91077 231 274 311 346 372 399 411 431 451 463 C91078 245 287 322 353 379 400 411 431 445 448 C91079 287 328 360 389 414 439 455 476 488 495 C91080 266 307 339 364 388 407 416 436 453 465 GROUP: MALE 7 - 300 PPM C91081 252 282 301 329 350 363 382 391 C91082 240 267 293 312 321 337 338 350 C91083 241 269 286 304 320 331 340 352 C91084 250 272 301 325 342 351 362 374 C91085 236 272 296 312 321 334 331 331 C91086 262 292 316 340 353 372 381 397 C91087 252 283 314 335 346 356 353 362 C91088 251 280 305 328 345 352 355 361 C91089 266 301 333 360 386 406 421 440 C91090 269 294 329 354 372 388 399 418 C91091 251 283 313 329 353 369 372 383 C91092 266 305 335 361 386 400 413 425 C91093 262 291 321 340 336 371 384 402 C91094 243 274 298 315 329 332 343 355 C91095 275 303 330 347 362 369 385 385 1239 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 7 - 300 PPM C91096 243 268 288 311 328 341 348 353 C91097 261 302 328 346 362 383 385 401 C91098 283 316 337 355 376 392 394 406 C91099 240 267 274 280 290 292 C91100 259 285 307 317 340 350 349 361 C91101 285 319 349 374 394 411 424 432 C91102 252 282 306 323 340 351 361 369 C91103 233 263 287 318 324 332 347 352 C91104 202 221 249 266 283 295 300 314 C91105 272 291 316 326 343 352 356 367 C91106 263 292 318 344 355 367 383 396 C91107 222 250 280 304 323 338 344 355 C91108 247 276 302 327 347 366 377 395 C91109 238 268 296 310 330 343 351 364 C91110 257 286 311 338 356 375 381 391 C91111 237 269 298 312 331 352 365 378 C91112 272 304 344 371 394 408 420 439 C91113 242 269 290 306 313 322 311 327 C91114 295 331 360 393 411 429 437 447 C91115 280 318 353 378 400 414 424 440 C91116 273 295 323 341 352 369 375 397 C91117 284 314 346 369 388 406 421 431 C91118 234 275 305 333 352 372 386 399 C91119 274 311 349 380 407 430 443 460 C91120 271 301 329 357 384 404 354 291 GROUP: FEMALE 1 - 0 PPM C91121 157 178 195 222 243 257 259 271 287 290 C91122 185 216 238 263 282 299 298 309 318 317 C91123 150 167 195 206 228 241 245 258 254 262 1240 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C91124 164 192 216 232 251 268 275 278 290 288 C91125 147 165 182 192 199 210 228 226 234 244 C91126 155 181 204 224 234 250 258 257 C91127 166 191 203 229 241 255 259 268 280 286 C91128 182 204 242 258 277 307 308 323 341 343 C91129 189 210 243 274 296 306 320 330 349 357 C91130 170 203 217 230 239 253 271 269 274 282 C91131 154 169 181 199 213 222 228 234 246 250 C91132 143 160 177 197 212 226 240 243 259 263 C91133 177 194 216 241 246 266 278 291 297 298 C91134 170 201 220 241 252 266 272 281 290 294 C91135 156 179 198 212 C91136 175 206 228 251 267 274 296 302 306 320 C91137 159 167 186 200 203 211 223 229 237 237 C91138 148 172 186 191 191 202 213 218 217 226 C91139 154 187 200 222 233 254 255 275 283 289 C91140 182 209 241 263 283 307 325 326 C91141 167 186 196 222 234 246 252 266 277 282 C91142 183 213 239 268 284 307 319 328 343 347 C91143 139 157 171 198 C91144 169 186 197 215 225 231 235 246 253 257 C91145 194 216 239 248 259 274 285 286 C91146 157 184 214 227 240 261 276 282 285 298 C91147 177 202 225 232 252 257 265 270 276 286 C91148 162 180 195 218 229 237 241 254 263 269 C91149 138 149 164 184 192 199 208 218 230 236 C91150 169 189 203 226 241 247 252 260 269 279 C91151 172 201 221 237 262 295 304 325 323 340 C91152 172 195 219 238 242 260 265 276 289 293 C91153 171 205 237 247 279 297 306 312 315 331 C91154 180 209 237 251 269 285 285 291 309 322 1241 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C91155 172 188 216 233 248 256 272 272 283 294 C91156 170 194 222 237 251 258 277 279 272 278 C91157 164 179 189 210 220 228 228 234 249 253 C91158 156 182 203 222 232 249 262 266 271 287 C91159 165 171 201 205 215 218 234 235 236 248 C91160 160 177 198 215 222 238 243 249 258 259 C91161 152 176 199 213 240 255 268 283 279 299 C91162 148 158 188 205 219 233 246 251 C91163 170 202 226 250 262 270 291 286 C91164 186 208 235 249 263 286 280 297 307 316 C91165 175 202 228 244 C91166 176 206 225 251 262 277 291 293 300 310 C91167 148 175 191 211 228 239 242 251 270 273 C91168 149 172 194 216 231 243 248 259 270 272 C91169 162 181 210 219 C91170 173 209 241 263 279 298 322 327 343 352 C91171 149 166 194 211 C91172 159 188 211 230 254 267 277 278 289 296 C91173 159 186 211 224 235 242 252 257 261 271 C91174 163 178 198 204 217 224 233 242 252 255 C91175 191 218 250 270 292 296 323 329 343 355 C91176 169 200 238 256 280 289 308 319 331 353 C91177 169 183 202 216 219 228 231 243 252 254 C91178 176 198 224 235 239 261 269 266 278 288 C91179 185 214 227 256 268 285 289 297 307 311 C91180 158 181 211 227 240 254 265 270 286 277 C91181 171 206 243 265 273 295 309 310 314 321 C91182 170 192 227 252 257 278 291 295 309 320 C91183 171 197 215 238 263 274 285 293 306 313 C91184 148 171 202 222 250 269 279 282 295 307 C91185 173 198 217 237 252 263 282 291 300 317 1242 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C91186 173 194 214 239 250 261 265 277 296 300 C91187 165 197 223 241 257 277 290 294 304 319 C91188 164 173 176 196 211 220 232 239 243 251 C91189 133 150 164 180 187 199 207 210 217 227 C91190 173 192 197 227 234 240 244 245 253 260 GROUP: FEMALE 2 - 3 PPM C91191 181 206 228 244 259 276 285 294 304 329 C91192 135 158 185 199 217 223 238 239 246 258 C91193 153 168 176 197 211 222 227 236 249 259 C91194 162 181 206 230 241 247 268 273 283 290 C91195 182 205 234 259 274 278 293 305 312 309 C91196 155 172 191 214 219 225 233 238 244 249 C91197 164 185 208 227 245 249 271 282 284 293 C91198 181 207 227 251 C91199 145 168 191 214 224 238 247 259 273 277 C91200 184 195 197 219 226 232 239 234 245 243 C91201 153 178 199 222 239 249 261 265 269 270 C91202 187 209 250 265 282 292 298 299 312 319 C91203 170 189 209 231 246 269 272 276 289 288 C91204 164 177 198 215 223 239 250 250 264 266 C91205 137 156 171 189 C91206 164 184 208 232 248 264 271 277 294 300 C91207 158 187 213 230 249 261 272 273 280 292 C91208 156 185 212 229 239 256 271 268 275 285 C91209 167 193 204 226 235 248 266 262 276 277 C91210 157 182 202 220 223 249 255 249 259 263 C91211 169 202 227 238 266 266 281 286 296 305 C91212 159 181 199 222 233 243 251 258 264 261 C91213 161 186 201 226 238 247 250 263 273 276 1243 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91214 167 193 212 241 238 259 284 273 291 296 C91215 152 174 188 197 207 215 226 227 239 236 C91216 170 192 208 228 238 248 254 263 272 280 C91217 154 171 186 207 228 235 239 252 266 269 C91218 166 181 196 202 221 228 238 235 249 262 C91219 171 183 193 210 218 218 230 240 242 244 C91220 155 180 206 229 244 257 273 269 284 288 C91221 152 183 215 232 248 255 264 277 287 289 C91222 185 213 240 265 C91223 144 155 169 184 194 204 209 213 217 223 C91224 139 157 172 189 203 210 221 224 235 239 C91225 148 170 195 229 221 238 259 269 259 274 C91226 165 183 199 218 223 241 257 266 284 286 C91227 185 202 211 230 248 253 258 270 276 283 C91228 174 200 224 238 247 263 275 277 284 298 C91229 166 189 202 220 229 239 247 243 258 261 C91230 156 187 211 229 C91231 169 196 224 245 263 274 290 295 305 311 C91232 154 176 187 197 212 216 226 224 235 238 C91233 160 183 196 200 217 229 240 241 250 262 C91234 198 224 264 290 309 325 336 339 353 367 C91235 163 171 194 209 212 217 230 230 236 241 C91236 153 175 184 211 221 228 229 236 243 240 C91237 153 175 205 219 C91238 151 170 187 200 213 221 225 227 239 247 C91239 167 191 204 232 246 257 269 274 284 290 C91240 186 209 230 249 266 274 282 285 301 305 C91241 165 187 210 223 240 256 264 272 286 288 C91242 169 191 211 225 241 247 249 253 262 270 C91243 168 191 211 238 243 257 263 273 282 292 C91244 145 169 188 204 219 223 232 239 252 251 1244 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91245 173 186 200 217 231 227 246 254 268 279 C91246 172 191 204 213 227 237 248 237 245 256 C91247 174 200 223 237 244 255 271 281 298 305 C91248 174 203 226 242 261 267 271 285 293 293 C91249 164 189 216 238 246 264 278 291 305 302 C91250 146 168 186 200 202 212 220 223 245 235 GROUP: FEMALE 3 - 30 PPM C91251 176 194 212 235 C91252 161 189 216 236 248 262 267 276 296 304 C91253 170 180 205 209 227 239 246 242 259 256 C91254 157 170 183 201 215 225 230 237 246 246 C91255 168 171 187 194 202 206 218 224 229 244 C91256 164 178 200 221 232 244 253 257 256 262 C91257 171 188 198 221 225 234 247 244 252 258 C91258 163 185 213 235 237 247 261 264 270 284 C91259 159 183 199 216 236 242 254 259 269 274 C91260 189 215 228 242 252 268 279 279 285 294 C91261 155 170 184 192 213 223 232 238 265 265 C91262 163 179 188 195 205 211 216 215 223 228 C91263 162 189 201 224 235 247 257 261 271 277 C91264 148 171 197 207 225 235 248 251 265 263 C91265 180 195 213 227 242 250 261 263 268 274 C91266 169 191 225 244 259 273 297 291 305 310 C91267 135 150 168 182 190 203 210 213 222 220 C91268 156 172 191 205 217 221 230 239 242 245 C91269 172 197 226 242 253 267 271 270 290 296 C91270 140 160 173 192 203 217 224 233 247 252 C91271 183 199 223 240 246 265 280 282 290 286 C91272 181 200 227 237 1245 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 20 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 30 PPM C91273 146 166 185 206 211 234 237 244 255 261 C91274 165 174 201 206 219 228 237 240 245 252 C91275 174 186 219 234 246 261 276 283 289 301 C91276 151 166 180 202 209 218 224 233 242 247 C91277 147 166 194 211 216 226 239 245 254 259 C91278 176 194 217 233 241 255 263 267 274 275 C91279 188 209 227 256 281 288 301 315 329 334 C91280 157 183 210 227 233 250 254 263 267 277 C91281 163 183 212 224 237 246 258 260 275 275 C91282 166 194 211 244 255 272 288 301 300 320 C91283 152 176 200 221 231 239 259 267 272 274 C91284 162 186 213 230 238 255 263 259 268 280 C91285 181 200 218 229 C91286 158 167 176 177 195 198 199 206 218 220 C91287 152 167 178 195 C91288 190 213 232 248 253 265 269 266 269 273 C91289 149 171 187 210 224 233 238 245 253 254 C91290 175 202 217 236 255 253 260 266 282 282 C91291 167 191 209 228 C91292 168 191 217 229 249 266 268 265 285 291 C91293 154 177 196 220 232 247 258 270 277 282 C91294 161 181 208 222 236 251 251 260 267 272 C91295 152 179 193 223 222 238 233 242 245 258 C91296 159 173 190 193 209 223 228 219 236 235 C91297 168 185 194 226 241 243 240 258 267 274 C91298 179 205 223 236 244 259 270 267 272 277 C91299 147 165 179 192 195 211 217 219 224 227 C91300 153 180 198 219 224 240 254 260 271 268 C91301 146 171 182 207 213 228 238 241 257 269 C91302 191 208 219 238 250 262 264 272 274 275 C91303 171 196 219 241 259 266 280 292 304 308 1246 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 21 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 30 PPM C91304 177 209 229 247 263 285 285 294 311 309 C91305 177 200 226 239 258 277 285 290 302 310 C91306 170 191 215 229 236 250 255 254 260 266 C91307 183 206 220 227 254 267 269 271 290 295 C91308 167 187 213 231 250 253 268 276 280 289 C91309 164 192 213 243 251 273 283 301 303 312 C91310 157 180 202 216 219 241 250 253 253 262 GROUP: FEMALE 4 - 100 PPM C91311 158 160 168 182 186 198 209 217 218 226 C91312 160 169 184 193 201 209 215 216 223 231 C91313 155 177 202 220 223 231 241 250 254 259 C91314 155 175 190 201 209 216 226 228 229 232 C91315 153 164 176 189 194 200 206 214 222 229 C91316 157 170 193 211 C91317 164 189 212 233 C91318 169 188 208 220 227 237 240 243 246 248 C91319 140 155 180 195 217 227 236 243 244 253 C91320 182 195 203 210 222 232 236 233 236 238 C91321 164 173 203 219 235 249 250 262 255 267 C91322 171 178 201 219 228 226 241 251 257 261 C91323 167 187 198 212 219 229 235 247 245 248 C91324 153 176 196 204 222 231 235 232 243 249 C91325 175 190 206 217 227 237 241 252 253 262 C91326 141 152 161 177 187 195 195 207 217 216 C91327 158 171 190 203 216 225 227 231 241 243 C91328 165 184 203 212 222 232 237 246 251 259 C91329 146 170 186 203 216 222 233 234 246 249 C91330 156 177 192 209 219 218 230 232 240 244 C91331 159 176 196 219 221 241 246 262 260 268 1247 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 22 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91332 157 174 193 212 217 228 230 233 229 229 C91333 160 174 191 204 C91334 150 175 201 216 231 242 251 261 261 261 C91335 154 171 188 204 214 219 228 235 239 244 C91336 187 200 214 221 230 234 233 236 240 236 C91337 181 205 223 241 253 264 273 270 277 286 C91338 150 161 174 192 202 210 214 217 215 215 C91339 162 177 196 214 224 232 243 241 253 255 C91340 170 196 229 250 266 280 287 295 296 298 C91341 175 191 208 228 242 253 260 270 273 281 C91342 176 192 212 223 228 239 246 247 251 255 C91343 167 179 200 207 220 227 239 244 246 248 C91344 188 202 217 228 241 247 242 255 260 259 C91345 165 173 193 206 C91346 166 180 210 230 242 258 260 275 279 281 C91347 173 194 212 224 234 241 244 246 253 263 C91348 159 178 197 212 225 223 230 235 233 233 C91349 168 189 211 216 219 236 240 246 246 262 C91350 152 168 183 197 C91351 186 198 225 236 252 260 280 283 292 299 C91352 183 207 227 249 260 269 280 288 291 290 C91353 167 179 200 212 222 232 228 233 237 240 C91354 164 180 196 207 220 231 227 233 241 241 C91355 146 166 187 199 216 228 233 240 249 258 C91356 174 191 213 233 254 262 258 268 278 274 C91357 152 161 172 188 197 205 202 210 214 224 C91358 185 196 224 239 256 264 259 266 272 268 C91359 160 176 194 212 220 227 222 234 237 244 C91360 154 170 185 195 203 213 214 223 228 227 C91361 178 193 217 234 243 254 258 268 264 278 C91362 193 213 227 237 242 254 251 256 258 262 1248 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 23 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91363 169 190 216 241 253 256 258 270 278 274 C91364 149 163 177 194 206 221 222 228 235 237 C91365 174 180 188 192 198 201 198 205 208 221 C91366 164 192 197 218 235 237 247 261 271 272 C91367 186 213 233 255 261 272 275 279 290 286 C91368 165 184 202 215 220 232 235 245 245 246 C91369 163 178 193 206 213 228 230 239 245 258 C91370 153 166 179 194 196 205 213 216 220 227 C91371 170 187 211 235 250 266 262 281 281 290 C91372 162 180 202 223 235 247 247 255 262 259 C91373 163 180 193 199 211 222 226 227 237 245 C91374 174 197 219 239 249 258 259 267 268 273 C91375 157 179 201 212 225 236 237 244 247 257 C91376 158 176 192 209 218 229 234 244 251 245 C91377 151 173 192 209 220 229 225 232 241 250 C91378 168 185 201 215 226 234 236 245 249 247 C91379 147 164 186 204 215 226 230 236 237 241 C91380 180 205 225 245 255 266 264 278 283 284 GROUP: FEMALE 5 - 300 PPM C91381 159 166 179 190 195 204 210 215 C91382 152 156 159 170 171 177 171 182 C91383 155 163 174 187 191 203 202 204 C91384 167 171 184 189 188 200 204 207 C91385 158 167 180 193 194 204 203 209 C91386 158 162 172 179 196 200 202 216 C91387 166 169 171 178 185 188 190 193 C91388 177 185 195 205 212 219 217 226 C91389 152 154 166 163 176 180 185 193 C91390 147 159 165 178 190 194 195 206 1249 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 24 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 300 PPM C91391 164 176 189 195 205 213 210 219 C91392 159 176 184 189 191 206 211 217 C91393 150 152 144 160 154 160 161 173 C91394 181 185 191 198 198 200 205 214 C91395 143 152 165 172 178 189 190 189 C91396 184 198 212 216 225 224 231 241 C91397 146 152 159 174 179 190 195 209 C91398 158 174 182 197 209 212 213 219 C91399 175 185 194 204 208 212 211 224 C91400 183 196 205 218 226 231 232 233 C91401 142 158 170 173 174 178 178 182 C91402 148 155 161 167 174 177 176 183 C91403 169 187 196 206 210 217 212 218 C91404 151 160 173 182 191 196 204 207 C91405 158 166 176 186 195 204 208 217 C91406 170 172 179 190 190 198 203 208 C91407 157 163 172 183 189 200 202 210 C91408 173 191 205 216 225 233 235 249 C91409 174 175 176 184 179 197 193 193 C91410 167 162 178 184 189 184 189 200 C91411 160 177 197 210 213 221 228 234 C91412 164 176 183 193 188 202 211 207 C91413 159 176 194 198 207 212 216 219 C91414 168 177 189 202 209 224 230 234 C91415 183 177 181 184 182 196 195 194 C91416 190 201 215 224 229 234 239 248 C91417 161 177 189 195 199 198 204 207 C91418 177 178 189 193 199 205 203 213 C91419 169 182 189 197 209 220 227 236 C91420 155 169 185 195 201 207 210 224 C91421 140 151 163 172 175 191 195 203 1250 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 25 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 300 PPM C91422 177 179 186 197 198 199 202 211 C91423 170 179 198 207 C91424 173 169 178 193 196 204 210 213 C91425 174 180 184 195 190 195 204 214 C91426 146 152 150 157 157 163 171 177 C91427 172 192 207 216 C91428 160 175 184 198 207 219 218 224 C91429 194 209 222 226 234 242 241 251 C91430 149 156 167 180 C91431 155 170 178 182 185 192 197 209 C91432 187 190 204 204 215 220 217 222 C91433 178 188 193 201 199 201 200 212 C91434 159 170 179 184 192 188 192 198 C91435 171 180 191 205 212 222 223 232 C91436 159 154 170 176 182 184 191 203 C91437 161 178 191 205 216 224 229 243 C91438 143 159 167 179 187 195 193 204 C91439 162 181 191 199 209 216 210 213 C91440 171 186 198 204 209 215 214 231 C91441 156 164 175 181 194 198 193 213 C91442 164 174 180 193 194 202 205 210 C91443 184 183 195 209 C91444 152 166 172 185 194 201 201 207 C91445 166 166 174 179 185 186 194 203 C91446 141 144 153 158 164 176 184 192 C91447 162 173 181 191 192 202 203 217 C91448 158 167 178 187 C91449 170 183 193 199 206 210 214 223 C91450 175 177 183 191 201 190 193 197 1251 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 26 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 100 PPM C91451 158 167 189 202 208 224 225 230 238 235 C91452 168 184 206 219 234 247 249 258 272 275 C91453 154 168 193 207 218 226 239 248 256 254 C91454 161 178 198 205 213 221 222 235 236 237 C91455 158 173 191 206 215 219 226 224 235 242 C91456 160 172 187 200 206 210 220 225 229 230 C91457 152 178 202 219 227 240 254 257 258 268 C91458 154 174 192 204 212 224 225 222 237 243 C91459 189 208 232 243 252 262 267 266 267 275 C91460 142 157 178 194 196 196 200 197 200 195 C91461 169 187 209 223 234 244 253 257 265 265 C91462 156 171 197 221 229 239 247 252 263 271 C91463 189 200 225 229 237 248 249 256 258 260 C91464 147 160 181 194 204 214 225 225 226 225 C91465 149 165 182 189 201 212 220 215 225 228 C91466 179 205 228 253 268 270 264 279 279 297 C91467 162 177 199 207 218 224 237 244 245 247 C91468 167 178 187 196 196 211 215 203 219 209 C91469 163 178 198 214 218 227 234 244 247 246 C91470 184 200 230 248 251 266 280 279 293 288 C91471 143 161 184 196 206 220 223 226 238 240 C91472 179 196 220 224 230 244 249 256 256 265 C91473 160 176 191 204 208 220 227 229 227 235 C91474 176 198 216 234 247 256 268 269 283 287 C91475 162 182 208 217 234 248 251 264 268 277 C91476 173 193 206 216 229 239 249 257 258 263 C91477 155 176 190 201 213 223 227 236 242 243 C91478 184 207 230 235 241 250 258 259 261 268 C91479 166 190 215 224 233 249 254 260 264 271 C91480 159 176 196 210 214 225 230 241 243 244 C91481 147 158 177 194 205 217 226 226 232 235 1252 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 100 PPM C91482 153 167 189 204 217 228 232 239 250 252 C91483 170 196 216 227 237 248 252 252 257 267 C91484 185 195 212 229 228 245 247 243 256 257 C91485 159 172 189 206 216 221 228 233 244 244 C91486 173 190 212 218 234 242 247 249 261 263 C91487 158 179 194 209 220 228 235 238 249 253 C91488 202 227 241 252 264 271 274 276 285 C91489 167 176 194 203 217 225 234 227 238 251 C91490 157 180 194 209 220 227 231 234 241 245 GROUP: FEMALE 7 - 300 PPM C91491 156 165 184 195 201 203 207 211 C91492 155 165 179 191 194 205 207 209 C91493 159 163 175 180 188 198 203 205 C91494 157 168 176 185 189 197 201 202 C91495 163 174 186 194 194 200 201 210 C91496 147 164 180 184 186 195 197 205 C91497 168 180 192 205 211 219 225 226 C91498 154 157 174 183 186 185 201 200 C91499 155 166 174 184 189 188 192 193 C91500 179 188 200 208 202 208 208 210 C91501 183 185 182 198 196 203 210 209 C91502 173 186 192 208 211 221 222 229 C91503 189 199 215 221 229 243 241 246 C91504 162 167 171 186 186 193 202 204 C91505 161 169 180 192 193 204 213 213 C91506 172 181 193 201 210 221 222 226 C91507 148 164 176 180 193 197 195 200 C91508 172 184 200 209 212 219 219 223 C91509 165 179 196 202 208 213 212 212 1253 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 28 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 7 - 300 PPM C91510 166 182 200 210 219 226 230 232 C91511 164 181 184 194 203 209 207 213 C91512 170 176 191 199 199 204 214 213 C91513 165 177 191 201 206 210 205 213 C91514 167 187 197 205 209 221 222 225 C91515 156 174 181 190 194 202 209 214 C91516 159 171 182 192 197 202 207 207 C91517 141 158 169 175 174 183 191 197 C91518 158 175 192 201 214 217 224 233 C91519 172 183 183 185 183 187 185 178 C91520 152 165 177 184 195 203 211 213 C91521 163 175 185 200 197 210 210 209 C91522 160 179 187 196 199 201 199 201 C91523 142 160 170 179 189 193 202 208 C91524 181 189 203 216 219 224 225 226 C91525 164 180 189 196 199 204 209 212 C91526 178 194 201 208 209 214 218 226 C91527 186 191 205 213 221 225 227 241 C91528 134 142 151 162 166 167 174 173 C91529 167 190 200 216 222 226 225 232 C91530 164 176 189 196 203 207 217 219 1254 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 29 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C90711 505 516 524 526 529 540 545 570 586 606 C90712 503 515 522 501 513 528 529 559 586 601 C90713 563 578 582 590 596 608 611 638 658 673 C90714 509 521 531 544 546 552 552 576 595 601 C90715 539 546 550 565 575 592 603 619 653 676 C90716 492 503 508 519 511 525 543 567 594 611 C90720 591 600 614 624 633 645 660 686 723 745 C90721 625 646 666 694 715 725 764 813 852 890 C90722 477 486 492 502 501 514 524 552 571 601 C90723 577 558 550 582 593 607 618 642 668 691 C90724 498 511 522 535 541 550 563 586 616 642 C90726 509 518 527 537 543 553 568 596 621 631 C90727 621 636 635 650 655 666 675 699 727 738 C90728 523 531 555 560 569 567 584 625 655 686 C90729 543 553 557 564 579 590 597 616 639 651 C90730 534 546 555 577 588 601 609 641 682 714 C90731 588 597 616 634 C90733 517 529 543 557 562 569 580 611 626 641 C90734 635 656 671 682 692 698 715 751 781 814 C90735 479 491 498 505 507 512 524 544 564 574 C90736 524 538 542 558 566 577 587 617 652 667 C90738 485 497 507 519 519 526 540 570 594 607 C90739 506 521 539 547 556 566 577 608 636 645 C90740 526 544 551 561 567 580 597 639 665 684 C90741 487 503 515 529 534 542 555 582 615 621 C90742 516 522 526 536 547 555 571 591 610 629 C90743 577 588 605 611 623 634 646 696 724 746 C90744 524 534 547 556 565 571 587 609 640 667 C90745 474 472 487 497 498 505 519 553 575 589 C90746 589 597 553 585 C90747 498 503 516 523 536 546 552 583 596 598 C90748 528 533 541 547 1255 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 30 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C90749 465 473 481 484 497 507 514 541 559 567 C90750 500 507 510 522 527 532 544 569 591 606 C90751 499 513 520 530 540 542 558 579 604 618 C90752 610 625 640 650 656 667 681 696 719 720 C90754 551 563 574 579 591 596 604 638 663 689 C90755 610 634 643 657 667 686 695 746 789 803 C90757 584 599 608 617 613 632 642 671 696 715 C90758 534 551 561 564 570 575 586 620 643 678 C90759 511 522 539 540 540 554 562 577 595 609 C90760 521 538 561 573 573 583 601 639 672 700 C90761 520 538 547 555 556 567 576 607 631 648 C90762 479 491 506 512 522 528 539 562 585 599 C90763 575 585 602 600 611 621 626 659 676 684 C90764 503 518 527 536 548 551 557 578 589 612 C90765 602 619 630 640 646 656 666 693 712 739 C90766 516 526 533 547 545 556 562 607 637 640 C90767 481 496 510 515 524 539 546 581 617 648 C90768 468 480 496 499 C90770 452 469 475 484 494 501 513 541 569 587 C90771 513 513 520 525 534 538 553 574 588 598 C90772 529 542 560 565 559 574 588 625 650 678 C90773 548 555 577 585 590 600 609 649 679 711 C90774 511 519 531 538 544 549 562 582 596 609 C90776 582 595 610 624 635 647 663 690 730 754 C90777 605 613 632 640 653 661 674 706 729 757 C90778 503 512 516 520 520 526 528 543 558 567 C90779 516 522 541 544 560 570 590 613 648 675 C90780 572 585 604 617 1256 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 31 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 3 PPM C90781 525 548 563 577 588 606 605 635 657 673 C90782 508 522 533 532 544 557 567 592 620 657 C90783 463 475 477 485 489 500 506 520 534 546 C90784 495 509 512 526 531 536 545 565 573 580 C90785 533 542 561 567 574 590 600 624 655 670 C90786 505 518 527 528 537 545 547 564 598 596 C90787 547 566 581 583 594 609 615 645 669 675 C90788 540 553 566 572 581 590 608 637 660 694 C90790 577 592 605 609 615 625 632 673 714 722 C90791 461 473 479 481 479 495 501 526 552 557 C90792 540 554 563 568 573 571 547 600 599 636 C90793 504 517 533 537 543 555 563 587 608 624 C90794 557 562 577 590 600 617 627 661 695 681 C90795 522 535 541 550 558 562 577 608 634 627 C90796 522 532 462 510 529 546 557 585 610 637 C90797 527 546 557 569 C90798 526 541 546 548 553 568 578 605 627 661 C90799 482 499 507 514 518 524 531 561 579 574 C90800 576 585 600 609 617 621 631 654 674 693 C90801 527 544 550 565 574 581 584 612 643 640 C90802 458 465 473 479 481 483 498 526 555 560 C90803 569 581 592 597 607 623 635 664 694 714 C90804 629 641 645 659 656 675 675 696 706 730 C90805 548 558 562 577 592 600 615 653 681 680 C90806 447 453 459 464 465 465 476 489 503 496 C90807 547 553 566 568 C90808 574 580 592 608 618 628 640 677 712 731 C90809 505 507 525 531 538 542 553 583 590 601 C90810 564 584 587 598 610 621 632 657 694 719 C90811 553 558 558 568 580 592 601 621 644 662 C90812 540 548 564 568 578 582 605 626 640 659 1257 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 32 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 3 PPM C90815 551 559 570 560 576 589 592 622 636 647 C90816 570 574 594 602 607 621 633 665 691 702 C90818 529 541 552 558 C90819 565 578 589 586 589 607 621 649 676 693 C90820 547 555 570 580 575 589 603 633 655 687 C90821 608 625 638 644 651 659 676 709 736 757 C90822 455 468 471 472 484 497 511 540 561 579 C90823 460 474 490 503 516 536 547 571 599 640 C90824 523 532 543 543 558 567 579 606 625 648 C90825 563 576 591 597 602 623 634 663 700 709 C90826 561 578 594 601 607 617 632 666 700 731 C90827 538 563 574 585 581 594 606 655 682 714 C90828 583 594 611 621 624 635 649 687 728 748 C90829 592 615 625 631 649 657 681 715 758 789 C90830 544 557 566 568 576 585 596 622 650 666 C90831 555 575 595 610 C90832 557 572 579 587 590 605 610 645 670 690 C90835 510 525 526 535 537 549 556 585 618 633 C90836 505 525 538 552 C90837 477 488 496 504 511 516 525 552 573 590 C90838 626 647 659 670 680 695 712 757 800 843 C90839 620 640 657 662 680 688 705 744 785 814 C90840 567 583 599 609 616 631 643 689 733 743 GROUP: MALE 3 - 30 PPM C90841 495 509 522 532 542 546 557 571 599 620 C90842 416 426 429 434 441 441 449 469 483 490 C90843 542 552 557 560 C90844 492 502 518 532 541 549 555 594 637 653 C90845 527 540 551 555 563 571 577 611 631 654 1258 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 33 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90847 495 511 518 525 528 534 546 560 587 583 C90848 527 540 555 557 572 586 606 639 670 702 C90849 562 578 592 607 614 617 628 654 677 693 C90850 477 486 494 497 507 509 521 533 555 574 C90851 493 498 505 511 512 518 529 548 560 578 C90852 515 529 541 551 C90854 525 536 545 549 564 568 574 607 623 648 C90855 559 569 582 596 603 613 623 643 654 652 C90856 528 547 553 558 555 562 567 598 624 635 C90857 528 540 549 555 557 567 576 597 617 626 C90858 443 453 463 462 474 474 490 516 537 545 C90859 529 546 559 571 577 580 586 616 638 630 C90860 425 437 446 448 447 455 460 473 485 479 C90861 500 522 533 539 544 552 563 587 604 619 C90862 398 400 409 417 421 427 440 473 487 499 C90863 645 657 667 685 C90864 484 498 504 514 518 528 535 556 572 577 C90865 471 476 482 489 495 494 506 522 543 548 C90867 478 486 493 501 502 504 512 532 537 547 C90868 569 589 603 612 624 631 646 667 694 721 C90869 540 555 561 574 575 574 580 611 624 652 C90870 557 559 568 576 583 592 583 633 663 674 C90871 493 505 514 518 526 542 542 560 567 565 C90872 508 519 530 536 545 551 561 586 604 625 C90873 569 574 574 579 589 603 607 629 644 665 C90874 606 617 640 649 649 660 668 693 716 745 C90875 496 507 521 538 544 557 575 597 623 639 C90876 610 632 641 653 651 657 664 697 741 754 C90877 473 483 492 495 C90878 452 460 468 484 489 490 503 520 529 538 C90879 522 527 536 545 555 554 570 607 637 669 1259 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 34 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90880 582 593 596 611 C90881 548 562 575 587 593 601 616 642 670 695 C90882 524 534 539 548 557 564 576 599 616 631 C90883 527 539 549 530 559 566 572 590 604 624 C90884 483 490 495 497 502 506 514 536 537 551 C90885 491 511 519 523 537 542 556 599 634 653 C90886 553 567 578 587 594 603 616 654 677 681 C90887 528 539 548 561 570 586 603 636 669 704 C90888 502 512 517 521 501 517 538 557 573 575 C90889 491 501 503 513 511 515 523 553 570 580 C90892 402 410 420 424 420 430 440 462 479 501 C90893 477 496 503 510 518 523 530 561 576 604 C90894 486 503 507 518 534 538 557 589 608 634 C90895 482 494 512 514 522 533 545 565 584 613 C90896 484 502 510 517 526 534 538 554 572 581 C90897 573 593 607 619 599 613 633 673 710 732 C90898 505 513 521 526 533 542 549 572 597 608 C90899 440 446 452 466 466 474 478 494 515 528 C90900 507 519 527 534 540 550 562 575 591 610 GROUP: MALE 4 - 100 PPM C90901 439 449 458 461 463 474 475 501 519 539 C90902 452 463 475 484 488 503 510 534 551 577 C90903 431 442 452 457 459 469 482 498 516 538 C90904 464 476 478 488 492 497 498 512 530 535 C90905 444 449 455 468 C90906 488 503 519 522 533 539 546 576 597 631 C90907 470 480 483 492 C90908 507 515 532 539 545 553 559 587 599 637 C90909 560 572 590 604 609 617 631 657 695 713 1260 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 35 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90910 474 485 490 500 504 516 525 536 559 568 C90911 496 508 513 521 520 534 536 554 568 582 C90912 531 541 555 557 560 568 573 589 594 627 C90913 473 488 495 497 496 506 505 512 539 557 C90914 543 555 566 582 589 597 606 626 646 667 C90915 534 548 558 562 570 583 588 606 637 663 C90916 433 451 457 465 469 480 485 507 530 537 C90917 507 523 531 528 C90918 527 541 546 559 562 573 574 597 605 629 C90919 529 539 545 545 551 554 557 582 599 620 C90920 491 499 510 520 526 531 544 569 580 602 C90921 518 532 547 553 C90922 478 488 498 509 512 524 527 557 572 583 C90923 492 504 511 521 530 534 538 559 564 585 C90924 512 534 543 544 552 557 568 613 605 597 C90925 445 455 463 471 476 487 499 518 533 550 C90926 536 551 557 565 573 586 597 631 646 678 C90927 498 507 517 522 530 537 547 565 591 607 C90928 466 472 475 479 485 493 509 514 531 549 C90929 429 438 442 413 442 459 471 496 524 533 C90930 481 494 504 510 519 527 534 542 551 571 C90931 503 515 526 532 539 546 551 579 587 605 C90932 427 438 447 456 460 470 471 496 513 523 C90933 534 542 551 558 565 576 589 619 630 650 C90934 448 457 462 466 465 483 488 500 512 528 C90936 464 477 486 493 503 516 524 528 556 577 C90937 465 474 474 474 476 475 472 500 502 520 C90938 444 453 457 465 464 473 483 507 526 553 C90940 556 575 578 592 600 603 614 644 659 673 C90941 523 536 536 550 552 562 571 585 616 638 C90943 465 480 488 488 495 497 494 459 515 537 1261 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 36 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90944 430 433 440 448 446 456 463 482 491 498 C90945 447 454 468 473 480 489 492 499 521 524 C90946 495 500 507 519 518 530 530 555 568 588 C90947 461 476 485 493 492 499 518 537 551 572 C90948 467 475 484 487 491 501 512 523 549 569 C90949 339 305 266 C90950 515 527 535 550 556 559 573 592 598 618 C90951 489 506 509 515 527 528 541 558 564 588 C90952 572 573 585 596 598 604 622 645 676 706 C90953 490 497 511 523 516 525 536 542 547 562 C90954 446 461 462 468 471 476 489 507 526 541 C90955 491 502 505 517 521 533 536 557 566 577 C90956 481 504 514 518 525 535 542 575 594 608 C90957 448 471 470 479 480 492 508 490 510 526 C90958 490 498 493 506 515 531 545 570 586 600 C90959 498 496 504 509 522 528 541 569 590 607 C90960 495 500 507 516 C90962 531 546 550 554 558 570 586 604 629 639 C90963 540 548 557 563 574 579 587 605 613 621 C90964 591 599 611 613 614 629 636 666 690 715 C90965 418 422 431 392 414 420 431 442 458 462 C90967 491 505 516 522 528 535 541 561 574 589 C90968 472 483 503 509 509 520 526 552 565 589 C90969 554 569 584 598 605 617 618 641 655 681 C90970 445 458 466 474 486 487 495 517 538 553 GROUP: MALE 6 - 100 PPM C91041 559 568 582 588 597 599 618 629 642 648 C91042 450 466 484 494 497 501 516 539 549 585 C91043 469 484 496 506 509 513 519 531 552 575 1262 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 37 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 100 PPM C91044 481 491 504 509 511 521 526 543 561 583 C91045 538 552 570 580 585 581 601 628 645 664 C91047 425 438 457 461 462 469 480 482 478 496 C91048 487 497 509 522 526 530 539 561 597 614 C91049 548 566 572 578 585 608 619 655 694 725 C91050 513 528 544 558 565 565 558 586 596 614 C91051 529 543 555 564 571 578 583 616 630 650 C91052 577 591 617 616 628 639 653 698 736 779 C91053 464 474 485 495 500 505 515 538 563 570 C91054 513 513 529 534 536 544 546 583 604 628 C91055 551 569 584 592 600 616 624 660 674 702 C91056 460 478 500 505 519 526 541 561 585 608 C91057 513 526 541 538 550 560 574 582 601 622 C91058 479 490 496 496 507 519 521 535 549 560 C91059 503 516 520 532 537 542 545 559 571 599 C91060 436 449 452 454 459 470 477 495 507 525 C91061 521 526 543 555 569 572 585 599 606 612 C91062 491 506 514 522 532 542 554 562 577 598 C91063 393 401 410 414 418 425 427 439 442 456 C91064 490 503 515 527 537 547 562 581 598 618 C91065 457 468 482 488 489 502 512 511 532 543 C91066 513 524 535 543 550 557 562 587 597 614 C91067 500 514 521 531 536 545 557 581 596 618 C91068 198 195 C91069 488 501 520 527 535 544 557 602 623 659 C91070 476 481 489 494 500 510 517 532 546 554 C91071 562 578 586 596 603 614 624 638 646 662 C91072 436 445 447 452 454 461 474 478 492 505 C91073 548 563 585 595 608 622 643 660 680 703 C91074 444 452 457 467 473 474 479 506 508 526 C91075 472 477 489 497 497 511 524 550 572 601 1263 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 38 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 100 PPM C91076 465 487 500 510 511 525 530 560 581 608 C91077 474 483 493 499 499 512 527 544 519 543 C91078 458 470 477 482 489 491 503 525 542 545 C91079 504 517 522 523 527 532 540 557 575 596 C91080 480 496 501 508 508 513 523 544 561 574 GROUP: FEMALE 1 - 0 PPM C91121 286 302 310 310 312 321 332 344 340 361 C91122 331 346 334 328 329 335 337 349 374 394 C91123 262 272 273 278 275 277 281 300 303 319 C91124 291 298 304 298 294 303 310 311 321 321 C91125 250 253 251 257 265 263 264 280 297 313 C91127 290 290 299 295 298 304 310 319 332 339 C91128 343 362 362 362 373 372 387 402 435 445 C91129 367 368 387 402 C91130 288 282 283 290 291 296 290 301 302 317 C91131 248 258 262 265 267 269 275 286 288 290 C91132 273 275 284 291 290 295 303 320 345 361 C91133 299 315 321 321 319 330 332 346 365 358 C91134 302 308 301 315 325 322 335 355 380 395 C91136 322 329 328 338 340 358 349 373 388 405 C91137 247 249 247 246 254 262 259 259 268 275 C91138 232 238 240 252 257 258 257 263 268 283 C91139 298 297 310 306 316 315 332 331 346 363 C91141 278 295 298 298 293 304 315 323 342 348 C91142 361 368 361 377 380 383 387 408 428 430 C91144 252 260 265 262 257 263 265 270 277 281 C91146 308 309 313 321 324 329 330 346 359 370 C91147 291 295 307 313 318 325 332 348 371 391 C91148 274 284 289 286 280 288 295 313 311 322 1264 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 39 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C91149 234 239 250 253 258 265 266 277 297 304 C91150 279 292 294 298 299 305 312 319 336 346 C91151 335 360 363 370 371 382 386 408 429 450 C91152 289 296 300 294 302 304 312 322 331 333 C91153 326 343 349 347 355 354 365 371 426 456 C91154 308 322 333 324 335 334 344 355 362 387 C91155 294 297 300 304 C91156 275 292 302 303 309 324 331 356 378 408 C91157 249 258 259 255 253 260 265 282 293 291 C91158 294 296 293 306 314 319 319 332 342 366 C91159 254 257 258 268 277 275 274 291 297 309 C91160 270 264 276 280 C91161 303 312 307 317 317 320 334 340 362 369 C91164 325 323 340 335 339 344 349 361 379 438 C91166 319 325 324 326 327 329 333 344 353 363 C91167 273 287 294 291 290 290 300 328 352 349 C91168 272 280 286 286 286 293 297 310 314 323 C91170 368 369 364 365 369 384 384 416 439 455 C91172 295 293 301 307 303 304 307 315 308 323 C91173 275 282 288 291 296 297 300 307 310 329 C91174 264 275 283 284 C91175 374 385 387 385 399 406 414 432 444 458 C91176 356 374 370 364 364 371 375 406 413 431 C91177 261 265 267 275 287 298 299 291 307 333 C91178 292 287 300 300 293 307 313 337 327 344 C91179 316 326 328 328 326 336 332 344 360 383 C91180 295 295 300 307 317 328 337 360 392 416 C91181 326 327 330 332 C91182 331 329 347 349 346 349 360 378 398 427 C91183 315 325 324 326 327 328 340 347 360 356 C91184 314 321 330 328 333 343 347 364 383 399 1265 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 40 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C91185 327 332 344 341 346 362 357 382 412 423 C91186 300 309 315 311 312 317 325 336 346 347 C91187 333 333 342 345 349 346 347 361 375 391 C91188 260 262 269 266 275 284 283 290 306 307 C91189 234 235 243 244 247 250 252 257 261 271 C91190 268 270 269 270 271 278 282 293 308 311 GROUP: FEMALE 2 - 3 PPM C91191 326 328 329 326 336 337 331 371 394 418 C91192 269 271 268 276 C91193 259 261 274 275 271 276 279 295 304 311 C91194 302 310 307 309 313 320 323 328 360 377 C91195 321 325 323 321 326 334 323 342 372 380 C91196 249 255 268 268 265 273 280 293 317 336 C91197 308 301 304 307 307 312 319 334 354 370 C91199 288 290 291 295 300 308 307 318 321 333 C91200 262 265 264 268 270 272 272 290 294 303 C91201 276 278 286 293 292 294 302 319 322 335 C91202 318 326 332 331 327 341 349 357 381 403 C91203 292 301 304 313 321 330 340 352 379 397 C91204 277 280 290 295 281 299 305 327 337 349 C91206 309 318 320 324 325 338 337 364 404 421 C91207 296 297 295 301 304 310 308 320 325 342 C91208 277 285 288 288 291 292 299 314 324 332 C91209 288 283 281 291 298 291 301 304 319 330 C91210 266 271 276 276 277 280 287 300 319 337 C91211 318 308 317 311 321 323 328 347 359 378 C91212 269 273 280 286 292 292 295 308 323 335 C91213 276 287 290 288 302 307 309 318 339 356 C91214 302 307 301 307 314 327 331 330 366 371 1266 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 41 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91215 237 242 247 250 251 252 262 267 284 301 C91216 278 283 288 289 290 300 306 309 312 324 C91217 270 275 285 282 283 289 295 304 304 317 C91218 252 254 265 267 270 273 286 294 302 317 C91219 248 252 258 252 263 275 275 286 312 355 C91220 294 303 308 304 310 319 322 325 352 358 C91221 290 298 307 315 308 314 320 341 353 355 C91223 229 232 237 241 C91224 241 238 251 251 257 260 268 272 287 288 C91225 273 272 271 281 282 284 297 307 316 315 C91226 297 299 306 318 320 327 334 356 380 407 C91227 285 293 299 298 301 312 314 317 329 340 C91228 295 298 298 310 302 305 318 315 333 340 C91229 258 266 274 289 281 289 286 304 304 310 C91231 321 330 332 333 337 347 353 368 382 405 C91232 240 242 250 254 254 255 266 278 286 288 C91233 260 263 274 276 C91234 367 360 370 380 385 391 405 425 441 450 C91235 242 247 250 249 250 259 262 270 278 273 C91236 239 245 254 247 245 246 248 262 279 280 C91238 248 253 259 260 261 260 267 268 291 300 C91239 297 298 305 304 309 311 313 333 343 354 C91240 305 295 288 290 309 305 309 319 334 340 C91241 299 303 309 314 C91242 272 273 278 283 282 279 287 297 299 307 C91243 300 297 308 304 317 319 329 333 361 373 C91244 253 260 266 265 267 264 279 288 306 310 C91245 289 290 292 299 309 305 326 339 374 376 C91246 248 247 255 267 264 266 279 291 293 314 C91247 312 317 322 327 335 339 348 371 401 421 C91248 305 317 318 310 1267 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 42 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91249 321 324 333 333 329 342 346 372 382 410 C91250 247 239 246 247 251 245 265 269 271 271 GROUP: FEMALE 3 - 30 PPM C91252 309 316 310 314 317 319 322 339 348 375 C91253 262 269 267 276 273 275 278 297 300 323 C91254 243 252 257 259 253 259 266 272 276 288 C91255 246 262 271 279 275 279 291 291 309 316 C91256 266 270 274 278 279 278 285 296 306 316 C91257 253 256 259 262 261 263 270 277 288 300 C91258 284 286 292 294 294 297 294 314 329 337 C91259 281 283 294 295 297 301 308 321 336 358 C91260 297 307 312 312 315 326 329 349 368 383 C91261 254 251 264 269 268 266 278 294 303 315 C91262 222 224 228 229 229 233 236 252 257 265 C91263 281 292 297 303 308 317 325 350 375 394 C91264 266 259 275 272 272 281 283 299 302 299 C91265 278 280 285 280 287 289 298 300 310 321 C91266 312 319 323 319 323 317 319 318 354 367 C91267 224 232 233 232 239 241 239 254 275 275 C91268 252 258 263 260 258 268 271 288 309 330 C91269 299 297 308 311 313 309 323 329 337 372 C91270 263 287 270 267 272 276 287 306 315 314 C91271 295 298 300 304 307 305 313 322 328 328 C91273 269 277 285 286 287 289 294 305 315 326 C91274 257 264 270 269 271 272 286 289 310 334 C91275 300 311 315 317 323 328 333 345 376 393 C91276 241 249 256 249 248 258 263 273 285 292 C91277 259 268 269 272 279 285 284 292 294 302 C91278 276 286 287 285 288 293 296 310 317 327 1268 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 43 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 30 PPM C91279 331 346 354 358 357 367 370 417 453 476 C91280 279 277 282 289 290 300 299 306 322 330 C91281 284 281 294 285 C91282 319 324 330 332 347 345 357 374 390 406 C91283 285 293 292 289 289 290 296 319 338 345 C91284 282 277 279 290 293 291 290 314 323 331 C91286 212 218 226 228 228 237 244 254 262 280 C91288 279 278 278 288 C91289 246 260 265 262 265 269 271 282 297 307 C91290 272 289 294 295 302 310 320 348 364 378 C91292 293 289 295 302 305 316 322 342 351 359 C91293 285 296 303 301 C91294 279 276 280 286 286 289 292 307 315 325 C91295 259 256 264 262 265 271 268 297 298 313 C91296 241 235 239 244 259 270 259 261 261 277 C91297 266 279 288 283 282 296 303 318 332 335 C91298 284 280 283 287 294 294 296 308 299 307 C91299 232 234 240 241 C91300 286 285 288 291 283 299 304 314 340 361 C91301 271 277 277 283 279 289 295 308 333 342 C91302 280 288 285 292 289 290 300 310 320 331 C91303 315 322 329 336 343 356 360 361 381 394 C91304 322 323 331 339 C91305 312 313 316 323 314 328 334 367 367 387 C91306 271 270 266 271 274 274 276 288 295 293 C91307 293 293 299 295 300 302 302 316 331 336 C91308 283 288 289 297 299 308 307 314 326 331 C91309 317 319 318 331 332 336 347 362 377 385 C91310 265 266 270 276 278 278 282 292 308 324 1269 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 44 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91311 223 224 223 224 224 227 232 239 248 259 C91312 230 233 231 238 240 241 246 252 261 260 C91313 257 261 263 262 261 275 269 273 282 290 C91314 237 236 237 239 240 245 247 254 260 270 C91315 237 240 242 243 240 248 251 263 267 275 C91318 247 244 244 248 246 C91319 252 255 262 262 263 270 275 277 288 313 C91320 248 250 253 256 257 261 267 273 285 292 C91321 268 261 267 263 272 272 276 277 288 301 C91322 265 267 265 269 274 275 289 301 C91323 253 255 263 265 273 275 280 292 303 311 C91324 248 248 248 248 251 250 259 258 265 271 C91325 260 263 267 273 274 274 280 283 288 295 C91326 208 213 219 215 203 211 213 223 233 240 C91327 247 250 243 255 252 250 262 270 275 284 C91328 260 259 266 264 269 267 274 270 295 303 C91329 250 258 260 256 C91330 254 249 252 257 256 266 268 271 295 305 C91331 274 281 274 285 282 289 293 311 332 362 C91332 235 238 235 242 237 242 243 249 258 260 C91334 265 266 274 275 277 276 286 289 298 308 C91335 246 250 251 251 257 257 260 267 272 274 C91336 239 245 247 243 247 248 252 258 254 261 C91337 286 289 295 294 C91338 213 214 212 217 215 222 228 231 235 242 C91339 259 260 264 265 266 269 272 282 285 304 C91340 302 301 297 298 299 299 300 304 317 321 C91341 277 278 280 282 280 285 286 298 308 319 C91342 259 259 267 267 265 269 275 285 289 292 C91343 246 251 257 258 258 265 262 275 282 295 C91344 264 267 265 260 266 268 274 278 276 279 1270 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 45 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91346 287 292 302 302 308 307 318 326 331 349 C91347 267 261 264 273 270 270 277 282 290 298 C91348 239 237 240 239 236 241 247 251 258 259 C91349 260 261 252 265 262 261 271 279 282 299 C91351 301 308 306 313 313 316 324 325 332 328 C91352 296 294 290 299 299 305 311 322 327 336 C91353 239 247 244 248 253 255 253 260 271 275 C91354 239 238 244 244 240 240 246 245 248 252 C91355 270 276 281 283 C91356 276 279 275 274 271 274 275 293 308 311 C91357 218 224 224 229 227 228 231 240 242 249 C91358 283 284 280 288 286 291 299 311 322 328 C91359 243 245 247 249 249 258 258 271 281 282 C91360 230 233 237 239 240 242 247 247 259 263 C91361 272 269 276 284 278 281 284 293 301 304 C91362 262 263 263 267 273 274 273 282 301 311 C91363 282 288 294 300 300 303 305 310 326 322 C91364 239 239 248 243 254 247 251 255 260 268 C91365 211 216 215 220 219 228 225 225 232 243 C91366 268 276 272 279 284 281 286 293 302 314 C91367 297 296 291 295 293 302 299 311 328 340 C91368 251 249 253 255 255 257 262 264 273 278 C91369 268 266 269 274 281 282 287 295 304 311 C91370 235 232 237 235 C91371 293 282 290 298 297 308 295 314 327 337 C91372 265 270 275 273 270 278 284 296 298 309 C91373 240 240 236 245 250 252 262 272 298 322 C91374 275 275 278 282 279 282 286 292 299 303 C91375 259 259 257 266 268 267 266 276 281 286 C91376 248 255 256 253 257 258 264 266 271 280 C91377 252 249 257 261 259 261 265 276 292 306 1271 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 46 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91378 245 251 246 249 251 256 258 262 272 276 C91379 247 249 251 252 C91380 289 286 297 293 303 297 304 314 315 320 GROUP: FEMALE 6 - 100 PPM C91451 242 246 251 253 254 253 262 263 278 284 C91452 274 279 284 289 289 290 292 298 316 323 C91453 260 263 269 265 272 276 280 286 299 304 C91454 237 242 247 247 249 251 258 260 272 280 C91455 246 247 246 250 247 250 255 257 272 273 C91456 238 242 247 246 250 255 261 263 279 286 C91457 264 267 270 276 280 280 290 299 310 317 C91458 245 240 244 254 250 254 256 264 275 280 C91459 275 284 284 288 288 291 288 297 307 319 C91460 199 211 214 214 216 221 215 233 239 241 C91461 267 282 279 279 289 293 308 331 354 382 C91462 268 275 279 282 290 295 297 303 316 329 C91463 266 265 266 261 264 271 276 274 282 285 C91464 228 234 228 232 234 241 234 241 250 261 C91465 232 229 233 241 240 241 249 249 272 290 C91466 289 299 289 301 298 302 304 311 331 335 C91467 251 256 258 255 260 264 264 272 272 283 C91468 221 213 220 226 230 224 234 252 263 269 C91469 253 254 254 259 261 264 266 269 276 279 C91470 300 296 301 306 313 315 315 321 334 334 C91471 248 251 249 259 259 263 265 274 287 300 C91472 268 273 268 271 276 283 281 285 297 308 C91473 237 242 239 244 248 248 250 255 263 267 C91474 286 291 294 299 302 304 306 314 318 316 C91475 273 281 286 289 285 292 298 313 326 332 1272 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 47 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 100 PPM C91476 267 267 267 271 275 274 279 292 309 314 C91477 247 257 261 263 268 269 274 280 297 311 C91478 272 272 274 279 280 281 286 293 310 319 C91479 273 279 278 278 285 284 288 300 311 330 C91480 252 255 260 260 257 258 261 269 277 281 C91481 235 231 233 231 230 243 242 245 254 257 C91482 249 255 259 261 265 268 270 276 288 295 C91483 263 264 270 270 272 272 280 289 289 297 C91484 256 253 259 264 267 266 267 261 280 304 C91485 243 251 251 247 239 251 257 264 276 279 C91486 264 263 271 276 283 282 283 286 296 299 C91487 250 257 257 257 258 256 261 266 291 302 C91488 285 289 290 291 295 300 302 311 315 324 C91489 246 245 254 260 260 261 270 279 289 301 C91490 248 252 258 259 257 259 261 272 279 287 1273 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 48 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C90711 621 633 644 656 673 687 693 711 720 730 C90712 614 617 630 613 642 652 659 688 671 691 C90713 693 708 725 744 778 787 801 821 825 838 C90714 599 613 621 649 666 681 C90715 693 716 718 734 755 771 789 804 821 845 C90716 639 658 671 694 712 736 745 765 772 797 C90720 765 791 806 838 862 888 888 923 935 961 C90721 921 953 823 C90722 620 639 642 671 685 706 719 740 750 772 C90723 716 730 725 756 789 798 795 794 C90724 666 678 695 720 735 755 766 795 827 848 C90726 639 646 650 665 678 692 695 690 711 707 C90727 748 765 760 785 793 807 806 823 837 842 C90728 709 733 745 785 820 838 856 879 908 927 C90729 672 680 677 703 712 736 755 773 796 812 C90730 729 764 769 804 826 839 836 796 643 532 C90733 653 670 676 689 703 718 722 655 700 731 C90734 843 861 876 910 922 941 950 978 919 926 C90735 595 613 626 645 664 676 689 703 709 732 C90736 678 696 711 734 747 782 795 816 805 719 C90738 618 643 662 687 695 705 708 718 729 735 C90739 648 656 673 699 700 724 745 755 767 776 C90740 699 721 728 759 780 804 810 769 614 C90741 636 676 694 713 731 762 776 778 800 807 C90742 643 644 651 666 671 693 715 717 744 764 C90743 778 808 822 849 878 910 922 942 962 966 C90744 688 702 709 742 766 784 794 813 822 835 C90745 588 611 620 647 647 668 686 713 714 726 C90747 621 633 646 652 668 682 688 705 705 674 C90749 598 609 613 630 651 662 674 697 711 729 C90750 621 640 629 647 677 693 710 735 735 763 C90751 637 653 666 699 725 751 1274 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 49 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C90752 733 737 745 783 790 811 C90754 710 738 749 789 807 837 C90755 841 869 869 919 966 985 994 1013 1035 1044 C90757 732 746 767 807 822 854 884 911 939 808 C90758 680 704 707 738 756 786 786 800 822 821 C90759 607 628 627 644 659 679 674 681 692 704 C90760 722 741 749 775 806 823 843 857 868 891 C90761 637 664 656 683 702 721 721 739 752 770 C90762 602 613 591 606 638 657 654 677 689 703 C90763 687 688 699 709 724 743 771 783 796 807 C90764 625 570 604 632 655 674 679 694 700 718 C90765 748 768 776 803 812 847 860 877 878 886 C90766 677 694 701 726 751 766 703 688 C90767 658 637 660 681 719 745 C90770 600 603 607 640 642 662 674 679 685 695 C90771 604 618 633 654 665 678 677 693 709 715 C90772 697 706 713 746 771 789 777 717 C90773 737 756 771 808 842 875 901 951 910 C90774 616 623 627 644 657 673 669 685 695 573 C90776 780 794 808 833 844 881 917 946 962 983 C90777 766 797 814 845 834 877 890 895 906 915 C90778 577 593 596 619 641 649 651 666 668 675 C90779 686 701 712 747 773 782 784 803 833 833 GROUP: MALE 2 - 3 PPM C90781 683 703 706 725 749 763 774 782 792 800 C90782 665 674 684 708 716 716 732 748 773 776 C90783 563 567 579 593 605 618 629 630 639 648 C90784 594 599 612 634 637 644 658 666 676 692 C90785 685 700 717 736 755 770 781 804 808 813 1275 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 50 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 3 PPM C90786 605 620 626 647 666 673 678 694 701 702 C90787 675 691 707 719 749 783 803 830 851 873 C90788 707 735 755 784 803 832 848 876 899 914 C90790 734 755 761 800 826 832 860 870 906 879 C90791 566 586 597 616 631 652 661 680 690 703 C90792 650 666 672 696 693 700 503 C90793 641 656 664 695 717 735 726 751 758 768 C90794 531 583 684 743 799 829 858 883 897 901 C90795 664 684 698 726 729 687 514 C90796 647 665 672 697 705 711 669 C90798 671 691 705 739 753 784 790 808 818 843 C90799 586 594 593 612 633 651 658 671 686 691 C90800 704 712 726 746 760 780 794 813 828 830 C90801 669 686 699 739 672 746 760 779 797 812 C90802 587 605 609 631 654 667 675 689 673 700 C90803 735 760 775 797 819 837 841 851 823 796 C90804 744 754 767 800 804 832 853 856 866 863 C90805 704 723 729 744 777 800 634 480 C90806 617 C90808 756 761 767 796 818 839 863 880 717 517 C90809 609 623 634 638 654 648 667 675 688 696 C90810 736 762 781 834 862 884 899 937 941 965 C90811 666 683 698 704 639 724 747 752 773 795 C90812 675 690 708 727 737 757 747 752 775 778 C90815 641 648 643 674 682 704 723 732 745 749 C90816 741 773 779 809 837 859 878 909 925 942 C90819 721 749 779 819 851 861 889 920 931 963 C90820 690 701 720 752 775 788 810 828 845 729 C90821 780 805 810 848 877 903 919 941 950 961 C90822 606 630 645 645 657 681 687 706 710 715 C90823 642 666 679 704 722 741 757 757 760 763 1276 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 51 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 3 PPM C90824 659 684 694 724 755 778 785 821 834 851 C90825 723 723 723 743 767 790 809 838 835 856 C90826 731 742 748 772 798 807 832 847 850 854 C90827 736 757 764 794 834 854 901 923 950 C90828 768 774 791 824 868 883 859 814 774 755 C90829 818 851 871 923 950 981 991 1016 1022 1050 C90830 676 682 697 708 726 744 753 759 772 794 C90832 702 720 747 768 801 818 832 851 867 878 C90835 638 642 639 664 689 720 722 730 735 763 C90837 604 615 600 616 625 608 549 505 486 455 C90838 872 902 891 942 986 1014 1040 1072 1094 1111 C90839 825 860 884 916 943 971 983 997 1008 1043 C90840 783 816 829 866 887 908 950 986 1010 970 GROUP: MALE 3 - 30 PPM C90841 649 677 682 700 713 739 756 772 791 797 C90842 510 512 511 527 538 561 574 591 590 604 C90844 684 698 716 738 768 797 776 815 841 867 C90845 667 680 687 710 736 756 781 805 821 852 C90847 611 622 623 662 683 701 720 742 751 772 C90848 744 752 790 825 859 899 918 961 969 993 C90849 729 736 738 780 820 832 859 872 863 790 C90850 591 602 605 620 641 650 673 694 716 725 C90851 594 598 608 632 652 668 695 711 731 744 C90854 662 667 681 697 728 744 744 772 771 780 C90855 677 671 678 695 707 728 744 761 775 792 C90856 663 679 695 721 739 757 764 782 801 826 C90857 649 664 664 673 691 704 715 720 725 730 C90858 565 584 589 619 646 666 681 689 705 711 C90859 639 653 655 684 719 752 736 671 552 554 1277 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 52 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90860 490 493 491 504 517 526 540 550 552 564 C90861 635 651 646 670 683 700 721 742 702 580 C90862 523 534 543 570 598 615 633 641 548 439 C90864 598 616 624 633 641 651 682 668 693 707 C90865 562 569 579 596 607 624 632 639 650 664 C90867 551 552 553 559 566 569 573 576 573 584 C90868 741 762 780 802 825 858 882 869 869 846 C90869 673 691 710 751 776 800 830 846 857 847 C90870 695 715 726 755 775 795 810 829 836 825 C90871 586 596 598 611 636 643 662 669 674 688 C90872 626 631 642 670 699 716 731 732 735 746 C90873 669 683 678 703 721 733 738 747 751 722 C90874 767 775 786 816 828 861 728 815 692 C90875 651 668 676 692 720 748 727 758 778 804 C90876 767 791 797 850 882 891 C90878 537 532 543 556 566 582 C90879 680 694 702 723 751 755 757 769 782 777 C90881 705 728 744 781 797 821 827 854 874 894 C90882 659 686 693 713 740 771 794 838 858 869 C90883 595 609 618 639 638 638 647 657 648 667 C90884 548 561 568 587 586 593 598 616 620 625 C90885 661 677 688 724 743 759 781 795 820 823 C90886 716 735 755 790 817 845 848 885 872 878 C90887 739 756 775 796 830 860 896 922 940 704 C90888 589 595 599 619 621 620 636 637 646 676 C90889 598 604 618 637 655 671 671 699 718 728 C90892 510 512 530 554 579 592 605 620 626 613 C90893 627 650 643 666 704 733 757 776 793 803 C90894 651 664 665 696 732 749 770 780 791 788 C90895 618 631 632 658 673 692 696 718 726 741 C90896 587 599 601 631 667 694 699 697 623 512 1278 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90897 736 753 752 789 803 825 840 861 871 874 C90898 612 623 627 641 647 656 673 563 C90899 539 544 548 571 583 602 622 650 671 671 C90900 615 624 619 649 653 668 683 692 702 708 GROUP: MALE 4 - 100 PPM C90901 562 569 580 601 617 650 681 697 723 749 C90902 586 590 593 619 641 657 C90903 543 542 553 570 588 609 624 643 658 668 C90904 537 546 556 557 555 566 578 581 593 598 C90906 646 663 684 702 726 728 745 766 761 782 C90908 641 652 663 667 673 699 704 704 716 730 C90909 723 720 738 771 801 828 C90910 586 598 615 636 657 671 680 692 702 709 C90911 587 592 601 621 640 660 675 695 687 704 C90912 627 641 646 653 675 686 692 699 613 559 C90913 580 594 593 609 627 640 C90914 689 698 704 724 742 761 773 791 815 836 C90915 683 703 717 741 759 773 791 806 814 833 C90916 560 568 581 602 632 661 675 668 681 695 C90918 640 656 656 688 693 714 C90919 617 624 621 648 657 672 657 652 648 650 C90920 607 619 636 629 654 673 697 712 732 730 C90922 602 614 617 636 647 633 594 477 C90923 589 608 605 620 642 647 661 673 687 704 C90924 596 587 590 616 620 646 C90925 553 577 587 606 612 629 658 685 703 717 C90926 687 705 710 740 772 765 790 799 806 821 C90927 567 580 589 592 599 618 636 662 671 679 C90928 558 565 567 572 578 591 612 628 642 645 1279 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 54 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90929 545 548 554 560 570 569 618 622 658 C90930 568 565 561 576 584 612 628 638 645 662 C90931 611 630 635 665 677 681 703 726 739 739 C90932 520 540 544 561 572 589 C90933 641 641 655 672 664 677 694 710 721 711 C90934 539 538 554 564 565 587 594 616 631 651 C90936 577 578 572 595 616 624 647 664 654 663 C90937 524 535 547 582 599 616 641 638 655 654 C90938 566 579 592 611 631 650 663 667 683 546 C90940 693 701 717 746 765 805 813 735 C90941 641 659 686 698 734 775 806 837 867 887 C90943 547 550 578 588 589 618 638 624 649 666 C90944 517 523 531 542 555 568 569 584 590 594 C90945 540 556 560 C90946 586 558 599 605 621 647 657 650 649 643 C90947 586 600 612 633 651 669 680 691 707 713 C90948 587 590 601 612 617 633 649 663 651 661 C90950 636 648 679 701 722 735 C90951 593 601 606 624 629 658 C90952 729 746 762 801 816 798 696 C90953 560 576 581 602 624 645 667 673 679 699 C90954 564 575 585 614 632 652 652 640 588 444 C90955 591 602 612 634 650 679 701 713 725 735 C90956 620 623 631 650 658 677 688 713 713 699 C90957 542 561 548 553 575 605 C90958 611 634 636 643 676 685 700 664 688 705 C90959 619 628 641 669 677 710 736 749 767 770 C90962 663 678 695 709 743 767 790 804 773 635 C90963 630 655 672 697 710 740 770 769 654 529 C90964 729 731 735 754 774 784 777 807 770 670 C90965 439 477 472 490 494 513 512 526 506 524 1280 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 55 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90967 608 631 647 673 681 701 C90968 609 614 629 647 669 677 685 700 717 530 C90969 698 708 715 773 786 811 837 862 876 893 C90970 562 574 578 610 623 643 657 679 688 709 GROUP: MALE 6 - 100 PPM C91041 667 679 700 738 754 779 749 443 C91042 618 627 646 662 682 691 721 747 762 782 C91043 594 612 626 648 662 676 700 743 762 787 C91044 600 603 602 617 645 657 612 463 C91045 662 684 706 733 705 C91047 490 496 509 509 515 530 543 568 590 615 C91048 620 634 661 680 699 724 750 775 776 791 C91049 728 737 602 C91050 649 636 648 667 682 718 742 785 785 812 C91051 653 668 678 683 704 742 772 807 811 830 C91052 782 803 822 835 875 918 966 945 753 C91053 581 592 610 628 645 653 678 692 692 680 C91054 642 671 697 727 750 788 815 864 898 936 C91055 716 745 753 794 806 830 873 919 950 983 C91056 618 633 642 653 675 675 694 699 714 735 C91057 629 628 629 652 567 630 640 659 677 671 C91058 572 574 582 600 616 628 637 659 690 708 C91059 605 620 633 654 671 704 742 768 789 826 C91060 539 549 567 590 608 620 646 680 696 724 C91061 616 621 628 642 642 660 675 688 719 738 C91062 596 611 630 651 670 686 706 734 754 768 C91063 467 477 476 486 498 504 519 535 548 548 C91064 619 632 635 673 687 711 731 757 785 813 C91065 542 554 564 566 571 591 601 625 607 625 1281 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 56 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 100 PPM C91066 624 632 642 656 670 685 707 735 751 734 C91067 640 656 675 691 713 731 757 780 802 824 C91069 682 686 719 757 786 821 850 895 954 990 C91070 558 564 559 569 585 596 607 629 647 670 C91071 667 675 687 712 736 751 769 810 841 875 C91072 517 527 544 563 584 597 619 639 650 667 C91073 695 714 727 756 784 823 862 910 928 949 C91074 537 532 548 564 588 601 621 648 661 672 C91075 627 642 666 689 731 757 777 805 818 842 C91076 615 617 632 632 643 670 682 719 744 771 C91077 557 580 604 635 653 688 712 729 737 747 C91078 556 568 561 527 570 610 616 656 673 699 C91079 613 612 615 628 645 671 693 730 747 757 C91080 571 582 594 600 612 636 669 698 696 729 GROUP: FEMALE 1 - 0 PPM C91121 379 374 385 403 409 435 C91122 388 395 396 435 455 479 C91123 326 341 356 372 392 398 413 422 435 442 C91124 335 333 342 359 376 389 C91125 312 328 340 357 376 392 399 421 440 456 C91127 355 358 379 400 427 453 458 486 500 514 C91128 470 467 499 525 547 565 581 590 621 659 C91130 326 335 355 395 381 410 425 435 451 471 C91131 299 299 301 309 316 310 318 329 331 336 C91132 391 404 421 447 466 480 492 520 514 525 C91133 372 401 388 403 437 455 C91134 388 420 446 484 515 534 568 587 596 472 C91136 418 443 437 448 466 503 513 540 545 564 C91137 286 293 309 317 339 331 323 344 360 382 1282 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 57 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C91138 291 296 298 301 307 298 306 326 323 324 C91139 380 404 412 431 446 463 478 355 C91141 354 354 376 413 429 443 444 440 440 442 C91142 459 474 483 518 535 532 537 531 539 542 C91144 290 280 283 293 298 302 305 305 308 309 C91146 395 411 435 457 471 488 514 490 521 470 C91147 411 424 440 456 470 492 509 518 538 536 C91148 339 347 350 361 383 397 413 436 441 456 C91149 316 327 343 359 364 387 389 390 390 393 C91150 352 382 398 420 448 458 471 479 463 467 C91151 478 497 534 573 593 625 635 646 655 560 C91152 341 360 378 398 411 426 C91153 488 511 553 582 617 651 671 694 710 734 C91154 413 403 423 440 457 465 488 505 518 528 C91156 426 419 417 472 495 508 524 548 564 572 C91157 302 312 318 325 332 336 345 351 355 363 C91158 359 406 428 475 485 492 506 528 549 565 C91159 333 346 356 368 386 400 425 442 448 466 C91161 370 399 431 440 461 472 405 244 C91164 442 456 489 525 540 547 579 606 622 659 C91166 370 374 380 406 415 423 434 444 460 478 C91167 385 383 416 437 466 491 501 527 524 560 C91168 337 339 350 369 396 411 415 422 419 428 C91170 463 479 505 536 544 570 581 566 544 538 C91172 325 335 336 351 365 379 381 397 397 415 C91173 338 330 349 376 382 364 275 C91175 474 473 497 539 543 559 579 589 595 609 C91176 446 463 471 501 512 523 555 571 588 603 C91177 336 336 374 364 388 407 421 446 475 506 C91178 362 367 385 416 425 442 438 442 435 453 C91179 396 407 424 461 1283 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 58 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C91180 427 435 437 462 495 528 522 571 C91182 423 457 472 510 522 541 580 603 632 666 C91183 387 389 398 428 442 457 481 490 504 522 C91184 409 430 465 483 509 543 571 613 634 651 C91185 450 440 493 525 554 563 558 451 302 C91186 357 351 360 386 402 417 431 452 458 471 C91187 406 413 435 461 486 515 516 492 440 374 C91188 326 340 348 372 388 397 419 437 447 467 C91189 275 276 282 286 298 303 306 318 318 327 C91190 323 327 327 346 357 362 375 382 375 390 GROUP: FEMALE 2 - 3 PPM C91191 440 433 470 507 493 521 536 519 562 600 C91193 315 320 328 359 372 381 394 413 423 442 C91194 395 399 403 425 447 451 424 405 415 436 C91195 388 409 420 443 464 473 472 487 506 543 C91196 359 357 378 417 450 464 473 502 537 571 C91197 391 412 426 443 472 490 485 505 496 521 C91199 343 352 357 372 392 412 415 439 454 470 C91200 315 327 348 340 363 369 380 387 402 426 C91201 348 364 373 392 408 410 414 432 455 647 C91202 413 435 448 481 496 522 547 570 592 576 C91203 415 430 455 465 478 508 514 535 556 561 C91204 371 391 407 436 458 477 453 372 C91206 435 442 449 447 468 489 491 513 473 403 C91207 354 358 362 372 389 395 382 403 401 401 C91208 338 347 354 368 378 399 409 416 413 365 C91209 339 343 352 359 376 393 393 400 397 394 C91210 349 365 379 403 422 431 446 477 484 493 C91211 389 398 431 456 467 480 495 504 523 551 1284 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 59 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91212 341 354 372 385 393 416 432 452 361 C91213 382 380 409 436 456 477 481 499 514 528 C91214 364 395 416 427 439 452 462 471 485 505 C91215 320 328 350 349 348 344 C91216 331 330 338 350 354 357 361 361 358 358 C91217 319 319 330 348 355 372 385 408 424 C91218 315 320 338 351 382 364 377 399 410 427 C91219 336 329 379 386 397 415 414 433 436 450 C91220 373 374 396 412 434 448 454 483 494 501 C91221 384 387 399 422 439 452 456 480 494 498 C91224 300 302 322 340 337 344 370 386 391 411 C91225 338 341 340 357 352 357 373 389 399 404 C91226 433 438 433 429 434 446 462 484 501 509 C91227 356 365 372 380 402 421 427 434 453 456 C91228 347 352 359 377 390 393 398 418 418 440 C91229 309 310 321 325 337 344 349 358 363 380 C91231 422 427 429 470 478 501 519 530 555 583 C91232 292 299 305 314 322 318 336 356 368 375 C91234 515 503 533 586 632 648 684 719 679 C91235 291 295 306 316 329 340 343 356 369 369 C91236 291 299 309 315 321 338 348 368 372 375 C91238 322 340 350 371 396 408 420 437 343 C91239 371 377 382 394 399 406 418 C91240 340 352 354 351 363 388 382 401 374 389 C91242 308 312 313 325 348 333 354 358 369 384 C91243 394 408 421 428 445 451 455 467 480 494 C91244 319 312 315 335 340 350 367 386 382 C91245 401 391 415 442 454 474 483 516 520 530 C91246 327 338 349 362 374 395 414 421 430 447 C91247 428 436 455 489 511 522 527 548 484 402 C91249 416 441 461 490 515 544 565 593 615 631 1285 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 60 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91250 281 288 294 304 320 345 365 397 437 473 GROUP: FEMALE 3 - 30 PPM C91252 386 373 390 428 452 475 495 497 520 537 C91253 340 354 361 383 404 417 420 440 454 470 C91254 289 299 299 309 322 334 339 357 373 385 C91255 333 335 342 354 365 372 378 394 390 392 C91256 320 337 351 356 362 372 385 394 397 407 C91257 315 321 327 344 358 366 381 398 406 395 C91258 354 360 376 397 411 414 415 436 440 455 C91259 378 386 402 432 449 432 361 318 C91260 418 433 449 475 491 512 529 552 539 446 C91261 321 321 333 360 368 385 401 408 416 424 C91262 267 273 293 298 316 325 338 355 363 380 C91263 414 424 436 461 472 487 500 506 440 391 C91264 320 315 332 341 353 364 381 385 392 402 C91265 334 336 354 356 374 391 399 418 436 435 C91266 379 388 397 416 427 430 448 467 482 496 C91267 297 312 333 355 365 380 378 393 422 C91268 345 363 376 394 415 438 440 450 459 477 C91269 389 397 429 437 461 468 486 497 526 569 C91270 321 341 356 387 333 340 296 C91271 335 350 362 382 398 416 440 477 491 525 C91273 337 343 356 371 385 389 407 409 418 429 C91274 349 361 382 402 407 432 441 469 465 470 C91275 407 411 429 463 485 484 462 412 333 310 C91276 302 297 312 316 318 323 328 334 336 342 C91277 311 316 326 336 338 346 360 371 384 418 C91278 331 341 346 363 373 380 389 389 398 402 C91279 492 504 515 534 552 582 571 598 611 609 1286 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 61 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 30 PPM C91280 342 349 364 374 386 406 422 441 456 467 C91282 424 439 477 506 442 276 C91283 350 379 389 417 422 437 465 485 498 497 C91284 343 334 353 374 367 390 402 419 434 459 C91286 281 278 304 297 307 323 339 337 343 362 C91289 311 315 333 352 360 340 305 281 279 265 C91290 379 378 393 401 390 369 311 C91292 382 372 394 421 441 473 507 532 551 585 C91294 352 331 337 369 361 369 371 398 407 416 C91295 340 320 327 370 377 390 408 424 442 460 C91296 274 274 283 294 295 302 303 311 314 322 C91297 348 368 377 386 386 397 405 431 411 421 C91298 311 316 317 320 332 319 314 338 304 319 C91300 372 378 392 411 418 422 432 400 356 335 C91301 357 365 392 418 437 461 485 501 523 549 C91302 345 357 369 383 399 404 371 359 308 274 C91303 422 434 428 470 477 501 518 537 557 584 C91305 415 417 449 482 499 525 533 561 570 588 C91306 320 320 327 332 342 345 361 372 379 386 C91307 357 365 368 376 391 399 412 423 433 447 C91308 334 340 359 373 387 403 392 390 385 327 C91309 400 413 426 441 457 477 482 513 477 C91310 323 327 359 379 395 404 419 431 445 450 GROUP: FEMALE 4 - 100 PPM C91311 271 276 287 284 288 298 303 302 C91312 267 272 272 275 282 284 283 288 287 289 C91313 296 306 322 335 348 357 375 385 388 390 C91314 285 291 295 302 316 323 326 335 342 354 C91315 283 291 299 315 332 341 354 364 374 382 1287 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 62 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91319 319 325 340 340 353 356 359 364 363 334 C91320 307 315 327 343 360 375 394 404 426 433 C91321 310 312 332 346 366 384 404 420 427 436 C91323 323 330 349 354 369 373 C91324 272 275 271 277 284 286 295 291 300 301 C91325 302 304 314 313 313 302 315 328 337 358 C91326 243 252 257 264 262 267 278 283 288 297 C91327 291 289 299 295 297 304 318 326 329 339 C91328 303 302 313 317 327 334 333 344 C91330 317 323 337 351 364 372 393 418 428 447 C91331 390 406 422 438 460 481 504 509 502 481 C91332 263 266 274 274 276 284 289 295 294 310 C91334 319 334 357 374 392 412 422 430 431 425 C91335 290 287 304 296 309 313 320 329 341 344 C91336 265 250 277 279 284 292 299 302 305 308 C91338 244 243 253 241 269 265 263 260 268 289 C91339 314 323 334 348 355 365 375 386 403 400 C91340 344 340 348 353 358 383 388 398 409 416 C91341 323 328 337 335 347 354 367 371 376 382 C91342 299 306 313 321 324 349 344 353 378 391 C91343 296 293 291 293 298 297 C91344 297 291 296 310 310 316 324 332 333 340 C91346 356 356 386 403 406 418 433 437 439 391 C91347 300 297 304 305 316 317 C91348 266 257 271 281 280 286 C91349 306 306 268 305 307 310 324 C91351 339 342 346 373 373 397 410 443 468 479 C91352 341 352 359 369 391 408 C91353 274 297 303 302 334 344 C91354 257 255 267 272 275 277 285 285 269 269 C91356 300 299 313 322 333 347 385 417 412 430 1288 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 63 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91357 247 250 248 248 250 257 258 264 288 270 C91358 338 341 353 377 387 403 411 430 452 440 C91359 295 267 295 301 311 323 333 340 347 369 C91360 263 268 270 276 279 285 289 301 304 312 C91361 307 306 313 320 320 322 327 329 340 351 C91362 323 329 342 358 376 404 415 433 440 464 C91363 353 355 346 358 361 375 377 399 407 413 C91364 276 271 278 282 295 290 299 305 308 315 C91365 242 243 249 252 259 260 C91366 333 340 351 360 375 400 412 436 449 465 C91367 344 306 329 339 320 333 C91368 274 284 285 281 290 294 C91369 319 325 340 346 359 365 377 402 425 466 C91371 353 366 377 385 399 411 C91372 307 311 323 334 336 343 351 367 371 371 C91373 336 349 359 367 374 379 369 381 377 253 C91374 308 319 330 338 349 375 376 394 407 429 C91375 291 294 300 309 313 317 324 334 335 357 C91376 282 287 291 293 294 306 304 308 315 304 C91377 316 330 333 342 352 367 387 427 455 495 C91378 276 279 290 290 294 285 286 283 291 295 C91380 311 313 321 325 323 317 327 328 325 319 GROUP: FEMALE 6 - 100 PPM C91451 286 288 299 308 312 315 346 369 382 402 C91452 337 346 354 374 381 394 423 470 493 543 C91453 317 326 341 351 358 360 387 423 426 464 C91454 277 284 287 291 293 289 306 335 338 337 C91455 288 288 300 301 314 321 342 366 367 377 C91456 290 293 301 309 309 314 340 371 403 431 1289 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 64 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 100 PPM C91457 325 329 340 348 354 362 379 414 431 454 C91458 287 284 297 302 321 324 340 351 C91459 322 327 321 331 337 334 380 408 443 489 C91460 244 243 246 249 256 255 246 282 C91461 401 419 436 451 472 500 527 577 609 610 C91462 349 347 359 379 386 391 415 432 445 463 C91463 290 301 306 309 315 327 349 386 399 407 C91464 263 269 271 272 279 290 302 324 330 324 C91465 307 313 324 333 338 349 388 408 420 439 C91466 346 353 366 375 390 412 426 465 492 503 C91467 284 291 293 303 310 322 341 359 377 378 C91468 272 280 280 285 270 286 288 298 C91469 287 290 297 303 306 308 322 346 353 320 C91470 349 354 373 382 401 412 434 462 460 297 C91471 304 318 331 346 363 390 410 430 450 446 C91472 314 323 337 347 369 388 416 461 494 514 C91473 273 280 281 290 297 307 314 343 361 393 C91474 319 321 339 337 342 353 367 420 420 448 C91475 343 357 363 385 388 386 386 456 483 528 C91476 322 327 335 344 351 361 374 424 437 454 C91477 322 327 338 348 367 373 392 423 441 450 C91478 332 337 347 360 365 386 418 456 473 505 C91479 343 353 368 390 382 396 430 466 493 507 C91480 291 296 306 313 322 335 340 367 369 383 C91481 267 266 278 303 313 323 341 381 394 354 C91482 312 317 326 333 332 346 359 401 427 453 C91483 310 316 324 330 337 348 349 370 385 398 C91484 296 302 312 323 326 345 348 382 344 263 C91485 279 279 293 295 296 299 318 341 349 365 C91486 300 310 318 324 330 350 361 402 419 446 C91487 310 311 318 326 332 349 386 430 478 496 1290 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 65 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 100 PPM C91488 336 343 352 365 365 373 377 412 433 458 C91489 305 318 337 347 351 368 391 436 464 498 C91490 291 302 314 326 337 361 381 413 417 409 1291 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 66 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: C90711 C90712 C90713 C90715 C90716 C90720 C90722 C90724 C90726 C90727 C90728 C90729 C90733 C90734 C90735 C90738 C90739 C90741 C90742 C90743 C90744 C90745 C90747 C90749 C90750 C90755 C90758 C90759 C90760 C90761 C90762 C90763 MALE 1 - 0 PPM 674 516 704 720 847 850 868 885 815 838 977 987 793 812 872 893 723 700 852 860 948 969 819 846 732 644 915 807 743 745 722 718 783 792 828 840 772 795 978 1004 851 862 709 502 696 636 740 741 784 797 1057 1067 821 834 709 664 878 821 785 765 715 719 807 818 705 838 860 842 978 811 902 691 845 949 845 658 746 715 788 834 797 1019 845 618 731 811 1051 589 538 702 782 836 703 829 854 862 945 830 931 690 843 932 856 633 769 712 796 844 803 1047 759 606 705 835 1042 757 387 670 802 702 683 752 784 879 866 830 956 671 803 926 838 645 761 690 807 873 810 1069 580 653 829 1019 799 802 675 666 685 889 851 962 647 720 931 604 673 691 830 805 809 1074 568 460 841 994 788 807 636 588 915 862 977 660 485 937 685 673 849 654 819 1094 540 854 960 781 555 924 890 974 662 937 689 749 818 1090 511 864 781 434 923 886 981 634 909 712 816 1089 488 856 739 1292 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 67 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C90764 727 718 712 C90765 900 908 932 925 897 907 906 922 930 C90770 708 706 705 647 536 C90771 717 734 723 692 594 545 C90776 1013 1029 1027 1031 1031 1044 1029 C90777 931 902 848 781 C90778 680 686 678 686 670 676 699 704 739 C90779 830 813 796 770 674 459 GROUP: MALE 2 - 3 PPM C90781 815 842 833 849 851 839 837 826 770 C90782 797 813 772 763 768 756 710 709 701 C90783 659 676 679 653 655 672 667 675 686 C90784 705 696 666 639 588 495 C90785 831 813 782 806 805 801 774 693 510 C90786 716 721 723 741 736 719 C90787 885 908 897 915 920 918 941 871 634 C90788 931 947 960 992 987 1010 1033 1059 1070 C90790 896 820 640 C90791 719 732 735 759 774 788 811 813 796 C90793 756 685 C90794 931 902 900 884 857 852 879 839 795 C90798 870 892 907 919 908 923 914 782 C90799 705 709 706 712 705 702 704 709 707 C90800 836 823 789 C90801 832 758 783 792 841 865 890 C90802 714 664 445 C90803 743 709 652 641 616 598 C90804 860 857 857 844 815 778 736 641 538 C90809 691 664 681 661 671 696 717 734 743 1293 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 68 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 3 PPM C90810 C90811 C90812 C90815 C90816 C90819 C90821 C90822 C90823 C90824 C90825 C90826 C90828 C90829 C90830 C90832 C90835 C90837 C90838 C90839 C90840 977 813 784 757 944 1002 990 695 789 845 875 887 738 1080 794 803 764 439 1101 1037 984 1000 824 779 767 883 1012 991 512 786 825 879 886 695 1105 805 850 767 1110 1002 987 979 839 775 774 831 1005 1006 790 684 876 874 1127 797 829 770 1118 866 988 977 838 780 764 655 1026 1004 811 886 896 1147 828 846 757 1126 819 892 990 839 762 742 1031 1023 808 871 888 1172 838 854 740 1108 808 895 999 848 747 741 1061 800 875 1190 842 860 755 1088 725 893 999 856 703 742 1100 784 869 1202 848 856 755 1075 714 880 984 851 623 713 787 1224 857 877 718 1026 873 944 877 524 670 799 1219 822 906 677 963 841 GROUP: MALE 3 - 30 PPM C90841 795 817 823 832 740 750 527 C90842 608 615 625 629 617 605 592 581 592 C90844 851 860 879 852 853 850 835 799 673 C90845 873 780 C90847 772 775 778 727 682 636 520 C90848 986 C90849 660 499 1294 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 69 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90850 752 767 782 783 789 776 672 637 606 C90851 760 765 778 776 781 796 819 831 832 C90854 797 798 812 816 808 818 816 803 775 C90855 797 801 805 817 799 811 821 816 818 C90856 852 856 869 890 899 923 936 946 946 C90857 736 719 704 720 708 711 665 557 C90858 725 717 709 692 682 680 662 646 614 C90860 574 578 580 597 593 600 613 609 609 C90864 714 714 719 734 724 728 739 740 756 C90865 664 679 689 695 708 735 748 750 733 C90867 588 593 599 604 610 618 612 616 619 C90868 667 C90869 878 903 914 914 929 943 928 910 924 C90870 803 675 C90871 706 709 719 734 740 743 755 754 752 C90872 757 773 789 799 735 C90873 640 C90875 807 826 826 835 842 C90879 752 657 448 C90881 917 926 951 950 940 976 1008 1026 1014 C90882 874 899 917 921 936 959 1004 1016 1016 C90883 657 670 674 691 676 679 682 680 653 C90884 633 637 635 633 647 681 697 675 626 C90885 835 841 836 846 825 866 794 788 781 C90886 885 910 840 694 573 C90888 721 689 439 C90889 743 769 751 765 778 822 835 849 859 C90892 578 422 C90893 827 838 835 855 864 892 930 954 944 C90894 805 797 785 806 785 781 751 C90895 752 759 765 780 782 781 1295 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 70 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90897 897 917 914 914 930 953 C90899 682 696 695 702 716 738 748 656 C90900 712 716 716 713 711 709 727 696 699 GROUP: MALE 4 - 100 PPM C90901 700 C90903 687 699 713 712 724 739 746 663 C90904 613 619 631 632 632 621 612 615 601 C90906 801 806 816 826 850 872 864 740 C90908 748 760 743 768 777 785 791 811 833 C90910 722 729 742 751 754 768 791 819 845 C90911 717 716 731 733 733 734 738 749 758 C90912 524 507 429 C90914 861 891 914 938 914 868 777 C90915 851 885 891 901 912 906 C90916 706 712 717 723 730 750 753 749 770 C90919 655 657 C90920 736 763 771 759 754 794 827 813 C90923 729 742 717 552 C90925 732 760 780 792 780 643 C90926 835 854 858 887 853 855 749 684 442 C90927 703 716 742 746 755 769 772 787 801 C90928 662 667 683 607 493 522 481 496 527 C90930 683 688 699 643 597 C90931 742 747 763 744 745 761 710 C90933 736 732 728 743 745 765 792 814 842 C90934 668 675 679 701 704 719 727 692 C90936 695 714 740 746 720 746 735 661 C90937 661 664 653 659 643 630 594 520 C90941 905 848 728 605 1296 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 71 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90943 686 657 650 659 580 C90944 603 609 618 630 636 635 640 654 669 C90946 615 485 C90947 725 730 709 698 698 691 667 617 550 C90948 666 653 640 657 663 653 655 649 655 C90953 724 729 729 750 756 779 780 762 766 C90955 756 748 774 786 790 789 787 787 793 C90956 736 756 789 811 826 841 836 820 630 C90958 748 694 717 709 701 683 C90959 798 785 806 756 C90963 450 C90964 532 C90965 527 538 547 543 545 556 542 557 570 C90968 715 744 762 652 677 659 655 603 645 C90969 894 890 739 653 C90970 729 742 755 771 772 784 799 GROUP: MALE 6 - 100 PPM C91042 C91043 C91047 C91048 C91050 C91051 C91053 C91054 C91055 C91056 C91057 C91058 814 817 633 800 818 852 686 976 1034 754 624 745 830 859 659 712 828 870 690 982 1066 775 759 824 893 649 838 872 662 1005 989 778 784 849 941 664 860 733 1028 769 847 663 863 492 1061 771 690 655 858 1067 777 687 767 1088 777 692 760 1105 773 720 1084 766 1297 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 72 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 100 PPM C91059 870 866 666 C91060 761 787 800 816 812 817 829 831 833 C91061 755 763 773 801 788 C91062 786 780 593 551 405 335 C91063 567 578 595 607 617 570 439 C91064 838 853 866 888 889 891 915 C91065 650 669 685 674 682 708 734 763 759 C91066 687 533 480 417 C91067 838 831 840 857 853 875 836 C91069 1046 1088 1129 C91070 686 687 695 690 682 676 667 643 621 C91071 904 927 949 974 980 998 1014 1000 955 C91072 666 666 669 651 623 577 590 565 500 C91073 989 1005 990 877 919 954 972 993 1027 C91074 708 734 732 703 722 744 759 719 C91075 836 736 C91076 789 800 820 774 778 760 787 778 795 C91077 770 735 713 C91078 703 713 709 700 690 642 C91079 719 683 609 463 C91080 754 769 780 802 819 810 747 GROUP: FEMALE 1 - 0 PPM C91123 453 464 484 502 521 530 533 C91125 482 490 505 526 526 490 C91127 538 540 538 554 553 555 557 530 365 C91128 663 C91130 473 381 C91131 343 351 352 365 361 372 368 374 358 C91132 518 484 493 528 464 1298 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 73 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C91134 332 C91136 581 609 618 582 482 C91137 406 442 451 468 481 494 501 458 C91138 316 322 322 343 348 370 372 400 441 C91141 447 468 C91142 367 C91144 313 317 313 325 326 336 333 336 349 C91146 395 C91147 482 336 C91148 465 480 494 516 523 553 587 623 631 C91149 396 392 406 394 C91150 431 C91151 367 C91153 754 737 716 C91154 529 509 C91156 576 C91157 371 369 372 389 384 393 404 401 422 C91158 572 598 626 658 684 715 C91159 479 490 528 548 541 575 585 586 613 C91164 687 703 714 664 628 563 C91166 491 511 519 540 547 566 587 598 612 C91167 583 604 617 617 611 636 656 684 C91168 433 441 467 C91170 540 512 418 343 262 C91172 430 425 419 411 405 436 458 483 489 C91175 638 650 662 590 C91176 632 637 662 692 707 729 672 602 458 C91177 545 576 595 619 644 652 C91178 453 475 481 483 410 C91182 693 709 600 425 C91183 538 549 562 586 589 605 616 620 645 1299 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 74 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C91184 675 699 700 704 594 C91186 486 484 494 515 502 443 461 C91187 298 C91188 507 505 528 539 545 565 602 609 605 C91189 344 347 355 355 356 324 C91190 405 401 408 407 407 417 437 428 412 GROUP: FEMALE 2 - 3 PPM C91191 629 640 661 661 628 664 553 C91193 463 457 466 458 389 C91194 471 480 483 486 471 442 441 372 C91195 561 573 595 613 611 570 468 C91196 596 614 497 C91197 538 542 546 560 558 578 603 595 620 C91199 492 504 533 534 534 469 377 C91200 422 445 452 458 C91201 474 495 500 500 511 521 492 429 407 C91202 608 637 659 644 C91203 590 C91206 382 C91207 406 420 431 434 426 416 449 438 452 C91208 340 C91209 424 C91210 498 C91211 563 553 562 572 578 596 609 607 603 C91213 488 399 C91214 536 536 545 540 535 571 548 C91216 370 370 372 366 371 380 387 396 403 C91218 448 368 450 361 345 385 332 C91219 459 476 460 365 1300 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 75 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91220 523 536 559 573 561 602 639 667 675 C91221 519 531 548 558 588 622 641 648 628 C91224 426 415 433 444 440 409 368 C91225 422 438 443 C91226 512 426 416 435 C91227 481 495 504 506 513 516 522 538 575 C91228 458 458 477 493 451 392 C91229 396 338 392 362 372 303 C91231 598 610 625 644 642 C91232 391 392 406 396 403 419 420 434 434 C91235 381 404 422 412 430 433 462 477 490 C91236 386 393 409 420 434 446 462 477 472 C91240 415 438 C91242 412 409 427 427 430 449 447 461 474 C91243 516 521 547 562 577 592 612 611 604 C91245 558 570 595 603 587 542 C91246 454 431 431 437 C91247 401 C91249 665 685 705 715 709 563 409 C91250 505 GROUP: FEMALE 3 - 30 PPM C91252 548 554 560 580 577 611 625 630 637 C91253 479 485 496 517 527 527 544 558 577 C91254 382 389 386 398 395 397 421 435 434 C91255 400 C91256 414 420 430 441 439 452 424 420 423 C91257 378 333 283 C91258 462 463 462 326 C91260 326 1301 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 76 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 30 PPM C91261 437 453 468 472 468 435 433 C91262 400 405 407 395 282 C91264 412 404 419 434 430 449 470 477 424 C91265 450 464 466 481 461 363 C91266 515 523 534 545 561 570 524 468 C91268 512 517 547 562 580 601 619 C91269 593 521 410 C91271 548 561 539 443 404 359 C91273 426 424 360 C91276 339 342 340 362 352 393 420 C91278 410 422 427 423 426 432 445 444 448 C91279 602 612 590 583 572 563 569 588 573 C91280 484 492 497 501 505 509 419 C91283 525 517 541 534 386 311 C91284 471 465 481 478 481 499 504 C91286 367 366 378 380 380 398 394 410 415 C91289 227 C91292 610 629 649 666 684 715 634 775 782 C91294 434 468 456 471 472 496 515 516 491 C91295 490 495 519 528 538 562 565 551 491 C91296 335 335 311 256 C91297 421 417 433 429 446 C91298 342 C91300 316 317 311 C91301 570 584 601 623 645 666 687 697 705 C91303 591 607 617 621 610 634 640 645 626 C91305 608 612 617 603 C91306 391 397 413 404 406 418 413 460 C91307 456 464 483 490 503 516 517 474 427 C91310 476 494 511 529 535 534 549 543 558 1302 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 77 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91312 293 293 302 309 298 284 217 C91313 392 392 380 370 C91314 341 C91315 395 405 414 419 421 439 451 470 510 C91320 428 353 C91321 471 473 491 486 459 427 C91324 308 314 327 331 323 332 336 337 348 C91325 366 379 386 406 424 433 424 364 C91326 292 275 237 221 C91327 353 354 367 400 405 469 495 513 532 C91330 474 501 501 418 C91331 465 327 C91332 314 320 327 340 336 341 336 315 335 C91334 425 431 422 422 417 403 315 348 C91335 359 362 369 378 386 409 410 402 424 C91336 320 318 330 337 336 346 345 354 359 C91338 281 297 292 312 310 311 317 330 338 C91339 423 425 438 454 443 404 327 C91340 413 408 409 405 397 403 390 323 318 C91341 392 397 398 415 430 445 460 458 468 C91342 393 405 416 423 427 448 459 462 469 C91344 350 355 369 377 381 394 375 355 C91351 514 536 520 467 454 385 C91354 260 255 247 242 C91356 443 471 523 531 510 486 456 391 316 C91357 292 288 288 301 299 306 325 315 326 C91358 427 C91359 389 399 C91360 319 321 327 335 330 346 352 345 359 C91361 354 365 370 365 364 373 361 360 369 C91362 500 519 535 576 604 644 657 668 675 1303 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 78 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91363 434 434 428 452 446 475 296 C91364 323 312 317 293 271 274 271 276 C91366 485 484 466 402 C91369 484 C91372 388 386 401 400 394 394 408 400 397 C91374 435 439 436 433 405 367 300 C91375 372 385 358 C91376 323 322 331 335 330 332 336 340 354 C91378 296 304 305 312 306 304 297 300 316 C91380 333 324 311 329 320 340 413 421 448 GROUP: FEMALE 6 - 100 PPM C91451 406 419 447 454 471 474 496 485 482 C91452 581 C91453 490 498 527 524 559 561 446 361 348 C91454 350 373 337 C91455 385 401 410 429 445 450 452 460 454 C91456 443 351 C91457 473 505 506 534 553 578 C91459 517 553 565 563 586 600 614 580 586 C91461 578 541 493 404 C91462 491 508 510 518 531 531 524 528 513 C91463 433 438 436 430 C91464 293 C91465 465 480 355 269 239 C91466 539 571 622 632 607 473 C91467 395 419 423 445 444 454 491 500 511 C91471 458 462 446 441 425 418 310 C91472 546 551 584 603 631 656 641 C91473 408 420 446 460 485 493 513 524 529 1304 Appendix 3 Covance 6329-212 3M T-6316.1 Individual Body Weight Data (g) PAGE: 79 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 100 PPM C91474 458 473 494 453 371 315 C91475 554 598 609 610 630 671 678 C91476 451 387 C91477 463 478 416 339 C91478 518 534 564 539 C91479 518 539 556 557 569 582 607 630 638 C91480 389 400 420 432 438 452 462 456 462 C91481 317 C91482 463 463 416 371 309 284 C91483 409 427 438 440 446 434 C91485 382 391 397 415 418 441 464 473 474 C91486 470 446 359 357 263 C91487 530 563 604 608 636 669 690 708 726 C91488 462 479 488 476 502 496 521 521 537 C91489 535 550 583 C91490 437 477 459 1305 APPENDIX 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) Individual Test Material Consumption Data (mg/kg/day) 1306 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C90711 175 177 188 176 177 179 186 180 176 172 C90712 162 163 187 178 173 174 180 175 183 175 C90713 177 182 193 222 218 230 224 218 206 194 C90714 160 165 201 184 183 189 195 197 182 185 C90715 182 180 178 178 175 186 194 185 189 184 C90716 157 167 171 168 163 166 169 165 166 159 C90717 171 165 181 187 188 188 190 NOT TAKEN C90718 SPILLED 162 169 174 170 176 177 NOT TAKEN C90719 156 160 167 NOT TAKEN C90720 176 186 194 195 196 197 199 192 195 191 C90721 188 192 211 235 253 248 257 260 SPILLED 178 C90722 169 196 SPILLED 213 172 SPILLED 161 170 163 162 C90723 182 196 201 209 213 217 218 214 218 208 C90724 189 183 182 191 189 189 179 178 166 165 C90725 170 173 177 NOT TAKEN C90726 162 172 175 183 191 189 SPILLED SPILLED SPILLED 187 C90727 181 191 195 207 201 206 207 208 204 201 C90728 165 169 177 172 176 178 179 177 176 174 C90729 176 183 190 192 192 194 183 188 184 180 C90730 184 184 195 190 185 196 193 196 185 183 C90731 179 197 206 214 212 206 212 216 209 205 C90732 200 199 209 NOT TAKEN C90733 193 198 197 209 202 199 202 200 191 197 C90734 230 225 226 221 217 224 220 223 213 218 C90735 175 177 182 184 187 181 176 177 169 178 C90736 228 SPILLED 181 190 186 190 199 198 185 183 C90737 161 168 176 180 186 186 184 NOT TAKEN C90738 185 176 204 184 215 193 191 199 200 195 C90739 165 174 SPILLED 183 185 190 186 190 173 175 C90740 155 168 172 196 177 177 177 186 175 180 C90741 152 160 166 175 183 187 182 182 179 174 1307 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C90742 159 164 170 177 182 181 178 177 170 174 C90743 171 180 185 197 198 196 192 197 193 192 C90744 180 187 194 202 191 191 193 186 201 210 C90745 163 172 172 181 177 178 181 174 174 175 C90746 165 178 186 199 201 201 204 SPILLED 199 202 C90747 167 176 177 176 183 170 180 179 170 172 C90748 193 196 200 202 205 208 209 201 193 200 C90749 158 167 165 169 161 158 166 162 166 170 C90750 163 171 174 177 171 160 165 169 170 163 C90751 SPILLED 181 181 184 184 182 SPILLED SPILLED SPILLED 200 C90752 201 211 206 216 206 220 222 SPILLED 215 210 C90753 171 183 182 NOT TAKEN C90754 188 197 208 230 SPILLED SPILLED SPILLED SPILLED 214 228 C90755 187 193 194 195 200 204 209 210 213 213 C90756 146 152 160 165 162 165 166 NOT TAKEN C90757 181 178 184 192 189 187 189 189 186 187 C90758 171 177 183 202 200 224 SPILLED 226 215 205 C90759 163 174 183 190 189 190 191 185 176 173 C90760 185 199 SPILLED 194 210 194 SPILLED 193 196 180 C90761 171 172 165 176 174 174 177 175 174 176 C90762 177 188 182 183 182 178 201 169 179 165 C90763 188 186 195 193 202 197 202 195 199 194 C90764 172 186 187 197 192 197 SPILLED 205 SPILLED 182 C90765 197 200 207 204 200 206 211 205 208 202 C90766 162 167 190 178 176 180 180 178 164 177 C90767 154 159 169 166 176 177 179 174 199 178 C90768 169 182 184 188 184 204 214 176 176 171 C90769 175 182 181 183 178 180 183 NOT TAKEN C90770 180 171 172 189 188 SPILLED SPILLED 178 155 172 C90771 177 180 176 183 186 183 184 177 175 173 C90772 182 199 SPILLED 191 192 193 191 192 179 175 1308 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C90773 174 184 181 192 191 192 197 192 188 188 C90774 176 179 179 193 185 182 182 179 175 179 C90775 176 179 SPILLED NOT TAKEN C90776 193 185 196 203 199 206 212 215 205 200 C90777 177 188 193 206 205 211 215 218 209 202 C90778 167 168 171 176 176 176 180 173 167 167 C90779 181 192 197 193 189 188 186 183 181 178 C90780 166 177 182 189 193 195 201 203 203 198 GROUP: MALE 2 - 3 PPM C90781 162 169 169 172 171 174 172 SPILLED 182 182 C90782 SPILLED 169 182 189 SPILLED 198 234 186 205 191 C90783 145 148 157 164 167 166 158 164 160 159 C90784 161 167 173 175 174 176 168 170 170 171 C90785 163 167 172 177 177 184 187 191 186 191 C90786 177 175 168 179 179 176 176 175 161 163 C90787 162 167 173 181 187 195 199 198 197 197 C90788 217 180 188 188 185 181 181 180 178 177 C90789 176 186 197 NOT TAKEN C90790 186 190 198 197 204 203 209 210 204 206 C90791 145 158 157 168 177 173 174 173 175 170 C90792 165 175 181 197 199 193 187 191 192 183 C90793 161 169 181 182 180 177 182 180 178 171 C90794 173 194 195 198 214 202 201 204 205 201 C90795 168 177 176 182 191 183 192 197 194 193 C90796 179 185 206 199 198 194 210 214 221 219 C90797 156 159 165 168 172 174 182 180 173 175 C90798 188 195 201 137 182 176 195 175 197 178 C90799 168 171 174 174 169 169 177 176 170 165 C90800 174 185 SPILLED 185 185 192 190 194 184 185 1309 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 3 PPM C90801 C90802 C90803 C90804 C90805 C90806 C90807 C90808 C90809 C90810 C90811 C90812 C90813 C90814 C90815 C90816 C90817 C90818 C90819 C90820 C90821 C90822 C90823 C90824 C90825 C90826 C90827 C90828 C90829 C90830 C90831 169 174 182 190 198 196 200 205 203 203 147 160 156 164 160 162 160 161 152 154 185 199 195 200 205 202 190 192 185 181 190 204 209 212 211 218 222 212 213 210 195 198 193 202 205 208 200 199 195 197 149 152 150 164 168 165 162 161 159 160 172 178 183 187 183 175 176 177 173 181 174 183 189 193 202 198 199 200 199 198 171 173 173 180 SPILLED 178 188 177 176 189 173 190 192 194 197 196 198 191 191 188 172 172 175 186 205 198 203 198 195 202 165 172 174 177 173 177 181 181 183 172 150 152 156 157 162 162 154 NOT TAKEN 144 151 158 NOT TAKEN 167 177 183 185 189 193 198 196 194 195 177 178 177 192 188 189 196 192 198 200 161 161 164 NOT TAKEN 171 190 185 193 199 205 SPILLED 203 SPILLED 221 183 191 196 190 191 200 193 192 185 182 165 163 171 172 178 175 180 174 176 178 189 190 194 202 199 208 206 204 198 197 138 146 152 154 153 156 163 159 159 159 148 152 155 161 158 159 167 169 171 175 180 180 191 181 177 191 188 187 177 174 175 186 198 194 193 195 195 190 193 194 204 SPILLED SPILLED SPILLED SPILLED SPILLED SPILLED SPILLED SPILLED 245 160 172 179 182 185 185 188 183 184 179 178 195 203 215 209 219 212 221 223 227 191 203 201 203 202 199 206 203 203 195 175 186 192 193 201 191 187 191 188 186 169 180 178 183 185 186 195 203 201 193 1310 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 3 PPM C90832 177 191 198 208 202 199 207 196 190 188 C90833 186 202 218 NOT TAKEN C90834 179 192 195 NOT TAKEN C90835 174 179 175 182 186 183 208 189 195 182 C90836 168 162 161 179 183 181 183 181 178 179 C90837 163 163 168 175 173 179 174 172 168 164 C90838 193 202 203 210 214 212 215 208 213 211 C90839 186 193 198 208 213 217 214 219 214 206 C90840 167 177 183 188 195 193 197 197 196 192 GROUP: MALE 3 - 30 PPM C90841 160 164 C90842 138 154 C90843 167 177 C90844 179 186 C90845 SPILLED 174 C90846 149 151 C90847 159 165 C90848 159 168 C90849 170 175 C90850 154 156 C90851 161 178 C90852 172 197 C90853 SPILLED 149 C90854 184 195 C90855 210 197 C90856 173 182 C90857 170 174 C90858 151 156 C90859 178 188 164 170 184 189 188 185 184 180 132 145 146 142 148 141 149 142 173 189 180 191 SPILLED 183 187 189 187 SPILLED 190 SPILLED 188 SPILLED 200 192 177 179 183 185 183 183 179 176 148 NOT TAKEN 168 171 166 170 165 163 171 163 175 185 186 185 188 188 195 184 178 176 176 183 175 176 181 193 164 165 168 167 172 165 165 159 172 183 195 193 190 179 SPILLED 175 179 178 179 183 180 191 178 181 164 NOT TAKEN 194 194 SPILLED SPILLED SPILLED SPILLED SPILLED 195 194 187 195 185 182 185 182 183 174 183 177 178 189 178 179 184 168 182 181 184 188 178 182 182 152 159 154 159 165 163 148 152 199 195 191 190 196 193 192 192 1311 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90860 163 167 166 165 170 SPILLED 166 169 154 157 C90861 158 158 167 172 173 178 177 181 173 173 C90862 148 159 156 169 156 163 153 156 152 159 C90863 216 226 229 SPILLED 226 216 215 214 210 204 C90864 176 178 177 172 169 175 181 183 176 170 C90865 162 158 165 166 157 160 157 159 155 159 C90866 177 161 160 NOT TAKEN C90867 160 162 165 162 165 164 171 165 169 163 C90868 184 174 184 193 195 198 194 195 195 191 C90869 194 197 204 202 199 189 190 187 185 175 C90870 184 SPILLED SPILLED SPILLED 192 192 189 188 187 193 C90871 158 162 162 168 171 168 163 154 154 156 C90872 156 SPILLED 166 172 173 179 175 172 172 165 C90873 178 179 182 194 201 203 201 191 203 198 C90874 175 179 184 189 187 189 188 193 194 194 C90875 161 165 172 169 174 170 167 169 178 166 C90876 204 202 213 202 196 185 197 197 209 204 C90877 157 158 166 174 177 170 170 169 146 168 C90878 147 154 158 172 172 171 172 179 174 170 C90879 180 171 184 185 178 175 184 185 188 186 C90880 192 197 206 196 199 198 201 203 206 198 C90881 176 182 189 193 191 182 186 181 188 184 C90882 172 174 178 180 185 189 188 188 187 185 C90883 182 202 191 SPILLED 220 215 SPILLED 215 214 220 C90884 174 173 183 171 171 174 177 179 173 183 C90885 177 174 176 168 167 160 168 165 157 159 C90886 190 201 188 199 211 203 202 205 203 195 C90887 173 174 177 176 180 174 179 184 180 183 C90888 167 166 171 176 171 162 168 167 165 165 C90889 168 168 171 159 161 160 165 168 156 154 C90890 187 185 186 NOT TAKEN 1312 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90891 C90892 C90893 C90894 C90895 C90896 C90897 C90898 C90899 C90900 180 194 196 NOT TAKEN 130 123 127 132 141 181 181 183 181 185 182 177 169 174 181 191 200 190 171 192 194 SPILLED SPILLED SPILLED 181 181 187 191 195 204 168 169 168 169 175 151 156 157 156 157 166 164 171 174 173 139 146 149 148 148 185 188 187 179 174 178 185 175 172 167 172 180 184 199 187 181 SPILLED 169 SPILLED 209 197 204 199 195 186 170 182 179 169 171 154 168 167 163 153 169 175 184 176 173 GROUP: MALE 4 - 100 PPM C90901 154 151 147 155 158 161 155 150 144 151 C90902 165 159 152 162 161 158 152 155 145 150 C90903 154 150 149 155 157 159 153 148 147 139 C90904 169 162 166 169 162 160 164 159 156 156 C90905 156 SPILLED 160 168 168 162 161 165 162 164 C90906 161 153 157 168 173 167 167 164 171 166 C90907 155 148 153 158 162 159 152 150 149 153 C90908 174 169 176 180 179 177 172 171 167 171 C90909 179 188 188 191 183 181 193 198 185 188 C90910 162 155 161 164 169 173 174 175 166 169 C90911 175 167 167 167 173 170 166 164 172 171 C90912 162 160 167 173 178 175 178 169 177 170 C90913 169 175 171 174 172 155 89 140 187 185 C90914 188 184 185 188 189 183 187 182 182 182 C90915 175 174 186 191 198 193 183 190 179 183 C90916 142 147 150 149 152 147 142 150 146 145 C90917 174 174 183 180 185 179 179 181 172 159 C90918 174 179 179 179 182 180 184 188 177 168 1313 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90919 182 178 189 187 192 185 185 C90920 162 162 165 172 169 165 169 C90921 169 169 164 159 170 167 169 C90922 162 153 163 157 160 158 153 C90923 176 167 171 168 166 166 165 C90924 165 164 175 172 177 174 184 C90925 176 SPILLED 168 160 158 160 155 C90926 169 180 185 182 185 182 182 C90927 176 181 180 184 185 181 178 C90928 151 152 157 158 157 156 157 C90929 151 159 150 154 157 156 152 C90930 190 179 176 175 155 174 177 C90931 161 153 158 172 177 181 172 C90932 163 149 154 151 142 151 152 C90933 188 188 192 197 195 194 193 C90934 159 157 156 153 162 160 154 C90935 182 178 177 NOT TAKEN C90936 171 165 173 154 170 172 173 C90937 173 209 170 176 154 183 170 C90938 156 158 162 165 161 160 165 C90939 164 157 160 NOT TAKEN C90940 203 186 193 198 197 194 194 C90941 199 174 180 SPILLED 190 185 190 C90942 164 164 163 NOT TAKEN C90943 SPILLED 180 SPILLED SPILLED 202 SPILLED 188 C90944 139 139 144 142 138 140 143 C90945 157 157 156 156 155 151 154 C90946 168 157 SPILLED 165 172 170 164 C90947 166 161 163 164 163 165 162 C90948 206 173 171 173 176 176 175 C90949 199 166 156 149 162 140 131 187 187 179 168 171 166 170 165 170 159 147 152 171 165 172 184 173 181 160 151 154 184 177 173 174 169 162 154 149 149 159 159 159 179 179 180 169 168 162 154 150 149 193 182 184 154 172 166 175 168 168 160 SPILLED 169 169 165 160 199 194 184 187 176 180 177 167 190 151 150 144 156 151 142 163 150 160 156 158 159 177 171 163 147 129 104 1314 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90950 177 177 179 170 169 175 179 182 167 169 C90951 169 168 165 146 163 170 173 177 163 165 C90952 195 197 206 202 193 189 192 194 180 177 C90953 154 156 163 171 165 165 167 170 163 157 C90954 164 162 169 159 165 162 168 171 157 150 C90955 188 184 186 177 174 163 166 166 165 167 C90956 165 161 167 168 172 175 180 183 169 110 C90957 158 159 161 165 168 168 128 156 164 156 C90958 176 166 169 175 181 177 166 171 155 154 C90959 162 163 169 164 168 170 173 177 165 168 C90960 174 194 174 176 181 178 195 178 167 168 C90961 155 154 SPILLED NOT TAKEN C90962 189 188 185 190 190 189 185 190 178 178 C90963 176 173 181 184 188 182 186 184 177 176 C90964 206 208 210 205 209 203 201 197 193 192 C90965 165 166 166 163 159 157 135 152 147 143 C90966 181 187 187 NOT TAKEN C90967 148 152 153 166 174 175 166 167 161 160 C90968 147 150 150 164 165 168 167 166 157 154 C90969 177 179 177 181 182 185 181 196 189 202 C90970 155 151 156 169 173 157 SPILLED 160 157 155 GROUP: MALE 5 - 300 PPM C90971 131 110 107 111 110 120 116 NOT TAKEN C90972 129 121 125 120 112 108 112 NOT TAKEN C90973 167 162 157 157 164 161 151 NOT TAKEN C90974 158 147 142 145 138 138 138 NOT TAKEN C90975 176 174 178 168 170 174 179 NOT TAKEN C90976 163 144 137 137 130 127 130 NOT TAKEN C90977 162 144 147 135 SPILLED SPILLED SPILLED NOT TAKEN 1315 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 300 PPM C90978 C90979 C90980 C90981 C90982 C90983 C90984 C90985 C90986 C90987 C90988 C90989 C90990 C90991 C90992 C90993 C90994 C90995 C90996 C90997 C90998 C90999 C91000 C91001 C91002 C91003 C91004 C91005 C91006 C91007 C91008 159 142 174 172 157 130 167 158 165 158 212 173 161 149 154 163 169 163 173 162 143 157 158 171 139 152 183 190 145 156 SPILLED 154 138 169 165 158 146 154 153 163 148 170 166 157 150 147 155 157 EXCLUDE 161 153 143 148 145 164 133 139 SPILLED 159 138 157 172 SPILLED 131 169 159 158 127 156 136 156 138 155 161 161 147 142 158 157 156 153 141 146 144 136 166 132 134 146 160 132 153 167 145 127 166 151 151 117 NOT TAKEN 131 165 137 133 158 148 155 133 154 156 150 150 138 146 137 130 176 133 137 145 NOT TAKEN 126 155 SPILLED 142 133 169 155 145 109 134 SPILLED 139 SPILLED 156 153 143 134 160 153 152 146 139 153 137 138 174 128 127 SPILLED 128 156 SPILLED 134 127 163 151 156 114 128 149 139 SPILLED 150 154 142 143 152 157 140 148 143 161 135 128 184 131 132 145 125 160 SPILLED 133 137 164 149 148 113 132 147 147 SPILLED 155 153 149 144 145 154 149 150 142 163 139 124 174 128 NOT TAKEN 141 111 156 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1316 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 300 PPM C91009 179 C91010 161 C91011 170 C91012 143 C91013 191 C91014 149 C91015 166 C91016 148 C91017 159 C91018 180 C91019 178 C91020 161 C91021 151 C91022 169 C91023 162 C91024 135 C91025 C91026 157 C91027 138 C91028 163 C91029 151 C91030 164 C91031 133 C91032 170 C91033 159 C91034 158 C91035 177 C91036 152 C91037 172 C91038 196 C91039 184 176 150 135 137 153 146 138 134 163 162 159 157 133 134 131 131 187 185 168 169 160 146 SPILLED 138 158 159 148 151 141 139 135 134 143 137 142 141 171 168 159 162 167 159 155 155 150 SPILLED 143 145 159 157 161 157 165 158 165 162 156 155 161 156 132 125 NOT TAKEN 187 181 171 170 144 SPILLED 142 147 138 134 140 133 153 149 158 160 147 137 141 135 159 152 159 153 133 129 130 121 165 163 162 173 152 148 NOT TAKEN 148 152 157 157 161 152 152 161 129 141 134 132 164 156 NOT TAKEN 153 150 154 149 179 172 180 183 145 142 NOT TAKEN 134 135 NOT TAKEN 153 157 NOT TAKEN 121 126 NOT TAKEN 166 176 NOT TAKEN 138 140 NOT TAKEN 150 157 NOT TAKEN 128 136 NOT TAKEN 136 137 NOT TAKEN 154 154 NOT TAKEN 155 145 NOT TAKEN 145 140 NOT TAKEN 154 SPILLED NOT TAKEN 162 160 NOT TAKEN 163 156 NOT TAKEN 171 165 NOT TAKEN 145 147 NOT TAKEN 133 125 NOT TAKEN 154 154 NOT TAKEN 140 NOT TAKEN 162 164 NOT TAKEN 122 105 NOT TAKEN 169 153 NOT TAKEN 155 147 NOT TAKEN 160 155 NOT TAKEN 129 126 NOT TAKEN 149 142 NOT TAKEN 168 153 NOT TAKEN 1317 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 300 PPM C91040 165 157 160 159 162 157 159 NOT TAKEN GROUP: MALE 6 - 100 PPM C91041 182 175 175 178 180 182 180 184 186 184 C91042 173 167 168 165 164 164 157 157 161 168 C91043 168 161 164 171 170 176 173 181 173 176 C91044 168 158 166 177 178 176 173 175 165 177 C91045 176 177 183 194 189 193 183 177 177 175 C91046 174 170 175 173 182 129 NOT TAKEN C91047 161 162 161 163 158 161 158 95 137 157 C91048 160 164 161 161 164 177 170 176 179 180 C91049 182 185 187 191 191 194 195 201 200 196 C91050 194 189 201 209 198 202 191 200 172 179 C91051 175 171 181 183 184 182 184 190 198 200 C91052 191 221 200 212 SPILLED 209 207 211 226 206 C91053 SPILLED 187 168 163 161 161 178 SPILLED 158 155 C91054 179 183 184 185 185 179 179 183 172 161 C91055 184 181 185 194 190 190 192 196 186 188 C91056 163 166 163 170 165 162 158 116 166 173 C91057 167 169 166 172 171 171 168 175 169 165 C91058 176 171 169 176 172 174 161 162 154 144 C91059 163 163 169 177 182 178 175 179 173 175 C91060 195 SPILLED 156 164 170 168 168 170 161 165 C91061 163 165 168 170 171 168 171 176 175 175 C91062 169 199 179 179 180 179 180 180 174 165 C91063 153 158 152 146 156 150 142 149 135 131 C91064 157 152 159 168 178 172 170 171 163 164 C91065 169 166 168 170 174 175 170 168 162 158 C91066 170 171 174 171 176 169 175 184 175 175 C91067 170 169 170 170 170 174 174 178 173 169 1318 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 100 PPM C91068 144 132 121 112 106 91 94 89 33 39 C91069 176 172 172 167 175 180 178 187 180 176 C91070 163 157 162 166 165 162 161 162 160 160 C91071 182 186 189 192 194 194 190 196 193 190 C91072 154 155 161 158 156 156 156 157 150 148 C91073 178 181 185 178 183 182 178 184 174 183 C91074 162 159 162 165 160 157 158 155 152 149 C91075 171 179 187 181 196 181 181 181 171 174 C91076 169 167 169 163 160 124 128 168 166 165 C91077 158 158 164 165 169 169 170 167 168 165 C91078 157 154 157 159 155 152 147 148 141 142 C91079 179 177 179 184 185 185 185 181 170 169 C91080 173 166 168 171 175 169 171 174 172 170 GROUP: MALE 7 - 300 PPM C91081 SPILLED 150 145 189 146 144 142 NOT TAKEN C91082 143 134 130 126 127 121 124 NOT TAKEN C91083 151 139 135 134 135 142 138 NOT TAKEN C91084 141 134 131 130 135 136 139 NOT TAKEN C91085 SPILLED 154 143 146 129 120 108 NOT TAKEN C91086 155 146 153 150 148 143 140 NOT TAKEN C91087 158 151 139 134 132 123 122 NOT TAKEN C91088 153 150 150 146 137 131 130 NOT TAKEN C91089 180 170 165 167 170 174 164 NOT TAKEN C91090 151 157 155 150 155 151 160 NOT TAKEN C91091 155 149 144 152 150 142 138 NOT TAKEN C91092 169 156 152 156 150 157 146 NOT TAKEN C91093 154 144 145 111 150 157 153 NOT TAKEN C91094 152 141 133 126 128 133 129 NOT TAKEN C91095 164 148 SPILLED 154 155 156 142 NOT TAKEN 1319 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 7 - 300 PPM C91096 C91097 C91098 C91099 C91100 C91101 C91102 C91103 C91104 C91105 C91106 C91107 C91108 C91109 C91110 C91111 C91112 C91113 C91114 C91115 C91116 C91117 C91118 C91119 C91120 136 134 129 128 134 130 126 NOT TAKEN 169 153 147 142 144 140 139 NOT TAKEN 166 158 156 154 154 148 149 NOT TAKEN 153 129 126 143 114 NOT TAKEN 162 153 143 143 139 132 135 NOT TAKEN 169 160 159 166 159 166 175 NOT TAKEN 156 191 153 144 154 156 146 NOT TAKEN 152 141 144 144 141 141 134 NOT TAKEN 132 135 128 122 127 124 121 NOT TAKEN 159 159 136 132 135 138 139 NOT TAKEN 153 146 142 126 149 142 152 NOT TAKEN 142 136 133 135 135 135 133 NOT TAKEN 165 159 156 154 149 152 151 NOT TAKEN 154 153 142 139 140 148 135 NOT TAKEN 166 155 160 151 155 155 144 NOT TAKEN 147 142 138 135 141 149 150 NOT TAKEN 176 170 168 166 168 165 165 NOT TAKEN 158 140 138 127 123 115 127 NOT TAKEN 187 170 169 156 163 162 155 NOT TAKEN 176 172 173 167 158 164 160 NOT TAKEN 155 144 141 133 142 138 165 NOT TAKEN 189 SPILLED SPILLED 193 SPILLED 178 SPILLED NOT TAKEN 158 148 149 146 149 162 139 NOT TAKEN 176 172 171 168 175 175 172 NOT TAKEN 179 164 166 163 168 132 27 NOT TAKEN GROUP: FEMALE 1 - 0 PPM C91121 118 SPILLED 143 143 139 141 150 144 137 145 C91122 SPILLED 136 SPILLED 162 146 148 169 157 SPILLED 134 C91123 108 122 121 136 127 126 129 114 114 119 1320 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C91124 C91125 C91126 C91127 C91128 C91129 C91130 C91131 C91132 C91133 C91134 C91135 C91136 C91137 C91138 C91139 C91140 C91141 C91142 C91143 C91144 C91145 C91146 C91147 C91148 C91149 C91150 C91151 C91152 C91153 C91154 128 102 189 117 134 130 131 SPILLED 183 127 134 115 136 134 106 174 140 127 129 104 122 130 116 125 120 104 115 131 120 134 134 129 109 SPILLED 127 160 147 128 123 SPILLED 130 137 117 143 115 102 240 156 115 145 112 115 140 125 118 118 123 120 125 134 146 146 137 112 SPILLED 137 150 156 136 SPILLED SPILLED SPILLED 142 SPILLED 140 109 SPILLED SPILLED 165 130 150 131 126 135 126 123 138 117 141 128 124 138 163 143 114 142 152 157 167 145 SPILLED SPILLED 138 140 NOT TAKEN 149 105 110 SPILLED SPILLED 134 159 NOT TAKEN 124 145 133 135 139 SPILLED 131 146 130 161 150 142 117 SPILLED 134 175 156 165 SPILLED SPILLED 148 137 143 111 107 193 186 137 158 115 145 138 132 129 159 119 168 146 148 149 139 124 137 134 155 152 SPILLED SPILLED SPILLED 142 142 152 115 111 146 176 141 151 117 138 135 160 130 SPILLED 124 149 137 142 133 139 113 SPILLED 136 159 156 147 SPILLED SPILLED 156 142 156 104 107 SPILLED 162 SPILLED 150 118 129 133 139 160 SPILLED 125 168 143 138 147 137 120 NOT TAKEN 131 153 150 146 158 SPILLED 140 145 142 106 107 SPILLED NOT TAKEN 136 140 114 NOT TAKEN 130 129 132 SPILLED 119 154 140 129 148 122 121 125 143 145 148 SPILLED SPILLED 125 132 149 100 113 136 133 136 111 131 125 131 113 120 149 130 131 138 132 113 128 143 156 140 SPILLED SPILLED 131 144 144 108 108 SPILLED 131 147 112 136 147 SPILLED SPILLED 122 143 129 125 127 1321 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C91155 C91156 C91157 C91158 C91159 C91160 C91161 C91162 C91163 C91164 C91165 C91166 C91167 C91168 C91169 C91170 C91171 C91172 C91173 C91174 C91175 C91176 C91177 C91178 C91179 C91180 C91181 C91182 C91183 C91184 C91185 SPILLED 119 124 121 112 122 108 124 130 135 147 139 SPILLED SPILLED 115 SPILLED 121 202 125 115 157 126 SPILLED 134 144 122 151 124 233 127 136 SPILLED 132 114 127 118 121 121 127 132 136 134 140 SPILLED SPILLED 130 170 118 SPILLED 128 121 158 147 SPILLED 133 134 133 167 139 SPILLED 139 137 SPILLED 134 127 130 108 131 118 134 141 132 142 147 146 SPILLED 136 179 130 SPILLED 132 116 164 146 115 140 160 136 163 144 SPILLED 156 140 SPILLED 134 125 126 116 147 137 127 136 134 NOT TAKEN 148 132 152 NOT TAKEN SPILLED NOT TAKEN 158 131 116 175 155 SPILLED 139 154 139 168 137 SPILLED 173 148 SPILLED 132 131 129 108 SPILLED 140 136 129 143 146 138 SPILLED SPILLED 150 122 113 163 153 SPILLED 143 152 142 165 151 SPILLED 162 138 SPILLED 151 127 121 116 SPILLED 143 142 139 120 145 126 SPILLED SPILLED 137 125 112 185 151 108 142 149 143 155 144 SPILLED 155 151 SPILLED 131 135 120 106 SPILLED 148 133 125 132 134 132 170 SPILLED SPILLED 136 118 176 163 112 144 160 145 151 146 SPILLED 148 148 SPILLED 94 123 116 SPILLED 132 131 NOT TAKEN NOT TAKEN 144 154 129 157 SPILLED 133 123 116 172 143 110 140 145 156 142 142 SPILLED 150 145 SPILLED 123 121 121 127 125 149 123 146 123 143 147 129 115 108 174 162 109 137 136 128 129 141 SPILLED 147 138 190 108 121 114 105 170 148 133 136 126 SPILLED SPILLED 139 118 119 185 SPILLED 108 136 139 139 133 140 SPILLED 146 143 1322 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C91186 121 C91187 140 C91188 103 C91189 88 C91190 116 127 136 139 138 138 141 137 134 130 143 146 156 157 148 145 144 151 147 107 SPILLED 144 130 132 136 119 127 128 91 99 109 106 104 106 106 108 104 110 133 SPILLED SPILLED SPILLED SPILLED SPILLED SPILLED 217 GROUP: FEMALE 2 - 3 PPM C91191 119 126 125 131 145 134 157 148 151 145 C91192 109 114 114 138 119 137 125 134 125 124 C91193 SPILLED SPILLED 246 SPILLED SPILLED SPILLED SPILLED SPILLED SPILLED 156 C91194 111 116 127 129 135 147 146 138 143 145 C91195 138 149 151 154 146 156 164 150 136 141 C91196 107 118 125 115 111 109 115 104 97 97 C91197 127 136 137 160 169 SPILLED SPILLED 146 154 190 C91198 125 123 138 NOT TAKEN C91199 139 148 141 SPILLED 167 SPILLED SPILLED SPILLED SPILLED SPILLED C91200 125 123 141 175 137 128 107 126 130 142 C91201 104 SPILLED 121 146 129 166 132 SPILLED 113 127 C91202 127 142 143 153 139 133 129 125 124 124 C91203 129 138 144 150 148 138 129 130 117 114 C91204 121 123 128 132 136 149 137 169 130 148 C91205 111 117 125 NOT TAKEN C91206 SPILLED 120 125 128 135 135 136 136 130 138 C91207 123 128 123 147 146 146 148 145 142 136 C91208 117 124 124 SPILLED 134 143 134 SPILLED 127 140 C91209 112 113 120 116 116 125 121 124 118 124 C91210 179 151 159 SPILLED SPILLED SPILLED 144 SPILLED SPILLED SPILLED C91211 125 136 131 143 SPILLED 195 138 133 133 134 C91212 107 111 119 125 SPILLED 122 122 119 107 112 C91213 116 129 131 134 127 127 132 131 126 125 1323 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91214 C91215 C91216 C91217 C91218 C91219 C91220 C91221 C91222 C91223 C91224 C91225 C91226 C91227 C91228 C91229 C91230 C91231 C91232 C91233 C91234 C91235 C91236 C91237 C91238 C91239 C91240 C91241 C91242 C91243 C91244 138 111 127 104 112 135 117 125 139 96 141 121 105 SPILLED 129 122 130 129 112 113 137 135 121 143 110 118 144 123 SPILLED 134 105 142 113 SPILLED 111 150 130 130 135 147 96 149 118 112 116 130 134 138 140 119 114 159 SPILLED 121 149 113 117 127 127 174 155 113 144 114 SPILLED 117 SPILLED 127 134 136 148 108 117 141 120 SPILLED 126 128 141 142 111 112 163 193 132 135 110 136 143 125 SPILLED 163 118 137 110 SPILLED 130 SPILLED SPILLED 129 137 NOT TAKEN 114 SPILLED 106 111 SPILLED 160 124 NOT TAKEN SPILLED 125 129 164 SPILLED 128 NOT TAKEN 114 135 139 136 209 SPILLED 118 141 106 SPILLED 127 137 153 129 137 117 132 118 124 SPILLED 148 122 138 106 125 167 SPILLED 130 112 135 141 135 SPILLED 158 115 148 115 158 127 SPILLED SPILLED 134 161 112 125 126 131 122 142 118 155 110 130 168 SPILLED 161 117 140 141 SPILLED SPILLED SPILLED 121 142 109 SPILLED 138 141 SPILLED 119 SPILLED 110 134 133 134 137 139 110 133 113 125 152 SPILLED SPILLED 118 140 147 135 SPILLED 142 127 143 116 SPILLED 132 179 185 125 150 115 SPILLED 100 139 124 145 117 136 113 133 154 SPILLED 128 106 134 141 203 180 SPILLED 122 135 111 SPILLED 126 170 SPILLED 117 143 104 122 108 136 115 136 108 130 109 117 162 174 109 108 124 140 126 SPILLED 161 104 131 104 SPILLED 126 SPILLED SPILLED 118 132 106 124 125 131 116 133 103 132 104 112 150 SPILLED 111 107 128 130 SPILLED SPILLED 187 111 1324 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91245 C91246 C91247 C91248 C91249 C91250 144 154 166 193 137 163 SPILLED 157 173 SPILLED 167 153 SPILLED SPILLED SPILLED SPILLED SPILLED SPILLED SPILLED SPILLED 134 146 SPILLED 146 153 166 174 197 SPILLED SPILLED 127 133 130 137 134 131 141 136 120 129 129 SPILLED 137 190 150 SPILLED 153 159 141 146 113 113 SPILLED 111 115 SPILLED 110 175 105 155 GROUP: FEMALE 3 - 30 PPM C91251 C91252 C91253 C91254 C91255 C91256 C91257 C91258 C91259 C91260 C91261 C91262 C91263 C91264 C91265 C91266 C91267 C91268 C91269 C91270 C91271 C91272 109 109 118 NOT TAKEN 122 128 132 135 142 115 125 112 129 127 118 121 120 124 132 111 116 117 140 124 134 115 SPILLED 136 140 105 112 132 SPILLED 109 121 135 135 123 135 158 SPILLED 124 133 127 133 137 130 131 138 106 105 SPILLED 127 120 SPILLED 105 101 112 100 125 123 131 131 127 112 119 SPILLED 126 125 127 124 119 152 190 133 140 145 158 158 102 103 105 109 106 117 SPILLED 111 121 118 128 138 131 142 148 123 117 123 123 125 136 SPILLED 138 SPILLED 207 140 145 129 NOT TAKEN 138 134 155 149 139 131 114 133 118 122 131 126 131 115 121 140 127 134 131 129 130 126 130 125 112 116 105 115 100 SPILLED 134 123 128 128 125 125 126 137 130 130 140 132 113 128 137 125 126 146 136 106 99 96 118 96 89 128 119 129 132 123 124 118 123 121 112 127 133 127 117 SPILLED 171 147 144 147 137 109 102 108 100 94 112 105 105 106 105 133 138 SPILLED 143 136 127 122 127 121 120 180 SPILLED SPILLED 144 SPILLED 1325 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 20 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 30 PPM C91273 C91274 C91275 C91276 C91277 C91278 C91279 C91280 C91281 C91282 C91283 C91284 C91285 C91286 C91287 C91288 C91289 C91290 C91291 C91292 C91293 C91294 C91295 C91296 C91297 C91298 C91299 C91300 C91301 C91302 C91303 SPILLED 110 125 103 107 125 158 126 113 134 122 131 113 100 157 121 SPILLED 122 131 119 136 127 118 101 173 131 SPILLED 165 115 SPILLED 127 146 117 138 106 111 SPILLED 184 130 130 135 126 138 112 99 106 122 123 119 122 126 176 130 149 SPILLED SPILLED 126 SPILLED SPILLED 112 SPILLED 135 SPILLED 112 133 114 115 SPILLED SPILLED 134 SPILLED 149 133 146 108 101 132 122 SPILLED 124 124 133 151 129 179 SPILLED SPILLED 123 112 186 123 SPILLED 138 178 SPILLED 139 115 106 141 162 132 SPILLED 155 130 149 NOT TAKEN 115 NOT TAKEN 119 124 133 NOT TAKEN 146 SPILLED 136 SPILLED 121 133 136 115 SPILLED 120 SPILLED 144 150 SPILLED 155 110 122 144 155 132 SPILLED 166 133 146 115 124 127 121 133 SPILLED 133 154 114 148 139 112 SPILLED 121 SPILLED 135 SPILLED SPILLED 139 114 124 SPILLED 168 126 SPILLED 168 143 144 SPILLED 114 122 121 127 SPILLED 123 SPILLED 119 151 131 120 198 125 SPILLED 134 SPILLED 118 143 121 118 133 164 130 SPILLED 162 131 129 138 109 117 134 125 SPILLED 124 133 113 158 124 110 169 120 138 134 147 SPILLED SPILLED 117 116 SPILLED 151 128 SPILLED 153 122 SPILLED 113 110 125 129 134 SPILLED SPILLED 217 125 134 128 110 228 129 SPILLED 127 SPILLED SPILLED SPILLED 115 107 115 150 134 SPILLED 158 131 135 115 108 109 126 122 124 120 140 SPILLED SPILLED 126 101 SPILLED 126 115 121 145 150 155 112 111 134 158 126 SPILLED 151 130 128 111 103 113 112 122 SPILLED 126 130 114 189 122 110 SPILLED 132 SPILLED 136 1326 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 21 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 30 PPM C91304 147 147 144 149 160 SPILLED 156 168 145 150 C91305 135 138 137 159 166 154 156 163 150 155 C91306 SPILLED SPILLED 148 SPILLED 130 SPILLED SPILLED SPILLED 121 120 C91307 129 137 128 145 136 142 124 163 123 119 C91308 126 133 142 150 148 162 162 170 188 SPILLED C91309 125 131 138 139 149 148 155 145 141 143 C91310 119 123 118 129 140 128 132 130 137 124 GROUP: FEMALE 4 - 100 PPM C91311 C91312 C91313 C91314 C91315 C91316 C91317 C91318 C91319 C91320 C91321 C91322 C91323 C91324 C91325 C91326 C91327 C91328 C91329 C91330 C91331 101 100 124 113 115 SPILLED 119 138 123 131 120 120 119 116 107 104 112 129 116 111 127 121 103 146 125 SPILLED SPILLED 122 152 125 SPILLED 133 127 113 116 103 93 109 125 118 110 119 SPILLED 97 SPILLED 125 119 132 124 146 117 SPILLED 133 SPILLED SPILLED 114 108 112 111 114 119 116 136 125 105 132 127 112 NOT TAKEN NOT TAKEN SPILLED 137 140 189 141 118 124 111 115 114 124 133 118 132 SPILLED 101 SPILLED SPILLED 112 112 131 127 143 132 SPILLED 117 103 109 134 116 116 107 135 143 98 SPILLED SPILLED 118 108 133 115 SPILLED 138 137 118 106 106 112 110 116 115 132 108 99 122 SPILLED 120 107 123 112 134 126 SPILLED 119 106 115 113 110 114 107 129 117 104 150 SPILLED 122 109 122 110 SPILLED 136 125 121 109 110 118 114 116 109 133 SPILLED 105 131 SPILLED 122 103 120 106 129 133 115 114 108 102 111 112 110 109 124 105 100 SPILLED SPILLED 124 94 120 110 125 123 114 107 99 99 112 109 108 108 132 1327 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 22 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91332 C91333 C91334 C91335 C91336 C91337 C91338 C91339 C91340 C91341 C91342 C91343 C91344 C91345 C91346 C91347 C91348 C91349 C91350 C91351 C91352 C91353 C91354 C91355 C91356 C91357 C91358 C91359 C91360 C91361 C91362 SPILLED 109 123 112 SPILLED 122 154 118 137 124 129 126 117 SPILLED SPILLED SPILLED 123 SPILLED SPILLED 121 133 120 137 123 137 98 87 137 119 SPILLED 153 124 117 124 114 SPILLED 115 SPILLED 113 147 117 116 125 113 111 141 SPILLED 118 125 SPILLED 130 126 122 SPILLED 139 137 93 132 SPILLED 129 126 195 136 SPILLED 125 118 SPILLED 115 139 123 150 128 111 112 113 110 131 SPILLED 120 121 SPILLED 121 134 113 SPILLED 123 140 99 130 131 SPILLED 120 189 130 NOT TAKEN 121 111 SPILLED 132 135 128 SPILLED 130 114 119 118 NOT TAKEN 139 SPILLED 122 119 NOT TAKEN 127 135 121 SPILLED 137 168 97 135 128 113 123 SPILLED 131 117 109 SPILLED 124 104 115 156 124 124 121 114 134 117 112 127 125 131 121 SPILLED 128 SPILLED 95 133 122 103 118 SPILLED 119 121 118 SPILLED 127 110 122 147 124 115 127 107 131 117 113 117 143 141 110 SPILLED 122 135 92 123 124 110 119 SPILLED 113 117 113 SPILLED 119 111 110 136 119 123 118 115 135 114 111 113 132 133 120 99 123 134 93 124 137 105 111 150 144 116 115 SPILLED 127 102 123 134 121 123 115 110 133 118 100 122 132 125 112 106 131 SPILLED 93 128 SPILLED 108 97 SPILLED 124 106 116 275 116 87 109 126 116 125 117 112 126 112 99 134 135 121 112 97 136 124 90 132 SPILLED 104 114 113 105 110 112 171 122 99 109 122 116 115 112 111 129 112 107 113 128 119 111 99 141 118 86 128 SPILLED 110 116 SPILLED 1328 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 23 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91363 136 140 143 149 127 138 132 139 125 121 C91364 117 125 115 118 111 108 96 102 96 97 C91365 SPILLED 140 SPILLED SPILLED SPILLED 114 109 133 SPILLED SPILLED C91366 124 110 122 127 118 129 122 128 118 103 C91367 152 146 143 130 135 142 122 142 117 122 C91368 135 160 SPILLED 161 143 119 118 114 100 102 C91369 114 113 113 114 125 122 116 116 119 122 C91370 126 130 119 119 119 115 109 115 115 110 C91371 110 117 119 123 131 125 SPILLED SPILLED SPILLED 180 C91372 147 122 152 136 145 119 119 SPILLED 111 117 C91373 120 117 107 116 112 111 109 109 110 102 C91374 SPILLED SPILLED 131 SPILLED 130 125 109 115 116 106 C91375 127 121 120 132 124 119 111 117 120 112 C91376 116 111 110 109 115 112 108 114 104 103 C91377 115 117 121 131 125 119 122 124 119 115 C91378 117 116 113 118 123 119 118 112 103 108 C91379 113 166 121 130 134 127 115 110 118 120 C91380 135 133 139 135 135 129 129 133 129 140 GROUP: FEMALE 5 - 300 PPM C91381 C91382 C91383 C91384 C91385 C91386 C91387 C91388 C91389 C91390 SPILLED 91 103 169 SPILLED 109 98 118 104 107 96 95 98 107 109 SPILLED 81 111 74 97 95 75 99 94 96 SPILLED 75 105 75 103 107 86 102 114 108 SPILLED 77 108 82 107 101 81 99 SPILLED 107 SPILLED 101 102 76 103 101 86 101 106 145 SPILLED 79 98 81 110 97 78 88 SPILLED 120 SPILLED 79 103 87 108 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1329 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 24 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 300 PPM C91391 C91392 C91393 C91394 C91395 C91396 C91397 C91398 C91399 C91400 C91401 C91402 C91403 C91404 C91405 C91406 C91407 C91408 C91409 C91410 C91411 C91412 C91413 C91414 C91415 C91416 C91417 C91418 C91419 C91420 C91421 109 SPILLED 86 110 124 SPILLED 117 114 117 126 106 92 SPILLED 94 106 109 111 118 SPILLED SPILLED 120 115 SPILLED 121 107 111 122 107 SPILLED 112 91 102 111 143 106 118 SPILLED 92 94 101 113 125 84 155 88 98 99 93 116 84 118 115 97 SPILLED 114 90 114 SPILLED 104 SPILLED 135 90 97 SPILLED SPILLED 96 168 99 94 103 93 124 93 84 141 90 94 91 99 109 82 90 107 99 SPILLED 104 95 100 109 106 100 103 83 100 91 73 94 101 111 99 102 91 133 90 88 SPILLED 95 98 SPILLED 94 SPILLED 76 84 105 86 SPILLED 103 77 98 109 100 SPILLED 103 79 94 103 66 89 SPILLED 94 101 98 90 131 85 83 SPILLED 92 103 92 97 116 87 83 102 95 107 116 88 103 94 103 99 94 87 92 96 66 88 98 97 102 100 93 132 85 85 SPILLED 97 106 92 SPILLED 106 80 81 105 98 SPILLED 113 85 105 91 94 98 95 84 94 101 72 88 98 96 97 95 97 125 84 86 SPILLED 93 105 93 99 108 83 85 98 87 94 103 86 99 93 93 102 96 87 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1330 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 25 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 300 PPM C91422 C91423 C91424 C91425 C91426 C91427 C91428 C91429 C91430 C91431 C91432 C91433 C91434 C91435 C91436 C91437 C91438 C91439 C91440 C91441 C91442 C91443 C91444 C91445 C91446 C91447 C91448 C91449 C91450 SPILLED 102 103 SPILLED 107 125 118 131 104 107 95 167 109 115 84 106 111 133 SPILLED 112 113 110 113 SPILLED 107 121 110 117 102 88 111 100 94 137 SPILLED 108 115 101 91 105 153 102 112 92 105 105 113 116 99 100 95 98 100 96 102 SPILLED 130 95 88 113 93 87 83 114 113 102 100 87 92 SPILLED 95 112 88 101 100 103 105 88 101 102 100 SPILLED 89 97 SPILLED SPILLED 95 79 NOT TAKEN 90 80 63 NOT TAKEN 108 105 NOT TAKEN SPILLED 104 SPILLED 96 114 85 106 103 SPILLED 105 96 100 NOT TAKEN 102 86 90 92 NOT TAKEN SPILLED 87 73 91 84 80 109 111 SPILLED 106 SPILLED 93 111 78 105 104 100 98 96 96 100 87 97 98 179 70 77 94 81 100 103 103 SPILLED 98 85 86 110 93 105 100 100 98 93 102 98 SPILLED 95 93 SPILLED 76 79 91 85 81 107 98 SPILLED 103 95 87 113 91 111 100 88 106 94 93 94 89 100 126 SPILLED 72 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1331 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 26 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 100 PPM C91451 103 105 103 113 120 113 108 141 103 110 C91452 116 116 116 123 124 115 125 133 120 129 C91453 124 121 118 128 126 123 122 132 119 121 C91454 122 117 113 115 112 103 108 105 96 106 C91455 124 155 146 121 119 116 115 130 117 117 C91456 124 107 104 110 105 102 99 103 99 106 C91457 126 128 116 125 127 130 122 132 125 121 C91458 107 103 117 105 114 106 102 111 102 103 C91459 135 152 126 138 139 SPILLED 112 SPILLED 125 SPILLED C91460 99 106 104 103 101 96 85 90 85 102 C91461 108 116 119 121 122 121 110 123 112 111 C91462 124 122 131 131 132 131 133 136 129 127 C91463 SPILLED 123 112 111 115 102 110 103 101 99 C91464 107 107 111 114 116 109 105 108 101 104 C91465 120 SPILLED 126 116 124 130 108 128 105 108 C91466 125 119 135 142 127 109 123 122 125 114 C91467 123 145 108 125 115 135 120 SPILLED 108 113 C91468 103 99 105 101 103 105 90 114 89 113 C91469 108 106 114 107 108 106 105 110 104 105 C91470 140 150 146 140 148 150 127 157 139 145 C91471 109 105 110 111 118 116 110 111 113 114 C91472 130 120 138 149 157 128 125 SPILLED SPILLED 136 C91473 111 103 107 107 111 106 118 SPILLED 106 129 C91474 145 150 140 150 122 144 136 163 145 SPILLED C91475 167 152 151 SPILLED 139 153 188 SPILLED 132 SPILLED C91476 SPILLED 121 116 124 123 120 120 120 116 120 C91477 103 103 106 SPILLED 112 107 117 114 110 120 C91478 SPILLED 159 151 135 130 123 123 SPILLED 136 128 C91479 160 160 142 144 142 134 132 147 138 124 C91480 119 115 118 113 109 108 108 106 105 110 C91481 112 111 116 114 112 111 104 103 105 113 1332 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 100 PPM C91482 115 118 127 130 125 122 119 120 119 115 C91483 149 131 158 SPILLED 149 114 131 SPILLED 116 106 C91484 114 113 115 114 118 115 104 116 110 105 C91485 112 115 115 113 112 114 104 117 105 106 C91486 118 114 118 122 116 110 111 108 110 106 C91487 133 115 111 110 111 104 104 113 104 103 C91488 136 133 SPILLED 155 126 122 123 123 120 C91489 102 105 114 121 111 110 108 114 118 109 C91490 120 124 121 SPILLED 124 115 116 118 123 112 GROUP: FEMALE 7 - 300 PPM C91491 C91492 C91493 C91494 C91495 C91496 C91497 C91498 C91499 C91500 C91501 C91502 C91503 C91504 C91505 C91506 C91507 C91508 C91509 109 SPILLED SPILLED 106 115 113 115 99 107 115 105 125 134 SPILLED 108 118 123 SPILLED 115 110 166 103 94 101 100 107 121 115 106 86 110 129 SPILLED 97 112 114 145 114 99 103 SPILLED 87 92 90 102 87 89 94 96 111 110 SPILLED 96 104 106 116 94 103 SPILLED 93 91 92 91 103 95 86 92 87 108 112 SPILLED 93 101 153 119 SPILLED 96 106 91 87 94 93 103 92 80 82 90 105 114 SPILLED 96 107 106 SPILLED SPILLED 93 101 85 83 86 86 101 118 78 83 SPILLED 100 104 164 SPILLED 103 103 SPILLED 95 90 97 89 89 97 90 103 SPILLED 80 80 84 105 115 121 91 102 98 SPILLED 89 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1333 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 28 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 7 - 300 PPM C91510 115 114 105 108 100 99 106 NOT TAKEN C91511 114 99 SPILLED 98 101 101 100 NOT TAKEN C91512 102 104 94 91 93 90 90 NOT TAKEN C91513 109 111 95 95 92 88 84 NOT TAKEN C91514 127 120 116 111 109 105 108 NOT TAKEN C91515 SPILLED SPILLED 109 94 94 108 99 NOT TAKEN C91516 109 97 91 98 93 96 90 NOT TAKEN C91517 98 SPILLED 81 81 88 83 83 NOT TAKEN C91518 113 114 94 100 100 98 107 NOT TAKEN C91519 105 SPILLED SPILLED SPILLED SPILLED SPILLED 171 NOT TAKEN C91520 105 96 95 98 97 95 104 NOT TAKEN C91521 113 102 97 88 101 95 84 NOT TAKEN C91522 150 133 108 104 124 97 94 NOT TAKEN C91523 111 137 139 101 112 105 105 NOT TAKEN C91524 SPILLED 107 102 98 113 105 111 NOT TAKEN C91525 169 108 102 100 98 97 97 NOT TAKEN C91526 117 105 102 104 101 100 102 NOT TAKEN C91527 127 SPILLED 111 104 SPILLED 112 109 NOT TAKEN C91528 101 85 84 81 74 84 75 NOT TAKEN C91529 SPILLED 131 132 123 107 111 104 NOT TAKEN C91530 110 101 91 100 89 92 94 NOT TAKEN 1334 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 29 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C90711 178 175 167 178 178 174 191 181 184 188 C90712 184 172 125 179 SPILLED SPILLED 188 186 177 164 C90713 200 198 210 203 211 207 209 223 217 211 C90714 185 186 188 187 179 176 191 183 183 180 C90715 172 172 182 179 193 187 191 182 194 190 C90716 158 155 157 SPILLED 159 163 162 161 160 162 C90720 187 182 179 182 187 182 192 189 192 193 C90721 189 199 258 267 257 SPILLED 246 247 245 243 C90722 163 161 163 164 167 169 169 167 181 178 C90723 SPILLED SPILLED SPILLED 195 199 196 209 202 203 215 C90724 174 164 168 169 177 173 173 173 172 175 C90726 203 SPILLED SPILLED 186 190 190 186 185 175 181 C90727 204 189 192 195 195 189 194 191 192 193 C90728 173 174 183 183 182 183 187 189 187 191 C90729 174 171 178 182 190 189 185 181 180 188 C90730 184 182 188 188 193 191 195 193 202 191 C90731 195 203 204 NOT TAKEN C90733 125 133 192 193 193 198 205 214 197 194 C90734 212 SPILLED 211 214 220 223 226 224 236 226 C90735 166 169 173 175 177 174 180 178 187 184 C90736 184 172 191 187 190 187 189 184 189 202 C90738 185 187 193 175 194 192 195 179 193 188 C90739 177 183 183 187 189 184 190 183 180 180 C90740 179 179 182 179 186 187 198 195 175 186 C90741 176 178 175 183 182 182 181 189 171 180 C90742 166 159 168 170 177 174 177 166 168 182 C90743 189 186 185 195 197 191 205 204 187 209 C90744 202 203 203 205 207 204 213 203 198 207 C90745 162 195 181 166 191 185 203 197 197 191 C90746 200 159 243 NOT TAKEN C90747 165 169 168 177 177 180 190 170 183 215 C90748 191 189 185 NOT TAKEN 1335 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 30 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C90749 168 165 162 167 168 165 168 171 171 188 C90750 161 157 169 163 164 168 167 168 169 172 C90751 201 SPILLED 209 SPILLED 215 SPILLED 219 207 225 219 C90752 207 208 206 203 204 203 195 192 192 204 C90754 207 198 192 203 199 195 200 195 195 210 C90755 213 197 209 213 219 214 228 219 218 238 C90757 188 183 185 188 190 186 192 196 197 195 C90758 216 236 232 SPILLED 255 SPILLED SPILLED 241 SPILLED 203 C90759 174 170 173 170 174 171 165 159 162 175 C90760 202 199 194 165 202 204 190 188 189 194 C90761 181 173 174 172 177 183 175 178 173 175 C90762 169 169 179 175 173 172 169 173 159 160 C90763 196 196 192 199 195 190 199 194 192 181 C90764 184 172 173 173 174 168 172 162 168 178 C90765 202 192 199 199 205 205 202 198 203 208 C90766 178 170 179 155 175 180 188 176 177 206 C90767 183 180 189 195 203 188 193 197 196 190 C90768 174 176 178 NOT TAKEN C90770 SPILLED 185 190 182 184 187 186 188 178 174 C90771 174 163 179 179 175 191 189 176 178 186 C90772 188 187 187 180 182 183 187 187 188 190 C90773 188 184 190 182 182 190 193 187 191 203 C90774 176 175 180 175 179 177 177 177 188 174 C90776 198 200 207 205 206 204 209 203 204 213 C90777 206 209 210 206 212 208 217 203 211 208 C90778 173 173 176 169 171 172 182 181 182 187 C90779 180 182 183 182 190 190 191 194 197 194 C90780 206 210 210 NOT TAKEN 1336 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 31 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 3 PPM C90781 187 187 188 190 188 177 182 183 177 182 C90782 211 180 185 183 183 184 179 181 108 186 C90783 170 154 158 157 165 164 164 167 163 170 C90784 176 169 181 181 173 176 186 176 178 183 C90785 184 189 197 198 201 192 201 200 194 198 C90786 163 165 173 175 175 158 176 176 175 187 C90787 197 197 190 190 193 190 205 186 185 191 C90788 180 180 182 178 184 189 189 186 193 195 C90790 207 208 204 196 205 201 223 205 198 222 C90791 169 189 168 149 179 173 181 177 167 184 C90792 184 181 187 187 160 143 199 154 145 191 C90793 174 174 171 168 180 178 172 169 170 177 C90794 177 186 200 198 201 215 211 200 106 28 C90795 187 198 193 184 185 179 181 177 170 186 C90796 204 SPILLED 214 211 198 192 191 195 194 204 C90797 173 172 174 NOT TAKEN C90798 176 173 175 179 176 177 197 189 189 188 C90799 163 165 166 165 167 173 181 170 154 176 C90800 183 182 194 181 188 188 192 190 185 193 C90801 190 190 198 194 200 189 197 199 173 194 C90802 148 149 157 146 157 161 162 167 165 182 C90803 189 191 191 193 202 198 198 194 195 197 C90804 205 200 204 199 210 196 202 190 204 205 C90805 186 181 195 198 200 204 205 205 195 203 C90806 158 164 159 159 165 167 172 172 181 186 C90807 170 175 180 NOT TAKEN C90808 200 160 SPILLED 202 200 201 204 206 194 216 C90809 174 181 183 179 177 183 190 182 177 180 C90810 197 190 199 196 195 197 197 205 174 195 C90811 195 192 190 193 190 193 193 196 186 182 C90812 173 171 178 178 179 185 188 179 185 197 1337 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 32 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 3 PPM C90815 187 187 183 181 187 181 189 182 171 183 C90816 198 197 197 181 200 198 201 201 197 211 C90818 205 196 212 NOT TAKEN C90819 180 172 165 167 183 191 181 190 189 198 C90820 172 170 180 148 176 175 176 171 177 179 C90821 198 198 199 200 203 205 214 203 207 220 C90822 162 153 162 167 171 172 174 173 174 190 C90823 178 173 183 185 186 188 190 180 186 179 C90824 176 166 171 180 178 177 182 180 181 180 C90825 194 183 185 189 196 186 194 186 185 202 C90826 234 222 SPILLED SPILLED 250 244 248 240 160 SPILLED C90827 191 182 183 179 181 186 201 193 192 206 C90828 204 205 219 224 210 221 215 216 214 210 C90829 196 185 196 204 203 208 206 219 206 219 C90830 195 184 183 185 198 190 193 190 101 197 C90831 199 196 205 NOT TAKEN C90832 191 182 186 182 188 181 189 184 185 188 C90835 193 180 173 173 179 185 185 182 177 184 C90836 185 185 190 NOT TAKEN C90837 166 160 168 169 162 163 168 164 161 163 C90838 217 219 221 223 236 228 237 226 136 235 C90839 212 211 212 214 208 217 233 220 152 219 C90840 192 191 188 168 203 199 205 205 190 195 GROUP: MALE 3 - 30 PPM C90841 182 177 174 177 174 177 180 190 185 191 C90842 145 142 145 143 142 138 150 147 146 160 C90843 187 176 176 NOT TAKEN C90844 SPILLED 205 205 214 214 230 220 217 107 224 C90845 180 171 171 178 176 173 186 187 177 176 1338 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 33 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90847 171 164 167 160 167 173 182 181 173 191 C90848 186 193 184 191 196 196 198 192 205 198 C90849 194 189 189 185 183 190 188 191 184 201 C90850 161 156 157 155 154 154 159 159 159 163 C90851 185 167 170 149 172 166 179 171 167 177 C90852 178 183 182 NOT TAKEN C90854 189 183 191 SPILLED SPILLED 199 SPILLED 212 SPILLED 226 C90855 182 186 198 187 187 189 191 185 168 188 C90856 181 169 174 145 168 169 191 184 170 190 C90857 178 179 184 176 184 187 193 186 177 183 C90858 157 149 152 159 157 163 165 170 168 174 C90859 194 193 203 194 190 189 204 198 181 214 C90860 151 165 167 162 169 164 173 157 157 166 C90861 182 180 186 174 182 189 191 187 186 191 C90862 152 151 156 152 155 158 169 164 157 168 C90863 199 192 200 NOT TAKEN C90864 162 162 170 163 169 168 181 173 163 178 C90865 149 149 153 151 155 154 162 163 160 173 C90867 158 157 164 160 159 159 163 166 173 174 C90868 198 197 197 196 200 204 207 194 191 201 C90869 180 171 178 168 171 177 181 174 186 193 C90870 182 180 186 178 192 165 191 190 190 199 C90871 153 155 157 152 161 160 162 158 150 164 C90872 164 164 170 175 170 176 180 181 190 175 C90873 190 177 191 197 207 188 205 194 204 198 C90874 190 196 194 184 189 192 189 189 191 193 C90875 168 169 180 173 187 182 185 183 170 182 C90876 205 193 195 184 178 175 201 206 196 194 C90877 170 170 172 NOT TAKEN C90878 168 173 176 174 172 176 184 180 177 169 C90879 182 176 179 178 178 183 191 205 196 195 1339 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 34 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90880 202 194 205 NOT TAKEN C90881 185 185 192 188 182 191 191 199 195 191 C90882 181 172 179 180 178 183 179 186 184 187 C90883 SPILLED 217 191 SPILLED 210 207 201 210 205 186 C90884 186 179 SPILLED 177 SPILLED SPILLED SPILLED 200 192 186 C90885 170 165 167 169 170 173 191 182 176 185 C90886 199 189 192 190 188 194 209 198 194 214 C90887 186 175 179 185 SPILLED 196 197 201 193 215 C90888 167 168 171 82 209 190 181 169 171 185 C90889 157 149 146 148 150 160 162 161 160 177 C90892 149 140 143 127 147 142 147 142 145 151 C90893 187 172 172 175 175 180 187 178 177 196 C90894 178 167 170 182 176 180 184 185 178 193 C90895 195 183 198 182 183 177 179 176 175 189 C90896 SPILLED 187 198 178 SPILLED 178 195 186 176 190 C90897 190 182 190 144 191 202 208 201 190 203 C90898 174 165 162 168 162 164 183 179 167 177 C90899 157 147 162 150 160 158 160 170 157 173 C90900 174 170 175 171 177 185 187 180 179 187 GROUP: MALE 4 - 100 PPM C90901 149 151 151 157 163 159 172 166 170 177 C90902 151 153 160 158 160 161 168 164 168 172 C90903 145 142 146 142 147 154 152 155 151 148 C90904 160 150 159 157 156 154 160 167 156 159 C90905 160 160 163 NOT TAKEN C90906 174 173 172 175 178 177 187 189 195 204 C90907 154 147 155 NOT TAKEN C90908 166 171 177 175 183 178 184 185 190 179 C90909 191 191 192 194 192 194 218 216 199 198 1340 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 35 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90910 170 160 170 167 173 172 178 178 C90911 175 165 175 175 176 175 183 176 C90912 170 168 175 171 169 171 190 176 C90913 177 162 158 176 170 178 184 179 C90914 SPILLED SPILLED 195 189 188 188 200 197 C90915 185 183 181 190 193 191 186 194 C90916 149 146 150 154 158 156 162 166 C90917 168 169 170 NOT TAKEN C90918 178 180 183 181 181 173 188 176 C90919 175 175 171 173 171 177 183 182 C90920 162 161 167 163 160 168 181 171 C90921 171 171 168 NOT TAKEN C90922 155 155 163 164 162 166 179 174 C90923 171 166 167 171 173 170 182 173 C90924 190 186 187 187 176 181 199 179 C90925 157 156 157 160 163 165 169 164 C90926 172 176 170 177 179 187 193 188 C90927 162 161 166 165 167 169 173 178 C90928 147 146 154 153 154 162 162 162 C90929 148 143 105 152 162 160 161 160 C90930 178 183 180 178 180 177 170 174 C90931 166 160 168 168 168 169 178 175 C90932 149 152 156 159 162 152 163 173 C90933 173 175 173 183 186 182 194 189 C90934 162 157 158 145 171 166 162 166 C90936 166 169 169 170 171 170 119 184 C90937 156 153 154 159 154 131 181 175 C90938 163 157 159 153 163 166 173 180 C90940 192 188 189 187 188 186 202 203 C90941 178 168 176 169 173 180 193 204 C90943 180 197 SPILLED 220 SPILLED 188 SPILLED 192 170 175 173 175 160 188 182 176 192 197 206 202 170 175 182 193 181 170 173 173 172 177 181 191 175 158 176 173 187 202 171 211 157 161 167 173 178 176 173 181 169 178 182 182 165 181 173 182 188 180 183 190 208 200 191 191 188 SPILLED 1341 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 36 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90944 141 C90945 149 C90946 140 C90947 161 C90948 162 C90949 75 C90950 171 C90951 169 C90952 180 C90953 159 C90954 154 C90955 166 C90956 145 C90957 168 C90958 161 C90959 142 C90960 165 C90962 185 C90963 171 C90964 186 C90965 143 C90967 166 C90968 162 C90969 206 C90970 160 143 142 138 147 146 152 153 155 158 152 148 153 154 153 156 159 162 170 154 166 161 165 164 180 174 164 153 158 161 153 165 172 177 175 169 181 166 170 172 175 175 184 188 181 173 55 NOT TAKEN 168 174 167 174 180 182 179 180 190 168 168 173 165 173 174 167 172 175 171 173 170 169 186 192 183 189 202 160 159 140 164 162 160 156 158 169 150 153 165 160 159 164 165 166 183 157 161 163 171 168 172 178 177 180 148 146 162 163 164 178 167 172 164 149 160 150 168 167 103 152 161 176 128 179 176 176 187 189 SPILLED 179 184 155 160 160 170 171 181 170 176 177 163 173 NOT TAKEN 179 179 177 181 181 181 187 182 193 173 176 183 181 179 189 182 187 176 188 184 184 192 197 209 206 202 199 144 80 152 SPILLED 161 SPILLED 175 149 SPILLED 161 168 167 169 165 168 166 172 180 164 166 151 169 171 177 173 165 181 204 209 209 201 204 215 212 228 222 153 158 158 163 162 170 161 161 156 GROUP: MALE 6 - 100 PPM C91041 188 181 184 180 186 187 186 185 183 186 C91042 165 171 177 165 167 176 181 173 185 192 C91043 180 174 181 175 172 177 180 188 185 184 1342 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 37 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 100 PPM C91044 184 179 181 177 180 180 187 194 194 198 C91045 181 186 184 182 176 185 189 190 190 184 C91047 157 162 156 165 161 154 160 164 158 138 C91048 179 176 186 172 177 188 189 198 187 183 C91049 197 189 191 189 199 202 214 222 204 213 C91050 192 191 191 189 193 197 208 192 191 206 C91051 194 191 195 192 192 190 206 205 200 206 C91052 203 200 SPILLED 193 207 204 219 211 205 214 C91053 159 161 166 168 169 170 174 176 171 169 C91054 163 161 169 162 167 187 189 181 169 188 C91055 189 183 186 193 201 192 203 197 199 199 C91056 183 188 188 199 196 195 187 197 SPILLED SPILLED C91057 166 170 165 168 172 179 171 174 167 176 C91058 143 141 149 153 157 153 154 158 154 167 C91059 175 172 172 173 171 164 172 173 178 171 C91060 167 157 164 162 170 168 176 165 162 165 C91061 174 180 188 188 181 184 186 187 172 175 C91062 172 168 173 174 175 181 176 180 186 SPILLED C91063 131 143 143 140 141 140 146 135 141 152 C91064 162 165 168 176 178 180 178 183 181 178 C91065 158 158 169 164 168 168 157 167 164 151 C91066 171 172 177 178 176 171 181 182 176 182 C91067 173 171 174 170 172 175 176 181 184 196 C91068 53 NOT TAKEN C91069 191 185 178 175 179 180 194 182 193 202 C91070 156 151 150 156 165 162 161 170 161 178 C91071 196 190 199 193 198 191 192 194 195 199 C91072 150 145 153 148 149 151 147 150 149 150 C91073 195 191 194 199 192 201 186 196 189 182 C91074 151 148 157 150 149 153 163 156 157 167 C91075 178 174 181 169 176 175 188 192 189 SPILLED 1343 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 38 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 100 PPM C91076 173 175 168 171 172 167 192 176 180 183 C91077 167 164 169 173 177 177 176 161 177 188 C91078 145 145 148 148 143 156 163 152 150 163 C91079 172 168 170 172 167 174 182 172 178 178 C91080 170 169 176 170 168 168 181 180 173 175 GROUP: FEMALE 1 - 0 PPM C91121 145 139 137 144 151 144 149 136 157 150 C91122 145 122 106 106 140 128 113 147 135 129 C91123 117 116 108 113 112 114 131 118 130 127 C91124 133 120 117 112 126 125 129 120 125 133 C91125 104 106 107 113 109 106 119 116 111 117 C91127 123 121 115 114 123 119 121 124 136 129 C91128 152 146 135 149 144 145 148 161 154 169 C91129 136 147 151 NOT TAKEN C91130 122 125 132 125 124 126 129 105 124 115 C91131 SPILLED 138 182 172 SPILLED SPILLED 135 113 128 110 C91132 SPILLED SPILLED SPILLED 171 SPILLED SPILLED SPILLED SPILLED 185 190 C91133 146 134 126 132 130 127 135 140 124 135 C91134 138 120 135 144 134 139 146 149 151 120 C91136 143 135 137 143 151 131 161 150 165 155 C91137 95 91 91 101 98 91 94 101 101 100 C91138 111 111 113 109 114 112 SPILLED 145 SPILLED 155 C91139 SPILLED 141 138 162 134 SPILLED SPILLED 125 126 153 C91141 145 129 116 128 140 123 126 126 123 124 C91142 139 115 127 128 128 126 142 150 137 152 C91144 113 108 101 102 107 102 106 108 106 113 C91146 124 125 121 126 124 121 137 132 138 146 C91147 136 149 163 152 SPILLED 150 SPILLED 145 SPILLED 182 C91148 135 126 112 103 119 124 129 106 127 140 1344 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 39 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C91149 C91150 C91151 C91152 C91153 C91154 C91155 C91156 C91157 C91158 C91159 C91160 C91161 C91164 C91166 C91167 C91168 C91170 C91172 C91173 C91174 C91175 C91176 C91177 C91178 C91179 C91180 C91181 C91182 C91183 C91184 SPILLED 126 152 SPILLED 128 141 126 131 122 107 105 124 132 122 130 126 SPILLED SPILLED 118 119 117 174 146 105 135 136 123 133 137 SPILLED 145 SPILLED 119 147 133 134 130 132 141 128 107 111 132 129 128 122 127 SPILLED SPILLED 122 119 119 164 SPILLED 105 144 127 132 131 141 SPILLED 137 130 116 141 122 118 117 119 128 108 108 111 130 131 122 117 119 SPILLED SPILLED 116 114 109 163 126 102 120 120 133 130 134 SPILLED 117 135 123 139 136 134 130 NOT TAKEN 128 110 112 107 NOT TAKEN 133 113 120 119 138 SPILLED 114 115 NOT TAKEN 171 123 108 117 130 144 NOT TAKEN 112 SPILLED 134 SPILLED 130 134 126 117 131 139 125 111 101 117 127 120 113 SPILLED SPILLED 122 115 163 136 120 140 128 146 133 SPILLED 129 SPILLED 123 148 131 121 131 145 121 124 103 139 120 126 121 127 SPILLED 121 121 163 132 118 136 130 151 137 SPILLED 138 SPILLED 123 157 109 138 137 147 135 134 112 133 134 143 141 130 154 SPILLED 122 165 147 110 144 142 160 142 SPILLED 138 149 133 158 SPILLED 159 127 150 129 122 107 132 147 127 144 119 155 146 116 156 137 113 120 139 171 145 153 131 SPILLED 129 167 121 159 154 168 120 125 102 127 176 131 152 121 139 156 139 162 135 114 152 153 162 151 SPILLED 134 136 127 181 127 172 165 156 140 120 120 122 155 128 166 120 138 170 146 167 136 114 146 154 160 137 SPILLED 124 1345 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 40 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C91185 146 138 128 124 138 130 139 141 144 131 C91186 131 120 120 122 121 122 129 125 121 128 C91187 138 139 129 130 129 133 136 138 142 141 C91188 115 120 115 122 119 114 117 124 123 136 C91189 103 101 106 104 105 107 108 106 111 112 C91190 211 125 127 SPILLED 133 126 147 152 137 159 GROUP: FEMALE 2 - 3 PPM C91191 120 125 112 131 128 112 154 146 149 149 C91192 122 115 116 NOT TAKEN C91193 SPILLED SPILLED SPILLED 128 124 SPILLED 162 147 SPILLED 125 C91194 137 132 131 137 127 133 134 147 148 148 C91195 136 127 129 128 131 120 149 155 155 154 C91196 104 108 99 94 106 107 110 122 119 133 C91197 150 141 SPILLED 144 SPILLED 141 143 158 148 161 C91199 SPILLED 150 SPILLED 201 234 157 SPILLED 126 130 143 C91200 178 124 128 118 118 SPILLED 127 117 124 120 C91201 SPILLED 135 153 134 120 119 151 132 146 135 C91202 124 113 115 115 128 126 114 130 137 134 C91203 119 116 120 129 134 134 129 136 128 138 C91204 141 138 132 113 138 137 138 128 119 143 C91206 135 121 122 135 137 131 152 173 166 163 C91207 133 124 132 123 128 127 127 130 139 141 C91208 119 123 111 124 SPILLED 124 SPILLED 134 141 134 C91209 106 104 109 122 111 118 107 123 130 144 C91210 SPILLED SPILLED 208 179 SPILLED SPILLED SPILLED SPILLED 179 181 C91211 115 124 118 121 121 124 132 130 152 124 C91212 119 116 121 117 112 115 128 128 128 133 C91213 130 114 119 132 132 125 128 139 106 141 C91214 130 106 114 121 134 133 120 151 128 125 1346 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 41 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91215 C91216 C91217 C91218 C91219 C91220 C91221 C91223 C91224 C91225 C91226 C91227 C91228 C91229 C91231 C91232 C91233 C91234 C91235 C91236 C91238 C91239 C91240 C91241 C91242 C91243 C91244 C91245 C91246 C91247 C91248 102 162 123 SPILLED SPILLED 120 128 100 132 112 128 121 127 103 124 114 119 145 SPILLED 112 SPILLED 125 100 SPILLED 216 168 110 151 SPILLED SPILLED 131 109 SPILLED 121 SPILLED SPILLED 115 145 113 115 108 126 119 125 107 118 122 119 144 SPILLED 112 158 122 92 126 SPILLED SPILLED 105 SPILLED SPILLED SPILLED SPILLED 107 126 114 SPILLED SPILLED 108 139 111 112 109 136 112 132 115 121 113 111 151 SPILLED 107 104 124 122 129 SPILLED 150 104 144 SPILLED 207 115 102 128 113 SPILLED SPILLED 110 117 NOT TAKEN 114 113 128 111 106 106 124 106 NOT TAKEN 140 SPILLED 110 104 123 129 NOT TAKEN SPILLED 191 107 146 SPILLED 180 NOT TAKEN 111 138 119 SPILLED SPILLED 119 140 112 104 135 118 124 105 129 SPILLED 146 SPILLED 108 105 129 115 SPILLED SPILLED 108 SPILLED SPILLED SPILLED 120 125 114 SPILLED SPILLED 115 138 118 115 137 111 132 108 149 119 150 SPILLED 113 113 115 120 SPILLED 164 111 147 SPILLED 178 118 121 128 SPILLED SPILLED 115 160 115 102 147 110 121 125 125 130 150 183 123 105 139 117 SPILLED SPILLED SPILLED 141 SPILLED 182 129 131 118 142 SPILLED 134 143 123 137 148 109 139 115 120 121 146 155 147 121 130 153 224 188 127 163 222 SPILLED 131 138 130 141 177 122 129 126 108 148 118 135 117 133 114 140 159 139 115 137 118 SPILLED 194 130 136 SPILLED SPILLED 148 137 132 SPILLED 140 136 158 138 126 154 122 133 116 142 116 181 143 154 137 144 122 221 174 135 139 SPILLED 189 1347 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 42 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91249 145 135 131 124 135 136 146 141 149 145 C91250 107 100 138 100 103 106 105 102 101 105 GROUP: FEMALE 3 - 30 PPM C91252 137 126 123 139 133 134 145 129 149 148 C91253 119 123 125 114 124 118 133 121 132 131 C91254 119 108 111 106 113 111 107 104 117 117 C91255 130 126 128 117 118 126 120 126 136 141 C91256 123 122 118 114 116 117 129 121 119 118 C91257 SPILLED SPILLED 156 SPILLED 144 131 97 SPILLED SPILLED SPILLED C91258 124 116 114 112 123 110 129 119 132 126 C91259 SPILLED 122 117 121 124 127 134 135 146 142 C91260 136 121 119 130 143 134 144 147 140 162 C91261 118 125 122 121 122 133 136 141 133 137 C91262 92 97 94 88 104 97 115 111 111 103 C91263 130 127 128 131 137 140 152 150 156 160 C91264 102 116 106 104 124 112 128 111 118 126 C91265 111 129 106 110 116 113 110 112 116 126 C91266 141 137 123 131 130 125 131 172 151 164 C91267 105 98 97 103 100 105 105 121 SPILLED 122 C91268 109 107 100 94 105 102 104 118 115 124 C91269 142 148 147 130 135 SPILLED 136 122 151 145 C91270 139 113 109 124 122 127 131 127 121 132 C91271 195 145 141 142 140 135 141 145 127 138 C91273 153 156 157 156 SPILLED SPILLED 149 141 136 142 C91274 118 115 112 125 SPILLED 125 117 127 124 131 C91275 SPILLED 133 122 149 135 133 134 154 141 146 C91276 122 114 101 110 118 113 120 118 129 136 C91277 118 111 113 118 114 114 110 117 122 128 C91278 SPILLED 118 115 121 120 118 132 133 132 135 1348 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 43 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 30 PPM C91279 C91280 C91281 C91282 C91283 C91284 C91286 C91288 C91289 C91290 C91292 C91293 C91294 C91295 C91296 C91297 C91298 C91299 C91300 C91301 C91302 C91303 C91304 C91305 C91306 C91307 C91308 C91309 C91310 159 115 SPILLED 139 122 127 113 105 120 131 122 179 112 129 SPILLED 140 112 102 228 115 179 123 144 148 116 118 177 123 120 163 130 SPILLED 145 114 SPILLED 119 109 109 131 120 137 117 129 165 154 138 134 137 120 SPILLED 137 151 149 126 118 168 129 128 147 126 172 140 111 139 115 107 103 122 114 133 117 115 122 134 141 100 144 119 SPILLED 118 148 146 122 109 208 134 125 165 126 NOT TAKEN 150 115 SPILLED 109 NOT TAKEN 112 148 120 NOT TAKEN 116 130 123 SPILLED SPILLED NOT TAKEN 138 115 121 133 NOT TAKEN 134 117 121 SPILLED 136 122 162 134 144 105 134 SPILLED 111 158 130 119 SPILLED SPILLED 170 SPILLED SPILLED 120 114 141 157 127 113 SPILLED 144 124 165 126 147 114 129 SPILLED 109 162 129 115 125 SPILLED 144 SPILLED SPILLED 118 120 131 159 117 114 208 141 125 208 135 148 124 153 SPILLED 119 178 135 121 130 124 162 SPILLED SPILLED 127 124 142 170 124 127 179 147 124 174 138 143 129 145 SPILLED 122 192 120 118 115 114 171 SPILLED 166 137 137 131 163 124 133 175 148 130 177 134 158 130 141 SPILLED 130 139 129 127 136 121 SPILLED 117 152 118 135 125 167 121 122 SPILLED 145 123 189 133 152 122 126 142 126 211 120 152 129 120 196 SPILLED 152 121 145 158 180 149 138 159 156 122 1349 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 44 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91311 C91312 C91313 C91314 C91315 C91318 C91319 C91320 C91321 C91322 C91323 C91324 C91325 C91326 C91327 C91328 C91329 C91330 C91331 C91332 C91334 C91335 C91336 C91337 C91338 C91339 C91340 C91341 C91342 C91343 C91344 97 101 144 145 124 94 123 111 125 125 116 112 102 104 110 108 105 108 127 120 108 107 SPILLED 115 92 106 117 112 110 117 107 107 100 115 SPILLED 113 97 117 113 127 125 120 151 101 102 98 113 102 104 112 121 114 103 SPILLED 120 85 113 112 108 112 116 103 124 101 117 SPILLED 113 90 117 109 113 124 122 99 100 92 106 97 101 104 116 110 109 104 162 113 91 103 105 105 103 116 100 105 103 117 SPILLED 109 95 116 118 128 122 129 107 108 89 106 109 NOT TAKEN 106 122 93 114 110 SPILLED NOT TAKEN 91 108 108 110 107 116 113 105 105 123 SPILLED 122 NOT TAKEN 122 116 129 128 129 106 106 109 107 112 116 126 SPILLED 109 104 SPILLED 97 114 107 112 106 120 109 113 104 118 SPILLED 117 121 110 119 138 129 108 110 97 110 104 110 125 108 109 109 SPILLED 100 110 114 109 115 109 110 124 106 124 SPILLED 121 125 124 131 135 146 107 110 111 119 SPILLED 118 130 107 112 113 SPILLED 97 120 112 121 116 128 107 121 107 129 SPILLED 120 125 SPILLED 139 143 113 111 117 113 133 123 135 107 120 108 SPILLED 94 118 132 135 120 129 111 131 111 133 SPILLED 123 139 123 142 153 108 111 118 113 119 120 152 103 119 105 SPILLED 90 131 114 133 109 134 110 133 115 143 SPILLED 129 139 125 150 157 111 120 118 119 112 134 151 139 124 110 187 95 137 131 133 118 130 119 1350 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 45 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91346 127 134 130 137 136 146 143 138 149 144 C91347 106 112 106 110 112 119 109 120 118 122 C91348 97 98 96 89 102 105 103 104 107 113 C91349 110 108 111 118 119 117 122 128 154 150 C91351 129 122 121 130 122 134 125 131 119 138 C91352 118 108 115 117 128 128 138 130 133 142 C91353 108 112 108 117 111 112 118 127 124 126 C91354 95 96 92 97 93 102 SPILLED 100 102 106 C91355 153 137 116 NOT TAKEN C91356 131 119 113 112 123 119 143 146 140 SPILLED C91357 94 84 85 84 87 88 96 86 94 93 C91358 130 114 120 118 126 131 133 133 126 147 C91359 SPILLED 142 176 135 136 111 121 127 137 140 C91360 103 101 102 103 106 104 112 118 115 123 C91361 87 102 106 103 105 101 110 114 108 104 C91362 141 150 116 SPILLED 112 109 135 135 123 129 C91363 120 126 124 131 126 117 136 130 117 152 C91364 99 99 95 102 94 98 104 102 107 116 C91365 232 SPILLED 228 228 SPILLED SPILLED SPILLED 215 SPILLED SPILLED C91366 116 103 106 122 110 113 121 122 126 140 C91367 125 114 113 125 118 115 130 141 135 139 C91368 103 107 102 96 102 101 107 106 106 107 C91369 118 109 124 119 120 126 SPILLED 130 129 137 C91370 110 110 110 NOT TAKEN C91371 SPILLED 155 145 120 126 111 134 SPILLED 144 143 C91372 116 120 113 106 114 115 121 114 107 177 C91373 98 92 107 109 109 115 115 132 142 140 C91374 109 115 107 95 118 113 117 119 109 119 C91375 111 110 114 112 110 109 112 118 115 121 C91376 104 102 98 102 105 104 106 108 108 114 C91377 124 123 115 113 128 125 135 140 133 136 1351 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 46 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91378 115 99 98 111 111 105 112 114 112 108 C91379 113 122 117 NOT TAKEN C91380 124 128 111 126 126 119 132 128 124 128 GROUP: FEMALE 6 - 100 PPM C91451 C91452 C91453 C91454 C91455 C91456 C91457 C91458 C91459 C91460 C91461 C91462 C91463 C91464 C91465 C91466 C91467 C91468 C91469 C91470 C91471 C91472 C91473 C91474 C91475 113 119 114 109 116 101 117 105 SPILLED 157 126 131 96 106 113 125 110 94 106 133 108 SPILLED 111 SPILLED 139 110 121 121 102 109 101 125 99 SPILLED 97 110 125 100 100 115 105 107 104 103 138 107 SPILLED 174 163 121 111 118 115 100 103 100 128 104 SPILLED 89 102 122 91 102 111 113 99 99 101 139 107 123 159 118 120 108 115 131 115 102 103 121 98 SPILLED 96 115 133 100 99 111 112 110 98 109 134 112 112 SPILLED 124 120 108 117 126 112 105 104 124 112 SPILLED 95 118 128 108 104 112 112 107 92 102 142 116 136 107 131 129 114 122 124 104 112 102 128 106 SPILLED 90 133 127 107 98 118 114 103 SPILLED 100 134 114 123 94 127 127 119 123 121 111 115 108 135 117 SPILLED 108 145 133 108 124 106 123 115 SPILLED 108 137 119 122 104 146 138 122 129 125 109 115 114 128 115 SPILLED 98 154 136 109 107 134 121 115 SPILLED 111 144 127 119 119 132 139 125 129 134 118 111 111 125 118 SPILLED 101 155 131 115 115 139 116 122 109 109 135 128 117 102 153 133 126 146 130 120 118 116 133 125 SPILLED 104 153 143 117 115 148 125 130 153 122 151 130 121 SPILLED 127 142 1352 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 47 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 100 PPM C91476 111 111 112 115 108 114 128 130 137 131 C91477 123 115 112 124 121 122 122 135 139 143 C91478 134 138 121 125 136 146 137 144 146 SPILLED C91479 133 133 126 132 130 138 144 138 144 SPILLED C91480 111 105 103 109 105 104 109 111 120 130 C91481 92 91 93 95 102 96 100 102 101 110 C91482 116 112 110 122 118 111 117 120 118 138 C91483 107 107 98 108 109 120 133 115 122 133 C91484 110 106 107 111 113 106 95 110 124 124 C91485 105 103 96 83 98 96 109 111 116 116 C91486 107 109 104 114 112 112 118 122 134 130 C91487 111 105 96 99 100 98 115 125 124 132 C91488 124 121 129 121 128 116 125 122 123 126 C91489 136 117 120 118 119 129 141 127 141 130 C91490 117 113 114 111 116 112 121 118 119 123 1353 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 48 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C90711 192 199 182 188 176 174 179 183 177 103 C90712 184 200 160 186 185 188 191 178 176 203 C90713 228 228 225 229 210 191 203 208 212 221 C90714 187 205 190 189 186 C90715 189 204 192 198 187 189 193 190 198 217 C90716 170 182 168 163 170 170 169 178 181 190 C90720 198 210 191 186 186 165 187 183 199 203 C90721 244 69 NOT TAKEN C90722 176 187 176 173 175 171 172 170 180 192 C90723 214 233 212 218 207 196 201 C90724 180 196 183 180 188 191 198 193 190 197 C90726 183 189 179 179 176 182 181 177 185 183 C90727 201 205 192 194 189 187 190 204 204 206 C90728 190 207 187 201 197 191 199 192 189 194 C90729 189 188 185 170 175 175 180 180 187 187 C90730 208 214 207 211 207 208 181 63 45 C90733 204 215 202 200 203 215 SPILLED 224 211 207 C90734 231 SPILLED 242 235 226 218 228 201 232 218 C90735 186 200 179 181 179 176 177 159 183 190 C90736 203 221 199 197 200 195 202 172 117 NOT TAKEN C90738 185 196 193 191 185 187 186 182 183 163 C90739 184 192 186 173 187 185 186 169 185 177 C90740 207 211 207 204 209 181 155 67 C90741 189 192 187 187 184 186 178 180 182 200 C90742 183 192 173 172 165 172 180 170 176 177 C90743 209 225 208 200 205 204 210 210 212 201 C90744 211 226 217 210 218 203 216 213 220 223 C90745 219 233 213 211 217 202 202 195 193 198 C90747 202 206 183 191 187 182 187 179 152 206 C90749 175 198 181 173 170 176 168 163 176 183 C90750 165 179 174 176 174 177 173 183 196 179 C90751 216 221 210 199 198 1354 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 49 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C90752 200 211 215 198 198 C90754 217 231 218 209 222 C90755 241 255 260 247 235 233 238 233 242 239 C90757 200 212 203 198 206 210 217 130 54 C90758 209 211 209 196 193 188 188 187 189 191 C90759 176 188 169 170 169 169 168 170 169 192 C90760 197 218 212 215 209 208 214 212 219 195 C90761 187 200 183 178 176 170 181 177 184 183 C90762 163 135 149 172 146 154 160 171 169 176 C90763 175 186 175 172 183 169 178 181 185 186 C90764 124 186 176 180 176 167 179 173 177 177 C90765 219 227 199 200 208 212 212 197 207 213 C90766 196 212 190 196 196 156 195 C90767 172 200 183 190 191 C90770 195 198 201 188 189 185 183 186 197 185 C90771 181 202 191 184 183 175 177 174 185 190 C90772 186 205 196 188 191 150 103 C90773 200 220 203 210 216 213 223 143 C90774 176 182 171 177 178 166 178 174 67 NOT TAKEN C90776 215 230 222 211 214 214 221 224 231 237 C90777 219 240 219 220 207 222 203 220 227 243 C90778 199 199 198 186 183 171 178 170 179 183 C90779 190 223 207 203 199 182 195 198 189 184 GROUP: MALE 2 - 3 PPM C90781 189 197 183 188 182 186 179 164 191 187 C90782 185 204 188 184 178 183 186 189 180 203 C90783 157 179 168 159 156 162 158 158 154 169 C90784 182 201 185 177 174 174 179 171 181 188 C90785 203 228 203 214 196 197 200 195 198 209 1355 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 50 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 3 PPM C90786 178 191 179 175 160 160 172 187 170 178 C90787 202 212 193 200 196 191 205 198 206 206 C90788 190 207 188 188 186 179 195 187 193 195 C90790 217 232 223 215 203 218 207 222 198 226 C90791 186 206 184 184 182 184 190 174 189 193 C90792 201 209 195 193 180 10 C90793 177 188 187 186 181 175 185 180 183 177 C90794 SPILLED SPILLED 222 213 195 195 206 191 194 212 C90795 184 187 180 173 102 20 C90796 199 216 208 202 200 170 C90798 198 211 190 194 190 195 199 190 217 217 C90799 171 178 169 168 168 163 169 169 171 178 C90800 190 208 197 193 194 198 198 205 210 215 C90801 203 222 207 165 231 209 213 205 201 209 C90802 189 192 178 187 179 171 177 163 182 191 C90803 203 223 204 202 201 195 206 SPILLED 222 243 C90804 208 214 204 200 194 194 198 204 199 197 C90805 208 207 195 192 218 50 18 C90808 210 230 215 198 202 201 219 34 32 C90809 187 205 178 187 192 187 187 183 186 189 C90810 201 226 212 210 200 190 216 210 217 222 C90811 194 205 182 227 210 185 154 179 189 195 C90812 191 215 200 193 189 178 188 193 206 199 C90815 181 205 189 180 191 168 183 174 182 182 C90816 209 218 193 203 222 211 227 204 211 221 C90819 193 220 211 201 203 193 201 210 211 228 C90820 183 196 186 183 174 178 184 195 76 C90821 222 232 221 228 206 205 215 219 222 219 C90822 191 201 181 175 177 170 173 170 171 150 C90823 180 198 184 188 171 179 171 174 167 196 C90824 189 214 193 190 184 180 192 187 188 196 1356 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 51 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 3 PPM C90825 192 210 197 192 210 194 193 189 206 200 C90826 267 SPILLED 234 239 238 230 230 135 221 230 C90827 208 220 211 221 223 230 214 228 NOT TAKEN C90828 205 216 210 230 251 191 204 208 212 201 C90829 234 239 228 219 219 209 216 211 221 239 C90830 183 205 185 181 180 174 178 186 186 195 C90832 195 219 187 203 202 182 191 190 201 62 C90835 175 181 184 189 197 174 189 192 207 190 C90837 169 174 159 160 187 238 275 229 SPILLED 306 C90838 233 198 232 224 226 197 221 226 230 242 C90839 224 249 222 222 217 209 209 221 232 213 C90840 200 227 207 191 205 199 208 217 209 201 GROUP: MALE 3 - 30 PPM C90841 201 195 191 184 198 179 189 193 193 207 C90842 150 153 148 144 148 139 149 135 146 154 C90844 204 234 208 203 214 181 218 207 216 223 C90845 179 197 174 181 186 181 185 192 195 207 C90847 189 197 190 182 181 171 186 183 197 202 C90848 194 223 220 204 214 197 209 186 200 178 C90849 195 198 199 199 200 195 186 164 136 107 C90850 159 179 159 155 166 164 169 160 165 187 C90851 168 183 169 172 174 168 182 169 175 190 C90854 208 247 204 197 193 189 204 194 210 205 C90855 197 202 179 186 182 171 179 166 176 186 C90856 183 202 186 184 174 165 193 184 182 195 C90857 182 201 181 200 185 181 177 184 187 194 C90858 179 188 185 181 178 175 172 168 174 176 C90859 212 228 213 214 215 178 156 79 188 C90860 179 175 161 166 162 164 166 152 157 172 1357 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 52 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90861 199 193 191 184 189 182 197 131 76 C90862 166 177 167 162 162 163 163 74 93 C90864 187 195 179 176 173 174 168 181 180 175 C90865 174 181 168 174 173 162 172 163 175 169 C90867 170 192 175 166 171 174 176 173 170 193 C90868 202 219 203 193 207 206 196 196 193 59 C90869 198 214 202 198 205 202 206 204 161 224 C90870 226 234 199 203 202 204 205 205 195 165 C90871 162 179 166 162 169 163 174 160 167 170 C90872 174 196 180 176 175 173 179 177 178 187 C90873 198 204 189 181 SPILLED 193 186 SPILLED 167 139 C90874 200 214 202 195 200 31 231 225 C90875 182 197 189 190 186 177 196 188 195 206 C90876 SPILLED 235 218 210 216 NOT TAKEN C90878 170 183 164 165 172 C90879 207 205 199 207 185 187 198 201 216 197 C90881 197 216 205 200 197 188 202 198 232 228 C90882 193 203 188 193 196 187 194 192 193 207 C90883 207 239 226 225 152 223 217 177 195 184 C90884 198 205 195 171 185 190 200 SPILLED SPILLED SPILLED C90885 190 204 195 188 186 177 184 198 189 184 C90886 212 231 216 222 206 222 219 185 215 222 C90887 200 232 212 209 212 212 198 196 0 C90888 186 213 187 164 169 167 179 206 197 234 C90889 166 183 173 170 177 157 180 170 176 180 C90892 154 170 159 155 157 149 156 167 146 88 C90893 181 209 187 199 201 191 186 180 193 192 C90894 192 200 205 204 198 186 190 180 173 198 C90895 185 204 182 187 183 175 175 171 169 189 C90896 195 207 195 198 207 212 177 120 57 NOT TAKEN C90897 208 220 207 200 205 200 200 194 201 207 1358 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90898 178 195 166 176 174 191 30 C90899 172 187 170 171 178 179 177 168 168 170 C90900 189 204 189 185 187 184 178 169 170 177 GROUP: MALE 4 - 100 PPM C90901 172 186 176 175 181 185 178 190 196 118 C90902 171 181 169 173 173 C90903 157 170 163 162 151 153 158 155 168 166 C90904 170 184 170 162 163 158 160 162 161 161 C90906 207 222 206 197 194 193 186 175 195 208 C90908 173 193 167 173 183 190 179 192 194 197 C90909 207 212 215 204 199 C90910 172 184 171 163 163 157 158 167 168 173 C90911 177 188 182 176 181 175 171 167 176 177 C90912 192 205 188 190 188 187 185 102 127 116 C90913 165 181 164 170 159 C90914 190 196 188 183 194 193 186 197 212 210 C90915 208 211 208 206 211 206 202 204 215 218 C90916 174 186 174 175 176 170 161 166 173 178 C90918 203 202 196 191 180 C90919 189 190 193 188 192 175 173 174 188 192 C90920 172 190 176 182 188 192 195 184 200 218 C90922 189 193 182 176 176 155 100 NOT TAKEN C90923 202 189 197 188 191 196 191 189 196 212 C90924 168 180 188 184 187 C90925 186 197 195 179 183 186 184 179 194 198 C90926 211 219 216 215 220 191 189 180 190 205 C90927 183 194 162 167 170 164 167 158 172 182 C90928 166 179 157 156 155 169 163 159 157 172 C90929 177 178 147 150 144 182 149 174 NOT TAKEN 1359 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 54 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90930 184 190 177 168 177 177 173 164 185 208 C90931 184 201 192 186 168 175 179 176 165 183 C90932 194 202 187 176 174 C90933 192 198 176 180 146 189 170 182 153 184 C90934 183 187 182 172 197 187 194 183 140 194 C90936 177 160 190 184 154 178 182 114 151 195 C90937 189 229 196 197 183 189 187 180 178 200 C90938 192 197 191 183 188 176 187 184 36 C90940 208 237 217 232 218 220 117 NOT TAKEN C90941 197 219 185 201 205 201 207 212 200 220 C90943 212 203 SPILLED 181 181 205 204 SPILLED 186 185 C90944 156 168 161 159 149 152 160 155 150 161 C90945 172 174 NOT TAKEN C90946 125 187 121 154 177 189 153 171 186 182 C90947 175 200 178 178 182 170 173 177 167 181 C90948 179 196 174 170 177 172 170 173 171 175 C90950 191 217 194 202 184 C90951 177 188 167 170 176 C90952 209 229 202 191 155 74 C90953 159 170 164 162 168 167 166 167 174 175 C90954 184 196 186 182 178 168 168 120 69 C90955 184 195 183 181 182 180 182 190 194 192 C90956 180 204 179 184 182 188 189 187 161 187 C90957 177 165 166 181 176 C90958 197 155 182 192 173 192 94 200 211 212 C90959 192 205 193 187 186 184 179 190 185 196 C90962 203 217 199 201 192 199 194 177 76 C90963 183 200 182 183 188 185 173 71 86 86 C90964 207 214 199 197 201 234 213 136 127 SPILLED C90965 209 207 171 188 171 179 181 129 SPILLED SPILLED C90967 183 196 183 175 171 1360 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 55 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90968 181 199 173 172 172 159 170 177 2 224 C90969 213 230 231 225 216 211 216 202 221 207 C90970 163 173 172 157 168 154 170 157 181 173 GROUP: MALE 6 - 100 PPM C91041 197 211 210 204 201 134 2 C91042 188 190 180 191 175 183 200 194 204 205 C91043 194 205 189 178 170 186 190 186 185 198 C91044 194 213 193 200 196 147 85 NOT TAKEN C91045 200 214 196 157 C91047 162 189 150 170 169 169 183 173 179 186 C91048 193 208 199 204 204 201 200 200 196 198 C91049 212 45 C91050 189 213 192 190 195 206 205 200 199 203 C91051 213 218 199 212 207 207 210 202 202 225 C91052 229 237 226 229 240 248 175 45 NOT TAKEN C91053 172 182 172 182 179 194 187 179 188 195 C91054 192 203 194 191 198 193 217 213 223 226 C91055 225 211 218 206 198 216 224 202 233 247 C91056 193 SPILLED 189 187 183 184 176 192 186 195 C91057 183 197 189 177 170 185 199 203 175 127 C91058 169 183 175 166 168 155 182 175 167 191 C91059 167 186 168 187 196 188 191 201 206 202 C91060 169 181 167 172 165 172 188 185 196 206 C91061 174 187 169 174 172 171 187 189 184 196 C91062 215 199 184 193 183 185 199 190 194 190 C91063 155 151 143 144 144 140 158 154 153 150 C91064 188 202 199 188 187 171 185 198 194 193 C91065 169 178 158 152 161 126 148 138 135 158 C91066 182 188 182 184 180 189 206 190 180 170 1361 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 56 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 100 PPM C91067 199 202 185 186 186 201 202 196 199 198 C91069 213 226 201 208 201 219 228 216 217 254 C91070 172 188 184 172 163 171 183 182 185 179 C91071 188 198 186 192 184 184 207 208 213 220 C91072 154 172 166 158 157 160 140 158 167 160 C91073 194 208 206 209 214 222 194 185 236 228 C91074 165 177 180 171 163 166 180 180 182 184 C91075 194 206 200 202 191 193 194 200 199 206 C91076 178 193 175 187 185 173 194 198 199 197 C91077 196 207 197 182 180 185 188 151 188 204 C91078 165 150 141 167 168 141 170 174 176 174 C91079 172 193 177 188 182 188 196 204 189 158 C91080 186 185 174 178 183 185 182 173 196 198 GROUP: FEMALE 1 - 0 PPM C91121 151 162 151 134 158 C91122 139 135 131 150 152 C91123 132 148 138 136 128 140 118 144 141 144 C91124 142 148 160 142 144 C91125 129 145 135 135 135 116 128 137 142 151 C91127 118 155 132 137 147 138 159 151 150 171 C91128 161 196 193 170 180 167 144 179 217 196 C91130 127 150 160 130 138 151 130 148 154 143 C91131 111 118 107 107 103 111 114 116 118 123 C91132 201 174 154 147 167 158 146 141 141 122 C91133 163 144 134 139 142 C91134 170 175 182 165 171 184 170 169 29 59 C91136 170 173 168 175 168 159 168 158 153 183 C91137 110 113 104 85 100 91 116 115 125 134 C91138 168 182 124 129 119 116 117 108 123 113 1362 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 57 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C91139 140 143 166 142 148 149 36 C91141 144 149 162 149 138 134 124 126 154 127 C91142 137 155 155 143 141 134 119 138 134 0 C91144 109 114 114 SPILLED 108 112 112 111 109 111 C91146 148 168 154 143 156 135 99 149 116 72 C91147 205 SPILLED 188 167 163 160 160 173 152 105 C91148 134 129 145 143 134 135 143 136 155 159 C91149 140 156 164 142 156 138 155 148 139 137 C91150 158 170 160 162 150 164 141 114 122 122 C91151 187 222 208 204 207 188 187 184 116 15 C91152 151 153 149 148 145 C91153 174 205 178 196 193 207 198 198 200 185 C91154 138 151 150 140 134 146 130 136 134 129 C91156 153 171 200 179 181 194 191 176 170 148 C91157 147 143 124 123 127 121 123 124 128 117 C91158 163 164 163 134 132 131 149 147 162 168 C91159 126 136 126 114 123 137 121 128 125 130 C91161 148 162 134 142 136 62 7 C91164 171 190 188 185 151 192 184 181 188 190 C91166 128 140 142 136 138 131 144 142 142 139 C91167 156 185 168 156 144 164 156 141 184 172 C91168 125 141 141 136 146 140 134 142 160 163 C91170 159 169 163 143 179 159 130 133 140 142 C91172 200 SPILLED 202 171 171 159 170 145 176 167 C91173 110 169 159 153 117 64 C91175 156 SPILLED 188 146 153 166 146 171 156 191 C91176 150 155 155 139 148 160 145 160 171 185 C91177 103 147 114 115 120 119 120 150 167 170 C91178 153 160 154 141 141 134 137 137 140 150 C91179 153 168 138 C91180 145 154 162 178 181 SPILLED SPILLED NOT TAKEN 1363 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 58 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C91182 164 175 166 157 160 163 157 173 189 164 C91183 SPILLED SPILLED SPILLED 199 231 196 SPILLED 178 190 184 C91184 145 180 162 149 163 171 165 177 178 173 C91185 124 171 164 165 160 149 53 8 C91186 126 152 162 158 144 143 156 130 146 165 C91187 148 169 155 147 156 155 138 108 88 76 C91188 138 141 138 132 123 141 139 137 143 137 C91189 115 122 118 114 118 114 117 111 122 120 C91190 143 151 137 145 137 146 138 128 159 144 GROUP: FEMALE 2 - 3 PPM C91191 137 166 186 113 SPILLED 175 149 182 189 179 C91193 165 146 152 141 114 130 141 118 131 140 C91194 148 164 148 154 139 95 133 126 158 166 C91195 170 177 150 172 179 151 153 172 174 151 C91196 116 154 157 148 140 149 160 150 172 164 C91197 185 208 200 170 154 133 153 144 145 148 C91199 140 153 141 143 145 141 149 146 149 159 C91200 132 176 126 130 127 117 124 137 148 120 C91201 150 159 136 138 140 144 120 163 152 158 C91202 164 179 150 160 174 159 170 180 158 168 C91203 145 160 153 149 148 131 165 169 156 167 C91204 156 166 159 190 160 161 85 C91206 169 170 155 168 167 157 175 116 118 162 C91207 143 146 141 139 143 100 151 125 117 131 C91208 155 157 138 138 152 137 134 127 98 84 C91209 172 184 143 136 151 134 129 140 153 191 C91210 152 154 148 135 135 131 156 142 140 144 C91211 131 154 147 127 133 147 131 150 165 171 C91212 144 148 142 130 137 148 150 2 1364 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 59 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91213 C91214 C91215 C91216 C91217 C91218 C91219 C91220 C91221 C91224 C91225 C91226 C91227 C91228 C91229 C91231 C91232 C91234 C91235 C91236 C91238 C91239 C91240 C91242 C91243 C91244 C91245 C91246 C91247 C91249 C91250 125 162 146 143 121 166 SPILLED 132 159 143 141 147 128 144 121 134 124 140 SPILLED 162 149 144 143 192 261 99 146 SPILLED 179 163 123 160 172 158 143 143 188 227 153 169 169 142 167 136 154 135 154 136 171 181 168 147 132 135 SPILLED 208 142 149 SPILLED 203 173 128 155 148 120 137 145 192 168 137 174 143 126 150 126 154 125 174 120 186 152 155 141 146 110 SPILLED 153 131 158 SPILLED 194 165 114 154 147 115 125 121 171 161 129 160 112 133 148 128 146 126 165 127 186 144 147 145 135 139 147 176 130 158 SPILLED 180 162 117 161 148 164 119 139 139 144 128 142 132 131 151 130 149 127 147 111 170 123 148 138 141 SPILLED SPILLED 140 140 141 SPILLED 180 160 133 131 137 124 125 179 142 129 137 135 127 148 127 SPILLED 117 161 123 170 115 144 143 128 104 144 140 141 138 SPILLED 150 164 143 147 149 135 138 SPILLED 144 136 151 128 136 158 117 170 114 149 127 180 132 145 140 129 152 146 147 153 SPILLED 167 171 152 138 157 145 131 187 137 126 161 120 125 159 130 159 122 149 137 100 127 142 17 124 SPILLED 167 105 148 161 91 167 140 134 173 149 NOT TAKEN SPILLED 162 141 155 133 121 159 128 163 125 156 139 142 145 NOT TAKEN 141 SPILLED 166 147 175 117 184 155 76 185 149 SPILLED 147 156 159 141 136 151 144 182 150 158 143 151 156 144 146 160 171 SPILLED 70 170 154 1365 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 60 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 30 PPM C91252 126 145 151 146 144 136 120 137 148 147 C91253 142 143 130 138 130 108 125 139 141 138 C91254 117 124 121 115 117 107 112 125 129 121 C91255 128 139 136 131 128 135 130 118 123 128 C91256 142 152 142 130 128 139 136 132 143 157 C91257 131 133 124 128 126 128 125 127 109 110 C91258 140 155 151 136 125 131 141 134 145 142 C91259 147 164 161 134 127 68 71 C91260 152 166 164 158 154 156 161 136 58 85 C91261 136 149 153 150 144 148 144 140 132 141 C91262 118 132 114 124 125 123 125 123 142 144 C91263 160 167 167 161 157 155 153 87 159 NOT TAKEN C91264 123 142 132 134 127 126 123 122 125 125 C91265 136 129 119 143 138 119 145 136 127 139 C91266 177 181 164 162 151 154 161 156 173 178 C91267 126 144 133 139 138 145 170 163 C91268 129 135 131 131 137 119 132 125 139 170 C91269 143 186 165 167 165 178 158 161 180 178 C91270 147 167 156 90 140 99 C91271 149 191 173 158 160 164 183 168 198 165 C91273 144 151 159 152 141 145 136 146 159 155 C91274 127 142 138 132 139 150 141 143 132 C91275 150 166 178 153 145 130 104 SPILLED 145 C91276 141 149 134 136 128 127 131 122 131 130 C91277 134 136 129 123 SPILLED 126 133 132 141 NOT TAKEN C91278 141 145 134 125 125 123 123 132 131 131 C91279 209 213 201 192 190 197 SPILLED 195 250 212 C91280 145 156 141 135 145 148 149 144 153 152 C91282 161 201 173 90 10 C91283 147 150 155 138 144 148 147 129 142 156 C91284 143 139 150 108 123 129 118 125 155 142 1366 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 61 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 30 PPM C91286 159 164 139 C91289 131 164 131 C91290 187 195 184 C91292 126 153 142 C91294 123 141 139 C91295 115 132 140 C91296 118 141 132 C91297 180 SPILLED 151 C91298 142 213 175 C91300 148 170 152 C91301 110 148 141 C91302 149 158 155 C91303 135 141 152 C91305 177 185 187 C91306 146 158 156 C91307 138 146 130 C91308 SPILLED 208 191 C91309 158 171 163 C91310 129 153 151 129 138 SPILLED 132 128 123 106 80 87 128 169 161 67 142 143 158 161 145 136 103 128 123 121 145 137 128 137 140 123 121 116 113 118 151 142 153 162 136 164 SPILLED SPILLED SPILLED 118 146 135 140 106 89 141 148 145 139 146 159 140 68 157 143 144 145 147 134 162 173 184 168 162 174 143 134 150 151 136 143 131 141 145 137 180 167 151 132 125 161 156 154 154 131 154 144 141 142 136 150 131 84 111 163 165 140 162 135 155 122 134 139 143 118 140 94 98 167 163 90 NOT TAKEN 163 147 178 174 143 142 151 145 113 146 149 GROUP: FEMALE 4 - 100 PPM C91311 139 154 125 129 124 122 124 NOT TAKEN C91312 114 120 108 113 107 105 108 106 112 113 C91313 153 156 148 147 141 144 143 133 139 139 C91314 200 226 165 226 164 190 SPILLED SPILLED 204 114 C91315 131 145 138 142 141 137 136 138 135 134 C91319 148 155 143 137 134 136 133 123 117 C91320 141 146 137 144 145 147 142 143 150 140 C91321 144 170 158 155 159 154 158 145 169 157 C91323 167 183 173 166 150 1367 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 62 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91324 112 121 111 114 112 105 109 118 119 121 C91325 124 136 118 111 94 111 121 121 140 130 C91326 126 136 122 126 123 124 118 112 128 113 C91327 122 135 114 123 122 125 125 121 128 144 C91328 109 144 143 140 130 105 137 C91330 142 162 151 147 138 155 152 140 150 150 C91331 153 172 157 151 155 159 159 142 139 127 C91332 119 134 123 112 117 118 SPILLED 117 128 124 C91334 142 152 138 139 134 128 123 128 132 140 C91335 115 137 119 117 110 101 116 115 116 129 C91336 271 243 SPILLED SPILLED SPILLED SPILLED SPILLED SPILLED 205 SPILLED C91338 90 109 81 103 97 86 93 95 113 101 C91339 145 153 138 142 139 134 135 149 146 148 C91340 125 149 124 143 150 137 142 126 141 127 C91341 149 164 132 147 149 148 155 145 152 155 C91342 130 138 120 120 130 103 119 134 135 112 C91343 128 132 118 124 121 C91344 116 124 125 79 98 103 116 104 114 113 C91346 142 161 150 144 140 147 136 127 104 NOT TAKEN C91347 SPILLED 137 127 136 131 C91348 109 133 137 142 136 C91349 SPILLED SPILLED SPILLED SPILLED SPILLED 181 C91351 127 142 150 130 146 140 155 151 153 167 C91352 148 160 141 155 156 C91353 143 150 120 144 135 C91354 106 119 111 105 106 106 100 83 88 94 C91356 SPILLED 176 167 153 SPILLED 191 194 160 179 161 C91357 97 102 91 91 95 91 95 105 93 108 C91358 140 179 163 170 167 154 161 157 146 155 C91359 128 166 134 134 134 131 126 122 143 154 C91360 131 146 130 123 123 120 126 119 120 120 1368 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 63 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91361 112 119 119 122 117 125 127 124 116 114 C91362 135 156 146 135 149 128 138 147 158 164 C91363 151 138 132 132 130 127 136 135 134 140 C91364 113 129 111 116 113 110 115 103 112 117 C91365 SPILLED SPILLED 188 211 197 C91366 140 155 138 144 153 147 155 140 151 165 C91367 154 163 139 127 140 C91368 121 131 110 126 133 C91369 138 164 138 149 141 142 158 148 173 174 C91371 154 170 156 221 160 C91372 118 131 115 122 112 111 123 112 120 119 C91373 167 183 166 165 178 149 151 133 2 C91374 122 136 126 125 138 117 119 125 133 135 C91375 117 134 126 132 127 117 127 117 140 140 C91376 120 129 119 119 118 115 113 114 72 120 C91377 143 134 118 137 137 141 134 129 145 C91378 113 129 105 115 112 134 131 161 161 SPILLED C91380 124 143 134 137 127 131 127 119 121 129 GROUP: FEMALE 6 - 100 PPM C91451 152 140 126 121 126 145 129 120 143 152 C91452 146 160 153 143 142 154 172 155 166 138 C91453 143 163 145 135 133 148 145 125 164 169 C91454 121 127 116 112 108 114 137 120 113 121 C91455 125 134 121 124 121 128 126 117 129 132 C91456 117 138 122 119 117 140 141 139 147 143 C91457 134 149 136 130 127 144 145 138 154 156 C91458 127 142 126 137 125 142 128 C91459 SPILLED SPILLED 224 SPILLED SPILLED SPILLED SPILLED 184 171 174 C91460 106 120 100 102 90 90 69 1369 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 64 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 100 PPM C91461 142 169 157 161 156 179 188 183 188 184 C91462 140 162 151 140 139 149 143 138 161 158 C91463 129 136 118 122 126 134 149 137 145 155 C91464 121 136 119 126 131 137 140 126 128 84 C91465 145 169 154 146 142 176 166 152 161 182 C91466 128 155 129 137 143 136 148 159 178 163 C91467 131 150 140 142 139 145 144 140 155 168 C91468 SPILLED SPILLED 154 123 139 150 SPILLED C91469 123 134 119 119 123 131 135 125 93 NOT TAKEN C91470 156 180 150 165 151 150 161 128 0 C91471 136 157 138 143 138 146 152 133 147 153 C91472 122 139 126 135 123 135 143 145 129 157 C91473 116 147 108 107 118 121 135 120 136 143 C91474 147 172 128 139 136 136 SPILLED 146 166 161 C91475 161 171 162 159 147 159 194 167 176 170 C91476 144 158 140 144 136 142 162 157 152 189 C91477 145 155 141 147 135 143 149 156 162 182 C91478 149 166 159 167 156 170 SPILLED 155 173 180 C91479 147 177 154 140 147 174 167 153 157 171 C91480 134 146 139 139 127 129 134 126 139 141 C91481 110 128 124 130 127 136 157 131 172 113 C91482 138 152 130 140 132 141 155 148 162 162 C91483 136 147 129 131 92 132 145 138 141 144 C91484 127 127 133 123 123 108 120 74 43 C91485 121 134 122 113 92 134 133 122 137 155 C91486 136 141 133 138 137 152 177 SPILLED 176 157 C91487 125 144 123 136 130 159 161 163 180 189 C91488 133 139 132 135 121 132 149 142 158 178 C91489 141 163 142 149 143 154 177 165 170 192 C91490 SPILLED 152 137 141 138 144 160 136 133 201 1370 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 65 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C90711 38 C90712 184 183 182 167 162 158 116 SPILLED C90713 211 211 199 154 129 107 C90715 212 196 181 126 109 C90716 195 193 203 193 164 162 171 166 C90720 200 196 179 152 C90722 189 188 195 183 204 192 208 184 C90724 200 209 216 199 189 208 193 191 C90726 134 180 168 158 CNTMD SPILLED SPILLED SPILLED C90727 204 211 200 188 130 38 C90728 198 185 186 195 189 176 160 155 C90729 184 191 187 179 NOT TAKEN C90733 65 216 SPILLED 191 SPILLED C90734 170 NOT TAKEN C90735 190 198 199 178 60 191 204 SPILLED C90738 159 183 172 181 182 151 C90739 190 175 199 171 199 186 50 C90741 194 186 177 192 125 37 NOT TAKEN C90742 192 179 175 168 171 162 150 155 C90743 222 234 209 201 198 189 182 186 C90744 223 198 163 C90745 25 C90747 167 171 SPILLED 194 181 155 170 175 C90749 180 179 170 181 24 C90750 191 190 183 170 176 156 171 160 C90755 241 241 229 218 194 181 NOT TAKEN C90758 203 SPILLED 235 199 170 157 163 SPILLED C90759 163 161 71 C90760 154 118 SPILLED C90761 185 194 190 176 169 C90762 167 C90763 184 202 136 1371 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 66 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C90764 177 C90765 228 C90770 197 C90771 190 C90776 234 C90777 205 C90778 182 C90779 177 181 NOT TAKEN 226 227 186 224 190 204 203 195 122 51 NOT TAKEN 205 169 68 103 226 230 230 239 200 NOT TAKEN 210 173 NOT TAKEN 175 190 174 189 180 183 193 190 173 121 3 GROUP: MALE 2 - 3 PPM C90781 C90782 C90783 C90784 C90785 C90786 C90787 C90788 C90790 C90791 C90793 C90794 C90798 C90799 C90800 C90801 C90802 C90803 C90804 C90809 195 192 193 187 183 180 188 154 195 171 SPILLED 164 174 132 188 183 172 176 85 162 160 145 145 138 164 167 161 131 87 190 189 222 206 219 193 136 32 171 188 188 165 160 213 217 207 199 181 200 131 145 199 196 221 190 197 185 189 186 140 61 190 199 197 186 193 190 176 172 131 138 SPILLED SPILLED SPILLED 224 SPILLED 186 SPILLED 233 240 231 182 183 193 38 183 177 167 150 161 155 158 148 201 198 179 SPILLED 236 218 228 217 139 3 116 191 287 280 SPILLED 194 206 187 172 162 137 96 66 157 SPILLED SPILLED SPILLED SPILLED 227 195 SPILLED 1372 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 67 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 3 PPM C90810 C90811 C90812 C90815 C90816 C90819 C90821 C90822 C90823 C90824 C90825 C90826 C90828 C90829 C90830 C90832 C90835 C90838 C90839 C90840 SPILLED 201 195 188 179 211 222 0 175 196 209 239 183 249 192 206 208 237 203 197 225 203 193 181 189 239 226 179 24 206 233 NOT TAKEN 232 189 167 214 235 166 SPILLED 222 196 198 173 43 239 229 194 217 253 245 211 221 182 240 229 SPILLED 204 178 185 156 208 219 164 201 211 223 175 187 197 223 SPILLED SPILLED 199 190 173 164 NOT TAKEN 225 137 135 NOT TAKEN 216 186 160 196 202 138 SPILLED 205 175 139 138 216 157 192 216 162 191 176 213 159 SPILLED 191 175 182 184 112 97 106 120 NOT TAKEN 163 178 248 176 199 159 207 SPILLED 167 150 190 160 188 SPILLED GROUP: MALE 3 - 30 PPM C90841 201 209 189 149 116 41 C90842 147 155 157 125 128 122 129 137 C90844 235 226 228 210 208 197 161 120 C90845 119 C90847 199 201 149 137 112 31 C90848 NOT TAKEN C90849 81 C90850 183 194 184 183 143 98 142 116 1373 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 68 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90851 190 185 184 163 173 184 179 170 C90854 208 204 199 177 169 162 163 112 C90855 177 193 182 160 168 157 160 165 C90856 189 198 209 187 193 180 162 180 C90857 179 184 203 180 176 137 87 C90858 160 186 162 148 127 SPILLED 168 117 C90860 176 169 177 154 150 152 151 159 C90864 164 178 179 160 160 150 165 172 C90865 173 170 173 166 165 150 153 144 C90867 183 196 181 175 169 159 168 172 C90869 217 217 224 211 196 177 164 191 C90870 117 NOT TAKEN C90871 149 181 170 160 163 144 156 156 C90872 200 201 214 136 C90875 196 196 202 191 C90879 123 37 C90881 225 225 217 214 218 201 212 199 C90882 208 226 226 208 220 204 194 196 C90883 185 179 225 175 165 189 144 161 C90884 210 SPILLED 193 176 202 177 168 84 C90885 192 176 184 151 194 SPILLED 200 148 C90886 231 172 98 92 C90888 159 1 C90889 190 171 197 178 192 176 165 168 C90892 1 C90893 203 204 197 189 200 199 196 195 C90894 191 180 194 162 159 147 C90895 182 190 190 174 165 C90897 215 208 218 203 211 C90899 186 179 180 189 177 160 97 NOT TAKEN C90900 179 183 173 162 170 161 148 156 1374 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 69 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90903 C90904 C90906 C90908 C90910 C90911 C90912 C90914 C90915 C90916 C90919 C90920 C90923 C90925 C90926 C90927 C90928 C90930 C90931 C90933 C90934 C90936 C90937 C90941 C90943 C90944 C90946 C90947 C90948 C90953 C90955 166 168 179 166 168 164 176 196 191 191 187 205 169 180 186 173 190 185 121 90 213 222 212 221 220 212 191 188 181 166 214 217 209 215 162 56 207 202 188 208 207 210 187 191 186 168 180 35 169 184 171 193 192 157 184 165 167 192 SPILLED 192 205 SPILLED 206 196 190 198 180 109 81 146 SPILLED 177 157 165 165 78 190 169 181 183 195 174 181 177 177 182 207 204 156 156 152 115 151 136 136 142 123 198 205 197 108 177 173 SPILLED 180 167 160 178 126 183 180 168 159 155 169 168 165 138 91 NOT TAKEN 207 195 158 168 156 156 158 175 214 218 178 NOT TAKEN 164 26 162 197 103 SPILLED SPILLED 178 148 160 176 174 64 164 123 126 173 165 173 174 129 199 171 199 183 163 186 171 167 123 188 208 185 126 170 151 140 120 85 NOT TAKEN 159 146 147 161 148 186 177 182 181 176 151 158 SPILLED 154 SPILLED 162 171 154 161 162 183 187 160 164 165 1375 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 70 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90956 204 218 205 193 181 166 160 SPILLED C90958 55 163 SPILLED 173 139 C90959 187 207 136 C90965 SPILLED 190 SPILLED 181 176 158 175 176 C90968 193 SPILLED 83 161 107 111 SPILLED 151 C90969 218 210 150 C90970 180 185 193 159 177 187 NOT TAKEN GROUP: MALE 6 - 100 PPM C91042 201 205 218 196 99 C91043 184 193 200 C91047 176 165 185 148 116 173 158 164 C91048 116 NOT TAKEN C91050 225 217 219 199 143 164 160 C91051 217 212 113 2 C91053 186 192 C91054 210 226 230 228 214 186 220 176 C91055 239 222 C91056 196 201 184 184 171 163 142 149 C91058 172 187 C91059 173 31 C91060 202 204 212 190 169 159 167 173 C91061 188 193 192 129 C91062 177 52 102 42 155 C91063 150 152 159 148 80 45 NOT TAKEN C91064 190 193 205 181 162 192 C91065 168 157 143 169 159 173 197 161 C91066 127 173 154 C91067 189 209 209 206 193 163 NOT TAKEN C91069 239 252 NOT TAKEN 1376 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 71 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 100 PPM C91070 164 C91071 221 C91072 164 C91073 228 C91074 195 C91075 112 C91076 213 C91077 194 C91078 184 C91079 186 C91080 185 176 172 163 148 141 146 134 225 223 221 214 214 199 193 159 140 119 113 129 123 SPILLED 216 177 213 SPILLED 185 SPILLED SPILLED 173 206 196 SPILLED SPILLED SPILLED 224 173 222 168 NOT TAKEN 197 190 205 141 75 192 201 197 218 SPILLED SPILLED 171 188 171 105 GROUP: FEMALE 1 - 0 PPM C91123 170 146 171 159 174 SPILLED C91125 154 160 167 162 104 NOT TAKEN C91127 157 159 158 175 153 160 113 7 C91130 56 C91131 165 157 156 143 127 130 115 125 C91132 136 142 162 121 C91136 175 168 SPILLED 63 C91137 150 151 147 117 142 103 68 C91138 149 164 165 138 136 139 152 144 C91141 177 NOT TAKEN C91144 108 126 131 114 110 108 107 102 C91147 61 C91148 155 171 195 157 172 171 149 144 C91149 168 SPILLED 144 NOT TAKEN C91150 NOT TAKEN C91153 206 189 C91154 131 1377 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 72 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C91157 C91158 C91159 C91164 C91166 C91167 C91168 C91170 C91172 C91175 C91176 C91177 C91178 C91182 C91183 C91184 C91186 C91188 C91189 C91190 134 130 135 130 119 112 112 126 193 193 181 163 161 146 169 151 153 152 138 134 152 196 196 154 150 87 143 174 149 152 145 146 144 142 208 153 SPILLED 149 SPILLED 152 147 182 185 NOT TAKEN 118 150 78 SPILLED 246 SPILLED 213 176 SPILLED 225 153 194 186 192 121 169 196 199 192 176 94 SPILLED 72 180 179 179 168 141 172 164 158 95 185 77 5 NOT TAKEN 197 205 207 200 195 185 176 193 206 198 166 46 163 177 166 147 89 145 156 145 165 158 128 133 139 143 124 129 144 126 65 153 174 157 166 164 169 151 120 GROUP: FEMALE 2 - 3 PPM C91191 176 177 173 170 205 99 C91193 119 SPILLED 138 62 C91194 178 SPILLED 163 135 135 115 53 NOT TAKEN C91195 166 169 180 156 104 55 NOT TAKEN C91196 150 22 C91197 144 164 164 162 166 153 143 154 C91199 147 177 155 163 88 106 C91200 148 155 149 1378 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 73 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91201 164 166 151 161 152 103 65 126 C91202 185 189 121 C91203 NOT TAKEN C91207 141 158 146 135 47 149 136 147 C91211 152 168 160 162 177 160 151 140 C91213 117 C91214 181 180 140 165 188 135 C91216 159 145 SPILLED SPILLED 130 142 147 150 C91218 173 144 63 96 129 SPILLED C91219 179 146 63 NOT TAKEN C91220 174 178 164 134 177 170 149 129 C91221 163 166 164 167 169 176 153 134 C91224 131 137 149 138 97 99 C91225 140 55 NOT TAKEN C91226 112 85 176 C91227 157 145 137 117 126 132 132 145 C91228 194 SPILLED 194 107 78 C91229 80 163 101 101 26 C91231 166 179 184 134 NOT TAKEN C91232 140 153 148 153 151 133 143 121 C91235 SPILLED SPILLED SPILLED 130 136 138 126 132 C91236 152 169 169 131 145 141 139 123 C91240 148 C91242 SPILLED SPILLED SPILLED 141 134 137 129 137 C91243 178 181 193 165 157 158 129 128 C91245 175 169 174 164 101 NOT TAKEN C91246 133 161 117 C91249 195 191 181 155 21 22 1379 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 74 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 30 PPM C91252 149 164 157 152 164 143 C91253 144 157 162 148 126 132 C91254 122 118 130 124 107 134 C91256 SPILLED 170 178 158 150 110 C91257 102 89 C91258 123 168 11 C91261 135 146 130 113 114 86 C91262 133 137 122 1 C91264 127 159 149 138 136 129 C91265 163 148 152 119 42 C91266 175 159 169 155 144 129 C91268 143 168 143 165 160 140 C91269 63 48 C91271 192 143 98 121 137 C91273 160 104 C91276 124 143 136 130 131 153 C91278 140 135 131 120 132 131 C91279 200 SPILLED SPILLED 205 SPILLED 157 C91280 162 162 157 155 158 72 C91283 151 183 148 2 SPILLED C91284 143 155 150 135 143 130 C91286 136 138 142 116 127 124 C91292 171 172 186 165 176 174 C91294 175 171 169 SPILLED 167 166 C91295 151 177 157 145 171 151 C91296 125 76 63 C91297 136 150 140 163 C91300 119 SPILLED C91301 151 162 182 173 153 148 C91303 164 165 153 129 158 135 C91305 175 159 155 137 142 129 136 121 139 109 125 119 82 55 NOT TAKEN 107 115 162 155 124 120 166 176 156 135 136 80 134 151 121 130 1380 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 75 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 30 PPM C91306 147 141 141 150 129 120 137 C91307 164 162 147 154 139 125 101 67 C91310 160 170 178 167 142 153 154 157 GROUP: FEMALE 4 - 100 PPM C91312 115 116 109 100 87 19 C91313 128 133 82 C91315 149 163 SPILLED SPILLED 150 148 149 145 C91320 106 C91321 162 168 154 111 103 C91324 132 134 129 98 120 109 116 108 C91325 130 152 135 136 128 105 54 C91326 105 84 86 C91327 140 144 157 135 173 161 150 141 C91330 171 130 61 C91331 38 C91332 197 SPILLED 150 114 118 136 94 106 C91334 143 147 132 130 107 101 152 C91335 125 136 134 126 127 114 101 113 C91336 SPILLED SPILLED SPILLED SPILLED 225 SPILLED 207 SPILLED C91338 110 107 122 116 104 96 107 88 C91339 160 176 160 141 84 102 C91340 140 155 139 133 135 124 83 150 C91341 160 148 167 166 155 170 148 174 C91342 136 133 145 114 131 133 125 125 C91344 123 140 118 116 104 69 91 C91351 157 161 127 124 55 C91354 99 95 97 NOT TAKEN C91356 205 228 201 172 158 147 157 79 C91357 105 102 101 96 91 101 95 92 1381 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 76 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91359 150 C91360 129 141 133 112 123 121 116 116 C91361 128 125 118 105 115 106 109 98 C91362 163 164 185 176 161 162 153 144 C91363 140 123 154 138 132 SPILLED C91364 108 118 97 85 102 95 140 C91366 147 147 128 C91372 123 134 137 112 116 103 110 103 C91374 150 152 150 122 75 31 C91375 153 81 C91376 113 126 125 111 114 112 119 110 C91378 158 SPILLED SPILLED 168 189 SPILLED 181 128 C91380 125 122 150 121 139 182 178 205 GROUP: FEMALE 6 - 100 PPM C91451 148 165 157 158 146 161 142 161 C91453 148 168 154 193 140 218 241 204 C91454 149 99 C91455 132 132 143 132 108 120 113 126 C91456 2 C91457 161 167 188 173 167 NOT TAKEN C91459 186 159 188 176 163 168 117 144 C91461 185 219 98 C91462 164 154 151 142 134 135 132 124 C91463 146 153 138 C91465 153 99 69 SPILLED C91466 168 197 164 156 2 C91467 166 139 162 179 135 165 153 129 C91471 156 157 152 147 139 61 C91472 141 170 164 167 125 SPILLED 1382 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 77 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 100 PPM C91473 C91474 C91475 C91476 C91477 C91478 C91479 C91480 C91482 C91483 C91485 C91486 C91487 C91488 C91489 C91490 139 169 168 140 149 154 141 144 175 170 114 79 98 195 186 156 177 178 146 NOT TAKEN 99 NOT TAKEN 198 109 93 174 161 151 174 171 170 181 162 177 155 173 149 155 163 142 155 142 110 146 131 91 93 77 79 151 141 132 148 119 151 154 160 142 149 151 117 120 129 75 175 SPILLED NOT TAKEN 176 197 173 195 162 174 166 174 151 159 148 150 140 147 143 178 174 183 160 153 1383 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 3 PPM C90781 C90782 C90783 C90784 C90785 C90786 C90787 C90788 C90789 C90790 C90791 C90792 C90793 C90794 C90795 C90796 C90797 C90798 C90799 C90800 C90801 C90802 C90803 C90804 C90805 C90806 C90807 C90808 C90809 C90810 C90811 0.231 SPILLED 0.256 0.250 0.251 0.263 0.253 0.326 0.246 0.263 0.257 0.260 0.255 0.274 0.244 0.249 0.246 0.273 0.272 0.241 0.248 0.253 0.274 0.247 0.276 0.241 0.239 0.248 0.276 0.253 0.260 0.213 0.224 0.225 0.224 0.221 0.225 0.224 0.231 0.222 0.232 0.239 0.237 0.229 0.258 0.223 0.227 0.217 0.242 0.235 0.224 0.225 0.241 0.247 0.229 0.245 0.216 0.217 0.226 0.242 0.238 0.223 0.192 0.218 0.212 0.208 0.204 0.194 0.207 0.214 0.205 0.215 0.211 0.216 0.219 0.228 0.200 0.231 0.200 0.221 0.212 SPILLED 0.211 0.210 0.213 0.207 0.215 0.193 0.199 0.210 0.220 0.213 0.201 0.180 0.207 0.202 0.194 0.194 0.192 0.197 0.197 NOT TAKEN 0.197 0.207 0.214 0.201 0.211 0.193 0.209 0.185 0.145 0.195 0.186 0.203 0.203 0.199 0.192 0.209 0.197 0.188 0.196 0.208 0.197 0.196 0.171 SPILLED 0.190 0.181 0.181 0.181 0.188 0.182 NOT TAKEN 0.191 0.203 0.200 0.186 0.210 0.191 0.199 0.176 0.187 0.178 0.174 0.198 0.184 0.188 0.179 0.198 0.189 0.174 0.190 SPILLED 0.186 0.203 0.167 0.193 0.177 0.175 0.178 0.169 0.184 0.168 NOT TAKEN 0.179 0.186 0.183 0.173 0.187 0.176 0.187 0.167 0.169 0.170 0.169 0.186 0.176 0.174 0.174 0.188 0.177 0.159 0.175 0.180 0.174 0.185 0.159 0.221 0.163 0.162 0.173 0.164 0.179 0.162 NOT TAKEN 0.177 0.180 0.170 0.171 0.178 0.179 0.194 0.167 0.180 0.172 0.160 0.183 0.168 0.159 0.170 0.174 0.170 0.157 0.169 0.182 0.169 0.183 SPILLED 0.171 0.164 0.159 0.170 0.158 0.170 0.155 NOT TAKEN 0.170 0.172 0.165 0.163 0.172 0.176 0.189 0.157 0.155 0.164 0.157 0.179 0.163 0.155 0.156 0.167 0.165 0.152 0.164 0.164 0.156 0.170 0.153 0.181 0.153 0.151 0.158 0.140 0.161 0.147 NOT TAKEN 0.157 0.167 0.159 0.155 0.164 0.165 0.188 0.145 0.167 0.152 0.143 0.170 0.148 0.145 0.150 0.157 0.158 0.142 0.156 0.158 0.149 0.160 0.150 0.163 0.148 0.149 0.156 0.140 0.156 0.142 NOT TAKEN 0.154 0.159 0.147 0.146 0.156 0.160 0.182 0.143 0.146 0.146 0.139 0.167 0.146 0.139 0.144 0.155 0.155 0.144 0.150 0.164 0.144 0.160 1384 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 3 PPM C90812 C90813 C90814 C90815 C90816 C90817 C90818 C90819 C90820 C90821 C90822 C90823 C90824 C90825 C90826 C90827 C90828 C90829 C90830 C90831 C90832 C90833 C90834 C90835 C90836 C90837 C90838 C90839 C90840 0.249 0.249 0.242 0.260 0.239 0.247 0.262 0.256 0.244 0.259 0.246 0.255 0.296 0.255 0.306 0.257 0.275 0.274 0.261 0.250 0.268 0.272 0.257 0.251 0.266 0.274 0.251 0.249 0.266 0.221 0.220 0.221 0.237 0.208 0.215 0.251 0.231 0.211 0.224 0.227 0.225 0.248 0.236 SPILLED 0.236 0.255 0.248 0.240 0.232 0.244 0.257 0.234 0.228 0.223 0.240 0.223 0.223 0.239 0.199 0.203 0.205 0.215 0.186 0.196 0.216 0.211 0.201 0.201 0.210 0.203 0.230 0.225 SPILLED 0.215 0.231 0.216 0.221 0.207 0.222 0.247 0.208 0.202 0.199 0.221 0.198 0.203 0.216 0.185 0.189 NOT TAKEN 0.197 0.188 NOT TAKEN 0.207 0.188 0.185 0.191 0.195 0.198 0.199 0.204 SPILLED 0.198 0.221 0.200 0.203 0.196 0.210 NOT TAKEN NOT TAKEN 0.196 0.202 0.210 0.189 0.194 0.201 0.169 0.184 NOT TAKEN 0.186 0.174 NOT TAKEN 0.200 0.177 0.178 0.175 0.181 0.184 0.181 0.190 SPILLED 0.187 0.199 0.185 0.196 0.185 0.190 NOT TAKEN NOT TAKEN 0.190 0.193 0.194 0.181 0.184 0.191 0.163 0.175 NOT TAKEN 0.177 0.167 NOT TAKEN 0.194 0.175 0.164 0.171 0.173 0.174 0.182 0.179 SPILLED 0.175 0.195 0.172 0.176 0.175 0.177 NOT TAKEN NOT TAKEN 0.177 0.182 0.190 0.170 0.175 0.178 0.160 0.169 NOT TAKEN 0.174 0.166 NOT TAKEN SPILLED 0.164 0.162 0.162 0.174 0.177 0.173 0.170 SPILLED 0.170 0.181 0.171 0.166 0.175 0.177 NOT TAKEN NOT TAKEN 0.195 0.177 0.178 0.166 0.166 0.174 0.154 NOT TAKEN NOT TAKEN 0.165 0.156 NOT TAKEN 0.178 0.157 0.151 0.154 0.163 0.171 0.166 0.159 SPILLED 0.158 0.182 0.161 0.164 0.173 0.162 NOT TAKEN NOT TAKEN 0.171 0.168 0.169 0.155 0.163 0.166 0.149 NOT TAKEN NOT TAKEN 0.157 0.156 NOT TAKEN SPILLED 0.146 0.146 0.144 0.156 0.165 0.151 0.155 SPILLED 0.152 0.175 0.153 0.155 0.163 0.151 NOT TAKEN NOT TAKEN 0.171 0.158 0.158 0.153 0.153 0.157 0.137 NOT TAKEN NOT TAKEN 0.154 0.152 NOT TAKEN 0.181 0.140 0.143 0.140 0.151 0.165 0.144 0.150 0.191 0.144 0.171 0.142 0.148 0.151 0.146 NOT TAKEN NOT TAKEN 0.155 0.155 0.150 0.147 0.144 0.148 1385 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90841 C90842 C90843 C90844 C90845 C90846 C90847 C90848 C90849 C90850 C90851 C90852 C90853 C90854 C90855 C90856 C90857 C90858 C90859 C90860 C90861 C90862 C90863 C90864 C90865 C90866 C90867 C90868 C90869 C90870 C90871 2.705 2.495 2.656 2.623 SPILLED 2.343 2.519 2.552 2.530 2.431 2.546 2.582 SPILLED 2.733 3.093 2.579 2.470 2.636 2.635 2.745 2.372 2.883 2.911 2.656 2.543 2.420 2.489 2.523 2.877 2.669 2.423 2.367 2.481 2.401 2.401 2.168 2.088 2.270 2.334 2.235 2.171 2.418 2.582 2.213 2.525 2.502 2.328 2.203 2.383 2.405 2.439 2.113 2.720 2.552 2.351 2.188 1.983 2.201 2.101 2.480 SPILLED 2.143 2.123 1.941 2.077 2.157 2.007 1.855 2.045 2.149 2.015 2.052 2.050 2.108 2.193 2.259 2.188 1.994 1.910 2.095 2.236 2.182 2.016 2.396 2.246 2.110 2.059 1.819 2.026 1.962 2.253 SPILLED 1.923 2.032 1.963 2.064 SPILLED 1.887 NOT TAKEN 1.908 2.057 1.833 1.909 1.993 1.929 NOT TAKEN 2.089 1.950 1.929 1.914 2.022 2.002 2.018 1.912 2.418 SPILLED 1.917 1.912 NOT TAKEN 1.849 1.873 2.042 SPILLED 1.841 2.032 1.851 1.813 1.967 1.822 NOT TAKEN 1.744 1.912 1.712 1.827 1.992 1.824 NOT TAKEN SPILLED 1.884 1.746 1.787 1.831 1.837 1.964 1.782 2.076 1.868 1.791 1.716 NOT TAKEN 1.779 1.776 1.885 1.889 1.755 1.952 1.709 1.793 SPILLED 1.741 NOT TAKEN 1.698 1.796 1.665 1.729 1.871 1.766 NOT TAKEN SPILLED 1.667 1.664 1.718 1.793 1.734 SPILLED 1.724 2.037 1.685 1.769 1.666 NOT TAKEN 1.690 1.704 1.700 1.781 1.644 1.852 1.705 SPILLED 1.819 1.670 NOT TAKEN 1.594 1.755 1.531 1.710 1.794 1.677 NOT TAKEN SPILLED 1.590 1.696 1.687 1.781 1.725 1.801 1.660 1.850 1.611 1.769 1.581 NOT TAKEN 1.685 1.613 1.655 1.680 1.551 1.733 1.559 1.670 SPILLED 1.610 NOT TAKEN 1.527 1.675 1.485 1.580 1.644 1.721 NOT TAKEN SPILLED 1.550 1.543 1.554 1.685 1.653 1.788 1.643 1.824 1.543 1.722 1.547 NOT TAKEN 1.568 1.568 1.582 1.603 1.424 1.655 1.590 1.631 1.805 1.515 NOT TAKEN 1.538 1.657 1.459 1.531 SPILLED 1.547 NOT TAKEN SPILLED 1.461 1.506 1.534 1.485 1.606 1.583 1.519 1.726 1.453 1.607 1.462 NOT TAKEN 1.573 1.507 1.510 1.531 1.384 1.579 1.477 1.520 1.690 1.451 NOT TAKEN 1.424 1.515 1.500 1.442 1.532 1.532 NOT TAKEN 1.606 1.427 1.511 1.490 1.492 1.572 1.593 1.493 1.749 1.369 1.524 1.467 NOT TAKEN 1.483 1.446 1.395 1.520 1.371 1386 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90872 C90873 C90874 C90875 C90876 C90877 C90878 C90879 C90880 C90881 C90882 C90883 C90884 C90885 C90886 C90887 C90888 C90889 C90890 C90891 C90892 C90893 C90894 C90895 C90896 C90897 C90898 C90899 C90900 2.263 2.380 2.447 2.639 2.715 2.451 2.609 2.731 2.672 2.637 2.551 2.699 2.777 2.743 2.751 2.522 2.378 2.628 2.654 2.646 2.487 2.933 2.704 3.101 2.818 2.531 2.540 2.615 2.606 SPILLED 2.087 2.143 2.307 2.287 2.160 2.361 2.234 2.336 2.332 2.223 2.577 2.399 2.327 2.522 2.219 2.102 2.243 2.335 2.410 2.169 2.543 2.327 2.843 SPILLED 2.280 2.267 2.358 2.253 1.905 1.909 1.949 2.137 2.106 2.018 2.153 2.152 2.161 2.126 2.042 2.171 2.244 2.101 2.115 2.026 1.944 2.033 2.109 2.129 2.058 2.310 2.055 2.453 SPILLED 2.062 2.025 2.113 2.070 1.836 1.881 1.837 1.934 1.826 1.942 2.165 2.000 1.903 1.984 1.924 SPILLED 1.926 1.851 2.060 1.865 1.858 1.761 NOT TAKEN NOT TAKEN 1.964 2.114 1.981 2.094 SPILLED 1.917 1.876 1.949 1.919 1.742 1.817 1.694 1.860 1.680 1.846 2.033 1.799 1.817 1.833 1.855 2.138 1.812 1.731 2.028 1.794 1.730 1.697 NOT TAKEN NOT TAKEN 1.943 2.012 1.918 2.233 1.854 1.854 1.820 1.849 1.778 1.710 1.735 1.604 1.724 1.525 1.692 1.916 1.674 1.714 1.661 1.788 1.999 1.742 1.589 1.835 1.653 1.585 1.610 NOT TAKEN NOT TAKEN 1.789 1.888 1.793 1.878 1.777 1.694 1.673 1.725 1.656 1.622 1.653 1.520 1.634 1.574 1.658 1.852 1.701 1.679 1.635 1.709 SPILLED 1.714 1.616 1.769 1.646 1.598 1.591 NOT TAKEN NOT TAKEN 1.783 1.850 1.796 1.872 SPILLED 1.686 1.726 1.816 1.661 1.547 1.526 1.489 1.594 1.517 1.608 1.833 1.648 1.626 1.535 1.649 1.863 1.686 1.542 1.731 1.634 1.534 1.560 NOT TAKEN NOT TAKEN 1.747 1.785 1.647 1.828 1.581 1.585 1.639 1.731 1.690 1.497 1.576 1.438 1.606 1.542 1.380 1.710 1.613 1.584 1.535 1.589 1.798 1.584 1.433 1.637 1.544 1.470 1.408 NOT TAKEN NOT TAKEN 1.676 1.652 1.577 1.897 SPILLED 1.500 1.507 1.640 1.570 1.406 1.506 1.396 1.447 1.452 1.560 1.634 1.548 1.478 1.456 1.532 1.810 1.641 1.412 1.525 1.517 1.431 1.358 NOT TAKEN NOT TAKEN 1.612 1.573 1.490 1.709 1.864 1.398 1.482 1.509 1.492 1387 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90901 C90902 C90903 C90904 C90905 C90906 C90907 C90908 C90909 C90910 C90911 C90912 C90913 C90914 C90915 C90916 C90917 C90918 C90919 C90920 C90921 C90922 C90923 C90924 C90925 C90926 C90927 C90928 C90929 C90930 C90931 8.445 8.946 8.627 8.516 8.621 8.318 7.838 8.440 8.524 8.988 8.993 7.651 9.250 8.937 8.666 8.131 8.815 7.768 9.171 8.431 8.561 8.280 8.098 8.845 8.640 8.212 9.193 7.975 8.733 9.311 7.917 7.312 7.534 7.402 7.154 SPILLED 7.085 6.744 7.250 7.684 7.583 7.562 6.683 8.170 7.597 7.578 7.343 7.625 7.025 7.776 7.441 7.568 6.961 7.027 7.758 SPILLED 7.664 8.157 7.073 8.026 7.726 6.756 6.442 6.531 6.673 6.671 7.098 6.568 6.345 6.851 6.843 7.088 6.865 6.303 7.030 6.865 7.220 6.749 7.153 6.377 7.407 6.842 6.665 6.672 6.593 7.353 6.936 7.048 7.213 6.597 6.868 6.946 6.385 6.282 6.429 6.409 6.362 6.877 6.434 6.076 6.445 6.375 6.618 6.345 5.991 6.524 6.425 6.712 6.197 6.373 5.982 6.704 6.552 5.970 5.965 6.045 6.623 6.245 6.412 6.740 6.125 6.577 6.452 6.424 5.987 5.974 6.078 5.778 6.495 6.186 5.844 6.010 5.727 6.362 6.133 5.727 6.030 6.040 6.458 5.949 6.062 5.727 6.379 6.021 5.974 5.729 5.680 6.345 5.848 6.083 6.308 5.685 6.274 5.474 6.108 5.815 5.601 5.847 5.495 6.003 5.701 5.434 5.644 5.404 6.087 5.694 5.330 5.216 5.533 5.917 5.505 5.577 5.380 5.796 5.619 5.542 5.419 5.445 5.842 5.630 5.634 5.857 5.370 5.896 5.932 5.863 5.427 5.226 5.417 5.442 5.757 5.491 5.032 5.273 5.503 5.821 5.323 5.211 3.090 5.413 5.363 5.116 5.383 5.278 5.541 5.550 5.330 5.113 5.232 5.880 5.316 5.422 5.601 5.216 5.575 5.766 5.377 5.120 5.174 5.089 5.104 5.714 5.183 4.837 5.068 5.378 5.663 5.088 4.800 4.920 5.073 5.396 5.189 5.261 5.200 5.413 5.292 5.145 5.133 5.276 5.623 5.384 5.273 5.306 4.966 5.679 5.602 5.142 4.818 4.713 4.924 4.909 5.465 5.170 4.704 4.834 4.872 5.229 5.146 4.881 6.099 4.934 4.946 4.896 4.900 4.803 5.259 5.181 4.825 4.590 4.962 5.106 4.976 4.867 4.963 4.673 5.480 5.458 4.928 4.959 4.783 4.607 4.839 5.374 4.879 4.721 4.876 4.857 5.181 4.970 4.591 5.690 4.833 4.951 4.801 4.498 4.550 4.899 4.890 4.790 4.620 5.056 5.161 4.989 4.641 4.666 4.587 5.338 5.396 4.629 1388 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90932 C90933 C90934 C90935 C90936 C90937 C90938 C90939 C90940 C90941 C90942 C90943 C90944 C90945 C90946 C90947 C90948 C90949 C90950 C90951 C90952 C90953 C90954 C90955 C90956 C90957 C90958 C90959 C90960 C90961 C90962 8.973 9.104 8.719 8.553 9.524 9.069 9.305 8.858 9.250 9.975 8.775 SPILLED 8.291 8.292 8.392 8.220 10.399 10.628 8.780 8.129 9.089 8.148 9.563 9.058 8.418 8.783 8.625 8.603 9.006 8.911 9.060 7.469 7.807 7.526 7.328 7.897 9.570 8.163 7.501 7.391 7.613 7.681 8.011 7.314 7.342 6.955 7.199 7.687 8.039 7.559 7.143 7.828 7.131 8.106 7.777 7.099 7.635 7.100 7.499 8.770 7.719 7.807 7.097 7.171 6.774 6.585 7.399 6.939 7.477 6.947 6.945 7.055 6.849 SPILLED 6.812 6.633 SPILLED 6.672 6.901 6.997 6.883 6.379 7.204 6.653 7.556 7.152 6.545 6.959 6.534 6.918 7.112 SPILLED 6.937 6.497 6.806 6.192 NOT TAKEN 6.137 6.634 7.015 NOT TAKEN 6.510 SPILLED NOT TAKEN SPILLED 6.213 6.208 6.122 6.231 6.530 6.288 6.094 5.403 6.427 6.378 6.565 6.410 6.091 6.649 6.313 6.198 6.669 NOT TAKEN 6.620 5.838 6.281 6.131 NOT TAKEN 6.408 5.570 6.443 NOT TAKEN 6.046 6.312 NOT TAKEN 7.161 5.690 5.812 5.907 5.858 6.286 6.622 5.708 5.742 5.762 5.756 6.379 5.961 5.809 6.299 6.135 5.978 6.369 NOT TAKEN 6.204 5.934 5.935 5.743 NOT TAKEN 6.143 6.353 6.128 NOT TAKEN 5.691 5.770 NOT TAKEN SPILLED 5.487 5.427 5.488 5.653 6.008 5.571 5.605 5.635 5.341 5.495 5.934 5.322 5.531 5.919 5.676 5.701 5.907 NOT TAKEN 5.825 5.760 5.702 5.353 NOT TAKEN 5.905 5.628 6.028 NOT TAKEN 5.515 5.672 NOT TAKEN 6.195 5.369 5.333 5.138 5.351 5.814 5.155 5.517 5.510 5.220 5.337 5.919 5.252 5.425 4.571 5.155 5.548 6.218 NOT TAKEN 5.460 5.612 5.503 5.201 NOT TAKEN 5.760 5.177 5.874 NOT TAKEN 5.478 5.348 NOT TAKEN 5.740 5.406 5.177 5.008 5.071 5.708 5.874 5.389 5.438 5.128 5.228 5.809 5.127 5.308 5.523 5.198 5.420 5.480 NOT TAKEN 5.418 5.233 5.019 5.642 NOT TAKEN 5.369 SPILLED 5.514 NOT TAKEN 5.147 4.901 NOT TAKEN 5.290 5.145 4.875 4.492 5.044 5.328 5.273 4.786 4.887 4.637 4.887 5.180 5.005 4.800 5.423 4.613 4.885 4.991 NOT TAKEN 4.981 5.050 4.969 5.329 NOT TAKEN 5.234 5.260 5.207 NOT TAKEN 4.766 4.950 NOT TAKEN 5.862 4.823 4.543 4.674 4.970 4.997 4.351 4.743 4.870 4.467 4.620 4.848 4.934 3.184 5.014 4.513 4.878 4.898 NOT TAKEN 4.876 1389 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90963 C90964 C90965 C90966 C90967 C90968 C90969 C90970 8.353 9.111 9.543 8.157 8.070 8.350 8.542 8.484 7.164 7.924 8.321 7.359 7.226 7.389 7.477 7.215 6.681 7.117 7.675 6.637 6.505 6.604 6.611 6.753 6.244 6.401 7.035 NOT TAKEN 6.479 6.637 6.276 6.791 5.955 6.118 6.354 NOT TAKEN 6.285 6.211 5.943 6.564 5.485 5.653 5.989 NOT TAKEN 5.924 5.948 5.727 5.685 5.406 5.402 5.016 NOT TAKEN 5.390 5.633 5.353 SPILLED 5.164 5.117 5.504 NOT TAKEN 5.232 5.383 5.578 5.488 4.826 4.867 5.172 NOT TAKEN 4.894 4.935 5.182 5.234 4.691 4.717 4.934 NOT TAKEN 4.732 4.716 5.319 5.044 GROUP: MALE 5 - 300 PPM C90971 C90972 C90973 C90974 C90975 C90976 C90977 C90978 C90979 C90980 C90981 C90982 C90983 C90984 C90985 C90986 C90987 C90988 C90989 C90990 24.897 20.706 24.808 25.267 26.653 27.721 25.432 24.512 23.634 27.166 26.854 24.967 25.557 24.262 25.895 24.342 24.055 33.527 25.567 24.296 19.850 18.227 21.264 20.896 23.673 21.962 20.537 21.359 20.679 23.140 22.885 22.093 26.346 20.370 22.768 21.695 20.428 24.531 21.526 21.635 18.417 17.651 18.980 18.554 21.984 19.378 19.657 SPILLED 18.023 21.085 20.190 19.887 21.556 19.102 19.017 19.075 17.787 21.156 19.114 20.566 18.157 16.301 18.088 17.883 19.486 18.348 17.348 17.554 16.619 19.545 18.255 17.681 19.286 NOT TAKEN 17.683 19.035 16.680 17.620 17.543 17.918 17.300 14.907 18.068 16.338 18.949 16.706 SPILLED 16.515 16.667 18.886 18.027 16.183 17.431 NOT TAKEN 17.376 SPILLED 16.122 SPILLED 16.327 17.722 18.141 14.198 17.016 15.729 18.666 15.731 SPILLED 15.233 15.332 17.486 16.853 16.610 17.512 NOT TAKEN 16.158 16.024 15.636 SPILLED 15.126 17.032 17.113 14.701 15.594 15.184 18.575 15.783 SPILLED 14.844 16.064 17.354 16.187 15.229 17.113 NOT TAKEN 16.493 15.672 16.051 SPILLED 15.236 16.271 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1390 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 300 PPM C90991 C90992 C90993 C90994 C90995 C90996 C90997 C90998 C90999 C91000 C91001 C91002 C91003 C91004 C91005 C91006 C91007 C91008 C91009 C91010 C91011 C91012 C91013 C91014 C91015 C91016 C91017 C91018 C91019 C91020 C91021 25.091 24.000 24.047 24.103 24.685 24.470 24.576 23.800 23.363 26.094 24.759 23.361 24.675 29.210 31.440 24.370 24.854 SPILLED 25.700 25.794 26.590 24.272 25.304 26.774 24.832 23.935 23.826 25.210 27.995 27.327 24.057 21.903 20.421 20.759 20.297 EXCLUDE 20.414 21.152 21.504 20.104 21.653 21.075 20.249 20.092 SPILLED 23.498 21.390 22.391 27.505 23.245 22.533 22.681 20.430 22.262 25.779 21.395 20.695 19.674 21.181 23.505 23.083 22.342 19.325 18.058 19.402 18.769 19.839 17.940 18.367 20.152 18.340 19.205 19.572 18.609 18.059 19.770 21.838 19.112 20.145 24.595 18.742 20.023 20.694 18.953 20.540 21.651 20.042 19.002 17.819 18.997 20.433 SPILLED 19.996 19.007 15.966 17.694 17.664 17.982 16.719 17.421 18.990 16.586 17.603 19.242 17.813 17.632 18.690 NOT TAKEN 17.590 19.171 SPILLED 16.437 NOT TAKEN 18.955 17.517 17.955 SPILLED 17.842 17.666 17.847 16.951 18.872 19.242 19.008 16.586 15.355 17.316 16.475 17.395 15.702 16.924 18.815 15.847 18.031 17.692 16.649 15.663 SPILLED NOT TAKEN 17.470 18.418 SPILLED 16.332 EXCLUDE 17.545 16.784 17.453 18.142 17.514 16.841 17.046 16.511 18.275 18.860 17.500 15.869 15.674 15.831 16.136 15.504 15.527 16.860 18.649 15.106 16.474 17.741 16.709 15.736 17.679 NOT TAKEN 16.873 18.237 SPILLED 16.841 16.792 16.210 15.119 16.583 17.421 16.741 15.584 16.079 15.120 17.691 18.331 16.625 16.146 15.276 15.120 15.566 16.065 15.453 16.404 18.098 15.275 15.983 16.246 16.064 NOT TAKEN 16.974 NOT TAKEN 15.150 17.187 SPILLED 16.185 16.722 15.982 15.652 17.065 17.167 16.906 16.146 16.152 14.570 16.162 17.366 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1391 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 300 PPM C91022 C91023 C91024 C91025 C91026 C91027 C91028 C91029 C91030 C91031 C91032 C91033 C91034 C91035 C91036 C91037 C91038 C91039 C91040 24.975 25.810 25.887 NOT TAKEN 24.031 24.490 24.905 24.653 26.674 22.800 27.968 24.734 27.086 26.294 23.688 24.695 29.116 26.418 24.899 21.691 22.175 22.316 21.486 20.201 21.664 20.620 21.502 23.060 20.615 23.377 21.289 22.295 21.801 18.583 21.202 20.652 22.697 21.705 19.021 20.039 19.305 19.177 SPILLED 19.143 18.429 18.699 20.294 18.678 20.546 19.163 20.647 19.245 19.123 18.727 18.852 19.923 20.530 18.782 19.355 NOT TAKEN 17.103 17.513 18.750 18.401 18.423 19.867 18.001 18.918 NOT TAKEN 19.790 18.559 17.298 NOT TAKEN 18.283 19.505 19.304 17.488 17.664 NOT TAKEN 16.336 17.120 17.040 17.448 17.017 17.989 16.105 18.890 NOT TAKEN 18.639 18.801 16.421 NOT TAKEN 16.827 18.763 18.739 16.811 17.618 NOT TAKEN 15.949 16.289 16.546 16.000 17.192 18.104 15.990 17.558 NOT TAKEN 17.691 17.650 15.617 NOT TAKEN 16.374 16.571 17.522 16.115 16.307 NOT TAKEN 14.998 16.092 15.112 15.548 NOT TAKEN 17.638 13.825 15.668 NOT TAKEN 16.364 16.504 15.105 NOT TAKEN 15.214 14.954 17.406 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN GROUP: MALE 6 - 100 PPM C91041 C91042 C91043 C91044 C91045 C91046 C91047 C91048 C91049 8.997 9.153 8.759 8.618 9.329 9.105 8.949 8.178 9.269 7.508 7.721 7.504 7.177 7.889 7.625 7.980 7.333 7.972 6.757 7.090 7.036 6.884 7.192 7.003 7.188 6.497 7.114 6.271 6.529 6.843 6.716 6.868 6.337 6.701 6.077 6.639 5.864 6.117 6.341 6.286 6.200 6.147 6.068 5.843 6.166 5.603 5.887 6.223 5.958 5.968 4.511 5.920 5.999 5.941 5.259 5.490 5.870 5.636 5.374 NOT TAKEN 5.573 5.570 5.685 5.144 5.315 5.883 5.519 5.007 NOT TAKEN 3.410 5.569 5.614 5.004 5.300 5.426 5.086 4.858 NOT TAKEN 4.936 5.493 5.411 4.775 5.393 5.401 5.329 4.690 NOT TAKEN 5.353 5.368 5.166 1392 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 100 PPM C91050 C91051 C91052 C91053 C91054 C91055 C91056 C91057 C91058 C91059 C91060 C91061 C91062 C91063 C91064 C91065 C91066 C91067 C91068 C91069 C91070 C91071 C91072 C91073 C91074 C91075 C91076 C91077 C91078 C91079 C91080 9.707 8.865 9.218 SPILLED 9.401 9.159 8.530 7.952 8.715 8.498 11.011 8.030 9.093 9.421 8.432 9.376 8.317 8.673 8.925 9.597 8.287 8.553 9.016 8.163 9.058 9.149 8.212 8.939 8.432 8.316 8.626 8.232 7.563 9.125 8.245 8.106 7.777 7.589 7.101 7.369 7.392 SPILLED 7.208 9.200 8.501 7.262 8.025 7.369 7.521 7.453 8.123 7.098 7.603 7.838 7.253 7.713 8.357 7.197 7.717 7.225 7.350 7.342 7.845 7.183 7.354 6.799 7.202 7.095 6.730 6.332 6.596 6.839 7.166 6.621 7.369 7.361 6.894 7.328 6.764 6.860 6.474 7.346 6.708 6.923 7.244 6.649 7.099 7.880 6.669 7.132 6.646 6.828 6.828 7.446 6.703 7.093 6.210 6.682 6.826 6.511 6.120 6.398 6.593 6.994 6.164 6.783 6.580 6.648 6.812 6.115 6.341 5.808 6.599 6.366 6.424 6.552 5.921 6.687 7.065 6.088 6.566 6.206 6.547 6.497 6.624 6.244 SPILLED 5.808 6.211 6.197 5.900 5.721 5.850 6.295 6.765 5.789 6.397 6.613 6.429 6.482 5.895 5.945 5.408 6.494 5.952 6.038 6.081 5.721 6.112 7.152 5.679 6.263 5.685 6.197 6.289 6.441 5.843 6.100 5.596 5.702 5.831 5.550 5.471 5.675 5.819 6.391 5.430 6.031 6.036 5.850 6.203 5.407 5.801 4.693 6.326 5.631 5.726 5.811 5.445 5.781 6.314 4.542 5.961 5.355 5.912 5.867 5.862 5.665 5.781 6.011 5.487 5.621 5.231 5.172 5.100 5.519 6.178 5.305 5.766 5.520 5.564 5.803 5.400 5.536 4.892 5.976 5.418 5.354 5.578 5.153 5.587 6.091 4.713 5.769 4.988 5.677 5.734 5.867 5.556 5.645 SPILLED 5.324 5.490 3.941 5.128 4.982 5.429 5.996 5.200 5.489 5.602 5.328 5.517 5.448 5.399 4.607 5.943 5.242 5.283 5.398 5.139 5.260 5.817 5.660 5.410 4.827 5.365 5.592 4.909 5.590 5.854 4.961 4.856 5.023 5.666 4.786 4.651 5.075 5.529 5.005 5.152 4.971 4.913 5.183 5.040 5.085 1.897 5.465 5.007 5.054 5.054 4.699 5.026 5.351 5.317 5.252 4.511 4.941 5.353 5.064 5.489 5.190 4.808 4.510 4.937 5.510 4.613 4.308 5.020 5.507 4.873 4.820 4.786 4.841 4.977 4.936 4.872 2.659 5.216 4.868 4.877 4.900 4.819 4.832 5.340 5.135 5.031 4.478 4.833 5.140 1393 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 7 - 300 PPM C91081 C91082 C91083 C91084 C91085 C91086 C91087 C91088 C91089 C91090 C91091 C91092 C91093 C91094 C91095 C91096 C91097 C91098 C91099 C91100 C91101 C91102 C91103 C91104 C91105 C91106 C91107 C91108 C91109 C91110 C91111 SPILLED 24.176 25.378 23.153 SPILLED 23.981 25.314 24.697 27.211 22.989 24.880 25.369 23.870 25.200 24.320 22.812 25.730 23.754 25.866 25.525 23.983 25.040 26.267 26.748 24.207 23.629 25.787 27.042 26.087 26.204 24.901 22.053 20.510 21.467 20.045 23.239 20.583 21.680 21.978 22.983 21.601 21.429 20.893 20.168 21.129 20.041 20.658 20.816 20.739 20.438 22.153 20.530 27.843 21.974 24.620 22.452 20.515 21.995 23.579 23.252 22.254 21.466 19.728 18.418 19.613 17.937 20.160 19.991 18.358 20.311 20.408 19.452 19.226 18.719 18.803 18.597 SPILLED 18.459 18.694 19.323 19.495 19.643 18.850 20.849 20.401 21.304 18.158 18.386 19.521 21.258 20.085 21.131 19.391 23.859 17.062 18.407 16.706 19.770 18.553 16.866 18.595 19.188 17.710 19.103 17.900 14.074 16.770 18.618 17.170 17.191 18.057 21.504 18.656 18.527 18.617 19.226 19.048 16.912 15.451 18.455 19.585 18.616 18.650 17.996 17.552 16.544 17.775 16.698 16.881 17.498 16.117 16.848 18.398 17.481 17.808 16.358 18.185 16.598 18.175 17.168 16.568 17.188 16.789 17.267 16.930 19.103 18.423 18.833 16.650 17.689 17.506 17.912 17.831 18.175 17.695 16.568 15.365 18.139 16.349 15.467 16.278 14.870 15.882 18.034 16.446 16.426 16.552 17.824 16.889 17.734 16.173 15.625 16.140 NOT TAKEN 16.186 17.040 18.780 17.799 17.863 16.707 16.229 16.967 17.535 18.279 17.574 17.812 15.746 15.449 17.093 16.188 13.984 15.424 14.625 15.563 16.327 16.786 15.667 14.934 16.685 15.841 15.807 15.407 15.158 15.964 NOT TAKEN 16.298 17.523 17.143 16.432 16.892 16.479 16.725 16.309 16.765 16.184 15.988 17.304 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1394 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 7 - 300 PPM C91112 C91113 C91114 C91115 C91116 C91117 C91118 C91119 C91120 26.190 26.503 25.605 25.227 23.390 27.090 26.607 25.788 26.823 22.487 21.467 21.087 21.971 19.972 SPILLED 21.872 22.338 22.313 20.140 19.847 19.237 20.285 18.201 SPILLED 20.018 20.106 20.741 18.599 17.586 16.631 18.399 16.450 21.853 18.269 18.297 18.855 17.955 16.603 16.633 16.637 16.881 SPILLED 17.640 17.921 18.274 17.081 15.572 16.034 16.775 15.899 18.449 18.319 17.182 14.926 16.464 17.062 15.029 15.873 18.320 SPILLED 15.177 16.327 3.588 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN GROUP: FEMALE 2 - 3 PPM C91191 C91192 C91193 C91194 C91195 C91196 C91197 C91198 C91199 C91200 C91201 C91202 C91203 C91204 C91205 C91206 C91207 C91208 C91209 0.264 0.319 SPILLED 0.277 0.306 0.280 0.312 0.276 0.381 0.283 0.269 0.275 0.308 0.304 0.325 SPILLED 0.306 0.294 0.267 0.249 0.285 SPILLED 0.257 0.291 0.279 0.297 0.243 0.353 0.269 SPILLED 0.265 0.297 0.281 0.307 0.262 0.274 0.268 0.244 0.227 0.254 0.565 0.250 0.263 0.265 0.270 0.247 0.298 0.291 0.246 0.238 0.281 0.266 0.298 0.244 0.238 0.241 0.239 0.223 0.284 SPILLED 0.235 0.248 0.228 0.291 NOT TAKEN SPILLED 0.337 0.271 0.240 0.270 0.258 NOT TAKEN 0.229 0.263 SPILLED 0.216 0.232 0.232 SPILLED 0.237 0.227 0.214 0.293 NOT TAKEN 0.310 0.256 0.227 0.208 0.246 0.252 NOT TAKEN 0.226 0.245 0.232 0.206 0.205 0.255 SPILLED 0.245 0.234 0.204 SPILLED NOT TAKEN SPILLED 0.233 0.279 0.193 0.219 0.261 NOT TAKEN 0.216 0.235 0.233 0.208 0.232 0.225 SPILLED 0.231 0.235 0.209 SPILLED NOT TAKEN SPILLED 0.194 0.215 0.185 0.202 0.235 NOT TAKEN 0.213 0.233 0.213 0.196 0.212 0.237 SPILLED 0.213 0.208 0.185 0.221 NOT TAKEN SPILLED 0.225 SPILLED 0.175 0.197 0.282 NOT TAKEN 0.204 0.225 SPILLED 0.198 0.204 0.213 SPILLED 0.214 0.188 0.169 0.229 NOT TAKEN SPILLED 0.228 0.180 0.168 0.174 0.210 NOT TAKEN 0.188 0.213 0.194 0.183 0.190 0.202 0.258 0.210 0.192 0.167 0.271 NOT TAKEN SPILLED 0.241 0.199 0.167 0.168 0.234 NOT TAKEN 0.194 0.198 0.214 0.188 1395 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91210 C91211 C91212 C91213 C91214 C91215 C91216 C91217 C91218 C91219 C91220 C91221 C91222 C91223 C91224 C91225 C91226 C91227 C91228 C91229 C91230 C91231 C91232 C91233 C91234 C91235 C91236 C91237 C91238 C91239 C91240 0.453 0.289 0.270 0.287 0.329 0.292 0.301 0.274 0.277 0.327 0.299 0.320 0.299 0.275 0.408 0.326 0.259 SPILLED 0.296 0.295 0.325 0.303 0.291 0.282 0.278 0.346 0.316 0.374 0.294 0.283 0.312 0.337 0.272 0.250 0.286 0.301 0.268 SPILLED 0.267 0.341 0.296 0.289 0.291 0.278 0.254 0.388 0.277 0.251 0.241 0.263 0.294 0.297 0.286 0.281 0.258 0.279 SPILLED 0.289 0.336 0.271 0.254 0.248 0.323 0.241 0.242 0.263 0.272 0.254 SPILLED 0.255 SPILLED 0.270 0.264 0.261 0.251 0.262 0.278 0.285 0.247 SPILLED 0.234 0.260 0.275 0.260 0.248 0.242 0.252 0.410 0.286 0.273 0.244 0.267 0.256 SPILLED 0.243 0.235 0.248 0.245 0.233 SPILLED 0.256 SPILLED SPILLED 0.234 0.245 NOT TAKEN 0.259 SPILLED 0.202 0.216 SPILLED 0.283 0.237 NOT TAKEN SPILLED 0.262 0.265 0.235 SPILLED 0.254 NOT TAKEN 0.237 0.242 0.231 SPILLED SPILLED SPILLED 0.224 0.243 0.215 SPILLED 0.235 0.262 0.301 0.221 0.233 NOT TAKEN 0.252 0.274 0.220 0.229 SPILLED 0.249 0.223 NOT TAKEN 0.220 0.212 0.240 0.226 SPILLED 0.248 NOT TAKEN 0.221 0.230 0.224 SPILLED 0.306 0.212 0.219 0.234 0.224 0.270 0.230 SPILLED SPILLED 0.217 0.266 NOT TAKEN 0.232 0.249 0.217 0.225 0.205 0.226 0.208 NOT TAKEN 0.236 0.213 0.238 0.218 SPILLED 0.302 NOT TAKEN 0.225 0.228 0.217 0.245 0.209 0.205 0.221 0.219 0.206 SPILLED 0.241 0.256 SPILLED 0.188 SPILLED NOT TAKEN 0.223 0.258 0.216 0.220 0.222 0.216 0.192 NOT TAKEN 0.195 0.215 0.223 0.193 SPILLED SPILLED NOT TAKEN 0.224 0.221 0.222 SPILLED 0.196 0.195 0.209 0.217 0.213 SPILLED 0.218 0.317 0.329 0.194 0.228 NOT TAKEN 0.229 SPILLED 0.162 0.217 0.195 0.222 0.200 NOT TAKEN 0.194 0.211 0.232 0.191 SPILLED 0.229 NOT TAKEN 0.195 0.206 0.206 SPILLED 0.190 0.175 0.197 0.197 0.200 SPILLED 0.202 0.285 SPILLED 0.175 0.213 NOT TAKEN 0.203 0.221 0.174 0.205 0.176 0.200 0.178 NOT TAKEN 0.181 0.198 0.196 0.193 0.313 0.193 NOT TAKEN 0.190 0.185 0.198 SPILLED 0.184 0.181 0.194 0.188 0.188 SPILLED 0.200 SPILLED SPILLED 0.174 0.195 NOT TAKEN 0.201 0.221 0.196 0.193 0.175 0.192 0.170 NOT TAKEN 0.179 0.186 0.184 0.175 SPILLED 0.199 NOT TAKEN 0.185 0.187 0.183 1396 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91241 C91242 C91243 C91244 C91245 C91246 C91247 C91248 C91249 C91250 0.300 SPILLED 0.320 0.287 0.344 0.394 0.307 0.289 0.313 0.308 0.274 0.371 0.330 0.271 0.342 0.332 0.296 0.266 SPILLED 0.274 0.247 SPILLED 0.311 0.258 0.341 SPILLED SPILLED 0.238 0.259 SPILLED 0.252 0.384 SPILLED 0.239 0.369 SPILLED 0.260 0.233 0.336 0.237 0.233 SPILLED 0.271 0.223 0.256 SPILLED 0.263 0.218 0.252 0.238 SPILLED SPILLED SPILLED 0.228 0.295 SPILLED 0.271 0.209 SPILLED SPILLED 0.216 SPILLED 0.227 0.231 SPILLED SPILLED 0.270 0.217 0.230 0.213 0.312 0.300 SPILLED 0.213 0.258 SPILLED 0.292 0.202 0.229 0.321 0.188 SPILLED 0.240 0.177 0.271 SPILLED SPILLED 0.176 0.199 0.188 SPILLED SPILLED 0.271 0.189 SPILLED SPILLED SPILLED 0.185 0.201 0.276 GROUP: FEMALE 3 - 30 PPM C91251 C91252 C91253 C91254 C91255 C91256 C91257 C91258 C91259 C91260 C91261 C91262 C91263 C91264 C91265 C91266 C91267 C91268 2.525 2.988 2.816 3.093 2.807 3.358 2.507 2.980 3.960 2.822 2.796 SPILLED 3.053 3.009 2.903 3.167 3.068 3.057 2.301 2.709 2.783 2.938 2.777 2.608 2.487 2.907 SPILLED 2.651 2.542 2.452 2.703 2.772 2.605 2.885 2.776 SPILLED 2.263 2.503 2.319 2.679 2.632 SPILLED 2.700 2.583 2.561 2.371 SPILLED 2.260 2.642 SPILLED 2.318 2.650 2.571 2.403 NOT TAKEN 2.391 2.536 2.555 3.030 2.573 SPILLED 2.234 2.522 2.273 2.688 2.400 2.446 2.500 2.778 2.692 2.512 2.458 NOT TAKEN 2.387 2.336 2.571 2.605 2.521 2.035 2.391 2.277 2.275 2.359 2.060 2.258 2.329 3.310 2.546 2.312 2.309 NOT TAKEN 2.236 2.315 2.468 2.830 2.242 2.067 2.261 2.160 2.194 2.355 1.987 2.177 2.201 2.130 2.571 2.262 2.129 NOT TAKEN 2.115 2.002 2.313 2.463 2.118 1.833 2.008 2.105 2.028 2.298 1.909 1.969 2.027 2.176 2.143 2.067 1.919 NOT TAKEN 2.323 2.275 2.325 2.535 2.172 1.987 2.055 2.224 1.717 2.488 2.309 2.078 2.043 2.050 2.071 2.128 1.871 NOT TAKEN 2.129 1.964 2.003 2.374 2.068 1.681 1.980 2.052 1.895 2.199 1.825 2.065 1.964 1.850 2.049 1.939 1.866 NOT TAKEN 1.944 2.019 2.121 2.257 1.818 SPILLED 1.886 2.008 1.987 1.751 1.695 1.889 1.815 SPILLED 1.888 1.815 1.811 1397 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 30 PPM C91269 C91270 C91271 C91272 C91273 C91274 C91275 C91276 C91277 C91278 C91279 C91280 C91281 C91282 C91283 C91284 C91285 C91286 C91287 C91288 C91289 C91290 C91291 C91292 C91293 C91294 C91295 C91296 C91297 C91298 C91299 2.973 3.514 3.052 3.150 SPILLED 2.781 2.976 2.785 2.930 2.896 3.411 3.176 2.799 3.190 3.188 3.227 2.542 2.637 4.219 2.574 SPILLED 2.774 3.136 2.841 3.522 3.183 3.056 2.608 4.201 2.924 SPILLED 2.796 3.012 SPILLED 2.911 3.565 2.674 2.921 2.626 2.643 SPILLED 3.617 2.835 2.821 2.857 2.872 2.965 2.297 2.474 2.634 2.350 2.945 2.434 2.614 2.647 4.044 2.864 3.433 SPILLED SPILLED 2.523 SPILLED 2.399 2.888 2.555 2.383 SPILLED 2.359 2.517 2.558 2.434 SPILLED SPILLED 2.628 SPILLED 2.807 2.708 2.825 2.071 2.452 3.033 2.179 SPILLED 2.346 2.432 2.556 3.111 2.571 3.688 SPILLED SPILLED 2.297 2.588 2.459 2.669 SPILLED NOT TAKEN 3.659 SPILLED 2.482 2.398 2.128 2.550 2.586 2.460 SPILLED 2.662 2.465 2.729 NOT TAKEN 2.650 NOT TAKEN 2.036 2.449 2.322 NOT TAKEN 2.618 SPILLED 2.545 SPILLED 2.580 2.441 2.429 2.547 2.440 2.551 3.472 NOT TAKEN 2.889 SPILLED 2.620 2.208 2.366 2.488 2.335 2.343 SPILLED 2.700 2.426 2.538 NOT TAKEN 2.508 NOT TAKEN 2.052 2.382 2.042 NOT TAKEN 2.214 SPILLED 2.341 2.870 2.262 2.621 2.369 2.365 2.119 2.468 2.831 NOT TAKEN SPILLED SPILLED 2.219 2.211 2.286 SPILLED 2.445 2.143 SPILLED 2.571 2.461 2.383 NOT TAKEN SPILLED NOT TAKEN 1.830 2.220 2.022 NOT TAKEN 2.039 SPILLED 2.100 SPILLED 2.262 2.680 2.123 2.403 2.186 2.288 SPILLED NOT TAKEN SPILLED 2.120 2.193 2.269 2.090 2.151 2.282 2.155 SPILLED 2.358 2.135 2.118 NOT TAKEN 2.921 NOT TAKEN 1.746 2.076 2.184 NOT TAKEN 2.010 SPILLED 2.080 2.400 2.167 2.719 1.979 2.163 SPILLED 2.268 SPILLED NOT TAKEN 2.525 SPILLED SPILLED 2.111 1.993 SPILLED 2.010 2.070 SPILLED 2.182 1.940 SPILLED NOT TAKEN 2.284 NOT TAKEN 1.762 2.151 2.018 NOT TAKEN 2.088 SPILLED SPILLED 3.819 2.355 2.188 2.036 2.128 2.092 2.078 2.143 NOT TAKEN SPILLED SPILLED SPILLED 2.016 1.788 1.795 1.939 2.111 SPILLED 2.184 2.057 2.112 NOT TAKEN 2.250 NOT TAKEN 1.708 1.843 1.915 NOT TAKEN 1.815 1.901 1.908 2.386 SPILLED SPILLED 1.967 1.920 1.959 1.997 SPILLED NOT TAKEN 2.345 2.526 2.211 1.967 1.837 2.085 2.037 1.942 SPILLED 2.025 1.993 1.952 NOT TAKEN 2.202 NOT TAKEN 1.599 1.937 1.733 NOT TAKEN 1.791 SPILLED 1.960 2.155 2.053 3.000 1.864 2.054 1398 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 30 PPM C91300 C91301 C91302 C91303 C91304 C91305 C91306 C91307 C91308 C91309 C91310 4.247 3.110 SPILLED 2.966 3.264 3.069 SPILLED 2.842 3.051 3.010 3.027 SPILLED 2.720 SPILLED 2.788 2.877 2.777 SPILLED 2.757 2.850 2.772 2.760 3.823 2.710 SPILLED 2.571 2.593 2.525 2.857 2.454 2.741 2.594 2.420 SPILLED 2.449 SPILLED 2.469 2.504 2.742 SPILLED 2.584 2.673 2.412 2.542 SPILLED 2.352 SPILLED 2.204 2.503 2.660 2.293 2.237 2.522 2.437 2.609 3.436 2.299 SPILLED 2.104 SPILLED 2.349 SPILLED 2.271 2.665 2.282 2.235 2.818 2.147 2.207 2.008 2.309 2.325 SPILLED 1.968 2.553 2.275 2.249 3.680 2.220 SPILLED 1.826 2.380 2.360 SPILLED 2.490 2.621 2.058 2.202 SPILLED 2.053 1.795 1.695 2.005 2.101 1.972 1.802 2.832 1.965 2.280 SPILLED 2.095 SPILLED 1.871 2.038 2.136 1.915 1.735 SPILLED 1.949 2.017 GROUP: FEMALE 4 - 100 PPM C91311 C91312 C91313 C91314 C91315 C91316 C91317 C91318 C91319 C91320 C91321 C91322 C91323 C91324 C91325 C91326 C91327 9.075 8.684 10.671 9.784 10.365 SPILLED 9.632 11.044 11.913 9.928 10.174 9.824 9.605 10.074 8.376 10.141 9.726 10.540 8.337 11.006 9.785 SPILLED SPILLED 8.693 10.967 10.661 SPILLED 10.106 9.574 8.386 8.909 7.431 8.489 8.627 SPILLED 7.351 SPILLED 9.134 9.315 9.335 7.961 9.746 8.914 SPILLED 9.005 SPILLED SPILLED 8.143 7.295 9.467 8.070 9.705 7.614 8.513 8.850 8.355 NOT TAKEN NOT TAKEN SPILLED 9.501 9.259 11.894 9.012 7.822 8.317 7.143 9.027 7.774 SPILLED 7.038 SPILLED SPILLED 8.122 NOT TAKEN NOT TAKEN 6.897 8.430 7.992 8.442 8.307 SPILLED 7.379 6.342 8.153 8.682 10.039 6.604 SPILLED SPILLED 8.304 NOT TAKEN NOT TAKEN 6.469 8.207 7.021 SPILLED 8.443 8.436 7.235 6.336 7.766 7.080 7.243 6.563 7.099 SPILLED 8.163 NOT TAKEN NOT TAKEN 6.329 7.337 6.823 7.478 7.317 SPILLED 7.281 6.143 8.173 7.049 7.685 6.769 8.503 SPILLED 7.995 NOT TAKEN NOT TAKEN 6.369 7.158 6.701 SPILLED 7.649 7.259 7.278 6.167 7.412 7.143 SPILLED 6.608 7.296 SPILLED 7.729 NOT TAKEN NOT TAKEN 5.957 6.899 6.389 7.061 7.336 6.665 6.620 5.992 6.730 6.553 6.682 6.198 SPILLED SPILLED 7.603 NOT TAKEN NOT TAKEN 5.426 6.789 6.467 6.676 6.681 6.501 6.151 5.419 6.671 6.531 1399 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91328 C91329 C91330 C91331 C91332 C91333 C91334 C91335 C91336 C91337 C91338 C91339 C91340 C91341 C91342 C91343 C91344 C91345 C91346 C91347 C91348 C91349 C91350 C91351 C91352 C91353 C91354 C91355 C91356 C91357 C91358 10.561 10.488 9.524 10.832 SPILLED 9.324 10.813 9.846 SPILLED 9.030 14.148 9.945 10.695 9.680 10.016 10.405 8.571 SPILLED SPILLED SPILLED 10.428 SPILLED SPILLED 9.003 9.744 9.909 11.379 11.264 10.724 8.946 6.524 9.228 9.470 8.517 9.140 9.654 9.159 9.422 9.073 SPILLED 7.677 SPILLED 8.656 9.882 8.378 8.204 9.423 7.705 8.665 10.330 SPILLED 8.990 8.929 SPILLED 8.781 8.295 9.197 SPILLED 11.251 9.689 7.979 8.980 7.849 8.740 8.265 9.363 9.594 SPILLED 8.565 8.601 SPILLED 7.081 10.851 8.571 8.947 8.388 7.291 7.862 7.255 7.877 8.506 SPILLED 8.383 8.096 SPILLED 7.499 8.043 7.836 SPILLED 9.104 8.969 7.857 8.022 8.163 9.069 7.877 8.571 8.658 NOT TAKEN 7.734 7.587 SPILLED 7.634 9.790 8.350 SPILLED 7.903 7.222 7.963 7.189 NOT TAKEN 8.414 SPILLED 7.976 7.816 NOT TAKEN 7.436 7.578 7.966 SPILLED 9.432 9.856 7.199 7.792 7.300 7.567 6.996 8.349 8.411 NOT TAKEN 7.067 7.192 SPILLED 6.853 7.212 7.206 8.163 7.157 7.586 7.734 6.674 NOT TAKEN 7.657 7.038 7.143 7.975 NOT TAKEN 6.975 7.075 7.615 SPILLED 8.237 SPILLED 6.752 7.308 6.701 7.284 7.334 7.744 7.424 NOT TAKEN 7.012 7.542 SPILLED 6.757 7.412 7.338 7.407 6.906 6.775 7.787 6.252 NOT TAKEN 7.226 6.892 7.127 7.023 NOT TAKEN 7.566 7.338 6.832 SPILLED 7.561 7.418 6.458 6.719 6.507 6.975 6.617 7.255 6.973 NOT TAKEN 6.529 6.973 SPILLED 6.262 7.358 6.494 6.676 6.415 7.128 6.980 6.611 NOT TAKEN 7.210 6.647 6.820 6.643 NOT TAKEN 6.699 6.690 7.437 6.149 7.430 7.279 6.449 6.748 6.554 6.905 6.598 7.280 8.905 NOT TAKEN 6.349 6.932 SPILLED 6.634 6.746 7.114 6.478 6.367 7.057 6.706 6.103 NOT TAKEN 6.859 6.756 6.105 7.085 NOT TAKEN 6.559 6.168 6.809 6.389 7.654 SPILLED 6.267 6.798 6.275 6.349 6.434 6.710 7.735 NOT TAKEN 5.802 6.862 16.507 5.887 5.781 6.130 6.061 5.982 7.058 6.767 6.166 NOT TAKEN 6.429 6.202 6.070 7.537 NOT TAKEN 6.526 5.950 6.709 5.750 7.664 6.418 5.871 6.984 6.001 6.184 6.196 6.958 6.466 NOT TAKEN 5.975 6.531 10.286 6.094 6.609 6.059 5.810 5.940 6.392 6.478 6.064 NOT TAKEN 6.489 6.038 6.477 6.185 NOT TAKEN 6.095 5.802 6.621 5.893 7.630 6.130 5.559 6.637 1400 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91359 C91360 C91361 C91362 C91363 C91364 C91365 C91366 C91367 C91368 C91369 C91370 C91371 C91372 C91373 C91374 C91375 C91376 C91377 C91378 C91379 C91380 11.650 10.494 SPILLED 10.767 10.824 10.714 SPILLED 9.952 10.884 11.052 9.552 11.285 8.804 12.281 9.996 SPILLED 10.799 9.923 10.141 9.470 10.381 10.019 SPILLED 10.382 8.780 12.662 9.852 10.504 10.870 8.079 9.353 11.843 8.702 10.766 8.399 9.125 8.962 SPILLED 9.098 8.618 9.159 8.586 13.551 8.837 9.219 SPILLED 7.602 11.638 8.940 8.856 SPILLED 8.399 8.372 SPILLED 8.092 9.115 7.623 10.218 7.799 8.172 8.302 7.838 8.621 7.761 8.864 8.450 8.466 8.112 7.367 SPILLED 8.618 8.429 SPILLED 8.010 7.198 10.575 7.774 8.718 7.246 8.484 8.084 SPILLED 8.630 7.293 8.725 7.645 8.865 7.714 7.798 7.074 6.784 SPILLED 7.129 7.427 SPILLED 7.143 7.237 9.039 8.098 8.479 7.254 8.595 7.390 7.326 7.685 7.351 7.954 7.640 8.682 7.403 7.891 7.360 6.641 SPILLED 7.671 6.965 8.163 7.615 7.417 7.281 7.611 7.861 6.764 6.883 7.079 6.908 7.188 6.911 7.489 7.234 7.957 6.954 8.584 6.865 6.029 8.453 7.143 6.095 7.728 6.862 6.292 7.024 7.067 7.259 SPILLED 6.773 6.875 5.921 6.593 6.455 7.627 7.009 7.051 6.800 SPILLED 6.842 5.209 SPILLED 7.247 6.294 9.201 6.874 7.130 6.647 6.848 7.536 SPILLED SPILLED 6.712 6.142 6.808 6.580 7.490 6.478 6.645 6.774 SPILLED 6.531 6.009 6.209 6.470 5.811 SPILLED 6.209 5.804 5.819 6.759 7.351 SPILLED 6.087 6.520 6.126 6.803 5.991 6.925 5.933 7.053 6.500 SPILLED 6.877 6.026 SPILLED 6.218 5.822 SPILLED 5.450 5.979 5.864 6.627 6.803 8.821 6.379 6.009 5.527 6.202 5.969 6.545 6.272 7.026 6.981 GROUP: FEMALE 5 - 300 PPM C91381 C91382 C91383 C91384 C91385 C91386 SPILLED 25.325 27.763 42.857 SPILLED 29.196 23.851 25.850 24.926 25.835 26.925 SPILLED 22.067 19.540 23.506 21.601 22.061 SPILLED 23.822 21.617 23.129 25.919 23.920 SPILLED 21.697 19.951 21.537 SPILLED 23.044 SPILLED 20.911 21.182 21.376 22.489 30.537 SPILLED 19.563 18.940 18.578 SPILLED 24.965 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1401 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 300 PPM C91387 C91388 C91389 C91390 C91391 C91392 C91393 C91394 C91395 C91396 C91397 C91398 C91399 C91400 C91401 C91402 C91403 C91404 C91405 C91406 C91407 C91408 C91409 C91410 C91411 C91412 C91413 C91414 C91415 C91416 C91417 25.075 27.940 29.132 29.972 27.479 SPILLED 24.409 25.761 36.029 SPILLED 33.653 29.432 27.857 28.496 30.286 26.025 SPILLED 25.907 28.042 27.318 29.732 27.786 SPILLED SPILLED 30.521 28.992 SPILLED 30.062 25.476 24.333 30.938 20.420 25.038 19.821 25.661 23.953 26.429 41.409 24.164 31.906 SPILLED 25.356 22.632 22.842 24.154 32.666 22.785 34.689 22.651 24.561 24.176 23.795 25.108 20.513 29.748 26.356 23.160 SPILLED 26.698 21.548 23.489 SPILLED 18.420 22.500 19.540 25.739 21.652 SPILLED SPILLED 21.153 42.730 19.826 24.196 23.294 20.029 25.127 23.240 21.951 30.064 21.730 22.257 21.138 23.903 22.192 19.524 21.310 22.534 22.568 SPILLED 22.799 22.309 19.525 24.330 18.182 22.199 20.733 24.922 21.429 20.526 19.927 20.346 24.735 21.574 24.039 21.534 18.932 25.676 22.231 22.120 SPILLED 21.831 22.047 SPILLED 21.659 SPILLED 17.946 19.303 21.277 19.348 SPILLED 21.481 18.033 18.543 23.713 23.209 20.285 18.299 22.991 19.275 22.238 18.016 19.167 SPILLED 17.945 23.461 19.952 18.367 24.570 20.698 20.269 SPILLED 20.377 22.127 20.324 21.373 21.709 19.833 19.073 20.145 20.879 21.889 22.963 19.955 19.068 20.295 17.914 19.266 19.022 24.238 18.642 19.733 17.623 18.624 22.164 18.273 22.709 20.168 18.845 24.437 20.465 20.639 SPILLED 20.786 22.053 19.665 SPILLED 19.414 17.582 18.614 20.045 20.339 SPILLED 21.334 18.634 19.027 19.403 17.680 19.929 19.728 23.085 18.781 20.227 18.477 18.002 22.164 17.433 20.580 18.849 19.113 23.041 20.000 20.533 SPILLED 19.395 21.176 19.395 20.596 19.126 18.431 18.729 18.182 17.840 18.522 19.027 18.950 17.424 19.395 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1402 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 20 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 300 PPM C91418 C91419 C91420 C91421 C91422 C91423 C91424 C91425 C91426 C91427 C91428 C91429 C91430 C91431 C91432 C91433 C91434 C91435 C91436 C91437 C91438 C91439 C91440 C91441 C91442 C91443 C91444 C91445 C91446 C91447 C91448 25.835 SPILLED 29.630 26.804 SPILLED 25.051 25.815 SPILLED 30.777 29.435 30.192 27.862 29.227 28.220 21.599 39.110 28.398 28.083 23.003 26.802 31.504 33.236 SPILLED 30.000 28.656 25.691 30.458 SPILLED 32.180 30.959 29.011 24.290 SPILLED 32.688 24.568 20.665 25.237 24.702 22.135 38.884 SPILLED 25.786 22.870 26.802 22.414 22.843 34.421 25.051 25.876 24.339 24.390 27.607 26.037 25.893 25.032 24.213 21.542 24.852 25.210 27.706 24.697 SPILLED 23.785 22.206 23.233 21.237 19.694 23.915 21.486 19.676 23.174 23.100 25.355 19.515 24.702 20.714 19.328 SPILLED 22.432 24.242 21.800 21.861 24.773 22.637 22.388 21.188 23.209 21.641 24.010 SPILLED 24.529 22.350 SPILLED 21.866 SPILLED 22.294 19.514 17.143 NOT TAKEN 19.831 17.811 17.197 NOT TAKEN 22.857 19.565 NOT TAKEN SPILLED 21.275 SPILLED 21.884 23.433 20.351 21.581 24.122 SPILLED 21.792 21.943 22.148 NOT TAKEN 23.068 20.251 23.957 20.589 NOT TAKEN 21.853 19.780 19.748 20.375 15.761 NOT TAKEN 19.500 18.701 21.429 NOT TAKEN 21.932 19.988 NOT TAKEN SPILLED 20.887 SPILLED 20.977 21.922 18.267 20.455 23.336 20.168 19.811 20.991 20.779 NOT TAKEN 21.700 20.100 24.454 21.320 NOT TAKEN 19.748 18.792 19.527 18.653 16.459 NOT TAKEN 19.462 17.401 25.663 NOT TAKEN 20.203 18.279 NOT TAKEN SPILLED 19.222 18.169 19.398 21.188 21.257 19.868 22.091 20.121 19.580 20.387 21.481 NOT TAKEN 20.896 SPILLED 22.619 19.683 NOT TAKEN 19.162 18.883 18.960 18.737 16.396 NOT TAKEN 18.440 17.430 19.951 NOT TAKEN 20.750 17.073 NOT TAKEN SPILLED 20.111 19.764 19.121 21.287 19.797 20.157 21.590 17.832 20.417 19.845 19.208 NOT TAKEN 19.748 19.216 22.796 25.714 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1403 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 21 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 300 PPM C91449 C91450 28.410 24.838 29.635 22.619 SPILLED 21.772 SPILLED 19.023 36.882 15.345 SPILLED 17.009 SPILLED 15.824 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN GROUP: FEMALE 6 - 100 PPM C91451 C91452 C91453 C91454 C91455 C91456 C91457 C91458 C91459 C91460 C91461 C91462 C91463 C91464 C91465 C91466 C91467 C91468 C91469 C91470 C91471 C91472 C91473 C91474 C91475 C91476 9.055 9.416 11.003 10.282 10.703 10.671 10.909 9.321 9.716 9.460 8.668 10.834 SPILLED 9.958 10.919 9.301 10.367 8.530 9.049 10.417 10.244 9.905 9.439 11.077 13.870 SPILLED 8.427 8.498 9.577 8.891 12.166 8.516 9.624 8.041 9.870 9.041 8.369 9.472 8.269 8.965 SPILLED 7.852 11.018 7.750 8.055 9.967 8.696 8.242 8.019 10.352 11.136 8.665 7.526 7.798 8.429 8.011 10.507 7.678 7.872 8.442 7.579 7.988 7.870 8.954 7.048 8.457 9.704 8.019 7.600 7.833 7.906 8.727 8.271 8.880 7.740 8.889 10.151 7.854 7.875 7.758 8.605 7.861 8.212 7.741 8.008 7.212 7.965 7.546 7.565 8.317 6.806 8.184 8.498 7.787 8.403 7.362 7.077 8.016 7.889 9.377 7.420 8.910 SPILLED 7.961 7.937 7.366 8.108 7.373 7.834 7.212 7.770 7.471 7.727 7.362 7.292 8.059 6.775 7.929 8.578 6.745 7.434 7.231 6.934 8.179 7.914 9.464 7.410 6.930 8.239 7.509 7.191 6.624 7.558 6.643 7.448 6.777 7.519 6.745 SPILLED 6.926 6.956 7.701 5.864 7.094 8.598 5.832 8.367 7.042 6.570 7.849 7.481 7.418 6.775 7.852 8.760 7.026 6.782 7.044 7.158 6.752 7.302 6.356 6.821 6.520 6.004 6.117 6.162 7.615 6.223 6.667 7.094 6.472 7.128 6.152 6.276 6.491 7.000 7.072 7.393 7.236 10.430 6.776 8.608 7.170 7.483 6.369 8.092 6.482 7.323 6.909 SPILLED 6.477 6.732 7.545 5.725 6.842 8.312 6.247 SPILLED 7.718 6.401 7.842 6.835 SPILLED SPILLED 8.437 SPILLED 6.657 6.222 6.268 6.667 5.799 7.008 6.162 6.790 6.071 6.589 6.148 6.038 6.902 5.571 6.398 6.623 6.200 6.272 5.941 6.027 6.836 6.754 SPILLED 6.555 7.268 6.920 6.361 6.589 6.714 6.726 6.389 6.850 6.471 6.498 6.030 SPILLED 7.397 5.961 6.732 5.378 6.559 6.708 5.558 6.483 7.508 6.012 7.046 6.674 7.290 7.809 SPILLED SPILLED 6.469 1404 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 22 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 100 PPM C91477 C91478 C91479 C91480 C91481 C91482 C91483 C91484 C91485 C91486 C91487 C91488 C91489 C91490 8.891 SPILLED 12.841 10.149 10.492 10.268 11.632 8.571 9.668 9.288 11.276 NOT TAKEN 8.496 10.174 8.041 10.396 11.287 8.833 9.467 9.470 9.085 7.933 9.102 8.102 8.809 9.058 8.108 9.473 7.746 9.278 9.242 8.304 8.933 9.233 10.190 7.451 8.318 7.841 7.870 8.120 8.204 8.579 SPILLED 8.103 9.003 7.615 8.163 8.823 SPILLED 7.127 7.651 7.712 7.326 SPILLED 8.231 SPILLED 7.339 7.565 8.417 7.094 7.583 8.026 8.778 7.128 7.323 6.963 7.079 8.583 7.175 7.926 6.794 6.918 7.611 6.782 7.159 7.578 6.514 6.678 7.254 6.427 6.418 6.729 6.847 7.174 7.220 6.797 7.337 6.551 6.574 7.219 7.426 6.064 6.446 6.394 6.282 6.396 6.694 7.127 6.814 SPILLED 8.015 6.257 6.425 7.011 SPILLED 6.642 7.008 6.050 6.630 6.390 7.005 7.098 6.480 7.345 7.370 6.160 6.424 6.773 6.325 6.126 6.148 5.998 5.919 6.264 6.895 7.231 6.997 6.772 6.513 6.336 6.869 6.558 5.714 5.848 6.219 5.747 5.851 6.015 6.266 6.491 GROUP: FEMALE 7 - 300 PPM C91491 C91492 C91493 C91494 C91495 C91496 C91497 C91498 C91499 C91500 C91501 C91502 C91503 C91504 29.105 SPILLED SPILLED 27.956 29.250 31.144 28.325 27.285 28.571 26.859 24.457 29.845 29.602 SPILLED 27.016 41.362 26.120 23.422 24.048 24.917 24.654 31.334 28.992 23.417 20.086 24.943 26.708 SPILLED 22.390 23.861 SPILLED 20.657 20.752 21.193 22.022 20.888 21.309 19.748 21.654 23.786 21.625 SPILLED 22.294 SPILLED 21.661 20.856 20.324 21.081 21.223 22.067 19.763 19.233 18.927 22.093 21.333 SPILLED 20.368 22.771 20.207 19.319 20.450 20.922 20.532 21.255 18.189 17.143 19.334 20.833 20.702 SPILLED 19.443 21.012 18.169 17.875 18.383 18.805 19.498 26.203 17.594 17.102 SPILLED 19.349 18.418 35.588 18.455 19.986 18.697 18.929 20.229 19.190 19.576 SPILLED 17.811 16.405 17.184 19.956 20.241 25.545 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1405 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 23 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 7 - 300 PPM C91505 C91506 C91507 C91508 C91509 C91510 C91511 C91512 C91513 C91514 C91515 C91516 C91517 C91518 C91519 C91520 C91521 C91522 C91523 C91524 C91525 C91526 C91527 C91528 C91529 C91530 28.052 28.652 33.791 SPILLED 28.654 28.325 28.323 25.268 27.318 30.751 SPILLED 28.312 28.094 29.086 25.352 28.391 28.656 37.927 31.504 SPILLED 42.110 26.959 28.875 31.366 SPILLED 27.731 23.823 25.668 28.739 32.366 26.057 25.580 23.249 24.290 25.854 26.786 SPILLED 23.553 SPILLED 26.625 SPILLED 24.060 24.286 31.148 35.584 23.396 25.087 22.785 SPILLED 24.866 28.791 23.718 22.120 22.625 25.522 24.310 20.244 21.951 SPILLED 20.659 20.773 24.733 25.183 20.856 20.183 20.502 SPILLED 22.556 21.596 24.170 34.138 20.866 22.709 21.376 22.761 23.003 27.198 20.260 20.705 21.064 35.159 24.228 SPILLED 21.578 21.159 19.598 20.007 22.981 20.982 21.594 19.894 20.654 SPILLED 22.164 19.000 22.568 23.525 19.310 21.700 21.377 20.540 21.167 24.070 21.482 20.727 21.279 23.297 SPILLED SPILLED 19.262 21.012 19.780 18.956 21.728 20.346 19.979 21.128 19.887 SPILLED 20.890 21.271 26.571 25.131 21.864 20.844 20.466 SPILLED 19.048 20.472 18.606 SPILLED 19.929 22.522 SPILLED 19.160 18.609 20.810 18.455 18.176 20.316 22.523 20.119 19.022 19.048 SPILLED 19.669 19.388 20.786 22.785 20.045 20.131 19.841 21.239 21.114 21.096 18.598 18.310 19.515 21.266 SPILLED 17.992 19.666 20.408 18.066 17.225 20.709 20.061 18.634 18.336 20.069 40.378 21.024 17.184 20.143 21.951 21.096 19.749 19.691 19.963 18.526 19.506 18.480 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1406 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 24 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 3 PPM C90781 C90782 C90783 C90784 C90785 C90786 C90787 C90788 C90789 C90790 C90791 C90792 C90793 C90794 C90795 C90796 C90797 C90798 C90799 C90800 C90801 C90802 C90803 C90804 C90805 C90806 C90807 C90808 C90809 C90810 C90811 0.149 0.176 0.155 0.150 0.147 0.137 0.152 0.141 NOT TAKEN 0.152 0.155 0.144 0.146 0.136 0.152 0.166 0.138 0.141 0.142 0.135 0.152 0.137 0.141 0.138 0.144 0.150 0.132 0.149 0.147 0.147 0.150 0.144 0.146 0.139 0.142 0.147 0.135 0.147 0.138 NOT TAKEN 0.149 0.170 0.139 0.142 0.140 0.158 SPILLED 0.134 0.136 0.141 0.132 0.149 0.136 0.140 0.133 0.139 0.154 0.134 0.117 0.150 0.139 0.147 0.141 0.149 0.141 0.149 0.150 0.141 0.140 0.137 NOT TAKEN 0.144 0.150 0.142 0.137 0.147 0.152 0.189 0.132 0.137 0.139 0.138 0.152 0.141 0.138 0.134 0.147 0.148 0.136 SPILLED 0.149 0.144 0.145 0.140 0.146 0.138 0.147 0.149 0.141 0.138 0.132 NOT TAKEN 0.137 0.133 0.140 0.133 0.143 0.142 0.174 NOT TAKEN 0.139 0.137 0.127 0.146 0.130 0.137 0.130 0.145 0.147 NOT TAKEN 0.141 0.144 0.139 0.144 0.135 0.142 0.143 0.139 0.148 0.139 0.138 0.135 NOT TAKEN 0.142 0.158 0.120 0.141 0.142 0.142 0.158 NOT TAKEN 0.135 0.137 0.130 0.148 0.140 0.141 0.135 0.144 0.152 NOT TAKEN 0.138 0.140 0.136 0.139 0.125 0.140 0.140 0.140 0.138 0.124 0.133 0.135 NOT TAKEN 0.137 0.149 0.110 0.136 0.148 0.135 0.149 NOT TAKEN 0.132 0.141 0.129 0.139 0.141 0.135 0.124 0.144 0.152 NOT TAKEN 0.136 0.143 0.135 0.139 0.124 0.130 0.136 0.142 0.139 0.134 0.137 0.128 NOT TAKEN 0.143 0.148 0.144 0.126 0.138 0.128 0.141 NOT TAKEN 0.140 0.139 0.126 0.139 0.133 0.128 0.125 0.136 0.151 NOT TAKEN 0.130 0.141 0.129 0.134 0.120 0.126 0.134 0.132 0.132 0.127 0.120 0.121 NOT TAKEN 0.124 0.138 0.110 0.120 0.124 0.120 0.138 NOT TAKEN 0.130 0.126 0.121 0.133 0.130 0.120 0.116 0.130 0.147 NOT TAKEN 0.125 0.132 0.127 0.131 0.113 0.071 0.128 0.132 0.124 0.126 0.118 0.120 NOT TAKEN 0.118 0.129 0.098 0.117 0.067 0.116 0.131 NOT TAKEN 0.123 0.115 0.115 0.116 0.126 0.117 0.120 0.123 0.156 NOT TAKEN 0.114 0.127 0.104 0.121 0.114 0.120 0.130 0.132 0.124 0.133 0.121 0.118 NOT TAKEN 0.130 0.140 0.126 0.119 0.022 0.121 0.135 NOT TAKEN 0.120 0.129 0.118 0.125 0.134 0.115 0.118 0.124 0.132 NOT TAKEN 0.123 0.127 0.114 0.117 1407 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 25 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 3 PPM C90812 C90813 C90814 C90815 C90816 C90817 C90818 C90819 C90820 C90821 C90822 C90823 C90824 C90825 C90826 C90827 C90828 C90829 C90830 C90831 C90832 C90833 C90834 C90835 C90836 C90837 C90838 C90839 C90840 0.136 NOT TAKEN NOT TAKEN 0.144 0.148 NOT TAKEN 0.164 0.135 0.134 0.138 0.150 0.163 0.143 0.146 0.176 0.149 0.149 0.139 0.152 0.151 0.145 NOT TAKEN NOT TAKEN 0.160 0.154 0.147 0.146 0.144 0.143 0.132 NOT TAKEN NOT TAKEN 0.142 0.145 NOT TAKEN 0.154 0.126 0.130 0.134 0.140 0.154 0.132 0.134 0.162 0.137 0.146 0.128 0.140 0.144 0.136 NOT TAKEN NOT TAKEN 0.147 0.149 0.139 0.144 0.139 0.139 0.135 NOT TAKEN NOT TAKEN 0.139 0.141 NOT TAKEN 0.164 0.120 0.134 0.133 0.147 0.158 0.135 0.133 SPILLED 0.135 0.152 0.134 0.138 0.146 0.137 NOT TAKEN NOT TAKEN 0.140 0.149 0.144 0.143 0.138 0.133 0.133 NOT TAKEN NOT TAKEN 0.137 0.128 NOT TAKEN NOT TAKEN 0.122 0.110 0.132 0.150 0.156 0.140 0.135 SPILLED 0.132 0.154 0.137 0.139 NOT TAKEN 0.133 NOT TAKEN NOT TAKEN 0.138 NOT TAKEN 0.143 0.142 0.137 0.118 0.132 NOT TAKEN NOT TAKEN 0.138 0.140 NOT TAKEN NOT TAKEN 0.131 0.130 0.133 0.149 0.152 0.136 0.137 0.175 0.132 0.143 0.133 0.146 NOT TAKEN 0.135 NOT TAKEN NOT TAKEN 0.141 NOT TAKEN 0.135 0.147 0.130 0.140 0.134 NOT TAKEN NOT TAKEN 0.131 0.135 NOT TAKEN NOT TAKEN 0.133 0.126 0.132 0.146 0.149 0.132 0.127 0.167 0.133 0.148 0.133 0.138 NOT TAKEN 0.128 NOT TAKEN NOT TAKEN 0.144 NOT TAKEN 0.134 0.139 0.134 0.134 0.129 NOT TAKEN NOT TAKEN 0.131 0.130 NOT TAKEN NOT TAKEN 0.120 0.120 0.130 0.139 0.143 0.129 0.126 0.161 0.133 0.135 0.124 0.134 NOT TAKEN 0.126 NOT TAKEN NOT TAKEN 0.136 NOT TAKEN 0.131 0.135 0.135 0.129 0.120 NOT TAKEN NOT TAKEN 0.123 0.125 NOT TAKEN NOT TAKEN 0.121 0.112 0.119 0.133 0.130 0.124 0.115 0.148 0.122 0.128 0.125 0.126 NOT TAKEN 0.118 NOT TAKEN NOT TAKEN 0.127 NOT TAKEN 0.123 0.122 0.121 0.121 0.121 NOT TAKEN NOT TAKEN 0.114 0.121 NOT TAKEN NOT TAKEN 0.117 0.111 0.118 0.129 0.126 0.120 0.112 0.094 0.116 0.123 0.112 0.065 NOT TAKEN 0.115 NOT TAKEN NOT TAKEN 0.120 NOT TAKEN 0.117 0.070 0.080 0.110 0.125 NOT TAKEN NOT TAKEN 0.122 0.123 NOT TAKEN NOT TAKEN 0.118 0.111 0.121 0.135 0.120 0.117 0.120 SPILLED 0.120 0.118 0.115 0.125 NOT TAKEN 0.115 NOT TAKEN NOT TAKEN 0.124 NOT TAKEN 0.116 0.116 0.114 0.107 1408 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 26 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90841 C90842 C90843 C90844 C90845 C90846 C90847 C90848 C90849 C90850 C90851 C90852 C90853 C90854 C90855 C90856 C90857 C90858 C90859 C90860 C90861 C90862 C90863 C90864 C90865 C90866 C90867 C90868 C90869 C90870 C90871 1.554 1.476 1.465 SPILLED 1.446 NOT TAKEN 1.457 1.494 1.459 1.433 1.600 1.461 NOT TAKEN 1.527 1.383 1.443 1.429 1.502 1.547 1.501 1.526 1.633 1.310 1.414 1.349 NOT TAKEN 1.405 1.466 1.409 1.398 1.314 1.472 1.424 1.360 1.723 1.343 NOT TAKEN 1.366 1.511 1.385 1.364 1.427 1.466 NOT TAKEN 1.451 1.385 1.317 1.409 1.394 1.497 1.602 1.462 1.600 1.243 1.386 1.333 NOT TAKEN 1.375 1.417 1.313 1.369 1.304 1.415 1.440 1.351 1.673 1.325 NOT TAKEN 1.372 1.418 1.351 1.358 1.434 1.429 NOT TAKEN 1.496 1.441 1.342 1.429 1.408 1.540 1.601 1.487 1.619 1.268 1.431 1.351 NOT TAKEN 1.414 1.390 1.344 1.394 1.304 1.413 1.401 NOT TAKEN 1.709 1.365 NOT TAKEN 1.302 1.450 1.299 1.323 1.248 NOT TAKEN NOT TAKEN SPILLED 1.337 1.117 1.357 1.456 1.448 1.551 1.377 1.555 NOT TAKEN 1.354 1.315 NOT TAKEN 1.367 1.359 1.253 1.316 1.248 1.371 1.380 NOT TAKEN 1.683 1.330 NOT TAKEN 1.348 1.451 1.274 1.299 1.431 NOT TAKEN NOT TAKEN SPILLED 1.318 1.289 1.403 1.420 1.408 1.606 1.423 1.567 NOT TAKEN 1.385 1.343 NOT TAKEN 1.355 1.366 1.276 1.401 1.292 1.375 1.329 NOT TAKEN 1.786 1.292 NOT TAKEN 1.373 1.409 1.308 1.282 1.359 NOT TAKEN NOT TAKEN 1.494 1.311 1.283 1.402 1.449 1.389 1.536 1.453 1.562 NOT TAKEN 1.355 1.320 NOT TAKEN 1.341 1.369 1.315 1.204 1.265 1.355 1.378 NOT TAKEN 1.600 1.314 NOT TAKEN 1.397 1.337 1.238 1.282 1.406 NOT TAKEN NOT TAKEN SPILLED 1.278 1.378 1.392 1.379 1.428 1.573 1.402 1.545 NOT TAKEN 1.402 1.335 NOT TAKEN 1.319 1.335 1.278 1.306 1.245 1.367 1.309 NOT TAKEN 1.472 1.275 NOT TAKEN 1.329 1.235 1.214 1.234 1.312 NOT TAKEN NOT TAKEN 1.463 1.215 1.270 1.297 1.363 1.336 1.392 1.332 1.448 NOT TAKEN 1.301 1.293 NOT TAKEN 1.326 1.204 1.198 1.235 1.196 1.284 1.279 NOT TAKEN 0.704 1.165 NOT TAKEN 1.271 1.259 1.141 1.192 1.243 NOT TAKEN NOT TAKEN SPILLED 1.104 1.150 1.214 1.324 1.229 1.403 1.292 1.352 NOT TAKEN 1.212 1.253 NOT TAKEN 1.359 1.141 1.229 1.211 1.137 1.268 1.351 NOT TAKEN 1.412 1.134 NOT TAKEN 1.347 1.149 1.189 1.186 1.281 NOT TAKEN NOT TAKEN 1.467 1.196 1.235 1.214 1.326 1.438 1.456 1.293 1.385 NOT TAKEN 1.281 1.323 NOT TAKEN 1.355 1.166 1.234 1.232 1.205 1409 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90872 C90873 C90874 C90875 C90876 C90877 C90878 C90879 C90880 C90881 C90882 C90883 C90884 C90885 C90886 C90887 C90888 C90889 C90890 C90891 C90892 C90893 C90894 C90895 C90896 C90897 C90898 C90899 C90900 1.369 1.425 1.332 1.436 1.415 1.524 1.579 1.487 1.474 1.429 1.466 SPILLED 1.639 1.454 1.523 1.494 1.412 1.357 NOT TAKEN NOT TAKEN 1.573 1.647 1.543 1.713 SPILLED 1.397 1.465 1.519 1.454 1.340 1.322 1.337 1.409 1.300 1.495 1.598 1.419 1.399 1.395 1.374 1.710 1.558 1.373 1.415 1.380 1.399 1.272 NOT TAKEN NOT TAKEN 1.446 1.476 1.417 1.559 1.584 1.300 1.368 1.403 1.393 1.367 1.420 1.290 1.457 1.292 1.494 1.585 1.419 1.456 1.416 1.411 1.517 SPILLED 1.374 1.413 1.383 1.412 1.232 NOT TAKEN NOT TAKEN 1.452 1.455 1.422 1.654 1.653 1.328 1.326 1.513 1.414 1.388 1.446 1.215 1.370 1.209 NOT TAKEN 1.533 1.387 NOT TAKEN 1.366 1.396 SPILLED 1.519 1.367 1.379 1.402 0.688 1.239 NOT TAKEN NOT TAKEN 1.290 1.459 1.483 1.506 1.463 1.013 1.360 1.380 1.365 1.330 1.488 1.238 1.456 1.166 NOT TAKEN 1.506 1.376 NOT TAKEN 1.307 1.361 1.600 SPILLED 1.350 1.346 SPILLED 1.760 1.253 NOT TAKEN NOT TAKEN 1.482 1.441 1.407 1.487 SPILLED 1.351 1.292 1.459 1.392 1.357 1.332 1.239 1.378 1.136 NOT TAKEN 1.519 1.396 NOT TAKEN 1.345 1.376 1.559 SPILLED 1.351 1.364 1.413 1.544 1.321 NOT TAKEN NOT TAKEN 1.399 1.465 1.409 1.407 1.423 1.390 1.288 1.423 1.426 1.323 1.403 1.174 1.334 1.243 NOT TAKEN 1.523 1.359 NOT TAKEN 1.282 1.287 1.466 SPILLED 1.379 1.380 1.336 1.399 1.264 NOT TAKEN NOT TAKEN 1.372 1.439 1.348 1.364 1.514 1.334 1.378 1.394 1.398 1.289 1.295 1.136 1.265 1.200 NOT TAKEN 1.461 1.387 NOT TAKEN 1.280 1.299 1.494 1.597 1.239 1.259 1.296 1.268 1.215 NOT TAKEN NOT TAKEN 1.276 1.329 1.309 1.297 1.399 1.221 1.292 1.422 1.310 1.308 1.320 1.104 1.144 1.116 NOT TAKEN 1.413 1.263 NOT TAKEN 1.208 1.253 1.414 1.498 1.159 1.222 1.182 1.275 1.185 NOT TAKEN NOT TAKEN 1.247 1.263 1.209 1.231 1.301 1.117 1.180 1.278 1.263 1.198 1.269 1.082 1.201 1.086 NOT TAKEN 1.348 1.231 NOT TAKEN 1.163 1.223 1.332 1.454 1.201 1.289 1.254 1.350 1.273 NOT TAKEN NOT TAKEN 1.272 1.346 1.275 1.312 1.389 1.183 1.241 1.379 1.304 1410 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 28 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90901 C90902 C90903 C90904 C90905 C90906 C90907 C90908 C90909 C90910 C90911 C90912 C90913 C90914 C90915 C90916 C90917 C90918 C90919 C90920 C90921 C90922 C90923 C90924 C90925 C90926 C90927 C90928 C90929 C90930 C90931 4.794 4.715 4.746 4.863 5.119 5.017 4.632 4.641 4.821 5.065 4.980 4.531 5.262 SPILLED 4.885 4.816 4.660 4.762 4.682 4.675 4.653 4.584 4.905 5.190 4.984 4.521 4.606 4.478 4.877 5.216 4.659 4.757 4.660 4.538 4.492 5.057 4.836 4.361 4.666 4.696 4.689 4.617 4.380 4.709 SPILLED 4.727 4.594 4.581 4.731 4.613 4.559 4.528 4.491 4.673 4.934 4.855 4.538 4.492 4.405 4.643 5.239 4.391 4.695 4.767 4.589 4.703 5.046 4.721 4.542 4.722 4.594 4.906 4.836 4.496 4.551 4.853 4.617 4.648 4.587 4.732 4.482 4.632 4.364 4.625 4.623 4.915 4.803 4.329 4.565 4.612 3.509 5.072 4.537 4.855 4.644 4.429 4.577 NOT TAKEN 4.739 NOT TAKEN 4.613 4.570 4.752 4.803 4.374 5.064 4.611 4.796 4.711 NOT TAKEN 4.613 4.510 4.452 NOT TAKEN 4.589 4.649 4.875 4.827 4.444 4.481 4.535 5.079 4.942 4.482 4.970 4.613 4.526 4.507 NOT TAKEN 4.744 NOT TAKEN 4.762 4.474 4.846 4.771 4.281 4.847 4.529 4.783 4.757 NOT TAKEN 4.556 4.421 4.325 NOT TAKEN 4.468 4.646 4.534 4.836 4.413 4.472 4.499 5.137 4.917 4.424 4.787 4.541 4.627 4.422 NOT TAKEN 4.661 NOT TAKEN 4.573 4.441 4.721 4.673 4.282 5.030 4.465 4.660 4.619 NOT TAKEN 4.309 4.552 4.465 NOT TAKEN 4.513 4.531 4.597 4.781 4.516 4.454 4.619 4.916 4.766 4.402 4.936 4.520 4.378 4.480 NOT TAKEN 4.668 NOT TAKEN 4.505 4.764 4.756 4.738 4.624 5.143 4.582 4.401 4.590 NOT TAKEN 4.520 4.516 4.569 NOT TAKEN 4.622 4.673 4.681 4.682 4.399 4.392 4.508 4.666 4.489 4.419 4.589 4.268 4.310 4.521 NOT TAKEN 4.543 NOT TAKEN 4.423 4.470 4.572 4.440 4.237 4.774 4.373 4.377 4.499 NOT TAKEN 4.163 4.356 4.222 NOT TAKEN 4.360 4.387 4.220 4.411 4.170 4.326 4.376 4.391 4.521 4.266 4.527 4.183 4.030 4.170 NOT TAKEN 4.445 NOT TAKEN 4.293 4.000 4.284 4.259 3.670 4.687 4.128 4.461 4.530 NOT TAKEN 4.153 4.188 4.124 NOT TAKEN 4.225 4.440 4.181 4.589 3.964 4.038 4.102 4.485 4.473 4.100 4.522 4.201 3.898 4.232 NOT TAKEN 4.524 NOT TAKEN 3.992 3.919 4.283 4.263 4.283 4.357 4.101 4.241 4.487 NOT TAKEN 4.317 3.934 4.076 NOT TAKEN 4.217 4.636 3.786 4.472 4.207 5.270 4.130 4.547 4.424 4.237 1411 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 29 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90932 C90933 C90934 C90935 C90936 C90937 C90938 C90939 C90940 C90941 C90942 C90943 C90944 C90945 C90946 C90947 C90948 C90949 C90950 C90951 C90952 C90953 C90954 C90955 C90956 C90957 C90958 C90959 C90960 C90961 C90962 4.922 4.594 5.114 NOT TAKEN 5.040 4.747 5.192 NOT TAKEN 4.850 4.802 NOT TAKEN 5.442 4.668 4.725 4.020 4.909 4.914 3.327 4.689 4.853 4.492 4.603 4.851 4.776 4.206 5.223 4.656 4.082 4.738 NOT TAKEN 4.908 4.907 4.575 4.881 NOT TAKEN 5.014 4.611 4.929 NOT TAKEN 4.659 4.478 NOT TAKEN 5.815 4.680 4.710 4.369 4.697 4.946 2.752 4.520 4.729 4.219 4.535 4.643 4.455 4.154 4.524 3.690 4.429 4.625 NOT TAKEN 4.666 4.936 4.457 4.865 NOT TAKEN 4.932 4.641 4.927 NOT TAKEN 4.615 4.630 NOT TAKEN SPILLED 4.569 4.494 4.623 4.703 5.002 NOT TAKEN 4.582 4.688 4.185 4.393 4.700 4.501 4.042 4.817 5.119 4.513 4.832 NOT TAKEN 4.633 4.959 4.656 4.450 NOT TAKEN 4.877 4.782 4.706 NOT TAKEN 4.482 4.382 NOT TAKEN 6.394 4.410 4.587 4.436 4.438 5.025 NOT TAKEN 4.314 4.744 4.068 3.850 5.021 4.487 4.438 4.469 4.925 4.434 NOT TAKEN NOT TAKEN 4.548 4.977 4.658 5.154 NOT TAKEN 4.795 4.627 4.970 NOT TAKEN 4.465 4.437 NOT TAKEN SPILLED 4.656 4.541 4.498 4.757 5.040 NOT TAKEN 4.459 4.469 4.017 4.501 4.827 4.635 4.394 4.938 4.807 4.626 NOT TAKEN NOT TAKEN 4.585 4.615 4.464 4.885 NOT TAKEN 4.670 3.952 4.961 NOT TAKEN 4.367 4.539 NOT TAKEN 5.420 4.539 4.456 4.420 4.832 4.936 NOT TAKEN 4.543 4.624 4.335 4.362 4.708 4.490 4.351 4.771 4.965 4.570 NOT TAKEN NOT TAKEN 4.474 4.724 4.505 4.642 NOT TAKEN 3.223 5.208 4.904 NOT TAKEN 4.507 4.727 NOT TAKEN SPILLED 4.527 4.474 4.659 4.730 5.039 NOT TAKEN 4.410 4.472 4.272 4.223 4.642 4.432 4.454 2.989 4.763 4.572 NOT TAKEN NOT TAKEN 4.297 4.838 4.295 4.645 NOT TAKEN 4.758 4.983 4.911 NOT TAKEN 4.413 4.761 NOT TAKEN 5.399 4.462 4.383 4.389 4.552 4.921 NOT TAKEN 4.282 4.236 3.890 4.079 4.502 4.502 4.032 4.279 SPILLED 4.135 NOT TAKEN NOT TAKEN 4.268 4.627 4.015 4.481 NOT TAKEN 4.303 5.187 4.756 NOT TAKEN 4.427 4.295 NOT TAKEN 5.027 4.454 4.420 4.001 4.240 4.564 NOT TAKEN 4.178 4.200 3.845 4.030 4.399 4.393 4.053 4.389 4.274 4.157 NOT TAKEN NOT TAKEN 4.077 4.887 4.049 4.810 NOT TAKEN 4.506 4.912 4.809 NOT TAKEN 4.138 4.259 NOT TAKEN SPILLED 4.386 4.514 3.728 4.426 4.226 NOT TAKEN 4.283 4.220 3.974 4.309 4.659 4.364 3.788 4.656 4.312 4.095 NOT TAKEN NOT TAKEN 4.177 1412 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 30 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90963 C90964 C90965 C90966 C90967 C90968 C90969 C90970 4.491 4.466 4.864 NOT TAKEN 4.762 4.847 5.241 5.062 4.473 4.439 4.823 NOT TAKEN 4.505 4.752 5.055 4.731 4.490 4.295 2.777 NOT TAKEN 4.624 4.687 5.052 4.802 4.599 4.285 5.388 NOT TAKEN 4.544 4.238 4.964 4.702 4.485 4.413 SPILLED NOT TAKEN 4.542 4.692 4.700 4.786 4.386 4.449 5.405 NOT TAKEN 4.381 4.671 4.719 4.713 4.479 4.508 SPILLED NOT TAKEN 4.297 4.608 4.813 4.723 4.248 4.284 5.482 NOT TAKEN 4.143 4.387 4.636 4.296 4.309 4.054 4.612 NOT TAKEN 4.185 4.022 4.806 4.173 3.998 3.909 SPILLED NOT TAKEN 4.246 4.263 4.557 3.973 GROUP: C91041 C91042 C91043 C91044 C91045 C91046 C91047 C91048 C91049 MALE 6 - 100 PPM 4.766 5.147 5.396 5.409 4.744 NOT TAKEN 5.198 5.197 5.053 4.497 5.143 5.073 5.140 4.736 NOT TAKEN 5.172 4.999 4.745 4.493 5.171 5.161 5.105 4.571 NOT TAKEN 4.855 5.154 4.745 4.340 4.757 4.926 4.958 4.464 NOT TAKEN 5.108 4.689 4.643 4.443 4.781 4.808 4.983 4.313 NOT TAKEN 4.941 4.789 4.766 4.390 4.945 4.900 4.912 4.472 NOT TAKEN 4.636 5.025 4.704 4.234 4.823 4.856 4.939 4.323 NOT TAKEN 4.745 4.837 4.700 4.127 4.512 4.889 4.960 4.222 NOT TAKEN 4.896 4.774 4.602 4.039 4.553 4.619 4.776 4.102 NOT TAKEN 4.571 4.366 4.041 3.998 4.468 4.443 4.731 3.969 NOT TAKEN 4.017 4.222 4.182 1413 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 31 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 100 PPM C91050 C91051 C91052 C91053 C91054 C91055 C91056 C91057 C91058 C91059 C91060 C91061 C91062 C91063 C91064 C91065 C91066 C91067 C91068 C91069 C91070 C91071 C91072 C91073 C91074 C91075 C91076 C91077 C91078 C91079 C91080 5.270 5.171 4.966 4.843 4.539 4.821 5.574 4.565 4.216 4.907 5.391 4.748 4.929 4.714 4.661 4.880 4.711 4.875 3.853 5.518 4.657 4.912 4.865 5.015 4.815 5.359 5.192 4.986 4.464 4.813 4.977 5.091 4.970 4.730 4.797 4.415 4.535 5.492 4.552 4.086 4.744 4.979 4.811 4.706 5.038 4.631 4.752 4.640 4.720 NOT TAKEN 5.177 4.448 4.664 4.644 4.754 4.652 5.146 5.066 4.801 4.375 4.620 4.843 4.952 4.979 SPILLED 4.840 4.542 4.519 5.345 4.369 4.291 4.671 5.172 4.892 4.771 4.958 4.607 4.978 4.691 4.726 NOT TAKEN 4.857 4.360 4.810 4.863 4.697 4.855 5.245 4.752 4.868 4.409 4.648 4.984 4.809 4.833 4.433 4.824 4.326 4.626 5.552 4.412 4.358 4.624 5.070 4.779 4.717 4.808 4.726 4.796 4.653 4.552 NOT TAKEN 4.708 4.484 4.599 4.667 4.726 4.559 4.858 4.785 4.953 4.355 4.680 4.781 4.880 4.774 4.668 4.805 4.418 4.723 5.359 4.427 4.372 4.528 5.228 4.532 4.655 4.779 4.692 4.844 4.543 4.546 NOT TAKEN 4.740 4.668 4.648 4.653 4.460 4.495 4.989 4.744 5.002 4.169 4.506 4.701 5.012 4.676 4.511 4.762 4.902 4.424 5.222 4.510 4.203 4.311 5.069 4.544 4.718 4.695 4.637 4.734 4.366 4.537 NOT TAKEN 4.671 4.507 4.408 4.614 4.540 4.587 4.831 4.523 4.867 4.484 4.638 4.633 5.101 4.810 4.519 4.645 4.668 4.424 4.783 4.205 4.126 4.409 5.103 4.449 4.482 4.767 4.395 4.388 4.429 4.350 NOT TAKEN 4.647 4.339 4.311 4.398 4.039 4.633 4.912 4.931 4.640 4.459 4.686 4.776 4.612 4.661 4.122 4.491 4.300 4.186 4.836 4.152 4.125 4.340 4.663 4.415 4.471 4.367 4.387 4.507 4.364 4.352 NOT TAKEN 4.191 4.462 4.297 4.371 4.133 4.389 4.818 4.347 4.405 4.022 4.290 4.601 4.460 4.413 3.786 4.292 3.863 4.070 SPILLED 3.852 3.938 4.270 4.427 4.020 4.463 4.434 4.201 4.326 4.109 4.272 NOT TAKEN 4.213 4.159 4.221 4.229 3.856 4.282 4.520 4.253 4.683 3.935 4.285 4.318 4.565 4.509 3.911 4.165 4.195 3.980 SPILLED 4.003 4.182 4.043 4.387 4.062 SPILLED 4.663 4.109 3.979 4.175 4.394 NOT TAKEN 4.249 4.561 4.266 4.157 3.736 4.454 SPILLED 4.257 4.837 4.198 4.163 4.375 1414 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 32 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91191 C91192 C91193 C91194 C91195 C91196 C91197 C91198 C91199 C91200 C91201 C91202 C91203 C91204 C91205 C91206 C91207 C91208 C91209 0.157 0.194 SPILLED 0.192 0.180 0.177 0.211 NOT TAKEN SPILLED 0.290 SPILLED 0.165 0.172 0.217 NOT TAKEN 0.185 0.192 0.181 0.159 0.163 0.183 SPILLED 0.183 0.168 0.177 0.200 NOT TAKEN 0.221 0.201 0.205 0.147 0.164 0.208 NOT TAKEN 0.163 0.180 0.184 0.158 0.147 0.183 SPILLED 0.182 0.172 0.158 SPILLED NOT TAKEN SPILLED 0.206 0.226 0.149 0.167 0.193 NOT TAKEN 0.162 0.190 0.165 0.163 0.170 NOT TAKEN 0.201 0.189 0.170 0.151 0.201 NOT TAKEN 0.290 0.188 0.196 0.150 0.174 0.168 NOT TAKEN 0.178 0.174 0.184 0.178 0.163 NOT TAKEN 0.194 0.172 0.170 0.169 SPILLED NOT TAKEN 0.330 0.187 0.176 0.164 0.176 0.204 NOT TAKEN 0.177 0.179 SPILLED 0.162 0.144 NOT TAKEN SPILLED 0.177 0.157 0.166 0.192 NOT TAKEN 0.219 SPILLED 0.171 0.157 0.171 0.194 NOT TAKEN 0.166 0.176 0.180 0.171 0.180 NOT TAKEN 0.237 0.175 0.188 0.162 0.185 NOT TAKEN SPILLED 0.189 0.204 0.137 0.158 0.182 NOT TAKEN 0.181 0.171 SPILLED 0.151 0.160 NOT TAKEN 0.208 0.177 0.180 0.167 0.193 NOT TAKEN 0.168 0.171 0.176 0.147 0.155 0.163 NOT TAKEN 0.186 0.172 0.178 0.166 0.154 NOT TAKEN SPILLED 0.169 0.175 0.153 0.172 NOT TAKEN 0.168 0.176 0.188 0.147 0.139 0.147 NOT TAKEN 0.170 0.175 0.183 0.170 0.146 NOT TAKEN 0.170 0.161 0.171 0.160 0.178 NOT TAKEN 0.179 0.164 0.167 0.139 0.143 0.166 NOT TAKEN 0.161 0.171 0.170 0.183 1415 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 33 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91210 C91211 C91212 C91213 C91214 C91215 C91216 C91217 C91218 C91219 C91220 C91221 C91222 C91223 C91224 C91225 C91226 C91227 C91228 C91229 C91230 C91231 C91232 C91233 C91234 C91235 C91236 C91237 C91238 C91239 C91240 SPILLED 0.157 0.188 0.198 0.183 0.183 0.248 0.193 SPILLED SPILLED 0.172 0.187 NOT TAKEN 0.186 0.236 0.176 0.184 0.179 0.184 0.168 NOT TAKEN 0.163 0.203 0.195 0.171 SPILLED 0.198 NOT TAKEN SPILLED 0.180 0.143 SPILLED 0.170 0.180 0.169 0.149 0.191 SPILLED 0.185 SPILLED SPILLED 0.161 0.205 NOT TAKEN 0.207 0.202 0.170 0.179 0.172 0.180 0.170 NOT TAKEN 0.153 0.213 0.190 0.169 SPILLED 0.192 NOT TAKEN 0.265 0.173 0.135 0.323 0.161 0.183 0.176 0.161 0.185 0.187 0.172 SPILLED SPILLED 0.151 0.192 NOT TAKEN 0.199 0.191 0.169 0.187 0.161 0.186 0.175 NOT TAKEN 0.156 0.192 0.173 0.173 SPILLED 0.183 NOT TAKEN 0.172 0.175 0.181 0.277 0.164 0.174 0.192 0.167 0.175 0.189 0.171 SPILLED SPILLED 0.154 0.161 NOT TAKEN NOT TAKEN 0.192 0.172 0.172 0.159 0.148 0.159 NOT TAKEN 0.159 0.179 NOT TAKEN 0.157 SPILLED 0.192 NOT TAKEN 0.171 0.172 0.185 SPILLED 0.161 0.164 0.186 0.179 0.189 0.200 0.178 SPILLED SPILLED 0.162 0.193 NOT TAKEN NOT TAKEN 0.186 0.157 0.179 0.165 0.175 0.158 NOT TAKEN 0.162 SPILLED NOT TAKEN 0.161 SPILLED 0.189 NOT TAKEN 0.173 0.178 0.161 SPILLED 0.163 0.168 0.174 0.173 0.200 0.177 0.167 SPILLED SPILLED 0.154 0.187 NOT TAKEN NOT TAKEN 0.192 0.170 0.178 0.152 0.182 0.161 NOT TAKEN 0.182 0.196 NOT TAKEN 0.162 SPILLED 0.196 NOT TAKEN 0.184 0.158 0.168 SPILLED 0.164 0.179 0.173 0.156 0.190 0.168 0.181 SPILLED SPILLED 0.152 0.203 NOT TAKEN NOT TAKEN 0.182 0.143 0.178 0.149 0.164 0.178 NOT TAKEN 0.146 0.201 NOT TAKEN 0.152 0.292 0.203 NOT TAKEN 0.168 0.180 0.158 SPILLED 0.156 0.171 0.177 0.179 0.196 0.180 0.166 0.202 SPILLED 0.165 0.174 NOT TAKEN NOT TAKEN 0.185 0.186 0.168 0.143 0.180 0.162 NOT TAKEN 0.135 0.182 NOT TAKEN 0.143 0.240 0.228 NOT TAKEN 0.180 0.163 0.197 0.229 0.173 0.164 0.128 0.148 0.188 0.183 0.177 0.192 0.217 0.146 0.156 NOT TAKEN NOT TAKEN 0.188 0.147 0.157 0.149 0.171 0.162 NOT TAKEN 0.142 0.170 NOT TAKEN 0.134 0.249 0.213 NOT TAKEN 0.165 0.167 0.149 0.223 0.137 0.168 0.160 0.147 0.200 0.178 0.177 SPILLED 0.177 0.157 0.178 NOT TAKEN NOT TAKEN 0.198 0.161 0.154 0.148 0.165 0.161 NOT TAKEN 0.145 0.171 NOT TAKEN 0.153 0.212 0.228 NOT TAKEN 0.184 0.167 0.154 1416 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 34 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91241 C91242 C91243 C91244 C91245 C91246 C91247 C91248 C91249 C91250 SPILLED 0.340 0.241 0.184 0.224 SPILLED SPILLED 0.181 0.193 0.189 0.176 SPILLED SPILLED 0.171 SPILLED SPILLED SPILLED SPILLED 0.176 0.177 0.177 SPILLED 0.210 0.168 0.209 SPILLED 0.273 0.157 0.169 0.240 NOT TAKEN SPILLED 0.264 0.172 0.206 SPILLED 0.233 NOT TAKEN 0.161 0.172 NOT TAKEN SPILLED SPILLED 0.174 SPILLED SPILLED SPILLED NOT TAKEN 0.172 0.178 NOT TAKEN SPILLED 0.217 0.175 0.200 SPILLED 0.222 NOT TAKEN 0.169 0.178 NOT TAKEN SPILLED SPILLED SPILLED 0.179 SPILLED 0.212 NOT TAKEN 0.170 0.168 NOT TAKEN 0.321 0.225 0.179 0.189 0.325 SPILLED NOT TAKEN 0.159 0.161 NOT TAKEN SPILLED 0.224 0.180 0.155 SPILLED SPILLED NOT TAKEN 0.157 0.160 NOT TAKEN 0.308 0.191 0.182 0.150 SPILLED 0.190 NOT TAKEN 0.150 0.161 GROUP: FEMALE 3 - 30 PPM C91251 C91252 C91253 C91254 C91255 C91256 C91257 C91258 C91259 C91260 C91261 C91262 C91263 C91264 C91265 C91266 C91267 C91268 NOT TAKEN 1.879 1.921 2.061 2.193 1.967 SPILLED 1.865 SPILLED 1.930 2.003 1.768 1.945 1.665 1.705 1.915 1.974 1.832 NOT TAKEN 1.725 1.967 1.819 2.026 1.922 SPILLED 1.720 1.812 1.676 2.080 1.839 1.848 1.862 1.957 1.829 1.806 1.760 NOT TAKEN 1.690 1.973 1.844 1.995 1.832 2.566 1.667 1.703 1.635 1.962 1.763 1.829 1.661 1.608 1.642 1.788 1.639 NOT TAKEN 1.888 1.780 1.775 1.810 1.754 SPILLED 1.633 1.752 1.777 1.931 1.647 1.838 1.639 1.663 1.749 1.874 1.555 NOT TAKEN 1.792 1.940 1.892 1.826 1.785 2.356 1.784 1.777 1.912 1.958 1.929 1.879 1.922 1.726 1.741 1.786 1.711 NOT TAKEN 1.792 1.829 1.812 1.895 1.781 2.107 1.595 1.787 1.754 2.096 1.773 1.869 1.702 1.650 1.685 1.875 1.622 NOT TAKEN 1.845 1.935 1.691 1.767 1.876 1.506 1.775 1.798 1.781 1.996 1.971 1.878 1.847 1.573 1.765 1.785 1.559 NOT TAKEN 1.594 1.731 1.618 1.760 1.702 SPILLED 1.559 1.732 1.723 2.002 1.856 1.729 1.577 1.555 2.109 1.904 1.651 NOT TAKEN 1.718 1.767 1.750 1.850 1.620 SPILLED 1.684 1.761 1.574 1.818 1.802 1.707 1.689 1.555 1.771 SPILLED 1.505 NOT TAKEN 1.649 1.662 1.736 1.826 1.583 SPILLED 1.535 1.621 1.679 1.833 1.655 1.666 1.701 1.625 1.862 1.777 1.549 1417 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 35 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 30 PPM C91269 C91270 C91271 C91272 C91273 C91274 C91275 C91276 C91277 C91278 C91279 C91280 C91281 C91282 C91283 C91284 C91285 C91286 C91287 C91288 C91289 C91290 C91291 C91292 C91293 C91294 C91295 C91296 C91297 C91298 C91299 2.042 2.166 2.819 NOT TAKEN 2.402 1.941 SPILLED 2.134 1.919 SPILLED 2.013 1.773 SPILLED 1.853 1.809 1.947 NOT TAKEN 2.252 NOT TAKEN 1.616 2.033 2.002 NOT TAKEN 1.797 2.641 1.730 2.147 SPILLED 2.202 1.702 1.876 2.097 1.739 2.078 NOT TAKEN 2.379 1.846 1.821 1.935 1.772 1.765 1.996 1.993 SPILLED 1.900 1.670 SPILLED NOT TAKEN 2.297 NOT TAKEN 1.680 1.780 1.926 NOT TAKEN 1.761 1.960 1.804 2.126 2.984 2.328 2.101 2.423 2.036 1.740 2.001 NOT TAKEN 2.357 1.781 1.655 1.714 1.790 1.723 1.770 1.891 2.546 1.813 1.638 2.094 NOT TAKEN 2.171 NOT TAKEN 1.620 1.675 1.775 NOT TAKEN 1.637 1.887 1.772 1.874 2.165 2.012 2.120 1.782 1.786 1.972 1.992 NOT TAKEN 2.334 1.984 1.996 1.897 1.836 1.810 1.978 1.865 NOT TAKEN 1.894 1.705 SPILLED NOT TAKEN 2.049 NOT TAKEN NOT TAKEN 1.822 2.125 NOT TAKEN 1.695 NOT TAKEN 1.738 2.114 2.096 SPILLED SPILLED NOT TAKEN 1.860 1.908 1.961 NOT TAKEN SPILLED SPILLED 1.777 1.999 1.733 1.770 1.918 1.947 NOT TAKEN 1.784 1.554 1.967 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 1.782 2.213 NOT TAKEN 1.794 NOT TAKEN 1.774 SPILLED SPILLED 2.521 SPILLED NOT TAKEN SPILLED 1.934 1.872 NOT TAKEN SPILLED 1.920 1.725 1.859 1.717 1.717 1.919 1.803 NOT TAKEN 1.795 1.667 1.903 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 1.730 2.204 NOT TAKEN 1.733 NOT TAKEN 1.697 1.988 SPILLED 2.061 SPILLED NOT TAKEN 1.776 1.849 1.883 NOT TAKEN 2.103 1.737 1.672 1.892 1.620 1.835 2.168 1.896 NOT TAKEN 1.706 1.681 2.108 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 1.817 2.214 NOT TAKEN 1.704 NOT TAKEN 1.700 1.899 2.038 2.196 SPILLED NOT TAKEN 1.556 1.734 1.899 NOT TAKEN 1.926 1.771 1.774 1.784 1.707 1.803 1.663 1.848 NOT TAKEN 1.580 1.647 1.931 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 1.772 2.273 NOT TAKEN 1.470 NOT TAKEN 1.611 1.655 1.872 2.219 SPILLED NOT TAKEN 1.760 1.651 1.659 NOT TAKEN 1.795 1.606 1.546 1.899 1.737 1.737 1.603 1.746 NOT TAKEN 1.676 1.619 1.831 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 1.822 1.583 NOT TAKEN 1.544 NOT TAKEN 1.681 1.873 1.886 SPILLED 1.639 NOT TAKEN 1.606 1.767 1.770 NOT TAKEN 1.813 1.617 1.544 1.938 1.770 1.751 1.653 1.674 NOT TAKEN 1.545 1.497 1.581 NOT TAKEN 2.167 NOT TAKEN NOT TAKEN 1.739 2.387 NOT TAKEN 1.357 NOT TAKEN 1.869 1.642 1.874 2.425 SPILLED NOT TAKEN 1418 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 36 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 30 PPM C91300 C91301 C91302 C91303 C91304 C91305 C91306 C91307 C91308 C91309 C91310 3.423 1.799 2.701 1.655 1.914 2.030 1.838 1.726 2.657 1.658 1.937 2.049 1.857 SPILLED 1.804 1.979 2.030 2.015 1.708 2.496 1.736 2.047 2.132 1.821 SPILLED 1.521 1.893 1.958 1.947 1.573 3.042 1.770 1.962 2.061 1.754 1.785 1.679 NOT TAKEN 1.803 1.840 1.743 SPILLED 1.758 1.888 SPILLED 1.811 1.688 1.729 NOT TAKEN 2.096 1.986 1.609 SPILLED 1.848 1.912 SPILLED 1.732 1.743 1.568 NOT TAKEN 2.059 1.823 1.618 2.899 1.770 1.913 SPILLED 1.777 1.721 1.686 NOT TAKEN 2.008 1.855 1.732 2.450 1.749 1.828 2.113 1.780 1.842 1.483 NOT TAKEN 1.903 1.807 1.732 2.311 1.691 1.821 1.818 1.484 1.755 1.365 NOT TAKEN 1.861 1.768 1.559 SPILLED 1.618 1.637 1.758 1.460 1.810 1.618 NOT TAKEN 1.875 2.017 1.669 2.042 1.679 1.618 GROUP: FEMALE 4 - 100 PPM C91311 C91312 C91313 C91314 C91315 C91316 C91317 C91318 C91319 C91320 C91321 C91322 C91323 C91324 C91325 C91326 C91327 6.200 6.233 7.943 8.759 7.427 NOT TAKEN NOT TAKEN 5.470 6.932 6.368 6.751 6.713 6.524 6.452 5.572 7.058 6.324 6.839 6.158 6.270 SPILLED 6.698 NOT TAKEN NOT TAKEN 5.679 6.466 6.419 6.872 6.713 6.619 8.698 5.445 6.746 5.680 7.926 6.153 6.367 SPILLED 6.657 NOT TAKEN NOT TAKEN 5.226 6.379 6.118 6.092 6.635 6.602 5.703 5.291 6.057 6.081 6.696 6.157 6.392 SPILLED 6.448 NOT TAKEN NOT TAKEN 5.495 6.313 6.572 6.836 6.419 6.851 6.127 5.641 6.083 5.974 6.652 6.237 6.557 SPILLED 7.143 NOT TAKEN NOT TAKEN NOT TAKEN 6.540 6.398 6.775 6.661 6.726 6.045 5.527 7.522 6.090 7.034 6.101 6.197 SPILLED 6.699 NOT TAKEN NOT TAKEN NOT TAKEN 6.343 5.952 6.204 6.991 6.641 6.062 5.673 6.536 6.138 7.439 6.027 6.501 SPILLED 6.610 NOT TAKEN NOT TAKEN NOT TAKEN 6.452 6.507 6.759 6.439 7.180 5.922 5.560 7.151 6.320 7.002 5.882 6.561 SPILLED 6.433 NOT TAKEN NOT TAKEN NOT TAKEN 6.230 SPILLED 6.928 NOT TAKEN 6.773 6.112 5.518 7.212 5.884 7.264 6.096 6.574 SPILLED 6.413 NOT TAKEN NOT TAKEN NOT TAKEN 6.408 6.036 6.776 NOT TAKEN 7.051 5.709 5.391 7.050 5.707 7.050 6.173 6.919 SPILLED 6.535 NOT TAKEN NOT TAKEN NOT TAKEN 6.239 5.852 6.938 NOT TAKEN 6.976 5.833 5.693 6.948 5.860 1419 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 37 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91328 C91329 C91330 C91331 C91332 C91333 C91334 C91335 C91336 C91337 C91338 C91339 C91340 C91341 C91342 C91343 C91344 C91345 C91346 C91347 C91348 C91349 C91350 C91351 C91352 C91353 C91354 C91355 C91356 C91357 C91358 5.945 5.906 6.135 6.538 7.249 NOT TAKEN 5.811 6.164 SPILLED 5.714 6.156 5.835 5.544 5.766 6.067 6.726 5.757 NOT TAKEN 6.267 5.736 5.822 6.032 NOT TAKEN 6.052 5.714 6.349 5.690 8.006 6.744 6.076 6.551 6.150 5.626 5.931 5.766 7.309 NOT TAKEN 6.032 5.874 SPILLED 5.871 5.701 6.161 5.351 5.530 6.084 6.524 5.532 NOT TAKEN 6.445 6.095 5.870 6.015 NOT TAKEN 5.677 5.284 6.517 5.691 7.027 6.137 5.357 5.775 5.229 5.592 5.838 5.929 6.589 NOT TAKEN 5.673 5.919 9.446 5.481 6.061 5.563 5.042 5.338 5.511 6.436 5.442 NOT TAKEN 6.149 5.640 5.726 6.134 NOT TAKEN 5.585 5.578 6.272 5.386 5.876 5.881 5.361 6.036 5.843 NOT TAKEN 5.904 6.148 5.547 NOT TAKEN 5.901 6.187 SPILLED NOT TAKEN 6.019 5.811 5.169 5.592 5.747 6.423 6.138 NOT TAKEN 6.417 5.788 5.353 6.397 NOT TAKEN 5.933 5.590 6.672 5.726 NOT TAKEN 5.872 5.263 5.874 5.970 NOT TAKEN 6.349 6.305 SPILLED NOT TAKEN 5.632 5.781 SPILLED NOT TAKEN 6.342 6.088 5.112 5.664 5.671 6.556 5.832 NOT TAKEN 6.318 5.926 6.110 6.501 NOT TAKEN 5.542 6.055 6.243 5.536 NOT TAKEN 6.448 5.463 6.239 5.492 NOT TAKEN 5.885 6.136 6.362 NOT TAKEN 5.541 6.024 SPILLED NOT TAKEN 6.349 5.809 5.438 5.454 6.040 5.909 5.799 NOT TAKEN 6.674 6.216 6.148 6.284 NOT TAKEN 5.982 5.937 6.299 5.996 NOT TAKEN 6.193 5.478 6.344 SPILLED NOT TAKEN 6.229 6.015 6.157 NOT TAKEN 5.544 6.066 SPILLED NOT TAKEN 6.009 6.106 5.272 5.830 5.840 6.689 5.508 NOT TAKEN 6.286 5.534 5.874 6.269 NOT TAKEN 5.497 6.149 6.505 SPILLED NOT TAKEN 7.026 5.741 6.139 6.510 NOT TAKEN 6.018 5.855 5.951 NOT TAKEN 5.774 5.685 SPILLED NOT TAKEN 5.726 5.923 5.979 6.287 5.942 6.555 5.740 NOT TAKEN 5.967 5.932 5.778 6.493 NOT TAKEN 5.652 5.690 6.729 5.769 NOT TAKEN 6.813 5.082 5.926 5.629 NOT TAKEN 5.644 6.061 5.665 NOT TAKEN 5.542 5.479 SPILLED NOT TAKEN 5.332 6.204 5.081 5.982 5.340 6.525 5.640 NOT TAKEN 6.139 5.676 5.905 7.411 NOT TAKEN 5.175 5.674 6.453 5.794 NOT TAKEN 6.439 5.412 5.500 5.281 NOT TAKEN 6.067 5.581 7.561 NOT TAKEN 5.577 5.456 10.100 NOT TAKEN 5.568 6.258 5.486 5.891 5.654 6.277 5.768 NOT TAKEN 5.793 5.814 6.089 7.023 NOT TAKEN 5.839 5.960 6.566 5.907 NOT TAKEN SPILLED 5.373 6.236 1420 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 38 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91359 C91360 C91361 C91362 C91363 C91364 C91365 C91366 C91367 C91368 C91369 C91370 C91371 C91372 C91373 C91374 C91375 C91376 C91377 C91378 C91379 C91380 SPILLED 6.356 4.595 7.673 6.015 5.918 15.524 6.092 6.023 5.886 6.314 6.730 SPILLED 6.195 5.833 5.662 6.122 5.907 7.072 6.624 6.509 6.161 8.246 6.140 5.347 8.148 6.186 5.808 SPILLED 5.370 5.549 6.090 5.821 6.701 7.742 6.291 5.522 5.942 6.091 5.703 6.945 5.691 6.971 6.273 10.138 6.122 5.408 6.253 5.964 5.528 14.975 5.497 5.510 5.737 6.525 6.659 7.046 5.892 6.356 5.459 6.228 5.501 6.343 5.657 6.646 5.375 7.745 6.144 5.236 SPILLED 6.238 5.864 14.839 6.191 6.074 5.378 6.126 NOT TAKEN 5.762 5.577 6.291 4.838 5.993 5.714 6.209 6.343 NOT TAKEN 6.040 7.664 6.283 5.367 5.850 5.970 5.361 SPILLED 5.563 5.666 5.692 6.090 NOT TAKEN 5.950 5.944 6.204 6.010 5.874 5.825 7.033 6.255 NOT TAKEN 6.000 6.146 6.077 5.107 5.693 5.498 5.622 SPILLED 5.694 5.467 5.560 6.327 NOT TAKEN 5.259 5.846 6.392 5.684 5.843 5.692 6.790 5.837 NOT TAKEN 5.657 6.417 6.478 5.384 6.866 6.280 5.838 SPILLED 5.917 6.000 5.796 SPILLED NOT TAKEN 6.143 5.870 6.068 5.739 5.823 5.698 7.023 6.119 NOT TAKEN 6.029 6.485 6.546 5.429 6.458 5.732 5.618 13.289 5.793 6.181 5.570 6.132 NOT TAKEN SPILLED 5.470 6.398 5.702 6.012 5.706 6.897 6.015 NOT TAKEN 5.807 6.943 6.259 5.081 5.673 5.183 5.725 SPILLED 5.760 5.697 5.459 5.942 NOT TAKEN 6.127 4.969 6.359 5.148 5.757 5.532 6.245 5.808 NOT TAKEN 5.547 6.817 6.681 4.845 5.732 6.220 6.026 SPILLED 6.049 5.781 5.569 6.155 NOT TAKEN 5.820 8.230 5.984 5.531 5.953 5.780 6.173 5.590 NOT TAKEN 5.858 1421 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 39 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 100 PPM C91451 C91452 C91453 C91454 C91455 C91456 C91457 C91458 C91459 C91460 C91461 C91462 C91463 C91464 C91465 C91466 C91467 C91468 C91469 C91470 C91471 C91472 C91473 C91474 C91475 C91476 6.616 6.148 6.228 6.502 6.723 6.012 6.295 6.186 SPILLED 10.941 6.557 6.893 5.165 6.555 7.003 6.074 6.199 6.188 5.974 6.376 6.184 SPILLED 6.621 SPILLED 7.169 5.939 6.324 6.141 6.498 5.960 6.317 5.901 6.651 5.844 SPILLED 6.521 5.602 6.447 5.381 6.184 7.112 5.102 5.948 6.862 5.793 6.604 6.114 SPILLED 10.336 7.961 6.097 5.939 6.293 5.884 6.153 5.784 5.933 5.795 6.698 5.967 SPILLED 5.941 5.223 6.213 4.934 6.335 6.691 5.472 5.514 6.342 5.625 6.543 6.018 6.520 9.406 5.685 5.963 5.948 6.086 5.685 6.970 6.624 5.864 5.933 6.218 5.556 SPILLED 6.379 5.785 6.643 5.442 6.070 6.593 5.342 6.103 6.140 5.989 6.185 6.178 5.850 SPILLED 5.895 5.973 6.018 6.086 5.774 6.569 6.400 6.036 5.884 6.327 6.349 SPILLED 6.211 5.793 6.252 5.768 6.256 6.653 5.333 5.834 5.790 5.551 6.460 6.349 6.951 6.164 6.176 6.388 5.621 6.325 5.989 6.372 5.838 6.337 5.648 6.416 5.938 SPILLED 5.898 6.323 6.129 5.589 5.895 6.880 5.375 5.574 SPILLED 5.391 6.077 6.169 6.231 5.393 5.948 6.150 5.890 6.467 5.911 6.060 6.105 6.399 5.872 6.474 6.355 SPILLED 6.686 6.313 6.286 5.626 7.377 6.081 5.666 6.062 SPILLED 5.744 6.111 6.230 6.126 5.841 6.664 6.336 6.297 6.312 5.874 6.005 5.757 6.082 5.879 5.925 6.004 SPILLED 5.876 6.266 6.180 5.541 6.142 7.113 5.262 6.040 SPILLED 5.764 6.189 6.358 5.753 6.489 5.939 6.122 6.052 6.304 5.721 6.310 6.042 5.811 5.561 5.649 6.034 SPILLED 5.993 5.850 5.716 5.772 6.328 6.901 4.954 6.189 5.805 5.589 5.774 6.128 5.451 5.468 6.911 5.736 6.245 6.299 6.221 5.889 6.180 5.892 5.724 5.864 6.241 SPILLED 6.098 5.483 5.896 5.776 6.253 6.935 5.182 6.542 8.047 6.094 6.214 6.119 5.518 SPILLED 5.694 5.938 5.830 1422 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 40 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 100 PPM C91477 C91478 C91479 C91480 C91481 C91482 C91483 C91484 C91485 C91486 C91487 C91488 C91489 C91490 6.973 7.038 6.884 6.255 5.641 6.576 5.801 6.175 6.073 5.801 6.255 6.172 7.914 6.686 6.343 7.221 6.822 5.825 5.603 6.226 5.725 5.915 5.862 5.832 5.837 5.971 6.699 6.331 6.107 6.252 6.475 5.659 5.727 6.044 5.185 5.845 5.508 5.432 5.336 6.344 6.670 6.300 6.672 6.389 6.699 6.024 5.888 6.627 5.693 5.973 4.879 5.827 5.492 5.900 6.484 6.146 6.438 6.926 6.528 5.825 6.161 6.325 5.725 6.057 5.714 5.664 5.559 6.146 6.526 6.423 6.419 7.357 6.893 5.725 5.655 5.895 6.211 5.682 5.399 5.664 5.416 5.505 6.941 6.154 6.241 6.700 6.892 5.810 5.840 6.072 6.600 5.185 5.918 5.902 6.191 5.763 7.249 6.387 6.540 6.682 6.367 5.745 5.762 5.984 5.685 5.660 5.777 5.913 6.203 5.542 6.305 6.061 6.421 6.561 6.279 6.112 5.622 5.731 5.888 5.885 5.948 6.410 5.892 5.442 6.725 5.944 6.371 SPILLED SPILLED 6.409 5.913 6.362 6.161 5.964 5.940 6.193 6.103 5.381 6.099 6.049 1423 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 41 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 3 PPM C90781 C90782 C90783 C90784 C90785 C90786 C90787 C90788 C90789 C90790 C90791 C90792 C90793 C90794 C90795 C90796 C90797 C90798 C90799 C90800 C90801 C90802 C90803 C90804 C90805 C90806 C90807 C90808 C90809 C90810 C90811 0.116 0.118 0.119 0.130 0.125 0.123 0.126 0.111 NOT TAKEN 0.124 0.137 0.130 0.116 SPILLED 0.116 0.129 NOT TAKEN 0.123 0.124 0.115 0.127 0.134 0.115 0.118 0.124 NOT TAKEN NOT TAKEN 0.118 0.129 0.114 0.122 0.120 0.128 0.133 0.141 0.137 0.131 0.129 0.118 NOT TAKEN 0.131 0.148 0.133 0.122 SPILLED 0.115 0.138 NOT TAKEN 0.129 0.129 0.123 0.136 0.135 0.124 0.120 0.122 NOT TAKEN NOT TAKEN 0.129 0.139 0.124 0.126 0.109 0.114 0.122 0.126 0.119 0.119 0.115 0.103 NOT TAKEN 0.120 0.129 0.121 0.116 0.129 0.107 0.128 NOT TAKEN 0.111 0.119 0.114 0.121 0.121 0.110 0.110 0.113 NOT TAKEN NOT TAKEN 0.116 0.120 0.110 0.111 0.108 0.110 0.113 0.119 0.122 0.113 0.115 0.101 NOT TAKEN 0.112 0.125 0.119 0.112 0.115 0.102 0.123 NOT TAKEN 0.111 0.114 0.109 0.104 0.123 0.106 0.107 0.106 NOT TAKEN NOT TAKEN 0.104 0.123 0.105 0.150 0.102 0.107 0.108 0.116 0.109 0.102 0.108 0.096 NOT TAKEN 0.105 0.120 0.110 0.106 0.101 0.063 0.121 NOT TAKEN 0.104 0.111 0.107 0.134 0.115 0.103 0.100 0.117 NOT TAKEN NOT TAKEN 0.104 0.127 0.097 0.126 0.103 0.107 0.111 0.114 0.108 0.101 0.102 0.091 NOT TAKEN 0.109 0.120 0.008 0.103 0.098 0.016 0.108 NOT TAKEN 0.106 0.106 0.107 0.118 0.109 0.099 0.098 0.033 NOT TAKEN NOT TAKEN 0.100 0.121 0.091 0.107 0.098 0.107 0.108 0.115 0.107 0.107 0.106 0.096 NOT TAKEN 0.102 0.120 NOT TAKEN 0.106 0.100 NOT TAKEN NOT TAKEN NOT TAKEN 0.106 0.108 0.105 0.118 0.110 0.104 0.099 0.015 NOT TAKEN NOT TAKEN 0.107 0.119 0.099 0.088 0.089 0.105 0.106 0.109 0.103 0.114 0.100 0.089 NOT TAKEN 0.106 0.108 NOT TAKEN 0.102 0.091 NOT TAKEN NOT TAKEN NOT TAKEN 0.100 0.106 0.106 0.111 0.103 SPILLED 0.101 NOT TAKEN NOT TAKEN NOT TAKEN 0.020 0.114 0.096 0.100 0.102 0.099 0.102 0.112 0.104 0.104 0.101 0.091 NOT TAKEN 0.096 0.115 NOT TAKEN 0.102 0.092 NOT TAKEN NOT TAKEN NOT TAKEN 0.111 0.106 0.108 0.106 0.112 0.119 0.099 NOT TAKEN NOT TAKEN NOT TAKEN 0.025 0.115 0.097 0.102 0.099 0.110 0.110 0.115 0.108 0.107 0.100 0.090 NOT TAKEN 0.108 0.115 NOT TAKEN 0.100 0.098 NOT TAKEN NOT TAKEN NOT TAKEN 0.107 0.108 0.110 0.108 0.115 0.139 0.098 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.117 0.098 0.103 1424 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 42 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 3 PPM C90812 C90813 C90814 C90815 C90816 C90817 C90818 C90819 C90820 C90821 C90822 C90823 C90824 C90825 C90826 C90827 C90828 C90829 C90830 C90831 C90832 C90833 C90834 C90835 C90836 C90837 C90838 C90839 C90840 0.119 NOT TAKEN NOT TAKEN 0.120 0.116 NOT TAKEN NOT TAKEN 0.111 0.112 0.119 0.131 0.116 0.119 0.114 0.155 0.118 0.114 0.118 0.115 NOT TAKEN 0.116 NOT TAKEN NOT TAKEN 0.117 NOT TAKEN 0.118 0.111 0.112 0.106 0.131 NOT TAKEN NOT TAKEN 0.137 0.120 NOT TAKEN NOT TAKEN 0.122 0.117 0.123 0.134 0.125 0.132 0.124 SPILLED 0.124 0.117 0.118 0.126 NOT TAKEN 0.126 NOT TAKEN NOT TAKEN 0.121 NOT TAKEN 0.124 0.095 0.121 0.118 0.118 NOT TAKEN NOT TAKEN 0.121 0.103 NOT TAKEN NOT TAKEN 0.111 0.107 0.112 0.120 0.113 0.115 0.114 0.130 0.114 0.110 0.107 0.112 NOT TAKEN 0.105 NOT TAKEN NOT TAKEN 0.119 NOT TAKEN 0.111 0.106 0.104 0.103 0.112 NOT TAKEN NOT TAKEN 0.113 0.104 NOT TAKEN NOT TAKEN 0.102 0.102 0.112 0.114 0.112 0.108 0.108 0.129 0.114 0.114 0.099 0.107 NOT TAKEN 0.109 NOT TAKEN NOT TAKEN 0.118 NOT TAKEN 0.110 0.098 0.101 0.093 0.107 NOT TAKEN NOT TAKEN 0.117 0.111 NOT TAKEN NOT TAKEN 0.101 0.095 0.098 0.112 0.099 0.102 0.114 0.127 0.112 0.122 0.096 0.104 NOT TAKEN 0.106 NOT TAKEN NOT TAKEN 0.118 NOT TAKEN 0.131 0.096 0.096 0.097 0.102 NOT TAKEN NOT TAKEN 0.100 0.103 NOT TAKEN NOT TAKEN 0.093 0.095 0.096 0.106 0.102 0.098 0.103 0.119 0.110 0.095 0.090 0.099 NOT TAKEN 0.094 NOT TAKEN NOT TAKEN 0.103 NOT TAKEN 0.183 0.081 0.091 0.090 0.107 NOT TAKEN NOT TAKEN 0.107 0.107 NOT TAKEN NOT TAKEN 0.094 0.095 0.098 0.105 0.097 0.101 0.099 0.117 0.100 0.107 0.091 0.101 NOT TAKEN 0.096 NOT TAKEN NOT TAKEN 0.111 NOT TAKEN 0.231 0.089 0.090 0.091 0.107 NOT TAKEN NOT TAKEN 0.100 0.095 NOT TAKEN NOT TAKEN 0.097 0.099 0.099 0.103 0.098 0.096 0.097 0.068 0.103 0.114 0.089 0.103 NOT TAKEN 0.094 NOT TAKEN NOT TAKEN 0.112 NOT TAKEN 0.201 0.089 0.094 0.092 0.114 NOT TAKEN NOT TAKEN 0.104 0.096 NOT TAKEN NOT TAKEN 0.094 0.044 0.099 0.103 0.094 0.095 0.103 0.111 NOT TAKEN 0.120 0.091 0.101 NOT TAKEN 0.098 NOT TAKEN NOT TAKEN 0.117 NOT TAKEN SPILLED 0.089 0.096 0.092 0.109 NOT TAKEN NOT TAKEN 0.103 0.100 NOT TAKEN NOT TAKEN 0.098 NOT TAKEN 0.095 0.092 0.107 0.099 0.098 0.112 NOT TAKEN 0.116 0.095 0.105 NOT TAKEN 0.033 NOT TAKEN NOT TAKEN 0.107 NOT TAKEN 0.297 0.094 0.088 0.088 1425 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 43 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90841 C90842 C90843 C90844 C90845 C90846 C90847 C90848 C90849 C90850 C90851 C90852 C90853 C90854 C90855 C90856 C90857 C90858 C90859 C90860 C90861 C90862 C90863 C90864 C90865 C90866 C90867 C90868 C90869 C90870 C90871 1.279 1.256 NOT TAKEN 1.256 1.131 NOT TAKEN 1.305 1.107 1.137 1.135 1.205 NOT TAKEN NOT TAKEN 1.338 1.257 1.158 1.178 1.319 1.395 1.557 1.314 1.336 NOT TAKEN 1.306 1.313 NOT TAKEN 1.320 1.140 1.232 1.359 1.167 1.227 1.283 NOT TAKEN 1.405 1.231 NOT TAKEN 1.355 1.217 1.150 1.269 1.293 NOT TAKEN NOT TAKEN 1.558 1.279 1.249 1.297 1.369 1.492 1.527 1.279 1.400 NOT TAKEN 1.341 1.343 NOT TAKEN 1.488 1.207 1.296 1.384 1.283 1.173 1.208 NOT TAKEN 1.212 1.055 NOT TAKEN 1.239 1.149 1.101 1.102 1.151 NOT TAKEN NOT TAKEN 1.258 1.107 1.111 1.155 1.289 1.342 1.373 1.227 1.263 NOT TAKEN 1.214 1.212 NOT TAKEN 1.343 1.089 1.161 1.135 1.167 1.109 1.150 NOT TAKEN 1.138 1.059 NOT TAKEN 1.146 1.023 1.046 1.041 1.135 NOT TAKEN NOT TAKEN 1.166 1.130 1.070 1.244 1.207 1.283 1.380 1.157 1.168 NOT TAKEN 1.179 1.231 NOT TAKEN 1.259 1.006 1.098 1.126 1.097 1.153 1.136 NOT TAKEN 1.156 1.058 NOT TAKEN 1.110 1.026 1.032 1.096 1.120 NOT TAKEN NOT TAKEN 1.115 1.075 0.988 1.129 1.150 1.232 1.323 1.161 1.133 NOT TAKEN 1.141 1.192 NOT TAKEN 1.289 1.039 1.102 1.092 1.128 1.018 1.041 NOT TAKEN 0.996 0.997 NOT TAKEN 1.021 0.922 0.977 1.049 1.041 NOT TAKEN NOT TAKEN 1.089 0.988 0.927 1.087 1.104 1.034 1.306 1.086 1.108 NOT TAKEN 1.100 1.100 NOT TAKEN 1.303 1.004 1.048 1.082 1.059 1.052 1.084 NOT TAKEN 1.153 0.989 NOT TAKEN 1.078 0.937 0.916 1.048 1.100 NOT TAKEN NOT TAKEN 1.138 1.011 1.061 1.054 1.071 0.984 1.296 1.142 1.092 NOT TAKEN 1.075 1.155 NOT TAKEN 1.310 0.965 1.046 1.063 1.116 1.049 0.980 NOT TAKEN 1.059 1.005 NOT TAKEN 1.046 0.823 0.813 0.961 0.994 NOT TAKEN NOT TAKEN 1.078 0.920 0.987 1.089 1.024 0.597 1.181 0.794 0.567 NOT TAKEN 1.124 1.077 NOT TAKEN 1.293 0.967 1.022 1.052 1.018 1.039 1.039 NOT TAKEN 1.072 0.985 NOT TAKEN 1.097 0.866 0.729 0.977 1.010 NOT TAKEN NOT TAKEN 1.156 0.955 0.948 1.099 1.050 1.455 1.196 0.547 0.881 NOT TAKEN 1.094 1.133 NOT TAKEN 1.250 0.974 0.813 1.011 1.043 1.116 1.086 NOT TAKEN 1.120 1.019 NOT TAKEN 1.121 0.773 0.678 1.071 1.074 NOT TAKEN NOT TAKEN 1.105 1.001 0.985 1.131 1.043 NOT TAKEN 1.287 NOT TAKEN NOT TAKEN NOT TAKEN 1.052 1.091 NOT TAKEN 1.408 0.367 1.098 0.878 1.035 1426 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 44 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90872 C90873 C90874 C90875 C90876 C90877 C90878 C90879 C90880 C90881 C90882 C90883 C90884 C90885 C90886 C90887 C90888 C90889 C90890 C90891 C90892 C90893 C90894 C90895 C90896 C90897 C90898 C90899 C90900 1.183 1.246 1.107 1.171 SPILLED NOT TAKEN 1.368 1.282 NOT TAKEN 1.164 1.212 1.461 1.517 1.206 1.240 1.137 1.341 1.179 NOT TAKEN NOT TAKEN 1.290 1.199 1.242 1.260 1.399 1.187 1.227 1.357 1.300 1.311 1.288 1.169 1.251 1.265 NOT TAKEN 1.448 1.253 NOT TAKEN 1.248 1.257 1.660 1.549 1.273 1.316 1.287 1.525 1.273 NOT TAKEN NOT TAKEN 1.381 1.391 1.289 1.384 1.477 1.254 1.334 1.464 1.411 1.157 1.157 1.066 1.174 1.108 NOT TAKEN 1.268 1.184 NOT TAKEN 1.132 1.134 1.522 1.429 1.162 1.178 1.145 1.300 1.168 NOT TAKEN NOT TAKEN 1.237 1.209 1.269 1.191 1.332 1.131 1.113 1.282 1.255 1.085 1.079 1.011 1.136 1.025 NOT TAKEN 1.252 1.187 NOT TAKEN 1.078 1.123 1.511 1.250 1.088 1.169 1.085 1.132 1.116 NOT TAKEN NOT TAKEN 1.154 1.220 1.202 1.194 1.281 1.070 1.167 1.260 1.215 1.051 SPILLED 1.000 1.071 1.040 NOT TAKEN 1.271 1.051 NOT TAKEN 1.032 1.095 1.021 1.339 1.053 1.049 1.061 1.168 1.134 NOT TAKEN NOT TAKEN 1.140 1.181 1.136 1.137 1.285 1.068 1.139 1.272 1.203 1.017 1.122 0.178 1.040 NOT TAKEN NOT TAKEN NOT TAKEN 1.059 NOT TAKEN 0.975 1.013 1.480 1.363 0.975 1.122 1.019 1.129 1.003 NOT TAKEN NOT TAKEN 1.058 1.086 1.039 1.078 1.301 1.023 1.220 1.238 1.158 1.048 1.069 1.231 1.114 NOT TAKEN NOT TAKEN NOT TAKEN 1.106 NOT TAKEN 1.018 0.999 1.418 1.397 0.994 1.066 0.924 1.205 1.109 NOT TAKEN NOT TAKEN 1.082 1.030 1.046 1.049 1.088 0.999 0.223 1.173 1.104 1.033 SPILLED 1.363 1.039 NOT TAKEN NOT TAKEN NOT TAKEN 1.104 NOT TAKEN 0.974 0.962 1.169 SPILLED 1.039 0.908 0.896 1.369 1.018 NOT TAKEN NOT TAKEN 1.145 0.975 0.977 1.011 0.813 0.956 NOT TAKEN 1.077 1.034 1.024 0.986 NOT TAKEN 1.044 NOT TAKEN NOT TAKEN NOT TAKEN 1.190 NOT TAKEN 1.115 0.953 1.257 SPILLED 0.985 1.050 0.000 1.256 1.038 NOT TAKEN NOT TAKEN 1.018 1.032 0.940 0.980 0.465 0.986 NOT TAKEN 1.073 1.030 1.061 0.916 NOT TAKEN 1.095 NOT TAKEN NOT TAKEN NOT TAKEN 1.118 NOT TAKEN 1.069 1.016 1.198 SPILLED 0.946 1.076 NOT TAKEN 1.402 1.041 NOT TAKEN NOT TAKEN 0.648 0.999 1.057 1.079 NOT TAKEN 0.992 NOT TAKEN 1.070 1.066 1427 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 45 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90901 C90902 C90903 C90904 C90905 C90906 C90907 C90908 C90909 C90910 C90911 C90912 C90913 C90914 C90915 C90916 C90917 C90918 C90919 C90920 C90921 C90922 C90923 C90924 C90925 C90926 C90927 C90928 C90929 C90930 C90931 4.325 4.144 4.137 4.457 NOT TAKEN 4.475 NOT TAKEN 3.799 4.105 4.119 4.276 4.291 3.980 3.895 4.242 4.384 NOT TAKEN 4.434 4.333 3.979 NOT TAKEN 4.408 4.765 4.081 4.629 4.289 4.520 4.204 4.617 4.649 4.188 4.592 4.363 4.403 4.738 NOT TAKEN 4.654 NOT TAKEN 4.167 4.116 4.289 4.477 4.538 4.359 3.982 4.214 4.586 NOT TAKEN 4.399 4.368 4.282 NOT TAKEN 4.471 4.460 4.361 4.805 4.410 4.714 4.512 4.596 4.834 4.526 4.202 3.921 4.101 4.361 NOT TAKEN 4.206 NOT TAKEN 3.579 4.005 3.857 4.204 4.118 3.860 3.722 4.026 4.147 NOT TAKEN 4.094 4.277 3.992 NOT TAKEN 4.103 4.553 4.383 4.615 4.191 3.912 3.925 3.755 4.404 4.148 4.065 3.872 3.951 4.168 NOT TAKEN 3.893 NOT TAKEN 3.676 3.655 3.558 3.943 4.038 3.887 3.534 3.889 3.979 NOT TAKEN 3.941 4.095 3.995 NOT TAKEN 3.894 4.201 4.243 4.183 3.999 3.989 3.861 3.768 4.117 3.934 4.003 3.773 3.557 4.124 NOT TAKEN 3.808 NOT TAKEN 3.758 3.447 3.479 3.933 3.923 3.558 3.653 3.908 3.825 NOT TAKEN 3.615 4.093 4.005 NOT TAKEN 3.961 4.221 4.156 4.170 4.104 3.945 3.757 3.615 4.155 3.527 3.903 NOT TAKEN 3.513 3.915 NOT TAKEN 3.711 NOT TAKEN 3.859 NOT TAKEN 3.304 3.714 3.865 NOT TAKEN 3.574 3.731 3.607 NOT TAKEN NOT TAKEN 3.794 3.952 NOT TAKEN 3.697 4.247 NOT TAKEN 4.061 3.468 3.697 3.962 4.249 4.039 3.570 3.659 NOT TAKEN 3.523 3.937 NOT TAKEN 3.481 NOT TAKEN 3.632 NOT TAKEN 3.269 3.528 3.786 NOT TAKEN 3.369 3.589 3.439 NOT TAKEN NOT TAKEN 3.787 3.923 NOT TAKEN 2.906 4.063 NOT TAKEN 3.856 3.384 3.622 3.720 3.425 3.881 3.536 3.771 NOT TAKEN 3.375 3.913 NOT TAKEN 3.282 NOT TAKEN 3.839 NOT TAKEN 3.405 3.468 2.336 NOT TAKEN 3.466 3.585 3.491 NOT TAKEN NOT TAKEN 3.833 3.603 NOT TAKEN NOT TAKEN 3.940 NOT TAKEN 3.649 3.194 3.369 3.548 3.804 3.637 3.410 3.755 NOT TAKEN 3.600 3.850 NOT TAKEN 3.574 NOT TAKEN 3.806 NOT TAKEN 3.389 3.582 3.207 NOT TAKEN 3.634 3.698 3.565 NOT TAKEN NOT TAKEN 4.133 3.913 NOT TAKEN NOT TAKEN 3.989 NOT TAKEN 3.875 3.314 3.624 3.479 NOT TAKEN 4.005 3.190 2.387 NOT TAKEN 3.464 3.764 NOT TAKEN 3.721 NOT TAKEN 3.774 NOT TAKEN 3.431 3.535 3.136 NOT TAKEN 3.497 3.669 3.609 NOT TAKEN NOT TAKEN 4.192 4.236 NOT TAKEN NOT TAKEN 4.172 NOT TAKEN 3.874 3.515 3.714 3.724 NOT TAKEN 4.367 3.525 1428 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 46 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90932 C90933 C90934 C90935 C90936 C90937 C90938 C90939 C90940 C90941 C90942 C90943 C90944 C90945 C90946 C90947 C90948 C90949 C90950 C90951 C90952 C90953 C90954 C90955 C90956 C90957 C90958 C90959 C90960 C90961 C90962 5.156 4.279 4.858 NOT TAKEN 4.376 5.060 4.751 NOT TAKEN 4.245 4.285 NOT TAKEN 5.510 4.267 4.435 3.180 4.179 4.337 NOT TAKEN 4.221 4.214 4.014 3.957 4.582 4.376 4.130 4.526 4.459 4.375 NOT TAKEN NOT TAKEN 4.289 5.310 4.330 4.840 NOT TAKEN 3.991 5.997 4.767 NOT TAKEN 4.735 4.583 NOT TAKEN 5.048 4.528 4.443 4.498 4.680 4.670 NOT TAKEN 4.592 4.436 4.305 4.184 4.797 4.561 4.626 4.289 3.483 4.580 NOT TAKEN NOT TAKEN 4.474 4.780 3.753 4.620 NOT TAKEN 4.584 4.847 4.483 NOT TAKEN 4.176 3.794 NOT TAKEN SPILLED 4.254 NOT TAKEN 2.861 4.034 4.071 NOT TAKEN 3.969 3.837 3.625 3.909 4.353 4.141 3.948 4.293 4.049 4.143 NOT TAKEN NOT TAKEN 4.020 4.406 3.867 4.350 NOT TAKEN 4.285 4.715 4.160 NOT TAKEN 4.346 3.936 NOT TAKEN 4.391 4.105 NOT TAKEN 3.554 3.920 3.940 NOT TAKEN 4.011 3.865 3.352 3.725 4.129 3.990 4.001 4.519 4.082 3.952 NOT TAKEN NOT TAKEN 3.887 4.236 3.088 4.817 NOT TAKEN 3.531 4.259 4.147 NOT TAKEN 3.893 3.804 NOT TAKEN 4.209 3.758 NOT TAKEN 3.928 3.900 4.007 NOT TAKEN 3.584 3.842 2.767 3.736 3.915 3.850 3.854 4.182 3.614 3.764 NOT TAKEN NOT TAKEN 3.590 NOT TAKEN 3.902 4.504 NOT TAKEN 3.948 4.233 3.802 NOT TAKEN 3.871 3.580 NOT TAKEN 4.608 3.817 NOT TAKEN 4.117 3.579 3.798 NOT TAKEN NOT TAKEN NOT TAKEN 1.492 3.592 3.681 3.683 3.911 NOT TAKEN 3.929 3.587 NOT TAKEN NOT TAKEN 3.612 NOT TAKEN 3.430 4.519 NOT TAKEN 3.928 4.185 4.008 NOT TAKEN 2.244 3.549 NOT TAKEN 4.657 3.927 NOT TAKEN 3.358 3.584 3.673 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.528 3.741 3.654 3.803 NOT TAKEN 2.009 3.421 NOT TAKEN NOT TAKEN 3.455 NOT TAKEN 3.613 4.155 NOT TAKEN 2.485 3.939 3.860 NOT TAKEN NOT TAKEN 3.508 NOT TAKEN SPILLED 3.758 NOT TAKEN 3.763 3.587 3.788 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.517 2.884 3.752 3.747 NOT TAKEN 4.171 3.549 NOT TAKEN NOT TAKEN 3.255 NOT TAKEN 3.069 3.084 NOT TAKEN 3.259 3.887 0.913 NOT TAKEN NOT TAKEN 3.230 NOT TAKEN 4.002 3.611 NOT TAKEN 4.128 3.350 3.703 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.569 2.134 3.777 3.282 NOT TAKEN 4.289 3.434 NOT TAKEN NOT TAKEN 1.665 NOT TAKEN 3.587 4.162 NOT TAKEN 4.031 4.328 NOT TAKEN NOT TAKEN NOT TAKEN 3.481 NOT TAKEN 3.867 3.821 NOT TAKEN 4.204 3.574 3.757 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.468 NOT TAKEN 3.641 3.653 NOT TAKEN 4.078 3.524 NOT TAKEN NOT TAKEN NOT TAKEN 1429 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 47 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90963 C90964 C90965 C90966 C90967 C90968 C90969 C90970 4.010 4.047 6.322 NOT TAKEN 4.162 4.216 4.305 4.067 4.265 4.162 6.257 NOT TAKEN 4.341 4.533 4.601 4.280 3.747 3.782 5.008 NOT TAKEN 3.903 3.833 4.310 4.055 3.691 3.648 5.442 NOT TAKEN 3.676 3.688 4.098 3.610 3.648 3.668 4.784 NOT TAKEN 3.497 3.635 3.820 3.747 3.449 4.297 4.993 NOT TAKEN NOT TAKEN 3.321 3.615 3.357 3.213 3.788 4.932 NOT TAKEN NOT TAKEN 3.479 3.593 3.591 1.518 2.508 3.624 NOT TAKEN NOT TAKEN 3.537 3.301 3.265 2.256 2.658 SPILLED NOT TAKEN NOT TAKEN 0.052 3.544 3.661 2.672 SPILLED SPILLED NOT TAKEN NOT TAKEN 4.625 3.308 3.402 1430 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 48 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 100 PPM C91041 C91042 C91043 C91044 C91045 C91046 C91047 C91048 C91049 4.154 4.291 4.545 4.599 4.194 NOT TAKEN 4.673 4.361 4.116 4.322 4.217 4.691 5.054 4.347 NOT TAKEN 5.322 4.518 1.039 4.091 3.896 4.184 4.482 3.838 NOT TAKEN 4.210 4.195 NOT TAKEN 3.875 4.016 3.851 4.454 3.166 NOT TAKEN 4.723 4.183 NOT TAKEN 3.701 3.624 3.602 4.272 NOT TAKEN NOT TAKEN 4.571 4.043 NOT TAKEN 1431 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 49 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 100 PPM C91050 C91051 C91052 C91053 C91054 C91055 C91056 C91057 C91058 C91059 C91060 C91061 C91062 C91063 C91064 C91065 C91066 C91067 C91068 C91069 C91070 C91071 C91072 C91073 C91074 C91075 C91076 C91077 C91078 C91079 C91080 4.234 4.568 4.087 4.160 4.110 4.336 4.369 4.162 4.208 3.860 4.408 4.007 5.042 4.654 4.261 4.370 4.120 4.347 NOT TAKEN 4.439 4.362 3.985 4.184 3.895 4.426 4.330 4.123 4.852 4.161 4.014 4.576 4.707 4.602 4.131 4.278 4.180 4.008 SPILLED 4.475 4.500 4.209 4.579 4.260 4.530 4.531 4.547 4.519 4.192 4.290 NOT TAKEN 4.516 4.799 4.126 4.535 4.096 4.631 4.439 4.376 4.920 3.814 4.486 4.461 4.127 4.166 3.874 3.927 3.832 3.948 4.143 4.159 4.182 3.685 4.063 3.771 4.054 4.214 4.254 3.990 3.974 3.836 NOT TAKEN 3.817 4.630 3.748 4.230 3.911 4.575 4.164 3.956 4.459 3.792 4.037 4.148 3.991 4.318 3.760 4.044 3.652 3.658 3.974 4.378 3.862 3.994 4.056 3.872 4.130 4.143 3.919 3.807 3.934 3.741 NOT TAKEN 3.798 4.215 3.742 3.882 3.825 4.176 3.976 4.164 3.995 4.225 4.178 4.165 3.904 4.011 3.757 3.922 3.611 3.420 3.873 3.904 3.831 4.001 3.811 3.736 3.822 4.088 3.773 3.908 3.764 3.646 NOT TAKEN 3.516 3.916 3.509 3.767 3.737 3.885 3.620 3.965 3.761 3.967 3.894 4.130 1432 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 50 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91191 C91192 C91193 C91194 C91195 C91196 C91197 C91198 C91199 C91200 C91201 C91202 C91203 C91204 C91205 C91206 C91207 C91208 C91209 0.135 NOT TAKEN 0.221 0.159 0.179 0.139 0.194 NOT TAKEN 0.171 0.174 0.178 0.163 0.145 0.172 NOT TAKEN 0.164 0.171 0.192 0.215 0.153 NOT TAKEN 0.191 0.175 0.181 0.176 0.210 NOT TAKEN 0.184 0.218 0.183 0.172 0.152 0.176 NOT TAKEN 0.163 0.173 0.191 0.225 0.159 NOT TAKEN 0.183 0.150 0.146 0.163 0.194 NOT TAKEN 0.163 0.158 0.150 0.135 0.141 0.158 NOT TAKEN 0.149 0.163 0.161 0.171 0.098 NOT TAKEN 0.163 0.149 0.160 0.142 0.156 NOT TAKEN 0.157 0.155 0.146 0.139 0.134 0.179 NOT TAKEN 0.155 0.154 0.157 0.156 SPILLED NOT TAKEN 0.129 0.132 0.163 0.130 0.135 NOT TAKEN 0.152 0.148 0.146 0.144 0.126 0.144 NOT TAKEN 0.147 0.155 0.164 0.166 0.140 NOT TAKEN 0.142 0.095 0.137 0.135 0.117 NOT TAKEN 0.146 0.132 0.149 0.125 0.109 0.151 NOT TAKEN 0.137 0.112 0.144 0.146 0.123 NOT TAKEN 0.147 0.140 0.135 0.138 0.130 NOT TAKEN 0.146 0.138 0.120 0.128 0.133 0.095 NOT TAKEN 0.147 0.162 0.138 0.139 0.140 NOT TAKEN 0.120 0.131 0.146 0.121 0.124 NOT TAKEN 0.138 0.147 0.155 0.131 0.131 NOT TAKEN NOT TAKEN 0.104 0.134 0.132 0.151 0.136 NOT TAKEN 0.128 0.156 0.139 0.130 0.120 NOT TAKEN 0.136 0.150 0.105 0.117 0.119 NOT TAKEN NOT TAKEN 0.123 0.125 0.113 0.166 0.123 NOT TAKEN 0.130 0.152 0.116 0.119 0.118 NOT TAKEN 0.139 0.122 0.137 0.119 0.122 NOT TAKEN NOT TAKEN 0.181 0.138 0.105 0.195 1433 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 51 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91210 C91211 C91212 C91213 C91214 C91215 C91216 C91217 C91218 C91219 C91220 C91221 C91222 C91223 C91224 C91225 C91226 C91227 C91228 C91229 C91230 C91231 C91232 C91233 C91234 C91235 C91236 C91237 C91238 C91239 C91240 0.179 0.141 0.175 0.141 0.178 0.191 0.186 0.163 0.223 SPILLED 0.151 0.176 NOT TAKEN NOT TAKEN 0.203 0.177 0.144 0.151 0.176 0.167 NOT TAKEN 0.135 0.178 NOT TAKEN 0.119 SPILLED 0.233 NOT TAKEN 0.189 0.164 0.175 0.175 0.155 0.172 0.169 0.178 0.195 0.182 0.186 0.240 0.261 0.167 0.182 NOT TAKEN NOT TAKEN 0.227 0.179 0.165 0.157 0.184 0.181 NOT TAKEN 0.154 0.192 NOT TAKEN 0.138 0.255 0.234 NOT TAKEN 0.181 0.148 0.164 0.159 0.139 0.159 0.154 0.149 0.147 0.168 0.180 0.236 0.187 0.143 0.178 NOT TAKEN NOT TAKEN 0.181 0.152 0.150 0.142 0.176 0.165 NOT TAKEN 0.160 0.164 NOT TAKEN 0.138 0.207 0.211 NOT TAKEN 0.164 0.159 0.134 0.138 0.117 0.142 0.146 0.144 0.142 0.152 0.146 0.194 0.174 0.128 0.157 NOT TAKEN NOT TAKEN 0.142 0.162 0.146 0.137 0.161 0.161 NOT TAKEN 0.148 0.170 NOT TAKEN 0.127 0.189 0.197 NOT TAKEN 0.158 0.145 0.165 0.135 0.119 0.142 0.145 0.141 0.204 0.143 0.161 0.163 0.150 0.123 0.135 NOT TAKEN NOT TAKEN 0.165 0.158 0.146 0.133 0.163 0.159 NOT TAKEN 0.126 0.149 NOT TAKEN 0.113 0.156 0.189 NOT TAKEN 0.145 0.149 SPILLED 0.126 0.128 0.148 0.117 0.127 NOT TAKEN 0.147 0.140 0.204 0.147 0.122 0.129 NOT TAKEN NOT TAKEN 0.158 0.147 0.138 0.128 SPILLED 0.144 NOT TAKEN 0.134 0.158 NOT TAKEN 0.107 0.144 0.178 NOT TAKEN 0.146 0.132 0.116 0.141 0.112 0.143 0.127 0.136 NOT TAKEN 0.160 0.146 SPILLED 0.143 0.122 0.136 NOT TAKEN NOT TAKEN 0.143 0.151 0.141 0.116 0.175 0.137 NOT TAKEN 0.121 0.154 NOT TAKEN 0.108 0.160 0.170 NOT TAKEN 0.138 NOT TAKEN 0.139 0.126 0.123 0.002 0.115 0.139 NOT TAKEN 0.173 0.133 0.196 0.135 0.110 0.140 NOT TAKEN NOT TAKEN 0.132 0.135 0.137 0.124 0.163 0.144 NOT TAKEN 0.116 0.160 NOT TAKEN 0.063 0.148 0.164 NOT TAKEN 0.021 NOT TAKEN 0.141 0.122 0.129 NOT TAKEN 0.109 0.148 NOT TAKEN 0.178 NOT TAKEN SPILLED 0.155 0.121 0.134 NOT TAKEN NOT TAKEN 0.140 0.129 0.134 0.120 0.160 0.142 NOT TAKEN 0.115 0.159 NOT TAKEN NOT TAKEN 0.165 0.166 NOT TAKEN NOT TAKEN NOT TAKEN 0.156 0.124 0.131 NOT TAKEN 0.066 0.149 NOT TAKEN 0.173 NOT TAKEN SPILLED 0.138 0.129 0.132 NOT TAKEN NOT TAKEN 0.142 0.139 0.126 0.129 0.171 0.163 NOT TAKEN 0.114 0.158 NOT TAKEN NOT TAKEN 0.171 0.174 NOT TAKEN NOT TAKEN NOT TAKEN 0.150 1434 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 52 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91241 C91242 C91243 C91244 C91245 C91246 C91247 C91248 C91249 C91250 NOT TAKEN 0.264 0.275 0.136 0.160 SPILLED 0.176 NOT TAKEN 0.160 0.184 NOT TAKEN SPILLED 0.213 0.193 0.155 SPILLED 0.192 NOT TAKEN 0.162 0.187 NOT TAKEN SPILLED 0.154 0.169 0.154 SPILLED 0.172 NOT TAKEN 0.145 0.161 NOT TAKEN 0.183 0.170 0.164 0.150 SPILLED 0.152 NOT TAKEN 0.136 0.158 NOT TAKEN SPILLED 0.133 0.172 0.128 SPILLED 0.148 NOT TAKEN 0.127 0.167 NOT TAKEN 0.176 0.132 0.166 0.123 SPILLED 0.122 NOT TAKEN 0.125 0.169 NOT TAKEN 0.182 0.134 0.164 0.128 SPILLED 0.131 NOT TAKEN 0.124 0.166 NOT TAKEN SPILLED 0.150 0.118 0.122 0.161 0.079 NOT TAKEN 0.117 0.139 NOT TAKEN SPILLED 0.145 NOT TAKEN 0.119 0.169 0.122 NOT TAKEN 0.125 0.142 NOT TAKEN 0.153 0.134 NOT TAKEN 0.132 SPILLED 0.075 NOT TAKEN 0.110 0.132 GROUP: FEMALE 3 - 30 PPM C91251 C91252 C91253 C91254 C91255 C91256 C91257 C91258 C91259 C91260 C91261 C91262 C91263 C91264 C91265 C91266 C91267 C91268 NOT TAKEN 1.441 1.728 1.684 1.639 1.817 1.753 1.670 1.636 1.511 1.816 1.858 1.622 1.670 1.736 1.961 1.741 1.533 NOT TAKEN 1.602 1.702 1.777 1.746 1.865 1.747 1.776 1.757 1.592 1.926 1.947 1.647 1.845 1.572 1.959 1.868 1.545 NOT TAKEN 1.529 1.465 1.685 1.653 1.712 1.554 1.641 1.611 1.490 1.839 1.643 1.563 1.664 1.434 1.699 1.618 1.433 NOT TAKEN 1.394 1.474 1.538 1.544 1.542 1.540 1.424 1.285 1.385 1.752 1.694 1.466 1.634 1.649 1.631 1.638 1.361 NOT TAKEN 1.307 1.341 1.508 1.478 1.480 1.479 1.295 1.254 1.296 1.612 1.654 1.387 1.501 1.521 1.506 1.564 1.349 NOT TAKEN 1.183 1.103 1.355 1.534 1.554 1.447 1.353 0.788 1.269 1.590 1.567 1.333 1.425 1.281 1.481 1.643 1.160 NOT TAKEN 1.035 1.224 1.353 1.421 1.484 1.353 1.394 0.941 1.257 1.516 1.518 1.298 1.371 1.495 1.485 1.863 1.261 NOT TAKEN 1.135 1.317 1.444 1.295 1.426 1.344 1.307 NOT TAKEN 1.078 1.446 1.456 0.832 1.337 1.344 1.392 1.670 1.170 NOT TAKEN 1.186 1.291 1.442 1.346 1.510 1.179 1.371 NOT TAKEN 0.543 1.337 1.611 1.716 1.337 1.251 1.500 NOT TAKEN 1.255 NOT TAKEN 1.153 1.238 1.356 1.375 1.629 1.240 1.320 NOT TAKEN 1.068 1.388 1.553 NOT TAKEN 1.304 1.329 1.488 NOT TAKEN 1.435 1435 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 30 PPM C91269 C91270 C91271 C91272 C91273 C91274 C91275 C91276 C91277 C91278 C91279 C91280 C91281 C91282 C91283 C91284 C91285 C91286 C91287 C91288 C91289 C91290 C91291 C91292 C91293 C91294 C91295 C91296 C91297 C91298 C91299 1.548 1.861 1.834 NOT TAKEN 1.803 1.514 1.566 2.030 1.821 1.779 1.783 1.785 NOT TAKEN 1.578 1.678 1.829 NOT TAKEN 2.448 NOT TAKEN NOT TAKEN 1.785 2.119 NOT TAKEN 1.447 NOT TAKEN 1.580 1.528 1.846 2.111 1.930 NOT TAKEN 1.876 2.021 2.271 NOT TAKEN 1.826 1.604 1.667 2.059 1.795 1.799 1.777 1.846 NOT TAKEN 1.824 1.658 1.699 NOT TAKEN 2.337 NOT TAKEN NOT TAKEN 2.125 2.137 NOT TAKEN 1.676 NOT TAKEN 1.797 1.735 2.144 SPILLED 2.881 NOT TAKEN 1.622 1.745 1.954 NOT TAKEN 1.846 1.480 1.663 1.820 1.652 1.591 1.620 1.621 NOT TAKEN 1.476 1.606 1.731 NOT TAKEN 2.000 NOT TAKEN NOT TAKEN 1.606 1.971 NOT TAKEN 1.457 NOT TAKEN 1.632 1.646 1.933 1.681 2.346 NOT TAKEN 1.563 1.135 1.710 NOT TAKEN 1.700 1.392 1.360 1.834 1.561 1.441 1.497 1.505 NOT TAKEN 0.857 1.404 1.258 NOT TAKEN 1.808 NOT TAKEN NOT TAKEN 1.468 1.851 NOT TAKEN 1.388 NOT TAKEN 1.610 1.652 1.788 1.677 2.127 NOT TAKEN 1.514 1.769 1.657 NOT TAKEN 1.555 1.389 1.284 1.702 SPILLED 1.413 1.408 1.540 NOT TAKEN 0.144 1.418 1.362 NOT TAKEN 1.842 NOT TAKEN NOT TAKEN 1.326 1.857 NOT TAKEN 1.307 NOT TAKEN 1.200 1.512 1.722 1.538 SPILLED NOT TAKEN 1.577 1.407 1.608 NOT TAKEN 1.535 1.461 1.199 1.663 1.507 1.359 1.475 1.510 NOT TAKEN NOT TAKEN 1.374 1.380 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 1.108 0.902 NOT TAKEN 1.347 NOT TAKEN 1.480 1.352 1.641 1.623 SPILLED NOT TAKEN 1.366 NOT TAKEN 1.660 NOT TAKEN 1.426 1.298 1.066 1.685 1.542 1.355 SPILLED 1.456 NOT TAKEN NOT TAKEN 1.306 1.213 NOT TAKEN 1.677 NOT TAKEN NOT TAKEN 1.313 NOT TAKEN NOT TAKEN 1.305 NOT TAKEN 1.336 1.391 1.562 1.623 SPILLED NOT TAKEN 1.321 NOT TAKEN 1.472 NOT TAKEN 1.501 1.317 SPILLED 1.557 1.479 1.425 1.371 1.359 NOT TAKEN NOT TAKEN 1.114 1.240 NOT TAKEN 1.603 NOT TAKEN NOT TAKEN 1.964 NOT TAKEN NOT TAKEN 1.133 NOT TAKEN 1.278 1.364 1.612 1.410 1.641 NOT TAKEN 1.369 NOT TAKEN 1.630 NOT TAKEN 1.594 1.205 1.986 1.645 1.461 1.398 1.759 1.408 NOT TAKEN NOT TAKEN 1.224 1.457 NOT TAKEN 1.788 NOT TAKEN NOT TAKEN 1.350 NOT TAKEN NOT TAKEN 1.203 NOT TAKEN 1.446 1.264 1.629 1.419 1.595 NOT TAKEN 1.293 NOT TAKEN 1.297 NOT TAKEN 1.558 NOT TAKEN NOT TAKEN 1.642 NOT TAKEN 1.373 1.507 1.352 NOT TAKEN NOT TAKEN 1.282 1.296 NOT TAKEN 1.532 NOT TAKEN NOT TAKEN 2.053 NOT TAKEN NOT TAKEN 1.165 NOT TAKEN 1.608 1.366 1.723 1.456 1.769 NOT TAKEN 1436 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 54 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 30 PPM C91300 C91301 C91302 C91303 C91304 C91305 C91306 C91307 C91308 C91309 C91310 1.681 1.295 1.796 1.338 NOT TAKEN 1.820 1.955 1.625 SPILLED 1.646 1.693 1.867 1.632 1.843 1.409 NOT TAKEN 1.782 2.076 1.702 2.500 1.727 1.847 1.594 1.457 1.742 1.402 NOT TAKEN 1.677 2.018 1.486 2.205 1.591 1.719 1.500 1.390 1.716 1.296 NOT TAKEN 1.492 1.799 1.575 2.002 1.516 1.679 1.373 1.385 1.487 1.248 NOT TAKEN 1.511 1.666 1.411 1.785 1.409 1.532 1.393 1.289 0.777 1.221 NOT TAKEN 1.353 1.791 1.473 1.645 1.371 1.449 1.124 1.194 1.866 1.074 NOT TAKEN 1.245 1.746 1.474 1.450 1.296 1.417 1.055 1.203 1.949 1.252 NOT TAKEN 1.311 1.541 1.360 1.389 1.166 1.315 1.193 1.311 1.386 1.203 NOT TAKEN 1.302 1.591 1.453 1.449 NOT TAKEN 1.392 1.319 1.231 NOT TAKEN 1.068 NOT TAKEN 1.232 1.559 1.366 NOT TAKEN NOT TAKEN 1.351 GROUP: FEMALE 4 - 100 PPM C91311 C91312 C91313 C91314 C91315 C91316 C91317 C91318 C91319 C91320 C91321 C91322 C91323 C91324 C91325 C91326 C91327 7.211 6.001 7.172 9.844 6.453 NOT TAKEN NOT TAKEN NOT TAKEN 6.521 6.415 6.599 NOT TAKEN 7.249 5.826 5.832 7.175 6.025 7.702 6.303 6.964 10.963 6.951 NOT TAKEN NOT TAKEN NOT TAKEN 6.549 6.408 7.370 NOT TAKEN 7.542 6.367 6.212 7.578 6.477 6.279 5.618 6.342 7.828 6.298 NOT TAKEN NOT TAKEN NOT TAKEN 6.008 5.739 6.557 NOT TAKEN 6.994 5.740 5.384 6.624 5.511 6.410 5.742 6.063 10.274 6.150 NOT TAKEN NOT TAKEN NOT TAKEN 5.570 5.748 6.092 NOT TAKEN 6.459 5.752 5.066 6.864 5.921 5.969 5.387 5.660 7.273 5.927 NOT TAKEN NOT TAKEN NOT TAKEN 5.383 5.552 5.950 NOT TAKEN 5.753 5.599 4.426 6.597 5.750 5.764 5.298 5.519 8.336 5.554 NOT TAKEN NOT TAKEN NOT TAKEN 5.418 5.362 5.479 NOT TAKEN NOT TAKEN 5.104 5.060 6.404 5.647 5.863 5.369 5.323 SPILLED 5.356 NOT TAKEN NOT TAKEN NOT TAKEN 5.229 5.037 5.400 NOT TAKEN NOT TAKEN 5.342 5.296 5.970 5.495 NOT TAKEN 5.274 4.902 SPILLED 5.289 NOT TAKEN NOT TAKEN NOT TAKEN 4.839 4.827 4.861 NOT TAKEN NOT TAKEN 5.640 5.146 5.568 5.260 NOT TAKEN 5.541 5.095 8.267 5.062 NOT TAKEN NOT TAKEN NOT TAKEN 4.951 4.959 5.552 NOT TAKEN NOT TAKEN 5.650 5.628 6.180 5.414 NOT TAKEN 5.519 5.069 4.753 4.866 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.666 4.807 NOT TAKEN NOT TAKEN 5.628 5.088 5.517 5.857 1437 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 55 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91328 C91329 C91330 C91331 C91332 C91333 C91334 C91335 C91336 C91337 C91338 C91339 C91340 C91341 C91342 C91343 C91344 C91345 C91346 C91347 C91348 C91349 C91350 C91351 C91352 C91353 C91354 C91355 C91356 C91357 C91358 5.154 NOT TAKEN 6.295 5.410 6.400 NOT TAKEN 6.108 5.717 15.370 NOT TAKEN 5.288 6.436 5.244 6.502 6.086 6.233 5.680 NOT TAKEN 5.698 SPILLED 6.033 SPILLED NOT TAKEN 5.311 6.030 6.946 5.933 NOT TAKEN SPILLED 5.551 5.872 6.601 NOT TAKEN 6.903 5.850 7.012 NOT TAKEN 6.132 6.483 12.687 NOT TAKEN 6.185 6.571 6.134 6.975 6.316 6.475 5.997 NOT TAKEN 6.017 6.457 7.057 SPILLED NOT TAKEN 5.871 6.382 7.090 6.403 NOT TAKEN 8.078 5.870 7.275 6.455 NOT TAKEN 6.177 5.144 6.413 NOT TAKEN 5.301 5.724 SPILLED NOT TAKEN 4.772 5.694 5.027 5.625 5.357 5.758 5.793 NOT TAKEN 5.345 5.951 6.996 SPILLED NOT TAKEN 5.797 5.477 5.674 5.843 NOT TAKEN 7.435 5.242 6.226 6.140 NOT TAKEN 5.795 4.718 5.802 NOT TAKEN 5.095 5.438 SPILLED NOT TAKEN 5.542 5.728 5.716 6.078 5.297 5.957 3.641 NOT TAKEN 5.072 6.175 7.242 SPILLED NOT TAKEN 4.979 5.703 6.234 5.462 NOT TAKEN 6.591 5.205 6.296 5.575 NOT TAKEN 5.314 4.629 5.906 NOT TAKEN 4.675 5.029 SPILLED NOT TAKEN 5.219 5.459 5.641 6.028 5.369 5.818 4.441 NOT TAKEN 4.802 5.906 6.811 SPILLED NOT TAKEN 5.294 5.491 5.627 5.472 NOT TAKEN SPILLED 5.299 5.949 4.503 NOT TAKEN 5.672 4.533 5.846 NOT TAKEN 4.346 4.521 SPILLED NOT TAKEN 4.667 5.122 5.052 5.787 4.270 NOT TAKEN 4.556 NOT TAKEN 4.871 NOT TAKEN NOT TAKEN 8.024 NOT TAKEN 4.897 NOT TAKEN NOT TAKEN 5.332 NOT TAKEN 7.176 5.041 5.366 5.712 NOT TAKEN 5.234 4.468 SPILLED NOT TAKEN 4.096 5.054 SPILLED NOT TAKEN 5.103 5.014 5.113 5.976 4.831 NOT TAKEN 5.006 NOT TAKEN 4.451 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 5.045 NOT TAKEN NOT TAKEN 5.013 NOT TAKEN 6.710 5.155 5.379 NOT TAKEN NOT TAKEN 4.687 4.034 5.683 NOT TAKEN 4.244 4.839 SPILLED NOT TAKEN 5.083 5.310 4.416 5.518 5.106 NOT TAKEN 4.463 NOT TAKEN 4.135 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.640 NOT TAKEN NOT TAKEN 4.375 NOT TAKEN 5.539 5.263 4.992 NOT TAKEN NOT TAKEN 4.819 4.106 5.937 NOT TAKEN 4.429 4.823 9.520 NOT TAKEN 5.637 5.209 4.852 5.696 4.953 NOT TAKEN 4.802 NOT TAKEN 3.742 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.576 NOT TAKEN NOT TAKEN 4.673 NOT TAKEN 5.978 4.880 4.724 NOT TAKEN NOT TAKEN 4.553 3.885 5.650 NOT TAKEN 4.706 5.160 SPILLED NOT TAKEN 5.117 5.033 4.389 5.667 4.074 NOT TAKEN 4.629 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.681 NOT TAKEN NOT TAKEN 5.143 NOT TAKEN 5.211 5.334 5.166 1438 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 56 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91359 C91360 C91361 C91362 C91363 C91364 C91365 C91366 C91367 C91368 C91369 C91370 C91371 C91372 C91373 C91374 C91375 C91376 C91377 C91378 C91379 C91380 6.760 6.999 5.227 5.875 6.081 5.943 SPILLED 5.898 7.080 6.113 6.080 NOT TAKEN 6.038 5.429 6.868 5.487 5.692 5.986 6.223 5.794 NOT TAKEN 5.664 8.135 7.732 5.447 6.547 5.679 6.650 SPILLED 6.333 7.140 6.569 6.929 NOT TAKEN 6.465 5.821 7.308 5.912 6.397 6.344 5.755 6.385 NOT TAKEN 6.384 6.376 6.747 5.327 5.859 5.290 5.633 10.673 5.493 5.879 5.582 5.710 NOT TAKEN 5.804 4.939 6.479 5.341 5.847 5.807 4.945 5.172 NOT TAKEN 5.899 6.180 6.306 5.446 5.160 5.229 5.649 11.678 5.513 5.628 6.231 5.956 NOT TAKEN 7.947 5.191 6.317 5.137 6.034 5.785 5.580 5.597 NOT TAKEN 6.055 5.954 6.182 5.195 5.315 4.976 5.555 10.829 5.507 6.035 6.474 5.530 NOT TAKEN 5.582 4.677 6.720 5.303 5.732 5.536 5.360 5.592 NOT TAKEN 5.710 5.641 5.942 5.471 4.421 4.816 5.275 NOT TAKEN 5.116 NOT TAKEN NOT TAKEN 5.402 NOT TAKEN NOT TAKEN 4.531 5.749 4.447 5.173 5.400 5.239 6.696 NOT TAKEN 5.745 5.308 6.010 5.519 4.577 4.903 5.400 NOT TAKEN 5.114 NOT TAKEN NOT TAKEN 5.659 NOT TAKEN NOT TAKEN 4.814 5.684 4.340 5.452 5.250 4.536 6.604 NOT TAKEN 5.533 5.035 5.599 5.231 4.782 4.750 4.783 NOT TAKEN 4.471 NOT TAKEN NOT TAKEN 5.009 NOT TAKEN NOT TAKEN 4.318 5.033 4.405 4.991 5.184 4.082 7.931 NOT TAKEN 5.225 5.578 5.512 4.740 4.896 4.644 5.094 NOT TAKEN 4.659 NOT TAKEN NOT TAKEN 5.362 NOT TAKEN NOT TAKEN 4.621 0.106 4.457 5.646 3.368 4.227 7.810 NOT TAKEN 5.406 5.692 5.389 4.605 4.728 4.636 5.191 NOT TAKEN 4.885 NOT TAKEN NOT TAKEN 5.160 NOT TAKEN NOT TAKEN 4.406 NOT TAKEN 4.441 5.404 5.347 NOT TAKEN SPILLED NOT TAKEN 5.563 1439 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 57 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 100 PPM C91451 C91452 C91453 C91454 C91455 C91456 C91457 C91458 C91459 C91460 C91461 C91462 C91463 C91464 C91465 C91466 C91467 C91468 C91469 C91470 C91471 C91472 C91473 C91474 C91475 C91476 7.546 6.048 6.288 6.105 6.200 5.712 5.827 6.380 SPILLED 6.228 4.868 5.760 6.151 6.444 6.634 5.193 6.450 SPILLED 6.067 6.307 6.143 5.415 5.937 6.547 6.474 6.303 6.725 6.491 6.862 6.330 6.394 6.548 6.303 6.868 SPILLED 6.956 5.558 6.458 6.348 7.186 7.466 6.089 7.310 SPILLED 6.469 6.919 6.796 5.910 7.451 7.305 6.751 6.754 5.866 5.883 5.923 5.704 5.745 5.659 5.599 5.973 9.704 5.746 4.994 5.729 5.462 6.253 6.629 4.929 6.628 7.736 5.624 5.626 5.729 5.206 5.341 5.422 6.054 5.833 5.549 5.374 5.400 5.465 5.671 5.502 5.257 6.142 SPILLED 5.711 4.900 5.193 5.546 6.472 6.182 5.043 6.562 6.463 5.562 5.913 5.661 5.266 5.162 5.817 5.860 5.875 5.721 5.170 5.281 5.329 5.400 5.334 5.026 5.518 SPILLED 5.040 4.489 5.087 5.530 6.484 5.836 4.992 6.196 6.992 5.710 5.253 5.099 4.557 5.513 5.525 5.437 5.401 1440 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 58 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 100 PPM C91477 C91478 C91479 C91480 C91481 C91482 C91483 C91484 C91485 C91486 C91487 C91488 C91489 C91490 6.347 6.328 5.970 6.481 5.905 6.231 6.163 6.023 6.196 6.293 5.744 5.554 6.367 SPILLED 6.578 6.855 6.891 6.844 6.613 6.701 6.510 5.815 6.548 6.332 6.505 5.672 6.950 6.926 5.809 6.338 5.681 6.362 5.907 5.592 5.597 5.908 5.913 5.878 5.407 5.189 5.867 6.031 5.759 6.547 5.222 6.188 5.957 6.022 5.568 5.396 5.456 5.988 5.865 5.284 6.073 6.002 5.181 5.813 5.327 5.442 5.639 5.478 3.792 5.128 4.401 5.632 5.354 4.647 5.583 5.507 1441 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 59 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 3 PPM C90781 C90782 C90783 C90784 C90785 C90786 C90787 C90788 C90789 C90790 C90791 C90792 C90793 C90794 C90795 C90796 C90797 C90798 C90799 C90800 C90801 C90802 C90803 C90804 C90805 C90806 C90807 C90808 C90809 C90810 C90811 0.100 0.103 0.109 0.101 0.100 0.102 0.101 0.090 NOT TAKEN 0.072 0.111 NOT TAKEN 0.081 0.065 NOT TAKEN NOT TAKEN NOT TAKEN 0.112 0.111 0.104 0.100 0.089 0.070 0.097 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.101 SPILLED 0.105 0.099 0.094 0.111 0.107 0.103 0.111 0.104 0.088 NOT TAKEN 0.039 0.116 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.114 0.107 0.107 SPILLED 0.003 0.124 0.103 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.098 0.104 0.098 SPILLED 0.056 0.107 0.118 0.109 0.097 0.096 NOT TAKEN NOT TAKEN 0.112 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.108 0.101 NOT TAKEN 0.128 NOT TAKEN 0.191 0.095 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.097 0.100 0.094 0.092 0.106 0.094 0.110 0.096 0.093 0.082 NOT TAKEN NOT TAKEN 0.103 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.086 0.091 NOT TAKEN 0.112 NOT TAKEN 0.194 0.090 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.088 0.091 0.093 0.098 0.102 0.074 0.117 0.095 0.084 0.084 NOT TAKEN NOT TAKEN 0.105 NOT TAKEN NOT TAKEN 0.113 NOT TAKEN NOT TAKEN NOT TAKEN 0.085 0.098 NOT TAKEN 0.113 NOT TAKEN SPILLED 0.089 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.085 0.096 0.092 0.079 0.093 NOT TAKEN 0.106 NOT TAKEN 0.091 0.077 NOT TAKEN NOT TAKEN 0.101 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.090 0.094 NOT TAKEN 0.105 NOT TAKEN NOT TAKEN 0.079 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.136 0.088 0.088 0.097 0.114 0.092 NOT TAKEN 0.083 NOT TAKEN 0.064 0.077 NOT TAKEN NOT TAKEN 0.093 NOT TAKEN NOT TAKEN 0.094 NOT TAKEN NOT TAKEN NOT TAKEN 0.020 0.096 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.063 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.114 0.083 0.092 0.085 0.112 0.086 NOT TAKEN 0.026 NOT TAKEN 0.094 0.075 NOT TAKEN NOT TAKEN 0.092 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.090 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.051 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.079 0.090 1442 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 60 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 3 PPM C90812 C90813 C90814 C90815 C90816 C90817 C90818 C90819 C90820 C90821 C90822 C90823 C90824 C90825 C90826 C90827 C90828 C90829 C90830 C90831 C90832 C90833 C90834 C90835 C90836 C90837 C90838 C90839 C90840 0.107 NOT TAKEN NOT TAKEN 0.105 0.086 NOT TAKEN NOT TAKEN 0.089 NOT TAKEN 0.096 0.000 0.095 0.102 0.102 0.116 NOT TAKEN 0.112 0.097 0.102 NOT TAKEN 0.105 NOT TAKEN NOT TAKEN 0.116 NOT TAKEN NOT TAKEN 0.092 0.086 0.086 0.107 NOT TAKEN NOT TAKEN 0.100 0.097 NOT TAKEN NOT TAKEN 0.102 NOT TAKEN 0.096 NOT TAKEN 0.097 0.015 0.101 0.114 NOT TAKEN NOT TAKEN 0.088 0.102 NOT TAKEN 0.086 NOT TAKEN NOT TAKEN 0.119 NOT TAKEN NOT TAKEN 0.090 0.081 SPILLED 0.109 NOT TAKEN NOT TAKEN 0.097 0.027 NOT TAKEN NOT TAKEN 0.100 NOT TAKEN 0.098 NOT TAKEN 0.103 NOT TAKEN 0.105 0.121 NOT TAKEN NOT TAKEN 0.092 0.110 NOT TAKEN 0.112 NOT TAKEN NOT TAKEN 0.103 NOT TAKEN NOT TAKEN 0.091 0.119 SPILLED 0.104 NOT TAKEN NOT TAKEN 0.090 NOT TAKEN NOT TAKEN NOT TAKEN 0.087 NOT TAKEN 0.092 NOT TAKEN 0.087 NOT TAKEN 0.099 0.102 NOT TAKEN NOT TAKEN 0.082 0.090 NOT TAKEN 0.094 NOT TAKEN NOT TAKEN 0.114 NOT TAKEN NOT TAKEN 0.086 SPILLED SPILLED 0.099 NOT TAKEN NOT TAKEN 0.095 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.091 NOT TAKEN 0.073 NOT TAKEN 0.066 NOT TAKEN NOT TAKEN NOT TAKEN 0.078 0.095 NOT TAKEN 0.080 NOT TAKEN NOT TAKEN 0.112 NOT TAKEN NOT TAKEN 0.079 0.080 SPILLED 0.084 NOT TAKEN NOT TAKEN 0.080 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.085 NOT TAKEN 0.086 NOT TAKEN 0.095 NOT TAKEN NOT TAKEN NOT TAKEN 0.077 0.082 NOT TAKEN 0.096 NOT TAKEN NOT TAKEN 0.100 NOT TAKEN NOT TAKEN 0.085 0.095 SPILLED 0.076 NOT TAKEN NOT TAKEN 0.063 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.089 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.087 0.088 NOT TAKEN 0.098 NOT TAKEN NOT TAKEN 0.094 NOT TAKEN NOT TAKEN 0.086 NOT TAKEN SPILLED 0.078 NOT TAKEN NOT TAKEN 0.076 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.096 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.059 0.078 NOT TAKEN 0.090 NOT TAKEN NOT TAKEN 0.101 NOT TAKEN NOT TAKEN 0.083 NOT TAKEN SPILLED 1443 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 61 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90841 C90842 C90843 C90844 C90845 C90846 C90847 C90848 C90849 C90850 C90851 C90852 C90853 C90854 C90855 C90856 C90857 C90858 C90859 C90860 C90861 C90862 C90863 C90864 C90865 C90866 C90867 C90868 C90869 C90870 C90871 1.058 1.026 NOT TAKEN 1.173 0.644 NOT TAKEN 1.101 NOT TAKEN 0.669 1.025 1.065 NOT TAKEN NOT TAKEN 1.117 0.948 0.947 1.064 0.955 NOT TAKEN 1.306 NOT TAKEN NOT TAKEN NOT TAKEN 0.984 1.095 NOT TAKEN 1.324 NOT TAKEN 1.033 0.726 0.901 1.089 1.065 NOT TAKEN 1.105 NOT TAKEN NOT TAKEN 1.108 NOT TAKEN NOT TAKEN 1.066 1.021 NOT TAKEN NOT TAKEN 1.079 1.028 0.978 1.117 1.123 NOT TAKEN 1.249 NOT TAKEN NOT TAKEN NOT TAKEN 1.062 1.059 NOT TAKEN 1.404 NOT TAKEN 1.019 NOT TAKEN 1.081 0.975 1.071 NOT TAKEN 1.142 NOT TAKEN NOT TAKEN 0.871 NOT TAKEN NOT TAKEN 1.007 1.016 NOT TAKEN NOT TAKEN 1.046 0.956 1.009 1.212 1.000 NOT TAKEN 1.275 NOT TAKEN NOT TAKEN NOT TAKEN 1.048 1.068 NOT TAKEN 1.286 NOT TAKEN 1.050 NOT TAKEN 0.995 0.850 0.866 NOT TAKEN 1.055 NOT TAKEN NOT TAKEN 0.854 NOT TAKEN NOT TAKEN 0.995 0.895 NOT TAKEN NOT TAKEN 0.938 0.856 0.893 1.087 0.928 NOT TAKEN 1.112 NOT TAKEN NOT TAKEN NOT TAKEN 0.945 1.007 NOT TAKEN 1.231 NOT TAKEN 0.975 NOT TAKEN 0.928 0.664 0.904 NOT TAKEN 1.048 NOT TAKEN NOT TAKEN 0.748 NOT TAKEN NOT TAKEN 0.788 0.934 NOT TAKEN NOT TAKEN 0.887 0.889 0.899 1.061 0.800 NOT TAKEN 1.073 NOT TAKEN NOT TAKEN NOT TAKEN 0.943 0.967 NOT TAKEN 1.174 NOT TAKEN 0.892 NOT TAKEN 0.941 0.317 0.881 NOT TAKEN 1.009 NOT TAKEN NOT TAKEN 0.249 NOT TAKEN NOT TAKEN 0.613 0.966 NOT TAKEN NOT TAKEN 0.851 0.821 0.826 0.875 SPILLED NOT TAKEN 1.066 NOT TAKEN NOT TAKEN NOT TAKEN 0.872 0.861 NOT TAKEN 1.112 NOT TAKEN 0.816 NOT TAKEN 0.819 NOT TAKEN 0.949 NOT TAKEN 0.859 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.949 0.925 NOT TAKEN NOT TAKEN 0.868 0.840 0.735 0.654 1.111 NOT TAKEN 1.062 NOT TAKEN NOT TAKEN NOT TAKEN 0.956 0.875 NOT TAKEN 1.170 NOT TAKEN 0.770 NOT TAKEN 0.887 NOT TAKEN 0.994 NOT TAKEN 0.747 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.815 0.876 NOT TAKEN NOT TAKEN 0.617 0.865 0.815 NOT TAKEN 0.811 NOT TAKEN 1.119 NOT TAKEN NOT TAKEN NOT TAKEN 0.978 0.840 NOT TAKEN 1.192 NOT TAKEN 0.888 NOT TAKEN 0.889 1444 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 62 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 30 PPM C90872 C90873 C90874 C90875 C90876 C90877 C90878 C90879 C90880 C90881 C90882 C90883 C90884 C90885 C90886 C90887 C90888 C90889 C90890 C90891 C90892 C90893 C90894 C90895 C90896 C90897 C90898 C90899 C90900 1.112 NOT TAKEN NOT TAKEN 1.020 NOT TAKEN NOT TAKEN NOT TAKEN 0.788 NOT TAKEN 1.043 0.995 1.186 1.414 0.979 1.092 NOT TAKEN 0.983 1.063 NOT TAKEN NOT TAKEN 0.010 1.040 1.026 1.029 NOT TAKEN 1.008 NOT TAKEN 1.148 1.072 1.095 NOT TAKEN NOT TAKEN 1.017 NOT TAKEN NOT TAKEN NOT TAKEN 0.334 NOT TAKEN 1.017 1.059 1.139 SPILLED 0.902 0.869 NOT TAKEN 0.009 0.973 NOT TAKEN NOT TAKEN NOT TAKEN 1.047 0.981 1.065 NOT TAKEN 0.975 NOT TAKEN 1.104 1.095 1.150 NOT TAKEN NOT TAKEN 1.038 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.979 1.052 1.400 1.306 0.933 0.590 NOT TAKEN NOT TAKEN 1.106 NOT TAKEN NOT TAKEN NOT TAKEN 0.990 1.035 1.046 NOT TAKEN 1.022 NOT TAKEN 1.100 1.039 0.784 NOT TAKEN NOT TAKEN 0.973 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.974 0.954 1.106 1.169 0.782 0.670 NOT TAKEN NOT TAKEN 0.983 NOT TAKEN NOT TAKEN NOT TAKEN 0.939 0.881 0.954 NOT TAKEN 0.938 NOT TAKEN 1.134 0.976 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.962 0.986 1.042 1.279 0.966 NOT TAKEN NOT TAKEN NOT TAKEN 1.008 NOT TAKEN NOT TAKEN NOT TAKEN 0.965 0.872 0.905 NOT TAKEN 0.952 NOT TAKEN 1.032 1.027 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.858 0.876 1.188 1.091 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.905 NOT TAKEN NOT TAKEN NOT TAKEN 0.922 0.835 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.918 0.952 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.887 0.820 0.907 1.062 1.087 NOT TAKEN NOT TAKEN NOT TAKEN 0.835 NOT TAKEN NOT TAKEN NOT TAKEN 0.883 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.623 0.906 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.840 0.827 1.051 0.570 0.811 NOT TAKEN NOT TAKEN NOT TAKEN 0.839 NOT TAKEN NOT TAKEN NOT TAKEN 0.884 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.957 1445 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 63 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90901 C90902 C90903 C90904 C90905 C90906 C90907 C90908 C90909 C90910 C90911 C90912 C90913 C90914 C90915 C90916 C90917 C90918 C90919 C90920 C90921 C90922 C90923 C90924 C90925 C90926 C90927 C90928 C90929 C90930 C90931 NOT TAKEN NOT TAKEN 3.400 3.836 NOT TAKEN 3.122 NOT TAKEN 3.597 NOT TAKEN 3.316 3.451 3.395 NOT TAKEN 3.430 3.585 3.836 NOT TAKEN NOT TAKEN 3.611 4.025 NOT TAKEN NOT TAKEN 4.148 NOT TAKEN 3.909 3.489 3.740 3.602 NOT TAKEN 3.512 3.694 NOT TAKEN NOT TAKEN 3.374 3.813 NOT TAKEN 3.437 NOT TAKEN 3.585 NOT TAKEN 3.473 3.723 2.930 NOT TAKEN 3.481 3.530 3.749 NOT TAKEN NOT TAKEN NOT TAKEN 4.026 NOT TAKEN NOT TAKEN 3.214 NOT TAKEN 3.712 3.449 3.693 3.776 NOT TAKEN 3.768 3.604 NOT TAKEN NOT TAKEN 3.591 3.708 NOT TAKEN 3.308 NOT TAKEN 3.829 NOT TAKEN 3.543 3.607 NOT TAKEN NOT TAKEN 3.239 3.366 3.580 NOT TAKEN NOT TAKEN NOT TAKEN 3.926 NOT TAKEN NOT TAKEN 1.397 NOT TAKEN 3.397 3.396 3.564 0.811 NOT TAKEN 3.758 3.005 NOT TAKEN NOT TAKEN 3.085 3.413 NOT TAKEN 3.340 NOT TAKEN 3.259 NOT TAKEN 3.033 3.274 NOT TAKEN NOT TAKEN 2.570 3.247 3.096 NOT TAKEN NOT TAKEN NOT TAKEN 3.313 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 2.998 2.700 3.373 1.802 NOT TAKEN 3.911 3.318 NOT TAKEN NOT TAKEN 3.023 3.122 NOT TAKEN 3.369 NOT TAKEN 3.152 NOT TAKEN 3.319 3.095 NOT TAKEN NOT TAKEN 2.256 3.072 3.211 NOT TAKEN NOT TAKEN NOT TAKEN 3.875 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.563 3.293 2.756 4.520 NOT TAKEN NOT TAKEN 3.275 NOT TAKEN NOT TAKEN 2.914 3.169 NOT TAKEN 3.254 NOT TAKEN SPILLED NOT TAKEN 2.284 3.002 NOT TAKEN NOT TAKEN 1.649 NOT TAKEN 2.961 NOT TAKEN NOT TAKEN NOT TAKEN 3.785 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.930 2.962 3.615 NOT TAKEN NOT TAKEN 2.572 NOT TAKEN NOT TAKEN 2.440 3.301 NOT TAKEN 2.042 NOT TAKEN 3.180 NOT TAKEN 3.206 3.229 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 2.973 NOT TAKEN NOT TAKEN NOT TAKEN 3.121 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 3.202 3.643 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 2.915 NOT TAKEN NOT TAKEN NOT TAKEN 2.873 NOT TAKEN 3.055 3.171 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 2.941 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 3.110 4.724 NOT TAKEN NOT TAKEN NOT TAKEN 1446 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 64 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90932 C90933 C90934 C90935 C90936 C90937 C90938 C90939 C90940 C90941 C90942 C90943 C90944 C90945 C90946 C90947 C90948 C90949 C90950 C90951 C90952 C90953 C90954 C90955 C90956 C90957 C90958 C90959 C90960 C90961 C90962 NOT TAKEN 3.588 4.069 NOT TAKEN 4.115 4.219 NOT TAKEN NOT TAKEN NOT TAKEN 3.007 NOT TAKEN 3.157 3.687 NOT TAKEN 2.223 3.721 3.994 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.550 NOT TAKEN 3.471 3.868 NOT TAKEN 1.121 3.396 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.236 SPILLED NOT TAKEN SPILLED 4.148 NOT TAKEN NOT TAKEN NOT TAKEN 2.096 NOT TAKEN SPILLED 3.821 NOT TAKEN NOT TAKEN 3.393 4.342 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.469 NOT TAKEN 3.837 3.968 NOT TAKEN 3.261 3.681 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.219 3.928 NOT TAKEN 3.949 4.297 NOT TAKEN NOT TAKEN NOT TAKEN 1.865 NOT TAKEN 3.844 3.750 NOT TAKEN NOT TAKEN 3.697 3.796 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.383 NOT TAKEN 3.715 3.623 NOT TAKEN SPILLED 2.549 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.817 3.776 NOT TAKEN 3.713 3.765 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 2.059 3.576 NOT TAKEN NOT TAKEN 3.807 3.257 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.064 NOT TAKEN 3.311 3.346 NOT TAKEN 3.521 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.204 3.406 NOT TAKEN 4.001 3.415 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.284 NOT TAKEN NOT TAKEN 3.655 3.450 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.148 NOT TAKEN 3.385 3.081 NOT TAKEN 2.898 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.605 3.286 NOT TAKEN 3.589 3.342 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.284 NOT TAKEN NOT TAKEN 3.881 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 2.821 NOT TAKEN 2.903 2.835 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.223 2.523 NOT TAKEN 2.686 3.239 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.526 NOT TAKEN NOT TAKEN 4.149 3.386 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.009 NOT TAKEN 2.977 2.781 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 2.777 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.169 NOT TAKEN NOT TAKEN 4.503 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.023 NOT TAKEN 2.975 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1447 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 65 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 100 PPM C90963 C90964 C90965 C90966 C90967 C90968 C90969 C90970 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 3.724 3.497 3.473 NOT TAKEN NOT TAKEN 4.972 NOT TAKEN NOT TAKEN SPILLED 3.958 3.508 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 1.781 3.228 3.585 NOT TAKEN NOT TAKEN 4.747 NOT TAKEN NOT TAKEN 3.413 NOT TAKEN 2.943 NOT TAKEN NOT TAKEN 4.533 NOT TAKEN NOT TAKEN 2.312 NOT TAKEN 3.231 NOT TAKEN NOT TAKEN 4.151 NOT TAKEN NOT TAKEN 2.419 NOT TAKEN 3.351 NOT TAKEN NOT TAKEN 4.503 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.424 NOT TAKEN NOT TAKEN 3.372 NOT TAKEN NOT TAKEN 1448 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 66 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91191 C91192 C91193 C91194 C91195 C91196 C91197 C91198 C91199 C91200 C91201 C91202 C91203 C91204 C91205 C91206 C91207 C91208 C91209 0.118 NOT TAKEN 0.111 0.159 0.124 0.105 0.114 NOT TAKEN 0.125 0.143 0.143 0.125 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.144 NOT TAKEN NOT TAKEN 0.115 NOT TAKEN SPILLED SPILLED 0.122 0.018 0.129 NOT TAKEN 0.143 0.147 0.142 0.123 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.158 NOT TAKEN NOT TAKEN 0.112 NOT TAKEN 0.129 0.144 0.126 NOT TAKEN 0.126 NOT TAKEN 0.124 0.140 0.129 0.080 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.144 NOT TAKEN NOT TAKEN 0.115 NOT TAKEN 0.067 0.122 0.109 NOT TAKEN 0.124 NOT TAKEN 0.131 NOT TAKEN 0.135 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.135 NOT TAKEN NOT TAKEN 0.133 NOT TAKEN NOT TAKEN 0.130 0.078 NOT TAKEN 0.123 NOT TAKEN 0.079 NOT TAKEN 0.125 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.048 NOT TAKEN NOT TAKEN 0.075 NOT TAKEN NOT TAKEN 0.112 0.049 NOT TAKEN 0.109 NOT TAKEN 0.117 NOT TAKEN 0.089 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.144 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.060 NOT TAKEN NOT TAKEN 0.103 NOT TAKEN NOT TAKEN NOT TAKEN 0.064 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.133 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.107 NOT TAKEN NOT TAKEN NOT TAKEN 0.132 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.140 NOT TAKEN NOT TAKEN 1449 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 67 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91210 C91211 C91212 C91213 C91214 C91215 C91216 C91217 C91218 C91219 C91220 C91221 C91222 C91223 C91224 C91225 C91226 C91227 C91228 C91229 C91230 C91231 C91232 C91233 C91234 C91235 C91236 C91237 C91238 C91239 C91240 NOT TAKEN 0.118 NOT TAKEN 0.122 0.145 NOT TAKEN 0.184 NOT TAKEN 0.196 0.162 0.140 0.132 NOT TAKEN NOT TAKEN 0.135 0.138 0.110 0.136 0.182 0.099 NOT TAKEN 0.117 0.153 NOT TAKEN NOT TAKEN SPILLED 0.166 NOT TAKEN NOT TAKEN NOT TAKEN 0.146 NOT TAKEN 0.128 NOT TAKEN NOT TAKEN 0.142 NOT TAKEN 0.167 NOT TAKEN 0.140 0.135 0.137 0.130 NOT TAKEN NOT TAKEN 0.136 0.053 0.087 0.124 SPILLED 0.181 NOT TAKEN 0.123 0.162 NOT TAKEN NOT TAKEN SPILLED 0.178 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.120 NOT TAKEN NOT TAKEN 0.111 NOT TAKEN SPILLED NOT TAKEN 0.073 0.072 0.123 0.126 NOT TAKEN NOT TAKEN 0.144 NOT TAKEN 0.174 0.116 0.169 0.118 NOT TAKEN 0.123 0.160 NOT TAKEN NOT TAKEN SPILLED 0.173 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.120 NOT TAKEN NOT TAKEN 0.132 NOT TAKEN SPILLED NOT TAKEN 0.119 NOT TAKEN 0.102 0.123 NOT TAKEN NOT TAKEN 0.134 NOT TAKEN NOT TAKEN 0.098 0.101 0.117 NOT TAKEN 0.089 0.163 NOT TAKEN NOT TAKEN 0.130 0.130 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.128 NOT TAKEN NOT TAKEN 0.142 NOT TAKEN 0.147 NOT TAKEN 0.145 NOT TAKEN 0.127 0.117 NOT TAKEN NOT TAKEN 0.101 NOT TAKEN NOT TAKEN 0.105 0.084 0.036 NOT TAKEN NOT TAKEN 0.155 NOT TAKEN NOT TAKEN 0.135 0.140 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.113 NOT TAKEN NOT TAKEN 0.105 NOT TAKEN 0.158 NOT TAKEN SPILLED NOT TAKEN 0.115 0.118 NOT TAKEN NOT TAKEN 0.114 NOT TAKEN NOT TAKEN 0.109 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.136 NOT TAKEN NOT TAKEN 0.129 0.131 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.107 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.160 NOT TAKEN NOT TAKEN NOT TAKEN 0.096 0.101 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.106 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.142 NOT TAKEN NOT TAKEN 0.114 0.125 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.099 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.160 NOT TAKEN NOT TAKEN NOT TAKEN 0.082 0.091 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.109 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.119 NOT TAKEN NOT TAKEN 0.116 0.112 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1450 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 68 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 3 PPM C91241 C91242 C91243 C91244 C91245 C91246 C91247 C91248 C91249 C91250 NOT TAKEN SPILLED 0.147 NOT TAKEN 0.132 0.131 NOT TAKEN NOT TAKEN 0.122 NOT TAKEN NOT TAKEN SPILLED 0.143 NOT TAKEN 0.122 0.160 NOT TAKEN NOT TAKEN 0.117 NOT TAKEN NOT TAKEN SPILLED 0.148 NOT TAKEN 0.124 0.115 NOT TAKEN NOT TAKEN 0.109 NOT TAKEN NOT TAKEN 0.141 0.123 NOT TAKEN 0.119 NOT TAKEN NOT TAKEN NOT TAKEN 0.094 NOT TAKEN NOT TAKEN 0.129 0.114 NOT TAKEN 0.079 NOT TAKEN NOT TAKEN NOT TAKEN 0.015 NOT TAKEN NOT TAKEN 0.131 0.111 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.022 NOT TAKEN NOT TAKEN 0.120 0.090 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.124 0.091 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN GROUP: FEMALE 3 - 30 PPM C91251 C91252 C91253 C91254 C91255 C91256 C91257 C91258 C91259 C91260 C91261 C91262 C91263 C91264 C91265 C91266 C91267 C91268 NOT TAKEN 1.154 1.274 1.347 NOT TAKEN SPILLED 1.291 1.139 NOT TAKEN NOT TAKEN 1.283 1.410 NOT TAKEN 1.344 1.511 1.437 NOT TAKEN 1.187 NOT TAKEN 1.257 1.360 1.309 NOT TAKEN 1.699 1.319 1.558 NOT TAKEN NOT TAKEN 1.342 1.443 NOT TAKEN 1.634 1.362 1.279 NOT TAKEN 1.325 NOT TAKEN 1.165 1.350 1.405 NOT TAKEN 1.735 NOT TAKEN 0.137 NOT TAKEN NOT TAKEN 1.182 1.319 NOT TAKEN 1.478 1.360 1.332 NOT TAKEN 1.094 NOT TAKEN 1.128 1.206 1.344 NOT TAKEN 1.542 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.034 0.014 NOT TAKEN 1.374 1.100 1.188 NOT TAKEN 1.224 NOT TAKEN 1.158 1.025 1.156 NOT TAKEN 1.427 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.113 NOT TAKEN NOT TAKEN 1.305 0.480 1.085 NOT TAKEN 1.146 NOT TAKEN 0.983 1.044 1.374 NOT TAKEN 1.103 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.851 NOT TAKEN NOT TAKEN 1.183 NOT TAKEN 1.044 NOT TAKEN 0.973 NOT TAKEN 0.933 0.994 1.197 NOT TAKEN 1.111 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.071 NOT TAKEN 0.496 NOT TAKEN NOT TAKEN NOT TAKEN 0.957 1.014 1.372 NOT TAKEN 1.268 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.816 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1451 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 69 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 30 PPM C91269 C91270 C91271 C91272 C91273 C91274 C91275 C91276 C91277 C91278 C91279 C91280 C91281 C91282 C91283 C91284 C91285 C91286 C91287 C91288 C91289 C91290 C91291 C91292 C91293 C91294 C91295 C91296 C91297 C91298 C91299 0.509 NOT TAKEN 1.471 NOT TAKEN 1.616 NOT TAKEN NOT TAKEN 1.556 NOT TAKEN 1.427 1.403 1.414 NOT TAKEN NOT TAKEN 1.249 1.316 NOT TAKEN 1.592 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.170 NOT TAKEN 1.617 1.309 1.599 1.396 NOT TAKEN NOT TAKEN 0.485 NOT TAKEN 1.131 NOT TAKEN 1.211 NOT TAKEN NOT TAKEN 1.801 NOT TAKEN 1.357 SPILLED 1.399 NOT TAKEN NOT TAKEN 1.458 1.387 NOT TAKEN 1.571 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.140 NOT TAKEN 1.602 1.470 1.037 1.492 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.923 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.622 NOT TAKEN 1.326 SPILLED 1.344 NOT TAKEN NOT TAKEN 1.186 1.344 NOT TAKEN 1.603 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.201 NOT TAKEN 1.544 1.277 1.027 1.397 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.268 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.577 NOT TAKEN 1.208 1.532 1.317 NOT TAKEN NOT TAKEN 0.021 1.204 NOT TAKEN 1.308 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.037 NOT TAKEN SPILLED 1.158 NOT TAKEN 1.574 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.610 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.447 NOT TAKEN 1.312 SPILLED 1.332 NOT TAKEN NOT TAKEN SPILLED 1.234 NOT TAKEN 1.375 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.061 NOT TAKEN 1.452 1.311 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.574 NOT TAKEN 1.266 1.184 0.717 NOT TAKEN NOT TAKEN NOT TAKEN 1.107 NOT TAKEN 1.347 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.158 NOT TAKEN 1.388 1.146 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.033 1.186 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.303 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.939 NOT TAKEN 1.296 1.054 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.101 1.156 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.241 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.966 NOT TAKEN 1.171 0.688 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1452 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 70 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 30 PPM C91300 C91301 C91302 C91303 C91304 C91305 C91306 C91307 C91308 C91309 C91310 1.609 1.111 NOT TAKEN 1.162 NOT TAKEN 1.226 1.590 1.518 NOT TAKEN NOT TAKEN 1.394 SPILLED 1.159 NOT TAKEN 1.148 NOT TAKEN 1.106 1.470 1.445 NOT TAKEN NOT TAKEN 1.432 NOT TAKEN 1.258 NOT TAKEN 1.057 NOT TAKEN 1.098 1.492 1.288 NOT TAKEN NOT TAKEN 1.448 NOT TAKEN 1.154 NOT TAKEN 0.904 NOT TAKEN NOT TAKEN 1.584 1.316 NOT TAKEN NOT TAKEN 1.340 NOT TAKEN 0.988 NOT TAKEN 1.073 NOT TAKEN NOT TAKEN 1.327 1.158 NOT TAKEN NOT TAKEN 1.139 NOT TAKEN 0.927 NOT TAKEN 0.905 NOT TAKEN NOT TAKEN 1.243 1.036 NOT TAKEN NOT TAKEN 1.198 NOT TAKEN 0.825 NOT TAKEN 0.805 NOT TAKEN NOT TAKEN 1.293 0.903 NOT TAKEN NOT TAKEN 1.214 NOT TAKEN 0.919 NOT TAKEN 0.887 NOT TAKEN NOT TAKEN NOT TAKEN 0.663 NOT TAKEN NOT TAKEN 1.210 GROUP: FEMALE 4 - 100 PPM C91311 C91312 C91313 C91314 C91315 C91316 C91317 C91318 C91319 C91320 C91321 C91322 C91323 C91324 C91325 C91326 C91327 NOT TAKEN 5.607 4.665 NOT TAKEN 5.272 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.179 4.895 NOT TAKEN NOT TAKEN 6.020 4.921 5.413 5.652 NOT TAKEN 5.508 4.980 NOT TAKEN 5.640 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.910 NOT TAKEN NOT TAKEN 5.883 5.638 4.964 5.630 NOT TAKEN 5.054 3.155 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.521 NOT TAKEN NOT TAKEN 5.576 4.780 5.509 5.666 NOT TAKEN 4.772 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.429 NOT TAKEN NOT TAKEN 4.321 4.607 NOT TAKEN 4.769 NOT TAKEN 4.349 NOT TAKEN NOT TAKEN 4.906 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.414 NOT TAKEN NOT TAKEN 5.181 4.234 NOT TAKEN 5.361 NOT TAKEN 1.204 NOT TAKEN NOT TAKEN 4.704 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.641 3.528 NOT TAKEN 4.677 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.552 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.919 2.077 NOT TAKEN 4.196 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.102 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.451 NOT TAKEN NOT TAKEN 3.803 1453 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 71 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91328 C91329 C91330 C91331 C91332 C91333 C91334 C91335 C91336 C91337 C91338 C91339 C91340 C91341 C91342 C91343 C91344 C91345 C91346 C91347 C91348 C91349 C91350 C91351 C91352 C91353 C91354 C91355 C91356 C91357 C91358 NOT TAKEN NOT TAKEN 4.909 1.577 8.815 NOT TAKEN 4.748 4.938 SPILLED NOT TAKEN 5.327 5.381 4.894 5.767 4.815 NOT TAKEN 4.958 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.206 NOT TAKEN NOT TAKEN 5.533 NOT TAKEN 6.264 5.199 NOT TAKEN NOT TAKEN NOT TAKEN 3.707 NOT TAKEN SPILLED NOT TAKEN 4.963 5.278 SPILLED NOT TAKEN 5.224 5.762 5.416 5.314 4.582 NOT TAKEN 5.446 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.406 NOT TAKEN NOT TAKEN 5.472 NOT TAKEN 6.306 5.060 NOT TAKEN NOT TAKEN NOT TAKEN 2.034 NOT TAKEN 6.333 NOT TAKEN 4.469 5.079 SPILLED NOT TAKEN 5.631 5.057 4.897 5.778 4.907 NOT TAKEN 4.483 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.831 NOT TAKEN NOT TAKEN 5.711 NOT TAKEN 5.418 4.820 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.840 NOT TAKEN 4.447 4.675 SPILLED NOT TAKEN 5.341 4.533 4.774 5.539 3.818 NOT TAKEN 4.355 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.888 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.793 4.583 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.953 NOT TAKEN 3.777 4.467 9.324 NOT TAKEN 4.779 2.935 4.794 4.997 4.202 NOT TAKEN 3.786 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.996 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.616 4.261 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 5.772 NOT TAKEN 4.426 3.973 SPILLED NOT TAKEN 4.337 4.329 4.523 5.301 4.152 NOT TAKEN 2.612 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.568 4.472 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.228 NOT TAKEN 6.315 3.580 8.380 NOT TAKEN 4.655 NOT TAKEN 3.578 4.614 3.868 NOT TAKEN 3.636 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 5.619 4.291 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.554 NOT TAKEN NOT TAKEN 3.832 SPILLED NOT TAKEN 3.730 NOT TAKEN 6.725 5.326 3.815 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.469 4.049 NOT TAKEN 1454 Appendix 4 Covance 6329-212 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 72 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 100 PPM C91359 C91360 C91361 C91362 C91363 C91364 C91365 C91366 C91367 C91368 C91369 C91370 C91371 C91372 C91373 C91374 C91375 C91376 C91377 C91378 C91379 C91380 5.387 5.745 5.029 4.507 4.608 4.923 NOT TAKEN 4.338 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.549 NOT TAKEN 4.887 5.701 5.011 NOT TAKEN 7.449 NOT TAKEN 5.492 NOT TAKEN 6.174 4.834 4.396 4.098 5.328 NOT TAKEN 4.485 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.796 NOT TAKEN 4.976 3.202 5.457 NOT TAKEN SPILLED NOT TAKEN 5.575 NOT TAKEN 5.689 4.611 4.629 4.900 4.681 NOT TAKEN 4.460 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.891 NOT TAKEN 4.945 NOT TAKEN 5.338 NOT TAKEN SPILLED NOT TAKEN 6.558 NOT TAKEN 4.839 4.119 4.187 4.413 4.436 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.053 NOT TAKEN 4.266 NOT TAKEN 4.796 NOT TAKEN 7.824 NOT TAKEN 5.383 NOT TAKEN 5.108 4.418 3.599 4.000 5.325 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.206 NOT TAKEN 2.882 NOT TAKEN 4.909 NOT TAKEN 8.874 NOT TAKEN 5.884 NOT TAKEN 4.921 4.177 3.531 SPILLED 5.001 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.622 NOT TAKEN 1.436 NOT TAKEN 4.769 NOT TAKEN SPILLED NOT TAKEN 6.438 NOT TAKEN 4.791 4.324 3.279 NOT TAKEN 7.263 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.919 NOT TAKEN NOT TAKEN NOT TAKEN 5.007 NOT TAKEN 8.630 NOT TAKEN 6.054 NOT TAKEN 4.639 3.806 3.052 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.703 NOT TAKEN NOT TAKEN NOT TAKEN 4.461 NOT TAKEN 5.823 NOT TAKEN 6.587 1455 APPENDIX 5 Individual Clinical Hematology Data Individual Clinical Chemistry Data Individual Clinical Urinalysis Data Individual Urine Chemistry Data Covance 6329-212 3M T-6316.1 1456 Appendix 5 Individual Clinical Hematology Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90717 C90718 C90737 C90756 C90769 8.08 8.58 7.91 8.39 8.37 15.4 15.5 14.5 15.6 15.3 45.1 46.6 42.6 44.5 44.8 55.9 54.2 53.9 53.0 53.5 19.1 18.1 18.3 18.6 18.2 34.1 33.4 34.0 35.1 34.1 1302 1300 1407 1106 1229 MEAN S.D. N 8.27 .267 5 15.3 .44 5 44.7 1.43 5 54.1 1.10 5 18.5 .40 5 34.1 .61 5 1269 111.0 5 Group: 5 Dose Level: 300 Dosage Unit: ppm C90971 C90972 C90988 C90996 C91017 7.34 6.28 6.35 5.93 7.17 13.4 12.2 12.3 12.0 13.4 38.3 36.2 35.6 35.2 40.3 52.1 57.7 56.1 59.2 56.2 18.2 19.4 19.5 20.3 18.6 34.9 33.6 34.7 34.2 33.2 1279 1042 989 1042 1106 MEAN S.D. N 6.61 .609 5 12.7 .68 5 37.1 2.14 5 56.3 2.65 5 19.2 .82 5 34.1 .72 5 1092 112.7 5 Covance 6329-212 3M T-6316.1 1457 ANIMAL NUMBER ------ Group: 1 C90717 C90718 C90737 C90756 C90769 MEAN S.D. N Group: 5 C90971 C90972 C90988 C90996 C91017 MEAN S.D. N Appendix 5 Covance 6329-212 3M T-6316.1 Individual Clinical Hematology Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 0 Dosage Unit: ppm 7.9 1.1 5.9 .8 .1 .0 14 74 11 2 0 5.4 .7 4.2 .4 .1 .0 13 79 7 1 0 14.1 1.0 13.0 .0 .1 .0 7 92 0 1 0 7.4 1.8 5.0 .5 .2 .0 24 68 6 2 0 7.9 1.3 6.2 .4 .1 .0 16 78 5 1 0 8.5 1.2 6.9 .4 .1 .0 15 78 6 1 0 3.27 .41 3.52 .29 .04 .00 6.1 8.8 4.0 .5 .0 5 5 5 5 5 5 5 5 5 5 5 Dose Level: 300 Dosage Unit: ppm 7.3 .9 6.2 .2 .1 .0 12 84 2 1 0 5.2 .6 4.1 .4 .0 .0 12 80 7 1 0 8.5 .9 7.1 .5 .1 .0 11 83 6 1 0 7.7 1.0 6.7 .0 .0 .0 12 87 0 1 0 3.7 1.4 2.1 .2 .0 .0 38 57 4 0 0 6.5 1.0 5.2 .3 .0 .0 17 78 4 1 0 1.98 .29 2.10 .19 .05 .00 11.7 12.1 2.9 .4 .0 5 5 5 5 5 5 5 5 5 5 5 1458 Appendix 5 Individual Clinical Hematology Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90717 - - - - - C90718 - - - - - C90737 - - - - - C90756 - - - - - C90769 - - - - - Group: 5 Dose Level: 300 Dosage Unit: ppm C90971 - - - - - C90972 - - - - - C90988 - - - - - C90996 - - - - - C91017 - - - - - Covance 6329-212 3M T-6316.1 1459 Appendix 5 Individual Clinical Hematology Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91126 C91140 C91145 C91162 C91163 8.18 8.11 7.56 7.46 7.85 14.7 15.2 14.4 14.3 15.1 44.2 45.2 41.9 41.8 44.8 54.0 55.7 55.3 56.0 57.1 17.9 18.7 19.1 19.1 19.3 33.2 33.6 34.4 34.2 33.8 1027 1117 1205 1022 1199 MEAN S.D. N 7.83 .321 5 14.7 .40 5 43.6 1.62 5 55.6 1.13 5 18.8 .56 5 33.8 .48 5 1114 88.8 5 Group: 5 Dose Level: 300 Dosage Unit: ppm C91393 C91394 C91404 C91415 C91438 6.18 6.93 6.82 7.09 7.15 11.8 13.3 12.7 13.1 14.0 34.5 38.8 36.8 38.6 41.0 55.9 56.0 54.0 54.5 57.3 19.0 19.3 18.6 18.4 19.5 34.0 34.4 34.5 33.8 34.1 1077 1305 1049 1016 1102 MEAN S.D. N 6.83 .388 5 13.0 .81 5 37.9 2.43 5 55.5 1.31 5 19.0 .46 5 34.2 .29 5 1110 113.7 5 Covance 6329-212 3M T-6316.1 1460 ANIMAL NUMBER ------ Group: 1 C91126 C91140 C91145 C91162 C91163 MEAN S.D. N Group: 5 C91393 C91394 C91404 C91415 C91438 MEAN S.D. N Appendix 5 Covance 6329-212 3M T-6316.1 Individual Clinical Hematology Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 0 Dosage Unit: ppm 8.7 .4 8.0 .1 .1 .0 5 93 1 1 0 6.1 1.2 4.3 .3 .3 .0 20 71 4 5 0 11.6 .6 10.5 .4 .1 .0 5 90 3 1 0 4.0 .5 3.1 .3 .1 .0 14 78 7 2 0 8.0 .7 7.0 .3 .1 .0 8 87 3 1 0 7.7 .7 6.6 .3 .1 .0 10 84 4 2 0 2.85 .31 2.95 .11 .09 .00 6.5 9.1 2.2 1.7 .0 5 5 5 5 5 5 5 5 5 5 5 Dose Level: 300 Dosage Unit: ppm 5.9 .5 5.2 .2 .0 .0 8 88 3 1 0 5.2 1.1 3.9 .1 .0 .0 21 76 2 1 0 4.9 .5 4.2 .1 .0 .0 9 87 3 0 0 6.8 .3 6.2 .3 .0 .0 4 91 4 1 0 6.3 .5 5.4 .3 .0 .0 8 86 5 1 0 5.8 .6 5.0 .2 .0 .0 10 86 3 1 0 .78 .30 .93 .10 .00 .00 6.4 5.7 1.1 .4 .0 5 5 5 5 5 5 5 5 5 5 5 1461 Appendix 5 Individual Clinical Hematology Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91126 - - - - - C91140 - - - - - C91145 - - - - - C91162 - - - - - C91163 - - - - - Group: 5 Dose Level: 300 Dosage Unit: ppm C91393 - - - - - C91394 - - - - - C91404 - - - - - C91415 - - - - - C91438 - - - - - Covance 6329-212 3M T-6316.1 1462 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90716 C90731 C90738 C90745 C90746 C90748 C90760 C90766 C90768 C90780 8.83 8.25 8.60 7.97 7.83 8.98 8.50 8.70 9.00 8.71 15.6 15.3 15.3 14.9 14.3 15.5 15.8 15.3 16.0 15.2 45.8 44.9 45.9 45.2 42.5 46.3 46.1 46.4 47.5 46.5 51.9 54.4 53.4 56.7 54.2 51.5 54.3 53.3 52.8 53.3 17.6 18.6 17.7 18.7 18.2 17.2 18.6 17.6 17.7 17.4 34.0 34.1 33.2 32.9 33.6 33.4 34.3 33.1 33.6 32.7 1359 1257 1261 948 1312 1342 1081 1407 1061 1374 MEAN S.D. N 8.54 .403 10 15.3 .48 10 45.7 1.34 10 53.6 1.46 10 17.9 .55 10 33.5 .53 10 1240 156.0 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C90791 C90797 C90802 C90807 C90816 C90818 C90820 C90831 C90836 C90840 8.49 8.95 9.02 8.71 8.26 9.02 8.41 8.81 8.92 8.66 15.0 16.2 15.8 16.2 15.7 16.0 15.0 15.7 15.5 15.2 44.4 48.3 47.7 47.6 46.2 47.0 45.7 46.6 47.2 45.6 52.3 53.9 52.8 54.7 55.9 52.1 54.3 52.9 53.0 52.7 17.7 18.1 17.5 18.6 19.1 17.7 17.9 17.8 17.4 17.5 33.7 33.6 33.1 34.0 34.1 34.0 32.9 33.7 32.8 33.3 1112 1164 1168 972 1214 1134 1115 1181 1196 1141 MEAN S.D. N 8.72 .268 10 15.6 .45 10 46.6 1.17 10 53.5 1.21 10 17.9 .54 10 33.5 .47 10 1140 67.8 10 Covance 6329-212 3M T-6316.1 1463 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90843 C90851 C90852 C90856 C90863 C90877 C90880 C90888 C90892 C90897 8.86 8.57 8.56 8.66 8.06 7.96 8.13 8.64 8.53 7.34 15.2 15.3 15.6 15.6 14.5 14.8 14.7 15.6 15.6 13.4 44.1 45.6 45.9 46.1 43.9 43.7 43.9 46.2 46.2 40.2 49.8 53.3 53.6 53.2 54.4 54.9 54.0 53.5 54.1 54.8 17.1 17.9 18.2 18.0 18.0 18.6 18.0 18.1 18.2 18.2 34.4 33.6 33.9 33.8 33.1 33.9 33.4 33.8 33.7 33.3 1092 1156 1254 1244 970 1194 1354 1233 1131 804 MEAN S.D. N 8.33 .455 10 15.0 .71 10 44.6 1.87 10 53.6 1.45 10 18.0 .38 10 33.7 .37 10 1143 158.3 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C90905 C90907 C90917 C90921 C90934 C90947 C90953 C90957 C90960 C90968 8.12 7.48 8.35 7.87 8.05 8.36 8.19 8.80 8.34 8.24 15.4 14.3 15.0 14.6 14.6 15.6 15.1 15.5 15.1 14.8 45.3 42.0 45.2 42.4 43.2 46.3 45.4 46.5 44.2 43.8 55.8 56.2 54.1 53.9 53.7 55.4 55.4 52.9 53.0 53.2 19.0 19.2 18.0 18.6 18.1 18.7 18.5 17.6 18.1 17.9 34.1 34.1 33.2 34.5 33.7 33.8 33.4 33.3 34.2 33.7 1148 1167 1148 1262 1159 1119 938 1171 1147 1152 MEAN S.D. N 8.18 .347 10 15.0 .43 10 44.4 1.57 10 54.4 1.23 10 18.4 .51 10 33.8 .42 10 1141 80.6 10 Covance 6329-212 3m T-6316.1 1464 ANIMAL NUMBER ------ Group: 1 C90716 C90731 C90738 C90745 C90746 C90748 C90760 C90766 C90768 C90780 MEAN S.D. N Group: 2 C90791 C90797 C90802 C90807 C90816 C90818 C90820 C90831 C90836 C90840 MEAN S.D. N Appendix 5 Covance 6329-212 3M T-6316.1 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 0 Dosage Unit: ppm 8.2 .8 6.6 .6 .2 .0 10 81 7 2 0 7.4 .9 5.7 .6 .2 .0 12 77 8 3 0 8.3 1.5 5.7 .9 .1 .0 18 69 11 2 0 8.2 1.0 6.3 .8 .1 .0 12 76 10 2 0 8.2 1.5 6.0 .5 .1 .0 18 74 6 2 0 6.8 1.1 4.8 .7 .1 .0 17 71 10 2 0 7.7 2.1 4.4 1.1 .1 .0 27 57 15 1 0 8.9 1.4 6.8 .7 .1 .0 15 76 7 1 0 5.1 1.1 3.4 .5 .1 .0 22 67 10 2 0 5.2 1.1 3.3 .7 .1 .0 21 64 13 2 0 7.4 1.2 5.3 .7 .1 .0 17 71 10 2 0 1.31 .38 1.27 .19 .04 .00 5.2 7.1 2.8 .6 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 3 Dosage Unit: ppm 9.5 1.0 7.8 .6 .1 .0 11 82 6 1 0 5.7 1.0 4.1 .5 .1 .0 18 71 8 2 0 12.7 1.3 10.5 .7 .1 .0 10 83 6 1 0 7.7 1.4 5.6 .5 .1 .0 18 73 6 2 0 12.5 2.4 8.6 1.0 .4 .0 20 69 8 3 0 17.7 2.4 13.8 1.3 .1 .0 14 78 7 1 0 9.6 1.5 6.7 1.2 .1 .0 16 70 13 2 0 8.3 1.9 5.4 .8 .2 .0 23 65 9 3 0 9.9 1.3 8.0 .5 .2 .0 13 81 5 2 0 10.7 1.0 8.7 .7 .3 .0 9 82 6 2 0 10.4 1.5 7.9 .8 .2 .0 15 75 7 2 0 3.31 .54 2.79 .29 .11 .00 4.6 6.6 2.3 .7 .0 10 10 10 10 10 10 10 10 10 10 10 1465 ANIMAL NUMBER ------ Group: 3 C90843 C90851 C90852 C90856 C90863 C90877 C90880 C90888 C90892 C90897 MEAN S.D. N Group: 4 C90905 C90907 C90917 C90921 C90934 C90947 C90953 C90957 C90960 C90968 MEAN S.D. N Appendix 5 Covance 6329-212 3M T-6316.1 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 30 Dosage Unit: ppm 12.6 2.1 9.1 1.2 .2 .0 16 72 9 2 0 10.6 .9 8.8 .7 .1 .0 8 83 7 1 0 6.3 .8 4.7 .6 .1 .0 13 74 10 2 0 10.8 1.1 9.0 .6 .1 .0 10 83 6 1 0 9.6 1.2 7.3 .7 .3 .0 13 77 8 3 0 7.3 1.6 5.4 .2 .2 .0 22 73 3 2 0 8.5 1.0 6.5 .7 .2 .0 12 77 8 2 0 8.4 1.7 6.2 .4 .1 .0 20 74 5 2 0 8.2 1.6 5.5 .8 .2 .0 20 68 10 2 0 9.7 1.4 7.1 1.0 .1 .0 15 73 10 2 0 9.2 1.3 7.0 .7 .2 .0 15 75 8 2 0 1.85 .41 1.59 .28 .07 .00 4.6 4.7 2.4 .6 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 100 Dosage Unit: ppm 11.3 1.5 9.1 .6 .2 .0 13 80 5 2 0 7.9 1.3 5.9 .6 .1 .0 17 75 7 1 0 5.8 .7 4.3 .7 .1 .0 13 74 11 2 0 8.1 1.3 6.2 .5 .1 .0 16 77 6 1 0 4.7 .6 3.5 .5 .1 .0 12 75 11 1 0 8.2 1.2 6.2 .7 .1 .0 14 76 9 1 0 12.2 1.9 8.8 1.3 .2 .0 16 72 11 1 0 9.0 1.6 6.6 .6 .2 .0 18 73 6 2 0 7.9 1.3 6.0 .5 .1 .0 17 76 6 1 0 12.9 1.3 10.8 .7 .2 .0 10 83 5 1 0 8.8 1.3 6.7 .7 .1 .0 15 76 8 1 0 2.64 .39 2.23 .24 .05 .00 2.6 3.3 2.5 .5 .0 10 10 10 10 10 10 10 10 10 10 10 1466 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90716 - - - - - C90731 - - - - - C90738 - - - - - C90745 - - - - - C90746 - - - - - C90748 - - - - - C90760 - - - - - C90766 - - - - - C90768 - - - - - C90780 - - - - - Group: 2 Dose Level: 3 Dosage Unit: ppm C90791 - - - - - C90797 - - - - - C90802 - - - - - C90807 - - - - - C90816 - - - - - C90818 - - - - - C90820 - - - - - C90831 - - - - - C90836 - - - - - C90840 - - - - - Covance 6329-212 3M T-6316.1 1467 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90843 - - - - - C90851 - - - - - C90852 - - - - - C90856 - - - - - C90863 - - - - - C90877 - - - - - C90880 - - - - - C90888 - - - - - C90892 - - - - - C90897 - - - - - Group: 4 Dose Level: 100 Dosage Unit: ppm C90905 - - - - - C90907 - - - - - C90917 - - - - - C90921 - - - - - C90934 - - - - - C90947 - - - - - C90953 - - - - - C90957 - - - - - C90960 - - - - - C90968 - - - - - Covance 6329-212 3M T-6316.1 1468 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91122 C91129 C91148 C91155 C91160 C91174 C91176 C91178 C91181 C91182 8.10 7.63 9.08 8.20 7.45 8.33 8.09 8.14 7.85 8.62 15.6 15.3 16.6 15.3 14.6 15.9 14.7 15.2 15.1 16.3 45.9 45.1 49.8 46.1 42.6 47.2 44.6 45.5 44.2 48.4 56.7 59.1 54.8 56.2 57.1 56.6 55.2 55.9 56.3 56.1 19.3 20.1 18.3 18.7 19.7 19.1 18.1 18.6 19.2 18.9 34.0 34.0 33.4 33.3 34.4 33.8 32.9 33.3 34.1 33.6 1223 1074 958 1034 960 1031 1203 987 1079 846 MEAN S.D. N 8.15 .469 10 15.5 .65 10 45.9 2.09 10 56.4 1.17 10 19.0 .61 10 33.7 .46 10 1040 113.7 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C91192 C91196 C91204 C91215 C91221 C91223 C91228 C91233 C91241 C91248 7.71 7.98 7.73 8.50 7.68 8.07 8.48 8.03 8.10 8.96 14.7 14.4 15.3 15.8 15.3 14.9 16.2 14.6 15.2 16.2 43.3 43.0 45.7 46.8 45.1 44.6 48.6 43.0 44.1 48.8 56.2 53.8 59.1 55.0 58.7 55.3 57.3 53.6 54.5 54.5 19.1 18.1 19.8 18.5 19.9 18.4 19.1 18.2 18.7 18.1 34.0 33.5 33.4 33.7 33.9 33.4 33.4 34.0 34.4 33.2 990 1105 865 1143 1086 1179 1139 1162 882 454 MEAN S.D. N 8.12 .411 10 15.3 .64 10 45.3 2.16 10 55.8 1.97 10 18.8 .67 10 33.7 .38 10 1000 222.8 10 Covance 6329-212 3M T-6316.1 1469 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91262 C91274 C91281 C91284 C91286 C91288 C91293 C91299 C91304 C91305 8.66 8.19 8.30 7.81 7.51 7.55 7.48 8.48 7.91 8.17 15.9 15.9 15.6 14.9 15.5 14.6 14.9 15.6 14.3 15.3 47.4 47.2 46.9 44.8 45.2 43.3 43.8 47.0 43.3 45.8 54.7 57.6 56.5 57.4 60.2 57.3 58.5 55.4 54.8 56.1 18.4 19.5 18.8 19.1 20.7 19.4 19.9 18.4 18.0 18.8 33.6 33.8 33.2 33.2 34.4 33.8 34.0 33.2 32.9 33.5 1313 913 1112 1065 949 1044 903 1147 994 1097 MEAN S.D. N 8.01 .419 10 15.2 .55 10 45.5 1.63 10 56.8 1.72 10 19.1 .80 10 33.6 .45 10 1054 124.0 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C91326 C91327 NR C91329 C91332 C91337 C91348 C91355 C91370 C91374 C91379 7.66 - 8.07 7.85 7.73 7.82 7.53 8.24 7.39 7.81 14.9 - 15.4 15.0 14.9 15.0 14.7 14.6 14.7 14.9 43.2 - 45.5 43.1 44.3 43.8 43.6 43.8 42.6 43.7 56.4 - 56.4 55.0 57.4 56.0 57.9 53.2 57.6 55.9 19.5 - 19.1 19.1 19.3 19.2 19.5 17.7 19.9 19.1 34.6 - 33.9 34.8 33.6 34.3 33.6 33.2 34.5 34.2 1035 - 1113 1017 1037 1016 994 968 1091 932 MEAN S.D. N 7.79 .258 9 14.9 .23 9 43.7 .82 9 56.2 1.46 9 19.2 .61 9 34.1 .54 9 1023 56.2 9 Covance 6329-212 3m T-6316.1 1470 ANIMAL NUMBER ------ Group: 1 C91122 C91129 C91148 C91155 C91160 C91174 C91176 C91178 C91181 C91182 MEAN S.D. N Group: 2 C91192 C91196 C91204 C91215 C91221 C91223 C91228 C91233 C91241 C91248 MEAN S.D. N Appendix 5 Covance 6329-212 3M T-6316.1 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 0 Dosage Unit: ppm 5.5 .8 4.2 .4 .1 .0 14 75 7 3 0 7.2 1.2 5.6 .3 .1 .0 17 78 4 1 0 8.8 .7 7.4 .5 .2 .0 8 84 6 2 0 3.9 .6 3.0 .2 .1 .0 15 77 6 2 0 4.7 .6 3.9 .2 .1 .0 12 83 3 1 0 6.6 .6 5.4 .5 .1 .0 10 82 7 1 0 7.7 .7 6.3 .6 .1 .0 9 82 7 2 0 5.9 1.1 4.2 .4 .1 .0 19 71 7 2 0 5.6 .9 4.2 .4 .1 .0 16 75 7 2 0 8.6 1.0 6.9 .5 .2 .0 12 80 6 2 0 6.4 .8 5.1 .4 .1 .0 13 79 6 2 0 1.63 .22 1.44 .13 .04 .00 3.6 4.2 1.4 .6 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 3 Dosage Unit: ppm 5.0 .9 3.7 .3 .1 .0 18 74 6 2 0 5.7 .4 4.6 .4 .2 .0 8 82 7 3 0 8.1 1.4 5.9 .7 .1 .0 17 73 8 1 0 6.7 .5 5.7 .3 .1 .0 8 86 5 1 0 9.4 1.4 7.6 .3 .1 .0 15 81 3 1 0 5.4 .3 4.5 .4 .1 .0 6 84 8 2 0 9.2 1.1 7.5 .5 .1 .0 12 82 5 1 0 5.7 .7 4.3 .5 .1 .0 12 76 9 3 0 8.5 .7 7.2 .4 .1 .0 9 85 5 1 0 4.9 .2 4.2 .4 .1 .0 5 85 9 1 0 6.9 .8 5.5 .4 .1 .0 11 81 6 2 0 1.77 .43 1.48 .12 .03 .00 4.5 4.8 2.0 .8 .0 10 10 10 10 10 10 10 10 10 10 10 1471 ANIMAL NUMBER ------ Group: 3 C91262 C91274 C91281 C91284 C91286 C91288 C91293 C91299 C91304 C91305 MEAN S.D. N Group: 4 C91326 C91327 NR C91329 C91332 C91337 C91348 C91355 C91370 C91374 C91379 MEAN S.D. N Appendix 5 Covance 6329-212 3M T-6316.1 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 30 Dosage Unit: ppm 5.8 .8 4.7 .2 .1 .0 13 80 4 2 0 22.5 1.2 20.2 .8 .4 .0 5 89 3 2 0 5.1 .6 4.1 .3 .1 .0 11 81 7 1 0 7.6 .7 6.3 .5 .1 .0 9 83 7 1 0 6.7 .5 5.8 .3 .1 .0 7 86 5 1 0 4.0 .6 3.0 .3 .1 .0 16 74 8 2 0 4.4 .8 3.3 .3 .1 .0 17 75 6 2 0 6.8 .7 5.6 .4 .1 .0 11 82 6 1 0 4.6 1.1 3.3 .1 .1 .0 24 70 3 2 0 7.8 .8 6.6 .3 .1 .0 10 85 4 2 0 7.5 .8 6.3 .4 .1 .0 12 80 5 2 0 5.43 .22 5.06 .19 .09 .00 5.5 5.9 1.8 .5 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 100 Dosage Unit: ppm 4.7 .5 3.6 .5 .1 .0 10 77 11 2 0 - - - - - - - - - - - 8.4 .5 7.3 .5 .1 .0 6 87 6 1 0 8.6 1.2 7.0 .2 .1 .0 14 82 2 1 0 6.1 .6 4.8 .4 .2 .0 10 79 7 3 0 8.1 .6 6.9 .4 .1 .0 8 85 5 2 0 4.2 .9 2.8 .5 .1 .0 22 65 11 2 0 6.1 .4 5.2 .4 .1 .0 7 85 6 1 0 5.5 .6 4.5 .3 .0 .0 12 81 6 1 0 4.0 .4 3.4 .2 .0 .0 11 83 4 1 0 6.2 .6 5.1 .4 .1 .0 11 80 6 2 0 1.80 .26 1.68 .12 .06 .00 4.8 6.6 3.0 .7 .0 9 9 9 9 9 9 9 9 9 9 9 1472 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91122 - - - - - C91129 - - - - - C91148 - - - - - C91155 - - - - - C91160 - - - - - C91174 - - - - - C91176 - - - - - C91178 - - - - - C91181 - - - - - C91182 - - - - - Group: 2 Dose Level: 3 Dosage Unit: ppm C91192 - - - - - C91196 - - - - - C91204 - - - - - C91215 - - - - - C91221 - - - - - C91223 - - - - - C91228 - - - - - C91233 - - - - - C91241 - - - - - C91248 - - - - - Covance 6329-212 3M T-6316.1 1473 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91262 - - - - - C91274 - - - - - C91281 - - - - - C91284 - - - - - C91286 - - - - - C91288 - - - - - C91293 - - - - - C91299 - - - - - C91304 - - - - - C91305 - - - - - Group: 4 Dose Level: 100 Dosage Unit: ppm C91326 - - - - - C91327 QS QS QS QS QS C91329 - - - - - C91332 - - - - - C91337 - - - - - C91348 - - - - - C91355 - - - - - C91370 - - - - - C91374 - - - - - C91379 - - - - - Covance 6329-212 3M T-6316.1 1474 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90721 C90735 C90736 C90738 C90739 C90747 C90751 C90752 C90755 C90766 7.65 8.29 8.49 8.30 8.47 7.52 8.77 8.94 8.30 8.56 13.8 15.4 14.7 15.0 15.0 14.3 15.1 15.2 14.6 14.6 41.8 45.6 44.1 44.7 44.7 42.2 45.7 45.7 43.2 44.5 54.6 55.0 51.9 53.9 52.8 56.0 52.1 51.1 52.0 52.0 18.0 18.6 17.3 18.0 17.7 19.0 17.2 17.0 17.6 17.1 33.1 33.8 33.4 33.4 33.6 34.0 32.9 33.1 33.7 32.8 1345 1213 1132 1314 1172 1131 1234 1414 1334 1407 MEAN S.D. N 8.33 .445 10 14.8 .47 10 44.2 1.41 10 53.1 1.63 10 17.8 .66 10 33.4 .40 10 1270 107.3 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C90795 C90799 C90802 C90803 C90805 C90806 C90824 C90825 C90839 C90840 8.88 7.52 9.11 8.34 9.16 8.48 8.13 7.96 8.19 6.17 16.1 13.9 15.3 14.6 16.1 14.5 15.3 14.6 15.1 11.0 47.8 41.8 46.7 44.9 47.3 43.3 45.4 43.8 45.4 33.4 53.9 55.6 51.3 53.8 51.6 51.1 55.9 55.0 55.4 54.1 18.1 18.4 16.8 17.5 17.5 17.0 18.8 18.3 18.5 17.9 33.7 33.2 32.7 32.5 34.0 33.4 33.6 33.4 33.4 33.1 1014 1018 1180 1023 1287 1148 1161 1135 1018 975 MEAN S.D. N 8.19 .879 10 14.6 1.46 10 44.0 4.15 10 53.8 1.83 10 17.9 .66 10 33.3 .45 10 1096 100.5 10 Covance 6329-212 3M T-6316.1 1475 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90847 C90855 C90858 C90859 C90864 C90865 C90871 C90872 C90882 C90886 8.19 8.47 9.08 8.32 8.59 8.16 7.80 7.87 8.04 8.20 14.5 14.4 15.6 14.6 15.0 15.5 14.0 15.0 15.3 15.1 43.1 43.0 45.6 43.3 44.9 44.9 42.2 43.5 45.1 44.4 52.6 50.8 50.2 52.0 52.2 55.0 54.2 55.3 56.1 54.1 17.7 17.0 17.2 17.6 17.5 19.0 18.0 19.1 19.0 18.4 33.6 33.4 34.2 33.8 33.5 34.4 33.2 34.5 33.8 34.1 965 1322 1269 1277 1260 1011 1207 1057 1203 1326 MEAN S.D. N 8.27 .374 10 14.9 .51 10 44.0 1.12 10 53.2 1.98 10 18.0 .78 10 33.8 .44 10 1190 131.4 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C90916 C90931 C90937 C90940 C90944 C90945 C90954 C90956 C90957 C90963 8.20 8.10 8.04 8.64 7.93 8.55 7.91 8.67 8.69 7.52 14.5 14.3 13.2 14.8 14.4 15.3 14.6 14.4 15.2 14.1 43.2 41.6 39.0 43.6 42.1 45.8 43.2 42.8 46.4 41.7 52.7 51.4 48.5 50.5 53.1 53.6 54.5 49.4 53.4 55.4 17.7 17.6 16.4 17.1 18.2 17.8 18.5 16.6 17.5 18.8 33.6 34.3 33.9 33.9 34.2 33.3 33.9 33.5 32.8 33.9 1142 1169 1185 1243 1327 1214 1202 1246 1193 1142 MEAN S.D. N 8.22 .398 10 14.5 .59 10 42.9 2.12 10 52.2 2.24 10 17.6 .77 10 33.7 .44 10 1206 55.6 10 Covance 6329-212 3M T-6316.1 1476 ANIMAL NUMBER ------ Group: 1 C90721 C90735 C90736 C90738 C90739 C90747 C90751 C90752 C90755 C90766 MEAN S.D. N Group: 2 C90795 C90799 C90802 C90803 C90805 C90806 C90824 C90825 C90839 C90840 MEAN S.D. N Appendix 5 Covance 6329-212 3M T-6316.1 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 0 Dosage Unit: ppm 7.9 2.1 4.8 .9 .1 .0 26 61 11 2 0 7.3 1.7 4.8 .6 .2 .0 23 66 8 2 0 4.7 1.0 3.1 .4 .2 .0 22 65 9 3 0 7.2 1.3 4.9 .9 .1 .0 18 68 12 2 0 7.6 1.4 5.5 .6 .2 .0 18 72 8 2 0 8.8 1.4 6.5 .7 .1 .0 16 74 8 2 0 6.6 1.0 4.6 .8 .2 .0 15 69 12 2 0 11.6 1.4 8.9 1.0 .3 .0 12 77 8 2 0 8.6 1.3 6.0 1.0 .2 .0 16 70 12 3 0 8.4 1.8 5.6 .8 .2 .0 21 66 10 2 0 7.9 1.4 5.5 .8 .2 .0 19 69 10 2 0 1.77 .34 1.52 .19 .06 .00 4.2 4.7 1.8 .4 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 3 Dosage Unit: ppm 6.5 1.3 4.1 1.0 .2 .0 20 62 15 3 0 7.7 1.2 6.0 .5 .1 .0 15 77 6 1 0 8.2 1.4 6.2 .4 .1 .0 17 76 5 1 0 7.6 1.5 5.0 .9 .2 .0 20 66 12 3 0 8.4 1.9 5.9 .5 .1 .0 22 70 6 2 0 6.4 .8 5.0 .4 .1 .0 13 79 6 2 0 8.4 1.0 6.3 .8 .3 .0 12 75 10 3 0 8.0 1.3 5.6 .9 .1 .0 16 70 12 2 1 6.0 .8 4.2 .8 .1 .0 13 71 14 2 0 11.8 3.0 8.0 .5 .3 .0 26 68 4 2 0 7.9 1.4 5.6 .7 .2 .0 17 71 9 2 0 1.62 .65 1.14 .23 .08 .00 4.5 5.3 4.1 .7 .3 10 10 10 10 10 10 10 10 10 10 10 1477 ANIMAL NUMBER ------ Group: 3 C90847 C90855 C90858 C90859 C90864 C90865 C90871 C90872 C90882 C90886 MEAN S.D. N Group: 4 C90916 C90931 C90937 C90940 C90944 C90945 C90954 C90956 C90957 C90963 MEAN S.D. N Appendix 5 Covance 6329-212 3M T-6316.1 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 30 Dosage Unit: ppm 10.8 2.3 7.4 .8 .2 .0 22 69 7 1 0 10.3 3.8 5.6 .7 .2 .0 37 54 7 2 0 8.7 1.9 5.7 1.0 .1 .0 22 66 11 1 0 9.6 2.9 5.6 1.0 .1 .0 30 58 11 2 0 11.0 2.0 7.8 .9 .3 .0 18 71 8 3 0 9.1 1.1 6.9 .9 .1 .0 12 76 10 1 0 7.8 1.4 5.6 .6 .1 .0 18 72 8 1 0 5.9 1.0 4.4 .3 .1 .0 16 76 6 2 0 5.4 .7 4.1 .4 .2 .0 12 77 8 3 0 9.1 2.8 5.0 1.1 .1 .0 30 56 13 1 0 8.8 2.0 5.8 .8 .2 .0 22 68 9 2 0 1.91 .98 1.22 .27 .07 .00 8.3 8.7 2.2 .8 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 100 Dosage Unit: ppm 8.1 2.0 5.4 .5 .1 .0 25 67 6 1 0 5.6 1.0 3.9 .6 .1 .0 17 70 11 2 1 7.7 2.1 4.7 .7 .1 .0 28 61 9 2 0 9.3 1.7 6.9 .6 .1 .0 18 74 7 1 0 9.9 1.6 7.5 .6 .1 .0 16 76 6 1 0 5.7 1.2 3.5 .8 .1 .0 22 62 14 1 0 8.6 1.1 6.5 .7 .2 .0 13 76 8 2 0 8.8 2.4 5.5 .8 .1 .0 27 62 9 2 0 8.4 1.8 5.6 .8 .2 .0 21 66 10 3 0 16.6 8.9 6.1 1.5 .1 .0 54 36 9 1 0 8.9 2.4 5.6 .8 .1 .0 24 65 9 2 0 3.06 2.34 1.27 .28 .04 .00 11.6 11.7 2.4 .7 .3 10 10 10 10 10 10 10 10 10 10 10 1478 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90721 - - - - - C90735 - - - - - C90736 - - - - - C90738 - - - - - C90739 - - - - - C90747 - - - - - C90751 - - - - - C90752 - - - - - C90755 - - - - - C90766 - - - - - Group: 2 Dose Level: 3 Dosage Unit: ppm C90795 - - - - - C90799 - - - - - C90802 - - - - - C90803 - - - - - C90805 - - - - - C90806 - - - - - C90824 - - - - - C90825 - - - - - C90839 - - - - - C90840 - - - - - Covance 6329-212 3M T-6316.1 1479 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90847 - - - - - C90855 - - - - - C90858 - - - - - C90859 - - - - - C90864 - - - - - C90865 - - - - - C90871 - - - - - C90872 - - - - - C90882 - - - - - C90886 - - - - - Group: 4 Dose Level: 100 Dosage Unit: ppm C90916 - - - - - C90931 - - - - - C90937 - - - - - C90940 - - - - - C90944 - - - - - C90945 - - - - - C90954 - - - - - C90956 - - - - - C90957 - - - - - C90963 - - - - - Covance 6329-212 3M T-6316.1 1480 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91127 C91128 C91133 C91137 C91144 C91153 C91154 C91158 C91161 C91183 7.95 7.84 8.20 8.24 8.46 8.00 7.91 7.42 7.56 7.74 15.5 15.4 15.6 15.1 15.3 14.9 15.4 14.7 15.5 15.4 45.5 45.3 46.7 44.7 46.2 44.1 45.3 43.0 45.3 44.8 57.3 57.7 57.0 54.3 54.5 55.1 57.2 57.9 59.9 58.0 19.5 19.6 19.0 18.4 18.1 18.6 19.5 19.8 20.5 20.0 34.0 34.0 33.4 33.8 33.2 33.8 34.0 34.3 34.2 34.4 1148 926 1078 994 1164 1098 1226 1000 981 1140 MEAN S.D. N 7.93 .316 10 15.3 .29 10 45.1 1.04 10 56.9 1.76 10 19.3 .76 10 33.9 .38 10 1076 96.6 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C91196 C91207 C91210 C91211 C91214 C91221 C91224 C91235 C91243 C91244 7.55 7.80 7.24 7.59 7.27 7.84 7.87 7.39 7.27 7.60 14.5 14.3 14.6 14.8 14.4 15.5 15.3 14.8 15.0 13.8 42.2 42.5 43.2 44.1 42.4 45.1 45.2 42.8 43.5 41.5 55.8 54.5 59.7 58.1 58.4 57.6 57.5 58.0 59.8 54.6 19.2 18.3 20.2 19.5 19.9 19.8 19.4 20.1 20.7 18.1 34.5 33.6 33.8 33.6 34.0 34.4 33.8 34.6 34.6 33.2 1081 1029 858 1286 841 1135 1058 1024 891 665 MEAN S.D. N 7.54 .243 10 14.7 .50 10 43.2 1.23 10 57.4 1.88 10 19.5 .82 10 34.0 .49 10 987 176.1 10 Covance 6329-212 3M T-6316.1 1481 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91252 C91254 C91264 C91267 C91283 C91286 C91292 C91295 C91301 C91303 7.82 7.71 7.71 7.16 7.94 7.25 7.58 8.37 8.32 7.96 14.4 14.5 15.3 14.1 15.0 15.1 14.5 15.0 15.5 15.2 42.4 42.5 43.8 41.7 44.5 44.0 43.3 45.4 45.6 45.4 54.2 55.2 56.9 58.2 56.0 60.6 57.1 54.2 54.8 57.0 18.4 18.8 19.8 19.7 18.9 20.8 19.2 18.0 18.6 19.1 33.9 34.1 34.9 33.8 33.8 34.3 33.5 33.1 33.9 33.5 1010 1119 890 990 853 882 880 961 990 1109 MEAN S.D. N 7.78 .396 10 14.9 .46 10 43.9 1.38 10 56.4 1.99 10 19.1 .80 10 33.9 .49 10 968 94.2 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C91312 C91323 C91328 C91339 C91340 C91351 C91354 C91357 C91369 C91371 7.36 7.14 7.74 7.37 7.16 7.22 7.45 6.92 6.80 7.33 14.1 13.5 15.5 14.3 14.3 14.3 14.0 13.9 13.2 13.8 40.6 39.7 45.2 41.3 41.6 42.0 41.5 39.8 39.3 38.9 55.1 55.6 58.4 56.1 58.0 58.2 55.7 57.4 57.9 53.0 19.1 18.8 20.1 19.4 19.9 19.8 18.9 20.0 19.5 18.8 34.7 33.9 34.4 34.6 34.4 34.1 33.8 34.9 33.7 35.5 1181 793 944 996 949 1046 1008 1043 922 1076 MEAN S.D. N 7.25 .268 10 14.1 .61 10 41.0 1.82 10 56.5 1.74 10 19.4 .51 10 34.4 .56 10 996 103.9 10 Covance 6329-212 3M T-6316.1 1482 ANIMAL NUMBER ------ Group: 1 C91127 C91128 C91133 C91137 C91144 C91153 C91154 C91158 C91161 C91183 MEAN S.D. N Group: 2 C91196 C91207 C91210 C91211 C91214 C91221 C91224 C91235 C91243 C91244 MEAN S.D. N Appendix 5 Covance 6329-212 3M T-6316.1 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 0 Dosage Unit: ppm 6.9 .6 5.9 .3 .1 .0 9 85 5 1 0 4.5 .4 3.6 .5 .1 .0 9 79 10 2 0 4.3 .8 2.3 1.0 .1 .1 19 54 23 2 2 3.9 .7 2.6 .4 .1 .0 19 67 11 3 0 3.4 .8 1.9 .6 .1 .0 23 57 17 2 1 6.2 .6 5.0 .5 .1 .0 9 81 8 2 0 5.0 .4 3.6 .8 .1 .0 8 73 16 2 1 3.7 .6 2.4 .6 .1 .0 15 65 16 3 0 6.3 .9 4.7 .6 .1 .0 14 74 10 2 0 6.4 .8 5.0 .4 .1 .0 13 78 7 2 0 5.1 .7 3.7 .6 .1 .0 14 71 12 2 0 1.29 .17 1.39 .21 .00 .03 5.2 10.3 5.5 .6 .7 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 3 Dosage Unit: ppm 3.6 .4 2.8 .3 .1 .0 11 77 8 4 0 5.2 .9 3.6 .5 .1 .0 18 70 9 3 0 5.0 .5 3.8 .6 .1 .0 10 76 12 1 0 2.9 .5 2.0 .3 .1 .0 18 68 11 3 0 9.6 1.2 7.6 .6 .1 .0 13 79 6 1 0 6.5 .9 5.1 .2 .1 .0 15 79 4 2 0 5.4 .6 4.1 .6 .2 .0 11 76 10 3 0 3.9 .6 2.8 .4 .1 .0 16 73 9 2 0 4.9 .7 3.4 .7 .1 .0 14 69 15 2 0 5.1 .7 3.7 .6 .1 .0 13 73 12 2 1 5.2 .7 3.9 .5 .1 .0 14 74 10 2 0 1.85 .24 1.55 .17 .03 .00 2.8 4.0 3.2 .9 .3 10 10 10 10 10 10 10 10 10 10 10 1483 ANIMAL NUMBER ------ Group: 3 C91252 C91254 C91264 C91267 C91283 C91286 C91292 C91295 C91301 C91303 MEAN S.D. N Group: 4 C91312 C91323 C91328 C91339 C91340 C91351 C91354 C91357 C91369 C91371 MEAN S.D. N Appendix 5 Covance 6329-212 3M T-6316.1 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 30 Dosage Unit: ppm 4.0 .8 2.7 .4 .1 .0 20 68 9 3 0 6.6 1.3 4.2 .9 .1 .0 20 64 14 2 1 5.6 .8 4.0 .6 .1 .0 15 71 11 2 0 3.8 .8 2.6 .3 .2 .0 20 68 7 4 0 3.7 .8 2.1 .7 .1 .0 21 56 20 2 0 3.7 .4 3.0 .2 .1 .0 10 81 7 2 0 5.7 .8 4.0 .7 .1 .0 15 71 13 2 0 4.5 .8 3.1 .4 .1 .0 18 69 9 3 1 3.9 1.0 2.4 .5 .1 .0 25 61 12 2 0 6.6 .8 5.2 .4 .1 .0 12 79 7 2 0 4.8 .8 3.3 .5 .1 .0 18 69 11 2 0 1.20 .22 .98 .21 .03 .00 4.6 7.5 4.1 .7 .4 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 100 Dosage Unit: ppm 5.3 1.3 3.4 .4 .1 .0 25 64 8 2 1 4.1 .9 2.8 .4 .1 .0 21 67 9 3 0 4.6 1.2 2.9 .4 .1 .0 26 64 8 3 0 5.7 .7 4.4 .4 .1 .0 13 77 8 2 0 4.4 .5 3.5 .3 .1 .0 12 79 6 2 0 3.9 .6 2.8 .4 .1 .0 14 72 11 2 0 6.0 .6 4.9 .3 .1 .0 11 82 5 2 0 4.4 .8 3.0 .5 .1 .0 18 68 12 2 1 5.2 .6 4.1 .3 .1 .0 12 79 6 2 0 5.0 1.1 3.4 .3 .2 .0 22 68 7 3 0 4.9 .8 3.5 .4 .1 .0 17 72 8 2 0 .69 .28 .73 .07 .03 .00 5.7 6.7 2.2 .5 .4 10 10 10 10 10 10 10 10 10 10 10 1484 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91127 - - - - - C91128 - - - - - C91133 - - - - - C91137 - - - - - C91144 - - - - - C91153 - - - - - C91154 - - - - - C91158 - - - - - C91161 - - - - - C91183 - - - - - Group: 2 Dose Level: 3 Dosage Unit: ppm C91196 - - - - - C91207 - - - - - C91210 - - - - - C91211 - - - - - C91214 - - - - - C91221 - - - - - C91224 - - - - - C91235 - - - - - C91243 - - - - - C91244 - - - - - Covance 6329-212 3M T-6316.1 1485 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91252 - - - - - C91254 - - - - - C91264 - - - - - C91267 - - - - - C91283 - - - - - C91286 - - - - - C91292 - - - - - C91295 - - - - - C91301 - - - - - C91303 - - - - - Group: 4 Dose Level: 100 Dosage Unit: ppm C91312 - - - - - C91323 - - - - - C91328 - - - - - C91339 - - - - - C91340 - - - - - C91351 - - - - - C91354 - - - - - C91357 - - - - - C91369 - - - - - C91371 - - - - - Covance 6329-212 3M T-6316.1 1486 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90712 C90723 C90727 C90730 C90755 C90758 C90759 C90764 C90766 C90774 7.45 8.79 8.47 7.45 8.04 7.99 9.02 8.42 8.86 5.62 13.8 15.5 15.0 14.6 14.1 14.9 15.4 15.4 15.6 10.5 41.6 46.6 44.6 43.2 41.8 44.4 46.0 46.7 46.4 31.7 55.8 53.0 52.6 58.0 51.9 55.5 51.0 55.5 52.4 56.5 18.6 17.6 17.7 19.7 17.6 18.7 17.1 18.3 17.6 18.7 33.2 33.2 33.5 33.9 33.8 33.7 33.4 33.0 33.6 33.2 1392 1226 1667 1006 1283 1180 1369 1074 1338 848 MEAN S.D. N 8.01 1.004 10 14.5 1.52 10 43.3 4.50 10 54.2 2.32 10 18.2 .78 10 33.4 .30 10 1238 229.3 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C90781 C90792 C90793 C90798 C90799 C90800 C90812 C90822 C90829 C90832 8.01 8.87 8.99 9.10 7.61 8.73 8.48 8.33 7.94 8.14 14.8 16.3 16.1 15.6 14.3 15.6 15.8 14.8 14.6 14.2 43.8 48.6 48.3 46.3 41.8 46.6 48.4 45.2 42.6 41.3 54.7 54.8 53.7 50.9 54.9 53.3 57.1 54.2 53.7 50.8 18.5 18.4 17.9 17.1 18.7 17.9 18.6 17.8 18.4 17.5 33.7 33.6 33.3 33.6 34.2 33.6 32.6 32.7 34.3 34.4 1105 1247 1505 1286 942 1031 1264 1278 1046 1400 MEAN S.D. N 8.42 .498 10 15.2 .76 10 45.3 2.79 10 53.8 1.88 10 18.1 .52 10 33.6 .61 10 1210 176.3 10 Covance 6329-212 3M T-6316.1 1487 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90841 C90850 C90854 C90855 C90856 C90859 C90873 C90879 C90881 C90896 8.65 8.74 7.03 9.31 8.48 8.25 8.52 8.09 8.60 8.26 14.5 15.0 11.9 16.0 15.2 14.8 15.0 15.0 14.5 15.8 44.2 44.7 35.3 47.3 45.1 44.5 44.1 44.4 43.1 45.2 51.1 51.1 50.2 50.8 53.2 53.9 51.7 54.8 50.1 54.7 16.8 17.2 16.9 17.2 18.0 18.0 17.6 18.5 16.8 19.2 32.9 33.7 33.7 33.8 33.8 33.3 34.0 33.8 33.6 35.1 1161 1201 1056 1328 1263 1190 1370 1182 1104 1133 MEAN S.D. N 8.39 .585 10 14.8 1.12 10 43.8 3.17 10 52.2 1.82 10 17.6 .80 10 33.8 .56 10 1199 97.5 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C90908 C90914 C90923 C90926 C90927 C90934 C90936 C90944 C90946 C90956 8.58 7.89 8.69 7.61 8.68 8.46 8.27 8.30 8.96 9.08 15.0 14.5 14.7 13.1 15.1 14.3 14.4 14.8 15.5 15.3 44.0 42.1 43.5 39.8 44.8 43.2 41.9 43.2 45.2 45.5 51.3 53.4 50.0 52.3 51.6 51.1 50.7 52.0 50.5 50.1 17.5 18.3 17.0 17.2 17.3 16.9 17.5 17.9 17.3 16.9 34.1 34.3 33.9 32.9 33.6 33.0 34.4 34.4 34.3 33.8 1015 1004 1367 831 1000 1173 1111 1450 1224 1118 MEAN S.D. N 8.45 .454 10 14.7 .68 10 43.3 1.73 10 51.3 1.06 10 17.4 .45 10 33.9 .55 10 1129 184.1 10 Covance 6329-212 3M T-6316.1 1488 ANIMAL NUMBER ------ Group: 1 C90712 C90723 C90727 C90730 C90755 C90758 C90759 C90764 C90766 C90774 MEAN S.D. N Group: 2 C90781 C90792 C90793 C90798 C90799 C90800 C90812 C90822 C90829 C90832 MEAN S.D. N Appendix 5 Covance 6329-212 3M T-6316.1 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 0 Dosage Unit: ppm 7.1 2.6 3.5 .8 .1 .0 37 50 11 1 0 6.7 1.4 3.8 1.3 .2 .0 21 57 19 3 1 11.1 3.0 7.0 .8 .3 .0 27 63 8 2 0 6.9 1.9 3.9 .9 .2 .0 28 56 14 2 0 8.7 2.1 5.1 1.1 .3 .0 25 59 13 3 0 12.5 4.5 6.0 1.7 .2 .0 36 48 14 2 0 8.9 2.1 5.8 .8 .2 .0 24 65 9 2 0 8.2 2.5 4.7 .8 .1 .0 31 57 10 2 0 9.3 2.5 5.8 .9 .1 .0 27 62 10 1 0 5.4 1.6 3.2 .4 .1 .0 30 59 8 2 0 8.5 2.4 4.9 1.0 .2 .0 29 58 12 2 0 2.14 .88 1.26 .35 .08 .00 5.1 5.4 3.4 .7 .3 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 3 Dosage Unit: ppm 8.2 2.4 5.0 .6 .1 .0 30 61 8 1 0 8.4 1.8 5.4 .8 .2 .0 22 65 10 2 0 7.7 1.7 5.1 .7 .1 .0 22 66 9 2 0 9.1 3.6 4.5 .9 .2 .0 39 49 10 2 0 6.9 1.8 3.9 1.0 .1 .0 27 56 15 1 0 5.4 1.1 3.7 .5 .1 .0 21 68 9 1 0 8.1 1.3 5.8 .7 .2 .0 16 72 8 3 0 7.5 1.5 4.6 1.2 .2 .0 20 61 16 2 0 8.9 1.6 5.7 1.4 .1 .1 18 64 16 1 1 8.8 2.5 5.0 1.1 .1 .0 29 57 12 1 0 7.9 1.9 4.9 .9 .1 .0 24 62 11 2 0 1.11 .73 .70 .28 .05 .03 6.9 6.6 3.2 .7 .3 10 10 10 10 10 10 10 10 10 10 10 1489 ANIMAL NUMBER ------ Group: 3 C90841 C90850 C90854 C90855 C90856 C90859 C90873 C90879 C90881 C90896 MEAN S.D. N Group: 4 C90908 C90914 C90923 C90926 C90927 C90934 C90936 C90944 C90946 C90956 MEAN S.D. N Appendix 5 Covance 6329-212 3M T-6316.1 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 30 Dosage Unit: ppm 9.7 3.4 4.9 1.3 .2 .0 34 50 13 2 0 9.7 3.1 6.4 .1 .1 .0 32 66 1 2 0 8.5 2.8 5.0 .5 .1 .0 33 60 6 1 0 7.7 1.3 5.8 .5 .1 .0 17 75 7 1 0 8.8 1.5 6.1 1.0 .2 .0 17 69 11 2 0 8.2 2.8 4.3 1.0 .1 .0 34 53 12 2 0 9.8 1.4 7.2 1.0 .2 .0 15 73 10 2 0 9.8 2.9 5.8 .9 .3 .0 29 59 9 3 0 8.8 3.2 5.5 .0 .1 .0 36 63 0 1 0 7.4 2.6 3.8 .9 .1 .0 34 52 12 1 0 8.8 2.5 5.5 .7 .2 .0 28 62 8 2 0 .90 .79 1.01 .43 .07 .00 8.3 8.8 4.6 .7 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 100 Dosage Unit: ppm 6.9 1.5 4.1 1.1 .2 .0 22 60 16 2 0 6.2 1.6 3.9 .5 .1 .0 26 63 9 2 0 8.3 2.4 4.6 1.1 .2 .0 29 55 13 2 0 7.8 1.0 6.0 .7 .1 .0 13 77 8 1 0 7.9 1.5 5.3 .9 .1 .0 19 67 12 1 0 3.7 1.0 1.8 .7 .1 .0 29 50 18 2 0 9.5 2.3 6.0 .9 .2 .0 24 64 9 2 0 10.6 2.1 7.6 .8 .2 .0 20 72 7 2 0 8.5 2.3 5.2 .7 .2 .0 27 62 9 3 0 5.3 2.0 2.4 .7 .1 .0 39 46 13 2 1 7.5 1.8 4.7 .8 .2 .0 25 62 11 2 0 2.02 .53 1.74 .19 .05 .00 7.1 9.5 3.6 .6 .3 10 10 10 10 10 10 10 10 10 10 10 1490 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90712 - - - - - C90723 - - - - - C90727 - - - - - C90730 - - - - - C90755 - - - - - C90758 - - - - - C90759 - - - - - C90764 - - - - - C90766 - - - - - C90774 1 - - - - Group: 2 Dose Level: 3 Dosage Unit: ppm C90781 - - - - - C90792 - - - - - C90793 - - - - - C90798 - - - - - C90799 - - - - - C90800 - - - - - C90812 - - - - - C90822 - - - - - C90829 - - - - - C90832 - - - - - Covance 6329-212 3M T-6316.1 1491 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90841 - - - - - C90850 - - - - - C90854 - - - - - C90855 - - - - - C90856 - - - - - C90859 - - - - - C90873 - - - - - C90879 - - - - - C90881 - - - - - C90896 - - - - - Group: 4 Dose Level: 100 Dosage Unit: ppm C90908 - - - - - C90914 - - - - - C90923 - - - - - C90926 - - - - - C90927 - - - - - C90934 - - - - - C90936 - - - - - C90944 - - - - - C90946 - - - - - C90956 - - - - - Covance 6329-212 3M T-6316.1 1492 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91123 C91125 C91131 C91132 C91153 C91156 C91158 C91178 C91180 C91189 7.38 7.82 8.47 7.48 7.97 4.19 7.81 7.60 5.24 8.30 14.7 14.4 15.3 15.0 15.1 10.3 15.8 14.7 11.1 16.1 43.0 42.8 45.5 43.2 44.6 28.7 46.1 42.9 31.8 46.7 58.2 54.7 53.8 57.8 56.0 68.6 59.0 56.5 60.7 56.3 20.0 18.5 18.1 20.1 18.9 24.5 20.2 19.3 21.1 19.4 34.3 33.7 33.7 34.7 33.8 35.8 34.2 34.2 34.8 34.5 851 870 1215 980 966 948 908 958 1113 1168 MEAN S.D. N 7.23 1.388 10 14.2 1.95 10 41.5 6.15 10 58.2 4.19 10 20.0 1.81 10 34.4 .64 10 998 125.1 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C91194 C91197 C91199 C91200 C91206 C91227 C91229 C91231 C91244 C91250 7.17 7.31 8.20 8.04 7.28 7.37 6.76 7.81 7.90 6.32 13.3 14.6 15.1 15.6 14.2 14.4 14.1 15.2 14.2 12.0 39.7 42.8 45.2 46.8 42.8 42.2 40.6 44.5 43.1 36.3 55.4 58.5 55.2 58.2 58.8 57.2 60.1 56.9 54.5 57.5 18.5 20.0 18.5 19.4 19.6 19.5 20.9 19.5 17.9 19.1 33.5 34.1 33.5 33.3 33.3 34.1 34.7 34.2 32.9 33.2 1103 819 931 994 890 1012 1029 881 669 1019 MEAN S.D. N 7.42 .589 10 14.3 1.03 10 42.4 2.99 10 57.2 1.78 10 19.3 .85 10 33.7 .56 10 935 125.9 10 Covance 6329-212 3M T-6316.1 1493 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91255 C91258 C91264 C91269 C91270 C91274 C91289 C91294 C91297 C91305 7.97 8.01 7.52 7.49 4.77 7.23 8.13 7.91 7.79 7.60 15.3 15.7 15.3 13.9 9.8 14.7 16.9 14.5 14.9 14.8 44.6 46.2 43.9 41.0 28.6 42.1 50.1 43.4 44.5 43.8 56.0 57.7 58.4 54.7 59.9 58.2 61.6 54.9 57.1 57.7 19.2 19.6 20.4 18.5 20.5 20.3 20.7 18.4 19.1 19.5 34.4 34.0 34.9 33.8 34.2 34.8 33.7 33.5 33.5 33.7 937 1198 775 924 948 692 922 1007 875 897 MEAN S.D. N 7.44 .979 10 14.6 1.86 10 42.8 5.57 10 57.6 2.13 10 19.6 .83 10 34.0 .51 10 918 133.9 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C91314 C91319 C91326 C91334 C91346 C91356 C91359 C91363 C91372 C91373 6.14 13.0 37.6 61.2 21.1 34.5 773 7.40 14.5 41.9 56.6 19.7 34.7 868 7.56 14.8 42.6 56.3 19.6 34.7 765 8.03 15.1 44.0 54.8 18.8 34.3 977 6.98 13.6 40.0 57.4 19.5 34.0 903 4.55 9.6 27.3 59.9 21.0 35.1 935 6.35 12.6 37.2 58.5 19.8 33.8 936 7.46 14.0 40.1 53.8 18.7 34.8 974 7.50 14.6 42.2 56.3 19.4 34.5 979 7.23 14.3 40.7 56.3 19.7 35.1 962 MEAN S.D. N 6.92 1.008 10 13.6 1.62 10 39.4 4.74 10 57.1 2.24 10 19.7 .79 10 34.6 .43 10 907 80.9 10 Covance 6329-212 3M T-6316.1 1494 ANIMAL NUMBER ------ Group: 1 C91123 C91125 C91131 C91132 C91153 C91156 C91158 C91178 C91180 C91189 MEAN S.D. N Group: 2 C91194 C91197 C91199 C91200 C91206 C91227 C91229 C91231 C91244 C91250 MEAN S.D. N Appendix 5 Covance 6329-212 3M T-6316.1 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 0 Dosage Unit: ppm 4.0 1.0 2.4 .4 .1 .0 26 60 11 3 0 5.0 .9 3.2 .7 .1 .0 19 64 15 2 1 3.4 .5 2.3 .5 .1 .0 16 67 14 2 1 5.0 .9 3.4 .6 .1 .0 19 68 12 2 0 4.2 .7 2.8 .5 .1 .0 18 68 12 2 0 4.9 1.2 3.0 .6 .1 .0 24 61 13 2 0 4.9 1.7 2.4 .6 .1 .0 35 50 13 2 0 4.0 1.2 2.2 .5 .1 .0 30 54 12 3 0 5.2 1.7 2.6 .6 .2 .0 34 51 12 3 0 4.3 1.2 2.5 .5 .1 .0 27 58 12 2 0 4.5 1.1 2.7 .6 .1 .0 25 60 13 2 0 .59 .39 .40 .08 .03 .00 6.8 6.8 1.2 .5 .4 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 3 Dosage Unit: ppm 5.3 1.2 3.4 .6 .1 .0 23 65 10 2 0 5.2 1.8 2.6 .6 .2 .0 34 50 11 4 0 4.2 .6 2.9 .6 .1 .0 14 70 14 1 0 3.2 .9 1.8 .4 .1 .0 28 57 13 2 0 6.4 1.4 4.2 .7 .1 .0 22 65 11 2 0 3.8 .6 2.8 .4 .1 .0 15 72 10 3 0 3.4 .8 2.1 .4 .1 .0 24 61 13 2 0 3.2 .7 2.0 .4 .1 .0 21 64 12 3 0 9.5 5.9 2.0 1.5 .1 .1 62 21 16 1 1 4.0 .8 2.7 .4 .1 .0 21 67 11 2 0 4.8 1.5 2.6 .6 .1 .0 26 59 12 2 0 1.95 1.60 .74 .34 .03 .03 13.8 14.8 1.9 .9 .3 10 10 10 10 10 10 10 10 10 10 10 1495 ANIMAL NUMBER ------ Group: 3 C91255 C91258 C91264 C91269 C91270 a C91274 C91289 C91294 C91297 C91305 MEAN S.D. N Group: 4 C91314 C91319 C91326 C91334 C91346 C91356 C91359 C91363 C91372 C91373 MEAN S.D. N Appendix 5 Covance 6329-212 3M T-6316.1 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 30 Dosage Unit: ppm 7.0 1.5 4.7 .7 .1 .0 22 67 9 2 0 6.0 2.3 3.0 .6 .1 .0 38 49 11 2 0 4.0 .8 2.7 .5 .1 .0 19 67 12 3 0 3.9 1.0 2.4 .3 .1 .0 25 63 9 3 0 6.2 1.7 4.3 .1 .1 .0 28 69 2 1 0 7.6 1.2 5.8 .5 .1 .0 16 76 6 2 0 8.1 1.1 6.2 .7 .1 .0 13 76 9 1 0 4.0 .6 2.7 .4 .3 .0 16 66 11 6 0 4.1 1.0 2.3 .7 .1 .0 24 57 16 3 0 5.9 1.0 4.3 .4 .2 .0 17 73 6 3 0 5.7 1.2 3.8 .5 .1 .0 22 66 9 3 0 1.60 .49 1.43 .20 .07 .00 7.4 8.4 3.8 1.4 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 100 Dosage Unit: ppm 5.6 1.7 2.8 1.0 .1 .0 30 50 18 2 0 6.1 1.6 4.0 .3 .2 .0 26 66 4 4 0 4.1 1.2 2.2 .7 .0 .0 30 53 16 1 0 6.6 1.4 4.2 .8 .2 .0 21 65 12 3 0 5.2 1.6 2.8 .7 .1 .0 31 54 13 2 0 6.2 2.0 3.5 .5 .2 .0 32 56 8 3 1 6.0 1.2 3.9 .7 .1 .0 21 65 11 2 0 6.5 1.8 3.8 .7 .1 .0 27 60 11 2 0 6.3 2.2 3.4 .5 .2 .0 34 54 8 3 0 5.7 1.4 3.3 .7 .3 .0 24 58 12 5 0 5.8 1.6 3.4 .7 .2 .0 28 58 11 3 0 .74 .33 .63 .19 .08 .00 4.6 5.7 4.0 1.2 .3 10 10 10 10 10 10 10 10 10 10 10 a Differential was read manually. 1496 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91123 - - - - - C91125 - - - - - C91131 - - - - - C91132 - - - - - C91153 - - - - - C91156 - 1 - - - C91158 - - - - - C91178 - - - - - C91180 1 - 1 - - C91189 - - - - - Group: 2 Dose Level: 3 Dosage Unit: ppm C91194 - - - - - C91197 - - - - - C91199 - - - - - C91200 - - - - - C91206 - - - - - C91227 - - - - - C91229 - - - - - C91231 - - - - - C91244 - - - - - C91250 - - - - - Covance 6329-212 3M T-6316.1 1497 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91255 - - - - - C91258 - - - - - C91264 - - - - - C91269 - - - - - C91270 - - - - - C91274 - - - - - C91289 - - - - - C91294 - - - - - C91297 - - - - - C91305 - - - - - Group: 4 Dose Level: 100 Dosage Unit: ppm C91314 - - - - - C91319 - - - - - C91326 - - - - - C91334 - - - - - C91346 - - - - - C91356 1 1 - - - C91359 - - - - - C91363 - - - - - C91372 - - - - - C91373 - - - - - Covance 6329-212 3M T-6316.1 1498 Appendix 5 Individual Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ PCOAO IU/G -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90719 10 C90725 10 C90732 5 C90753 1 C90775 8 MEAN 7 S.D. 3.8 N 5 Group: 2 Dose Level: 3 Dosage Unit: ppm C90789 6 C90814 7 C90817 7 C90833 12 C90834 6 MEAN 8 S.D. 2.5 N 5 Group: 3 Dose Level: 30 Dosage Unit: ppm C90846 10 C90853 9 C90866 10 C90890 6 C90891 8 MEAN 9 S.D. 1.7 N 5 Covance 6329-212 3M T-6316.1 1499 Appendix 5 Individual Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ PCOAO IU/G -------- Group: 4 Dose Level: 100 Dosage Unit: ppm C90935 6 C90939 9 C90942 5 C90961 4 C90966 6 MEAN 6 S.D. 1.9 N 5 Group: 5 Dose Level: 300 Dosage Unit: ppm C90984 19 C91005 16 C91024 12 C91033 12 C91037 10 MEAN S.D. N 14 3.6 5 Covance 6329-212 3M T-6316.1 1500 Appendix 5 Individual Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ PCOAO IU/G -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91135 1 C91143 0 C91165 7 C91169 3 C91171 4 MEAN 3 S.D. 2.7 N 5 Group: 2 Dose Level: 3 Dosage Unit: ppm C91198 3 C91205 7 C91222 4 C91230 6 C91237 6 MEAN 5 S.D. 1.6 N 5 Group: 3 Dose Level: 30 Dosage Unit: ppm C91251 7 C91272 5 C91285 2 C91287 4 C91291 0 MEAN 4 S.D. 2.7 N 5 Covance 6329-212 3M T-6316.1 1501 Appendix 5 Individual Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ PCOAO IU/G -------- Group: 4 Dose Level: 100 Dosage Unit: ppm C91316 7 C91317 3 C91333 4 C91345 6 C91350 4 MEAN 5 S.D. 1.6 N 5 Group: 5 Dose Level: 300 Dosage Unit: ppm C91423 9 C91427 11 C91430 9 C91443 27 C91448 19 MEAN S.D. N 15 7.9 5 Covance 6329-212 3M T-6316.1 1502 Appendix 5 Individual Clinical Chemistry Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90717 C90718 C90737 C90756 C90769 112 11 .7 6.4 4.4 2.0 .0 44 110 16 1.0 7.4 4.9 2.5 .1 74 105 12 .7 6.4 4.5 1.9 .1 51 108 13 .7 6.4 4.5 1.9 .2 40 96 13 .7 7.1 4.6 2.5 .2 57 MEAN S.D. N 106 13 .8 6.7 4.6 2.2 .1 53 6.3 1.9 .13 .48 .19 .31 .08 13.3 5 5 5 5 5 5 5 5 Group: 5 Dose Level: 300 Dosage Unit: ppm C90971 C90972 C90988 C90996 C91017 100 18 .6 7.0 5.5 1.5 .4 3 93 21 .7 6.7 5.4 1.3 .4 2 92 17 .8 6.4 4.9 1.5 .4 6 86 15 .7 7.2 5.7 1.5 .6 14 101 17 .6 8.0 5.9 2.1 .5 11 MEAN S.D. N 94 18 .7 7.1 5.5 1.6 .5 7 6.2 2.2 .08 .61 .38 .30 .09 5.2 5 5 5 5 5 5 5 5 Covance 6329-212 3M T-6316.1 1503 Appendix 5 Individual Clinical Chemistry Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90717 C90718 C90737 C90756 C90769 96 46 151 36 123 52 90 36 103 39 3 10.7 8.3 147 5.4 104 4 10.7 6.6 148 6.0 102 1 10.4 8.6 146 5.5 101 3 10.5 8.1 150 5.9 106 0 10.2 7.5 148 5.6 104 MEAN S.D. N 113 24.8 5 42 7.0 5 2 10.5 1.6 .21 5 5 7.8 .79 5 148 1.5 5 5.7 .26 5 103 1.9 5 Group: 5 Dose Level: 300 Dosage Unit: ppm C90971 C90972 C90988 C90996 C91017 166 219 256 633 169 250 104 67 202 187 3 10.7 8.0 148 5.4 103 1 10.1 7.8 146 5.8 106 1 10.4 7.9 149 5.4 104 0 10.7 7.3 147 6.1 103 0 10.3 7.3 145 5.4 100 MEAN S.D. N 179 55.6 5 271 213.8 5 1 10.4 1.2 .26 5 5 7.7 .34 5 147 1.6 5 5.6 .32 5 103 2.2 5 Covance 6329-212 3M T-6316.1 1504 Appendix 5 Individual Clinical Chemistry Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ PCOAO IU/G -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90717 10 C90718 3 C90737 12 C90756 11 C90769 9 MEAN 9 S.D. 3.5 N 5 Group: 5 Dose Level: 300 Dosage Unit: ppm C90971 28 C90972 29 C90988 33 C90996 22 C91017 20 MEAN S.D. N 26 5.3 5 Covance 6329-212 3M T-6316.1 1505 Appendix 5 Individual Clinical Chemistry Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91126 C91140 C91145 C91162 C91163 107 17 .9 7.4 5.5 1.9 .3 53 115 11 .8 7.1 5.4 1.7 .3 55 107 13 .6 7.8 5.9 1.9 .3 66 97 13 .8 7.0 5.2 1.8 .1 91 120 12 .7 7.4 5.2 2.2 .3 79 MEAN S.D. N 109 13 .8 7.3 5.4 1.9 .3 69 8.8 2.3 .11 .31 .29 .19 .09 16.2 5 5 5 5 5 5 5 5 Group: 5 Dose Level: 300 Dosage Unit: ppm C91393 C91394 C91404 C91415 C91438 88 23 .8 6.5 5.5 1.0 .4 8 54 17 .9 8.0 6.2 1.8 .4 7 109 22 .7 7.5 5.9 1.6 .3 11 92 20 .7 7.3 6.0 1.3 .5 16 85 27 .9 9.2 7.2 2.0 .3 15 MEAN S.D. N 86 22 .8 7.7 6.2 1.5 .4 11 20.0 3.7 .10 1.00 .63 .40 .08 4.0 5 5 5 5 5 5 5 5 Covance 6329-212 3M T-6316.1 1506 Appendix 5 Individual Clinical Chemistry Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91126 C91140 C91145 C91162 C91163 131 38 115 31 119 33 115 31 166 77 3 11.6 11.4 151 7.0 108 1 10.7 6.3 145 5.6 103 2 11.2 7.7 148 5.8 102 0 10.7 7.3 146 5.5 102 1 11.1 9.3 148 5.7 102 MEAN S.D. N 129 21.6 5 42 19.8 5 1 11.1 1.1 .38 5 5 8.4 1.99 5 148 2.3 5 5.9 .61 5 103 2.6 5 Group: 5 Dose Level: 300 Dosage Unit: ppm C91393 C91394 C91404 C91415 C91438 98 54 120 60 159 104 73 44 273 208 0 10.9 9.2 148 5.7 107 1 11.3 7.3 147 5.4 104 2 10.9 7.6 146 5.7 104 0 10.9 8.4 150 5.2 103 4 11.8 10.3 153 6.1 105 MEAN S.D. N 145 78.4 5 94 67.7 5 1 11.2 1.7 .40 5 5 8.6 1.22 5 149 2.8 5 5.6 .34 5 105 1.5 5 Covance 6329-212 3M T-6316.1 1507 Appendix 5 Individual Clinical Chemistry Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ PCOAO IU/G -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91126 3 C91140 0 C91145 2 C91162 7 C91163 5 MEAN 3 S.D. 2.7 N 5 Group: 5 Dose Level: 300 Dosage Unit: ppm C91393 37 C91394 24 C91404 20 C91415 26 C91438 22 MEAN S.D. N 26 6.6 5 Covance 6329-212 3M T-6316.1 1508 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90716 C90731 C90738 C90745 C90746 C90748 C90760 C90766 C90768 C90780 88 12 .6 7.2 4.9 2.3 .3 112 156 12 .7 7.0 4.7 2.3 .0 76 115 11 .6 7.3 5.1 2.2 .1 78 104 16 .6 7.1 4.7 2.4 .3 64 108 13 .6 7.4 5.1 2.3 .2 91 113 13 .6 8.0 5.2 2.8 .3 77 118 13 .6 7.7 5.0 2.7 .1 64 105 14 .8 7.8 5.0 2.8 .3 87 101 14 .6 7.3 5.0 2.3 .2 92 101 12 1.0 7.3 5.0 2.3 .4 76 MEAN S.D. N 111 13 .7 7.4 5.0 2.4 .2 82 18.0 1.4 .13 .32 .16 .23 .12 14.4 10 10 10 10 10 10 10 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C90791 C90797 C90802 C90807 C90816 C90818 C90820 C90831 C90836 C90840 113 15 .7 6.9 4.5 2.4 .3 56 91 14 .7 7.1 4.7 2.4 .2 52 99 12 .6 7.0 4.8 2.2 .1 57 100 12 .7 7.1 4.9 2.2 .2 59 99 16 .8 7.1 4.8 2.3 .1 60 103 18 .7 6.8 4.6 2.2 .1 60 105 12 .7 6.8 4.6 2.2 .0 68 101 14 .8 7.8 4.9 2.9 .2 76 97 11 .7 7.2 4.9 2.3 .1 62 98 12 .7 7.1 4.7 2.4 .2 58 MEAN S.D. N 101 14 .7 7.1 4.7 2.4 .2 61 5.7 2.2 .06 .28 .14 .21 .08 6.8 10 10 10 10 10 10 10 10 Covance 6329-212 3M T-6316.1 1509 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90843 C90851 C90852 C90856 C90863 C90877 C90880 C90888 C90892 C90897 96 16 .7 7.8 4.9 2.9 .0 44 103 16 .7 6.9 4.9 2.0 .1 52 94 11 .7 6.9 4.6 2.3 .3 55 105 13 .5 7.3 5.1 2.2 .0 47 112 12 .6 7.4 4.6 2.8 .0 70 99 17 .9 7.2 4.8 2.4 .2 52 114 12 .5 6.7 4.5 2.2 .1 64 91 15 .9 7.5 4.9 2.6 .1 48 91 13 .6 6.8 4.7 2.1 .2 52 110 13 .7 6.9 4.8 2.1 .2 50 MEAN S.D. N 102 14 .7 7.1 4.8 2.4 .1 53 8.6 2.0 .14 .36 .18 .31 .10 7.9 10 10 10 10 10 10 10 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C90905 C90907 C90917 C90921 C90934 C90947 C90953 C90957 C90960 C90968 104 14 .7 7.3 5.5 1.8 .2 12 101 15 .7 6.8 4.7 2.1 .0 26 98 13 .6 7.5 5.1 2.4 .2 27 84 14 .7 7.2 5.1 2.1 .2 27 95 17 .6 7.2 5.3 1.9 .2 38 99 17 .6 6.9 5.0 1.9 .1 23 83 14 .6 7.8 5.3 2.5 .2 47 92 12 .6 7.0 4.9 2.1 .3 26 96 16 .7 7.6 5.2 2.4 .2 24 89 14 .6 7.2 4.8 2.4 .1 38 MEAN S.D. N 94 15 .6 7.2 5.1 2.2 .2 29 7.0 1.6 .05 .31 .25 .25 .08 9.8 10 10 10 10 10 10 10 10 Covance 6329-212 3M T-6316.1 1510 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90716 C90731 C90738 C90745 C90746 C90748 C90760 C90766 C90768 C90780 94 34 125 42 80 33 112 43 144 48 138 51 130 47 105 32 158 36 192 46 0 10.7 8.0 147 6.2 104 0 10.6 7.9 150 6.2 105 0 11.0 7.7 149 6.8 107 0 10.7 8.2 148 6.1 105 1 10.6 7.0 147 6.4 105 1 11.2 8.0 150 7.5 105 0 11.2 8.7 147 6.4 105 0 10.5 7.0 150 6.8 104 0 11.1 7.7 152 6.4 108 0 10.0 6.7 149 6.6 105 MEAN S.D. N 128 32.7 10 41 6.9 10 0 .4 10 10.8 .37 10 7.7 .62 10 149 1.7 10 6.5 .41 10 105 1.3 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C90791 C90797 C90802 C90807 C90816 C90818 C90820 C90831 C90836 C90840 137 42 133 33 114 45 111 46 124 36 182 48 106 42 148 50 155 46 90 33 0 10.5 8.2 147 5.9 106 1 10.1 6.5 150 6.3 106 0 10.8 8.0 148 6.2 103 1 10.9 7.8 150 6.0 108 0 10.7 7.6 146 6.1 101 0 10.3 8.5 149 6.2 105 0 10.7 7.4 147 6.6 103 2 10.4 7.6 147 6.6 101 0 10.6 9.1 151 6.3 107 0 11.0 7.6 147 5.7 105 MEAN S.D. N 130 27.0 10 42 6.1 10 0 .7 10 10.6 .28 10 7.8 .69 10 148 1.7 10 6.2 .28 10 104 2.4 10 Covance 6329-212 3M T-6316.1 1511 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90843 C90851 C90852 C90856 C90863 C90877 C90880 C90888 C90892 C90897 188 41 129 49 81 36 103 40 107 40 101 46 146 81 97 40 112 59 98 33 1 11.0 8.5 151 6.6 108 0 10.8 8.0 148 5.8 108 0 10.5 7.2 147 5.7 104 0 10.9 7.3 148 6.2 103 0 11.0 7.5 149 6.6 107 0 10.7 8.5 150 6.2 109 0 10.3 7.2 147 5.8 105 0 10.9 8.2 149 6.4 105 0 10.4 7.1 146 5.8 103 0 11.1 8.0 147 6.0 106 MEAN S.D. N 116 31.0 10 46 14.2 10 0 .3 10 10.8 .28 10 7.8 .55 10 148 1.5 10 6.1 .34 10 106 2.1 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C90905 C90907 C90917 C90921 C90934 C90947 C90953 C90957 C90960 C90968 116 52 72 39 81 49 145 51 121 59 80 33 111 30 157 48 150 66 78 46 4 10.7 8.2 150 5.8 109 0 10.3 8.1 151 6.3 110 0 10.9 7.9 148 6.4 107 0 9.9 7.5 148 6.1 105 0 10.5 7.8 147 6.2 105 0 10.8 7.3 147 6.1 105 0 11.0 7.5 147 5.6 102 0 10.5 8.2 147 6.3 104 0 10.2 7.0 151 6.7 106 1 10.8 7.3 147 5.6 104 MEAN S.D. N 111 32.3 10 47 11.1 10 0 1.3 10 10.6 .35 10 7.7 .42 10 148 1.7 10 6.1 .35 10 106 2.4 10 Covance 6329-212 3M T-6316.1 1512 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91122 C91129 C91148 C91155 C91160 C91174 C91176 C91178 C91181 C91182 105 12 .7 7.4 5.5 1.9 .2 85 128 10 .7 8.3 5.8 2.5 .3 157 118 13 1.0 8.5 6.1 2.4 .1 88 105 15 .7 9.1 6.9 2.2 .3 129 102 13 .7 8.5 6.3 2.2 .4 88 109 13 .9 8.4 6.3 2.1 .2 51 97 14 .7 8.1 5.9 2.2 .1 86 92 15 .9 8.5 6.6 1.9 .3 104 108 13 .9 8.3 5.9 2.4 .2 73 100 14 .9 7.7 5.7 2.0 .1 86 MEAN S.D. N 106 13 .8 8.3 6.1 2.2 .2 95 10.4 1.5 .12 .47 .43 .21 .10 29.6 10 10 10 10 10 10 10 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C91192 C91196 C91204 C91215 C91221 C91223 C91228 C91233 C91241 C91248 91 13 .6 7.3 5.3 2.0 .4 77 112 12 .7 8.0 6.2 1.8 .0 61 103 17 .9 8.1 6.2 1.9 .0 96 110 12 .6 8.1 6.1 2.0 .3 95 108 17 .7 7.5 5.4 2.1 .3 65 113 16 .8 8.3 6.2 2.1 .2 91 109 12 .8 8.3 6.1 2.2 .3 92 121 11 .8 8.4 6.5 1.9 .1 71 108 13 .7 8.4 6.5 1.9 .6 72 102 12 .8 7.7 5.6 2.1 .3 81 MEAN S.D. N 108 14 .7 8.0 6.0 2.0 .2 80 7.9 2.3 .10 .39 .43 .12 .18 12.9 10 10 10 10 10 10 10 10 Covance 6329-212 3M T-6316.1 1513 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91262 C91274 C91281 C91284 C91286 C91288 C91293 C91299 C91304 C91305 101 20 .7 7.7 5.8 1.9 .1 51 95 16 .7 7.7 5.6 2.1 .2 86 127 14 1.0 8.3 6.2 2.1 .4 70 101 11 .7 8.3 6.0 2.3 .2 60 88 20 .6 7.4 5.7 1.7 .2 46 84 23 1.1 8.3 6.2 2.1 .1 101 94 16 .7 6.6 5.0 1.6 .0 55 88 15 .7 8.0 5.7 2.3 .0 57 94 13 .8 8.1 5.9 2.2 .0 73 97 13 .7 7.6 5.4 2.2 .1 89 MEAN S.D. N 97 16 .8 7.8 5.8 2.0 .1 69 11.9 3.8 .16 .53 .37 .24 .13 18.3 10 10 10 10 10 10 10 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C91326 C91327 C91329 C91332 C91337 C91348 C91355 C91370 C91374 C91379 98 24 .7 8.0 6.0 2.0 .1 40 91 25 .8 7.9 6.2 1.7 .1 33 93 15 .7 8.1 5.9 2.2 .2 72 89 18 .6 8.1 6.2 1.9 .4 44 107 18 .7 8.2 6.4 1.8 .1 71 105 24 .8 8.4 6.0 2.4 .1 75 104 14 .8 8.8 6.1 2.7 .2 108 97 19 .9 7.9 6.2 1.7 .1 42 85 14 .5 8.3 6.3 2.0 .4 50 75 15 .8 7.7 5.8 1.9 .2 48 MEAN S.D. N 94 19 .7 8.1 6.1 2.0 .2 58 9.9 4.3 .12 .31 .19 .32 .12 22.9 10 10 10 10 10 10 10 10 Covance 6329-212 3M T-6316.1 1514 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91122 C91129 C91148 C91155 C91160 C91174 C91176 C91178 C91181 C91182 73 22 118 36 118 53 157 87 102 30 161 33 80 28 81 32 100 37 106 36 0 11.4 7.4 146 6.3 107 0 11.6 8.3 151 6.5 108 0 11.8 6.7 153 7.3 108 0 11.0 5.1 148 5.4 107 3 10.8 5.5 147 4.8 105 1 11.3 8.5 151 6.7 107 0 11.0 5.5 146 5.4 104 0 11.9 7.5 148 6.3 104 0 11.5 7.9 150 5.9 107 0 11.1 7.1 147 5.0 102 MEAN S.D. N 110 30.2 10 39 18.5 10 0 1.0 10 11.3 .37 10 7.0 1.22 10 149 2.4 10 6.0 .80 10 106 2.0 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C91192 C91196 C91204 C91215 C91221 C91223 C91228 C91233 C91241 C91248 115 26 72 27 98 28 88 43 103 33 93 36 75 41 103 27 116 36 92 25 0 10.8 6.7 146 6.0 104 0 11.4 5.6 147 6.2 106 0 11.3 6.6 148 5.2 105 0 12.0 8.4 150 6.8 104 0 10.8 7.3 144 5.5 104 1 11.4 8.2 149 6.4 105 0 11.8 7.6 148 7.0 104 1 11.4 7.1 147 6.1 103 1 11.6 6.5 149 5.4 105 0 11.3 8.6 149 6.4 107 MEAN S.D. N 96 14.8 10 32 6.5 10 0 .5 10 11.4 .38 10 7.3 .95 10 148 1.8 10 6.1 .59 10 105 1.2 10 Covance 6329-212 3M T-6316.1 1515 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91262 C91274 C91281 C91284 C91286 C91288 C91293 C91299 C91304 C91305 140 30 112 40 115 37 101 32 126 34 122 27 113 24 135 43 127 34 86 39 0 10.6 7.4 151 6.0 107 1 11.1 6.9 145 5.3 103 0 11.4 6.1 152 5.9 105 0 10.9 7.5 150 5.9 105 1 10.7 7.5 146 5.8 106 1 11.2 5.7 149 6.4 105 0 9.9 6.2 150 5.9 108 0 11.0 8.7 148 5.8 107 0 10.2 5.5 148 5.2 105 0 11.0 6.3 148 6.0 106 MEAN S.D. N 118 16.0 10 34 6.0 10 0 .5 10 10.8 .46 10 6.8 1.00 10 149 2.2 10 5.8 .35 10 106 1.4 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C91326 C91327 C91329 C91332 C91337 C91348 C91355 C91370 C91374 C91379 70 31 75 35 76 27 74 34 76 26 93 31 95 40 85 39 105 37 124 34 2 10.8 6.1 147 6.1 105 0 10.8 6.3 145 5.3 104 3 11.3 7.2 147 6.2 105 0 11.4 6.7 149 6.4 106 0 11.3 6.1 150 5.4 109 4 11.3 6.9 150 6.0 104 2 11.4 6.7 150 5.9 108 1 11.3 8.5 152 5.5 110 0 11.1 7.5 148 6.2 106 0 10.7 6.5 148 5.9 104 MEAN S.D. N 87 17.1 10 33 4.7 10 1 1.5 10 11.1 .27 10 6.8 .74 10 149 2.0 10 5.9 .37 10 106 2.2 10 Covance 6329-212 3M T-6316.1 1516 Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90721 C90735 C90736 C90738 C90739 C90747 C90751 C90752 C90755 C90766 123 11 .6 7.4 4.6 2.8 .2 157 83 11 .7 7.3 4.6 2.7 .0 73 109 11 .4 6.9 4.2 2.7 .1 72 101 10 .6 7.8 5.1 2.7 .1 95 108 13 .5 7.4 4.7 2.7 .3 101 95 11 .6 7.2 4.5 2.7 .3 75 112 11 .6 7.4 4.6 2.8 .0 79 101 11 .7 7.2 4.4 2.8 .0 122 120 11 .7 7.0 4.5 2.5 .1 61 111 14 .9 7.9 4.9 3.0 .3 124 MEAN S.D. N 106 11 .6 7.4 4.6 2.7 .1 96 11.8 1.2 .13 .31 .25 .13 .13 30.3 10 10 10 10 10 10 10 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C90795 C90799 C90802 C90803 C90805 C90806 C90824 C90825 C90839 C90840 95 9 .7 7.7 4.9 2.8 .2 78 105 12 .7 6.9 4.4 2.5 .2 95 102 12 .7 7.1 4.4 2.7 .2 74 123 14 .8 7.8 5.4 2.4 .2 112 115 12 .6 7.6 4.8 2.8 .3 82 91 13 .4 6.7 4.1 2.6 .3 58 98 13 .6 7.8 5.0 2.8 .2 88 109 13 .7 7.6 4.9 2.7 .1 106 114 12 .6 7.5 5.1 2.4 .0 111 128 16 .8 6.8 4.5 2.3 .0 59 MEAN S.D. N 108 13 .7 7.4 4.8 2.6 .2 86 12.1 1.8 .12 .43 .39 .19 .11 19.8 10 10 10 10 10 10 10 10 Covance 6329-212 3M T-6316.1 1517 Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90847 C90855 C90858 C90859 C90864 C90865 C90871 C90872 C90882 C90886 102 14 .7 6.7 4.4 2.3 .2 47 101 14 .6 6.6 4.1 2.5 .2 42 86 16 .8 8.0 5.2 2.8 .0 102 96 13 .8 7.9 5.1 2.8 .0 67 110 14 .7 6.9 4.3 2.6 .1 60 96 13 .6 7.6 5.2 2.4 .1 64 100 13 .8 6.8 4.6 2.2 .1 89 98 11 .5 6.9 4.5 2.4 .1 86 101 12 .6 7.4 4.8 2.6 .3 60 95 10 .7 6.9 4.4 2.5 .0 68 MEAN S.D. N 98 13 .7 7.2 4.7 2.5 .1 68 6.2 1.7 .10 .51 .39 .20 .10 18.8 10 10 10 10 10 10 10 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C90916 C90931 C90937 C90940 C90944 C90945 C90954 C90956 C90957 C90963 97 14 .6 7.4 5.2 2.2 .1 40 97 13 .6 7.7 5.4 2.3 .0 35 98 14 .7 7.3 4.8 2.5 .2 60 96 13 .6 7.8 5.3 2.5 .0 43 100 18 .6 8.1 5.8 2.3 .0 69 92 14 .5 7.2 5.2 2.0 .2 19 86 15 .6 7.6 5.3 2.3 .2 35 79 16 .8 7.9 5.3 2.6 .0 42 104 16 .4 7.9 5.3 2.6 .2 22 99 14 .7 8.1 5.1 3.0 .3 59 MEAN S.D. N 95 15 .6 7.7 5.3 2.4 .1 42 7.3 1.6 .11 .32 .25 .28 .11 16.2 10 10 10 10 10 10 10 10 Covance 6329-212 3M T-6316.1 1518 Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90721 C90735 C90736 C90738 C90739 C90747 C90751 C90752 C90755 C90766 94 43 91 41 118 30 84 35 103 31 108 34 110 77 165 35 163 40 109 31 0 11.4 8.2 148 7.0 105 1 11.3 8.2 148 6.5 105 1 10.3 6.1 148 6.2 105 4 10.8 6.8 150 6.7 108 1 10.6 6.3 151 6.3 106 0 10.5 7.6 147 5.9 106 2 11.2 8.3 148 7.4 107 3 10.7 7.8 149 7.3 106 0 10.5 7.7 147 6.5 106 1 10.6 6.7 153 6.9 108 MEAN S.D. N 114 28.0 10 40 13.8 10 1 1.3 10 10.8 .38 10 7.4 .82 10 149 1.9 10 6.7 .48 10 106 1.1 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C90795 C90799 C90802 C90803 C90805 C90806 C90824 C90825 C90839 C90840 99 37 89 33 117 47 215 101 121 43 142 30 130 37 97 33 96 31 87 33 1 10.3 6.0 146 5.8 104 0 10.2 6.3 146 6.0 104 1 10.5 7.0 146 6.5 104 2 11.2 7.5 152 7.0 107 0 10.6 6.8 147 6.3 105 0 10.1 6.7 147 6.2 108 0 10.5 7.0 143 5.4 100 0 11.2 7.7 148 6.8 105 0 10.9 7.9 147 6.2 105 0 10.5 6.1 148 5.8 108 MEAN S.D. N 119 38.3 10 42 21.2 10 0 .7 10 10.6 .39 10 6.9 .66 10 147 2.3 10 6.2 .48 10 105 2.4 10 Covance 6329-212 3M T-6316.1 1519 Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90847 C90855 C90858 C90859 C90864 C90865 C90871 C90872 C90882 C90886 84 52 82 48 123 58 116 37 142 83 84 36 209 136 90 40 102 39 108 44 0 10.8 7.2 148 6.7 109 3 10.5 7.3 146 5.7 106 0 11.1 7.1 148 6.5 105 0 10.6 7.0 148 6.1 105 0 10.4 6.7 146 6.4 106 1 10.9 7.2 149 6.5 105 1 10.3 7.2 147 6.1 107 0 10.0 6.8 145 5.9 106 0 10.7 7.2 146 6.7 105 1 11.0 8.2 145 6.8 106 MEAN S.D. N 114 38.7 10 57 31.0 10 1 1.0 10 10.6 .34 10 7.2 .40 10 147 1.4 10 6.3 .37 10 106 1.2 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C90916 C90931 C90937 C90940 C90944 C90945 C90954 C90956 C90957 C90963 87 63 113 74 710 414 129 54 91 91 101 40 99 39 78 60 130 43 116 57 1 10.1 6.8 149 5.9 108 0 10.9 7.2 145 6.4 105 2 10.4 7.3 146 5.9 107 1 10.9 7.3 149 6.4 105 0 10.7 6.6 146 6.1 105 0 10.4 7.3 148 6.3 107 0 11.1 7.5 148 6.7 105 1 10.7 7.7 147 6.0 106 2 10.8 7.9 149 7.0 106 1 11.1 6.9 147 6.4 105 MEAN S.D. N 165 192.1 10 94 113.7 10 1 .8 10 10.7 .32 10 7.2 .40 10 147 1.4 10 6.3 .35 10 106 1.1 10 Covance 6329-212 3M T-6316.1 1520 Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91127 C91128 C91133 C91137 C91144 C91153 C91154 C91158 C91161 C91183 110 14 .5 8.1 6.0 2.1 .3 82 100 12 1.0 7.9 5.9 2.0 .2 103 98 15 .9 8.7 6.0 2.7 .2 93 102 15 .7 7.7 5.7 2.0 .3 72 88 14 .8 7.1 5.1 2.0 .2 100 86 13 .6 8.4 6.3 2.1 .3 96 96 16 .7 7.5 5.3 2.2 .0 75 93 13 .4 7.8 5.6 2.2 .1 103 105 17 .8 7.5 5.2 2.3 .2 62 133 25 1.2 9.3 6.8 2.5 .1 82 MEAN S.D. N 101 15 .8 8.0 5.8 2.2 .2 87 13.4 3.7 .24 .65 .53 .23 .10 14.3 10 10 10 10 10 10 10 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C91196 C91207 C91210 C91211 C91214 C91221 C91224 C91235 C91243 C91244 118 15 .6 8.2 6.1 2.1 .1 76 99 15 .7 8.0 5.8 2.2 .2 125 109 13 .7 8.4 6.4 2.0 .2 110 101 13 .8 7.7 5.3 2.4 .1 124 107 14 .7 7.4 4.8 2.6 .0 121 100 17 1.0 7.9 5.2 2.7 .3 73 104 19 .7 8.1 5.7 2.4 .4 91 93 12 .6 8.2 6.1 2.1 .0 80 95 13 .7 8.8 6.1 2.7 .4 115 105 13 .7 9.3 6.6 2.7 .2 123 MEAN S.D. N 103 14 .7 8.2 5.8 2.4 7.3 2.2 .11 .54 .57 .28 10 10 10 10 10 10 .2 104 .14 21.4 10 10 Covance 6329-212 3M T-6316.1 1521 Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91252 C91254 C91264 C91267 C91283 C91286 C91292 C91295 C91301 C91303 104 13 .7 7.7 5.5 2.2 .2 78 91 20 .7 7.3 5.3 2.0 .2 72 99 16 .5 8.0 5.6 2.4 .2 95 111 14 .6 8.8 6.3 2.5 .3 119 121 11 .6 8.0 5.8 2.2 .3 80 101 23 .7 7.3 5.7 1.6 .1 59 116 19 .7 8.6 6.3 2.3 .1 90 114 14 .5 9.0 6.5 2.5 .5 109 108 16 .6 7.6 5.7 1.9 .2 93 107 14 .6 7.6 5.4 2.2 .1 60 MEAN S.D. N 107 16 .6 8.0 5.8 2.2 .2 86 8.9 3.7 .08 .61 .41 .28 .12 19.6 10 10 10 10 10 10 10 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C91312 C91323 C91328 C91339 C91340 C91351 C91354 C91357 C91369 C91371 89 15 .7 9.2 6.7 2.5 .2 58 88 26 .8 9.1 6.6 2.5 .3 61 92 16 1.0 9.2 6.4 2.8 .4 75 89 15 .6 8.4 6.1 2.3 .0 85 93 23 .9 8.4 6.0 2.4 .3 41 79 15 .7 8.0 6.0 2.0 .0 93 97 23 .9 8.1 5.9 2.2 .2 77 107 37 1.3 8.3 6.2 2.1 .1 48 90 21 .7 7.9 5.8 2.1 .1 61 91 17 .7 8.8 6.2 2.6 .3 107 MEAN S.D. N 92 21 .8 8.5 6.2 2.4 .2 71 7.1 7.0 .21 .50 .30 .25 .14 20.6 10 10 10 10 10 10 10 10 Covance 6329-212 3M T-6316.1 1522 Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91127 C91128 C91133 C91137 C91144 C91153 C91154 C91158 C91161 C91183 102 39 101 23 97 31 101 45 183 47 116 40 109 30 141 64 110 31 164 88 0 10.8 5.6 147 5.6 108 0 10.7 6.2 146 5.4 104 0 11.2 5.4 151 6.1 105 0 10.9 5.9 148 6.1 107 0 10.9 6.7 146 6.5 106 0 11.3 6.4 145 5.8 102 0 10.6 6.7 148 7.1 107 2 10.7 5.8 142 5.5 102 1 10.6 5.9 145 6.3 106 3 11.9 5.9 152 6.8 105 MEAN S.D. N 122 30.0 10 44 19.3 10 1 1.1 10 11.0 .41 10 6.0 .44 10 147 2.9 10 6.1 .57 10 105 2.0 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C91196 C91207 C91210 C91211 C91214 C91221 C91224 C91235 C91243 C91244 75 30 1 11.0 4.7 146 5.7 104 80 37 1 11.0 5.4 146 5.4 106 104 43 0 11.7 7.1 148 6.8 106 70 30 0 10.9 6.4 147 6.2 107 153 83 15 10.7 6.4 146 6.3 106 109 29 0 10.5 5.0 146 5.6 106 100 28 0 10.6 5.4 148 5.3 104 110 28 0 11.2 6.2 146 5.6 103 109 48 0 11.0 5.8 147 5.2 105 105 57 1 11.7 5.1 148 5.2 103 MEAN S.D. N 102 23.6 10 41 17.7 10 2 4.7 10 11.0 .41 10 5.8 .76 10 147 .9 10 5.7 .54 10 105 1.4 10 Covance 6329-212 3M T-6316.1 1523 Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91252 C91254 C91264 C91267 C91283 C91286 C91292 C91295 C91301 C91303 77 25 141 31 130 48 79 30 94 42 114 34 112 56 110 50 78 30 106 28 2 10.7 5.2 146 5.4 105 0 10.8 6.1 147 6.1 107 3 10.8 6.1 146 5.7 106 0 11.1 4.7 145 5.6 105 1 11.1 6.1 148 5.9 108 1 10.4 6.3 146 6.3 109 3 11.4 6.8 148 6.9 106 1 12.3 8.0 150 7.6 106 0 10.7 5.3 149 5.5 108 4 10.8 6.3 145 6.2 107 MEAN S.D. N 104 22.1 10 37 10.8 10 2 1.4 10 11.0 .53 10 6.1 .92 10 147 1.7 10 6.1 .69 10 107 1.3 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C91312 C91323 C91328 C91339 C91340 C91351 C91354 C91357 C91369 C91371 61 26 122 69 52 26 78 42 92 34 144 59 91 52 112 36 104 48 197 76 1 11.3 5.7 144 4.9 100 0 11.0 4.9 146 4.6 104 0 11.2 6.5 150 5.6 104 1 11.4 6.1 146 5.6 104 2 10.9 5.9 146 6.2 107 2 11.4 6.5 145 6.1 103 0 10.8 6.0 146 6.4 106 4 11.0 7.2 148 6.8 106 0 11.2 6.1 146 6.3 106 2 11.5 6.8 146 5.2 102 MEAN S.D. N 105 42.4 10 47 17.3 10 1 1.3 10 11.2 .24 10 6.2 .63 10 146 1.6 10 5.8 .71 10 104 2.1 10 Covance 6329-212 3M T-6316.1 1524 Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90712 C90723 C90727 C90730 C90755 C90758 C90759 C90764 C90766 C90774 98 12 .8 7.2 5.0 2.2 .2 96 126 10 .7 7.7 4.5 3.2 .0 120 122 14 .7 7.5 4.3 3.2 .1 164 96 14 .6 7.4 4.3 3.1 .2 247 134 11 .5 7.0 4.4 2.6 .1 80 114 14 .7 7.2 4.3 2.9 .2 124 115 13 .6 7.3 4.6 2.7 .0 121 112 15 .7 6.9 4.1 2.8 .0 102 111 12 .7 7.9 4.2 3.7 .3 296 119 13 .5 6.6 4.3 2.3 .1 107 MEAN S.D. N 115 13 .6 7.3 4.4 2.9 11.7 1.5 .10 .38 .25 .45 10 10 10 10 10 10 .1 146 .10 70.8 10 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C90781 C90792 C90793 C90798 C90799 C90800 C90812 C90822 C90829 C90832 106 12 .7 8.1 4.9 3.2 .0 141 128 12 .5 7.0 4.5 2.5 .2 107 97 14 .7 8.7 4.8 3.9 .3 180 117 9 .8 7.8 4.5 3.3 .2 110 100 13 .7 8.6 6.0 2.6 .0 98 117 10 .6 6.8 4.6 2.2 .0 78 114 14 .6 7.3 4.4 2.9 .2 119 104 12 .5 7.1 4.4 2.7 .1 179 125 12 .8 7.2 4.4 2.8 .2 88 101 13 .6 7.6 4.5 3.1 .4 94 MEAN S.D. N 111 12 .6 7.6 4.7 2.9 10.8 1.6 .11 .67 .49 .48 10 10 10 10 10 10 .2 119 .13 36.1 10 10 Covance 6329-212 3M T-6316.1 1525 Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90841 C90850 C90854 C90855 C90856 C90859 C90873 C90879 C90881 C90896 113 12 .8 7.1 4.6 2.5 .3 52 102 17 .5 7.0 4.7 2.3 .1 93 128 18 .8 7.2 4.7 2.5 .0 45 107 15 .4 7.0 4.5 2.5 .4 65 108 14 .4 7.2 4.9 2.3 .2 66 123 15 .8 7.6 5.0 2.6 .0 101 116 13 .8 7.4 4.7 2.7 .3 101 128 14 .7 7.8 4.5 3.3 .1 176 110 11 .7 7.7 4.5 3.2 .1 107 124 12 .9 6.9 4.5 2.4 .1 116 MEAN S.D. N 116 14 .7 7.3 4.7 2.6 .2 92 9.4 2.2 .18 .32 .18 .35 .13 38.3 10 10 10 10 10 10 10 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C90908 C90914 C90923 C90926 C90927 C90934 C90936 C90944 C90946 C90956 123 15 .5 8.0 5.3 2.7 .0 41 118 15 .7 7.9 5.7 2.2 .0 77 132 14 .7 7.6 4.9 2.7 .2 46 119 13 .6 7.1 4.8 2.3 .1 31 126 14 .6 7.5 5.4 2.1 .0 63 108 15 .7 7.7 5.3 2.4 .2 39 112 13 .5 7.6 5.0 2.6 .1 46 105 15 .4 7.8 5.6 2.2 .2 91 95 16 .7 7.3 5.2 2.1 .2 23 89 14 .6 8.3 5.5 2.8 .1 35 MEAN S.D. N 113 14 .6 7.7 5.3 2.4 .1 49 13.7 1.0 .11 .35 .30 .27 .09 21.4 10 10 10 10 10 10 10 10 Covance 6329-212 3M T-6316.1 1526 Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90712 C90723 C90727 C90730 C90755 C90758 C90759 C90764 C90766 C90774 107 33 109 47 86 36 124 59 130 48 159 39 122 38 110 31 144 45 83 28 2 11.0 6.9 151 6.7 112 1 11.3 6.0 150 6.3 108 2 11.2 6.3 148 6.8 106 0 11.1 6.0 148 6.1 107 0 11.8 8.2 150 5.8 107 7 11.1 7.0 152 6.7 108 7 11.0 7.1 148 6.7 108 7 10.6 6.5 151 6.4 110 9 11.3 6.5 152 6.3 108 1 10.3 4.9 148 5.9 113 MEAN S.D. N 117 23.7 10 40 9.4 10 4 3.5 10 11.1 .41 10 6.5 .87 10 150 1.7 10 6.4 .36 10 109 2.3 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C90781 C90792 C90793 C90798 C90799 C90800 C90812 C90822 C90829 C90832 80 44 81 42 110 57 129 39 114 45 120 45 104 42 88 34 96 42 153 85 1 11.2 6.9 152 6.7 108 0 10.6 6.3 150 6.7 110 6 11.5 7.9 153 6.8 107 6 11.0 6.7 148 7.1 105 0 11.3 5.9 149 5.4 108 7 10.3 6.4 150 6.1 108 3 10.9 6.8 151 5.9 107 4 10.8 6.5 151 6.8 108 4 11.0 6.8 148 6.5 108 0 11.2 7.0 150 6.8 109 MEAN S.D. N 108 22.9 10 48 14.4 10 3 2.7 10 11.0 .35 10 6.7 .53 10 150 1.6 10 6.5 .52 10 108 1.3 10 Covance 6329-212 3M T-6316.1 1527 Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90841 C90850 C90854 C90855 C90856 C90859 C90873 C90879 C90881 C90896 133 73 0 10.9 6.3 151 6.9 112 112 37 8 10.6 5.9 148 6.4 109 109 35 0 10.4 6.3 148 6.6 111 148 82 8 10.7 7.4 151 6.6 108 109 56 0 11.0 6.2 150 6.2 109 79 38 5 11.2 7.0 149 5.8 107 125 76 10 11.1 7.9 151 6.5 110 76 40 0 11.3 6.8 152 7.3 110 75 30 0 11.2 6.9 151 6.2 109 88 44 10 10.7 7.2 146 6.2 107 MEAN S.D. N 105 25.4 10 51 19.2 10 4 4.5 10 10.9 .30 10 6.8 .62 10 150 1.9 10 6.5 .42 10 109 1.6 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C90908 C90914 C90923 C90926 C90927 C90934 C90936 C90944 C90946 C90956 87 110 94 53 79 72 73 36 85 54 110 116 104 69 68 55 57 43 76 70 5 10.9 6.2 147 6.2 109 8 10.7 6.4 146 6.4 110 0 11.4 6.7 147 7.2 109 7 10.7 5.9 146 5.8 108 3 11.0 6.4 148 5.8 108 13 10.9 6.3 149 6.6 113 11 10.8 6.5 146 5.9 107 7 11.0 7.0 148 6.1 107 6 10.9 7.1 149 6.6 109 0 10.9 6.2 151 6.6 112 MEAN S.D. N 83 16.2 10 68 26.5 10 6 4.2 10 10.9 .20 10 6.5 .37 10 148 1.6 10 6.3 .45 10 109 2.0 10 Covance 6329-212 3M T-6316.1 1528 Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91123 C91125 C91131 C91132 C91153 C91156 C91158 C91178 C91180 C91189 108 12 .7 7.9 5.9 2.0 .2 91 107 12 .9 8.5 6.2 2.3 .2 136 110 14 .8 7.6 5.3 2.3 .4 86 115 8 .6 7.5 5.5 2.0 .2 126 124 12 .6 7.7 5.9 1.8 .5 83 88 16 .6 7.9 5.7 2.2 .0 112 119 13 .6 8.5 6.0 2.5 .4 144 105 11 .9 8.9 6.6 2.3 .1 132 81 18 .7 6.8 5.1 1.7 .4 128 122 17 .8 9.1 7.0 2.1 .4 125 MEAN S.D. N 108 13 .7 8.0 5.9 2.1 14.0 3.0 .12 .70 .58 .25 10 10 10 10 10 10 .3 116 .16 22.1 10 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C91194 C91197 C91199 C91200 C91206 C91227 C91229 C91231 C91244 C91250 107 13 .6 7.4 4.7 2.7 .3 95 126 14 .7 7.6 5.3 2.3 .1 140 113 13 .9 8.3 6.1 2.2 .2 100 91 11 .6 7.9 5.5 2.4 .0 71 89 13 .7 8.6 5.3 3.3 .1 197 117 12 .4 8.8 6.7 2.1 .3 112 104 11 .8 8.5 6.1 2.4 .0 111 116 13 .8 8.0 5.8 2.2 .1 72 103 11 .8 9.2 6.2 3.0 .0 144 88 16 .4 8.1 5.9 2.2 .6 151 MEAN S.D. N 105 13 .7 8.2 5.8 2.5 13.0 1.6 .17 .55 .57 .40 10 10 10 10 10 10 .2 119 .19 39.1 10 10 Covance 6329-212 3M T-6316.1 1529 Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91255 76 16 .8 8.5 6.1 2.4 .3 61 C91258 99 12 .8 9.0 6.0 3.0 .2 94 C91264 100 13 .7 8.3 5.8 2.5 .2 102 C91269 95 15 .8 7.8 5.6 2.2 .3 72 C91270 93 18 .9 8.4 6.2 2.2 .1 94 C91274 SH 99 12 .9 8.5 6.1 2.4 .9 124 C91289 94 13 .6 8.8 5.9 2.9 .1 92 C91294 83 15 .8 8.1 5.5 2.6 .0 60 C91297 92 11 .8 7.9 6.0 1.9 .4 115 C91305 99 13 .8 8.2 5.9 2.3 .2 126 MEAN S.D. N 93 14 .8 8.4 5.9 2.4 .3 94 7.8 2.1 .09 .37 .22 .33 .25 23.9 10 10 10 10 10 10 10 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C91314 C91319 C91326 C91334 C91346 C91356 C91359 C91363 C91372 C91373 88 26 .6 8.4 6.4 2.0 .3 65 101 15 .8 8.0 5.7 2.3 .3 86 86 15 .7 8.0 5.8 2.2 .0 69 122 14 .6 7.9 5.7 2.2 .2 68 107 13 .5 8.9 6.1 2.8 .2 79 98 21 .6 9.1 5.9 3.2 .0 144 110 16 .7 8.5 5.9 2.6 .0 83 113 17 .7 8.5 5.8 2.7 .1 96 109 12 .6 8.7 5.9 2.8 .1 104 96 15 .6 8.9 6.1 2.8 .2 89 MEAN S.D. N 103 16 .6 8.5 5.9 2.6 .1 88 11.3 4.2 .08 .42 .22 .37 .12 23.2 10 10 10 10 10 10 10 10 Covance 6329-212 3M T-6316.1 1530 Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91123 C91125 C91131 C91132 C91153 C91156 C91158 C91178 C91180 C91189 116 63 80 41 113 36 112 33 63 31 85 31 130 51 85 32 184 80 106 36 3 11.0 5.2 147 5.7 107 1 11.5 5.6 148 5.6 106 0 10.9 5.6 151 5.6 111 0 10.2 4.6 148 5.7 109 4 11.2 6.3 145 6.7 106 4 10.8 5.6 147 5.1 106 4 11.4 6.0 148 5.1 102 0 11.5 5.9 148 6.1 107 0 10.6 6.0 144 5.0 101 2 11.5 7.4 151 5.6 100 MEAN S.D. N 107 33.7 10 43 16.5 10 2 1.8 10 11.1 .44 10 5.8 .73 10 148 2.2 10 5.6 .51 10 106 3.5 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C91194 C91197 C91199 C91200 C91206 C91227 C91229 C91231 C91244 C91250 131 44 116 69 84 45 95 29 84 40 101 66 97 35 68 22 164 95 97 39 0 10.3 5.9 148 6.4 111 1 10.8 5.1 150 6.3 110 7 11.6 5.9 148 5.9 106 1 11.5 6.2 151 6.6 109 7 11.6 6.5 150 5.3 102 1 11.6 5.9 148 6.6 107 0 11.0 5.5 146 5.9 106 4 11.1 6.0 149 6.2 111 0 11.5 5.2 150 5.4 107 0 11.2 5.5 146 6.0 109 MEAN S.D. N 104 27.4 10 48 22.0 10 2 2.8 10 11.2 .43 10 5.8 .44 10 149 1.7 10 6.1 .45 10 108 2.8 10 Covance 6329-212 3M T-6316.1 1531 Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91255 138 54 C91258 105 48 C91264 143 49 C91269 118 43 C91270 84 35 C91274 SH 275 270 C91289 119 37 C91294 100 46 C91297 77 30 C91305 92 52 2 10.6 6.2 149 5.0 103 1 11.2 5.9 151 6.0 107 0 10.8 5.5 148 5.6 109 2 10.8 5.7 145 5.2 105 23 11.9 7.4 155 5.8 108 0 10.6 5.0 145 6.3 107 3 11.4 7.7 157 6.5 110 7 11.5 6.4 150 6.8 111 7 11.2 5.5 148 5.6 107 5 11.2 6.2 150 5.8 106 MEAN S.D. N 125 56.9 10 66 72.0 10 5 6.8 10 11.1 .42 10 6.2 .85 10 150 3.9 10 5.9 .56 10 107 2.4 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C91314 C91319 C91326 C91334 C91346 C91356 C91359 C91363 C91372 C91373 81 57 143 125 86 41 92 83 82 73 95 53 74 41 82 46 78 43 79 67 0 11.3 5.7 148 6.0 110 4 11.2 5.9 146 6.2 107 9 10.7 6.2 145 4.9 101 7 11.2 6.2 148 5.6 107 0 11.3 6.3 147 5.9 108 0 11.8 6.2 146 5.4 105 0 11.1 5.0 149 6.0 110 9 11.3 5.1 143 6.0 109 1 11.3 5.6 149 5.6 109 8 11.6 5.4 147 5.3 104 MEAN S.D. N 89 19.9 10 63 26.1 10 4 4.0 10 11.3 .29 10 5.8 .48 10 147 1.9 10 5.7 .40 10 107 2.9 10 Covance 6329-212 3M T-6316.1 1532 Appendix 5 Individual Clinical Chemistry Data Males Week 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ CHOL MG/DL -------- TRIG MG/DL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90712 C90716 C90722 C90724 C90726 C90728 C90735 C90742 C90743 C90747 C90750 C90758 C90765 C90778 293 115 212 270 87 156 121 268 102 70 108 97 132 141 114 98 260 508 114 171 84 130 187 97 182 325 138 194 MEAN S.D. N 152 65.0 14 189 119.3 14 Covance 6329-212 3M T-6316.1 1533 Appendix 5 Individual Clinical Chemistry Data Males Week 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ CHOL MG/DL -------- TRIG MG/DL -------- Group: 2 Dose Level: 3 Dosage Unit: ppm C90781 C90782 C90783 C90785 C90787 C90788 C90791 C90794 C90799 C90804 C90809 C90810 C90811 C90812 C90815 C90823 C90829 C90830 C90832 C90835 C90838 C90840 212 616 177 188 113 153 100 151 59 47 68 128 110 231 82 64 161 177 170 148 75 129 215 249 108 167 136 171 120 85 126 201 89 130 96 36 138 157 418 356 297 686 94 117 MEAN S.D. N 144 83.4 22 199 162.2 22 Covance 6329-212 3M T-6316.1 1534 Appendix 5 Individual Clinical Chemistry Data Males Week 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ CHOL MG/DL -------- TRIG MG/DL -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90842 C90844 C90850 C90851 C90854 C90855 C90856 C90858 C90860 C90864 C90865 C90867 C90869 C90871 C90881 C90882 C90883 C90884 C90885 C90889 C90893 C90900 107 270 85 41 78 85 86 147 47 83 97 109 186 343 135 113 73 68 114 157 84 163 97 146 143 85 119 160 143 129 99 54 96 160 105 109 75 38 160 187 156 56 108 91 MEAN S.D. N 109 33.4 22 127 73.2 22 Covance 6329-212 3M T-6316.1 1535 Appendix 5 Individual Clinical Chemistry Data Males Week 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ CHOL MG/DL -------- TRIG MG/DL -------- Group: 4 Dose Level: 100 Dosage Unit: ppm C90904 C90908 C90910 C90911 C90916 C90926 C90927 C90928 C90933 C90944 C90947 C90948 C90953 C90955 C90956 C90965 C90968 56 59 60 86 206 389 16 20 67 48 53 200 164 276 65 27 58 59 88 39 61 62 82 64 145 303 71 75 105 94 34 26 50 44 MEAN S.D. N 81 48.7 17 110 111.2 17 Group: 6 Dose Level: 100 Dosage Unit: ppm C91047 C91054 C91056 C91060 C91065 C91070 C91071 C91072 C91073 C91076 114 221 95 127 60 97 113 189 191 469 96 113 279 251 103 118 113 91 37 56 MEAN S.D. N 120 68.7 10 173 120.7 10 1536 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Clinical Chemistry Data Females Week 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ CHOL MG/DL -------- TRIG MG/DL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91127 C91131 C91138 C91144 C91148 C91157 C91159 C91166 C91172 C91176 C91183 C91188 C91190 72 178 86 35 152 144 107 53 99 353 128 175 110 244 122 296 116 89 101 62 103 195 109 107 203 229 MEAN S.D. N 116 32.6 13 166 97.3 13 Group: 2 Dose Level: 3 Dosage Unit: ppm C91197 C91201 C91207 C91211 C91216 C91220 C91221 C91227 C91232 C91235 C91236 C91242 C91243 108 438 121 68 128 42 126 61 75 141 131 120 117 217 124 184 137 428 70 188 109 208 60 66 113 117 MEAN S.D. N 109 24.9 13 175 128.4 13 1537 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Clinical Chemistry Data Females Week 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ CHOL MG/DL -------- TRIG MG/DL -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91252 C91253 C91254 C91256 C91264 C91278 C91279 C91286 C91292 C91294 C91295 C91301 C91303 C91307 C91310 99 88 89 315 105 47 101 97 86 53 95 86 86 114 74 45 129 367 85 49 166 136 110 66 79 138 104 32 93 106 MEAN S.D. N 100 22.8 15 116 97.6 15 Covance 6329-212 3M T-6316.1 1538 Appendix 5 Individual Clinical Chemistry Data Females Week 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ CHOL MG/DL -------- TRIG MG/DL -------- Group: 4 Dose Level: 100 Dosage Unit: ppm C91315 C91324 C91327 C91332 C91335 C91336 C91338 C91340 C91341 C91342 C91356 C91357 C91360 C91361 C91362 C91372 C91376 C91378 C91380 126 91 90 61 153 289 90 18 86 54 93 30 63 41 69 27 104 56 88 62 254 213 77 53 70 43 128 68 150 332 88 66 103 66 74 41 140 97 MEAN S.D. N 108 44.8 19 90 88.3 19 Covance 6329-212 3M T-6316.1 1539 Appendix 5 Individual Clinical Chemistry Data Females Week 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ CHOL MG/DL -------- TRIG MG/DL -------- Group: 6 Dose Level: 100 Dosage Unit: ppm C91451 C91453 C91455 C91459 C91462 C91467 C91473 C91479 C91480 C91485 C91487 C91488 118 160 111 1350 102 586 213 391 150 87 171 131 128 396 284 1525 150 386 96 154 179 314 142 305 MEAN S.D. N 154 53.4 12 482 469.7 12 Covance 6329-212 3M T-6316.1 1540 Appendix 5 Individual Clinical Urinalysis Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90717 C90718 C90737 C90756 C90769 7.5 1.029 7.0 8.6 1.027 6.5 14.3 1.020 7.5 31.8 1.010 7.0 25.2 1.012 7.0 MEAN S.D. N 17.5 10.64 5 1.020 .0086 5 7.0 .35 5 Group: 5 Dose Level: 300 Dosage Unit: ppm C90971 C90972 C90988 C90996 C91017 22.1 1.007 7.0 27.0 1.009 7.0 46.5 a 1.005 7.0 28.6 1.012 7.0 18.2 1.017 6.5 MEAN S.D. N 28.5 10.88 5 1.010 .0047 5 6.9 .22 5 a Sample was overflowing at the time of collection. Covance 6329-212 3M T-6316.1 1541 Appendix 5 Individual Clinical Urinalysis Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90717 30 - ++ - - - C90718 30 - - - + - C90737 30 - + - - - C90756 10 - - - - - C90769 30 - - - - - Group: 5 Dose Level: 300 Dosage Unit: ppm C90971 30 - ++ - - - C90972 30 - - - - - C90988 10 - - - - - C90996 30 - + - - - C91017 30 - +++ - + - Covance 6329-212 3M T-6316.1 1542 Appendix 5 Individual Clinical Urinalysis Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90717 0 1 1 2 0 4D CJ Q C90718 1 1 1 1 0 4D BJ PQ C90737 1 0 1 1 0 3D BJ PQ C90756 0 1 1 0 0 2D BJ Q C90769 0 0 1 1 0 4D BJ PQ Group: 5 Dose Level: 300 Dosage Unit: ppm C90971 0 0 1 1 0 0 AJ Q C90972 0 0 1 1 0 3D BJ Q C90988 0 1 1 1 0 1B1D AJ PQ C90996 0 1 1 1 0 D BJ PQ C91017 0 0 1 1 0 3D BJ PQ Covance 6329-212 3M T-6316.1 1543 Appendix 5 Individual Clinical Urinalysis Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91126 C91140 C91145 C91162 C91163 26.0 1.006 7.0 46.2 a 1.003 6.5 20.0 1.006 7.0 5.0 1.018 7.0 2.3 1.027 6.5 MEAN S.D. N 19.9 17.75 5 1.012 .0102 5 6.8 .27 5 Group: 5 Dose Level: 300 Dosage Unit: ppm C91393 C91394 C91404 C91415 C91438 21.3 1.008 7.5 19.0 1.011 6.5 14.1 1.015 6.5 10.8 1.013 6.5 43.0 a 1.002 6.5 MEAN S.D. N 21.6 12.63 5 1.010 .0051 5 6.7 .45 5 a Sample was overflowing at the time of collection. Covance 6329-212 3M T-6316.1 1544 Appendix 5 Individual Clinical Urinalysis Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91126 0 - - - - - C91140 0 - - - - - C91145 10 - - - - - C91162 10 - - - - - C91163 10 - - - - - Group: 5 Dose Level: 300 Dosage Unit: ppm C91393 0 - - - - - C91394 0 - ++ - - - C91404 0 - - - - - C91415 0 - - - - - C91438 0 - - - - - Covance 6329-212 3M T-6316.1 1545 Appendix 5 Individual Clinical Urinalysis Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91126 0 0 1 1 0 1D AJ - C91140 0 0 1 1 0 0 AJ - C91145 0 1 1 1 0 0 AJ - C91162 0 0 1 1 0 1B1D BJ - C91163 0 0 1 1 0 3D CJ - Group: 5 Dose Level: 300 Dosage Unit: ppm C91393 0 0 1 1 0 0 BJ - C91394 0 0 0 1 0 0 BJ - C91404 0 0 0 1 0 1B BJ - C91415 0 0 1 1 0 0 BJ - C91438 0 1 1 1 0 1B AJ - Covance 6329-212 3M T-6316.1 1546 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90716 C90731 C90738 C90745 C90746 C90748 C90760 C90766 C90768 C90780 15.6 1.018 7.0 19.8 1.020 7.0 16.0 1.020 7.0 2.8 1.057 6.0 12.6 1.023 7.5 46.0 1.006 7.0 4.3 1.040 6.5 9.2 1.027 7.0 17.4 1.021 7.0 43.5 1.007 7.0 MEAN S.D. N 18.7 14.79 10 1.024 .0151 10 6.9 .39 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C90791 C90797 C90802 C90807 C90816 C90818 C90820 C90831 C90836 C90840 47.3 1.004 7.0 42.5 1.006 6.5 9.7 1.018 7.0 13.0 1.023 7.0 31.7 1.012 7.0 10.5 1.026 6.5 14.0 1.018 7.0 21.5 1.016 7.0 46.5 1.007 7.0 14.0 1.024 7.0 MEAN S.D. N 25.1 15.47 10 1.015 .0079 10 6.9 .21 10 Covance 6329-212 3M T-6316.1 1547 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90843 C90851 C90852 C90856 C90863 C90877 C90880 C90888 C90892 C90897 13.1 1.022 6.5 16.7 1.015 7.0 25.0 1.012 7.0 14.2 1.018 7.0 6.4 1.039 7.0 11.8 1.019 7.0 33.8 1.012 6.5 8.5 1.027 6.5 3.2 1.043 6.5 24.8 1.015 7.0 MEAN S.D. N 15.8 9.53 10 1.022 .0109 10 6.8 .26 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C90905 C90907 C90917 C90921 C90934 C90947 C90953 C90957 C90960 C90968 11.7 1.021 7.5 15.3 1.018 7.0 18.3 1.016 7.0 47.0 1.003 7.0 9.2 1.026 6.5 4.0 1.058 6.5 46.8 1.006 6.5 26.6 1.012 6.5 13.8 1.024 6.5 6.5 1.037 6.5 MEAN S.D. N 19.9 15.56 10 1.022 .0160 10 6.8 .35 10 Covance 6329-212 3M T-6316.1 1548 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90716 10 - C90731 10 - C90738 30 - C90745 30 - C90746 30 - C90748 10 - C90760 100 - C90766 30 - C90768 10 - C90780 10 - + - + - - - ++ - + - - - + - - - - - - - - - - - - - - - - - - - +++ - + - + - - - Group: 2 Dose Level: 3 Dosage Unit: ppm C90791 0 - - - - - C90797 10 - - - - - C90802 10 - - - - - C90807 10 - + - - - C90816 10 - - - - - C90818 30 - + - - - C90820 10 - - - - - C90831 10 - - - - - C90836 10 - - - - - C90840 10 - - - - - Covance 6329-212 3M T-6316.1 1549 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90843 10 - + - - - C90851 10 - - - - - C90852 10 - - - - - C90856 10 - - - - - C90863 30 - + - - - C90877 10 - - - - - C90880 10 - - - - - C90888 30 - - - - - C90892 30 - ++ - - - C90897 10 - - - - - Group: 4 Dose Level: 100 Dosage Unit: ppm C90905 30 - - - - - C90907 10 - + - - - C90917 10 - + - - - C90921 0 - - - - - C90934 30 - + - - - C90947 30 - + - - - C90953 0 - - - - - C90957 10 - - - - - C90960 10 - + - - - C90968 30 - - - - - Covance 6329-212 3M T-6316.1 1550 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90716 0 0 1 1 0 4D BJ PQ C90731 0 0 1 1 0 1D BJ PQ C90738 0 0 0 1 0 4D BJ PQ C90745 0 0 1 1 0 4D CJ PQ C90746 0 0 1 1 0 4D BJ Q C90748 0 0 1 1 0 0 AJ Q C90760 4 0 1 3 0 4D DK P C90766 0 1 1 2 0 4D BJ PQ C90768 2 0 0 3 0 4D BJ PQ C90780 0 0 1 2 0 2D AJ Q Group: 2 Dose Level: 3 Dosage Unit: ppm C90791 0 0 1 1 0 0 AJ Q C90797 0 0 1 1 0 3D AJ Q C90802 0 0 1 1 0 4D BJ PQ C90807 0 0 1 1 0 4D BJ PQ C90816 0 0 1 2 0 4D BJ Q C90818 0 0 1 1 0 0 BJ Q C90820 0 0 1 2 0 4D BJ PQ C90831 0 0 1 1 0 4D BJ Q C90836 0 0 1 3 0 3D AJ Q C90840 0 0 1 2 0 4D BJ PQ Covance 6329-212 3M T-6316.1 1551 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90843 0 1 1 2 0 2B2D BJ Q C90851 0 0 1 1 0 2D BJ - C90852 0 0 1 1 0 4D BJ Q C90856 0 0 1 1 0 4D BJ Q C90863 0 1 1 3 0 1B3D BK Q C90877 0 0 0 1 0 2D BJ Q C90880 0 0 1 2 0 2D BJ Q C90888 0 1 1 1 0 1B3D CJ PQ C90892 0 0 1 2 0 4D CJ PQ C90897 0 0 1 2 0 0 BJ PQ Group: 4 Dose Level: 100 Dosage Unit: ppm C90905 0 0 1 2 0 2D BJ Q C90907 0 0 1 1 0 2D BJ Q C90917 1 0 0 1 0 1B BJ Q C90921 0 0 1 1 0 0 AJ Q C90934 0 1 1 1 0 0 BJ PQ C90947 0 0 1 2 0 4D CJ PQ C90953 0 0 0 1 0 0 AJ Q C90957 0 0 1 1 0 0 AJ Q C90960 1 0 1 1 0 3D BJ Q C90968 0 0 1 1 0 4D CJ PQ Covance 6329-212 3M T-6316.1 1552 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91122 C91129 C91148 C91155 C91160 C91174 C91176 C91178 C91181 C91182 27.9 1.003 7.0 11.5 1.014 6.5 16.8 1.009 6.5 15.9 1.010 6.5 49.8 1.002 6.5 7.8 1.025 6.5 5.6 1.025 6.5 10.5 1.018 7.0 28.2 1.007 7.0 15.8 1.010 7.0 MEAN S.D. N 19.0 13.21 10 1.012 .0082 10 6.7 .26 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C91192 C91196 C91204 C91215 C91221 C91223 C91228 C91233 C91241 C91248 2.4 1.031 6.5 11.4 1.013 7.0 26.5 1.008 6.5 21.8 1.007 7.0 45.6 1.002 7.0 47.0 1.002 6.5 9.0 1.020 7.0 2.5 1.026 6.5 33.5 1.006 6.5 22.3 1.006 7.0 MEAN S.D. N 22.2 16.26 10 1.012 .0102 10 6.8 .26 10 Covance 6329-212 3M T-6316.1 1553 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91262 C91274 C91281 C91284 C91286 C91288 C91293 C91299 C91304 C91305 23.0 1.004 7.0 7.3 1.020 6.5 49.0 1.002 6.0 15.7 1.010 7.0 7.2 1.016 7.0 1.3 1.038 6.0 7.0 1.020 6.5 47.0 1.002 7.0 17.3 1.011 6.5 17.1 1.015 6.5 MEAN S.D. N 19.2 16.50 10 1.014 .0109 10 6.6 .39 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C91326 C91327 C91329 C91332 C91337 C91348 C91355 C91370 C91374 C91379 5.6 1.027 6.5 46.0 1.002 6.5 18.6 1.013 6.5 20.7 1.007 6.5 11.8 1.018 6.5 47.8 1.002 7.0 40.0 1.006 6.5 8.2 1.022 6.5 25.5 1.009 6.5 7.8 1.018 6.5 MEAN S.D. N 23.2 16.13 10 1.012 .0086 10 6.6 .16 10 Covance 6329-212 3M T-6316.1 1554 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91122 0 - - - - - C91129 10 - - - - - C91148 0 - - - - - C91155 0 - - - - - C91160 0 - - - - - C91174 10 - - - - - C91176 10 - - - - - C91178 10 - - - - - C91181 0 - - - - - C91182 0 - - - - - Group: 2 Dose Level: 3 Dosage Unit: ppm C91192 10 - - - - - C91196 0 - - - - - C91204 0 - - - - - C91215 0 - - - - - C91221 0 - - - - - C91223 0 - - - - - C91228 10 - - - - - C91233 10 - - - - - C91241 0 - - - - - C91248 0 - - - - - Covance 6329-212 3M T-6316.1 1555 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91262 0 - - - - - C91274 10 - - - - - C91281 0 - - - - - C91284 0 - - - - - C91286 0 - - - - - C91288 10 - - - - - C91293 10 - - - - - C91299 0 - - - - - C91304 10 - - - - - C91305 0 - - - - - Group: 4 Dose Level: 100 Dosage Unit: ppm C91326 10 - - - - - C91327 0 - - - - - C91329 0 - - - - - C91332 0 - - - - - C91337 10 - - - - - C91348 0 - - - - - C91355 0 - - - - - C91370 10 - - - - - C91374 0 - - - - - C91379 10 - - - - - Covance 6329-212 3M T-6316.1 1556 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91122 0 0 0 1 0 1B1D AJ - C91129 0 1 1 2 0 2B BJ - C91148 0 0 1 1 0 0 BJ - C91155 0 0 1 1 0 0 AJ - C91160 0 0 1 2 0 0 AJ - C91174 0 1 1 1 0 4D BJ - C91176 0 1 0 3 0 4D BJ P C91178 0 0 0 1 0 4D BJ P C91181 0 0 1 2 0 2D AJ - C91182 0 0 1 1 0 4D AJ P Group: 2 Dose Level: 3 Dosage Unit: ppm C91192 0 0 0 1 0 4D BJ P C91196 0 0 1 2 0 2D BJ P C91204 0 0 0 2 0 0 AJ - C91215 0 0 1 1 0 1B BJ - C91221 0 0 0 1 0 1B AJ - C91223 0 0 0 1 0 0 AJ - C91228 0 0 0 1 0 1D BJ P C91233 0 0 1 1 0 0 BJ P C91241 0 0 1 1 0 1B AJ - C91248 0 0 1 1 0 0 AJ - Covance 6329-212 3M T-6316.1 1557 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91262 0 0 1 1 0 0 AJ - C91274 0 0 1 2 0 4D BJ P C91281 0 0 1 1 0 0 AJ - C91284 0 0 1 2 0 0 AJ P C91286 0 0 0 1 0 2D BJ - C91288 QS - - - - - - BJ - C91293 0 0 1 1 0 0 BJ P C91299 0 0 0 1 0 0 AJ - C91304 0 0 1 1 0 0 BJ - C91305 0 0 0 1 0 0 BJ - Group: 4 Dose Level: 100 Dosage Unit: ppm C91326 0 0 1 1 0 1B BJ - C91327 0 0 0 2 0 0 AJ - C91329 0 0 0 1 0 0 BJ - C91332 0 0 1 1 0 1B1D AJ - C91337 0 1 1 1 0 1B1D BJ P C91348 0 0 0 2 0 0 AJ - C91355 0 1 0 1 0 1B AJ - C91370 0 0 1 1 0 4D BJ P C91374 0 0 0 1 0 2B AJ - C91379 0 0 1 1 0 0 BJ P Covance 6329-212 3M T-6316.1 1558 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90721 C90735 C90736 C90738 C90739 C90747 C90751 C90752 C90755 C90766 30.5 1.012 6.5 11.5 1.021 7.0 7.2 1.036 7.0 10.7 1.026 7.0 7.4 1.033 6.5 14.7 1.021 7.0 15.4 1.020 7.0 23.7 1.016 7.5 24.9 1.017 7.5 8.7 1.037 7.0 MEAN S.D. N 15.5 8.17 10 1.024 .0087 10 7.0 .33 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C90795 C90799 C90802 C90803 C90805 C90806 C90824 C90825 C90839 C90840 24.4 1.011 7.5 16.7 1.017 7.5 10.5 1.025 7.0 20.5 1.021 7.5 14.5 1.029 7.5 10.1 1.024 7.0 8.3 1.031 7.0 7.9 1.031 7.0 15.6 1.019 7.5 8.5 1.043 6.5 MEAN S.D. N 13.7 5.65 10 1.025 .0090 10 7.2 .35 10 Covance 6329-212 3M T-6316.1 1559 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90847 C90855 C90858 C90859 C90864 C90865 C90871 C90872 C90882 C90886 38.5 1.011 7.5 12.0 1.031 6.5 4.0 1.052 7.0 6.7 1.032 7.0 10.0 1.022 7.0 38.5 1.009 7.0 5.0 1.042 6.5 6.0 1.043 7.5 5.8 1.034 7.0 10.1 1.028 7.0 MEAN S.D. N 13.7 13.33 10 1.030 .0137 10 7.0 .33 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C90916 C90931 C90937 C90940 C90944 C90945 C90954 C90956 C90957 C90963 47.5 a 1.009 7.0 5.0 1.037 7.5 27.5 1.011 7.0 6.8 1.037 7.0 21.7 1.016 7.0 10.5 1.027 6.5 8.2 1.029 6.5 6.3 1.028 7.0 9.8 1.031 7.5 11.2 1.028 7.0 MEAN S.D. N 15.4 13.36 10 1.025 .0100 10 7.0 .33 10 a Sample was overflowing at the time of collection. 1560 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90721 100 - - - ++ - C90735 30 - + - - - C90736 300 - + - - - C90738 300 - + - - - C90739 30 - - - - - C90747 30 - - - - - C90751 30 - - - - - C90752 30 - - - + - C90755 30 - + - - - C90766 300 - - - - - Group: 2 Dose Level: 3 Dosage Unit: ppm C90795 10 - - - - - C90799 30 - - - +++ - C90802 30 - - - ++ - C90803 100 - - - +++ - C90805 100 - + - + - C90806 30 - + - - - C90824 30 - + - - - C90825 100 - - - - - C90839 30 - + - - - C90840 >2000 - - - +++ - Covance 6329-212 3M T-6316.1 1561 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90847 10 - - - - - C90855 30 - + - - - C90858 100 - + - - - C90859 30 - + - - - C90864 30 - + - - - C90865 10 - - - - - C90871 100 - + - - - C90872 30 - - - - - C90882 30 - + - - - C90886 30 - + - - - Group: 4 Dose Level: 100 Dosage Unit: ppm C90916 10 - - - - - C90931 30 - +++ - - - C90937 30 - - - - - C90940 100 - ++ - - - C90944 30 - - - - - C90945 30 - + - - - C90954 30 - + - - - C90956 30 - + - - - C90957 30 - + - - - C90963 30 - + - - - Covance 6329-212 3M T-6316.1 1562 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90721 0 1 0 1 1G 1B BJ PQ C90735 0 0 1 2 0 4D BK - C90736 0 1 1 1 0 4D CK PQ C90738 0 0 1 2 0 4D BK PQ C90739 0 0 1 1 0 0 CK PQ C90747 0 0 1 2 0 4D BK PQ C90751 0 0 1 1 0 4D BK P C90752 0 0 1 1 0 3D BJ P C90755 0 0 1 1 0 3D BJ Q C90766 0 0 0 2 0 2B4D CK PQ Group: 2 Dose Level: 3 Dosage Unit: ppm C90795 0 0 1 1 0 3D BJ - C90799 1 0 0 1 0 3D BK Q C90802 0 0 1 2 0 4D BK PQ C90803 1 0 1 1 0 3D BK P C90805 0 0 0 2 0 2B4D BK PQ C90806 0 0 1 1 0 4D BK PQ C90824 0 1 1 2 0 4D BK Q C90825 0 0 1 2 0 4D BK PQ C90839 0 1 1 1 0 4D BK PQ C90840 4 0 1 2 0 4D DK P Covance 6329-212 3M T-6316.1 1563 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90847 0 0 1 1 0 0 BJ Q C90855 0 0 1 1 0 1D BK Q C90858 0 0 1 3 0 2B4D DKO PQT C90859 0 0 1 2 0 4D BK Q C90864 0 0 0 1 0 2D BK PQ C90865 0 0 1 1 0 0 BJ P C90871 4 1 1 2 0 1B1D DL - C90872 0 0 0 1 0 4D CK Q C90882 0 1 1 1 0 3D CK PQ C90886 0 1 1 1 0 3D BK Q Group: 4 Dose Level: 100 Dosage Unit: ppm C90916 0 0 0 1 0 0 BJ Q C90931 0 0 1 1 0 4D CK Q C90937 0 1 1 1 0 0 BJ P C90940 0 0 1 1 0 4D CK PQ C90944 0 0 1 2 0 4D BK PQ C90945 0 0 1 1 0 4D BK PQ C90954 0 0 1 2 0 4D BK PQ C90956 0 0 0 1 0 2D BK Q C90957 0 0 1 2 0 4D BK PQ C90963 1 4 0 2 0 0 BK PQ Covance 6329-212 3M T-6316.1 1564 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91127 C91128 C91133 C91137 C91144 C91153 C91154 C91158 C91161 C91183 2.2 1.044 6.5 11.2 1.018 6.5 8.8 1.018 7.0 47.1 a 1.003 6.5 2.0 1.042 7.0 11.0 1.017 7.0 16.0 1.016 7.0 11.0 1.023 7.0 49.4 a 1.005 7.5 22.1 1.008 7.0 MEAN S.D. N 18.1 16.96 10 1.019 .0140 10 6.9 .32 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C91196 C91207 C91210 C91211 C91214 C91221 C91224 C91235 C91243 C91244 12.6 1.010 6.5 3.3 1.027 6.5 4.0 1.032 6.5 6.2 1.021 7.0 6.4 1.024 7.5 10.6 1.018 7.5 11.1 1.012 6.5 50.6 a 1.001 6.5 35.0 1.006 7.0 19.6 1.009 7.0 MEAN S.D. N 15.9 15.38 10 1.016 .0100 10 6.8 .41 10 a Sample was overflowing at the time of collection. 1565 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91252 C91254 C91264 C91267 C91283 C91286 C91292 C91295 C91301 C91303 49.4 a 1.003 6.5 .8 1.072 6.0 8.7 1.016 7.0 8.5 1.009 7.0 11.4 1.016 6.5 14.0 1.011 7.0 8.0 1.018 6.5 5.5 1.017 6.5 10.5 1.017 7.0 13.1 1.019 7.0 MEAN S.D. N 13.0 13.35 10 1.020 .0190 10 6.7 .35 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C91312 C91323 C91328 C91339 C91340 C91351 C91354 C91357 C91369 C91371 8.5 1.018 6.5 12.1 1.018 6.0 6.5 1.026 6.5 7.8 1.023 7.0 5.7 1.033 7.5 27.8 1.008 6.5 5.8 1.026 6.5 23.3 1.008 7.0 14.1 1.017 6.5 20.5 1.013 7.5 MEAN S.D. N 13.2 8.01 10 1.019 .0081 10 6.8 .49 10 a Sample was overflowing at the time of collection. 1566 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91127 30 - - - - - C91128 10 - - - - - C91133 10 - - - - - C91137 10 - - - - - C91144 30 - - - - - C91153 10 - - - - - C91154 10 - - - - - C91158 10 - - - +++ - C91161 0 - - - + - C91183 0 - - - - - Group: 2 Dose Level: 3 Dosage Unit: ppm C91196 10 - - - - - C91207 30 - - - - - C91210 10 - - - - - C91211 10 - - - - - C91214 10 - - - + - C91221 10 - - - - - C91224 10 - - - - - C91235 0 - - - - - C91243 10 - - - - - C91244 10 - - - - - Covance 6329-212 3M T-6316.1 1567 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91252 10 - - - - - C91254 30 - - - - - C91264 10 - - - - - C91267 0 - - - - - C91283 10 - - - - - C91286 0 - - - - - C91292 10 - - - - - C91295 10 - - - - - C91301 10 - - - - - C91303 10 - - - - - Group: 4 Dose Level: 100 Dosage Unit: ppm C91312 10 - - - - - C91323 10 - - - - - C91328 30 - - - - - C91339 10 - - - - - C91340 10 - - - - - C91351 0 - - - - - C91354 10 - - - - - C91357 0 - - - - - C91369 10 - - - - - C91371 10 - - - - - Covance 6329-212 3M T-6316.1 1568 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91127 0 0 0 3 0 1D CJ P C91128 0 0 1 3 0 0 BJ P C91133 0 1 1 1 0 2D BJ P C91137 0 0 1 2 0 0 AJ - C91144 0 0 1 1 0 2B4D CK P C91153 0 0 1 2 0 4D BJ P C91154 0 0 1 1 0 2D BJ P C91158 4 1 1 2 0 4D BK - C91161 0 0 0 3 0 3B BK - C91183 0 0 1 1 0 1D BJ - Group: 2 Dose Level: 3 Dosage Unit: ppm C91196 0 0 1 1 0 0 BJ P C91207 0 1 1 2 0 0 CJ P C91210 0 0 1 2 0 4D BK P C91211 0 0 1 2 0 4D BK - C91214 1 0 0 1 0 4D BK - C91221 0 0 1 1 0 4D BJ P C91224 0 0 1 1 0 0 BJ P C91235 0 0 0 1 0 0 AJ - C91243 0 0 1 2 0 0 BJ P C91244 0 0 1 1 0 0 BJ - Covance 6329-212 3M T-6316.1 1569 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91252 0 0 0 1 0 0 AJ - C91254 0 0 1 1 0 3D DK P C91264 0 0 1 1 0 4D BJ P C91267 0 0 0 1 0 1B1D BJ - C91283 0 1 1 1 0 1D BJ - C91286 0 0 0 1 0 1D BJ - C91292 0 0 0 2 0 2B2D BK - C91295 0 1 1 1 0 0 BJ - C91301 0 0 1 1 0 0 BJ - C91303 0 1 1 1 0 2D BK P Group: 4 Dose Level: 100 Dosage Unit: ppm C91312 0 1 1 1 0 0 BJ P C91323 0 0 1 2 0 0 BJ - C91328 0 1 1 3 0 0 CK P C91339 0 0 1 1 0 4D BJ P C91340 0 1 1 1 0 4D BK P C91351 0 0 1 1 0 0 BJ - C91354 0 0 1 2 0 0 BJ - C91357 0 0 0 1 0 1D BJ - C91369 0 0 1 1 0 0 BJ - C91371 0 0 1 3 0 2B3D BJ P Covance 6329-212 3M T-6316.1 1570 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90712 C90723 C90727 C90730 C90755 C90758 C90759 C90764 C90766 C90774 7.5 1.027 7.0 48.5 a 1.010 6.5 11.2 1.030 7.0 17.7 1.020 6.5 49.0 a 1.011 7.0 16.3 1.020 6.5 11.0 1.026 7.0 7.6 1.035 6.5 15.0 1.026 7.0 9.3 1.038 7.0 MEAN S.D. N 19.3 15.90 10 1.024 .0092 10 6.8 .26 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C90781 C90792 C90793 C90798 C90799 C90800 C90812 C90822 C90829 C90832 11.5 1.027 6.5 13.0 1.026 7.0 7.8 1.037 7.0 15.5 1.024 7.0 11.1 1.027 7.0 16.2 1.020 7.5 4.5 1.050 7.0 8.2 1.040 6.5 25.3 1.018 7.0 23.7 1.017 7.5 MEAN S.D. N 13.7 6.72 10 1.029 .0106 10 7.0 .33 10 a Sample was overflowing at the time of collection. 1571 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90841 C90850 C90854 C90855 C90856 C90859 C90873 C90879 C90881 C90896 19.3 1.020 7.0 4.3 1.038 6.5 17.0 1.026 6.5 13.3 1.030 6.5 5.6 1.037 6.5 21.7 1.020 7.0 25.0 1.018 7.5 6.8 1.032 7.0 12.0 1.028 6.5 22.2 1.016 6.5 MEAN S.D. N 14.7 7.46 10 1.026 .0078 10 6.8 .35 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C90908 C90914 C90923 C90926 C90927 C90934 C90936 C90944 C90946 C90956 9.0 1.034 7.0 14.5 1.025 7.0 9.5 1.026 6.5 9.0 1.033 7.0 15.7 1.022 7.5 7.0 1.032 6.5 9.2 1.030 7.0 48.0 a 1.006 6.5 24.5 1.014 6.5 27.0 1.004 7.0 MEAN S.D. N 17.3 12.78 10 1.023 .0110 10 6.8 .34 10 a Sample was overflowing at the time of collection. 1572 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90712 >2000 - - - ++ - C90723 100 - - - - - C90727 >2000 - - - - - C90730 300 - - - - - C90755 100 - - - - - C90758 100 - - - - - C90759 30 - - - - - C90764 300 - - - - - C90766 300 - - - - - C90774 CO - - - +++ - Group: 2 Dose Level: 3 Dosage Unit: ppm C90781 100 - - - - - C90792 10 - - - - - C90793 >2000 - - - - - C90798 100 - - - - - C90799 30 - - - ++ - C90800 100 - - - +++ - C90812 300 - - - - - C90822 100 - - - - - C90829 30 - - - +++ - C90832 30 - - - - - Covance 6329-212 3M T-6316.1 1573 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90841 10 - - - + - C90850 30 - - - - - C90854 CO - - - +++ - C90855 100 - - - +++ - C90856 30 - - - - - C90859 30 - - - - - C90873 10 - - - - - C90879 300 - - - - - C90881 30 - - - + - C90896 100 - - - - - Group: 4 Dose Level: 100 Dosage Unit: ppm C90908 30 - - - - - C90914 30 - - - - - C90923 30 - - - - - C90926 30 - - - - - C90927 100 - - - - - C90934 30 - - - - - C90936 30 - - - - - C90944 0 - - - - - C90946 30 - - - - - C90956 10 - - - - - Covance 6329-212 3M T-6316.1 1574 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90712 1 1 1 2 0 2D CJ Q C90723 0 0 1 1 0 1B BJ PQ C90727 0 0 1 1 0 1B1D CJ P C90730 0 1 1 1 1G 0 BJ PQ C90755 0 0 1 3 0 0 BJ PQ C90758 0 1 1 1 1H 1B BK PQ C90759 0 0 1 1 0 1D CK - C90764 0 0 1 2 0 1B CK - C90766 0 1 0 2 1H 2B CK PQ C90774 4 0 0 0 0 1D GL - Group: 2 Dose Level: 3 Dosage Unit: ppm C90781 0 0 0 3 1G 4D CJ PQ C90792 0 0 1 1 0 2D CJ PQ C90793 0 0 1 1 0 4D CJ PQ C90798 1 1 1 1 0 3D CK P C90799 3 1 1 1 0 2D CJ PQ C90800 4 0 1 1 0 4D CK P C90812 0 0 1 2 0 4D DK Q C90822 0 1 1 1 0 0 CK PQ C90829 1 1 1 1 0 1B3D CK PQ C90832 0 0 1 1 0 4D BJ Q Covance 6329-212 3M T-6316.1 1575 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90841 1 0 1 2 0 3D BJ PQ C90850 2 0 1 1 0 0 CK PQ C90854 4 0 0 1 0 0 GL - C90855 4 0 1 0 0 0 CK Q C90856 0 1 1 2 0 2B2D CJ P C90859 1 1 1 1 0 1B1D CK PQ C90873 0 0 0 1 0 1B1D CJ - C90879 0 0 1 2 0 4D BJ PQ C90881 0 2 1 3 0 0 CJ PQ C90896 0 1 0 1 0 1B CK Q Group: 4 Dose Level: 100 Dosage Unit: ppm C90908 0 0 1 1 0 4D CJ PQ C90914 0 0 1 1 0 4D CJ P C90923 0 0 0 1 0 1D CJ Q C90926 0 0 0 2 0 3D CK PQ C90927 0 0 1 3 0 2B4D DK P C90934 0 0 0 1 0 0 CJ Q C90936 0 0 0 2 0 4D CK P C90944 1 1 1 1 0 2B AJ Q C90946 0 0 1 1 0 0 BK Q C90956 0 0 1 1 0 3D BJ Q Covance 6329-212 3M T-6316.1 1576 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91123 C91125 C91131 C91132 C91153 C91156 C91158 C91178 C91180 C91189 22.5 1.006 6.0 12.0 1.013 6.5 7.4 1.018 6.0 30.0 1.008 6.5 16.0 1.020 6.5 6.5 1.028 6.5 8.0 1.022 7.0 5.4 1.028 6.5 11.8 1.017 6.5 11.4 1.015 6.5 MEAN S.D. N 13.1 7.81 10 1.018 .0074 10 6.4 .28 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C91194 C91197 C91199 C91200 C91206 C91227 C91229 C91231 C91244 C91250 49.0 a 1.003 6.5 2.5 1.035 6.5 10.5 1.020 6.5 22.2 1.007 6.5 30.6 1.011 6.0 49.0 a 1.005 6.5 11.0 1.013 6.5 5.5 1.032 6.5 8.8 1.019 6.5 4.2 1.034 6.5 MEAN S.D. N 19.3 17.82 10 1.018 .0122 10 6.4 .16 10 a Sample was overflowing at the time of collection. 1577 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91255 C91258 C91264 C91269 C91270 C91274 C91289 C91294 C91297 C91305 16.2 1.016 6.0 9.5 1.017 6.5 49.0 a 1.004 6.0 13.0 1.021 6.5 7.5 1.033 6.0 12.5 1.011 6.5 5.0 1.030 6.5 9.0 1.018 6.5 7.5 1.021 6.5 7.6 1.037 6.5 MEAN S.D. N 13.7 12.84 10 1.021 .0101 10 6.4 .24 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C91314 C91319 C91326 C91334 C91346 C91356 C91359 C91363 C91372 C91373 18.7 1.011 6.5 34.6 1.008 6.5 6.7 1.029 6.5 36.5 1.009 6.5 7.2 1.026 6.0 3.6 1.037 6.5 7.5 1.024 6.5 3.1 1.043 6.5 39.0 1.004 6.5 12.7 1.022 6.5 MEAN S.D. N 17.0 14.38 10 1.021 .0131 10 6.4 .16 10 a Sample was overflowing at the time of collection. 1578 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91123 0 - - - - - C91125 0 - - - - - C91131 10 - - - - - C91132 10 - - - - - C91153 30 - - - + - C91156 100 - - - +++ - C91158 0 - - - - - C91178 10 - - - - - C91180 30 - - - - - C91189 0 - - - - - Group: 2 Dose Level: 3 Dosage Unit: ppm C91194 10 - - - + - C91197 30 - - - - - C91199 0 - - - - - C91200 0 - - - - - C91206 100 - - - - - C91227 0 - - - - - C91229 10 - - - - - C91231 30 - - - - - C91244 10 - - - - - C91250 10 - - - + - Covance 6329-212 3M T-6316.1 1579 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91255 0 - - - - - C91258 10 - - - - - C91264 0 - - - - - C91269 10 - - - - - C91270 0 - - - +++ - C91274 0 - - - - - C91289 0 - - - - - C91294 10 - - - - - C91297 10 - - - - - C91305 0 - - - - - Group: 4 Dose Level: 100 Dosage Unit: ppm C91314 0 - - - - - C91319 0 - - - - - C91326 30 - +++ - - - C91334 0 - - - - - C91346 10 - - - - - C91356 100 - - - - - C91359 10 - - - - - C91363 10 - - - - - C91372 10 - - - - - C91373 0 - - - - - Covance 6329-212 3M T-6316.1 1580 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91123 0 0 1 1 0 1B1D AJ P C91125 0 0 1 3 0 0 BJ P C91131 0 0 1 3 0 0 BJ P C91132 0 1 1 1 0 0 BJ - C91153 0 1 1 1 0 1B CK - C91156 4 2 1 0 0 0 DL - C91158 0 0 1 1 0 1D CK P C91178 1 0 1 1 0 0 CJ P C91180 0 1 1 1 0 0 BJ - C91189 0 1 1 1 0 1B BJ P Group: 2 Dose Level: 3 Dosage Unit: ppm C91194 0 0 1 3 0 0 AJ - C91197 0 0 0 1 0 1D CJ P C91199 0 0 0 2 0 1D CJ - C91200 0 0 1 1 0 1B AJ P C91206 0 0 1 1 1G 0 BK P C91227 0 0 1 1 0 0 AJ T C91229 0 0 1 1 0 0 BJ - C91231 0 0 1 1 0 1B CJ P C91244 0 0 1 1 0 0 BJ - C91250 2 0 1 1 0 0 CJ - Covance 6329-212 3M T-6316.1 1581 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91255 0 0 1 1 0 0 BJ P C91258 0 0 1 1 0 0 BJ - C91264 0 0 1 2 0 1B1D AJ - C91269 0 0 1 1 0 0 BJ - C91270 4 0 0 1 0 0 GL - C91274 0 0 1 1 0 0 BJ - C91289 0 1 1 1 0 3B CK P C91294 0 0 1 1 0 2D BJ P C91297 0 0 1 1 0 1B1D CK P C91305 0 1 1 1 0 0 CK P Group: 4 Dose Level: 100 Dosage Unit: ppm C91314 0 0 1 3 0 0 BJ P C91319 0 0 1 1 0 0 BJ C91326 0 0 1 2 0 2B CK P C91334 0 0 1 1 0 0 AJ P C91346 0 0 1 1 1G 0 CJ P C91356 0 0 0 1 0 0 CJ - C91359 0 0 0 1 0 0 CJ - C91363 0 0 1 1 0 3D CJ P C91372 0 0 1 0 1G 0 AJ - C91373 0 0 0 2 0 2B CJ - Covance 6329-212 3M T-6316.1 1582 Appendix 5 Individual Urine Chemistry Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90717 C90718 C90737 C90756 C90769 44 160 .33 1.20 36 131 .31 1.13 24 104 .34 1.49 19 140 .60 4.45 10 66 .25 1.66 MEAN S.D. N 27 13.5 5 120 36.4 5 .37 .135 5 1.99 1.394 5 Group: 5 Dose Level: 300 Dosage Unit: ppm C90971 C90972 C90988 a C90996 C91017 14 28 .31 .62 23 38 .62 1.03 6 18 - - 14 56 .40 1.60 43 63 .78 1.15 MEAN S.D. N 20 14.2 5 41 18.8 5 .53 .213 4 1.10 .403 4 a Unable to calculate excretion due to an inaccurate urine volume. Covance 6329-212 3M T-6316.1 1583 Appendix 5 Individual Urine Chemistry Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91126 C91140 a C91145 C91162 C91163 9 23 .23 .60 5 13 - - 10 30 .20 .60 21 70 .10 .35 33 128 .08 .29 MEAN S.D. N 16 11.4 5 53 47.3 5 .15 .074 4 .46 .164 4 Group: 5 Dose Level: 300 Dosage Unit: ppm C91393 C91394 C91404 C91415 C91438 a 23 37 .49 .79 26 40 .49 .76 31 62 .44 .87 11 56 .12 .60 8 16 - - MEAN S.D. N 20 9.9 5 42 18.0 5 .38 .178 4 .76 .113 4 a Unable to calculate excretion due to an inaccurate urine volume. Covance 6329-212 3M T-6316.1 1584 Appendix 5 Individual Urine Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90716 C90731 C90738 C90745 C90746 C90748 C90760 C90766 C90768 C90780 26 97 .41 1.51 23 132 .46 2.61 27 100 .43 1.60 55 358 .15 1.00 47 153 .59 1.93 4 26 .18 1.20 25 333 .11 1.43 39 189 .36 1.74 28 125 .49 2.18 7 41 .30 1.78 MEAN S.D. N 28 15.9 10 155 111.3 10 .35 .159 10 1.70 .469 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C90791 C90797 C90802 C90807 C90816 C90818 C90820 C90831 C90836 C90840 8 20 .38 .95 3 10 .13 .42 28 91 .27 .88 53 142 .69 1.85 24 50 .76 1.58 22 145 .23 1.52 22 100 .31 1.40 19 99 .41 2.13 7 30 .33 1.40 32 134 .45 1.88 MEAN S.D. N 22 14.5 10 82 51.3 10 .40 .197 10 1.40 .521 10 Covance 6329-212 3M T-6316.1 1585 Appendix 5 Individual Urine Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90843 C90851 C90852 C90856 C90863 C90877 C90880 C90888 C90892 C90897 11 107 .14 1.40 21 65 .35 1.09 11 68 .28 1.70 29 89 .41 1.26 64 324 .41 2.07 16 98 .19 1.16 10 50 .34 1.69 33 156 .28 1.33 82 238 .26 .76 20 78 .50 1.93 MEAN S.D. N 30 24.4 10 127 88.4 10 .32 .108 10 1.44 .405 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C90905 C90907 C90917 C90921 C90934 C90947 C90953 C90957 C90960 C90968 22 97 .26 1.13 22 100 .34 1.53 17 86 .31 1.57 4 16 .19 .75 26 137 .24 1.26 100 401 .40 1.60 9 20 .42 .94 13 50 .35 1.33 21 134 .29 1.85 52 236 .34 1.53 MEAN S.D. N 29 28.2 10 128 115.5 10 .31 .071 10 1.35 .335 10 Covance 6329-212 3M T-6316.1 1586 Appendix 5 Individual Urine Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91122 C91129 C91148 C91155 C91160 C91174 C91176 C91178 C91181 C91182 10 15 .28 .42 15 60 .17 .69 13 32 .22 .54 13 47 .21 .75 3 10 .15 .50 33 163 .26 1.27 41 106 .23 .59 31 95 .33 1.00 12 28 .34 .79 17 54 .27 .85 MEAN S.D. N 19 12.0 10 61 47.7 10 .25 .062 10 .74 .256 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C91192 C91196 C91204 C91215 C91221 C91223 C91228 C91233 C91241 C91248 56 210 .13 .50 23 59 .26 .67 16 35 .42 .93 12 31 .26 .68 12 9 .55 .41 3 9 .14 .42 30 101 .27 .91 38 187 .10 .47 5 29 .17 .97 11 29 .25 .65 MEAN S.D. N 21 16.5 10 70 73.0 10 .26 .139 10 .66 .214 10 Covance 6329-212 3M T-6316.1 1587 Appendix 5 Individual Urine Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91262 C91274 C91281 C91284 C91286 C91288 C91293 C91299 C91304 C91305 11 21 .25 .48 21 74 .15 .54 3 10 .15 .49 17 51 .27 .80 26 85 .19 .61 24 275 .03 .36 35 87 .24 .61 6 11 .28 .52 8 49 .14 .85 31 70 .53 1.20 MEAN S.D. N 18 11.0 10 73 76.5 10 .22 .132 10 .65 .244 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C91326 C91327 C91329 C91332 C91337 C91348 C91355 C91370 C91374 C91379 58 165 .32 .92 8 7 .37 .32 23 45 .43 .84 18 22 .37 .46 25 93 .30 1.10 9 9 .43 .43 6 22 .24 .88 29 110 .24 .90 12 34 .31 .87 14 59 .11 .46 MEAN S.D. N 20 15.4 10 57 51.3 10 .31 .098 10 .72 .271 10 Covance 6329-212 3M T-6316.1 1588 Appendix 5 Individual Urine Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90721 C90735 C90736 C90738 C90739 C90747 C90751 C90752 C90755 C90766 10 46 .30 1.40 21 79 .24 .91 57 179 .41 1.29 14 114 .15 1.22 28 141 .21 1.04 15 70 .22 1.03 28 78 .43 1.20 15 68 .36 1.61 38 72 .95 1.79 49 177 .43 1.54 MEAN S.D. N 28 15.9 10 102 47.8 10 .37 .227 10 1.30 .281 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C90795 C90799 C90802 C90803 C90805 C90806 C90824 C90825 C90839 C90840 20 56 .49 1.37 19 82 .32 1.37 43 86 .45 .90 41 70 .84 1.44 55 129 .80 1.87 25 101 .25 1.02 35 147 .29 1.22 33 141 .26 1.11 40 70 .62 1.09 29 138 .25 1.17 MEAN S.D. N 34 11.2 10 102 34.0 10 .46 .227 10 1.26 .274 10 Covance 6329-212 3M T-6316.1 1589 Appendix 5 Individual Urine Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90847 C90855 C90858 C90859 C90864 C90865 C90871 C90872 C90882 C90886 23 35 .89 1.35 34 133 .41 1.60 64 252 .26 1.01 51 124 .34 .83 33 76 .33 .76 12 32 .46 1.23 41 226 .20 1.13 73 211 .44 1.27 67 141 .39 .82 61 118 .62 1.19 MEAN S.D. N 46 20.5 10 135 76.2 10 .43 .197 10 1.12 .266 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C90916 a C90931 C90937 C90940 C90944 C90945 C90954 C90956 C90957 C90963 5 13 - - 67 189 .34 .94 18 61 .50 1.68 45 164 .31 1.12 21 81 .46 1.76 39 111 .41 1.17 28 117 .23 .96 61 109 .38 .69 86 192 .84 1.88 57 104 .64 1.16 MEAN S.D. N 43 25.3 10 114 56.2 10 .46 .186 9 1.26 .413 9 a Unable to calculate due to inaccurate urine volume. 1590 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Urine Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91127 C91128 C91133 C91137 a C91144 C91153 C91154 C91158 C91161 a C91183 46 198 .10 .44 22 101 .25 1.13 34 78 .30 .69 4 10 - - 19 148 .04 .30 12 88 .13 .97 19 60 .30 .96 52 108 .57 1.19 11 22 - - 9 35 .20 .77 MEAN S.D. N 23 16.1 10 85 58.0 10 .24 .164 8 .81 .318 8 Group: 2 Dose Level: 3 Dosage Unit: ppm C91196 C91207 C91210 C91211 C91214 C91221 C91224 C91235 a C91243 C91244 6 35 .08 .44 29 121 .10 .40 51 139 .20 .56 52 80 .32 .50 62 104 .40 .67 50 69 .53 .73 14 44 .16 .49 3 7 - - 9 26 .32 .91 10 40 .20 .78 MEAN S.D. N 29 22.9 10 66 43.7 10 .26 .148 9 .61 .173 9 a Unable to calculate due to inaccurate urine volume. 1591 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Urine Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91252 a 4 14 - - C91254 QS - - - - C91264 13 65 .11 .57 C91267 13 31 .11 .26 C91283 31 54 .35 .62 C91286 14 40 .20 .56 C91292 27 70 .22 .56 C91295 20 54 .11 .30 C91301 30 65 .32 .68 C91303 36 76 .47 1.00 MEAN S.D. N 21 10.7 9 52 20.2 9 .24 .133 8 .57 .229 8 Group: 4 Dose Level: 100 Dosage Unit: ppm C91312 C91323 C91328 C91339 C91340 C91351 C91354 C91357 C91369 C91371 16 71 .14 .60 17 81 .21 .98 15 88 .10 .57 37 98 .29 .76 77 166 .44 .95 11 36 .31 1.00 44 89 .26 .52 11 32 .26 .75 23 67 .32 .94 16 49 .33 1.00 MEAN S.D. N 27 20.8 10 78 38.4 10 .27 .098 10 .81 .191 10 a Unable to calculate due to inaccurate urine volume. 1592 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Urine Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C90712 C90723 a C90727 C90730 C90755 a C90758 C90759 C90764 C90766 C90774 28 145 .21 1.09 9 22 - - 25 137 .28 1.53 16 89 .28 1.58 16 25 - - 14 94 .23 1.53 41 126 .45 1.39 29 178 .22 1.35 21 141 .32 2.12 30 147 .28 1.37 MEAN S.D. N 23 9.5 10 110 52.5 10 .28 .077 8 1.50 .296 8 Group: 2 Dose Level: 3 Dosage Unit: ppm C90781 C90792 C90793 C90798 C90799 C90800 C90812 C90822 C90829 C90832 25 133 .29 1.53 26 141 .34 1.83 27 174 .21 1.36 20 117 .31 1.81 27 130 .30 1.44 29 85 .47 1.38 40 270 .18 1.22 37 205 .30 1.68 19 83 .48 2.10 18 76 .43 1.80 MEAN S.D. N 27 7.2 10 141 60.8 10 .33 .102 10 1.62 .273 10 a Sample was overflowing at the time of collection. 1593 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Urine Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C90841 C90850 C90854 C90855 C90856 C90859 C90873 C90879 C90881 C90896 27 79 .52 1.52 45 259 .19 1.11 17 90 .29 1.53 48 134 .64 1.78 23 215 .13 1.20 32 110 .69 2.39 27 96 .68 2.40 29 166 .20 1.13 19 138 .23 1.66 14 72 .31 1.60 MEAN S.D. N 28 11.2 10 136 61.5 10 .39 .221 10 1.63 .462 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C90908 C90914 C90923 C90926 C90927 C90934 C90936 C90944 a C90946 C90956 48 176 .43 1.58 32 136 .46 1.97 19 94 .18 .89 30 195 .27 1.76 39 103 .61 1.62 35 163 .24 1.14 39 162 .36 1.49 9 29 - - 14 48 .34 1.18 20 54 .54 1.46 MEAN S.D. N 28 12.5 10 116 58.9 10 .38 .142 9 1.45 .335 9 a Sample was overflowing at the time of collection. 1594 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Urine Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm C91123 C91125 C91131 C91132 C91153 C91156 C91158 C91178 C91180 C91189 14 34 .32 .76 12 56 .14 .67 12 86 .09 .64 9 33 .27 .99 43 93 .69 1.49 41 133 .27 .86 43 128 .34 1.02 26 154 .14 .83 57 78 .67 .92 32 84 .36 .96 MEAN S.D. N 29 16.8 10 88 40.9 10 .33 .206 10 .91 .240 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C91194 a C91197 C91199 C91200 C91206 C91227 a C91229 C91231 C91244 C91250 5 10 - - 76 205 .19 .51 35 100 .37 1.05 15 32 .33 .71 10 47 .31 1.44 9 23 - - 12 64 .13 .70 56 162 .31 .89 27 102 .24 .90 41 176 .17 .74 MEAN S.D. N 29 23.4 10 92 68.9 10 .26 .086 8 .87 .283 8 a Sample was overflowing at the time of collection. 1595 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Urine Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 3 Dose Level: 30 Dosage Unit: ppm C91255 C91258 C91264 a C91269 C91270 C91274 C91289 C91294 C91297 C91305 25 70 .40 1.13 11 76 .10 .72 6 16 - - 27 106 .35 1.38 26 150 .20 1.12 16 49 .20 .61 37 136 .18 .68 20 80 .18 .72 23 93 .17 .70 50 232 .38 1.76 MEAN S.D. N 24 12.6 10 101 60.4 10 .24 .107 9 .98 .395 9 Group: 4 Dose Level: 100 Dosage Unit: ppm C91314 C91319 C91326 C91334 C91346 C91356 C91359 C91363 C91372 C91373 13 49 .24 .92 12 38 .42 1.31 34 167 .23 1.12 13 42 .47 1.53 13 128 .09 .92 71 213 .26 .77 29 105 .22 .79 88 263 .27 .82 8 13 .31 .51 26 114 .33 1.45 MEAN S.D. N 31 27.4 10 113 82.0 10 .28 .107 10 1.01 .329 10 a Sample was overflowing at the time of collection. 1596 Covance 6329-212 3M T-6316.1 Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Author: Peter J. Thomford, PhD Study Completion Date: December 13, 2001 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-212 Sponsor Study Identification: 3M T-6316.1 Volume V of XII Page 1597 of 4873 Covance 6329-212 3M T-6316.1 VOLUME V OF XII CONTENTS Page APPENDIX 6............................................................................................................ 1599 Individual Anatomic Pathology Data ..................................................................... 1600 1598 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 1599 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90711 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/26/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 402.5 GRAMS DATE AND TIME OF NECROPSY: 07/26/99 8:11 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT EYE (EY) : NECROPSY: OBSERVED -MINERALIZATION, CORNEAL,-MINIMAL, >EXCRETION; FEW FECES; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE BRAIN (BR) : BRAIN (BR) : >APPEARANCE; HUNCHED; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE -DILATATION, VENTRICULAR,-PRESENT >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED LIVER (LI) : AT NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR,-MINIMAL, >EYES; SQUINTED; PERI-ORBITAL; VERIFIED MULTI-FOCAL AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >RESPIRATION; LABORED; VERIFIED AT -CAST, PROTEINACEOUS,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL -PYELONEPHRITIS,-MODERATE, MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, MULTI-FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 10 X 7 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL GRAY, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: SINGLE -EROSION,-MINIMAL, FOCAL BROWN ERODED AREA, 2 MM IN DIAMETER 1600 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90711 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/26/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 402.5 GRAMS DATE AND TIME OF NECROPSY: 07/26/99 8:11 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ URINARY BLADDER (UB) : URINARY BLADDER (UB) : -CALCULUS(I); WITHIN LUMEN: SINGLE >UNREMARKABLE CALCULUS, 10 X 6 X 6 MM, WHITE AND FIRM PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 1601 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90711 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/26/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 402.5 GRAMS DATE AND TIME OF NECROPSY: 07/26/99 8:11 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1602 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90712 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 397.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:25 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT EYE (EY) : NECROPSY: OBSERVED -HYPERPLASIA, CORNEAL EPITHELIUM,- >EXCRETION; FEW FECES; VERIFIED AT SLIGHT, FOCAL NECROPSY: NOT APPLICABLE -INFLAMMATION, ACUTE, CORNEAL,-MINIMAL, >APPEARANCE; THIN; VERIFIED AT FOCAL NECROPSY: OBSERVED -MINERALIZATION, CORNEAL,-MINIMAL, >APPEARANCE; HUNCHED; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : KIDNEY (KD) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -ROUGH SURFACE, DIFFUSE; BILATERAL -CYST,-MODERATE, MULTI-FOCAL NECROPSY: NOT APPLICABLE -DIFFUSELY LIGHT; BILATERAL: TAN -MINERALIZATION,-MODERATE, FOCAL >SKIN & PELAGE; PALE; ENTIRE BODY; -CYST(S); BILATERAL, CORTICAL SURFACE: -NEPHROPATHY, CHRONIC PROGRESSIVE,- VERIFIED AT NECROPSY: NOT APPLICABLE MULTIPLE CLEAR FLUID FILLED CYSTS, UP SEVERE, DIFFUSE TO 2 MM IN DIAMETER -THROMBUS,-MODERATELY SEVERE, FOCAL -LARGE; BILATERAL: EACH IS UP TO 28 X 20 X 13 MM PANCREAS (PA) : PANCREAS (PA) : -LARGE VESSELS; DIFFUSE, UP TO 3 MM IN -HEMORRHAGE,-MODERATE, FOCAL DIAMETER (CAP 23) -INFLAMMATION, VASCULAR,-MODERATELY SEVERE, FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -DARK FOCUS(I)/AREA(S); RIGHT: SINGLE -ANGIECTASIS,-MODERATELY SEVERE, FOCAL DARK BROWN FOCUS, 1 MM IN DIAMETER -HEMORRHAGE,-MODERATELY SEVERE, FOCAL -HYPERPLASIA, CORTICAL,-SLIGHT, FOCAL -MINERALIZATION,-MINIMAL, FOCAL -THROMBUS,-MODERATELY SEVERE, FOCAL 1603 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90712 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 397.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:25 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: RIGHT IS 20 X 11 X -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE 10 MM, LEFT IS 18 X 11 X 10 MM; BOTH -INFLAMMATION, CHRONIC,-MODERATE, MULTI- APPEAR SOFT FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PARATHYROID (PT) : PARATHYROID (PT) : -LARGE; BILATERAL: 2 MM IN DIAMETER -HYPERPLASIA,-MODERATE, DIFFUSE PITUITARY (PI) : -HYPERPLASIA,-MODERATE, FOCAL STOMACH, GL (ST) : -MINERALIZATION,-SLIGHT, MULTI-FOCAL PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, URETHRA: GREEN AND FIRM MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 5 X 1 >UNREMARKABLE MM EPIDIDYMIDES (EP) : EPIDIDYMIDES (EP) : -SMALL; BILATERAL: EACH IS 40 X 10 X 4 -HYPOSPERMIA,-PRESENT MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT MAMMARY, MALE (MM) : -PIGMENT,-MINIMAL, MULTI-FOCAL 1604 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90712 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 397.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:25 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ AORTA (AO) : AORTA (AO) : -LARGE; DIFFUSE: UP TO 4 MM IN >UNREMARKABLE DIAMETER (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY MATERIAL -MALOCCLUSION; BILATERAL: LOWER INCISORS -ANIMAL THIN -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN OFF AT BASE -------------------------------------------- 1605 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90712 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 397.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:25 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1606 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90713 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/10/99 STUDY DAY OF DEATH: 684 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 533.2 GRAMS DATE AND TIME OF NECROPSY: 12/10/99 8:11 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; AXILLARY EYE (EY) : EYE (EY) : REGION-RIGHT; VERIFIED AT NECROPSY: -WHITE INTRAOCULAR MATERIAL >UNREMARKABLE NOT OBSERVED BRAIN (BR) : BRAIN (BR) : >APPEARANCE; THIN; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >EYES; PALE; EYE-RIGHT; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -RED FOCUS(I)/AREA(S); ALL LOBES: >UNREMARKABLE >SKIN & PELAGE; BROWN HAIR COAT; MULTIPLE DARK RED AREAS, 2 MM IN PERINEAL AREA; VERIFIED AT NECROPSY: DIAMETER; COLLECTED ON ROUTINE NOT OBSERVED SECTIONS >RESPIRATION; LABORED; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED MODERATELY SEVERE, MULTI-FOCAL AT NECROPSY: NOT APPLICABLE ADRENAL, MEDULLA (MA) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALONG MARGIN -INFILTRATE, MACROPHAGE, ALVEOLAR,- OF RIGHT CAUDAL LOBE: SINGLE TAN, MINIMAL, MULTI-FOCAL SLIGHTLY RAISED AREA, 1 MM IN DIAMETER -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT 1607 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90713 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/10/99 STUDY DAY OF DEATH: 684 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 533.2 GRAMS DATE AND TIME OF NECROPSY: 12/10/99 8:11 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 10 X 6 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, DARK BROWN MATERIAL -------------------------------------------- 1608 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90713 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/10/99 STUDY DAY OF DEATH: 684 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 533.2 GRAMS DATE AND TIME OF NECROPSY: 12/10/99 8:11 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1609 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90714 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 639.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:33 PROSECTOR: KIMBERLY DURLAND RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0321 .0050 % .0140 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0269 .0042 % .0117 WEIGHT TAKEN BRAIN (BR) 2.2920 .3586 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.2054 .3450 % .9622 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.2469 .3515 % .9803 WEIGHT TAKEN LIVER (LI) 17.9422 2.8070 % 7.8282 WEIGHT TAKEN LUNG (LU) 2.1290 .3331 % .9289 WEIGHT TAKEN SPLEEN (SP) .8259 .1292 % .3603 WEIGHT TAKEN LEFT TESTIS (TE0) 1.5323 .2397 % .6685 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.5470 .2420 % .6750 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0108 .0017 % .0047 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0136 .0021 % .0059 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1610 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90714 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 639.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:33 PROSECTOR: KIMBERLY DURLAND RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1611 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90714 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 639.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:33 PROSECTOR: KIMBERLY DURLAND RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1612 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90715 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/05/99 STUDY DAY OF DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 617.9 GRAMS DATE AND TIME OF NECROPSY: 11/05/99 14:15 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; NO FECES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, NECROPSY: NOT OBSERVED EOSINOPHILIC,-SLIGHT, FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -HYPERPLASIA, BILE DUCT,-SLIGHT, MULTI- NECROPSY: NOT APPLICABLE FOCAL >SKIN & PELAGE; BROWN HAIR COAT; -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- VENTRAL-ABDOMINAL; VERIFIED AT FOCAL NECROPSY: NOT OBSERVED KIDNEY (KD) : >SKIN & PELAGE; BROWN HAIR COAT; -CAST, PROTEINACEOUS,-SLIGHT, MULTI- PERINEAL AREA; VERIFIED AT NECROPSY: FOCAL OBSERVED PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL 1613 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90715 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/05/99 STUDY DAY OF DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 617.9 GRAMS DATE AND TIME OF NECROPSY: 11/05/99 14:15 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 10 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- TAN AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: MOIST, BROWN MATERIAL -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER AND LOWER INCISORS -------------------------------------------- 1614 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90715 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/05/99 STUDY DAY OF DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 617.9 GRAMS DATE AND TIME OF NECROPSY: 11/05/99 14:15 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1615 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90716 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 896.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:15 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; HEAD- LIVER (LI) : LIVER (LI) : ENTIRE; VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -FOCUS, ALTERED HEPATOCELLULAR, >WART LIKE LESION(S); DORSAL; WEEK 105; MULTIPLE DARK RED AREAS, UP TO 4 X 3 EOSINOPHILIC,-MODERATE, MULTI-FOCAL VERIFIED AT NECROPSY: OBSERVED MM; COLLECTED ON ROUTINE SECTIONS -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- SLIGHT, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL -VACUOLATION,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 1616 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90716 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 896.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:15 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : SKIN (SK) : -RAISED AREA(S); DORSAL LUMBAR REGION: -HEMORRHAGE,-MINIMAL, MULTI-FOCAL SINGLE FIRM, RED AREA, 17 X 15 X 12 MM -B-FIBROMA,-NEOPLASM PRESENT (CAP 22) PROSTATE (PR) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD, HAIRCOAT: DRY RED MATERIAL -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 1617 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90716 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 896.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:15 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1618 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 20 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90717 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 462.2 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:59 PROSECTOR: DONIA MEYER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0353 .0076 % .0163 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0295 .0064 % .0136 WEIGHT TAKEN BRAIN (BR) 2.1647 .4683 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.6555 .3582 % .7648 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.5543 .3363 % .7180 WEIGHT TAKEN LIVER (LI) 12.1561 2.6301 % 5.6156 WEIGHT TAKEN LUNG (LU) 1.4254 .3084 % .6585 WEIGHT TAKEN SPLEEN (SP) .7066 .1529 % .3264 WEIGHT TAKEN LEFT TESTIS (TE0) 1.6716 .3617 % .7722 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.7505 .3787 % .8087 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0146 .0032 % .0067 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0107 .0023 % .0049 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 1619 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 21 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90717 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 462.2 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:59 PROSECTOR: DONIA MEYER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), DUODENUM (DU) ------------------------------------------------------------------------------------------------------------------------------------ 1620 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 22 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90718 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 449.7 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:41 PROSECTOR: GREGORY CODDINGTON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0344 .0076 % .0159 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0306 .0068 % .0141 WEIGHT TAKEN BRAIN (BR) 2.1686 .4822 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.3251 .2947 % .6110 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.3182 .2931 % .6079 WEIGHT TAKEN LIVER (LI) 10.8628 2.4156 % 5.0091 WEIGHT TAKEN LUNG (LU) 1.4621 .3251 % .6742 WEIGHT TAKEN SPLEEN (SP) .8431 .1875 % .3888 WEIGHT TAKEN LEFT TESTIS (TE0) 1.4436 .3210 % .6657 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.4531 .3231 % .6701 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0142 .0032 % .0065 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0169 .0038 % .0078 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 1621 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 23 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90718 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 449.7 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:41 PROSECTOR: GREGORY CODDINGTON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), THYMUS (TH), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), EPIDIDYMIDES (EP) ------------------------------------------------------------------------------------------------------------------------------------ 1622 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 24 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90719 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 307.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:19 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 8.9709 2.9193 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1623 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 25 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90719 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 307.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:19 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 1624 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 26 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90720 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/05/99 9:58 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATELY SEVERE, DIFFUSE PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MODERATE, MULTI- FOCAL -FIBROSIS,-MODERATE, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 5 X 4 MM, TAN, RED, -B-ADENOMA,-NEOPLASM PRESENT SOFT, AND LOBULATED JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL 1625 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90720 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/05/99 9:58 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1626 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 28 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90721 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/26/98 STUDY DAY OF DEATH: 305 STUDY WEEK OF DEATH: 44 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/26/98 12:55 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-THORACIC; WEEK 42; LIVER (LI) : LIVER (LI) : 30 X 30MM; VERIFIED AT NECROPSY: NOT -LARGE; ALL LOBES -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO OBSERVED -MOTTLED; ALL LOBES: DARK RED, BROWN NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT >SKIN & PELAGE; BROWN HAIR COAT; AND TAN PERINEAL AREA; VERIFIED AT NECROPSY: KIDNEY (KD) : NOT OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EXCRETION; FEW FECES; VERIFIED AT MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO >SKIN & PELAGE; PALE; ENTIRE BODY; NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE SPLEEN (SP) : SPLEEN (SP) : -LARGE; 70 X 12 X 10 MM -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PANCREAS (PA) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -MASS(ES); LEFT, INVOLVING ENTIRE -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO GLAND: SINGLE MASS, 20 MM IN DIAMETER; NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT DARK RED, GRAY, TAN AND SOFT; SAME ON CUT SURFACE (CAP 24); REGIONAL LN (SEE RENAL) ADRENAL, MEDULLA (MA) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 1627 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 29 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90721 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/26/98 STUDY DAY OF DEATH: 305 STUDY WEEK OF DEATH: 44 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/26/98 12:55 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -LARGE; UP TO 25 MM IN DIAMETER -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO -MOTTLED; DARK RED, GRAY AND TAN NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MARROW, FEMUR (FM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MARROW, STERNUM (SE) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYMUS (TH) : THYMUS (TH) : -MASS(ES); INVOLVING ENTIRE GLAND: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO SINGLE MASS, 20 X 15 X 10 MM, DARK RED NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT TAN AND FIRM; SAME ON CUT SURFACE; REGIONAL LN (SEE MEDIASTINAL) LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MUSCLE, SKELETAL (SM) : MUSCLE, SKELETAL (SM) : -MASS(ES); EXTENDING FROM LEFT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO MANDIBLE TO LEFT DORSAL ASPECT OF NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT SKULL CAP: SINGLE MASS, 20 X 15 X 10 MM; TAN AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE MANDIBULAR) PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 1628 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 30 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90721 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/26/98 STUDY DAY OF DEATH: 305 STUDY WEEK OF DEATH: 44 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/26/98 12:55 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SALIV GL, MANDIB (SG) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -MALIGNANT LYMPHOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL, INCLUDING DEEP -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO CERVICAL LN'S: UP TO 12 X 10 X 8 MM; NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT COLLECTED LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -LARGE; UP TO 6 MM IN DIAMETER -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO -DIFFUSELY RED; DARK RED (CAP 22) NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT MESENTERY (MY) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI- FOCAL LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -LARGE; UP TO 8 MM IN DIAMETER (CAP -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO 23) NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -MOTTLED; DARK RED AND TAN LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: EACH IS UP TO 25 X -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO 15 X 10 MM (CAP 26) NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 1629 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 31 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90721 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/26/98 STUDY DAY OF DEATH: 305 STUDY WEEK OF DEATH: 44 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/26/98 12:55 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL: DARK RED AND TAN LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; BILATERAL: EACH IS UP TO 12 X -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO 10 X 10 MM (CAP 25) NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW -FLUID-THORACIC CAVITY; FILLED WITH A DARK RED FLUID -------------------------------------------- 1630 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 32 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90721 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/26/98 STUDY DAY OF DEATH: 305 STUDY WEEK OF DEATH: 44 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/26/98 12:55 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) ------------------------------------------------------------------------------------------------------------------------------------ 1631 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 33 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90722 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 859.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:29 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER, OIL RED O (LI1) : VERIFIED AT NECROPSY: OBSERVED -POSITIVE OIL RED O STAINING,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL PANCREAS (PA) : PANCREAS (PA) : -LARGE VESSELS; VESSELS NEAR DUODENUM: -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL UP TO 2 MM IN DIAMETER (CAP 22) -HYPERTROPHY, VASCULAR,-MODERATELY SEVERE, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 X 5 X 5 MM (RIGHT IS 4 -ANGIECTASIS,-MODERATELY SEVERE, FOCAL X 3 X 2 MM) -DEGENERATION, CYSTIC, CORTICAL,- MINIMAL, FOCAL -HEMORRHAGE,-MINIMAL, FOCAL -THROMBUS,-SLIGHT, FOCAL HEART (HT) : -FIBROSIS,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -LIGHT FOCUS(I)/AREA(S); MID LEFT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL ASPECT: SINGLE DARK RED FOCUS, 1 MM IN -B-ADENOMA,-NEOPLASM PRESENT DIAMETER 1632 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 34 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90722 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 859.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:29 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -EDEMA,-SLIGHT, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1633 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 35 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90722 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 859.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:29 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1634 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 36 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90723 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/22/99 STUDY DAY OF DEATH: 421 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 781.3 GRAMS DATE AND TIME OF NECROPSY: 03/22/99 14:50 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; PROTRUDING PENIS; VERIFIED LIVER (LI) : AT NECROPSY: OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL, FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL RECTUM (RE) : -PARASITISM,-PRESENT CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 21) -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PENIS (PE) : PENIS (PE) : -PROTRUDING; ENTIRE LENGTH: DRY, RED, -EDEMA,-SEVERE, FOCAL AND BROWN; COLLECTED -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFLAMMATION, CHRONIC ACTIVE,-SLIGHT, MULTI-FOCAL -PROLAPSE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 1635 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 37 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90723 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/22/99 STUDY DAY OF DEATH: 421 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 781.3 GRAMS DATE AND TIME OF NECROPSY: 03/22/99 14:50 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1636 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 38 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90724 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 928.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:41 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- MULTIPLE DARK RED AREAS, UP TO 2 X 1 FOCAL MM; COLLECTED ON ROUTINE SECTIONS -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL, MULTI-FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -DILATATION, SINUSOIDAL,-MODERATE, FOCAL 1637 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 39 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90724 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 928.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:41 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 9 X 7 X 5 MM, -B-ADENOMA,-NEOPLASM PRESENT RED, DARK RED, TAN, GRAY AND SOFT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL (CAP 23) LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -DIFFUSELY RED; DARK RED (CAP 22) -HEMORRHAGE,-SLIGHT, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); RIGHT -EROSION,-MODERATE, FOCAL HINDFOOT: SINGLE DARK BROWN AND MOIST -FIBROSIS,-MODERATE, FOCAL ABRADED AREA, 5 MM IN DIAMETER; -INFLAMMATION, ACUTE,-MODERATE, FOCAL COLLECTED DIAPHRAGM (DP) : DIAPHRAGM (DP) : -MASS(ES); ATTACHED TO DIAPHRAGM, NEAR -B-FIBROMA,-NEOPLASM PRESENT RIGHT MEDIAN LOBE OF LIVER: SINGLE MASS, 40 X 38 X 15 MM, RED, TAN, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE MEDIASTINAL) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1638 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 40 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90724 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 928.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:41 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1639 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 41 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90725 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 346.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:50 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 10.6740 3.0770 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1640 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 42 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90725 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 346.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:50 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 1641 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 43 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90726 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 602.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:21 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LIVER (LI) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, MULTI-FOCAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1642 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 44 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90726 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 602.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:21 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; RIGHT: 15 X 10 X 8 MM (LEFT IS -DILATATION, SINUSOIDAL,-MODERATELY 9 X 4 X 2 MM) (CAP 22) SEVERE, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); RIGHT -EDEMA,-MODERATE, MULTI-FOCAL HINDFOOT: SINGLE DARK RED AND TAN -EROSION,-MODERATELY SEVERE, MULTI- ABRADED AREA, 13 X 12 MM; COLLECTED FOCAL -EXOSTOSES,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1643 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 45 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90726 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 602.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:21 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1644 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 46 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90727 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 486.9 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:49 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); PAW-FRONT-LEFT; LIVER (LI) : LIVER (LI) : WEEK 95; VERIFIED AT NECROPSY: -RED FOCUS(I)/AREA(S); ALL LOBES: -DEGENERATION, CYSTIC,-MODERATE, MULTI- OBSERVED MULTIPLE DARK RED AREAS, UP TO 5 MM IN FOCAL >WART LIKE LESION(S); PAW-FRONT-RIGHT; DIAMETER; COLLECTED ON ROUTINE -VACUOLATION, HEPATOCELLULAR,-MINIMAL, WEEK 95; VERIFIED AT NECROPSY: SECTIONS MULTI-FOCAL OBSERVED KIDNEY (KD) : KIDNEY (KD) : >APPEARANCE; THIN; VERIFIED AT -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT NECROPSY: OBSERVED -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EYES; RED DISCHARGE; EYE-RIGHT; -DIFFUSELY LIGHT; BILATERAL: TAN SEVERE, DIFFUSE VERIFIED AT NECROPSY: OBSERVED -CYST(S); BILATERAL, DIFFUSE: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -MASS(ES); RIGHT: SINGLE MASS, 35 X 30 -ATROPHY, CORTICAL,-MODERATELY SEVERE, X 25 MM, RED, DARK RED, TAN, SOFT AND DIFFUSE LOBULATED; CUT SURFACE CONTAINS A TAN -HEMORRHAGE,-SLIGHT, MULTI-FOCAL CASEOUS SEMISOLID; COLLECTED; REGIONAL -MINERALIZATION,-MODERATELY SEVERE, LN (SEE RENAL) MULTI-FOCAL -NECROSIS, CORTICAL,-MODERATELY SEVERE, MULTI-FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -VACUOLATION,-MODERATE, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE 1645 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 47 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90727 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 486.9 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:49 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 20 X 15 X 6 -MINERALIZATION, VASCULAR,-MINIMAL, MM; BOTH ARE SOFT MULTI-FOCAL BONE, FEMUR (FE) : -OSTEODYSTROPHY, FIBROUS,-SLIGHT, MULTI- FOCAL LUNG (LU) : LUNG (LU) : -MASS(ES); ALL LOBES: MULTIPLE MASSES, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL UP TO 7 X 5 X 5 MM, TAN, BROWN AND -INFILTRATE, MACROPHAGE, ALVEOLAR,- FIRM; SAME ON CUT SURFACE; COLLECTED MODERATE, MULTI-FOCAL INTACT; REGIONAL LN (TRACHEOBRONCHIAL) -N-ADRENAL CORTICAL CARCINOMA,-NEOPLASM NORMAL (CAP 22) PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL -THROMBUS,-SLIGHT, FOCAL PARATHYROID (PT) : -HYPERPLASIA,-MODERATE, DIFFUSE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -MINERALIZATION,-MODERATE, MULTI-FOCAL MULTIPLE BROWN AREAS, UP TO 1 MM IN DIAMETER -THICKENED WALL; DIFFUSE 1646 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 48 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90727 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 486.9 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:49 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 7 X 3 >UNREMARKABLE MM CAUSE OF DEATH (CD) : -ADRENAL CORTICAL CARCINOMA,-PRESENT LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; LEFT: 10 X 10 X 5 MM (RIGHT IS -DILATATION, SINUSOIDAL,-SLIGHT, MULTI- 5 X 3 X 3 MM) (CAP 23) FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); BILATERAL FRONT >UNREMARKABLE FEET: SINGLE DARK RED, TAN AND RAISED AREAS ON EACH, LEFT IS 20 X 5 X 5 MM, RIGHT IS 10 X 3 X 3 MM; BOTH COLLECTED AORTA (AO) : AORTA (AO) : -LARGE; DIFFUSE: UP TO 9 MM IN -MINERALIZATION,-MODERATELY SEVERE, DIAMETER; COLLECTED MULTI-FOCAL AORTIC ARCH (AA) : AORTIC ARCH (AA) : -LARGE; DIFFUSE: UP TO 10 MM IN -MINERALIZATION,-MODERATELY SEVERE, DIAMETER; COLLECTED INTACT WITH HEART MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED AND DRY -ANIMAL THIN -------------------------------------------- 1647 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 49 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90727 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 486.9 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:49 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), PENIS (PE), AUDITORY SEB GL (AS), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, TRACHEOBRON (TB) ------------------------------------------------------------------------------------------------------------------------------------ 1648 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 50 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90728 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 890.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:16 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -MASS(ES); ALONG MARGIN OF LEFT MEDIAN -TELANGIECTASIS,-MODERATE, MULTI-FOCAL LOBE: SINGLE MASS, 13 X 12 X 10 MM, -VACUOLATION, HEPATOCELLULAR,- RED, DARK RED, TAN AND SOFT; SAME ON MODERATELY SEVERE, MULTI-FOCAL CUT SURFACE; COLLECTED; REGIONAL LN -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM (HEPATIC) NOT IDENTIFIED PRESENT -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 5 X 4 MM; SOME ARE SUNKEN WITH A STELLATE PATTERN; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL THYMUS (TH) : -CYST,-PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -HYPERPLASIA, C-CELL,-SLIGHT, MULTI- FOCAL PARATHYROID (PT) : -HYPERPLASIA,-MODERATE, FOCAL PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); LEFT ANTERIOR -HEMORRHAGE,-MODERATE, MULTI-FOCAL ASPECT: SINGLE DARK BROWN FOCUS, 2 MM -B-ADENOMA,-NEOPLASM PRESENT IN DIAMETER 1649 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 51 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90728 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 890.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:16 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- URETHRA: GREEN AND FIRM FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 1650 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 52 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90728 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 890.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:16 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER, OIL RED O (LI1), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1651 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90729 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/01/99 10:37 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : VERIFIED AT NECROPSY: NOT OBSERVED -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI- FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT TESTIS (TE) : TESTIS (TE) : -SMALL; LEFT: 20 X 9 X 5 MM (RIGHT, -HEMORRHAGE,-SEVERE, MULTI-FOCAL SEE LARGE COMMENT); APPEARS SOFT -MINERALIZATION,-MINIMAL, FOCAL -LARGE; RIGHT: 28 X 17 X 12 MM -B-INTERSTITIAL CELL TUMOR,-NEOPLASM -RED/YELLOW FOCUS(I)/AREA(S); RIGHT, PRESENT BENEATH TUNICA: MULTIPLE DARK RED AND YELLOW INDISTINCT AREAS LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI- FOCAL -FIBROSIS,-MODERATE, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 1652 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 54 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90729 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/01/99 10:37 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 5 X 3 MM, DARK RED AND -B-ADENOMA,-NEOPLASM PRESENT SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EROSION,-MODERATE, MULTI-FOCAL MULTIPLE BLACK AREAS, UP TO 2 MM IN DIAMETER JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; PERINEUM: MOIST, YELLOW MATERIAL -STAINS - SKIN/HAIRCOAT; INVOLVING ENTIRE HEAD: RED, MOIST MATERIAL -------------------------------------------- 1653 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 55 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90729 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/01/99 10:37 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1654 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 56 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90730 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/20/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 494.3 GRAMS DATE AND TIME OF NECROPSY: 05/20/99 7:49 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; HUNCHED; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >APPEARANCE; HEAD TILT; RIGHT; VERIFIED -MASS(ES); VISCERAL SURFACE OF LEFT -HYPERPLASIA, BILE DUCT,-SLIGHT, MULTI- AT NECROPSY: NOT APPLICABLE LATERAL LOBE: TWO MASSES, ONE IS 20 X FOCAL >SKIN & PELAGE; ROUGH HAIR COAT; 15 X 5 MM, THE OTHER IS 8 X 5 X 3 MM; -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- VERIFIED AT NECROPSY: NOT APPLICABLE THE LARGER MASS IS DARK BROWN, RED, FOCAL AND SOFT; SAME ON CUT SURFACE; THE -VACUOLATION, HEPATOCELLULAR,-SLIGHT, SMALLER MASS IS RED AND SOFT; SAME ON MULTI-FOCAL CUT SURFACE; BOTH MASSES COLLECTED; -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM REGIONAL LN (HEPATIC) NOT IDENTIFIED PRESENT KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY DARK; GREEN -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL 1655 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 57 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90730 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/20/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 494.3 GRAMS DATE AND TIME OF NECROPSY: 05/20/99 7:49 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 12 X 8 MM, RED, DARK -HEMORRHAGE,-MODERATELY SEVERE, MULTI- RED, TAN, GRAY, AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, URETHRA: GREEN AND FIRM MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 1656 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 58 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90730 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/20/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 494.3 GRAMS DATE AND TIME OF NECROPSY: 05/20/99 7:49 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1657 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 59 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90731 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 591.1 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:53 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0444 .0075 % .0199 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0410 .0069 % .0184 WEIGHT TAKEN BRAIN (BR) 2.2260 .3766 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.9548 .3307 % .8782 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.0881 .3533 % .9381 WEIGHT TAKEN LIVER (LI) 18.2920 3.0946 % 8.2174 WEIGHT TAKEN LUNG (LU) 2.0427 .3456 % .9177 WEIGHT TAKEN SPLEEN (SP) .7911 .1338 % .3554 WEIGHT TAKEN LEFT TESTIS (TE0) 1.7843 .3019 % .8016 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.8109 .3064 % .8135 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0107 .0018 % .0048 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0156 .0026 % .0070 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -MOTTLED; ALL LOBES: RED AND TAN >UNREMARKABLE KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL PANCREAS (PA) : -FIBROSIS,-MINIMAL, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL THYMUS (TH) : THYMUS (TH) : -MOTTLED; RIGHT LOBE: DARK RED AND TAN >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN 1658 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 60 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90731 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 591.1 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:53 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 1659 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 61 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90732 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 400.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:43 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 13.3343 3.3261 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1660 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 62 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90732 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 400.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:43 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 1661 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 63 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90733 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 10:37 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); TAIL; WEEK 82; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL, FOCAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- SEVERE, DIFFUSE ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATE, MULTI- FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT LUNG (LU) : LUNG (LU) : -UNCOLLAPSED; ALL LOBES >UNREMARKABLE HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL 1662 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 64 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90733 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 10:37 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL PARATHYROID (PT) : -HYPERPLASIA,-MODERATE, DIFFUSE STOMACH, GL (ST) : -EROSION,-MODERATE, FOCAL -MINERALIZATION,-MODERATE, MULTI-FOCAL JEJUNUM (JE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL CAUSE OF DEATH (CD) : -CHRONIC PROGRESSIVE NEPHROPATHY,- PRESENT TAIL (TI) : TAIL (TI) : -RAISED AREA(S); ENTIRE LENGTH OF -CYST, EPIDERMAL INCLUSION,-PRESENT TAIL: MULTIPLE TAN AND SLIGHTLY RAISED -EROSION,-SLIGHT, FOCAL AREAS, UP TO 15 X 10 X 2 MM; PORTION -INFLAMMATION, ACUTE,-MODERATE, MULTI- COLLECTED FOCAL -------------------------------------------- 1663 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 65 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90733 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 10:37 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1664 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 66 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90734 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 561.0 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 13:05 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK EYE (EY) : EYE (EY) : 61; 20X20 MM; VERIFIED AT NECROPSY: -OPAQUE CORNEA; BILATERAL -INFLAMMATION, ACUTE,-MODERATE, MULTI- OBSERVED FOCAL >APPEARANCE; THIN; VERIFIED AT -MINERALIZATION, CORNEAL,-MODERATE, NECROPSY: OBSERVED FOCAL >EYES; RED DISCHARGE; EYE-RIGHT; -ULCERATION, CORNEAL,-MODERATE, FOCAL VERIFIED AT NECROPSY: OBSERVED BRAIN (BR) : BRAIN (BR) : >EXCRETION; NO FECES; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, NECROPSY: NOT APPLICABLE EOSINOPHILIC,-MINIMAL, FOCAL >SKIN & PELAGE; COLD TO TOUCH; ENTIRE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- BODY; VERIFIED AT NECROPSY: NOT FOCAL APPLICABLE KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- -DIFFUSELY LIGHT; BILATERAL: TAN SEVERE, DIFFUSE TESTIS (TE) : -INFLAMMATION, VASCULAR,-MINIMAL, MULTI- FOCAL LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES, -INFILTRATE, MACROPHAGE, ALVEOLAR,- DIFFUSE: MULTIPLE WHITE AREAS, UP TO 2 MINIMAL, MULTI-FOCAL X 3 MM -MINERALIZATION,-MODERATE, MULTI-FOCAL 1665 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 67 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90734 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 561.0 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 13:05 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL -MINERALIZATION,-MODERATE, MULTI-FOCAL PARATHYROID (PT) : -HYPERPLASIA,-MODERATE, DIFFUSE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 5 X 6 X 8 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL FIRM, LOBULATED, RED, DARK RED, TAN, -B-ADENOMA,-NEOPLASM PRESENT AND BROWN STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: -EDEMA,-MINIMAL, MULTI-FOCAL MULTIPLE DARK RED AREAS, PINPOINT AND -MINERALIZATION,-MODERATELY SEVERE, UP TO 1 X 2 MM MULTI-FOCAL -ULCERATION,-MODERATE, FOCAL CECUM (CE) : CECUM (CE) : -RED FOCUS(I)/AREA(S); MUCOSA: -EDEMA,-SLIGHT, MULTI-FOCAL MULTIPLE DARK RED LINEAR AREAS, UP TO -EROSION,-MODERATE, MULTI-FOCAL 4 X 7 MM -HEMORRHAGE,-SLIGHT, MULTI-FOCAL URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,- SLIGHT, DIFFUSE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE, MULTI-FOCAL CAUSE OF DEATH (CD) : -CHRONIC PROGRESSIVE NEPHROPATHY,- PRESENT 1666 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 68 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90734 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 561.0 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 13:05 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; BILATERAL: LEFT IS 4 X 5 X 8 -DILATATION, SINUSOIDAL,-MODERATE, MM, RIGHT IS 2 X 3 X 5 MM (CAP 23) MULTI-FOCAL -MOTTLED; BILATERAL: EACH IS RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, AND TAN (CAP 23) MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); LEFT AXILLARY REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 13 X 17 X 24 MM, FIRM, LOBULATED, RED, AND TAN (CAP 21); SAME ON CUT SURFACE; REGIONAL LN (AXILLARY) NORMAL (CAP 22) AORTA (AO) : AORTA (AO) : -LARGE; DIFFUSE: UP TO 4 MM IN -MINERALIZATION,-SEVERE, MULTI-FOCAL DIAMETER -THICKENED WALL; DIFFUSE GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST BROWN MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 1667 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 69 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90734 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 561.0 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 13:05 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 1668 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 70 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90735 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 682.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:42 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: NOT OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: >UNREMARKABLE MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1669 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 71 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90735 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 682.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:42 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1670 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 72 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90736 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/13/99 STUDY DAY OF DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 511.0 GRAMS DATE AND TIME OF NECROPSY: 06/13/99 7:58 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >BEHAVIOR; HYPOACTIVE; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; HUNCHED; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE KIDNEY (KD) : KIDNEY (KD) : >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED -LARGE PELVIS(ES); BILATERAL -CAST, PROTEINACEOUS,-SLIGHT, MULTI- AT NECROPSY: NOT APPLICABLE FOCAL -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI- FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 19 X 7 X 7 MM, -B-ADENOMA,-NEOPLASM PRESENT RED, DARK RED, TAN AND SOFT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,- URETHRA: TAN AND FIRM MODERATELY SEVERE, MULTI-FOCAL 1671 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 73 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90736 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/13/99 STUDY DAY OF DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 511.0 GRAMS DATE AND TIME OF NECROPSY: 06/13/99 7:58 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); ALL REGIONS, DIFFUSE: -GALACTOCELE,-MODERATE, FOCAL MULTIPLE WHITE AND TAN, CREAMY, SEMIFLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 1672 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 74 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90736 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/13/99 STUDY DAY OF DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 511.0 GRAMS DATE AND TIME OF NECROPSY: 06/13/99 7:58 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1673 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 75 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90737 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 453.3 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 9:48 PROSECTOR: GREGORY CODDINGTON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0271 .0060 % .0126 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0299 .0066 % .0139 WEIGHT TAKEN BRAIN (BR) 2.1502 .4743 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.3893 .3065 % .6461 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.4361 .3168 % .6679 WEIGHT TAKEN LIVER (LI) 12.9059 2.8471 % 6.0022 WEIGHT TAKEN LUNG (LU) 1.4966 .3302 % .6960 WEIGHT TAKEN SPLEEN (SP) .8485 .1872 % .3946 WEIGHT TAKEN LEFT TESTIS (TE0) 1.4381 .3173 % .6688 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.4800 .3265 % .6883 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0090 .0020 % .0042 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0084 .0019 % .0039 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: COLLECTED >TISSUE MISSING GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 1674 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 76 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90737 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 453.3 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 9:48 PROSECTOR: GREGORY CODDINGTON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), DUODENUM (DU) ------------------------------------------------------------------------------------------------------------------------------------ 1675 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 77 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90738 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 689 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/15/99 14:58 PROSECTOR: WENDY CHERRY RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -CYST,-MODERATE, MULTI-FOCAL -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -OSSEOUS METAPLASIA,-MODERATE, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, DARK RED, TAN -B-ADENOMA,-NEOPLASM PRESENT AND SOFT 1676 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 78 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90738 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 689 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/15/99 14:58 PROSECTOR: WENDY CHERRY RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ JEJUNUM (JE) : >AUTOLYTIC PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, URETHRA: GREEN AND FIRM MULTI-FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); ALL REGIONS, DIFFUSE: -GALACTOCELE,-MODERATE, MULTI-FOCAL MULTIPLE TAN AND CREAMY SEMIFLUID -HEMORRHAGE,-SLIGHT, FOCAL FILLED CYSTS; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- 1677 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 79 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90738 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 689 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/15/99 14:58 PROSECTOR: WENDY CHERRY RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1678 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 80 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90739 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 714 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 598.2 GRAMS DATE AND TIME OF NECROPSY: 01/09/00 7:59 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; HUNCHED; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -RED FOCUS(I)/AREA(S); ALL LOBES: -VACUOLATION, HEPATOCELLULAR,-SEVERE, AT NECROPSY: NOT APPLICABLE MULTIPLE DARK RED AREAS, UP TO 2 MM IN DIFFUSE >EXCRETION; NO FECES; VERIFIED AT DIAMETER; SOME ARE SUNKEN WITH A NECROPSY: NOT APPLICABLE STELLATE PATTERN; COLLECTED ON ROUTINE >BEHAVIOR; HYPOACTIVE; VERIFIED AT SECTIONS NECROPSY: NOT APPLICABLE KIDNEY (KD) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -MINERALIZATION, PELVIC,-MINIMAL, MULTI- NECROPSY: OBSERVED FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, FOCAL TESTIS (TE) : TESTIS (TE) : -SMALL; LEFT: 16 X 10 X 3 MM (RIGHT -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE IS 20 X 15 X 5 MM); BOTH ARE SOFT -MINERALIZATION,-SLIGHT, FOCAL >NOTE:>LEFT TESTIS HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT, DIFFUSE 1679 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 81 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90739 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 714 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 598.2 GRAMS DATE AND TIME OF NECROPSY: 01/09/00 7:59 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 10 X 8 MM, RED, DARK -HEMORRHAGE,-MODERATE RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL PROSTATE (PR) : -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 5 X 2 -ONE EXAMINED,-PRESENT MM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: MOIST RED MATERIAL -------------------------------------------- 1680 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 82 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90739 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 714 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 598.2 GRAMS DATE AND TIME OF NECROPSY: 01/09/00 7:59 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1681 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 83 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90740 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/26/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 522.6 GRAMS DATE AND TIME OF NECROPSY: 04/26/99 14:59 PROSECTOR: WENDY CHERRY RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : LEFT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; COLD TO TOUCH; ENTIRE -MOTTLED; ALL LOBES: DARK RED AND TAN -VACUOLATION, HEPATOCELLULAR,-MINIMAL, BODY; VERIFIED AT NECROPSY: NOT MULTI-FOCAL APPLICABLE KIDNEY (KD) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -CAST, PROTEINACEOUS,-SLIGHT, MULTI- NECROPSY: NOT APPLICABLE FOCAL >EXCRETION; FEW FECES; VERIFIED AT -MINERALIZATION, PELVIC,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 10 X 10 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL TAN, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE BROWN AREAS, UP TO 1 X 1 MM ILEUM (IL) : -EDEMA,-MINIMAL, DIFFUSE PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL 1682 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 84 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90740 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/26/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 522.6 GRAMS DATE AND TIME OF NECROPSY: 04/26/99 14:59 PROSECTOR: WENDY CHERRY RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MEDIASTINAL (ML) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE PRESENT ON THYMUS SECTION TAIL (TI) : TAIL (TI) : -CRUSTED AREA(S); ALONG ENTIRE LENGTH: -EROSION,-MODERATELY SEVERE, FOCAL MULTIPLE DARK RED AND DRY AREAS, UP TO -FIBROSIS,-MODERATE, FOCAL 5 X 4 MM; COLLECTED -INFLAMMATION, ACUTE,-MODERATELY SEVERE, FOCAL FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: -EROSION,-MODERATE, MULTI-FOCAL SINGLE DARK RED AREA, 6 X 3 MM; -INFLAMMATION, CHRONIC ACTIVE,- COLLECTED MODERATELY SEVERE, MULTI-FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -------------------------------------------- 1683 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 85 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90740 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/26/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 522.6 GRAMS DATE AND TIME OF NECROPSY: 04/26/99 14:59 PROSECTOR: WENDY CHERRY RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1684 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 86 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90741 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 696 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 497.3 GRAMS DATE AND TIME OF NECROPSY: 12/22/99 13:51 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); VENTRAL-THORACIC- BRAIN (BR) : BRAIN (BR) : RIGHT; WEEK 69; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : NECROPSY: NOT OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >SKIN & PELAGE; YELLOW HAIR COAT; FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR,-MODERATE, >EXCRETION; FEW FECES; VERIFIED AT MULTI-FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; -MINERALIZATION, PELVIC,-MODERATE, VERIFIED AT NECROPSY: OBSERVED MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE SLIGHT, MULTI-FOCAL -PYELONEPHRITIS,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL LUNG (LU) : LUNG (LU) : -ADHESION(S); ALL LOBES TO PLEURA: -FIBROSIS, PLEURAL/SUBPLEURAL,-SLIGHT, MULTIPLE GRAY FIBROUS ADHESIONS, MULTI-FOCAL COLLECTED INTACT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, FOCAL 1685 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 87 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90741 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 696 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 497.3 GRAMS DATE AND TIME OF NECROPSY: 12/22/99 13:51 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 10 X 8 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -EDEMA,-MODERATE, FOCAL TAN ERODED AREAS, UP TO 8 X 4 MM -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL SKIN (SK) : SKIN (SK) : -RAISED AREA(S); RIGHT VENTRAL -CYST, EPIDERMAL INCLUSION,-PRESENT THORACIC AREA: SINGLE TAN AREA, 7 X 7 X 5 MM (CAP 22) PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HIND FOOT: -EDEMA,-MODERATE, FOCAL SINGLE TAN AND DRY AREA, 6 X 6 X 4 MM; -INFLAMMATION, ACUTE,-SLIGHT, MULTI- COLLECTED; RIGHT NOT OBSERVED AT FOCAL NECROPSY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 1686 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 88 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90741 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 696 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 497.3 GRAMS DATE AND TIME OF NECROPSY: 12/22/99 13:51 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1687 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 89 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90742 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 778.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:21 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -CYST,-PRESENT -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN; -HEMORRHAGE,-MINIMAL, MULTI-FOCAL COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1688 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 90 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90742 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 778.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:21 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1689 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 91 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90743 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1053.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:06 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -CYST,-MODERATELY SEVERE, FOCAL MULTIPLE DARK RED AREAS, UP TO 3 MM IN -TELANGIECTASIS,-MINIMAL, FOCAL DIAMETER; COLLECTED ON ROUTINE -VACUOLATION, HEPATOCELLULAR,-SLIGHT, SECTIONS MULTI-FOCAL -CYST(S); ALONG MARGIN OF LATERAL LOBE: SINGLE CLEAR FLUID FILLED CYST, 6 MM IN DIAMETER; COLLECTED KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL PANCREAS (PA) : -HYPERPLASIA, ACINAR,-MODERATE, MULTI- FOCAL ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-SLIGHT, FOCAL -VACUOLATION,-MINIMAL, FOCAL THYROID (TY) : -PIGMENT,-MINIMAL, FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, FOCAL -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT 1690 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 92 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90743 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1053.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:06 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 1691 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 93 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90743 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1053.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:06 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1692 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 94 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90744 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 549.6 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 11:22 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NO FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: OBSERVED -HEMORRHAGE,-MODERATE, FOCAL >EYES; SQUINTED; PERI-ORBITAL; VERIFIED KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -INFARCT,-SLIGHT, FOCAL >APPEARANCE; HUNCHED; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE MODERATELY SEVERE, DIFFUSE >SKIN & PELAGE; RED HAIR COAT; LIMBS- ADRENAL, CORTEX (AC) : FRONT; VERIFIED AT NECROPSY: OBSERVED -ANGIECTASIS,-MINIMAL, FOCAL >DISCHARGE; NASAL; RED IN COLOR; -HEMORRHAGE,-MINIMAL, FOCAL VERIFIED AT NECROPSY: OBSERVED HEART (HT) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -DEGENERATION/NECROSIS,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 7 X 11 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL FIRM, RED, DARK RED AND TAN -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE >UNREMARKABLE TAN AND RED ERODED AREAS, UP TO 2 X 3 MM -THICKENED WALL; DIFFUSE PROSTATE (PR) : -FIBROSIS,-MODERATE, FOCAL 1693 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 95 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90744 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 549.6 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 11:22 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -DISCHARGE-NASAL; DRY RED MATERIAL -STAINS - SKIN/HAIRCOAT; HEAD AND FRONT LEGS: DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 1694 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 96 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90744 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 549.6 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 11:22 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1695 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 97 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90745 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 501.7 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:15 PROSECTOR: DAVID SCHUETTE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; SORE/SCAB; PAW-HIND- -DILATATION, VENTRICULAR,-PRESENT RIGHT; VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : >HOUSING,FOOD,WATER & MAINT.; TEETH -NEPHROPATHY, CHRONIC PROGRESSIVE,- CUT; VERIFIED AT NECROPSY: NOT MINIMAL, MULTI-FOCAL APPLICABLE ADRENAL, CORTEX (AC) : >APPEARANCE; THIN; VERIFIED AT -DEGENERATION, CYSTIC, CORTICAL,- NECROPSY: NOT OBSERVED MODERATE, FOCAL >SKIN & PELAGE; RED HAIR COAT; VENTRAL- -HEMORRHAGE,-MINIMAL, FOCAL ABDOMINAL; VERIFIED AT NECROPSY: NOT LN, MESENTERIC (MS) : OBSERVED -INFILTRATE, MACROPHAGE, PIGMENTED,- >EXCRETION; FEW FECES; VERIFIED AT MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE LUNG (LU) : >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED -INFILTRATE, MACROPHAGE, ALVEOLAR,- AT NECROPSY: NOT APPLICABLE MINIMAL, MULTI-FOCAL >APPEARANCE; HUNCHED; VERIFIED AT THYROID (TY) : NECROPSY: NOT APPLICABLE -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 9 X 6 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- RED, TAN AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL 1696 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 98 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90745 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 501.7 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:15 PROSECTOR: DAVID SCHUETTE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); RIGHT HIND -EDEMA,-MODERATE, FOCAL FOOT: SINGLE, MOIST, BROWN ABRADED -EROSION,-MODERATELY SEVERE, FOCAL AREA, 7 MM IN DIAMETER (CAP 21) -INFLAMMATION, ACUTE,-MODERATE, FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: UPPER INCISORS -------------------------------------------- 1697 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 99 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90745 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 501.7 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:15 PROSECTOR: DAVID SCHUETTE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1698 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 100 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90746 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 551.3 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:19 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0373 .0068 % .0173 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0361 .0065 % .0168 WEIGHT TAKEN BRAIN (BR) 2.1500 .3900 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.0638 .3744 % .9599 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.1022 .3813 % .9778 WEIGHT TAKEN LIVER (LI) 15.8931 2.8828 % 7.3921 WEIGHT TAKEN LUNG (LU) 1.7030 .3089 % .7921 WEIGHT TAKEN SPLEEN (SP) .8143 .1477 % .3787 WEIGHT TAKEN LEFT TESTIS (TE0) 1.6455 .2985 % .7653 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.7015 .3086 % .7914 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0109 .0020 % .0051 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0108 .0020 % .0050 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY,- MINIMAL, FOCAL -HEMORRHAGE,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL STOMACH, GL (ST) : -EDEMA,-MODERATE, MULTI-FOCAL STOMACH, NONGL (SU) : -EDEMA,-MODERATE, MULTI-FOCAL -EROSION,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 1699 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 101 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90746 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 551.3 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:19 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), PARATHYROID (PT) ------------------------------------------------------------------------------------------------------------------------------------ 1700 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 102 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90747 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 457.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:48 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 97; 80 X 60MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR NECROPSY: OBSERVED CELL,-MODERATE, FOCAL >EYES; RED DISCHARGE; EYE-RIGHT; -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO VERIFIED AT NECROPSY: OBSERVED NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT >APPEARANCE; HUNCHED; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL SPLEEN (SP) : SPLEEN (SP) : -LARGE; 50 X 15 X 7 MM; MARGINS APPEAR -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO ROUNDED NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -HYPERTROPHY, CORTICAL CELL,-MODERATE, FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN, MESENTERIC (MS) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT TESTIS (TE) : >UNREMARKABLE 1701 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 103 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90747 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 457.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:48 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TESTIS (TE) : -SMALL; BILATERAL: EACH IS 18 X 8 X 7 MM; BOTH APPEAR SOFT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 5 X 5 MM, DARK RED AND -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SOFT -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PROSTATE (PR) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT SEMINAL VESICLES (SV) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT EPIDIDYMIDES (EP) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS UP TO 13 X -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO 10 X 4 MM; COLLECTED NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 1702 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 104 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90747 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 457.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:48 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); RIGHT INGUINAL REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 85 X 50 X 35 MM, RED, -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO TAN, FIRM AND LOBULATED; SAME ON CUT NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED LN, POPLITEAL (PP) : LN, POPLITEAL (PP) : -LARGE; BILATERAL: EACH IS UP TO 10 X -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO 8 X 8 MM (CAP 22) NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DRY RED MATERIAL -ANIMAL THIN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1703 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90747 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 457.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:48 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 1704 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 106 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90748 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 504.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:58 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0312 .0062 % .0139 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0271 .0054 % .0121 WEIGHT TAKEN BRAIN (BR) 2.2436 .4447 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.8414 .3650 % .8207 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.9977 .3960 % .8904 WEIGHT TAKEN LIVER (LI) 15.6073 3.0936 % 6.9564 WEIGHT TAKEN LUNG (LU) 1.9152 .3796 % .8536 WEIGHT TAKEN SPLEEN (SP) .8258 .1637 % .3681 WEIGHT TAKEN LEFT TESTIS (TE0) 1.4841 .2942 % .6615 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.5291 .3031 % .6815 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0137 .0027 % .0061 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0119 .0024 % .0053 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 1705 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 107 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90748 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 504.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:58 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 1706 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 108 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90749 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 427.3 GRAMS DATE AND TIME OF NECROPSY: 11/03/99 8:09 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; YELLOW HAIR COAT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >EXCRETION; NO FECES; VERIFIED AT -MOTTLED; ALL LOBES: DARK RED AND TAN -DEGENERATION, CYSTIC,-MODERATE, MULTI- NECROPSY: NOT APPLICABLE FOCAL >SKIN & PELAGE; RED HAIR COAT; HEAD- -FOCUS, ALTERED HEPATOCELLULAR, ENTIRE; VERIFIED AT NECROPSY: OBSERVED EOSINOPHILIC,-SLIGHT, MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -VACUOLATION, HEPATOCELLULAR,-MODERATE, NECROPSY: NOT APPLICABLE MULTI-FOCAL >EYES; RED DISCHARGE; EYES; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: OBSERVED -CYST(S); RIGHT, CORTICAL SURFACE: -CYST,-MODERATE, FOCAL >EYES; PALE; EYES; VERIFIED AT SINGLE CLEAR FLUID FILLED CYST, 3 MM -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE IN DIAMETER SLIGHT, MULTI-FOCAL >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : AT NECROPSY: NOT APPLICABLE -MOTTLED; BILATERAL: DARK RED AND TAN -VACUOLATION,-SLIGHT, FOCAL >RESPIRATION; LABORED; VERIFIED AT LUNG (LU) : NECROPSY: NOT APPLICABLE -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL 1707 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 109 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90749 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 427.3 GRAMS DATE AND TIME OF NECROPSY: 11/03/99 8:09 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 5 X 4 MM, RED, TAN AND -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SOFT -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 17 X 9 X 3 >UNREMARKABLE MM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); ALL REGIONS, DIFFUSE: -GALACTOCELE,-MODERATE, MULTI-FOCAL MULTIPLE WHITE, CREAMY SEMIFLUID FILLED CYSTS, UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: DRY, YELLOW MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: DRY, RED MATERIAL -ANIMAL THIN -------------------------------------------- 1708 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 110 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90749 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 427.3 GRAMS DATE AND TIME OF NECROPSY: 11/03/99 8:09 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1709 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 111 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90750 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 819.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:42 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); LEFT LATERAL -DEGENERATION, CYSTIC,-MINIMAL, FOCAL >WART LIKE LESION(S); DORSAL; WEEK 105; LOBE: MULTIPLE DARK RED AREAS, UP TO 3 -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- VERIFIED AT NECROPSY: OBSERVED MM IN DIAMETER; COLLECTED ON ROUTINE FOCAL SECTION -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-SLIGHT, MULTI- FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -LARGE; UP TO 15 MM IN DIAMETER -NECROSIS,-MODERATE, MULTI-FOCAL -DIFFUSELY RED; CAUDAL END -M-HEMANGIOSARCOMA,-NEOPLASM PRESENT TESTIS (TE) : -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL 1710 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 112 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90750 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 819.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:42 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : SKIN (SK) : -RAISED AREA(S); DORSAL THORACIC -CYST, EPIDERMAL INCLUSION,-PRESENT REGION: SINGLE DARK RED AND DRY AREA, 7 X 4 X 4 MM (CAP 23) PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: EACH IS UP TO 12 X -DILATATION, SINUSOIDAL,-MODERATE, 8 X 6 MM (CAP 22) MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL, >TISSUE MISSING HINDFEET: SINGLE DARK RED AND TAN ABRADED AREA ON EACH, LEFT IS 5 MM IN DIAMETER, RIGHT IS 4 MM IN DIAMETER; BOTH COLLECTED >NOTE:>TRIMMING COMMENT: MISSING FROM WET TISSUE AT TRIMMING GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1711 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 113 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90750 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 819.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:42 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1712 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 114 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90751 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 708.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:05 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0360 .0051 % .0162 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0279 .0039 % .0125 WEIGHT TAKEN BRAIN (BR) 2.2280 .3147 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.2013 .3109 % .9880 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.2651 .3199 % 1.0167 WEIGHT TAKEN LIVER (LI) 21.2216 2.9974 % 9.5250 WEIGHT TAKEN LUNG (LU) 2.4259 .3426 % 1.0888 WEIGHT TAKEN SPLEEN (SP) 1.1535 .1629 % .5177 WEIGHT TAKEN LEFT TESTIS (TE0) 1.4942 .2110 % .6706 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.5043 .2125 % .6752 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0167 .0024 % .0075 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0196 .0028 % .0088 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1713 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 115 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90751 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 708.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:05 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1714 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 116 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90751 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 708.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:05 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1715 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 117 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90752 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 763.5 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:53 PROSECTOR: JODI THIEREN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0389 .0051 % .0150 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0389 .0051 % .0150 WEIGHT TAKEN BRAIN (BR) 2.5855 .3386 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.6228 .3435 % 1.0144 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.5076 .3284 % .9699 WEIGHT TAKEN LIVER (LI) 21.9195 2.8709 % 8.4779 WEIGHT TAKEN LUNG (LU) 2.2547 .2953 % .8721 WEIGHT TAKEN SPLEEN (SP) .9130 .1196 % .3531 WEIGHT TAKEN LEFT TESTIS (TE0) 1.5675 .2053 % .6063 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.5833 .2074 % .6124 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0157 .0021 % .0061 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0185 .0024 % .0072 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI- FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -DARK FOCUS(I)/AREA(S); BILATERAL: TWO -HYPERTROPHY, CORTICAL CELL,-MINIMAL, DARK BROWN FOCI ON EACH, ALL ARE 1 MM FOCAL IN DIAMETER -VACUOLATION,-MINIMAL, FOCAL 1716 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 118 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90752 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 763.5 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:53 PROSECTOR: JODI THIEREN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1717 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 119 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90752 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 763.5 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:53 PROSECTOR: JODI THIEREN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1718 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 120 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90753 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 345.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:20 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 10.4324 3.0178 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1719 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 121 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90753 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 345.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:20 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 1720 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 122 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90754 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 789.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:33 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0315 .0040 % .0124 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0301 .0038 % .0119 WEIGHT TAKEN BRAIN (BR) 2.5326 .3207 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.4118 .3054 % .9523 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.4771 .3137 % .9781 WEIGHT TAKEN LIVER (LI) 20.9861 2.6578 % 8.2864 WEIGHT TAKEN LUNG (LU) 2.1265 .2693 % .8397 WEIGHT TAKEN SPLEEN (SP) 1.0063 .1274 % .3973 WEIGHT TAKEN LEFT TESTIS (TE0) 1.7176 .2175 % .6782 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.7539 .2221 % .6925 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0179 .0023 % .0071 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0257 .0033 % .0101 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; -HEMORRHAGE,-MODERATE, MULTI-FOCAL COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN 1721 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 123 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90754 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 789.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:33 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1722 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 124 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90755 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 695 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/21/99 13:33 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : LEFT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- -ROUGH SURFACE, DIFFUSE; BILATERAL SEVERE, DIFFUSE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, FOCAL -DEGENERATION, CYSTIC, CORTICAL,- SLIGHT, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL -PIGMENT,-MODERATE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT 1723 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 125 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90755 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 695 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/21/99 13:33 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 8 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GRAY, AND SOFT PROSTATE (PR) : -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT HIND -EDEMA,-MODERATE, FOCAL FOOT: SINGLE TAN AND MOIST ABRADED -EROSION,-MODERATELY SEVERE, FOCAL AREA, 7 X 7 X 6 MM; COLLECTED -EXOSTOSES,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, FOCAL GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- 1724 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 126 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90755 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 695 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/21/99 13:33 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1725 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 127 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90756 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 386.2 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 11:00 PROSECTOR: WENDY CHERRY RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0348 .0090 % .0161 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0326 .0084 % .0150 WEIGHT TAKEN BRAIN (BR) 2.1666 .5610 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.3350 .3457 % .6162 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.2983 .3362 % .5992 WEIGHT TAKEN LIVER (LI) 8.8391 2.2887 % 4.0797 WEIGHT TAKEN LUNG (LU) 1.3439 .3480 % .6203 WEIGHT TAKEN SPLEEN (SP) .4973 .1288 % .2295 WEIGHT TAKEN LEFT TESTIS (TE0) 1.3216 .3422 % .6100 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.3094 .3390 % .6044 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0002 .0001 % .0001 EXCLUDE RIGHT THYROID/PR (TT1) .0052 .0013 % .0024 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LUNG (LU) : LUNG (LU) : -MOTTLED; TRIMMING COMMENT: LEFT LOBE >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CRANIAL HALF: RED AND TAN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN 1726 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 128 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90756 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 386.2 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 11:00 PROSECTOR: WENDY CHERRY RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), DUODENUM (DU) ------------------------------------------------------------------------------------------------------------------------------------ 1727 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 129 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90757 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/28/99 STUDY DAY OF DEATH: 488 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 659.6 GRAMS DATE AND TIME OF NECROPSY: 05/28/99 7:36 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; SWOLLEN; LIMB-HIND-LEFT; -DILATATION, VENTRICULAR,-PRESENT VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : >RESPIRATION; AUDIBLE; VERIFIED AT -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED -VACUOLATION, HEPATOCELLULAR,-MODERATE, AT NECROPSY: NOT APPLICABLE MULTI-FOCAL >SKIN & PELAGE; RED HAIR COAT; VENTRAL- KIDNEY (KD) : ABDOMINAL; VERIFIED AT NECROPSY: -NEPHROPATHY, CHRONIC PROGRESSIVE,- OBSERVED MINIMAL, MULTI-FOCAL -PYELONEPHRITIS,-MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL TESTIS (TE) : TESTIS (TE) : -SMALL; LEFT: 13 X 9 X 7 MM (FOR -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE COMPARISON, RIGHT IS 21 X 15 X 9 MM); LEFT IS SOFT THYROID (TY) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 1728 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 130 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90757 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/28/99 STUDY DAY OF DEATH: 488 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 659.6 GRAMS DATE AND TIME OF NECROPSY: 05/28/99 7:36 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 9 X 8 MM, RED ,DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GREY, TAN, LOBULATED AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE RED AND DARK RED AREAS, UP TO 3 X 2 MM -THICKENED WALL; MUCOSA STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; MUCOSA -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATELY SEVERE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL EPIDIDYMIDES (EP) : EPIDIDYMIDES (EP) : -SMALL; LEFT: 31 X 5 X 4 MM (FOR >UNREMARKABLE COMPARISON, RIGHT IS 51 X 8 X 7 MM) CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; LEFT TARSAL AND METATARSAL: -DISLOCATION,-PRESENT TARSAL IS 14 X 12 X 11 MM, METATARSAL -EDEMA,-MODERATE, MULTI-FOCAL IS 15 X 13 X 8 MM; TISSUE SURROUNDING -HEMORRHAGE,-MINIMAL, MULTI-FOCAL BOTH APPEARS SWOLLEN; BOTH COLLECTED -INFLAMMATION, CHRONIC-ACTIVE, SYNOVIAL,-MODERATE, MULTI-FOCAL 1729 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 131 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90757 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/28/99 STUDY DAY OF DEATH: 488 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 659.6 GRAMS DATE AND TIME OF NECROPSY: 05/28/99 7:36 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS, EXTENDING TO ABDOMINAL REGION: DRY, RED MATERIAL -BLOOD FILM TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1730 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 132 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90758 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 707.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:37 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: NOT OBSERVED -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC -DEGENERATION, CYSTIC,-MINIMAL, FOCAL SURFACE OF LEFT LATERAL LOBE: SINGLE -FOCUS, ALTERED HEPATOCELLULAR, RED AREA, 3 MM IN DIAMETER; COLLECTED EOSINOPHILIC,-SLIGHT, FOCAL ON ROUTINE SECTION KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL PANCREAS (PA) : PANCREAS (PA) : -LARGE VESSELS; DIFFUSE: UP TO 3 MM IN -HYPERTROPHY, VASCULAR,-MODERATE, MULTI- DIAMETER (CAP 22) FOCAL -INFLAMMATION, VASCULAR,-SLIGHT, MULTI- FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -VACUOLATION,-SLIGHT, MULTI-FOCAL RECTUM (RE) : -PARASITISM,-PRESENT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING >UNREMARKABLE URETHRA: GREEN AND FIRM 1731 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 133 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90758 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 707.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:37 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1732 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 134 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90758 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 707.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:37 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1733 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 135 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90759 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 597 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 332.4 GRAMS DATE AND TIME OF NECROPSY: 09/14/99 10:50 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; ROUGH HAIR COAT; -MOTTLED; ALL LOBES: BROWN AND DARK -HEMORRHAGE,-SLIGHT, MULTI-FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE RED -NECROSIS, HEPATOCELLULAR,-MODERATE, >SKIN & PELAGE; YELLOW HAIR COAT; MULTI-FOCAL VERIFIED AT NECROPSY: NOT OBSERVED KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 10 X 9 MM, RED, DARK -HEMORRHAGE,-SLIGHT, MULTI-FOCAL RED, TAN, GREY AND SOFT -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING >UNREMARKABLE URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; LEFT: 30 X 15 X 7 MM (RIGHT IS >UNREMARKABLE 20 X 10 X 5 MM) 1734 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 136 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90759 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 597 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 332.4 GRAMS DATE AND TIME OF NECROPSY: 09/14/99 10:50 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -GALACTOCELE,-MODERATE, MULTI-FOCAL AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 1735 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 137 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90759 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 597 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 332.4 GRAMS DATE AND TIME OF NECROPSY: 09/14/99 10:50 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1736 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 138 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90760 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/10/99 STUDY DAY OF DEATH: 593 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/10/99 10:15 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; EYE (EY) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, ACUTE,-SLIGHT, MULTI- >EYES; DISCOLORED/LIGHT RED; EYE-LEFT; FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : KIDNEY (KD) : >SKIN & PELAGE; YELLOW HAIR COAT; -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE -CALCULUS(I); RIGHT, WITHIN PELVIS: -MINERALIZATION, PELVIC,-SLIGHT, MULTI- MULTIPLE TAN AND FIRM CALCULI, UP TO FOCAL 10 X 6 X 5 MM -NECROSIS, PAPILLARY,-SEVERE, DIFFUSE -GRANULAR MATERIAL; LEFT, WITHIN -PYELONEPHRITIS,-SEVERE, MULTI-FOCAL PELVIS: MULTIPLE TAN PINPOINT GRANULES -LARGE; BILATERAL: EACH IS 35 X 24 X 16 MM -ADHESION(S); RIGHT, CORTICAL SURFACE TO RIGHT LATERAL AND CAUDATE LOBES OF LIVER: MULTIPLE GREY FIBROUS ADHESIONS; COLLECTED INTACT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- SLIGHT, MULTI-FOCAL -VACUOLATION,-SLIGHT, FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT 1737 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 139 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90760 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/10/99 STUDY DAY OF DEATH: 593 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/10/99 10:15 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MODERATE, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); LEFT POSTERIOR ASPECT: -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 3 MM IN DIAMETER, RED, -B-ADENOMA,-NEOPLASM PRESENT DARK RED AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EROSION,-MINIMAL, MULTI-FOCAL MULTIPLE DARK BROWN AREAS, PINPOINT AND UP TO 1 MM URINARY BLADDER (UB) : URINARY BLADDER (UB) : -THICKENED WALL; PROXIMAL END: TAN AND -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FIRM FOCAL -CALCULUS(I); WITHIN LUMEN: MULTIPLE TAN CALCULI, UP TO 7 MM IN DIAMETER PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 12 X 8 X 2 >UNREMARKABLE MM EPIDIDYMIDES (EP) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL 1738 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 140 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90760 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/10/99 STUDY DAY OF DEATH: 593 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/10/99 10:15 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PYELONEPHRITIS,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; LEFT: UP TO 20 X 15 X 4 MM -DILATATION, SINUSOIDAL,-MODERATE, (RIGHT IS NOT IDENTIFIED) (CAP 23) MULTI-FOCAL LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; LEFT: 10 X 10 X 6 MM (RIGHT IS >TISSUE MISSING NOT IDENTIFIED) (CAP 22) >NOTE:>RENAL LYMPH NODE DESCRIBED GROSSLY WAS ADIPOSE TISSUE FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); BILATERAL, HINDFEET: -EROSION,-SEVERE, MULTI-FOCAL SINGLE DARK RED CRUSTED AREA ON EACH, -EXOSTOSES,-MODERATELY SEVERE, MULTI- LEFT IS 12 X 10 MM, RIGHT IS 17 X 12 FOCAL MM; BOTH COLLECTED -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL ADIPOSE TISSUE (AT) : -INFLAMMATION, CHRONIC ACTIVE,- MODERATELY SEVERE, MULTI-FOCAL GENERAL COMMENT (GC) : -URINARY BLADDER-LARGE LUMEN; UP TO 30 MM IN DIAMETER; FILLED WITH A DARK BROWN FLUID -------------------------------------------- 1739 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 141 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90760 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/10/99 STUDY DAY OF DEATH: 593 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/10/99 10:15 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1740 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 142 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90761 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 662 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 13:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; THYMUS (TH) : VERIFIED AT NECROPSY: OBSERVED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-SLIGHT, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 4 X 3 MM, RED, GREY, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC RECTUM (RE) : -PARASITISM,-PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE 1741 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 143 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90761 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 662 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 13:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1742 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 144 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90762 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/27/99 STUDY DAY OF DEATH: 548 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/27/99 8:12 PROSECTOR: LYNN HORNER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; LUNG (LU) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; >UNREMARKABLE COLLECTED FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL, -FIBROSIS,-SEVERE, MULTI-FOCAL HINDFEET: SINGLE DRY DARK RED ABRADED -INFLAMMATION, ACUTE,-SLIGHT, MULTI- AREA ON EACH, RIGHT IS 8 X 6 MM, LEFT FOCAL IS 5 X 4 MM; BOTH COLLECTED -------------------------------------------- 1743 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 145 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90762 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/27/99 STUDY DAY OF DEATH: 548 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/27/99 8:12 PROSECTOR: LYNN HORNER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1744 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 146 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90763 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 628.5 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 11:45 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYE-LEFT; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >SKIN & PELAGE; RED HAIR COAT; HEAD- FOCAL MAXILLARY-LEFT; VERIFIED AT NECROPSY: KIDNEY (KD) : KIDNEY (KD) : NOT OBSERVED -LARGE PELVIS(ES); BILATERAL -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >APPEARANCE; LIMITED USE; LIMBS-HIND; FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE -DILATATION, PELVIC,-PRESENT >APPEARANCE; LIMITED USE; LIMBS-FRONT; -MINERALIZATION, PELVIC,-SLIGHT, MULTI- VERIFIED AT NECROPSY: NOT APPLICABLE FOCAL >BEHAVIOR; ATAXIC; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: NOT APPLICABLE -DEGENERATION, CYSTIC, CORTICAL,- SLIGHT, FOCAL -HEMORRHAGE,-MINIMAL, FOCAL THYMUS (TH) : -CYST,-PRESENT -HEMORRHAGE,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL MUSCLE, SKELETAL (SM) : -DEGENERATION/NECROSIS,-MODERATE, MULTI- FOCAL 1745 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 147 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90763 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 628.5 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 11:45 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 1746 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 148 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90763 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 628.5 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 11:45 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1747 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 149 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90764 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/04/99 STUDY DAY OF DEATH: 587 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/04/99 10:28 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK KIDNEY (KD) : 84; VERIFIED AT NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL PANCREAS (PA) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT THYMUS (TH) : -CYST,-PRESENT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); RIGHT AXILLARY REGION: >TISSUE MISSING SINGLE MASS, 10 X 8 X 4 MM, TAN AND >NOTE:>MAMMARY MASS DESCRIBED GROSSLY FIRM; SAME ON CUT SURFACE; COLLECTED; WAS A SUBCUTANEOUS REGIONAL LN (AXILLARY) NORMAL (CAP 21) KERATOACANTHOMA -------------------------------------------- 1748 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 150 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90764 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/04/99 STUDY DAY OF DEATH: 587 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/04/99 10:28 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 1749 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 151 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90765 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 888.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:59 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -DEGENERATION, CYSTIC,-MODERATELY MULTIPLE DARK RED AREAS, UP TO 2 MM IN SEVERE, MULTI-FOCAL DIAMETER; COLLECTED ON ROUTINE -FOCUS, ALTERED HEPATOCELLULAR, SECTIONS EOSINOPHILIC,-MODERATE, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -INFLAMMATION, ACUTE,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL -INFILTRATE, EOSINOPHILS,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, MULTI-FOCAL LN, MESENTERIC (MS) : -THROMBUS,-SLIGHT, FOCAL 1750 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 152 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90765 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 888.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:59 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -FIBROSIS, PLEURAL/SUBPLEURAL,- MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); RIGHT ANTERIOR -HEMORRHAGE,-SLIGHT, MULTI-FOCAL ASPECT: SINGLE DARK BROWN AREA, 2 MM -B-ADENOMA,-NEOPLASM PRESENT IN DIAMETER URINARY BLADDER (UB) : -B-PAPILLOMA, TRANSITIONAL CELL,- NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1751 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 153 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90765 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 888.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:59 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1752 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 154 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90766 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/10/99 STUDY DAY OF DEATH: 440 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 601.8 GRAMS DATE AND TIME OF NECROPSY: 04/10/99 14:02 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >BEHAVIOR; HYPOACTIVE; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >RESPIRATION; AUDIBLE; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >RESPIRATION; LABORED; VERIFIED AT -CAST, PROTEINACEOUS,-SLIGHT, MULTI- NECROPSY: NOT APPLICABLE FOCAL >RESPIRATION; IRREGULAR; VERIFIED AT LUNG (LU) : NECROPSY: NOT APPLICABLE -INFLAMMATION, ACUTE,-MODERATE, MULTI- >SKIN & PELAGE; COLD TO TOUCH; ENTIRE FOCAL BODY; VERIFIED AT NECROPSY: NOT THYROID (TY) : APPLICABLE -CYST, ULTIMOBRANCHIAL,-PRESENT >SKIN & PELAGE; ROUGH HAIR COAT; -B-ADENOMA, C-CELL,-NEOPLASM PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 9 X 9 MM, RED, DARK -HEMORRHAGE,-MINIMAL, MULTI-FOCAL RED, GRAY, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -DISCHARGE-ORAL; DRY, RED MATERIAL -BLOOD FILM TAKEN 1753 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 155 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90766 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/10/99 STUDY DAY OF DEATH: 440 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 601.8 GRAMS DATE AND TIME OF NECROPSY: 04/10/99 14:02 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1754 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 156 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90767 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 710.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:34 PROSECTOR: JODI THIEREN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0366 .0051 % .0160 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0340 .0048 % .0149 WEIGHT TAKEN BRAIN (BR) 2.2806 .3208 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.1832 .3071 % .9573 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.2020 .3098 % .9655 WEIGHT TAKEN LIVER (LI) 22.1916 3.1221 % 9.7306 WEIGHT TAKEN LUNG (LU) 1.7726 .2494 % .7773 WEIGHT TAKEN SPLEEN (SP) .9195 .1294 % .4032 WEIGHT TAKEN LEFT TESTIS (TE0) 1.7098 .2405 % .7497 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.6666 .2345 % .7308 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0146 .0021 % .0064 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0173 .0024 % .0076 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MINIMAL, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 1755 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 157 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90767 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 710.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:34 PROSECTOR: JODI THIEREN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY -------------------------------------------- 1756 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 158 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90767 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 710.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:34 PROSECTOR: JODI THIEREN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1757 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 159 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90768 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 457.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:25 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0276 .0060 % .0124 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0281 .0061 % .0127 WEIGHT TAKEN BRAIN (BR) 2.2186 .4846 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.7442 .3810 % .7862 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.7623 .3849 % .7943 WEIGHT TAKEN LIVER (LI) 12.7081 2.7759 % 5.7280 WEIGHT TAKEN LUNG (LU) 1.7101 .3735 % .7708 WEIGHT TAKEN SPLEEN (SP) .4316 .0943 % .1945 WEIGHT TAKEN LEFT TESTIS (TE0) 1.9313 .4219 % .8705 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.8487 .4038 % .8333 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0103 .0022 % .0046 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0109 .0024 % .0049 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-SLIGHT, FOCAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT -HYPERPLASIA, TRANSITIONAL CELL,- MINIMAL, MULTI-FOCAL -PYELONEPHRITIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 1758 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 160 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90768 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 457.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:25 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 21) GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 1759 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 161 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90769 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 460.2 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:41 PROSECTOR: DONIA MEYER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0272 .0059 % .0123 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0247 .0054 % .0112 WEIGHT TAKEN BRAIN (BR) 2.2138 .4811 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.4498 .3150 % .6549 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.4769 .3209 % .6671 WEIGHT TAKEN LIVER (LI) 12.3579 2.6853 % 5.5822 WEIGHT TAKEN LUNG (LU) 1.6728 .3635 % .7556 WEIGHT TAKEN SPLEEN (SP) .7676 .1668 % .3467 WEIGHT TAKEN LEFT TESTIS (TE0) 1.5958 .3468 % .7208 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.5866 .3448 % .7167 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0133 .0029 % .0060 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0154 .0033 % .0070 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CYST,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 1760 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 162 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90769 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 460.2 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:41 PROSECTOR: DONIA MEYER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 1761 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 163 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90770 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 379.4 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 15:10 PROSECTOR: JOHN S. HALFORD RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; YELLOW HAIR COAT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; NO FECES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -VACUOLATION, HEPATOCELLULAR,-MODERATE, NECROPSY: OBSERVED MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >RESPIRATION; LABORED; VERIFIED AT SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -SMALL; UP TO 3 MM IN DIAMETER >UNREMARKABLE THYMUS (TH) : THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT >UNREMARKABLE POSITIVELY IDENTIFIED; POSSIBLE REMNANTS COLLECTED THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SEVERE, FOCAL 1762 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 164 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90770 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 379.4 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 15:10 PROSECTOR: JOHN S. HALFORD RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 14 X 8 X 8 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, LOBULATED, AND SOFT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA, >UNREMARKABLE SURROUNDING PYLORIC VALVE: MULTIPLE TAN, AND SLIGHTLY ERODED AREAS, UP TO 2 X 1 MM STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -EDEMA,-SLIGHT, FOCAL TAN ERODED AREAS, UP TO 3 X 1 MM -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, FOCAL -ULCERATION,-MODERATE, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL ABDOMINAL REGION: DRY BROWN MATERIAL -ANIMAL THIN 1763 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 165 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90770 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 379.4 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 15:10 PROSECTOR: JOHN S. HALFORD RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1764 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 166 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90771 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/11/99 STUDY DAY OF DEATH: 655 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 439.3 GRAMS DATE AND TIME OF NECROPSY: 11/11/99 7:16 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : LEFT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: OBSERVED LIVER (LI) : >DISCHARGE; NASAL; RED IN COLOR; -DEGENERATION, CYSTIC,-MINIMAL, FOCAL VERIFIED AT NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >BEHAVIOR; HYPOACTIVE; VERIFIED AT EOSINOPHILIC,-MINIMAL, FOCAL NECROPSY: NOT APPLICABLE -HEMORRHAGE,-MINIMAL, FOCAL >EXCRETION; FEW FECES; VERIFIED AT -HYPERPLASIA, BILE DUCT,-SLIGHT, MULTI- NECROPSY: NOT APPLICABLE FOCAL >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED -VACUOLATION, HEPATOCELLULAR,-SLIGHT, AT NECROPSY: NOT APPLICABLE MULTI-FOCAL KIDNEY (KD) : KIDNEY (KD) : -DEPRESSED FOCUS(I)/AREA(S); RIGHT, -INFARCT,-MINIMAL, MULTI-FOCAL CORTICAL SURFACE: SINGLE DARK BROWN -NEPHROPATHY, CHRONIC PROGRESSIVE,- DEPRESSED AREA, 3 X 2 MM MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, FOCAL THYMUS (TH) : -CYST,-PRESENT THYROID (TY) : -INFLAMMATION, GRANULOMATOUS,-MODERATE, FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 1765 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 167 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90771 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/11/99 STUDY DAY OF DEATH: 655 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 439.3 GRAMS DATE AND TIME OF NECROPSY: 11/11/99 7:16 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 5 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL TAN, LOBULATED AND SOFT -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL STOMACH, NONGL (SU) : -EDEMA,-MODERATE, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; LEFT: 16 X 8 X 3 MM (RIGHT IS >UNREMARKABLE 21 X 11 X 5 MM) CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); BILATERAL PECTORAL REGIONS -GALACTOCELE,-MODERATE, MULTI-FOCAL AND RIGHT INGUINAL REGION: MULTIPLE WHITE, CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER (CAP 22) FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: -EROSION,-MODERATE, FOCAL SINGLE DRY, DARK RED CRUSTED AREA, 8 X -FIBROSIS,-MODERATE, FOCAL 5 MM; COLLECTED -INFLAMMATION, ACUTE,-MODERATE, FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL 1766 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 168 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90771 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/11/99 STUDY DAY OF DEATH: 655 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 439.3 GRAMS DATE AND TIME OF NECROPSY: 11/11/99 7:16 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-NASAL; DRY, RED MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1767 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 169 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90772 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/29/99 STUDY DAY OF DEATH: 428 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 651.7 GRAMS DATE AND TIME OF NECROPSY: 03/29/99 11:55 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; LIMITED USE; LIMBS-HIND; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -DIFFUSELY LIGHT; ALL LOBES: TAN >UNREMARKABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: OBSERVED -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT >SKIN & PELAGE; YELLOW HAIR COAT; -MOTTLED; BILATERAL: BROWN AND TAN -NEPHROPATHY, CHRONIC PROGRESSIVE,- VERIFIED AT NECROPSY: OBSERVED MINIMAL, MULTI-FOCAL LUNG (LU) : LUNG (LU) : -RED FOCUS(I)/AREA(S); DORSAL SURFACE >UNREMARKABLE OF LEFT LOBE: SINGLE DARK RED AREA, 6 X 22 MM MUSCLE, SKELETAL (SM) : -M-FIBROSARCOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH HORN IS 8 X 14 -EDEMA,-MODERATE, MULTI-FOCAL X 32 MM -INFLAMMATION, ACUTE,-MODERATE, MULTI- -MOTTLED; BILATERAL: TAN, YELLOW AND FOCAL RED CAUSE OF DEATH (CD) : -FIBROSARCOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL LUMBAR: EACH IS 12 X >TISSUE MISSING 12 X 27 MM (CAP 21) 1768 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 170 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90772 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/29/99 STUDY DAY OF DEATH: 428 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 651.7 GRAMS DATE AND TIME OF NECROPSY: 03/29/99 11:55 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL LUMBAR: RED AND >NOTE:>LUMBAR LYMPH NODE DESCRIBED TAN (CAP 21) GROSSLY WAS A FIBROSARCOMA OF SKELETAL MUSCLE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DRY YELLOW MATERIAL -URINARY BLADDER-LARGE LUMEN; 20 X 20 X 32 MM, FILLED WITH A CLEAR YELLOW FLUID -------------------------------------------- 1769 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 171 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90772 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/29/99 STUDY DAY OF DEATH: 428 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 651.7 GRAMS DATE AND TIME OF NECROPSY: 03/29/99 11:55 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1770 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 172 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90773 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/25/99 STUDY DAY OF DEATH: 455 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/25/99 8:23 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 6 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, NEAR URETHRA: -INFLAMMATION, ACUTE,-MODERATE, MULTI- GREEN AND FIRM FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -DISCHARGE-ORAL; RED -DISCHARGE-OCULAR; BILATERAL: RED -DISCHARGE-NASAL; BILATERAL: RED -ANIMAL OBESE -URINARY BLADDER-LARGE LUMEN; UP TO 19 MM IN DIAMETER -------------------------------------------- 1771 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 173 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90773 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/25/99 STUDY DAY OF DEATH: 455 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/25/99 8:23 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1772 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 174 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90774 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/09/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 402.3 GRAMS DATE AND TIME OF NECROPSY: 06/09/99 14:00 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; HEAD- BRAIN (BR) : BRAIN (BR) : MAXILLARY-LEFT; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NOT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; RED HAIR COAT; HEAD- LIVER (LI) : MAXILLARY-RIGHT; VERIFIED AT NECROPSY: -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL NOT OBSERVED KIDNEY (KD) : >SKIN & PELAGE; YELLOW HAIR COAT; -CAST, PROTEINACEOUS,-MINIMAL, MULTI- VERIFIED AT NECROPSY: NOT OBSERVED FOCAL >EXCRETION; FEW FECES; VERIFIED AT LUNG (LU) : NECROPSY: NOT APPLICABLE -EDEMA, ALVEOLAR,-SLIGHT, MULTI-FOCAL >APPEARANCE; THIN; VERIFIED AT -INFILTRATE, MACROPHAGE, ALVEOLAR,- NECROPSY: OBSERVED SLIGHT, FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT THYROID (TY) : NECROPSY: NOT APPLICABLE -MINERALIZATION,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 15 X 8 X 5 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, DARK RED, TAN, LOBULATED AND SOFT -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1773 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 175 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90774 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/09/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 402.3 GRAMS DATE AND TIME OF NECROPSY: 06/09/99 14:00 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, MALE (MM) : -PIGMENT,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY MATERIAL -DISCHARGE-NASAL; BILATERAL: DARK RED, DRY MATERIAL -ANIMAL THIN -------------------------------------------- 1774 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 176 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90774 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/09/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 402.3 GRAMS DATE AND TIME OF NECROPSY: 06/09/99 14:00 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1775 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 177 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90775 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 352.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:53 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 10.6997 3.0319 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1776 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 178 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90775 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 352.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:53 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 1777 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 179 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90776 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 696 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/22/99 7:59 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYMUS (TH) : >TISSUE MISSING HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC 1778 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 180 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90776 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 696 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/22/99 7:59 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ILEUM (IL) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); BILATERAL PECTORAL REGIONS: -GALACTOCELE,-MODERATE, MULTI-FOCAL MULTIPLE TAN AND CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER (CAP 22) GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- 1779 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 181 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90776 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 696 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/22/99 7:59 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1780 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 182 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90777 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/29/99 STUDY DAY OF DEATH: 612 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 482.9 GRAMS DATE AND TIME OF NECROPSY: 09/29/99 8:08 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- EYE (EY) : LEFT; VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, ACUTE,-MINIMAL, MULTI- >SKIN & PELAGE; RED HAIR COAT; VENTRAL- FOCAL THORACIC; VERIFIED AT NECROPSY: NOT BRAIN (BR) : BRAIN (BR) : OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; RED HAIR COAT; HEAD- -DILATATION, VENTRICULAR,-PRESENT ENTIRE; VERIFIED AT NECROPSY: NOT LIVER (LI) : OBSERVED -VACUOLATION, HEPATOCELLULAR,-SEVERE, >EYES; SQUINTED; PERI-ORBITAL; VERIFIED DIFFUSE AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -MINERALIZATION, PELVIC,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >EYES; PALE; EYES; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE MODERATELY SEVERE, DIFFUSE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, MULTI-FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT, DIFFUSE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 11 X 10 X 9 -HEMORRHAGE,-MODERATE, MULTI-FOCAL MM, RED, DARK RED, TAN, LOBULATED, AND -B-ADENOMA,-NEOPLASM PRESENT FIRM 1781 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 183 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90777 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/29/99 STUDY DAY OF DEATH: 612 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 482.9 GRAMS DATE AND TIME OF NECROPSY: 09/29/99 8:08 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EROSION,-MINIMAL, FOCAL MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EDEMA,-MINIMAL, MULTI-FOCAL MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 40 X 20 X 5 -SECRETION, INCREASED,-PRESENT MM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT -EROSION,-MODERATELY SEVERE, FOCAL HINDFOOT: SINGLE TAN, MOIST ABRADED -INFLAMMATION, ACUTE,-MODERATE, FOCAL AREA, 9 X 8 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 1782 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 184 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90777 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/29/99 STUDY DAY OF DEATH: 612 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 482.9 GRAMS DATE AND TIME OF NECROPSY: 09/29/99 8:08 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1783 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 185 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90778 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 707.3 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:41 PROSECTOR: MICHELE MARGGI RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; EYE (EY) : EYE (EY) : WEEK 82; 90 X 80MM; VERIFIED AT -OPAQUE CORNEA; BILATERAL: CLEAR -CATARACT,-PRESENT NECROPSY: OBSERVED KIDNEY (KD) : >EYES; OPAQUE; EYES; VERIFIED AT -CAST, PROTEINACEOUS,-MINIMAL, MULTI- NECROPSY: OBSERVED FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, FOCAL -HEMORRHAGE,-MODERATE, FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); RIGHT ASPECT: -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SINGLE DARK BROWN AREA, 3 X 1 MM -B-ADENOMA,-NEOPLASM PRESENT 1784 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 186 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90778 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 707.3 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:41 PROSECTOR: MICHELE MARGGI RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; RIGHT: 25 X 15 X 5 MM (LEFT IS -SECRETION, INCREASED,-PRESENT 20 X 10 X 4 MM); FIRM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -DILATATION, SINUSOIDAL,-MINIMAL, MULTI- FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS ON THYMUS SECTION MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); RIGHT INGUINAL REGION: -NECROSIS,-MODERATELY SEVERE, MULTI- SINGLE MASS, 120 X 90 X 40 MM, RED, FOCAL DARK RED, TAN, GREY, AND FIRM; SAME ON -B-FIBROADENOMA,-NEOPLASM PRESENT CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1785 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 187 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90778 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 707.3 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:41 PROSECTOR: MICHELE MARGGI RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1786 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 188 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90779 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 442.5 GRAMS DATE AND TIME OF NECROPSY: 11/03/99 14:02 PROSECTOR: JOHN S. HALFORD RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; OPAQUE; EYE-RIGHT; VERIFIED AT EYE (EY) : EYE (EY) : NECROPSY: OBSERVED -OPAQUE CORNEA; RIGHT -MINERALIZATION, LENS,-MINIMAL, FOCAL >SKIN & PELAGE; BROWN HAIR COAT; BRAIN (BR) : BRAIN (BR) : VENTRAL-ABDOMINAL; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; NO FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR,-MINIMAL, >APPEARANCE; THIN; VERIFIED AT MULTI-FOCAL NECROPSY: OBSERVED KIDNEY (KD) : KIDNEY (KD) : >SKIN & PELAGE; RED HAIR COAT; HEAD- -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- ENTIRE; VERIFIED AT NECROPSY: OBSERVED -DIFFUSELY LIGHT; BILATERAL: TAN MODERATELY SEVERE, DIFFUSE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED ADRENAL, CORTEX (AC) : AT NECROPSY: NOT APPLICABLE -VACUOLATION,-MODERATE, MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT ADRENAL, MEDULLA (MA) : NECROPSY: NOT APPLICABLE -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, RED, GRAY, -HEMORRHAGE,-MINIMAL, MULTI-FOCAL TAN, LOBULATED AND SOFT -B-ADENOMA,-NEOPLASM PRESENT 1787 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 189 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90779 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 442.5 GRAMS DATE AND TIME OF NECROPSY: 11/03/99 14:02 PROSECTOR: JOHN S. HALFORD RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SALIV GL, MANDIB (SG) : -MINERALIZATION,-MINIMAL, MULTI-FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTIPLE DARK BROWN AREAS, UP TO 3 X 2 MULTI-FOCAL MM -THICKENED WALL; MUCOSA STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; MUCOSA -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, TISSUE SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,- URETHRA: GREEN AND FIRM MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: CLEAR AND SOFT -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, FOCAL -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: BROWN MOIST MATERIAL -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: DRY RED MATERIAL -ANIMAL THIN -GI TRACT-LARGE LUMEN; INVOLVING ENTIRE GI TRACT: UP TO 18 MM IN DIAMETER; DISTENDED WITH GAS 1788 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 190 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90779 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 442.5 GRAMS DATE AND TIME OF NECROPSY: 11/03/99 14:02 PROSECTOR: JOHN S. HALFORD RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1789 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 191 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90780 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 574.7 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:32 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0459 .0080 % .0202 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0441 .0077 % .0194 WEIGHT TAKEN BRAIN (BR) 2.2694 .3949 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.2515 .3918 % .9921 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.3423 .4076 % 1.0321 WEIGHT TAKEN LIVER (LI) 17.4975 3.0446 % 7.7102 WEIGHT TAKEN LUNG (LU) 1.9971 .3475 % .8800 WEIGHT TAKEN SPLEEN (SP) .7631 .1328 % .3363 WEIGHT TAKEN LEFT TESTIS (TE0) 1.6424 .2858 % .7237 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.6309 .2838 % .7186 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0171 .0030 % .0075 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0109 .0019 % .0048 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; STOMACH, GL (ST) : STOMACH, GL (ST) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); MUCOSA: SINGLE >NOT REQUIRED TO BE EXAMINED FOR ANIMAL DARK RED AREA, 3 X 1 MM PROSTATE (PR) : PROSTATE (PR) : -LARGE; 25 X 15 X 15 MM >NOT REQUIRED TO BE EXAMINED FOR ANIMAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 28 X 15 X 3 >NOT REQUIRED TO BE EXAMINED FOR ANIMAL MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 1790 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 192 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90780 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 574.7 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:32 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 1791 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 193 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90781 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 741.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:45 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATELY SEVERE, DIFFUSE PANCREAS (PA) : -INFLAMMATION, VASCULAR,-MINIMAL, MULTI- FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATE, MULTI- FOCAL -MINERALIZATION,-SLIGHT, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, DARK RED, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL GRAY AND SOFT -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1792 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 194 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90781 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 741.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:45 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU) ------------------------------------------------------------------------------------------------------------------------------------ 1793 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 195 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90782 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 664.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:48 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LIVER (LI) : LIVER (LI) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -FOCUS, ALTERED HEPATOCELLULAR, >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; MULTIPLE DARK RED AREAS, UP TO 2 MM IN EOSINOPHILIC,-MODERATE, MULTI-FOCAL VERIFIED AT NECROPSY: NOT OBSERVED DIAMETER; COLLECTED ON ROUTINE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >MASS(ES); DORSAL; WEEK 105; VERIFIED SECTIONS FOCAL AT NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 13 X 7 X 4 -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE MM; BOTH APPEAR SOFT -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT SKIN (SK) : -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT 1794 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 196 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90782 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 664.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:48 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : -MASS(ES); DORSAL THORACIC REGION: SINGLE MASS, 10 MM IN DIAMETER, TAN, FIRM AND FRIABLE; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: -EROSION,-MODERATELY SEVERE, FOCAL SINGLE DARK RED AREA, 10 MM IN -EXOSTOSES,-MODERATELY SEVERE, FOCAL DIAMETER; SURROUNDING TISSUE APPEARS -FIBROSIS,-MODERATE, FOCAL SWOLLEN; COLLECTED -INFLAMMATION, ACUTE,-SLIGHT, FOCAL LN, POPLITEAL (PP) : LN, POPLITEAL (PP) : -LARGE; RIGHT: 12 X 8 X 7 MM (LEFT IS -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, NOT IDENTIFIED) (CAP 23) DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1795 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 197 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90782 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 664.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:48 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), URINARY BLADDER (UB), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 1796 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 198 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90783 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 657.2 GRAMS DATE AND TIME OF NECROPSY: 10/26/00 8:02 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- KIDNEY (KD) : LEFT; VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL, FOCAL URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; -HEMORRHAGE,-MODERATE, MULTI-FOCAL COLLECTED FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT -EDEMA,-MODERATE, FOCAL HINDFOOT: SINGLE DARK RED AND MOIST -EROSION,-MODERATELY SEVERE, FOCAL ABRADED AREA, 13 X 9 MM; APPEARS -EXOSTOSES,-MODERATE, FOCAL SWOLLEN; COLLECTED -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL LN, POPLITEAL (PP) : LN, POPLITEAL (PP) : -LARGE; LEFT: 10 X 8 X 4 MM (RIGHT IS >UNREMARKABLE 3 X 2 X 1 MM) (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1797 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 199 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90783 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 657.2 GRAMS DATE AND TIME OF NECROPSY: 10/26/00 8:02 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), TESTIS (TE), LUNG (LU) ------------------------------------------------------------------------------------------------------------------------------------ 1798 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 200 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90784 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 454.0 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:10 PROSECTOR: JARED FISHEL RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; SORE/SCAB; VENTRAL- -DILATATION, VENTRICULAR,-PRESENT THORACIC; VERIFIED AT NECROPSY: NOT KIDNEY (KD) : OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >APPEARANCE; THIN; VERIFIED AT FOCAL NECROPSY: OBSERVED SPLEEN (SP) : SPLEEN (SP) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -ROUGH SURFACE; DIFFUSE >UNREMARKABLE NECROPSY: NOT APPLICABLE ADRENAL, MEDULLA (MA) : >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT AT NECROPSY: NOT APPLICABLE THYROID (TY) : >EXCRETION; FEW FECES; VERIFIED AT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT NECROPSY: NOT APPLICABLE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL LOBULATED AND SOFT -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; RIGHT: 10 X 7 X 6 MM (LEFT IS -DILATATION, SINUSOIDAL,-SLIGHT, MULTI- 2 X 1 X 1 MM) FOCAL MAMMARY, MALE (MM) : -PIGMENT,-MINIMAL, MULTI-FOCAL 1799 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 201 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90784 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 454.0 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:10 PROSECTOR: JARED FISHEL RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: -EROSION,-SLIGHT, FOCAL SINGLE DRY, DARK RED CRUSTED AREA, 4 -FIBROSIS,-SLIGHT, FOCAL MM IN DIAMETER; COLLECTED -INFLAMMATION, ACUTE,-SLIGHT, FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 1800 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 202 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90784 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 454.0 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:10 PROSECTOR: JARED FISHEL RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1801 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 203 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90785 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 481.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:01 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 89; 55 X 65MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : LIVER (LI) : >APPEARANCE; HUNCHED; VERIFIED AT -MOTTLED; ALL LOBES: RED AND TAN -DEGENERATION, CYSTIC,-MODERATE, MULTI- NECROPSY: NOT APPLICABLE -RED FOCUS(I)/AREA(S); ALL LOBES: FOCAL >APPEARANCE; THIN; VERIFIED AT MULTIPLE DARK RED AREAS, UP TO 2 MM IN -TELANGIECTASIS,-SLIGHT, FOCAL NECROPSY: NOT OBSERVED DIAMETER; COLLECTED ON ROUTINE -VACUOLATION, HEPATOCELLULAR,- >EXCRETION; FEW FECES; VERIFIED AT SECTIONS MODERATELY SEVERE, MULTI-FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : KIDNEY (KD) : >SKIN & PELAGE; SORE/SCAB; PAW-HIND- -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- RIGHT; VERIFIED AT NECROPSY: OBSERVED SEVERE, DIFFUSE >WART LIKE LESION(S); TAIL; WEEK 104; PANCREAS (PA) : VERIFIED AT NECROPSY: OBSERVED -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-MINIMAL, MULTI- FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 7 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -EDEMA,-MODERATE, DIFFUSE STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE -EDEMA,-MODERATE, FOCAL 1802 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 204 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90785 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 481.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:01 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, NONGL (SU) : -INFLAMMATION, ACUTE,-MODERATE, FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL SKIN (SK) : -B-LIPOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); LEFT AXILLARY REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL SINGLE MASS, 45 X 35 X 25 MM, TAN AND >NOTE:>MAMMARY MASS DESCRIBED GROSSLY SOFT; SAME ON CUT SURFACE; COLLECTED; WAS A SUBCUTANEOUS LIPOMA. REGIONAL LN (SEE AXILLARY) LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; RIGHT: 10 X 6 X 3 MM (LEFT IS >UNREMARKABLE 3 X 2 X 1 MM) -MOTTLED; BILATERAL: RED AND TAN (CAP 22) TAIL (TI) : TAIL (TI) : -CRUSTED AREA(S); ENTIRE LENGTH: -EROSION,-SEVERE, FOCAL MULTIPLE DARK BROWN AND RAISED AREAS, -INFLAMMATION, ACUTE,-SEVERE, FOCAL UP TO 6 MM IN DIAMETER; PORTION COLLECTED FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: -EROSION,-MODERATELY SEVERE, FOCAL SINGLE DARK BROWN AREA, 5 MM IN -INFLAMMATION, ACUTE,-SEVERE, FOCAL DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN 1803 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 205 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90785 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 481.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:01 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 1804 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 206 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90786 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/02/99 STUDY DAY OF DEATH: 646 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/02/99 14:11 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -DEGENERATION, CYSTIC,-MODERATE, MULTI- FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL THYMUS (TH) : THYMUS (TH) : -RED FOCUS(I)/AREA(S); DIFFUSE: -HEMORRHAGE,-MINIMAL, MULTI-FOCAL MULTIPLE DARK RED FOCI, UP TO 1 MM IN -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO DIAMETER NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -MALIGNANT LYMPHOMA,-PRESENT 1805 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 207 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90786 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/02/99 STUDY DAY OF DEATH: 646 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/02/99 14:11 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) ------------------------------------------------------------------------------------------------------------------------------------ 1806 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 208 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90787 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 652.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 12:59 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -DEGENERATION, CYSTIC,-MODERATE, MULTI- MULTIPLE DARK RED FOCI, UP TO 2 MM IN FOCAL DIAMETER; COLLECTED ON ROUTINE -FOCUS, ALTERED HEPATOCELLULAR, SECTIONS EOSINOPHILIC,-SLIGHT, FOCAL -HEMORRHAGE,-MINIMAL, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL LUNG (LU) : -MINERALIZATION,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,- SLIGHT, DIFFUSE -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1807 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 209 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90787 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 652.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 12:59 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: UP TO 12 X 8 X 5 MM -DILATATION, SINUSOIDAL,-MODERATELY (CAP 22) SEVERE, MULTI-FOCAL JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; BILATERAL, TARSAL JOINTS: EACH -EDEMA,-MODERATELY SEVERE, MULTI-FOCAL IS UP TO 15 X 11 X 11 MM; BOTH -EXOSTOSES,-MODERATELY SEVERE, MULTI- COLLECTED FOCAL -FIBROSIS,-MODERATELY SEVERE, MULTI- FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1808 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 210 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90787 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 652.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 12:59 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 1809 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 211 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90788 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1042.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:04 PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -TELANGIECTASIS,-MINIMAL, FOCAL MULTIPLE DARK RED AREAS, UP TO 4 X 3 MM, COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -HEMORRHAGE,-MODERATE, MULTI-FOCAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 1810 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 212 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90788 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1042.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:04 PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 1811 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 213 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90789 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 407.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:37 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 12.0305 2.9501 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1812 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 214 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90789 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 407.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:37 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 1813 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 215 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90790 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 637.6 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 12:45 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >BEHAVIOR; HYPOACTIVE; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED -DILATATION, VENTRICULAR,-PRESENT AT NECROPSY: NOT APPLICABLE LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -DEGENERATION, CYSTIC,-SLIGHT, FOCAL NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, >SKIN & PELAGE; BROWN HAIR COAT; EOSINOPHILIC,-SLIGHT, FOCAL VENTRAL-ABDOMINAL; VERIFIED AT -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NECROPSY: OBSERVED FOCAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 7 X 12 MM, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SOFT, RED, DARK RED AND TAN -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-MODERATE, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1814 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 216 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90790 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 637.6 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 12:45 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: MOIST YELLOW MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1815 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 217 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90791 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 757.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:28 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -FOCUS, ALTERED HEPATOCELLULAR, CLEAR MULTIPLE DARK RED AREAS, UP TO 2 MM IN CELL,-MODERATELY SEVERE, FOCAL DIAMETER; COLLECTED ON ROUTINE -FOCUS, ALTERED HEPATOCELLULAR, SECTIONS EOSINOPHILIC,-SLIGHT, MULTI-FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, MULTI-FOCAL URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1816 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 218 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90791 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 757.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:28 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 1817 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 219 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90792 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/99 STUDY DAY OF DEATH: 396 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: 474.7 GRAMS DATE AND TIME OF NECROPSY: 02/25/99 12:04 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; SQUINTED; PERI-ORBITAL; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT OBSERVED TESTIS (TE) : >EXCRETION; NO FECES; VERIFIED AT -MINERALIZATION, VASCULAR,-MINIMAL, NECROPSY: NOT APPLICABLE FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT LUNG (LU) : NECROPSY: NOT APPLICABLE -FIBROSIS, PLEURAL/SUBPLEURAL,-SLIGHT, >RESPIRATION; IRREGULAR; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 8 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- TAN, GRAY AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -EDEMA,-MODERATE, FOCAL ULCERATED AREAS, 1 MM IN DIAMETER -EROSION,-MINIMAL, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL ILEUM (IL) : ILEUM (IL) : >NOTE:>TRIMMING COMMENT: MISSING AT >TISSUE MISSING TIME OF TRIM CECUM (CE) : >TISSUE MISSING 1818 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 220 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90792 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/99 STUDY DAY OF DEATH: 396 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: 474.7 GRAMS DATE AND TIME OF NECROPSY: 02/25/99 12:04 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CECUM (CE) : >NOTE:>TRIMMING COMMENT: MISSING AT TIME OF TRIM COLON (CO) : COLON (CO) : >NOTE:>TRIMMING COMMENT: MISSING AT >TISSUE MISSING TIME OF TRIM PROSTATE (PR) : -EDEMA,-SLIGHT, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 1819 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 221 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90792 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/99 STUDY DAY OF DEATH: 396 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: 474.7 GRAMS DATE AND TIME OF NECROPSY: 02/25/99 12:04 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1820 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 222 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90793 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/19/99 12:18 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MODERATE, MULTI- FOCAL ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MODERATE, FOCAL -VACUOLATION,-SLIGHT, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 7 MM, RED, DARK -HEMORRHAGE,-SLIGHT, MULTI-FOCAL RED, GRAY AND SOFT -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, FOCAL -------------------------------------------- 1821 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 223 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90793 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/19/99 12:18 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1822 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 224 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90794 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 759.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:33 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LIVER (LI) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >SKIN & PELAGE; BROWN HAIR COAT; EOSINOPHILIC,-MINIMAL, FOCAL VENTRAL-ABDOMINAL; VERIFIED AT -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NECROPSY: OBSERVED FOCAL KIDNEY (KD) : -INFARCT,-SLIGHT, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL -PYELONEPHRITIS,-MINIMAL, MULTI-FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -LARGE; UP TO 5 MM IN DIAMETER (CAP >TISSUE MISSING 22) >NOTE:>PANCREATIC LYMPH NODE DESCRIBED -DIFFUSELY RED; DARK RED GROSSLY WAS A ISLET CELL ADENOMA OF THE PANCREAS LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: UP TO 19 X 11 X 10 -DILATATION, SINUSOIDAL,-MODERATELY MM (CAP 23) SEVERE, MULTI-FOCAL 1823 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 225 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90794 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 759.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:33 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: -EROSION,-MODERATELY SEVERE, MULTI- SINGLE DARK RED AND TAN AREA, 10 X 9 FOCAL MM; APPEARS SWOLLEN; COLLECTED -EXOSTOSES,-MODERATE, MULTI-FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BROWN AND DRY -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 1824 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 226 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90794 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 759.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:33 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 1825 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 227 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90795 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/01/99 STUDY DAY OF DEATH: 400 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 431.6 GRAMS DATE AND TIME OF NECROPSY: 03/01/99 13:40 PROSECTOR: TERRI L. WILLIAMS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT EYE (EY) : NECROPSY: NOT APPLICABLE -INFLAMMATION, ACUTE, CILIARY,-SLIGHT, >APPEARANCE; THIN; VERIFIED AT MULTI-FOCAL NECROPSY: NOT OBSERVED BRAIN (BR) : BRAIN (BR) : >RESPIRATION; AUDIBLE; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; ROUGH HAIR COAT; KIDNEY (KD) : VERIFIED AT NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MINIMAL, MULTI- >SKIN & PELAGE; BROWN HAIR COAT; FOCAL GENERALIZED; VERIFIED AT NECROPSY: NOT ADRENAL, CORTEX (AC) : OBSERVED -DEGENERATION, CYSTIC, CORTICAL,- >DISCHARGE; NASAL; RED IN COLOR; MINIMAL, FOCAL VERIFIED AT NECROPSY: OBSERVED -HEMORRHAGE,-MINIMAL, FOCAL >EYES; RED DISCHARGE; EYES; VERIFIED AT PITUITARY (PI) : PITUITARY (PI) : NECROPSY: OBSERVED -MASS(ES); 12 X 10 X 8 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, GRAY AND SOFT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; MUCOSA, DIFFUSE -EDEMA,-MODERATELY SEVERE, MULTI-FOCAL -EROSION,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL RECTUM (RE) : RECTUM (RE) : >NOTE:>TRIMMING COMMENT: MISSING AT >TISSUE MISSING TRIMMING 1826 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 228 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90795 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/01/99 STUDY DAY OF DEATH: 400 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 431.6 GRAMS DATE AND TIME OF NECROPSY: 03/01/99 13:40 PROSECTOR: TERRI L. WILLIAMS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); BILATERAL AXILLARY REGIONS: -GALACTOCELE,-SLIGHT, MULTI-FOCAL MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER (CAP 21) PENIS (PE) : PENIS (PE) : -PROTRUDING; APPROXIMATELY 9 MM -PROLAPSE,-PRESENT EXPOSED; COLLECTED PREPUTIAL GLAND (PG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL >NOTE:>PREPUTIAL GLAND WAS PRESENT ON PENIS SLIDE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -DISCHARGE-NASAL; BILATERAL: DRY RED MATERIAL -------------------------------------------- 1827 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 229 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90795 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/01/99 STUDY DAY OF DEATH: 400 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 431.6 GRAMS DATE AND TIME OF NECROPSY: 03/01/99 13:40 PROSECTOR: TERRI L. WILLIAMS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1828 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 230 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90796 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/13/99 STUDY DAY OF DEATH: 412 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/13/99 16:23 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 8 X 6 MM, RED, TAN, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL DARK RED AND FIRM -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK BROWN AREAS, UP TO 3 X 2 MM STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE -EDEMA,-SLIGHT, FOCAL DARK BROWN AREA, 2 X 1 MM -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL JEJUNUM (JE) : >AUTOLYTIC PROSTATE (PR) : PROSTATE (PR) : -NODULAR; TISSUE PROXIMAL TO URETHRA: -INFLAMMATION, CHRONIC-ACTIVE,- GREEN AND FIRM MODERATE, MULTI-FOCAL 1829 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 231 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90796 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/13/99 STUDY DAY OF DEATH: 412 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/13/99 16:23 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -PIGMENT,-MINIMAL, MULTI-FOCAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1830 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 232 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90797 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 533.4 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:29 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0263 .0049 % .0117 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0244 .0046 % .0108 WEIGHT TAKEN BRAIN (BR) 2.2572 .4232 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.7313 .3246 % .7670 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.7203 .3225 % .7621 WEIGHT TAKEN LIVER (LI) 13.8052 2.5882 % 6.1161 WEIGHT TAKEN LUNG (LU) 1.7917 .3359 % .7938 WEIGHT TAKEN SPLEEN (SP) .8018 .1503 % .3552 WEIGHT TAKEN LEFT TESTIS (TE0) 1.7172 .3219 % .7608 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.8086 .3391 % .8013 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0090 .0017 % .0040 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0183 .0034 % .0081 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR,-SLIGHT, FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: TWO >NOT REQUIRED TO BE EXAMINED FOR ANIMAL PINPOINT FOCI CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 1831 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 233 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90797 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 533.4 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:29 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 1832 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 234 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90798 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 703 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 746.3 GRAMS DATE AND TIME OF NECROPSY: 12/29/99 14:36 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT EYE (EY) : NECROPSY: OBSERVED -VASCULARIZATION, CORNEAL,-MINIMAL, >RESPIRATION; AUDIBLE; VERIFIED AT MULTI-FOCAL NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >RESPIRATION; LABORED; VERIFIED AT -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC -FIBROSIS, CAPSULAR,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE SURFACE OF LEFT LATERAL LOBE: SINGLE FOCAL >EXCRETION; FEW FECES; VERIFIED AT TAN AND LINEAR AREA, 7 X 2 MM; -FOCUS, ALTERED HEPATOCELLULAR, NECROPSY: NOT APPLICABLE COLLECTED ON ROUTINE SECTION EOSINOPHILIC,-SEVERE, FOCAL -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- SURFACE OF LEFT LATERAL LOBE: SINGLE FOCAL DARK RED AREA, 4 MM IN DIAMETER; -NECROSIS, HEPATOCELLULAR,-MINIMAL, COLLECTED ON ROUTINE SECTION MULTI-FOCAL -TELANGIECTASIS,-MODERATE, FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, MULTI-FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 1833 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 235 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90798 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 703 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 746.3 GRAMS DATE AND TIME OF NECROPSY: 12/29/99 14:36 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -HYPERPLASIA, C-CELL,-MINIMAL, FOCAL PITUITARY (PI) : -CYST,-PRESENT -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT TAIL (TI) : TAIL (TI) : -CRUSTED AREA(S); ALONG ENTIRE LENGTH: -CYST, EPIDERMAL INCLUSION,-PRESENT MULTIPLE TAN, DRY, AND SLIGHTLY RAISED -EROSION,-MODERATE, FOCAL AREAS, UP TO 3 MM IN DIAMETER; -INFLAMMATION, ACUTE,-MODERATELY COLLECTED SEVERE, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN OFF AT BASE -------------------------------------------- 1834 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 236 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90798 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 703 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 746.3 GRAMS DATE AND TIME OF NECROPSY: 12/29/99 14:36 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1835 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 237 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90799 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 685.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:17 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NONFORMED FECES; VERIFIED LIVER (LI) : LIVER (LI) : AT NECROPSY: NOT APPLICABLE -MASS(ES); VISCERAL SURFACE OF LEFT -FOCUS, ALTERED HEPATOCELLULAR, LATERAL LOBE: SINGLE MASS, 15 X 10 X 8 EOSINOPHILIC,-MINIMAL, MULTI-FOCAL MM, RED, TAN AND SOFT; SAME ON CUT -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- SURFACE; COLLECTED ON ROUTINE SECTION; FOCAL REGIONAL LN (HEPATIC) NOT IDENTIFIED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL PANCREAS (PA) : -HYPERPLASIA, ACINAR,-SLIGHT, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN 1836 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 238 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90799 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 685.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:17 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU) ------------------------------------------------------------------------------------------------------------------------------------ 1837 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 239 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90800 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/26/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/26/99 11:21 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NONFORMED FECES; VERIFIED LIVER (LI) : AT NECROPSY: NOT APPLICABLE -DEGENERATION, CYSTIC,-MINIMAL, FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : KIDNEY (KD) : -CALCULUS(I); RIGHT, WITHIN PELVIS: -CAST, PROTEINACEOUS,-MINIMAL, FOCAL SINGLE TAN, HARD AND FLAT CALCULUS, 4 -MINERALIZATION, PELVIC,-SLIGHT, MULTI- X 2 X LESS THAN 1 MM FOCAL -LARGE; BILATERAL: EACH IS 30 X 20 X 16 MM ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 13 X 8 X 8 MM (LEFT IS -HYPERTROPHY, CORTICAL CELL,-MODERATE, 5 X 4 X 4 MM) FOCAL -VACUOLATION,-MODERATE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LUNG (LU) : LUNG (LU) : -DIFFUSELY RED; ALL LOBES: DARK RED >UNREMARKABLE HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI- FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL 1838 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 240 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90800 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/26/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/26/99 11:21 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -HEPATOCELLULAR ADENOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL: DARK BROWN AND -DILATATION, SINUSOIDAL,-MODERATE, TAN (CAP 21) MULTI-FOCAL -------------------------------------------- 1839 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 241 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90800 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/26/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/26/99 11:21 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1840 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 242 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90801 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/11/99 STUDY DAY OF DEATH: 685 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/11/99 9:35 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); LIMB-HIND-RIGHT; WEEK 98; 40 BRAIN (BR) : X 40MM; VERIFIED AT NECROPSY: OBSERVED -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO MULTIPLE TAN AREAS, UP TO 15 MM IN NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT DIAMETER (CAP 22) -MOTTLED; ALL LOBES: RED, DARK RED, DARK BROWN AND TAN KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL PANCREAS (PA) : -HEMORRHAGE,-MODERATELY SEVERE, FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT LN, MESENTERIC (MS) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI- FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 5 X 5 X 4 MM; APPEARS SWOLLEN -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 1841 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 243 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90801 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/11/99 STUDY DAY OF DEATH: 685 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/11/99 9:35 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC SKIN (SK) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -HISTIOCYTIC SARCOMA,-PRESENT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -LARGE; ANTERIOR MESENTERIC: 7 MM IN -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO DIAMETER (CAP 23) NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); RIGHT LATERAL ABDOMINAL >TISSUE MISSING REGION, EXTENDING TO RIGHT LATERAL >NOTE:>MAMMARY MASS DESCRIBED GROSSLY HINDLEG: SINGLE MASS, 45 X 30 X 20 MM, WAS HISTIOCYTIC SARCOMA IN THE RED, TAN AND FIRM; SAME ON CUT SKIN SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED -------------------------------------------- 1842 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 244 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90801 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/11/99 STUDY DAY OF DEATH: 685 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/11/99 9:35 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) ------------------------------------------------------------------------------------------------------------------------------------ 1843 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 245 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90802 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/10/99 STUDY DAY OF DEATH: 562 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 435.8 GRAMS DATE AND TIME OF NECROPSY: 08/10/99 13:58 PROSECTOR: DENNIS HOFFMAN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >SKIN & PELAGE; RED HAIR COAT; HEAD- -VACUOLATION, HEPATOCELLULAR,-MODERATE, ENTIRE; VERIFIED AT NECROPSY: OBSERVED MULTI-FOCAL >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >SKIN & PELAGE; COLD TO TOUCH; ENTIRE FOCAL BODY; VERIFIED AT NECROPSY: NOT -MINERALIZATION, PELVIC,-MODERATE, APPLICABLE MULTI-FOCAL >RESPIRATION; LABORED; VERIFIED AT PANCREAS (PA) : NECROPSY: NOT APPLICABLE -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, FOCAL BONE, FEMUR (FE) : BONE, FEMUR (FE) : >NOTE:>TRIMMING COMMENT: NOT FOUND AT >TISSUE MISSING TRIMMING MARROW, FEMUR (FM) : MARROW, FEMUR (FM) : >NOTE:>TRIMMING COMMENT: NOT FOUND AT >TISSUE MISSING TRIMMING THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 1844 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 246 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90802 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/10/99 STUDY DAY OF DEATH: 562 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 435.8 GRAMS DATE AND TIME OF NECROPSY: 08/10/99 13:58 PROSECTOR: DENNIS HOFFMAN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 6 X8 MM, SOFT, RED, AND DARK RED STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -EDEMA,-SLIGHT, MULTI-FOCAL TAN AND RED ERODED AREAS, UP TO 2 X 3 -EROSION,-MODERATE, FOCAL MM -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, -THICKENED WALL; DIFFUSE MULTI-FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -DISCHARGE-NASAL; DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 1845 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 247 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90802 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/10/99 STUDY DAY OF DEATH: 562 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 435.8 GRAMS DATE AND TIME OF NECROPSY: 08/10/99 13:58 PROSECTOR: DENNIS HOFFMAN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1846 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 248 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90803 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/07/99 STUDY DAY OF DEATH: 651 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/07/99 8:00 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; OPAQUE; EYES; VERIFIED AT EYE (EY) : EYE (EY) : NECROPSY: OBSERVED -OPAQUE CORNEA; BILATERAL -FIBROSIS, LENS,-MINIMAL, FOCAL >WART LIKE LESION(S); TAIL; WEEK 93; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LARGE; ALL LOBES >UNREMARKABLE KIDNEY (KD) : KIDNEY (KD) : -LARGE; BILATERAL: EACH IS 29 X 21 X -NEPHROPATHY, CHRONIC PROGRESSIVE,- 19 MM MINIMAL, MULTI-FOCAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 12 X 7 X 6 -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE MM LUNG (LU) : LUNG (LU) : -UNCOLLAPSED; ALL LOBES -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MODERATE, MULTI- FOCAL -FIBROSIS,-MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, FOCAL -CYST, ULTIMOBRANCHIAL,-PRESENT PARATHYROID (PT) : -B-ADENOMA,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, FOCAL -B-ADENOMA,-NEOPLASM PRESENT 1847 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 249 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90803 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/07/99 STUDY DAY OF DEATH: 651 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/07/99 8:00 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 9 X 5 >UNREMARKABLE MM CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT TAIL (TI) : TAIL (TI) : -RAISED AREA(S); 41 MM FROM BASE: -CYST, EPIDERMAL INCLUSION,-PRESENT SINGLE DRY, TAN RAISED AREA, 3 X 3 X 2 MM; COLLECTED -------------------------------------------- 1848 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 250 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90803 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/07/99 STUDY DAY OF DEATH: 651 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/07/99 8:00 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1849 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 251 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90804 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 501.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:35 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: AS ASSIGNED ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; DORSAL; EYE (EY) : EYE (EY) : VERIFIED AT NECROPSY: OBSERVED -OPAQUE CORNEA; BILATERAL -INFLAMMATION, ACUTE, CILIARY,-SLIGHT, >EYES; OPAQUE; EYES; VERIFIED AT MULTI-FOCAL NECROPSY: OBSERVED -INFLAMMATION, ACUTE, CORNEAL,- >EXCRETION; FEW FECES; VERIFIED AT MODERATE, MULTI-FOCAL NECROPSY: NOT APPLICABLE -ULCERATION, CORNEAL,-MODERATE, MULTI- >APPEARANCE; THIN; VERIFIED AT FOCAL NECROPSY: NOT OBSERVED -VASCULARIZATION, CORNEAL,-MODERATE, >APPEARANCE; HUNCHED; VERIFIED AT MULTI-FOCAL NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -DEGENERATION, CYSTIC,-SLIGHT, MULTI- MULTIPLE DARK RED AND SLIGHTLY FOCAL DEPRESSED AREAS, UP TO 3 MM IN -FOCUS, ALTERED HEPATOCELLULAR, DIAMETER; COLLECTED ON ROUTINE EOSINOPHILIC,-MODERATE, FOCAL SECTIONS -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL -TELANGIECTASIS,-MINIMAL, FOCAL KIDNEY (KD) : KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -CYST,-SEVERE, FOCAL -DIFFUSELY LIGHT; BILATERAL: TAN -NEPHROPATHY, CHRONIC PROGRESSIVE,- -CYST(S); BILATERAL, DIFFUSE: MULTIPLE SEVERE, DIFFUSE CLEAR FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER PARATHYROID (PT) : -HYPERPLASIA,-MODERATE, DIFFUSE 1850 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 252 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90804 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 501.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:35 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: AS ASSIGNED ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PARATHYROID (PT) : -LARGE; BILATERAL: EACH IS 2 X 2 X 1 MM STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -EDEMA,-MODERATE, FOCAL -MINERALIZATION,-MODERATE, MULTI-FOCAL SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); DORSAL THORACIC -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM REGION: SINGLE DARK RED AND DRY AREA, PRESENT 4 MM IN DIAMETER (CAP 22) URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1851 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 253 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90804 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 501.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:35 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: AS ASSIGNED ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), LUNG (LU), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 1852 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 254 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90805 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/24/99 STUDY DAY OF DEATH: 423 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 457.0 GRAMS DATE AND TIME OF NECROPSY: 03/24/99 7:58 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; SQUINTED; PERI-ORBITAL; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >SKIN & PELAGE; BROWN HAIR COAT; -CAST, PROTEINACEOUS,-MINIMAL, MULTI- SCROTUM; VERIFIED AT NECROPSY: NOT FOCAL OBSERVED PANCREAS (PA) : >APPEARANCE; THIN; VERIFIED AT -PIGMENT,-MINIMAL, MULTI-FOCAL NECROPSY: NOT OBSERVED ADRENAL, MEDULLA (MA) : >APPEARANCE; HUNCHED; VERIFIED AT -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT NECROPSY: NOT APPLICABLE TESTIS (TE) : TESTIS (TE) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -SMALL; BILATERAL: EACH IS 15 X 8 X 4 -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE NECROPSY: NOT APPLICABLE MM; BOTH ARE SOFT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- TAN AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-SLIGHT, FOCAL -EROSION,-SLIGHT, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL 1853 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 255 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90805 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/24/99 STUDY DAY OF DEATH: 423 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 457.0 GRAMS DATE AND TIME OF NECROPSY: 03/24/99 7:58 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 10 X 5 X 2 >UNREMARKABLE MM EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 1854 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 256 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90805 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/24/99 STUDY DAY OF DEATH: 423 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 457.0 GRAMS DATE AND TIME OF NECROPSY: 03/24/99 7:58 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1855 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 257 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90806 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/98 STUDY DAY OF DEATH: 239 STUDY WEEK OF DEATH: 35 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/21/98 14:20 PROSECTOR: JAMES T. CALLEN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK LIVER (LI) : LIVER (LI) : 21; 95 X 100MM; VERIFIED AT NECROPSY: -DIFFUSELY LIGHT; ALL LOBES: LIGHT TAN >UNREMARKABLE OBSERVED LN, MESENTERIC (MS) : >SKIN & PELAGE; SORE/SCAB; VENTRAL- -HEMORRHAGE,-MINIMAL, MULTI-FOCAL ABDOMINAL-LEFT; VERIFIED AT NECROPSY: LUNG (LU) : LUNG (LU) : OBSERVED -ADHESION(S); TRIM COMMENT: LEFT LOBE -FIBROSIS, PLEURAL/SUBPLEURAL,- >APPEARANCE; THIN; VERIFIED AT ADHERED TO DIAPHRAGM: SINGLE TAN AND MODERATE, MULTI-FOCAL NECROPSY: OBSERVED FIBROUS ADHESION, 8 MM IN LENGTH >SKIN & PELAGE; PALE; ENTIRE BODY; THYROID (TY) : VERIFIED AT NECROPSY: NOT APPLICABLE -CYST, FOLLICULAR,-SLIGHT, MULTI-FOCAL SKIN (SK) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -NECROSIS,-SEVERE, MULTI-FOCAL -M-SARCOMA, UNDIFFERENTIATED,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -UNDIFFERENTIATED SARCOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; RIGHT, LUMBAR: 7 X 3 X 1 MM >UNREMARKABLE (LEFT NOT IDENTIFIED) (CAP 21) MAMMARY, MALE (MM) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>MAMMARY GLAND MASS DESCRIBED GROSSLY WAS A SUBCUTANEOUS FIBROMA 1856 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 258 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90806 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/98 STUDY DAY OF DEATH: 239 STUDY WEEK OF DEATH: 35 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/21/98 14:20 PROSECTOR: JAMES T. CALLEN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, MALE (MM) : -MASS(ES); EXTENDING FROM RIGHT TO LEFT INGUINAL REGION AND XIPHOID PROCESS TO PERIANAL REGION: SINGLE MASS, 120 X 110 X 60 MM (340.4 GRAMS), RED, TAN, DARK RED, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED OVERLYING SKIN HAS A SINGLE RED, DARK RED, CRUSTED AREA, 80 X 60 MM; COLLECTED INTACT WITH MASS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 1857 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 259 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90806 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/98 STUDY DAY OF DEATH: 239 STUDY WEEK OF DEATH: 35 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/21/98 14:20 PROSECTOR: JAMES T. CALLEN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1858 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 260 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90807 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 522.0 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:41 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0231 .0044 % .0104 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0207 .0040 % .0093 WEIGHT TAKEN BRAIN (BR) 2.2198 .4252 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.8047 .3457 % .8130 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.9042 .3648 % .8578 WEIGHT TAKEN LIVER (LI) 14.8853 2.8516 % 6.7057 WEIGHT TAKEN LUNG (LU) 1.7490 .3351 % .7879 WEIGHT TAKEN SPLEEN (SP) .8167 .1565 % .3679 WEIGHT TAKEN LEFT TESTIS (TE0) 1.8401 .3525 % .8289 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.8383 .3522 % .8281 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0109 .0021 % .0049 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0155 .0030 % .0070 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 1859 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 261 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90807 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 522.0 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:41 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 1860 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 262 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90808 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/31/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 398.6 GRAMS DATE AND TIME OF NECROPSY: 05/31/99 10:14 PROSECTOR: JOHN S. HALFORD RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >SKIN & PELAGE; ROUGH HAIR COAT; -VACUOLATION, HEPATOCELLULAR,-MINIMAL, VERIFIED AT NECROPSY: NOT APPLICABLE MULTI-FOCAL >APPEARANCE; HUNCHED; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: NOT APPLICABLE -MOTTLED; BILATERAL: DARK BROWN AND -CYST,-MINIMAL, FOCAL >EXCRETION; NO FECES; VERIFIED AT TAN -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE SLIGHT, MULTI-FOCAL >RESPIRATION; LABORED; VERIFIED AT PANCREAS (PA) : NECROPSY: NOT APPLICABLE -PIGMENT,-MINIMAL, MULTI-FOCAL >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED LN, MESENTERIC (MS) : AT NECROPSY: NOT APPLICABLE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 9 X 8 MM, RED, DARK -HEMORRHAGE,-SEVERE, MULTI-FOCAL RED, GRAY, TAN, LOBULATED, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE >UNREMARKABLE DARK BROWN AREA, 3 X 2 MM 1861 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 263 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90808 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/31/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 398.6 GRAMS DATE AND TIME OF NECROPSY: 05/31/99 10:14 PROSECTOR: JOHN S. HALFORD RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: CLEAR AND SOFT >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL, LUMBAR LN: EACH IS >UNREMARKABLE 6 X 4 X 3 MM (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 1862 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 264 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90808 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/31/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 398.6 GRAMS DATE AND TIME OF NECROPSY: 05/31/99 10:14 PROSECTOR: JOHN S. HALFORD RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1863 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 265 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90809 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 717.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 9:45 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: NOT OBSERVED -MOTTLED; ALL LOBES: RED AND TAN -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL TESTIS (TE) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -INFLAMMATION, GRANULOMATOUS,-SLIGHT, FOCAL URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED >UNREMARKABLE (CAP 22) LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; RIGHT: 6 MM IN DIAMETER (LEFT -DILATATION, SINUSOIDAL,-MODERATE, NOT IDENTIFIED); RIGHT APPEARS CYSTIC FOCAL (CAP 23) 1864 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 266 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90809 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 717.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 9:45 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU) ------------------------------------------------------------------------------------------------------------------------------------ 1865 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 267 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90810 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 910.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:45 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -DEGENERATION, CYSTIC,-MODERATELY MULTIPLE DARK RED AREAS, UP TO 3 MM IN SEVERE, MULTI-FOCAL DIAMETER; COLLECTED ON ROUTINE -FOCUS, ALTERED HEPATOCELLULAR, SECTIONS EOSINOPHILIC,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -NECROSIS, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -TELANGIECTASIS,-MODERATE, FOCAL KIDNEY (KD) : KIDNEY (KD) : -DARK FOCUS(I)/AREA(S); RIGHT CORTEX: -CYST,-MODERATE, FOCAL SINGLE BLACK AREA, 4 MM IN DIAMETER -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL PANCREAS (PA) : -HYPERPLASIA, ACINAR,-MINIMAL, MULTI- FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -DARK FOCUS(I)/AREA(S); RIGHT: SINGLE -DEGENERATION, CYSTIC, CORTICAL,- DARK BROWN AREA, 2 MM IN DIAMETER MODERATELY SEVERE, FOCAL 1866 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 268 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90810 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 910.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:45 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, FOCAL -THROMBUS,-MODERATE, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); POSTERIOR -B-ADENOMA,-NEOPLASM PRESENT ASPECT: SINGLE DARK RED AREA, 1 MM IN DIAMETER -LARGE; 6 X 5 X 4 MM; APPEARS SWOLLEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1867 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 269 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90810 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 910.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:45 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 1868 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 270 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90811 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 842.5 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:43 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); AT JUNCTION -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- OF RIGHT AND LEFT MEDIAN LOBES: SINGLE FOCAL YELLOW FOCUS, 1 MM IN DIAMETER; -LIPIDOSIS, SUBCAPSULAR (TENSION COLLECTED LIPIDOSIS),-SLIGHT, FOCAL -RED FOCUS(I)/AREA(S); ALL LOBES, -VACUOLATION, HEPATOCELLULAR,-MINIMAL, DIFFUSE: MULTIPLE DARK RED AREAS, UP MULTI-FOCAL TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -INFARCT,-SLIGHT, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL PANCREAS (PA) : -CYST,-MINIMAL, FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL TESTIS (TE) : -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT 1869 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 271 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90811 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 842.5 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:43 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE >UNREMARKABLE URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; -HEMORRHAGE,-SLIGHT, MULTI-FOCAL COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 1870 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 272 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90812 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 502.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:03 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; YELLOW HAIR COAT; -DILATATION, VENTRICULAR,-PRESENT VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; BROWN HAIR COAT; -RED FOCUS(I)/AREA(S); ALL LOBES: -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- PERINEAL AREA; VERIFIED AT NECROPSY: MULTIPLE DARK RED AREAS, UP TO 5 MM IN FOCAL OBSERVED DIAMETER; SOME ARE SUNKEN WITH A -PIGMENT,-SLIGHT, FOCAL >APPEARANCE; HUNCHED; VERIFIED AT STELLATE PATTERN; COLLECTED ON ROUTINE -TELANGIECTASIS,-MODERATE, FOCAL NECROPSY: NOT APPLICABLE SECTIONS -THROMBUS,-SLIGHT, FOCAL >DISCHARGE; NASAL; RED IN COLOR; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL MUSCLE, SKELETAL (SM) : MUSCLE, SKELETAL (SM) : -ATROPHY-FOCAL; BILATERAL HIND LEGS; -DEGENERATION/NECROSIS,-MINIMAL, MULTI- COLLECTED ON ROUTINE SECTION FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 1871 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 273 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90812 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 502.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:03 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, RED, GRAY, TAN AND SOFT SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); LEFT LATERAL -B-KERATOACANTHOMA,-NEOPLASM PRESENT THORACIC REGION: SINGLE TAN, DRY, LOBULATED AND CRUSTED AREA, 5 MM IN DIAMETER (CAP 22) PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, URETHRA: GREEN AND FIRM MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT ADIPOSE TISSUE (AT) : ADIPOSE TISSUE (AT) : -MASS(ES); VENTRAL CERVICAL REGION: -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- SINGLE MASS, 25 X 15 X 10 MM, WHITE, FOCAL SOFT AND GELATINOUS; SAME ON CUT -B-LIPOMA,-NEOPLASM PRESENT SURFACE (CAP 23); REGIONAL LN (MANDIBULAR) NORMAL (CAP 24) GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; PERINEUM: YELLOWISH-BROWN AND MOIST -BLOOD FILM TAKEN -DISCHARGE-NASAL; DARK RED AND DRY -STAINS - SKIN/HAIRCOAT; ABDOMINAL REGION: YELLOW AND DRY -BONE MARROW SMEAR TAKEN -------------------------------------------- 1872 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 274 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90812 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 502.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:03 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), URINARY BLADDER (UB), LN, MANDIBULAR (MN) ------------------------------------------------------------------------------------------------------------------------------------ 1873 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 275 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90813 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/18/98 STUDY DAY OF DEATH: 52 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 339.5 GRAMS DATE AND TIME OF NECROPSY: 03/18/98 14:18 PROSECTOR: NANCY DIEDRICH RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >DISCHARGE; ORAL; RED IN COLOR; LUNG (LU) : LUNG (LU) : VERIFIED AT NECROPSY: OBSERVED -DIFFUSELY RED; CRANIAL REGIONS OF ALL >UNREMARKABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT LOBES: DARK RED NECROPSY: OBSERVED HEART (HT) : >SKIN & PELAGE; RED HAIR COAT; VENTRAL- -DEGENERATION/NECROSIS,-MINIMAL, MULTI- ABDOMINAL; VERIFIED AT NECROPSY: FOCAL OBSERVED CAUSE OF DEATH (CD) : >APPEARANCE; THIN; VERIFIED AT -FRACTURE, HARD PALATE,-PRESENT NECROPSY: OBSERVED CAVITY, ORAL (OC) : CAVITY, ORAL (OC) : >EXCRETION; FEW FECES; VERIFIED AT -FRACTURE(S); EXTENDING FROM HARD -BACTERIAL COLONIES,-PRESENT NECROPSY: NOT APPLICABLE PALATE TO NASAL BONE: SINGLE FRACTURE, -FRACTURE,-PRESENT >RESPIRATION; IRREGULAR; VERIFIED AT 5 MM IN WIDTH; SURROUNDING TISSUE DARK -HEMORRHAGE,-MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE RED; COLLECTED -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL GENERAL COMMENT (GC) : -DISCHARGE-ORAL; DARK RED AND CRUSTED -STAINS-PERINEUM/PERIANAL; DARK RED AND DRY -BLOOD FILM TAKEN -DISCHARGE-OCULAR; DARK RED AND CRUSTED -MALOCCLUSION; BILATERAL: UPPER INCISORS -STAINS - SKIN/HAIRCOAT; ABDOMEN: DARK RED AND DRY 1874 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 276 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90813 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/18/98 STUDY DAY OF DEATH: 52 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 339.5 GRAMS DATE AND TIME OF NECROPSY: 03/18/98 14:18 PROSECTOR: NANCY DIEDRICH RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1875 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 277 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90814 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 323.5 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:13 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 9.3873 2.9018 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1876 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 278 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90814 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 323.5 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:13 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 1877 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 279 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90815 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 638.5 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:18 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); DORSAL; WEEK 76; LIVER (LI) : 15 X 15MM; VERIFIED AT NECROPSY: -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- OBSERVED FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL SPLEEN (SP) : SPLEEN (SP) : -LARGE; 50 X 10 X 5 MM >UNREMARKABLE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL SKIN (SK) : SKIN (SK) : -MASS(ES); DORSAL THORACIC REGION: -INFLAMMATION, ACUTE,-MODERATE, FOCAL SINGLE MASS, 25 X 20 X 10 MM, RED, TAN -ULCERATION,-MODERATELY SEVERE, FOCAL AND GELATINOUS; SAME ON CUT SURFACE; -M-CARCINOMA, SQUAMOUS CELL,-NEOPLASM OVERLYING SURFACE IS DARK RED AND PRESENT ABRADED; COLLECTED; REGIONAL LN (SEE AXILLARY) URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1878 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 280 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90815 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 638.5 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:18 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; RIGHT: UP TO 12 X 6 X 4 MM -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, (LEFT IS UP TO 5 X 3 X 2 MM) (CAP 22) MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), THYROID (TY), PARATHYROID (PT) ------------------------------------------------------------------------------------------------------------------------------------ 1879 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 281 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90816 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/15/99 STUDY DAY OF DEATH: 598 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 565.0 GRAMS DATE AND TIME OF NECROPSY: 09/15/99 14:14 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; ROUGH HAIR COAT; -DILATATION, VENTRICULAR,-PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -HEMORRHAGE,-MINIMAL, FOCAL NECROPSY: NOT APPLICABLE -NECROSIS, HEPATOCELLULAR,-MODERATE, >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED FOCAL AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -CAST, PROTEINACEOUS,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 8 X 8 MM, RED, GRAY, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,- URETHRA: GREEN AND FIRM MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 1880 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 282 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90816 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/15/99 STUDY DAY OF DEATH: 598 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 565.0 GRAMS DATE AND TIME OF NECROPSY: 09/15/99 14:14 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1881 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 283 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90817 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 341.5 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:00 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 9.6870 2.8366 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1882 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 284 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90817 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 341.5 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:00 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 1883 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 285 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90818 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 515.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:27 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0377 .0073 % .0176 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0320 .0062 % .0150 WEIGHT TAKEN BRAIN (BR) 2.1395 .4150 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.1015 .4077 % .9822 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.0749 .4025 % .9698 WEIGHT TAKEN LIVER (LI) 16.4851 3.1979 % 7.7051 WEIGHT TAKEN LUNG (LU) 1.7990 .3490 % .8409 WEIGHT TAKEN SPLEEN (SP) .8540 .1657 % .3992 WEIGHT TAKEN LEFT TESTIS (TE0) 1.7136 .3324 % .8009 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.8148 .3520 % .8482 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0145 .0028 % .0068 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0084 .0016 % .0039 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN 1884 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 286 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90818 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 515.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:27 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 1885 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 287 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90819 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/28/99 14:10 PROSECTOR: JARED FISHEL RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; EYE (EY) : EYE (EY) : VERIFIED AT NECROPSY: OBSERVED -COLLAPSED GLOBE-ANTE MORTEM; LEFT: >UNREMARKABLE RED BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, DIFFUSE PANCREAS (PA) : -INFLAMMATION, VASCULAR,-SLIGHT, MULTI- FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MODERATELY SEVERE, FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT TESTIS (TE) : -MINERALIZATION,-MINIMAL, MULTI-FOCAL LUNG (LU) : -FIBROSIS, PLEURAL/SUBPLEURAL,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL 1886 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 288 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90819 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/28/99 14:10 PROSECTOR: JARED FISHEL RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 5 MM IN DIAMETER, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL GREY, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -CYST, EPIDERMAL INCLUSION,-PRESENT EPIDIDYMIDES (EP) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -------------------------------------------- 1887 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 289 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90819 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/28/99 14:10 PROSECTOR: JARED FISHEL RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), SPLEEN (SP), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1888 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 290 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90820 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/25/99 STUDY DAY OF DEATH: 485 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 648.3 GRAMS DATE AND TIME OF NECROPSY: 05/25/99 12:49 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NECROPSY: OBSERVED FOCAL >RESPIRATION; LABORED; VERIFIED AT LUNG (LU) : NECROPSY: NOT APPLICABLE -MINERALIZATION, VASCULAR,-MINIMAL, >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED FOCAL AT NECROPSY: NOT APPLICABLE PITUITARY (PI) : PITUITARY (PI) : >APPEARANCE; HUNCHED; VERIFIED AT -MASS(ES); 12 X 10 X 9 MM, RED, DARK -HEMORRHAGE,-MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE RED, GREY, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -------------------------------------------- 1889 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 291 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90820 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/25/99 STUDY DAY OF DEATH: 485 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 648.3 GRAMS DATE AND TIME OF NECROPSY: 05/25/99 12:49 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1890 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 292 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90821 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 695 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/21/99 9:45 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; OPAQUE; EYE-LEFT; VERIFIED AT EYE (EY) : EYE (EY) : NECROPSY: OBSERVED -OPAQUE CORNEA; LEFT >UNREMARKABLE >SKIN & PELAGE; SORE/SCAB; PAW-HIND- KIDNEY (KD) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL, FOCAL -VACUOLATION,-MINIMAL, FOCAL THYMUS (TH) : -CYST,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI- FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT POSTERIOR -B-ADENOMA,-NEOPLASM PRESENT ASPECT: SINGLE DARK RED AREA, 2 MM IN DIAMETER 1891 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 293 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90821 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 695 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/21/99 9:45 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HIND FOOT: -EROSION,-MODERATE, MULTI-FOCAL SINGLE DARK RED AND DRY AREA, 12 X 10 -FIBROSIS,-MODERATE, MULTI-FOCAL X 7 MM; COLLECTED -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- 1892 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 294 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90821 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 695 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/21/99 9:45 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1893 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 295 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90822 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 516.1 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:10 PROSECTOR: TODD ACKER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; NO FECES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >MASS(ES); DORSAL; WEEK 76; 10 X 10MM; -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- VERIFIED AT NECROPSY: OBSERVED FOCAL >SKIN & PELAGE; SORE/SCAB; TAIL- -VACUOLATION, HEPATOCELLULAR,-MODERATE, PROXIMAL; VERIFIED AT NECROPSY: MULTI-FOCAL OBSERVED KIDNEY (KD) : -INFARCT,-SLIGHT, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL -PYELONEPHRITIS,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 8 X 8 X 5 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, DARK RED, GRAY, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI- FOCAL 1894 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 296 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90822 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 516.1 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:10 PROSECTOR: TODD ACKER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : SKIN (SK) : -MASS(ES); MID DORSAL LUMBAR REGION: -B-KERATOACANTHOMA,-NEOPLASM PRESENT SINGLE MASS, 17 X 15 X 9 MM, TAN AND SOFT; CUT SURFACE EXUDES A TAN, CASEOUS SEMISOLID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT TAIL (TI) : TAIL (TI) : -ABRASION/ULCERATIONS; NEAR BASE OF -EROSION,-MODERATE, MULTI-FOCAL TAIL: SINGLE BROWN AND MOIST ABRADED -INFLAMMATION, ACUTE,-MODERATELY AREA, 7 X 5 MM; COLLECTED SEVERE, MULTI-FOCAL -B-KERATOACANTHOMA,-NEOPLASM PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 1895 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 297 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90822 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 516.1 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:10 PROSECTOR: TODD ACKER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1896 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 298 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90823 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 760.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:40 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -DEGENERATION, CYSTIC,-MODERATE, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL URINARY BLADDER (UB) : -CALCULUS,-PRESENT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; SURROUNDING URETHRA: GREEN -INFLAMMATION, ACUTE,-SLIGHT, MULTI- AND FIRM FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -BONE MARROW SMEAR TAKEN -------------------------------------------- 1897 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 299 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90823 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 760.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:40 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), LUNG (LU), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 1898 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 300 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90824 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 679.7 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 15:06 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NO FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; YELLOW HAIR COAT; -DILATATION, VENTRICULAR,-PRESENT VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : -MASS(ES); PAPILLARY PROCESS: SINGLE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- MASS, 13 X 18 X 26 MM, FIRM, FOCAL LOBULATED, RED, DARK RED, TAN AND -VACUOLATION, HEPATOCELLULAR,-SEVERE, YELLOW; SAME ON CUT SURFACE; FOCAL COLLECTED; REGIONAL LN (HEPATIC) NOT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM IDENTIFIED PRESENT KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -CAST, PROTEINACEOUS,-SLIGHT, MULTI- -CALCULUS(I); BILATERAL, WITHIN FOCAL PELVIS: MULTIPLE HARD, TAN AND WHITE -DILATATION, PELVIC,-PRESENT CALCULI, UP TO 2 X 2 X 4 MM -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT, MULTI- FOCAL THYROID (TY) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 1899 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 301 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90824 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 679.7 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 15:06 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 5 X 6 X 9 MM, -HEMORRHAGE,-SEVERE, MULTI-FOCAL SOFT, LOBULATED, RED, DARK RED AND TAN -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL CAUSE OF DEATH (CD) : -HEPATOCELLULAR ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL -BLOOD FILM TAKEN -ANIMAL OBESE -------------------------------------------- 1900 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 302 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90824 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 679.7 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 15:06 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1901 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 303 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90825 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/11/99 STUDY DAY OF DEATH: 685 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/11/99 10:10 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: >UNREMARKABLE MULTIPLE DARK RED AREAS, UP TO 10 X 5 MM; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATELY SEVERE, DIFFUSE PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -CHRONIC PROGRESSIVE NEPHROPATHY,- PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- 1902 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 304 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90825 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/11/99 STUDY DAY OF DEATH: 685 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/11/99 10:10 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1903 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 305 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90826 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/29/99 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/29/99 13:44 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR,-SLIGHT, FOCAL KIDNEY (KD) : -INFARCT,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI- FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; RIGHT: 8 X 6 X 5 MM (LEFT IS 3 -HYPERPLASIA, LYMPHOCYTIC,-MINIMAL, X 2 X 1 MM) (CAP 22) MULTI-FOCAL 1904 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 306 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90826 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/29/99 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/29/99 13:44 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, LUMBAR/ILIAC (LM) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 22) GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- 1905 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 307 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90826 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/29/99 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/29/99 13:44 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1906 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 308 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90827 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/11/99 STUDY DAY OF DEATH: 471 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 924.3 GRAMS DATE AND TIME OF NECROPSY: 05/11/99 15:02 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >RESPIRATION; LABORED; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED -DILATATION, VENTRICULAR,-PRESENT AT NECROPSY: NOT APPLICABLE -HEMORRHAGE,-MODERATE, MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- >DISCHARGE; ORAL; CLEAR; VERIFIED AT FOCAL NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 11 X 7 X 5 MM, -HEMORRHAGE,-SEVERE, MULTI-FOCAL DARK RED, SOFT AND LOBULATED -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1907 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 309 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90827 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/11/99 STUDY DAY OF DEATH: 471 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 924.3 GRAMS DATE AND TIME OF NECROPSY: 05/11/99 15:02 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); BILATERAL, PECTORAL REGIONS: -GALACTOCELE,-MODERATELY SEVERE, MULTI- MULTIPLE TAN AND CREAMY SEMIFLUID FOCAL FILLED CYSTS, UP TO 3 MM IN DIAMETER -MINERALIZATION,-MINIMAL, MULTI-FOCAL (CAP 21) GENERAL COMMENT (GC) : -DISCHARGE-ORAL; CLEAR AND MOIST -BLOOD FILM TAKEN -ANIMAL OBESE -------------------------------------------- 1908 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 310 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90827 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/11/99 STUDY DAY OF DEATH: 471 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 924.3 GRAMS DATE AND TIME OF NECROPSY: 05/11/99 15:02 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1909 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 311 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90828 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/08/99 STUDY DAY OF DEATH: 560 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/08/99 8:38 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; DILATED PUPIL; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 7 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT -------------------------------------------- 1910 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 312 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90828 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/08/99 STUDY DAY OF DEATH: 560 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/08/99 8:38 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1911 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 313 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90829 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1188.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:39 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK LIVER (LI) : LIVER (LI) : 91; 20 X 15MM; VERIFIED AT NECROPSY: -RED FOCUS(I)/AREA(S); ALL LOBES: -FOCUS, ALTERED HEPATOCELLULAR, NOT OBSERVED MULTIPLE DARK RED AREAS, UP TO 3 MM IN EOSINOPHILIC,-MODERATE, FOCAL >SKIN & PELAGE; SORE/SCAB; AXILLARY DIAMETER; COLLECTED ON ROUTINE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- REGION-RIGHT; VERIFIED AT NECROPSY: SECTIONS FOCAL OBSERVED -TELANGIECTASIS,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MODERATE, FOCAL PANCREAS (PA) : PANCREAS (PA) : -MASS(ES); NEAR SPLEEN: SINGLE MASS, 6 -ATROPHY, ACINAR,-MODERATE, MULTI-FOCAL MM IN DIAMETER, RED, TAN AND FIRM; -B-ADENOMA, ISLET CELL,-NEOPLASM SAME ON CUT SURFACE (CAP 23); REGIONAL PRESENT LN (SEE PANCREATIC) THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT SKIN (SK) : SKIN (SK) : -ABRASION(S)/ULCERATION(S); RIGHT -INFLAMMATION, ACUTE,-SLIGHT, FOCAL AXILLARY REGION: SINGLE TAN, RED -ULCERATION,-MODERATELY SEVERE, FOCAL ABRADED AREA, 17 MM IN DIAMETER (CAP -B-FIBROMA,-NEOPLASM PRESENT 22) 1912 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 314 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90829 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1188.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:39 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -CYST(S); SINGLE CLEAR FLUID FILLED -DILATATION, SINUSOIDAL,-MODERATE, CYST, 4 MM IN DIAMETER (CAP 27) MULTI-FOCAL LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -DIFFUSELY RED; (CAP 24) -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -CYSTS; BILATERAL: MULTIPLE CLEAR -DILATATION, SINUSOIDAL,-MODERATE, FLUID FILLED CYSTS ON EACH, UP TO 7 MM FOCAL IN DIAMETER (CAP 26) BILE DUCT (BD) : BILE DUCT (BD) : -LARGE; UP TO 2 MM IN DIAMETER (CAP -DILATED,-PRESENT 25) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 1913 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 315 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90829 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1188.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:39 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 1914 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 316 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90830 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 783.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:00 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -FOCUS, ALTERED HEPATOCELLULAR, MULTIPLE TAN AREAS, UP TO 5 X 4 MM; EOSINOPHILIC,-MINIMAL, FOCAL COLLECTED ON ROUTINE SECTIONS -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-SEVERE, FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; -HEMORRHAGE,-SLIGHT, MULTI-FOCAL COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1915 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 317 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90830 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 783.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:00 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), LUNG (LU), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 1916 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 318 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90831 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 573.2 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:27 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0229 .0040 % .0100 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0237 .0041 % .0103 WEIGHT TAKEN BRAIN (BR) 2.2907 .3996 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.0209 .3526 % .8822 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.0483 .3573 % .8942 WEIGHT TAKEN LIVER (LI) 18.1672 3.1694 % 7.9309 WEIGHT TAKEN LUNG (LU) 1.6127 .2814 % .7040 WEIGHT TAKEN SPLEEN (SP) .8274 .1443 % .3612 WEIGHT TAKEN LEFT TESTIS (TE0) 1.8061 .3151 % .7884 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.8342 .3200 % .8007 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0125 .0022 % .0055 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0119 .0021 % .0052 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; ADRENAL, CORTEX (AC) : VERIFIED AT NECROPSY: OBSERVED -VACUOLATION,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 21) >NOT REQUIRED TO BE EXAMINED FOR ANIMAL GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 1917 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 319 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90831 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 573.2 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:27 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 1918 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 320 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90832 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 854.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:34 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >APPEARANCE; MISSING; TEETH; VERIFIED EOSINOPHILIC,-SLIGHT, MULTI-FOCAL AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 8 MM IN DIAMETER (RIGHT -ANGIECTASIS,-MODERATE, MULTI-FOCAL IS 5 MM IN DIAMETER) -HEMORRHAGE,-MODERATE, MULTI-FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT 1919 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 321 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90832 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 854.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:34 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ URINARY BLADDER (UB) : -CALCULUS,-PRESENT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -MINERALIZATION, LUMINAL,-MODERATELY URETHRA: TAN AND FIRM SEVERE, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL: UP TO 11 X 8 X 4 MM -DILATATION, SINUSOIDAL,-SLIGHT, FOCAL (CAP 22) -HEMORRHAGE,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR: BROKEN OFF AT BASE -ANIMAL OBESE -------------------------------------------- 1920 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 322 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90832 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 854.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:34 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 1921 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 323 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90833 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 364.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:50 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 11.3215 3.1077 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1922 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 324 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90833 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 364.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:50 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 1923 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 325 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90834 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 376.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:18 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 11.5017 3.0533 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1924 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 326 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90834 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 376.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:18 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 1925 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 327 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90835 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 634.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:55 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: OBSERVED -LARGE PELVIS(ES); BILATERAL -CYST,-SLIGHT, MULTI-FOCAL -DIFFUSELY LIGHT; BILATERAL: TAN -DILATATION, PELVIC,-PRESENT -CYST(S); BILATERAL, DIFFUSE: MULTIPLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- CLEAR FLUID FILLED CYSTS, UP TO 1 MM SEVERE, DIFFUSE IN DIAMETER -LARGE; BILATERAL: EACH IS 31 X 23 X 15 MM PANCREAS (PA) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT TESTIS (TE) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS -BONE MARROW SMEAR TAKEN -------------------------------------------- 1926 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 328 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90835 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 634.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:55 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), LUNG (LU), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 1927 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 329 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90836 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 513.3 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:13 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0284 .0055 % .0138 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0274 .0053 % .0133 WEIGHT TAKEN BRAIN (BR) 2.0529 .3999 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.8193 .3544 % .8862 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.9550 .3809 % .9523 WEIGHT TAKEN LIVER (LI) 14.7082 2.8654 % 7.1646 WEIGHT TAKEN LUNG (LU) 1.5994 .3116 % .7791 WEIGHT TAKEN SPLEEN (SP) .7967 .1552 % .3881 WEIGHT TAKEN LEFT TESTIS (TE0) 1.6068 .3130 % .7827 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.5707 .3060 % .7651 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0101 .0020 % .0049 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0099 .0019 % .0048 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MINIMAL, DIFFUSE KIDNEY (KD) : -DEGENERATION, TUBULAR,-SLIGHT, MULTI- FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 21) >NOT REQUIRED TO BE EXAMINED FOR ANIMAL GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 1928 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 330 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90836 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 513.3 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:13 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 1929 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 331 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90837 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 396.6 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 13:09 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); HEAD-MAXILLARY-RIGHT; WEEK LIVER (LI) : LIVER (LI) : 61; 20 X 25MM; VERIFIED AT NECROPSY: -LARGE; ALL LOBES: MARGINS APPEAR -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- OBSERVED ROUNDED FOCAL >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED MINIMAL, MULTI-FOCAL AT NECROPSY: NOT APPLICABLE PITUITARY (PI) : >SKIN & PELAGE; SORE/SCAB; HEAD- -HYPERPLASIA,-MINIMAL, MULTI-FOCAL MAXILLARY-RIGHT; VERIFIED AT NECROPSY: HARDERIAN GLAND (HG) : OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >EYES; RED DISCHARGE; EYE-RIGHT; FOCAL VERIFIED AT NECROPSY: OBSERVED COLON (CO) : -PARASITISM,-PRESENT SKIN (SK) : SKIN (SK) : -MASS(ES); RIGHT SIDE OF HEAD: SINGLE -EDEMA,-MODERATE, FOCAL MASS, 34 X 26 X 24 MM, TAN, RED, -INFLAMMATION, ACUTE,-MODERATELY VASCULAR AND LOBULATED; ON SECTION, SEVERE, MULTI-FOCAL EXUDES A TAN GRANULAR SEMISOLID; COLLECTED INTACT; OVERLYING SKIN HAS MULTIPLE RED, MOIST ULCERATED AREAS, UP TO 7 MM IN DIAMETER; COLLECTED INTACT WITH MASS; REGIONAL LN (SEE MANDIBULAR) 1930 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 332 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90837 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 396.6 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 13:09 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ URINARY BLADDER (UB) : URINARY BLADDER (UB) : -THICKENED WALL; DIFFUSE -HYPERPLASIA, TRANSITIONAL CELL,- SLIGHT, DIFFUSE -INFLAMMATION, CHRONIC,-MODERATE, MULTI- FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC,-MODERATE, MULTI- FOCAL SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL CAUSE OF DEATH (CD) : -AUDITORY SEBACEOUS GLAND CARCINOMA,- PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; RIGHT: 7 X 4 X 2 MM (FOR -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, COMPARISON, LEFT IS 4 X 2 X 1 MM) (CAP DIFFUSE 21) MAMMARY, MALE (MM) : -MINERALIZATION,-MODERATE, FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT AUDITORY SEB GL (AS) : -M-CARCINOMA,-NEOPLASM PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DRY RED MATERIAL -ANIMAL THIN 1931 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 333 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90837 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 396.6 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 13:09 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -GI TRACT-LARGE LUMEN; ENTIRE GI TRACT DISTENDED WITH GAS -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1932 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 334 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90838 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 900.0 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:11 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); MOUTH; WEEK 91; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : LIVER (LI) : -LARGE; ALL LOBES >UNREMARKABLE KIDNEY (KD) : KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -CYST,-MODERATELY SEVERE, MULTI-FOCAL -DIFFUSELY LIGHT; BILATERAL: TAN -NEPHROPATHY, CHRONIC PROGRESSIVE,- -CYST(S); LEFT, CORTEX: SINGLE CLEAR SEVERE, DIFFUSE FLUID FILLED CYST, 3 MM IN DIAMETER -LARGE; BILATERAL: EACH IS 26 X 20 X 14 MM PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL -INFLAMMATION, VASCULAR,-MODERATE, FOCAL -PIGMENT,-MINIMAL, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL HEART (HT) : HEART (HT) : -LIGHT FOCUS(I)/AREA(S); EPICARDIUM OF -DEGENERATION/NECROSIS,-SLIGHT, MULTI- BOTH VENTRICLES: MULTIPLE GRAY FOCAL INDISTINCT AREAS -FIBROSIS,-SLIGHT, MULTI-FOCAL 1933 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 335 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90838 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 900.0 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:11 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -HEMORRHAGE,-SLIGHT, FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 5 X 5 X 4 MM; APPEARS SWOLLEN -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : SKIN (SK) : -RAISED AREA(S); LEFT UPPER LIP: >UNREMARKABLE SINGLE RED, TAN, RAISED AREA, 4 X 3 X 2 MM (CAP 22) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 1934 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 336 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90838 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 900.0 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:11 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 1935 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 337 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90839 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/19/99 STUDY DAY OF DEATH: 693 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/19/99 15:03 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; LIMB-HIND-LEFT; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: NOT OBSERVED -LARGE; ALL LOBES >UNREMARKABLE >SKIN & PELAGE; BROWN HAIR COAT; KIDNEY (KD) : KIDNEY (KD) : VENTRAL-ABDOMINAL; VERIFIED AT -LARGE; RIGHT: 31 X 21 X 15 MM (LEFT, -HEMORRHAGE,-MODERATE, MULTI-FOCAL NECROPSY: OBSERVED SEE MASS COMMENT) -NECROSIS,-MODERATELY SEVERE, MULTI- >SKIN & PELAGE; SORE/SCAB; VENTRAL- -MASS(ES); INVOLVING ENTIRE LEFT: FOCAL CERVICAL; VERIFIED AT NECROPSY: SINGLE MASS, 55 X 50 X 45 MM, RED, -NEPHROPATHY, CHRONIC PROGRESSIVE,- OBSERVED DARK RED, GREY, TAN, VASCULAR, MODERATELY SEVERE, DIFFUSE LOBULATED AND FIRM; SAME ON CUT -M-NEPHROBLASTOMA,-NEOPLASM PRESENT SURFACE; COLLECTED INTACT; REGIONAL LN (RENAL) NORMAL (CAP 23) ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, MULTI-FOCAL TESTIS (TE) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL LUNG (LU) : LUNG (LU) : -UNCOLLAPSED; ALL LOBES -HEMORRHAGE,-SLIGHT, FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL 1936 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 338 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90839 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/19/99 STUDY DAY OF DEATH: 693 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/19/99 15:03 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PARATHYROID (PT) : -HYPERPLASIA,-MODERATELY SEVERE, DIFFUSE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); MID VENTRAL THORACIC -INFLAMMATION, ACUTE,-MINIMAL, FOCAL REGION: SINGLE DRY, DARK RED CRUSTED -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM AREA, 5 X 4 MM (CAP 22); POSSIBLY PRESENT CONSISTENT WITH SCAB (CERVICAL REGION) AS NOTED IN CLINICAL OBSERVATIONS PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -NEPHROBLASTOMA,-PRESENT LN, RENAL (RL) : -DILATATION, SINUSOIDAL,-MINIMAL, MULTI- FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS, EXTENDING TO VENTRAL ABDOMINAL REGION: DRY, BROWN MATERIAL -------------------------------------------- 1937 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 339 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90839 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/19/99 STUDY DAY OF DEATH: 693 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/19/99 15:03 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1938 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 340 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90840 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 810.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:44 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -VACUOLATION, HEPATOCELLULAR,-MINIMAL, >SKIN & PELAGE; BROWN HAIR COAT; MULTIPLE DARK RED AREAS, 3 MM IN MULTI-FOCAL PERINEAL AREA; VERIFIED AT NECROPSY: DIAMETER; COLLECTED ON ROUTINE SECTION OBSERVED KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -LARGE; UP TO 8 MM IN DIAMETER -HEMORRHAGE,-MODERATELY SEVERE, MULTI- -DIFFUSELY RED; DARK RED FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT, MULTI-FOCAL -M-HEMANGIOSARCOMA,-NEOPLASM PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATELY SEVERE, MULTI-FOCAL -EROSION,-MODERATELY SEVERE, MULTI- FOCAL 1939 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 341 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90840 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 810.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:44 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); BILATERAL, HINDFEET: -EXOSTOSES,-MODERATELY SEVERE, MULTI- SINGLE DARK RED AND MOIST CRUSTED AREA FOCAL ON EACH, RIGHT IS 15 MM IN DIAMETER, -INFLAMMATION, ACUTE,-MODERATE, MULTI- LEFT IS 17 X 12 MM; BOTH COLLECTED FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; INVOLVING ENTIRE PERIANAL REGION: BROWN AND MOIST -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1940 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 342 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90840 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 810.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:44 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 1941 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 343 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90841 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/03/99 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 504.1 GRAMS DATE AND TIME OF NECROPSY: 12/03/99 14:21 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- EYE (EY) : EYE (EY) : LEFT; VERIFIED AT NECROPSY: OBSERVED -OPAQUE CORNEA; LEFT -FIBROSIS, CORNEAL,-MODERATE, MULTI- >SKIN & PELAGE; YELLOW HAIR COAT; -COLLAPSED GLOBE-ANTE MORTEM; LEFT FOCAL VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, ACUTE, CORNEAL,- >EXCRETION; FEW FECES; VERIFIED AT MODERATE, MULTI-FOCAL NECROPSY: NOT APPLICABLE -VASCULARIZATION, CORNEAL,-MODERATELY >APPEARANCE; THIN; VERIFIED AT SEVERE, MULTI-FOCAL NECROPSY: NOT OBSERVED LIVER (LI) : >EYES; DISCOLORED/LIGHT RED; EYES; -HYPERPLASIA, BILE DUCT,-SLIGHT, MULTI- VERIFIED AT NECROPSY: NOT APPLICABLE FOCAL >APPEARANCE; HUNCHED; VERIFIED AT -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >RESPIRATION; LABORED; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL -PYELONEPHRITIS,-SLIGHT, MULTI-FOCAL PANCREAS (PA) : -INFLAMMATION, VASCULAR,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -SMALL; LEFT: 3 X 3 X 2 MM (RIGHT IS 5 -DEGENERATION, CYSTIC, CORTICAL,- X 4 X 3 MM) MINIMAL, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL 1942 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 344 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90841 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/03/99 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 504.1 GRAMS DATE AND TIME OF NECROPSY: 12/03/99 14:21 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: BOTH ARE 17 X 10 X -ATROPHY/DEGENERATION,-SLIGHT, DIFFUSE 5 MM; BOTH ARE SOFT THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, MULTI-FOCAL PARATHYROID (PT) : -HYPERPLASIA,-MODERATE, DIFFUSE PITUITARY (PI) : PITUITARY (PI) : -DIFFUSELY RED -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL -MINERALIZATION, LUMINAL,-MINIMAL, FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: LEFT IS 15 X 10 X >UNREMARKABLE 10 MM, RIGHT IS 15 X 6 X 2 MM -MOTTLED; LEFT: RED, TAN AND BROWN CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT 1943 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 345 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90841 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/03/99 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 504.1 GRAMS DATE AND TIME OF NECROPSY: 12/03/99 14:21 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -DIFFUSELY RED; DARK RED (CAP 22) -DILATATION, SINUSOIDAL,-SLIGHT, MULTI- FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: -EDEMA,-MODERATELY SEVERE, MULTI-FOCAL SINGLE TAN AREA, 4 MM IN DIAMETER; -EROSION,-MODERATE, FOCAL COLLECTED -FIBROSIS,-MODERATELY SEVERE, FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL AORTA (AO) : -MINERALIZATION,-SLIGHT, MULTI-FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; EXTENDING INTO VENTRAL ABDOMINAL REGION: YELLOW -BLOOD FILM TAKEN -FLUID-THORACIC CAVITY; CLEAR FLUID -------------------------------------------- 1944 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 346 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90841 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/03/99 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 504.1 GRAMS DATE AND TIME OF NECROPSY: 12/03/99 14:21 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), SPLEEN (SP), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1945 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 347 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90842 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 559.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:42 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC -DEGENERATION, CYSTIC,-MODERATELY SURFACE OF RIGHT MEDIAN LOBE: SINGLE SEVERE, FOCAL RED AREA, 2 MM IN DIAMETER; COLLECTED -FOCUS, ALTERED HEPATOCELLULAR, ON ROUTINE SECTION EOSINOPHILIC,-MODERATE, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -MOTTLED; DARK RED AND TAN -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL TESTIS (TE) : TESTIS (TE) : -SMALL; LEFT: 18 X 8 X 7 MM (RIGHT IS -ATROPHY/DEGENERATION,-MODERATELY 25 X 15 X 8 MM) SEVERE, MULTI-FOCAL -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1946 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 348 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90842 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 559.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:42 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN (CAP -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 1947 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 349 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90843 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 523.7 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:09 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0292 .0056 % .0128 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0292 .0056 % .0128 WEIGHT TAKEN BRAIN (BR) 2.2810 .4356 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.8280 .3491 % .8014 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.8980 .3624 % .8321 WEIGHT TAKEN LIVER (LI) 17.8078 3.4004 % 7.8070 WEIGHT TAKEN LUNG (LU) 1.7571 .3355 % .7703 WEIGHT TAKEN SPLEEN (SP) .7643 .1459 % .3351 WEIGHT TAKEN LEFT TESTIS (TE0) 1.5660 .2990 % .6865 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.5425 .2945 % .6762 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0126 .0024 % .0055 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0108 .0021 % .0047 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MINIMAL, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 1948 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 350 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90843 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 523.7 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:09 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 1949 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 351 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90844 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 669.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:24 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; PAW-HIND-RIGHT; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -MOTTLED; ALL LOBES: RED AND TAN -DEGENERATION, CYSTIC,-SLIGHT, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, FOCAL -CYST, ULTIMOBRANCHIAL,-PRESENT -HYPERPLASIA, C-CELL,-SLIGHT, FOCAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 4 X 3 MM -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE -EDEMA,-MODERATELY SEVERE, MULTI-FOCAL TAN AND BROWN ERODED AREA, 8 X 2 MM -EROSION,-MODERATE, MULTI-FOCAL -THICKENED WALL; DIFFUSE URINARY BLADDER (UB) : -CALCULUS,-PRESENT 1950 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 352 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90844 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 669.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:24 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC,-MODERATE, MULTI- URETHRA: GREEN AND FIRM FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; RIGHT TARSAL JOINT: 17 MM IN -EDEMA,-MODERATELY SEVERE, MULTI-FOCAL DIAMETER (LEFT IS 10 MM IN DIAMETER); -EXOSTOSES,-MODERATE, MULTI-FOCAL COLLECTED -INFLAMMATION, ACUTE,-MINIMAL, MULTI- FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1951 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 353 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90844 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 669.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:24 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU) ------------------------------------------------------------------------------------------------------------------------------------ 1952 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 354 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90845 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/26/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 645.2 GRAMS DATE AND TIME OF NECROPSY: 07/26/99 13:35 PROSECTOR: LYNN HORNER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; BROWN HAIR COAT; -DILATATION, VENTRICULAR,-PRESENT GENERALIZED; VERIFIED AT NECROPSY: NOT LIVER (LI) : LIVER (LI) : OBSERVED -RED FOCUS(I)/AREA(S); LEFT LATERAL -VACUOLATION, HEPATOCELLULAR,- >EXCRETION; NO FECES; VERIFIED AT LOBE: MULTIPLE DARK RED AREAS, UP TO 4 MODERATELY SEVERE, MULTI-FOCAL NECROPSY: NOT APPLICABLE X 3 MM; COLLECTED ON ROUTINE SECTION >SKIN & PELAGE; BROWN SKIN; PAWS-ALL; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-SLIGHT, MULTI- FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 8 X 7 X 6 MM, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL DARK RED, GREY AND SOFT -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,- URETHRA: GREEN AND FIRM MODERATE, MULTI-FOCAL 1953 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 355 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90845 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/26/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 645.2 GRAMS DATE AND TIME OF NECROPSY: 07/26/99 13:35 PROSECTOR: LYNN HORNER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; PERIANAL REGION: MOIST BROWN MATERIAL -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ALL FOUR FEET: DARK BROWN, MOIST MATERIAL -------------------------------------------- 1954 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 356 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90845 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/26/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 645.2 GRAMS DATE AND TIME OF NECROPSY: 07/26/99 13:35 PROSECTOR: LYNN HORNER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1955 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 357 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90846 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 324.2 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:45 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 9.5115 2.9338 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1956 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 358 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90846 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 324.2 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:45 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 1957 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 359 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90847 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 687 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 389.8 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 14:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; NO FECES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -LIGHT FOCUS(I)/AREA(S); RIGHT MEDIAN -LIPIDOSIS, SUBCAPSULAR (TENSION NECROPSY: OBSERVED LOBE, ALONG MARGIN NEAR HILUS: SINGLE LIPIDOSIS),-SEVERE, FOCAL >SKIN & PELAGE; YELLOW HAIR COAT; TAN AREA, 7 X 3 MM; COLLECTED ON -VACUOLATION, HEPATOCELLULAR,-MODERATE, VERIFIED AT NECROPSY: NOT OBSERVED ROUTINE SECTION MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -DARK FOCUS(I)/AREA(S); DIAPHRAGMATIC NECROPSY: NOT APPLICABLE SURFACE OF LEFT LATERAL LOBE: SINGLE BROWN FOCUS, 1 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SEVERE, DIFFUSE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -SMALL; LEFT: 4 MM IN DIAMETER (RIGHT -VACUOLATION,-MINIMAL, MULTI-FOCAL IS 6 MM IN DIAMETER) LN, MESENTERIC (MS) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION,-MINIMAL, FOCAL 1958 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 360 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90847 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 687 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 389.8 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 14:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 9 X 9 MM, RED, GREY, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,- URETHRA: GREEN AND FIRM MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -ANIMAL THIN -------------------------------------------- 1959 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 361 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90847 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 687 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 389.8 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 14:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1960 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 362 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90848 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/05/99 STUDY DAY OF DEATH: 526 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 973.1 GRAMS DATE AND TIME OF NECROPSY: 07/05/99 11:22 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -MOTTLED; ALL LOBES: RED AND TAN -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >DISCHARGE; NASAL; RED IN COLOR; FOCAL VERIFIED AT NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR,-MODERATE, >RESPIRATION; AUDIBLE; VERIFIED AT MULTI-FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -MINERALIZATION, PELVIC,-MODERATE, NECROPSY: OBSERVED MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, MULTI-FOCAL -VACUOLATION,-SLIGHT, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 1961 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 363 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90848 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/05/99 STUDY DAY OF DEATH: 526 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 973.1 GRAMS DATE AND TIME OF NECROPSY: 07/05/99 11:22 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; RIGHT: DRY RED MATERIAL -DISCHARGE-NASAL; DRY RED MATERIAL -MALOCCLUSION; BILATERAL: UPPER INCISORS GENERAL INFORMATION (XX) : >NOTE:>RED DISCHARGE (LEFT EYE) NOTED IN CLINICAL OBSERVATIONS WAS NOT SEEN AT NECROPSY -------------------------------------------- 1962 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 364 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90848 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/05/99 STUDY DAY OF DEATH: 526 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 973.1 GRAMS DATE AND TIME OF NECROPSY: 07/05/99 11:22 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1963 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 365 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90849 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/19/99 11:20 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED -DILATATION, VENTRICULAR,-PRESENT AT NECROPSY: NOT APPLICABLE LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -HYPERPLASIA, BILE DUCT,-SLIGHT, MULTI- NECROPSY: NOT OBSERVED FOCAL >EXCRETION; NONFORMED FECES; VERIFIED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- AT NECROPSY: NOT APPLICABLE FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT CECUM (CE) : -PARASITISM,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1964 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 366 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90849 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/19/99 11:20 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL, LUMBAR: LEFT IS 9 X -DILATATION, SINUSOIDAL,-MODERATE, 3 X 2 MM, RIGHT IS 13 X 7 X 5 MM (CAP MULTI-FOCAL 22) -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, MULTI-FOCAL MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); RIGHT -EROSION,-MODERATE, FOCAL HINDFOOT: SINGLE RED, TAN, MOIST, -FIBROSIS,-MODERATE, FOCAL SLIGHTLY RAISED ABRADED AREA, 13 X 10 -INFLAMMATION, ACUTE,-SLIGHT, FOCAL X 4 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 1965 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 367 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90849 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/19/99 11:20 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1966 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 368 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90850 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 579.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:02 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LIVER (LI) : LIVER (LI) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -DEGENERATION, CYSTIC,-MINIMAL, FOCAL >APPEARANCE; MALOCCLUSION; VERIFIED AT MULTIPLE RED AREAS, PINPOINT AND UP TO -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- NECROPSY: OBSERVED 1 MM IN DIAMETER; COLLECTED ON ROUTINE FOCAL SECTIONS KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); LEFT -CYST,-MODERATE, MULTI-FOCAL -ROUGH SURFACE, DIFFUSE; LEFT -DILATATION, PELVIC,-PRESENT -SMALL; LEFT: 19 X 10 X 8 MM (RIGHT IS -NEPHROPATHY, CHRONIC PROGRESSIVE,- 24 X 15 X 10 MM) MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 MM IN DIAMETER (RIGHT -DEGENERATION, CYSTIC, CORTICAL,- IS 4 X 3 X 2 MM) MINIMAL, FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL THYROID (TY) : -NECROSIS,-MODERATE, FOCAL -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 1967 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 369 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90850 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 579.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:02 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -LIGHT FOCUS(I)/AREA(S); MID ANTERIOR ASPECT: SINGLE TAN AREA, 2 MM IN DIAMETER STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -EDEMA,-MODERATE, MULTI-FOCAL TAN ERODED AREAS, UP TO 4 X 3 MM -EROSION,-MODERATE, MULTI-FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, URETHRA: GREEN AND FIRM MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: -EDEMA,-MODERATE, FOCAL SINGLE DARK RED AND TAN AREA, 4 MM IN -EROSION,-SLIGHT, FOCAL DIAMETER; COLLECTED -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: RED -MALOCCLUSION; BILATERAL, UPPER INCISORS -BONE MARROW SMEAR TAKEN -------------------------------------------- 1968 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 370 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90850 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 579.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:02 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 1969 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 371 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90851 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 792.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:11 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTIPLE TAN, SLIGHTLY RAISED AREAS, MULTI-FOCAL UP TO 3 X 2 MM; COLLECTED ON ROUTINE -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM SECTIONS PRESENT -DIFFUSELY LIGHT; ALL LOBES: TAN -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED FOCI, PINPOINT AND UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL -PIGMENT,-MINIMAL, FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); LEFT ASPECT: -CYST,-PRESENT SINGLE DARK BROWN AREA, 2 X 1 MM -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : URINARY BLADDER (UB) : -CALCULUS(I); WITHIN LUMEN: SINGLE TAN >UNREMARKABLE AND FIRM CALCULUS, 10 X 5 X 5 MM; COLLECTED INTACT 1970 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 372 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90851 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 792.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:11 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: EACH IS UP TO 10 X -DILATATION, SINUSOIDAL,-SLIGHT, MULTI- 6 X 6 MM (CAP 22) FOCAL -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL -EDEMA,-MODERATE, MULTI-FOCAL HINDFEET: SINGLE DARK BROWN ABRADED -EROSION,-MODERATE, MULTI-FOCAL AREA ON EACH, LEFT IS 9 X 7 X 5 MM, -FIBROSIS,-SLIGHT, MULTI-FOCAL RIGHT IS 5 MM IN DIAMETER; BOTH -INFLAMMATION, ACUTE,-MODERATE, MULTI- COLLECTED FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1971 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 373 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90851 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 792.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:11 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), TESTIS (TE), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 1972 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 374 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90852 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 508.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:17 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0262 .0052 % .0120 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0277 .0054 % .0126 WEIGHT TAKEN BRAIN (BR) 2.1921 .4311 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.9487 .3832 % .8890 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.9761 .3886 % .9015 WEIGHT TAKEN LIVER (LI) 15.8553 3.1181 % 7.2329 WEIGHT TAKEN LUNG (LU) 1.7581 .3457 % .8020 WEIGHT TAKEN SPLEEN (SP) .6186 .1217 % .2822 WEIGHT TAKEN LEFT TESTIS (TE0) 1.7403 .3422 % .7939 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.8014 .3543 % .8218 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0134 .0026 % .0061 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0151 .0030 % .0069 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 1973 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 375 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90852 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 508.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:17 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 1974 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 376 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90853 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 305.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:46 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 9.4927 3.1113 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1975 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 377 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90853 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 305.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:46 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 1976 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 378 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90854 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 746.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:02 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -DEGENERATION, CYSTIC,-MODERATELY SEVERE, MULTI-FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATELY SEVERE, MULTI- FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1977 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 379 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90854 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 746.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:02 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 1978 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 380 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90855 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 779.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:32 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); DORSAL-CERVICAL- LIVER (LI) : LEFT; WEEK 105; VERIFIED AT NECROPSY: -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- OBSERVED FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY RED; DARK RED >UNREMARKABLE LUNG (LU) : -FIBROSIS, PLEURAL/SUBPLEURAL,-SLIGHT, MULTI-FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); MID DORSAL CERVICAL -B-KERATOACANTHOMA,-NEOPLASM PRESENT REGION: SINGLE DRY BROWN CRUSTED AREA, 10 MM IN DIAMETER (CAP 22) URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL 1979 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 381 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90855 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 779.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:32 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 1980 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 382 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90856 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 897.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:39 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -DEGENERATION, CYSTIC,-MODERATE, MULTI- FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATELY SEVERE, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -MASS(ES); LEFT: SINGLE MASS, 19 X 15 >UNREMARKABLE X 11 MM, RED, DARK RED, TAN, SOFT AND LOBULATED; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE RENAL) ADRENAL, MEDULLA (MA) : -NECROSIS,-MODERATE, MULTI-FOCAL -M-MALIGNANT PHEOCHROMOCYTOMA,-NEOPLASM PRESENT 1981 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 383 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90856 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 897.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:39 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, RENAL (RL) : LN, RENAL (RL) : -DIFFUSELY RED; BILATERAL: DARK RED -HEMORRHAGE,-MODERATE, MULTI-FOCAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 1982 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 384 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90856 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 897.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:39 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 1983 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 385 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90857 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 452.5 GRAMS DATE AND TIME OF NECROPSY: 01/14/00 7:54 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; SORE/SCAB; PAW-HIND- -DILATATION, VENTRICULAR,-PRESENT LEFT; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >APPEARANCE; HUNCHED; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: NOT APPLICABLE -LARGE PELVIS(ES); BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- >BEHAVIOR; HYPOACTIVE; VERIFIED AT SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE TESTIS (TE) : -MINERALIZATION,-MINIMAL, FOCAL SPINAL CORD (SC) : -VACUOLATION, WHITE MATTER,-MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 14 X 10 X 5 MM, TAN, GRAY, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL AND SOFT -B-ADENOMA,-NEOPLASM PRESENT 1984 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 386 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90857 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 452.5 GRAMS DATE AND TIME OF NECROPSY: 01/14/00 7:54 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, DARK BROWN AREA, 2 MM IN DIAMETER MULTI-FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT HIND -EDEMA,-MODERATELY SEVERE, FOCAL FOOT: SINGLE DARK RED AND MOIST -EROSION,-MODERATELY SEVERE, FOCAL ABRADED AREA, 11 X 10 X 7 MM; -FIBROSIS,-MODERATE, FOCAL COLLECTED -INFLAMMATION, ACUTE,-MODERATE, FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 1985 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 387 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90857 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 452.5 GRAMS DATE AND TIME OF NECROPSY: 01/14/00 7:54 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1986 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 388 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90858 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 581.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:43 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -DEGENERATION, CYSTIC,-MODERATE, FOCAL MULTIPLE DARK RED AREAS, UP TO 2 MM IN -FOCUS, ALTERED HEPATOCELLULAR, DIAMETER; SOME ARE SUNKEN WITH A EOSINOPHILIC,-SLIGHT, FOCAL STELLATE PATTERN; COLLECTED ON ROUTINE -VACUOLATION, HEPATOCELLULAR,-MINIMAL, SECTIONS MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL PANCREAS (PA) : PANCREAS (PA) : -MASS(ES); ALONG SPLEEN: SINGLE MASS, -ATROPHY, ACINAR,-MODERATE, MULTI-FOCAL 3 MM IN DIAMETER, TAN AND FIRM; -M-CARCINOMA, ISLET CELL,-NEOPLASM COLLECTED INTACT WITH SPLEEN; REGIONAL PRESENT LN (SEE PANCREATIC) THYROID (TY) : THYROID (TY) : -LARGE; LEFT: 9 X 6 X 4 MM (RIGHT IS 6 -M-CARCINOMA, C-CELL,-NEOPLASM PRESENT X 2 X 1 MM) CECUM (CE) : CECUM (CE) : -THICKENED WALL; DIFFUSE >UNREMARKABLE URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1987 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 389 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90858 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 581.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:43 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -DIFFUSELY RED; DARK RED (CAP 22) -DILATATION, SINUSOIDAL,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -N-ISLET CELL CARCINOMA,-NEOPLASM PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 1988 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 390 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90858 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 581.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:43 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU) ------------------------------------------------------------------------------------------------------------------------------------ 1989 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 391 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90859 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/19/99 STUDY DAY OF DEATH: 479 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 538.6 GRAMS DATE AND TIME OF NECROPSY: 05/19/99 15:17 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; YELLOW HAIR COAT; -DILATATION, VENTRICULAR,-PRESENT VERIFIED AT NECROPSY: OBSERVED -HEMORRHAGE,-MODERATE, MULTI-FOCAL >RESPIRATION; AUDIBLE; VERIFIED AT -PIGMENT,-SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 15 X 10 X 3 -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE MM; BOTH ARE SOFT THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- BROWN, AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -EDEMA,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : >UNREMARKABLE 1990 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 392 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90859 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/19/99 STUDY DAY OF DEATH: 479 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 538.6 GRAMS DATE AND TIME OF NECROPSY: 05/19/99 15:17 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 5 X 2 MM EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST -BLOOD FILM TAKEN -------------------------------------------- 1991 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 393 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90859 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/19/99 STUDY DAY OF DEATH: 479 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 538.6 GRAMS DATE AND TIME OF NECROPSY: 05/19/99 15:17 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1992 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 394 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90860 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 570.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:40 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -MOTTLED; ALL LOBES: DARK RED AND -DEGENERATION, CYSTIC,-MODERATE, MULTI- BROWN FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-MINIMAL, FOCAL -TELANGIECTASIS,-SLIGHT, FOCAL KIDNEY (KD) : -CYST,-MINIMAL, FOCAL -INFARCT,-MODERATE, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); MID POSTERIOR -HEMORRHAGE,-SLIGHT, FOCAL ASPECT: SINGLE DARK RED AREA, 2 X 1 MM -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1993 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 395 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90860 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 570.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:40 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 1994 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 396 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90861 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/04/99 STUDY DAY OF DEATH: 495 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 456.3 GRAMS DATE AND TIME OF NECROPSY: 06/04/99 14:33 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NO FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; ROUGH HAIR COAT; -DILATATION, VENTRICULAR,-PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -CAST, PROTEINACEOUS,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED -MINERALIZATION, PELVIC,-SLIGHT, MULTI- AT NECROPSY: NOT APPLICABLE FOCAL >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED PITUITARY (PI) : PITUITARY (PI) : AT NECROPSY: NOT APPLICABLE -MASS(ES); 8 MM IN DIAMETER, RED, DARK -HEMORRHAGE,-SEVERE, DIFFUSE >SKIN & PELAGE; RED HAIR COAT; VENTRAL- RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT ABDOMINAL; VERIFIED AT NECROPSY: SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : OBSERVED -SMALL; BILATERAL: EACH IS 13 X 5 X 2 >UNREMARKABLE MM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED -DISCHARGE-NASAL; BILATERAL: RED -STAINS - SKIN/HAIRCOAT; VENTRAL MID ABDOMINAL REGION, HAIRCOAT: RED -------------------------------------------- 1995 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 397 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90861 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/04/99 STUDY DAY OF DEATH: 495 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 456.3 GRAMS DATE AND TIME OF NECROPSY: 06/04/99 14:33 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1996 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 398 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90862 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 435.0 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 15:30 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HUNCHED; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -DILATATION, VENTRICULAR,-PRESENT AT NECROPSY: NOT OBSERVED KIDNEY (KD) : KIDNEY (KD) : >BEHAVIOR; ATAXIC; VERIFIED AT -MOTTLED; BILATERAL: TAN AND BROWN -CAST, PROTEINACEOUS,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >RESPIRATION; IRREGULAR; VERIFIED AT LUNG (LU) : NECROPSY: NOT APPLICABLE -MINERALIZATION, VASCULAR,-MINIMAL, >APPEARANCE; THIN; VERIFIED AT FOCAL NECROPSY: NOT OBSERVED PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 5 X 8 X 12 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL SOFT, RED, DARK RED AND TAN -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -PIGMENT,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 1997 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 399 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90862 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 435.0 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 15:30 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1998 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 400 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90863 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 643.4 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:29 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0303 .0047 % .0123 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0309 .0048 % .0126 WEIGHT TAKEN BRAIN (BR) 2.4617 .3826 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.3739 .3690 % .9643 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.3341 .3628 % .9482 WEIGHT TAKEN LIVER (LI) 18.7061 2.9074 % 7.5989 WEIGHT TAKEN LUNG (LU) 2.3756 .3692 % .9650 WEIGHT TAKEN SPLEEN (SP) .7464 .1160 % .3032 WEIGHT TAKEN LEFT TESTIS (TE0) 1.7051 .2650 % .6927 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.7163 .2668 % .6972 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0100 .0016 % .0041 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0129 .0020 % .0052 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 1999 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 401 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90863 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 643.4 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:29 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2000 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 402 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90864 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 723.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:46 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 98; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT OBSERVED -MOTTLED; ALL LOBES: RED AND TAN -FOCUS, ALTERED HEPATOCELLULAR, CLEAR >SKIN & PELAGE; RED HAIR COAT; HEAD- CELL,-SLIGHT, FOCAL ENTIRE; VERIFIED AT NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL, MULTI-FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -RAISED AREA(S); LEFT ANTERIOR ASPECT: -CYST,-PRESENT SINGLE TAN AREA, 2 MM IN DIAMETER -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT 2001 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 403 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90864 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 723.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:46 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : -CRUSTED AREA(S); LEFT LATERAL SHOULDER REGION: SINGLE DARK RED AND TAN, SLIGHTLY RAISED AREA, 7 MM IN DIAMETER (CAP 22) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED, DARK RED, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL AND TAN; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD, HAIRCOAT: RED -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 2002 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 404 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90864 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 723.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:46 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2003 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 405 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90865 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 701.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 9:43 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- KIDNEY (KD) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATELY SEVERE, MULTI-FOCAL URINARY BLADDER (UB) : -CALCULUS,-PRESENT -DESQUAMATION, TRANSITIONAL EPITHELIUM,- MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HIND FOOT: -EDEMA,-MODERATE, FOCAL SINGLE MOIST RED CRUSTED AREA, 9 MM IN -EROSION,-MODERATELY SEVERE, FOCAL DIAMETER; COLLECTED -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2004 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 406 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90865 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 701.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 9:43 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2005 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 407 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90866 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 359.5 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:36 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 11.2529 3.1302 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2006 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 408 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90866 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 359.5 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:36 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2007 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 409 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90867 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 583.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:44 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 64; 20 X 20MM; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); VISCERAL -DEGENERATION, CYSTIC,-MODERATE, FOCAL >EYES; RED DISCHARGE; EYE-LEFT; SURFACE OF LEFT LATERAL LOBE: SINGLE -VACUOLATION, HEPATOCELLULAR,-MINIMAL, VERIFIED AT NECROPSY: OBSERVED RED AREA, 3 MM IN DIAMETER; COLLECTED MULTI-FOCAL ON ROUTINE SECTION KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL SKIN (SK) : SKIN (SK) : -MASS(ES); DORSAL LUMBAR REGION: -HEMORRHAGE,-MODERATE, FOCAL SINGLE MASS, 27 X 20 X 10 MM, TAN AND -INFLAMMATION, ACUTE,-MINIMAL, FOCAL FIRM; SAME ON CUT SURFACE; COLLECTED; -ULCERATION,-SLIGHT, FOCAL REGIONAL LN (INGUINAL) NORMAL (CAP -B-KERATOACANTHOMA,-NEOPLASM PRESENT 22); OVERLYING SKIN OF MASS HAS A SINGLE TAN AND BLACK ULCERATED AREA, 12 MM IN DIAMETER; COLLECTED INTACT WITH MASS URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2008 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 410 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90867 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 583.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:44 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: RED -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), THYROID (TY), LN, INGUINAL (IN) ------------------------------------------------------------------------------------------------------------------------------------ 2009 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 411 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90868 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 621.7 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 14:42 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED LIVER (LI) : AT NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR,-MODERATE, >EXCRETION; NO FECES; VERIFIED AT MULTI-FOCAL NECROPSY: NOT APPLICABLE ADRENAL, CORTEX (AC) : >RESPIRATION; IRREGULAR; VERIFIED AT -DEGENERATION, CYSTIC, CORTICAL,- NECROPSY: NOT APPLICABLE MODERATE, FOCAL >SKIN & PELAGE; RED HAIR COAT; VENTRAL- -HEMORRHAGE,-SLIGHT, FOCAL ABDOMINAL-LEFT; VERIFIED AT NECROPSY: HEART (HT) : HEART (HT) : OBSERVED >NOTE:>TRIMMING COMMENT: MISSING FROM >TISSUE MISSING >SKIN & PELAGE; YELLOW HAIR COAT; WET TISSUE VERIFIED AT NECROPSY: NOT OBSERVED PITUITARY (PI) : PITUITARY (PI) : >APPEARANCE; HUNCHED; VERIFIED AT -MASS(ES); SINGLE MASS, 14 X 8 X 7 MM, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- NECROPSY: NOT APPLICABLE RED, BROWN, TAN AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATE, MULTI-FOCAL -EROSION,-MODERATE, FOCAL -FIBROSIS,-SEVERE, MULTI-FOCAL 2010 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 412 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90868 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 621.7 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 14:42 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); BILATERAL, HINDFEET: -INFLAMMATION, CHRONIC ACTIVE,- SINGLE TAN, RED AND DRY AREA ON EACH, MODERATE, MULTI-FOCAL LEFT IS 5 X 3 MM, RIGHT IS 9 X 7 MM; BOTH COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; LEFT VENTRAL ABDOMINAL REGION: DARK RED AND DRY -------------------------------------------- 2011 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 413 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90868 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 621.7 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 14:42 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2012 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 414 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90869 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 877.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:42 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; SCROTUM; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, MULTI-FOCAL SKIN (SK) : SKIN (SK) : -ABRASION(S)/ULCERATION(S); PERINEAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL REGION: SINGLE TAN, RED AND MOIST -ULCERATION,-MODERATELY SEVERE, FOCAL ULCERATED AREA, 8 MM IN DIAMETER (CAP -B-FIBROMA,-NEOPLASM PRESENT 22) URINARY BLADDER (UB) : -EDEMA,-MODERATE, MULTI-FOCAL -HYPERPLASIA, TRANSITIONAL CELL,- MODERATE, DIFFUSE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL 2013 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 415 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90869 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 877.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:42 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -LARGE; UP TO 15 X 11 X 11 MM; APPEARS -DILATATION, SINUSOIDAL,-SEVERE, FOCAL CYSTIC (CAP 23) LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: UP TO 8 X 5 X 3 MM; -DILATATION, SINUSOIDAL,-MODERATELY APPEARS CYSTIC (CAP 24) SEVERE, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2014 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 416 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90869 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 877.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:42 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 2015 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 417 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90870 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 515.6 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 13:30 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; HEAD- BRAIN (BR) : BRAIN (BR) : ENTIRE; VERIFIED AT NECROPSY: NOT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; YELLOW HAIR COAT; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >APPEARANCE; THIN; VERIFIED AT FOCAL NECROPSY: OBSERVED TESTIS (TE) : TESTIS (TE) : >EXCRETION; FEW FECES; VERIFIED AT -CONTAINS FLUID; RIGHT: CLEAR FLUID -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE NECROPSY: NOT APPLICABLE >NOTE:>RIGHT TESTIS >BEHAVIOR; HYPOACTIVE; VERIFIED AT LUNG (LU) : NECROPSY: NOT APPLICABLE -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 8 X 12 MM, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- FIRM, RED, DARK RED, TAN, AND BROWN FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; DRY BROWN MATERIAL 2016 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 418 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90870 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 515.6 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 13:30 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DRY YELLOW MATERIAL -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2017 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 419 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90871 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 718.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:22 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); LEFT -CAST, PROTEINACEOUS,-MINIMAL, MULTI- -GRANULAR MATERIAL; LEFT, WITHIN FOCAL PELVIS: GREEN GRANULAR MATERIAL -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-MODERATE, FOCAL TESTIS (TE) : TESTIS (TE) : -SMALL; RIGHT: 15 X 10 X 8 MM (LEFT IS -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 23 X 14 X 10 MM) -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN 2018 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 420 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90871 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 718.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:22 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU) ------------------------------------------------------------------------------------------------------------------------------------ 2019 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 421 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90872 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/24/99 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/24/99 6:54 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT EYE (EY) : NECROPSY: NOT APPLICABLE -INFLAMMATION, ACUTE, CORNEAL,-MINIMAL, FOCAL -MINERALIZATION, CORNEAL,-SLIGHT, FOCAL -ULCERATION, CORNEAL,-MODERATE, FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT -SEMIFLUID MATERIAL-PELVIS; RIGHT: -NECROSIS, PAPILLARY,-MINIMAL, FOCAL LIGHT GREEN, CREAMY SEMIFLUID -PYELONEPHRITIS,-MODERATELY SEVERE, -CALCULUS(I); BILATERAL: MULTIPLE TAN, DIFFUSE HARD CALCULI, UP TO 5 MM IN DIAMETER SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE, MULTI- FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 5 MM IN >UNREMARKABLE DIAMETER TESTIS (TE) : -MINERALIZATION,-SLIGHT, MULTI-FOCAL BONE, FEMUR (FE) : >NOTE:>TRIMMING COMMENT: MISSING AT TRIMMING 2020 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 422 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90872 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/24/99 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/24/99 6:54 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : >NOTE:>TRIMMING COMMENT: MISSING AT TRIMMING HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 5 MM, DARK RED AND -HEMORRHAGE,-MODERATE, MULTI-FOCAL SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -MINERALIZATION,-MODERATE, MULTI-FOCAL URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,- MODERATELY SEVERE, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PYELONEPHRITIS,-PRESENT -------------------------------------------- 2021 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 423 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90872 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/24/99 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/24/99 6:54 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2022 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 424 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90873 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 622.3 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 8:17 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : LEFT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >BEHAVIOR; ATAXIC; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >APPEARANCE; HEAD TILT; RIGHT; VERIFIED -HEMORRHAGE,-SLIGHT, MULTI-FOCAL AT NECROPSY: NOT APPLICABLE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >SKIN & PELAGE; RED HAIR COAT; VENTRAL- FOCAL THORACIC; VERIFIED AT NECROPSY: -NECROSIS, HEPATOCELLULAR,-SLIGHT, OBSERVED MULTI-FOCAL >SKIN & PELAGE; RED HAIR COAT; VENTRAL- KIDNEY (KD) : ABDOMINAL; VERIFIED AT NECROPSY: -CAST, PROTEINACEOUS,-MINIMAL, MULTI- OBSERVED FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 12 X 8 X 4 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, DARK RED, TAN, SOFT AND LOBULATED -B-ADENOMA,-NEOPLASM PRESENT 2023 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 425 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90873 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 622.3 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 8:17 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT: SINGLE RED AND -EROSION,-MODERATE, MULTI-FOCAL DRY AREA, 7 X 5 MM; COLLECTED -FIBROSIS,-MODERATELY SEVERE, MULTI- FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL AND THORACIC REGIONS: RED AND DRY -------------------------------------------- 2024 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 426 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90873 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 622.3 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 8:17 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2025 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 427 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90874 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/03/99 STUDY DAY OF DEATH: 463 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 520.7 GRAMS DATE AND TIME OF NECROPSY: 05/03/99 11:14 PROSECTOR: JOHN S. HALFORD RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR,-MINIMAL, >EYES; RED DISCHARGE; EYES; VERIFIED AT MULTI-FOCAL NECROPSY: OBSERVED KIDNEY (KD) : >RESPIRATION; LABORED; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE MODERATE, MULTI-FOCAL >RESPIRATION; AUDIBLE; VERIFIED AT PANCREAS (PA) : NECROPSY: NOT APPLICABLE -PIGMENT,-MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -MASS(ES); MUCOSA, ALONG GREATER -CYST,-PRESENT CURVATURE: SINGLE TAN AND SOFT MASS, 6 X 5 X 3 MM; ON SECTION EXUDES A WHITE CASEOUS MATERIAL; COLLECTED INTACT; REGIONAL LN (PANCREATIC) NOT IDENTIFIED STOMACH, NONGL (SU) : -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, URETHRA: GREEN AND FIRM MULTI-FOCAL 2026 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 428 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90874 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/03/99 STUDY DAY OF DEATH: 463 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 520.7 GRAMS DATE AND TIME OF NECROPSY: 05/03/99 11:14 PROSECTOR: JOHN S. HALFORD RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -THICKENED; ALL REGIONS DIFFUSE: TAN -PIGMENT,-MINIMAL, MULTI-FOCAL AND FIRM (COLLECTED ON ROUTINE SECTION) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -GI TRACT-LARGE LUMEN; JEJUNUM, ILEUM AND CECUM, DIFFUSE: UP TO 25 MM IN DIAMETER -------------------------------------------- 2027 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 429 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90874 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/03/99 STUDY DAY OF DEATH: 463 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 520.7 GRAMS DATE AND TIME OF NECROPSY: 05/03/99 11:14 PROSECTOR: JOHN S. HALFORD RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2028 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 430 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90875 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/19/99 STUDY DAY OF DEATH: 632 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/19/99 9:50 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; PANCREAS (PA) : VERIFIED AT NECROPSY: OBSERVED -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- SLIGHT, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -ONE EXAMINED,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2029 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 431 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90875 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/19/99 STUDY DAY OF DEATH: 632 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/19/99 9:50 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2030 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 432 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90876 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (A) DATE OF DEATH: 02/15/99 STUDY DAY OF DEATH: 386 STUDY WEEK OF DEATH: 56 TERMINAL BODY WEIGHT: 888.8 GRAMS DATE AND TIME OF NECROPSY: 02/15/99 13:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >APPEARANCE; PARALYSIS; LIMBS-HIND; FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL LUNG (LU) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL MUSCLE, SKELETAL (SM) : MUSCLE, SKELETAL (SM) : -DIFFUSELY RED; MUSCLE SURROUNDING -HEMORRHAGE,-MODERATE, MULTI-FOCAL VENTRAL SURFACE OF MID THORACIC REGION: DARK RED; COLLECTED INTACT WITH SPINAL CORD THYROID (TY) : -CYST, FOLLICULAR,-MINIMAL, MULTI-FOCAL PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT EPIDIDYMIDES (EP) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL 2031 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 433 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90876 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (A) DATE OF DEATH: 02/15/99 STUDY DAY OF DEATH: 386 STUDY WEEK OF DEATH: 56 TERMINAL BODY WEIGHT: 888.8 GRAMS DATE AND TIME OF NECROPSY: 02/15/99 13:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -TRAUMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -------------------------------------------- 2032 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 434 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90876 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (A) DATE OF DEATH: 02/15/99 STUDY DAY OF DEATH: 386 STUDY WEEK OF DEATH: 56 TERMINAL BODY WEIGHT: 888.8 GRAMS DATE AND TIME OF NECROPSY: 02/15/99 13:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2033 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 435 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90877 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 454.9 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:32 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0294 .0065 % .0131 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0220 .0048 % .0098 WEIGHT TAKEN BRAIN (BR) 2.2403 .4925 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.8338 .4031 % .8186 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.7847 .3923 % .7966 WEIGHT TAKEN LIVER (LI) 17.3893 3.8227 % 7.7620 WEIGHT TAKEN LUNG (LU) 1.9130 .4205 % .8539 WEIGHT TAKEN SPLEEN (SP) .5725 .1259 % .2555 WEIGHT TAKEN LEFT TESTIS (TE0) 1.6055 .3529 % .7166 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.6214 .3564 % .7237 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0136 .0030 % .0061 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0150 .0033 % .0067 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: NOT OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT, MULTI- FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN 2034 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 436 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90877 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 454.9 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:32 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS) ------------------------------------------------------------------------------------------------------------------------------------ 2035 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 437 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90878 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/29/99 STUDY DAY OF DEATH: 369 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/29/99 8:35 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; PANCREAS (PA) : VERIFIED AT NECROPSY: OBSERVED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT GENERAL COMMENT (GC) : -AUTOLYSIS-SEVERE -------------------------------------------- 2036 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 438 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90878 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/29/99 STUDY DAY OF DEATH: 369 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/29/99 8:35 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2037 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 439 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90879 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 443.5 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 14:12 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >APPEARANCE; HUNCHED; VERIFIED AT -MOTTLED; ALL LOBES, DIFFUSE: RED AND >UNREMARKABLE NECROPSY: NOT APPLICABLE BROWN KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-SLIGHT, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATELY SEVERE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 11 X 8 X 8 MM, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL FIRM, TAN, RED AND DARK RED -B-ADENOMA,-NEOPLASM PRESENT ILEUM (IL) : >TISSUE MISSING RECTUM (RE) : RECTUM (RE) : >NOTE:>TRIMMING COMMENT: NOT FOUND AT >TISSUE MISSING TRIMMING 2038 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 440 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90879 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 443.5 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 14:12 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST BROWN MATERIAL -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2039 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 441 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90879 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 443.5 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 14:12 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2040 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 442 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90880 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 566.1 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:23 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0324 .0057 % .0145 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0265 .0047 % .0119 WEIGHT TAKEN BRAIN (BR) 2.2340 .3946 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.4180 .4271 % 1.0824 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.3287 .4114 % 1.0424 WEIGHT TAKEN LIVER (LI) 20.5702 3.6337 % 9.2078 WEIGHT TAKEN LUNG (LU) 2.1495 .3797 % .9622 WEIGHT TAKEN SPLEEN (SP) .8744 .1545 % .3914 WEIGHT TAKEN LEFT TESTIS (TE0) 1.6470 .2909 % .7372 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.6603 .2933 % .7432 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0165 .0029 % .0074 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0152 .0027 % .0068 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN 2041 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 443 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90880 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 566.1 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:23 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2042 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 444 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90881 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 978.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:43 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -DEGENERATION, CYSTIC,-MODERATE, MULTI- MULTIPLE DARK RED AREAS, UP TO 2 MM IN FOCAL DIAMETER; COLLECTED ON ROUTINE -FOCUS, ALTERED HEPATOCELLULAR, SECTIONS EOSINOPHILIC,-SEVERE, MULTI-FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 7 X 5 X 4 MM (LEFT IS 4 >UNREMARKABLE X 3 X 3 MM) ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL 2043 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 445 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90881 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 978.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:43 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- TAN, AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 2044 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 446 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90881 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 978.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:43 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2045 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 447 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90882 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 985.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:41 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -DEGENERATION, CYSTIC,-SLIGHT, MULTI- FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, MULTI-FOCAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -CYST(S); MID ANTERIOR ASPECT: SINGLE -B-ADENOMA,-NEOPLASM PRESENT CLEAR FLUID FILLED CYST, 1 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 2046 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 448 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90882 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 985.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:41 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), LUNG (LU), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2047 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 449 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90883 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 621.3 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:29 PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LIVER (LI) : LEFT; VERIFIED AT NECROPSY: NOT -FOCUS, ALTERED HEPATOCELLULAR, OBSERVED EOSINOPHILIC,-MINIMAL, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 5 X 5 MM, DARK RED, TAN -HEMORRHAGE,-MODERATELY SEVERE, MULTI- AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, ACUTE,-SLIGHT, MULTI- URETHRA: GREEN, TAN, AND FIRM FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN (CAP -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 22) 2048 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 450 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90883 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 621.3 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:29 PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), LUNG (LU), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2049 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 451 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90884 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 593.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:48 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : LEFT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- MULTIPLE AREAS, UP TO 2 MM IN FOCAL DIAMETER; COLLECTED ON ROUTINE -TELANGIECTASIS,-MINIMAL, FOCAL SECTIONS KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 4 MM, RED, GRAY, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND FIRM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL -EDEMA,-MODERATE, FOCAL HINDFEET: SINGLE RED, TAN AND MOIST -EROSION,-MODERATELY SEVERE, FOCAL AREA ON EACH, LEFT IS 11 X 10 MM, -EXOSTOSES,-MODERATE, FOCAL RIGHT IS 3 MM IN DIAMETER; BOTH -INFLAMMATION, ACUTE,-MODERATE, FOCAL COLLECTED; TISSUE SURROUNDING LEFT APPEARS SWOLLEN GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN 2050 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 452 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90884 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 593.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:48 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), LUNG (LU), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2051 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 453 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90885 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 751.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:38 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LIVER (LI) : LEFT; VERIFIED AT NECROPSY: OBSERVED -DEGENERATION, CYSTIC,-MODERATE, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL -MINERALIZATION, PELVIC,-MINIMAL, FOCAL LUNG (LU) : LUNG (LU) : -ADHESION(S); LEFT LOBE ADHERED TO -FIBROSIS, PLEURAL/SUBPLEURAL,-MINIMAL, PLEURA: MULTIPLE GRAY AND FIBROUS MULTI-FOCAL ADHESIONS; COLLECTED INTACT THYROID (TY) : THYROID (TY) : -LARGE; RIGHT: 8 X 6 X 6 MM (LEFT IS 4 -B-ADENOMA, C-CELL,-NEOPLASM PRESENT X 3 X 2 MM) URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; LEFT: 12 X 7 X 5 MM (RIGHT IS -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 7 X 3 X 3 MM) -DIFFUSELY RED; LEFT: DARK RED (CAP 22) 2052 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 454 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90885 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 751.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:38 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT -EDEMA,-MODERATE, FOCAL HINDFOOT: SINGLE DARK RED AND TAN -EROSION,-MODERATELY SEVERE, FOCAL ABRADED AREA, 10 X 8 MM; COLLECTED -EXOSTOSES,-MODERATELY SEVERE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2053 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 455 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90886 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 470.7 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 11:38 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; SORE/SCAB; PAW-HIND- -DILATATION, VENTRICULAR,-PRESENT RIGHT; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >WART LIKE LESION(S); PAW-HIND-LEFT; -RED FOCUS(I)/AREA(S); ALL LOBES: -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- WEEK 89; VERIFIED AT NECROPSY: NOT MULTIPLE DARK RED AREAS, 3 MM IN FOCAL OBSERVED DIAMETER; COLLECTED ON ROUTINE >SKIN & PELAGE; PALE; ENTIRE BODY; SECTIONS VERIFIED AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >EXCRETION; FEW FECES; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE MINIMAL, MULTI-FOCAL >EYES; SQUINTED; PERI-ORBITAL; VERIFIED ADRENAL, CORTEX (AC) : AT NECROPSY: NOT APPLICABLE -VACUOLATION,-MINIMAL, MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 MM IN DIAMETER, RED, TAN -HEMORRHAGE,-MODERATE, MULTI-FOCAL AND SOFT -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, URETHRA: GREEN AND FIRM MULTI-FOCAL 2054 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 456 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90886 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 470.7 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 11:38 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT TAIL (TI) : TAIL (TI) : -CRUSTED AREA(S); DIFFUSE ALONG ENTIRE -CYST, EPIDERMAL INCLUSION,-PRESENT LENGTH: MULTIPLE DARK BROWN AND SLIGHTLY RAISED AREAS, UP TO 4 MM IN DIAMETER; COLLECTED FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); RIGHT HIND -EROSION,-MODERATELY SEVERE, FOCAL FOOT: SINGLE BROWN, RED, AND MOIST -FIBROSIS,-MODERATELY SEVERE, MULTI- ABRADED AREA, 12 MM IN DIAMETER; FOCAL COLLECTED -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL LN, POPLITEAL (PP) : LN, POPLITEAL (PP) : -LARGE; RIGHT: UP TO 11 X 10 X 6 MM -DILATATION, SINUSOIDAL,-SLIGHT, MULTI- (LEFT IS NOT IDENTIFIED) (CAP 22) FOCAL -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, MULTI-FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; COVERING ENTIRE ABDOMINAL REGION: DRY BROWN MATERIAL -BLOOD FILM TAKEN -------------------------------------------- 2055 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 457 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90886 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 470.7 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 11:38 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2056 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 458 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90887 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 701.2 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 15:47 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; VENTRAL- BRAIN (BR) : BRAIN (BR) : ABDOMINAL; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; RED HAIR COAT; HEAD- LIVER (LI) : LIVER (LI) : ENTIRE; VERIFIED AT NECROPSY: OBSERVED -MOTTLED; ALL LOBES: RED AND TAN -VACUOLATION, HEPATOCELLULAR,-SEVERE, >EXCRETION; NO FECES; VERIFIED AT DIFFUSE NECROPSY: NOT APPLICABLE KIDNEY (KD) : >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -NEPHROPATHY, CHRONIC PROGRESSIVE,- AT NECROPSY: NOT APPLICABLE MINIMAL, MULTI-FOCAL >SKIN & PELAGE; RED HAIR COAT; VENTRAL- PANCREAS (PA) : CERVICAL; VERIFIED AT NECROPSY: -ATROPHY, ACINAR,-SLIGHT, FOCAL OBSERVED -PIGMENT,-MINIMAL, MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT HEART (HT) : NECROPSY: NOT APPLICABLE -DEGENERATION/NECROSIS,-SLIGHT, MULTI- >APPEARANCE; HUNCHED; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 6 X 9 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL SOFT, RED AND DARK RED -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -EDEMA,-SLIGHT, MULTI-FOCAL TAN AND BROWN ERODED AREAS, UP TO 3 MM -EROSION,-MODERATE, FOCAL IN DIAMETER -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL 2057 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 459 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90887 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 701.2 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 15:47 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; DRY RED MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE AND HEAD: DRY RED MATERIAL -------------------------------------------- 2058 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 460 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90887 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 701.2 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 15:47 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2059 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 461 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90888 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 433.7 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 14:46 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >WART LIKE LESION(S); DORSAL; WEEK 79; -DILATATION, VENTRICULAR,-PRESENT VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -MOTTLED; ALL LOBES: TAN AND BROWN -VACUOLATION, HEPATOCELLULAR,- NECROPSY: OBSERVED MODERATELY SEVERE, MULTI-FOCAL >EXCRETION; NO FECES; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MINIMAL, FOCAL >SKIN & PELAGE; RED HAIR COAT; PERINEAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- AREA; VERIFIED AT NECROPSY: OBSERVED MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 7 X 12 MM, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- FIRM, RED, DARK RED AND TAN FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: SINGLE -EDEMA,-MINIMAL, FOCAL DARK BROWN ERODED AREA, 2 X 3 MM -EROSION,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI- FOCAL STOMACH, NONGL (SU) : -EDEMA,-MODERATE, MULTI-FOCAL 2060 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 462 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90888 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 433.7 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 14:46 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE -EROSION,-MODERATE, FOCAL DARK BROWN ERODED AREA, 2 X 4 MM -INFLAMMATION, ACUTE,-MINIMAL, MULTI- FOCAL SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); DORSAL THORACIC -B-KERATOACANTHOMA,-NEOPLASM PRESENT REGION: SINGLE DRY, TAN AREA, 5 X 6 MM (CAP 21) PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,- URETHRA: MULTIPLE TAN AND GREEN MODERATELY SEVERE, MULTI-FOCAL NODULES CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DARK BROWN MOIST MATERIAL -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -ANIMAL THIN -------------------------------------------- 2061 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 463 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90888 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 433.7 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 14:46 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2062 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 464 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90889 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 818.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:03 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LIVER (LI) : LEFT; VERIFIED AT NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL THYROID (TY) : THYROID (TY) : -LARGE; RIGHT: 9 X 6 X 6 MM (LEFT IS 5 -CYST, ULTIMOBRANCHIAL,-PRESENT X 3 X 3 MM) -B-ADENOMA, C-CELL,-NEOPLASM PRESENT SKIN (SK) : SKIN (SK) : -MASS(ES); LEFT LATERAL HINDLEG: -CYST, EPIDERMAL INCLUSION,-PRESENT SINGLE MASS, 15 X 10 X 5 MM, TAN AND -B-KERATOACANTHOMA,-NEOPLASM PRESENT SOFT; ON SECTION EXUDES A TAN CASEOUS SEMISOLID MATERIAL (CAP 23); REGIONAL LN (INGUINAL) NOT IDENTIFIED -CRUSTED AREA(S); LEFT LATERAL LUMBAR REGION: SINGLE DRY DARK RED AREA, 5 MM IN DIAMETER (CAP 22) URINARY BLADDER (UB) : -CALCULUS,-PRESENT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, URETHRA: GREEN AND FIRM MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2063 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 465 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90889 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 818.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:03 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT -EDEMA,-MODERATE, FOCAL HINDFOOT: SINGLE DARK RED ABRADED -EROSION,-SLIGHT, FOCAL AREA, 10 X 8 MM; COLLECTED -FIBROSIS,-SLIGHT, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), LUNG (LU) ------------------------------------------------------------------------------------------------------------------------------------ 2064 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 466 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90890 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 360.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:45 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 11.2612 3.1212 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2065 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 467 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90890 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 360.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:45 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2066 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 468 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90891 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 372.6 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:45 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 13.2084 3.5449 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2067 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 469 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90891 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 372.6 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:45 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2068 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 470 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90892 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 419.6 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:09 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; NO FECES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -VACUOLATION, HEPATOCELLULAR,-MODERATE, NECROPSY: OBSERVED MULTI-FOCAL >SKIN & PELAGE; RED HAIR COAT; HEAD- KIDNEY (KD) : ENTIRE; VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >EYES; SQUINTED; PERI-ORBITAL-RIGHT; FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE LUNG (LU) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE THYROID (TY) : THYROID (TY) : >NOTE:>TRIMMING COMMENT: ONE MISSING -ONE EXAMINED,-PRESENT FROM WET TISSUE PARATHYROID (PT) : >NOTE:>TRIMMING COMMENT: ONE MISSING FROM WET TISSUE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 5 X 7 X 9 MM, SOFT, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED AND TAN -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2069 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 471 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90892 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 419.6 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:09 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); BILATERAL, CERVICAL REGIONS: -GALACTOCELE,-MODERATE, MULTI-FOCAL MULTIPLE WHITE FLUID FILLED CYSTS, UP TO 2 MM IN DIAMETER (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -DISCHARGE-NASAL; DRY, RED MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: RED -ANIMAL THIN -------------------------------------------- 2070 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 472 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90892 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 419.6 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:09 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2071 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 473 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90893 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 908.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:16 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -MOTTLED; ALL LOBES: DARK RED, TAN, -DEGENERATION, CYSTIC,-MODERATELY AND BROWN SEVERE, MULTI-FOCAL -RED FOCUS(I)/AREA(S); ALL LOBES: -FOCUS, ALTERED HEPATOCELLULAR, MULTIPLE DARK RED AREAS, UP TO 3 MM IN EOSINOPHILIC,-MODERATE, MULTI-FOCAL DIAMETER; COLLECTED ON ROUTINE -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- SECTIONS FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -RAISED AREA(S); LEFT POSTERIOR -HEMORRHAGE,-MODERATE, MULTI-FOCAL ASPECT: SINGLE DARK RED AREA, 3 MM IN -B-ADENOMA,-NEOPLASM PRESENT DIAMETER PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, ACUTE,-SLIGHT, MULTI- URETHRA: GREEN AND FIRM FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN 2072 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 474 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90893 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 908.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:16 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), LUNG (LU), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2073 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 475 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90894 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/10/99 STUDY DAY OF DEATH: 684 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 655.4 GRAMS DATE AND TIME OF NECROPSY: 12/10/99 14:02 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; BROWN HAIR COAT; KIDNEY (KD) : PERINEAL AREA; VERIFIED AT NECROPSY: -CAST, PROTEINACEOUS,-MINIMAL, MULTI- OBSERVED FOCAL >SKIN & PELAGE; RED HAIR COAT; HEAD- ADRENAL, CORTEX (AC) : ENTIRE; VERIFIED AT NECROPSY: OBSERVED -VACUOLATION,-SLIGHT, MULTI-FOCAL >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED MUSCLE, SKELETAL (SM) : AT NECROPSY: NOT APPLICABLE -DEGENERATION/NECROSIS,-MINIMAL, MULTI- >RESPIRATION; LABORED; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE THYROID (TY) : THYROID (TY) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -RED FOCUS(I)/AREA(S); RIGHT: SINGLE -PIGMENT,-SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE DARK RED AREA, 2 MM IN DIAMETER -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EDEMA,-MINIMAL, FOCAL MULTIPLE DARK BROWN AREAS, UP TO 3 MM -EROSION,-MODERATE, FOCAL IN DIAMETER -HEMORRHAGE,-SLIGHT, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL PROSTATE (PR) : -MINERALIZATION, LUMINAL,-MODERATE, MULTI-FOCAL 2074 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 476 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90894 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/10/99 STUDY DAY OF DEATH: 684 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 655.4 GRAMS DATE AND TIME OF NECROPSY: 12/10/99 14:02 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ EPIDIDYMIDES (EP) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -CARCINOMA,-PRESENT LN, MEDIASTINAL (ML) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MODERATE, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS ON THYMUS SECTION CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -MASS(ES); WITHIN MESENTERY, ADJACENT -M-CARCINOMA,-NEOPLASM PRESENT TO COLON: SINGLE MASS, 35 X 25 X 20 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A CLEAR RED FLUID; COLLECTED INTACT; REGIONAL LN (MESENTERIC) NORMAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; PERINEUM: MOIST BROWN MATERIAL -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; VENTRAL CERVICAL REGION: DRY DARK RED MATERIAL -------------------------------------------- 2075 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 477 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90894 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/10/99 STUDY DAY OF DEATH: 684 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 655.4 GRAMS DATE AND TIME OF NECROPSY: 12/10/99 14:02 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2076 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 478 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90895 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/13/99 STUDY DAY OF DEATH: 657 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/13/99 7:35 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: NOT OBSERVED -LARGE; ALL LOBES -HEMORRHAGE,-MODERATE, MULTI-FOCAL -MOTTLED; ALL LOBES: RED, DARK RED, -INFLAMMATION, CHRONIC-ACTIVE,- TAN AND YELLOW MODERATE, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-SEVERE, MULTI-FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LUNG (LU) : LUNG (LU) : -MOTTLED; ALL LOBES: RED, DARK RED AND -HEMORRHAGE,-SEVERE, MULTI-FOCAL TAN -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO -UNCOLLAPSED; ALL LOBES NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC 2077 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 479 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90895 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/13/99 STUDY DAY OF DEATH: 657 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/13/99 7:35 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -HISTIOCYTIC SARCOMA,-PRESENT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT GENERAL COMMENT (GC) : -DISCHARGE-ORAL; DRY, DARK RED MATERIAL -------------------------------------------- 2078 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 480 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90895 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/13/99 STUDY DAY OF DEATH: 657 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/13/99 7:35 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) ------------------------------------------------------------------------------------------------------------------------------------ 2079 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 481 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90896 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/07/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 489.3 GRAMS DATE AND TIME OF NECROPSY: 06/07/99 14:54 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >APPEARANCE; HUNCHED; VERIFIED AT -TELANGIECTASIS,-MODERATE, FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >APPEARANCE; THIN; VERIFIED AT -CAST, PROTEINACEOUS,-SLIGHT, MULTI- NECROPSY: NOT OBSERVED FOCAL >EXCRETION; FEW FECES; VERIFIED AT PITUITARY (PI) : PITUITARY (PI) : NECROPSY: NOT APPLICABLE -MASS(ES); SINGLE MASS, 13 X 10 X 7 -HEMORRHAGE,-MODERATE, MULTI-FOCAL MM, DARK RED, RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 30 X 20 X -SECRETION, INCREASED,-PRESENT 10 MM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS 2080 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 482 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90896 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/07/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 489.3 GRAMS DATE AND TIME OF NECROPSY: 06/07/99 14:54 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2081 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 483 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90897 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/17/99 STUDY DAY OF DEATH: 661 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/17/99 7:30 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -DEGENERATION, CYSTIC,-MODERATE, MULTI- MULTIPLE DARK RED AREAS, UP TO 3 X 2 FOCAL MM; COLLECTED ON ROUTINE SECTIONS -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- SLIGHT, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI- FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); DIFFUSE: -HEMORRHAGE,-MODERATE, FOCAL MULTIPLE DARK RED FOCI, PINPOINT AND -B-ADENOMA,-NEOPLASM PRESENT UP TO 2 MM IN DIAMETER PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, URETHRA: GREEN AND FIRM MULTI-FOCAL 2082 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 484 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90897 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/17/99 STUDY DAY OF DEATH: 661 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/17/99 7:30 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -HEPATOCELLULAR ADENOMA,-PRESENT -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2083 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 485 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90898 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/01/99 STUDY DAY OF DEATH: 431 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 475.4 GRAMS DATE AND TIME OF NECROPSY: 04/01/99 10:09 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; RED HAIR COAT; HEAD- -DILATATION, VENTRICULAR,-PRESENT ENTIRE; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -VACUOLATION, HEPATOCELLULAR,- AT NECROPSY: NOT APPLICABLE MODERATELY SEVERE, MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EXCRETION; NO FECES; VERIFIED AT MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE ADRENAL, MEDULLA (MA) : >SKIN & PELAGE; PALE; ENTIRE BODY; -ONE EXAMINED,-PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 6 X 9 MM, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- SOFT, RED, TAN AND BROWN FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL 2084 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 486 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90898 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/01/99 STUDY DAY OF DEATH: 431 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 475.4 GRAMS DATE AND TIME OF NECROPSY: 04/01/99 10:09 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 2085 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 487 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90898 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/01/99 STUDY DAY OF DEATH: 431 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 475.4 GRAMS DATE AND TIME OF NECROPSY: 04/01/99 10:09 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2086 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 488 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90899 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 478.0 GRAMS DATE AND TIME OF NECROPSY: 01/17/00 8:29 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; RED HAIR COAT; VENTRAL- -DILATATION, VENTRICULAR,-PRESENT ABDOMINAL; VERIFIED AT NECROPSY: LIVER (LI) : LIVER (LI) : OBSERVED -MASS(ES); NEAR HILUS OF PAPILLARY -DEGENERATION, CYSTIC,-SLIGHT, FOCAL >EYES; RED DISCHARGE; EYES; VERIFIED AT PROCESS: SINGLE MASS, 21 X 16 X 10 MM, -VACUOLATION, HEPATOCELLULAR,-MODERATE, NECROPSY: OBSERVED RED AND FIRM; SAME ON CUT SURFACE; MULTI-FOCAL >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED COLLECTED INTACT; REGIONAL LN AT NECROPSY: NOT APPLICABLE (HEPATIC) NOT IDENTIFIED >BEHAVIOR; HYPOACTIVE; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MODERATE, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 MM IN DIAMETER, RED, -HEMORRHAGE,-SEVERE, MULTI-FOCAL DARK RED, TAN AND FIRM -B-ADENOMA,-NEOPLASM PRESENT 2087 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 489 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90899 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 478.0 GRAMS DATE AND TIME OF NECROPSY: 01/17/00 8:29 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL STOMACH, NONGL (SU) : -EDEMA,-MINIMAL, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -------------------------------------------- 2088 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 490 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90899 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 478.0 GRAMS DATE AND TIME OF NECROPSY: 01/17/00 8:29 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2089 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 491 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90900 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 655.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:01 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -FIBROSIS,-MINIMAL, FOCAL MULTIPLE DARK RED AREAS, UP TO 4 MM IN -FOCUS, ALTERED HEPATOCELLULAR, DIAMETER; COLLECTED ON ROUTINE EOSINOPHILIC,-MINIMAL, FOCAL SECTIONS -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -PIGMENT,-SLIGHT, FOCAL KIDNEY (KD) : -CYST,-MODERATE, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL TESTIS (TE) : TESTIS (TE) : -LARGE; RIGHT: 26 X 18 X 15 MM (LEFT -ATROPHY/DEGENERATION,-MODERATE, MULTI- IS 20 X 15 X 13 MM) FOCAL -RED/YELLOW FOCUS(I)/AREA(S); RIGHT, -INFLAMMATION, CHRONIC,-MODERATE, MULTI- BENEATH TUNICA: DIFFUSE FOCAL -MINERALIZATION,-SLIGHT, MULTI-FOCAL -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE, FOCAL 2090 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 492 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90900 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 655.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:01 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA) ------------------------------------------------------------------------------------------------------------------------------------ 2091 Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Author: Peter J. Thomford, PhD Study Completion Date: December 13, 2001 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-212 Sponsor Study Identification: 3M T-6316.1 Volume VI of XII Page 2092 of 4873 Covance 6329-212 3M T-6316.1 VOLUME VI OF XII CONTENTS Page APPENDIX 6 (Continued) ........................................................................................ 2094 Individual Anatomic Pathology Data ..................................................................... 2095 2093 APPENDIX 6 (Continued) Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 2094 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 493 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90901 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/21/99 8:14 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; HUNCHED; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LIVER (LI) : -NECROSIS, HEPATOCELLULAR,-SLIGHT, FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 10 X 8 MM, RED, TAN, -HEMORRHAGE,-MODERATE, MULTI-FOCAL DARK RED AND FIRM -B-ADENOMA,-NEOPLASM PRESENT 2095 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 494 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90901 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/21/99 8:14 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EROSION,-MODERATE, FOCAL MULTIPLE DARK BROWN LINEAR AREAS, UP -PIGMENT,-MINIMAL, FOCAL TO 3 X 2 MM PROSTATE (PR) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL -MINERALIZATION, LUMINAL,-MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT -------------------------------------------- 2096 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 495 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90901 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/21/99 8:14 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2097 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 496 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90902 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 625.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:34 PROSECTOR: KIMBERLY DURLAND RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0261 .0042 % .0106 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0207 .0033 % .0084 WEIGHT TAKEN BRAIN (BR) 2.4596 .3932 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.0836 .3331 % .8471 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.0882 .3338 % .8490 WEIGHT TAKEN LIVER (LI) 25.2925 4.0429 % 10.2832 WEIGHT TAKEN LUNG (LU) 1.9060 .3047 % .7749 WEIGHT TAKEN SPLEEN (SP) .7737 .1237 % .3146 WEIGHT TAKEN LEFT TESTIS (TE0) 1.6258 .2599 % .6610 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.7143 .2740 % .6970 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0259 .0041 % .0105 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0204 .0033 % .0083 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN 2098 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 497 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90902 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 625.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:34 PROSECTOR: KIMBERLY DURLAND RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2099 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 498 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90903 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 703 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/29/99 12:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: NOT OBSERVED -DEGENERATION, CYSTIC,-SLIGHT, MULTI- FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -FIBROSIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -HEMORRHAGE,-SLIGHT, FOCAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -CYST,-PRESENT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 2100 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 499 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90903 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 703 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/29/99 12:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BLACK FOCI, PINPOINT AND UP TO 1 MM IN DIAMETER PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; -HEMORRHAGE,-MINIMAL, MULTI-FOCAL COLLECTED -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -DISCHARGE-NASAL; RED AND MOIST -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: MOIST AND YELLOW -------------------------------------------- 2101 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 500 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90903 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 703 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/29/99 12:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2102 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 501 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90904 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 572.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:55 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >DISCHARGE; NASAL; RED IN COLOR; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: NOT OBSERVED -MASS(ES); ATTACHED TO VISCERAL -PIGMENT,-SLIGHT, MULTI-FOCAL SURFACE OF RIGHT MEDIAN LOBE: SINGLE -VACUOLATION, HEPATOCELLULAR,- MASS, 17 X 15 X 6 MM, TAN, RED, MODERATELY SEVERE, MULTI-FOCAL VASCULAR AND SOFT; SAME ON CUT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM SURFACE; COLLECTED ON ROUTINE SECTION; PRESENT REGIONAL LN (HEPATIC) NOT IDENTIFIED LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,- MODERATELY SEVERE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT 2103 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 502 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90904 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 572.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:55 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; RIGHT: RED AND TAN; -HEMORRHAGE,-MINIMAL, MULTI-FOCAL COLLECTED LN, MEDIASTINAL (ML) : -DILATATION, SINUSOIDAL,-SLIGHT, FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2104 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 503 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90904 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 572.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:55 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2105 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 504 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90905 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 420.9 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:32 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0327 .0078 % .0149 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0281 .0067 % .0128 WEIGHT TAKEN BRAIN (BR) 2.1911 .5206 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.9594 .4655 % .8943 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.9588 .4654 % .8940 WEIGHT TAKEN LIVER (LI) 21.7331 5.1635 % 9.9188 WEIGHT TAKEN LUNG (LU) 1.6862 .4006 % .7696 WEIGHT TAKEN SPLEEN (SP) .6461 .1535 % .2949 WEIGHT TAKEN LEFT TESTIS (TE0) 1.5387 .3656 % .7023 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.4719 .3497 % .6718 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0141 .0033 % .0064 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0111 .0026 % .0051 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, DIFFUSE ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2106 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 505 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90905 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 420.9 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:32 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2107 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 506 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90906 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/08/00 STUDY DAY OF DEATH: 713 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 578.4 GRAMS DATE AND TIME OF NECROPSY: 01/08/00 8:09 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; BROWN HAIR COAT; -DILATATION, VENTRICULAR,-PRESENT VENTRAL-ABDOMINAL; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -MOTTLED; ALL LOBES: DARK RED AND TAN -VACUOLATION, HEPATOCELLULAR,-SEVERE, >BEHAVIOR; HYPOACTIVE; VERIFIED AT DIFFUSE NECROPSY: NOT APPLICABLE KIDNEY (KD) : KIDNEY (KD) : >APPEARANCE; THIN; VERIFIED AT -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT OBSERVED -MOTTLED; BILATERAL: DARK RED AND TAN MODERATELY SEVERE, DIFFUSE >EXCRETION; NO FECES; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: NOT APPLICABLE -ANGIECTASIS,-MODERATE, MULTI-FOCAL >APPEARANCE; HUNCHED; VERIFIED AT -HEMORRHAGE,-MODERATE, MULTI-FOCAL NECROPSY: NOT APPLICABLE -VACUOLATION,-MODERATE, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL MUSCLE, SKELETAL (SM) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI- FOCAL THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 8 X 4 MM, DARK RED, TAN -HEMORRHAGE,-MODERATE, MULTI-FOCAL AND SOFT -B-ADENOMA,-NEOPLASM PRESENT 2108 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 507 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90906 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/08/00 STUDY DAY OF DEATH: 713 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 578.4 GRAMS DATE AND TIME OF NECROPSY: 01/08/00 8:09 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : -HYPERPLASIA, EPITHELIAL,-MODERATE, DIFFUSE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: BROWN -------------------------------------------- 2109 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 508 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90906 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/08/00 STUDY DAY OF DEATH: 713 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 578.4 GRAMS DATE AND TIME OF NECROPSY: 01/08/00 8:09 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2110 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 509 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90907 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 449.3 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:46 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0302 .0067 % .0137 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0300 .0067 % .0137 WEIGHT TAKEN BRAIN (BR) 2.1970 .4890 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.9941 .4438 % .9076 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.9800 .4407 % .9012 WEIGHT TAKEN LIVER (LI) 21.0493 4.6849 % 9.5809 WEIGHT TAKEN LUNG (LU) 1.7525 .3901 % .7977 WEIGHT TAKEN SPLEEN (SP) .6630 .1476 % .3018 WEIGHT TAKEN LEFT TESTIS (TE0) 1.7456 .3885 % .7945 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.8060 .4020 % .8220 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0156 .0035 % .0071 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0121 .0027 % .0055 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2111 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 510 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90907 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 449.3 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:46 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2112 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 511 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90908 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 786.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:49 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : NECROPSY: OBSERVED -B-GRANULAR CELL TUMOR,-NEOPLASM PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-MODERATE, MULTI-FOCAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT TESTIS (TE) : TESTIS (TE) : -RED FOCUS(I)/AREA(S); LEFT, BENEATH -HEMORRHAGE,-MODERATE, MULTI-FOCAL TUNICA: SINGLE DARK RED AREA, 7 X 6 MM -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT 2113 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 512 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90908 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 786.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:49 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -LIGHT FOCUS(I)/AREA(S); RIGHT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL POSTERIOR ASPECT: SINGLE TAN AREA, 2 -B-ADENOMA,-NEOPLASM PRESENT MM IN DIAMETER RECTUM (RE) : -PARASITISM,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2114 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 513 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90908 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 786.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:49 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2115 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 514 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90909 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 790.3 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:45 PROSECTOR: DONIA MEYER RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0337 .0043 % .0139 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0281 .0036 % .0116 WEIGHT TAKEN BRAIN (BR) 2.4329 .3078 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 3.0718 .3887 % 1.2626 WEIGHT TAKEN RIGHT KIDNEY (KD1) 3.1441 .3978 % 1.2923 WEIGHT TAKEN LIVER (LI) 37.4539 4.7392 % 15.3948 WEIGHT TAKEN LUNG (LU) 2.7025 .3420 % 1.1108 WEIGHT TAKEN SPLEEN (SP) 1.2044 .1524 % .4950 WEIGHT TAKEN LEFT TESTIS (TE0) 1.8463 .2336 % .7589 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.8493 .2340 % .7601 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0277 .0035 % .0114 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0393 .0050 % .0162 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL PANCREAS (PA) : -FIBROSIS,-MINIMAL, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, FOCAL 2116 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 515 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90909 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 790.3 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:45 PROSECTOR: DONIA MEYER RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HYPERPLASIA,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2117 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 516 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90909 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 790.3 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:45 PROSECTOR: DONIA MEYER RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2118 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 517 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90910 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 809.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:46 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); LEFT LATERAL -DEGENERATION, CYSTIC,-MINIMAL, FOCAL LOBE: MULTIPLE DARK RED AREAS, UP TO 3 -HYPERPLASIA, BILE DUCT,-SLIGHT, MULTI- MM IN DIAMETER; COLLECTED ON ROUTINE FOCAL SECTION -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATELY SEVERE, FOCAL ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-SLIGHT, FOCAL -VACUOLATION,-SLIGHT, FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 2119 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 518 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90910 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 809.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:46 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -LIGHT FOCUS(I)/AREA(S); MID ASPECT: -B-ADENOMA,-NEOPLASM PRESENT SINGLE GRAY AREA, 3 MM IN DIAMETER PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2120 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 519 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90910 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 809.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:46 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2121 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 520 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90911 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 719.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:53 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); MOUTH; WEEK 95; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); RIGHT MEDIAN -DEGENERATION, CYSTIC,-MINIMAL, MULTI- LOBE: SINGLE TAN AREA, 3 MM IN FOCAL DIAMETER; COLLECTED ON ROUTINE SECTION -HYPERTROPHY, CENTRILOBULAR, -LARGE; ALL LOBES HEPATOCELLULAR,-MODERATELY SEVERE, -DARK FOCUS(I)/AREA(S); LEFT LATERAL DIFFUSE LOBE: SINGLE DARK BROWN AREA, 3 MM IN -LIPIDOSIS, SUBCAPSULAR (TENSION DIAMETER; COLLECTED ON ROUTINE SECTION LIPIDOSIS),-MODERATELY SEVERE, FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : KIDNEY (KD) : -DIFFUSELY DARK; BILATERAL: DARK BROWN >UNREMARKABLE PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -DIFFUSELY DARK; BILATERAL: DARK BROWN -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -VACUOLATION,-SLIGHT, FOCAL TESTIS (TE) : -MINERALIZATION, VASCULAR,-SLIGHT, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, FOCAL 2122 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 521 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90911 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 719.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:53 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -CYST, FOLLICULAR,-MODERATELY SEVERE, FOCAL -HYPERPLASIA, FOLLICULAR CELL,- MODERATE, FOCAL RECTUM (RE) : -PARASITISM,-PRESENT SKIN (SK) : SKIN (SK) : -RAISED AREA(S); LEFT UPPER LIP: -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM SINGLE GRAY, RAISED AREA, 3 MM IN PRESENT DIAMETER (CAP 22); POSSIBLY CONSISTENT WITH WART-LIKE LESION (MOUTH) AS NOTED IN CLINICAL OBSERVATIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 2123 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 522 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90911 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 719.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:53 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2124 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 523 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90912 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 568 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 403.4 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 13:25 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -DILATATION, VENTRICULAR,-PRESENT AT NECROPSY: NOT APPLICABLE LIVER (LI) : >APPEARANCE; HUNCHED; VERIFIED AT -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >BEHAVIOR; ATAXIC; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >SKIN & PELAGE; PALE; ENTIRE BODY; MINIMAL, MULTI-FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE -PYELONEPHRITIS,-SLIGHT, FOCAL >SKIN & PELAGE; YELLOW HAIR COAT; ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : VERIFIED AT NECROPSY: NOT OBSERVED -SMALL; BILATERAL: EACH IS 4 X 3 X 2 >UNREMARKABLE MM TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 18 X 10 X 5 -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE MM; BOTH ARE SOFT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, MULTI-FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, FOCAL 2125 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 524 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90912 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 568 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 403.4 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 13:25 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 6 MM, TAN AND -B-ADENOMA,-NEOPLASM PRESENT SOFT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL PROSTATE (PR) : PROSTATE (PR) : -SMALL; 10 X 8 X 5 MM -INFLAMMATION, ACUTE,-MINIMAL, MULTI- FOCAL -MINERALIZATION, LUMINAL,-MODERATE, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 6 X 3 >UNREMARKABLE MM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -PIGMENT,-MINIMAL, MULTI-FOCAL TAIL (TI) : TAIL (TI) : -CRUSTED AREA(S); ALONG ENTIRE TAIL: -CYST, EPIDERMAL INCLUSION,-PRESENT MULTIPLE DRY AND BROWN CRUSTED AREAS, -INFLAMMATION, ACUTE,-MODERATE, MULTI- UP TO 5 X 3 MM; COLLECTED FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2126 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 525 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90912 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 568 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 403.4 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 13:25 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2127 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 526 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90913 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 607.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:46 PROSECTOR: GREGORY CODDINGTON RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0320 .0053 % .0145 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0270 .0044 % .0122 WEIGHT TAKEN BRAIN (BR) 2.2108 .3637 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.0030 .3295 % .9060 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.9603 .3225 % .8867 WEIGHT TAKEN LIVER (LI) 30.9958 5.0988 % 14.0202 WEIGHT TAKEN LUNG (LU) 2.0592 .3387 % .9314 WEIGHT TAKEN SPLEEN (SP) .7228 .1189 % .3269 WEIGHT TAKEN LEFT TESTIS (TE0) 1.6724 .2751 % .7565 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.7264 .2840 % .7809 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0256 .0042 % .0116 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0249 .0041 % .0113 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, >APPEARANCE; MISSING; TEETH; VERIFIED HEPATOCELLULAR,-MODERATELY SEVERE, AT NECROPSY: OBSERVED MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HYPERPLASIA,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2128 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 527 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90913 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 607.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:46 PROSECTOR: GREGORY CODDINGTON RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; UPPER RIGHT INCISOR -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; UPPER LEFT INCISOR MISSING -------------------------------------------- 2129 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 528 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90913 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 607.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:46 PROSECTOR: GREGORY CODDINGTON RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2130 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 529 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90914 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 696 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 655.2 GRAMS DATE AND TIME OF NECROPSY: 12/22/99 14:32 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); LIMB-HIND-LEFT; WEEK 76; 65 BRAIN (BR) : BRAIN (BR) : X 60MM; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; RED HAIR COAT; HEAD- -DILATATION, VENTRICULAR,-PRESENT ENTIRE; VERIFIED AT NECROPSY: NOT LIVER (LI) : LIVER (LI) : OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- >SKIN & PELAGE; RED HAIR COAT; VENTRAL- MULTIPLE DARK RED AREAS, UP TO 5 MM IN FOCAL ABDOMINAL; VERIFIED AT NECROPSY: NOT DIAMETER; COLLECTED ON ROUTINE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- OBSERVED SECTIONS FOCAL >APPEARANCE; HUNCHED; VERIFIED AT -VACUOLATION, HEPATOCELLULAR,-MODERATE, NECROPSY: NOT APPLICABLE MULTI-FOCAL >EXCRETION; FEW FECES; VERIFIED AT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM NECROPSY: NOT APPLICABLE PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYMUS (TH) : -CYST,-PRESENT THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 2131 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 530 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90914 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 696 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 655.2 GRAMS DATE AND TIME OF NECROPSY: 12/22/99 14:32 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PARATHYROID (PT) : PARATHYROID (PT) : -LARGE; RIGHT: 5 MM IN DIAMETER (LEFT >UNREMARKABLE IS 1 MM IN DIAMETER) PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 6 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -B-LIPOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 30 X 20 X -SECRETION, INCREASED,-PRESENT 12 MM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); LEFT LATERAL LUMBAR REGION: >UNREMARKABLE SINGLE MASS, 20 X 20 X 10 MM, GRAY AND >NOTE:>MAMMARY MASS DESCRIBED GROSSLY FIRM; SAME ON CUT SURFACE; COLLECTED; WAS A SUBCUTANEOUS LIPOMA REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL OBESE -------------------------------------------- 2132 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 531 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90914 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 696 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 655.2 GRAMS DATE AND TIME OF NECROPSY: 12/22/99 14:32 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2133 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 532 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90915 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 13:03 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >DISCHARGE; NASAL; RED IN COLOR; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL THYMUS (TH) : THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT >SECTION EXAMINED; TISSUE NOT PRESENT POSITIVELY IDENTIFIED; POSSIBLE REMNANTS COLLECTED PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >AUTOLYTIC STOMACH, NONGL (SU) : >AUTOLYTIC DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC 2134 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 533 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90915 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 13:03 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-NASAL; DARK RED AND DRY -AUTOLYSIS-SEVERE; ENTIRE ANIMAL -------------------------------------------- 2135 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 534 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90915 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 13:03 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2136 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 535 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90916 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 723.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 12:53 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: NOT OBSERVED -LARGE; ALL LOBES -HYPERTROPHY, CENTRILOBULAR, -MOTTLED; ALL LOBES: RED AND TAN HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, MULTI-FOCAL -HYPERPLASIA, FOLLICULAR CELL,-SLIGHT, FOCAL RECTUM (RE) : -PARASITISM,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 2137 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 536 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90916 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 723.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 12:53 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2138 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 537 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90917 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 495.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:07 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0301 .0061 % .0134 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0250 .0050 % .0112 WEIGHT TAKEN BRAIN (BR) 2.2389 .4518 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.2821 .4606 % 1.0193 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.2453 .4531 % 1.0029 WEIGHT TAKEN LIVER (LI) 24.6177 4.9683 % 10.9954 WEIGHT TAKEN LUNG (LU) 1.5560 .3140 % .6950 WEIGHT TAKEN SPLEEN (SP) .7012 .1415 % .3132 WEIGHT TAKEN LEFT TESTIS (TE0) 1.8522 .3738 % .8273 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.8916 .3818 % .8449 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0135 .0027 % .0060 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0120 .0024 % .0054 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2139 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 538 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90917 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 495.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:07 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2140 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 539 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90918 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 669.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:32 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0390 .0058 % .0156 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0325 .0049 % .0130 WEIGHT TAKEN BRAIN (BR) 2.4945 .3724 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.4369 .3638 % .9769 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.5132 .3752 % 1.0075 WEIGHT TAKEN LIVER (LI) 29.5214 4.4068 % 11.8346 WEIGHT TAKEN LUNG (LU) 1.8496 .2761 % .7415 WEIGHT TAKEN SPLEEN (SP) .6504 .0971 % .2607 WEIGHT TAKEN LEFT TESTIS (TE0) 1.0050 .1500 % .4029 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.6530 .2468 % .6627 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0274 .0041 % .0110 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0263 .0039 % .0105 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, MULTI- FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 2141 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 540 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90918 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 669.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:32 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 7 X 5 MM, RED, DARK RED -B-ADENOMA,-NEOPLASM PRESENT AND TAN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2142 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 541 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90918 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 669.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:32 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2143 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 542 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90919 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/30/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/30/99 10:00 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; PAWS-FRONT; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL, FOCAL >SKIN & PELAGE; SORE/SCAB; EARS; KIDNEY (KD) : KIDNEY (KD) : VERIFIED AT NECROPSY: NOT OBSERVED -LARGE PELVIS(ES); LEFT -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -DILATATION, PELVIC,-PRESENT LUNG (LU) : LUNG (LU) : -MOTTLED; ALL LOBES: RED AND DARK RED -FIBROSIS, PLEURAL/SUBPLEURAL,- -ADHESION(S); ALL RIGHT LOBES TO EACH MODERATE, MULTI-FOCAL OTHER: MULTIPLE GREY FIBROUS -MINERALIZATION, VASCULAR,-MINIMAL, ADHESIONS; COLLECTED INTACT MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC URINARY BLADDER (UB) : URINARY BLADDER (UB) : -SEMIFLUID MATERIAL; DARK RED GRANULAR >UNREMARKABLE SEMIFLUID -RED FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM 2144 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 543 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90919 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/30/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/30/99 10:00 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : PROSTATE (PR) : -DIFFUSELY RED; DARK RED -EDEMA,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: GREY AND CLEAR -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL -LARGE; BILATERAL: EACH IS 30 X 15 X 5 MM -DIFFUSELY RED; BILATERAL: DARK RED CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; LEFT CARPAL JOINT: UP TO 10 MM -EXOSTOSES,-SEVERE, MULTI-FOCAL IN DIAMETER; COLLECTED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL GENERAL COMMENT (GC) : -URINARY BLADDER-LARGE LUMEN; 30 X 20 X 15 MM GENERAL INFORMATION (XX) : >NOTE:>SWOLLEN (RIGHT FRONT PAW) AS NOTED IN CLINICAL OBSERVATIONS WAS NOT OBSERVED AT TIME OF NECROPSY -------------------------------------------- 2145 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 544 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90919 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/30/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/30/99 10:00 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2146 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 545 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90920 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/22/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 643.5 GRAMS DATE AND TIME OF NECROPSY: 01/22/00 9:36 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; SCROTUM; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; NONFORMED FECES; VERIFIED -DILATATION, VENTRICULAR,-PRESENT AT NECROPSY: NOT APPLICABLE LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >APPEARANCE; HUNCHED; VERIFIED AT -VACUOLATION, HEPATOCELLULAR,-MODERATE, NECROPSY: NOT APPLICABLE MULTI-FOCAL >EYES; SQUINTED; PERI-ORBITAL; VERIFIED KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >DISCHARGE; NASAL; RED IN COLOR; FOCAL VERIFIED AT NECROPSY: OBSERVED -PYELONEPHRITIS,-SLIGHT, FOCAL >RESPIRATION; LABORED; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: NOT APPLICABLE -ANGIECTASIS,-SLIGHT, FOCAL -DEGENERATION, CYSTIC, CORTICAL,- SLIGHT, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL -HYPERTROPHY, CORTICAL CELL,-SLIGHT, FOCAL -THROMBUS,-SLIGHT, FOCAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, MULTI-FOCAL 2147 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 546 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90920 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/22/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 643.5 GRAMS DATE AND TIME OF NECROPSY: 01/22/00 9:36 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 5 X 5 MM, RED, GRAY AND -HEMORRHAGE,-MODERATE, MULTI-FOCAL FIRM -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL -BLOOD FILM TAKEN -DISCHARGE-NASAL; DRY RED MATERIAL -------------------------------------------- 2148 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 547 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90920 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/22/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 643.5 GRAMS DATE AND TIME OF NECROPSY: 01/22/00 9:36 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2149 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 548 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90921 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 520.7 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:24 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0248 .0048 % .0111 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0270 .0052 % .0121 WEIGHT TAKEN BRAIN (BR) 2.2400 .4302 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.5569 .4911 % 1.1415 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.6329 .5056 % 1.1754 WEIGHT TAKEN LIVER (LI) 29.9661 5.7550 % 13.3777 WEIGHT TAKEN LUNG (LU) 1.6740 .3215 % .7473 WEIGHT TAKEN SPLEEN (SP) .6825 .1311 % .3047 WEIGHT TAKEN LEFT TESTIS (TE0) 1.8368 .3528 % .8200 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.8132 .3482 % .8095 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0142 .0027 % .0063 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0149 .0029 % .0067 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, DIFFUSE -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL SKIN (SK) : SKIN (SK) : -RED FOCUS(I)/AREA(S); OVERLYING RIGHT >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CARPAL JOINT: SINGLE DARK RED AREA, 5 MM IN DIAMETER (CAP 21) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN 2150 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 549 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90921 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 520.7 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:24 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2151 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 550 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90922 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/12/99 STUDY DAY OF DEATH: 442 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 315.7 GRAMS DATE AND TIME OF NECROPSY: 04/12/99 15:32 PROSECTOR: NANCY DIEDRICH RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; YELLOW HAIR COAT; -DIFFUSELY DARK; ALL LOBES: DARK BROWN -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- VERIFIED AT NECROPSY: OBSERVED FOCAL >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >BEHAVIOR; HYPOACTIVE; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL -PYELONEPHRITIS,-SLIGHT, FOCAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT, MULTI- FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 MM IN DIAMETER, RED, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL DARK RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, URETHRA: GREEN AND FIRM MULTI-FOCAL 2152 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 551 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90922 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/12/99 STUDY DAY OF DEATH: 442 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 315.7 GRAMS DATE AND TIME OF NECROPSY: 04/12/99 15:32 PROSECTOR: NANCY DIEDRICH RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 30 X 15 X 5 -SECRETION, INCREASED,-PRESENT MM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -PIGMENT,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOWISH- BROWN MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -------------------------------------------- 2153 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 552 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90922 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/12/99 STUDY DAY OF DEATH: 442 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 315.7 GRAMS DATE AND TIME OF NECROPSY: 04/12/99 15:32 PROSECTOR: NANCY DIEDRICH RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2154 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 553 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90923 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/18/99 STUDY DAY OF DEATH: 601 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 465.7 GRAMS DATE AND TIME OF NECROPSY: 09/18/99 12:56 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >DISCHARGE; NASAL; RED IN COLOR; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; RED HAIR COAT; HEAD- -DILATATION, VENTRICULAR,-PRESENT CRANIAL; VERIFIED AT NECROPSY: NOT LIVER (LI) : OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >EXCRETION; FEW FECES; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE SLIGHT, MULTI-FOCAL >APPEARANCE; THIN; VERIFIED AT PANCREAS (PA) : NECROPSY: NOT OBSERVED -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 MM IN DIAMETER, RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL DARK RED, BLACK, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE -EROSION,-MINIMAL, FOCAL DARK BROWN AREA, 2 MM IN DIAMETER PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL 2155 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 554 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90923 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/18/99 STUDY DAY OF DEATH: 601 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 465.7 GRAMS DATE AND TIME OF NECROPSY: 09/18/99 12:56 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 13 X 5 X 2 >UNREMARKABLE MM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: LEFT IS 15 X 8 X 3 -DILATATION, SINUSOIDAL,-MODERATELY MM, RIGHT IS 13 X 5 X 3 MM; BOTH SEVERE, MULTI-FOCAL APPEAR GELATINOUS TAIL (TI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; BILATERAL: RED -STAINS - SKIN/HAIRCOAT; VENTRAL PERIANAL, PERINEUM, AND ABDOMINAL REGIONS: DARK RED -TAIL - BENT/MISSING; 46 MM FROM TIP, BENT: CORE OF TAIL IS EXPOSED; SURROUNDING TISSUE IS DARK RED; COLLECTED -------------------------------------------- 2156 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 555 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90923 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/18/99 STUDY DAY OF DEATH: 601 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 465.7 GRAMS DATE AND TIME OF NECROPSY: 09/18/99 12:56 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2157 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 556 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90924 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 614.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:21 PROSECTOR: JODI THIEREN RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0402 .0065 % .0166 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0332 .0054 % .0137 WEIGHT TAKEN BRAIN (BR) 2.4172 .3932 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.8755 .4678 % 1.1896 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.8181 .4585 % 1.1659 WEIGHT TAKEN LIVER (LI) 33.4640 5.4440 % 13.8441 WEIGHT TAKEN LUNG (LU) 2.1269 .3460 % .8799 WEIGHT TAKEN SPLEEN (SP) .8329 .1355 % .3446 WEIGHT TAKEN LEFT TESTIS (TE0) 1.8639 .3032 % .7711 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.8872 .3070 % .7807 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0199 .0032 % .0082 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0288 .0047 % .0119 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATELY SEVERE, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL LOWER INCISORS -BONE MARROW SMEAR TAKEN 2158 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 557 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90924 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 614.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:21 PROSECTOR: JODI THIEREN RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2159 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 558 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90925 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/02/99 STUDY DAY OF DEATH: 646 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 624.5 GRAMS DATE AND TIME OF NECROPSY: 11/02/99 14:45 PROSECTOR: JOHN S. HALFORD RECORDER: CHANESTA ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT -GRANULAR MATERIAL; RIGHT, WITHIN -NEPHROPATHY, CHRONIC PROGRESSIVE,- PELVIS: MULTIPLE TAN AND FIRM SLIGHT, MULTI-FOCAL GRANULES, PINPOINT AND UP TO 1 MM IN DIAMETER ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT, FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 4 MM: DARK RED, -HEMORRHAGE,-MINIMAL, MULTI-FOCAL GRAY, LOBULATED, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT 2160 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 559 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90925 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/02/99 STUDY DAY OF DEATH: 646 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 624.5 GRAMS DATE AND TIME OF NECROPSY: 11/02/99 14:45 PROSECTOR: JOHN S. HALFORD RECORDER: CHANESTA ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA, TISSUE -EDEMA,-MINIMAL, FOCAL SURROUNDING PYLORIC VALVE: MULTIPLE -EROSION,-MINIMAL, FOCAL DARK BROWN FOCI, UP TO 1 MM IN DIAMETER URINARY BLADDER (UB) : URINARY BLADDER (UB) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE DARK BROWN MUCOID SEMIFLUID MATERIAL PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, TISSUE SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,- URETHRA: GREEN AND FIRM MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -THICKENED; ALL REGIONS DIFFUSE: TAN >UNREMARKABLE AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: MOIST AND DRY YELLOW MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -DISCHARGE-NASAL; DRY RED MATERIAL -ANIMAL OBESE -------------------------------------------- 2161 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 560 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90925 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/02/99 STUDY DAY OF DEATH: 646 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 624.5 GRAMS DATE AND TIME OF NECROPSY: 11/02/99 14:45 PROSECTOR: JOHN S. HALFORD RECORDER: CHANESTA ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2162 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 561 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90926 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 432.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:59 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -MOTTLED; ALL LOBES, DIFFUSE: BROWN, -DEGENERATION, CYSTIC,-MODERATE, MULTI- >SKIN & PELAGE; YELLOW HAIR COAT; YELLOW AND DARK RED FOCAL VERIFIED AT NECROPSY: NOT OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >EYES; RED DISCHARGE; EYES; VERIFIED AT EOSINOPHILIC,-MODERATE, FOCAL NECROPSY: OBSERVED -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 22 X 12 X 8 -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE MM; BOTH APPEAR SOFT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 9 X 7 MM, TAN, RED AND -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SOFT -B-ADENOMA,-NEOPLASM PRESENT 2163 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 562 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90926 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 432.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:59 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY DARK RED MATERIAL -BONE MARROW SMEAR TAKEN -------------------------------------------- 2164 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 563 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90926 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 432.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:59 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2165 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 564 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90927 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 762.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:33 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 95; 70 X 65MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, MULTI- FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -MOTTLED; DARK RED AND TAN -HEMORRHAGE,-MODERATE, MULTI-FOCAL 2166 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 565 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90927 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 762.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:33 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -HYPERPLASIA, TYPE II PNEUMOCYTE,- SLIGHT, FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 5 MM IN DIAMETER, RED, DARK -HEMORRHAGE,-MODERATE, FOCAL RED, GRAY AND SOFT -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -B-LIPOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); LEFT AXILLARY REGION: -GALACTOCELE,-SLIGHT, FOCAL SINGLE MASS, 70 X 50 X 40 MM, TAN, >NOTE:>MAMMARY MASS DESCRIBED GROSSLY SOFT AND ADIPOSE TISSUE-LIKE; SAME ON WAS A SUBCUTANEOUS LIPOMA CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2167 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 566 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90927 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 762.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:33 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2168 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 567 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90928 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 491.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:21 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT PANCREAS (PA) : NECROPSY: OBSERVED -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL >APPEARANCE; THIN; VERIFIED AT LUNG (LU) : NECROPSY: NOT OBSERVED -MINERALIZATION,-MINIMAL, FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, MULTI- FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2169 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 568 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90928 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 491.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:21 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), LIVER, OIL RED O (LI1), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2170 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 569 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90929 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/17/99 13:18 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 20 X 10 X 8 >UNREMARKABLE MM LUNG (LU) : LUNG (LU) : -DIFFUSELY RED; ALL LOBES: DARK RED -HEMORRHAGE,-SEVERE, MULTI-FOCAL -MASS(ES); ALL LOBES: MULTIPLE MASSES, -INFLAMMATION, ACUTE,-SLIGHT, FOCAL UP TO 25 X 20 X 15 MM, DARK RED, TAN, -INFILTRATE, MACROPHAGE, PIGMENTED,- AND SOFT; ON SECTION EXUDED DARK RED MODERATE, MULTI-FOCAL FLUID AND DARK RED AND TAN SEMISOLID -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO MATERIAL; COLLECTED INTACT; REGIONAL NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN (SEE TRACHEOBRONCHIAL) PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC CAUSE OF DEATH (CD) : -MALIGNANT FIBROUS HISTIOSARCOMA,- PRESENT HEMATO NEOPLASIA (HN) : -M-MALIGNANT FIBROUS HISTIOCYTOMA,- NEOPLASM PRESENT 2171 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 570 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90929 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/17/99 13:18 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADIPOSE TISSUE (AT) : ADIPOSE TISSUE (AT) : -THICKENED; ALONG VENTRAL MID LINE: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO RED, TAN, AND FRIABLE (CAP 22) NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN, TRACHEOBRON (TB) : LN, TRACHEOBRON (TB) : -DIFFUSELY RED; DARK RED (CAP 21) -HEMORRHAGE,-SLIGHT, MULTI-FOCAL GENERAL COMMENT (GC) : -DISCHARGE-NASAL; DARK RED AND MOIST -MALOCCLUSION; BILATERAL UPPER INCISORS -FLUID-THORACIC CAVITY; DARK RED SEMIFLUID -------------------------------------------- 2172 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 571 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90929 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/17/99 13:18 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) ------------------------------------------------------------------------------------------------------------------------------------ 2173 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 572 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90930 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/11/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 587.0 GRAMS DATE AND TIME OF NECROPSY: 10/11/99 14:32 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -DIFFUSELY DARK; ALL LOBES: DARK BROWN -VACUOLATION, HEPATOCELLULAR,-MINIMAL, >APPEARANCE; MISSING; TEETH; VERIFIED MULTI-FOCAL AT NECROPSY: OBSERVED KIDNEY (KD) : >SKIN & PELAGE; BROWN HAIR COAT; -HYPERPLASIA, TRANSITIONAL CELL,- VENTRAL-ABDOMINAL; VERIFIED AT MODERATE, MULTI-FOCAL NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL -PYELONEPHRITIS,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MODERATE, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : URINARY BLADDER (UB) : -THICKENED WALL; DIFFUSE -CALCULUS,-PRESENT -CALCULUS(I); WITHIN LUMEN: MULTIPLE -HYPERPLASIA, TRANSITIONAL CELL,- TAN HARD CALCULI, UP TO 8 X 2 X 2 MM MODERATE, DIFFUSE -DIFFUSELY RED; MUCOSA: DARK RED PROSTATE (PR) : PROSTATE (PR) : -LARGE; 28 X 25 X 18 MM -INFLAMMATION, CHRONIC-ACTIVE,- -GELATINOUS; DIFFUSE: TAN AND CLEAR MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : -EDEMA,-MINIMAL, MULTI-FOCAL 2174 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 573 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90930 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/11/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 587.0 GRAMS DATE AND TIME OF NECROPSY: 10/11/99 14:32 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SEMINAL VESICLES (SV) : -FIBROSIS,-SEVERE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL >UNREMARKABLE LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL, LUMBAR: LEFT IS 10 -DILATATION, SINUSOIDAL,-MODERATE, X 7 X 4 MM, RIGHT IS 15 X 6 X 5 MM MULTI-FOCAL (CAP 23) -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL LOWER INCISORS -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DARK YELLOW AND MOIST -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: BROKEN AT BASE -------------------------------------------- 2175 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 574 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90930 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/11/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 587.0 GRAMS DATE AND TIME OF NECROPSY: 10/11/99 14:32 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2176 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 575 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90931 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 664.5 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 14:29 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >BEHAVIOR; ATAXIC; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -DEGENERATION, CYSTIC,-MODERATE, MULTI- >APPEARANCE; LIMITED USE; PAWS-FRONT; FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE -HYPERTROPHY, CENTRILOBULAR, >APPEARANCE; LIMITED USE; LIMBS-HIND; HEPATOCELLULAR,-MODERATE, MULTI-FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL SPLEEN (SP) : SPLEEN (SP) : -CYST(S); ALONG MARGIN AT CAUDAL END: -CYST, SEROSAL,-PRESENT MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER PANCREAS (PA) : PANCREAS (PA) : -MASS(ES); ADJACENT TO SPLEEN: SINGLE -B-ADENOMA, ISLET CELL,-NEOPLASM MASS, 7 X 5 X 5 MM, TAN, RED AND FIRM; PRESENT SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE PANCREATIC) MUSCLE, SKELETAL (SM) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT 2177 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 576 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90931 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 664.5 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 14:29 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-MINIMAL, MULTI- FOCAL CAUSE OF DEATH (CD) : -HEPATOCELLULAR ADENOMA,-PRESENT LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -DIFFUSELY RED; DARK RED (CAP 22) -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL OBESE -------------------------------------------- 2178 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 577 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90931 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 664.5 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 14:29 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2179 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 578 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90932 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 553.5 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:23 PROSECTOR: DONIA MEYER RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0318 .0057 % .0140 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0352 .0064 % .0155 WEIGHT TAKEN BRAIN (BR) 2.2771 .4114 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.3637 .4270 % 1.0380 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.3739 .4289 % 1.0425 WEIGHT TAKEN LIVER (LI) 23.8968 4.3174 % 10.4944 WEIGHT TAKEN LUNG (LU) 1.8995 .3432 % .8342 WEIGHT TAKEN SPLEEN (SP) .6502 .1175 % .2855 WEIGHT TAKEN LEFT TESTIS (TE0) 1.9335 .3493 % .8491 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.8941 .3422 % .8318 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0197 .0036 % .0087 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0205 .0037 % .0090 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT 2180 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 579 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90932 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 553.5 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:23 PROSECTOR: DONIA MEYER RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2181 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 580 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90932 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 553.5 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:23 PROSECTOR: DONIA MEYER RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2182 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 581 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90933 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 795.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:03 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -LARGE; ALL LOBES; MARGINS APPEAR -DEGENERATION, CYSTIC,-MINIMAL, MULTI- >MASS(ES); AXILLARY REGION-LEFT; WEEK ROUNDED FOCAL 88; 120 X 110MM; VERIFIED AT NECROPSY: -HYPERTROPHY, CENTRILOBULAR, OBSERVED HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -LARGE; UP TO 12 MM IN DIAMETER -DILATATION, SINUSOIDAL,-MODERATE, MULTI-FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYROID (TY) : -HEMORRHAGE,-MINIMAL, FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL SKIN (SK) : -B-LIPOMA,-NEOPLASM PRESENT 2183 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 582 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90933 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 795.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:03 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -DILATATION, SINUSOIDAL,-MODERATE, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); LEFT AXILLARY REGION: -PIGMENT,-MINIMAL, MULTI-FOCAL SINGLE MASS, 115 X 80 X 45 MM, RED, >NOTE:>MAMMARY MASS DESCRIBED GROSSLY TAN, VASCULAR AND SOFT; SAME ON CUT WAS A SUBCUTANEOUS LIPOMA SURFACE; PORTION COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2184 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 583 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90933 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 795.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:03 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2185 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 584 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90934 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 533.8 GRAMS DATE AND TIME OF NECROPSY: 01/14/00 15:23 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL; WEEK 102; BRAIN (BR) : BRAIN (BR) : 25 X 40MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE OBSERVED LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; BROWN HAIR COAT; -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -DEGENERATION, CYSTIC,-SLIGHT, FOCAL VENTRAL-ABDOMINAL; VERIFIED AT MULTIPLE TAN AREAS, UP TO 3 X 2 MM; -VACUOLATION, HEPATOCELLULAR,-MODERATE, NECROPSY: OBSERVED COLLECTED ON ROUTINE SECTIONS AND CAP MULTI-FOCAL >EXCRETION; FEW FECES; VERIFIED AT 22 -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NECROPSY: NOT APPLICABLE NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT >APPEARANCE; HUNCHED; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: NOT APPLICABLE -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT >DISCHARGE; ORAL; RED IN COLOR; -ROUGH SURFACE, DIFFUSE; BILATERAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO VERIFIED AT NECROPSY: OBSERVED -DIFFUSELY LIGHT; BILATERAL: TAN NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT >SKIN & PELAGE; RED HAIR COAT; HEAD- PANCREAS (PA) : ENTIRE; VERIFIED AT NECROPSY: NOT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO OBSERVED NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT >APPEARANCE; THIN; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: OBSERVED -VACUOLATION,-MODERATELY SEVERE, MULTI- >BEHAVIOR; HYPOACTIVE; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYMUS (TH) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LUNG (LU) : -HEMORRHAGE,-SLIGHT, FOCAL 2186 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 585 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90934 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 533.8 GRAMS DATE AND TIME OF NECROPSY: 01/14/00 15:23 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL MUSCLE, SKELETAL (SM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYROID (TY) : -CYST, FOLLICULAR,-MODERATELY SEVERE, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 5 X 4 MM, DARK RED, -HEMORRHAGE,-MINIMAL, MULTI-FOCAL TAN, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT SKIN (SK) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT URINARY BLADDER (UB) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PROSTATE (PR) : -NECROSIS,-SLIGHT, MULTI-FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2187 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 586 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90934 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 533.8 GRAMS DATE AND TIME OF NECROPSY: 01/14/00 15:23 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -MASS(ES); INVOLVING ENTIRE GLAND AND ADHERED TO SEMINAL VESICLES, URINARY BLADDER, AND DORSAL WALL OF ABDOMINAL CAVITY: SINGLE MASS, 55 X 40 X 40 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NOT IDENTIFIED SEMINAL VESICLES (SV) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -HISTIOCYTIC SARCOMA,-PRESENT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); VENTRAL ABDOMINAL REGION: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO SINGLE MASS, 70 X 40 X 30 MM, DARK NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -DISCHARGE-ORAL; DARK RED AND MOIST -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL REGION: BROWN AND MOIST -ANIMAL THIN -------------------------------------------- 2188 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 587 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90934 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 533.8 GRAMS DATE AND TIME OF NECROPSY: 01/14/00 15:23 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), EPIDIDYMIDES (EP) ------------------------------------------------------------------------------------------------------------------------------------ 2189 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 588 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90935 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 369.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:30 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 12.8689 3.4790 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2190 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 589 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90935 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 369.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:30 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2191 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 590 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90936 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/31/99 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 594.5 GRAMS DATE AND TIME OF NECROPSY: 12/31/99 13:44 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; -DILATATION, VENTRICULAR,-PRESENT VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >EXCRETION; NO FECES; VERIFIED AT -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC -HEMATOPOIESIS, EXTRAMEDULLARY,- NECROPSY: NOT APPLICABLE SURFACE OF LEFT LATERAL LOBE: MULTIPLE MINIMAL, MULTI-FOCAL >SKIN & PELAGE; ROUGH HAIR COAT; TAN AREAS, UP TO 3 MM IN DIAMETER; -B-CHOLANGIOMA,-NEOPLASM PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE COLLECTED ON ROUTINE SECTION >EXCRETION; DISCOLORED URINE; RED IN -CYST(S); ALONG MARGIN OF RIGHT MEDIAN COLOR; VERIFIED AT NECROPSY: OBSERVED LOBE: MULTIPLE CLEAR FLUID FILLED >BEHAVIOR; HYPOACTIVE; VERIFIED AT CYSTS, UP TO 8 X 6 X 5 MM; COLLECTED NECROPSY: NOT APPLICABLE ON ROUTINE SECTION >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT OBSERVED -CAST, PROTEINACEOUS,-SLIGHT, MULTI- >SKIN & PELAGE; PALE; ENTIRE BODY; FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE -HYPERPLASIA, TRANSITIONAL CELL,- SLIGHT, MULTI-FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL, MULTI-FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT 2192 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 591 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90936 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/31/99 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 594.5 GRAMS DATE AND TIME OF NECROPSY: 12/31/99 13:44 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, RED, DARK -HEMORRHAGE,-SLIGHT, MULTI-FOCAL RED AND SOFT -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : URINARY BLADDER (UB) : -THICKENED WALL; DIFFUSE -HYPERPLASIA, TRANSITIONAL CELL,- -CALCULUS(I); WITHIN LUMEN: MULTIPLE MODERATELY SEVERE, DIFFUSE TAN AND HARD CALCULI, UP TO 14 MM IN -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- DIAMETER FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: EACH IS UP TO 9 X 7 -DILATATION, SINUSOIDAL,-MODERATE, X 5 MM (CAP 22) MULTI-FOCAL LN, RENAL (RL) : -DILATATION, SINUSOIDAL,-SEVERE, FOCAL 2193 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 592 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90936 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/31/99 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 594.5 GRAMS DATE AND TIME OF NECROPSY: 12/31/99 13:44 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, RENAL (RL) : LN, RENAL (RL) : -CYST(S); INVOLVING ENTIRE RIGHT: -FIBROSIS,-MODERATE, FOCAL SINGLE CLEAR FLUID FILLED CYST, 22 MM IN DIAMETER (LEFT NOT IDENTIFIED) (CAP 23) FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL -EROSION,-MODERATE, MULTI-FOCAL HINDFEET: SINGLE DARK RED, TAN AND -FIBROSIS,-MODERATE, MULTI-FOCAL MOIST ABRADED AREA ON EACH, EACH IS 7 -INFLAMMATION, ACUTE,-MODERATE, MULTI- MM IN DIAMETER; BOTH COLLECTED FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; RED AND MOIST -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR: BROKEN OFF AT BASE -URINARY BLADDER-LARGE LUMEN; 32 X 20 X 20 MM -------------------------------------------- 2194 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 593 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90936 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/31/99 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 594.5 GRAMS DATE AND TIME OF NECROPSY: 12/31/99 13:44 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2195 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 594 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90937 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 398.5 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:43 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; HUNCHED; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >EXCRETION; NO FECES; VERIFIED AT -DEGENERATION, CYSTIC,-MODERATELY NECROPSY: NOT APPLICABLE SEVERE, FOCAL >SKIN & PELAGE; BROWN HAIR COAT; KIDNEY (KD) : KIDNEY (KD) : PERINEAL AREA; VERIFIED AT NECROPSY: -LARGE PELVIS(ES); BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- OBSERVED -ROUGH SURFACE, DIFFUSE; BILATERAL SEVERE, DIFFUSE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -VACUOLATION,-SLIGHT, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LN, MESENTERIC (MS) : -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, FOCAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 15 X 10 X 6 -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE MM; BOTH ARE SOFT -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL THYMUS (TH) : -CYST,-PRESENT 2196 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 595 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90937 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 398.5 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:43 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PARATHYROID (PT) : -HYPERPLASIA,-MODERATE, DIFFUSE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 10 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; RIGHT: 15 X 9 X 3 MM (LEFT IS >UNREMARKABLE 21 X 15 X 5 MM) CAUSE OF DEATH (CD) : -CHRONIC PROGRESSIVE NEPHROPATHY,- PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BROWN AND MOIST -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -MALOCCLUSION; BILATERAL: UPPER INCISORS -------------------------------------------- 2197 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 596 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90937 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 398.5 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:43 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2198 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 597 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90938 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/21/99 STUDY DAY OF DEATH: 481 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 488.7 GRAMS DATE AND TIME OF NECROPSY: 05/21/99 14:48 PROSECTOR: NANCY DIEDRICH RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HUNCHED; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED -DILATATION, VENTRICULAR,-PRESENT AT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; BROWN SKIN; VENTRAL- -LARGE; ALL LOBES: THICKENED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- ABDOMINAL; VERIFIED AT NECROPSY: -DIFFUSELY DARK; ALL LOBES: DARK BROWN FOCAL OBSERVED -VACUOLATION, HEPATOCELLULAR,-SLIGHT, >SKIN & PELAGE; BROWN HAIR COAT; HEAD- MULTI-FOCAL MAXILLARY-LEFT; VERIFIED AT NECROPSY: KIDNEY (KD) : KIDNEY (KD) : OBSERVED -LARGE PELVIS(ES); RIGHT -DILATATION, PELVIC,-PRESENT >SKIN & PELAGE; BROWN HAIR COAT; HEAD- -SEMIFLUID MATERIAL-PELVIS; RIGHT: -MINERALIZATION, PELVIC,-MODERATE, MAXILLARY-RIGHT; VERIFIED AT NECROPSY: GREENISH BROWN SEMIFLUID MULTI-FOCAL OBSERVED -MINERALIZATION, TUBULAR,-MINIMAL, >BEHAVIOR; HYPOACTIVE; VERIFIED AT MULTI-FOCAL NECROPSY: NOT APPLICABLE HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- BROWN AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EDEMA,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 2199 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 598 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90938 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/21/99 STUDY DAY OF DEATH: 481 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 488.7 GRAMS DATE AND TIME OF NECROPSY: 05/21/99 14:48 PROSECTOR: NANCY DIEDRICH RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL -ULCERATION,-MODERATE, FOCAL STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -EDEMA,-MODERATE, MULTI-FOCAL RED AND TAN ERODED AREAS, UP TO 4 X 2 -HEMORRHAGE,-SLIGHT, MULTI-FOCAL MM -INFLAMMATION, ACUTE,-MINIMAL, MULTI- -THICKENED WALL; MUCOSA: DIFFUSE FOCAL -ULCERATION,-MODERATE, FOCAL PROSTATE (PR) : PROSTATE (PR) : -LARGE; 25 X 18 X 8 MM -INFLAMMATION, CHRONIC-ACTIVE,- -NODULAR; PROXIMAL, SURROUNDING MODERATELY SEVERE, MULTI-FOCAL URETHRA: DARK GREEN AND FIRM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-ORAL; DARK BROWN AND DRY -STAINS-PERINEUM/PERIANAL; DARK BROWN AND MOIST -BLOOD FILM TAKEN -DISCHARGE-NASAL; DARK BROWN AND DRY -------------------------------------------- 2200 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 599 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90938 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/21/99 STUDY DAY OF DEATH: 481 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 488.7 GRAMS DATE AND TIME OF NECROPSY: 05/21/99 14:48 PROSECTOR: NANCY DIEDRICH RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2201 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 600 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90939 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 316.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:22 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 12.4132 3.9245 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2202 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 601 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90939 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 316.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:22 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2203 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 602 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90940 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/12/99 STUDY DAY OF DEATH: 442 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 486.3 GRAMS DATE AND TIME OF NECROPSY: 04/12/99 15:47 PROSECTOR: NANCY DIEDRICH RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; VENTRAL- BRAIN (BR) : BRAIN (BR) : ABDOMINAL; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; BROWN HAIR COAT; LIVER (LI) : LIVER (LI) : PERINEAL AREA; VERIFIED AT NECROPSY: -DIFFUSELY LIGHT; ALL LOBES: TAN -VACUOLATION, HEPATOCELLULAR,- OBSERVED MODERATELY SEVERE, MULTI-FOCAL >EYES; RED DISCHARGE; EYES; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: OBSERVED -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- >SKIN & PELAGE; RED HAIR COAT; HEAD- MINIMAL, MULTI-FOCAL ENTIRE; VERIFIED AT NECROPSY: OBSERVED PANCREAS (PA) : >APPEARANCE; THIN; VERIFIED AT -INFILTRATION, FAT, INTERSTITIAL,- NECROPSY: OBSERVED MINIMAL, MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -PIGMENT,-MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE PITUITARY (PI) : PITUITARY (PI) : >APPEARANCE; HUNCHED; VERIFIED AT -MASS(ES); 10 MM IN DIAMETER, RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL NECROPSY: NOT APPLICABLE DARK RED, GRAY, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT >EXCRETION; NO FECES; VERIFIED AT STOMACH, GL (ST) : STOMACH, GL (ST) : NECROPSY: NOT APPLICABLE -DARK FOCUS(I)/AREA(S); MUCOSA: -INFLAMMATION, ACUTE,-MINIMAL, FOCAL >APPEARANCE; HEAD TILT; LEFT; VERIFIED MULTIPLE DARK BROWN PINPOINT FOCI AT NECROPSY: NOT APPLICABLE STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : >RESPIRATION; LABORED; VERIFIED AT -THICKENED WALL; MUCOSA, DIFFUSE >UNREMARKABLE NECROPSY: NOT APPLICABLE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL 2204 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 603 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90940 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/12/99 STUDY DAY OF DEATH: 442 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 486.3 GRAMS DATE AND TIME OF NECROPSY: 04/12/99 15:47 PROSECTOR: NANCY DIEDRICH RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BROWN AND DRY MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -DISCHARGE-NASAL; DARK RED AND DRY -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE AND HEAD: DARK RED AND DRY MATERIAL -ANIMAL THIN -------------------------------------------- 2205 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 604 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90940 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/12/99 STUDY DAY OF DEATH: 442 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 486.3 GRAMS DATE AND TIME OF NECROPSY: 04/12/99 15:47 PROSECTOR: NANCY DIEDRICH RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2206 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 605 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90941 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 449.0 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 9:56 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: OBSERVED LIVER (LI) : >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -DEGENERATION, CYSTIC,-SLIGHT, MULTI- AT NECROPSY: NOT APPLICABLE FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -VACUOLATION, HEPATOCELLULAR,-MODERATE, NECROPSY: NOT APPLICABLE MULTI-FOCAL >EYES; RED DISCHARGE; EYES; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >APPEARANCE; HUNCHED; VERIFIED AT SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE -PYELONEPHRITIS,-MODERATE, MULTI-FOCAL >SKIN & PELAGE; COLD TO TOUCH; ENTIRE ADRENAL, CORTEX (AC) : BODY; VERIFIED AT NECROPSY: NOT -ANGIECTASIS,-SLIGHT, FOCAL APPLICABLE -HEMORRHAGE,-SLIGHT, FOCAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 11 X 10 X 10 -HEMORRHAGE,-MODERATELY SEVERE, MULTI- MM, RED, DARK RED, TAN, GRAY, AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL 2207 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 606 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90941 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 449.0 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 9:56 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -EDEMA,-SLIGHT, FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE -EDEMA,-MODERATELY SEVERE, MULTI-FOCAL BROWN ULCERATED AREA, 10 X 5 MM -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL -ULCERATION,-SEVERE, FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; RIGHT: 35 X 20 X 7 MM (LEFT IS >UNREMARKABLE 25 X 14 X 5 MM) CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY, CRUSTED MATERIAL -ANIMAL THIN -------------------------------------------- 2208 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 607 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90941 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 449.0 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 9:56 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2209 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 608 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90942 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 329.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:22 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 11.6629 3.5417 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2210 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 609 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90942 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 329.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:22 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2211 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 610 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90943 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/26/99 STUDY DAY OF DEATH: 639 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/26/99 13:20 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT EYE (EY) : NECROPSY: OBSERVED -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO >APPEARANCE; THIN; VERIFIED AT NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT NECROPSY: OBSERVED BRAIN (BR) : BRAIN (BR) : >EXCRETION; NO FECES; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; YELLOW HAIR COAT; -RED FOCUS(I)/AREA(S); ALL LOBES: -VACUOLATION, HEPATOCELLULAR,-MODERATE, VERIFIED AT NECROPSY: OBSERVED MULTIPLE DARK RED AREAS, UP TO 4 X 3 MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT MM; COLLECTED ON ROUTINE SECTIONS NECROPSY: NOT APPLICABLE KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT -CAST, PROTEINACEOUS,-MINIMAL, MULTI- -MASS(ES); ATTACHED TO RIGHT: SINGLE FOCAL MASS, 35 X 29 X 16 MM, RED, GRAY, TAN, -DILATATION, PELVIC,-PRESENT LOBULATED AND SOFT; SAME ON CUT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO SURFACE; COLLECTED INTACT; REGIONAL LN NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT (RENAL) NORMAL (CAP 24) PANCREAS (PA) : PANCREAS (PA) : -MASS(ES); ATTACHED TO DUODENUM: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO SINGLE MASS, 20 X 18 X 6 MM, RED, NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT GRAY, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (PANCREATIC) NOT IDENTIFIED ADRENAL, CORTEX (AC) : -ONE EXAMINED,-PRESENT 2212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 611 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90943 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/26/99 STUDY DAY OF DEATH: 639 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/26/99 13:20 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -MISSING/NOT IDENTIFIED; RIGHT: DUE TO -VACUOLATION,-MINIMAL, MULTI-FOCAL MASS, ATTACHED TO RIGHT KIDNEY; ADRENAL NOT IDENTIFIED ADRENAL, MEDULLA (MA) : -ONE EXAMINED,-PRESENT LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY RED; DARK RED -HEMORRHAGE,-MODERATE, MULTI-FOCAL TESTIS (TE) : TESTIS (TE) : -DIFFUSELY RED; RIGHT -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL -NECROSIS,-SEVERE, DIFFUSE THYMUS (TH) : THYMUS (TH) : -MASS(ES); INVOLVING ENTIRE ORGAN: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO SINGLE MASS, 16 X 13 X 11 MM, RED, NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT DARK RED, GRAY, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE MEDIASTINAL) LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); LEFT LOBE: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO MULTIPLE TAN FOCI, UP TO 1 MM IN NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT DIAMETER -ADHESION(S); ALL LOBES TO PLEURA AND DIAPHRAGM: MULTIPLE GRAY, TAN, AND FIBROUS ADHESIONS; COLLECTED INTACT 2213 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 612 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90943 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/26/99 STUDY DAY OF DEATH: 639 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/26/99 13:20 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -MASS(ES); INVOLVING ALL RIGHT LOBES: SINGLE MASS, 41 X 23 X 18 MM, RED, DARK RED, GRAY, TAN, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE TRACHEOBRONCHIAL) HEART (HT) : HEART (HT) : -LARGE; RIGHT ATRIUM: 15 X 9 X 6 MM -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO (LEFT IS 5 X 4 X 3 MM) NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -MOTTLED; EPICARDIUM, DIFFUSE: DARK RED AND GRAY PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 6 X 4 MM, TAN AND SOFT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA, TISSUE -EROSION,-MODERATE, FOCAL SURROUNDING PYLORIC VALVE: MULTIPLE -HEMORRHAGE,-MINIMAL, FOCAL RED AREAS, UP TO 2 X 1 MM -INFLAMMATION, ACUTE,-MINIMAL, FOCAL -THICKENED WALL; MUCOSA STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE >UNREMARKABLE TAN AND RED ERODED AREAS, UP TO 3 X 2 MM SKIN (SK) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PROSTATE (PR) : PROSTATE (PR) : -SMALL; 9 X 6 X 3 MM >UNREMARKABLE 2214 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 613 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90943 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/26/99 STUDY DAY OF DEATH: 639 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/26/99 13:20 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 13 X 8 X 3 >UNREMARKABLE MM CAUSE OF DEATH (CD) : -MALIGNANT LYMPHOMA,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -LARGE; UP TO 8 X 5 X 4 MM (CAP 23) -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -DIFFUSELY RED; DARK RED -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT LN, RENAL (RL) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN, TRACHEOBRON (TB) : LN, TRACHEOBRON (TB) : -DIFFUSELY RED; DARK RED -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO -LARGE; UP TO 12 X 8 X 5 MM (CAP 22) NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -MALOCCLUSION; BILATERAL: UPPER INCISORS -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DRY, RED, DARK RED AND YELLOW MATERIAL -ANIMAL THIN -------------------------------------------- 2215 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 614 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90943 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/26/99 STUDY DAY OF DEATH: 639 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/26/99 13:20 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP) ------------------------------------------------------------------------------------------------------------------------------------ 2216 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 615 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90944 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 626.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:06 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); BOTH MEDIAN -DEGENERATION, CYSTIC,-SLIGHT, MULTI- LOBES: MULTIPLE TAN AREAS, UP TO 2 X 1 FOCAL MM; COLLECTED ON ROUTINE SECTION -FOCUS, ALTERED HEPATOCELLULAR, -DARK FOCUS(I)/AREA(S); VISCERAL EOSINOPHILIC,-MODERATELY SEVERE, FOCAL SURFACE OF LEFT LATERAL LOBE: SINGLE -HYPERTROPHY, CENTRILOBULAR, DARK BROWN AREA, 5 MM IN DIAMETER; HEPATOCELLULAR,-MODERATE, DIFFUSE COLLECTED ON ROUTINE SECTION -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -ADRENAL TISSUE, ECTOPIC,-SLIGHT, FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK RED PINPOINT FOCI CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2217 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 616 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90944 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 626.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:06 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2218 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 617 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90945 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/30/98 STUDY DAY OF DEATH: 309 STUDY WEEK OF DEATH: 45 TERMINAL BODY WEIGHT: 549.0 GRAMS DATE AND TIME OF NECROPSY: 11/30/98 10:14 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >DISCHARGE; NASAL; RED IN COLOR; THYMUS (TH) : THYMUS (TH) : VERIFIED AT NECROPSY: OBSERVED -DIFFUSELY RED; DARK RED -HEMORRHAGE,-SLIGHT, MULTI-FOCAL >SKIN & PELAGE; BROWN HAIR COAT; LUNG (LU) : VENTRAL-ABDOMINAL; VERIFIED AT -INFILTRATE, MACROPHAGE, ALVEOLAR,- NECROPSY: NOT OBSERVED SLIGHT, MULTI-FOCAL >RESPIRATION; AUDIBLE; VERIFIED AT STOMACH, GL (ST) : STOMACH, GL (ST) : NECROPSY: NOT APPLICABLE -THICKENED WALL; DIFFUSE >UNREMARKABLE CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-ORAL; DRY DARK RED MATERIAL -BLOOD FILM TAKEN -DISCHARGE-NASAL; DRY DARK RED MATERIAL -------------------------------------------- 2219 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 618 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90945 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/30/98 STUDY DAY OF DEATH: 309 STUDY WEEK OF DEATH: 45 TERMINAL BODY WEIGHT: 549.0 GRAMS DATE AND TIME OF NECROPSY: 11/30/98 10:14 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2220 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 619 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90946 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/19/99 11:48 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT OBSERVED LIVER (LI) : >HOUSING,FOOD,WATER & MAINT.; TEETH -CYST,-SLIGHT, MULTI-FOCAL CUT; VERIFIED AT NECROPSY: NOT KIDNEY (KD) : APPLICABLE -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >EYES; RED DISCHARGE; EYE-LEFT; FOCAL VERIFIED AT NECROPSY: OBSERVED SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MINIMAL, FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: RED, DRY MATERIAL 2221 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 620 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90946 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/19/99 11:48 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL UPPER AND LOWER INCISORS -GI TRACT-LARGE LUMEN; ENTIRE GI TRACT: DISTENDED WITH GAS -------------------------------------------- 2222 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 621 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90946 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/19/99 11:48 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2223 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 622 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90947 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 501.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:30 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; DORSAL- LIVER (LI) : LIVER (LI) : THORACIC-RIGHT; VERIFIED AT NECROPSY: -MASS(ES); RIGHT MEDIAN LOBE: SINGLE -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- OBSERVED MASS, 20 X 15 X 10 MM, TAN AND FIRM; FOCAL SAME ON CUT SURFACE; COLLECTED ON -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- ROUTINE SECTION; REGIONAL LN (HEPATIC) FOCAL NORMAL (CAP 22) -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL THYROID (TY) : THYROID (TY) : -LARGE; BILATERAL: EACH IS 8 X 6 X 4 -B-ADENOMA, C-CELL,-NEOPLASM PRESENT MM -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT 2224 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 623 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90947 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 501.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:30 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 4 X 4 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE >UNREMARKABLE -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 2 MM IN DIAMETER SKIN (SK) : SKIN (SK) : -MASS(ES); RIGHT DORSAL THORACIC -HEMORRHAGE,-SLIGHT, MULTI-FOCAL REGION: SINGLE MASS, 8 MM IN DIAMETER, -B-KERATOACANTHOMA,-NEOPLASM PRESENT TAN, FIRM AND FRIABLE; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 23) URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL -MINERALIZATION, LUMINAL,-MODERATE, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LN, OTHER (LN) : -DILATATION, SINUSOIDAL,-MODERATE, FOCAL 2225 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 624 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90947 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 501.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:30 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, OTHER (LN) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL >NOTE:>HEPATIC LYMPH NODE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2226 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 625 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90947 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 501.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:30 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2227 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 626 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90948 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 606.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:48 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -MOTTLED; ALL LOBES: RED, TAN, AND -VACUOLATION, HEPATOCELLULAR,-MODERATE, >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; BROWN MULTI-FOCAL VERIFIED AT NECROPSY: OBSERVED -MASS(ES); RIGHT LATERAL LOBE: SINGLE -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM MASS, 27 X 18 X 13 MM, RED, DARK RED, PRESENT TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (HEPATIC) NOT IDENTIFIED -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE RED AREAS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : PITUITARY (PI) : -RAISED AREA(S); LEFT POSTERIOR -CYST,-PRESENT ASPECT: SINGLE TAN AREA, 1 MM IN -HEMORRHAGE,-MINIMAL, MULTI-FOCAL DIAMETER -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE >UNREMARKABLE TAN ERODED AREAS, UP TO 2 X 2 MM RECTUM (RE) : RECTUM (RE) : >NOTE:>TRIMMING COMMENT: RECTUM NOT >TISSUE MISSING FOUND AT TRIMMING 2228 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 627 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90948 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 606.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:48 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, ACUTE,-MINIMAL, MULTI- FOCAL -MINERALIZATION, LUMINAL,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); BILATERAL HINDFEET: -EROSION,-MODERATELY SEVERE, MULTI- SINGLE DARK RED AND TAN AREA ON EACH, FOCAL BOTH ARE 10 MM IN DIAMETER; BOTH -EXOSTOSES,-MODERATELY SEVERE, MULTI- COLLECTED FOCAL -INFLAMMATION, CHRONIC ACTIVE,- MODERATE, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: RED -MALOCCLUSION; BILATERAL: UPPER INCISORS -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN OFF AT BASE -------------------------------------------- 2229 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 628 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90948 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 606.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:48 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2230 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 629 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90949 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/21/98 STUDY DAY OF DEATH: 86 STUDY WEEK OF DEATH: 13 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/21/98 14:57 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: NOT OBSERVED -HYPERTROPHY, CENTRILOBULAR, >APPEARANCE; THIN; VERIFIED AT HEPATOCELLULAR,-MODERATE, DIFFUSE NECROPSY: OBSERVED KIDNEY (KD) : >SKIN & PELAGE; BROWN HAIR COAT; -CAST, PROTEINACEOUS,-MINIMAL, FOCAL SCROTUM; VERIFIED AT NECROPSY: SPLEEN (SP) : SPLEEN (SP) : OBSERVED -SMALL; 28 X 7 X 3 MM >UNREMARKABLE >EXCRETION; NONFORMED FECES; VERIFIED THYMUS (TH) : AT NECROPSY: NOT APPLICABLE -HEMORRHAGE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; PERIANAL REGION: DRY BROWN STAINS -ANIMAL THIN -------------------------------------------- 2231 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 630 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90949 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/21/98 STUDY DAY OF DEATH: 86 STUDY WEEK OF DEATH: 13 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/21/98 14:57 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2232 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 631 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90950 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 700.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:16 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0261 .0037 % .0104 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0247 .0035 % .0099 WEIGHT TAKEN BRAIN (BR) 2.5063 .3577 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.6943 .3846 % 1.0750 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.7028 .3858 % 1.0784 WEIGHT TAKEN LIVER (LI) 37.5350 5.3576 % 14.9763 WEIGHT TAKEN LUNG (LU) 2.7288 .3895 % 1.0888 WEIGHT TAKEN SPLEEN (SP) 1.6852 .2405 % .6724 WEIGHT TAKEN LEFT TESTIS (TE0) 1.4975 .2137 % .5975 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.4889 .2125 % .5941 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0262 .0037 % .0105 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0272 .0039 % .0109 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATELY SEVERE, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL -STASIS, BILE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HYPERPLASIA,-MODERATE, FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: TWO -EROSION,-MINIMAL, MULTI-FOCAL BROWN AREAS, EACH IS 2 X 1 MM 2233 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 632 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90950 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 700.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:16 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ COLON (CO) : -PARASITISM,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2234 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 633 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90950 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 700.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:16 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2235 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 634 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90951 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 613.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:34 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0277 .0045 % .0118 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0538 .0088 % .0228 WEIGHT TAKEN BRAIN (BR) 2.3563 .3839 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.3550 .3837 % .9994 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.4057 .3920 % 1.0210 WEIGHT TAKEN LIVER (LI) 36.9152 6.0152 % 15.6666 WEIGHT TAKEN LUNG (LU) 2.0966 .3416 % .8898 WEIGHT TAKEN SPLEEN (SP) .9610 .1566 % .4078 WEIGHT TAKEN LEFT TESTIS (TE0) 1.9872 .3238 % .8434 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.9500 .3177 % .8276 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0254 .0041 % .0108 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0242 .0039 % .0103 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, >APPEARANCE; MISSING; TEETH; VERIFIED HEPATOCELLULAR,-MODERATELY SEVERE, AT NECROPSY: OBSERVED MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL ADRENAL, MEDULLA (MA) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT 2236 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 635 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90951 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 613.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:34 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR, MISSING; RIGHT UPPER INCISOR, BROKEN AT BASE -------------------------------------------- 2237 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 636 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90951 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 613.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:34 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2238 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 637 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90952 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/12/99 STUDY DAY OF DEATH: 411 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: 581.8 GRAMS DATE AND TIME OF NECROPSY: 03/12/99 7:41 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -DILATATION, VENTRICULAR,-PRESENT AT NECROPSY: NOT APPLICABLE LIVER (LI) : >RESPIRATION; AUDIBLE; VERIFIED AT -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED -VACUOLATION, HEPATOCELLULAR,-MINIMAL, AT NECROPSY: NOT APPLICABLE MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL 2239 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 638 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90952 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/12/99 STUDY DAY OF DEATH: 411 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: 581.8 GRAMS DATE AND TIME OF NECROPSY: 03/12/99 7:41 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL OBESE -------------------------------------------- 2240 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 639 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90952 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/12/99 STUDY DAY OF DEATH: 411 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: 581.8 GRAMS DATE AND TIME OF NECROPSY: 03/12/99 7:41 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2241 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 640 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90953 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 727.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:52 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -MOTTLED; ALL LOBES: DARK RED AND TAN -DEGENERATION, CYSTIC,-MINIMAL, MULTI- FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 16 X 14 X 4 -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE MM; BOTH ARE SOFT THYROID (TY) : THYROID (TY) : -LARGE; BILATERAL: LEFT IS 7 X 5 X 5 -MINERALIZATION,-MINIMAL, MULTI-FOCAL MM, RIGHT IS 7 X 3 X 3 MM -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : PARATHYROID (PT) : -LARGE; LEFT: 2 MM IN DIAMETER (RIGHT >SECTION EXAMINED; TISSUE NOT PRESENT IS LESS THAN 1 MM IN DIAMETER) PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); DIFFUSE: -HEMORRHAGE,-SLIGHT, MULTI-FOCAL MULTIPLE DARK BROWN PINPOINT FOCI -B-ADENOMA,-NEOPLASM PRESENT 2242 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 641 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90953 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 727.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:52 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -EDEMA,-MINIMAL, FOCAL -EROSION,-MINIMAL, FOCAL EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -DILATATION, SINUSOIDAL,-MODERATE, FOCAL -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, DIFFUSE MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); RIGHT CERVICAL REGION: -B-ADENOMA,-NEOPLASM PRESENT SINGLE MASS, 20 MM IN DIAMETER, RED, DARK RED, TAN, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2243 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 642 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90953 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 727.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:52 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2244 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 643 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90954 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/19/99 STUDY DAY OF DEATH: 479 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 430.4 GRAMS DATE AND TIME OF NECROPSY: 05/19/99 10:26 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; YELLOW HAIR COAT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; ROUGH HAIR COAT; -LIGHT FOCUS(I)/AREA(S); JUNCTION OF -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- VERIFIED AT NECROPSY: NOT APPLICABLE MEDIAN LOBES: MULTIPLE TAN AREAS, UP FOCAL >EXCRETION; NO FECES; VERIFIED AT TO 2 X 1 MM; COLLECTED ON ROUTINE -LIPIDOSIS, SUBCAPSULAR (TENSION NECROPSY: NOT APPLICABLE SECTION LIPIDOSIS),-MODERATELY SEVERE, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, FOCAL HEART (HT) : -MINERALIZATION,-MINIMAL, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 9 X 8 MM, RED, GREY, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL TAN, LOBULATED AND SOFT -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2245 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 644 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90954 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/19/99 STUDY DAY OF DEATH: 479 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 430.4 GRAMS DATE AND TIME OF NECROPSY: 05/19/99 10:26 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: DRY, YELLOW MATERIAL -BLOOD FILM TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2246 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 645 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90955 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 765.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 9:45 PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; TAIL-PROXIMAL; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -DEGENERATION, CYSTIC,-MODERATE, MULTI- MULTIPLE DARK RED AREAS, UP TO 4 X 3 FOCAL MM; COLLECTED ON ROUTINE SECTIONS -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATELY SEVERE, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); MID ASPECT: -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SINGLE DARK BROWN FOCUS, 1 MM IN -B-ADENOMA,-NEOPLASM PRESENT DIAMETER HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL 2247 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 646 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90955 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 765.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 9:45 PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT TAIL (TI) : TAIL (TI) : -LARGE; PROXIMAL ONE-HALF OF TAIL: UP -EXOSTOSES,-MODERATE, MULTI-FOCAL TO 19 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2248 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 647 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90955 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 765.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 9:45 PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2249 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 648 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90956 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 597.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:25 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT EYE (EY) : NECROPSY: OBSERVED -INFLAMMATION, ACUTE, CORNEAL,-MINIMAL, >EXCRETION; NO FECES; VERIFIED AT MULTI-FOCAL NECROPSY: NOT APPLICABLE BRAIN (BR) : BRAIN (BR) : >APPEARANCE; THIN; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; HUNCHED; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -MOTTLED; ALL LOBES: RED AND TAN -DEGENERATION, CYSTIC,-SLIGHT, MULTI- >SKIN & PELAGE; BROWN HAIR COAT; FOCAL VENTRAL-ABDOMINAL; VERIFIED AT -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- NECROPSY: OBSERVED FOCAL >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- AT NECROPSY: NOT APPLICABLE FOCAL -VACUOLATION, HEPATOCELLULAR,-SEVERE, MULTI-FOCAL KIDNEY (KD) : KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL >UNREMARKABLE -DIFFUSELY LIGHT; BILATERAL: TAN TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 16 X 8 X 7 -MINERALIZATION,-SLIGHT, FOCAL MM; BOTH APPEAR SOFT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 2250 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 649 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90956 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 597.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:25 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EDEMA,-MODERATELY SEVERE, FOCAL MULTIPLE DARK BROWN AREAS, UP TO 4 X 2 MM PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; EXTENDING INTO VENTRAL ABDOMINAL REGION: MOIST BROWN MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED MATERIAL -MALOCCLUSION; LEFT UPPER INCISOR -BONE MARROW SMEAR TAKEN 2251 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 650 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90956 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 597.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:25 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR: BROKEN OFF AT BASE RIGHT UPPER INCISOR: MISSING -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2252 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 651 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90957 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 549.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:35 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0384 .0070 % .0172 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0317 .0058 % .0142 WEIGHT TAKEN BRAIN (BR) 2.2324 .4062 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.2615 .4115 % 1.0130 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.1128 .3844 % .9464 WEIGHT TAKEN LIVER (LI) 30.0667 5.4707 % 13.4683 WEIGHT TAKEN LUNG (LU) 2.3390 .4256 % 1.0478 WEIGHT TAKEN SPLEEN (SP) .6621 .1205 % .2966 WEIGHT TAKEN LEFT TESTIS (TE0) 1.8336 .3336 % .8214 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.8700 .3402 % .8377 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0171 .0031 % .0077 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0196 .0036 % .0088 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, >APPEARANCE; MISSING; TEETH; VERIFIED HEPATOCELLULAR,-MODERATE, MULTI-FOCAL AT NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL LUNG (LU) : -HYPERPLASIA, MEDIAL, ARTERIAL/ARTERIOLAR,-SLIGHT, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2253 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 652 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90957 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 549.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:35 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY MATERIAL -MALOCCLUSION; RIGHT UPPER INCISOR -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; MISSING: LEFT UPPER INCISOR -------------------------------------------- 2254 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 653 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90957 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 549.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:35 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2255 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 654 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90958 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/12/99 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/12/99 7:41 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; -DILATATION, VENTRICULAR,-PRESENT VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : >HOUSING,FOOD,WATER & MAINT.; TEETH -DEGENERATION, CYSTIC,-SLIGHT, MULTI- CUT; VERIFIED AT NECROPSY: NOT FOCAL APPLICABLE -HYPERPLASIA, BILE DUCT,-SLIGHT, MULTI- >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; FOCAL VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : KIDNEY (KD) : -GRANULAR MATERIAL; RIGHT, WITHIN -MINERALIZATION, TUBULAR,-MINIMAL, PELVIS: MULTIPLE TAN AND FIRM MULTI-FOCAL GRANULES, UP TO 1 MM IN DIAMETER ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, DARK RED, TAN -HEMORRHAGE,-MINIMAL, MULTI-FOCAL AND SOFT -B-ADENOMA,-NEOPLASM PRESENT 2256 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 655 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90958 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/12/99 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/12/99 7:41 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ COLON (CO) : -PARASITISM,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); BILATERAL HINDFEET: -EROSION,-MODERATELY SEVERE, MULTI- SINGLE DRY, DARK RED CRUSTED AREA ON FOCAL EACH, RIGHT IS 12 X 12 MM, LEFT IS 10 -EXOSTOSES,-SEVERE, MULTI-FOCAL X 6 MM; TISSUE SURROUNDING LEFT -FIBROSIS,-MODERATE, MULTI-FOCAL APPEARS SWOLLEN; BOTH COLLECTED -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL GENERAL COMMENT (GC) : -MALOCCLUSION; UPPER LEFT INCISOR -TEETH MISSING/BROKEN/WORN; UPPER RIGHT INCISOR: MISSING -------------------------------------------- 2257 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 656 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90958 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/12/99 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/12/99 7:41 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2258 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 657 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90959 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 654.7 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 14:49 PROSECTOR: JAMES T. CALLEN RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HEAD TILT; LEFT; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EYES; RED DISCHARGE; EYE-RIGHT; -DILATATION, VENTRICULAR,-PRESENT VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >BEHAVIOR; ATAXIC; VERIFIED AT -RED FOCUS(I)/AREA(S); ALL LOBES: -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE MULTIPLE DARK RED AREAS, UP TO 3 X 2 FOCAL >EXCRETION; FEW FECES; VERIFIED AT MM; COLLECTED ON ROUTINE SECTIONS -VACUOLATION, HEPATOCELLULAR,-MINIMAL, NECROPSY: NOT APPLICABLE MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL SPLEEN (SP) : -B-HEMANGIOMA,-NEOPLASM PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 5 X 5 MM, RED, DARK -HEMORRHAGE,-MINIMAL, MULTI-FOCAL RED, TAN, LOBULATED AND FIRM -B-ADENOMA,-NEOPLASM PRESENT SALIV GL, MANDIB (SG) : SALIV GL, MANDIB (SG) : >NOTE:>TRIMMING COMMENT: NOT FOUND WITH >TISSUE MISSING WET TISSUE 2259 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 658 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90959 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 654.7 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 14:49 PROSECTOR: JAMES T. CALLEN RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY AND BROWN MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DRY AND RED MATERIAL -------------------------------------------- 2260 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 659 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90959 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 654.7 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 14:49 PROSECTOR: JAMES T. CALLEN RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2261 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 660 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90960 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 480.3 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:31 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0336 .0070 % .0163 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0328 .0068 % .0159 WEIGHT TAKEN BRAIN (BR) 2.0634 .4296 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.9514 .4063 % .9457 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.0451 .4258 % .9911 WEIGHT TAKEN LIVER (LI) 24.0869 5.0150 % 11.6734 WEIGHT TAKEN LUNG (LU) 1.4765 .3074 % .7156 WEIGHT TAKEN SPLEEN (SP) .6268 .1305 % .3038 WEIGHT TAKEN LEFT TESTIS (TE0) 1.6023 .3336 % .7765 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.6563 .3448 % .8027 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0145 .0030 % .0070 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0125 .0026 % .0061 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2262 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 661 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90960 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 480.3 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:31 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2263 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 662 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90961 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 309.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:06 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 10.9577 3.5359 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2264 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 663 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90961 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 309.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:06 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2265 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 664 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90962 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/01/99 STUDY DAY OF DEATH: 492 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 475.5 GRAMS DATE AND TIME OF NECROPSY: 06/01/99 9:11 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HUNCHED; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; ROUGH HAIR COAT; -DILATATION, VENTRICULAR,-PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE LIVER (LI) : >SKIN & PELAGE; RED HAIR COAT; VENTRAL- -VACUOLATION, HEPATOCELLULAR,-SEVERE, ABDOMINAL; VERIFIED AT NECROPSY: NOT MULTI-FOCAL OBSERVED ADRENAL, CORTEX (AC) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -VACUOLATION,-MINIMAL, FOCAL NECROPSY: NOT APPLICABLE PITUITARY (PI) : PITUITARY (PI) : >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -MASS(ES); 10 X 8 X 8 MM, RED, DARK -HEMORRHAGE,-MODERATELY SEVERE, MULTI- AT NECROPSY: NOT APPLICABLE RED, GREY AND SOFT FOCAL >APPEARANCE; THIN; VERIFIED AT -B-ADENOMA,-NEOPLASM PRESENT NECROPSY: NOT OBSERVED STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : >EXCRETION; NO FECES; VERIFIED AT -EROSION/ULCERATION; SINGLE ULCERATED -EDEMA,-MODERATELY SEVERE, FOCAL NECROPSY: NOT APPLICABLE AREA, 4 MM IN DIAMETER; ADHERED TO -EROSION,-MODERATELY SEVERE, MULTI- OUTLYING ADIPOSE TISSUE FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,- URETHRA: GREEN AND FIRM MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2266 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 665 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90962 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/01/99 STUDY DAY OF DEATH: 492 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 475.5 GRAMS DATE AND TIME OF NECROPSY: 06/01/99 9:11 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; ENTIRE VENTRAL SURFACE: YELLOW AND BROWN -BLOOD FILM TAKEN -DISCHARGE-NASAL; BILATERAL: RED -------------------------------------------- 2267 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 666 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90962 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/01/99 STUDY DAY OF DEATH: 492 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 475.5 GRAMS DATE AND TIME OF NECROPSY: 06/01/99 9:11 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2268 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 667 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90963 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 412.7 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 15:25 PROSECTOR: NOT AVAILABLE RECORDER: NOT AVAILABLE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: NOT AVAILABLE WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; ROUGH HAIR COAT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -DIFFUSELY DARK; ALL LOBES: BROWN -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >SKIN & PELAGE; YELLOW HAIR COAT; -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- VERIFIED AT NECROPSY: NOT OBSERVED FOCAL >SKIN & PELAGE; COLD TO TOUCH; ENTIRE KIDNEY (KD) : BODY; VERIFIED AT NECROPSY: NOT -CAST, PROTEINACEOUS,-MINIMAL, MULTI- APPLICABLE FOCAL >BEHAVIOR; ATAXIC; VERIFIED AT -PYELONEPHRITIS,-MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE ADRENAL, CORTEX (AC) : >APPEARANCE; HUNCHED; VERIFIED AT -VACUOLATION,-MINIMAL, FOCAL NECROPSY: NOT APPLICABLE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, RED, DARK -HEMORRHAGE,-MINIMAL, MULTI-FOCAL RED, TAN AND FIRM -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : HARDERIAN GLAND (HG) : >NOTE:>TRIMMING COMMENT: MISSING FROM >TISSUE MISSING WET TISSUE STOMACH, NONGL (SU) : -EDEMA,-MODERATE, FOCAL 2269 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 668 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90963 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 412.7 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 15:25 PROSECTOR: NOT AVAILABLE RECORDER: NOT AVAILABLE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: NOT AVAILABLE WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: -EROSION,-MINIMAL, FOCAL MULTIPLE TAN, HARD AND SLIGHTLY RAISED -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, AREAS, UP TO 3 X 2 MM FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2270 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 669 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90963 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 412.7 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 15:25 PROSECTOR: NOT AVAILABLE RECORDER: NOT AVAILABLE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: NOT AVAILABLE WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2271 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 670 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90964 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/24/99 STUDY DAY OF DEATH: 515 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 489.0 GRAMS DATE AND TIME OF NECROPSY: 06/24/99 15:14 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; EYE (EY) : EYE (EY) : VERIFIED AT NECROPSY: OBSERVED -OPAQUE CORNEA; RIGHT -INFLAMMATION, ACUTE, CORNEAL,-MINIMAL, >APPEARANCE; SWOLLEN; PAWS-HIND; MULTI-FOCAL VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, CORNEAL,-MINIMAL, >EYES; OPAQUE; EYE-RIGHT; VERIFIED AT MULTI-FOCAL NECROPSY: OBSERVED LIVER (LI) : >EYES; DISCOLORED/LIGHT RED; EYE-LEFT; -CYST,-SLIGHT, MULTI-FOCAL VERIFIED AT NECROPSY: NOT OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >APPEARANCE; THIN; VERIFIED AT EOSINOPHILIC,-MODERATE, FOCAL NECROPSY: OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- >SKIN & PELAGE; PALE; ENTIRE BODY; FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- -DIFFUSELY LIGHT; BILATERAL: TAN SEVERE, DIFFUSE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MODERATE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT 2272 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 671 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90964 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/24/99 STUDY DAY OF DEATH: 515 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 489.0 GRAMS DATE AND TIME OF NECROPSY: 06/24/99 15:14 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ BONE, FEMUR (FE) : -OSTEODYSTROPHY, FIBROUS,-MODERATE, MULTI-FOCAL NERVE, SCIATIC (SN) : >TISSUE MISSING PARATHYROID (PT) : -HYPERPLASIA,-MODERATELY SEVERE, DIFFUSE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL -MINERALIZATION,-MODERATE, MULTI-FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL CAUSE OF DEATH (CD) : -CHRONIC PROGRESSIVE NEPHROPATHY,- PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL, LUMBAR: EACH IS 5 X -DILATATION, SINUSOIDAL,-MODERATE, 5 X 9 MM (CAP 21) MULTI-FOCAL -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, MULTI-FOCAL MAMMARY, MALE (MM) : -PIGMENT,-SLIGHT, MULTI-FOCAL 2273 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 672 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90964 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/24/99 STUDY DAY OF DEATH: 515 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 489.0 GRAMS DATE AND TIME OF NECROPSY: 06/24/99 15:14 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL, -EDEMA,-MODERATE, MULTI-FOCAL HINDFEET: SINGLE MOIST, RED AND TAN -EROSION,-SEVERE, MULTI-FOCAL ABRADED AREA ON EACH, LEFT IS 8 X 11 -INFLAMMATION, ACUTE,-MODERATELY MM, RIGHT IS 13 X 19 MM; SURROUNDING SEVERE, MULTI-FOCAL TISSUE APPEARS SWOLLEN; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -YELLOWISH MUCOUS MEMBRANES; DIFFUSE: PALE -------------------------------------------- 2274 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 673 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90964 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/24/99 STUDY DAY OF DEATH: 515 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 489.0 GRAMS DATE AND TIME OF NECROPSY: 06/24/99 15:14 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), PANCREAS (PA), LN, MESENTERIC (MS), TESTIS (TE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2275 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 674 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90965 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 533.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:27 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -DIFFUSELY DARK; ALL LOBES: DARK BROWN -DEGENERATION, CYSTIC,-SLIGHT, FOCAL >SKIN & PELAGE; SORE/SCAB; PAW-HIND- -FOCUS, ALTERED HEPATOCELLULAR, LEFT; VERIFIED AT NECROPSY: OBSERVED EOSINOPHILIC,-SLIGHT, MULTI-FOCAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATELY SEVERE, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : KIDNEY (KD) : -DIFFUSELY DARK; BILATERAL: DARK BROWN >UNREMARKABLE PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- SLIGHT, FOCAL -VACUOLATION,-SLIGHT, FOCAL LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -HYPERPLASIA, TYPE II PNEUMOCYTE,- MULTIPLE GRAY AREAS, UP TO 10 X 9 MM MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL 2276 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 675 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90965 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 533.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:27 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: -EROSION,-MODERATE, FOCAL SINGLE DARK RED, TAN, DRY CRUSTED -FIBROSIS,-MODERATE, FOCAL AREA, 4 MM IN DIAMETER; COLLECTED -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2277 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 676 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90965 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 533.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:27 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2278 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 677 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90966 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 393.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:53 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 13.2827 3.3730 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2279 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 678 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90966 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 393.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:53 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2280 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 679 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90967 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 664.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:23 PROSECTOR: WENDY CHERRY RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0400 .0060 % .0166 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0375 .0056 % .0155 WEIGHT TAKEN BRAIN (BR) 2.4167 .3639 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.4323 .3663 % 1.0065 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.3103 .3479 % .9560 WEIGHT TAKEN LIVER (LI) 23.8000 3.5838 % 9.8481 WEIGHT TAKEN LUNG (LU) 2.4213 .3646 % 1.0019 WEIGHT TAKEN SPLEEN (SP) 1.0494 .1580 % .4342 WEIGHT TAKEN LEFT TESTIS (TE0) 1.7744 .2672 % .7342 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.7720 .2668 % .7332 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0205 .0031 % .0085 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0188 .0028 % .0078 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : UNVERIFIED AT NECROPSY -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATELY SEVERE, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL PANCREAS (PA) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL 2281 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 680 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90967 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 664.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:23 PROSECTOR: WENDY CHERRY RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 6 MM, DARK RED AND -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SOFT -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2282 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 681 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90967 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 664.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:23 PROSECTOR: WENDY CHERRY RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2283 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 682 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90968 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:35 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; LIVER (LI) : VERIFIED AT NECROPSY: NOT OBSERVED -DEGENERATION, CYSTIC,-MINIMAL, FOCAL >APPEARANCE; MALOCCLUSION; VERIFIED AT -HYPERTROPHY, CENTRILOBULAR, NECROPSY: OBSERVED HEPATOCELLULAR,-SLIGHT, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 20 X 13 X 8 -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE MM; BOTH ARE SOFT -MINERALIZATION,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, ACUTE,-MODERATE, MULTI- URETHRA: GREEN AND FIRM FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS 2284 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 683 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90968 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:35 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2285 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 684 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90969 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/10/99 STUDY DAY OF DEATH: 593 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/10/99 9:42 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL SPLEEN (SP) : SPLEEN (SP) : -LARGE; 88 X 21 X 19 MM; MARGINS -HEMATOPOIESIS, EXTRAMEDULLARY, APPEAR ROUNDED INCREASED,-SEVERE, DIFFUSE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 9 X 6 X 4 MM, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL RED, DARK RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: SINGLE >UNREMARKABLE DARK RED ERODED AREA, 3 MM IN DIAMETER PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: -EROSION,-MODERATELY SEVERE, FOCAL SINGLE DARK RED AND DRY CRUSTED AREA, -EXOSTOSES,-MODERATE, MULTI-FOCAL 6 MM IN DIAMETER; COLLECTED -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL -------------------------------------------- 2286 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 685 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90969 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/10/99 STUDY DAY OF DEATH: 593 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/10/99 9:42 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2287 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 686 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90970 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 639.0 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 13:46 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 56; 20 X 25MM; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; SORE/SCAB; DORSAL; -DILATATION, VENTRICULAR,-PRESENT VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; SORE/SCAB; PAW-HIND- -RED FOCUS(I)/AREA(S); ALL LOBES: -VACUOLATION, HEPATOCELLULAR,-SEVERE, LEFT; VERIFIED AT NECROPSY: OBSERVED MULTIPLE DARK RED AREAS, UP TO 6 MM IN DIFFUSE >BEHAVIOR; HYPOACTIVE; VERIFIED AT DIAMETER; COLLECTED ON ROUTINE NECROPSY: NOT APPLICABLE SECTIONS >RESPIRATION; IRREGULAR; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >EYES; SQUINTED; PERI-ORBITAL; VERIFIED FOCAL AT NECROPSY: NOT APPLICABLE PITUITARY (PI) : PITUITARY (PI) : >SKIN & PELAGE; COLD TO TOUCH; ENTIRE -MASS(ES); 8 X 8 X 7 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL BODY; VERIFIED AT NECROPSY: NOT TAN, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT APPLICABLE STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -EDEMA,-SLIGHT, FOCAL TAN ERODED AREAS, UP TO 2 MM IN -EROSION,-SLIGHT, FOCAL DIAMETER -INFLAMMATION, ACUTE,-SLIGHT, FOCAL SKIN (SK) : -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT 2288 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 687 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90970 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 639.0 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 13:46 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : -MASS(ES); MID DORSAL THORACIC REGION: SINGLE MASS, 40 X 35 X 35 MM, TAN, DARK RED AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN HAS A SINGLE DARK RED CRUSTED AREA, 20 MM IN DIAMETER; COLLECTED INTACT PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT HIND -EDEMA,-MODERATELY SEVERE, FOCAL FOOT: SINGLE DARK RED AND MOIST -EROSION,-MODERATELY SEVERE, FOCAL ABRADED AREA, 13 X 10 X 5 MM; -HEMORRHAGE,-MODERATE, MULTI-FOCAL COLLECTED -INFLAMMATION, ACUTE,-MODERATE, FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND MOIST -ANIMAL OBESE -------------------------------------------- 2289 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 688 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90970 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 639.0 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 13:46 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2290 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 689 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90971 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 262.0 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:17 PROSECTOR: TODD ACKER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0288 .0110 % .0165 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0200 .0076 % .0115 WEIGHT TAKEN BRAIN (BR) 1.7426 .6651 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.1749 .4484 % .6742 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.2073 .4608 % .6928 WEIGHT TAKEN LIVER (LI) 20.2788 7.7400 % 11.6371 WEIGHT TAKEN LUNG (LU) 1.0138 .3869 % .5818 WEIGHT TAKEN SPLEEN (SP) .4768 .1820 % .2736 WEIGHT TAKEN LEFT TESTIS (TE0) 1.6178 .6175 % .9284 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.5791 .6027 % .9062 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0112 .0043 % .0064 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0093 .0035 % .0053 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -HEMORRHAGE,-MINIMAL, MULTI-FOCAL MULTIPLE TAN AREAS, UP TO 14 X 2 MM; -HYPERTROPHY, CENTRILOBULAR, COLLECTED ON ROUTINE SECTION HEPATOCELLULAR,-MODERATE, DIFFUSE -LARGE; ALL LOBES -NECROSIS, HEPATOCELLULAR,-MODERATE, -DEPRESSED FOCUS(I)/AREA(S); MULTI-FOCAL DIAPHRAGMATIC SURFACE OF LEFT AND RIGHT MEDIAN LOBES: SINGLE, LINEAR, DEPRESSED AREA ON EACH, LEFT IS 15 X 2 MM, RIGHT IS 20 X 2 MM; COLLECTED ON ROUTINE SECTION AND EXTRA SECTION COLLECTED 2291 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 690 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90971 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 262.0 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:17 PROSECTOR: TODD ACKER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -DIFFUSELY RED; BILATERAL: DARK RED >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), DUODENUM (DU) ------------------------------------------------------------------------------------------------------------------------------------ 2292 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 691 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90972 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 301.3 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 9:50 PROSECTOR: DONIA MEYER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0184 .0061 % .0089 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0222 .0074 % .0107 WEIGHT TAKEN BRAIN (BR) 2.0763 .6891 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.2294 .4080 % .5921 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.3130 .4358 % .6324 WEIGHT TAKEN LIVER (LI) 18.5147 6.1449 % 8.9172 WEIGHT TAKEN LUNG (LU) 1.0329 .3428 % .4975 WEIGHT TAKEN SPLEEN (SP) .4625 .1535 % .2228 WEIGHT TAKEN LEFT TESTIS (TE0) 1.8046 .5989 % .8691 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.7682 .5869 % .8516 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0091 .0030 % .0044 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0076 .0025 % .0037 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LARGE; ALL LOBES -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -DIFFUSELY DARK; ALL LOBES: DARK BROWN -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: TWO >NOT REQUIRED TO BE EXAMINED FOR ANIMAL DARK BROWN FOCI, EACH IS 1 MM IN DIAMETER CECUM (CE) : CECUM (CE) : -DIFFUSELY RED; DIFFUSE >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 2293 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 692 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90972 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 301.3 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 9:50 PROSECTOR: DONIA MEYER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2294 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 693 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90984 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 328.4 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:24 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 16.3999 4.9939 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2295 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 694 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90984 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 328.4 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:24 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2296 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 695 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90988 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 296.7 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:50 PROSECTOR: GREGORY CODDINGTON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0276 .0093 % .0132 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0225 .0076 % .0108 WEIGHT TAKEN BRAIN (BR) 2.0831 .7021 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.3893 .4683 % .6669 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.3243 .4463 % .6357 WEIGHT TAKEN LIVER (LI) 19.8542 6.6917 % 9.5311 WEIGHT TAKEN LUNG (LU) 1.1918 .4017 % .5721 WEIGHT TAKEN SPLEEN (SP) .6098 .2055 % .2927 WEIGHT TAKEN LEFT TESTIS (TE0) 1.5892 .5356 % .7629 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.5609 .5261 % .7493 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0075 .0025 % .0036 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0076 .0026 % .0036 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LARGE; ALL LOBES -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -DIFFUSELY DARK; ALL LOBES: DARK BROWN -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE -NECROSIS, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -EDEMA; ADIPOSE TISSUE SURROUNDING >NOT REQUIRED TO BE EXAMINED FOR ANIMAL LEFT KIDNEY: CLEAR GELATINOUS AND EDEMATOUS (CAP 18) 2297 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 696 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90988 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 296.7 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:50 PROSECTOR: GREGORY CODDINGTON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2298 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 697 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90993 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/19/98 STUDY DAY OF DEATH: 53 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/19/98 16:23 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -LARGE; ALL LOBES: THICKENED -HEMORRHAGE,-MODERATELY SEVERE, MULTI- -MOTTLED; ALL LOBES: DARK BROWN AND FOCAL TAN -NECROSIS, HEPATOCELLULAR,-MODERATELY SEVERE, MULTI-FOCAL THYMUS (TH) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI- FOCAL LUNG (LU) : LUNG (LU) : -DIFFUSELY RED; ALL LOBES: DARK RED >UNREMARKABLE CAUSE OF DEATH (CD) : -HEPATIC NECROSIS,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-ORAL; RED AND DRY -DISCHARGE-OCULAR; BILATERAL: DARK RED AND CRUSTED -DISCHARGE-NASAL; RED AND DRY -MALOCCLUSION; BILATERAL UPPER AND LOWER INCISORS -GI TRACT-DARK FLUID CONTENT; ENTIRE GI TRACT: DARK BROWN, TENACIOUS SEMIFLUID -------------------------------------------- 2299 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 698 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90993 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/19/98 STUDY DAY OF DEATH: 53 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/19/98 16:23 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2300 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 699 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90996 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 397.0 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:08 PROSECTOR: GREGORY CODDINGTON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0322 .0081 % .0147 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0309 .0078 % .0141 WEIGHT TAKEN BRAIN (BR) 2.1902 .5517 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.7846 .4495 % .8148 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.9303 .4862 % .8813 WEIGHT TAKEN LIVER (LI) 27.3204 6.8817 % 12.4739 WEIGHT TAKEN LUNG (LU) 1.6624 .4187 % .7590 WEIGHT TAKEN SPLEEN (SP) .7825 .1971 % .3573 WEIGHT TAKEN LEFT TESTIS (TE0) 1.9606 .4939 % .8952 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.9131 .4819 % .8735 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0096 .0024 % .0044 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0108 .0027 % .0049 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LARGE; ALL LOBES -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE KIDNEY (KD) : -CYST,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 2301 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 700 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C90996 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 397.0 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:08 PROSECTOR: GREGORY CODDINGTON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2302 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 701 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91003 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/11/98 STUDY DAY OF DEATH: 45 STUDY WEEK OF DEATH: 7 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/11/98 11:40 PROSECTOR: NANCY DIEDRICH RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LARGE; ALL LOBES: THICKENED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -MOTTLED; ALL LOBES: RED, BROWN AND -HYPERTROPHY, CENTRILOBULAR, TAN HEPATOCELLULAR,-MODERATELY SEVERE, DIFFUSE -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL THYMUS (TH) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL LUNG (LU) : LUNG (LU) : -DIFFUSELY RED; ALL LOBES: DARK RED -HEMORRHAGE,-SLIGHT, MULTI-FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK BROWN FOCI, UP TO 1 MM IN DIAMETER CAUSE OF DEATH (CD) : -HEPATIC NECROSIS,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-ORAL; RED AND MOIST MATERIAL -DISCHARGE-NASAL; DARK RED AND MOIST MATERIAL -MALOCCLUSION; BILATERAL: UPPER INCISORS; UPPER LEFT INCISOR LOOSE AND EASILY MANIPULATED 2303 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 702 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91003 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/11/98 STUDY DAY OF DEATH: 45 STUDY WEEK OF DEATH: 7 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/11/98 11:40 PROSECTOR: NANCY DIEDRICH RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2304 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 703 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91005 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 295.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:25 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 15.6214 5.2793 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2305 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 704 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91005 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 295.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:25 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2306 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 705 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91017 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 329.5 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 9:45 PROSECTOR: TODD ACKER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0318 .0097 % .0148 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0347 .0105 % .0162 WEIGHT TAKEN BRAIN (BR) 2.1473 .6517 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.4601 .4431 % .6800 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.6177 .4910 % .7534 WEIGHT TAKEN LIVER (LI) 22.0840 6.7023 % 10.2845 WEIGHT TAKEN LUNG (LU) 1.3642 .4140 % .6353 WEIGHT TAKEN SPLEEN (SP) .6191 .1879 % .2883 WEIGHT TAKEN LEFT TESTIS (TE0) 1.7923 .5439 % .8347 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.8783 .5700 % .8747 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0015 .0005 % .0007 EXCLUDE RIGHT THYROID/PR (TT1) .0155 .0047 % .0072 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LARGE; ALL LOBES -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: SINGLE >NOT REQUIRED TO BE EXAMINED FOR ANIMAL DARK RED FOCUS, 1 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 2307 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 706 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91017 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 329.5 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 9:45 PROSECTOR: TODD ACKER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2308 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 707 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91024 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 268.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:37 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 13.4285 4.9957 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2309 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 708 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91024 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 268.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:37 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2310 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 709 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91029 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/15/98 STUDY DAY OF DEATH: 49 STUDY WEEK OF DEATH: 7 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/15/98 9:06 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; CONVULSIONS; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -HYPERTROPHY, CENTRILOBULAR, >SKIN & PELAGE; RED HAIR COAT; HEAD- HEPATOCELLULAR,-MODERATELY SEVERE, ENTIRE; VERIFIED AT NECROPSY: NOT DIFFUSE OBSERVED -NECROSIS, HEPATOCELLULAR,-SLIGHT, >BEHAVIOR; VOCALIZATION; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE THYMUS (TH) : THYMUS (TH) : >SACRIFICE; SACRIFICE APPROVED BY; -RED FOCUS(I)/AREA(S); DIFFUSE: -HEMORRHAGE,-MINIMAL, MULTI-FOCAL STUDY DIRECTOR; VERIFIED AT NECROPSY: MULTIPLE DARK RED AREAS, UP TO 3 X 1 NOT APPLICABLE MM >RESPIRATION; LABORED; VERIFIED AT THYROID (TY) : NECROPSY: NOT APPLICABLE -CYST, ULTIMOBRANCHIAL,-PRESENT >DISCHARGE; UNKNOWN; RED IN COLOR; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -UNDETERMINED,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; RED, DRY MATERIAL -BLOOD FILM TAKEN -------------------------------------------- 2311 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 710 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91029 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/15/98 STUDY DAY OF DEATH: 49 STUDY WEEK OF DEATH: 7 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/15/98 9:06 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2312 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 711 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91033 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 316.6 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:46 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 16.2783 5.1416 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2313 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 712 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91033 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 316.6 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:46 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2314 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 713 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91037 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 340.5 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:18 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 17.0914 5.0195 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2315 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 714 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91037 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 340.5 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:18 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2316 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 715 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91041 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/22/99 STUDY DAY OF DEATH: 421 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 440.0 GRAMS DATE AND TIME OF NECROPSY: 03/22/99 14:20 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; BROWN HAIR COAT; BRAIN (BR) : BRAIN (BR) : VENTRAL-ABDOMINAL; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR,- >EYES; SQUINTED; PERI-ORBITAL; VERIFIED MODERATELY SEVERE, DIFFUSE AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : KIDNEY (KD) : >SKIN & PELAGE; RED HAIR COAT; VENTRAL- -ROUGH SURFACE, DIFFUSE; BILATERAL -CAST, PROTEINACEOUS,-MINIMAL, MULTI- CERVICAL; VERIFIED AT NECROPSY: FOCAL OBSERVED ADRENAL, CORTEX (AC) : >SKIN & PELAGE; ROUGH HAIR COAT; -ANGIECTASIS,-MINIMAL, FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE -HEMORRHAGE,-MINIMAL, FOCAL >APPEARANCE; THIN; VERIFIED AT PITUITARY (PI) : PITUITARY (PI) : NECROPSY: OBSERVED -MASS(ES); SINGLE MASS, 6 X 9 X 11 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL FIRM, RED, AND TAN -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -EDEMA,-SLIGHT, FOCAL BROWN ERODED AREAS, UP TO 2 X 3 MM -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2317 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 716 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91041 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/22/99 STUDY DAY OF DEATH: 421 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 440.0 GRAMS DATE AND TIME OF NECROPSY: 03/22/99 14:20 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MEDIASTINAL (ML) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION MAMMARY, MALE (MM) : -PIGMENT,-SLIGHT, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; DRY RED MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DRY RED AND BROWN MATERIAL -ANIMAL THIN -------------------------------------------- 2318 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 717 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91041 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/22/99 STUDY DAY OF DEATH: 421 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 440.0 GRAMS DATE AND TIME OF NECROPSY: 03/22/99 14:20 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2319 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 718 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91042 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/06/99 STUDY DAY OF DEATH: 650 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/06/99 13:59 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- KIDNEY (KD) : LEFT; VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : PANCREAS (PA) : -MASS(ES); ATTACHED TO CRANIAL END: -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL SINGLE DARK RED AND FIRM MASS, 4 MM IN -B-ADENOMA, ISLET CELL,-NEOPLASM DIAMETER; SAME ON CUT SURFACE (CAP PRESENT 23); REGIONAL LN (PANCREATIC) NOT IDENTIFIED ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL LUNG (LU) : LUNG (LU) : -UNCOLLAPSED; ALL LOBES -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL MUSCLE, SKELETAL (SM) : -DEGENERATION/NECROSIS,-MODERATE, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 4 X 4 X 3 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT 2320 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 719 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91042 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/06/99 STUDY DAY OF DEATH: 650 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/06/99 13:59 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE BLACK AREAS, UP TO 2 X 1 MM CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); BILATERAL PECTORAL REGIONS: -GALACTOCELE,-MODERATE, MULTI-FOCAL MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 X 2 MM (CAP 22) FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: -EDEMA,-MODERATE, FOCAL SINGLE DRY, TAN CRUSTED AREA, 2 X 1 -EROSION,-MODERATE, FOCAL MM; COLLECTED -INFLAMMATION, ACUTE,-MODERATE, FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -STAINS - SKIN/HAIRCOAT; ALL REGIONS, DIFFUSE: DRY, RED MATERIAL -------------------------------------------- 2321 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 720 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91042 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/06/99 STUDY DAY OF DEATH: 650 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/06/99 13:59 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2322 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 721 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91043 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 940.8 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 13:47 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-RIGHT; WEEK LIVER (LI) : 75; 80 X 80MM; VERIFIED AT NECROPSY: -DEGENERATION, CYSTIC,-MINIMAL, FOCAL OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL MUSCLE, SKELETAL (SM) : MUSCLE, SKELETAL (SM) : -MASS(ES); INVOLVING ENTIRE RIGHT LEG: -CYST,-PRESENT SINGLE MASS, 80 MM IN DIAMETER, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED INTACT WITH LEG; REGIONAL LN (INGUINAL) NOT IDENTIFIED PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2323 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 722 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91043 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 940.8 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 13:47 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL OBESE -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2324 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 723 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91044 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/13/99 STUDY DAY OF DEATH: 443 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 368.5 GRAMS DATE AND TIME OF NECROPSY: 04/13/99 11:38 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; HUNCHED; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -DIFFUSELY DARK; ALL LOBES: DARK BROWN -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >APPEARANCE; HEAD TILT; RIGHT; VERIFIED KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 17 X 12 X 5 -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE MM; BOTH ARE SOFT THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 6 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- BROWN, TAN, AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 2325 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 724 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91044 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/13/99 STUDY DAY OF DEATH: 443 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 368.5 GRAMS DATE AND TIME OF NECROPSY: 04/13/99 11:38 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN AREA, 2 X 1 MM STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE >UNREMARKABLE BROWN, SLIGHTLY RAISED AREA, 1 MM IN DIAMETER PROSTATE (PR) : -MINERALIZATION, LUMINAL,-MODERATE, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS UP TO 12 X >UNREMARKABLE 4 X 2 MM EPIDIDYMIDES (EP) : EPIDIDYMIDES (EP) : -SMALL; BILATERAL: EACH IS 35 X 10 X 3 -HYPOSPERMIA,-PRESENT MM; BOTH ARE SOFT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -PIGMENT,-SLIGHT, MULTI-FOCAL >NOTE:>MAMMARY GLAND WAS PRESENT ON SKIN SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DARK RED, DRY, CRUSTED MATERIAL -DISCHARGE-NASAL; RED, DRY MATERIAL -ANIMAL THIN -------------------------------------------- 2326 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 725 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91044 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/13/99 STUDY DAY OF DEATH: 443 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 368.5 GRAMS DATE AND TIME OF NECROPSY: 04/13/99 11:38 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2327 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 726 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91045 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/13/99 STUDY DAY OF DEATH: 353 STUDY WEEK OF DEATH: 51 TERMINAL BODY WEIGHT: 535.8 GRAMS DATE AND TIME OF NECROPSY: 01/13/99 9:30 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >SKIN & PELAGE; BROWN HAIR COAT; -VACUOLATION, HEPATOCELLULAR,-MODERATE, VENTRAL-ABDOMINAL; VERIFIED AT MULTI-FOCAL NECROPSY: NOT OBSERVED KIDNEY (KD) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE MINIMAL, MULTI-FOCAL THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 8 X 8 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE BROWN AREAS, UP TO 5 X 1 MM PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2328 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 727 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91045 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/13/99 STUDY DAY OF DEATH: 353 STUDY WEEK OF DEATH: 51 TERMINAL BODY WEIGHT: 535.8 GRAMS DATE AND TIME OF NECROPSY: 01/13/99 9:30 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DARK RED AND DRY MATERIAL -------------------------------------------- 2329 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 728 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91045 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/13/99 STUDY DAY OF DEATH: 353 STUDY WEEK OF DEATH: 51 TERMINAL BODY WEIGHT: 535.8 GRAMS DATE AND TIME OF NECROPSY: 01/13/99 9:30 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2330 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 729 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91046 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/98 STUDY DAY OF DEATH: 44 STUDY WEEK OF DEATH: 7 TERMINAL BODY WEIGHT: 358.1 GRAMS DATE AND TIME OF NECROPSY: 03/10/98 8:05 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >RESPIRATION; IRREGULAR; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -HYPERTROPHY, CENTRILOBULAR, >EYES; RED DISCHARGE; EYE-LEFT; MULTIPLE TAN AREAS, UP TO 7 X 2 MM; HEPATOCELLULAR,-MODERATE, DIFFUSE VERIFIED AT NECROPSY: OBSERVED COLLECTED ON ROUTINE SECTIONS -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL THYMUS (TH) : THYMUS (TH) : -RED FOCUS(I)/AREA(S); DIFFUSE: -HEMORRHAGE,-MINIMAL, MULTI-FOCAL MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM LUNG (LU) : LUNG (LU) : -DIFFUSELY RED; ALL LOBES: DARK RED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -HYPERPLASIA, TYPE II PNEUMOCYTE,- SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -HEPATIC NECROSIS,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; -HEMORRHAGE,-MINIMAL, MULTI-FOCAL COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DRY RED MATERIAL -------------------------------------------- 2331 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 730 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91046 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/98 STUDY DAY OF DEATH: 44 STUDY WEEK OF DEATH: 7 TERMINAL BODY WEIGHT: 358.1 GRAMS DATE AND TIME OF NECROPSY: 03/10/98 8:05 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2332 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 731 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91047 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 677.5 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:45 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -DEGENERATION, CYSTIC,-SLIGHT, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, FOCAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2333 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 732 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91047 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 677.5 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:45 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2334 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 733 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91048 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 490.5 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 13:59 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; BROWN HAIR COAT; -DILATATION, VENTRICULAR,-PRESENT GENERALIZED; VERIFIED AT NECROPSY: LIVER (LI) : OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- >EYES; SQUINTED; PERI-ORBITAL; VERIFIED FOCAL AT NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR,-MODERATE, >BEHAVIOR; HYPOACTIVE; VERIFIED AT MULTI-FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >EXCRETION; NO FECES; VERIFIED AT -INFARCT,-MINIMAL, FOCAL NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL -PYELONEPHRITIS,-MODERATE, MULTI-FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 5 X 6 X 8 MM, -HEMORRHAGE,-MINIMAL, MULTI-FOCAL SOFT, RED, DARK RED, AND TAN -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 2335 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 734 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91048 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 490.5 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 13:59 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BROWN FOCI, PINPOINT AND UP TO 1 MM IN DIAMETER STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -EDEMA,-MODERATE, FOCAL TAN ERODED AREAS, UP TO 1 X 2 MM -INFLAMMATION, ACUTE,-MINIMAL, MULTI- -THICKENED WALL; DIFFUSE FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; DRY BROWN MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DRY BROWN MATERIAL -ANIMAL THIN -------------------------------------------- 2336 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 735 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91048 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 490.5 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 13:59 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2337 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 736 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91049 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/16/98 STUDY DAY OF DEATH: 295 STUDY WEEK OF DEATH: 43 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/16/98 12:27 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >APPEARANCE; SWOLLEN; VENTRAL- FOCAL ABDOMINAL; VERIFIED AT NECROPSY: ADRENAL, CORTEX (AC) : OBSERVED -VACUOLATION,-MINIMAL, MULTI-FOCAL >RESPIRATION; IRREGULAR; VERIFIED AT ADRENAL, MEDULLA (MA) : NECROPSY: NOT APPLICABLE -VACUOLATION,-MINIMAL, MULTI-FOCAL >SKIN & PELAGE; ROUGH HAIR COAT; LN, MESENTERIC (MS) : VERIFIED AT NECROPSY: NOT APPLICABLE -HEMORRHAGE,-MINIMAL, MULTI-FOCAL >SKIN & PELAGE; YELLOW HAIR COAT; THYMUS (TH) : VERIFIED AT NECROPSY: NOT OBSERVED -DEPLETION, LYMPHOCYTIC,-MODERATELY >DISCHARGE; NASAL; RED IN COLOR; SEVERE, DIFFUSE VERIFIED AT NECROPSY: OBSERVED PROSTATE (PR) : >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED -INFLAMMATION, CHRONIC-ACTIVE,- AT NECROPSY: NOT APPLICABLE MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -NECROSIS, ANAL MUSCULATURE,-PRESENT CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE -NECROSIS, FAT,-PRESENT NEAR LEFT TESTIS: SINGLE MASS, 15 X 7 X 4 MM, RED, TAN, AND SOFT; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (LUMBAR) NORMAL (CAP 21) 2338 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 737 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91049 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/16/98 STUDY DAY OF DEATH: 295 STUDY WEEK OF DEATH: 43 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/16/98 12:27 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ANUS (AN) : ANUS (AN) : -MASS(ES); WITHIN LUMEN: SINGLE MASS, -NECROSIS, MUSCULAR,-MODERATELY SEVERE, 12 MM IN DIAMETER, RED, GRAY, TAN AND MULTI-FOCAL FIRM; SAME ON CUT SURFACE; COLLECTED INTACT WITH RECTUM; REGIONAL LN (LUMBAR) NORMAL (CAP 21) GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BROWN AND DRY -DISCHARGE-NASAL; DARK RED AND DRY MATERIAL -GI TRACT-LARGE LUMEN; JEJUNUM TO ANUS: UP TO 40 MM IN DIAMETER -GI TRACT-DARK DRY CONTENT; RECTUM TO ANUS: DARK GREEN AND IMPACTED MATERIAL -------------------------------------------- 2339 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 738 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91049 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/16/98 STUDY DAY OF DEATH: 295 STUDY WEEK OF DEATH: 43 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/16/98 12:27 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 2340 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 739 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91050 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/14/00 15:56 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT EYE (EY) : NECROPSY: OBSERVED -INFLAMMATION, ACUTE, CORNEAL,-SLIGHT, >WART LIKE LESION(S); DORSAL; WEEK 95; FOCAL VERIFIED AT NECROPSY: OBSERVED -VASCULARIZATION, CORNEAL,-SLIGHT, FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT -CALCULUS(I); BILATERAL, WITHIN -MINERALIZATION, PELVIC,-SEVERE, MULTI- PELVIS: MULTIPLE TAN AND FIRM CALCULI, FOCAL UP TO 10 MM IN DIAMETER -NECROSIS, PAPILLARY,-MODERATELY SEVERE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL -PYELONEPHRITIS,-MODERATELY SEVERE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL -INFLAMMATION, VASCULAR,-MODERATE, MULTI-FOCAL LUNG (LU) : -EDEMA, ALVEOLAR,-MODERATE, FOCAL 2341 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 740 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91050 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/14/00 15:56 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -INFLAMMATION, VASCULAR,-MODERATE, FOCAL PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT, DIFFUSE STOMACH, GL (ST) : -EDEMA,-MODERATE, FOCAL SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); MID DORSAL THORACIC -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM REGION: SINGLE TAN AND RAISED AREA, 7 PRESENT X 6 X 4 MM (CAP 22) URINARY BLADDER (UB) : URINARY BLADDER (UB) : -CALCULUS(I); WITHIN LUMEN: SINGLE TAN -HEMORRHAGE,-MODERATE, MULTI-FOCAL AND FIRM CALCULUS, 6 MM IN DIAMETER -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- -DIFFUSELY RED; DARK RED FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -UROLITHIASIS,-PRESENT LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; BILATERAL: EACH IS UP TO 15 X >UNREMARKABLE 10 X 7 MM (CAP 24) 2342 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 741 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91050 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/14/00 15:56 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TAIL (TI) : TAIL (TI) : -RAISED AREA(S); ALONG ENTIRE LENGTH: -CYST, EPIDERMAL INCLUSION,-PRESENT MULTIPLE DARK RED AREAS, UP TO 5 MM IN DIAMETER; COLLECTED URETER (UE) : URETER (UE) : -LARGE; BILATERAL: EACH IS UP TO 2 MM -DILATATION,-MODERATE, DIFFUSE IN DIAMETER (CAP 25) -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BROWN AND DRY -MALOCCLUSION; BILATERAL, UPPER INCISORS -URINARY BLADDER-LARGE LUMEN; 50 X 40 X 40 MM -------------------------------------------- 2343 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 742 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91050 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/14/00 15:56 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2344 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 743 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91051 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 482.3 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 15:14 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; NO FECES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -DEGENERATION, CYSTIC,-SLIGHT, FOCAL NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR,-SLIGHT, >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED MULTI-FOCAL AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >SKIN & PELAGE; PALE; ENTIRE BODY; -NEPHROPATHY, CHRONIC PROGRESSIVE,- VERIFIED AT NECROPSY: NOT APPLICABLE SLIGHT, MULTI-FOCAL >APPEARANCE; THIN; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: OBSERVED -DEGENERATION, CYSTIC, CORTICAL,- MINIMAL, FOCAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 18 X 10 X 3 -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE MM; BOTH APPEAR SOFT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL HEART (HT) : -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 7 X 6 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, DARK RED, TAN, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT 2345 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 744 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91051 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 482.3 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 15:14 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE -INFLAMMATION, ACUTE,-MODERATE, MULTI- DARK RED AND TAN ERODED AREA, 10 X 3 FOCAL MM -ULCERATION,-SEVERE, FOCAL -THICKENED WALL; DIFFUSE PROSTATE (PR) : -INFLAMMATION, CHRONIC,-MODERATE, MULTI- FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 12 X 8 X 1 >UNREMARKABLE MM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL -EROSION,-MODERATELY SEVERE, FOCAL HINDFEET: SINGLE DARK RED, ABRADED -EXOSTOSES,-MINIMAL, MULTI-FOCAL AREA ON EACH, LEFT IS 5 X 3 MM, RIGHT -FIBROSIS,-MODERATELY SEVERE, MULTI- IS 7 MM IN DIAMETER; BOTH COLLECTED FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; PERIANAL: BROWN AND MOIST -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2346 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 745 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91051 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 482.3 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 15:14 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2347 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 746 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91052 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/10/99 STUDY DAY OF DEATH: 470 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 597.7 GRAMS DATE AND TIME OF NECROPSY: 05/10/99 13:32 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; ROUGH HAIR COAT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; SORE/SCAB; PAW-HIND- -DILATATION, VENTRICULAR,-PRESENT LEFT; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : >SKIN & PELAGE; YELLOW HAIR COAT; -VACUOLATION, HEPATOCELLULAR,-MINIMAL, VERIFIED AT NECROPSY: NOT OBSERVED MULTI-FOCAL >APPEARANCE; HEAD TILT; RIGHT; VERIFIED KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -PYELONEPHRITIS,-MODERATE, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 10 X 10 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HIND FOOT: -INFLAMMATION, CHRONIC ACTIVE,-SLIGHT, SINGLE RED, DRY, CRUSTED AREA, 4 X 3 FOCAL MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2348 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 747 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91052 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/10/99 STUDY DAY OF DEATH: 470 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 597.7 GRAMS DATE AND TIME OF NECROPSY: 05/10/99 13:32 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2349 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 748 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91053 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/28/99 STUDY DAY OF DEATH: 580 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/28/99 14:45 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT OBSERVED -DIFFUSELY LIGHT; ALL LOBES: TAN >UNREMARKABLE >HOUSING,FOOD,WATER & MAINT.; TEETH KIDNEY (KD) : KIDNEY (KD) : CUT; VERIFIED AT NECROPSY: NOT -DIFFUSELY LIGHT; BILATERAL: TAN -MINERALIZATION, PELVIC,-MODERATE, APPLICABLE MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL LUNG (LU) : LUNG (LU) : -MOTTLED; ALL LOBES: RED, DARK RED, >UNREMARKABLE TAN AND DARK BROWN THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL JEJUNUM (JE) : >AUTOLYTIC CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-NASAL; MOIST BROWN MATERIAL -------------------------------------------- 2350 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 749 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91053 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/28/99 STUDY DAY OF DEATH: 580 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/28/99 14:45 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2351 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 750 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91054 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1042.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:47 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; SORE/SCAB; VENTRAL- LIVER (LI) : LIVER (LI) : ABDOMINAL; VERIFIED AT NECROPSY: -MOTTLED; ALL LOBES: RED AND BROWN -FOCUS, ALTERED HEPATOCELLULAR, OBSERVED EOSINOPHILIC,-MODERATE, FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -RAISED AREA(S); RIGHT, CORTICAL >TISSUE MISSING SURFACE: SINGLE DARK RED AREA, 2 MM IN >NOTE:>ADRENAL CORTICAL MASS DESCRIBED DIAMETER GROSSLY WAS A MALIGNANT PHEOCHROMOCYTOMA OF THE ADRENAL MEDULLA ADRENAL, MEDULLA (MA) : -M-MALIGNANT PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYROID (TY) : -CYST, FOLLICULAR,-MINIMAL, MULTI-FOCAL -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 5 X 3 MM, RED, TAN AND -HEMORRHAGE,-MODERATE, MULTI-FOCAL SOFT -B-ADENOMA,-NEOPLASM PRESENT 2352 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 751 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91054 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1042.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:47 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); VENTRAL ABDOMINAL -ACUTE INFLAMMATORY EXUDATE,-SLIGHT, REGION EXTENDING TO LEFT LATERAL MULTI-FOCAL LUMBAR REGIONS: MULTIPLE RED AND TAN -HEMORRHAGE,-SLIGHT, MULTI-FOCAL CRUSTED AREAS, UP TO 20 X 20 MM (CAP -ULCERATION,-MINIMAL, MULTI-FOCAL 22) PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- URETHRA: GREEN AND FIRM FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); ALL REGIONS, DIFFUSE: -GALACTOCELE,-MODERATE, MULTI-FOCAL MULTIPLE TAN, CREAMY SEMIFLUID FILLED -MINERALIZATION,-MINIMAL, MULTI-FOCAL CYSTS, UP TO 4 MM IN DIAMETER (CAP 23) FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: -EROSION,-MODERATELY SEVERE, MULTI- SINGLE RED, BROWN AND RAISED AREA, 10 FOCAL X 7 X 4 MM; COLLECTED -FIBROSIS,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL -PIGMENT,-SLIGHT, FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 2353 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 752 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91054 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1042.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:47 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), LUNG (LU), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2354 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 753 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91055 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/17/99 STUDY DAY OF DEATH: 569 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/17/99 14:19 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; YELLOW HAIR COAT; BRAIN (BR) : VERIFIED AT NECROPSY: NOT OBSERVED -HEMORRHAGE,-MODERATE, MULTI-FOCAL >EXCRETION; NO FECES; VERIFIED AT -NECROSIS, GRAY MATTER,-MODERATE, MULTI- NECROPSY: NOT OBSERVED FOCAL >SKIN & PELAGE; BROWN HAIR COAT; -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO VENTRAL-ABDOMINAL; VERIFIED AT NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; BROWN HAIR COAT; -LIGHT FOCUS(I)/AREA(S); LEFT LATERAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PERINEAL AREA; VERIFIED AT NECROPSY: LOBE, NEAR MARGIN: MULTIPLE TAN AREAS, -NECROSIS, HEPATOCELLULAR,-MODERATE, OBSERVED UP TO 1 MM IN DIAMETER; COLLECTED ON MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT ROUTINE SECTION -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NECROPSY: NOT APPLICABLE -ROUGH SURFACE, DIFFUSE; ALL LOBES NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT >EYES; SQUINTED; PERI-ORBITAL; VERIFIED KIDNEY (KD) : KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED -MINERALIZATION, TUBULAR,-SLIGHT, MULTI- AT NECROPSY: NOT APPLICABLE FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE, DIFFUSE ADRENAL, CORTEX (AC) : -NECROSIS, CORTICAL,-SLIGHT, FOCAL -VACUOLATION,-MODERATE, FOCAL SPINAL CORD (SC) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -NECROSIS,-SLIGHT, MULTI-FOCAL 2355 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 754 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91055 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/17/99 STUDY DAY OF DEATH: 569 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/17/99 14:19 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); DORSAL >UNREMARKABLE SURFACE OF RIGHT CAUDAL LOBE: MULTIPLE TAN AREAS, UP TO 3 MM IN DIAMETER PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EROSION,-SLIGHT, FOCAL MULTIPLE DARK BROWN AREAS, UP TO 3 X 1 MM STOMACH, NONGL (SU) : -EDEMA,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL -ULCERATION,-MODERATE, FOCAL CECUM (CE) : -HEMORRHAGE,-SLIGHT, FOCAL URINARY BLADDER (UB) : URINARY BLADDER (UB) : -DIFFUSELY RED; DARK RED -HEMORRHAGE,-SEVERE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL -ULCERATION,-SEVERE, MULTI-FOCAL PROSTATE (PR) : PROSTATE (PR) : -MOTTLED; DARK RED, BROWN AND TAN -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -MOTTLED; BROWN AND TAN >UNREMARKABLE CAUSE OF DEATH (CD) : -MALIGNANT LYMPHOMA,-PRESENT 2356 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 755 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91055 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/17/99 STUDY DAY OF DEATH: 569 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/17/99 14:19 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT GENERAL COMMENT (GC) : -DISCHARGE-ORAL; MOIST YELLOW MATERIAL -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMEN EXTENDING TO PERIANAL REGION: DRY AND BROWN MATERIAL -ANIMAL OBESE -URINARY BLADDER-LARGE LUMEN; 40 X 25 X 10 MM; FILLED WITH A DARK RED FLUID -------------------------------------------- 2357 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 756 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91055 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/17/99 STUDY DAY OF DEATH: 569 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/17/99 14:19 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP) ------------------------------------------------------------------------------------------------------------------------------------ 2358 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 757 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91056 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 728.9 GRAMS DATE AND TIME OF NECROPSY: 10/27/00 10:07 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, FOCAL >MASS(ES); HEAD; WEEK 102; 50 X 40MM; TESTIS (TE) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL LUNG (LU) : -FIBROSIS, PLEURAL/SUBPLEURAL,- MODERATE, FOCAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -DILATATION, SINUSOIDAL,-MODERATE, -LARGE; RIGHT: 12 X 6 X 5 MM (LEFT IS FOCAL 5 X 3 X 2 MM); COLLECTED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL AUDITORY SEB GL (AS) : AUDITORY SEB GL (AS) : -MASS(ES); RIGHT: SINGLE MASS, 45 X 40 -INFLAMMATION, ACUTE,-MODERATE, MULTI- X 40 MM, RED, DARK RED, TAN, FOCAL LOBULATED, AND FIRM; ON SECTION EXUDES -M-CARCINOMA,-NEOPLASM PRESENT A LIGHT GREEN SEMISOLID; COLLECTED; REGIONAL LN (SEE MANDIBULAR) 2359 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 758 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91056 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 728.9 GRAMS DATE AND TIME OF NECROPSY: 10/27/00 10:07 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; RIGHT UPPER INCISOR -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA) ------------------------------------------------------------------------------------------------------------------------------------ 2360 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 759 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91057 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/19/99 STUDY DAY OF DEATH: 510 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/19/99 7:16 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-SLIGHT, MULTI- FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : KIDNEY (KD) : -EROSION(S)/ULCERATION(S); RIGHT, -MINERALIZATION, PELVIC,-SLIGHT, MULTI- CORTICAL SURFACE: SINGLE TAN AND RED FOCAL ERODED AREA, 10 X 8 MM -PYELONEPHRITIS,-MINIMAL, FOCAL -B-LIPOMA,-NEOPLASM PRESENT TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL SPINAL CORD (SC) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -HYPERPLASIA, FOLLICULAR CELL,-MINIMAL, FOCAL 2361 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 760 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91057 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/19/99 STUDY DAY OF DEATH: 510 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/19/99 7:16 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 5 X 5 MM, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- DARK RED, TAN, AND SOFT FOCAL -PIGMENT,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 13 X 6 X 2 >UNREMARKABLE MM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT -------------------------------------------- 2362 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 761 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91057 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/19/99 STUDY DAY OF DEATH: 510 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/19/99 7:16 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2363 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 762 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91058 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 737.0 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 12:09 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >APPEARANCE; SWOLLEN; MOUTH; VERIFIED EOSINOPHILIC,-MODERATE, FOCAL AT NECROPSY: NOT OBSERVED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL >WART LIKE LESION(S); MOUTH; WEEK 83; -VACUOLATION, HEPATOCELLULAR,-MODERATE, VERIFIED AT NECROPSY: OBSERVED MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 4 X 3 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : SKIN (SK) : -MASS(ES); LEFT UPPER LIP: SINGLE TAN -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM AND FIRM WART-LIKE MASS, 2 MM IN PRESENT DIAMETER (CAP 21); REGIONAL LN (MANDIBULAR) NORMAL (CAP 22) PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,- URETHRA: GREEN AND FIRM MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MULTI-FOCAL 2364 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 763 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91058 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 737.0 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 12:09 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2365 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 764 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91059 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/24/99 STUDY DAY OF DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 526.6 GRAMS DATE AND TIME OF NECROPSY: 08/24/99 14:07 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >SKIN & PELAGE; BROWN HAIR COAT; -DEGENERATION, CYSTIC,-SLIGHT, FOCAL GENERALIZED; VERIFIED AT NECROPSY: -VACUOLATION, HEPATOCELLULAR,-SLIGHT, OBSERVED MULTI-FOCAL >EYES; SQUINTED; PERI-ORBITAL; VERIFIED KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >RESPIRATION; LABORED; VERIFIED AT SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 12 X 7 X 6 MM, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- RED, DARK RED, TAN, SOFT, AND FOCAL LOBULATED -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -EDEMA,-SEVERE, MULTI-FOCAL DARK BROWN ERODED AREAS, UP TO 3 X 2 -INFLAMMATION, CHRONIC-ACTIVE,- MM MODERATE, MULTI-FOCAL -THICKENED WALL; DIFFUSE -ULCERATION,-SEVERE, MULTI-FOCAL 2366 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 765 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91059 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/24/99 STUDY DAY OF DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 526.6 GRAMS DATE AND TIME OF NECROPSY: 08/24/99 14:07 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: BROWN AND MOIST -------------------------------------------- 2367 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 766 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91059 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/24/99 STUDY DAY OF DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 526.6 GRAMS DATE AND TIME OF NECROPSY: 08/24/99 14:07 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2368 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 767 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91060 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 809.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 12:49 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYE-RIGHT; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -INFARCT,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DRY RED MATERIAL -BONE MARROW SMEAR TAKEN -------------------------------------------- 2369 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 768 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91060 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 809.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 12:49 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), TESTIS (TE), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2370 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 769 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91061 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/14/99 STUDY DAY OF DEATH: 627 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/14/99 8:06 PROSECTOR: JARED FISHEL RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK LIVER (LI) : 87; 40 X 50; VERIFIED AT NECROPSY: -DEGENERATION, CYSTIC,-MODERATE, MULTI- OBSERVED FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, MULTI-FOCAL LUNG (LU) : LUNG (LU) : -RED FOCUS(I)/AREA(S); ALONG MARGINS -HEMORRHAGE,-MODERATE, MULTI-FOCAL OF ALL LOBES: MULTIPLE DARK RED AND -HYPERPLASIA, TYPE II PNEUMOCYTE,- NODULAR AREAS, UP TO 20 X 5 MM MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI- FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MODERATE, MULTI-FOCAL SKIN (SK) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -B-HEMANGIOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -HEMANGIOMA,-PRESENT MAMMARY, MALE (MM) : -PIGMENT,-MINIMAL, MULTI-FOCAL 2371 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 770 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91061 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/14/99 STUDY DAY OF DEATH: 627 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/14/99 8:06 PROSECTOR: JARED FISHEL RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, MALE (MM) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 60 X 50 X 35 MM, RED, DARK RED, BROWN AND SOFT; SOME SECTIONS EXUDE A DARK RED FLUID; COLLECTED; REGIONAL LN (AXILLARY) NOT IDENTIFIED TAIL (TI) : TAIL (TI) : -DIFFUSELY DARK; INVOLVING ENTIRE TIP: -NECROSIS,-SEVERE, DIFFUSE BLACK; OVERLYING SKIN IS DRY, FIRM, CRUSTED AND RIGID; COLLECTED GENERAL COMMENT (GC) : -FLUID-THORACIC CAVITY; LARGE AMOUNT OF DARK RED FLUID -------------------------------------------- 2372 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 771 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91061 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/14/99 STUDY DAY OF DEATH: 627 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/14/99 8:06 PROSECTOR: JARED FISHEL RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2373 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 772 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91062 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 332.6 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 11:20 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -LIGHT FOCUS(I)/AREA(S); VISCERAL -FIBROSIS,-MODERATE, FOCAL NECROPSY: NOT APPLICABLE SURFACE OF LEFT LATERAL LOBE: SINGLE -HEMORRHAGE,-MODERATE, FOCAL >APPEARANCE; HEAD TILT; LEFT; VERIFIED TAN FOCUS, 2 MM IN DIAMETER; COLLECTED AT NECROPSY: NOT APPLICABLE ON ROUTINE SECTION >SKIN & PELAGE; PALE; ENTIRE BODY; KIDNEY (KD) : VERIFIED AT NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >APPEARANCE; HUNCHED; VERIFIED AT MODERATE, MULTI-FOCAL NECROPSY: NOT APPLICABLE ADRENAL, MEDULLA (MA) : >SKIN & PELAGE; BROWN HAIR COAT; -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PERINEAL AREA; VERIFIED AT NECROPSY: TESTIS (TE) : TESTIS (TE) : OBSERVED -SMALL; BILATERAL: EACH IS 16 X 9 X 5 -MINERALIZATION, VASCULAR,-MINIMAL, MM; BOTH APPEAR SOFT MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL -CYST, ULTIMOBRANCHIAL,-PRESENT -HEMORRHAGE,-SLIGHT, FOCAL -HYPERPLASIA, C-CELL,-MODERATELY SEVERE, FOCAL 2374 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 773 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91062 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 332.6 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 11:20 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 7 MM, RED, DARK -HEMORRHAGE,-SLIGHT, MULTI-FOCAL RED, TAN AND SOFT -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : PROSTATE (PR) : -SMALL; 9 X 8 X 5 MM -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 12 X 9 X 2 >UNREMARKABLE MM EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; PERINEUM: BROWN, MOIST MATERIAL -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2375 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 774 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91062 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 332.6 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 11:20 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2376 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 775 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91063 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 324.1 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 8:35 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE ADRENAL, CORTEX (AC) : >SKIN & PELAGE; BROWN HAIR COAT; -DEGENERATION, CYSTIC, CORTICAL,- VENTRAL-ABDOMINAL; VERIFIED AT SLIGHT, FOCAL NECROPSY: OBSERVED -VACUOLATION,-MODERATE, FOCAL >EYES; PALE; EYES; VERIFIED AT TESTIS (TE) : TESTIS (TE) : NECROPSY: NOT APPLICABLE -SMALL; LEFT: 17 X 9 X 4 MM (RIGHT IS -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE 20 X 13 X 10 MM); LEFT IS SOFT -GRANULOMA, SPERM,-MODERATE, MULTI- FOCAL -MINERALIZATION,-MODERATE, MULTI-FOCAL >NOTE:>LEFT TESTIS LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, MACROPHAGE, ALVEOLAR,- MULTIPLE TAN AREAS, UP TO 3 MM IN SLIGHT, MULTI-FOCAL DIAMETER PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 13 X 10 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT PROSTATE (PR) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -MINERALIZATION, LUMINAL,-SLIGHT, MULTI- FOCAL 2377 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 776 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91063 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 324.1 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 8:35 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 8 X 3 >UNREMARKABLE MM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL REGION: BROWN AND DRY -ANIMAL THIN -------------------------------------------- 2378 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 777 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91063 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 324.1 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 8:35 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2379 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 778 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91064 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/05/99 STUDY DAY OF DEATH: 679 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/05/99 14:14 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTIPLE DARK RED AREAS, UP TO 15 X 7 FOCAL MM; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : PARATHYROID (PT) : -LARGE; RIGHT: 2 MM IN DIAMETER (FOR >UNREMARKABLE COMPARISON, LEFT IS 1 MM IN DIAMETER) PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SINGLE DARK RED AREA, 3 X 2 MM -B-ADENOMA,-NEOPLASM PRESENT 2380 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 779 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91064 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/05/99 STUDY DAY OF DEATH: 679 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/05/99 14:14 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2381 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 780 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91065 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 719.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 10:32 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- MULTIPLE DARK RED AREAS, UP TO 2 X 1 FOCAL MM; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 6 MM, DARK RED, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL TAN, AND FIRM -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS 2382 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 781 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91065 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 719.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 10:32 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU) ------------------------------------------------------------------------------------------------------------------------------------ 2383 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 782 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91066 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/15/99 STUDY DAY OF DEATH: 598 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 404.3 GRAMS DATE AND TIME OF NECROPSY: 09/15/99 14:43 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE -HEMORRHAGE,-MODERATE, FOCAL >APPEARANCE; HUNCHED; VERIFIED AT -PIGMENT,-SLIGHT, FOCAL NECROPSY: NOT APPLICABLE LIVER (LI) : >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED -HEMORRHAGE,-MINIMAL, FOCAL AT NECROPSY: NOT APPLICABLE -NECROSIS, HEPATOCELLULAR,-MODERATE, >BEHAVIOR; HYPOACTIVE; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >SKIN & PELAGE; COLD TO TOUCH; ENTIRE -CAST, PROTEINACEOUS,-MINIMAL, MULTI- BODY; VERIFIED AT NECROPSY: NOT FOCAL APPLICABLE PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 14 X 10 X 8 MM, RED, DARK -HEMORRHAGE,-MODERATELY SEVERE, MULTI- RED, TAN AND SOFT FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING >UNREMARKABLE URETHRA: GREEN AND FIRM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2384 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 783 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91066 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/15/99 STUDY DAY OF DEATH: 598 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 404.3 GRAMS DATE AND TIME OF NECROPSY: 09/15/99 14:43 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TAIL (TI) : TAIL (TI) : -CRUSTED AREA(S); ENTIRE LENGTH OF -EROSION,-MODERATELY SEVERE, FOCAL TAIL: MULTIPLE TAN, BROWN, AND -INFLAMMATION, ACUTE,-MODERATELY SLIGHTLY RAISED AREAS, UP TO 3 MM IN SEVERE, FOCAL DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2385 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 784 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91066 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/15/99 STUDY DAY OF DEATH: 598 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 404.3 GRAMS DATE AND TIME OF NECROPSY: 09/15/99 14:43 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2386 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 785 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91067 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 696 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/22/99 8:48 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL MUSCLE, SKELETAL (SM) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 12 X 8 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 20 X 10 X 3 >UNREMARKABLE MM 2387 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 786 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91067 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 696 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/22/99 8:48 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2388 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 787 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91068 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/13/98 STUDY DAY OF DEATH: 78 STUDY WEEK OF DEATH: 12 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/13/98 15:01 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -HEMORRHAGE,-MODERATE, FOCAL >APPEARANCE; HUNCHED; VERIFIED AT -HYPERTROPHY, CENTRILOBULAR, NECROPSY: NOT APPLICABLE HEPATOCELLULAR,-SLIGHT, DIFFUSE THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 7 X 1 >UNREMARKABLE MM -GELATINOUS; BILATERAL: CLEAR CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2389 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 788 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91068 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/13/98 STUDY DAY OF DEATH: 78 STUDY WEEK OF DEATH: 12 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/13/98 15:01 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2390 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 789 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91069 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/02/99 STUDY DAY OF DEATH: 585 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/02/99 9:29 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATELY SEVERE, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: -B-ADENOMA,-NEOPLASM PRESENT SINGLE DARK RED FOCUS, 3 MM IN DIAMETER SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT -------------------------------------------- 2391 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 790 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91069 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/02/99 STUDY DAY OF DEATH: 585 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/02/99 9:29 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2392 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 791 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91070 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 584.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 10:43 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : LEFT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -FIBROSIS,-MINIMAL, FOCAL MULTIPLE DARK RED AREAS, UP TO 3 MM IN -PIGMENT,-MINIMAL, FOCAL DIAMETER; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 5 X 5 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- GREY, SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE -EDEMA,-MODERATE, FOCAL DARK BROWN AREA, 1 MM IN DIAMETER -EROSION,-SLIGHT, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD(S) (FP) : -FIBROSIS,-MODERATELY SEVERE, FOCAL 2393 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 792 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91070 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 584.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 10:43 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT HIND -INFLAMMATION, ACUTE,-MODERATE, FOCAL FOOT: SINGLE DARK RED ABRADED AREA 6 X 4 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2394 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 793 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91071 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 913.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:14 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, DIFFUSE PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -FIBROSIS, PLEURAL/SUBPLEURAL,-MINIMAL, MULTIPLE TAN AREAS, UP TO 3 X 2 MM FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, MULTI-FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 5 MM, RED, GREY, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL FIRM -B-ADENOMA,-NEOPLASM PRESENT 2395 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 794 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91071 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 913.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:14 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2396 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 795 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91072 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 475.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:17 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >SKIN & PELAGE; YELLOW HAIR COAT; FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : KIDNEY (KD) : >MASS(ES); MOUTH; WEEK 104; VERIFIED AT -MASS(ES); LEFT, INVOLVING ENTIRE -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: OBSERVED ORGAN: SINGLE MASS, 40 X 40 X 32 MM, MODERATE, MULTI-FOCAL RED, DARK RED, TAN, VASCULAR, AND -M-LIPOSARCOMA,-NEOPLASM PRESENT FIRM; SAME ON CUT SECTION, COLLECTED; REGIONAL LN (RENAL) NOT IDENTIFIED PANCREAS (PA) : PANCREAS (PA) : -LARGE VESSELS; DIFFUSE: UP TO 3 MM IN -HYPERTROPHY, VASCULAR,-MODERATE, MULTI- DIAMETER (CAP 22) FOCAL -INFLAMMATION, VASCULAR,-MINIMAL, MULTI- FOCAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT, FOCAL SKIN (SK) : SKIN (SK) : -MASS(ES); LEFT SIDE OF HEAD, NEAR -INFLAMMATION, CHRONIC,-MODERATELY CORNER OF MOUTH: SINGLE MASS, 8 MM IN SEVERE, FOCAL DIAMETER, DARK RED, AND FIRM; ON -ULCERATION,-MODERATELY SEVERE, FOCAL SECTION EXUDES A LIGHT GREEN SEMISOLID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL; COLLECTED URINARY BLADDER (UB) : -CALCULUS,-PRESENT 2397 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 796 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91072 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 475.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:17 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU) ------------------------------------------------------------------------------------------------------------------------------------ 2398 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 797 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91073 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 963.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:30 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LIVER (LI) : LIVER (LI) : LEFT; VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); LEFT LATERAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >SKIN & PELAGE; YELLOW HAIR COAT; LOBE: MULTIPLE DARK RED AREAS, UP TO 2 FOCAL VERIFIED AT NECROPSY: OBSERVED MM IN DIAMETER, COLLECTED ON ROUTINE >SKIN & PELAGE; BROWN HAIR COAT; SECTION PERINEAL AREA; VERIFIED AT NECROPSY: KIDNEY (KD) : KIDNEY (KD) : OBSERVED -LARGE PELVIS(ES); RIGHT -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); MID ASPECT: TWO -HEMORRHAGE,-MINIMAL, MULTI-FOCAL DARK RED AREAS, EACH IS 2 MM IN -B-ADENOMA,-NEOPLASM PRESENT DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT HIND -EROSION,-MODERATELY SEVERE, FOCAL FOOT: SINGLE DARK RED, MOIST, AND -EXOSTOSES,-MODERATELY SEVERE, FOCAL ABRADED AREA, 15 X 14 MM; COLLECTED -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL 2399 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 798 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91073 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 963.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:30 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY YELLOW AND BROWN MATERIAL -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2400 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 799 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91074 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/06/00 STUDY DAY OF DEATH: 711 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 587.4 GRAMS DATE AND TIME OF NECROPSY: 01/06/00 14:28 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; YELLOW HAIR COAT; -DILATATION, VENTRICULAR,-PRESENT VERIFIED AT NECROPSY: OBSERVED -HEMORRHAGE,-SLIGHT, FOCAL >EXCRETION; NO FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR,-SEVERE, >EYES; SQUINTED; PERI-ORBITAL; VERIFIED DIFFUSE AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : KIDNEY (KD) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -ROUGH SURFACE, DIFFUSE; BILATERAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI- NECROPSY: OBSERVED -DIFFUSELY DARK; BILATERAL: DARK GREEN FOCAL >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED -NEPHROPATHY, CHRONIC PROGRESSIVE,- AT NECROPSY: NOT APPLICABLE MODERATE, MULTI-FOCAL -PYELONEPHRITIS,-MINIMAL, MULTI-FOCAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: BOTH ARE 23 X 11 X -ATROPHY/DEGENERATION,-MODERATE, MULTI- 4 MM, BOTH APPEAR SOFT FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL 2401 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 800 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91074 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/06/00 STUDY DAY OF DEATH: 711 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 587.4 GRAMS DATE AND TIME OF NECROPSY: 01/06/00 14:28 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, RED, DARK -HEMORRHAGE,-SLIGHT, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -EDEMA,-SLIGHT, FOCAL TAN AND BROWN ERODED AREAS, UP TO 5 X -EROSION,-SLIGHT, FOCAL 3 MM -INFLAMMATION, ACUTE,-MINIMAL, MULTI- FOCAL PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,- URETHRA: GREEN AND FIRM MODERATE, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: BOTH ARE 14 X 5 X 2 >UNREMARKABLE MM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HINDFOOT, NEAR -EXOSTOSES,-SLIGHT, FOCAL JUNCTION OF DIGITS 2,3, AND 4: SINGLE -FIBROSIS,-MODERATE, FOCAL TAN AND BROWN, SLIGHTLY RAISED AREA, 5 -INFLAMMATION, ACUTE,-MINIMAL, MULTI- MM IN DIAMETER; COLLECTED FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DARK YELLOW -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED -------------------------------------------- 2402 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 801 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91074 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/06/00 STUDY DAY OF DEATH: 711 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 587.4 GRAMS DATE AND TIME OF NECROPSY: 01/06/00 14:28 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2403 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 802 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91075 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 656.3 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 9:52 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : LEFT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED -NEPHROPATHY, CHRONIC PROGRESSIVE,- AT NECROPSY: NOT APPLICABLE MODERATELY SEVERE, DIFFUSE >BEHAVIOR; HYPOACTIVE; VERIFIED AT PANCREAS (PA) : NECROPSY: NOT APPLICABLE -PIGMENT,-MINIMAL, MULTI-FOCAL >EYES; RED DISCHARGE; EYES; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: OBSERVED -ANGIECTASIS,-SLIGHT, MULTI-FOCAL >RESPIRATION; LABORED; VERIFIED AT -DEGENERATION, CYSTIC, CORTICAL,- NECROPSY: NOT APPLICABLE MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SPINAL CORD (SC) : SPINAL CORD (SC) : >NOTE:>TRIMMING COMMENT: SPINAL CORD >TISSUE MISSING NOT FOUND AT TIME OF TRIMMING LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT 2404 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 803 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91075 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 656.3 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 9:52 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 30 X 15 X 6 -SECRETION, INCREASED,-PRESENT MM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HIND FOOT: -EDEMA,-SLIGHT, MULTI-FOCAL SINGLE TAN, DRY CRUSTED AREA, 6 X 5 -EROSION,-MODERATE, MULTI-FOCAL MM; COLLECTED -INFLAMMATION, ACUTE,-MODERATE, MULTI- -ABRASION(S)/ULCERATION(S); RIGHT HIND FOCAL FOOT: SINGLE DARK RED MOIST ABRADED AREA, 5 X 3 MM; COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; PERIANAL: DARK YELLOW AND MOIST -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED, MOIST MATERIAL -ANIMAL OBESE -------------------------------------------- 2405 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 804 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91075 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 656.3 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 9:52 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2406 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 805 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91076 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 747.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:50 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -HEMORRHAGE,-MINIMAL, MULTI-FOCAL >SKIN & PELAGE; SORE/SCAB; PAW-HIND- MULTIPLE LIGHT GREEN AREAS, UP TO 3 X -INFLAMMATION, ACUTE,-MINIMAL, MULTI- RIGHT; VERIFIED AT NECROPSY: OBSERVED 2 MM; COLLECTED ON ROUTINE SECTIONS FOCAL >SKIN & PELAGE; YELLOW HAIR COAT; -NECROSIS, HEPATOCELLULAR,-MODERATELY VERIFIED AT NECROPSY: OBSERVED SEVERE, MULTI-FOCAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL LUNG (LU) : -MINERALIZATION,-MINIMAL, FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : THYROID (TY) : -LARGE; LEFT: 8 X 4 X 4 MM (RIGHT IS 5 -CYST, ULTIMOBRANCHIAL,-PRESENT X 4 X 2 MM) -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); RIGHT ASPECT: -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SINGLE DARK RED AREA, 2 X 1 MM -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2407 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 806 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91076 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 747.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:50 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS 10 X 4 X 2 -DILATATION, SINUSOIDAL,-MINIMAL, FOCAL MM; COLLECTED -HYPERPLASIA, LYMPHOCYTIC,-MINIMAL, DIFFUSE FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HIND FOOT: -EROSION,-MODERATE, FOCAL SINGLE DRY RED, TAN CRUSTED AREA, 15 X -EXOSTOSES,-MODERATE, FOCAL 10 MM; COLLECTED -INFLAMMATION, ACUTE,-MODERATE, FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS -STAINS - SKIN/HAIRCOAT; ENTIRE ABDOMINAL REGION: DRY YELLOW MATERIAL -BONE MARROW SMEAR TAKEN -------------------------------------------- 2408 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 807 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91076 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 747.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:50 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2409 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 808 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91077 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/05/99 STUDY DAY OF DEATH: 588 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 573.5 GRAMS DATE AND TIME OF NECROPSY: 09/05/99 16:02 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; MISSING; TEETH; VERIFIED -DILATATION, VENTRICULAR,-PRESENT AT NECROPSY: OBSERVED LIVER (LI) : >SKIN & PELAGE; COLD TO TOUCH; ENTIRE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- BODY; VERIFIED AT NECROPSY: NOT FOCAL APPLICABLE KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI- FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL -PYELONEPHRITIS,-MODERATE, MULTI-FOCAL LUNG (LU) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, RED, DARK -HEMORRHAGE,-SLIGHT, MULTI-FOCAL RED, TAN AND FIRM -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL -EDEMA,-MODERATE, MULTI-FOCAL 2410 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 809 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91077 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/05/99 STUDY DAY OF DEATH: 588 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 573.5 GRAMS DATE AND TIME OF NECROPSY: 09/05/99 16:02 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 35 X 20 X 4 -INFLAMMATION, ACUTE,-MODERATELY MM SEVERE, FOCAL -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS 9 X 5 X 3 -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MM DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS -TEETH MISSING/BROKEN/WORN; MISSING, BILATERAL UPPER INCISORS -------------------------------------------- 2411 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 810 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91077 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/05/99 STUDY DAY OF DEATH: 588 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 573.5 GRAMS DATE AND TIME OF NECROPSY: 09/05/99 16:02 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2412 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 811 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91078 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/05/99 STUDY DAY OF DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/05/99 7:43 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : LIVER (LI) : -LARGE; ALL LOBES -DEGENERATION, CYSTIC,-MINIMAL, FOCAL -RED FOCUS(I)/AREA(S); ALL LOBES: -HYPERPLASIA, BILE DUCT,-MODERATE, MULTIPLE DARK RED AREAS, UP TO 3 X 2 MULTI-FOCAL MM; COLLECTED ON ROUTINE SECTIONS -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -STASIS, BILE,-MODERATE, MULTI-FOCAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT -LARGE; BILATERAL: EACH IS 35 X 25 X -NEPHROPATHY, CHRONIC PROGRESSIVE,- 19 MM MODERATELY SEVERE, DIFFUSE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL, MULTI-FOCAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 18 X 7 X 5 >UNREMARKABLE MM LUNG (LU) : LUNG (LU) : -UNCOLLAPSED; ALL LOBES -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED FOCI, UP TO 2 MM IN DIAMETER 2413 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 812 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91078 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/05/99 STUDY DAY OF DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/05/99 7:43 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL THYROID (TY) : THYROID (TY) : -LARGE; BILATERAL: EACH IS 9 X 5 X 4 -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM MM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 9 X 8 MM, RED, DARK -HEMORRHAGE,-MODERATELY SEVERE, MULTI- RED, GREY, TAN, LOBULATED AND SOFT FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 3 X 3 >UNREMARKABLE MM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT URETER (UE) : URETER (UE) : -LARGE; BILATERAL: EACH IS UP TO 1 MM >TISSUE MISSING IN DIAMETER (CAP 22) GENERAL COMMENT (GC) : -GI TRACT-LARGE LUMEN; INVOLVING ENTIRE GI TRACT: UP TO 35 MM IN DIAMETER 2414 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 813 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91078 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/05/99 STUDY DAY OF DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/05/99 7:43 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2415 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 814 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91079 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 449.6 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 13:09 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >RESPIRATION; IRREGULAR; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >APPEARANCE; HEAD TILT; RIGHT; VERIFIED KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >BEHAVIOR; ATAXIC; VERIFIED AT SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE TESTIS (TE) : -MINERALIZATION, VASCULAR,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATELY SEVERE, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 15 X 7 X 7 MM, -B-ADENOMA,-NEOPLASM PRESENT RED, DARK RED, TAN, AND SOFT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, ACUTE,-SLIGHT, MULTI- URETHRA: GREEN AND FIRM FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; LEFT: 32 X 18 X 3 MM (RIGHT IS -SECRETION, INCREASED,-PRESENT 26 X 12 X 3 MM) 2416 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 815 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91079 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 449.6 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 13:09 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2417 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 816 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91080 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/07/99 STUDY DAY OF DEATH: 681 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/07/99 11:18 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); EAR-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 65; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >WART LIKE LESION(S); VENTRAL- -DILATATION, VENTRICULAR,-PRESENT ABDOMINAL; WEEK 76; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -DEGENERATION, CYSTIC,-SLIGHT, FOCAL >SKIN & PELAGE; SORE/SCAB; PAW-HIND- KIDNEY (KD) : LEFT; VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >APPEARANCE; THIN; VERIFIED AT FOCAL NECROPSY: NOT OBSERVED -MINERALIZATION, PELVIC,-SLIGHT, FOCAL >EXCRETION; FEW FECES; VERIFIED AT HEART (HT) : NECROPSY: NOT APPLICABLE -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, DARK RED AND -HEMORRHAGE,-MODERATELY SEVERE, MULTI- SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : SKIN (SK) : -MASS(ES); MID VENTRAL THORACIC -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM REGION: SINGLE MASS, 15 X 10 X 6 MM, PRESENT TAN AND HARD; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (AXILLARY) NORMAL (CAP 24) SEMINAL VESICLES (SV) : -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL 2418 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 817 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91080 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/07/99 STUDY DAY OF DEATH: 681 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/07/99 11:18 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: -EROSION,-MODERATELY SEVERE, FOCAL SINGLE DRY BROWN CRUSTED AREA, 15 MM -FIBROSIS,-SLIGHT, FOCAL IN DIAMETER; SURROUNDING TISSUE -INFLAMMATION, ACUTE,-MODERATE, FOCAL APPEARS SWOLLEN; COLLECTED PINNA (PN) : PINNA (PN) : -CRUSTED AREA(S); LEFT, NEAR CANAL: >UNREMARKABLE SINGLE DRY BROWN AREA, 3 X 2 X 1 MM (CAP 22) -------------------------------------------- 2419 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 818 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91080 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/07/99 STUDY DAY OF DEATH: 681 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/07/99 11:18 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2420 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 819 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91099 SEX: MALE DOSE GROUP: 7 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/05/98 STUDY DAY OF DEATH: 39 STUDY WEEK OF DEATH: 6 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/05/98 7:57 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -HYPERTROPHY, CENTRILOBULAR, >DISCHARGE; ORAL; RED IN COLOR; HEPATOCELLULAR,-MODERATELY SEVERE, VERIFIED AT NECROPSY: OBSERVED DIFFUSE >BEHAVIOR; HYPOACTIVE; VERIFIED AT -NECROSIS, HEPATOCELLULAR,-MODERATE, NECROPSY: NOT APPLICABLE MULTI-FOCAL >SKIN & PELAGE; YELLOW HAIR COAT; THYROID (TY) : VERIFIED AT NECROPSY: OBSERVED -ECTOPIC THYMUS,-PRESENT >HOUSING,FOOD,WATER & MAINT.; WATER STOMACH, GL (ST) : STOMACH, GL (ST) : BOTTLE PLACED ON CAGE; VERIFIED AT -DARK FOCUS(I)/AREA(S); MUCOSA: -EROSION,-MODERATE, FOCAL NECROPSY: NOT APPLICABLE MULTIPLE DARK BROWN AREAS, PINPOINT >APPEARANCE; DEHYDRATED; VERIFIED AT AND UP TO 3 X 1 MM NECROPSY: NOT APPLICABLE JEJUNUM (JE) : >AUTOLYTIC CAUSE OF DEATH (CD) : -HEPATIC NECROSIS,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-ORAL; RED, DRY MATERIAL -STAINS-PERINEUM/PERIANAL; YELLOW MOIST MATERIAL -------------------------------------------- 2421 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 820 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91099 SEX: MALE DOSE GROUP: 7 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/05/98 STUDY DAY OF DEATH: 39 STUDY WEEK OF DEATH: 6 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/05/98 7:57 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2422 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 821 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91121 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 397.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:02 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0466 .0117 % .0223 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0388 .0098 % .0186 WEIGHT TAKEN BRAIN (BR) 2.0851 .5242 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.3222 .3324 % .6341 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.5011 .3774 % .7199 WEIGHT TAKEN LIVER (LI) 11.8627 2.9821 % 5.6893 WEIGHT TAKEN LUNG (LU) 1.9458 .4891 % .9332 WEIGHT TAKEN LEFT OVARY (OV0) .0548 .0138 % .0263 WEIGHT TAKEN RIGHT OVARY (OV1) .0515 .0129 % .0247 WEIGHT TAKEN SPLEEN (SP) .5875 .1477 % .2818 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0211 .0053 % .0101 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0171 .0043 % .0082 WEIGHT TAKEN UTERUS (UT) 1.7745 .4461 % .8510 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK BROWN AREAS, UP TO 1 MM IN DIAMETER 2423 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 822 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91121 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 397.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:02 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: -GALACTOCELE,-SLIGHT, MULTI-FOCAL MULTIPLE TAN AND CREAMY SEMIFLUID -B-FIBROADENOMA,-NEOPLASM PRESENT FILLED CYSTS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: -DILATATION,-MODERATE, DIFFUSE CLEAR FLUID CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2424 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 823 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91121 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 397.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:02 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2425 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 824 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91122 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 451.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:33 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0420 .0093 % .0185 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0248 .0055 % .0109 WEIGHT TAKEN BRAIN (BR) 2.2764 .5046 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.3642 .3024 % .5993 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.3425 .2976 % .5897 WEIGHT TAKEN LIVER (LI) 10.7372 2.3802 % 4.7167 WEIGHT TAKEN LUNG (LU) 1.5978 .3542 % .7019 WEIGHT TAKEN LEFT OVARY (OV0) .0558 .0124 % .0245 WEIGHT TAKEN RIGHT OVARY (OV1) .0565 .0125 % .0248 WEIGHT TAKEN SPLEEN (SP) .6826 .1513 % .2999 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0168 .0037 % .0074 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0214 .0047 % .0094 WEIGHT TAKEN UTERUS (UT) .4623 .1025 % .2031 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, FOCAL 2426 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 825 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91122 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 451.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:33 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2427 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 826 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91122 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 451.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:33 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2428 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 827 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91123 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/09/99 STUDY DAY OF DEATH: 683 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/09/99 14:00 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 85; 50 X 60MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); INGUINAL REGION-RIGHT; WEEK -HEMORRHAGE,-MODERATE, MULTI-FOCAL 94; 40 X 40MM; VERIFIED AT NECROPSY: LIVER (LI) : LIVER (LI) : OBSERVED -LARGE; ALL LOBES; MARGINS APPEAR >UNREMARKABLE >SKIN & PELAGE; SORE/SCAB; DORSAL; ROUNDED VERIFIED AT NECROPSY: NOT OBSERVED KIDNEY (KD) : >MASS(ES); AXILLARY REGION-LEFT; WEEK -CAST, PROTEINACEOUS,-SLIGHT, MULTI- 97; VERIFIED AT NECROPSY: OBSERVED FOCAL >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED -MINERALIZATION, PELVIC,-SLIGHT, MULTI- AT NECROPSY: NOT APPLICABLE FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : NECROPSY: NOT APPLICABLE -LARGE; BILATERAL: LEFT IS 8 X 8 X 6 -ANGIECTASIS,-SEVERE, MULTI-FOCAL >RESPIRATION; LABORED; VERIFIED AT MM, RIGHT IS 10 X 8 X 8 MM -DEGENERATION, CYSTIC, CORTICAL,- NECROPSY: NOT APPLICABLE -DIFFUSELY DARK; BILATERAL: DARK BROWN SEVERE, MULTI-FOCAL -HEMORRHAGE,-SEVERE, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : THYMUS (TH) : -MOTTLED; RED, DARK RED, GRAY AND TAN -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -LARGE; 35 X 25 X 6 MM -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2429 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 828 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91123 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/09/99 STUDY DAY OF DEATH: 683 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/09/99 14:00 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 4 MM, DARK RED, -HEMORRHAGE,-SEVERE, MULTI-FOCAL TAN, SOFT AND LOBULATED -B-ADENOMA,-NEOPLASM PRESENT ILEUM (IL) : >AUTOLYTIC COLON (CO) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL SINGLE MASS, 65 X 55 X 40 MM, TAN, -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT RED, SOFT AND VASCULAR; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) -CYST(S); VENTRAL ABDOMINAL REGION: MULTIPLE TAN, CREAMY, SEMIFLUID FILLED CYSTS, UP TO 12 X 10 X 5 MM; COLLECTED ON ROUTINE SECTION -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 15 X 13 X 6 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND SOFT; COLLECTED ON ROUTINE SECTION 2430 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 829 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91123 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/09/99 STUDY DAY OF DEATH: 683 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/09/99 14:00 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #3; RIGHT INGUINAL REGION: SINGLE MASS, 50 X 40 X 25 MM, TAN AND SOFT; CUT SURFACE EXUDES A TAN CREAMY SEMIFLUID MATERIAL; COLLECTED INTACT; REGIONAL LN (RIGHT INGUINAL) NORMAL (CAP 22) CAUSE OF DEATH (CD) : -MALIGNANT LYMPHOMA,-PRESENT HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: TAN AND BROWN >UNREMARKABLE (CAP 21) LN, INGUINAL (IN) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -------------------------------------------- 2431 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 830 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91123 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/09/99 STUDY DAY OF DEATH: 683 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/09/99 14:00 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA) ------------------------------------------------------------------------------------------------------------------------------------ 2432 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 831 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91124 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 363.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:53 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0661 .0182 % .0303 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0603 .0166 % .0277 WEIGHT TAKEN BRAIN (BR) 2.1807 .6004 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.5783 .4346 % .7238 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.6324 .4494 % .7486 WEIGHT TAKEN LIVER (LI) 11.4796 3.1607 % 5.2642 WEIGHT TAKEN LUNG (LU) 1.6056 .4421 % .7363 WEIGHT TAKEN LEFT OVARY (OV0) .0650 .0179 % .0298 WEIGHT TAKEN RIGHT OVARY (OV1) .0626 .0172 % .0287 WEIGHT TAKEN SPLEEN (SP) .6360 .1751 % .2916 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0129 .0036 % .0059 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0097 .0027 % .0044 WEIGHT TAKEN UTERUS (UT) 1.2016 .3308 % .5510 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-SLIGHT, MULTI- FOCAL ADRENAL, CORTEX (AC) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); RIGHT ASPECT: -B-ADENOMA,-NEOPLASM PRESENT TWO BROWN FOCI, BOTH ARE 1 MM IN DIAMETER STOMACH, GL (ST) : -EDEMA,-SLIGHT, FOCAL 2433 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 832 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91124 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 363.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:53 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2434 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 833 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91124 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 363.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:53 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2435 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 834 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91125 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/27/99 STUDY DAY OF DEATH: 671 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 353.9 GRAMS DATE AND TIME OF NECROPSY: 11/27/99 13:46 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-RIGHT; WEEK LIVER (LI) : LIVER (LI) : 87; 70 X 55MM; VERIFIED AT NECROPSY: -DIFFUSELY LIGHT; ALL LOBES: TAN -VACUOLATION, HEPATOCELLULAR,-MODERATE, OBSERVED MULTI-FOCAL >EXCRETION; NO FECES; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MODERATE, >EYES; RED DISCHARGE; EYES; VERIFIED AT MULTI-FOCAL NECROPSY: OBSERVED -MINERALIZATION, TUBULAR,-MINIMAL, >RESPIRATION; LABORED; VERIFIED AT MULTI-FOCAL NECROPSY: NOT APPLICABLE ADRENAL, CORTEX (AC) : >APPEARANCE; THIN; VERIFIED AT -ANGIECTASIS,-SLIGHT, MULTI-FOCAL NECROPSY: NOT OBSERVED -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -HYPERTROPHY, CORTICAL CELL,-MODERATE, FOCAL LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY RED; DARK RED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2436 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 835 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91125 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/27/99 STUDY DAY OF DEATH: 671 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 353.9 GRAMS DATE AND TIME OF NECROPSY: 11/27/99 13:46 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -MASS(ES); RIGHT POSTERIOR ASPECT: SINGLE MASS, 5 X 4 X 2 MM, DARK RED AND SOFT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -RAISED AREA(S); MUCOSA: SINGLE WHITE -FIBROSIS,-MODERATELY SEVERE, FOCAL AREA, 7 X 5 X 3 MM -HYPERKERATOSIS, NONGLANDULAR MUCOSA,- MODERATE, DIFFUSE -INFLAMMATION, ACUTE,-MODERATE, FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT PERINEAL REGION: -GALACTOCELE,-MODERATELY SEVERE, MULTI- SINGLE MASS, 65 X 65 X 25 MM, TAN, FOCAL DARK RED, RED, LOBULATED AND SOFT; -HEMORRHAGE,-MODERATE, MULTI-FOCAL SAME ON CUT SURFACE; COLLECTED; -B-FIBROADENOMA,-NEOPLASM PRESENT REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -MAMMARY FIBROADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -------------------------------------------- 2437 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 836 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91125 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/27/99 STUDY DAY OF DEATH: 671 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 353.9 GRAMS DATE AND TIME OF NECROPSY: 11/27/99 13:46 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2438 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 837 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91126 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 229.2 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 7:41 PROSECTOR: WENDY CHERRY RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0378 .0165 % .0192 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0353 .0154 % .0180 WEIGHT TAKEN BRAIN (BR) 1.9637 .8568 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) .9111 .3975 % .4640 WEIGHT TAKEN RIGHT KIDNEY (KD1) .9189 .4009 % .4679 WEIGHT TAKEN LIVER (LI) 6.8127 2.9724 % 3.4693 WEIGHT TAKEN LUNG (LU) 1.1246 .4907 % .5727 WEIGHT TAKEN LEFT OVARY (OV0) .0507 .0221 % .0258 WEIGHT TAKEN RIGHT OVARY (OV1) .0498 .0217 % .0254 WEIGHT TAKEN SPLEEN (SP) .4642 .2025 % .2364 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0080 .0035 % .0041 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0070 .0031 % .0036 WEIGHT TAKEN UTERUS (UT) .3980 .1736 % .2027 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MINIMAL, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN 2439 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 838 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91126 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 229.2 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 7:41 PROSECTOR: WENDY CHERRY RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL INFORMATION (XX) : >NOTE:>ANIMAL DIED PRIOR TO EXSANGUINATION ANIMAL NECROPSY PERFORMED OUT OF ORDER DUE TO MORIBUND CONDITION -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2440 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 839 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91127 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 353.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:03 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; LATERAL- EYE (EY) : ABDOMINAL-LEFT; VERIFIED AT NECROPSY: -INFLAMMATION, ACUTE,-MINIMAL, FOCAL NOT OBSERVED -ULCERATION, CORNEAL,-MINIMAL, FOCAL >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; BRAIN (BR) : BRAIN (BR) : WEEK 91; 60 X 50MM; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -RED FOCUS(I)/AREA(S); ALL LOBES: -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTIPLE DARK RED AREAS, UP TO 2 X 1 MULTI-FOCAL MM; COLLECTED ON ROUTINE SECTIONS LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT, MULTI- FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT 2441 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 840 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91127 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 353.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:03 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -MASS(ES); LEFT: SINGLE MASS, 15 X 11 -DEGENERATION, CYSTIC, CORTICAL,- X 11 MM, RED, DARK RED, TAN AND FIRM; SEVERE, FOCAL SAME ON CUT SURFACE; COLLECTED; -HEMORRHAGE,-SEVERE, FOCAL REGIONAL LN (SEE RENAL) LN, MESENTERIC (MS) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LUNG (LU) : -FIBROSIS, PLEURAL/SUBPLEURAL,-MINIMAL, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 11 X 8 X 6 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, DARK RED, GRAY, LOBULATED AND -B-ADENOMA,-NEOPLASM PRESENT SOFT CECUM (CE) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); LEFT INGUINAL -INFLAMMATION, CHRONIC-ACTIVE,- REGION: SINGLE DRY TAN AND BROWN MODERATE, FOCAL CRUSTED AREA, 25 X 10 MM (CAP 20) MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 70 X 50 X 30 MM, RED, -B-ADENOMA,-NEOPLASM PRESENT DARK RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED VAGINA (VA) : >UNREMARKABLE 2442 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 841 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91127 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 353.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:03 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: CLEAR MUCOID MATERIAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; LEFT: 9 X 5 X 5 MM (CAP 21) -HEMORRHAGE,-MODERATE, MULTI-FOCAL -DIFFUSELY RED; RIGHT: DARK RED -SMALL; RIGHT: 2 X 1 X 1 MM (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL NECROPSIED OUT OF ORDER DUE TO MORIBUND CONDITION -------------------------------------------- 2443 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 842 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91127 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 353.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:03 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2444 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 843 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91128 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/02/99 STUDY DAY OF DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 554.6 GRAMS DATE AND TIME OF NECROPSY: 07/02/99 9:58 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; VENTRAL- BRAIN (BR) : BRAIN (BR) : ABDOMINAL; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; RED HAIR COAT; HEAD- KIDNEY (KD) : ENTIRE; VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >EXCRETION; FEW FECES; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, DARK RED AND -HEMORRHAGE,-MODERATE, MULTI-FOCAL SOFT -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; DIFFUSE: TAN AND FIRM; -GALACTOCELE,-MINIMAL, MULTI-FOCAL COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2445 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 844 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91128 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/02/99 STUDY DAY OF DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 554.6 GRAMS DATE AND TIME OF NECROPSY: 07/02/99 9:58 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD AND VENTRAL ABDOMINAL REGION: DARK RED AND DRY MATERIAL -------------------------------------------- 2446 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 845 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91128 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/02/99 STUDY DAY OF DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 554.6 GRAMS DATE AND TIME OF NECROPSY: 07/02/99 9:58 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2447 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 846 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91129 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 367.4 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:08 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0283 .0077 % .0138 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0247 .0067 % .0120 WEIGHT TAKEN BRAIN (BR) 2.0540 .5591 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.3979 .3805 % .6806 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.4025 .3817 % .6828 WEIGHT TAKEN LIVER (LI) 10.5662 2.8759 % 5.1442 WEIGHT TAKEN LUNG (LU) 1.2784 .3480 % .6224 WEIGHT TAKEN LEFT OVARY (OV0) .0560 .0152 % .0273 WEIGHT TAKEN RIGHT OVARY (OV1) .0486 .0132 % .0237 WEIGHT TAKEN SPLEEN (SP) .5479 .1491 % .2667 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0130 .0035 % .0063 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0045 .0012 % .0022 WEIGHT TAKEN UTERUS (UT) .6525 .1776 % .3177 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2448 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 847 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91129 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 367.4 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:08 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2449 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 848 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91130 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/13/99 STUDY DAY OF DEATH: 534 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/13/99 14:44 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); RIGHT MEDIAN >UNREMARKABLE LOBE: SINGLE DARK RED AREA, 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL NERVE, SCIATIC (SN) : >TISSUE MISSING PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC CERVIX (CV) : CERVIX (CV) : -LARGE; 8 X 6 X 5 MM >UNREMARKABLE 2450 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 849 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91130 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/13/99 STUDY DAY OF DEATH: 534 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/13/99 14:44 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND MOIST -DISCHARGE-NASAL; DARK RED AND DRY -------------------------------------------- 2451 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 850 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91130 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/13/99 STUDY DAY OF DEATH: 534 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/13/99 14:44 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2452 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 851 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91131 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 341.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:43 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT LIVER, OIL RED O (LI1) : NECROPSY: NOT OBSERVED -POSITIVE OIL RED O STAINING,-MINIMAL, >SKIN & PELAGE; SORE/SCAB; PAW-HIND- MULTI-FOCAL LEFT; VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL -PYELONEPHRITIS,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 8 MM IN DIAMETER (LEFT -DEGENERATION, CYSTIC, CORTICAL,- IS 4 X 4 X 3 MM) MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID >UNREMARKABLE FILLED CYST, 7 MM IN DIAMETER LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 5 X 4 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL UTERUS (UT) : -DILATATION,-MODERATELY SEVERE, DIFFUSE 2453 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 852 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91131 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 341.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:43 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : UTERUS (UT) : -LARGE, DIFFUSE; BILATERAL: LEFT HORN -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- IS 70 X UP TO 15 X 3 MM, RIGHT HORN IS SLIGHT, MULTI-FOCAL 70 X 10 X 3 MM -HYPERPLASIA, ENDOMETRIAL,-MODERATE, DIFFUSE -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL: RED AND TAN (CAP -DILATATION, SINUSOIDAL,-MINIMAL, MULTI- 20) FOCAL -HYPERPLASIA, LYMPHOCYTIC,-MINIMAL, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: -EROSION,-MODERATE, FOCAL SINGLE DARK RED AND DRY AREA, 5 X 3 -FIBROSIS,-MODERATE, FOCAL MM; COLLECTED -INFLAMMATION, ACUTE,-MODERATE, FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2454 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 853 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91131 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 341.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:43 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2455 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 854 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91132 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 438.1 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 14:34 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 64; 80 X 90MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED KIDNEY (KD) : >SKIN & PELAGE; BLUE SKIN; VENTRAL- -MINERALIZATION, PELVIC,-MINIMAL, MULTI- ABDOMINAL-LEFT; VERIFIED AT NECROPSY: FOCAL NOT APPLICABLE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 4 MM IN DIAMETER THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 5 X 5 X 3 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, DARK RED, TAN, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : SKIN (SK) : >NOTE:>ROUTINE SECTION COLLECTED FROM -INFLAMMATION, ACUTE,-MODERATELY RIGHT INGUINAL REGION DUE TO SEVERE, FOCAL LARGE MASS -ULCERATION,-SEVERE, FOCAL MAMMARY, FEMALE (MF) : -MINERALIZATION,-MODERATE, MULTI-FOCAL 2456 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 855 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91132 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 438.1 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 14:34 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -PIGMENT,-MODERATELY SEVERE, MULTI- SINGLE MASS, 105 X 64 X 36 MM, TAN, FOCAL RED, DARK RED, BROWN, LOBULATED, AND -B-FIBROADENOMA,-NEOPLASM PRESENT CYSTIC; ON SECTION EXUDES A DARK BROWN -M-CARCINOMA,-NEOPLASM PRESENT FLUID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 21) -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 28 X 15 X 13 MM, DARK RED, TAN, AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY) >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGION DUE TO LARGE MASS UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN LUMEN: -DILATATION,-MODERATELY SEVERE, DIFFUSE MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER CAUSE OF DEATH (CD) : -MAMMARY FIBROADENOMA,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION LN, AXILLARY (AX) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 2457 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 856 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91132 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 438.1 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 14:34 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 13 X 7 X 2 MM (RIGHT IS UP TO 3 X 2 X 2 MM) -DIFFUSELY RED; BILATERAL: DARK RED (CAP 22) LN, INGUINAL (IN) : >TISSUE MISSING GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2458 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 857 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91132 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 438.1 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 14:34 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2459 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 858 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91133 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 420.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:33 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0276 .0066 % .0125 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0300 .0071 % .0136 WEIGHT TAKEN BRAIN (BR) 2.2072 .5253 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.1849 .2820 % .5368 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.2369 .2944 % .5604 WEIGHT TAKEN LIVER (LI) 10.3579 2.4650 % 4.6928 WEIGHT TAKEN LUNG (LU) 1.7630 .4196 % .7987 WEIGHT TAKEN LEFT OVARY (OV0) .0847 .0202 % .0384 WEIGHT TAKEN RIGHT OVARY (OV1) .0766 .0182 % .0347 WEIGHT TAKEN SPLEEN (SP) .5825 .1386 % .2639 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0090 .0021 % .0041 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0192 .0046 % .0087 WEIGHT TAKEN UTERUS (UT) .8435 .2007 % .3822 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : >NOTE:>TRIM COMMENT: MISSING ONE -ONE EXAMINED,-PRESENT ADRENAL AT TIME OF TRIMMING >NOTE:>ONE EXAMINED ADRENAL, MEDULLA (MA) : -ONE EXAMINED,-PRESENT >NOTE:>ONE EXAMINED CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2460 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 859 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91133 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 420.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:33 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2461 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 860 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91133 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 420.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:33 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2462 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 861 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91134 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 256.2 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:24 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL; WEEK 51; BRAIN (BR) : BRAIN (BR) : 60 X 40MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE -NECROSIS, HEPATOCELLULAR,-MODERATE, >APPEARANCE; THIN; VERIFIED AT MULTI-FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >SKIN & PELAGE; RED HAIR COAT; LIMBS- -CAST, PROTEINACEOUS,-SLIGHT, MULTI- FRONT; VERIFIED AT NECROPSY: NOT FOCAL OBSERVED ADRENAL, CORTEX (AC) : >APPEARANCE; HUNCHED; VERIFIED AT -DEGENERATION, CYSTIC, CORTICAL,- NECROPSY: NOT APPLICABLE MODERATE, FOCAL >SKIN & PELAGE; COLD TO TOUCH; ENTIRE -HEMORRHAGE,-SLIGHT, FOCAL BODY; VERIFIED AT NECROPSY: NOT PITUITARY (PI) : PITUITARY (PI) : APPLICABLE -MASS(ES); SINGLE MASS, 12 X 10 X 8 -HEMORRHAGE,-MODERATELY SEVERE, MULTI- >EYES; RED DISCHARGE; EYES; VERIFIED AT MM, DARK RED AND SOFT FOCAL NECROPSY: NOT OBSERVED -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL SINGLE MASS, 60 X 50 X 25 MM, RED, -MINERALIZATION,-MODERATE, MULTI-FOCAL DARK RED, TAN, FIRM AND LOBULATED; -M-CARCINOMA,-NEOPLASM PRESENT SAME ON CUT SURFACE; SOME AREAS EXUDE A TAN AND CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED 2463 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 862 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91134 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 256.2 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:24 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -CYST(S); RIGHT PERIANAL REGION: SINGLE CYST, 13 MM IN DIAMETER, FILLED WITH A TAN CREAMY SEMIFLUID CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2464 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 863 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91134 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 256.2 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:24 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2465 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 864 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91135 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 192.4 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:13 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 6.4555 3.3552 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2466 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 865 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91135 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 192.4 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:13 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2467 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 866 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91136 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/11/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 417.0 GRAMS DATE AND TIME OF NECROPSY: 10/11/99 12:14 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EYES; RED DISCHARGE; EYE-RIGHT; -DILATATION, VENTRICULAR,-PRESENT VERIFIED AT NECROPSY: OBSERVED -HEMORRHAGE,-SLIGHT, FOCAL >APPEARANCE; HEAD TILT; RIGHT; VERIFIED KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MODERATE, >SKIN & PELAGE; YELLOW HAIR COAT; MULTI-FOCAL VERIFIED AT NECROPSY: NOT OBSERVED ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 8 X 7 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL DARK RED, GREY, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -DILATATION,-SEVERE, DIFFUSE MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 25 X 8 X 8 MM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2468 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 867 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91136 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/11/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 417.0 GRAMS DATE AND TIME OF NECROPSY: 10/11/99 12:14 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: -EROSION,-MODERATE, FOCAL SINGLE DARK RED AND DRY AREA, 7 X 5 -FIBROSIS,-MODERATE, FOCAL MM; COLLECTED -INFLAMMATION, ACUTE,-MODERATE, FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED MOIST MATERIAL GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY -------------------------------------------- 2469 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 868 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91136 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/11/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 417.0 GRAMS DATE AND TIME OF NECROPSY: 10/11/99 12:14 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2470 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 869 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91137 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 354.1 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:23 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; PAW-HIND-RIGHT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; LIMITED USE; PAW-HIND- -DILATATION, VENTRICULAR,-PRESENT RIGHT; VERIFIED AT NECROPSY: NOT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL APPLICABLE LIVER (LI) : LIVER (LI) : >EXCRETION; NO FECES; VERIFIED AT -DIFFUSELY LIGHT; ALL LOBES: TAN -VACUOLATION, HEPATOCELLULAR,-SEVERE, NECROPSY: NOT APPLICABLE DIFFUSE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >SKIN & PELAGE; RED HAIR COAT; HEAD- FOCAL ENTIRE; VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-SLIGHT, FOCAL >SKIN & PELAGE; BROWN HAIR COAT; ADRENAL, CORTEX (AC) : PERINEAL AREA; VERIFIED AT NECROPSY: -ANGIECTASIS,-MINIMAL, MULTI-FOCAL OBSERVED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -HYPERTROPHY, CORTICAL CELL,-MODERATE, NECROPSY: NOT APPLICABLE FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 MM IN DIAMETER, DARK RED -HEMORRHAGE,-SEVERE, MULTI-FOCAL AND SOFT -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: -EDEMA,-SLIGHT, FOCAL MULTIPLE TAN INDISTINCT AREAS -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL 2471 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 870 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91137 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 354.1 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:23 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, FOCAL CERVIX (CV) : -DILATATION,-SLIGHT, FOCAL -B-POLYP, ENDOMETRIAL STROMAL,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; RIGHT HINDFOOT: UP TO 12 MM IN -EXOSTOSES,-SEVERE, MULTI-FOCAL DIAMETER; COLLECTED -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -DISCHARGE-NASAL; DRY RED MATERIAL -STAINS - SKIN/HAIRCOAT; HEAD: DRY RED MATERIAL -------------------------------------------- 2472 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 871 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91137 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 354.1 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:23 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2473 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 872 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91138 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 418.8 GRAMS DATE AND TIME OF NECROPSY: 10/27/00 10:06 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); AXILLARY REGION-LEFT; WEEK -DILATATION, VENTRICULAR,-PRESENT 99; 50 X 70MM; VERIFIED AT NECROPSY: KIDNEY (KD) : OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 5 X 4 MM, DARK RED, -HEMORRHAGE,-SEVERE, MULTI-FOCAL TAN, AND FIRM -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -MINERALIZATION,-SLIGHT, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT 2474 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 873 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91138 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 418.8 GRAMS DATE AND TIME OF NECROPSY: 10/27/00 10:06 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 90 X 60 X 30 MM, TAN, VASCULAR, AND SOFT; ON SECTION EXUDES A TAN CREAMY SEMIFLUID; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) CERVIX (CV) : -PIGMENT,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2475 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 874 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91138 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 418.8 GRAMS DATE AND TIME OF NECROPSY: 10/27/00 10:06 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2476 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 875 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91139 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/02/99 STUDY DAY OF DEATH: 432 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/02/99 10:02 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYE-RIGHT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; HEAD TILT; RIGHT; VERIFIED -DILATATION, VENTRICULAR,-PRESENT AT NECROPSY: NOT APPLICABLE LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 6 X 11 MM, -B-ADENOMA,-NEOPLASM PRESENT FIRM, RED, DARK RED AND TAN HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DRY RED MATERIAL -------------------------------------------- 2477 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 876 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91139 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/02/99 STUDY DAY OF DEATH: 432 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/02/99 10:02 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2478 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 877 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91140 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 301.6 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:05 PROSECTOR: TODD ACKER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0531 .0176 % .0258 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0479 .0159 % .0233 WEIGHT TAKEN BRAIN (BR) 2.0571 .6821 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.0500 .3481 % .5104 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.0632 .3525 % .5168 WEIGHT TAKEN LIVER (LI) 8.5377 2.8308 % 4.1504 WEIGHT TAKEN LUNG (LU) 1.4318 .4747 % .6960 WEIGHT TAKEN LEFT OVARY (OV0) .0763 .0253 % .0371 WEIGHT TAKEN RIGHT OVARY (OV1) .0930 .0308 % .0452 WEIGHT TAKEN SPLEEN (SP) .4953 .1642 % .2408 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0103 .0034 % .0050 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0099 .0033 % .0048 WEIGHT TAKEN UTERUS (UT) .5868 .1946 % .2853 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2479 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 878 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91140 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 301.6 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:05 PROSECTOR: TODD ACKER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2480 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 879 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91141 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/06/99 STUDY DAY OF DEATH: 558 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/06/99 7:24 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); LIMB-HIND-RIGHT; WEEK 71; 20 BRAIN (BR) : BRAIN (BR) : X 30MM; VERIFIED AT NECROPSY: NOT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CYST,-MODERATE, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EDEMA,-MODERATE, MULTI-FOCAL MULTIPLE BROWN AND DARK BROWN AREAS, -HEMORRHAGE,-MINIMAL, MULTI-FOCAL UP TO 4 X 3 MM -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL 2481 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 880 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91141 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/06/99 STUDY DAY OF DEATH: 558 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/06/99 7:24 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 40 X 40 X 20 MM, RED, DARK RED, GRAY, TAN, LOBULATED, AND FIRM; ON SECTION, EXUDES A BROWN FLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED CERVIX (CV) : CERVIX (CV) : -LARGE; 10 X 9 X 8 MM >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY MATERIAL -------------------------------------------- 2482 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 881 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91141 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/06/99 STUDY DAY OF DEATH: 558 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/06/99 7:24 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2483 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 882 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91142 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/14/99 STUDY DAY OF DEATH: 505 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 364.2 GRAMS DATE AND TIME OF NECROPSY: 06/14/99 14:48 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; NO FECES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >MASS(ES); INGUINAL REGION-RIGHT; WEEK -MOTTLED; ALL LOBES: RED AND TAN -CYST,-SLIGHT, FOCAL 72; 15 X 15MM; VERIFIED AT NECROPSY: -RED FOCUS(I)/AREA(S); ALL LOBES: -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- OBSERVED MULTIPLE DARK RED AREAS, UP TO 3 X 2 FOCAL >SKIN & PELAGE; RED HAIR COAT; VENTRAL- MM; COLLECTED ON ROUTINE SECTIONS -VACUOLATION, HEPATOCELLULAR,- ABDOMINAL; VERIFIED AT NECROPSY: MODERATELY SEVERE, MULTI-FOCAL OBSERVED KIDNEY (KD) : KIDNEY (KD) : >SKIN & PELAGE; YELLOW HAIR COAT; -LARGE PELVIS(ES); BILATERAL -CYST,-MINIMAL, FOCAL VERIFIED AT NECROPSY: OBSERVED -DILATATION, PELVIC,-PRESENT >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED -MINERALIZATION, PELVIC,-MODERATE, AT NECROPSY: NOT APPLICABLE MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 11 MM IN DIAMETER (FOR -HEMORRHAGE,-SLIGHT, MULTI-FOCAL COMPARISON, RIGHT IS 5 X 3 X 3 MM) -VACUOLATION,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT THYMUS (TH) : -CYST,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL 2484 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 883 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91142 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/14/99 STUDY DAY OF DEATH: 505 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 364.2 GRAMS DATE AND TIME OF NECROPSY: 06/14/99 14:48 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 9 X 7 X 5 MM, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL RED, DARK RED, TAN, LOBULATED AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -B-ADENOMA,-NEOPLASM PRESENT SINGLE MASS, 25 X 20 X 10 MM, RED, TAN, VASCULAR AND FIRM; ON SECTION EXUDES A TAN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 20) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW MOIST MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED DRY MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DARK RED AND BROWN, DRY, MATERIAL -------------------------------------------- 2485 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 884 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91142 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/14/99 STUDY DAY OF DEATH: 505 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 364.2 GRAMS DATE AND TIME OF NECROPSY: 06/14/99 14:48 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, INGUINAL (IN) ------------------------------------------------------------------------------------------------------------------------------------ 2486 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 885 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91143 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 174.0 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 7:25 PROSECTOR: DAVID SCHUETTE RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 6.8425 3.9325 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2487 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 886 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91143 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 174.0 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 7:25 PROSECTOR: DAVID SCHUETTE RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2488 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 887 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91144 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 320.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:15 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >DISCHARGE; GENITAL; RED IN COLOR; LIVER, OIL RED O (LI1) : VERIFIED AT NECROPSY: OBSERVED -POSITIVE OIL RED O STAINING,-MINIMAL, >APPEARANCE; SWOLLEN; VENTRAL- MULTI-FOCAL ABDOMINAL; VERIFIED AT NECROPSY: NOT KIDNEY (KD) : OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR -CYST,-PRESENT FLUID FILLED CYST ON EACH, LEFT IS 3 MM IN DIAMETER, RIGHT IS 10 MM IN DIAMETER BONE, STERNUM (SB) : -HYPERPLASIA, CARTILAGE,-MODERATELY SEVERE, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 25 X 15 X 12 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED 2489 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 888 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91144 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 320.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:15 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CERVIX (CV) : CERVIX (CV) : -LARGE; TRIMMING COMMENT: UP TO 8 MM -CYST,-PRESENT IN DIAMETER; TRIMMED IN ROUTINE SECTION UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: -DILATATION,-SEVERE, DIFFUSE REDDISH-BROWN FLUID -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- -LARGE, DIFFUSE; BILATERAL: EACH HORN FOCAL IS UP TO 110 X 25 X 25 MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST AND RED -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2490 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 889 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91144 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 320.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:15 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2491 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 890 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91145 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 264.9 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:07 PROSECTOR: DONIA MEYER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0402 .0152 % .0195 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0359 .0136 % .0174 WEIGHT TAKEN BRAIN (BR) 2.0585 .7771 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) .9720 .3669 % .4722 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.0001 .3775 % .4858 WEIGHT TAKEN LIVER (LI) 7.5230 2.8399 % 3.6546 WEIGHT TAKEN LUNG (LU) 1.4443 .5452 % .7016 WEIGHT TAKEN LEFT OVARY (OV0) .0866 .0327 % .0421 WEIGHT TAKEN RIGHT OVARY (OV1) .0878 .0331 % .0427 WEIGHT TAKEN SPLEEN (SP) .5149 .1944 % .2501 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0106 .0040 % .0051 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0085 .0032 % .0041 WEIGHT TAKEN UTERUS (UT) .7386 .2788 % .3588 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; >NOT REQUIRED TO BE EXAMINED FOR ANIMAL COLLECTED GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 2492 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 891 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91145 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 264.9 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:07 PROSECTOR: DONIA MEYER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2493 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 892 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91146 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 361.1 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:04 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); AXILLARY REGION-RIGHT; WEEK -DILATATION, VENTRICULAR,-PRESENT 71; 10 X 15MM; VERIFIED AT NECROPSY: KIDNEY (KD) : OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >APPEARANCE; HEAD TILT; LEFT; VERIFIED MINIMAL, MULTI-FOCAL AT NECROPSY: NOT APPLICABLE PANCREAS (PA) : >EYES; RED DISCHARGE; EYE-LEFT; -ATROPHY, ACINAR,-MODERATE, FOCAL VERIFIED AT NECROPSY: NOT OBSERVED ADRENAL, CORTEX (AC) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -ANGIECTASIS,-SLIGHT, MULTI-FOCAL NECROPSY: NOT OBSERVED -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 7 X 6 MM, -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI- FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : >TISSUE MISSING 2494 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 893 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91146 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 361.1 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:04 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, AXILLARY (AX) : LN, AXILLARY (AX) : -CYST(S); RIGHT, INVOLVING ENTIRE >NOTE:>AXILLARY LYMPH NODE CYSTS NODE: SINGLE CLEAR FLUID FILLED CYST, DESCRIBED GROSSLY WAS MAMMARY 10 MM IN DIAMETER; POSSIBLY CONSISTENT GALACTOCELES WITH MASS NOTED IN CLINICAL OBSERVATIONS (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR: BROKEN OFF AT BASE -------------------------------------------- 2495 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 894 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91146 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 361.1 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:04 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2496 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 895 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91147 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/14/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 323.9 GRAMS DATE AND TIME OF NECROPSY: 07/14/99 15:54 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: OBSERVED -LARGE PELVIS(ES); BILATERAL -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >EYES; RED DISCHARGE; EYES; VERIFIED AT -CYST(S); RIGHT, CORTICAL SURFACE: FOCAL NECROPSY: OBSERVED SINGLE CLEAR FLUID FILLED CYST, 3 MM -CYST,-MODERATE, FOCAL >APPEARANCE; HEAD TILT; RIGHT; VERIFIED IN DIAMETER -DILATATION, PELVIC,-PRESENT AT NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -VACUOLATION,-SLIGHT, FOCAL -B-ADENOMA,-NEOPLASM PRESENT LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -MOTTLED; RED AND TAN >UNREMARKABLE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 17 X 15 X 10 MM, DARK RED, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL 2497 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 896 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91147 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/14/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 323.9 GRAMS DATE AND TIME OF NECROPSY: 07/14/99 15:54 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); PERINEAL REGION: SINGLE DARK -B-ADENOMA,-NEOPLASM PRESENT RED FLUID FILLED CYST, 15 MM IN DIAMETER; COLLECTED UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -DILATATION,-MODERATE, DIFFUSE MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL, DARK RED AND DRY -ANIMAL THIN -------------------------------------------- 2498 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 897 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91147 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/14/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 323.9 GRAMS DATE AND TIME OF NECROPSY: 07/14/99 15:54 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2499 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 898 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91148 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 598.5 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:07 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LIVER (LI) : LEFT; VERIFIED AT NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR,-MODERATE, >MASS(ES); VENTRAL-ABDOMINAL; WEEK 95; MULTI-FOCAL 20 X 20MM; VERIFIED AT NECROPSY: LIVER, OIL RED O (LI1) : OBSERVED -POSITIVE OIL RED O STAINING,-SLIGHT, >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; MULTI-FOCAL WEEK 98; 30 X 25MM; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-SLIGHT, MULTI- >MASS(ES); INGUINAL REGION-RIGHT; WEEK FOCAL 98; 40 X 50MM; VERIFIED AT NECROPSY: ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : OBSERVED -DIFFUSELY DARK; RIGHT: DARK RED -DEGENERATION, CYSTIC, CORTICAL,- >MASS(ES); AXILLARY REGION-RIGHT; WEEK MODERATELY SEVERE, MULTI-FOCAL 102; 50 X 45MM; VERIFIED AT NECROPSY: -HEMORRHAGE,-MODERATE, MULTI-FOCAL OBSERVED -THROMBUS,-SLIGHT, FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE GREEN FLUID -CYST,-PRESENT FILLED CYST, 14 MM IN DIAMETER LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL 2500 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 899 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91148 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 598.5 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:07 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: SINGLE >UNREMARKABLE DARK RED LINEAR AREA, 6 X 1 MM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL REGION: -B-ADENOMA,-NEOPLASM PRESENT SINGLE MASS, 45 X 37 X 35 MM, RED, -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT DARK RED, TAN, FIRM AND LOBULATED; -M-CARCINOMA,-NEOPLASM PRESENT SAME ON CUT SURFACE; POSSIBLY CONSISTENT WITH MASS (AXILLARY REGION, RIGHT) AS NOTED IN CLINICAL OBSERVATIONS; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 21) -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 50 X 40 X 35 MM, RED, DARK RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL, RIGHT) AS NOTED IN CLINICAL OBSERVATIONS; COLLECTED; REGIONAL LN (SEE AXILLARY) -MASS #3; RIGHT INGUINAL REGION: SINGLE PEDUNCULATED MASS, 120 X 65 X 24 MM, RED, TAN AND LOBULATED; SAME ON CUT SURFACE; POSSIBLY CONSISTENT WITH MASSES (VENTRAL ABDOMINAL AND INGUINAL) AS NOTED IN CLINICAL OBSERVATIONS; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED 2501 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 900 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91148 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 598.5 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:07 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : -DILATATION,-MODERATELY SEVERE, FOCAL -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- SLIGHT, FOCAL -B-POLYP, ENDOMETRIAL, STROMAL,- NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; RIGHT: UP TO 10 X 8 X 3 MM -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, (LEFT IS 8 X 4 X 2 MM) (CAP 20) MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: -EROSION,-MODERATE, FOCAL SINGLE DARK RED CRUSTED AREA, 10 X 8 -EXOSTOSES,-MODERATE, MULTI-FOCAL MM; COLLECTED -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2502 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 901 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91148 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 598.5 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:07 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2503 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 902 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91149 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 360.4 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 13:10 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; BRAIN (BR) : BRAIN (BR) : WEEK 51; 50 X 55MM; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); AXILLARY REGION-LEFT; WEEK PANCREAS (PA) : 82; 35 X 30MM; VERIFIED AT NECROPSY: -ATROPHY, ACINAR,-MINIMAL, FOCAL OBSERVED ADRENAL, CORTEX (AC) : >SKIN & PELAGE; SORE/SCAB; LIMB-FRONT- -ANGIECTASIS,-MINIMAL, MULTI-FOCAL LEFT; VERIFIED AT NECROPSY: OBSERVED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL >APPEARANCE; THIN; VERIFIED AT LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : NECROPSY: OBSERVED >NOTE:>TRIMMING COMMENT: MISSING FROM >TISSUE MISSING WET TISSUE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 7 X 7 MM, RED, DARK -HEMORRHAGE,-SLIGHT, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -HEMORRHAGE,-MODERATE, MULTI-FOCAL SINGLE MASS, 20 X 16 X 8 MM, RED, DARK -PIGMENT,-MODERATE, MULTI-FOCAL RED, TAN AND FIRM; SAME ON CUT -B-FIBROADENOMA,-NEOPLASM PRESENT SURFACE; OVERLYING SKIN HAS A DARK RED -M-CARCINOMA,-NEOPLASM PRESENT AND TAN ERODED AREA, 9 X 5 MM (CAP 22); REGIONAL LN (AXILLARY) (CAP 21); OVERLYING SKIN, POSSIBLY CONSISTENT WITH SORE/SCAB (LIMB-FRONT-LEFT) NOTED IN CLINICAL OBSERVATIONS 2504 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 903 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91149 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 360.4 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 13:10 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 55 X 50 X 35 MM (40.8 GRAMS), RED, TAN, GRAY, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2505 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 904 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91149 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 360.4 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 13:10 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2506 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 905 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91150 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/05/99 STUDY DAY OF DEATH: 526 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 381.6 GRAMS DATE AND TIME OF NECROPSY: 07/05/99 10:33 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL; WEEK 62; KIDNEY (KD) : 45 X 60MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MINIMAL, FOCAL OBSERVED THYROID (TY) : >SKIN & PELAGE; BLUE SKIN; VENTRAL- -CYST, ULTIMOBRANCHIAL,-PRESENT CERVICAL; VERIFIED AT NECROPSY: NOT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : APPLICABLE -MASS(ES); VENTRAL MID THORACIC -GALACTOCELE,-MINIMAL, MULTI-FOCAL >RESPIRATION; LABORED; VERIFIED AT REGION: SINGLE MASS, 50 X 40 X 10 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL NECROPSY: NOT APPLICABLE RED, DARK RED, TAN, BROWN AND SOFT; -B-FIBROADENOMA,-NEOPLASM PRESENT >EYES; PALE; EYES; VERIFIED AT SAME ON CUT SURFACE; SOME AREAS EXUDE NECROPSY: NOT APPLICABLE A BROWN CASEOUS MATERIAL; OVERLYING SKIN HAS A SINGLE ABRADED AREA, 10 X 6 MM; COLLECTED; REGIONAL LN (SEE MANDIBULAR) CAUSE OF DEATH (CD) : -MAMMARY FIBROADENOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: COLLECTED >UNREMARKABLE LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; LEFT: 10 X 4 X 3 MM (FOR -HYPERPLASIA, LYMPHOCYTIC,-MINIMAL, COMPARISON, RIGHT IS 8 X 3 X 2 MM) MULTI-FOCAL (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2507 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 906 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91150 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/05/99 STUDY DAY OF DEATH: 526 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 381.6 GRAMS DATE AND TIME OF NECROPSY: 07/05/99 10:33 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2508 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 907 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91151 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 297.9 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:07 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR,- >APPEARANCE; HEAD TILT; LEFT; VERIFIED MODERATELY SEVERE, MULTI-FOCAL AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -MINERALIZATION, PELVIC,-SLIGHT, MULTI- NECROPSY: OBSERVED FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATELY SEVERE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, TAN, RED AND -B-ADENOMA,-NEOPLASM PRESENT FIRM STOMACH, GL (ST) : STOMACH, GL (ST) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE TAN, SLIGHTLY RAISED AREAS, UP TO 2 X 1 MM 2509 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 908 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91151 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 297.9 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:07 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE TAN, SLIGHTLY RAISED AREAS, UP TO 2 X 1 MM MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE GREEN MUCOID MATERIAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, DARK RED MATERIAL -ANIMAL THIN -------------------------------------------- 2510 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 909 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91151 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 297.9 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:07 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2511 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 910 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91152 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 402.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:33 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0403 .0100 % .0175 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0506 .0126 % .0220 WEIGHT TAKEN BRAIN (BR) 2.3005 .5717 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.3529 .3362 % .5881 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.3051 .3243 % .5673 WEIGHT TAKEN LIVER (LI) 11.2455 2.7946 % 4.8883 WEIGHT TAKEN LUNG (LU) 1.6125 .4007 % .7009 WEIGHT TAKEN LEFT OVARY (OV0) .0625 .0155 % .0272 WEIGHT TAKEN RIGHT OVARY (OV1) .0565 .0140 % .0246 WEIGHT TAKEN SPLEEN (SP) .6141 .1526 % .2669 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0144 .0036 % .0063 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0163 .0041 % .0071 WEIGHT TAKEN UTERUS (UT) 1.2985 .3227 % .5644 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -MOTTLED; ALL LOBES: BROWN AND TAN >UNREMARKABLE KIDNEY (KD) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -MINERALIZATION, TUBULAR,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT 2512 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 911 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91152 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 402.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:33 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -DILATATION,-MODERATE, MULTI-FOCAL MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2513 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 912 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91152 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 402.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:33 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2514 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 913 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91153 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 700.3 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 15:31 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 60; 40 X 25MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; SORE/SCAB; AXILLARY LIVER (LI) : LIVER (LI) : REGION-RIGHT; VERIFIED AT NECROPSY: -RED FOCUS(I)/AREA(S); ALL LOBES: -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- OBSERVED MULTIPLE DARK RED AREAS, UP TO 3 X 5 FOCAL >SKIN & PELAGE; PALE; ENTIRE BODY; MM; COLLECTED ON ROUTINE SECTIONS -NECROSIS, HEPATOCELLULAR,-SLIGHT, VERIFIED AT NECROPSY: NOT APPLICABLE MULTI-FOCAL -TELANGIECTASIS,-MODERATE, MULTI-FOCAL KIDNEY (KD) : KIDNEY (KD) : -GRANULAR MATERIAL; BILATERAL, WITHIN -MINERALIZATION, PELVIC,-MODERATE, PELVIS: MULTIPLE PINPOINT TAN GRANULES MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 4 X 6 X 8 MM, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- SOFT, RED, DARK RED AND TAN FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL 2515 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 914 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91153 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 700.3 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 15:31 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : -ULCERATION,-MODERATE, MULTI-FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL SINGLE MASS, 20 X 32 X 50 MM, FIRM, -NECROSIS,-MODERATELY SEVERE, MULTI- LOBULATED, RED, DARK RED, TAN; SAME ON FOCAL CUT SURFACE; COLLECTED; OVERLYING SKIN -M-CARCINOMA,-NEOPLASM PRESENT HAS A MOIST, RED AND BROWN ERODED AREA, 7 X 12 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (SEE AXILLARY) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE WHITE FLUID FILLED CYSTS, UP TO 2 MM IN DIAMETER (CAP 22) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED WITH ROUTINE SECTION CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 21) -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 20) -INFILTRATE, MACROPHAGE, PIGMENTED,- MODERATE, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2516 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 915 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91153 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 700.3 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 15:31 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2517 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 916 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91154 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/20/99 STUDY DAY OF DEATH: 541 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/20/99 14:33 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYE-RIGHT; BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -HEMORRHAGE,-MODERATE, MULTI-FOCAL -M-ASTROCYTOMA,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT CAUSE OF DEATH (CD) : -ASTROCYTOMA,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY, CRUSTED MATERIAL -------------------------------------------- 2518 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 917 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91154 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/20/99 STUDY DAY OF DEATH: 541 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/20/99 14:33 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2519 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 918 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91155 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 276.4 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:10 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0403 .0146 % .0214 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0436 .0158 % .0232 WEIGHT TAKEN BRAIN (BR) 1.8827 .6812 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.0812 .3912 % .5743 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.1129 .4026 % .5911 WEIGHT TAKEN LIVER (LI) 8.6177 3.1178 % 4.5773 WEIGHT TAKEN LUNG (LU) 1.1623 .4205 % .6174 WEIGHT TAKEN LEFT OVARY (OV0) .0753 .0272 % .0400 WEIGHT TAKEN RIGHT OVARY (OV1) .0530 .0192 % .0282 WEIGHT TAKEN SPLEEN (SP) .4785 .1731 % .2542 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0163 .0059 % .0087 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0099 .0036 % .0053 WEIGHT TAKEN UTERUS (UT) .7963 .2881 % .4230 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2520 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 919 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91155 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 276.4 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:10 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2521 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 920 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91156 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 575.0 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:42 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : RIGHT; VERIFIED AT NECROPSY: NOT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; RED HAIR COAT; PERINEAL LIVER (LI) : AREA; VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- >EXCRETION; FEW FECES; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >EYES; PALE; EYES; VERIFIED AT -MINERALIZATION, PELVIC,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- SLIGHT, FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 8 X 8 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL DARK RED, GRAY AND SOFT -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: -M-HEMANGIOSARCOMA,-NEOPLASM PRESENT DARK RED FLUID 2522 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 921 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91156 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 575.0 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:42 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : -LARGE, DIFFUSE; BILATERAL: EACH HORN IS DISTENDED UP TO 15 MM IN DIAMETER WITH A DARK RED FLUID -MASS(ES); ATTACHED TO SEROSAL SURFACE OF PROXIMAL LEFT HORN: SINGLE MASS, 15 MM IN DIAMETER, DARK RED, SOFT AND LOBULATED; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 20) CAUSE OF DEATH (CD) : -HEMANGIOSARCOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; RED -BLOOD FILM TAKEN -------------------------------------------- 2523 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 922 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91156 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 575.0 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:42 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 2524 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 923 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91157 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 387.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:29 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -EDEMA,-MODERATE, FOCAL MARROW, FEMUR (FM) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT CERVIX (CV) : CERVIX (CV) : -LARGE; 10 MM IN DIAMETER >UNREMARKABLE UTERUS (UT) : UTERUS (UT) : -CYST(S); MID-PORTION OF LEFT HORN, -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- WITHIN WALL: SINGLE CLEAR FLUID FILLED MODERATE, MULTI-FOCAL CYST, 3 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN 2525 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 924 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91157 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 387.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:29 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2526 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 925 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91158 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 708.1 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:10 PROSECTOR: JODI THIEREN RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 68; 110 X 90MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; -RED FOCUS(I)/AREA(S); ALL LOBES: -FOCUS, ALTERED HEPATOCELLULAR, VERIFIED AT NECROPSY: OBSERVED MULTIPLE DARK RED FOCI, UP TO 4 MM IN EOSINOPHILIC,-MODERATE, FOCAL DIAMETER; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -VACUOLATION,-MODERATE, FOCAL THYMUS (TH) : -CYST,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, FOCAL 2527 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 926 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91158 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 708.1 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:10 PROSECTOR: JODI THIEREN RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 5 X 4 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GREY AND SOFT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL SKIN (SK) : -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL -ULCERATION,-MODERATELY SEVERE, MULTI- FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 115 X 95 X 65 MM (221.5 -B-FIBROADENOMA,-NEOPLASM PRESENT GRAMS), RED, GREY, TAN, YELLOW, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION UTERUS (UT) : UTERUS (UT) : -MASS(ES); RIGHT, WITHIN LUMEN, -DILATATION,-SEVERE, FOCAL EXTENDING INTO CERVIX: SINGLE MASS, 42 -B-POLYP, ENDOMETRIAL, STROMAL,- X 11 X 11 MM, RED, DARK RED, TAN AND NEOPLASM PRESENT FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE LUMBAR) CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2528 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 927 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91158 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 708.1 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:10 PROSECTOR: JODI THIEREN RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; RIGHT: 7 X 5 X 2 MM (LEFT IS 5 -DILATATION, SINUSOIDAL,-MODERATE, X 2 X 2 MM) MULTI-FOCAL -MOTTLED; RIGHT: DARK RED AND TAN (CAP -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 22) LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP >UNREMARKABLE 21) FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); BILATERAL HINDFEET: -EROSION,-MINIMAL, FOCAL SINGLE DRY, RED CRUSTED FOCI ON EACH, -FIBROSIS,-MINIMAL, MULTI-FOCAL EACH IS 3 MM IN DIAMETER; BOTH -INFLAMMATION, ACUTE,-MINIMAL, MULTI- COLLECTED FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2529 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 928 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91158 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 708.1 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:10 PROSECTOR: JODI THIEREN RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2530 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 929 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91159 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 573.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:33 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK LIVER (LI) : LIVER (LI) : 85; 20 X 20MM; VERIFIED AT NECROPSY: -MOTTLED; ALL LOBES: DARK RED AND TAN -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- OBSERVED FOCAL LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 MM IN DIAMETER (RIGHT -ANGIECTASIS,-MODERATELY SEVERE, MULTI- IS 4 MM IN DIAMETER) FOCAL -HEMORRHAGE,-MODERATELY SEVERE, MULTI- FOCAL -THROMBUS,-SLIGHT, MULTI-FOCAL LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY DARK; DARK GREEN -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); LEFT ASPECT: -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SINGLE DARK BROWN FOCUS, 2 MM IN -B-ADENOMA,-NEOPLASM PRESENT DIAMETER MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT 2531 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 930 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91159 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 573.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:33 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 30 X 20 X 15 MM, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION URINARY BLADDER (UB) : -B-LEIOMYOMA,-NEOPLASM PRESENT UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN LUMEN: -DILATATION,-MINIMAL, DIFFUSE MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 20 X 7 X 7 MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2532 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 931 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91159 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 573.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:33 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2533 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 932 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91160 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 253.1 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:55 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0360 .0142 % .0174 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0349 .0138 % .0169 WEIGHT TAKEN BRAIN (BR) 2.0631 .8151 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.0818 .4274 % .5244 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.1055 .4368 % .5358 WEIGHT TAKEN LIVER (LI) 7.7748 3.0718 % 3.7685 WEIGHT TAKEN LUNG (LU) 1.0340 .4085 % .5012 WEIGHT TAKEN LEFT OVARY (OV0) .0710 .0281 % .0344 WEIGHT TAKEN RIGHT OVARY (OV1) .0450 .0178 % .0218 WEIGHT TAKEN SPLEEN (SP) .3912 .1546 % .1896 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0086 .0034 % .0042 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0095 .0038 % .0046 WEIGHT TAKEN UTERUS (UT) .6152 .2431 % .2982 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; STOMACH, GL (ST) : STOMACH, GL (ST) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); MUCOSA: SINGLE >NOT REQUIRED TO BE EXAMINED FOR ANIMAL DARK RED AREA, 2 X 1 MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 2534 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 933 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91160 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 253.1 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:55 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2535 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 934 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91161 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/22/99 STUDY DAY OF DEATH: 421 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 265.4 GRAMS DATE AND TIME OF NECROPSY: 03/22/99 13:48 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; HEAD- BRAIN (BR) : BRAIN (BR) : ENTIRE; VERIFIED AT NECROPSY: NOT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MINIMAL, MULTI- >APPEARANCE; THIN; VERIFIED AT FOCAL NECROPSY: OBSERVED SPLEEN (SP) : SPLEEN (SP) : >APPEARANCE; HEAD TILT; RIGHT; VERIFIED -SMALL; 3 X 5 X 29 MM -PIGMENT, INCREASED,-SLIGHT, MULTI- AT NECROPSY: NOT APPLICABLE FOCAL OVARY (OV) : -CYST,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 8 X 13 MM, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- SOFT, RED, DARK RED, AND TAN FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN 2536 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 935 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91161 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/22/99 STUDY DAY OF DEATH: 421 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 265.4 GRAMS DATE AND TIME OF NECROPSY: 03/22/99 13:48 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL INFORMATION (XX) : >NOTE:>FINAL BODY WEIGHT WAS 265.4 GRAMS; NOT ENTERED AT TIME DEATH CODE WAS ASSIGNED -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2537 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 936 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91162 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 225.0 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:31 PROSECTOR: WENDY CHERRY RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0350 .0156 % .0185 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0338 .0150 % .0179 WEIGHT TAKEN BRAIN (BR) 1.8884 .8393 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) .7299 .3244 % .3865 WEIGHT TAKEN RIGHT KIDNEY (KD1) .7633 .3392 % .4042 WEIGHT TAKEN LIVER (LI) 6.5271 2.9009 % 3.4564 WEIGHT TAKEN LUNG (LU) 1.0618 .4719 % .5623 WEIGHT TAKEN LEFT OVARY (OV0) .0498 .0221 % .0264 WEIGHT TAKEN RIGHT OVARY (OV1) .0596 .0265 % .0316 WEIGHT TAKEN SPLEEN (SP) .4378 .1946 % .2318 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0071 .0032 % .0038 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0077 .0034 % .0041 WEIGHT TAKEN UTERUS (UT) .6084 .2704 % .3222 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2538 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 937 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91162 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 225.0 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:31 PROSECTOR: WENDY CHERRY RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2539 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 938 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91163 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 266.4 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 9:51 PROSECTOR: WENDY CHERRY RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0341 .0128 % .0159 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0334 .0125 % .0156 WEIGHT TAKEN BRAIN (BR) 2.1433 .8045 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) .9469 .3554 % .4418 WEIGHT TAKEN RIGHT KIDNEY (KD1) .9284 .3485 % .4332 WEIGHT TAKEN LIVER (LI) 7.0599 2.6501 % 3.2939 WEIGHT TAKEN LUNG (LU) 1.2105 .4544 % .5648 WEIGHT TAKEN LEFT OVARY (OV0) .0679 .0255 % .0317 WEIGHT TAKEN RIGHT OVARY (OV1) .0764 .0287 % .0356 WEIGHT TAKEN SPLEEN (SP) .5740 .2155 % .2678 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0056 .0021 % .0026 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0040 .0015 % .0019 WEIGHT TAKEN UTERUS (UT) .6644 .2494 % .3100 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2540 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 939 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91163 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 266.4 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 9:51 PROSECTOR: WENDY CHERRY RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2541 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 940 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91164 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 659 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 481.0 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:52 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 86; 20 X 25MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- >SKIN & PELAGE; YELLOW HAIR COAT; FOCAL VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION,-SLIGHT, FOCAL >EXCRETION; FEW FECES; VERIFIED AT -TELANGIECTASIS,-MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >APPEARANCE; HUNCHED; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -RED FOCUS(I)/AREA(S); LEFT: SINGLE -HEMORRHAGE,-MINIMAL, MULTI-FOCAL DARK RED AREA, 3 X 2 MM THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 7 MM, RED, DARK -HEMORRHAGE,-SLIGHT, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT 2542 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 941 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91164 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 659 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 481.0 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:52 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 35 X 20 X 15 MM, RED, DARK RED, TAN, SOFT AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED, DRY MATERIAL -------------------------------------------- 2543 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 942 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91164 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 659 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 481.0 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:52 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2544 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 943 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91165 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 228.4 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:45 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 6.8342 2.9922 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2545 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 944 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91165 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 228.4 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:45 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2546 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 945 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91166 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 577.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:03 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -TELANGIECTASIS,-MODERATE, MULTI-FOCAL MULTIPLE AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI- FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -LARGE; DIFFUSE: UP TO 5 MM IN >UNREMARKABLE DIAMETER LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL 2547 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 946 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91166 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 577.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:03 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -FIBROSIS,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 5 X 4 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, GRAY, LOBULATED, AND SOFT -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : >NOTE:>SECTION TAKEN FROM RIGHT SIDE DUE TO MAMMARY GLAND MASS MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL SINGLE MASS, 57 X 40 X 23 MM, RED, -B-FIBROADENOMA,-NEOPLASM PRESENT TAN, LOBULATED, AND SOFT; ON SECTION EXUDES A TAN CREAMY SEMISOLID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION >NOTE:>SECTION TAKEN FROM RIGHT SIDE DUE TO MAMMARY GLAND MASS UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN LUMEN: -DILATATION,-MODERATELY SEVERE, DIFFUSE MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 8 X 5 X 5 MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2548 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 947 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91166 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 577.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:03 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -HEMORRHAGE,-SLIGHT, MULTI-FOCAL (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2549 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 948 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91167 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 717 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 10:03 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); DORSAL; WEEK 82; 40 X 45MM; KIDNEY (KD) : KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT >APPEARANCE; LIMITED USE; PAW-HIND- -CALCULUS(I); BILATERAL, WITHIN -MINERALIZATION, TUBULAR,-MINIMAL, LEFT; VERIFIED AT NECROPSY: NOT PELVIS: MULTIPLE TAN AND FIRM CALCULI, MULTI-FOCAL APPLICABLE UP TO 4 MM IN DIAMETER -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL PANCREAS (PA) : -HYPERPLASIA, ISLET CELLS,-SLIGHT, FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 5 MM IN DIAMETER THYMUS (TH) : -CYST,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL 2550 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 949 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91167 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 717 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 10:03 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -FIBROSIS,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 6 MM, DARK RED, -CYST,-PRESENT TAN, AND SOFT -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : JEJUNUM (JE) : -DIVERTICULUM(A); MID JEJUNUM: SINGLE -B-ANGIOMA,-NEOPLASM PRESENT DARK RED AND TAN AREA, 12 X 10 X 10 MM; COLLECTED ON ROUTINE SECTION MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 60 X 45 X 30 MM, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) UTERUS (UT) : UTERUS (UT) : -CYST(S); LEFT, WITHIN WALL: SINGLE -DILATATION,-MODERATE, FOCAL CLEAR FLUID FILLED CYST, 5 X 4 X 4 MM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; LEFT TARSAL JOINT: UP TO 15 MM -EXOSTOSES,-MODERATE, MULTI-FOCAL IN DIAMETER; COLLECTED -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL 2551 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 950 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91167 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 717 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 10:03 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2552 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 951 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91168 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/05/99 STUDY DAY OF DEATH: 588 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 294.4 GRAMS DATE AND TIME OF NECROPSY: 09/05/99 16:29 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; YELLOW HAIR COAT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED -DILATATION, VENTRICULAR,-PRESENT AT NECROPSY: NOT APPLICABLE LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, NECROPSY: OBSERVED EOSINOPHILIC,-MINIMAL, FOCAL >EXCRETION; NO FECES; VERIFIED AT -VACUOLATION, HEPATOCELLULAR,-MINIMAL, NECROPSY: NOT APPLICABLE FOCAL >APPEARANCE; HEAD TILT; RIGHT; VERIFIED KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYROID (TY) : THYROID (TY) : -MASS(ES); INVOLVING ENTIRE LEFT -CYST, ULTIMOBRANCHIAL,-PRESENT THYROID: SINGLE MASS, 8 X 5 X 5 MM, -M-CARCINOMA, C-CELL,-NEOPLASM PRESENT TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT 2553 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 952 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91168 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/05/99 STUDY DAY OF DEATH: 588 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 294.4 GRAMS DATE AND TIME OF NECROPSY: 09/05/99 16:29 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 6 X 6 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN AND FIRM -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -C-CELL CARCINOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST BROWN MATERIAL -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2554 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 953 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91168 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/05/99 STUDY DAY OF DEATH: 588 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 294.4 GRAMS DATE AND TIME OF NECROPSY: 09/05/99 16:29 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2555 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 954 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91169 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 199.6 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:19 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 6.3263 3.1695 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2556 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 955 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91169 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 199.6 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:19 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2557 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 956 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91170 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 258.8 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 14:33 PROSECTOR: JILL PAUS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >APPEARANCE; HEAD TILT; RIGHT; VERIFIED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- AT NECROPSY: NOT APPLICABLE FOCAL >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MODERATE, >APPEARANCE; HUNCHED; VERIFIED AT MULTI-FOCAL NECROPSY: NOT APPLICABLE PANCREAS (PA) : >SKIN & PELAGE; PALE; ENTIRE BODY; -PIGMENT,-MINIMAL, MULTI-FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -DIFFUSELY RED; RIGHT: DARK RED -DEGENERATION, CYSTIC, CORTICAL,- -LARGE; RIGHT: 7 MM IN DIAMETER (LEFT MODERATELY SEVERE, MULTI-FOCAL IS 4 X 3 X 2 MM) -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, MULTI-FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -CYST,-PRESENT 2558 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 957 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91170 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 258.8 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 14:33 PROSECTOR: JILL PAUS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 7 X 5 MM, RED, DARK -HEMORRHAGE,-MODERATELY SEVERE, MULTI- RED, TAN, SOFT, AND LOBULATED FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -PIGMENT,-MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; LEFT: 11 X 7 X 3 MM (RIGHT IS -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, 4 X 3 X 2 MM ); COLLECTED DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER AND LOWER INCISORS -ANIMAL THIN -------------------------------------------- 2559 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 958 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91170 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 258.8 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 14:33 PROSECTOR: JILL PAUS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2560 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 959 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91171 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 186.2 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:39 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 5.8384 3.1356 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2561 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 960 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91171 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 186.2 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:39 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2562 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 961 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91172 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 456.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:59 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT EYE (EY) : NECROPSY: NOT OBSERVED -ULCERATION, CORNEAL,-MINIMAL, FOCAL >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >APPEARANCE; MISSING; TEETH; VERIFIED MULTIPLE RED AREAS, UP TO 5 X 4 MM; FOCAL AT NECROPSY: OBSERVED COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL -HYPERPLASIA, ACINAR,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL 2563 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 962 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91172 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 456.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:59 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -GALACTOCELE,-SLIGHT, MULTI-FOCAL AND FIRM; COLLECTED ON ROUTINE SECTION UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -DILATATION,-MODERATELY SEVERE, MULTI- MULTIPLE CLEAR FLUID FILLED CYSTS, UP FOCAL TO 3 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); BILATERAL, HINDFEET: -EDEMA,-MODERATE, MULTI-FOCAL SINGLE RED AND TAN CRUSTED AREA ON -EROSION,-MODERATE, MULTI-FOCAL EACH, EACH IS 3 MM IN DIAMETER; BOTH -INFLAMMATION, ACUTE,-MODERATE, MULTI- COLLECTED FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN AT BASE -------------------------------------------- 2564 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 963 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91172 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 456.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:59 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2565 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 964 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91173 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/05/99 STUDY DAY OF DEATH: 404 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/05/99 10:23 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; SQUINTED; PERI-ORBITAL; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT OBSERVED KIDNEY (KD) : >DISCHARGE; NASAL; RED IN COLOR; -MINERALIZATION, TUBULAR,-MINIMAL, VERIFIED AT NECROPSY: OBSERVED MULTI-FOCAL >SKIN & PELAGE; YELLOW HAIR COAT; ADRENAL, CORTEX (AC) : VERIFIED AT NECROPSY: NOT OBSERVED -ANGIECTASIS,-MINIMAL, MULTI-FOCAL >EYES; RED DISCHARGE; EYES; VERIFIED AT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL NECROPSY: OBSERVED THYROID (TY) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM NECROPSY: NOT APPLICABLE PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 MM IN DIAMETER, DARK RED -HEMORRHAGE,-MODERATELY SEVERE, MULTI- AND TAN FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MINIMAL, FOCAL SKIN (SK) : -CYST, EPIDERMAL INCLUSION,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: -GALACTOCELE,-MODERATELY SEVERE, MULTI- MULTIPLE TAN AND CREAMY FLUID FILLED FOCAL CYSTS, UP TO 6 MM IN DIAMETER (CAP 20) 2566 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 965 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91173 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/05/99 STUDY DAY OF DEATH: 404 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/05/99 10:23 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED -DISCHARGE-NASAL; BILATERAL: DARK RED -------------------------------------------- 2567 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 966 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91173 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/05/99 STUDY DAY OF DEATH: 404 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/05/99 10:23 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2568 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 967 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91174 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 256.6 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:05 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0350 .0136 % .0173 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0298 .0116 % .0148 WEIGHT TAKEN BRAIN (BR) 2.0196 .7871 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.0395 .4051 % .5147 WEIGHT TAKEN RIGHT KIDNEY (KD1) .9432 .3676 % .4670 WEIGHT TAKEN LIVER (LI) 7.3862 2.8785 % 3.6573 WEIGHT TAKEN LUNG (LU) 1.2895 .5025 % .6385 WEIGHT TAKEN LEFT OVARY (OV0) .0904 .0352 % .0448 WEIGHT TAKEN RIGHT OVARY (OV1) .0654 .0255 % .0324 WEIGHT TAKEN SPLEEN (SP) .4707 .1834 % .2331 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0067 .0026 % .0033 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0060 .0023 % .0030 WEIGHT TAKEN UTERUS (UT) .5262 .2051 % .2605 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HEMORRHAGE,-MODERATE, FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE RED FLUID >UNREMARKABLE FILLED CYST, 8 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 2569 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 968 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91174 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 256.6 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:05 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS) ------------------------------------------------------------------------------------------------------------------------------------ 2570 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 969 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91175 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 514.4 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 15:00 PROSECTOR: TERRI L. WILLIAMS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 74; 50 X 45MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED KIDNEY (KD) : KIDNEY (KD) : >APPEARANCE; HEAD TILT; LEFT; VERIFIED -LARGE PELVIS(ES); RIGHT -DILATATION, PELVIC,-PRESENT AT NECROPSY: NOT APPLICABLE -GRANULAR MATERIAL; RIGHT, WITHIN -MINERALIZATION, PELVIC,-MODERATE, >EYES; RED DISCHARGE; EYE-LEFT; PELVIS: TAN GRANULAR MATERIAL MULTI-FOCAL VERIFIED AT NECROPSY: OBSERVED ADRENAL, CORTEX (AC) : >BEHAVIOR; ATAXIC; VERIFIED AT -ANGIECTASIS,-MODERATELY SEVERE, MULTI- NECROPSY: NOT APPLICABLE FOCAL -HEMORRHAGE,-MODERATELY SEVERE, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 7 X 6 MM, RED, DARK RED, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL MULTIPLE MASSES, UP TO 40 X 35 X 25 -B-FIBROADENOMA,-NEOPLASM PRESENT MM, ALL ARE RED, DARK RED, TAN, LOBULATED AND FIRM; SOME SECTIONS EXUDE A TAN SEMIFLUID; PORTION COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION 2571 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 970 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91175 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 514.4 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 15:00 PROSECTOR: TERRI L. WILLIAMS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: RED -------------------------------------------- 2572 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 971 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91175 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 514.4 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 15:00 PROSECTOR: TERRI L. WILLIAMS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2573 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 972 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91176 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 445.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:10 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; LIMITED USE; PAW-HIND- -DILATATION, VENTRICULAR,-PRESENT LEFT; VERIFIED AT NECROPSY: NOT LIVER (LI) : LIVER (LI) : APPLICABLE -ADHESION(S); RIGHT LATERAL LOBE TO -FIBROSIS, CAPSULAR,-MODERATE, FOCAL RIGHT MEDIAN LOBE: MULTIPLE TAN -HEMORRHAGE,-MODERATE, MULTI-FOCAL FIBROUS ADHESIONS; COLLECTED ON -NECROSIS, HEPATOCELLULAR,-MODERATELY ROUTINE SECTION SEVERE, FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 X 6 X 5 MM (RIGHT IS 4 -DEGENERATION, CYSTIC, CORTICAL,- X 3 X 2 MM) SEVERE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -VACUOLATION,-SLIGHT, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 7 X 6 MM, RED, DARK -HEMORRHAGE,-SLIGHT, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT 2574 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 973 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91176 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 445.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:10 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -EDEMA,-MODERATELY SEVERE, FOCAL STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE -EDEMA,-MODERATELY SEVERE, FOCAL BROWN ERODED AREA, 4 X 2 MM -EROSION,-MODERATELY SEVERE, FOCAL -THICKENED WALL; DIFFUSE SKIN (SK) : SKIN (SK) : >NOTE:>TRIMMING COMMENT: MISSING AT >TISSUE MISSING TRIMMING MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : >NOTE:>TRIMMING COMMENT: MISSING AT >TISSUE MISSING TRIMMING UTERUS (UT) : UTERUS (UT) : -MASS(ES); SEROSAL SURFACE OF LEFT -B-LEIOMYOMA,-NEOPLASM PRESENT HORN: SINGLE MASS, 4 X 3 X 3 MM, TAN AND FIRM; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 20) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; LEFT TARSAL JOINT: 20 X 9 X 8 -EXOSTOSES,-SLIGHT, MULTI-FOCAL MM (RIGHT IS 10 X 6 X 5 MM); COLLECTED -FIBROSIS,-MODERATE, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN 2575 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 974 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91176 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 445.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:10 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 2576 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 975 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91177 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 626.3 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:10 PROSECTOR: TONY HANFELD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK LIVER (LI) : 63; 130 X 110MM; VERIFIED AT NECROPSY: -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OBSERVED ADRENAL, CORTEX (AC) : >APPEARANCE; THIN; VERIFIED AT -ANGIECTASIS,-SLIGHT, MULTI-FOCAL NECROPSY: NOT OBSERVED -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT SKIN (SK) : -FAT NECROSIS,-MODERATE, MULTI-FOCAL -B-LIPOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: >UNREMARKABLE SINGLE MASS, 145 X 110 X 50 MM (274.3 GRAMS), TAN, RED, DARK RED, VASCULAR, LOBULATED AND SOFT; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE AXILLARY) CAUSE OF DEATH (CD) : -LIPOMA,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 11 X 6 X 3 MM >UNREMARKABLE (RIGHT IS UP TO 7 X 4 X 2 MM) (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2577 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 976 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91177 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 626.3 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:10 PROSECTOR: TONY HANFELD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2578 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 977 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91178 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 370.8 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 13:53 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >MASS(ES); VENTRAL-ABDOMINAL; WEEK 90; -MINERALIZATION, PELVIC,-SLIGHT, MULTI- 10 X 10MM; VERIFIED AT NECROPSY: NOT FOCAL OBSERVED ADRENAL, CORTEX (AC) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -ANGIECTASIS,-SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE -HEMORRHAGE,-SLIGHT, MULTI-FOCAL >APPEARANCE; HEAD TILT; LEFT; VERIFIED LN, MESENTERIC (MS) : AT NECROPSY: NOT APPLICABLE -INFILTRATE, MACROPHAGE, PIGMENTED,- >BEHAVIOR; ATAXIC; VERIFIED AT MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 5 MM IN DIAMETER THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : LUNG (LU) : -DIFFUSELY RED; ALL LOBES: DARK RED -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 7 MM, RED, DARK RED, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL TAN, LOBULATED, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT 2579 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 978 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91178 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 370.8 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 13:53 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL; COLLECTED >UNREMARKABLE LN, MEDIASTINAL (ML) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2580 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 979 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91178 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 370.8 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 13:53 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2581 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 980 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91179 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/15/98 STUDY DAY OF DEATH: 324 STUDY WEEK OF DEATH: 47 TERMINAL BODY WEIGHT: 370.2 GRAMS DATE AND TIME OF NECROPSY: 12/15/98 12:14 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; LIVER (LI) : WEEK 36; 65 X 50MM; VERIFIED AT -HEMATOPOIESIS, EXTRAMEDULLARY,- NECROPSY: OBSERVED MINIMAL, MULTI-FOCAL >MASS(ES); AXILLARY REGION-RIGHT; WEEK -NECROSIS, HEPATOCELLULAR,-MINIMAL, 38; 80 X 65MM; VERIFIED AT NECROPSY: MULTI-FOCAL OBSERVED SPLEEN (SP) : >SKIN & PELAGE; PALE; ENTIRE BODY; -HEMATOPOIESIS, EXTRAMEDULLARY, VERIFIED AT NECROPSY: NOT APPLICABLE INCREASED,-MODERATE, DIFFUSE >SKIN & PELAGE; SORE/SCAB; VENTRAL- PANCREAS (PA) : CERVICAL; VERIFIED AT NECROPSY: NOT -ATROPHY, ACINAR,-MODERATE, FOCAL OBSERVED ADRENAL, CORTEX (AC) : >APPEARANCE; THIN; VERIFIED AT -DEGENERATION, CYSTIC, CORTICAL,- NECROPSY: OBSERVED MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC,-MODERATE, DIFFUSE HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL MUSCLE, SKELETAL (SM) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT 2582 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 981 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91179 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/15/98 STUDY DAY OF DEATH: 324 STUDY WEEK OF DEATH: 47 TERMINAL BODY WEIGHT: 370.2 GRAMS DATE AND TIME OF NECROPSY: 12/15/98 12:14 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: -EDEMA,-MINIMAL, MULTI-FOCAL MULTIPLE TAN AREAS, 1 MM IN DIAMETER -EROSION,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL SINGLE MASS, 75 X 63 X 45 MM, RED, -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT DARK RED, TAN, FIRM AND LOBULATED; -M-FIBROSARCOMA,-NEOPLASM PRESENT SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 21) -CYST(S); VENTRAL CERVICAL REGION: MULTIPLE TAN AND CREAMY SEMI-FLUID FILLED CYSTS, UP TO 8 MM IN DIAMETER (CAP 22) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 60 X 60 X 20 MM, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -MAMMARY FIBROADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2583 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 982 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91179 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/15/98 STUDY DAY OF DEATH: 324 STUDY WEEK OF DEATH: 47 TERMINAL BODY WEIGHT: 370.2 GRAMS DATE AND TIME OF NECROPSY: 12/15/98 12:14 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2584 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 983 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91180 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/16/99 STUDY DAY OF DEATH: 446 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/16/99 13:36 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; BRAIN (BR) : BRAIN (BR) : WEEK 32; 150 X 120MM; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-SLIGHT, MULTI- FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, DIFFUSE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : -MINERALIZATION,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); RIGHT ANTERIOR -B-ADENOMA,-NEOPLASM PRESENT ASPECT: SINGLE BROWN LINEAR AREA, 3 X LESS THAN 1 MM -LARGE; 7 X 7 X 5 MM, APPEARS SWOLLEN 2585 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 984 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91180 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/16/99 STUDY DAY OF DEATH: 446 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/16/99 13:36 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -GALACTOCELE,-MODERATE, DIFFUSE SINGLE MASS, 120 X 90 X 40 MM (242.0 -MINERALIZATION,-MINIMAL, MULTI-FOCAL GRAMS), RED, DARK RED, TAN, FIRM, AND -B-FIBROADENOMA,-NEOPLASM PRESENT LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE INGUINAL) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, INGUINAL (IN) : LN, INGUINAL (IN) : -LARGE; RIGHT: 10 X 5 X 2 MM (FOR -HYPERPLASIA, LYMPHOCYTIC,-MINIMAL, COMPARISON, LEFT IS 5 X 2 X 1 MM) (CAP MULTI-FOCAL 20) -DIFFUSELY RED; BILATERAL -------------------------------------------- 2586 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 985 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91180 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/16/99 STUDY DAY OF DEATH: 446 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/16/99 13:36 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2587 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 986 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91181 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 302.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:31 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0283 .0093 % .0146 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0357 .0118 % .0185 WEIGHT TAKEN BRAIN (BR) 1.9333 .6385 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.0638 .3513 % .5503 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.0540 .3481 % .5452 WEIGHT TAKEN LIVER (LI) 9.3987 3.1039 % 4.8615 WEIGHT TAKEN LUNG (LU) 1.2459 .4115 % .6444 WEIGHT TAKEN LEFT OVARY (OV0) .0782 .0258 % .0404 WEIGHT TAKEN RIGHT OVARY (OV1) .0859 .0284 % .0444 WEIGHT TAKEN SPLEEN (SP) .4567 .1508 % .2362 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0079 .0026 % .0041 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0102 .0034 % .0053 WEIGHT TAKEN UTERUS (UT) .5781 .1909 % .2990 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2588 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 987 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91181 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 302.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:31 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2589 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 988 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91182 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 316.1 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 12:35 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; YELLOW HAIR COAT; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- >EXCRETION; FEW FECES; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >APPEARANCE; THIN; VERIFIED AT FOCAL NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR,-SLIGHT, >MASS(ES); INGUINAL REGION-LEFT; WEEK MULTI-FOCAL 87; 10 X 15MM; VERIFIED AT NECROPSY: KIDNEY (KD) : OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, MULTI- >BEHAVIOR; HYPOACTIVE; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE -PYELONEPHRITIS,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -RED FOCUS(I)/AREA(S); BILATERAL: -DEGENERATION, CYSTIC, CORTICAL,- MULTIPLE DARK RED, PINPOINT FOCI, UP MODERATE, MULTI-FOCAL TO 4 X 2 MM -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, FOCAL -THROMBUS,-SLIGHT, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 9 X 7 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, SOFT AND LOBULATED STOMACH, GL (ST) : -EROSION,-MINIMAL, MULTI-FOCAL 2590 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 989 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91182 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 316.1 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 12:35 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -HEMORRHAGE,-MODERATE, MULTI-FOCAL SINGLE MASS, 20 X 17 X 8 MM, RED, DARK -M-CARCINOMA,-NEOPLASM PRESENT RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY MATERIAL -ANIMAL THIN -------------------------------------------- 2591 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 990 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91182 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 316.1 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 12:35 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2592 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 991 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91183 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 596.5 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:15 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); LEFT -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-SLIGHT, MULTI- FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, FOCAL -THROMBUS,-MINIMAL, FOCAL THYMUS (TH) : -CYST,-PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -HEMORRHAGE,-SLIGHT, FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 5 X 5 MM, DARK RED, TAN -HEMORRHAGE,-MODERATE, MULTI-FOCAL AND FIRM -B-ADENOMA,-NEOPLASM PRESENT 2593 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 992 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91183 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 596.5 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:15 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: -GALACTOCELE,-SLIGHT, MULTI-FOCAL MULTIPLE TAN AND CREAMY SEMIFLUID -B-ADENOMA,-NEOPLASM PRESENT FILLED CYSTS, UP TO 4 MM IN DIAMETER -B-FIBROADENOMA,-NEOPLASM PRESENT (CAP 20) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION URINARY BLADDER (UB) : -M-CARCINOMA, TRANSITIONAL CELL,- NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT ADIPOSE TISSUE (AT) : ADIPOSE TISSUE (AT) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE -I-TRANSITIONAL CELL CARCINOMA,- NEAR URINARY BLADDER: SINGLE MASS, 22 NEOPLASM PRESENT X 12 X 10 MM, RED, DARK RED, LOBULATED AND VASCULAR; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (LUMBAR) NORMAL (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2594 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 993 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91183 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 596.5 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:15 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 2595 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 994 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91184 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/16/99 STUDY DAY OF DEATH: 629 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 456.9 GRAMS DATE AND TIME OF NECROPSY: 10/16/99 7:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN -VACUOLATION, HEPATOCELLULAR,-SEVERE, DIFFUSE KIDNEY (KD) : KIDNEY (KD) : -GRANULAR MATERIAL; RIGHT, WITHIN -CAST, PROTEINACEOUS,-MINIMAL, MULTI- PELVIS: MULTIPLE TAN AND FIRM GRANULES FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 MM IN DIAMETER (RIGHT -ANGIECTASIS,-MODERATE, MULTI-FOCAL IS 4 MM IN DIAMETER) -DEGENERATION, CYSTIC, CORTICAL,- MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 2596 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 995 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91184 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/16/99 STUDY DAY OF DEATH: 629 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 456.9 GRAMS DATE AND TIME OF NECROPSY: 10/16/99 7:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 9 X 8 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE >UNREMARKABLE DARK BROWN ERODED AREAS, UP TO 3 X 2 MM CECUM (CE) : CECUM (CE) : -SMALL; 30 X 6 X 6 MM >UNREMARKABLE MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -HYPERPLASIA, GLANDULAR,-SLIGHT, AND FIRM; COLLECTED ON ROUTINE DIFFUSE SECTIONS UTERUS (UT) : UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE -DILATATION,-MINIMAL, FOCAL VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE GREEN MUCOID SEMIFLUID MATERIAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: -EROSION,-MODERATE, FOCAL SINGLE DRY, DARK RED CRUSTED AREA, 10 -FIBROSIS,-SLIGHT, FOCAL X 8 MM; COLLECTED -INFLAMMATION, ACUTE,-MODERATELY SEVERE, FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; PERINEUM: DRY, YELLOW MATERIAL -BLOOD FILM TAKEN 2597 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 996 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91184 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/16/99 STUDY DAY OF DEATH: 629 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 456.9 GRAMS DATE AND TIME OF NECROPSY: 10/16/99 7:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2598 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 997 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91185 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/04/99 STUDY DAY OF DEATH: 464 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 217.6 GRAMS DATE AND TIME OF NECROPSY: 05/04/99 14:14 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; HUNCHED; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE KIDNEY (KD) : KIDNEY (KD) : >APPEARANCE; THIN; VERIFIED AT -MOTTLED; BILATERAL: BROWN AND DARK -MINERALIZATION, PELVIC,-MODERATE, NECROPSY: OBSERVED BROWN MULTI-FOCAL >SKIN & PELAGE; PALE; ENTIRE BODY; SPLEEN (SP) : VERIFIED AT NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-MODERATE, MULTI- >EXCRETION; FEW FECES; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE OVARY (OV) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 10 X 10 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, AND SOFT HARDERIAN GLAND (HG) : -ONE EXAMINED,-PRESENT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE, -EDEMA,-MINIMAL, FOCAL WHITE, ERODED AREAS, UP TO 4 X 3 MM -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT UTERUS (UT) : >UNREMARKABLE 2599 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 998 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91185 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/04/99 STUDY DAY OF DEATH: 464 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 217.6 GRAMS DATE AND TIME OF NECROPSY: 05/04/99 14:14 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 2 MM IN DIAMETER CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND CRUSTED -ANIMAL THIN -------------------------------------------- 2600 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 999 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91185 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/04/99 STUDY DAY OF DEATH: 464 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 217.6 GRAMS DATE AND TIME OF NECROPSY: 05/04/99 14:14 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2601 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1000 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91186 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 488.8 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 13:51 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 76; 90 X 80MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >SKIN & PELAGE; PALE; ENTIRE BODY; EOSINOPHILIC,-MINIMAL, FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 X 5 X 5 MM (RIGHT IS 4 -ANGIECTASIS,-SLIGHT, MULTI-FOCAL X 4 X 3 MM) -DEGENERATION, CYSTIC, CORTICAL,- MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 2602 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1001 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91186 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 488.8 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 13:51 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT, MULTI-FOCAL OVARY (OV) : -ONE EXAMINED,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 6 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- TAN AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL CECUM (CE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT ABDOMINAL REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL SINGLE MASS, 90 X 70 X 55 MM (205.9 -MINERALIZATION,-MODERATE, MULTI-FOCAL GRAMS), TAN, RED, SOFT AND VASCULAR; -B-FIBROADENOMA,-NEOPLASM PRESENT SAME ON CUT SURFACE; REGIONAL LN (INGUINAL) NOT IDENTIFIED -CYST(S); VENTRAL THORACIC REGION AND RIGHT INGUINAL REGION: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 15 X 10 X 10 MM (CAPS 21 AND 22) 2603 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1002 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91186 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 488.8 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 13:51 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND SOFT (CAP 23) CAUSE OF DEATH (CD) : -MAMMARY FIBROADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2604 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1003 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91186 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 488.8 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 13:51 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2605 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1004 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91187 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 257.8 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 14:45 PROSECTOR: TERRI L. WILLIAMS RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); AXILLARY REGION-LEFT; WEEK -DILATATION, VENTRICULAR,-PRESENT 71; 25 X 25MM; VERIFIED AT NECROPSY: KIDNEY (KD) : OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >APPEARANCE; HUNCHED; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE -MINERALIZATION, TUBULAR,-MINIMAL, >EXCRETION; NO FECES; VERIFIED AT MULTI-FOCAL NECROPSY: NOT APPLICABLE ADRENAL, CORTEX (AC) : >APPEARANCE; HEAD TILT; LEFT; VERIFIED -ANGIECTASIS,-SLIGHT, MULTI-FOCAL AT NECROPSY: NOT APPLICABLE -DEGENERATION, CYSTIC, CORTICAL,- >EYES; RED DISCHARGE; EYE-RIGHT; MODERATELY SEVERE, FOCAL VERIFIED AT NECROPSY: OBSERVED -HEMORRHAGE,-SLIGHT, MULTI-FOCAL >BEHAVIOR; ATAXIC; VERIFIED AT -PIGMENT,-MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE OVARY (OV) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -MINERALIZATION,-MINIMAL, FOCAL NECROPSY: NOT APPLICABLE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, MULTI-FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2606 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1005 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91187 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 257.8 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 14:45 PROSECTOR: TERRI L. WILLIAMS RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -MASS(ES); 10 MM IN DIAMETER, RED, DARK RED, TAN, VASCULAR AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE -EDEMA,-MINIMAL, DIFFUSE DARK BROWN AREA, 2 X 1 MM STOMACH, NONGL (SU) : -EDEMA,-MODERATE, MULTI-FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 30 X 25 X 25 MM, RED, -B-FIBROADENOMA,-NEOPLASM PRESENT DARK RED, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; LEFT: DARK RED AND TAN (CAP -HEMORRHAGE,-MODERATE, MULTI-FOCAL 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED -ANIMAL THIN -------------------------------------------- 2607 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1006 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91187 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 257.8 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 14:45 PROSECTOR: TERRI L. WILLIAMS RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2608 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1007 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91188 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 570.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:27 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK KIDNEY (KD) : 88; 65 X 70MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MINIMAL, MULTI- OBSERVED FOCAL >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK PANCREAS (PA) : 101; 60 X 55MM; VERIFIED AT NECROPSY: -HEMORRHAGE,-MODERATE, FOCAL OBSERVED ADRENAL, CORTEX (AC) : >MASS(ES); AXILLARY REGION-LEFT; WEEK -ANGIECTASIS,-MODERATELY SEVERE, MULTI- 74; 10 X 10MM; VERIFIED AT NECROPSY: FOCAL OBSERVED -HEMORRHAGE,-MODERATELY SEVERE, MULTI- FOCAL PARATHYROID (PT) : -B-ADENOMA,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE >UNREMARKABLE BLACK AREA, 2 X 1 MM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); PERINEAL REGION: SINGLE -GALACTOCELE,-SEVERE, MULTI-FOCAL MASS, 30 X 21 X 15 MM, TAN, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL BROWN, VASCULAR AND FIRM; ON CUT -NECROSIS,-MODERATELY SEVERE, MULTI- SECTION EXUDES A TAN FLUID; COLLECTED; FOCAL REGIONAL LN (INGUINAL) NORMAL (CAP 20) -B-ADENOMA,-NEOPLASM PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT 2609 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1008 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91188 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 570.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:27 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -CYST(S); TRIMMING COMMENT: ON ROUTINE SECTION: SINGLE TAN, CREAMY SEMISOLID FILLED CYST, 11 X 9 MM; TRIMMED ON ROUTINE SECTION -MASS #2; LEFT LATERAL ABDOMINAL REGION: SINGLE MASS, 70 X 57 X 40 MM, RED, TAN, VASCULAR, AND FIRM; ON CUT SECTION EXUDES A RED FLUID AND A TAN CREAMY SEMIFLUID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NORMAL -THICKENED; ALL REGIONS, DIFFUSE: TAN, BROWN, AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #3; MID VENTRAL CERVICAL EXTENDING TO MID THORACIC REGION: SINGLE MASS, 108 X 70 X 36 MM, RED, TAN, VASCULAR, LOBULATED, AND SOFT; ON SECTION EXUDES A TAN TENACIOUS SEMISOLID; PORTION COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 21) UTERUS (UT) : -DILATATION,-SLIGHT, DIFFUSE VAGINA (VA) : -B-LEIOMYOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2610 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1009 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91188 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 570.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:27 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, INGUINAL (IN) : -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2611 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1010 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91188 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 570.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:27 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), LN, MANDIBULAR (MN), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2612 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1011 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91189 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 313.1 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 12:59 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; LIMBS-HIND; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); AXILLARY REGION-RIGHT; WEEK LIVER (LI) : LIVER (LI) : 88; 30 X 30MM; VERIFIED AT NECROPSY: -DIFFUSELY LIGHT; ALL LOBES: TAN >UNREMARKABLE OBSERVED KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, DIFFUSE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -THROMBUS,-SLIGHT, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 9 X 8 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT 2613 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1012 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91189 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 313.1 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 12:59 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 35 X 31 X 12 MM, RED, TAN, GREY, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE MOIST, TAN ULCERATED AREA, 20 X 19 X 6 MM; COLLECTED WITH MASS CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS UP TO 7 X 5 >UNREMARKABLE X 5 MM (CAP 21) FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT -EDEMA,-MODERATE, FOCAL HINDFOOT: SINGLE MOIST, TAN ABRADED -EROSION,-MODERATELY SEVERE, FOCAL AREA, 17 X 14 MM; COLLECTED -EXOSTOSES,-MODERATELY SEVERE, FOCAL >NOTE:>SORE/SCAB (RIGHT HINDFOOT) NOTED -INFLAMMATION, ACUTE,-MODERATE, MULTI- IN CLINICAL OBSERVATIONS NOT FOCAL SEEN AT NECROPSY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2614 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1013 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91189 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 313.1 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 12:59 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2615 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1014 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91190 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 374.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:37 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); VENTRAL-CERVICAL; WEEK 100; KIDNEY (KD) : KIDNEY (KD) : 50 X 45MM; VERIFIED AT NECROPSY: -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- OBSERVED MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -DIFFUSELY DARK; BILATERAL: DARK BROWN -DEGENERATION, CYSTIC, CORTICAL,- SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -THROMBUS,-MODERATE, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL THYROID (TY) : THYROID (TY) : -LARGE; LEFT: 7 X 4 X 4 MM (RIGHT IS 6 -HEMORRHAGE,-MINIMAL, MULTI-FOCAL X 3 X 2 MM) -B-ADENOMA, C-CELL,-NEOPLASM PRESENT -DIFFUSELY DARK; LEFT: DARK BROWN PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 8 X 8 MM, DARK RED, TAN -HEMORRHAGE,-MODERATE, MULTI-FOCAL AND SOFT -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -INFLAMMATION, ACUTE,-MODERATELY SEVERE, FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL 2616 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1015 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91190 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 374.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:37 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL REGION, -B-FIBROADENOMA,-NEOPLASM PRESENT EXTENDING TO MID THORACIC REGION: SINGLE MASS, 55 X 35 X 20 MM, DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE MANDIBULAR) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN (CAP -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2617 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1016 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91190 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 374.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:37 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2618 Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Author: Peter J. Thomford, PhD Study Completion Date: December 13, 2001 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-212 Sponsor Study Identification: 3M T-6316.1 Volume VII of XII Page 2619 of 4873 Covance 6329-212 3M T-6316.1 VOLUME VII OF XII CONTENTS Page APPENDIX 6 (Continued) ........................................................................................ 2621 Individual Anatomic Pathology Data ..................................................................... 2622 2620 APPENDIX 6 (Continued) Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 2621 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1017 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91191 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/06/99 8:04 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); INGUINAL REGION-RIGHT; WEEK LIVER (LI) : LIVER (LI) : 83; 70 X 70MM; VERIFIED AT NECROPSY: -MOTTLED; ALL LOBES: DARK RED AND TAN -VACUOLATION, HEPATOCELLULAR,- OBSERVED -DEPRESSED FOCUS(I)/AREA(S); LEFT MODERATELY SEVERE, DIFFUSE >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; LATERAL LOBE: MULTIPLE DARK RED AREAS, VERIFIED AT NECROPSY: OBSERVED UP TO 20 X 12 MM; COLLECTED ON ROUTINE >APPEARANCE; SWOLLEN; LIMB-HIND-LEFT; SECTION VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : >MASS(ES); AXILLARY REGION-LEFT; WEEK -MINERALIZATION, TUBULAR,-MINIMAL, 92; 20 X 20MM; VERIFIED AT NECROPSY: MULTI-FOCAL OBSERVED SPLEEN (SP) : -FIBROSIS, CAPSULAR,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL OVARY (OV) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL LUNG (LU) : LUNG (LU) : -DIFFUSELY RED; ALL LOBES: DARK RED >UNREMARKABLE PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 7 X 6 X 4 MM -B-ADENOMA,-NEOPLASM PRESENT 2622 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1018 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91191 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/06/99 8:04 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EROSION,-MODERATE, MULTI-FOCAL MULTIPLE DARK BROWN AREAS, UP TO 2 MM IN DIAMETER MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: TWO -GALACTOCELE,-MINIMAL, MULTI-FOCAL MASSES, EACH IS 18 X 18 X 5 MM, BOTH -MINERALIZATION,-MINIMAL, MULTI-FOCAL ARE TAN AND SOFT; SAME ON BOTH CUT -NECROSIS,-MODERATE, FOCAL SURFACES; BOTH COLLECTED INTACT; -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT REGIONAL LN (SEE AXILLARY) -MASS #2; VENTRAL ABDOMINAL REGION, EXTENDING TO BILATERAL INGUINAL REGIONS: SINGLE MASS, 75 X 65 X 20 MM, TAN, RED, FIRM, LOBULATED AND VASCULAR; SAME ON CUT SURFACES; COLLECTED INTACT; REGIONAL LN (RIGHT INGUINAL) NORMAL (CAP 22) (LEFT INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND SOFT; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -MAMMARY FIBROADENOMA,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -DIFFUSELY RED; RIGHT: DIFFUSELY DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL (CAP 21) FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATELY SEVERE, FOCAL -EXOSTOSES,-MODERATELY SEVERE, FOCAL -FIBROSIS,-MODERATE, FOCAL 2623 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1019 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91191 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/06/99 8:04 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL HINDFOOT: SINGLE DRY BROWN ULCERATED -INFLAMMATION, ACUTE,-SLIGHT, FOCAL AREA, 10 MM IN DIAMETER; SURROUNDING TISSUE APPEARS SWOLLEN; COLLECTED LN, INGUINAL (IN) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, DIFFUSE GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL UPPER INCISORS -------------------------------------------- 2624 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1020 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91191 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/06/99 8:04 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2625 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1021 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91192 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 243.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:51 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0329 .0135 % .0171 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0300 .0123 % .0156 WEIGHT TAKEN BRAIN (BR) 1.9280 .7908 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) .8220 .3372 % .4263 WEIGHT TAKEN RIGHT KIDNEY (KD1) .7920 .3249 % .4108 WEIGHT TAKEN LIVER (LI) 6.3344 2.5982 % 3.2855 WEIGHT TAKEN LUNG (LU) 1.1493 .4714 % .5961 WEIGHT TAKEN LEFT OVARY (OV0) .0567 .0233 % .0294 WEIGHT TAKEN RIGHT OVARY (OV1) .0586 .0240 % .0304 WEIGHT TAKEN SPLEEN (SP) .4607 .1890 % .2390 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0056 .0023 % .0029 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0043 .0018 % .0022 WEIGHT TAKEN UTERUS (UT) .5438 .2231 % .2821 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -RED FOCUS(I)/AREA(S); BILATERAL: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL MULTIPLE DARK RED PINPOINT FOCI (CAP 20) GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 2626 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1022 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91192 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 243.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:51 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2627 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1023 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91193 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 306.8 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 14:23 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); AXILLARY REGION-LEFT; WEEK -DILATATION, VENTRICULAR,-PRESENT 85; 25 X 20MM; VERIFIED AT NECROPSY: -HEMORRHAGE,-MODERATE, FOCAL OBSERVED ADRENAL, CORTEX (AC) : >EXCRETION; FEW FECES; VERIFIED AT -ANGIECTASIS,-SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE -HEMORRHAGE,-SLIGHT, MULTI-FOCAL >DISCHARGE; NASAL; RED IN COLOR; THYMUS (TH) : VERIFIED AT NECROPSY: OBSERVED -CYST,-PRESENT >SKIN & PELAGE; RED HAIR COAT; PERINEAL PARATHYROID (PT) : AREA; VERIFIED AT NECROPSY: OBSERVED >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL GRAY AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 40 X 30 X 18 MM, RED, TAN, LOBULATED AND FIRM; SOME SECTIONS EXUDE A YELLOW CREAMY SEMIFLUID MATERIAL; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) CERVIX (CV) : CERVIX (CV) : -LARGE; UP TO 7 MM IN DIAMETER >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2628 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1024 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91193 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 306.8 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 14:23 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); RIGHT HIND -CYST, EPIDERMAL INCLUSION,-PRESENT FOOT: SINGLE DARK RED ABRADED AREA, 3 -FIBROSIS,-MODERATE, FOCAL MM IN DIAMETER; COLLECTED -INFLAMMATION, ACUTE,-SLIGHT, FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; RED AND YELLOW MOIST MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -DISCHARGE-NASAL; DRY RED MATERIAL -------------------------------------------- 2629 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1025 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91193 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 306.8 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 14:23 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2630 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1026 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91194 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/23/00 STUDY DAY OF DEATH: 728 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 356.1 GRAMS DATE AND TIME OF NECROPSY: 01/23/00 14:31 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 71; 65 X 60MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; MALOCCLUSION; VERIFIED AT -HEMORRHAGE,-MODERATE, FOCAL NECROPSY: NOT OBSERVED LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NECROPSY: OBSERVED FOCAL >EXCRETION; FEW FECES; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MINIMAL, MULTI- >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED FOCAL AT NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >RESPIRATION; LABORED; VERIFIED AT MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE, FOCAL -VACUOLATION,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 4 MM, RED, TAN -HEMORRHAGE,-MODERATE, MULTI-FOCAL AND SOFT -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,-NEOPLASM PRESENT 2631 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1027 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91194 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/23/00 STUDY DAY OF DEATH: 728 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 356.1 GRAMS DATE AND TIME OF NECROPSY: 01/23/00 14:31 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 61 X 52 X 20 MM, RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 29 X 20 X 19 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 21) -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 2632 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1028 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91194 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/23/00 STUDY DAY OF DEATH: 728 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 356.1 GRAMS DATE AND TIME OF NECROPSY: 01/23/00 14:31 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2633 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1029 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91195 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 366.3 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 13:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 87; 45 X 35MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; HEAD TILT; RIGHT; VERIFIED LIVER (LI) : LIVER (LI) : AT NECROPSY: NOT APPLICABLE -RED FOCUS(I)/AREA(S); ALL LOBES: >UNREMARKABLE >EXCRETION; FEW FECES; VERIFIED AT MULTIPLE DARK RED AREAS, UP TO 6 MM IN NECROPSY: NOT APPLICABLE DIAMETER; COLLECTED ON ROUTINE >APPEARANCE; THIN; VERIFIED AT SECTIONS NECROPSY: OBSERVED KIDNEY (KD) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE MODERATE, MULTI-FOCAL SPLEEN (SP) : -B-HEMANGIOMA,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- MINIMAL, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 7 MM, DARK RED, -HEMORRHAGE,-MINIMAL, MULTI-FOCAL RED, TAN, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT 2634 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1030 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91195 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 366.3 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 13:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, NONGL (SU) : -EDEMA,-SLIGHT, MULTI-FOCAL -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI- FOCAL JEJUNUM (JE) : JEJUNUM (JE) : -DIVERTICULUM(A); TRIMMING COMMENT: -DIVERTICULUM,-PRESENT MID JEJUMUM: SINGLE AREA, 6 X 5 X 5 MM; COLLECTED ON ROUTINE SECTION MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 40 X 35 X 20 MM, TAN, GRAY, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) UTERUS (UT) : UTERUS (UT) : -CYST(S); LEFT, WITHIN WALL: SINGLE >UNREMARKABLE CLEAR FLUID FILLED CYST, 4 X 3 X 3 MM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2635 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1031 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91195 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 366.3 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 13:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2636 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1032 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91196 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 360.3 GRAMS DATE AND TIME OF NECROPSY: 08/27/99 8:04 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 80; 50 X 30MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; NO FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >SKIN & PELAGE; BROWN HAIR COAT; FOCAL VENTRAL-ABDOMINAL; VERIFIED AT -VACUOLATION, HEPATOCELLULAR,- NECROPSY: NOT OBSERVED MODERATELY SEVERE, MULTI-FOCAL >SKIN & PELAGE; RED HAIR COAT; HEAD- KIDNEY (KD) : ENTIRE; VERIFIED AT NECROPSY: NOT -PYELONEPHRITIS,-SLIGHT, MULTI-FOCAL OBSERVED ADRENAL, CORTEX (AC) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -ANGIECTASIS,-SLIGHT, MULTI-FOCAL NECROPSY: OBSERVED -HEMORRHAGE,-SLIGHT, MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT HEART (HT) : NECROPSY: NOT APPLICABLE -DEGENERATION/NECROSIS,-MINIMAL, MULTI- >SKIN & PELAGE; SORE/SCAB; PAW-HIND- FOCAL LEFT; VERIFIED AT NECROPSY: OBSERVED PITUITARY (PI) : PITUITARY (PI) : >APPEARANCE; THIN; VERIFIED AT -MASS(ES); SINGLE MASS, 12 X 9 X 9 MM, -HEMORRHAGE,-SEVERE, DIFFUSE NECROPSY: NOT OBSERVED RED, DARK RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT >BEHAVIOR; ATAXIC; VERIFIED AT MAMMARY, FEMALE (MF) : NECROPSY: NOT APPLICABLE -GALACTOCELE,-MINIMAL, MULTI-FOCAL >SKIN & PELAGE; PALE; EARS; VERIFIED AT -B-FIBROADENOMA,-NEOPLASM PRESENT NECROPSY: NOT APPLICABLE 2637 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1033 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91196 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 360.3 GRAMS DATE AND TIME OF NECROPSY: 08/27/99 8:04 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 38 X 26 X 23 MM, RED, DARK RED, TAN, FIRM, AND LOBULATED; SOME SECTIONS EXUDE A BROWN FLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT -EDEMA,-MODERATE, MULTI-FOCAL HINDFOOT: SINGLE TAN AND DRY ABRADED -EROSION,-MODERATE, FOCAL AREA, 6 X 5 MM, COLLECTED -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -------------------------------------------- 2638 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1034 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91196 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 360.3 GRAMS DATE AND TIME OF NECROPSY: 08/27/99 8:04 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2639 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1035 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91197 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 583.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:14 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 10 MM IN DIAMETER (LEFT -ANGIECTASIS,-MODERATE, MULTI-FOCAL IS 7 X 4 X 3 MM); APPEARS CYSTIC -CYST,-SEVERE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -THROMBUS,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); RIGHT ASPECT: -B-ADENOMA,-NEOPLASM PRESENT SINGLE DARK RED AREA, 4 X 2 MM JEJUNUM (JE) : JEJUNUM (JE) : -LARGE LYMPHOID AREA(S); DIFFUSE: UP -HYPERPLASIA, GALT (GUT ASSOCIATED TO 7 X 4 X 3 MM; COLLECTED ON ROUTINE LYMPHOID TISSUE),-MINIMAL, MULTI-FOCAL SECTION 2640 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1036 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91197 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 583.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:14 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ILEUM (IL) : ILEUM (IL) : -LARGE LYMPHOID AREA(S); DIFFUSE: UP -HYPERPLASIA, GALT (GUT ASSOCIATED TO 7 X 4 X 3 MM; COLLECTED ON ROUTINE LYMPHOID TISSUE),-MINIMAL, MULTI-FOCAL SECTION MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL REGION: -B-ADENOMA,-NEOPLASM PRESENT SINGLE MASS, 7 MM IN DIAMETER, RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (MANDIBULAR) NORMAL (CAP 21) -THICKENED; LEFT INGUINAL REGION: TAN, DARK BROWN AND SOFT; EXUDES A DARK BROWN FLUID; COLLECTED ON ROUTINE SECTION UTERUS (UT) : UTERUS (UT) : -CYST(S); RIGHT, WITHIN LUMEN: SINGLE -DILATATION,-MODERATELY SEVERE, FOCAL CLEAR FLUID FILLED CYST, 6 X 3 X 3 MM -B-POLYP, ENDOMETRIAL, STROMAL,- -MASS(ES); DISTAL END OF RIGHT HORN, NEOPLASM PRESENT WITHIN LUMEN: SINGLE MASS, 22 X 5 X 5 MM, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 22) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOCYTIC,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 2641 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1037 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91197 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 583.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:14 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LUNG (LU), URINARY BLADDER (UB), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 2642 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1038 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91198 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 231.2 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:11 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.5213 3.2532 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2643 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1039 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91198 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 231.2 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:11 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2644 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1040 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91199 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 371.8 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:48 PROSECTOR: JERALD W. JOHNSON RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; YELLOW HAIR COAT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >APPEARANCE; HEAD TILT; RIGHT; VERIFIED -RED FOCUS(I)/AREA(S); ALL LOBES: -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- AT NECROPSY: OBSERVED MULTIPLE DARK RED AREAS, UP TO 7 MM IN FOCAL >BEHAVIOR; ATAXIC; VERIFIED AT DIAMETER; SOME APPEAR SUNKEN WITH A -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE STELLATE PATTERN; COLLECTED ON ROUTINE FOCAL >BEHAVIOR; CIRCLING; LEFT; VERIFIED AT SECTIONS NECROPSY: NOT APPLICABLE KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI- FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 7 MM IN DIAMETER (LEFT -ANGIECTASIS,-SLIGHT, MULTI-FOCAL IS 5 MM IN DIAMETER) -DEGENERATION, CYSTIC, CORTICAL,- -RED FOCUS(I)/AREA(S); RIGHT: SINGLE SEVERE, FOCAL DARK RED AREA, 6 MM IN DIAMETER -HEMORRHAGE,-MODERATE, MULTI-FOCAL -THROMBUS,-SLIGHT, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 2645 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1041 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91199 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 371.8 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:48 PROSECTOR: JERALD W. JOHNSON RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 9 X 7 MM, RED, DARK -HEMORRHAGE,-MODERATELY SEVERE, MULTI- RED, TAN, GRAY AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: -GALACTOCELE,-MODERATE, MULTI-FOCAL MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS (CAP 21) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2646 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1042 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91199 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 371.8 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:48 PROSECTOR: JERALD W. JOHNSON RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2647 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1043 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91200 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/24/99 STUDY DAY OF DEATH: 607 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 445.0 GRAMS DATE AND TIME OF NECROPSY: 09/24/99 13:57 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); HEAD; WEEK 82; 30 X 35MM; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, TUBULAR,-MINIMAL, >SKIN & PELAGE; SORE/SCAB; HEAD- FOCAL CRANIAL; VERIFIED AT NECROPSY: ADRENAL, CORTEX (AC) : OBSERVED -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : SKIN (SK) : -MASS(ES); TOP OF HEAD, EXTENDS TO -M-FIBROSARCOMA,-NEOPLASM PRESENT BOTH AUDITORY CANALS AND ORBITS: SINGLE SUBCUTANEOUS MASS, 23 X 35 X 52 MM, FIRM, RED, TAN AND SMOOTH; SAME ON CUT SURFACE; OVERLYING SKIN HAS A DARK BROWN, DRY CRUSTED AREA, 10 X 20 MM (COLLECTED INTACT WITH MASS); REGIONAL LN (MANDIBULAR) NORMAL (COLLECTED) 2648 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1044 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91200 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/24/99 STUDY DAY OF DEATH: 607 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 445.0 GRAMS DATE AND TIME OF NECROPSY: 09/24/99 13:57 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : -DILATATION,-SLIGHT, FOCAL CAUSE OF DEATH (CD) : -FIBROSARCOMA,-PRESENT CALVARIUM/SKULL (CA) : CALVARIUM/SKULL (CA) : -DEPRESSED AREA(S); ENTIRE SKULL CAP >UNREMARKABLE UNDER SKIN MASS (COLLECTED) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2649 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1045 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91200 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/24/99 STUDY DAY OF DEATH: 607 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 445.0 GRAMS DATE AND TIME OF NECROPSY: 09/24/99 13:57 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2650 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1046 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91201 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 374.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:46 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 74; 60 X 45MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); INGUINAL REGION-RIGHT; WEEK LIVER (LI) : LIVER (LI) : 97; 50 X 30MM; VERIFIED AT NECROPSY: -MASS(ES); ATTACHED TO MARGIN OF LEFT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL OBSERVED LATERAL LOBE: SINGLE MASS, 15 X 15 X 4 -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM >EYES; RED DISCHARGE; EYES; VERIFIED AT MM, DARK RED, BROWN AND FIRM; SAME ON PRESENT NECROPSY: OBSERVED CUT SURFACE (CAP 20); REGIONAL LN >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; (HEPATIC) NOT IDENTIFIED WEEK 105; 20 X 20MM; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 5 MM, RED, TAN AND -B-ADENOMA,-NEOPLASM PRESENT FIRM MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT 2651 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1047 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91201 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 374.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:46 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 65 X 45 X 43 MM, RED, DARK RED, TAN, FIRM AND LOBULATED; SOME SECTIONS EXUDE A TAN, CREAMY MATERIAL; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) -MASS #2; VENTRAL LUMBAR REGION: SINGLE MASS, 55 X 35 X 28 MM, DARK BROWN, TAN AND CYSTIC; ON SECTION EXUDES A DARK BROWN FLUID; COLLECTED; POSSIBLY CONSISTENT WITH MASSES (INGUINAL REGION, RIGHT AND VENTRAL ABDOMINAL REGION, RIGHT) AS NOTED IN CLINICAL OBSERVATIONS; REGIONAL LN (LUMBAR) NORMAL (CAP 22) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -BONE MARROW SMEAR TAKEN -------------------------------------------- 2652 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1048 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91201 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 374.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:46 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), URINARY BLADDER (UB), LN, LUMBAR/ILIAC (LM), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2653 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1049 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91202 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 619.0 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 14:40 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK KIDNEY (KD) : 76; 40 X40MM; VERIFIED AT NECROPSY: -CAST, PROTEINACEOUS,-MINIMAL, MULTI- OBSERVED FOCAL >MASS(ES); VENTRAL-THORACIC-RIGHT; WEEK ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : 84; 10 X 15MM; VERIFIED AT NECROPSY: -LARGE; LEFT: 9 X 5 X 5 MM (RIGHT IS 6 -DEGENERATION, CYSTIC, CORTICAL,- OBSERVED X 4 X 3 MM) SEVERE, FOCAL >SKIN & PELAGE; BLACK SKIN; VENTRAL- -HEMORRHAGE,-MODERATELY SEVERE, MULTI- THORACIC-RIGHT; VERIFIED AT NECROPSY: FOCAL OBSERVED ADRENAL, MEDULLA (MA) : >MASS(ES); INGUINAL REGION-LEFT; WEEK -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT 84; 20 X 10MM; VERIFIED AT NECROPSY: LUNG (LU) : OBSERVED -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI- FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL 2654 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1050 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91202 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 619.0 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 14:40 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : -ULCERATION,-SEVERE, MULTI-FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY AND RIGHT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT VENTRAL THORACIC REGIONS: TWO MASSES, RIGHT AXILLARY IS 50 X 34 X 23 MM, RIGHT THORACIC IS 23 X 23 X 10 MM; BOTH ARE TAN, RED, DARK RED, FIRM, AND LOBULATED; SAME ON CUT SURFACES; OVERLYING SKIN ON THE THORACIC REGION MASS HAS A SINGLE DARK RED AND DRY CRUSTED AREA, 19 MM IN DIAMETER; MASS WITH CRUSTED AREA COLLECTED; REGIONAL LN (SEE AXILLARY) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE WHITE CREAMY FLUID FILLED CYSTS, UP TO 10 MM IN DIAMETER (CAP 22) -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 27 X 19 X 14 MM, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -MAMMARY FIBROADENOMA,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; RIGHT: DARK RED AND TAN -HEMORRHAGE,-SLIGHT, MULTI-FOCAL (LEFT NOT IDENTIFIED) (CAP 21) 2655 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1051 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91202 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 619.0 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 14:40 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2656 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1052 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91203 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/11/99 STUDY DAY OF DEATH: 532 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/11/99 14:16 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 60; 40 X 40MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL AT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED -LARGE; ALL LOBES; MARGINS APPEAR >UNREMARKABLE AT NECROPSY: NOT APPLICABLE ROUNDED >SKIN & PELAGE; PALE; ENTIRE BODY; KIDNEY (KD) : VERIFIED AT NECROPSY: NOT APPLICABLE -CAST, PROTEINACEOUS,-SLIGHT, MULTI- >RESPIRATION; IRREGULAR; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-SLIGHT, MULTI- FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -DIFFUSELY RED; BILATERAL: DARK RED -ANGIECTASIS,-MODERATELY SEVERE, MULTI- -LARGE; BILATERAL: EACH IS 6 MM IN FOCAL DIAMETER -HEMORRHAGE,-MODERATELY SEVERE, MULTI- FOCAL OVARY (OV) : -CYST,-PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 5 X 5 MM, DARK RED AND -HEMORRHAGE,-MODERATELY SEVERE, MULTI- SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2657 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1053 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91203 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/11/99 STUDY DAY OF DEATH: 532 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/11/99 14:16 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE -EDEMA,-MODERATE, MULTI-FOCAL TAN, ERODED AREA, 2 MM IN DIAMETER MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 40 X 35 X 35 MM, TAN, RED, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A TAN, CREAMY SEMIFLUID MATERIAL; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) -THICKENED; ALL REGIONS: DIFFUSE; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2658 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1054 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91203 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/11/99 STUDY DAY OF DEATH: 532 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/11/99 14:16 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2659 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1055 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91204 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/27/99 STUDY DAY OF DEATH: 426 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/27/99 8:29 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL; WEEK 35; EYE (EY) : 45 X 35MM; VERIFIED AT NECROPSY: -MINERALIZATION, CORNEAL,-MODERATE, OBSERVED FOCAL >SKIN & PELAGE; SORE/SCAB; VENTRAL- BRAIN (BR) : BRAIN (BR) : ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : -FIBROSIS,-MINIMAL, FOCAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-SLIGHT, MULTI- FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 7 X 5 X 4 MM; APPEARS SWOLLEN -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL ABDOMINAL REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 65 X 55 X 18 MM, RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL DARK RED, BLACK, TAN, GRAY, LOBULATED -MINERALIZATION,-MODERATE, MULTI-FOCAL AND FIRM; SAME ON CUT SURFACE; -M-CARCINOMA,-NEOPLASM PRESENT COLLECTED INTACT; REGIONAL LN (SEE INGUINAL); OVERLYING SKIN HAS A SINGLE BLACK AND MOIST ULCERATED AREA, 15 MM IN DIAMETER; COLLECTED WITH MASS 2660 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1056 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91204 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/27/99 STUDY DAY OF DEATH: 426 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/27/99 8:29 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, INGUINAL (IN) : LN, INGUINAL (IN) : -LARGE; LEFT: 15 X 13 X 10 MM (FOR >UNREMARKABLE COMPARISON, RIGHT IS 4 X 2 X 2 MM) (CAP 20) -DIFFUSELY RED; LEFT: DARK RED (CAP 20) GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: CLEAR MOIST MATERIAL -------------------------------------------- 2661 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1057 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91204 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/27/99 STUDY DAY OF DEATH: 426 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/27/99 8:29 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2662 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1058 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91205 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 167.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:19 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 5.6721 3.3944 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2663 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1059 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91205 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 167.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:19 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2664 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1060 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91206 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 336.8 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:05 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >SKIN & PELAGE; BROWN HAIR COAT; -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- PERINEAL AREA; VERIFIED AT NECROPSY: FOCAL NOT OBSERVED KIDNEY (KD) : >EYES; PALE; EYES; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 8 X 6 MM, -CYST,-PRESENT DARK RED, TAN, GRAY, LOBULATED AND -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL VAGINA (VA) : -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT 2665 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1061 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91206 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 336.8 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:05 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS UP TO 12 X -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, 8 X 6 MM; COLLECTED DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER AND LOWER INCISORS -------------------------------------------- 2666 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1062 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91206 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 336.8 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:05 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2667 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1063 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91207 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 417.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:34 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT OVARY (OV) : OVARY (OV) : NECROPSY: OBSERVED -CYST(S); RIGHT: MULTIPLE CLEAR FLUID -CYST,-PRESENT FILLED CYSTS, UP TO 5 MM IN DIAMETER LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); MID ASPECT: -HEMORRHAGE,-MINIMAL, MULTI-FOCAL SINGLE DARK RED AREA, 2 MM IN DIAMETER -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; -HEMORRHAGE,-MINIMAL, MULTI-FOCAL COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; LEFT UPPER INCISOR -BONE MARROW SMEAR TAKEN -------------------------------------------- 2668 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1064 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91207 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 417.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:34 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), KIDNEY (KD), PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2669 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1065 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91208 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 259.8 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:30 PROSECTOR: JODI THIEREN RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: OBSERVED KIDNEY (KD) : >APPEARANCE; HUNCHED; VERIFIED AT -MINERALIZATION, PELVIC,-MODERATE, NECROPSY: NOT APPLICABLE MULTI-FOCAL >SKIN & PELAGE; YELLOW HAIR COAT; ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : VERIFIED AT NECROPSY: NOT OBSERVED -LIGHT FOCUS(I)/AREA(S); BILATERAL: -ANGIECTASIS,-MODERATE, MULTI-FOCAL >EXCRETION; FEW FECES; VERIFIED AT MULTIPLE TAN PINPOINT FOCI -DEGENERATION, CYSTIC, CORTICAL,- NECROPSY: NOT APPLICABLE MODERATE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 5 MM IN DIAMETER PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 9 X 9 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- TAN, LOBULATED AND FIRM FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT CAVITY, ABDOM (PC) : -NECROSIS, FAT,-PRESENT 2670 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1066 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91208 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 259.8 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:30 PROSECTOR: JODI THIEREN RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE, NEAR RIGHT OVARY: SINGLE MASS, 6 X 5 X 5 MM, TAN, YELLOW, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (RENAL) NORMAL (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY MATERIAL -ANIMAL THIN -------------------------------------------- 2671 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1067 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91208 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 259.8 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:30 PROSECTOR: JODI THIEREN RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, RENAL (RL) ------------------------------------------------------------------------------------------------------------------------------------ 2672 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1068 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91209 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/29/99 STUDY DAY OF DEATH: 520 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/29/99 15:27 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -DIFFUSELY RED; BILATERAL: DARK RED -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID >UNREMARKABLE FILLED CYST, 3 MM IN DIAMETER THYMUS (TH) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 MM IN DIAMETER, DARK RED -HEMORRHAGE,-MODERATELY SEVERE, MULTI- AND SOFT FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT ILEUM (IL) : >AUTOLYTIC MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS: DIFFUSE; -GALACTOCELE,-SLIGHT, MULTI-FOCAL COLLECTED ON ROUTINE SECTION 2673 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1069 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91209 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/29/99 STUDY DAY OF DEATH: 520 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/29/99 15:27 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2674 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1070 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91210 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/02/99 STUDY DAY OF DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/02/99 9:11 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; HEAD- BRAIN (BR) : BRAIN (BR) : CRANIAL; VERIFIED AT NECROPSY: NOT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -RED FOCUS(I)/AREA(S); ALL LOBES: -FOCUS, ALTERED HEPATOCELLULAR, NECROPSY: NOT APPLICABLE MULTIPLE DARK RED AREAS, UP TO 5 MM IN EOSINOPHILIC,-MODERATE, FOCAL >SKIN & PELAGE; COLD TO TOUCH; ENTIRE DIAMETER; COLLECTED ON ROUTINE -TELANGIECTASIS,-MODERATE, FOCAL BODY; VERIFIED AT NECROPSY: NOT SECTIONS APPLICABLE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 7 MM, DARK RED, -HEMORRHAGE,-SEVERE, MULTI-FOCAL GRAY AND SOFT -B-ADENOMA,-NEOPLASM PRESENT 2675 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1071 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91210 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/02/99 STUDY DAY OF DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/02/99 9:11 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); DIFFUSE: MULTIPLE TAN AND -GALACTOCELE,-MODERATE, MULTI-FOCAL CREAMY FLUID FILLED CYSTS, UP TO 4 MM -MINERALIZATION,-MINIMAL, MULTI-FOCAL IN DIAMETER; COLLECTED ON ROUTINE SECTION UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -DILATATION,-MODERATELY SEVERE, DIFFUSE MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 7 X 5 X 5 MM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL; COLLECTED >UNREMARKABLE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY MATERIAL -------------------------------------------- 2676 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1072 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91210 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/02/99 STUDY DAY OF DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/02/99 9:11 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2677 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1073 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91211 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 562.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:04 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 86; 20 X 40MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN -DEGENERATION, CYSTIC,-SLIGHT, FOCAL -RED FOCUS(I)/AREA(S); LEFT AND RIGHT LATERAL LOBES: MULTIPLE RED AREAS UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI- FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : >NOTE:>LEFT ADRENAL DAMAGED AT NECROPSY >NOT REQUIRED TO BE EXAMINED FOR ANIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 5 MM, DARK RED, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL GRAY AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT 2678 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1074 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91211 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 562.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:04 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 36 X 24 X 20 MM, DARK RED, RED, GRAY, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN OF MASS HAS A BLACK CRUSTED AREA, 10 MM IN DIAMETER; COLLECTED INTACT WITH MASS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -ANIMAL OBESE GENERAL INFORMATION (XX) : >NOTE:>NECROPSIED OUT OF ORDER DUE TO MORIBUND CONDITION FOLLOWING BLOOD COLLECTION -------------------------------------------- 2679 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1075 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91211 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 562.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:04 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), THYROID (TY), SKIN (SK), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2680 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1076 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91212 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/07/99 STUDY DAY OF DEATH: 467 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/07/99 13:20 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : 49; 10 X 10MM; VERIFIED AT NECROPSY: -HEMORRHAGE,-MINIMAL, MULTI-FOCAL NOT OBSERVED LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -VACUOLATION, HEPATOCELLULAR,-MINIMAL, NECROPSY: NOT OBSERVED MULTI-FOCAL >APPEARANCE; HEAD TILT; RIGHT; VERIFIED KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 6 MM IN -DEGENERATION, CYSTIC, CORTICAL,- DIAMETER SEVERE, FOCAL -HEMORRHAGE,-SEVERE, FOCAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SINGLE DARK RED AREA, 3 X 2 MM -B-ADENOMA,-NEOPLASM PRESENT -LARGE; 6 X 6 X 3 MM; APPEARS SWOLLEN MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -PIGMENT,-MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT -------------------------------------------- 2681 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1077 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91212 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/07/99 STUDY DAY OF DEATH: 467 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/07/99 13:20 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2682 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1078 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91213 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/14/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 339.7 GRAMS DATE AND TIME OF NECROPSY: 07/14/99 16:14 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; HEAD- BRAIN (BR) : BRAIN (BR) : ENTIRE; VERIFIED AT NECROPSY: NOT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -FIBROSIS,-MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >EYES; RED DISCHARGE; EYES; VERIFIED AT FOCAL NECROPSY: OBSERVED -NECROSIS, HEPATOCELLULAR,-MINIMAL, >APPEARANCE; HEAD TILT; RIGHT; VERIFIED MULTI-FOCAL AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >APPEARANCE; THIN; VERIFIED AT -MINERALIZATION, PELVIC,-SLIGHT, MULTI- NECROPSY: OBSERVED FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 11 X 10 X 10 MM (FOR -ANGIECTASIS,-MODERATE, MULTI-FOCAL COMPARISON, RIGHT IS 6 X 5 X 4 MM) -DEGENERATION, CYSTIC, CORTICAL,- MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL LUNG (LU) : -MINERALIZATION,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 10 X 7 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT VAGINA (VA) : >UNREMARKABLE 2683 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1079 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91213 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/14/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 339.7 GRAMS DATE AND TIME OF NECROPSY: 07/14/99 16:14 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN AND CREAMY SEMIFLUID CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -------------------------------------------- 2684 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1080 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91213 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/14/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 339.7 GRAMS DATE AND TIME OF NECROPSY: 07/14/99 16:14 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2685 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1081 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91214 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 687 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 557.1 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 15:17 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 64; 50 X 90MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >MASS(ES); VENTRAL-CERVICAL; WEEK 95; -FOCUS, ALTERED HEPATOCELLULAR, 85 X 90MM; VERIFIED AT NECROPSY: EOSINOPHILIC,-SLIGHT, MULTI-FOCAL OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- >MASS(ES); INGUINAL REGION-RIGHT; WEEK FOCAL 84; 10 X 10MM; VERIFIED AT NECROPSY: -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- OBSERVED FOCAL >EYES; RED DISCHARGE; EYE-LEFT; -TELANGIECTASIS,-MINIMAL, MULTI-FOCAL VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : >APPEARANCE; THIN; VERIFIED AT -MINERALIZATION, PELVIC,-MINIMAL, FOCAL NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -SMALL; LEFT: 3 MM IN DIAMETER (RIGHT -ANGIECTASIS,-MODERATE, MULTI-FOCAL IS 5 MM IN DIAMETER) -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, FOCAL -THROMBUS,-MINIMAL, FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL THYROID (TY) : -HEMORRHAGE,-MINIMAL, FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 2686 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1082 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91214 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 687 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 557.1 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 15:17 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); RIGHT POSTERIOR ASPECT: -HEMORRHAGE,-MINIMAL, MULTI-FOCAL SINGLE MASS, 4 X 3 X 2 MM, RED, GREY, -B-ADENOMA,-NEOPLASM PRESENT BROWN AND SOFT -LARGE; 7 X 5 X 5 MM; APPEARS SWOLLEN HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MODERATE, MULTI- FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -B-FIBROADENOMA,- 3 NEOPLASMS PRESENT SINGLE MASS, 19 X 18 X 11 MM, GREY, TAN, AND SOFT; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 91 X 53 X 35 MM, RED, DARK RED, GREY, TAN, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH MASS (VENTRAL-LEFT- ABDOMINAL REGION) AS NOTED IN CLINICAL OBSERVATIONS -MASS #3; INVOLVING ENTIRE VENTRAL CERVICAL AND THORACIC REGIONS: SINGLE MASS, 101 X 97 X 58 MM (141.7 GRAMS), RED, DARK RED, GREY, TAN, LOBULATED, VASCULAR AND SOFT; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE MANDIBULAR) 2687 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1083 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91214 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 687 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 557.1 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 15:17 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MULTIPLE CLEAR FLUID FILLED CYSTS, UP MODERATE, MULTI-FOCAL TO 5 MM IN DIAMETER CAUSE OF DEATH (CD) : -MAMMARY FIBROADENOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS UP TO 6 X 5 >TISSUE MISSING X 3 MM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DRY, RED MATERIAL -ANIMAL THIN -------------------------------------------- 2688 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1084 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91214 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 687 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 557.1 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 15:17 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2689 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1085 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91215 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/07/99 STUDY DAY OF DEATH: 378 STUDY WEEK OF DEATH: 54 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/07/99 12:55 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >HOUSING,FOOD,WATER & MAINT.; TEETH -DILATATION, VENTRICULAR,-PRESENT CUT; VERIFIED AT NECROPSY: NOT -HEMORRHAGE,-MODERATE, MULTI-FOCAL APPLICABLE LIVER (LI) : >SKIN & PELAGE; COLD TO TOUCH; LIMBS- -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, HIND; VERIFIED AT NECROPSY: NOT MULTI-FOCAL APPLICABLE KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 15 X 12 MM, DARK RED, -HEMORRHAGE,-SEVERE, MULTI-FOCAL TAN, AND SOFT -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL 2690 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1086 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91215 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/07/99 STUDY DAY OF DEATH: 378 STUDY WEEK OF DEATH: 54 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/07/99 12:55 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -DILATATION,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -------------------------------------------- 2691 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1087 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91215 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/07/99 STUDY DAY OF DEATH: 378 STUDY WEEK OF DEATH: 54 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/07/99 12:55 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2692 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1088 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91216 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 365.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:28 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : NECROPSY: NOT OBSERVED -CYST, BILIARY,-MODERATE, FOCAL >MASS(ES); INGUINAL REGION-RIGHT; WEEK -TELANGIECTASIS,-MINIMAL, FOCAL 98; 50 X 30MM; VERIFIED AT NECROPSY: -VACUOLATION, HEPATOCELLULAR,-SLIGHT, OBSERVED MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -MASS(ES); INVOLVING ENTIRE LEFT -DEGENERATION, CYSTIC, CORTICAL,- GLAND: SINGLE MASS, 18 X 13 X 10 MM, MODERATELY SEVERE, MULTI-FOCAL DARK RED, TAN AND LOBULATED; ON -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SECTION, EXUDES A TAN CASEOUS MATERIAL; REGIONAL LN (SEE RENAL) ADRENAL, MEDULLA (MA) : -M-MALIGNANT PHEOCHROMOCYTOMA,-NEOPLASM PRESENT OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: MULTIPLE CLEAR -CYST,-PRESENT FLUID FILLED CYSTS ON EACH, EACH IS UP TO 5 MM IN DIAMETER 2693 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1089 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91216 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 365.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:28 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -N-MALIGNANT PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); MID ANTERIOR -HEMORRHAGE,-MODERATELY SEVERE, FOCAL ASPECT: SINGLE DARK RED AREA, 3 MM IN -B-ADENOMA,-NEOPLASM PRESENT DIAMETER -LARGE; 6 X 5 X 4 MM; APPEARS SWOLLEN MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -GALACTOCELE,-MINIMAL, MULTI-FOCAL SINGLE MASS, 30 X 25 X 20 MM, TAN, -B-FIBROADENOMA,-NEOPLASM PRESENT FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 23) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; RIGHT: 7 X 4 X 2 MM (LEFT IS 3 -HEMORRHAGE,-SLIGHT, MULTI-FOCAL X 2 X 1 MM) (CAP 22) -DIFFUSELY RED; RIGHT: DARK RED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2694 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1090 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91216 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 365.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:28 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), URINARY BLADDER (UB), LN, INGUINAL (IN) ------------------------------------------------------------------------------------------------------------------------------------ 2695 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1091 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91217 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/12/99 STUDY DAY OF DEATH: 472 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 271.5 GRAMS DATE AND TIME OF NECROPSY: 05/12/99 13:45 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; BROWN HAIR COAT; BRAIN (BR) : BRAIN (BR) : VENTRAL-ABDOMINAL; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; NO FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR,-MODERATE, >APPEARANCE; THIN; VERIFIED AT MULTI-FOCAL NECROPSY: OBSERVED KIDNEY (KD) : KIDNEY (KD) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -LARGE PELVIS(ES); TRIMMING COMMENT: -CAST, PROTEINACEOUS,-MODERATE, FOCAL NECROPSY: OBSERVED RIGHT -DILATATION, PELVIC,-PRESENT >RESPIRATION; IRREGULAR; VERIFIED AT -HEMORRHAGE,-SLIGHT, FOCAL NECROPSY: NOT APPLICABLE -HYPERPLASIA, TRANSITIONAL CELL,- MINIMAL, MULTI-FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); TRIMMING COMMENT: RIGHT: -CYST,-PRESENT SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 2696 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1092 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91217 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/12/99 STUDY DAY OF DEATH: 472 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 271.5 GRAMS DATE AND TIME OF NECROPSY: 05/12/99 13:45 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, AND SOFT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BROWN AND DRY -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND CRUSTED -ANIMAL THIN -------------------------------------------- 2697 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1093 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91217 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/12/99 STUDY DAY OF DEATH: 472 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 271.5 GRAMS DATE AND TIME OF NECROPSY: 05/12/99 13:45 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2698 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1094 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91218 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 329.4 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:41 PROSECTOR: JODI THIEREN RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HUNCHED; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; HEAD TILT; LEFT; VERIFIED -DILATATION, VENTRICULAR,-PRESENT AT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -DIFFUSELY LIGHT; PAPILLARY PROCESS: -FOCUS, ALTERED HEPATOCELLULAR, NECROPSY: OBSERVED TAN (CAP 21) EOSINOPHILIC,-SLIGHT, FOCAL >EXCRETION; FEW FECES; VERIFIED AT -HEMORRHAGE,-MODERATE, MULTI-FOCAL NECROPSY: NOT APPLICABLE -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- >BEHAVIOR; ATAXIC; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE -NECROSIS, HEPATOCELLULAR,-MODERATE, >DISCHARGE; NASAL; RED IN COLOR; MULTI-FOCAL VERIFIED AT NECROPSY: NOT OBSERVED -TELANGIECTASIS,-MINIMAL, FOCAL KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT 2699 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1095 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91218 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 329.4 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:41 PROSECTOR: JODI THIEREN RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 15 X 10 X 10 -HEMORRHAGE,-SEVERE, DIFFUSE MM, DARK RED, GRAY AND FIRM -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE >UNREMARKABLE STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE >UNREMARKABLE MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL CERVIX (CV) : -DILATATION,-SLIGHT, FOCAL -B-POLYP, ENDOMETRIAL STROMAL,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-SLIGHT, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2700 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1096 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91218 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 329.4 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:41 PROSECTOR: JODI THIEREN RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2701 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1097 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91219 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 252.9 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 12:00 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; RED HAIR COAT; HEAD- -DILATATION, VENTRICULAR,-PRESENT ENTIRE; VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : >EXCRETION; FEW FECES; VERIFIED AT -CAST, PROTEINACEOUS,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: NOT APPLICABLE -ANGIECTASIS,-SLIGHT, MULTI-FOCAL >MASS(ES); VENTRAL-ABDOMINAL; WEEK 88; -DEGENERATION, CYSTIC, CORTICAL,- 10 X 10MM; VERIFIED AT NECROPSY: SLIGHT, FOCAL OBSERVED -HEMORRHAGE,-SLIGHT, MULTI-FOCAL >APPEARANCE; HUNCHED; VERIFIED AT -PIGMENT,-MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE LUNG (LU) : >APPEARANCE; THIN; VERIFIED AT -MINERALIZATION,-MINIMAL, FOCAL NECROPSY: OBSERVED PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 7 X 7 MM, RED, DARK -HEMORRHAGE,-MODERATELY SEVERE, MULTI- RED, TAN AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-MINIMAL, MULTI-FOCAL SINGLE MASS, 18 X 13 X 8 MM, RED, TAN -B-ADENOMA,-NEOPLASM PRESENT AND FIRM; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (INGUINAL) NOT IDENTIFIED 2702 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1098 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91219 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 252.9 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 12:00 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; MUCOSA: GREEN >UNREMARKABLE MUCOID MATERIAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; DARK RED, DRY MATERIAL -DISCHARGE-NASAL; DARK RED, DRY MATERIAL -MALOCCLUSION; BILATERAL UPPER INCISORS -ANIMAL THIN -------------------------------------------- 2703 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1099 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91219 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 252.9 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 12:00 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2704 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1100 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91220 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 622.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:40 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 82; 70 X 70MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : LIVER (LI) : >MASS(ES); VENTRAL-ABDOMINAL; WEEK 82; -RED FOCUS(I)/AREA(S); ALL LOBES: -DEGENERATION, CYSTIC,-MINIMAL, FOCAL 20 X 20MM; VERIFIED AT NECROPSY: MULTIPLE RED AREAS, UP TO 2 MM IN -FOCUS, ALTERED HEPATOCELLULAR, OBSERVED DIAMETER; COLLECTED ON ROUTINE EOSINOPHILIC,-MODERATE, MULTI-FOCAL >MASS(ES); AXILLARY REGION-RIGHT; WEEK SECTIONS -FOCUS, ALTERED HEPATOCELLULAR, 84; 40 X 35MM; VERIFIED AT NECROPSY: BASOPHILIC,-MINIMAL, FOCAL OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; FOCAL WEEK 97; 40 X 30MM; VERIFIED AT -VACUOLATION, HEPATOCELLULAR,-SLIGHT, NECROPSY: OBSERVED MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL PANCREAS (PA) : PANCREAS (PA) : -MASS(ES); WITHIN PANCREAS, NEAR -HEMORRHAGE,-MINIMAL, MULTI-FOCAL SPLEEN: SINGLE MASS, 14 X 10 X 8 MM, -B-ADENOMA, ISLET CELL,-NEOPLASM RED, DARK RED, TAN, LOBULATED, FIRM, PRESENT AND VASCULAR; SAME ON CUT SURFACE; COLLECTED INTACT WITH PANCREAS; REGIONAL LN (SEE ANTERIOR MESENTERIC) THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 2705 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1101 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91220 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 622.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:40 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 6 MM, RED, DARK -PIGMENT,-MINIMAL, MULTI-FOCAL RED, TAN, LOBULATED, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL SINGLE MASS, 40 X 35 X 25 MM, RED, -PIGMENT,-MODERATE, FOCAL DARK RED, TAN, FIRM, AND LOBULATED; -B-FIBROADENOMA,- 4 NEOPLASMS PRESENT SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 35 X 30 X 20 MM, RED, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: COLLECTED ON ROUTINE SECTION -MASS #3; LEFT INGUINAL REGION: SINGLE MASS, 23 MM IN DIAMETER, RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #4; LEFT INGUINAL REGION: SINGLE MASS, 70 X 70 X 50 MM, RED, DARK RED, TAN, FIRM, LOBULATED, AND VASCULAR; SOME SECTIONS EXUDE A TAN CREAMY SEMIFLUID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : -DILATATION,-SEVERE, DIFFUSE 2706 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1102 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91220 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 622.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:40 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : -CYST(S); WITHIN WALLS OF LEFT HORN: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN; -HEMORRHAGE,-MINIMAL, MULTI-FOCAL COLLECTED -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -DIFFUSELY RED; (CAP 21) -HEMORRHAGE,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2707 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1103 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91220 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 622.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:40 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), URINARY BLADDER (UB), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2708 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1104 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91221 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 591.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:42 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 MM IN DIAMETER, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- TAN AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -GALACTOCELE,-SLIGHT, MULTI-FOCAL AND FIRM; COLLECTED ON ROUTINE SECTION 2709 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91221 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 591.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:42 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2710 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1106 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91222 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 242.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:43 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.3014 3.0159 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2711 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1107 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91222 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 242.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:43 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2712 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1108 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91223 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 213.9 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:06 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0320 .0150 % .0181 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0307 .0144 % .0173 WEIGHT TAKEN BRAIN (BR) 1.7695 .8273 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) .7838 .3664 % .4430 WEIGHT TAKEN RIGHT KIDNEY (KD1) .7669 .3585 % .4334 WEIGHT TAKEN LIVER (LI) 6.6720 3.1192 % 3.7706 WEIGHT TAKEN LUNG (LU) 1.0099 .4721 % .5707 WEIGHT TAKEN LEFT OVARY (OV0) .0434 .0203 % .0245 WEIGHT TAKEN RIGHT OVARY (OV1) .0275 .0129 % .0155 WEIGHT TAKEN SPLEEN (SP) .3311 .1548 % .1871 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0061 .0029 % .0034 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0098 .0046 % .0055 WEIGHT TAKEN UTERUS (UT) .4363 .2040 % .2466 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2713 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1109 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91223 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 213.9 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:06 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2714 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1110 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91224 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/02/99 STUDY DAY OF DEATH: 676 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 325.5 GRAMS DATE AND TIME OF NECROPSY: 12/02/99 14:29 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT OBSERVED LIVER (LI) : LIVER (LI) : >DISCHARGE; GENITAL; RED IN COLOR; -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC -TELANGIECTASIS,-SLIGHT, MULTI-FOCAL VERIFIED AT NECROPSY: OBSERVED SURFACE OF RIGHT MEDIAN LOBE: SINGLE DARK RED AREA, 7 MM IN DIAMETER; SUNKEN WITH A STELLATE PATTERN; COLLECTED ON ROUTINE SECTION KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, FOCAL -THROMBUS,-MINIMAL, FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 4 MM IN DIAMETER PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 8 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GREY, TAN AND SOFT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL 2715 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1111 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91224 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/02/99 STUDY DAY OF DEATH: 676 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 325.5 GRAMS DATE AND TIME OF NECROPSY: 12/02/99 14:29 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); INGUINAL REGIONS, DIFFUSE: -GALACTOCELE,-SLIGHT, MULTI-FOCAL MULTIPLE TAN CREAMY FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN LUMEN: -DILATATION,-MODERATE, DIFFUSE MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED -DISCHARGE-NASAL; BILATERAL: RED -------------------------------------------- 2716 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1112 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91224 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/02/99 STUDY DAY OF DEATH: 676 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 325.5 GRAMS DATE AND TIME OF NECROPSY: 12/02/99 14:29 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2717 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1113 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91225 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/02/99 STUDY DAY OF DEATH: 585 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 446.7 GRAMS DATE AND TIME OF NECROPSY: 09/02/99 8:07 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; MISSING; TEETH; VERIFIED KIDNEY (KD) : AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >APPEARANCE; THIN; VERIFIED AT FOCAL NECROPSY: NOT OBSERVED -MINERALIZATION, PELVIC,-MODERATE, >EXCRETION; FEW FECES; VERIFIED AT MULTI-FOCAL NECROPSY: NOT APPLICABLE SPLEEN (SP) : >APPEARANCE; SWOLLEN; VENTRAL- -HEMATOPOIESIS, EXTRAMEDULLARY, ABDOMINAL; VERIFIED AT NECROPSY: INCREASED,-MODERATE, DIFFUSE OBSERVED PANCREAS (PA) : PANCREAS (PA) : >SKIN & PELAGE; COLD TO TOUCH; ENTIRE -LARGE VESSELS; UP TO 3 MM IN -I-ENDOMETRIAL STROMAL SARCOMA,- BODY; VERIFIED AT NECROPSY: NOT DIAMETER; COLLECTED INTACT NEOPLASM PRESENT APPLICABLE ADRENAL, CORTEX (AC) : >SKIN & PELAGE; PALE; ENTIRE BODY; -ANGIECTASIS,-SLIGHT, MULTI-FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE -DEGENERATION, CYSTIC, CORTICAL,- >EYES; PALE; EYES; VERIFIED AT MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -LARGE; UP TO 5 MM IN DIAMETER >TISSUE MISSING OVARY (OV) : OVARY (OV) : -MISSING/NOT IDENTIFIED; LEFT: NOT -ONE EXAMINED,-PRESENT POSITIVELY IDENTIFIED; POSSIBLE REMNANTS COLLECTED 2718 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1114 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91225 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/02/99 STUDY DAY OF DEATH: 585 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 446.7 GRAMS DATE AND TIME OF NECROPSY: 09/02/99 8:07 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : THYROID (TY) : -DIFFUSELY RED; LEFT: DARK RED -CYST, ULTIMOBRANCHIAL,-PRESENT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 5 X 4 MM, DARK RED AND -HEMORRHAGE,-MODERATE, MULTI-FOCAL FIRM -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: SINGLE >UNREMARKABLE DARK RED LINEAR AREA, 3 X 1 MM CERVIX (CV) : CERVIX (CV) : -MASS(ES); INVOLVING ENTIRE CERVIX: -HEMORRHAGE,-MODERATELY SEVERE, MULTI- MULTIPLE MASSES, UP TO 64 X 34 X 33 FOCAL MM, ALL ARE RED, DARK RED, TAN, -NECROSIS,-MODERATELY SEVERE, MULTI- GELATINOUS, LOBULATED AND SOFT; SAME FOCAL ON CUT SURFACE; COLLECTED INTACT; -M-SARCOMA, ENDOMETRIAL STROMAL,- REGIONAL LN (SEE LUMBAR) POSSIBLY NEOPLASM PRESENT CONSISTENT WITH SWOLLEN ABDOMEN NOTED IN CLINICAL OBSERVATIONS CAUSE OF DEATH (CD) : -ENDOMETRIAL STROMAL SARCOMA,-PRESENT CAVITY, ABDOM (PC) : -I-ENDOMETRIAL STROMAL SARCOMA,- NEOPLASM PRESENT LN, LUMBAR/ILIAC (LM) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 2719 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1115 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91225 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/02/99 STUDY DAY OF DEATH: 585 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 446.7 GRAMS DATE AND TIME OF NECROPSY: 09/02/99 8:07 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL: RED AND TAN (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -TEETH MISSING/BROKEN/WORN; BROKEN AT BASE: BILATERAL, UPPER INCISORS -------------------------------------------- 2720 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1116 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91225 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/02/99 STUDY DAY OF DEATH: 585 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 446.7 GRAMS DATE AND TIME OF NECROPSY: 09/02/99 8:07 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2721 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1117 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91226 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 377.8 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:15 PROSECTOR: TERRI L. WILLIAMS RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 59; 40 X 45MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); INGUINAL REGION-LEFT; WEEK KIDNEY (KD) : 64; 40 X 60MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MODERATE, OBSERVED FOCAL >MASS(ES); INGUINAL REGION-RIGHT; WEEK -NEPHROPATHY, CHRONIC PROGRESSIVE,- 74; 40 X 40MM; VERIFIED AT NECROPSY: MINIMAL, MULTI-FOCAL OBSERVED ADRENAL, CORTEX (AC) : >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK -DEGENERATION, CYSTIC, CORTICAL,- 78; 30 X 30MM; VERIFIED AT NECROPSY: MODERATE, FOCAL OBSERVED -HEMORRHAGE,-MODERATE, FOCAL >APPEARANCE; THIN; VERIFIED AT OVARY (OV) : OVARY (OV) : NECROPSY: NOT OBSERVED -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT >APPEARANCE; HEAD TILT; LEFT; VERIFIED FILLED CYST, 3 MM IN DIAMETER -MINERALIZATION,-MINIMAL, FOCAL AT NECROPSY: NOT APPLICABLE LUNG (LU) : >RESPIRATION; AUDIBLE; VERIFIED AT -MINERALIZATION, VASCULAR,-MINIMAL, NECROPSY: NOT APPLICABLE FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT THYROID (TY) : NECROPSY: NOT APPLICABLE -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 8 X 7 MM, RED, DARK -HEMORRHAGE,-MINIMAL, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT 2722 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1118 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91226 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 377.8 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:15 PROSECTOR: TERRI L. WILLIAMS RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL SINGLE MASS, 30 X 25 X 15 MM, RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL DARK RED, FIRM AND LOBULATED; ON CUT -B-FIBROADENOMA,- 3 NEOPLASMS PRESENT SURFACE EXUDES A TAN SEMISOLID; -M-CARCINOMA,-NEOPLASM PRESENT COLLECTED; REGIONAL LN (SEE AXILLARY) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY FLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 45 X 40 X 20 MM, RED, DARK RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #3; LEFT INGUINAL REGION: SINGLE MASS, 60 X 38 X 30 MM, RED, DARK RED, TAN, BROWN, LOBULATED AND FIRM; SOME SECTIONS EXUDE A TAN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED 2723 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1119 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91226 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 377.8 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:15 PROSECTOR: TERRI L. WILLIAMS RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #4; RIGHT INGUINAL REGION: SINGLE MASS, 36 X 24 X 24 MM, RED, DARK RED, TAN AND FIRM; ON SECTION, FILLED WITH A DARK BROWN FLUID AND A TAN SEMISOLID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP >UNREMARKABLE 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED -------------------------------------------- 2724 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1120 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91226 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 377.8 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:15 PROSECTOR: TERRI L. WILLIAMS RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2725 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1121 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91227 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 527.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:34 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-RIGHT; WEEK LIVER (LI) : 84; 100 X 70MM; VERIFIED AT NECROPSY: -VACUOLATION, HEPATOCELLULAR,-MINIMAL, OBSERVED MULTI-FOCAL >MASS(ES); INGUINAL REGION-LEFT; WEEK KIDNEY (KD) : 86; 85 X 65MM; VERIFIED AT NECROPSY: -CAST, PROTEINACEOUS,-MINIMAL, MULTI- OBSERVED FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -MASS(ES); INVOLVING ENTIRE RIGHT -DEGENERATION, CYSTIC, CORTICAL,- GLAND: SINGLE MASS, 10 MM IN DIAMETER, SEVERE, FOCAL DARK RED, BROWN AND FIRM; COLLECTED; -HEMORRHAGE,-MODERATELY SEVERE, MULTI- REGIONAL LN (RENAL) NOT IDENTIFIED FOCAL -THROMBUS,-MODERATE, FOCAL LUNG (LU) : -HYPERPLASIA, TYPE II PNEUMOCYTE,- SLIGHT, MULTI-FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -RAISED AREA(S); LEFT ANTERIOR ASPECT: -B-ADENOMA,-NEOPLASM PRESENT SINGLE DARK RED FOCUS, 1 MM IN DIAMETER MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT 2726 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1122 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91227 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 527.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:34 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); LEFT PERINEAL AND PERIANAL REGIONS: SINGLE MASS, 80 X 55 X 25 MM (WEIGHS 58.2 GRAMS), TAN, GREY, RED, FIRM AND VASCULAR; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT INGUINAL REGION, EXTENDING INTO RIGHT PERIANAL REGION: SINGLE MASS, 100 X 60 X 40 MM (WEIGHS 125.2 GRAMS), TAN, RED, FIRM AND VASCULAR; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2727 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1123 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91227 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 527.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:34 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2728 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1124 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91228 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 313.5 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:40 PROSECTOR: JODI THIEREN RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 82; 25 X 20MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; RED HAIR COAT; HEAD- KIDNEY (KD) : ENTIRE; VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-SLIGHT, MULTI- >EYES; RED DISCHARGE; EYES; VERIFIED AT FOCAL NECROPSY: OBSERVED ADRENAL, CORTEX (AC) : >SKIN & PELAGE; BROWN HAIR COAT; -ANGIECTASIS,-MODERATE, MULTI-FOCAL VENTRAL-ABDOMINAL; VERIFIED AT -HEMORRHAGE,-MODERATE, MULTI-FOCAL NECROPSY: OBSERVED ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT OVARY (OV) : -CYST,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 9 X 7 MM, RED, DARK -CYST,-PRESENT RED, LOBULATED AND FIRM -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EDEMA,-MODERATELY SEVERE, DIFFUSE MULTIPLE DARK BROWN PINPOINT FOCI -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL 2729 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1125 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91228 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 313.5 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:40 PROSECTOR: JODI THIEREN RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL SINGLE MASS, 30 X 27 X 15 MM, TAN, -B-FIBROADENOMA,-NEOPLASM PRESENT GREY, LOBULATED AND FIRM: SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE LIGHT GREEN MUCOID SEMIFLUID MATERIAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP >UNREMARKABLE 21) GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: MOIST, BROWN MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, DARK RED MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: DRY, RED MATERIAL -------------------------------------------- 2730 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1126 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91228 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 313.5 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:40 PROSECTOR: JODI THIEREN RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2731 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1127 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91229 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 251.4 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:40 PROSECTOR: JARED FISHEL RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 82; 50 X 45MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; BLUE SKIN; VENTRAL- LIVER (LI) : LIVER (LI) : ABDOMINAL-LEFT; VERIFIED AT NECROPSY: -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC -FIBROSIS,-MODERATE, MULTI-FOCAL NOT APPLICABLE SURFACE OF LEFT LATERAL LOBE: SINGLE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >APPEARANCE; THIN; VERIFIED AT TAN AREA, 15 X 10 MM; COLLECTED ON FOCAL NECROPSY: OBSERVED ROUTINE SECTION -NECROSIS, HEPATOCELLULAR,-MODERATE, >RESPIRATION; IRREGULAR; VERIFIED AT MULTI-FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 7 MM IN -ANGIECTASIS,-MODERATELY SEVERE, MULTI- DIAMETER FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL OVARY (OV) : -MINERALIZATION,-MINIMAL, MULTI-FOCAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT 2732 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1128 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91229 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 251.4 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:40 PROSECTOR: JARED FISHEL RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT POSITIVELY IDENTIFIED; POSSIBLE REMNANTS COLLECTED THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 8 X 8 MM, DARK RED AND -CYST,-PRESENT SOFT -HEMORRHAGE,-MODERATELY SEVERE, MULTI- FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : >NOTE:>ROUTINE SECTION TAKEN FROM RIGHT INGUINAL REGION DUE TO MASS ON LEFT INGUINAL REGION MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL SINGLE MASS, 45 X 40 X 35 MM, DARK -MINERALIZATION,-MINIMAL, MULTI-FOCAL RED, BROWN, TAN, LOBULATED AND SOFT; -M-CARCINOMA,-NEOPLASM PRESENT ON SECTION EXUDES A BROWN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 21) >NOTE:>ROUTINE SECTION TAKEN FROM RIGHT INGUINAL REGION DUE TO MASS ON LEFT INGUINAL REGION VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE LIGHT GREEN MUCOID SEMIFLUID MATERIAL 2733 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1129 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91229 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 251.4 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:40 PROSECTOR: JARED FISHEL RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN), LN, INGUINAL (IN) ------------------------------------------------------------------------------------------------------------------------------------ 2734 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1130 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91230 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 209.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:30 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 6.6675 3.1765 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2735 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1131 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91230 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 209.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:30 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2736 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1132 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91231 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/26/99 STUDY DAY OF DEATH: 639 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 467.1 GRAMS DATE AND TIME OF NECROPSY: 10/26/99 14:06 PROSECTOR: JOHN S. HALFORD RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; HEAD- BRAIN (BR) : BRAIN (BR) : ENTIRE; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: OBSERVED LIVER (LI) : >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -VACUOLATION, HEPATOCELLULAR,-MODERATE, AT NECROPSY: NOT OBSERVED MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >RESPIRATION; LABORED; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MODERATE, >DISCHARGE; NASAL; RED IN COLOR; MULTI-FOCAL VERIFIED AT NECROPSY: OBSERVED ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL -VACUOLATION,-MODERATE, FOCAL OVARY (OV) : -CYST,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 8 X 8 MM, RED, GRAY, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT 2737 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1133 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91231 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/26/99 STUDY DAY OF DEATH: 639 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 467.1 GRAMS DATE AND TIME OF NECROPSY: 10/26/99 14:06 PROSECTOR: JOHN S. HALFORD RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA, TISSUE >UNREMARKABLE SURROUNDING PYLORIC VALVE: MULTIPLE DARK BROWN FOCI, UP TO 1 MM IN DIAMETER -THICKENED WALL; MUCOSA STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -EDEMA,-SLIGHT, MULTI-FOCAL TAN, SLIGHTLY ERODED AREAS, UP TO 2 X -INFLAMMATION, ACUTE,-SLIGHT, MULTI- 1 MM FOCAL -THICKENED WALL; MUCOSA SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); ATTACHED TO RIGHT -ACUTE INFLAMMATORY EXUDATE,-MODERATELY LOWER LIP: SINGLE DRY AND TAN CRUSTED SEVERE, FOCAL AREA, 5 X 3 MM (CAP 21) MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO OMENTUM, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL CRANIAL TO LEFT OVARY: SINGLE MASS, 25 -NECROSIS, FAT,-PRESENT X 10 X 10 MM, DARK BROWN, GRAY, TAN, GREEN AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (MESENTERIC) NORMAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: BROWN, MOIST MATERIAL 2738 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1134 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91231 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/26/99 STUDY DAY OF DEATH: 639 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 467.1 GRAMS DATE AND TIME OF NECROPSY: 10/26/99 14:06 PROSECTOR: JOHN S. HALFORD RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -DISCHARGE-NASAL; DRY, RED MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: DRY, RED MATERIAL -ANIMAL OBESE -------------------------------------------- 2739 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1135 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91231 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/26/99 STUDY DAY OF DEATH: 639 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 467.1 GRAMS DATE AND TIME OF NECROPSY: 10/26/99 14:06 PROSECTOR: JOHN S. HALFORD RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2740 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1136 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91232 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 398.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:45 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-SLIGHT, FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT CERVIX (CV) : CERVIX (CV) : -MASS(ES); WITHIN LUMEN: SINGLE MASS, -B-POLYP, ENDOMETRIAL STROMAL,-NEOPLASM 9 X 8 X 6 MM, TAN AND FIRM; SAME ON PRESENT CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 20) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2741 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1137 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91232 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 398.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:45 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 2742 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1138 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91233 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 247.1 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:25 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0433 .0175 % .0217 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0344 .0139 % .0173 WEIGHT TAKEN BRAIN (BR) 1.9921 .8062 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.0609 .4293 % .5326 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.0182 .4121 % .5111 WEIGHT TAKEN LIVER (LI) 7.5938 3.0732 % 3.8120 WEIGHT TAKEN LUNG (LU) 1.1176 .4523 % .5610 WEIGHT TAKEN LEFT OVARY (OV0) .0518 .0210 % .0260 WEIGHT TAKEN RIGHT OVARY (OV1) .0586 .0237 % .0294 WEIGHT TAKEN SPLEEN (SP) .4480 .1813 % .2249 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0064 .0026 % .0032 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0129 .0052 % .0065 WEIGHT TAKEN UTERUS (UT) .4988 .2019 % .2504 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2743 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1139 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91233 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 247.1 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:25 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2744 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1140 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91234 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/29/99 STUDY DAY OF DEATH: 459 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/29/99 11:13 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : LIVER (LI) : -MOTTLED; ALL LOBES: BROWN AND TAN -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL -MINERALIZATION, TUBULAR,-MINIMAL, FOCAL -PYELONEPHRITIS,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 7 X 6 MM, DARK RED, -HEMORRHAGE,-SEVERE, MULTI-FOCAL RED, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -EDEMA,-MODERATE, FOCAL TAN ERODED AREAS, UP TO 3 X 2 MM -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT -------------------------------------------- 2745 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1141 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91234 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/29/99 STUDY DAY OF DEATH: 459 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/29/99 11:13 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2746 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1142 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91235 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 454.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:34 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); JUNCTION OF -LIPIDOSIS, SUBCAPSULAR (TENSION RIGHT AND LEFT MEDIAN LOBES: SINGLE LIPIDOSIS),-SEVERE, FOCAL TAN AREA, 4 X 3 MM; EXTRA SECTION COLLECTED KIDNEY (KD) : KIDNEY (KD) : -GRANULAR MATERIAL; BILATERAL, WITHIN -MINERALIZATION, PELVIC,-MODERATE, PELVIS: MULTIPLE WHITE PINPOINT FOCAL GRANULES PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -CYST(S); RIGHT, WITHIN CORTEX: -ANGIECTASIS,-MODERATELY SEVERE, MULTI- MULTIPLE RED FLUID FILLED CYSTS, UP TO FOCAL 2 MM IN DIAMETER -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -THROMBUS,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); LEFT ANTERIOR -HEMORRHAGE,-MODERATE, MULTI-FOCAL ASPECT: SINGLE DARK BROWN AREA, 2 MM -B-ADENOMA,-NEOPLASM PRESENT IN DIAMETER MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT 2747 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1143 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91235 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 454.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:34 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CERVIX (CV) : CERVIX (CV) : -LARGE; DIFFUSE: 8 MM IN DIAMETER -HYPERTROPHY, SMOOTH MUSCLE,-MINIMAL, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -LARGE; UP TO 4 X 3 X 1 MM >TISSUE MISSING -DIFFUSELY RED; DARK RED (CAP 20) >NOTE:>MEDIASTINAL LYMPH NODE DESCRIBED GROSSLY WAS ADIPOSE TISSUE ADIPOSE TISSUE (AT) : -FIBROSIS,-MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2748 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1144 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91235 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 454.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:34 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2749 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1145 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91236 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 442.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:43 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 93; 15 X 15MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : LIVER (LI) : -DARK FOCUS(I)/AREA(S); ALL LOBES: -TELANGIECTASIS,-SLIGHT, FOCAL MULTIPLE DARK BROWN AREAS, UP TO 4 X 3 -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MM; COLLECTED ON ROUTINE SECTIONS MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MINIMAL, FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 5 MM IN DIAMETER LUNG (LU) : -HYPERPLASIA, TYPE II PNEUMOCYTE,- MODERATE, MULTI-FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- MODERATE, MULTI-FOCAL THYROID (TY) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 5 X 5 X 4 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, DARK RED, DARK BROWN AND SOFT -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2750 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1146 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91236 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 442.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:43 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 30 X 20 X 15 MM, TAN AND -B-FIBROADENOMA,-NEOPLASM PRESENT FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2751 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1147 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91236 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 442.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:43 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), URINARY BLADDER (UB), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2752 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1148 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91237 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 194.6 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:00 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 6.4697 3.3246 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2753 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1149 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91237 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 194.6 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:00 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2754 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1150 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91238 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/10/99 STUDY DAY OF DEATH: 470 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 280.5 GRAMS DATE AND TIME OF NECROPSY: 05/10/99 13:53 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT OBSERVED LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, RED, BROWN, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- TAN, AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 20 X 15 X 10 MM, TAN, -B-ADENOMA,-NEOPLASM PRESENT FIRM, AND LOBULATED; SAME ON CUT -B-FIBROADENOMA,-NEOPLASM PRESENT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2755 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1151 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91238 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/10/99 STUDY DAY OF DEATH: 470 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 280.5 GRAMS DATE AND TIME OF NECROPSY: 05/10/99 13:53 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; CHEST AND FORELIMBS: RED AND DRY -GI TRACT-DARK FLUID CONTENT; ENTIRE GI TRACT: DARK BROWN, TENACIOUS SEMIFLUID -------------------------------------------- 2756 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1152 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91238 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/10/99 STUDY DAY OF DEATH: 470 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 280.5 GRAMS DATE AND TIME OF NECROPSY: 05/10/99 13:53 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2757 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1153 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91239 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/01/99 STUDY DAY OF DEATH: 400 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 399.2 GRAMS DATE AND TIME OF NECROPSY: 03/01/99 13:43 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); AXILLARY REGION-RIGHT; WEEK LIVER (LI) : 35; 45 X 45MM; VERIFIED AT NECROPSY: -HEMATOPOIESIS, EXTRAMEDULLARY,- OBSERVED MINIMAL, MULTI-FOCAL >MASS(ES); AXILLARY REGION-LEFT; WEEK KIDNEY (KD) : 47; 10 X 10MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MODERATE, OBSERVED FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 7 X 4 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, DARK RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT 2758 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1154 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91239 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/01/99 STUDY DAY OF DEATH: 400 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 399.2 GRAMS DATE AND TIME OF NECROPSY: 03/01/99 13:43 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 60 X 55 X 25 MM, RED, DARK RED, TAN, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED; OVERLYING SKIN HAS A SINGLE RED AND DRY ULCERATED AREA, 20 X 17 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (AXILLARY) NORMAL (CAP 21) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN AND CREAMY SEMIFLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION AND EXTRA SECTION COLLECTED (CAP 20); POSSIBLY CONSISTENT WITH MASS (LEFT AXILLARY) NOTED IN CLINICAL OBSERVATIONS UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -DILATATION,-MODERATELY SEVERE, DIFFUSE MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 9 X 6 X 5 MM CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -------------------------------------------- 2759 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1155 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91239 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/01/99 STUDY DAY OF DEATH: 400 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 399.2 GRAMS DATE AND TIME OF NECROPSY: 03/01/99 13:43 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2760 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1156 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91240 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/29/99 STUDY DAY OF DEATH: 550 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 382.6 GRAMS DATE AND TIME OF NECROPSY: 07/29/99 15:27 PROSECTOR: JILL PAUS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); AXILLARY REGION-RIGHT; WEEK -DILATATION, VENTRICULAR,-PRESENT 74; 10 X 10MM; VERIFIED AT NECROPSY: KIDNEY (KD) : OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >RESPIRATION; LABORED; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 5 MM, RED, DARK -HEMORRHAGE,-SLIGHT, MULTI-FOCAL RED, TAN, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL SINGLE MASS, 25 X 18 X 15 MM, RED, -MINERALIZATION,-MINIMAL, MULTI-FOCAL DARK RED, TAN, AND FIRM; SAME ON CUT -B-ADENOMA,-NEOPLASM PRESENT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) UTERUS (UT) : -DILATATION,-SEVERE, FOCAL 2761 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1157 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91240 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/29/99 STUDY DAY OF DEATH: 550 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 382.6 GRAMS DATE AND TIME OF NECROPSY: 07/29/99 15:27 PROSECTOR: JILL PAUS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : -CYST(S); LEFT, WITHIN HORN: SINGLE CLEAR FLUID FILLED CYST, 22 X 10 X 10 MM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: UPPER INCISORS -------------------------------------------- 2762 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1158 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91240 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/29/99 STUDY DAY OF DEATH: 550 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 382.6 GRAMS DATE AND TIME OF NECROPSY: 07/29/99 15:27 PROSECTOR: JILL PAUS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2763 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1159 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91241 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 287.7 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 9:12 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0404 .0140 % .0202 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0423 .0147 % .0211 WEIGHT TAKEN BRAIN (BR) 2.0015 .6957 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.0454 .3634 % .5223 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.1192 .3890 % .5592 WEIGHT TAKEN LIVER (LI) 9.1320 3.1741 % 4.5626 WEIGHT TAKEN LUNG (LU) 1.3728 .4772 % .6859 WEIGHT TAKEN LEFT OVARY (OV0) .0433 .0151 % .0216 WEIGHT TAKEN RIGHT OVARY (OV1) .0524 .0182 % .0262 WEIGHT TAKEN SPLEEN (SP) .5501 .1912 % .2748 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0081 .0028 % .0040 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0083 .0029 % .0041 WEIGHT TAKEN UTERUS (UT) .6220 .2162 % .3108 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL NECROPSIED OUT OF ORDER DUE TO MORIBUND COLLECTION FOLLOWING SAMPLE COLLECTION 2764 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1160 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91241 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 287.7 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 9:12 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2765 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1161 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91242 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 437.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:13 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR -CYST,-PRESENT FLUID FILLED CYST ON EACH, LEFT IS 6 MM IN DIAMETER, RIGHT IS 9 MM IN DIAMETER LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN >UNREMARKABLE AND FIRM; COLLECTED ON ROUTINE SECTION UTERUS (UT) : UTERUS (UT) : -MASS(ES); MID PORTION OF RIGHT HORN, -DILATATION,-MODERATE, FOCAL WITHIN LUMEN: SINGLE MASS, 7 X 5 X 5 -B-POLYP, ENDOMETRIAL, STROMAL,- MM, RED, DARK RED AND SOFT; SAME ON NEOPLASM PRESENT CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 20) 2766 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1162 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91242 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 437.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:13 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 2767 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1163 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91243 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 564.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:33 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 68; 75 X 60MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE OBSERVED LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; SORE/SCAB; DORSAL; -LIGHT FOCUS(I)/AREA(S); CAUDATE LOBE: -FOCUS, ALTERED HEPATOCELLULAR, VERIFIED AT NECROPSY: OBSERVED SINGLE TAN AREA, 6 MM IN DIAMETER; EOSINOPHILIC,-SLIGHT, FOCAL >MASS(ES); INGUINAL REGION-LEFT; WEEK COLLECTED -FOCUS, ALTERED HEPATOCELLULAR, 89; 30 X 40MM; VERIFIED AT NECROPSY: -RED FOCUS(I)/AREA(S); ALL LOBES: BASOPHILIC,-SLIGHT, FOCAL OBSERVED MULTIPLE DARK RED AREAS, UP TO 3 MM IN >MASS(ES); INGUINAL REGION-RIGHT; WEEK DIAMETER; COLLECTED ON ROUTINE 93; 25 X 25MM; VERIFIED AT NECROPSY: SECTIONS OBSERVED KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-SLIGHT, FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: MULTIPLE CLEAR -CYST,-PRESENT FLUID FILLED CYSTS ON EACH, UP TO 2 MM -HEMORRHAGE,-MINIMAL, FOCAL IN DIAMETER LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, FOCAL THYROID (TY) : -HEMORRHAGE,-SLIGHT, FOCAL 2768 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1164 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91243 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 564.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:33 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : THYROID (TY) : -LARGE; BILATERAL: EACH IS 7 X 4 X 4 -B-ADENOMA, C-CELL,-NEOPLASM PRESENT MM PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 5 X 5 MM, TAN, SOFT AND -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LOBULATED -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : SKIN (SK) : -MASS(ES); DORSAL THORACIC REGION: -INFLAMMATION, ACUTE,-SLIGHT, FOCAL SINGLE MASS, 15 MM IN DIAMETER, TAN -ULCERATION,-SLIGHT, FOCAL AND FIRM; SAME ON CUT SURFACE; -M-SEBACEOUS GLAND CARCINOMA,-NEOPLASM COLLECTED; OVERLYING SURFACE HAS A PRESENT SINGLE DARK RED AND DRY CRUSTED AREA, 5 MM IN DIAMETER; COLLECTED INTACT WITH MASS; REGIONAL LN (SEE AXILLARY) MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT PERIANAL REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 60 X 40 X 25 MM, RED, -MINERALIZATION,-MINIMAL, MULTI-FOCAL DARK RED, TAN AND LOBULATED; ON -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT SECTION EXUDES A DARK GREEN SEMISOLID; -M-CARCINOMA,-NEOPLASM PRESENT COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 45 X 35 X 25 MM, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED 2769 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1165 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91243 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 564.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:33 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #3; LEFT AXILLARY REGION: SINGLE MASS, 90 X 50 X 30 MM, TAN, FIRM AND LOBULATED; SOME SECTIONS EXUDE A LARGE AMOUNT OF A TAN, CREAMY SEMIFLUID; PORTION COLLECTED; REGIONAL LN (SEE AXILLARY) UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MULTIPLE CLEAR FLUID FILLED CYSTS, UP MODERATE, MULTI-FOCAL TO 5 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS UP TO 10 X -DILATATION, SINUSOIDAL,-MINIMAL, MULTI- 6 X 3 MM (CAP 20) FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2770 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1166 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91243 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 564.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:33 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2771 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1167 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91244 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/07/99 STUDY DAY OF DEATH: 467 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 316.8 GRAMS DATE AND TIME OF NECROPSY: 05/07/99 13:57 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >BEHAVIOR; VOCALIZATION; VERIFIED AT -CAST, PROTEINACEOUS,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >RESPIRATION; AUDIBLE; VERIFIED AT -MINERALIZATION, PELVIC,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT, MULTI- FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYROID (TY) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 7 X 6 MM, -B-ADENOMA,-NEOPLASM PRESENT TAN, RED AND SOFT VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE CONTAINS A LIGHT GREEN SEMIFLUID 2772 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1168 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91244 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/07/99 STUDY DAY OF DEATH: 467 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 316.8 GRAMS DATE AND TIME OF NECROPSY: 05/07/99 13:57 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DARK RED AND DRY -MALOCCLUSION; BILATERAL UPPER INCISORS -------------------------------------------- 2773 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1169 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91244 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/07/99 STUDY DAY OF DEATH: 467 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 316.8 GRAMS DATE AND TIME OF NECROPSY: 05/07/99 13:57 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2774 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1170 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91245 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 668 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 413.7 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 14:19 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; YELLOW HAIR COAT; -DILATATION, VENTRICULAR,-PRESENT VERIFIED AT NECROPSY: NOT OBSERVED LIVER (LI) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -VACUOLATION, HEPATOCELLULAR,-MINIMAL, NECROPSY: OBSERVED MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -CAST, PROTEINACEOUS,-MINIMAL, FOCAL >SKIN & PELAGE; RED HAIR COAT; HEAD- ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : ENTIRE; VERIFIED AT NECROPSY: OBSERVED -LARGE; RIGHT: 8 X 7 X 5 MM (LEFT IS 6 -ANGIECTASIS,-SEVERE, MULTI-FOCAL X 5 X 3 MM) -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, DARK RED AND -HEMORRHAGE,-MODERATE, MULTI-FOCAL SOFT -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL 2775 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1171 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91245 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 668 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 413.7 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 14:19 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: RED -------------------------------------------- 2776 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1172 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91245 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 668 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 413.7 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 14:19 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2777 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1173 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91246 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 319.5 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 10:56 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HUNCHED; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: NOT APPLICABLE -MOTTLED; BILATERAL: TAN AND BROWN -MINERALIZATION, PELVIC,-MINIMAL, FOCAL >MASS(ES); AXILLARY REGION-LEFT; WEEK ADRENAL, CORTEX (AC) : 68; 105 X 75MM; VERIFIED AT NECROPSY: -ANGIECTASIS,-MODERATE, MULTI-FOCAL OBSERVED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL >EXCRETION; NO FECES; VERIFIED AT THYMUS (TH) : NECROPSY: NOT APPLICABLE -CYST,-PRESENT >APPEARANCE; THIN; VERIFIED AT LUNG (LU) : NECROPSY: OBSERVED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL >EYES; RED DISCHARGE; EYES; VERIFIED AT THYROID (TY) : NECROPSY: OBSERVED -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -NECROSIS,-SLIGHT, FOCAL -PIGMENT,-MINIMAL, FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE RED AREAS, UP TO 2 X 3 MM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -GALACTOCELE,-MINIMAL, MULTI-FOCAL SINGLE MASS, 25 X 55 X 100 MM (88.4 -NECROSIS,-MODERATE, MULTI-FOCAL GRAMS) FIRM, LOBULATED, RED, DARK RED, -M-CARCINOMA,-NEOPLASM PRESENT TAN AND VASCULAR; ON SECTION CONTAINS A LARGE AMOUNT OF DARK BROWN FLUID (COLLECTED); REGIONAL LN (SEE AXILLARY) 2778 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1174 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91246 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 319.5 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 10:56 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MODERATE, FOCAL CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; RIGHT: RED AND TAN (CAP 21) -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -DIFFUSELY RED; LEFT: DARK RED (CAP 21) GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 2779 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1175 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91246 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 319.5 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 10:56 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2780 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1176 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91247 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 325.5 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 14:15 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 64; 30 X 30MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -NECROSIS, HEPATOCELLULAR,-SLIGHT, >APPEARANCE; THIN; VERIFIED AT MULTI-FOCAL NECROPSY: NOT OBSERVED -PIGMENT,-SLIGHT, MULTI-FOCAL >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; KIDNEY (KD) : WEEK 70; 20 X 20MM; VERIFIED AT -CAST, PROTEINACEOUS,-MINIMAL, MULTI- NECROPSY: OBSERVED FOCAL >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED -MINERALIZATION, PELVIC,-MINIMAL, MULTI- AT NECROPSY: NOT APPLICABLE FOCAL >RESPIRATION; AUDIBLE; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: NOT APPLICABLE -ANGIECTASIS,-MODERATELY SEVERE, MULTI- >RESPIRATION; LABORED; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE -DEGENERATION, CYSTIC, CORTICAL,- >EXCRETION; NO FECES; VERIFIED AT MODERATE, FOCAL NECROPSY: NOT APPLICABLE -HEMORRHAGE,-MODERATE, MULTI-FOCAL >DISCHARGE; ORAL; RED IN COLOR; THYMUS (TH) : VERIFIED AT NECROPSY: OBSERVED -CYST,-PRESENT LUNG (LU) : LUNG (LU) : -UNCOLLAPSED; ALL LOBES -EDEMA, ALVEOLAR,-MINIMAL, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL 2781 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1177 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91247 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 325.5 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 14:15 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 14 X 9 X 8 MM, -HEMORRHAGE,-SEVERE, MULTI-FOCAL DARK RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-MODERATELY SEVERE, MULTI- SINGLE MASS, 20 X 17 X 8 MM, TAN, RED FOCAL AND SOFT; SAME ON CUT SURFACE; -MINERALIZATION,-SLIGHT, MULTI-FOCAL COLLECTED; REGIONAL LN (INGUINAL) NOT -B-FIBROADENOMA,-NEOPLASM PRESENT IDENTIFIED -M-CARCINOMA,-NEOPLASM PRESENT -CYST(S); LEFT PERIANAL REGION: TWO TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 8 X 6 X 3 MM (CAP 20) -MASS #2; RIGHT LATERAL THORACIC REGION: SINGLE MASS, 33 X 27 X 13 MM, TAN, GRAY, RED, SOFT AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NOT IDENTIFIED VAGINA (VA) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-ORAL; RED AND DRY -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED AND DRY -------------------------------------------- 2782 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1178 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91247 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 325.5 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 14:15 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2783 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1179 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91248 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 283.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:33 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0313 .0110 % .0155 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0306 .0108 % .0151 WEIGHT TAKEN BRAIN (BR) 2.0203 .7126 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) .9436 .3328 % .4671 WEIGHT TAKEN RIGHT KIDNEY (KD1) .9676 .3413 % .4789 WEIGHT TAKEN LIVER (LI) 7.5181 2.6519 % 3.7213 WEIGHT TAKEN LUNG (LU) 1.3491 .4759 % .6678 WEIGHT TAKEN LEFT OVARY (OV0) .0662 .0234 % .0328 WEIGHT TAKEN RIGHT OVARY (OV1) .0617 .0218 % .0305 WEIGHT TAKEN SPLEEN (SP) .5114 .1804 % .2531 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0118 .0042 % .0058 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0113 .0040 % .0056 WEIGHT TAKEN UTERUS (UT) .5209 .1837 % .2578 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2784 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1180 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91248 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 283.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:33 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2785 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1181 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91249 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 405.4 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:48 PROSECTOR: DAVID SCHUETTE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; PALE; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; YELLOW HAIR COAT; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: NOT OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -VACUOLATION, HEPATOCELLULAR,-MODERATE, >EXCRETION; FEW FECES; VERIFIED AT MULTIPLE DARK RED AREAS, UP TO 2 MM IN MULTI-FOCAL NECROPSY: NOT APPLICABLE DIAMETER; COLLECTED ON ROUTINE >APPEARANCE; THIN; VERIFIED AT SECTIONS NECROPSY: NOT OBSERVED KIDNEY (KD) : >DISCHARGE; NASAL; RED IN COLOR; -MINERALIZATION, PELVIC,-SLIGHT, MULTI- VERIFIED AT NECROPSY: OBSERVED FOCAL >SKIN & PELAGE; RED HAIR COAT; VENTRAL- ADRENAL, CORTEX (AC) : ABDOMINAL; VERIFIED AT NECROPSY: NOT -ANGIECTASIS,-MINIMAL, MULTI-FOCAL OBSERVED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL >RESPIRATION; LABORED; VERIFIED AT PITUITARY (PI) : PITUITARY (PI) : NECROPSY: NOT APPLICABLE -MASS(ES); 9 X 7 X 7 MM, DARK RED, TAN -HEMORRHAGE,-MODERATE, MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT AND SOFT -B-ADENOMA,-NEOPLASM PRESENT NECROPSY: NOT APPLICABLE JEJUNUM (JE) : JEJUNUM (JE) : -DIVERTICULUM(A); MID JEJUNUM: SINGLE >UNREMARKABLE DARK RED DIVERTICULUM, 12 X 12 X 10 MM; COLLECTED ON ROUTINE SECTION MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); LEFT ABDOMINAL REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL SINGLE TAN CREAMY SEMIFLUID FILLED -INFLAMMATION, ACUTE,-MODERATE, MULTI- CYST, 10 X 6 X 5 MM; COLLECTED ON FOCAL ROUTINE SECTION -PIGMENT,-SLIGHT, MULTI-FOCAL 2786 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1182 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91249 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 405.4 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:48 PROSECTOR: DAVID SCHUETTE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -MASS(ES); ANTERIOR TO RIGHT OVARY: -MINERALIZATION,-MINIMAL, MULTI-FOCAL SINGLE MASS, 22 X 20 X 12 MM, TAN AND -NECROSIS, FAT,-PRESENT FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; DRY DARK BROWN MATERIAL -------------------------------------------- 2787 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1183 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91249 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 405.4 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:48 PROSECTOR: DAVID SCHUETTE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 2788 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1184 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91250 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/14/99 STUDY DAY OF DEATH: 505 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 487.9 GRAMS DATE AND TIME OF NECROPSY: 06/14/99 15:14 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, MULTI- >MASS(ES); AXILLARY REGION-RIGHT; WEEK FOCAL 38; 100 X 100MM; VERIFIED AT NECROPSY: ADRENAL, CORTEX (AC) : OBSERVED -ANGIECTASIS,-MODERATE, MULTI-FOCAL >SKIN & PELAGE; SORE/SCAB; AXILLARY -DEGENERATION, CYSTIC, CORTICAL,- REGION-RIGHT; VERIFIED AT NECROPSY: MODERATE, FOCAL OBSERVED -HEMORRHAGE,-MODERATE, MULTI-FOCAL OVARY (OV) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SKIN (SK) : -INFLAMMATION, ACUTE,-MODERATE, FOCAL -ULCERATION,-SEVERE, FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 120 X 100 X 40 MM, RED, DARK RED, TAN, VASCULAR, AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20); OVERLYING SKIN OF MASS HAS A SINGLE DARK BROWN CRUSTED AREA, 25 X 10 MM; COLLECTED INTACT WITH MASS PORTION -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION 2789 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1185 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91250 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/14/99 STUDY DAY OF DEATH: 505 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 487.9 GRAMS DATE AND TIME OF NECROPSY: 06/14/99 15:14 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : -DILATATION,-SLIGHT, DIFFUSE CAUSE OF DEATH (CD) : -MAMMARY FIBROADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -------------------------------------------- 2790 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1186 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91250 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/14/99 STUDY DAY OF DEATH: 505 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 487.9 GRAMS DATE AND TIME OF NECROPSY: 06/14/99 15:14 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2791 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1187 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91251 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 209.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:53 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.0583 3.3756 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2792 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1188 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91251 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 209.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:53 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2793 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1189 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91252 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 600.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:06 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : VERIFIED AT NECROPSY: OBSERVED -LARGE; LEFT: 7 MM IN DIAMETER (RIGHT -DEGENERATION, CYSTIC, CORTICAL,- IS 4 MM IN DIAMETER) MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MODERATELY SEVERE, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ANTERIOR -PIGMENT,-MINIMAL, MULTI-FOCAL ASPECT: SINGLE DARK RED AREA, 5 X 3 MM -B-ADENOMA,-NEOPLASM PRESENT -LARGE; 7 X 7 X 3 MM; APPEARS SWOLLEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2794 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1190 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91252 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 600.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:06 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LUNG (LU), MAMMARY, FEMALE (MF), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2795 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1191 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91253 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 542.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:35 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK LIVER (LI) : 105; 30 X 30MM; VERIFIED AT NECROPSY: -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- OBSERVED FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 5 X 4 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL SINGLE MASS, 50 X 34 X 20 MM, TAN, -B-FIBROADENOMA,-NEOPLASM PRESENT LOBULATED AND SOFT; SOME PORTIONS EXUDE A TAN CREAMY SEMISOLID MATERIAL; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2796 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1192 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91253 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 542.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:35 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2797 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1193 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91254 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 396.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:00 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- MULTIPLE DARK RED AREAS, UP TO 3 MM IN FOCAL DIAMETER; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 10 MM IN DIAMETER UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -DILATATION,-MODERATE, DIFFUSE MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; -DILATATION, SINUSOIDAL,-SLIGHT, MULTI- COLLECTED FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS 2798 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1194 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91254 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 396.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:00 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN OFF NEAR BASE -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), THYROID (TY), MAMMARY, FEMALE (MF), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2799 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1195 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91255 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/22/99 STUDY DAY OF DEATH: 513 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 354.6 GRAMS DATE AND TIME OF NECROPSY: 06/22/99 7:55 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; BRAIN (BR) : BRAIN (BR) : WEEK 62; 20 X 30MM; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); AXILLARY REGION-LEFT; WEEK KIDNEY (KD) : KIDNEY (KD) : 64; 10 X 10MM; VERIFIED AT NECROPSY: -LARGE PELVIS(ES); BILATERAL -CAST, PROTEINACEOUS,-MINIMAL, MULTI- OBSERVED FOCAL >SKIN & PELAGE; SORE/SCAB; AXILLARY -DILATATION, PELVIC,-PRESENT REGION-LEFT; VERIFIED AT NECROPSY: ADRENAL, CORTEX (AC) : OBSERVED -ANGIECTASIS,-SLIGHT, MULTI-FOCAL >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL AT NECROPSY: NOT APPLICABLE LN, MESENTERIC (MS) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -EDEMA,-MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE -INFILTRATE, MACROPHAGE, PIGMENTED,- >APPEARANCE; HUNCHED; VERIFIED AT MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE THYMUS (TH) : >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED -CYST,-PRESENT AT NECROPSY: NOT APPLICABLE THYROID (TY) : >RESPIRATION; LABORED; VERIFIED AT -CYST, ULTIMOBRANCHIAL,-PRESENT NECROPSY: NOT APPLICABLE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 7 X 6 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL VASCULAR AND SOFT -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : JEJUNUM (JE) : >NOTE:>TRIMMING COMMENT: JEJUNUM >TISSUE MISSING MISSING FROM WET TISSUE 2800 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1196 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91255 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/22/99 STUDY DAY OF DEATH: 513 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 354.6 GRAMS DATE AND TIME OF NECROPSY: 06/22/99 7:55 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ RECTUM (RE) : -PARASITISM,-PRESENT SKIN (SK) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL -ULCERATION,-SLIGHT, FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 36 X 28 X 23 MM, TAN, -M-CARCINOMA,- 2 NEOPLASMS PRESENT RED, LOBULATED, CYSTIC AND FIRM; ON SECTION, EXUDES A CLEAR YELLOW FLUID; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 32 X 19 X 9 MM, TAN, RED, VASCULAR AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; OVERLYING SKIN HAS A TAN MOIST ABRADED AREA, 8 X 4 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (SEE AXILLARY) UTERUS (UT) : -DILATATION,-MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS 6 X 4 X 1 -HYPERPLASIA, LYMPHOCYTIC,-MINIMAL, MM (CAP 20) MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 2801 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1197 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91255 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/22/99 STUDY DAY OF DEATH: 513 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 354.6 GRAMS DATE AND TIME OF NECROPSY: 06/22/99 7:55 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2802 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1198 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91256 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 379.0 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 12:53 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL; WEEK 90; BRAIN (BR) : BRAIN (BR) : 40 X 30MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-SLIGHT, MULTI- FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 8 MM IN DIAMETER (RIGHT -ANGIECTASIS,-MODERATE, MULTI-FOCAL IS 5 MM IN DIAMETER) -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID >UNREMARKABLE FILLED CYST, 2 MM IN DIAMETER PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL TAN, GRAY, AND FIRM -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); PERINEAL REGION: SINGLE -GALACTOCELE,-MODERATE, MULTI-FOCAL MASS, 87 X 50 X 28 MM, RED, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- DARK BROWN, TAN, GRAY, LOBULATED, FOCAL VASCULAR AND FIRM; ON CUT SECTION -M-CARCINOMA,-NEOPLASM PRESENT EXUDES A DARK BROWN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED 2803 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1199 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91256 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 379.0 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 12:53 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; LEFT TARSAL JOINT: 13 X 10 X -EXOSTOSES,-MODERATE, MULTI-FOCAL 10 MM (RIGHT IS 10 X 8 X 6 MM); -FIBROSIS,-MODERATE, MULTI-FOCAL COLLECTED LN, POPLITEAL (PP) : LN, POPLITEAL (PP) : -LARGE; LEFT: UP TO 8 X 6 X 3 MM -DILATATION, SINUSOIDAL,-MODERATE, (RIGHT IS UP TO 5 X 3 X 2 MM); APPEARS MULTI-FOCAL CYSTIC (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2804 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1200 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91256 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 379.0 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 12:53 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LUNG (LU), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2805 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1201 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91257 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 219.4 GRAMS DATE AND TIME OF NECROPSY: 08/27/99 8:43 PROSECTOR: DAVID SCHUETTE RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYE-LEFT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: OBSERVED LIVER (LI) : >APPEARANCE; MALOCCLUSION; VERIFIED AT -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- NECROPSY: OBSERVED FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >APPEARANCE; HUNCHED; VERIFIED AT SPLEEN (SP) : NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-MINIMAL, MULTI- >SKIN & PELAGE; YELLOW HAIR COAT; FOCAL VERIFIED AT NECROPSY: NOT OBSERVED ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LIGHT FOCUS(I)/AREA(S); BILATERAL, -ANGIECTASIS,-MINIMAL, MULTI-FOCAL DIFFUSE: MULTIPLE PINPOINT TAN AREAS -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL, FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 8 X 6 X 4 MM, -CYST,-PRESENT TAN, DARK RED, AND SOFT; COLLECTED -HEMORRHAGE,-SLIGHT, MULTI-FOCAL INTACT WITH BRAIN -PIGMENT,-MINIMAL, FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2806 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1202 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91257 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 219.4 GRAMS DATE AND TIME OF NECROPSY: 08/27/99 8:43 PROSECTOR: DAVID SCHUETTE RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN; -GALACTOCELE,-MINIMAL, MULTI-FOCAL COLLECTED ON ROUTINE SECTION -MINERALIZATION,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MEDIASTINAL (ML) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: MOIST BROWN MATERIAL -MALOCCLUSION; BILATERAL, UPPER INCISORS -ANIMAL THIN -------------------------------------------- 2807 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1203 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91257 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 219.4 GRAMS DATE AND TIME OF NECROPSY: 08/27/99 8:43 PROSECTOR: DAVID SCHUETTE RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2808 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1204 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91258 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 283.6 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 15:38 PROSECTOR: TERRI L. WILLIAMS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 79; 40 X 30MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK KIDNEY (KD) : 71; 45 X 50MM; VERIFIED AT NECROPSY: -CAST, PROTEINACEOUS,-MINIMAL, FOCAL OBSERVED ADRENAL, CORTEX (AC) : >APPEARANCE; HUNCHED; VERIFIED AT -ANGIECTASIS,-SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE -HEMORRHAGE,-SLIGHT, MULTI-FOCAL >APPEARANCE; THIN; VERIFIED AT OVARY (OV) : OVARY (OV) : NECROPSY: OBSERVED -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT FILLED CYST, 5 MM IN DIAMETER NECROPSY: NOT APPLICABLE THYROID (TY) : >SKIN & PELAGE; YELLOW HAIR COAT; -HYPERPLASIA, C-CELL,-SLIGHT, FOCAL VERIFIED AT NECROPSY: OBSERVED PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- GRAY AND SOFT FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-SLIGHT, MULTI-FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI- FOCAL 2809 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1205 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91258 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 283.6 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 15:38 PROSECTOR: TERRI L. WILLIAMS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -MINERALIZATION,-SLIGHT, MULTI-FOCAL SINGLE MASS, 30 X 30 X 25 MM, RED, -B-ADENOMA,-NEOPLASM PRESENT DARK RED, BROWN, TAN, FIRM AND -M-CARCINOMA,-NEOPLASM PRESENT LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN AND CREAMY FLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER; COLLECTED -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 45 X 45 X 35 MM, TAN, BROWN, VASCULAR AND SOFT; ON SECTION EXUDE A TAN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: RED -ANIMAL THIN -------------------------------------------- 2810 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1206 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91258 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 283.6 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 15:38 PROSECTOR: TERRI L. WILLIAMS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2811 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1207 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91259 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/08/99 STUDY DAY OF DEATH: 438 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/08/99 13:02 PROSECTOR: JOHN S. HALFORD RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYE-RIGHT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC -FIBROSIS,-MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE SURFACE OF RIGHT MEDIAN LOBE: SINGLE -HEMORRHAGE,-MINIMAL, MULTI-FOCAL >BEHAVIOR; ATAXIC; VERIFIED AT GRAY AND TAN AREA, 7 X 5 MM; COLLECTED NECROPSY: NOT APPLICABLE ON ROUTINE SECTION >APPEARANCE; HEAD TILT; RIGHT; VERIFIED KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-SLIGHT, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 14 X 10 X 9 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, TAN, AND SOFT ILEUM (IL) : >AUTOLYTIC VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE GREEN, MUCOID SEMIFLUID CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -ANIMAL THIN 2812 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1208 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91259 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/08/99 STUDY DAY OF DEATH: 438 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/08/99 13:02 PROSECTOR: JOHN S. HALFORD RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -AUTOLYSIS-SEVERE; INVOLVING JEJUNUM AND ILEUM -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2813 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1209 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91260 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 312.7 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 8:47 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: OBSERVED -HEMORRHAGE,-MODERATE, MULTI-FOCAL >SKIN & PELAGE; RED HAIR COAT; HEAD- -PIGMENT,-MINIMAL, MULTI-FOCAL ENTIRE; VERIFIED AT NECROPSY: NOT LIVER (LI) : OBSERVED -HEMORRHAGE,-MINIMAL, FOCAL >APPEARANCE; THIN; VERIFIED AT -NECROSIS, HEPATOCELLULAR,-SLIGHT, NECROPSY: NOT OBSERVED FOCAL >SKIN & PELAGE; BROWN HAIR COAT; KIDNEY (KD) : PERINEAL AREA; VERIFIED AT NECROPSY: -CAST, PROTEINACEOUS,-MINIMAL, MULTI- OBSERVED FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -MINERALIZATION, PELVIC,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >RESPIRATION; LABORED; VERIFIED AT PANCREAS (PA) : NECROPSY: NOT APPLICABLE -PIGMENT,-SLIGHT, MULTI-FOCAL >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : AT NECROPSY: NOT APPLICABLE -LARGE; RIGHT: 7 MM IN DIAMETER (FOR -ANGIECTASIS,-MODERATELY SEVERE, MULTI- COMPARISON, LEFT IS 5 MM IN DIAMETER) FOCAL -HEMORRHAGE,-MODERATELY SEVERE, MULTI- FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID >UNREMARKABLE FILLED CYST, 5 MM IN DIAMETER 2814 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1210 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91260 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 312.7 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 8:47 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 12 X 9 X 8 MM, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- DARK RED, TAN AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BROWN AND DRY -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED AND DRY -------------------------------------------- 2815 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1211 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91260 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 312.7 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 8:47 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2816 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1212 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91261 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 689 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/15/99 10:58 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; HEAD- EYE (EY) : EYE (EY) : CRANIAL; VERIFIED AT NECROPSY: -OPAQUE CORNEA; LEFT >UNREMARKABLE OBSERVED BRAIN (BR) : BRAIN (BR) : >MASS(ES); AXILLARY REGION-RIGHT; WEEK -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT 87; 60 X 70MM; VERIFIED AT NECROPSY: -DILATATION, VENTRICULAR,-PRESENT OBSERVED -HEMORRHAGE,-MODERATE, MULTI-FOCAL >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- SLIGHT, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, FOCAL -THROMBUS,-MINIMAL, FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 6 MM IN DIAMETER PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 9 X 7 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL GREY, LOBULATED AND SOFT -B-ADENOMA,-NEOPLASM PRESENT 2817 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1213 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91261 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 689 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/15/99 10:58 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ JEJUNUM (JE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 65 X 45 X 33 MM, RED, DARK RED, TAN, GREY, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 21) CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: DRY, RED MATERIAL -ANIMAL THIN -------------------------------------------- 2818 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1214 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91261 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 689 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/15/99 10:58 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2819 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1215 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91262 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 298.9 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 14:57 PROSECTOR: JILL PAUS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-THORACIC; WEEK 81; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT -HEMORRHAGE,-MODERATE, FOCAL NECROPSY: OBSERVED KIDNEY (KD) : KIDNEY (KD) : >SKIN & PELAGE; RED HAIR COAT; HEAD- -LARGE PELVIS(ES); RIGHT -DILATATION, PELVIC,-PRESENT ENTIRE; VERIFIED AT NECROPSY: NOT >NOTE:>RIGHT KIDNEY OBSERVED ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : >APPEARANCE; HUNCHED; VERIFIED AT -DIFFUSELY LIGHT; BILATERAL: TAN -DEGENERATION, CYSTIC, CORTICAL,- NECROPSY: NOT APPLICABLE MODERATELY SEVERE, FOCAL >SKIN & PELAGE; YELLOW HAIR COAT; -HEMORRHAGE,-MODERATE, FOCAL VERIFIED AT NECROPSY: OBSERVED LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : >EXCRETION; NO FECES; VERIFIED AT -DIFFUSELY RED; DARK RED -INFILTRATE, MACROPHAGE, PIGMENTED,- NECROPSY: NOT APPLICABLE SLIGHT, MULTI-FOCAL >APPEARANCE; DEHYDRATED; VERIFIED AT HEART (HT) : NECROPSY: NOT APPLICABLE -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 5 MM IN DIAMETER, DARK RED, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL TAN, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL CERVIX (CV) : -M-SARCOMA, ENDOMETRIAL STROMAL,- NEOPLASM PRESENT 2820 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1216 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91262 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 298.9 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 14:57 PROSECTOR: JILL PAUS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CERVIX (CV) : -MASS(ES); INVOLVING ENTIRE CERVIX: SINGLE MASS, 50 X 45 X 30 MM, RED, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE LUMBAR) VAGINA (VA) : VAGINA (VA) : -MASS(ES); MUCOSA: SINGLE RED, TAN, -I-ENDOMETRIAL STROMAL SARCOMA,- GELATINOUS, AND PEDUNCULATED MASS, 20 NEOPLASM PRESENT X 12 X 6 MM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE LUMBAR) CAUSE OF DEATH (CD) : -ENDOMETRIAL STROMAL SARCOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: LEFT IS 18 X 14 X -N-ENDOMETRIAL STROMAL SARCOMA,- 10 MM, RIGHT IS 50 X 40 X 40 MM; BOTH NEOPLASM PRESENT APPEAR CYSTIC (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED DRY MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: YELLOW AND MOIST -ANIMAL THIN -------------------------------------------- 2821 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1217 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91262 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 298.9 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 14:57 PROSECTOR: JILL PAUS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2822 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1218 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91263 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/07/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 272.5 GRAMS DATE AND TIME OF NECROPSY: 06/07/99 15:10 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 22; 40 X 40MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-SLIGHT, MULTI- >BEHAVIOR; HYPOACTIVE; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE PANCREAS (PA) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -ATROPHY, ACINAR,-MINIMAL, FOCAL NECROPSY: OBSERVED ADRENAL, CORTEX (AC) : >SKIN & PELAGE; BROWN HAIR COAT; -DEGENERATION, CYSTIC, CORTICAL,- PERINEAL AREA; VERIFIED AT NECROPSY: MODERATE, FOCAL OBSERVED -B-ADENOMA,-NEOPLASM PRESENT >EXCRETION; FEW FECES; VERIFIED AT LUNG (LU) : NECROPSY: NOT APPLICABLE -HEMORRHAGE,-MINIMAL, FOCAL >BEHAVIOR; ATAXIC; VERIFIED AT THYROID (TY) : NECROPSY: NOT APPLICABLE -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 9 X 6 X 5 MM, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- DARK RED AND SOFT FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -PIGMENT,-MODERATE, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT 2823 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1219 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91263 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/07/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 272.5 GRAMS DATE AND TIME OF NECROPSY: 06/07/99 15:10 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 45 X 35 X 15 MM, RED, DARK RED, GRAY, TAN AND SOFT; CUT SURFACE EXUDES A DARK GREEN FLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW, BROWN AND DRY -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -------------------------------------------- 2824 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1220 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91263 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/07/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 272.5 GRAMS DATE AND TIME OF NECROPSY: 06/07/99 15:10 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2825 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1221 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91264 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 398.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:38 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 93; 20 X 20MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); AXILLARY REGION-LEFT; WEEK LIVER (LI) : 97; 25 X 25MM; VERIFIED AT NECROPSY: -VACUOLATION, HEPATOCELLULAR,-MINIMAL, OBSERVED MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI- FOCAL LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -LARGE; UP TO 8 MM IN DIAMETER >UNREMARKABLE THYROID (TY) : THYROID (TY) : -LARGE; RIGHT: 8 X 4 X 2 MM (LEFT IS 5 -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM X 3 X 1 MM) PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 8 X 8 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL TAN AND FIRM -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-MINIMAL, MULTI-FOCAL SINGLE MASS, 35 X 30 X 18 MM, TAN, -B-ADENOMA,- 2 NEOPLASMS PRESENT BROWN, LOBULATED, VASCULAR AND SOFT; ON SECTION EXUDES A BROWN FLUID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) 2826 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1222 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91264 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 398.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:38 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 27 X 27 X 15 MM, TAN, RED, DARK BROWN, LOBULATED AND FIRM; ON CUT SURFACE EXUDES A DARK RED FLUID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) UTERUS (UT) : UTERUS (UT) : -CYST(S); LEFT, WITHIN WALLS: TWO -DILATATION,-MODERATE, FOCAL CLEAR FLUID FILLED CYSTS, BOTH ARE 5 X 4 X 4 MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -HEMORRHAGE,-MODERATE, MULTI-FOCAL (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2827 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1223 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91264 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 398.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:38 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), URINARY BLADDER (UB), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2828 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1224 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91265 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/09/99 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 310.4 GRAMS DATE AND TIME OF NECROPSY: 11/09/99 7:24 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >SKIN & PELAGE; YELLOW HAIR COAT; -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- VERIFIED AT NECROPSY: OBSERVED FOCAL >APPEARANCE; THIN; VERIFIED AT -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NECROPSY: OBSERVED FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -VACUOLATION, HEPATOCELLULAR,-SLIGHT, NECROPSY: NOT APPLICABLE MULTI-FOCAL >BEHAVIOR; ATAXIC; VERIFIED AT PANCREAS (PA) : NECROPSY: NOT APPLICABLE -ATROPHY, ACINAR,-MODERATE, MULTI-FOCAL >APPEARANCE; HUNCHED; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: NOT APPLICABLE -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 10 X 6 MM, RED, DARK -CYST,-PRESENT RED, TAN AND SOFT -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL STOMACH, GL (ST) : -EDEMA,-MODERATE, MULTI-FOCAL 2829 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1225 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91265 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/09/99 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 310.4 GRAMS DATE AND TIME OF NECROPSY: 11/09/99 7:24 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE GREEN MUCOID SEMIFLUID MATERIAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: YELLOW MOIST MATERIAL; POSSIBLY CONSISTENT WITH YELLOW HAIRCOAT AS NOTED IN CLINICAL OBSERVATIONS -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -ANIMAL THIN -------------------------------------------- 2830 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1226 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91265 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/09/99 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 310.4 GRAMS DATE AND TIME OF NECROPSY: 11/09/99 7:24 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2831 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1227 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91266 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/18/00 STUDY DAY OF DEATH: 723 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 309.0 GRAMS DATE AND TIME OF NECROPSY: 01/18/00 11:30 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : LEFT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); INGUINAL REGION-RIGHT; WEEK -DILATATION, VENTRICULAR,-PRESENT 89; 20 X 20MM; VERIFIED AT NECROPSY: -HEMORRHAGE,-MODERATE, MULTI-FOCAL OBSERVED LIVER (LI) : >MASS(ES); AXILLARY REGION-RIGHT; WEEK -VACUOLATION, HEPATOCELLULAR,- 97; 10 X 10MM; VERIFIED AT NECROPSY: MODERATELY SEVERE, DIFFUSE OBSERVED KIDNEY (KD) : >APPEARANCE; THIN; VERIFIED AT -MINERALIZATION, PELVIC,-MINIMAL, MULTI- NECROPSY: OBSERVED FOCAL >EXCRETION; NO FECES; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE MODERATE, MULTI-FOCAL >SKIN & PELAGE; BROWN HAIR COAT; -PYELONEPHRITIS,-SLIGHT, MULTI-FOCAL VENTRAL-ABDOMINAL; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: OBSERVED -HYPERTROPHY, CORTICAL CELL,-MODERATE, >SKIN & PELAGE; BLACK SKIN; VENTRAL- MULTI-FOCAL ABDOMINAL-LEFT; VERIFIED AT NECROPSY: -VACUOLATION,-MINIMAL, FOCAL NOT OBSERVED LN, MESENTERIC (MS) : >APPEARANCE; HEAD TILT; RIGHT; VERIFIED -INFILTRATE, MACROPHAGE, PIGMENTED,- AT NECROPSY: NOT APPLICABLE MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 10 X 4 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL LOBULATED AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MODERATE, FOCAL 2832 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1228 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91266 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/18/00 STUDY DAY OF DEATH: 723 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 309.0 GRAMS DATE AND TIME OF NECROPSY: 01/18/00 11:30 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE MOIST TAN ULCERATED AREA, 7 X 4 MM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL SINGLE MASS, 40 X 30 X 10 MM, TAN, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL GREEN, RED, LOBULATED AND FIRM; SAME -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE, ON CUT SURFACE; COLLECTED INTACT; MULTI-FOCAL REGIONAL LN (INGUINAL) NOT IDENTIFIED -MINERALIZATION,-MINIMAL, MULTI-FOCAL -MASS #2; RIGHT AXILLARY REGION: -PIGMENT,-SLIGHT, MULTI-FOCAL SINGLE MASS, 25 X 15 X 15 MM, TAN, RED -M-CARCINOMA,- 2 NEOPLASMS PRESENT AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 21) CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HIND FOOT: -FIBROSIS,-MODERATELY SEVERE, FOCAL SINGLE DRY BROWN CRUSTED AREA, 5 MM IN -INFLAMMATION, CHRONIC ACTIVE,-SLIGHT, DIAMETER; COLLECTED MULTI-FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2833 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1229 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91266 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/18/00 STUDY DAY OF DEATH: 723 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 309.0 GRAMS DATE AND TIME OF NECROPSY: 01/18/00 11:30 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2834 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1230 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91267 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/04/99 STUDY DAY OF DEATH: 464 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 404.8 GRAMS DATE AND TIME OF NECROPSY: 05/04/99 14:08 PROSECTOR: MICHELE MARGGI RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; SPLEEN (SP) : WEEK 38; 25 X 35MM; VERIFIED AT -HEMATOPOIESIS, EXTRAMEDULLARY, NECROPSY: OBSERVED INCREASED,-MINIMAL, MULTI-FOCAL >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK ADRENAL, CORTEX (AC) : 46; 70 X 70MM; VERIFIED AT NECROPSY: -DEGENERATION, CYSTIC, CORTICAL,- OBSERVED MODERATE, FOCAL >SKIN & PELAGE; SORE/SCAB; VENTRAL- -HEMORRHAGE,-SLIGHT, FOCAL ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: PITUITARY (PI) : PITUITARY (PI) : OBSERVED -DARK FOCUS(I)/AREA(S); MID ASPECT: -CYST,-PRESENT SINGLE DARK BROWN AREA, 3 X 2 MM -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL SKIN (SK) : -ULCERATION,-SEVERE, FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT CERVICAL REGION: -GALACTOCELE,-MINIMAL, MULTI-FOCAL SINGLE MASS, 75 X 70 X 30 MM, RED, -B-FIBROADENOMA,-NEOPLASM PRESENT TAN, FIRM AND LOBULATED; SAME ON CUT -M-CARCINOMA,-NEOPLASM PRESENT SURFACE (COLLECTED); REGIONAL LN (MANDIBULAR) NORMAL (CAP 20) 2835 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1231 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91267 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/04/99 STUDY DAY OF DEATH: 464 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 404.8 GRAMS DATE AND TIME OF NECROPSY: 05/04/99 14:08 PROSECTOR: MICHELE MARGGI RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #2; RIGHT ABDOMINAL REGION: SINGLE MASS, 45 X 30 X 15 MM, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE (COLLECTED); REGIONAL LN (INGUINAL) NORMAL (CAP 21); SKIN OVERLYING MASS HAS A SINGLE TAN, ABRADED AREA, 45 X 20 MM; COLLECTED INTACT WITH MASS CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2836 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1232 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91267 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/04/99 STUDY DAY OF DEATH: 464 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 404.8 GRAMS DATE AND TIME OF NECROPSY: 05/04/99 14:08 PROSECTOR: MICHELE MARGGI RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), HEMATO NEOPLASIA (HN), LN, INGUINAL (IN) ------------------------------------------------------------------------------------------------------------------------------------ 2837 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1233 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91268 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 618.5 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:04 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); HEAD-MAXILLARY-LEFT; WEEK LIVER (LI) : LIVER (LI) : 80; 30 X 30MM; VERIFIED AT NECROPSY: -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC >UNREMARKABLE OBSERVED SURFACE OF LEFT LATERAL LOBE: SINGLE >EYES; DISCOLORED/DARK RED; EYE-LEFT; DARK RED AREA, 7 MM IN DIAMETER; VERIFIED AT NECROPSY: NOT APPLICABLE COLLECTED ON ROUTINE SECTION >SKIN & PELAGE; SORE/SCAB; PAW-HIND- KIDNEY (KD) : LEFT; VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT LATERAL -CYST,-PRESENT ASPECT: SINGLE DARK RED AREA, 2 MM IN -HEMORRHAGE,-SLIGHT, MULTI-FOCAL DIAMETER -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI- FOCAL 2838 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1234 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91268 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 618.5 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:04 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : SKIN (SK) : -MASS(ES); LEFT LATERAL CERVICAL -B-KERATOACANTHOMA,-NEOPLASM PRESENT REGION, EXTENDING ALONG LEFT MANDIBLE: SINGLE MASS, 40 X 35 X 30 MM, RED, DARK RED, TAN AND SOFT; ON SECTION EXUDES A TAN SEMIFLUID; COLLECTED; REGIONAL LN (SEE MANDIBULAR) MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -KERATOACANTHOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; -HEMORRHAGE,-MINIMAL, MULTI-FOCAL COLLECTED FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: -FIBROSIS,-MODERATE, FOCAL SINGLE DARK RED CRUSTED AREA, 5 MM IN -INFLAMMATION, ACUTE,-MODERATE, FOCAL DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL OBESE GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY -------------------------------------------- 2839 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1235 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91268 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 618.5 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:04 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2840 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1236 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91269 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/26/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 295.3 GRAMS DATE AND TIME OF NECROPSY: 08/26/99 14:00 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; YELLOW HAIR COAT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; NO FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR,-MINIMAL, >EYES; RED DISCHARGE; EYE-LEFT; MULTI-FOCAL VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : KIDNEY (KD) : >DISCHARGE; NASAL; RED IN COLOR; -GRANULAR MATERIAL; LEFT, WITHIN -CAST, PROTEINACEOUS,-MINIMAL, FOCAL VERIFIED AT NECROPSY: OBSERVED PELVIS: MULTIPLE TAN PINPOINT GRANULES -MINERALIZATION, PELVIC,-MODERATE, >APPEARANCE; THIN; VERIFIED AT MULTI-FOCAL NECROPSY: OBSERVED SPLEEN (SP) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -PIGMENT, INCREASED,-MODERATE, MULTI- NECROPSY: NOT APPLICABLE FOCAL >APPEARANCE; HEAD TILT; LEFT; VERIFIED ADRENAL, CORTEX (AC) : AT NECROPSY: NOT APPLICABLE -DEGENERATION, CYSTIC, CORTICAL,- MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 6 MM, RED, TAN AND -B-ADENOMA,-NEOPLASM PRESENT SOFT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2841 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1237 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91269 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/26/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 295.3 GRAMS DATE AND TIME OF NECROPSY: 08/26/99 14:00 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DARK BROWN AND DRY MATERIAL -DISCHARGE-NASAL; DARK BROWN AND DRY MATERIAL -------------------------------------------- 2842 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1238 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91269 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/26/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 295.3 GRAMS DATE AND TIME OF NECROPSY: 08/26/99 14:00 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2843 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1239 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91270 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/08/99 STUDY DAY OF DEATH: 407 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: 226.6 GRAMS DATE AND TIME OF NECROPSY: 03/08/99 12:58 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; RED HAIR COAT; VENTRAL- -DILATATION, VENTRICULAR,-PRESENT ABDOMINAL; VERIFIED AT NECROPSY: NOT KIDNEY (KD) : OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >APPEARANCE; HEAD TILT; LEFT; VERIFIED FOCAL AT NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MINIMAL, MULTI- >RESPIRATION; LABORED; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -DARK FOCUS(I)/AREA(S); BILATERAL, -ANGIECTASIS,-SLIGHT, MULTI-FOCAL WITHIN CORTEX: MULTIPLE BROWN AREAS ON -DEGENERATION, CYSTIC, CORTICAL,- EACH, EACH IS UP TO 2 MM IN DIAMETER MODERATELY SEVERE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 8 X 5 MM, RED, DARK -HEMORRHAGE,-MINIMAL, MULTI-FOCAL RED, TAN, GREY AND SOFT -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: -GALACTOCELE,-MODERATE, MULTI-FOCAL MULTIPLE TAN CREAMY FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER (CAP 20) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION 2844 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1240 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91270 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/08/99 STUDY DAY OF DEATH: 407 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: 226.6 GRAMS DATE AND TIME OF NECROPSY: 03/08/99 12:58 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED -DISCHARGE-NASAL; BILATERAL: RED -ANIMAL THIN -------------------------------------------- 2845 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1241 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91270 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/08/99 STUDY DAY OF DEATH: 407 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: 226.6 GRAMS DATE AND TIME OF NECROPSY: 03/08/99 12:58 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2846 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1242 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91271 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/09/99 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 314.5 GRAMS DATE AND TIME OF NECROPSY: 11/09/99 13:36 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 84; 35 X 50MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); AXILLARY REGION-LEFT; WEEK KIDNEY (KD) : 84; 30 X 30MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MINIMAL, MULTI- OBSERVED FOCAL >MASS(ES); AXILLARY REGION-RIGHT; WEEK ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : 89; VERIFIED AT NECROPSY: OBSERVED -LARGE; BILATERAL: RIGHT IS 7 X 5 X 4 -ANGIECTASIS,-MODERATELY SEVERE, FOCAL >APPEARANCE; HUNCHED; VERIFIED AT MM, LEFT IS 6 X 5 X 3 MM -DEGENERATION, CYSTIC, CORTICAL,- NECROPSY: NOT APPLICABLE MODERATE, FOCAL >EYES; RED DISCHARGE; EYE-LEFT; -HEMORRHAGE,-MINIMAL, MULTI-FOCAL VERIFIED AT NECROPSY: OBSERVED -PIGMENT,-MINIMAL, FOCAL >APPEARANCE; THIN; VERIFIED AT -THROMBUS,-MINIMAL, FOCAL NECROPSY: OBSERVED PITUITARY (PI) : PITUITARY (PI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -MASS(ES); 11 X 10 X 7 MM, RED, DARK -HEMORRHAGE,-SEVERE, MULTI-FOCAL NECROPSY: NOT APPLICABLE RED, TAN, LOBULATED AND SOFT -B-ADENOMA,-NEOPLASM PRESENT >SKIN & PELAGE; YELLOW HAIR COAT; STOMACH, NONGL (SU) : VERIFIED AT NECROPSY: OBSERVED -EDEMA,-MODERATE, FOCAL >DISCHARGE; GENITAL; RED IN COLOR; MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : VERIFIED AT NECROPSY: OBSERVED -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-MODERATELY SEVERE, MULTI- SINGLE MASS, 11 X 10 X 6 MM, RED, TAN FOCAL AND FIRM; SAME ON CUT SURFACE (CAP -B-FIBROADENOMA,- 4 NEOPLASMS PRESENT 21); REGIONAL LN (AXILLARY) NORMAL (CAP 22) 2847 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1243 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91271 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/09/99 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 314.5 GRAMS DATE AND TIME OF NECROPSY: 11/09/99 13:36 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 45 X 38 X 26 MM, RED, TAN, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (AXILLARY) NORMAL (CAP 22) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #3; LEFT INGUINAL REGION: SINGLE MASS, 40 X 30 X 16 MM, RED, TAN, LOBULATED, SOME AREAS ARE SOFT AND SOME AREAS ARE FIRM; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A GREEN, CREAMY SEMIFLUID (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #4; LEFT LATERAL ABDOMINAL REGION: SINGLE MASS, 25 X 18 X 5 MM, RED, TAN, DARK GREEN, LOBULATED AND SOFT; ON SECTION EXUDES A GREEN, CREAMY SEMIFLUID (CAP 25); REGIONAL LN (INGUINAL) NOT IDENTIFIED VAGINA (VA) : VAGINA (VA) : -MASS(ES); WITHIN LUMEN: SINGLE MASS, -M-CARCINOMA, SQUAMOUS CELL,-NEOPLASM 12 X 10 X 6 MM, RED, TAN AND FIRM; ON PRESENT SECTION EXUDES A CLEAR FLUID; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 26) CAUSE OF DEATH (CD) : -SQUAMOUS CELL CARCINOMA,-PRESENT 2848 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1244 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91271 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/09/99 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 314.5 GRAMS DATE AND TIME OF NECROPSY: 11/09/99 13:36 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: MOIST RED AND YELLOW MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -ANIMAL THIN -------------------------------------------- 2849 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1245 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91271 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/09/99 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 314.5 GRAMS DATE AND TIME OF NECROPSY: 11/09/99 13:36 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2850 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1246 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91272 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 206.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:06 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.4750 3.6269 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2851 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1247 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91272 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 206.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:06 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2852 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1248 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91273 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 354.7 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 13:13 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 76; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; HEAD TILT; RIGHT; VERIFIED KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 7 X 9 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL SOFT, RED, DARK RED AND TAN -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 7 X 10 X 14 MM, FIRM, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL RED, DARK RED, TAN AND LOBULATED; SAME -INFLAMMATION, ACUTE,-SLIGHT, FOCAL ON CUT SURFACE (CAP 21); REGIONAL LN -B-FIBROADENOMA,-NEOPLASM PRESENT (INGUINAL) NOT IDENTIFIED 2853 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1249 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91273 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 354.7 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 13:13 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: FIRM AND TAN; COLLECTED WITH ROUTINE SECTION CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MEDIASTINAL (ML) : -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DRY RED MATERIAL -------------------------------------------- 2854 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1250 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91273 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 354.7 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 13:13 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2855 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1251 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91274 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/30/99 STUDY DAY OF DEATH: 490 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/30/99 13:58 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI- FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL SPINAL CORD (SC) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 11 X 8 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- GREY, TAN, LOBULATED, CYSTIC AND SOFT; FOCAL ON SECTION EXUDES A CLEAR FLUID -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL 2856 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1252 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91274 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/30/99 STUDY DAY OF DEATH: 490 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/30/99 13:58 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; RIGHT: DARK -DILATATION,-MODERATE, FOCAL RED FLUID -B-POLYP, ENDOMETRIAL, STROMAL,- NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: DARK RED AND TAN; >UNREMARKABLE COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: MOIST, YELLOW MATERIAL -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -------------------------------------------- 2857 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1253 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91274 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/30/99 STUDY DAY OF DEATH: 490 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/30/99 13:58 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2858 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1254 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91275 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/20/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 296.5 GRAMS DATE AND TIME OF NECROPSY: 05/20/99 9:12 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 58; 25 X 25MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; ROUGH HAIR COAT; KIDNEY (KD) : VERIFIED AT NECROPSY: NOT APPLICABLE -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >EYES; RED DISCHARGE; EYE-LEFT; FOCAL VERIFIED AT NECROPSY: OBSERVED ADRENAL, CORTEX (AC) : >APPEARANCE; THIN; VERIFIED AT -ANGIECTASIS,-MODERATE, MULTI-FOCAL NECROPSY: OBSERVED -HEMORRHAGE,-MODERATE, MULTI-FOCAL >EXCRETION; FEW FECES; VERIFIED AT OVARY (OV) : NECROPSY: NOT APPLICABLE -CYST,-PRESENT LUNG (LU) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL MUSCLE, SKELETAL (SM) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 10 X 8 MM, DARK RED, -HEMORRHAGE,-SEVERE, FOCAL GRAY, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT 2859 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1255 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91275 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/20/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 296.5 GRAMS DATE AND TIME OF NECROPSY: 05/20/99 9:12 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); LEFT SHOULDER REGION: SINGLE -GALACTOCELE,-MODERATELY SEVERE, MULTI- CYST, 20 MM IN DIAMETER, FILLED WITH A FOCAL DARK BROWN, TENACIOUS SEMIFLUID AND -INFLAMMATION, ACUTE,-MODERATE, FOCAL BROWN, FRIABLE SEMISOLID MATERIAL (CAP -MINERALIZATION,-MODERATE, MULTI-FOCAL 20); POSSIBLY CONSISTENT WITH MASS -PIGMENT,-MINIMAL, MULTI-FOCAL NOTED IN CLINICAL OBSERVATIONS -B-FIBROADENOMA,-NEOPLASM PRESENT -THICKENED; ALL REGIONS, DIFFUSE: TAN, FIRM, AND CYSTIC (CAP 21) CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT TAIL (TI) : TAIL (TI) : -CRUSTED AREA(S); ALONG ENTIRE LENGTH: -EROSION,-SLIGHT, MULTI-FOCAL MULTIPLE BROWN, DRY, CRUSTED AREAS, UP -INFLAMMATION, ACUTE,-MODERATE, MULTI- TO 5 X 3 MM; COLLECTED FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED AND DRY -ANIMAL THIN -------------------------------------------- 2860 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1256 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91275 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/20/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 296.5 GRAMS DATE AND TIME OF NECROPSY: 05/20/99 9:12 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2861 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1257 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91276 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 410.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:44 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; BRAIN (BR) : BRAIN (BR) : WEEK 62; 30 X 40MM; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); VENTRAL-ABDOMINAL; WEEK 72; KIDNEY (KD) : 40 X 45MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MINIMAL, MULTI- OBSERVED FOCAL >SKIN & PELAGE; YELLOW HAIR COAT; ADRENAL, CORTEX (AC) : VERIFIED AT NECROPSY: OBSERVED -DEGENERATION, CYSTIC, CORTICAL,- >MASS(ES); AXILLARY REGION-LEFT; WEEK MODERATELY SEVERE, MULTI-FOCAL 82; 30 X 20MM; VERIFIED AT NECROPSY: -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OBSERVED THYMUS (TH) : >MASS(ES); DORSAL-CERVICAL-LEFT; WEEK -CYST,-PRESENT 82; 70 X 60MM; VERIFIED AT NECROPSY: LUNG (LU) : OBSERVED -INFILTRATE, MACROPHAGE, PIGMENTED,- >MASS(ES); INGUINAL REGION-LEFT; WEEK SLIGHT, FOCAL 88; 30 X 20MM; VERIFIED AT NECROPSY: PITUITARY (PI) : PITUITARY (PI) : OBSERVED -MASS(ES); 10 X 6 X 6 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- >MASS(ES); AXILLARY REGION-RIGHT; WEEK TAN AND FIRM FOCAL 93; VERIFIED AT NECROPSY: OBSERVED -B-ADENOMA,-NEOPLASM PRESENT >SKIN & PELAGE; BLUE SKIN; VENTRAL- SKIN (SK) : ABDOMINAL; VERIFIED AT NECROPSY: NOT -INFLAMMATION, ACUTE,-SLIGHT, FOCAL APPLICABLE -ULCERATION,-SLIGHT, FOCAL >APPEARANCE; MALOCCLUSION; VERIFIED AT MAMMARY, FEMALE (MF) : NECROPSY: NOT OBSERVED -GALACTOCELE,-SEVERE, MULTI-FOCAL 2862 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1258 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91276 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 410.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:44 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >HOUSING,FOOD,WATER & MAINT.; TEETH MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : CUT; VERIFIED AT NECROPSY: NOT -MASS(ES); LEFT AXILLARY REGION: -HEMORRHAGE,-MODERATELY SEVERE, MULTI- APPLICABLE SINGLE MASS, 36 X 24 X 16 MM, TAN, FOCAL >EYES; PALE; EYES; VERIFIED AT RED, BLACK AND SOFT; SAME ON CUT -MINERALIZATION,-MODERATE, MULTI-FOCAL NECROPSY: NOT APPLICABLE SURFACE (CAP 21); REGIONAL LN (SEE -B-FIBROADENOMA,- 3 NEOPLASMS PRESENT >SKIN & PELAGE; SORE/SCAB; LATERAL- AXILLARY) -M-CARCINOMA,- 2 NEOPLASMS PRESENT ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: -CYST(S); ALL REGIONS, DIFFUSE: OBSERVED MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -MASS #2; LEFT AXILLARY, CRANIAL TO MASS #1: SINGLE MASS, 75 X 60 X 25 MM, TAN, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #3; LEFT INGUINAL REGION: SINGLE MASS, 80 X 27 X 19 MM, TAN, BROWN, BLACK AND SOFT; ON SECTION, EXUDES A DARK BROWN SEMIFLUID (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #4; RIGHT AXILLARY REGION: SINGLE MASS, 30 X 20 X 17 MM, TAN, BROWN, LOBULATED AND SOFT; ON SECTION, EXUDES A TAN CREAMY SEMISOLID (CAP 25); REGIONAL LN (SEE AXILLARY) 2863 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1259 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91276 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 410.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:44 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #5; RIGHT INGUINAL REGION: SINGLE MASS, 89 X 54 X 14 MM, RED, TAN, BROWN, LOBULATED, AND SOFT; ON SECTION, EXUDES A DARK BROWN SEMIFLUID (CAP 26); REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN HAS A RED, BLACK, DRY ULCERATED AREA, 20 X 15 MM; COLLECTED INTACT WITH MASS CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; LEFT: 12 X 7 X 2 MM (RIGHT IS -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, 6 X 3 X 1 MM); COLLECTED DIFFUSE LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 22) GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2864 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1260 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91276 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 410.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:44 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2865 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1261 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91277 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/07/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 222.9 GRAMS DATE AND TIME OF NECROPSY: 06/07/99 14:53 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK KIDNEY (KD) : 72; 30 X 50MM; VERIFIED AT NECROPSY: -MINERALIZATION, TUBULAR,-MINIMAL, OBSERVED MULTI-FOCAL >APPEARANCE; THIN; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: OBSERVED MINIMAL, MULTI-FOCAL >APPEARANCE; HUNCHED; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: NOT APPLICABLE -ANGIECTASIS,-MINIMAL, MULTI-FOCAL >EYES; RED DISCHARGE; EYES; VERIFIED AT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL NECROPSY: OBSERVED -VACUOLATION,-MINIMAL, MULTI-FOCAL >SKIN & PELAGE; PALE; ENTIRE BODY; THYMUS (TH) : VERIFIED AT NECROPSY: NOT APPLICABLE -CYST,-PRESENT >SKIN & PELAGE; COLD TO TOUCH; ENTIRE HEART (HT) : BODY; VERIFIED AT NECROPSY: NOT -MINERALIZATION,-MINIMAL, MULTI-FOCAL APPLICABLE PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); MID ASPECT: -HEMORRHAGE,-MODERATE, MULTI-FOCAL SINGLE DARK BROWN AREA, 5 X 2 MM -B-ADENOMA,-NEOPLASM PRESENT -LARGE; 7 X 4 X 3 MM; APPEARS SWOLLEN STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EROSION,-MODERATE, FOCAL MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 -MINERALIZATION,-MODERATE, MULTI-FOCAL MM SKIN (SK) : -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL -ULCERATION,-MODERATE, FOCAL 2866 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1262 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91277 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/07/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 222.9 GRAMS DATE AND TIME OF NECROPSY: 06/07/99 14:53 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-MINIMAL, MULTI-FOCAL SINGLE MASS, 50 X 45 X 25 MM, RED, -INFLAMMATION, ACUTE,-MODERATE, MULTI- DARK RED, TAN, FIRM; SAME ON CUT FOCAL SURFACE; COLLECTED; REGIONAL LN -NECROSIS,-SEVERE, MULTI-FOCAL (INGUINAL) NOT IDENTIFIED -M-CARCINOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY MATERIAL -ANIMAL THIN -------------------------------------------- 2867 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1263 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91277 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/07/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 222.9 GRAMS DATE AND TIME OF NECROPSY: 06/07/99 14:53 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2868 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1264 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91278 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 420.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:38 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK OVARY (OV) : OVARY (OV) : 105; 10 X 10MM; VERIFIED AT NECROPSY: -CYST(S); LEFT: SINGLE CLEAR FLUID >UNREMARKABLE NOT OBSERVED FILLED CYST, 6 MM IN DIAMETER >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK PITUITARY (PI) : PITUITARY (PI) : 105; 20 X 20MM; VERIFIED AT NECROPSY: -RED FOCUS(I)/AREA(S); RIGHT AND LEFT -HEMORRHAGE,-MODERATE, MULTI-FOCAL OBSERVED ASPECT: SINGLE DARK RED AREA ON EACH, -B-ADENOMA,-NEOPLASM PRESENT EACH IS 2 X 1 MM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT VENTRAL CERVICAL -M-CARCINOMA,- 2 NEOPLASMS PRESENT REGION: SINGLE MASS, 25 X 18 X 15 MM, TAN, RED, DARK RED AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE MANDIBULAR) -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 22 X 14 X 13 MM, TAN, RED, DARK RED AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY) CERVIX (CV) : CERVIX (CV) : -MASS(ES); ATTACHED TO RIGHT SEROSAL -HYPERTROPHY, SMOOTH MUSCLE,-MODERATE, SURFACE, EXTENDING TOWARDS VAGINA: DIFFUSE SINGLE MASS, 20 X 6 X 5 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 24) 2869 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1265 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91278 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 420.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:38 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -DILATATION,-MODERATELY SEVERE, DIFFUSE MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 7 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; -HEMORRHAGE,-SLIGHT, MULTI-FOCAL COLLECTED LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2870 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1266 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91278 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 420.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:38 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), KIDNEY (KD), PANCREAS (PA), LUNG (LU), THYROID (TY), URINARY BLADDER (UB), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 2871 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1267 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91279 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 524.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:21 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 53; 70 X 70MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE OBSERVED LIVER (LI) : LIVER (LI) : >MASS(ES); INGUINAL REGION-LEFT; WEEK -RED FOCUS(I)/AREA(S); ALONG MARGIN OF -FOCUS, ALTERED HEPATOCELLULAR, 80; 20 X 20MM; VERIFIED AT NECROPSY: RIGHT MEDIAN LOBE: SINGLE DARK RED EOSINOPHILIC,-SLIGHT, MULTI-FOCAL NOT OBSERVED AREA, 4 MM IN DIAMETER; COLLECTED ON -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- >SKIN & PELAGE; SORE/SCAB; VENTRAL- ROUTINE SECTION FOCAL ABDOMINAL-LEFT; VERIFIED AT NECROPSY: KIDNEY (KD) : OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >MASS(ES); AXILLARY REGION-RIGHT; WEEK FOCAL 96; 35 X 30MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MODERATE, OBSERVED FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 8 X 5 X 5 MM (RIGHT IS 5 -DEGENERATION, CYSTIC, CORTICAL,- X 4 X 4 MM) MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -VACUOLATION,-MODERATELY SEVERE, FOCAL LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -LARGE; UP TO 18 MM IN DIAMETER -DILATATION, SINUSOIDAL,-MODERATELY -RED FOCUS(I)/AREA(S); MULTIPLE DARK SEVERE, MULTI-FOCAL RED AREAS, UP TO 10 MM IN DIAMETER -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT 2872 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1268 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91279 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 524.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:21 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 8 X 7 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL SINGLE MASS, 20 X 15 X 15 MM, DARK -PIGMENT,-MODERATE, FOCAL RED, TAN, LOBULATED AND FIRM; SAME ON -B-ADENOMA,-NEOPLASM PRESENT CUT SURFACE (CAP 20); REGIONAL LN -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT (AXILLARY) NORMAL (CAP 21) -M-CARCINOMA,- 2 NEOPLASMS PRESENT -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 40 X 30 X 18 MM, TAN, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 21) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #3; RIGHT INGUINAL REGION: SINGLE MASS, 15 X 12 X 10 MM, DARK RED, TAN AND SOFT; ON SECTION EXUDES A BROWN SEMIFLUID (CAP 23); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #4; LEFT INGUINAL REGION: SINGLE MASS, 80 X 65 X 35 MM, RED, DARK RED, TAN, GRAY, LOBULATED AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED 2873 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1269 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91279 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 524.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:21 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #5; LEFT PERINEAL REGION: SINGLE MASS, 25 X 18 X 14 MM, RED, DARK RED, GRAY, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE (CAP 24); OVERLYING SKIN HAS A SINGLE ULCERATED AREA, 2 MM IN DIAMETER; COLLECTED INTACT WITH MASS; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : UTERUS (UT) : -CYST(S); MID PORTION OF LEFT HORN, -DILATATION,-MODERATELY SEVERE, DIFFUSE WITHIN WALL: SINGLE CLEAR FLUID FILLED -B-POLYP, ENDOMETRIAL, STROMAL,- CYST, 6 MM IN DIAMETER NEOPLASM PRESENT -RED FOCUS(I)/AREA(S); PROXIMAL END OF LEFT HORN, MUCOSAL SURFACE: SINGLE DARK RED AREA, 4 X 3 MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -DILATATION, SINUSOIDAL,-MINIMAL, MULTI- FOCAL -EDEMA,-SLIGHT, MULTI-FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DARK GREEN AND MOIST -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2874 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1270 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91279 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 524.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:21 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LUNG (LU), SKIN (SK), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2875 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1271 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91280 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/07/99 STUDY DAY OF DEATH: 681 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 389.1 GRAMS DATE AND TIME OF NECROPSY: 12/07/99 10:51 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -RAISED FOCUS(I)/AREA(S); CAUDATE -VACUOLATION, HEPATOCELLULAR,-MINIMAL, NECROPSY: NOT APPLICABLE LOBE, NEAR HILUS: SINGLE DARK RED, TAN MULTI-FOCAL >APPEARANCE; HUNCHED; VERIFIED AT AND RAISED AREA, 8 X 5 X 5 MM (CAP 21) -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM NECROPSY: NOT APPLICABLE PRESENT >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT OBSERVED -MINERALIZATION, PELVIC,-MODERATE, >BEHAVIOR; ATAXIC; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 9 X 8 X 8 MM (RIGHT IS 5 -ANGIECTASIS,-SEVERE, FOCAL X 4 X 3 MM) -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 8 X 5 MM, DARK RED, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE BROWN AREAS, UP TO 1 MM IN DIAMETER 2876 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1272 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91280 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/07/99 STUDY DAY OF DEATH: 681 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 389.1 GRAMS DATE AND TIME OF NECROPSY: 12/07/99 10:51 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL UTERUS (UT) : -DILATATION,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -HEPATOCELLULAR ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY DARK RED MATERIAL -DISCHARGE-NASAL; DRY DARK RED MATERIAL -------------------------------------------- 2877 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1273 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91280 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/07/99 STUDY DAY OF DEATH: 681 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 389.1 GRAMS DATE AND TIME OF NECROPSY: 12/07/99 10:51 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2878 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1274 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91281 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 263.4 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:30 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0475 .0180 % .0238 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0385 .0146 % .0193 WEIGHT TAKEN BRAIN (BR) 1.9995 .7591 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.1798 .4479 % .5900 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.2833 .4872 % .6418 WEIGHT TAKEN LIVER (LI) 9.9915 3.7933 % 4.9970 WEIGHT TAKEN LUNG (LU) 1.2442 .4724 % .6223 WEIGHT TAKEN LEFT OVARY (OV0) .0784 .0298 % .0392 WEIGHT TAKEN RIGHT OVARY (OV1) .0779 .0296 % .0390 WEIGHT TAKEN SPLEEN (SP) .4918 .1867 % .2460 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0074 .0028 % .0037 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0094 .0036 % .0047 WEIGHT TAKEN UTERUS (UT) .5260 .1997 % .2631 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED >NOT REQUIRED TO BE EXAMINED FOR ANIMAL (CAP 20) 2879 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1275 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91281 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 263.4 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:30 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -URINARY BLADDER-SMALL LUMEN; 4 X 3 X 1 MM -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2880 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1276 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91282 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/25/99 STUDY DAY OF DEATH: 365 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 274.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/99 9:56 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; BROWN HAIR COAT; BRAIN (BR) : BRAIN (BR) : PERINEAL AREA; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >DISCHARGE; NASAL; RED IN COLOR; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HEMORRHAGE,-SLIGHT, MULTI-FOCAL >EYES; RED DISCHARGE; EYES; VERIFIED AT -NECROSIS, HEPATOCELLULAR,-SLIGHT, NECROPSY: OBSERVED MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -VACUOLATION, HEPATOCELLULAR,-SLIGHT, NECROPSY: NOT APPLICABLE MULTI-FOCAL >EXCRETION; FEW FECES; VERIFIED AT THYMUS (TH) : NECROPSY: NOT APPLICABLE -DEPLETION, LYMPHOCYTIC,-MODERATELY >EYES; SQUINTED; PERI-ORBITAL; VERIFIED SEVERE, DIFFUSE AT NECROPSY: NOT APPLICABLE PARATHYROID (PT) : >RESPIRATION; AUDIBLE; VERIFIED AT >SECTION EXAMINED; TISSUE NOT PRESENT NECROPSY: NOT APPLICABLE PITUITARY (PI) : PITUITARY (PI) : >RESPIRATION; LABORED; VERIFIED AT -MASS(ES); 13 X 8 X 8 MM, RED AND SOFT -B-ADENOMA,-NEOPLASM PRESENT NECROPSY: NOT APPLICABLE STOMACH, GL (ST) : STOMACH, GL (ST) : -MASS(ES); PYLORIC MUCOSA: TWO BROWN >UNREMARKABLE ERODED AREAS, EACH IS 2 MM IN DIAMETER CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DARK BROWN AND DRY -BLOOD FILM TAKEN 2881 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1277 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91282 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/25/99 STUDY DAY OF DEATH: 365 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 274.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/99 9:56 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -DISCHARGE-NASAL; DARK RED AND DRY -GI TRACT-DARK DRY CONTENT; CECUM: DARK GREEN, IMPACTED, FECAL-LIKE MATERIAL -------------------------------------------- 2882 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1278 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91282 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/25/99 STUDY DAY OF DEATH: 365 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 274.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/99 9:56 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2883 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1279 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91283 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 282.2 GRAMS DATE AND TIME OF NECROPSY: 10/03/99 13:56 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 82; 20 X 20MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; -HEMORRHAGE,-MINIMAL, FOCAL WEEK 82; 35 X 30MM; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >SKIN & PELAGE; YELLOW HAIR COAT; FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR,-MINIMAL, >SKIN & PELAGE; SORE/SCAB; PAW-HIND- MULTI-FOCAL RIGHT; VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : >APPEARANCE; THIN; VERIFIED AT -MINERALIZATION, PELVIC,-SLIGHT, FOCAL NECROPSY: OBSERVED ADRENAL, CORTEX (AC) : >SKIN & PELAGE; PALE; ENTIRE BODY; -DEGENERATION, CYSTIC, CORTICAL,- VERIFIED AT NECROPSY: NOT APPLICABLE MODERATE, FOCAL >BEHAVIOR; ATAXIC; VERIFIED AT -HEMORRHAGE,-SLIGHT, FOCAL NECROPSY: NOT APPLICABLE LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY DARK; LIGHT BROWN >UNREMARKABLE THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL GRAY AND SOFT -B-ADENOMA,-NEOPLASM PRESENT 2884 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1280 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91283 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 282.2 GRAMS DATE AND TIME OF NECROPSY: 10/03/99 13:56 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-MODERATELY SEVERE, MULTI- SINGLE MASS, 25 X 25 X 20 MM, TAN AND FOCAL FIRM; ON SECTION CONTAINS A TAN -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT CASEOUS SEMISOLID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 23) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 35 X 35 X 15 MM, TAN, YELLOW, LOBULATED AND SOFT; SOME SECTIONS CONTAIN A TAN CASEOUS SEMISOLID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MULTIPLE CLEAR AND RED FLUID FILLED MODERATE, FOCAL CYSTS, UP TO 6 MM IN DIAMETER CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: -FIBROSIS,-MODERATE, FOCAL SINGLE TAN AND RAISED AREA, 10 X 6 X 6 -INFLAMMATION, ACUTE,-MINIMAL, MULTI- MM; COLLECTED FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW AND DRY -BLOOD FILM TAKEN -DISCHARGE-NASAL; DARK RED AND DRY -ANIMAL THIN -------------------------------------------- 2885 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1281 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91283 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 282.2 GRAMS DATE AND TIME OF NECROPSY: 10/03/99 13:56 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2886 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1282 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91284 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/20/99 9:52 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID >UNREMARKABLE FILLED CYST, 7 MM IN DIAMETER PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 5 X 4 MM -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -LIPOSARCOMA,-PRESENT CAVITY, THORACIC (TA) : CAVITY, THORACIC (TA) : -MASS(ES); ATTACHED TO DIAPHRAGM AND -M-LIPOSARCOMA,-NEOPLASM PRESENT THYMUS: SINGLE MASS, 25 X 20 X 20 MM, TAN, DARK RED, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MEDIASTINAL) NORMAL (CAP 21) 2887 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1283 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91284 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/20/99 9:52 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -FLUID-THORACIC CAVITY; CONTAINS A LARGE AMOUNT OF DARK RED FLUID -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2888 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1284 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91285 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 202.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:06 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 6.5999 3.2560 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2889 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1285 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91285 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 202.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:06 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2890 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1286 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91286 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 381.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:27 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT ANTERIOR -HEMORRHAGE,-MODERATELY SEVERE, FOCAL ASPECT: SINGLE DARK RED, SLIGHTLY -B-ADENOMA,-NEOPLASM PRESENT RAISED AREA, 3 X 3 X 2 MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2891 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1287 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91286 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 381.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:27 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), LUNG (LU), MAMMARY, FEMALE (MF), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2892 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1288 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91287 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 168.4 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:52 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 5.8456 3.4713 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2893 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1289 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91287 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 168.4 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:52 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2894 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1290 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91288 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 261.9 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:26 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0295 .0113 % .0157 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0332 .0127 % .0176 WEIGHT TAKEN BRAIN (BR) 1.8834 .7191 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.2385 .4729 % .6576 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.2662 .4835 % .6723 WEIGHT TAKEN LIVER (LI) 8.8606 3.3832 % 4.7046 WEIGHT TAKEN LUNG (LU) 1.1983 .4575 % .6362 WEIGHT TAKEN LEFT OVARY (OV0) .0327 .0125 % .0174 WEIGHT TAKEN RIGHT OVARY (OV1) .0481 .0184 % .0255 WEIGHT TAKEN SPLEEN (SP) .4713 .1800 % .2502 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0092 .0035 % .0049 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0089 .0034 % .0047 WEIGHT TAKEN UTERUS (UT) .8430 .3219 % .4476 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LN, MESENTERIC (MS) : VERIFIED AT NECROPSY: OBSERVED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: SINGLE >NOT REQUIRED TO BE EXAMINED FOR ANIMAL PINPOINT AREA UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CLEAR FLUID CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2895 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1291 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91288 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 261.9 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:26 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2896 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1292 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91289 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 224.2 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 13:55 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; HEAD TILT; LEFT; VERIFIED -DILATATION, VENTRICULAR,-PRESENT AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >EXCRETION; FEW FECES; VERIFIED AT -MINERALIZATION, PELVIC,-SLIGHT, MULTI- NECROPSY: NOT APPLICABLE FOCAL >APPEARANCE; THIN; VERIFIED AT SPLEEN (SP) : NECROPSY: NOT OBSERVED -PIGMENT, INCREASED,-MINIMAL, MULTI- >RESPIRATION; AUDIBLE; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE ADRENAL, CORTEX (AC) : >RESPIRATION; IRREGULAR; VERIFIED AT -ANGIECTASIS,-SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE -DEGENERATION, CYSTIC, CORTICAL,- >BEHAVIOR; ATAXIC; VERIFIED AT MODERATE, FOCAL NECROPSY: NOT APPLICABLE -HEMORRHAGE,-SLIGHT, MULTI-FOCAL >APPEARANCE; HUNCHED; VERIFIED AT OVARY (OV) : NECROPSY: NOT APPLICABLE -CYST,-PRESENT >DISCHARGE; ORAL; CLEAR; VERIFIED AT THYMUS (TH) : NECROPSY: OBSERVED -CYST,-PRESENT >SKIN & PELAGE; COLD TO TOUCH; LIMBS- LUNG (LU) : ALL; VERIFIED AT NECROPSY: NOT -INFILTRATE, MACROPHAGE, ALVEOLAR,- APPLICABLE SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 13 X 10 X 10 -HEMORRHAGE,-SLIGHT, MULTI-FOCAL MM, RED, DARK RED, BROWN AND FIRM -B-ADENOMA,-NEOPLASM PRESENT 2897 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1293 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91289 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 224.2 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 13:55 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL RECTUM (RE) : -PARASITISM,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-ORAL; CLEAR AND MOIST -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: UPPER INCISORS -------------------------------------------- 2898 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1294 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91289 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 224.2 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 13:55 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2899 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1295 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91290 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/08/99 STUDY DAY OF DEATH: 407 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: 294.0 GRAMS DATE AND TIME OF NECROPSY: 03/08/99 13:28 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); AXILLARY REGION-RIGHT; WEEK -DILATATION, VENTRICULAR,-PRESENT 52; 10 X 10MM; VERIFIED AT NECROPSY: LIVER (LI) : OBSERVED -TELANGIECTASIS,-SLIGHT, FOCAL >APPEARANCE; HEAD TILT; RIGHT; VERIFIED KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >APPEARANCE; THIN; VERIFIED AT FOCAL NECROPSY: NOT OBSERVED -MINERALIZATION, TUBULAR,-SLIGHT, MULTI- FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 8 X 5 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- TAN AND SOFT FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL STOMACH, NONGL (SU) : -EDEMA,-SLIGHT, FOCAL 2900 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1296 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91290 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/08/99 STUDY DAY OF DEATH: 407 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: 294.0 GRAMS DATE AND TIME OF NECROPSY: 03/08/99 13:28 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 24 X 14 X 10 MM, RED, -PIGMENT,-SLIGHT, MULTI-FOCAL DARK RED, TAN, FIRM, AND LOBULATED; -M-CARCINOMA,-NEOPLASM PRESENT SOME SECTIONS EXUDE A DARK RED FLUID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED -MALOCCLUSION; BILATERAL: UPPER INCISORS -------------------------------------------- 2901 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1297 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91290 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/08/99 STUDY DAY OF DEATH: 407 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: 294.0 GRAMS DATE AND TIME OF NECROPSY: 03/08/99 13:28 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2902 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1298 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91291 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 207.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:11 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.1834 3.4569 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2903 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1299 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91291 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 207.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:11 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2904 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1300 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91292 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 741.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:57 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -HYPERPLASIA, BILE DUCT,-SLIGHT, MULTI- FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 3 MM IN DIAMETER THYROID (TY) : THYROID (TY) : -LARGE; BILATERAL: LEFT IS 7 X 5 X 5 -B-ADENOMA, C-CELL,- 2 NEOPLASMS MM, RIGHT IS 7 X 4 X 3 MM PRESENT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 5 X 5 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -GALACTOCELE,-MINIMAL, MULTI-FOCAL AND FIRM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 2905 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1301 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91292 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 741.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:57 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, UPPER INCISORS -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2906 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1302 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91293 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 278.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:25 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0309 .0111 % .0152 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0277 .0099 % .0136 WEIGHT TAKEN BRAIN (BR) 2.0350 .7299 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.0502 .3767 % .5161 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.0631 .3813 % .5224 WEIGHT TAKEN LIVER (LI) 8.1243 2.9140 % 3.9923 WEIGHT TAKEN LUNG (LU) 1.1760 .4218 % .5779 WEIGHT TAKEN LEFT OVARY (OV0) .0804 .0288 % .0395 WEIGHT TAKEN RIGHT OVARY (OV1) .0675 .0242 % .0332 WEIGHT TAKEN SPLEEN (SP) .4346 .1559 % .2136 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0134 .0048 % .0066 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0187 .0067 % .0092 WEIGHT TAKEN UTERUS (UT) .8268 .2966 % .4063 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2907 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1303 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91293 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 278.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:25 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2908 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1304 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91294 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 455.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:12 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: >UNREMARKABLE MULTIPLE DARK RED AREAS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 5 MM IN DIAMETER (RIGHT -DEGENERATION, CYSTIC, CORTICAL,- IS 4 X 3 X 2 MM) MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATELY SEVERE, MULTI- FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, GRAY, AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -GALACTOCELE,-MODERATE, FOCAL AND FIRM; COLLECTED ON ROUTINE SECTION -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -HEMORRHAGE,-MODERATELY SEVERE, FOCAL -M-CARCINOMA,-NEOPLASM PRESENT 2909 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1305 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91294 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 455.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:12 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : -MASS(ES); WITHIN WALL OF RIGHT HORN: SINGLE MASS, 10 MM IN DIAMETER, DARK RED, TAN AND FIRM; ON SECTION EXUDES A DARK RED SEMIFLUID; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 20) -SEMISOLID MATERIAL; WITHIN LUMEN OF RIGHT HORN: DARK RED SEMISOLID VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE DARK RED MATERIAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: DARK RED AND TAN; -HEMORRHAGE,-SLIGHT, MULTI-FOCAL COLLECTED CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -MASS(ES); FREE FLOATING WITHIN -NECROSIS, FAT,-PRESENT ABDOMINAL CAVITY: SINGLE MASS, 4 X 3 X 2 MM, YELLOW AND FIRM; COLLECTED; REGIONAL LN (RENAL) NOT IDENTIFIED LN, LUMBAR/ILIAC (LM) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: RED -BONE MARROW SMEAR TAKEN -------------------------------------------- 2910 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1306 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91294 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 455.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:12 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), LN, MEDIASTINAL (ML), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LUNG (LU) ------------------------------------------------------------------------------------------------------------------------------------ 2911 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1307 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91295 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 454.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:00 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 87; 30 X 30MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK LIVER (LI) : LIVER (LI) : 99; 50 X 55MM; VERIFIED AT NECROPSY: -LIGHT FOCUS(I)/AREA(S); LEFT MEDIAN -VACUOLATION, HEPATOCELLULAR,-SLIGHT, OBSERVED LOBE: SINGLE TAN AREA, 5 X 3 MM (CAP MULTI-FOCAL 20) -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM -RED FOCUS(I)/AREA(S); ALL LOBES: PRESENT MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 8 X 7 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN, GRAY AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -GALACTOCELE,-MODERATELY SEVERE, MULTI- SINGLE MASS, 60 X 35 X 20 MM, RED, FOCAL DARK RED, TAN, LOBULATED, AND SOFT; -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT SOME SECTIONS EXUDE A TAN CREAMY SEMIFLUID (CAP 21) REGIONAL LN (AXILLARY) NORMAL (CAP 22) 2912 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1308 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91295 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 454.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:00 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 63 X 50 X 25 MM, RED, DARK RED, TAN, VASCULAR AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-MINIMAL, FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2913 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1309 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91295 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 454.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:00 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2914 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1310 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91296 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 217.3 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:25 PROSECTOR: DENNIS HOFFMAN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: OBSERVED -ANGIECTASIS,-SLIGHT, MULTI-FOCAL >SKIN & PELAGE; YELLOW HAIR COAT; -HEMORRHAGE,-SLIGHT, MULTI-FOCAL VERIFIED AT NECROPSY: OBSERVED PITUITARY (PI) : PITUITARY (PI) : >APPEARANCE; HUNCHED; VERIFIED AT -MASS(ES); SINGLE MASS, 6 X 6 X 12 MM, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE FIRM, RED, DARK RED AND TAN -B-ADENOMA,-NEOPLASM PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT MAMMARY, FEMALE (MF) : NECROPSY: NOT APPLICABLE -GALACTOCELE,-SLIGHT, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 2915 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1311 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91296 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 217.3 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:25 PROSECTOR: DENNIS HOFFMAN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2916 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1312 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91297 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 636 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/23/99 12:23 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 90; 50 X 40; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -NECROSIS, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -DIFFUSELY RED; RIGHT: DARK RED -DEGENERATION, CYSTIC, CORTICAL,- -SMALL; LEFT: 3 X 2 X 2 MM (RIGHT IS 4 SEVERE, FOCAL X 3 X 3 MM) -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 5 MM, RED, DARK -HEMORRHAGE,-SLIGHT, MULTI-FOCAL RED, TAN, AND SOFT -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2917 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1313 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91297 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 636 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/23/99 12:23 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL SKIN (SK) : -M-FIBROSARCOMA,-NEOPLASM PRESENT CERVIX (CV) : CERVIX (CV) : -LARGE; 10 MM IN DIAMETER >UNREMARKABLE -FIRM; DIFFUSE CAUSE OF DEATH (CD) : -FIBROSARCOMA,-PRESENT SUBCUTANEOUS TIS (SQ) : SUBCUTANEOUS TIS (SQ) : -MASS(ES); RIGHT DORSAL LUMBAR REGION, >NOT REQUIRED TO BE EXAMINED FOR ANIMAL EXTENDING TO RIGHT LATERAL ABDOMINAL >NOTE:>FIBROSARCOMA ENTERED UNDER SKIN REGION: SINGLE MASS, 70 X 60 X 40 MM, RED, DARK RED, TAN, SOFT, GELATINOUS, AND FRIABLE; SAME ON CUT SURFACE; ON SECTION, SOME AREAS EXUDED A DARK RED, CLOTTED SEMIFLUID; ANIMAL IDENTIFICATION TRANSPONDER FOUND WITHIN MASS; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -------------------------------------------- 2918 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1314 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91297 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 636 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/23/99 12:23 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2919 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1315 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91298 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 328.7 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 13:39 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >MASS(ES); AXILLARY REGION-LEFT; WEEK FOCAL 43; 55 X 55MM; VERIFIED AT NECROPSY: ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : OBSERVED -LARGE; LEFT: 7 X 6 X 5 MM (FOR -ANGIECTASIS,-MODERATE, MULTI-FOCAL >APPEARANCE; THIN; VERIFIED AT COMPARISON, RIGHT IS 5 X 5 X 3 MM) -HEMORRHAGE,-SLIGHT, MULTI-FOCAL NECROPSY: OBSERVED -CYST(S); LEFT, DIFFUSE: MULTIPLE RED >SKIN & PELAGE; YELLOW HAIR COAT; FLUID FILLED CYSTS, 3 MM IN DIAMETER VERIFIED AT NECROPSY: OBSERVED OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: MULTIPLE CLEAR -CYST,-PRESENT FLUID FILLED CYSTS ON EACH, UP TO 2 MM IN DIAMETER THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -FOREIGN BODY,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, FOCAL -INFLAMMATION, GRANULOMATOUS,-SLIGHT, MULTI-FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, MULTI-FOCAL SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT 2920 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1316 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91298 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 328.7 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 13:39 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -MINERALIZATION,-MINIMAL, MULTI-FOCAL SINGLE MASS, 81 X 55 X 24 MM, TAN, >NOTE:>MAMMARY MASS DESCRIBED GROSSLY RED, LOBULATED, VASCULAR AND SOFT; WAS A SUBCUTANEOUS FIBROMA SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 20) UTERUS (UT) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL CAUSE OF DEATH (CD) : -FIBROMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS 6 X 3 X 2 >UNREMARKABLE MM; COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: MOIST YELLOW MATERIAL -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: LOWER INCISORS -ANIMAL THIN -------------------------------------------- 2921 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1317 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91298 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 328.7 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 13:39 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2922 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1318 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91299 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 216.1 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:52 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0346 .0160 % .0185 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0309 .0143 % .0165 WEIGHT TAKEN BRAIN (BR) 1.8673 .8641 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) .9484 .4389 % .5079 WEIGHT TAKEN RIGHT KIDNEY (KD1) .9537 .4413 % .5107 WEIGHT TAKEN LIVER (LI) 7.0761 3.2745 % 3.7895 WEIGHT TAKEN LUNG (LU) 1.0849 .5020 % .5810 WEIGHT TAKEN LEFT OVARY (OV0) .0498 .0230 % .0267 WEIGHT TAKEN RIGHT OVARY (OV1) .0500 .0231 % .0268 WEIGHT TAKEN SPLEEN (SP) .3660 .1694 % .1960 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0070 .0032 % .0037 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0094 .0043 % .0050 WEIGHT TAKEN UTERUS (UT) .4825 .2233 % .2584 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2923 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1319 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91299 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 216.1 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:52 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2924 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1320 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91300 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/27/99 9:48 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: OBSERVED -ANGIECTASIS,-MINIMAL, FOCAL >SKIN & PELAGE; YELLOW HAIR COAT; -HEMORRHAGE,-MINIMAL, FOCAL VERIFIED AT NECROPSY: NOT OBSERVED LN, MESENTERIC (MS) : >EXCRETION; FEW FECES; VERIFIED AT -INFILTRATE, MACROPHAGE, PIGMENTED,- NECROPSY: NOT APPLICABLE MINIMAL, MULTI-FOCAL >RESPIRATION; AUDIBLE; VERIFIED AT LUNG (LU) : LUNG (LU) : NECROPSY: NOT APPLICABLE -DIFFUSELY RED; ALL LOBES: DARK RED >UNREMARKABLE -UNCOLLAPSED; ALL LOBES PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- RED, TAN AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT VENTRAL THORACIC -GALACTOCELE,-MINIMAL, MULTI-FOCAL REGION: SINGLE MASS, 10 X 10 X 5 MM, -B-ADENOMA,-NEOPLASM PRESENT BROWN, TAN AND SOFT; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (AXILLARY) NORMAL (CAP 21) -THICKENED; ALL REGIONS, DIFFUSE: TAN; COLLECTED ON ROUTINE SECTION 2925 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1321 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91300 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/27/99 9:48 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY DARK BROWN MATERIAL -ANIMAL THIN -------------------------------------------- 2926 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1322 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91300 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/27/99 9:48 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2927 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1323 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91301 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 671.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:39 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 99; 30 X 30MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED KIDNEY (KD) : >MASS(ES); INGUINAL REGION-LEFT; WEEK -MINERALIZATION, PELVIC,-SLIGHT, MULTI- 105; 20 X 20MM; VERIFIED AT NECROPSY: FOCAL NOT OBSERVED LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); LEFT ANTERIOR -CYST,-PRESENT ASPECT: SINGLE DARK BROWN FOCUS, 1 MM -B-ADENOMA,-NEOPLASM PRESENT IN DIAMETER -LARGE; 6 X 6 X 5 MM; APPEARS SWOLLEN MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL SINGLE MASS, 60 X 40 X 30 MM, RED, -B-FIBROADENOMA,-NEOPLASM PRESENT DARK RED, TAN, VASCULAR, LOBULATED AND -M-CARCINOMA,-NEOPLASM PRESENT FIRM; SOME PORTIONS EXUDE A DARK RED SEMIFLUID MATERIAL; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) 2928 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1324 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91301 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 671.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:39 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #2; LEFT PERINEAL REGION: SINGLE MASS, 40 X 30 X 15 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (LUMBAR) NORMAL (CAP 21); POSSIBLY CONSISTENT WITH THE MASS (INGUINAL- LEFT) NOTED IN THE CLINICAL OBSERVATIONS -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2929 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1325 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91301 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 671.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:39 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB), LN, LUMBAR/ILIAC (LM), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2930 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1326 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91302 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/13/99 STUDY DAY OF DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 218.6 GRAMS DATE AND TIME OF NECROPSY: 06/13/99 14:05 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; YELLOW HAIR COAT; -DILATATION, VENTRICULAR,-PRESENT VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : KIDNEY (KD) : >SKIN & PELAGE; ROUGH HAIR COAT; -LARGE PELVIS(ES); LEFT -DILATATION, PELVIC,-PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MINIMAL, MULTI- >APPEARANCE; HUNCHED; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE SPLEEN (SP) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -PIGMENT, INCREASED,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >SKIN & PELAGE; COLD TO TOUCH; ENTIRE ADRENAL, CORTEX (AC) : BODY; VERIFIED AT NECROPSY: NOT -DEGENERATION, CYSTIC, CORTICAL,- APPLICABLE SEVERE, FOCAL >EXCRETION; FEW FECES; VERIFIED AT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 15 X 10 X 9 -HEMORRHAGE,-MODERATELY SEVERE, MULTI- MM, RED, DARK RED, TAN AND FIRM FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -B-FIBROADENOMA,-NEOPLASM PRESENT AND FIRM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2931 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1327 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91302 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/13/99 STUDY DAY OF DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 218.6 GRAMS DATE AND TIME OF NECROPSY: 06/13/99 14:05 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MEDIASTINAL (ML) : -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW MOIST MATERIAL -BLOOD FILM TAKEN -ANIMAL THIN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY -------------------------------------------- 2932 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1328 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91302 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/13/99 STUDY DAY OF DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 218.6 GRAMS DATE AND TIME OF NECROPSY: 06/13/99 14:05 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2933 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1329 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91303 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 589.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:53 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 100; 10 X 10MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NOT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); AXILLARY REGION-RIGHT; WEEK LIVER (LI) : LIVER (LI) : 105; VERIFIED AT NECROPSY: OBSERVED -MOTTLED; ALL LOBES: RED AND TAN -FOCUS, ALTERED HEPATOCELLULAR, CLEAR -RED FOCUS(I)/AREA(S); ALL LOBES: CELL,-MODERATE, FOCAL MULTIPLE DARK RED AREAS, UP TO 5 X 3 -FOCUS, ALTERED HEPATOCELLULAR, MM; COLLECTED ON ROUTINE SECTIONS EOSINOPHILIC,-SEVERE, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, DARK RED AND -HEMORRHAGE,-MODERATE, MULTI-FOCAL SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -HYPERPLASIA, GLANDULAR,-MODERATE, MULTI-FOCAL 2934 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1330 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91303 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 589.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:53 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -B-ADENOMA,-NEOPLASM PRESENT SINGLE MASS, 15 MM IN DIAMETER, TAN -M-CARCINOMA,-NEOPLASM PRESENT AND FIRM; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (AXILLARY) NORMAL (CAP 21) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 25 X 20 X 15 MM, RED, DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; REGIONAL LN (INGUINAL) NORMAL (CAP 22) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM (CAP 23) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -GI TRACT-DARK FLUID CONTENT; STOMACH, DUODENUM AND JEJUNUM: DARK BROWN SEMIFLUID MATERIAL -BONE MARROW SMEAR TAKEN -------------------------------------------- 2935 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1331 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91303 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 589.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:53 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB), LN, AXILLARY (AX), LN, INGUINAL (IN) ------------------------------------------------------------------------------------------------------------------------------------ 2936 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1332 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91304 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 300.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:23 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0421 .0140 % .0197 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0424 .0141 % .0198 WEIGHT TAKEN BRAIN (BR) 2.1378 .7107 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.2554 .4174 % .5872 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.2507 .4158 % .5850 WEIGHT TAKEN LIVER (LI) 10.4193 3.4639 % 4.8738 WEIGHT TAKEN LUNG (LU) 1.2114 .4027 % .5667 WEIGHT TAKEN LEFT OVARY (OV0) .0627 .0208 % .0293 WEIGHT TAKEN RIGHT OVARY (OV1) .0679 .0226 % .0318 WEIGHT TAKEN SPLEEN (SP) .4924 .1637 % .2303 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0094 .0031 % .0044 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0130 .0043 % .0061 WEIGHT TAKEN UTERUS (UT) .6986 .2322 % .3268 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; VENTRAL- LIVER (LI) : CERVICAL-LEFT; VERIFIED AT NECROPSY: -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NOT OBSERVED FOCAL >SKIN & PELAGE; SORE/SCAB; VENTRAL- SKIN (SK) : SKIN (SK) : CERVICAL-RIGHT; VERIFIED AT NECROPSY: -ALOPECIA-FOCAL; CERVICAL REGION: TWO >NOT REQUIRED TO BE EXAMINED FOR ANIMAL NOT OBSERVED AREAS, ONE IS 25 X 21 MM AND THE OTHER IS 15 X 10 MM (CAP 20) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 2937 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1333 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91304 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 300.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:23 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2938 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1334 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91305 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 557.0 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 10:24 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; PARALYSIS; LIMBS-HIND; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- SLIGHT, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 4 MM IN DIAMETER THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 4 X 5 X 9 MM, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL FIRM, LOBULATED, RED, DARK RED AND TAN -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT 2939 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1335 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91305 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 557.0 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 10:24 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 12 X 14 X 21 MM, FIRM, RED, TAN AND LOBULATED; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL -BLOOD FILM TAKEN -------------------------------------------- 2940 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1336 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91305 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 557.0 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 10:24 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2941 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1337 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91306 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 455.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:17 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: AS ASSIGNED ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 74; 60 X 50MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE OBSERVED LIVER (LI) : >MASS(ES); AXILLARY REGION-RIGHT; WEEK -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- 85; 10 X 10MM; VERIFIED AT NECROPSY: FOCAL OBSERVED KIDNEY (KD) : >MASS(ES); INGUINAL REGION-RIGHT; WEEK -CAST, PROTEINACEOUS,-MINIMAL, FOCAL 92; 35 X 25MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MINIMAL, FOCAL OBSERVED ADRENAL, CORTEX (AC) : >MASS(ES); INGUINAL REGION-LEFT; WEEK -ANGIECTASIS,-MINIMAL, MULTI-FOCAL 93; 20 X 20MM; VERIFIED AT NECROPSY: -HEMORRHAGE,-MINIMAL, MULTI-FOCAL OBSERVED -B-ADENOMA,-NEOPLASM PRESENT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID >UNREMARKABLE FILLED CYST, 5 MM IN DIAMETER LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, BROWN, TAN -HEMORRHAGE,-SLIGHT, MULTI-FOCAL AND SOFT -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -EDEMA,-MODERATE, MULTI-FOCAL 2942 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1338 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91306 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 455.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:17 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: AS ASSIGNED ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : -INFLAMMATION, CHRONIC,-MODERATE, MULTI- FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-MODERATELY SEVERE, MULTI- SINGLE MASS, 17 X 15 X 15 MM, DARK FOCAL RED, BROWN, TAN AND SOFT; SAME ON CUT -HEMORRHAGE,-MODERATELY SEVERE, MULTI- SURFACE; (CAP 21); REGIONAL LN (SEE FOCAL AXILLARY) -INFLAMMATION, CHRONIC,-MODERATE, MULTI- -MASS #2; LEFT AXILLARY REGION: SINGLE FOCAL MASS, 90 X 70 X 40 MM, DARK RED, TAN, -NECROSIS,-MODERATELY SEVERE, MULTI- LOBULATED AND FIRM; SAME ON CUT FOCAL SURFACE; COLLECTED; REGIONAL LN (SEE -B-FIBROADENOMA,-NEOPLASM PRESENT AXILLARY); OVERLYING SKIN HAS A SINGLE -M-CARCINOMA,- 3 NEOPLASMS PRESENT DARK RED CRUSTED AREA, 40 X 30 MM, COLLECTED INTACT -MASS #3; RIGHT INGUINAL REGION: SINGLE MASS, 20 X 17 X 17 MM, DARK RED, BROWN AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 26) -MASS #4; LEFT INGUINAL REGION: SINGLE MASS, 45 X 40 X 30 MM, TAN, BROWN, AND FIRM; CUT SURFACE CONTAINS A BROWN FLUID; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 26) UTERUS (UT) : UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE >UNREMARKABLE 2943 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1339 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91306 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 455.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:17 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: AS ASSIGNED ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 15 X 10 X 5 MM -INFILTRATE, MACROPHAGE, PIGMENTED,- (RIGHT IS UP TO 9 X 5 X 3 MM) (CAP 22) MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2944 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1340 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91306 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 455.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:17 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: AS ASSIGNED ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, INGUINAL (IN) ------------------------------------------------------------------------------------------------------------------------------------ 2945 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1341 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91307 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 394.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:15 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY RED; DARK RED >UNREMARKABLE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 7 X 6 MM, RED, DARK -HEMORRHAGE,-SLIGHT, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2946 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1342 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91307 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 394.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:15 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2947 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1343 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91308 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/20/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 319.3 GRAMS DATE AND TIME OF NECROPSY: 05/20/99 8:51 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HEAD TILT; RIGHT; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >BEHAVIOR; ATAXIC; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE -HEMORRHAGE,-MODERATE, FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -MOTTLED; GREEN AND TAN -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -CYST,-PRESENT 2948 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1344 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91308 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/20/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 319.3 GRAMS DATE AND TIME OF NECROPSY: 05/20/99 8:51 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 17 X 10 X 8 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- GRAY, AND SOFT FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2949 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1345 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91308 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/20/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 319.3 GRAMS DATE AND TIME OF NECROPSY: 05/20/99 8:51 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2950 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1346 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91309 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/04/99 STUDY DAY OF DEATH: 464 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 466.4 GRAMS DATE AND TIME OF NECROPSY: 05/04/99 14:35 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK KIDNEY (KD) : 57; 40 X 45MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MODERATE, OBSERVED MULTI-FOCAL >MASS(ES); VENTRAL-CERVICAL; WEEK 62; -MINERALIZATION, TUBULAR,-MINIMAL, 30 X 30MM; VERIFIED AT NECROPSY: MULTI-FOCAL OBSERVED SPLEEN (SP) : SPLEEN (SP) : >SKIN & PELAGE; SORE/SCAB; VENTRAL- -LARGE; 60 X 15 X 10 MM -HEMATOPOIESIS, EXTRAMEDULLARY, ABDOMINAL-LEFT; VERIFIED AT NECROPSY: INCREASED,-MODERATE, MULTI-FOCAL OBSERVED ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- SLIGHT, FOCAL OVARY (OV) : -CYST,-PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); LEFT POSTERIOR -HEMORRHAGE,-SLIGHT, MULTI-FOCAL ASPECT: SINGLE DARK GREY FOCUS, 1 MM -B-ADENOMA,-NEOPLASM PRESENT IN DIAMETER -LARGE; 5 X 5 X 3 MM, APPEARS SWOLLEN MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 2951 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1347 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91309 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/04/99 STUDY DAY OF DEATH: 464 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 466.4 GRAMS DATE AND TIME OF NECROPSY: 05/04/99 14:35 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -INFLAMMATION, ACUTE,-MODERATE, MULTI- SINGLE MASS, 45 X 40 X 25 MM, RED, FOCAL DARK RED, TAN, SOFT AND LOBULATED; ON -NECROSIS,-SLIGHT, MULTI-FOCAL SECTION EXUDED A BROWN AND TAN CASEOUS -PIGMENT,-SLIGHT, MULTI-FOCAL SEMISOLID MATERIAL (COLLECTED); -B-FIBROADENOMA,-NEOPLASM PRESENT OVERLYING SKIN HAS MULTIPLE ULCERATED -M-CARCINOMA,-NEOPLASM PRESENT AREAS, UP TO 20 MM IN DIAMETER (COLLECTED INTACT WITH MASS); REGIONAL LN (INGUINAL) NORMAL (CAP 20) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 15 MM IN DIAMETER (CAP 21) -MASS #2; LEFT SHOULDER REGION: SINGLE MASS, 50 X 20 X 15 MM, RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE (COLLECTED); REGIONAL LN (SEE AXILLARY) UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -DILATATION,-MODERATELY SEVERE, DIFFUSE MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 8 X 5 X 5 MM CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 15 X 8 X 3 MM (FOR -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, COMPARISON, RIGHT IS UP TO 5 X 3 X 1 MULTI-FOCAL MM) (CAP 22) 2952 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1348 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91309 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/04/99 STUDY DAY OF DEATH: 464 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 466.4 GRAMS DATE AND TIME OF NECROPSY: 05/04/99 14:35 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, INGUINAL (IN) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2953 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1349 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91310 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 500.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 9:43 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK LIVER (LI) : 71; 80 X 95MM; VERIFIED AT NECROPSY: -HEMORRHAGE,-MINIMAL, MULTI-FOCAL OBSERVED -NECROSIS, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 6 X 3 MM, APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 100 X 75 X 30 MM, RED, -HYPERPLASIA, GLANDULAR,-MODERATE, DARK RED, TAN, LOBULATED, AND MULTI-FOCAL VASCULAR; SAME ON CUT SURFACE; PORTION -NECROSIS,-MODERATE, MULTI-FOCAL COLLECTED; REGIONAL LN (AXILLARY) -B-FIBROADENOMA,-NEOPLASM PRESENT NORMAL (CAP 20) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : >TISSUE MISSING 2954 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1350 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91310 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 500.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 9:43 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2955 Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Author: Peter J. Thomford, PhD Study Completion Date: December 13, 2001 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-212 Sponsor Study Identification: 3M T-6316.1 Volume VIII of XII Page 2956 of 4873 Covance 6329-212 3M T-6316.1 VOLUME VIII OF XII CONTENTS Page APPENDIX 6 (Continued) ........................................................................................ 2958 Individual Anatomic Pathology Data ..................................................................... 2959 APPENDIX 7............................................................................................................ 3299 Cell Proliferation Report ....................................................................................... 3300 APPENDIX 8............................................................................................................ 3338 Electron Microscopy Report .................................................................................. 3339 2957 APPENDIX 6 (Continued) Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 2958 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1351 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91311 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/14/99 STUDY DAY OF DEATH: 444 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/14/99 10:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC -NECROSIS, HEPATOCELLULAR,-MODERATE, SURFACE OF LEFT LATERAL LOBE: SINGLE MULTI-FOCAL TAN AREA, 3 X 2 MM; COLLECTED ON ROUTINE SECTION -MOTTLED; ALL LOBES: DARK BROWN AND TAN PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR -CYST,-PRESENT FLUID FILLED CYST ON EACH, LEFT IS 9 MM IN DIAMETER, RIGHT IS 4 MM IN DIAMETER PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); MID ASPECT: -B-ADENOMA,-NEOPLASM PRESENT SINGLE DARK BROWN FOCUS, 1 MM IN DIAMETER STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK BROWN, PINPOINT FOCI UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: -DILATATION,-SEVERE, DIFFUSE DARK RED FLUID -HEMORRHAGE,-MODERATELY SEVERE, DIFFUSE 2959 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1352 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91311 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/14/99 STUDY DAY OF DEATH: 444 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/14/99 10:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : -LARGE, DIFFUSE; BILATERAL: EACH IS UP TO 15 MM IN DIAMETER CAUSE OF DEATH (CD) : -UTERINE HEMORRHAGE,-PRESENT -------------------------------------------- 2960 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1353 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91311 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/14/99 STUDY DAY OF DEATH: 444 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/14/99 10:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2961 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1354 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91312 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 675 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 200.1 GRAMS DATE AND TIME OF NECROPSY: 12/01/99 13:47 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >DISCHARGE; GENITAL; RED IN COLOR; -DILATATION, VENTRICULAR,-PRESENT VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 6 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, GREY AND SOFT -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL 2962 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1355 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91312 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 675 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 200.1 GRAMS DATE AND TIME OF NECROPSY: 12/01/99 13:47 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SALIV GL, MANDIB (SG) : >TISSUE MISSING STOMACH, GL (ST) : -EDEMA,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- CLEAR FLUID MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW AND RED -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED -ANIMAL THIN -------------------------------------------- 2963 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1356 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91312 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 675 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 200.1 GRAMS DATE AND TIME OF NECROPSY: 12/01/99 13:47 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2964 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1357 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91313 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/25/99 STUDY DAY OF DEATH: 608 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 357.8 GRAMS DATE AND TIME OF NECROPSY: 09/25/99 8:40 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 71; 40 X 40MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED KIDNEY (KD) : >MASS(ES); AXILLARY REGION-LEFT; WEEK -CAST, PROTEINACEOUS,-SLIGHT, MULTI- 74; VERIFIED AT NECROPSY: OBSERVED FOCAL >SKIN & PELAGE; RED HAIR COAT; VENTRAL- -MINERALIZATION, PELVIC,-SLIGHT, MULTI- ABDOMINAL; VERIFIED AT NECROPSY: NOT FOCAL OBSERVED ADRENAL, CORTEX (AC) : >SKIN & PELAGE; RED HAIR COAT; VENTRAL- -ANGIECTASIS,-MINIMAL, MULTI-FOCAL THORACIC; VERIFIED AT NECROPSY: NOT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL OBSERVED LN, MESENTERIC (MS) : >MASS(ES); AXILLARY REGION-RIGHT; WEEK -DILATATION, SINUSOIDAL,-SLIGHT, MULTI- 85; 25 X 30MM; VERIFIED AT NECROPSY: FOCAL OBSERVED THYMUS (TH) : >MASS(ES); VENTRAL-ABDOMINAL; WEEK 85; -M-THYMIC CARCINOMA,-NEOPLASM PRESENT 45 X 45MM; VERIFIED AT NECROPSY: PITUITARY (PI) : PITUITARY (PI) : OBSERVED -MASS(ES); SINGLE MASS, 7 X 8 X 13 MM, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL >APPEARANCE; HEAD TILT; RIGHT; VERIFIED FIRM, RED, DARK RED AND TAN -PIGMENT,-MINIMAL, MULTI-FOCAL AT NECROPSY: NOT APPLICABLE -B-ADENOMA,-NEOPLASM PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : NECROPSY: NOT APPLICABLE -MASS(ES); LEFT AXILLARY REGION: -HEMORRHAGE,-MODERATE, MULTI-FOCAL SINGLE MASS, 6 X 9 X 13 MM, FIRM, RED, -B-FIBROADENOMA,- 4 NEOPLASMS PRESENT TAN AND LOBULATED; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (AXILLARY) NORMAL (CAP 22) 2965 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1358 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91313 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/25/99 STUDY DAY OF DEATH: 608 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 357.8 GRAMS DATE AND TIME OF NECROPSY: 09/25/99 8:40 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 13 X 15 X 30 MM, FIRM, RED, DARK BROWN, TAN AND LOBULATED; ON SECTION EXUDES A DARK BROWN SEMIFLUID (CAP 23); REGIONAL LN (AXILLARY) NORMAL (CAP 22) -MASS #3; LEFT INGUINAL REGION: SINGLE MASS, 20 X 36 X 45 MM, FIRM, RED, DARK RED, TAN, BROWN AND LOBULATED; ON SECTION EXUDES A BROWN FLUID (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #4; LEFT PERIANAL REGION: SINGLE MASS, 25 X 45 X 55 MM, FIRM, RED, DARK RED, TAN AND LOBULATED; SAME ON CUT SURFACE (CAP 25); REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -THYMIC CARCINOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2966 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1359 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91313 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/25/99 STUDY DAY OF DEATH: 608 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 357.8 GRAMS DATE AND TIME OF NECROPSY: 09/25/99 8:40 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2967 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1360 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91314 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 314.2 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 13:33 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 61; 35 X 35MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >APPEARANCE; THIN; VERIFIED AT FOCAL NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, MULTI- >SKIN & PELAGE; RED HAIR COAT; VENTRAL- FOCAL ABDOMINAL; VERIFIED AT NECROPSY: ADRENAL, CORTEX (AC) : OBSERVED -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -VACUOLATION,-MINIMAL, FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR -CYST,-PRESENT FLUID FILLED CYST ON EACH, EACH IS 6 MM IN DIAMETER LUNG (LU) : -THROMBUS,-MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 13 X 7 X 6 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, DARK RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT 2968 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1361 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91314 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 314.2 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 13:33 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT CERVICAL REGION, -GALACTOCELE,-MODERATE, MULTI-FOCAL EXTENDS TO RIGHT AXILLARY REGION: -HEMORRHAGE,-MODERATE, MULTI-FOCAL SINGLE MASS, 58 X 37 X 26 MM, RED, -PIGMENT,-MINIMAL, MULTI-FOCAL DARK RED, TAN, BROWN, FIRM AND -M-CARCINOMA,-NEOPLASM PRESENT LOBULATED; ON SECTION, SOME AREAS EXUDE A DARK BROWN FLUID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 20) CERVIX (CV) : CERVIX (CV) : -LARGE; 8 X 6 X 5 MM >UNREMARKABLE CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: UPPER INCISORS -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 2969 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1362 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91314 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 314.2 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 13:33 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2970 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1363 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91315 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 477.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:01 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 99; 10 X 10MM; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); LEFT LATERAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- >MASS(ES); DORSAL; WEEK 103; 50 X 40MM AND RIGHT MEDIAN LOBES: MULTIPLE TAN FOCAL SIDE RIGHT; VERIFIED AT NECROPSY: AREAS, UP TO 6 MM IN DIAMETER; -VACUOLATION, HEPATOCELLULAR,-MINIMAL, OBSERVED COLLECTED ON ROUTINE SECTIONS MULTI-FOCAL -MASS(ES); PAPILLARY PROCESS: SINGLE -B-ADENOMA, HEPATOCELLULAR,- 2 MASS, 15 X 12 X 8 MM, RED, DARK RED, NEOPLASMS PRESENT TAN AND SOFT; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (HEPATIC) NOT IDENTIFIED -RED FOCUS(I)/AREA(S); LEFT LATERAL LOBE: MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS -MASS #2; RIGHT LATERAL LOBE: SINGLE MASS, 15 X 15 X 7 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (HEPATIC) NOT IDENTIFIED KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, FOCAL -HEMORRHAGE,-MODERATE, FOCAL 2971 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1364 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91315 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 477.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:01 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -THROMBUS,-MODERATE, FOCAL THYMUS (TH) : -CYST,-PRESENT SKIN (SK) : SKIN (SK) : -MASS(ES); DORSAL LUMBAR REGION: -FIBROSIS,-MODERATELY SEVERE, FOCAL SINGLE MASS, 15 X 15 X 8 MM, TAN AND -INFLAMMATION, ACUTE,-MODERATELY FIRM; SAME ON CUT SURFACE (CAP 23); SEVERE, FOCAL ANIMAL IDENTIFICATION TRANSPONDER -ULCERATION,-SEVERE, FOCAL FOUND WITHIN MASS; REGIONAL LN (SEE INGUINAL) MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 70 X 60 X 30 MM, RED, -B-FIBROADENOMA,-NEOPLASM PRESENT DARK RED, TAN, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE INGUINAL) CERVIX (CV) : CERVIX (CV) : -LIGHT FOCUS(I)/AREA(S); TRIMMING -DILATATION,-MODERATE, FOCAL COMMENT: WITHIN LUMEN: SINGLE TAN -B-LEIOMYOMA,-NEOPLASM PRESENT AREA, 3 X 3 X 2 MM; TRIMMED ON ROUTINE SECTION UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MODERATE, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 2972 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1365 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91315 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 477.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:01 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; COLLECTED LN, INGUINAL (IN) : LN, INGUINAL (IN) : -DIFFUSELY DARK; LEFT: BROWN (RIGHT IS -HEMORRHAGE,-MINIMAL, MULTI-FOCAL NORMAL) (CAP 22) -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT, MULTI-FOCAL PINNA (PN) : PINNA (PN) : -RED FOCUS(I)/AREA(S); BILATERAL, -HEMORRHAGE,-SLIGHT, FOCAL DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 5 X 2 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2973 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1366 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91315 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 477.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:01 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2974 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1367 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91316 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 193.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:30 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 6.6687 3.4499 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2975 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1368 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91316 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 193.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:30 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2976 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1369 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91317 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 212.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:57 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.3592 3.4599 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2977 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1370 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91317 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 212.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:57 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 2978 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1371 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91318 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (A) DATE OF DEATH: 05/07/98 STUDY DAY OF DEATH: 102 STUDY WEEK OF DEATH: 15 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/07/98 14:41 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -LACERATION; DIFFUSE: MULTIPLE -HEMORRHAGE,-MODERATE, MULTI-FOCAL LACERATIONS; TISSUE IS DARK RED, GELATINOUS AND AMORPHIC KIDNEY (KD) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL LUNG (LU) : LUNG (LU) : -MOTTLED; ALL LOBES: RED AND DARK RED -HEMORRHAGE,-MODERATE, MULTI-FOCAL -UNCOLLAPSED; ALL LOBES PITUITARY (PI) : PITUITARY (PI) : -MISSING/NOT IDENTIFIED; NOT >TISSUE MISSING IDENTIFIED DUE TO DIFFUSE FRACTURES OF CALVARIUM HARDERIAN GLAND (HG) : HARDERIAN GLAND (HG) : >NOTE:>TRIM COMMENT: NOT FOUND AT TIME >TISSUE MISSING OF TRIM CAUSE OF DEATH (CD) : -FRACTURE, CALVARIUM,-PRESENT CALVARIUM/SKULL (CA) : CALVARIUM/SKULL (CA) : -FRACTURE(S); DIFFUSE: MULTIPLE -FRACTURE,-PRESENT FRACTURES; COLLECTED INTACT -HEMORRHAGE,-MODERATE, MULTI-FOCAL GENERAL COMMENT (GC) : -DISCHARGE-ORAL; DARK RED FLUID -DISCHARGE-NASAL; DARK RED FLUID 2979 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1372 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91318 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (A) DATE OF DEATH: 05/07/98 STUDY DAY OF DEATH: 102 STUDY WEEK OF DEATH: 15 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/07/98 14:41 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -STAINS - SKIN/HAIRCOAT; HEAD: DARK RED, MOIST MATERIAL -TRACHEA-CONTENT FOAMY MATTER; DARK RED, FOAMY MATERIAL -------------------------------------------- 2980 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1373 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91318 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (A) DATE OF DEATH: 05/07/98 STUDY DAY OF DEATH: 102 STUDY WEEK OF DEATH: 15 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/07/98 14:41 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA) ------------------------------------------------------------------------------------------------------------------------------------ 2981 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1374 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91319 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/26/99 STUDY DAY OF DEATH: 486 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 274.1 GRAMS DATE AND TIME OF NECROPSY: 05/26/99 14:11 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >DISCHARGE; ORAL; CLEAR; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: OBSERVED KIDNEY (KD) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -CAST, PROTEINACEOUS,-SLIGHT, FOCAL NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, MULTI- >RESPIRATION; IRREGULAR; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 3 MM IN DIAMETER PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 9 X 8 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, GREY, LOBULATED AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-ORAL; MOIST, CLEAR MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL 2982 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1375 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91319 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/26/99 STUDY DAY OF DEATH: 486 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 274.1 GRAMS DATE AND TIME OF NECROPSY: 05/26/99 14:11 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2983 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1376 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91320 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/26/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 314.7 GRAMS DATE AND TIME OF NECROPSY: 07/26/99 13:30 PROSECTOR: JILL PAUS RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; RED HAIR COAT; HEAD- -DILATATION, VENTRICULAR,-PRESENT ENTIRE; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; YELLOW HAIR COAT; -LIGHT FOCUS(I)/AREA(S); RIGHT MEDIAN -VACUOLATION, HEPATOCELLULAR,-SLIGHT, VERIFIED AT NECROPSY: OBSERVED LOBE: SINGLE TAN AREA, 9 X 2 MM; MULTI-FOCAL >BEHAVIOR; ATAXIC; VERIFIED AT COLLECTED ON ROUTINE SECTION NECROPSY: NOT APPLICABLE -MOTTLED; ALL LOBES, DIFFUSE: BROWN AND TAN KIDNEY (KD) : KIDNEY (KD) : -DIFFUSELY DARK; BILATERAL: DARK BROWN -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LIGHT FOCUS(I)/AREA(S); BILATERAL: >UNREMARKABLE MULTIPLE TAN, PINPOINT FOCI -DIFFUSELY DARK; BILATERAL: BROWN PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 8 X 6 X 5 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, DARK RED, TAN, SOFT AND LOBULATED -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM 2984 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1377 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91320 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/26/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 314.7 GRAMS DATE AND TIME OF NECROPSY: 07/26/99 13:30 PROSECTOR: JILL PAUS RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -------------------------------------------- 2985 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1378 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91320 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/26/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 314.7 GRAMS DATE AND TIME OF NECROPSY: 07/26/99 13:30 PROSECTOR: JILL PAUS RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2986 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1379 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91321 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/10/99 STUDY DAY OF DEATH: 654 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 406.7 GRAMS DATE AND TIME OF NECROPSY: 11/10/99 14:59 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 90; 20 X 20MM; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; SORE/SCAB; LUMBAR-LEFT; -DILATATION, VENTRICULAR,-PRESENT VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : >EYES; PALE; EYES; VERIFIED AT -MINERALIZATION, PELVIC,-MODERATE, NECROPSY: NOT APPLICABLE MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, FOCAL -FIBROSIS,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 10 X 7 -HEMORRHAGE,-MODERATE, MULTI-FOCAL MM, RED, DARK RED, GREY, AND SOFT -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI- FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL 2987 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1380 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91321 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/10/99 STUDY DAY OF DEATH: 654 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 406.7 GRAMS DATE AND TIME OF NECROPSY: 11/10/99 14:59 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 22 X 20 X 10 MM, TAN, -B-FIBROADENOMA,-NEOPLASM PRESENT RED, AND SOFT; SAME ON CUT SURFACE; -M-CARCINOMA,-NEOPLASM PRESENT COLLECTED; OVERLYING SKIN HAS A SINGLE TAN ULCERATED AREA; COLLECTED INTACT WITH MASS; REGIONAL LN (INGUINAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH MASS (DORSAL) NOTED IN CLINICAL OBSERVATIONS UTERUS (UT) : -DILATATION,-SLIGHT, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2988 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1381 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91321 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/10/99 STUDY DAY OF DEATH: 654 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 406.7 GRAMS DATE AND TIME OF NECROPSY: 11/10/99 14:59 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2989 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1382 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91322 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/25/98 STUDY DAY OF DEATH: 151 STUDY WEEK OF DEATH: 22 TERMINAL BODY WEIGHT: 275.1 GRAMS DATE AND TIME OF NECROPSY: 06/25/98 9:20 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT OVARY (OV) : NECROPSY: OBSERVED -MINERALIZATION,-MINIMAL, FOCAL >SKIN & PELAGE; BROWN HAIR COAT; CAUSE OF DEATH (CD) : VENTRAL-ABDOMINAL; VERIFIED AT -UNDETERMINED,-PRESENT NECROPSY: OBSERVED GENERAL COMMENT (GC) : >DISCHARGE; NASAL; RED IN COLOR; -STAINS-PERINEUM/PERIANAL; YELLOWISH- VERIFIED AT NECROPSY: OBSERVED BROWN >APPEARANCE; THIN; VERIFIED AT -BLOOD FILM TAKEN NECROPSY: NOT APPLICABLE -DISCHARGE-OCULAR; BILATERAL: DARK RED >EXCRETION; NO FECES; VERIFIED AT AND DRY MATERIAL NECROPSY: NOT APPLICABLE -DISCHARGE-NASAL; BILATERAL: DARK RED >RESPIRATION; AUDIBLE; VERIFIED AT AND DRY MATERIAL NECROPSY: NOT APPLICABLE -MALOCCLUSION; BILATERAL, UPPER INCISORS -------------------------------------------- 2990 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1383 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91322 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/25/98 STUDY DAY OF DEATH: 151 STUDY WEEK OF DEATH: 22 TERMINAL BODY WEIGHT: 275.1 GRAMS DATE AND TIME OF NECROPSY: 06/25/98 9:20 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2991 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1384 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91323 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 343.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:55 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0385 .0112 % .0166 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0328 .0096 % .0141 WEIGHT TAKEN BRAIN (BR) 2.3182 .6759 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.3772 .4015 % .5941 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.3727 .4002 % .5921 WEIGHT TAKEN LIVER (LI) 19.1752 5.5904 % 8.2716 WEIGHT TAKEN LUNG (LU) 1.7596 .5130 % .7590 WEIGHT TAKEN LEFT OVARY (OV0) .0546 .0159 % .0236 WEIGHT TAKEN RIGHT OVARY (OV1) .0493 .0144 % .0213 WEIGHT TAKEN SPLEEN (SP) .4801 .1400 % .2071 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0143 .0042 % .0062 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0102 .0030 % .0044 WEIGHT TAKEN UTERUS (UT) 1.1650 .3397 % .5025 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; ROUGH HAIR COAT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; LIVER (LI) : WEEK 51; 10 X 10MM; VERIFIED AT -HYPERTROPHY, CENTRILOBULAR, NECROPSY: OBSERVED HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL OVARY (OV) : -HEMORRHAGE,-MINIMAL, FOCAL 2992 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1385 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91323 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 343.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:55 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -FIBROSIS,-MODERATE, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EROSION,-SLIGHT, FOCAL MULTIPLE DARK BROWN AREAS, UP TO 3 X 1 MM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 25 X 17 X 10 MM, RED, DARK RED, TAN, LOBULATED, CYSTIC AND FIRM; SOME SECTIONS EXUDE A DARK RED FLUID; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN AND CREAMY SEMIFLUID FILLED CYSTS; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION 2993 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1386 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91323 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 343.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:55 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2994 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1387 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91324 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 316.0 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:10 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LIVER (LI) : LIVER (LI) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -DIFFUSELY DARK; ALL LOBES: DARK BROWN -TELANGIECTASIS,-SLIGHT, FOCAL -DARK FOCUS(I)/AREA(S); ALL LOBES: -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTIPLE DARK BROWN AREAS, UP TO 2 MM MULTI-FOCAL IN DIAMETER; COLLECTED ON ROUTINE -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM SECTIONS PRESENT LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL THYMUS (TH) : THYMUS (TH) : -CYST(S); AT BIFURCATION OF LOBES: -CYST,-PRESENT SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER; COLLECTED THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT, FOCAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 5 X 4 X 3 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL 2995 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1388 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91324 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 316.0 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:10 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : UTERUS (UT) : -THICKENED WALL; BILATERAL: DIFFUSE >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: -EROSION,-MODERATE, FOCAL SINGLE RED, DRY, CRUSTED AREA, 3 X 2 -INFLAMMATION, CHRONIC ACTIVE,-SLIGHT, MM; COLLECTED FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2996 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1389 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91324 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 316.0 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:10 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2997 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1390 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91325 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 320.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:06 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; HEAD- BRAIN (BR) : BRAIN (BR) : CRANIAL; VERIFIED AT NECROPSY: NOT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -MOTTLED; ALL LOBES: DARK BROWN AND -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >BEHAVIOR; HYPOACTIVE; VERIFIED AT TAN FOCAL NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR,-SLIGHT, >SKIN & PELAGE; BROWN HAIR COAT; MULTI-FOCAL PERINEAL AREA; VERIFIED AT NECROPSY: KIDNEY (KD) : KIDNEY (KD) : OBSERVED -DIFFUSELY DARK; BILATERAL: DARK BROWN -MINERALIZATION, PELVIC,-MINIMAL, MULTI- >EYES; RED DISCHARGE; EYES; VERIFIED AT FOCAL NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >APPEARANCE; HUNCHED; VERIFIED AT MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 8 X 8 X 7 MM (LEFT IS 5 -ANGIECTASIS,-SLIGHT, MULTI-FOCAL X 4 X 4 MM) -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -VACUOLATION,-MODERATELY SEVERE, FOCAL -B-ADENOMA,-NEOPLASM PRESENT LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, MACROPHAGE, ALVEOLAR,- MULTIPLE TAN AREAS, UP TO 5 MM IN SLIGHT, MULTI-FOCAL DIAMETER THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 2998 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1391 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91325 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 320.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:06 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -LARGE; RIGHT: 7 X 5 X 5 MM (LEFT IS 5 X 4 X 4 MM) PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 11 X 7 MM; DARK RED, -CYST,-PRESENT TAN AND SOFT -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE GREEN CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW AND DRY -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -------------------------------------------- 2999 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1392 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91325 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 320.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:06 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3000 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1393 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91326 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 212.7 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:48 PROSECTOR: TERRI L. WILLIAMS RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -DIFFUSELY DARK; ALL LOBES: DARK BROWN -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >SKIN & PELAGE; YELLOW HAIR COAT; FOCAL VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : >SKIN & PELAGE; RED HAIR COAT; LIMBS- -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FRONT; VERIFIED AT NECROPSY: NOT FOCAL OBSERVED THYMUS (TH) : >APPEARANCE; HUNCHED; VERIFIED AT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NECROPSY: NOT APPLICABLE NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT THYROID (TY) : NECROPSY: NOT APPLICABLE -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 7 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -GALACTOCELE,-MINIMAL, MULTI-FOCAL AND FIRM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT CAVITY, ABDOM (PC) : -NECROSIS, FAT,-PRESENT 3001 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1394 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91326 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 212.7 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:48 PROSECTOR: TERRI L. WILLIAMS RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE NEAR RIGHT OVARY: SINGLE MASS, 8 X 3 X 2 MM, YELLOW AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT WITH OVARY; REGIONAL LN (ANTERIOR MESENTERIC) NOT IDENTIFIED HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DARK YELLOW -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED -DISCHARGE-NASAL; RED -ANIMAL THIN -------------------------------------------- 3002 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1395 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91326 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 212.7 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:48 PROSECTOR: TERRI L. WILLIAMS RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN) ------------------------------------------------------------------------------------------------------------------------------------ 3003 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1396 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91327 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 493.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:45 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 84; 30 X 30MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ON MARGIN OF -FOCUS, ALTERED HEPATOCELLULAR, LEFT MEDIAN LOBE: SINGLE RED AREA, 8 X EOSINOPHILIC,-MODERATELY SEVERE, FOCAL 7 MM; COLLECTED ON ROUTINE SECTION -TELANGIECTASIS,-MODERATE, FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI- FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -HYPERPLASIA, CORTICAL,-MODERATELY SEVERE, MULTI-FOCAL -MINERALIZATION,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, FOCAL -THROMBUS,-MODERATE, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 6 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- TAN AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT 3004 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1397 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91327 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 493.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:45 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 40 X 25 X 15 MM, RED, -M-CARCINOMA,-NEOPLASM PRESENT DARK RED, TAN, LOBULATED AND FIRM; SOME SECTIONS EXUDE A DARK BROWN FLUID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) -THICKENED; ALL REGIONS, DIFFUSE: COLLECTED ON ROUTINE SECTION UTERUS (UT) : UTERUS (UT) : -CYST(S); RIGHT HORN, WITHIN LUMEN: -DILATATION,-MODERATE, DIFFUSE SINGLE BROWN FLUID FILLED CYST, 10 X 3 X 3 MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3005 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1398 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91327 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 493.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:45 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 3006 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1399 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91328 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 437 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 336.1 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 13:55 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT ADRENAL, MEDULLA (MA) : NECROPSY: OBSERVED -ONE EXAMINED,-PRESENT >DISCHARGE; ORAL; RED IN COLOR; OVARY (OV) : OVARY (OV) : VERIFIED AT NECROPSY: OBSERVED -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT FILLED CYST, 2 MM IN DIAMETER NECROPSY: OBSERVED THYROID (TY) : >RESPIRATION; LABORED; VERIFIED AT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT NECROPSY: NOT APPLICABLE PITUITARY (PI) : PITUITARY (PI) : >RESPIRATION; AUDIBLE; VERIFIED AT -DARK FOCUS(I)/AREA(S); MID POSTERIOR -HEMORRHAGE,-MINIMAL, FOCAL NECROPSY: NOT APPLICABLE ASPECT: SINGLE DARK BROWN FOCUS, 2 MM -B-ADENOMA,-NEOPLASM PRESENT >SKIN & PELAGE; RED HAIR COAT; DORSAL- IN DIAMETER CERVICAL-RIGHT; VERIFIED AT NECROPSY: UTERUS (UT) : UTERUS (UT) : OBSERVED -LUMEN FILLED WITH FLUID; RIGHT: CLEAR >UNREMARKABLE >EYES; PALE; EYES; VERIFIED AT FLUID NECROPSY: NOT APPLICABLE CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN; -HEMORRHAGE,-SLIGHT, MULTI-FOCAL COLLECTED GENERAL COMMENT (GC) : -DISCHARGE-ORAL; DRY, RED MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -DISCHARGE-NASAL; DRY, RED MATERIAL 3007 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1400 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91328 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 437 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 336.1 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 13:55 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, UPPER INCISORS -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE AND HEAD REGION: DRY, RED MATERIAL -------------------------------------------- 3008 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1401 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91328 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 437 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 336.1 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 13:55 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3009 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1402 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91329 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 229.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:56 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0247 .0108 % .0121 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0268 .0117 % .0131 WEIGHT TAKEN BRAIN (BR) 2.0389 .8884 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) .9396 .4094 % .4608 WEIGHT TAKEN RIGHT KIDNEY (KD1) .9792 .4267 % .4803 WEIGHT TAKEN LIVER (LI) 9.5614 4.1662 % 4.6895 WEIGHT TAKEN LUNG (LU) 1.1670 .5085 % .5724 WEIGHT TAKEN LEFT OVARY (OV0) .0495 .0216 % .0243 WEIGHT TAKEN RIGHT OVARY (OV1) .0478 .0208 % .0234 WEIGHT TAKEN SPLEEN (SP) .3983 .1736 % .1954 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0098 .0043 % .0048 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0122 .0053 % .0060 WEIGHT TAKEN UTERUS (UT) .4650 .2026 % .2281 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, DIFFUSE ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN 3010 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1403 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91329 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 229.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:56 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL INFORMATION (XX) : >NOTE:>ANIMAL DIED FOLLOWING SAMPLE COLLECTION AND PRIOR TO ANESTHESIA; NO ANESTHESIA ADMINISTERED -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3011 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1404 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91330 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 406.5 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 14:41 PROSECTOR: JERALD W. JOHNSON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-THORACIC; WEEK 54; BRAIN (BR) : BRAIN (BR) : 50 X 45MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >MASS(ES); DORSAL; WEEK 68; 35 X 35MM; -FOCUS, ALTERED HEPATOCELLULAR, CLEAR VERIFIED AT NECROPSY: OBSERVED CELL,-MODERATE, FOCAL >MASS(ES); DORSAL-CERVICAL-LEFT; WEEK KIDNEY (KD) : 80; 30 X 30MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-SLIGHT, MULTI- OBSERVED FOCAL >SKIN & PELAGE; SORE/SCAB; SACRAL-LEFT; SPLEEN (SP) : SPLEEN (SP) : VERIFIED AT NECROPSY: OBSERVED -LARGE; 57 X 14 X 7 MM -HEMATOPOIESIS, EXTRAMEDULLARY, >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK INCREASED,-MODERATE, DIFFUSE 84; 15 X 15MM ANTERIOR; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: OBSERVED -VACUOLATION,-SLIGHT, FOCAL >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; OVARY (OV) : OVARY (OV) : WEEK 85; 20 X 20 MM; VERIFIED AT -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT NECROPSY: OBSERVED FILLED CYST, 3 MM IN DIAMETER >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK PITUITARY (PI) : PITUITARY (PI) : 85; 20 X 20MM POSTERIOR; VERIFIED AT -MASS(ES); 8 X 7 X 5 MM, RED, TAN AND -B-ADENOMA,-NEOPLASM PRESENT NECROPSY: OBSERVED FIRM MAMMARY, FEMALE (MF) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI- FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL -MINERALIZATION,-MODERATE, FOCAL 3012 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1405 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91330 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 406.5 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 14:41 PROSECTOR: JERALD W. JOHNSON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 20 X 15 X 14 MM, DARK -M-CARCINOMA,- 5 NEOPLASMS PRESENT RED, GRAY, LOBULATED AND SOFT; SAME ON CUT SURFACE (CAP 24); REGIONAL LN (SEE INGUINAL); POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL LEFT POSTERIOR) AS NOTED IN CLINICAL OBSERVATIONS -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 30 X 25 X 15 MM, DARK RED, RED, TAN, GRAY AND FIRM; SAME ON CUT SURFACE; ON SECTION EXUDES A BROWN FLUID (CAP 25); REGIONAL LN (SEE AXILLARY); POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL LEFT) AS NOTED IN CLINICAL OBSERVATIONS -MASS #3; MID VENTRAL CERVICAL REGION: SINGLE MASS, 50 X 35 X 12 MM, TAN, GRAY, LOBULATED AND FIRM; SAME ON CUT SURFACE (CAP 26); REGIONAL LN (MANDIBULAR) NORMAL (CAP 22); POSSIBLY CONSISTENT WITH MASS (VENTRAL THORACIC) AS NOTED IN CLINICAL OBSERVATIONS 3013 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1406 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91330 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 406.5 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 14:41 PROSECTOR: JERALD W. JOHNSON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #4; LEFT LATERAL CERVICAL REGION: SINGLE MASS, 35 X 30 X 20 MM, DARK BROWN, BROWN, RED, LOBULATED AND SOFT; SAME ON CUT SURFACE (CAP 27); REGIONAL LN (MANDIBULAR) NORMAL (CAP 22); POSSIBLY CONSISTENT WITH MASS (DORSAL CERVICAL LEFT) AS NOTED IN CLINICAL OBSERVATIONS -MASS #5; DORSAL CERVICAL REGION: SINGLE MASS, 30 X 20 X 20 MM, TAN, RED, DARK RED, LOBULATED AND FIRM; SAME ON CUT SURFACE; ON SECTION EXUDES A BROWN SEMIFLUID MATERIAL (CAP 28); REGIONAL LN NORMAL (CAP 22); POSSIBLY CONSISTENT WITH MASS (DORSAL) AS NOTED IN CLINICAL OBSERVATIONS -MASS #6; PERIANAL REGION: TWO MASSES, EACH IS 20 X 15 X 10, BOTH ARE TAN, DARK RED, LOBULATED AND FIRM; BOTH ARE SAME ON CUT SURFACE (CAP 29); REGIONAL LN (SEE INGUINAL); POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL RIGHT) AS NOTED IN CLINICAL OBSERVATIONS; OVERLYING SKIN IS DARK BROWN, MOIST AND ULCERATED, 12 MM IN DIAMETER; COLLECTED INTACT WITH MASS 3014 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1407 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91330 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 406.5 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 14:41 PROSECTOR: JERALD W. JOHNSON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN LUMEN: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MULTIPLE CLEAR FLUID FILLED CYSTS, UP MINIMAL, MULTI-FOCAL TO 3 MM IN DIAMETER -THICKENED WALL; BILATERAL, DIFFUSE CAUSE OF DEATH (CD) : -PITUITARY CARCINOMA,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MULTI-FOCAL LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 7 X 5 X 2 MM -DILATATION, SINUSOIDAL,-SLIGHT, MULTI- (RIGHT IS UP TO 3 X 2 X 1 MM) (CAP 23) FOCAL -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, MULTI-FOCAL LN, INGUINAL (IN) : LN, INGUINAL (IN) : -LARGE; BILATERAL: EACH IS UP TO 4 X 4 -N-MAMMARY CARCINOMA,-NEOPLASM PRESENT MM (CAP 21) -DIFFUSELY DARK; LEFT: DARK BROWN (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3015 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1408 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91330 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 406.5 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 14:41 PROSECTOR: JERALD W. JOHNSON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3016 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1409 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91331 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/20/99 STUDY DAY OF DEATH: 541 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 256.8 GRAMS DATE AND TIME OF NECROPSY: 07/20/99 13:58 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; VENTRAL- BRAIN (BR) : BRAIN (BR) : ABDOMINAL; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; YELLOW HAIR COAT; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- >EXCRETION; FEW FECES; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >APPEARANCE; THIN; VERIFIED AT FOCAL NECROPSY: OBSERVED -NECROSIS, HEPATOCELLULAR,-MINIMAL, >EYES; SQUINTED; PERI-ORBITAL; VERIFIED MULTI-FOCAL AT NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -PIGMENT,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LIGHT FOCUS(I)/AREA(S); LEFT CORTEX: -DEGENERATION, CYSTIC, CORTICAL,- SINGLE TAN AREA, 2 MM IN DIAMETER MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT THYMUS (TH) : -CYST,-PRESENT 3017 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1410 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91331 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/20/99 STUDY DAY OF DEATH: 541 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 256.8 GRAMS DATE AND TIME OF NECROPSY: 07/20/99 13:58 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 9 X 5 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, GRAY, TAN, CYSTIC, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: -DILATATION,-MINIMAL, FOCAL CLEAR FLUID -B-POLYP, ENDOMETRIAL, STROMAL,- NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; PERINEUM: YELLOW AND MOIST -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL REGION: RED AND MOIST -ANIMAL THIN -------------------------------------------- 3018 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1411 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91331 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/20/99 STUDY DAY OF DEATH: 541 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 256.8 GRAMS DATE AND TIME OF NECROPSY: 07/20/99 13:58 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3019 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1412 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91332 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 306.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:16 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; DORSAL; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT OBSERVED LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- TAN, GREY AND SOFT FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- SLIGHT, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3020 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1413 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91332 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 306.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:16 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, ANT MES/PANC (AP) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL >NOTE:>PANCREATIC LYMPH NODE WAS PRESENT ON PANCREAS SLIDE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3021 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1414 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91332 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 306.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:16 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3022 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1415 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91333 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 187.0 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:30 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.5152 4.0188 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3023 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1416 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91333 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 187.0 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:30 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 3024 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1417 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91334 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/31/99 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/31/99 8:40 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT EYE (EY) : NECROPSY: OBSERVED -INFLAMMATION, ACUTE, CORNEAL,-MINIMAL, >MASS(ES); AXILLARY REGION-LEFT; WEEK MULTI-FOCAL 86; 30 X 30MM; VERIFIED AT NECROPSY: -ULCERATION, CORNEAL,-MODERATE, FOCAL OBSERVED -VASCULARIZATION, CORNEAL,-MODERATE, >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; FOCAL WEEK 97; 60 X 60MM; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -MINERALIZATION, TUBULAR,-MODERATELY >BEHAVIOR; HYPOACTIVE; VERIFIED AT SEVERE, MULTI-FOCAL NECROPSY: NOT APPLICABLE ADRENAL, CORTEX (AC) : >EXCRETION; FEW FECES; VERIFIED AT -ANGIECTASIS,-SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -MASS(ES); INVOLVING ENTIRE LEFT -ONE EXAMINED,-PRESENT OVARY: SINGLE MASS, 25 X 14 X 14 MM, >NOTE:>OVARIAN MASS DESCRIBED GROSSLY DARK RED, TAN AND FIRM; SAME ON CUT WAS NECROTIC MESENTERIC FAT SURFACE; COLLECTED; REGIONAL LN (RENAL) NORMAL (CAP 22) -MASS #2; ATTACHED TO MASS #1: SINGLE MASS, 40 X 30 X 25 MM, DARK GREEN, BROWN, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (RENAL) NORMAL (CAP 22) 3025 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1418 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91334 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/31/99 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/31/99 8:40 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -MINERALIZATION,-MINIMAL, FOCAL HEART (HT) : HEART (HT) : -DIFFUSELY LIGHT; TAN -DEGENERATION/NECROSIS,-MODERATELY SEVERE, MULTI-FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL -MINERALIZATION,-MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -MINERALIZATION,-MODERATE, MULTI-FOCAL MULTIPLE BLACK AND BROWN AREAS, UP TO 3 X 1 MM MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI- FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL -PIGMENT,-MODERATE, MULTI-FOCAL -B-ADENOMA,- 2 NEOPLASMS PRESENT 3026 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1419 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91334 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/31/99 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/31/99 8:40 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 35 X 20 X 10 MM, RED, DARK RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; OVERLYING SKIN HAS A SINGLE DARK RED AND DRY CRUSTED AREA, 7 X 4 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (AXILLARY) NORMAL (CAP 21) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 65 X 55 X 25 MM, RED, DARK RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -RENAL MINERALIZATION,-PRESENT CAVITY, ABDOM (PC) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI- FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, FOCAL -MINERALIZATION,-SLIGHT, MULTI-FOCAL -NECROSIS, FAT,-PRESENT -PIGMENT,-MINIMAL, MULTI-FOCAL -THROMBUS,-MODERATELY SEVERE, MULTI- FOCAL LN, RENAL (RL) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 3027 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1420 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91334 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/31/99 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/31/99 8:40 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, RENAL (RL) : -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT, MULTI-FOCAL LN, AXILLARY (AX) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -MALOCCLUSION; BILATERAL, UPPER INCISORS -ANIMAL THIN -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN OFF AT BASE -------------------------------------------- 3028 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1421 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91334 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/31/99 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/31/99 8:40 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3029 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1422 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91335 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 395.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:52 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -HYPERTROPHY, CENTRILOBULAR, MULTIPLE DARK RED AND SLIGHTLY HEPATOCELLULAR,-MINIMAL, DIFFUSE DEPRESSED AREAS, UP TO 4 MM IN -VACUOLATION, HEPATOCELLULAR,-MINIMAL, DIAMETER; COLLECTED ON ROUTINE MULTI-FOCAL SECTIONS LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 5 X 4 X 3 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE TAN AREAS, PINPOINT AND UP TO 1 MM IN DIAMETER 3030 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1423 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91335 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 395.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:52 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MINIMAL, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -------------------------------------------- 3031 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1424 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91335 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 395.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:52 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3032 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1425 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91336 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 335.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:10 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; BRAIN (BR) : BRAIN (BR) : WEEK 88; 10 X 15MM; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED -DILATATION, VENTRICULAR,-PRESENT LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MODERATE, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -THROMBUS,-MINIMAL, FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY RED; DARK RED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 3 MM IN DIAMETER 3033 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1426 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91336 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 335.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:10 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -CYST,-PRESENT THYROID (TY) : THYROID (TY) : -MISSING/NOT IDENTIFIED; LEFT: MISSING -CYST, FOLLICULAR,-MODERATE, FOCAL -ONE EXAMINED,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); MID PECTORAL REGION: SINGLE -B-ADENOMA,-NEOPLASM PRESENT MASS, 22 X 17 X 10 MM, RED, DARK RED, -M-CARCINOMA,- 2 NEOPLASMS PRESENT TAN AND SOFT; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (SEE AXILLARY) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 25 X 20 X 10 MM, RED, DARK RED, TAN AND FIRM; CUT SURFACE EXUDES A DARK BROWN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #3; LEFT PERINEAL REGION: SINGLE MASS, 28 X 20 X 15 MM, DARK RED, TAN AND FIRM; CUT SURFACE EXUDES A DARK RED SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 21) 3034 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1427 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91336 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 335.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:10 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, AXILLARY (AX) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3035 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1428 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91337 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 262.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:38 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0311 .0118 % .0157 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0318 .0121 % .0161 WEIGHT TAKEN BRAIN (BR) 1.9778 .7526 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.1313 .4305 % .5720 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.1432 .4350 % .5780 WEIGHT TAKEN LIVER (LI) 11.4262 4.3479 % 5.7772 WEIGHT TAKEN LUNG (LU) 1.0703 .4073 % .5412 WEIGHT TAKEN LEFT OVARY (OV0) .0514 .0196 % .0260 WEIGHT TAKEN RIGHT OVARY (OV1) .0622 .0237 % .0314 WEIGHT TAKEN SPLEEN (SP) .4922 .1873 % .2489 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0106 .0040 % .0054 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0102 .0039 % .0052 WEIGHT TAKEN UTERUS (UT) .5673 .2159 % .2868 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 3036 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1429 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91337 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 262.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:38 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3037 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1430 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91338 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 310.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:26 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -DIFFUSELY DARK; ALL LOBES: DARK BROWN -DEGENERATION, CYSTIC,-SLIGHT, FOCAL -RED FOCUS(I)/AREA(S); ALL LOBES: -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- MULTIPLE DARK RED AREAS, UP TO 5 MM IN FOCAL DIAMETER; COLLECTED ON ROUTINE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- SECTIONS FOCAL -TELANGIECTASIS,-MINIMAL, FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -CYST,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, MULTI-FOCAL CERVIX (CV) : CERVIX (CV) : -MASS(ES); EXTENDING INTO LUMEN OF -B-POLYP, ENDOMETRIAL STROMAL,-NEOPLASM BOTH UTERINE HORNS: SINGLE MASS, 30 X PRESENT 10 X 9 MM, RED, DARK RED, TAN, GRAY, AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 20) UTERUS (UT) : -DILATATION,-MODERATELY SEVERE, FOCAL 3038 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1431 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91338 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 310.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:26 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: -B-POLYP, ENDOMETRIAL, STROMAL,- BROWN FLUID NEOPLASM PRESENT -MASS(ES); WITHIN LUMEN OF LEFT HORN: SINGLE MASS, 25 X 20 X 10 MM, TAN, RED, LOBULATED AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 20) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3039 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1432 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91338 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 310.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:26 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 3040 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1433 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91339 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 290.7 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 12:52 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; YELLOW HAIR COAT; EYE (EY) : EYE (EY) : VERIFIED AT NECROPSY: NOT OBSERVED >NOTE:>TRIMMING COMMENT: ONE EYE -ONE EXAMINED,-PRESENT >SKIN & PELAGE; RED HAIR COAT; VENTRAL- MISSING ABDOMINAL; VERIFIED AT NECROPSY: BRAIN (BR) : BRAIN (BR) : OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >DISCHARGE; NASAL; RED IN COLOR; -DILATATION, VENTRICULAR,-PRESENT VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -DARK FOCUS(I)/AREA(S); RIGHT MEDIAN -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NECROPSY: NOT OBSERVED AND LEFT LATERAL LOBES: MULTIPLE DARK FOCAL >EXCRETION; FEW FECES; VERIFIED AT BROWN AREAS, UP TO 5 MM IN DIAMETER; -VACUOLATION, HEPATOCELLULAR,-MODERATE, NECROPSY: NOT APPLICABLE COLLECTED ON ROUTINE SECTIONS MULTI-FOCAL KIDNEY (KD) : KIDNEY (KD) : -CYST(S); RIGHT, CORTICAL SURFACE: -CAST, PROTEINACEOUS,-MINIMAL, MULTI- SINGLE CLEAR FLUID FILLED CYST, 3 MM FOCAL IN DIAMETER -CYST,-MODERATE, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL -THROMBUS,-MINIMAL, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 5 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL TAN AND SOFT -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 3041 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1434 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91339 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 290.7 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 12:52 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE -EDEMA,-MODERATE, FOCAL BROWN ULCERATED AREA, 5 MM IN DIAMETER -EROSION,-MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN >UNREMARKABLE AND SOFT; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; DRY DARK RED MATERIAL -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL REGION: DRY DARK RED MATERIAL -------------------------------------------- 3042 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1435 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91339 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 290.7 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 12:52 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3043 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1436 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91340 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 286.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:29 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT EYE (EY) : NECROPSY: OBSERVED -VASCULARIZATION, CORNEAL,-SLIGHT, >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK FOCAL 67; 60 X 55MM APPEARS TO HAVE MIGRATED BRAIN (BR) : BRAIN (BR) : FROM VENTRAL THORACIC; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT OBSERVED -DIFFUSELY DARK; ALL LOBES: DARK BROWN -FOCUS, ALTERED HEPATOCELLULAR, >EXCRETION; FEW FECES; VERIFIED AT EOSINOPHILIC,-MODERATE, FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -MINERALIZATION, PELVIC,-SLIGHT, MULTI- NECROPSY: NOT APPLICABLE FOCAL ADRENAL, CORTEX (AC) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -HYPERPLASIA, CORTICAL,-MODERATE, FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: TWO CLEAR FLUID -CYST,-PRESENT FILLED CYSTS, EACH IS 7 MM IN DIAMETER PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 5 MM, DARK RED, -CYST,-PRESENT TAN, GRAY, AND SOFT -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-MODERATE, MULTI-FOCAL 3044 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1437 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91340 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 286.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:29 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK RED AREAS, UP TO 4 X 2 MM SKIN (SK) : >NOTE:>COLLECTED ON RIGHT SIDE DUE TO MAMMARY GLAND MASS MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-SEVERE, FOCAL SINGLE MASS, 55 X 45 X 17 MM, TAN, -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT GRAY, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; RIGHT INGUINAL REGION: TAN AND FIRM; COLLECTED ON ROUTINE SECTION >NOTE:>COLLECTED ON RIGHT SIDE DUE TO MAMMARY GLAND MASS VAGINA (VA) : >TISSUE MISSING CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DARK RED, DRY MATERIAL -MALOCCLUSION; BILATERAL, UPPER INCISORS -BONE MARROW SMEAR TAKEN -------------------------------------------- 3045 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1438 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91340 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 286.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:29 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3046 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1439 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91341 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 426.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:28 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL; WEEK 73; LIVER (LI) : 60 X 40MM; VERIFIED AT NECROPSY: -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- OBSERVED FOCAL >SKIN & PELAGE; SORE/SCAB; VENTRAL- -VACUOLATION, HEPATOCELLULAR,-MINIMAL, CERVICAL; VERIFIED AT NECROPSY: MULTI-FOCAL OBSERVED LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 5 X 4 MM; APPEARS SWOLLEN >UNREMARKABLE HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL 3047 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1440 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91341 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 426.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:28 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL -ULCERATION,-SEVERE, FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS #2; VENTRAL CERVICAL REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL SINGLE MASS, 90 X 45 X 30 MM, RED, -HYPERPLASIA, GLANDULAR,-SLIGHT, MULTI- DARK RED, TAN, FIRM, VASCULAR AND FOCAL LOBULATED; SOME SECTIONS EXUDE A DARK -B-FIBROADENOMA,-NEOPLASM PRESENT GREEN SEMIFLUID; COLLECTED; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE DARK RED AND MOIST CRUSTED AREA, COLLECTED INTACT WITH MASS UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS UP TO 9 X 4 -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, X 3 MM (CAP 20) MULTI-FOCAL GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3048 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1441 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91341 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 426.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:28 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3049 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1442 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91342 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 430.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:25 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -MOTTLED; ALL LOBES: RED, TAN, AND -FOCUS, ALTERED HEPATOCELLULAR, BROWN EOSINOPHILIC,-SLIGHT, FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT OVARY (OV) : -CYST,-PRESENT LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, MACROPHAGE, ALVEOLAR,- MULTIPLE TAN AREAS, UP TO 10 X 10 MM SLIGHT, MULTI-FOCAL -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL 3050 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1443 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91342 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 430.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:25 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -B-ADENOMA, C-CELL,- 2 NEOPLASMS PRESENT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM >UNREMARKABLE CERVIX (CV) : CERVIX (CV) : -LARGE; 9 MM IN DIAMETER >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3051 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1444 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91342 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 430.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:25 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3052 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1445 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91343 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 272.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 14:03 PROSECTOR: JODI THIEREN RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0457 .0168 % .0230 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0379 .0139 % .0191 WEIGHT TAKEN BRAIN (BR) 1.9894 .7293 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.4136 .5182 % .7106 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.4822 .5433 % .7450 WEIGHT TAKEN LIVER (LI) 13.1212 4.8098 % 6.5956 WEIGHT TAKEN LUNG (LU) 1.7758 .6510 % .8926 WEIGHT TAKEN LEFT OVARY (OV0) .0724 .0265 % .0364 WEIGHT TAKEN RIGHT OVARY (OV1) .0606 .0222 % .0305 WEIGHT TAKEN SPLEEN (SP) .6191 .2269 % .3112 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0103 .0038 % .0052 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0193 .0071 % .0097 WEIGHT TAKEN UTERUS (UT) 1.0097 .3701 % .5075 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL -STASIS, BILE,-SLIGHT, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL 3053 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1446 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91343 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 272.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 14:03 PROSECTOR: JODI THIEREN RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, MACROPHAGE, ALVEOLAR,- MULTIPLE TAN AREAS, UP TO 4 X 3 MM SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE >UNREMARKABLE DARK BROWN FOCUS, 1 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE -FIBROSIS,-SLIGHT, MULTI-FOCAL NEAR RIGHT HORN OF UTERUS: SINGLE -NECROSIS, FAT,-PRESENT MASS, 20 X 12 X 6 MM, RED, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (LUMBAR) NORMAL (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3054 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1447 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91343 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 272.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 14:03 PROSECTOR: JODI THIEREN RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 3055 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1448 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91344 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 298.3 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:43 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; LIVER (LI) : LIVER (LI) : WEEK 94; 60 X 50MM; VERIFIED AT -CYST(S); ALONG MARGIN OF LEFT LATERAL -CYST,-MINIMAL, FOCAL NECROPSY: OBSERVED LOBE: MULTIPLE CLEAR FLUID FILLED -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- >APPEARANCE; THIN; VERIFIED AT CYSTS, UP TO 1 MM IN DIAMETER; FOCAL NECROPSY: OBSERVED COLLECTED ON ROUTINE SECTION -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >SKIN & PELAGE; BROWN HAIR COAT; FOCAL PERINEAL AREA; VERIFIED AT NECROPSY: KIDNEY (KD) : KIDNEY (KD) : OBSERVED -CYST(S); RIGHT, NEAR CAUDAL POLE: >UNREMARKABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED SINGLE CLEAR FLUID FILLED CYST, 2 MM AT NECROPSY: NOT APPLICABLE IN DIAMETER >APPEARANCE; HEAD TILT; LEFT; VERIFIED LN, MESENTERIC (MS) : AT NECROPSY: NOT APPLICABLE >TISSUE MISSING >EXCRETION; FEW FECES; VERIFIED AT OVARY (OV) : OVARY (OV) : NECROPSY: NOT APPLICABLE -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT >APPEARANCE; HUNCHED; VERIFIED AT FILLED CYST, 6 MM IN DIAMETER NECROPSY: NOT APPLICABLE LUNG (LU) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -MINERALIZATION, VASCULAR,-MINIMAL, NECROPSY: NOT APPLICABLE FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND FIRM MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT 3056 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1449 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91344 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 298.3 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:43 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 52 X 45 X 24 MM, TAN, LOBULATED, VASCULAR AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED VAGINA (VA) : >TISSUE MISSING CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL -BLOOD FILM TAKEN -DISCHARGE-NASAL; DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 3057 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1450 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91344 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 298.3 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:43 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3058 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1451 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91345 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 185.6 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:58 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.8249 4.2160 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3059 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1452 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91345 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 185.6 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:58 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 3060 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1453 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91346 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 06/11/99 STUDY DAY OF DEATH: 502 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 272.8 GRAMS DATE AND TIME OF NECROPSY: 06/11/99 9:54 PROSECTOR: MICHELE MARGGI RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; HEAD- BRAIN (BR) : BRAIN (BR) : ENTIRE; VERIFIED AT NECROPSY: NOT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; RED HAIR COAT; VENTRAL- LIVER (LI) : LIVER (LI) : ABDOMINAL; VERIFIED AT NECROPSY: -RED FOCUS(I)/AREA(S); ALL LOBES: -VACUOLATION, HEPATOCELLULAR,-MINIMAL, OBSERVED MULTIPLE DARK RED AREAS, UP TO 4 X 3 MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT MM; COLLECTED ON ROUTINE SECTIONS NECROPSY: NOT APPLICABLE KIDNEY (KD) : >EXCRETION; FEW FECES; VERIFIED AT -CAST, PROTEINACEOUS,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >EYES; CLEAR DISCHARGE; EYES; VERIFIED -PYELONEPHRITIS,-MINIMAL, MULTI-FOCAL AT NECROPSY: NOT OBSERVED OVARY (OV) : OVARY (OV) : >APPEARANCE; HUNCHED; VERIFIED AT -CYST(S); BILATERAL: SINGLE CLEAR -CYST,-PRESENT NECROPSY: NOT APPLICABLE FLUID FILLED CYST ON EACH, LEFT IS 2 >APPEARANCE; THIN; VERIFIED AT MM IN DIAMETER, RIGHT IS 4 MM IN NECROPSY: OBSERVED DIAMETER THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 9 X 8 MM, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL RED, DARK RED, TAN, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT 3061 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1454 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91346 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 06/11/99 STUDY DAY OF DEATH: 502 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 272.8 GRAMS DATE AND TIME OF NECROPSY: 06/11/99 9:54 PROSECTOR: MICHELE MARGGI RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK BROWN FOCI, PINPOINT AND UP TO 1 MM IN DIAMETER CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW MOIST MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED DRY MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DARK RED AND BROWN CRUSTED MATERIAL -------------------------------------------- 3062 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1455 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91346 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 06/11/99 STUDY DAY OF DEATH: 502 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 272.8 GRAMS DATE AND TIME OF NECROPSY: 06/11/99 9:54 PROSECTOR: MICHELE MARGGI RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3063 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1456 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91347 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 290.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:55 PROSECTOR: KIMBERLY DURLAND RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0292 .0100 % .0138 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0302 .0104 % .0143 WEIGHT TAKEN BRAIN (BR) 2.1108 .7261 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.4951 .5143 % .7083 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.5305 .5265 % .7251 WEIGHT TAKEN LIVER (LI) 16.7366 5.7573 % 7.9290 WEIGHT TAKEN LUNG (LU) 1.5430 .5308 % .7310 WEIGHT TAKEN LEFT OVARY (OV0) .0923 .0318 % .0437 WEIGHT TAKEN RIGHT OVARY (OV1) .1046 .0360 % .0496 WEIGHT TAKEN SPLEEN (SP) .3956 .1361 % .1874 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0154 .0053 % .0073 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0085 .0029 % .0040 WEIGHT TAKEN UTERUS (UT) 1.5954 .5488 % .7558 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); LEFT LATERAL -FOCUS, ALTERED HEPATOCELLULAR, LOBE: SINGLE TAN, SLIGHTLY RAISED EOSINOPHILIC,-MODERATELY SEVERE, FOCAL FOCUS, 3 MM IN DIAMETER; COLLECTED ON -HYPERTROPHY, CENTRILOBULAR, ROUTINE SECTION HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT STOMACH, GL (ST) : -EROSION,-SLIGHT, FOCAL 3064 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1457 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91347 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 290.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:55 PROSECTOR: KIMBERLY DURLAND RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, PINPOINT AND UP TO 3 X 1 MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3065 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1458 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91347 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 290.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:55 PROSECTOR: KIMBERLY DURLAND RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3066 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1459 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91348 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 257.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:23 PROSECTOR: TONY HANFELD RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0446 .0173 % .0219 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0342 .0133 % .0168 WEIGHT TAKEN BRAIN (BR) 2.0387 .7920 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.1265 .4376 % .5526 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.1467 .4455 % .5625 WEIGHT TAKEN LIVER (LI) 17.0849 6.6375 % 8.3803 WEIGHT TAKEN LUNG (LU) 1.7072 .6632 % .8374 WEIGHT TAKEN LEFT OVARY (OV0) .0846 .0329 % .0415 WEIGHT TAKEN RIGHT OVARY (OV1) .1174 .0456 % .0576 WEIGHT TAKEN SPLEEN (SP) .5881 .2285 % .2885 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0100 .0039 % .0049 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0073 .0028 % .0036 WEIGHT TAKEN UTERUS (UT) .7012 .2724 % .3439 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY RED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 3067 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1460 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91348 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 257.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:23 PROSECTOR: TONY HANFELD RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES, -INFILTRATE, MACROPHAGE, ALVEOLAR,- DIFFUSE: MULTIPLE TAN AREAS, UP TO 5 X SLIGHT, MULTI-FOCAL 2 MM -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT POSTERIOR >UNREMARKABLE ASPECT: SINGLE DARK RED AREA, 1 MM IN DIAMETER STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE, PINPOINT FOCI CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -BONE MARROW SMEAR TAKEN -------------------------------------------- 3068 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1461 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91348 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 257.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:23 PROSECTOR: TONY HANFELD RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3069 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1462 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91349 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/11/99 STUDY DAY OF DEATH: 410 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/11/99 15:09 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -DIFFUSELY DARK; ALL LOBES: BROWN -HEMATOPOIESIS, EXTRAMEDULLARY,- >SKIN & PELAGE; YELLOW HAIR COAT; MINIMAL, MULTI-FOCAL VERIFIED AT NECROPSY: NOT OBSERVED -NECROSIS, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT, DIFFUSE LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -MOTTLED; DIFFUSE: RED AND TAN >UNREMARKABLE THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : LUNG (LU) : -UNCOLLAPSED; ALL LOBES >UNREMARKABLE CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-NASAL; DARK RED MOIST MATERIAL -MALOCCLUSION; BILATERAL: UPPER INCISORS -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE AND TAIL, DRY BROWN AND RED MATERIAL -------------------------------------------- 3070 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1463 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91349 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/11/99 STUDY DAY OF DEATH: 410 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/11/99 15:09 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3071 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1464 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91350 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 178.0 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:37 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 6.4865 3.6441 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3072 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1465 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91350 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 178.0 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:37 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 3073 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1466 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91351 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 374.4 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 9:58 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 84; 15 X 15MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE OBSERVED LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; PALE; ENTIRE BODY; -LIGHT FOCUS(I)/AREA(S); VISCERAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- VERIFIED AT NECROPSY: NOT APPLICABLE SURFACE OF LEFT LATERAL AND AT FOCAL >RESPIRATION; LABORED; VERIFIED AT JUNCTION OF MEDIAN LOBES: MULTIPLE TAN -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE AREAS, UP TO 3 MM IN DIAMETER; FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT COLLECTED ON ROUTINE SECTIONS -VACUOLATION, HEPATOCELLULAR,-MODERATE, NECROPSY: NOT APPLICABLE FOCAL >SKIN & PELAGE; SORE/SCAB; LATERAL- -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM THORACIC-RIGHT; VERIFIED AT NECROPSY: PRESENT OBSERVED ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, FOCAL -HEMORRHAGE,-MINIMAL, FOCAL THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 5 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT MAMMARY, FEMALE (MF) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT 3074 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1467 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91351 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 374.4 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 9:58 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 21 X 19 X 12 MM, RED, DARK RED, TAN AND SOFT; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE DARK RED, MOIST ULCERATED AREA, 13 X 11 X 5 MM; COLLECTED INTACT WITH MASS -THICKENED; ALL REGIONS, DIFFUSE: TAN; COLLECTED ON ROUTINE SECTION UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: >UNREMARKABLE CLEAR FLUID CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; LEFT: DARK RED (CAP >UNREMARKABLE 23) LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; RIGHT: RED AND TAN (CAP 22) -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -DIFFUSELY RED; LEFT: DARK RED (CAP -INFILTRATE, MACROPHAGE, PIGMENTED,- 22) MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3075 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1468 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91351 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 374.4 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 9:58 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3076 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1469 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91352 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 383.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:00 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0393 .0102 % .0176 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0309 .0081 % .0138 WEIGHT TAKEN BRAIN (BR) 2.2330 .5821 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.6710 .4356 % .7483 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.7530 .4570 % .7850 WEIGHT TAKEN LIVER (LI) 24.5163 6.3911 % 10.9791 WEIGHT TAKEN LUNG (LU) 1.6006 .4173 % .7168 WEIGHT TAKEN LEFT OVARY (OV0) .0683 .0178 % .0306 WEIGHT TAKEN RIGHT OVARY (OV1) .0809 .0211 % .0362 WEIGHT TAKEN SPLEEN (SP) .6160 .1606 % .2759 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0146 .0038 % .0065 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0174 .0045 % .0078 WEIGHT TAKEN UTERUS (UT) 1.3902 .3624 % .6226 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL, FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL -STASIS, BILE,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL 3077 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1470 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91352 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 383.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:00 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 5 MM, DARK RED, TAN -HEMORRHAGE,-MODERATE, MULTI-FOCAL AND SOFT -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3078 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1471 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91352 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 383.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:00 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3079 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1472 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91353 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 316.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:49 PROSECTOR: WENDY CHERRY RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0281 .0089 % .0117 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0313 .0099 % .0130 WEIGHT TAKEN BRAIN (BR) 2.4047 .7600 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.4217 .4493 % .5912 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.4900 .4709 % .6196 WEIGHT TAKEN LIVER (LI) 17.4901 5.5278 % 7.2733 WEIGHT TAKEN LUNG (LU) 1.7296 .5466 % .7193 WEIGHT TAKEN LEFT OVARY (OV0) .0924 .0292 % .0384 WEIGHT TAKEN RIGHT OVARY (OV1) .1090 .0345 % .0453 WEIGHT TAKEN SPLEEN (SP) .5484 .1733 % .2281 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0170 .0054 % .0071 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0262 .0083 % .0109 WEIGHT TAKEN UTERUS (UT) .7222 .2283 % .3003 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; OVARY (OV) : OVARY (OV) : VERIFIED AT NECROPSY: OBSERVED -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 3 MM IN DIAMETER THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE BLACK AREAS, UP TO 2 X 1 MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3080 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1473 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91353 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 316.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:49 PROSECTOR: WENDY CHERRY RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TAIL (TI) : TAIL (TI) : -RAISED AREA(S); ALONG ENTIRE LENGTH: -CYST, EPIDERMAL INCLUSION,-PRESENT MULTIPLE BROWN AREAS, UP TO 4 X 3 X 2 -INFLAMMATION, ACUTE,-MODERATELY MM; SOME CONTAIN A GREEN, CASEOUS SEVERE, MULTI-FOCAL SEMISOLID; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3081 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1474 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91353 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 316.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:49 PROSECTOR: WENDY CHERRY RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3082 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1475 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91354 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 211.9 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 11:27 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; RED HAIR COAT; LIMBS- LIVER (LI) : FRONT; VERIFIED AT NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >BEHAVIOR; HYPOACTIVE; VERIFIED AT EOSINOPHILIC,-SEVERE, FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >APPEARANCE; HUNCHED; VERIFIED AT -MINERALIZATION, PELVIC,-SLIGHT, MULTI- NECROPSY: NOT APPLICABLE FOCAL >SKIN & PELAGE; YELLOW HAIR COAT; ADRENAL, CORTEX (AC) : VERIFIED AT NECROPSY: OBSERVED -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, MACROPHAGE, ALVEOLAR,- MULTIPLE TAN AREAS, UP TO 5 MM IN SLIGHT, MULTI-FOCAL DIAMETER PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 8 X 4 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL STOMACH, GL (ST) : -EDEMA,-MODERATE, DIFFUSE 3083 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1476 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91354 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 211.9 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 11:27 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE -EDEMA,-MODERATE, DIFFUSE TAN, WHITE ERODED AREA, 5 MM IN -HEMORRHAGE,-MINIMAL, MULTI-FOCAL DIAMETER -INFLAMMATION, ACUTE,-MINIMAL, MULTI- FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MEDIASTINAL (ML) : -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; RED, DRY MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE AND FRONT LIMBS: YELLOW AND MOIST MATERIAL AND RED, DRY MATERIAL -ANIMAL THIN -------------------------------------------- 3084 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1477 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91354 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 211.9 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 11:27 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3085 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1478 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91355 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 253.0 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:08 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0352 .0139 % .0187 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0304 .0120 % .0162 WEIGHT TAKEN BRAIN (BR) 1.8820 .7439 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.1065 .4374 % .5879 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.1411 .4510 % .6063 WEIGHT TAKEN LIVER (LI) 10.1791 4.0234 % 5.4087 WEIGHT TAKEN LUNG (LU) 1.1450 .4526 % .6084 WEIGHT TAKEN LEFT OVARY (OV0) .0538 .0213 % .0286 WEIGHT TAKEN RIGHT OVARY (OV1) .0520 .0206 % .0276 WEIGHT TAKEN SPLEEN (SP) .3082 .1218 % .1638 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0109 .0043 % .0058 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0082 .0032 % .0044 WEIGHT TAKEN UTERUS (UT) .5800 .2292 % .3082 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; OVARY (OV) : VERIFIED AT NECROPSY: OBSERVED -HEMORRHAGE,-MODERATE, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 3086 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1479 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91355 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 253.0 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:08 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS) ------------------------------------------------------------------------------------------------------------------------------------ 3087 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1480 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91356 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 280.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:19 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: >UNREMARKABLE >APPEARANCE; THIN; VERIFIED AT MULTIPLE DARK RED AREAS, UP TO 2 X 1 NECROPSY: OBSERVED MM; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : KIDNEY (KD) : -DIFFUSELY LIGHT; BILATERAL: TAN -MINERALIZATION, PELVIC,-MINIMAL, MULTI- -CYST(S); BILATERAL, DIFFUSE: MULTIPLE FOCAL CLEAR FLUID FILLED CYSTS, UP TO 2 MM -NEPHROPATHY, CHRONIC PROGRESSIVE,- IN DIAMETER SEVERE, DIFFUSE PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE GREEN FLUID -MINERALIZATION,-SLIGHT, MULTI-FOCAL FILLED CYST, 5 MM IN DIAMETER -PIGMENTED,-MODERATELY SEVERE, FOCAL -THROMBUS,-SEVERE, FOCAL THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 3088 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1481 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91356 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 280.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:19 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -RED FOCUS(I)/AREA(S); ALL REGIONS, DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM -LARGE; 7 X 6 X 4 MM MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT UTERUS (UT) : UTERUS (UT) : -CYST(S); WITHIN WALLS OF RIGHT HORN: -DILATATION,-MODERATE, FOCAL MULTIPLE CLEAR FLUID FILLED CYSTS, UP -B-POLYP, ENDOMETRIAL, STROMAL,- TO 3 MM IN DIAMETER NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3089 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1482 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91356 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 280.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:19 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3090 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1483 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91357 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 298.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:12 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -HYPERPLASIA, BILE DUCT,-SLIGHT, MULTI- MULTIPLE DARK RED AREAS, UP TO 3 MM IN FOCAL DIAMETER; COLLECTED ON ROUTINE -HYPERTROPHY, CENTRILOBULAR, SECTIONS HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 10 MM IN DIAMETER (RIGHT -DEGENERATION, CYSTIC, CORTICAL,- IS 4 X 3 X 3 MM) SEVERE, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 4 MM IN DIAMETER BONE, STERNUM (SB) : -HYPERPLASIA, CARTILAGE,-MODERATE, FOCAL THYMUS (TH) : -CYST,-PRESENT 3091 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1484 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91357 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 298.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:12 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, MACROPHAGE, ALVEOLAR,- MULTIPLE TAN AREAS, UP TO 6 X 5 MM MODERATE, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); MID ASPECT: -CYST,-PRESENT SINGLE DARK RED, TAN AND SLIGHTLY -HEMORRHAGE,-MODERATE, MULTI-FOCAL RAISED AREA, 2 MM IN DIAMETER -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE >UNREMARKABLE TAN ERODED AREAS, UP TO 3 X 1 MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY -------------------------------------------- 3092 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1485 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91357 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 298.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:12 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3093 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1486 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91358 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/02/99 STUDY DAY OF DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/02/99 9:30 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK EYE (EY) : 61; 50 X 40MM; VERIFIED AT NECROPSY: -INFLAMMATION, ACUTE,-MODERATE, FOCAL OBSERVED SPLEEN (SP) : >SKIN & PELAGE; YELLOW HAIR COAT; -PIGMENT, INCREASED,-MINIMAL, MULTI- VERIFIED AT NECROPSY: OBSERVED FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT OVARY (OV) : NECROPSY: NOT APPLICABLE -PIGMENTED,-MINIMAL, MULTI-FOCAL >SKIN & PELAGE; COLD TO TOUCH; ENTIRE THYMUS (TH) : BODY; VERIFIED AT NECROPSY: NOT -CYST,-PRESENT APPLICABLE LUNG (LU) : >EXCRETION; FEW FECES; VERIFIED AT -N-MAMMARY CARCINOMA,-NEOPLASM PRESENT NECROPSY: NOT APPLICABLE PITUITARY (PI) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -PIGMENT,-MINIMAL, MULTI-FOCAL NECROPSY: OBSERVED -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EDEMA,-MODERATE, MULTI-FOCAL -EROSION,-MODERATE, MULTI-FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 70 X 35 X 30 MM, RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL DARK RED, TAN, LOBULATED AND FIRM; -NECROSIS,-MODERATE, MULTI-FOCAL SAME ON CUT SURFACE; COLLECTED; -M-CARCINOMA,-NEOPLASM PRESENT REGIONAL LN (MANDIBULAR) NOT IDENTIFIED 3094 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1487 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91358 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/02/99 STUDY DAY OF DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/02/99 9:30 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MISSING/NOT IDENTIFIED; NOT >NOT REQUIRED TO BE EXAMINED FOR ANIMAL IDENTIFIED DUE TO MASS LN, MEDIASTINAL (ML) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY YELLOW MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY MATERIAL -------------------------------------------- 3095 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1488 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91358 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/02/99 STUDY DAY OF DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/02/99 9:30 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3096 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1489 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91359 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/25/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/25/99 10:51 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -MOTTLED; ALL LOBES: DARK BROWN AND -HEMATOPOIESIS, EXTRAMEDULLARY,- TAN MINIMAL, MULTI-FOCAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL, MULTI-FOCAL -PIGMENT, KUPFFER CELL,-MINIMAL, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -MISSING/NOT IDENTIFIED; LEFT: MISSING -ONE EXAMINED,-PRESENT AT TIME OF NECROPSY PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-SEVERE, DIFFUSE 3097 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1490 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91359 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/25/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/25/99 10:51 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : UTERUS (UT) : -LARGE, DIFFUSE; BILATERAL, DIFFUSE: -HEMORRHAGE,-MINIMAL, MULTI-FOCAL EACH IS 74 X 20 X 20 MM -SEMIFLUID MATERIAL; BILATERAL, WITHIN LUMEN: LARGE AMOUNTS OF DARK BROWN MUCOID SEMIFLUID MATERIAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT -------------------------------------------- 3098 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1491 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91359 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/25/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/25/99 10:51 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3099 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1492 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91360 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 328.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:32 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; HEAD- LIVER (LI) : LIVER (LI) : ENTIRE; VERIFIED AT NECROPSY: OBSERVED -MOTTLED; ALL LOBES: RED AND TAN -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATELY SEVERE, FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: MULTIPLE CLEAR -CYST,-PRESENT FLUID FILLED CYSTS ON EACH, UP TO 2 MM IN DIAMETER BONE, STERNUM (SB) : -HYPERPLASIA, CARTILAGE,-MODERATE, FOCAL THYMUS (TH) : -CYST,-PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 3100 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1493 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91360 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 328.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:32 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: DRY RED MATERIAL -BONE MARROW SMEAR TAKEN -------------------------------------------- 3101 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1494 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91360 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 328.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:32 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3102 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1495 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91361 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 338.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:07 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -MASS(ES); LEFT LATERAL LOBE: SINGLE -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- MASS, 15 MM IN DIAMETER, RED, BROWN, FOCAL TAN, AND FIRM; SAME ON CUT SURFACE; -HYPERTROPHY, CENTRILOBULAR, COLLECTED ON ROUTINE SECTION; REGIONAL HEPATOCELLULAR,-SLIGHT, DIFFUSE LN (HEPATIC) NOT POSITIVELY IDENTIFIED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- (CAP 20) FOCAL -VACUOLATION, HEPATOCELLULAR,-SEVERE, FOCAL -M-CARCINOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MODERATE, FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: LEFT HAS A SINGLE -HEMORRHAGE,-SEVERE, MULTI-FOCAL CLEAR FLUID FILLED CYST, 2 MM IN -PIGMENTED,-MODERATE, MULTI-FOCAL DIAMETER, RIGHT HAS A SINGLE RED FLUID -THROMBUS,-SEVERE, FOCAL FILLED CYST, 7 MM IN DIAMETER -B-GRANULOSA/THECA CELL TUMOR,-NEOPLASM PRESENT 3103 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1496 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91361 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 338.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:07 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, OTHER (LN) : >TISSUE MISSING >NOTE:>HEPATIC LYMPH NODE NOT PRESENT ON SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS -BONE MARROW SMEAR TAKEN -------------------------------------------- 3104 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1497 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91361 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 338.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:07 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3105 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1498 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91362 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 644.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:27 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK LIVER (LI) : LIVER (LI) : 85; 90 X 65MM; VERIFIED AT NECROPSY: -LIGHT FOCUS(I)/AREA(S); AT JUNCTION -FOCUS, ALTERED HEPATOCELLULAR, OBSERVED OF RIGHT AND LEFT MEDIAN LOBES: SINGLE EOSINOPHILIC,-MODERATE, FOCAL >SKIN & PELAGE; RED HAIR COAT; HEAD- TAN AREA, 4 X 3 MM; COLLECTED ON -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- ENTIRE; VERIFIED AT NECROPSY: NOT ROUTINE SECTION FOCAL OBSERVED -MOTTLED; ALL LOBES: RED, TAN AND -VACUOLATION, HEPATOCELLULAR,-MINIMAL, >MASS(ES); AXILLARY REGION-RIGHT; WEEK BROWN MULTI-FOCAL 89; 50 X 40MM; VERIFIED AT NECROPSY: -MASS(ES); DIAPHRAGMATIC SURFACE OF -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM OBSERVED LEFT LATERAL LOBE: SINGLE MASS, 14 X PRESENT 14 X 3 MM, RED AND FIRM; SAME ON CUT SURFACE; COLLECTED ON ROUTINE SECTION; REGIONAL LN (HEPATIC) NOT IDENTIFIED KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL OVARY (OV) : -MINERALIZATION,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 5 X 5 X 3 MM; APPEARS SWOLLEN -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 3106 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1499 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91362 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 644.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:27 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 40 X 35 X 14 MM, RED, -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT TAN, SOFT AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 90 X 70 X 55 MM (185.3 GRAMS) RED, DARK RED, TAN, FIRM, VASCULAR AND LOBULATED; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN (CAP -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3107 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1500 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91362 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 644.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:27 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 3108 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1501 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91363 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 295.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:35 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -DIFFUSELY LIGHT; ALL LOBES: TAN -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- >HOUSING,FOOD,WATER & MAINT.; TEETH FOCAL CUT; VERIFIED AT NECROPSY: NOT -VACUOLATION, HEPATOCELLULAR,-MINIMAL, APPLICABLE MULTI-FOCAL >SKIN & PELAGE; BROWN HAIR COAT; KIDNEY (KD) : VENTRAL-ABDOMINAL; VERIFIED AT -CAST, PROTEINACEOUS,-SLIGHT, MULTI- NECROPSY: OBSERVED FOCAL >SKIN & PELAGE; RED HAIR COAT; HEAD- -PYELONEPHRITIS,-SLIGHT, MULTI-FOCAL ENTIRE; VERIFIED AT NECROPSY: NOT SPLEEN (SP) : SPLEEN (SP) : OBSERVED -LIGHT FOCUS(I)/AREA(S); NEAR EACH >UNREMARKABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT POLE: MULTIPLE GREY AREAS, UP TO 12 X NECROPSY: NOT APPLICABLE 5 MM >APPEARANCE; THIN; VERIFIED AT LN, MESENTERIC (MS) : NECROPSY: NOT OBSERVED -DILATATION, SINUSOIDAL,-MINIMAL, MULTI- >RESPIRATION; AUDIBLE; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -CYST,-PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EROSION,-MODERATE, FOCAL MULTIPLE BROWN AREAS, UP TO 3 X 2 MM VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE LIGHT GREEN 3109 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1502 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91363 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 295.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:35 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: COLLECTED -HEMORRHAGE,-SLIGHT, MULTI-FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; EXTENDING INTO ENTIRE ABDOMINAL REGION: DARK YELLOW AND BROWN -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: LOWER INCISORS -------------------------------------------- 3110 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1503 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91363 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 295.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:35 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3111 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1504 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91364 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/02/00 STUDY DAY OF DEATH: 707 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/02/00 9:23 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >DISCHARGE; NASAL; RED IN COLOR; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT -HEMORRHAGE,-MODERATE, FOCAL NECROPSY: OBSERVED ADRENAL, CORTEX (AC) : >SKIN & PELAGE; YELLOW HAIR COAT; -DEGENERATION, CYSTIC, CORTICAL,- VERIFIED AT NECROPSY: OBSERVED SLIGHT, FOCAL >EXCRETION; FEW FECES; VERIFIED AT THYMUS (TH) : NECROPSY: NOT APPLICABLE -CYST,-PRESENT LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); DORSAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- SURFACE OF LEFT LOBE AND RIGHT CAUDAL MINIMAL, FOCAL LOBE: MULTIPLE WHITE AREAS, UP TO 12 X 7 MM THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 14 X 10 X 10 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL SKIN (SK) : -ATROPHY, ADNEXAL,-MODERATE, MULTI- FOCAL 3112 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1505 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91364 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/02/00 STUDY DAY OF DEATH: 707 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/02/00 9:23 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : SKIN (SK) : -ALOPECIA-FOCAL; RIGHT FLANK: SINGLE -INFLAMMATION, CHRONIC,-MODERATELY AREA, 35 X 18 MM; AREA ALSO HAS SEVERE, MULTI-FOCAL MULTIPLE TAN AND DRY CRUSTED AREAS, UP TO 3 X 2 MM; COLLECTED MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DARK YELLOW AND MOIST -DISCHARGE-NASAL; DARK RED AND DRY -ANIMAL THIN -------------------------------------------- 3113 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1506 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91364 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/02/00 STUDY DAY OF DEATH: 707 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/02/00 9:23 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3114 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1507 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91365 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 240.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:59 PROSECTOR: TONY HANFELD RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0314 .0131 % .0151 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0325 .0135 % .0156 WEIGHT TAKEN BRAIN (BR) 2.0802 .8653 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.1270 .4688 % .5418 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.1918 .4958 % .5729 WEIGHT TAKEN LIVER (LI) 10.9570 4.5578 % 5.2673 WEIGHT TAKEN LUNG (LU) 1.4156 .5889 % .6805 WEIGHT TAKEN LEFT OVARY (OV0) .0533 .0222 % .0256 WEIGHT TAKEN RIGHT OVARY (OV1) .0604 .0251 % .0290 WEIGHT TAKEN SPLEEN (SP) .4329 .1801 % .2081 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0257 .0107 % .0124 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0242 .0101 % .0116 WEIGHT TAKEN UTERUS (UT) 1.2583 .5234 % .6049 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR,-MINIMAL, FOCAL HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE >UNREMARKABLE BROWN FOCUS, 1 MM IN DIAMETER UTERUS (UT) : UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE -DILATATION,-MINIMAL, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3115 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1508 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91365 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 240.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:59 PROSECTOR: TONY HANFELD RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3116 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1509 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91366 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 319.5 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 14:27 PROSECTOR: MICHELE MARGGI RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 74; 30 X 30MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); INGUINAL REGION-LEFT; WEEK LIVER (LI) : 80; 35 X 30MM; VERIFIED AT NECROPSY: -VACUOLATION, HEPATOCELLULAR,-MINIMAL, OBSERVED MULTI-FOCAL >SKIN & PELAGE; RED HAIR COAT; HEAD- PANCREAS (PA) : ENTIRE; VERIFIED AT NECROPSY: NOT -ATROPHY, ACINAR,-MINIMAL, FOCAL OBSERVED OVARY (OV) : -HEMORRHAGE,-MINIMAL, FOCAL LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, FOCAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PARATHYROID (PT) : PARATHYROID (PT) : -LARGE; LEFT: 4 MM IN DIAMETER (RIGHT >UNREMARKABLE IS 1 MM IN DIAMETER) PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 10 X 7 -HEMORRHAGE,-MODERATELY SEVERE, MULTI- MM, DARK RED, TAN, GREY AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT 3117 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1510 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91366 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 319.5 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 14:27 PROSECTOR: MICHELE MARGGI RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL SINGLE MASS, 35 X 25 X 15 MM, RED, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL DARK RED, TAN, VASCULAR, AND SOFT; ON -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT SECTION EXUDES A DARK BROWN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 21) -MASS #2; LEFT PERINEUM REGION, EXTENDING TO ANUS: SINGLE MASS, 40 X 30 X 20 MM, TAN, GREY, BROWN, AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (LUMBAR) NORMAL (CAP 22); POSSIBLY CONSISTENT WITH MASS (VENTRAL- ABDOMINAL-LEFT) AS NOTED IN CLINICAL OBSERVATIONS UTERUS (UT) : UTERUS (UT) : -CYST(S); LEFT, WITHIN LUMEN: SINGLE >UNREMARKABLE CLEAR FLUID FILLED CYST, 4 X 2 X 2 MM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT TAIL (TI) : TAIL (TI) : -CRUSTED AREA(S); ALONG ENTIRE LENGTH: -CYST, EPIDERMAL INCLUSION,-PRESENT MULTIPLE DARK BROWN CRUSTED AREAS, UP -INFLAMMATION, ACUTE,-MODERATELY TO 2 X 1 MM; SOME AREAS EXUDE A LIGHT SEVERE, FOCAL GREEN CASEOUS MATERIAL; COLLECTED LN, INGUINAL (IN) : >TISSUE MISSING GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 3118 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1511 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91366 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 319.5 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 14:27 PROSECTOR: MICHELE MARGGI RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 3119 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1512 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91367 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 307.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:16 PROSECTOR: JODI THIEREN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0398 .0129 % .0186 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0388 .0126 % .0182 WEIGHT TAKEN BRAIN (BR) 2.1356 .6938 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.7518 .5691 % .8203 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.8977 .6165 % .8886 WEIGHT TAKEN LIVER (LI) 17.4093 5.6560 % 8.1519 WEIGHT TAKEN LUNG (LU) 1.7508 .5688 % .8198 WEIGHT TAKEN LEFT OVARY (OV0) .0740 .0240 % .0347 WEIGHT TAKEN RIGHT OVARY (OV1) .0761 .0247 % .0356 WEIGHT TAKEN SPLEEN (SP) .5978 .1942 % .2799 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0128 .0042 % .0060 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0122 .0040 % .0057 WEIGHT TAKEN UTERUS (UT) 1.3167 .4278 % .6165 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MINIMAL, MULTI-FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -CYST,-PRESENT UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: >UNREMARKABLE CLEAR FLUID 3120 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1513 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91367 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 307.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:16 PROSECTOR: JODI THIEREN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; BROKEN, UPPER LEFT INCISOR -------------------------------------------- 3121 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1514 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91367 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 307.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:16 PROSECTOR: JODI THIEREN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3122 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1515 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91368 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 270.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 14:14 PROSECTOR: GREGORY CODDINGTON RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0410 .0152 % .0194 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0431 .0159 % .0204 WEIGHT TAKEN BRAIN (BR) 2.1117 .7804 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.2641 .4671 % .5986 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.4674 .5423 % .6949 WEIGHT TAKEN LIVER (LI) 19.0978 7.0576 % 9.0438 WEIGHT TAKEN LUNG (LU) 1.4081 .5204 % .6668 WEIGHT TAKEN LEFT OVARY (OV0) .0759 .0280 % .0359 WEIGHT TAKEN RIGHT OVARY (OV1) .0568 .0210 % .0269 WEIGHT TAKEN SPLEEN (SP) .4751 .1756 % .2250 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0120 .0044 % .0057 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0160 .0059 % .0076 WEIGHT TAKEN UTERUS (UT) 1.0793 .3989 % .5111 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 3123 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1516 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91368 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 270.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 14:14 PROSECTOR: GREGORY CODDINGTON RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3124 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1517 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91369 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 448.4 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:00 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 45; 55 X 55MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); AXILLARY REGION-LEFT; WEEK LIVER (LI) : LIVER (LI) : 63; 20 X 25MM; VERIFIED AT NECROPSY: -LARGE; ALL LOBES: MARGINS APPEAR -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT, OBSERVED ROUNDED MULTI-FOCAL >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK -DIFFUSELY DARK; ALL LOBES: DARK BROWN 67; 20 X 20MM; VERIFIED AT NECROPSY: KIDNEY (KD) : OBSERVED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL SPLEEN (SP) : SPLEEN (SP) : -LARGE; 60 X 13 X 8 MM -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE, MULTI- FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, MULTI-FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 5 MM, RED, DARK -HEMORRHAGE,-MODERATELY SEVERE, MULTI- RED, TAN AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT 3125 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1518 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91369 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 448.4 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:00 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : -ULCERATION,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL SINGLE MASS, 63 X 57 X 45 MM, RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL DARK RED, TAN, BROWN, FIRM AND -INFLAMMATION, ACUTE,-MODERATE, MULTI- LOBULATED; SAME ON CUT SURFACE; FOCAL OVERLYING SKIN HAS MULTIPLE DRY RED -MINERALIZATION,-MODERATE, MULTI-FOCAL AND BROWN CRUSTED AREAS, UP TO 45 X 25 -NECROSIS,-MODERATELY SEVERE, MULTI- MM (CAP 20); REGIONAL LN (AXILLARY) FOCAL NORMAL (CAP 21) -PIGMENT,-SLIGHT, MULTI-FOCAL -MASS #2; RIGHT AXILLARY REGION, -M-CARCINOMA,- 4 NEOPLASMS PRESENT EXTENDS TO VENTRAL THORACIC REGION: SINGLE MASS, 37 X 23 X 12, RED, TAN, BROWN, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 21) -MASS #3; LEFT AXILLARY REGION, CAUDAL TO MASS #2: SINGLE MASS, 20 X 14 X 8 MM, RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (AXILLARY) NORMAL (CAP 21) -MASS #4; LEFT INGUINAL REGION: SINGLE MASS, 33 X 21 X 14 MM, RED, DARK RED, TAN, WHITE, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT 3126 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1519 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91369 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 448.4 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:00 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 3127 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1520 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91370 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 209.2 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 9:25 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0375 .0179 % .0217 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0361 .0173 % .0209 WEIGHT TAKEN BRAIN (BR) 1.7300 .8270 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) .7688 .3675 % .4444 WEIGHT TAKEN RIGHT KIDNEY (KD1) .7898 .3775 % .4565 WEIGHT TAKEN LIVER (LI) 9.3806 4.4840 % 5.4223 WEIGHT TAKEN LUNG (LU) .9689 .4631 % .5601 WEIGHT TAKEN LEFT OVARY (OV0) .0502 .0240 % .0290 WEIGHT TAKEN RIGHT OVARY (OV1) .0563 .0269 % .0325 WEIGHT TAKEN SPLEEN (SP) .4589 .2194 % .2653 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0071 .0034 % .0041 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0066 .0032 % .0038 WEIGHT TAKEN UTERUS (UT) .5249 .2509 % .3034 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 3128 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1521 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91370 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 209.2 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 9:25 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3129 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1522 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91371 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 380.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:01 PROSECTOR: KIMBERLY DURLAND RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0370 .0097 % .0163 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0357 .0094 % .0158 WEIGHT TAKEN BRAIN (BR) 2.2665 .5964 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.8602 .4895 % .8207 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.8759 .4937 % .8277 WEIGHT TAKEN LIVER (LI) 17.1837 4.5220 % 7.5816 WEIGHT TAKEN LUNG (LU) 1.7414 .4583 % .7683 WEIGHT TAKEN LEFT OVARY (OV0) .0631 .0166 % .0278 WEIGHT TAKEN RIGHT OVARY (OV1) .0662 .0174 % .0292 WEIGHT TAKEN SPLEEN (SP) .6717 .1768 % .2964 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0236 .0062 % .0104 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0190 .0050 % .0084 WEIGHT TAKEN UTERUS (UT) 1.4559 .3831 % .6424 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK LIVER (LI) : 52; VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL 3130 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1523 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91371 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 380.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:01 PROSECTOR: KIMBERLY DURLAND RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN AND WHITE CREAMY SEMIFLUID FILLED CYSTS, TO 7 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION; POSSIBLY CONSISTENT WITH MASS (RIGHT AXILLARY REGION) NOTED IN CLINICAL OBSERVATIONS UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY -------------------------------------------- 3131 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1524 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91371 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 380.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:01 PROSECTOR: KIMBERLY DURLAND RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3132 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1525 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91372 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 358.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:16 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT EYE (EY) : NECROPSY: OBSERVED -HYPERPLASIA, CORNEAL EPITHELIUM,- SLIGHT, FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- MULTIPLE AREAS, UP TO 2 MM IN FOCAL DIAMETER; COLLECTED ON ROUTINE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- SECTIONS FOCAL -THROMBUS,-MINIMAL, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 3133 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1526 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91372 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 358.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:16 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 8 X 4 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, TAN, AND FIRM MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN LUMEN: -DILATATION,-MODERATE, DIFFUSE MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS -BONE MARROW SMEAR TAKEN -------------------------------------------- 3134 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1527 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91372 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 358.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:16 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3135 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1528 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91373 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 256.2 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 16:03 PROSECTOR: NANCY DIEDRICH RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; HEAD- BRAIN (BR) : BRAIN (BR) : ENTIRE; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: OBSERVED -HEMORRHAGE,-MODERATE, FOCAL >SKIN & PELAGE; RED HAIR COAT; VENTRAL- LIVER (LI) : LIVER (LI) : ABDOMINAL; VERIFIED AT NECROPSY: -LARGE; ALL LOBES: THICKENED -VACUOLATION, HEPATOCELLULAR,-MODERATE, OBSERVED -MOTTLED; ALL LOBES: DARK BROWN AND MULTI-FOCAL >SKIN & PELAGE; YELLOW HAIR COAT; TAN VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : KIDNEY (KD) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -MOTTLED; BILATERAL: BROWN AND TAN -CAST, PROTEINACEOUS,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >APPEARANCE; HUNCHED; VERIFIED AT SPLEEN (SP) : NECROPSY: NOT APPLICABLE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR >UNREMARKABLE FLUID FILLED CYST ON EACH; EACH IS 8 MM IN DIAMETER PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 8 X 8 MM, RED, DARK -HEMORRHAGE,-MODERATELY SEVERE, MULTI- RED, GRAY AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -EDEMA,-MODERATE, FOCAL TAN ERODED AREAS, UP TO 4 X 2 MM -EROSION,-MODERATELY SEVERE, FOCAL 3136 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1529 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91373 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 256.2 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 16:03 PROSECTOR: NANCY DIEDRICH RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; MUCOSA -INFLAMMATION, ACUTE,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: -GALACTOCELE,-MODERATE, MULTI-FOCAL MULTIPLE TAN, CREAMY SEMIFLUID FILLED -MINERALIZATION,-MINIMAL, MULTI-FOCAL CYSTS, UP TO 5 MM IN DIAMETER (CAP 20) -M-CARCINOMA,-NEOPLASM PRESENT -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM (CAP 21) VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; LIGHT GREEN AND >UNREMARKABLE MUCOID CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: DARK RED AND TAN >UNREMARKABLE (CAP 22) GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOWISH, BROWN AND MOIST -BLOOD FILM TAKEN -DISCHARGE-NASAL; RED AND DRY -STAINS - SKIN/HAIRCOAT; ABDOMEN, CHEST AND HEAD: DARK RED AND DRY -ANIMAL THIN -------------------------------------------- 3137 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1530 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91373 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 256.2 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 16:03 PROSECTOR: NANCY DIEDRICH RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3138 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1531 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91374 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 235.7 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 13:23 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); TAIL-PROXIMAL; BRAIN (BR) : BRAIN (BR) : WEEK 88; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >EYES; RED DISCHARGE; EYE-RIGHT; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR,-MINIMAL, >DISCHARGE; NASAL; RED IN COLOR; MULTI-FOCAL VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : >APPEARANCE; THIN; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: OBSERVED MINIMAL, MULTI-FOCAL >SKIN & PELAGE; YELLOW HAIR COAT; -PYELONEPHRITIS,-MODERATE, MULTI-FOCAL VERIFIED AT NECROPSY: OBSERVED ADRENAL, MEDULLA (MA) : >EXCRETION; NO FECES; VERIFIED AT -VACUOLATION,-SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: MULTIPLE CLEAR -CYST,-PRESENT FLUID FILLED CYSTS, UP TO 4 MM IN -HEMORRHAGE,-MINIMAL, MULTI-FOCAL DIAMETER THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 5 MM, TAN, RED AND -B-ADENOMA,-NEOPLASM PRESENT SOFT 3139 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1532 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91374 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 235.7 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 13:23 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE >UNREMARKABLE TAN ERODED AREAS, UP TO 2 MM IN DIAMETER COLON (CO) : -EDEMA,-SLIGHT, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL UTERUS (UT) : -DILATATION,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT TAIL (TI) : TAIL (TI) : -CRUSTED AREA(S); ALONG ENTIRE TAIL: -CYST, EPIDERMAL INCLUSION,-PRESENT MULTIPLE DRY DARK BROWN AND CRUSTED -INFLAMMATION, ACUTE,-MODERATELY AREAS, UP TO 4 MM IN DIAMETER; SEVERE, FOCAL COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY DARK BROWN MATERIAL -DISCHARGE-NASAL; DRY DARK BROWN MATERIAL -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL REGION: MOIST YELLOW MATERIAL 3140 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1533 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91374 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 235.7 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 13:23 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3141 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1534 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91375 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 272.3 GRAMS DATE AND TIME OF NECROPSY: 08/27/99 8:02 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE -HEMORRHAGE,-MODERATE, FOCAL >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : NECROPSY: NOT OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >APPEARANCE; HUNCHED; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -MINERALIZATION, PELVIC,-MODERATE, NECROPSY: NOT APPLICABLE MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- SEVERE, FOCAL -HEMORRHAGE,-MODERATELY SEVERE, FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 3 MM IN DIAMETER THYROID (TY) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 6 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- TAN, LOBULATED AND SOFT FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 3142 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1535 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91375 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 272.3 GRAMS DATE AND TIME OF NECROPSY: 08/27/99 8:02 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -DARK FOCUS(I)/AREA(S); MUCOSA, NEAR -EDEMA,-MODERATELY SEVERE, DIFFUSE JUNCTION OF GLANDULAR MUCOSA: MULTIPLE -INFLAMMATION, ACUTE,-SLIGHT, FOCAL DARK BROWN AREAS, UP TO 2 X 1 MM -ULCERATION,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY DARK RED MATERIAL -------------------------------------------- 3143 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1536 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91375 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 272.3 GRAMS DATE AND TIME OF NECROPSY: 08/27/99 8:02 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3144 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1537 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91376 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 321.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:42 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 100; 10 X 10MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : LIVER (LI) : >MASS(ES); INGUINAL REGION-LEFT; WEEK -DARK FOCUS(I)/AREA(S); ALL LOBES: -CYST,-MODERATE, FOCAL 105; THREE MASSES UNDER 10MM; VERIFIED MULTIPLE DARK BROWN AREAS, UP TO 5 MM -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- AT NECROPSY: OBSERVED IN DIAMETER; SOME ARE SUNKEN WITH A FOCAL STELLATE PATTERN; COLLECTED ON ROUTINE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- SECTION FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 15 MM IN DIAMETER BONE, STERNUM (SB) : -HYPERPLASIA, CARTILAGE,-MODERATE, FOCAL THYMUS (TH) : -CYST,-PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, GRAY AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-MODERATELY SEVERE, FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT 3145 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1538 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91376 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 321.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:42 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 38 X 27 X 20 MM, RED, DARK RED, BROWN, TAN, FIRM, LOBULATED AND VASCULAR; ON SECTION, SOME AREAS EXUDE A BROWN FLUID; COLLECTED; REGIONAL LN (SEE AXILLARY) -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 30 X 30 X 10 MM, RED, TAN, DARK BROWN, SOFT AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION UTERUS (UT) : UTERUS (UT) : -LARGE, FOCAL; BILATERAL, DISTAL -CYST, KERATIN,-PRESENT PORTION OF EACH HORN: UP TO 12 MM IN -DILATATION,-SEVERE, DIFFUSE DIAMETER -HYPERPLASIA, ENDOMETRIAL,-MODERATE, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP -INFILTRATE, MACROPHAGE, PIGMENTED,- 20) MODERATE, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -FLUID-ABDOMINAL CAVITY; LARGE AMOUNTS OF LIGHT BROWN FLUID 3146 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1539 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91376 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 321.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:42 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3147 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1540 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91377 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/06/99 STUDY DAY OF DEATH: 497 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/06/99 13:17 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK LIVER (LI) : 43; 90 X 85MM; VERIFIED AT NECROPSY: -NECROSIS, HEPATOCELLULAR,-MODERATELY OBSERVED SEVERE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL JEJUNUM (JE) : >AUTOLYTIC SKIN (SK) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, FOCAL 3148 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1541 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91377 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/06/99 STUDY DAY OF DEATH: 497 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/06/99 13:17 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -INFLAMMATION, ACUTE,-MODERATELY SINGLE MASS, 110 X 80 X 60 MM, RED, SEVERE, FOCAL DARK RED, TAN, FIRM, LOBULATED AND -B-FIBROADENOMA,-NEOPLASM PRESENT VASCULAR; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20); OVERLYING SKIN HAS A SINGLE DARK RED AND BROWN CRUSTED AREA, 70 X 60 MM; COLLECTED INTACT WITH MASS CAUSE OF DEATH (CD) : -MAMMARY FIBROADENOMA,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -DISCHARGE-NASAL; BILATERAL: CLEAR -------------------------------------------- 3149 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1542 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91377 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/06/99 STUDY DAY OF DEATH: 497 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/06/99 13:17 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3150 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1543 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91378 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 285.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:38 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL, MULTI-FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : KIDNEY (KD) : -CYST(S); LEFT, CORTICAL SURFACE: -CYST,-MODERATELY SEVERE, FOCAL MULTIPLE CLEAR FLUID FILLED CYSTS, UP -MINERALIZATION, PELVIC,-MINIMAL, MULTI- TO 8 MM IN DIAMETER FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR -CYST,-PRESENT FLUID FILLED CYST ON EACH, EACH IS 2 MM IN DIAMETER THYMUS (TH) : -M-THYMIC CARCINOMA,-NEOPLASM PRESENT 3151 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1544 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91378 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 285.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:38 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 7 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: -DILATATION,-MODERATE, DIFFUSE TAN FLUID CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3152 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1545 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91378 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 285.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:38 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3153 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1546 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91379 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 229.9 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:00 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0324 .0141 % .0183 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0285 .0124 % .0161 WEIGHT TAKEN BRAIN (BR) 1.7676 .7689 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) .8778 .3818 % .4966 WEIGHT TAKEN RIGHT KIDNEY (KD1) .9029 .3927 % .5108 WEIGHT TAKEN LIVER (LI) 8.1754 3.5561 % 4.6251 WEIGHT TAKEN LUNG (LU) 1.0785 .4691 % .6101 WEIGHT TAKEN LEFT OVARY (OV0) .0520 .0226 % .0294 WEIGHT TAKEN RIGHT OVARY (OV1) .0522 .0227 % .0295 WEIGHT TAKEN SPLEEN (SP) .3743 .1628 % .2118 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0076 .0033 % .0043 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0087 .0038 % .0049 WEIGHT TAKEN UTERUS (UT) .5700 .2479 % .3225 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : -CYST,-SLIGHT, FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL MULTIPLE LINEAR AREAS, UP TO 7 X 1 MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN 3154 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1547 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91379 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 229.9 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:00 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3155 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1548 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91380 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 401.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:35 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT EYE (EY) : EYE (EY) : NECROPSY: OBSERVED -RED INTRAOCULAR MATERIAL; RIGHT -HEMORRHAGE,-MODERATE, FOCAL >MASS(ES); AXILLARY REGION-LEFT; WEEK -INFLAMMATION, ACUTE, CILIARY,-MINIMAL, 92; 35 X 30MM; VERIFIED AT NECROPSY: MULTI-FOCAL OBSERVED BRAIN (BR) : BRAIN (BR) : >EYES; DISCOLORED/LIGHT RED; EYE-RIGHT; -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: OBSERVED -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; LIVER (LI) : LIVER (LI) : WEEK 100; 35 X 35MM; VERIFIED AT -LARGE; ALL LOBES: THICKENED -TELANGIECTASIS,-MINIMAL, MULTI-FOCAL NECROPSY: OBSERVED -DIFFUSELY DARK; ALL LOBES: DARK BROWN KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -MASS(ES); INVOLVING ENTIRE LEFT -ANGIECTASIS,-SEVERE, MULTI-FOCAL ADRENAL: SINGLE MASS, 15 X 10 X 10 MM, -ATROPHY, CORTICAL,-MODERATELY SEVERE, RED, DARK RED, TAN, BROWN AND SOFT; DIFFUSE SAME ON CUT SURFACE; REGIONAL LN (SEE -HEMORRHAGE,-SEVERE, MULTI-FOCAL RENAL) LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL 3156 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1549 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91380 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 401.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:35 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 5 X 5 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, GRAY, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE, FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -HYPERPLASIA, GLANDULAR,-MODERATE, SINGLE MASS, 55 X 45 X 30 MM, RED, MULTI-FOCAL DARK RED, TAN, FIRM AND VASCULAR; SAME -MINERALIZATION,-MINIMAL, MULTI-FOCAL ON CUT SURFACE; COLLECTED; REGIONAL LN -B-FIBROADENOMA,- 3 NEOPLASMS PRESENT (SEE AXILLARY) -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 35 X 25 X 15 MM, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; OVERLYING SKIN HAS A SINGLE DARK RED AND CRUSTED AREA, 10 X 5 MM; COLLECTED; REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS, DIFFUSE (CAP 20) -MASS #3; RIGHT PERINEAL REGION: SINGLE MASS, 60 X 40 X 20 MM, RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED 3157 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1550 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91380 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 401.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:35 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE -M-LEIOMYOSARCOMA,-NEOPLASM PRESENT -MASS(ES); SEROSAL SURFACE OF LEFT DISTAL HORN: SINGLE MASS, 18 X 15 X 10 MM, RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 22) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -LARGE; UP TO 5 MM IN DIAMETER (CAP -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 21) LN, RENAL (RL) : LN, RENAL (RL) : -DIFFUSELY RED; BILATERAL: DARK RED -HEMORRHAGE,-SLIGHT, MULTI-FOCAL (CAP 23) LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS UP TO 12 X -HEMORRHAGE,-MODERATE, MULTI-FOCAL 8 X 3 MM -DIFFUSELY RED; BILATERAL: DARK RED (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -BONE MARROW SMEAR TAKEN -------------------------------------------- 3158 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1551 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91380 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 401.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:35 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 3159 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1552 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91393 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 146.9 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:43 PROSECTOR: TODD ACKER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0267 .0182 % .0159 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0249 .0170 % .0149 WEIGHT TAKEN BRAIN (BR) 1.6759 1.1408 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) .7304 .4972 % .4358 WEIGHT TAKEN RIGHT KIDNEY (KD1) .7355 .5007 % .4389 WEIGHT TAKEN LIVER (LI) 10.9693 7.4672 % 6.5453 WEIGHT TAKEN LUNG (LU) .7636 .5198 % .4556 WEIGHT TAKEN LEFT OVARY (OV0) .0443 .0302 % .0264 WEIGHT TAKEN RIGHT OVARY (OV1) .0472 .0321 % .0282 WEIGHT TAKEN SPLEEN (SP) .3482 .2370 % .2078 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0005 .0003 % .0003 EXCLUDE RIGHT THYROID/PR (TT1) .0092 .0063 % .0055 WEIGHT TAKEN UTERUS (UT) .3120 .2124 % .1862 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 3160 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1553 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91393 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 146.9 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:43 PROSECTOR: TODD ACKER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3161 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1554 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91394 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 184.3 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:10 PROSECTOR: WENDY CHERRY RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0331 .0180 % .0175 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0298 .0162 % .0157 WEIGHT TAKEN BRAIN (BR) 1.8957 1.0286 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) .7737 .4198 % .4081 WEIGHT TAKEN RIGHT KIDNEY (KD1) .8100 .4395 % .4273 WEIGHT TAKEN LIVER (LI) 12.7034 6.8928 % 6.7012 WEIGHT TAKEN LUNG (LU) .8192 .4445 % .4321 WEIGHT TAKEN LEFT OVARY (OV0) .0444 .0241 % .0234 WEIGHT TAKEN RIGHT OVARY (OV1) .0462 .0251 % .0244 WEIGHT TAKEN SPLEEN (SP) .3206 .1740 % .1691 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0051 .0028 % .0027 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0054 .0029 % .0028 WEIGHT TAKEN UTERUS (UT) .4876 .2646 % .2572 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE THYMUS (TH) : THYMUS (TH) : -RED FOCUS(I)/AREA(S); RIGHT LOBE: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL MULTIPLE RED PINPOINT FOCI STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL MULTIPLE DARK BROWN AREAS, UP TO 10 X 1 MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3162 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1555 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91394 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 184.3 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:10 PROSECTOR: WENDY CHERRY RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL NECROPSY OUT OF ORDER ANIMAL DIED PRIOR TO EXSANGUINATION -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3163 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1556 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91404 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 175.9 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:52 PROSECTOR: TODD ACKER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0344 .0196 % .0198 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0385 .0219 % .0222 WEIGHT TAKEN BRAIN (BR) 1.7374 .9877 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) .8122 .4617 % .4675 WEIGHT TAKEN RIGHT KIDNEY (KD1) .7923 .4504 % .4560 WEIGHT TAKEN LIVER (LI) 12.2573 6.9683 % 7.0550 WEIGHT TAKEN LUNG (LU) .8984 .5107 % .5171 WEIGHT TAKEN LEFT OVARY (OV0) .0571 .0325 % .0329 WEIGHT TAKEN RIGHT OVARY (OV1) .0647 .0368 % .0372 WEIGHT TAKEN SPLEEN (SP) .3259 .1853 % .1876 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0099 .0056 % .0057 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0057 .0032 % .0033 WEIGHT TAKEN UTERUS (UT) .5221 .2968 % .3005 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LARGE; ALL LOBES -HEMORRHAGE,-MODERATE, MULTI-FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATELY SEVERE, DIFFUSE -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 3164 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1557 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91404 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 175.9 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:52 PROSECTOR: TODD ACKER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3165 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1558 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91415 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 174.7 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:19 PROSECTOR: GREGORY CODDINGTON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0249 .0143 % .0134 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0175 .0100 % .0094 WEIGHT TAKEN BRAIN (BR) 1.8553 1.0620 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) .7904 .4524 % .4260 WEIGHT TAKEN RIGHT KIDNEY (KD1) .7924 .4536 % .4271 WEIGHT TAKEN LIVER (LI) 11.1073 6.3579 % 5.9868 WEIGHT TAKEN LUNG (LU) .8856 .5069 % .4773 WEIGHT TAKEN LEFT OVARY (OV0) .0367 .0210 % .0198 WEIGHT TAKEN RIGHT OVARY (OV1) .0467 .0267 % .0252 WEIGHT TAKEN SPLEEN (SP) .3928 .2248 % .2117 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0065 .0037 % .0035 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0110 .0063 % .0059 WEIGHT TAKEN UTERUS (UT) .3142 .1799 % .1694 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -DIFFUSELY DARK; ALL LOBES: DARK BROWN -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 3166 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1559 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91415 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 174.7 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:19 PROSECTOR: GREGORY CODDINGTON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3167 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1560 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91423 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 187.0 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:52 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 8.2679 4.4213 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3168 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1561 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91423 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 187.0 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:52 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 3169 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1562 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91427 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 198.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:30 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 8.2478 4.1593 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3170 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1563 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91427 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 198.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:30 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 3171 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1564 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91430 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 161.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:06 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 8.6128 5.3264 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3172 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1565 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91430 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 161.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:06 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 3173 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1566 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91438 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 169.8 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:30 PROSECTOR: DONIA MEYER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0329 .0194 % .0171 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0308 .0181 % .0160 WEIGHT TAKEN BRAIN (BR) 1.9238 1.1330 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) .8302 .4889 % .4315 WEIGHT TAKEN RIGHT KIDNEY (KD1) .8670 .5106 % .4507 WEIGHT TAKEN LIVER (LI) 11.6597 6.8667 % 6.0608 WEIGHT TAKEN LUNG (LU) .9038 .5323 % .4698 WEIGHT TAKEN LEFT OVARY (OV0) .0514 .0303 % .0267 WEIGHT TAKEN RIGHT OVARY (OV1) .0589 .0347 % .0306 WEIGHT TAKEN SPLEEN (SP) .4120 .2426 % .2142 WEIGHT TAKEN LEFT THYROID/PAR (TT0) .0087 .0051 % .0045 WEIGHT TAKEN RIGHT THYROID/PR (TT1) .0076 .0045 % .0040 WEIGHT TAKEN UTERUS (UT) .7756 .4568 % .4032 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LARGE; ALL LOBES -HYPERTROPHY, CENTRILOBULAR, -MOTTLED; ALL LOBES: DARK BROWN AND HEPATOCELLULAR,-MODERATE, DIFFUSE TAN -DIFFUSELY DARK; ALL LOBES: DARK BROWN UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CLEAR FLUID CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN 3174 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1567 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91438 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 169.8 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:30 PROSECTOR: DONIA MEYER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3175 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1568 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91443 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 190.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:30 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 11.7999 6.1877 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3176 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1569 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91443 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 190.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:30 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 3177 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1570 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91448 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 170.2 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:36 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 9.2407 5.4293 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT THYROID/PAR (TT0) -------- -------- -------- NOT TAKEN RIGHT THYROID/PR (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; CAUSE OF DEATH (CD) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3178 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1571 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91448 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 170.2 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:36 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) ------------------------------------------------------------------------------------------------------------------------------------ 3179 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1572 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91451 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 446.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:09 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 92; 30 X 30MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE OBSERVED LIVER (LI) : LIVER (LI) : >MASS(ES); INGUINAL REGION-LEFT; WEEK -LIGHT FOCUS(I)/AREA(S); LEFT LATERAL -DEGENERATION, CYSTIC,-SLIGHT, MULTI- 98; 15 X 20MM; VERIFIED AT NECROPSY: LOBE: MULTIPLE TAN AREAS, UP TO 4 X 3 FOCAL OBSERVED MM; COLLECTED ON ROUTINE SECTION -HEMORRHAGE,-MODERATE, MULTI-FOCAL -MASS #2; RIGHT LATERAL LOBE: SINGLE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- MASS, 15 X 10 X 7 MM, TAN, RED, BROWN FOCAL AND SOFT; SAME ON CUT SURFACE (CAP -NECROSIS, HEPATOCELLULAR,-MODERATE, 20); REGIONAL LN (HEPATIC) NOT MULTI-FOCAL IDENTIFIED -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, MACROPHAGE, ALVEOLAR,- MULTIPLE TAN AREAS, UP TO 10 X 7 MM MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 9 X 6 MM, RED, DARK -CYST,-PRESENT RED, TAN, GRAY AND SOFT -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE >UNREMARKABLE TAN ERODED AREAS, UP TO 3 X 2 MM 3180 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1573 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91451 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 446.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:09 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 25 MM IN DIAMETER, TAN, -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, AND FIRM; SAME ON CUT SURFACE; -PIGMENT,-SLIGHT, MULTI-FOCAL COLLECTED; REGIONAL LN (SEE AXILLARY) -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -CYST(S); ALL REGIONS, DIFFUSE: -M-CARCINOMA,-NEOPLASM PRESENT MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -MASS #2; MID INGUINAL REGION: SINGLE MASS, 35 X 20 X 15 MM, RED, DARK RED, TAN AND LOBULATED; ON SECTION EXUDES A BROWN FLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP >UNREMARKABLE 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3181 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1574 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91451 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 446.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:09 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), PANCREAS (PA), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3182 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1575 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91452 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 564.8 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:31 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 43; 80 X 70MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED KIDNEY (KD) : >APPEARANCE; THIN; VERIFIED AT -MINERALIZATION, PELVIC,-MODERATE, NECROPSY: OBSERVED MULTI-FOCAL >SKIN & PELAGE; SORE/SCAB; VENTRAL- -PYELONEPHRITIS,-SLIGHT, MULTI-FOCAL ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: SPLEEN (SP) : NOT OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL LUNG (LU) : LUNG (LU) : -MASS(ES); RIGHT MIDDLE LOBE: SINGLE -EDEMA, ALVEOLAR,-MODERATELY SEVERE, MASS, 27 X 16 X 10 MM, RED, DARK RED, MULTI-FOCAL TAN, FIRM AND SMOOTH; SAME ON CUT -HEMORRHAGE,-MODERATE, MULTI-FOCAL SURFACE; COLLECTED INTACT; REGIONAL LN -MINERALIZATION,-SLIGHT, FOCAL (MEDIASTINAL) NORMAL (CAP 21) -N-MAMMARY CARCINOMA,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 5 X 4 X 4 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, DARK RED, TAN, SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -EDEMA,-MODERATE, MULTI-FOCAL -GALACTOCELE,-SEVERE, MULTI-FOCAL 3183 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1576 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91452 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 564.8 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:31 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT CERVICAL REGION: -HEMORRHAGE,-MODERATELY SEVERE, MULTI- SINGLE MASS, 95 X 72 X 41 MM (121.4 FOCAL GRAMS), SOME AREAS ARE SOFT AND SOME -B-ADENOMA,-NEOPLASM PRESENT ARE FIRM, RED, DARK RED, TAN AND BROWN; SAME ON CUT SURFACE; ON SECTION, SOME AREAS EXUDE A DARK BROWN FLUID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 20) CERVIX (CV) : >TISSUE MISSING CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 3184 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1577 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91452 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 564.8 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:31 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3185 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1578 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91453 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 315.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 10:08 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -MOTTLED; ALL LOBES: RED, BROWN, AND >UNREMARKABLE >APPEARANCE; THIN; VERIFIED AT TAN NECROPSY: OBSERVED KIDNEY (KD) : KIDNEY (KD) : >DISCHARGE; NASAL; RED IN COLOR; -MASS(ES); BILATERAL, CORTICAL -BACTERIAL COLONIES,-PRESENT VERIFIED AT NECROPSY: OBSERVED SURFACE, EXTENDING INTO MEDULLA: -PYELONEPHRITIS,-MODERATELY SEVERE, SINGLE MASS ON EACH; LEFT IS 20 X 15 X MULTI-FOCAL 8 MM, RIGHT IS 15 X 13 X 5 MM; BOTH ARE RED, TAN, SOFT, AND LOBULATED; ON SECTION OF EACH MASS, SOME AREAS EXUDED A GREEN SEMIFLUID; BOTH MASSES COLLECTED INTACT; REGIONAL LN (SEE RENAL) ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -MASS(ES); RIGHT, INVOLVING ENTIRE -ATROPHY, CORTICAL,-MODERATELY SEVERE, ORGAN: 20 MM IN DIAMETER, RED, DARK DIFFUSE RED, TAN, AND SOFT; SAME ON CUT -HEMORRHAGE,-MODERATE, MULTI-FOCAL SURFACE; COLLECTED; REGIONAL LN (SEE -MINERALIZATION,-MODERATELY SEVERE, RENAL) MULTI-FOCAL -SMALL; LEFT: 4 X 2 X 2 MM -NECROSIS, CORTICAL,-MODERATELY SEVERE, MULTI-FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT ADRENAL, MEDULLA (MA) : -ONE EXAMINED,-PRESENT 3186 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1579 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91453 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 315.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 10:08 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -GELATINOUS; DIFFUSE: CLEAR >TISSUE MISSING OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 2 MM IN DIAMETER LUNG (LU) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -HYPERPLASIA, TYPE II PNEUMOCYTE,- MINIMAL, FOCAL -MINERALIZATION,-MODERATELY SEVERE, MULTI-FOCAL -NECROSIS,-MODERATELY SEVERE, MULTI- FOCAL -N-ADRENAL CORTICAL CARCINOMA,-NEOPLASM PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT, FOCAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT POSTERIOR -B-ADENOMA,-NEOPLASM PRESENT ASPECT: SINGLE RED AREA, 1 MM IN DIAMETER MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,- SLIGHT, DIFFUSE -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL 3187 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1580 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91453 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 315.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 10:08 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE, -NECROSIS, FAT,-PRESENT NEAR RIGHT UTERINE HORN: SINGLE MASS, 5 X 4 X 2 MM, YELLOW AND FIRM (CAP 21); REGIONAL LN (LUMBAR) NORMAL (CAP 22) LN, LUMBAR/ILIAC (LM) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LN, RENAL (RL) : LN, RENAL (RL) : -DIFFUSELY RED; BILATERAL: (CAP 20) -HEMORRHAGE,-MODERATE, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); BILATERAL HIND FEET: -EROSION,-MODERATELY SEVERE, MULTI- SINGLE TAN CRUSTED AREA ON EACH, LEFT FOCAL IS 4 MM IN DIAMETER, RIGHT IS 9 MM IN -FIBROSIS,-MODERATE, MULTI-FOCAL DIAMETER, BOTH COLLECTED -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; BILATERAL: RED -ANIMAL THIN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3188 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1581 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91453 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 315.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 10:08 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA) ------------------------------------------------------------------------------------------------------------------------------------ 3189 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1582 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91454 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/26/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 220.1 GRAMS DATE AND TIME OF NECROPSY: 08/26/99 10:16 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NO FECES; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -DARK FOCUS(I)/AREA(S); DIAPHRAGMATIC -HEMORRHAGE,-MODERATE, FOCAL >SKIN & PELAGE; RED HAIR COAT; HEAD- SURFACE OF LEFT LATERAL LOBE: SINGLE CRANIAL; VERIFIED AT NECROPSY: DARK BROWN AREA, 2 MM IN DIAMETER OBSERVED KIDNEY (KD) : >SKIN & PELAGE; RED HAIR COAT; DORSAL- -CAST, PROTEINACEOUS,-MINIMAL, MULTI- CERVICAL; VERIFIED AT NECROPSY: NOT FOCAL OBSERVED LUNG (LU) : LUNG (LU) : >RESPIRATION; LABORED; VERIFIED AT -LIGHT FOCUS(I)/AREA(S); RIGHT CAUDAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- NECROPSY: NOT APPLICABLE AND LEFT LOBES, NEAR MARGIN: MULTIPLE MINIMAL, MULTI-FOCAL >RESPIRATION; AUDIBLE; VERIFIED AT TAN AREAS, UP TO 3 X 2 MM NECROPSY: NOT APPLICABLE PITUITARY (PI) : >SKIN & PELAGE; BROWN HAIR COAT; -B-ADENOMA,-NEOPLASM PRESENT PERINEAL AREA; VERIFIED AT NECROPSY: STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : OBSERVED -EROSION/ULCERATION; MUCOSA NEAR -EROSION,-MINIMAL, FOCAL JUNCTION OF GLANDULAR MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 1 MM IN DIAMETER CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL 3190 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1583 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91454 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/26/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 220.1 GRAMS DATE AND TIME OF NECROPSY: 08/26/99 10:16 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MEDIASTINAL (ML) : >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST BROWN MATERIAL -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD REGION, DIFFUSE: DARK RED AND DRY MATERIAL -GI TRACT-LARGE LUMEN; ENTIRE GI TRACT: UP TO 15 MM IN DIAMETER; FILLED WITH GAS -------------------------------------------- 3191 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1584 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91454 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/26/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 220.1 GRAMS DATE AND TIME OF NECROPSY: 08/26/99 10:16 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3192 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1585 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91455 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 421.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 12:44 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -RED FOCUS(I)/AREA(S); RIGHT, WITHIN -ANGIECTASIS,-MODERATE, MULTI-FOCAL CORTEX: SINGLE DARK RED, RAISED AREA, -DEGENERATION, CYSTIC, CORTICAL,- 3 MM IN DIAMETER MODERATELY SEVERE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -THROMBUS,-SLIGHT, FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR -CYST,-PRESENT FLUID FILLED CYST ON EACH, LEFT IS 10 -ONE EXAMINED,-PRESENT MM IN DIAMETER, RIGHT IS 7 MM IN DIAMETER PITUITARY (PI) : PITUITARY (PI) : -LARGE; 5 X 4 X 3 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -LIGHT FOCUS(I)/AREA(S); MUCOSA, NEAR >UNREMARKABLE PYLORIC SPHINCTER: TWO TAN AND RAISED AREAS, EACH IS 1 MM IN DIAMETER MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MINIMAL, MULTI-FOCAL 3193 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1586 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91455 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 421.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 12:44 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : -CYST(S); BILATERAL, WITHIN LUMEN: MULTIPLE CLEAR AND RED FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN (CAP -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3194 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1587 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91455 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 421.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 12:44 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LUNG (LU), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3195 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1588 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91456 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 342.7 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:10 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NO FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: OBSERVED LIVER (LI) : >SKIN & PELAGE; YELLOW HAIR COAT; -VACUOLATION, HEPATOCELLULAR,-MODERATE, VERIFIED AT NECROPSY: NOT OBSERVED FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -DARK FOCUS(I)/AREA(S); RIGHT: -DEGENERATION, CYSTIC, CORTICAL,- MULTIPLE DARK BROWN AREAS, UP TO 3 MM MODERATELY SEVERE, FOCAL IN DIAMETER -HEMORRHAGE,-MODERATE, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 9 X 8 MM, RED, DARK -HEMORRHAGE,-MODERATELY SEVERE, MULTI- RED, TAN AND FIRM FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE -EDEMA,-MODERATE, FOCAL -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE -EROSION,-MODERATE, FOCAL DARK BROWN FOCUS, 1 MM IN DIAMETER MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS DIFFUSE: -GALACTOCELE,-MODERATELY SEVERE, MULTI- MULTIPLE WHITE CREAMY SEMIFLUID FILLED FOCAL CYSTS, 5 X 3 X 2 MM (CAP 20) -MINERALIZATION,-MINIMAL, MULTI-FOCAL 3196 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1589 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91456 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 342.7 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:10 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY MATERIAL -------------------------------------------- 3197 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1590 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91456 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 342.7 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:10 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3198 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1591 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91457 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 513.2 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 13:46 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 84; 15 X 15MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC -TELANGIECTASIS,-MINIMAL, FOCAL >RESPIRATION; LABORED; VERIFIED AT SURFACE OF RIGHT MEDIAL LOBE: MULTIPLE NECROPSY: NOT APPLICABLE DARK RED AREAS, UP TO 4 MM IN >DISCHARGE; NASAL; RED IN COLOR; DIAMETER; COLLECTED ON ROUTINE SECTION VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 7 X 5 X 5 MM (LEFT IS 5 -ANGIECTASIS,-MODERATELY SEVERE, MULTI- X 4 X 3 MM) FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MODERATE, MULTI-FOCAL -THROMBUS,-MODERATE, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 6 X 6 MM, RED, TAN AND -B-ADENOMA,-NEOPLASM PRESENT SOFT MAMMARY, FEMALE (MF) : -B-ADENOMA,-NEOPLASM PRESENT 3199 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1592 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91457 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 513.2 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 13:46 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT PERINEAL REGION: SINGLE MASS, 10 MM IN DIAMETER, DARK RED, TAN, BROWN AND SOFT; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : UTERUS (UT) : -CYST(S); WITHIN LUMEN OF MID RIGHT -DILATATION,-MODERATELY SEVERE, FOCAL HORN: SINGLE CLEAR FLUID FILLED CYST, 12 X 6 X 6 MM CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; RED AND MOIST -------------------------------------------- 3200 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1593 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91457 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 513.2 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 13:46 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3201 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1594 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91458 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/05/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 340.2 GRAMS DATE AND TIME OF NECROPSY: 04/05/99 14:36 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL; WEEK 62; SPLEEN (SP) : VERIFIED AT NECROPSY: OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY, >APPEARANCE; PROLAPSED; VAGINA; INCREASED,-MINIMAL, MULTI-FOCAL VERIFIED AT NECROPSY: OBSERVED ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LUNG (LU) : -HEMORRHAGE,-MINIMAL, FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, FOCAL PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); MID ASPECT: -B-ADENOMA,-NEOPLASM PRESENT SINGLE BLACK AREA, 3 X 1 MM -LARGE; 5 X 4 X 3 MM; APPEARS SWOLLEN CERVIX (CV) : -B-POLYP, ENDOMETRIAL STROMAL,-NEOPLASM PRESENT VAGINA (VA) : >UNREMARKABLE >NOTE:>AN ENDOMETRIAL STROMAL POLYP WAS PRESENT IN THE CERVIX 3202 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1595 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91458 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/05/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 340.2 GRAMS DATE AND TIME OF NECROPSY: 04/05/99 14:36 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ VAGINA (VA) : -MASS(ES); WITHIN LUMEN, EXTENDING FROM CERVIX THROUGH VULVA: SINGLE MASS, 38 X 28 X 20 MM, RED, DARK RED, GRAY, TAN, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 20); POSSIBLY CONSISTENT WITH PROLAPSE (VAGINA) AS NOTED IN CLINICAL OBSERVATIONS CAUSE OF DEATH (CD) : -ENDOMETRIAL STROMAL POLYP,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3203 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1596 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91458 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/05/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 340.2 GRAMS DATE AND TIME OF NECROPSY: 04/05/99 14:36 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 3204 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1597 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91459 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 550.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:09 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); VENTRAL-ABDOMINAL; WEEK 102; LIVER (LI) : LIVER (LI) : 20 X 15MM; VERIFIED AT NECROPSY: -LIGHT FOCUS(I)/AREA(S); RIGHT MEDIAN -LIPIDOSIS, SUBCAPSULAR (TENSION OBSERVED LOBE, NEAR JUNCTION WITH LEFT MEDIAN LIPIDOSIS),-MODERATE, FOCAL LOBE: SINGLE TAN AREA, 4 MM IN -TELANGIECTASIS,-MINIMAL, FOCAL DIAMETER; COLLECTED ON ROUTINE SECTION -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE DARK RED AREA, 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 14 MM IN DIAMETER THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, MULTI-FOCAL -HYPERPLASIA, FOLLICULAR CELL,- MODERATE, FOCAL 3205 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1598 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91459 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 550.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:09 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 6 X 3 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; NEAR JUNCTION WITH >UNREMARKABLE NONGLANDULAR STOMACH MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 30 X 25 X 15 MM, RED, -B-FIBROADENOMA,-NEOPLASM PRESENT TAN, FIRM AND LOBULATED; SAME ON CUT -M-CARCINOMA,-NEOPLASM PRESENT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP T0 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -MASS #2; LEFT AXILLARY REGION: TWO MASSES, EACH ARE 10 MM IN DIAMETER, BOTH ARE TAN AND FIRM; SAME ON CUT SURFACES; BOTH COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CERVIX (CV) : CERVIX (CV) : -MASS(ES); ATTACHED TO VENTRAL SEROSAL -M-LEIOMYOSARCOMA,-NEOPLASM PRESENT SURFACE: SINGLE MASS, 6 MM IN DIAMETER, TAN AND FIRM; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 23) 3206 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1599 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91459 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 550.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:09 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ VAGINA (VA) : VAGINA (VA) : -MASS(ES); SEROSAL SURFACE, NEAR -I-LEIOMYOSARCOMA,-NEOPLASM PRESENT BLADDER: SINGLE MASS, 7 X 5 X 3 MM, TAN, FIRM AND LOBULATED; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 23) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -BONE MARROW SMEAR TAKEN -------------------------------------------- 3207 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1600 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91459 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 550.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:09 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), URINARY BLADDER (UB), LN, LUMBAR/ILIAC (LM), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 3208 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1601 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91460 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/30/99 STUDY DAY OF DEATH: 429 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/30/99 14:23 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -DIFFUSELY DARK; ALL LOBES: BROWN -PIGMENT, HEPATOCELLULAR,-MODERATE, >MASS(ES); VENTRAL-ABDOMINAL; WEEK 59; MULTI-FOCAL 40 X 40MM; VERIFIED AT NECROPSY: KIDNEY (KD) : KIDNEY (KD) : OBSERVED -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT >SKIN & PELAGE; PALE; ENTIRE BODY; -MINERALIZATION, PELVIC,-MINIMAL, MULTI- VERIFIED AT NECROPSY: NOT APPLICABLE FOCAL >APPEARANCE; SWOLLEN; VENTRAL- SPLEEN (SP) : SPLEEN (SP) : ABDOMINAL; VERIFIED AT NECROPSY: -LARGE; 62 X 11 X 8 MM -HEMATOPOIESIS, EXTRAMEDULLARY, OBSERVED INCREASED,-MODERATELY SEVERE, MULTI- >EXCRETION; NONFORMED FECES; VERIFIED FOCAL AT NECROPSY: NOT APPLICABLE ADRENAL, CORTEX (AC) : -HEMATOPOIESIS, EXTRAMEDULLARY,- MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -HYPERPLASIA,-MODERATE, FOCAL JEJUNUM (JE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL 3209 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1602 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91460 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/30/99 STUDY DAY OF DEATH: 429 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/30/99 14:23 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CERVIX (CV) : CERVIX (CV) : -MASS(ES); SINGLE MASS, 75 X 50 X 45 -HEMORRHAGE,-MODERATE, MULTI-FOCAL MM (116.0 GRAMS), RED, DARK RED, TAN, -INFLAMMATION, ACUTE,-MODERATE, MULTI- BROWN, FIRM AND LOBULATED; ON SECTION FOCAL APPEARS FIBROUS; COLLECTED; POSSIBLY -B-POLYP, ENDOMETRIAL STROMAL,-NEOPLASM CONSISTENT WITH MASS AS NOTED IN PRESENT CLINICAL OBSERVATIONS; REGIONAL LN (SEE LUMBAR) VAGINA (VA) : >AUTOLYTIC CAUSE OF DEATH (CD) : -ENDOMETRIAL STROMAL POLYP,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL LUMBAR: EACH IS 8 X -HYPERPLASIA, LYMPHOCYTIC,-MINIMAL, 3 X 2 MM (CAP 20) MULTI-FOCAL GENERAL COMMENT (GC) : -DISCHARGE-NASAL; DRY RED MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DARK BROWN AND DRY MATERIAL -ANIMAL THIN -------------------------------------------- 3210 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1603 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91460 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/30/99 STUDY DAY OF DEATH: 429 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/30/99 14:23 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3211 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1604 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91461 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 345.4 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 15:09 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 51; 10 X 10MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; SORE/SCAB; DORSAL; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; FOCAL WEEK 84; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-SLIGHT, MULTI- OBSERVED FOCAL >SKIN & PELAGE; YELLOW HAIR COAT; -PYELONEPHRITIS,-MINIMAL, FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE ADRENAL, CORTEX (AC) : >APPEARANCE; HEAD TILT; LEFT; VERIFIED -ANGIECTASIS,-MINIMAL, MULTI-FOCAL AT NECROPSY: NOT APPLICABLE -DEGENERATION, CYSTIC, CORTICAL,- >APPEARANCE; THIN; VERIFIED AT MINIMAL, FOCAL NECROPSY: OBSERVED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 10 MM IN DIAMETER THYMUS (TH) : -CYST,-PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL TAN, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT 3212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1605 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91461 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 345.4 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 15:09 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); DORSAL THORACIC -ATROPHY, ADNEXAL,-MODERATE, MULTI- REGION: MULTIPLE DARK RED CRUSTED FOCAL AREAS, UP TO 3 MM IN DIAMETER; -INFLAMMATION, CHRONIC-ACTIVE,- COLLECTED WITH ALOPECIA MODERATE, MULTI-FOCAL -ALOPECIA-DIFFUSE; ENTIRE DORSAL -ULCERATION,-MODERATE, FOCAL THORACIC REGION (CAP 22) MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT VENTRAL ABDOMINAL -GALACTOCELE,-SEVERE, MULTI-FOCAL REGION: SINGLE MASS, 31 X 25 X 9 MM, -INFLAMMATION, CHRONIC-ACTIVE,- TAN, GRAY, DARK RED, LOBULATED AND MODERATELY SEVERE, FOCAL CYSTIC; SOME SECTIONS EXUDE A TAN AND -B-ADENOMA,-NEOPLASM PRESENT CREAMY SEMIFLUID; COLLECTED; REGIONAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT LN (SEE INGUINAL) -MASS #2; RIGHT VENTRAL ABDOMINAL REGION: SINGLE MASS, 29 X 23 X 14 MM, TAN, BROWN, LOBULATED AND FIRM; SOME SECTIONS EXUDE A TAN AND CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (SEE INGUINAL) CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, INGUINAL (IN) : LN, INGUINAL (IN) : -LARGE; LEFT: 11 X 6 X 6 MM (RIGHT NOT >TISSUE MISSING IDENTIFIED) (CAP 21) >NOTE:>INGUINAL LYMPH NODE DESCRIBED GROSSLY WAS A MAMMARY ADENOMA GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN 3213 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1606 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91461 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 345.4 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 15:09 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3214 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1607 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91462 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 481.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:08 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-SLIGHT, FOCAL LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); ALL REGIONS, -B-ADENOMA,-NEOPLASM PRESENT DIFFUSE: MULTIPLE RED AREAS, UP TO 2 X 1 MM -LARGE; 6 X 6 X 4 MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3215 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1608 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91462 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 481.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:08 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), MAMMARY, FEMALE (MF), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3216 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1609 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91463 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/25/99 STUDY DAY OF DEATH: 608 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 363.3 GRAMS DATE AND TIME OF NECROPSY: 09/25/99 13:17 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; BRAIN (BR) : BRAIN (BR) : WEEK 66; 25 X 30MM; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); AXILLARY REGION-LEFT; WEEK KIDNEY (KD) : 71; 20 X 20MM; VERIFIED AT NECROPSY: -CAST, PROTEINACEOUS,-SLIGHT, MULTI- OBSERVED FOCAL >EYES; RED DISCHARGE; EYES; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: OBSERVED -ANGIECTASIS,-MODERATE, MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -HEMORRHAGE,-MODERATE, MULTI-FOCAL NECROPSY: NOT APPLICABLE PITUITARY (PI) : PITUITARY (PI) : >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED -MASS(ES); SINGLE MASS, 7 X 7 X 9 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL AT NECROPSY: NOT APPLICABLE FIRM, RED, DARK RED AND TAN -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -CYST,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 13 X 23 X 37 MM, FIRM, -M-CARCINOMA,-NEOPLASM PRESENT RED, TAN AND LOBULATED; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (AXILLARY) NORMAL (CAP 22) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 23 X 34 X 37 MM, FIRM, RED, TAN AND LOBULATED; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (INGUINAL) NOT IDENTIFIED 3217 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1610 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91463 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/25/99 STUDY DAY OF DEATH: 608 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 363.3 GRAMS DATE AND TIME OF NECROPSY: 09/25/99 13:17 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -------------------------------------------- 3218 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1611 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91463 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/25/99 STUDY DAY OF DEATH: 608 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 363.3 GRAMS DATE AND TIME OF NECROPSY: 09/25/99 13:17 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 3219 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1612 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91464 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 304.8 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:31 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL; WEEK 35; LIVER (LI) : 40 X 40MM; VERIFIED AT NECROPSY: -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- OBSERVED FOCAL >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -INFARCT,-SLIGHT, MULTI-FOCAL >SKIN & PELAGE; SORE/SCAB; VENTRAL- -MINERALIZATION, TUBULAR,-SLIGHT, MULTI- ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: FOCAL OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, FOCAL LUNG (LU) : -MINERALIZATION,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 MM IN DIAMETER; APPEARS -B-ADENOMA,-NEOPLASM PRESENT SWOLLEN SKIN (SK) : -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-SLIGHT, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT 3220 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1613 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91464 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 304.8 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:31 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION, EXTENDING TO MID PERIANAL REGION: SINGLE MASS, 46 X 37 X 24 MM, TAN, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; OVERLYING SKIN HAS A SINGLE MOIST RED ULCERATED AREA, 14 X 11 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT AORTA (AO) : AORTA (AO) : -FIRM; DIFFUSE -MINERALIZATION,-MODERATELY SEVERE, DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 3221 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1614 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91464 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 304.8 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:31 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3222 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1615 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91465 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/11/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 221.9 GRAMS DATE AND TIME OF NECROPSY: 10/11/99 14:07 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; YELLOW HAIR COAT; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >APPEARANCE; THIN; VERIFIED AT SLIGHT, MULTI-FOCAL NECROPSY: OBSERVED ADRENAL, CORTEX (AC) : >APPEARANCE; DEHYDRATED; VERIFIED AT -ANGIECTASIS,-MODERATELY SEVERE, MULTI- NECROPSY: NOT APPLICABLE FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL, MULTI-FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, RED, DARK -HEMORRHAGE,-SLIGHT, MULTI-FOCAL RED, TAN, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -GALACTOCELE,-MODERATE, MULTI-FOCAL AND FIRM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 3223 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1616 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91465 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/11/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 221.9 GRAMS DATE AND TIME OF NECROPSY: 10/11/99 14:07 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; PERINEUM: YELLOW AND MOIST -BLOOD FILM TAKEN -MALOCCLUSION; LEFT UPPER INCISOR -ANIMAL THIN -TEETH MISSING/BROKEN/WORN; RIGHT UPPER INCISOR: BROKEN OFF AT BASE -------------------------------------------- 3224 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1617 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91465 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/11/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 221.9 GRAMS DATE AND TIME OF NECROPSY: 10/11/99 14:07 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3225 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1618 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91466 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 451.7 GRAMS DATE AND TIME OF NECROPSY: 11/03/99 13:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : LEFT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; RED HAIR COAT; HEAD- -DILATATION, VENTRICULAR,-PRESENT ENTIRE; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, NECROPSY: OBSERVED EOSINOPHILIC,-SEVERE, FOCAL >EXCRETION; NO FECES; VERIFIED AT -HEMORRHAGE,-MODERATE, MULTI-FOCAL NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR,- >BEHAVIOR; HYPOACTIVE; VERIFIED AT MODERATELY SEVERE, MULTI-FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : KIDNEY (KD) : >SKIN & PELAGE; YELLOW HAIR COAT; -GRANULAR MATERIAL; BILATERAL, WITHIN -CAST, PROTEINACEOUS,-MINIMAL, MULTI- VERIFIED AT NECROPSY: NOT OBSERVED PELVIS: MULTIPLE TAN, FIRM GRANULES, FOCAL >DISCHARGE; NASAL; RED IN COLOR; PINPOINT AND UP TO 1 MM IN DIAMETER -MINERALIZATION, PELVIC,-MODERATE, VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); LEFT, AT MULTI-FOCAL CRANIAL END: SINGLE TAN, SLIGHTLY -B-LIPOMA,-NEOPLASM PRESENT RAISED AREA, 6 X 5 X 4 MM ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 4 MM IN DIAMETER 3226 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1619 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91466 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 451.7 GRAMS DATE AND TIME OF NECROPSY: 11/03/99 13:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ BONE, FEMUR (FE) : BONE, FEMUR (FE) : >NOTE:>TRIMMING COMMENT: MISSING AT >TISSUE MISSING TIME OF TRIMMING MARROW, FEMUR (FM) : MARROW, FEMUR (FM) : >NOTE:>TRIMMING COMMENT: MISSING AT >TISSUE MISSING TIME OF TRIMMING LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 7 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- GREY AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -EDEMA,-MODERATE, FOCAL TAN ERODED AREAS, UP TO 3 X 2 MM -INFLAMMATION, ACUTE,-MINIMAL, MULTI- FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE GREEN MUCOID SEMIFLUID MATERIAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: -FIBROSIS,-MODERATELY SEVERE, FOCAL SINGLE DRY, BROWN CRUSTED AREA, 9 X 7 -INFLAMMATION, ACUTE,-MODERATE, FOCAL MM; COLLECTED 3227 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1620 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91466 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 451.7 GRAMS DATE AND TIME OF NECROPSY: 11/03/99 13:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED, DRY MATERIAL -DISCHARGE-NASAL; DRY, RED MATERIAL -STAINS - SKIN/HAIRCOAT; INVOLVING ENTIRE HEAD: DRY, RED MATERIAL -------------------------------------------- 3228 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1621 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91466 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 451.7 GRAMS DATE AND TIME OF NECROPSY: 11/03/99 13:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3229 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1622 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91467 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 467.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:40 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SCALY SKIN; TAIL; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; SORE/SCAB; LIMBS-HIND; -DILATATION, VENTRICULAR,-PRESENT VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 7 X 5 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL GREY, AND FIRM -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -DILATATION, SINUSOIDAL,-MODERATE, (CAP 20) FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL TAIL (TI) : -EROSION,-SLIGHT, MULTI-FOCAL 3230 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1623 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91467 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 467.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:40 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TAIL (TI) : TAIL (TI) : -CRUSTED AREA(S); ALONG ENTIRE LENGTH: -INFLAMMATION, ACUTE,-SLIGHT, MULTI- MULTIPLE TAN, DRY, CRUSTED AREAS, UP FOCAL TO 1 MM IM DIAMETER; COLLECTED FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); BILATERAL HIND FEET: -EROSION,-MODERATE, FOCAL SINGLE CRUSTED AREA ON EACH; LEFT IS 6 -FIBROSIS,-MODERATE, FOCAL X 5 MM, RED, TAN, AND DRY; RIGHT IS 2 -INFLAMMATION, ACUTE,-SLIGHT, FOCAL MM IN DIAMETER, TAN AND DRY; BOTH COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3231 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1624 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91467 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 467.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:40 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), LUNG (LU), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3232 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1625 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91468 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/12/99 STUDY DAY OF DEATH: 442 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/12/99 11:40 PROSECTOR: STEVE VAN ADESTINE RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LUNG (LU) : LUNG (LU) : -RED FOCUS(I)/AREA(S); ALL LOBES: >UNREMARKABLE MULTIPLE DARK RED AREAS, PINPOINT AND UP TO 6 X 3 MM PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT JEJUNUM (JE) : -CYST, KERATIN,-SEVERE, FOCAL 3233 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1626 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91468 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/12/99 STUDY DAY OF DEATH: 442 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/12/99 11:40 PROSECTOR: STEVE VAN ADESTINE RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ JEJUNUM (JE) : -MASS(ES); MID AND DISTAL JEJUNUM, SEROSAL SURFACE: TWO MASSES, MID IS 10 X 9 X 7 MM, DISTAL IS 15 X 12 X 11 MM, TAN, GRAY AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MESENTERIC) NORMAL UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: -DILATATION,-MODERATELY SEVERE, DIFFUSE LIGHT GREEN, CLOUDY FLUID -LARGE, DIFFUSE; BILATERAL: EACH IS UP TO 20 MM IN DIAMETER CAUSE OF DEATH (CD) : -KERATIN CYST,-PRESENT CAVITY, THORACIC (TA) : CAVITY, THORACIC (TA) : >NOTE:>PERICARDIAL SAC AND AORTIC ARCH >NOT REQUIRED TO BE EXAMINED FOR ANIMAL LACERATED; HEART FOUND FLOATING FREE WITHIN CAVITY GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL UPPER INCISORS -FLUID-THORACIC CAVITY; DARK RED, GELATINOUS, SEMIFLUID -------------------------------------------- 3234 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1627 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91468 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/12/99 STUDY DAY OF DEATH: 442 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/12/99 11:40 PROSECTOR: STEVE VAN ADESTINE RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3235 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1628 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91469 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/09/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/09/99 8:40 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYE-LEFT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; ROUGH HAIR COAT; -HEMORRHAGE,-MODERATE, MULTI-FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >SKIN & PELAGE; RED HAIR COAT; HEAD- -MINERALIZATION, PELVIC,-MINIMAL, MULTI- ENTIRE; VERIFIED AT NECROPSY: NOT FOCAL OBSERVED SPLEEN (SP) : >SKIN & PELAGE; RED HAIR COAT; PERINEAL -PIGMENT, INCREASED,-MINIMAL, MULTI- AREA; VERIFIED AT NECROPSY: NOT FOCAL OBSERVED PANCREAS (PA) : >EXCRETION; FEW FECES; VERIFIED AT -B-ADENOMA, ISLET CELL,-NEOPLASM NECROPSY: NOT APPLICABLE PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SEVERE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- MINIMAL, MULTI-FOCAL -HEMORRHAGE,-SEVERE, MULTI-FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT, MULTI-FOCAL LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, MULTI-FOCAL 3236 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1629 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91469 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/09/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/09/99 8:40 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 11 X 7 X 5 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, DARK RED, TAN, LOBULATED, AND -B-ADENOMA,-NEOPLASM PRESENT SOFT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: RED, DRY MATERIAL -------------------------------------------- 3237 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1630 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91469 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/09/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/09/99 8:40 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3238 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1631 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91470 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 294.4 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 15:45 PROSECTOR: NANCY DIEDRICH RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; PALE; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; COLD TO TOUCH; ENTIRE -DILATATION, VENTRICULAR,-PRESENT BODY; VERIFIED AT NECROPSY: NOT LIVER (LI) : LIVER (LI) : APPLICABLE -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -HEMATOPOIESIS, EXTRAMEDULLARY,- >EXCRETION; NO FECES; VERIFIED AT MULTIPLE TAN AND FIRM FOCI, PINPOINT MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE AND UP TO 1 MM IN DIAMETER -NECROSIS, HEPATOCELLULAR,-MODERATELY >EYES; RED DISCHARGE; EYES; VERIFIED AT -MOTTLED; MARGIN OF LEFT LATERAL LOBE: SEVERE, MULTI-FOCAL NECROPSY: OBSERVED SINGLE AREA, 28 X 13 MM, AREA APPEARS -VACUOLATION, HEPATOCELLULAR,-MINIMAL, >SKIN & PELAGE; RED HAIR COAT; VENTRAL- MOTTLED, RED, TAN AND FIRM; COLLECTED MULTI-FOCAL ABDOMINAL; VERIFIED AT NECROPSY: KIDNEY (KD) : OBSERVED -MINERALIZATION, PELVIC,-MODERATE, >APPEARANCE; THIN; VERIFIED AT MULTI-FOCAL NECROPSY: NOT OBSERVED SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, DIFFUSE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, FOCAL -HEMORRHAGE,-MODERATE, FOCAL OVARY (OV) : -CYST,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, RED, TAN AND -B-ADENOMA,-NEOPLASM PRESENT SOFT 3239 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1632 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91470 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 294.4 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 15:45 PROSECTOR: NANCY DIEDRICH RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -EDEMA,-MODERATELY SEVERE, MULTI-FOCAL TAN ERODED AREAS, UP TO 8 X 5 MM -INFLAMMATION, ACUTE,-MODERATE, MULTI- -THICKENED WALL; MUCOSA, DIFFUSE FOCAL -ULCERATION,-MODERATELY SEVERE, MULTI- FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL UTERUS (UT) : -DILATATION,-SLIGHT, FOCAL VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; TAN AND MUCOID >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DARK BROWN AND DRY -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND CRUSTED -STAINS - SKIN/HAIRCOAT; ABDOMEN AND CHEST: RED AND DRY -GI TRACT-DARK DRY CONTENT; CECUM: DARK BROWN SEMIFLUID -------------------------------------------- 3240 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1633 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91470 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 294.4 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 15:45 PROSECTOR: NANCY DIEDRICH RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3241 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1634 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91471 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 675 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 297.7 GRAMS DATE AND TIME OF NECROPSY: 12/01/99 13:21 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT OBSERVED -MOTTLED; ALL LOBES: RED, DARK RED, -VACUOLATION, HEPATOCELLULAR,-SLIGHT, >EYES; RED DISCHARGE; EYES; VERIFIED AT TAN AND BROWN MULTI-FOCAL NECROPSY: OBSERVED KIDNEY (KD) : KIDNEY (KD) : >EXCRETION; NO FECES; VERIFIED AT -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE -DIFFUSELY LIGHT; BILATERAL: TAN SEVERE, DIFFUSE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 3 MM IN DIAMETER THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); MID ANTERIOR -HEMORRHAGE,-MODERATE, MULTI-FOCAL ASPECT: SINGLE RED AREA, 2 X 1 MM -B-ADENOMA,-NEOPLASM PRESENT SALIV GL, MANDIB (SG) : SALIV GL, MANDIB (SG) : >NOTE:>TRIMMING COMMENT: MANDIBULAR >TISSUE MISSING SALIVARY GLANDS ARE BOTH MISSING MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT 3242 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1635 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91471 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 675 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 297.7 GRAMS DATE AND TIME OF NECROPSY: 12/01/99 13:21 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); LEFT LATERAL CERVICAL REGION: SINGLE MASS, 12 X 10 X 8 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL; COLLECTED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -CHRONIC PROGRESSIVE NEPHROPATHY,- PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED -------------------------------------------- 3243 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1636 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91471 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 675 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 297.7 GRAMS DATE AND TIME OF NECROPSY: 12/01/99 13:21 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3244 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1637 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91472 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 674.9 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 12:21 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK LIVER (LI) : 67; 100 X 115MM; VERIFIED AT NECROPSY: -VACUOLATION, HEPATOCELLULAR,-MINIMAL, OBSERVED MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, MULTI-FOCAL -B-ADENOMA,- 2 NEOPLASMS PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT VENTRAL ABDOMINAL -B-ADENOMA,-NEOPLASM PRESENT REGION: SINGLE MASS, 115 X 105 X 45 MM (300.2 GRAMS), TAN, RED, FIRM, LOBULATED AND VASCULAR; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -MAMMARY FIBROADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3245 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1638 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91472 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 674.9 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 12:21 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3246 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1639 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91473 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 494.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 10:45 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 87; 20 X 20MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); CAUDATE LOBE: -LIPIDOSIS, SUBCAPSULAR (TENSION SINGLE TAN AREA, 2 MM IN DIAMETER; LIPIDOSIS),-MODERATE, FOCAL COLLECTED >NOTE:>MINIMAL FOCAL EOSINOPHILIC FOCUS, MINIMAL FOCAL CYSTIC DEGENERATION, MINIMAL MULTIFOCAL VACUOLATION KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI- FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 7 X 5 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL GRAY AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA, NEAR >UNREMARKABLE JUNCTION WITH NONGLANDULAR MUCOSA: SINGLE RED ERODED AREA, 2 MM IN DIAMETER 3247 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1640 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91473 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 494.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 10:45 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL SINGLE MASS, 20 X 15 X 10 MM, RED, -B-FIBROADENOMA,-NEOPLASM PRESENT TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3248 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1641 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91473 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 494.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 10:45 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), URINARY BLADDER (UB), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 3249 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1642 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91474 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 315.2 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 11:46 PROSECTOR: NANCY DIEDRICH RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT OBSERVED LIVER (LI) : >APPEARANCE; HEAD TILT; LEFT; VERIFIED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- AT NECROPSY: NOT APPLICABLE FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 10 MM IN DIAMETER PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 10 X 8 MM, RED, DARK -HEMORRHAGE,-MODERATELY SEVERE, MULTI- RED, GREY AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT PERINEAL REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 30 X 25 X 15 MM, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE GREEN MUCOID SEMIFLUID MATERIAL 3250 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1643 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91474 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 315.2 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 11:46 PROSECTOR: NANCY DIEDRICH RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT TONGUE (TO) : TONGUE (TO) : -MASS(ES); VENTRAL SURFACE, AT BASE: -CYST,-PRESENT SINGLE MASS, 3 MM IN DIAMETER, TAN AND SOFT; ON SECTION EXUDES A TAN GRANULAR SEMISOLID MATERIAL; COLLECTED INTACT; REGIONAL LN (MANDIBULAR) NORMAL (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND CRUSTED -MALOCCLUSION; BILATERAL, UPPER INCISORS -------------------------------------------- 3251 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1644 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91474 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 315.2 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 11:46 PROSECTOR: NANCY DIEDRICH RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3252 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1645 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91475 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 523.0 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 7:54 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; LIMBS-HIND; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EYES; CLEAR DISCHARGE; EYE-RIGHT; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: NOT OBSERVED -MOTTLED; ALL LOBES: BROWN AND TAN -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >SKIN & PELAGE; RED HAIR COAT; PERINEAL FOCAL AREA; VERIFIED AT NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR,- >EXCRETION; NO FECES; VERIFIED AT MODERATELY SEVERE, MULTI-FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL SPLEEN (SP) : SPLEEN (SP) : -SMALL; 32 X 9 X 6 MM >UNREMARKABLE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 10 X 10 X 9 MM (LEFT IS -ANGIECTASIS,-MODERATE, MULTI-FOCAL 6 X 5 X 4 MM) -DEGENERATION, CYSTIC, CORTICAL,- SEVERE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -THROMBUS,-MODERATELY SEVERE, MULTI- FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 3 MM IN DIAMETER THYMUS (TH) : -CYST,-PRESENT 3253 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1646 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91475 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 523.0 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 7:54 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, MACROPHAGE, ALVEOLAR,- MULTIPLE TAN AREAS, UP TO 2 MM IN SLIGHT, MULTI-FOCAL DIAMETER -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 10 X 9 MM, DARK RED, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL TAN, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE >UNREMARKABLE TAN ERODED AREAS, UP TO 3 MM IN DIAMETER UTERUS (UT) : UTERUS (UT) : -MASS(ES); INVOLVING ENTIRE LEFT HORN: -DILATATION,-SEVERE, DIFFUSE SINGLE MASS, 45 X 17 X 17 MM, RED, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL DARK RED, TAN, AND FIRM; SAME ON CUT -INFLAMMATION, ACUTE,-SLIGHT, MULTI- SURFACE; COLLECTED INTACT; REGIONAL LN FOCAL (SEE LUMBAR) -NECROSIS,-MODERATE, MULTI-FOCAL -B-POLYP, ENDOMETRIAL, STROMAL,- NEOPLASM PRESENT VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE CONTAINS A DARK RED SEMIFLUID CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 3254 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1647 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91475 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 523.0 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 7:54 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE -NECROSIS, FAT,-PRESENT CRANIAL TO RIGHT OVARY AND MEDIAL TO RIGHT UTERINE HORN: TWO MASSES, ONE IS 15 X 10 X 5 MM, THE OTHER IS 7 X 6 X 5 MM, BOTH ARE YELLOW AND FIRM; BOTH ARE SAME ON CUT SURFACE (CAP 22); REGIONAL LN (SEE LUMBAR) LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: EACH IS UP TO 7 X 7 -DILATATION, SINUSOIDAL,-SLIGHT, MULTI- X 5 MM (CAP 23) FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); BILATERAL HIND FEET: -EDEMA,-MODERATE, FOCAL SINGLE DARK RED AND DRY AREA ON EACH, -EROSION,-MODERATE, FOCAL LEFT IS 5 MM IN DIAMETER, RIGHT IS 7 -INFLAMMATION, ACUTE,-MINIMAL, MULTI- MM IN DIAMETER; BOTH COLLECTED FOCAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DARK RED AND MOIST -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DARK RED AND DRY -------------------------------------------- 3255 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1648 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91475 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 523.0 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 7:54 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3256 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1649 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91476 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/05/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 303.6 GRAMS DATE AND TIME OF NECROPSY: 08/05/99 15:15 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; YELLOW HAIR COAT; -DILATATION, VENTRICULAR,-PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE LIVER (LI) : >RESPIRATION; AUDIBLE; VERIFIED AT -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : NECROPSY: NOT APPLICABLE -LARGE; LEFT: 4 X 4 X 6 MM (RIGHT IS 3 -ANGIECTASIS,-SLIGHT, MULTI-FOCAL >RESPIRATION; IRREGULAR; VERIFIED AT X 4 X 5 MM) -HEMORRHAGE,-SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE ADRENAL, MEDULLA (MA) : >EXCRETION; NO FECES; VERIFIED AT -VACUOLATION,-MODERATE, MULTI-FOCAL NECROPSY: NOT APPLICABLE -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 7 X 11 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL SOFT, RED, DARK RED AND TAN -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: -GALACTOCELE,-SLIGHT, MULTI-FOCAL COLLECTED WITH ROUTINE SECTION CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 3257 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1650 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91476 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/05/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 303.6 GRAMS DATE AND TIME OF NECROPSY: 08/05/99 15:15 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3258 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1651 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91477 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 296.1 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:42 PROSECTOR: DENNIS HOFFMAN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 46; 60 X 70MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >EYES; SQUINTED; PERI-ORBITAL; VERIFIED FOCAL AT NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MODERATE, >EYES; RED DISCHARGE; EYES; VERIFIED AT MULTI-FOCAL NECROPSY: OBSERVED ADRENAL, CORTEX (AC) : >SKIN & PELAGE; YELLOW HAIR COAT; -DEGENERATION, CYSTIC, CORTICAL,- VERIFIED AT NECROPSY: OBSERVED MINIMAL, MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT LUNG (LU) : NECROPSY: NOT APPLICABLE -MINERALIZATION,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 6 X 10 MM, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- SOFT, RED, DARK RED AND TAN FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT PERIANAL REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL SINGLE MASS, 33 X 50 X 68 MM, RED, -B-FIBROADENOMA,-NEOPLASM PRESENT DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED VAGINA (VA) : >UNREMARKABLE 3259 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1652 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91477 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 296.1 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:42 PROSECTOR: DENNIS HOFFMAN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN MUCOID MATERIAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 3260 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1653 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91477 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 296.1 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:42 PROSECTOR: DENNIS HOFFMAN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3261 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1654 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91478 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/10/99 STUDY DAY OF DEATH: 593 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/10/99 13:52 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK LIVER (LI) : LIVER (LI) : 78; 70 X 70MM; VERIFIED AT NECROPSY: -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -NECROSIS, HEPATOCELLULAR,-MODERATE, OBSERVED MULTIPLE TAN AREAS, UP TO 3 MM IN MULTI-FOCAL >SKIN & PELAGE; SORE/SCAB; DORSAL- DIAMETER; COLLECTED ON ROUTINE SECTION -THROMBUS,-MODERATE, FOCAL THORACIC-LEFT; VERIFIED AT NECROPSY: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO OBSERVED NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT >SKIN & PELAGE; RED HAIR COAT; HEAD- KIDNEY (KD) : CRANIAL; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-SLIGHT, MULTI- OBSERVED FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE, DIFFUSE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE, FOCAL -THROMBUS,-MODERATELY SEVERE, FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LUNG (LU) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI- FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL 3262 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1655 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91478 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/10/99 STUDY DAY OF DEATH: 593 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/10/99 13:52 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT ILEUM (IL) : >AUTOLYTIC RECTUM (RE) : >TISSUE MISSING SKIN (SK) : -INFLAMMATION, ACUTE,-MODERATE, FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO SINGLE MASS, 70 X 50 X 35 MM, RED, NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE RED AND DARK RED CRUSTED AREA, 55 X 34 MM; COLLECTED INTACT WITH MASS UTERUS (UT) : -DILATATION,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -MALIGNANT FIBROUS HISTIOSARCOMA,- PRESENT HEMATO NEOPLASIA (HN) : -M-MALIGNANT FIBROUS HISTIOCYTOMA,- NEOPLASM PRESENT 3263 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1656 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91478 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/10/99 STUDY DAY OF DEATH: 593 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/10/99 13:52 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; LEFT: 34 X 17 X 8 MM (RIGHT IS -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO 4 X 3 X 2 MM) (CAP 21) NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT GENERAL COMMENT (GC) : -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: RED AND DRY MATERIAL -------------------------------------------- 3264 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1657 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91478 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/10/99 STUDY DAY OF DEATH: 593 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/10/99 13:52 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA) ------------------------------------------------------------------------------------------------------------------------------------ 3265 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1658 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91479 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 596.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:38 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC -FIBROSIS,-SLIGHT, MULTI-FOCAL SURFACE OF LEFT LATERAL LOBE: SINGLE -TELANGIECTASIS,-SLIGHT, MULTI-FOCAL TAN AREA, 15 X 3 MM; COLLECTED ON -VACUOLATION, HEPATOCELLULAR,-SLIGHT, ROUTINE SECTION MULTI-FOCAL -RED FOCUS(I)/AREA(S); ALL LOBES: -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM MULTIPLE DARK RED AREAS, UP TO 6 MM IN PRESENT DIAMETER; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI- FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3266 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1659 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91479 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 596.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:38 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3267 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1660 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91480 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 421.5 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 12:49 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -TELANGIECTASIS,-MINIMAL, FOCAL MULTIPLE DARK RED AREAS, UP TO 5 MM IN DIAMETER; COLLECTED SECTIONS KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 6 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- TAN, GREY, AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL CERVIX (CV) : -B-LEIOMYOMA,-NEOPLASM PRESENT 3268 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1661 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91480 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 421.5 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 12:49 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CERVIX (CV) : -MASS(ES); EXTENDING INTO VAGINA: SINGLE MASS, 12 X 10 X 10 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (ILIAC) NORMAL (CAP 20) UTERUS (UT) : UTERUS (UT) : -CYST(S); MID PORTION OF RIGHT HORN: -DILATATION,-MODERATE, FOCAL SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, OTHER (LN) : >UNREMARKABLE >NOTE:>ILIAC LYMPH NODE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3269 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1662 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91480 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 421.5 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 12:49 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3270 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1663 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91481 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 303.6 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 14:45 PROSECTOR: KEVIN BILLINGS RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 61; 50 X 40MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; SORE/SCAB; VENTRAL- -HEMORRHAGE,-MODERATE, MULTI-FOCAL ABDOMINAL; VERIFIED AT NECROPSY: -I-PITUITARY CARCINOMA,-NEOPLASM OBSERVED PRESENT >SKIN & PELAGE; BLUE SKIN; VENTRAL- KIDNEY (KD) : ABDOMINAL; VERIFIED AT NECROPSY: NOT -MINERALIZATION, PELVIC,-SLIGHT, MULTI- APPLICABLE FOCAL >APPEARANCE; THIN; VERIFIED AT -MINERALIZATION, TUBULAR,-MINIMAL, NECROPSY: NOT APPLICABLE MULTI-FOCAL >APPEARANCE; HUNCHED; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: NOT APPLICABLE -DEGENERATION, CYSTIC, CORTICAL,- >BEHAVIOR; ATAXIC; VERIFIED AT SLIGHT, FOCAL NECROPSY: NOT APPLICABLE OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 6 MM IN DIAMETER THYMUS (TH) : THYMUS (TH) : >NOTE:>TRIMMING COMMENT: MISSING AT >TISSUE MISSING TRIMMING LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 3271 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1664 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91481 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 303.6 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 14:45 PROSECTOR: KEVIN BILLINGS RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, DARK RED -M-CARCINOMA,-NEOPLASM PRESENT AND SOFT STOMACH, NONGL (SU) : -EDEMA,-SLIGHT, MULTI-FOCAL SKIN (SK) : SKIN (SK) : >NOTE:>COLLECTED FROM RIGHT INGUINAL -EDEMA,-MODERATE, FOCAL REGION DUE TO MAMMARY MASS -INFLAMMATION, ACUTE,-MODERATE, FOCAL -ULCERATION,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION, -M-CARCINOMA,-NEOPLASM PRESENT EXTENDING TO LEFT PERIANAL REGION: SINGLE MASS, 70 X 50 X 25 MM, RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN HAS MULTIPLE DARK RED AND DRY ABRADED AREAS, UP TO 8 MM IN DIAMETER; COLLECTED INTACT WITH MASS >NOTE:>COLLECTED FROM RIGHT INGUINAL REGION DUE TO MAMMARY MASS CAUSE OF DEATH (CD) : -PITUITARY CARCINOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3272 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1665 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91481 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 303.6 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 14:45 PROSECTOR: KEVIN BILLINGS RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3273 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1666 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91482 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 281.6 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 11:45 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; BRAIN (BR) : BRAIN (BR) : WEEK 72; 30 X 35MM; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED -DILATATION, VENTRICULAR,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT LIVER (LI) : NECROPSY: NOT OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >APPEARANCE; THIN; VERIFIED AT FOCAL NECROPSY: OBSERVED KIDNEY (KD) : >SKIN & PELAGE; ROUGH HAIR COAT; -MINERALIZATION, PELVIC,-MINIMAL, MULTI- VERIFIED AT NECROPSY: NOT APPLICABLE FOCAL >APPEARANCE; HEAD TILT; RIGHT; VERIFIED ADRENAL, CORTEX (AC) : AT NECROPSY: NOT APPLICABLE -HEMORRHAGE,-MINIMAL, MULTI-FOCAL >APPEARANCE; HUNCHED; VERIFIED AT LN, MESENTERIC (MS) : NECROPSY: NOT APPLICABLE -INFILTRATE, MACROPHAGE, PIGMENTED,- >EYES; PALE; EYES; VERIFIED AT MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE THYMUS (TH) : >BEHAVIOR; ATAXIC; VERIFIED AT -CYST,-PRESENT NECROPSY: NOT APPLICABLE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 6 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL SKIN (SK) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE, FOCAL 3274 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1667 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91482 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 281.6 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 11:45 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : -ULCERATION,-SLIGHT, FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: TWO -GALACTOCELE,-MODERATE, MULTI-FOCAL MASSES, ONE IS 40 X 30 X 20 MM, TAN, -B-FIBROADENOMA,-NEOPLASM PRESENT FIRM AND LOBULATED; SAME ON CUT SURFACE; THE OTHER IS 20 X 18 X 10 MM, RED, DARK RED, TAN AND SOFT; ON SECTION EXUDES A DARK RED CREAMY SEMIFLUID; BOTH COLLECTED INTACT; REGIONAL LN (INGUINAL) NORMAL (CAP 21) CERVIX (CV) : -DILATATION,-MODERATE, FOCAL -B-POLYP, ENDOMETRIAL STROMAL,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, INGUINAL (IN) : >TISSUE MISSING GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 3275 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1668 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91482 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 281.6 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 11:45 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3276 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1669 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91483 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 11:05 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYE-RIGHT; EYE (EY) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC-ACTIVE, CORNEAL,- >APPEARANCE; SWOLLEN; LIMB-HIND-RIGHT; MINIMAL, MULTI-FOCAL VERIFIED AT NECROPSY: NOT OBSERVED -VASCULARIZATION, CORNEAL,-SLIGHT, MULTI-FOCAL BRAIN (BR) : -VACUOLATION, WHITE MATTER,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL SPINAL CORD (SC) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 7 X 5 X 3 MM; APPEARS SWOLLEN -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL 3277 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1670 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91483 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 11:05 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MINERALIZATION,-MINIMAL, MULTI-FOCAL UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, -LARGE; BILATERAL: EACH IS 10 X 6 X 3 DIFFUSE MM; COLLECTED GENERAL COMMENT (GC) : -DISCHARGE-ORAL; DARK RED AND DRY -DISCHARGE-OCULAR; RIGHT: DARK RED AND DRY -------------------------------------------- 3278 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1671 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91483 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 11:05 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3279 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1672 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91484 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/31/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 186.1 GRAMS DATE AND TIME OF NECROPSY: 05/31/99 10:29 PROSECTOR: JOHN S. HALFORD RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: OBSERVED ADRENAL, CORTEX (AC) : >APPEARANCE; HUNCHED; VERIFIED AT -DEGENERATION, CYSTIC, CORTICAL,- NECROPSY: NOT APPLICABLE MODERATE, MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT THYMUS (TH) : NECROPSY: NOT APPLICABLE >SECTION EXAMINED; TISSUE NOT PRESENT >SKIN & PELAGE; PALE; ENTIRE BODY; LUNG (LU) : VERIFIED AT NECROPSY: NOT APPLICABLE -MINERALIZATION, VASCULAR,-MINIMAL, >SKIN & PELAGE; COLD TO TOUCH; ENTIRE FOCAL BODY; VERIFIED AT NECROPSY: NOT PITUITARY (PI) : PITUITARY (PI) : APPLICABLE -MASS(ES); 11 X 9 X 8 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL GRAY, LOBULATED, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE BROWN AREAS, UP TO 2 X 1 MM MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS 3280 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1673 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91484 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/31/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 186.1 GRAMS DATE AND TIME OF NECROPSY: 05/31/99 10:29 PROSECTOR: JOHN S. HALFORD RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3281 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1674 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91485 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 438.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:01 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- MULTIPLE DARK RED AREAS, UP TO 4 X 3 FOCAL MM; COLLECTED ON ROUTINE SECTIONS -TELANGIECTASIS,-MINIMAL, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- MULTIPLE TAN AREAS, UP TO 5 MM IN FOCAL DIAMETER -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); ANTERIOR -HEMORRHAGE,-MINIMAL, MULTI-FOCAL ASPECT: SINGLE BLACK AREA, 5 X 3 MM -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); RIGHT PERINEAL REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL MULTIPLE WHITE, TAN, AND CREAMY, -B-FIBROADENOMA,-NEOPLASM PRESENT SEMISOLID FILLED CYSTS, UP TO 15 MM IN DIAMETER; (CAP 20) 3282 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1675 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91485 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 438.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:01 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: DARK RED, AND TAN -HEMORRHAGE,-SLIGHT, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: BROKEN OFF AT BASE -------------------------------------------- 3283 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1676 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91485 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 438.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:01 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3284 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1677 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91486 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 261.2 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 15:00 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; OPAQUE; EYE-RIGHT; VERIFIED AT EYE (EY) : EYE (EY) : NECROPSY: OBSERVED -OPAQUE CORNEA; RIGHT -SYNECHIA, ANTERIOR,-PRESENT >APPEARANCE; MISSING; TAIL-PROXIMAL; -VASCULARIZATION, CORNEAL,-SLIGHT, VERIFIED AT NECROPSY: OBSERVED FOCAL >SKIN & PELAGE; PALE; ENTIRE BODY; >NOTE:>RIGHT EYE VERIFIED AT NECROPSY: NOT APPLICABLE BRAIN (BR) : BRAIN (BR) : >APPEARANCE; THIN; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : >SKIN & PELAGE; YELLOW HAIR COAT; -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- VERIFIED AT NECROPSY: NOT OBSERVED FOCAL >BEHAVIOR; ATAXIC; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -HYPERPLASIA, TRANSITIONAL CELL,- >APPEARANCE; SWOLLEN; MOUTH; VERIFIED SLIGHT, MULTI-FOCAL AT NECROPSY: NOT OBSERVED -MINERALIZATION, PELVIC,-MODERATE, >EXCRETION; FEW FECES; VERIFIED AT MULTI-FOCAL NECROPSY: NOT APPLICABLE PANCREAS (PA) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MINIMAL, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 3285 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1678 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91486 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 261.2 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 15:00 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 MM IN DIAMETER, DARK -PIGMENT,-MINIMAL, MULTI-FOCAL RED, TAN, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS UP TO 12 X -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, 8 X 3 MM; (CAP 21) MULTI-FOCAL LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: EACH IS UP TO 11 X -HYPERPLASIA, LYMPHOCYTIC,-MINIMAL, 6 X 3 MM (CAP 22) MULTI-FOCAL LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; BILATERAL: EACH IS UP TO 10 X -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, 4 X 3 MM (CAP 23) MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -TAIL - BENT/MISSING; TIP: MISSING, PORTION OF REMAINING TAIL COLLECTED -------------------------------------------- 3286 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1679 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91486 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 261.2 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 15:00 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3287 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1680 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91487 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 682.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:31 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 7 X 5 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN, AND FIRM -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE RED AREAS, UP TO 1 MM IN DIAMETER MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL 3288 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1681 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91487 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 682.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:31 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL UTERUS (UT) : UTERUS (UT) : -CYST(S); WITHIN LUMEN OF LEFT HORN, -DILATATION,-MODERATE, FOCAL 14 MM FROM BIFURCATION: SINGLE DARK -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- RED FLUID FILLED CYST, 4 MM IN MODERATE, MULTI-FOCAL DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -HEMORRHAGE,-MODERATE, MULTI-FOCAL (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 3289 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1682 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91487 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 682.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:31 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 3290 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1683 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91488 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 507.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:09 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: >UNREMARKABLE MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER, COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI- FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID >UNREMARKABLE FILLED CYST, 5 MM IN DIAMETER LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL UTERUS (UT) : UTERUS (UT) : -CYST(S); DISTAL END OF LEFT HORN, -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- WITHIN WALL: SINGLE CLEAR FLUID FILLED MODERATE, MULTI-FOCAL CYST, 8 X 5 X 5 MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3291 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1684 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91488 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 507.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:09 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; -DILATATION, SINUSOIDAL,-SLIGHT, MULTI- COLLECTED FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3292 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1685 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91489 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/09/99 13:36 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK EYE (EY) : EYE (EY) : 79; 20 X 15MM; VERIFIED AT NECROPSY: -WHITE INTRAOCULAR MATERIAL; LEFT >UNREMARKABLE OBSERVED LIVER (LI) : LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- -RED FOCUS(I)/AREA(S); ALL LOBES: FOCAL MULTIPLE DARK RED AREAS, UP TO 4 X 6 MM (COLLECTED WITH ROUTINE SECTIONS) KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI- FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SINGLE DARK RED AREA, 2 X 4 MM -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT CERVICAL REGION: -GALACTOCELE,-MINIMAL, MULTI-FOCAL SINGLE MASS, 11 X 20 X 25 MM, FIRM, -B-FIBROADENOMA,-NEOPLASM PRESENT RED, TAN AND LOBULATED; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (MANDIBULAR) NORMAL (CAP 22) 3293 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1686 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91489 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/09/99 13:36 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : >TISSUE MISSING LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -DIFFUSELY RED; DARK RED (CAP 23) -HEMORRHAGE,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -FLUID-THORACIC CAVITY; FILLED WITH A DARK RED FLUID AND A DARK RED CLOTTED MATERIAL -------------------------------------------- 3294 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1687 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91489 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/09/99 13:36 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3295 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1688 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91490 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 417.4 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 12:11 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-THORACIC-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 75; 20 X 30MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; SORE/SCAB; VENTRAL- -DIFFUSELY LIGHT; ALL LOBES: TAN >UNREMARKABLE ABDOMINAL; VERIFIED AT NECROPSY: KIDNEY (KD) : OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >APPEARANCE; MALOCCLUSION; VERIFIED AT FOCAL NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, FOCAL >SKIN & PELAGE; SORE/SCAB; VENTRAL- SPLEEN (SP) : THORACIC-RIGHT; VERIFIED AT NECROPSY: -HEMATOPOIESIS, EXTRAMEDULLARY, OBSERVED INCREASED,-MODERATE, DIFFUSE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, DARK RED, TAN -HEMORRHAGE,-MODERATE, MULTI-FOCAL AND SOFT -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -ULCERATION,-SEVERE, FOCAL 3296 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1689 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91490 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 417.4 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 12:11 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : -MASS(ES); VENTRAL ABDOMINAL REGION: SINGLE MASS, 20 X 17 X 8 MM, TAN AND FIRM; ON SECTION, EXUDES A TAN CASEOUS SEMISOLID MATERIAL; OVERLYING SKIN HAS A DRY AND DARK RED CRUSTED AREA, 15 MM IN DIAMETER; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT VENTRAL THORACIC -GALACTOCELE,-MINIMAL, MULTI-FOCAL REGION: SINGLE MASS, 25 X 25 X 15 MM, -M-CARCINOMA,- 2 NEOPLASMS PRESENT TAN AND SOFT; SAME ON CUT SURFACE; OVERLYING SKIN HAS A MOIST, DARK RED AND CRUSTED ULCERATED AREA; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 20) CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -ANIMAL OBESE -------------------------------------------- 3297 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 PAGE: 1690 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C91490 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 417.4 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 12:11 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3298 APPENDIX 7 Cell Proliferation Report Covance 6329-212 3M T-6316.1 Note: This appendix contains information supplied by the Sponsor and has been reviewed by the Quality Assurance Unit of Pathology Associates International. 3299 Covance 6329-212 3M T-6316.1 3300 Covance 6329-212 3M T-6316.1 3301 Covance 6329-212 3M T-6316.1 3302 Covance 6329-212 3M T-6316.1 3303 Covance 6329-212 3M T-6316.1 3304 Covance 6329-212 3M T-6316.1 3305 Covance 6329-212 3M T-6316.1 3306 Covance 6329-212 3M T-6316.1 3307 Covance 6329-212 3M T-6316.1 3308 Covance 6329-212 3M T-6316.1 3309 Covance 6329-212 3M T-6316.1 3310 Covance 6329-212 3M T-6316.1 3311 Covance 6329-212 3M T-6316.1 3312 Covance 6329-212 3M T-6316.1 3313 Covance 6329-212 3M T-6316.1 3314 Covance 6329-212 3M T-6316.1 3315 Covance 6329-212 3M T-6316.1 3316 Covance 6329-212 3M T-6316.1 3317 Covance 6329-212 3M T-6316.1 3318 Covance 6329-212 3M T-6316.1 3319 Covance 6329-212 3M T-6316.1 3320 Covance 6329-212 3M T-6316.1 3321 Covance 6329-212 3M T-6316.1 3322 Covance 6329-212 3M T-6316.1 3323 Covance 6329-212 3M T-6316.1 3324 Covance 6329-212 3M T-6316.1 3325 Covance 6329-212 3M T-6316.1 3326 Covance 6329-212 3M T-6316.1 3327 Covance 6329-212 3M T-6316.1 3328 Covance 6329-212 3M T-6316.1 3329 Covance 6329-212 3M T-6316.1 3330 Covance 6329-212 3M T-6316.1 3331 Covance 6329-212 3M T-6316.1 3332 Covance 6329-212 3M T-6316.1 3333 Covance 6329-212 3M T-6316.1 3334 Covance 6329-212 3M T-6316.1 3335 Covance 6329-212 3M T-6316.1 3336 Covance 6329-212 3M T-6316.1 3337 APPENDIX 8 Electron Microscopy Report Covance 6329-212 3M T-6316.1 Note: This appendix contains information supplied by the Pathology Associates International and has been reviewed by the Quality Assurance Unit of Pathology Associates International. 3338 Covance 6329-212 3M T-6316.1 3339 Covance 6329-212 3M T-6316.1 3340 Covance 6329-212 3M T-6316.1 3341 Covance 6329-212 3M T-6316.1 3342 Covance 6329-212 3M T-6316.1 3343 Covance 6329-212 3M T-6316.1 3344 Covance 6329-212 3M T-6316.1 3345 Covance 6329-212 3M T-6316.1 3346 Covance 6329-212 3M T-6316.1 3347 Covance 6329-212 3M T-6316.1 3348 Covance 6329-212 3M T-6316.1 3349 Covance 6329-212 3M T-6316.1 3350 Covance 6329-212 3M T-6316.1 3351 Covance 6329-212 3M T-6316.1 3352 Covance 6329-212 3M T-6316.1 3353 Covance 6329-212 3M T-6316.1 3354 Covance 6329-212 3M T-6316.1 3355 Covance 6329-212 3M T-6316.1 3356 Covance 6329-212 3M T-6316.1 3357 Covance 6329-212 3M T-6316.1 3358 Covance 6329-212 3M T-6316.1 3359 Covance 6329-212 3M T-6316.1 3360 Covance 6329-212 3M T-6316.1 3361 Covance 6329-212 3M T-6316.1 3362 Covance 6329-212 3M T-6316.1 3363 Covance 6329-212 3M T-6316.1 3364 Covance 6329-212 3M T-6316.1 3365 Covance 6329-212 3M T-6316.1 3366 Covance 6329-212 3M T-6316.1 3367 Covance 6329-212 3M T-6316.1 3368 Covance 6329-212 3M T-6316.1 3369 Covance 6329-212 3M T-6316.1 3370 Covance 6329-212 3M T-6316.1 3371 Covance 6329-212 3M T-6316.1 3372 Covance 6329-212 3M T-6316.1 3373 Covance 6329-212 3M T-6316.1 3374 Covance 6329-212 3M T-6316.1 3375 Covance 6329-212 3M T-6316.1 3376 Covance 6329-212 3M T-6316.1 3377 Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Author: Peter J. Thomford, PhD Study Completion Date: December 13, 2001 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-212 Sponsor Study Identification: 3M T-6316.1 Volume IX of XII Page 3378 of 4873 Covance 6329-212 3M T-6316.1 VOLUME IX OF XII CONTENTS Page APPENDIX 9............................................................................................................ 3380 Covance 6329-228................................................................................................. 3381 3379 APPENDIX 9 Covance 6329-228 Covance 6329-212 3M T-6316.1 3380 Covance 6329-212 Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Author: Peter J. Thomford, PhD Study Completion Date: December 13, 2001 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-228 Sponsor Study Identification: 3M T-6316.1 Page 3381 of 4873 Covance 6329-212 Covance 6329-228 3M T-6316.1 COMPLIANCE STATEMENT 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats All aspects of this study were in accordance with the Food and Drug Administration Good Laboratory Practice Regulations as set forth in Title 21 of the Unites States Code of Federal Regulations, Part 58, issued December 22, 1978 (effective June 20, 1979), and with any applicable amendments. 3382 Covance 6329-212 Covance 6329-228 3M T-6316.1 QUALITY ASSURANCE STATEMENT This report, with the exception of Appendix 6, has been reviewed by the Quality Assurance Unit of Covance Laboratories Inc., in accordance with the Food and Drug Administration (FDA) Good Laboratory Practice Regulations, 21 CFR 58. The following inspections were conducted and findings reported to the study director and study director management. Inspection Dates From To 01 Apr 98 01 May 98 28 Apr 98 28 Apr 98 11 May 98 11 May 98 20 Jul 98 20 Jul 98 12 Oct98 12 Oct 98 04 Jan 99 04 Jan 99 03 May 99 03 May 99 02 Jun 99 02 Jun 99 26 Jul 99 26 Jul 99 09 Nov 99 08 Nov 99 26 Apr 00 26 Apr 00 11 May 00 16 May 00 25 Apr 00 24 May 00 25 Apr 00 01 Jun 00 25 Jul 00 11 Aug 00 11 Aug 00 11 Aug 00 11 Aug 00 11 Aug 00 11 Aug 00 11 Aug 00 30 Apr 01 01 May 01 13 Nov 01 13 Nov 01 13 Nov 01 13 Nov 01 Phase Protocol Review Sample Collection Body Weights Animal Observations Test Article Administration Food Consumption Body Weights Protocol Amendment Review Body Weights Food Consumption Postlife Data Review Data Review Data Review Report Review Protocol Amendment Review Protocol Amendment Review Protocol Amendment Review Report Review Protocol Amendment Review Protocol Amendment Review Date Reported to Study Director and Study Director Management 01 Apr 98 28 Apr 98 11 May 98 20 Jul 98 12 Oct 98 04 Jan 99 03 May 99 04 Jun 99 26 Jul 99 08 Nov 99 26 Apr 00 19 May 00 24 May 00 01 Jun 00 11 Aug 00 11 Aug 00 11 Aug 00 11 Aug 00 03 May 01 13 Nov 01 13 Nov 01 3383 Covance 6329-212 Covance 6329-228 3M T-6316.1 STUDY IDENTIFICATION 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Test Material: Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol Sponsor: 3M Toxicology Services Building 220-2E-02, 3M Center St. Paul, Minnesota 55144-1000 Study Monitor: Andrew M. Seacat, PhD 3M 651.575.3161 Alternate Study Monitor: John Butenhoff, PhD, DABT 3M 651.733.1962 Study Location: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Study Director: Peter J. Thomford, PhD Covance Laboratories Inc. PO Box 7545 Madison, Wisconsin 53707-7545 608.241.7207 Study Timetable: Study Initiation Date: Inlife Start Date: Inlife End Date: Study Completion Date: April 1, 1998 April 6, 1998 April 4, 2000 December 13, 2001 3384 Covance 6329-212 Covance 6329-228 3M T-6316.1 KEY PERSONNEL 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Study Director: Study Toxicologist: Study Coordinator: Supervisor, Small-Animal Toxicology: Supervisor, Dose Formulation: Supervisor, Chemistry/Tox Support: Associate Director, Laboratory Animal Medicine: Clinical Pathologist: Supervisor, Clinical Pathology: Anatomic Pathologist: Supervisor, Anatomic Pathology: Supervisor, Anatomic Pathology: Senior Staff Scientist: Biostatistician: Principal Investigator, Cell Proliferation Peter J. Thomford, PhD Thomas E. Ryan, BS Cindy L. Powers, BS Jeni Barman, AAS, ALAT, LAT Dixie Bushee, BS, LATG Mark Hoffmann, BS, MBA Donna J. Clemons, DVM Diplomate, ACLAM Robert L. Hall, DVM, PhD Diplomate, ACVP (Clinical Pathology) Ronald Markevitch, BS, MT (ASCP) James L. Carter, DVM, PhD Diplomate, ACVP Kimberly W. Durland, BS, HT Laurie J. Schuller, BA, LAT Cynthia Y.C. Liu, MA Ajit K. Thakur, PhD Carolyn Moyer, DVM Diplomate, ACVP Pathology Associates International 3385 Covance 6329-212 Covance 6329-228 3M T-6316.1 VOLUME IX OF XII CONTENTS Page ABSTRACT.............................................................................................................. 3390 PURPOSE................................................................................................................. 3392 REGULATORY COMPLIANCE.............................................................................. 3392 TEST MATERIAL, VEHICLE, and CARRIER ........................................................ 3392 Test Material ......................................................................................................... 3392 Vehicle.................................................................................................................. 3393 Carrier ................................................................................................................... 3393 Reserve (Archive) Samples.................................................................................... 3393 Disposition ............................................................................................................ 3393 TEST ANIMALS AND HUSBANDRY.................................................................... 3393 Animals................................................................................................................. 3393 Identification ......................................................................................................... 3394 Justification ........................................................................................................... 3394 Husbandry............................................................................................................. 3394 Acclimation........................................................................................................... 3395 PROCEDURES......................................................................................................... 3395 Group Designations and Dose Levels .................................................................... 3395 Dosing Procedures................................................................................................. 3396 Dose Analysis........................................................................................................ 3397 Observation of Animals......................................................................................... 3398 Clinical Pathology ................................................................................................. 3398 Serum Analyses..................................................................................................... 3399 Anatomic Pathology .............................................................................................. 3400 Proliferation Cell Nuclear Antigen Evaluation....................................................... 3401 Palmitoyl-CoA Oxidase Tissue Collection and Analyses ....................................... 3401 Statistical Evaluation ............................................................................................. 3403 Survival and Tumor Analysis ................................................................................ 3403 Record Retention................................................................................................... 3405 RESULTS AND DISCUSSION ................................................................................ 3405 Dose Analysis........................................................................................................ 3405 Observation of Animals......................................................................................... 3406 Clinical Pathology ................................................................................................. 3407 Anatomic Pathology .............................................................................................. 3407 Proliferation Cell Nuclear Antigen Results ............................................................ 3407 CONCLUSION......................................................................................................... 3408 3386 Covance 6329-212 Covance 6329-228 3M T-6316.1 VOLUME IX OF XII CONTENTS (Continued) Page SIGNATURES.......................................................................................................... 3409 REFERENCES.......................................................................................................... 3410 PATHOLOGY REPORT........................................................................................... 3411 STATISTICAL REPORT OF SURVIVAL, NONNEOPLASTIC, AND NEOPLASTIC LESIONS ...................................................................................... 3415 COMMENTS ON THE DATA ................................................................................. 3425 CODES, ABBREVIATIONS, AND UNITS.............................................................. 3427 General Codes and Abbreviations.......................................................................... 3428 Codes for Clinical Pathology................................................................................. 3429 Abbreviations and Units for Clinical Hematology.................................................. 3432 Abbreviations and Units for Clinical Chemistry..................................................... 3433 Abbreviations and Units for Clinical Urinalysis..................................................... 3434 Abbreviations and Units for Urine Chemistry ........................................................ 3434 Codes for Anatomic Pathology .............................................................................. 3435 FIGURES 1 Adjusted Survival Data (%) - Males................................................................. 3437 2 Adjusted Survival Data (%) - Females ............................................................. 3438 3 Mean Body Weight Data (g) - Males ............................................................... 3439 4 Mean Body Weight Data (g) - Females ............................................................ 3440 5 Mean Food Consumption Data (g) - Males ...................................................... 3441 6 Mean Food Consumption Data (g) - Females ................................................... 3442 TABLES 1 Results of Homogeneity Analyses (ppm) ......................................................... 3443 2 Results of Stability Analyses (ppm) ................................................................. 3444 3 Results of Dose Preparation Analyses (ppm).................................................... 3446 4 Survival Data................................................................................................... 3455 5 Summary of Clinical Observations................................................................... 3464 6 Summary of Mass Observations....................................................................... 3471 7 Summary of Body Weight Data (g).................................................................. 3480 8 Summary of Body Weight Change Data (g)..................................................... 3486 9 Summary of Food Consumption Data (g)......................................................... 3487 10 Summary of Test Material Consumption Data (mg/kg/day).............................. 3493 11 Summary of Clinical Hematology Data - Week 14........................................... 3499 12 Summary of Clinical Hematology Data - Week 27........................................... 3503 13 Summary of Clinical Hematology Data - Week 53........................................... 3507 3387 Covance 6329-212 Covance 6329-228 3M T-6316.1 VOLUME IX OF XII CONTENTS (Continued) Page TABLES 14 Summary of Clinical Chemistry Data - Week 4 ............................................... 3511 15 Summary of Clinical Chemistry Data - Week 14.............................................. 3513 16 Summary of Clinical Chemistry Data - Week 27.............................................. 3519 17 Summary of Clinical Chemistry Data - Week 53.............................................. 3523 18 Summary of Clinical Urinalysis Data - Week 14.............................................. 3527 19 Summary of Clinical Urinalysis Data - Week 27.............................................. 3529 20 Summary of Clinical Urinalysis Data - Week 53.............................................. 3531 21 Summary of Urine Chemistry Data - Week 14 ................................................. 3533 22 Summary of Urine Chemistry Data - Week 27 ................................................. 3535 23 Summary of Urine Chemistry Data - Week 53 ................................................. 3537 24 Summary of Organ Weight Data - Week 4 Sacrifice ........................................ 3539 25 Summary of Organ Weight Data - Week 14 Sacrifice ...................................... 3540 26 Summary of Organ Weight Data - Week 53 Sacrifice ...................................... 3541 27 Incidence of Macroscopic Observations - Week 4 Sacrifice ............................. 3556 28 Incidence of Macroscopic Observations - Week 14 Sacrifice ........................... 3557 29 Incidence of Macroscopic Observations - Week 53 Sacrifice ........................... 3558 30 Incidence of Macroscopic Observations - Terminal Sacrifice ........................... 3569 31 Incidence of Macroscopic Observations - Unscheduled Deaths ........................ 3583 32 Incidence of Macroscopic Observations - All Animals..................................... 3601 33 Incidence of Microscopic Observations - Week 53 Sacrifice............................ 3619 34 Incidence of Microscopic Observations - Terminal Sacrifice............................ 3642 35 Incidence of Microscopic Observations - Unscheduled Deaths......................... 3673 36 Incidence of Microscopic Observations - All Animals ..................................... 3709 37 Incidence of Severity of Selected Microscopic Observations - Week 53 Sacrifice........................................................................................................ 3749 38 Incidence of Severity of Selected Microscopic Observations - Terminal Sacrifice........................................................................................................ 3753 39 Incidence of Severity of Selected Microscopic Observations - Unscheduled Deaths .......................................................................................................... 3758 40 Incidence of Severity of Selected Microscopic Observations - All Animals...... 3763 APPENDIX 1............................................................................................................ 3768 Protocol Deviations ............................................................................................... 3769 3388 Covance 6329-212 Covance 6329-228 3M T-6316.1 VOLUME X OF XII CONTENTS (Continued) Page APPENDIX 2............................................................................................................ 3774 Individual Animal Fate Data.................................................................................. 3775 Individual Clinical Observations............................................................................ 3784 APPENDIX 3............................................................................................................ 4017 Individual Body Weight Data (g)........................................................................... 4018 APPENDIX 4............................................................................................................ 4051 Individual Food Consumption Data (g).................................................................. 4052 Individual Test Material Consumption Data (mg/kg/day)....................................... 4085 APPENDIX 5............................................................................................................ 4105 Individual Clinical Hematology Data..................................................................... 4106 Individual Clinical Chemistry Data........................................................................ 4124 Individual Clinical Urinalysis Data........................................................................ 4140 Individual Urine Chemistry Data ........................................................................... 4158 VOLUME XI OF XII APPENDIX 6............................................................................................................ 4166 Individual Anatomic Pathology Data ..................................................................... 4167 VOLUME XII OF XII APPENDIX 6 (Continued) ........................................................................................ 4508 Individual Anatomic Pathology Data ..................................................................... 4509 APPENDIX 7............................................................................................................ 4857 Cell Proliferation Report ....................................................................................... 4858 3389 ABSTRACT Covance 6329-212 Covance 6329-228 3M T-6316.1 The purpose of this study was to assess the carcinogenicity of the test material, N-Ethyl Perfluorooctanesulfonamido Ethanol (N-EtFOSE), when administered in the diet to rats for at least 104 weeks. This study was a supplementary study for Covance 6329-212. Male and female Crl:CD(SD)IGS BR rats were assigned to two groups (70 animals/sex/group). Group 8 received the control diet and Group 9 received diet containing 1 ppm N-EtFOSE.1. Food was provided ad libitum, except when animals were fasted. Water was provided ad libitum. The animals were observed twice daily (a.m. and p.m.) for mortality and moribundity. At least once each week, each animal was removed from its cage and examined for abnormalities and signs of toxicity. Body weight data were collected weekly through Week 17, once every 4 weeks thereafter, and at Week 105. Food consumption data were collected weekly for the first 16 weeks and once every 4 weeks thereafter. Blood and urine samples were collected for hematology, coagulation, clinical chemistry, urinalysis, and urine chemistry tests at specified time points. Blood was also collected to provide serum for possible future analysis. During Weeks 4, 14, 53, and 105, selected animals were anesthetized, weighed, exsanguinated, and necropsied (Weeks 53 and 105). Additional liver samples were also collected for hepatocellular proliferation (Week 14) and biochemical analyses from five animals/sex/group (Weeks 4 and 14). Liver sections from five animals/sex/group from Group 9 were stained with Oil Red "O" and examined microscopically. At necropsy, macroscopic observations were recorded, selected organs were weighed (Week 53), and selected tissues were collected and preserved. Animals that died on test or were sacrificed at unscheduled intervals were also necropsied, but organ weights were not recorded. Microscopic examinations were done on tissues from each animal in Groups 8, and any animals that died on test or were sacrificed at unscheduled intervals. Lesions, liver, lungs, kidneys, pancreas, thyroid, testes, and mammary glands (females) from each animal in Group 9 were examined microscopically. Test material consumption ranged from 0.029 to 0.108 mg/kg/day for Group 9. Thirty-nine control males and 37 control females and 30 males and 35 females given 1 ppm died on test or were sacrificed in a moribund condition. There were no significant 3390 Covance 6329-212 Covance 6329-228 3M T-6316.1 differences in the mortality in both males and females. Kaplan-Meier estimates indicate there was no treatment-related detrimental effect on the survival of either sex. Male and female Crl:CD(SD)IGS BR rats were administered 0 or 1 ppm of (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol for at least 104 weeks. There were no test material-related effects on clinical observations, body weights, food consumption, clinical pathology results, organ weights, macroscopic or microscopic findings. 3391 PURPOSE Covance 6329-212 Covance 6329-228 3M T-6316.1 The purpose of this study was to assess the carcinogenicity of the test material, N-Ethyl Perfluorooctanesulfonamido Ethanol (N-EtFOSE), when administered in the diet to rats for at least 104 weeks. This study was a supplementary study for Covance 6329-212. REGULATORY COMPLIANCE All aspects of this study were conducted in compliance with the Food and Drug Administration Good Laboratory Practice Regulations as set forth in Title 21 of the United States Code of Federal Regulations, Part 58, issued December 22, 1978 (effective June 20, 1979), and with any applicable amendments. The protocol was reviewed and approved by the Institutional Animal Care and Use Committee at Covance. TEST MATERIAL, VEHICLE, AND CARRIER Test Material The test material, T-6316 (narrow range N-ethyl perfluorooctanesulfonamido ethanol, N-EtFOSE), a blend of Lot Nos. 30035, 30037, and 30039, is a waxy solid and is 98.1% N-EtFOSE (w/w) (see Protocol Deviations for exceptions). It was received at Covance on June 20 and July 21, 1997. To avoid repeated heating and sublimation, the two shipments of test material were heated, combined, and predetermined quantities of test material were aliquoted into polyethylene (LDPE) containers. The test material was stored at room temperature. Information on synthesis methods, stability, composition, or other characteristics that define the test material was provided by the Sponsor and is on file with Covance. 3392 Covance 6329-212 Covance 6329-228 3M T-6316.1 Vehicle The vehicle was acetone (Spectrum Chemical Mfg. Corp., New Brunswick, New Jersey and Gardena, California), Lot Nos. LW0044 (99.96% pure), LH0253 (99.8% pure), and NS0231 (99.8% pure). The shipments were received at Covance on April 9 and June 23, 1997, and September 8, 1998, respectively. The vehicle was stored at room temperature. Information on synthesis methods, purity, composition, or other characteristics that define the acetone is on file with the manufacturer. Carrier Certified rodent diet (#5002 meal, LabDiet by Purina Mills, Inc.) was used as supplied. The lot numbers are recorded in the data. The diet is routinely analyzed by the manufacturer for nutritional components and environmental contaminants. Reserve (Archive) Samples A reserve sample (5 g) of the test material was taken and stored at room temperature. This sample will be transferred to the Sponsor after completion of the inlife phase. Disposition Remaining test material was returned to the Sponsor on August 8, 2000. TEST ANIMALS AND HUSBANDRY Animals Male and female Crl:CD(SD)IGS BR rats were obtained from the Raleigh, North Carolina, facility of Charles River Laboratories on March 24, 1998. At initiation of treatment, the animals were 38 to 44 days old; the males weighed from 163 to 240 g, and the females weighed from 126 to 179 g. 3393 Covance 6329-212 Covance 6329-228 3M T-6316.1 Identification Each animal was assigned a temporary number upon arrival. Before initiation of treatment, a microchip identification device was implanted into each animal. After randomization for placement on test, each animal was assigned a permanent number, and the microchip was coded with the permanent number. All data for an animal are recorded under these numbers. Justification Historically, rats have been used in safety evaluation studies and are recommended by appropriate regulatory agencies. Husbandry Animal Rooms 3045 and 335 were used for this study. Environmental controls for the animal room were set to maintain 18 to 26oC, a relative humidity of 30 to 70%, a 12-hour light/12-hour dark cycle, and a minimum of 10 room air changes/hour. Variations from these conditions are documented in the data and are considered to have had no effect on the outcome of the study. The animals were housed individually (except for the first 6 days of acclimation when animals were group-housed) in stainless-steel cages. Some animals were placed in polycarbonate cages during the study when health problems dictated. Certified rodent diet (#5002 meal, LabDiet by Purina Mills, Inc.) was provided ad libitum during acclimation. Water was provided ad libitum. Samples of the water are analyzed for specified microorganisms and environmental contaminants. The results are on file with Covance-Madison. There were no known contaminants in the diet or water at levels that would have interfered with this study. 3394 Covance 6329-212 Covance 6329-228 3M T-6316.1 Animals not selected for the study (15 males and 15 females) were used for training procedures. Two extra males and one extra female were received and also used for training procedures. Acclimation One hundred fifty-seven males and 156 females were received on March 24, 1998, and acclimated in Animal Rooms 320 and 3045 for 13 days before initiation of treatment (animals were transferred to Animal Room 3045 on April 3, 1998). In general, animals in this shipment appeared healthy. During acclimation, the animals were examined for abnormalities indicative of health problems and body weights were recorded for all animals at randomization. PROCEDURES This study was conducted in accordance with the Protocol dated April 1, 1998, and Protocol Amendment Nos. 1 through 6. Protocol deviations are in Appendix 1. Group Designations and Dose Levels The animals used for this study were examined by a laboratory animal veterinarian on April 3, 1998, and found to be suitable for study consideration. Selection of animals for the study was based on acclimation data. Animals were assigned to treatment groups using a computerized blocking procedure designed to achieve body weight balance with respect to treatment group. At the time of randomization, the weight variation of the animals did not exceed +2 standard deviations of the mean body weight for each sex. Group mean body weights were analyzed using Levene's test for homogeneity of variance at the 5.0% probability level and found to be homogeneous. Animals were assigned to the study according to the following design. 3395 Covance 6329-212 Covance 6329-228 3M T-6316.1 Groupa 8 (Control)c,d,e 9 (Treated)d,e No. of Animals Male Female 70 70 70 70 Dietary Levels (ppm N-EtFOSE)b 0 1 a Groups were designated as Groups 8 and 9. This study was placed in the same animal room as Covance 6329-212. b N-EtFOSE is 98.1% n-ethyl perfluorooctanesulfonamido ethanol (N-EtFOSE); dose levels are expressed as ppm of N-EtFOSE. c The control animals received the control diet (basal diet with acetone). d Five animals/sex/group were sacrificed during Weeks 4 and 14 for hepatocellular proliferation rate measurements and biochemical analyses (palmitoyl-CoA oxidation). e Ten animals/sex/group were designated as interim sacrifice animals and were sacrificed after at least 52 weeks of treatment. Dosing Procedures Dose Preparation. Dietary concentrations were based on the test material as supplied. Diets were prepared at least once every 4 weeks. Preparation of Diet for Group 8. The appropriate amount of certified diet was weighed and mixed with an appropriate volume of acetone. Preparation of Stock and Base Diet. Diets were prepared in conjunction with Covance 6329-212. A stock preparation was made using the required amount of test material dissolved in the required volume of acetone. For the 300-ppm base diet, a premix consisting of a portion of the certified diet with the required amount of the stock preparation was mixed using a Hobart mixer. The premix was transferred to a mixer with additional certified diet and mixed thoroughly. Diet for Group 9. For the 1-ppm diet, a premix consisting of a portion of the certified diet, the required amount of the prepared base diet, and the required volume of acetone was mixed and then transferred to a mixer with additional certified diet and mixed thoroughly. Samples for dose analyses were taken directly from the mixing bowl. 3396 Covance 6329-212 Covance 6329-228 3M T-6316.1 The diets were stored at room temperature in covered containers until dispensed into feeding jars. Method of Administration. Dietary admixture was used because the potential route of exposure in humans is oral. The dose preparations were administered ad libitum. Animals received the test diet for at least 104 weeks, as appropriate(see Protocol Deviations for exceptions). Retention Samples. Samples (approximately 100 g) were taken from each dose preparation, during the inlife phase, and stored at room temperature. Unless used for analyses, retention samples from the treated groups were discarded at least 1 month after completion of the inlife phase. Samples from the control diets prepared for Weeks 1 through 14 were shipped to the Sponsor. Dose Analysis Analyses for the concentration of test material in the dose preparations were done by Covance using an analytical method, MP-M312-MA, validated by Covance. Homogeneity was determined for the 1-ppm diet mixed for Week 1. One sample (approximately 100 g each ) from the top, middle, and bottom of the diet was collected, divided into three subsamples for extraction and analysis, and analyzed for test material content. All samples were stored at room temperature until analyzed. To establish stability, two additional samples (approximately 100 g each) were taken from the 1-ppm diet mixed for Week 1. The middle homogeneity sample was analyzed on the day of mixing and served as the stability time zero sample. One sample was stored at room temperature for 21 days, then analyzed. The other sample was stored at room temperature for 32 days, then analyzed. Stability analyses were also conducted on another mix (June 5, 1998). The routine sample from that mix served as the time zero sample. Samples were stored at room temperature for 32 days or 46 days at room temperature, then analyzed. Samples (approximately 100 g each) were collected from all dose preparations and analyzed in duplicate. The homogeneity sample collected from the middle of the dose 3397 Covance 6329-212 Covance 6329-228 3M T-6316.1 preparation for Week 1 was used for dose confirmation results. All samples were stored at room temperature until analyzed. Observation of Animals Clinical Observations. The animals were observed twice daily (a.m. and p.m.) for mortality and moribundity (see Protocol Deviations for exceptions). Once prior to treatment and weekly thereafter, each animal was removed from its cage and examined; abnormal findings or an indication of normal were recorded. In addition, the time of onset, location, size, appearance, and progression of each grossly visible or palpable mass were recorded. Body Weights. Individual body weight data were recorded on Day -3 (randomization), weekly for Weeks 1 through 17 (see Protocol Deviations for exceptions), once every 4 weeks thereafter, and at Week 105. Food Consumption. Individual food consumption data were recorded weekly for Weeks 1 through 16 (see Protocol Deviations for exceptions) and once every 4 weeks thereafter (see Protocol Deviations for exceptions). Clinical Pathology Blood and urine samples were collected from 10 animals/sex/group during Weeks 14, 27, and 53. Blood films were made and held for possible future examination from animals sacrificed at unscheduled intervals and from animals at scheduled sacrifice after at least 104 weeks of treatment. Animals were fasted overnight, and urine was collected chilled for approximately 16 hours before blood sampling; water was provided ad libitum. Blood was collected from a jugular vein. The anticoagulant was potassium EDTA for the hematology tests. Samples for clinical chemistry and serum samples were collected without an anticoagulant. Animals were bled in random order. The following were evaluated. 3398 Covance 6329-212 Covance 6329-228 3M T-6316.1 Hematology red blood cell (erythrocyte) count white blood cell (leukocyte) count hemoglobin hematocrit differential blood cell count segmented neutrophil count mean corpuscular volume mean corpuscular hemoglobin lymphocyte count monocyte count mean corpuscular hemoglobin concentration eosinophil count basophil count platelet count blood cell morphology Reticulocyte smears were made and held for possible future examination. glucose urea nitrogen creatinine total protein albumin globulin cholesterol total bilirubin appearance volume specific gravity pH protein urobilogen sodium potassium Clinical Chemistry alanine aminotransferase gamma glutamyltransferase aspartate aminotransferase calcium inorganic phosphorus sodium potassium chloride Urinalysis glucose ketones bilirubin blood microscopic examination of sediment Urine Chemistry 16-hour excretion of: sodium potassium Serum Analyses Blood (approximately 2 mL at the Weeks 4 and 14 sacrifices and approximately 3 mL at the Week 53 and 105 sacrifices) was collected. Samples were collected from five animals/sex/group during Weeks 4 and 14 (animals selected for hepatocellular 3399 Covance 6329-212 Covance 6329-228 3M T-6316.1 proliferation and biochemical analyses); five animals/sex/group (from animals selected for interim sacrifice) after at least 52 weeks of treatment; and 10 animals/sex/group at terminal sacrifice. Animals were fasted overnight and blood was collected without an anticoagulant from a jugular vein. Blood samples were allowed to clot at room temperature, then centrifuged. Serum samples were harvested and stored in a freezer set to maintain -60 to -80oC. Samples were shipped on dry ice to the Sponsor for possible future analyses. Anatomic Pathology Necropsy. A necropsy was done on each animal that died or was sacrificed at an unscheduled interval. Animals were sacrificed with carbon dioxide, weighed, and exsanguinated. During Week 4, five animals/sex/group were fasted overnight, bled for serum samples, anesthetized with carbon dioxide, weighed, and exsanguinated. The liver was removed and weighed. Additional liver samples were stored in a freezer, set to maintain -60 to -80oC, and sent to the Sponsor for possible future analysis. During Week 14, five animals/sex/group were fasted overnight, bled for serum samples, anesthetized with carbon dioxide, weighed, and exsanguinated. The liver was removed and weighed. Additional liver samples were stored in a freezer, set to maintain -60 to -80oC, and shipped to the Sponsor for possible future analysis. After at least 52 weeks of treatment, 10 animals/sex/group were fasted overnight, bled for clinical pathology (all animals) and serum samples (five animals/sex/group), anesthetized with carbon dioxide, weighed, exsanguinated, and necropsied. Additional liver samples (five animals/sex/group) were stored in a freezer, set to maintain -60 to -80oC, and shipped to the Sponsor for possible future analysis. After 104 weeks of treatment, the remaining animals were fasted overnight, bled for serum samples (10 animals/sex/group), anesthetized with carbon dioxide, weighed, exsanguinated, and necropsied. Liver samples from 10 animals/sex/group were stored in a freezer, set to maintain -60 to -80oC, and shipped to the Sponsor for possible future analysis. 3400 Covance 6329-212 Covance 6329-228 3M T-6316.1 The necropsy included a macroscopic examination of the external features of the carcass; external body orifices; the abdominal, thoracic, and cranial cavities; organs; and tissues. Proliferation Cell Nuclear Antigen Evaluation At the Week 14 collection, representative samples of left lateral lobe of the liver and macroscopic lesions of the liver were collected and preserved in zinc formalin. After fixation, each sample of the liver was embedded in paraffin, and the paraffin blocks were shipped to Pathology Associates International. Proliferation cell nuclear antigen (PCNA) evaluation were done on the samples. In addition, sections of the left lateral lobe of the liver were stained with hematoxylin and eosin and examined microscopically. Palmitoyl-CoA Oxidase Tissue Collection and Analyses At the Week 4 and 14 sacrifices, sample (approximately 500 mg) of the right lateral lobe of the liver was also collected from each animal and flash-frozen in liquid nitrogen (see Protocol Deviations for exceptions). The liver tissue was stored in a freezer, set to maintain -60 to -80oC, until analyzed for palmitoyl-CoA oxidase activity. Organ Weights. At the interim sacrifice during Week 53, the following organs were weighed (see Protocol Deviations for exceptions); paired organs were weighed separately. adrenal (2) brain kidney (2) liver lung ovary (2) spleen testis (2) thyroid (2) with parathyroid uterus with cervix Organ-to-body weight percentages and organ-to-brain weight ratios were calculated. Tissue Preservation. The following tissues were collected and preserved in 10% neutral-buffered formalin (see Protocol Deviations for exceptions). 3401 adrenal (2) brain cecum cervix colon duodenum epididymis (2) esophagus eye (2) femur with bone marrow (articular surface of the distal end) Harderian gland heart ileum jejunum kidney (2) lesions liver lung with mainstem bronchi lymph node (mesenteric) mammary gland (females only) ovary (2) Covance 6329-212 Covance 6329-228 3M T-6316.1 pancreas pituitary prostate rectum salivary gland [mandibular (2)] sciatic nerve seminal vesicle (2) skeletal muscle (thigh) skin spinal cord (cervical, thoracic, and lumbar) spleen sternum with bone marrow stomach testis (2) thymus thyroid (2) with parathyroid trachea urinary bladder uterus vagina Bone marrow smears from the femur of each animal at the Week 53 and terminal sacrifices were prepared, stained with Wright's stain, and retained for possible examination. Histopathology. Tissues (as appropriate) were embedded in paraffin, sectioned, stained with hematoxylin and eosin, and examined microscopically (see Protocol Deviations for exceptions) from each animal in Group 8 sacrificed at the terminal sacrifice and any animals that died on test or were sacrificed at unscheduled intervals. Lesions, liver, lungs, kidneys, pancreas, thyroid, testes, and mammary glands (females) from each animal in Group 9 were embedded in parafilm, sectioned, stained with hematoxylin and eosin, and examined microscopically. Parathyroids were processed with the thyroids but were not examined. A pathology peer review was conducted by a pathologist designated by the sponsor. Results are reported in Covance 6329-212. 3402 Covance 6329-212 Covance 6329-228 3M T-6316.1 Liver sections from five animals/sex/group from Group 9 were stained with Oil Red "O" and examined microscopically. Statistical Evaluation Levene's test (Levene, 1960) was done to test for variance homogeneity. In the case of heterogeneity of variance at p < 0.05, transformations were used to stabilize the variance. Comparison tests took variance heterogeneity into consideration. One-way analysis of variance [ANOVA (Winer, 1971)] was used to analyze initial body weights, body weight changes, food consumption, continuous clinical pathology values, and organ weight data. ANOVA was done on the homogeneous or transformed data. If the ANOVA was significant, student's t-test was used for pairwise comparisons between treated and control groups. Group comparisons (Group 9 versus Group 8) were evaluated at the 5.0%, two-tailed probability level. Only data collected on or after the first day of treatment were analyzed statistically. Survival and Tumor Analysis Survival was analyzed using life table techniques consisting of Kaplan-Meier product limit estimates, Cox-Tarone binary regression, and Gehan-Breslow nonparametric methods (Thomas, Breslow, and Gart, 1977). Cox-Tarone methods are weighted heavier towards late incidences and Gehan-Breslow methods are weighted towards early incidences due to treatment. As a result, they are both valuable tools for the incidence data with onset times. Week 105 was treated as the end of the study (EOS) in the NCI package for both sexes. The Weeks 4, 14 and 53 interim sacrifices animals and Week 105 terminal sacrifice animals were censored in the analysis. Continuity corrected two-sided tail probability was evaluated at the 5% significance level for comparing the Control vs. Treated group. Nonneoplastic Lesions. Nonneoplastic lesions were chosen for statistical analyses if the incidence rate in the treated group was increased or decreased by at least two occurrences 3403 Covance 6329-212 Covance 6329-228 3M T-6316.1 over the control group. These were analyzed by one-sided Fisher-Irwin exact test at 5% significance level (Thakur, Berry, and Mielke, 1985). Neoplastic Lesions. Neoplastic lesions were chosen for statistical analyses if the incidence rate in the treated group was increased or decreased by at least two occurrences over the control. The incidental tumors (i.e., tumors that were not assigned to be the cause of death of the animals by the study pathologist) were analyzed by logistic regression of tumor prevalence (Dinse and Lagakos, 1983). In this approach, tumor prevalence was modeled as a logistic function of dose and time. Both linear and quadratic terms in time were incorporated initially, and the quadratic term was eliminated if there was lack of convergence. Further, in the cases where there was lack of convergence for the asymptotic test of the logistic regression method, the exact probability of the test was obtained by using LogXact-Turbo (1993). The fatal and palpable (superficial) tumors were analyzed by Cox-Tarone binary regression method as in the case of survival, using the death time or the first palpation time (if applicable) as the tumor onset time. The benign and malignant neoplastic incidences were evaluated separately, as well as combined, where appropriate. The criteria for combination were based on the work of McConnell et al. (1986). The incidences of multiple-organ findings such as fibroma, fibrosarcoma, leiomyoma, leiomyosarcoma, lymphoma, and endometrial stromal polyp were counted by animal, not by tissue type. They were evaluated statistically if they met the selection criterion for the analysis. The statistical results for these cases may be biased because not all the animals were examined for every tissue. One-sided tail probabilities for group comparison was evaluated at the 5% significance level. In the nonneoplastic and neoplastic lesions, early sacrificed animals during or before Week 14 in both groups were excluded from the denominators. Ordinal dose levels 0, and 1 corresponding to Groups 8 (Control), and 9 (Treated) were used in the above statistical analysis. Continuity correction was done for all the asymptotic tests. The following notations are used throughout this report. + = Effect in the increased direction - = Effect in the decreased direction E = Exact permutation test 3404 Covance 6329-212 Covance 6329-228 3M T-6316.1 Record Retention The raw data, documentation, records, specimens, protocol, and final report generated as a result of this study will be archived in the storage facilities of Covance-Madison. At least 1 year after submission of the final report, the Covance Archives staff will contact the Sponsor. At that time, the Sponsor may choose to have the aforementioned materials returned, archived for an additional period of time, or disposed of by Covance; a fee will be charged based on the archive disposition option selected by the Sponsor. Raw data stored on magnetic media and the protocol, study correspondence, and the original final report will be retained by Covance. At least 1 year after submission of the final report, any frozen samples will be returned to the Sponsor, retained by Covance, or disposed of, and a fee will be charged. Data generated by the Sponsor will be archived in the Sponsor's storage facilities. PCNA evaluation data, paraffin blocks, and tissue slides for PCNA will be retained by Pathology Associates International. Frozen liver samples and serum samples sent to the Sponsor will be retained by the Sponsor. RESULTS AND DISCUSSION Dose Analysis Results of the homogeneity, stability, and dose preparation analyses are in Tables 1 through 3. Mean values of the homogeneity analyses for the mix done on March 30, 1998, ranged from 91.3 to 106% of the theoretical concentration for the diet containing 1 ppm N-EtFOSE. These results indicate that the mixing procedure produced a homogeneous distribution of the test material in the dose preparation. Results of the stability analyses for the mix conducted on June 5, 1998, of samples stored 32 days at room temperature conditions indicated that a mean concentration was 81.8% of the theoretical concentration of 1 ppm. Results of stability analyses for the mix conducted on June 5, 1998, of samples stored at room temperature for 46 days indicated that the mean concentration was 98.1% of the theoretical concentration of 1 ppm. The mean concentrations of the dose preparation analyses for the 1-ppm level ranged from 74.3 to 120% of the theoretical concentration (including reassay and retention sample analyses, but excluding data that required reanalysis). Inherent variability and 3405 Covance 6329-212 Covance 6329-228 3M T-6316.1 lack of sufficient sensitivity at low levels in the analytical method for measuring N-EtFOSE resulted in analyses data that were in many cases outside the standardly acceptable limits of +15%. Observation of Animals Survival and Clinical Observations. Clinical observations including mass observations are summarized in Tables 5 and 6; individual data are in Appendix 2. Survival data are illustrated in Figures 1 and 2 and summarized in Table 4. Statistical analyses of survival data are presented in the report of survival analysis. Individual animal fate data are in Appendix 2. Thirty-nine control males and 37 control females and 30 males and 35 females given 1 ppm died on test or were sacrificed in a moribund condition. The most frequent cause of death was pituitary adenomas. There were no significant differences in the mortality in both males and females. Kaplan-Meier estimates indicate there was no treatment-related detrimental effect on the survival of either sex. Clinical observations were similar in nature and in the total number of affected animals in all groups. Body Weights. Body weight and body weight change data are illustrated in Figures 3 and 4 and summarized in Tables 7 and 8; individual body weight data are in Appendix 3. There were no test material-related effects on body weights or body weight changes during the study. Food Consumption. Food consumption data are illustrated in Figures 5 and 6 and summarized in Table 9; individual data are in Appendix 4. There were no test material-related effects on food consumption during the study. Test Material Consumption. Test material consumption data are summarized in Table 10. Individual test material consumption data are presented in Appendix 4. Test material consumption ranged from 0.029 to 0.108 mg/kg/day for Group 9. 3406 Covance 6329-212 Covance 6329-228 3M T-6316.1 Clinical Pathology Hematology, coagulation, clinical chemistry, urinalysis, and urine chemistry data are summarized in Tables 11 through 23 individual data are in Appendix 5. The Pathology Report contains a discussion of the data. Dietary administration of N-EtFOSE at 1 ppm for approximately 52 weeks had no effects on clinical pathology test results. Anatomic Pathology Organ weight data are summarized in Tables 24 through 26; incidences of macroscopic and microscopic observations are summarized in Tables 27 through 36; the incidence of severity of selected microscopic observations is summarized in Tables 37 through 40. Individual data are in Appendix 6. The Pathology Report contains a discussion of the data. There were no test material-related changes for terminal body weights, absolute organ weights, organ-to-body weight percentages or organ-to-brain weight ratios for any of the sacrifices. The incidence of mortality was similar for treated and control animals, and there were no test material-related macroscopic or microscopic findings at any of the study intervals. Proliferation Cell Nuclear Antigen Results The cell proliferation report is in Appendix 7. After 14 weeks on study, enhanced cell proliferation was not evident in the liver of male and female rats exposed to 1 ppm N-EtFOSE. 3407 CONCLUSION Covance 6329-212 Covance 6329-228 3M T-6316.1 Male and female Crl:CD(SD)IGS BR rats were administered 0 or 1 ppm of (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol for at least 104 weeks. There were no test material-related effects on mortality, clinical observations, body weights, food consumption, clinical pathology results, organ weights, macroscopic or microscopic findings. 3408 SIGNATURES Covance 6329-212 Covance 6329-228 3M T-6316.1 3409 Covance 6329-212 Covance 6329-228 3M T-6316.1 REFERENCES Dunnett, C. W., "New Tables for Multiple Comparisons with a Control," Biometrics, 20:482-491 (1964). Levene, H., "Robust Tests for Equality of Variances," Contributions to Probability and Statistics, (eds.) I. Olkin et al., Ch. 25, pp. 278-292, Stanford University Press: Stanford, California (1960). Winer, B. J., "Design and Analysis of Single-Factor Experiments," Statistical Principles in Experimental Design, Second Ed., Ch. 3, pp. 149-260, McGraw-Hill: New York, New York (1971a). Winer, B. J., "Analysis of Covariance," Statistical Principles in Experimental Design, Second Ed., Ch. 10, pp. 752-812, McGraw-Hill: New York, New York (1971b). 3410 PATHOLOGY REPORT Covance 6329-212 Covance 6329-228 3M T-6316.1 SUMMARY The purpose of this study was to assess the carcinogenicity of the test material, N-Ethyl Perfluorooctanesulfonamido Ethanol (N-EtFOSE) when administered in the diet to rats for at least 104 weeks. The test material was administered at a dose level of 1 ppm. Dietary administration of N-EtFOSE at 1 ppm for approximately 52 weeks had no effects on clinical pathology test results. There were no test material-related changes for terminal body weights, absolute organ weights, organ-to-body weight percentages or organ-to-brain weight ratios for any of the sacrifices. The incidence of mortality was similar for treated and control animals, and there were no test material-related macroscopic or microscopic findings at any of the study intervals. METHODS Two groups of male and female Crl:CD(SD)IGS BR rats (70 animals/sex/group) were fed diets containing the test material at a dose level of 0 (control group; received basal diet only) or 1 ppm. These groups were designated as Groups 8 and 9, respectively, and the animals were located in the same animal room as Covance Study No. 6329-212. During Weeks 14, 27, and 53, blood and urine were collected for hematology, clinical chemistry, urinalysis, and urine chemistry tests from 10 animals/sex/group. Blood films were made for all animals sacrificed at unscheduled intervals and at the terminal sacrifice during Week 105. Necropsies were performed on all animals that died or were sacrificed due to poor health, 10 animals/sex/group sacrificed during Week 53, and all remaining surviving animals during Week 105. At necropsy, macroscopic observations were recorded, organ weights were obtained (for sacrifices during Week 53), and tissues were placed in fixative as 3411 Covance 6329-212 Covance 6329-228 3M T-6316.1 specified by the protocol. All tissues from animals in Group 8 and selected tissues from animals in Group 9 were examined microscopically. Five animals/sex/group were sacrificed during Week 4 for hepatic palmitoyl-CoA oxidase activity and during Week 14 for hepatocellular proliferation rate measurements and hepatic palmitoyl-CoA oxidase activity. Statistically significant differences cited in the Results and Discussion section are based on comparisons between the control and treated groups. RESULTS AND DISCUSSION Mortality The incidence of mortality during the study was similar for treated and control animals. Thirty-nine control males and 37 control females, and 30 males and 35 females given 1 ppm died on test or were sacrificed in moribund condition. Clinical Pathology Weeks 4 and 14 (hepatic palmitoyl-CoA oxidase activity). Administration of the test material had no effect on hepatic palmitoyl-CoA oxidase activity. Weeks 14, 27, and 53. There were several small, but statistically significant, differences for clinical pathology results between the control and treated animals. None of these small differences were consistent over time or between sexes, and all were considered to be incidental and unrelated to administration of the test material. Anatomic Pathology/All Animals Macroscopic Findings. The most frequently observed findings at necropsy were, thin animals, ocular discharge, ventral compression of the brain, pituitary masses, and dark foci in the stomach. Other frequent incidental macroscopic findings for males were perineal/perianal staining, rough surface of the kidneys, and dark foci in the stomach. Frequently observed findings for females were mammary masses, ovarian cyst, ocular discharge, and nasal discharge. 3412 Covance 6329-212 Covance 6329-228 3M T-6316.1 Microscopic Findings/Neoplastic Findings. The most frequently observed neoplasm was pituitary adenoma. Islet cell adenomas of the pancreas were frequently present for males, and c-cell adenomas of the thyroid and mammary adenomas, fibroadenomas and carcinomas were frequently present for females. Other tumors were only present in a few animals. Microscopic Findings/Nonneoplastic Findings. The most frequently observed nonneoplastic findings were proteinaceous casts in the kidneys, chronic progressive nephropathy, hepatocellular vacuolation, ventral compression and ventricular dilatation of the brain, degeneration/necrosis of the heart, and pituitary hemorrhage. Frequent microscopic nonneoplastic findings for males were chronic inflammation of the liver, acinar atrophy of the pancreas, fibrosis of the heart, vacuolation of the adrenal cortex, chronic active inflammation of the prostate, and acute inflammation of the footpad. Other frequently observed nonneoplastic microscopic findings for females were pelvic mineralization of the kidneys, ultimobranchial cysts of the thyroid, galactocele, cysts of the thymus, and angiectasis, cystic degeneration, and hemorrhage and thrombi of the adrenal cortex. Anatomic Pathology/Unscheduled Deaths There was no test material-related effect for mortality. The only causes of death observed in three or more animals were hepatocellular adenoma, histiocytic sarcoma, and pituitary adenoma for males, and mammary carcinoma, mammary fibroadenoma, and pituitary adenoma for females. By far the most frequent cause of death was pituitary adenomas. The cause of death was undetermined for 11 males and 2 females. Macroscopic Findings. There were no test material-related macroscopic findings. Microscopic Findings. There were no test material-related microscopic findings for the unscheduled death animals. Anatomic Pathology/Week 4 Sacrifice Organ Weights. There were no test material-related changes for terminal body weights or absolute or relative liver weights. 3413 Covance 6329-212 Covance 6329-228 3M T-6316.1 Macroscopic Findings. There were no macroscopic findings present at necropsy. Anatomic Pathology/Week 14 Sacrifice Organ Weights. There were no test material-related changes for terminal body weights or absolute or relative liver weights. Macroscopic Findings. There were no macroscopic findings present at necropsy. Anatomic Pathology/Week 53 Sacrifice Organ Weights. There were no test material-related changes for terminal body weights or absolute or relative liver weights. Macroscopic Findings. There were no test material-related macroscopic findings. Microscopic Findings. There were no test material-related microscopic findings. The microscopic findings observed at the 53-week sacrifice were typical of those observed as spontaneous findings in rats. Anatomic Pathology/Terminal Sacrifice Macroscopic Findings. There were no test material-related macroscopic findings. Microscopic Findings. There were no test material-related microscopic findings for the terminal sacrifice animals. 3414 Covance 6329-212 Covance 6329-228 3M T-6316.1 STATISTICAL REPORT OF SURVIVAL, NONNEOPLASTIC, AND NEOPLASTIC LESIONS Methods Survival was analyzed using life table techniques consisting of Kaplan-Meier product limit estimates, Cox-Tarone binary regression, and Gehan-Breslow nonparametric methods (Thomas, Breslow, and Gart, 1977). Cox-Tarone methods are weighted heavier towards late incidences and Gehan-Breslow methods are weighted towards early incidences due to treatment. As a result, they are both valuable tools for the incidence data with onset times. Week 105 was treated as the end of the study (EOS) in the National Cancer Institute package for both sexes. The Weeks 4, 14, and 53 interim sacrifices animals and Week 105 terminal sacrifice animals were censored in the analysis. Continuity corrected two-sided tail probability was evaluated at the 5.0% significance level for comparing the Control vs. Treated groups, as reported in Text Tables 1 and 2. Kaplan-Meier product limit survival curves are presented in Figures 1 and 2 for the males and females, respectively. Nonneoplastic Lesions. Nonneoplastic lesions were chosen for statistical analyses if the incidence rate in the treated group was increased or decreased by at least two occurrences over the control group. These were analyzed by one-sided Fisher-Irwin exact test at 5.0% significance level (Thakur, Berry, and Mielke, 1985), and the results are reported in Text Tables 3 and 4 for the males and females, respectively. Neoplastic Lesions. Neoplastic lesions were chosen for statistical analyses if the incidence rate in the treated group was increased or decreased by at least two occurrences over the control. The incidental tumors (i.e., tumors that were not assigned to be the cause of death of the animals by the study pathologist) were analyzed by logistic regression of tumor prevalence (Dinse and Lagakos, 1983). In this approach, tumor prevalence was modeled as a logistic function of dose and time. Both linear and quadratic terms in time were incorporated initially, and the quadratic term was eliminated if there was lack of convergence. Further, in the cases where there was lack of convergence for the asymptotic test of the logistic regression method, the exact probability of the test was obtained by using LogXact-Turbo (1993). The fatal and palpable (superficial) tumors were analyzed by Cox-Tarone binary regression method, as in the case of survival, using the death time or the first palpation time (if applicable) as the tumor onset time. 3415 Covance 6329-212 Covance 6329-228 3M T-6316.1 The benign and malignant neoplastic incidences were evaluated separately as well as combined where appropriate. The criteria for combination were based on the work of McConnell et al. (1986). The incidences of multiple-organ findings such as fibroma, fibrosarcoma, leiomyoma, leiomyosarcoma, lymphoma, and endometrial stromal polyp were counted by animal, not by tissue type. They were evaluated statistically if they met the selection criterion for the analysis. The statistical results for these cases may be biased because not all the animals were examined for every tissue. One-sided tail probabilities for group comparison were evaluated at the 5.0% significance level and are shown in Text Tables 5 and 6 for the males and females, respectively. In the nonneoplastic analyses, animals sacrificed early (Week 4) were excluded. In the statistical evaluation of the neoplastic lesions, animals sacrificed early (during or before Week 14) were discounted from the number of animals at risk. Ordinal dose levels 0 and 1 corresponding to Groups 8 (Control) and 9 (Treated) were used in the above statistical analysis. Continuity correction was done for all the asymptotic tests. The following notations are used throughout this report. + = Effect in the increased direction - = Effect in the decreased direction E = Exact permutation test Results and Discussion Survival. As indicated by the Kaplan-Meier adjusted survival curves (Figures 1 and 2) and by Text Tables 1 and 2, there was no significant difference in mortality in either the males or females. Nonneoplastic Lesions. The liver in both sexes has been identified as the only organ that is appropriate for performing statistical analyses for nonneoplastic lesions. As shown in Text Tables 3 and 4, no significant differences were noted in any of the hepatocellular nonneoplastic lesions in either sex. Neoplastic Lesions. As indicated in Text Tables 5 and 6, there were no significant incidences of any neoplastic findings in either sex. 3416 Covance 6329-212 Covance 6329-228 3M T-6316.1 References Dinse, G.E. and Lagakos, S.W., "Regression analysis of tumor prevalence data," Journal of Royal Statistical Society Series C (Applied Statistics), 32: 236-248 (1983). Gart, J.J., Krewski, D., Lee, P.N., Tarone, R.E., and Wahrendorf, J., "Statistical Methods in Cancer Research," Vol. III, The Design and Analysis of Long-Term Animal Experiments, IARC Scientific Publications No. 79, Oxford University Press (1986). LogXact-Turbo, Cytel Software Corporation, Version 1.1, Cambridge, Massachusetts (1993). McConnell, E.E., Solleveld, H.A., Swenberg, J.A., and Boorman, G.A., "Guidelines for combining neoplasms for evaluation of rodent carcinogenicity studies," Journal of National Cancer Institute, 76:283-289 (1986). Peto, R., Pike, M. C., Day, N. E., Gray, R. G., Lee, P. N., Parish, S., Peto, J., Richards, S., and Wahrendorf, J., "Guidelines for Simple Sensitive Significance Tests for Carcinogenic Effects in Long-term Animal Experiments, in Long-term and Short-term Screening Assays for Carcinogens: A Critical Appraisal," Lyon: International Agency for Cancer Research, pp. 311-426 (1980). Thakur, A.K., Berry, K.J., and Mielke, P.W., Jr., "A FORTRAN program for testing trend and homogeneity in proportions," Computer Programs in Biomedicine, 19: 229-233 (1985). Thomas, D.G., Breslow, N., and Gart, J. J., "Trend and homogeneity analyses of proportions and life table data," Computers and Biomedical Research, 10:373-381 (1977). 3417 Covance 6329-212 Covance 6329-228 3M T-6316.1 Text Table 1 Results of Statistical Analyses of Survival Data for Male Rats Group Unadjusted Mortality Adjusted Mortality: Kaplan-Meier Estimate (Final) Standard Error 8 (Control) 39/70 (.557) .778 .059 9 (Treated) 30/70 (.429) .597 .069 Groups 8 vs 9 p (two-sided) - = Effect in the decreased direction. Cox-Tarone Test .0818 - Gehan-Breslow Test .1426 - Text Table 2 Results of Statistical Analyses of Survival Data for Female Rats Group Unadjusted Mortality Adjusted Mortality: Kaplan-Meier Estimate (Final) Standard Error 8 (Control) 37/70 (.529) .736 .063 9 (Treated) 35/70 (.500) .698 .065 Groups 8 vs 9 p (two-sided) - = Effect in the decreased direction. Cox-Tarone Test .4492 - Gehan-Breslow Test .2491 - 3418 Covance 6329-212 Covance 6329-228 3M T-6316.1 Text Table 3 Results of Statistical Analyses of Nonneoplastic Lesions in the Males Group LIVER - HEMORRHAGE One-sided p-value 8 (Control) 5/65 9 (Treated) 2/65 .22005 - LIVER - INFLAMMATION, CHRONIC 17/65 One-sided p-value 15/65 .41946 - LIVER - VACUOLATION, HEPATOCELLULAR 14/65 One-sided p-value 10/65 .24915 - LIVER - DEGENERATION, CYSTIC 5/65 One-sided p-value 9/65 .19845 + LIVER - FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC 4/65 9/65 One-sided p-value .12081 + LIVER - CYST 2/65 One-sided p-value 0/65 .24806 - 3419 Covance 6329-212 Covance 6329-228 3M T-6316.1 Text Table 4 Results of Statistical Analyses of Nonneoplastic Lesions in the Females Group LIVER - CYST, BILIARY One-sided p-value 8 (Control) 2/65 9 (Treated) 0/65 .24806 - LIVER - INFLAMMATION, CHRONIC 8/65 One-sided p-value 6/65 .38915 - LIVER - VACUOLATION, HEPATOCELLULAR 14/65 One-sided p-value 8/65 .12091 - LIVER - HYPERPLASIA BILE DUCT 5/65 One-sided p-value 7/65 .38163 + LIVER - FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC 1/65 3/65 One-sided p-value .30956 + LIVER - FOCUS, ALTERED HEPATOCELLULAR, EOSINPHILIC 3/65 7/65 One-sided p-value .16203 + LIVER - TELANGIECTASIS 5/65 One-sided p-value 2/65 .22005 - 3420 Covance 6329-212 Covance 6329-228 3M T-6316.1 Text Table 5 Results of Statistical Analyses of Neoplastic Lesions in the Male Rats Group Pancreas - Islet Cell, Adenoma Fatal Incidence Incidental Incidence Total Incidence Rate 8 (Control) 0 4 4/60 9 (Treated) 0 11 11/60 One-sided p-value .0825 + Testis - Interstitial Cell, Adenoma Fatal Incidence 0 0 Incidental Incidence 0 3 Total Incidence Rate 0/60 3/55 One-sided p-value .2536 +(E) 3421 Covance 6329-212 Covance 6329-228 3M T-6316.1 Text Table 6 Results of Statistical Analyses of Neoplastic Lesions in the Female Rats Group Pancreas - Islet Cell, Adenoma Fatal Incidence Incidental Incidence Total Incidence Rate 8 (Control) 0 4 4/59 9 (Treated) 0 1 1/60 One-sided p-value .1200 - Mammary - Adenoma Total Incidence Rate 5/60 One-sided p-value 2/60 .1791 - Mammary - Fibroadenoma Total Incidence Rate 21/60 22/60 Note: Incidences across groups do not meet the selection criterion. Mammary - Adenoma/Fibroadenoma Total Incidence Rate 25/60 One-sided p-value 23/60 .1929 - Mammary - Carcinoma Total Incidence Rate 9/60 9/60 Note: Incidences across groups do not meet the selection criterion. Mammary - Adenoma/Fibroadenoma/Carcinoma Total Incidence Rate 29/60 One-sided p-value 27/60 .1877 - 3422 Covance 6329-212 Covance 6329-228 3M T-6316.1 Figure 1 - Kaplan-Meier Survival Curves For Male Rats 0 15 30 45 60 75 90 105 + + + + + + + + ----------------------------------------------------------------------- 1.0+********111111111111111111111 + 000000000000000000000*000000 + 111111*******000 + 1110 + 1000 .9+ 11 0 P+ 11*0 R+ 100 O+ 11*11 P+ 001 O .8+ 0*00 R+ 110 T+ 1* I+ *11 O+ 00*1111 N .7+ 000 11 + 0 11 S+ 00 11 U+ 00 111 R+ 0 111 V .6+ 0 1 I+ 0 11 V+ 00 1 I+ 0 1 N+ 000 111 G .5+ 00 1 + 0 1 + 00 111 + 0 1 + 00 1 .4+ 000 11 + 00 + 00 + 0 + 0 .3+ 00 + 00 + 0 + 0 + 00 .2+ + + 0 = Group 8 (Control) + 1 = Group 9 (Treated) + * = More Than One Data Point .1+ + + + + .0+ ----------------------------------------------------------------------- + + + + + + + + 0 15 30 45 60 75 90 105 SURVIVAL TIME (Week) 3423 Covance 6329-212 Covance 6329-228 3M T-6316.1 Figure 2 - Kaplan-Meier Survival Curves For Female Rats 0 15 30 45 60 75 90 105 + + + + + + + + ----------------------------------------------------------------------- 1.0+******1111111111111111111111111111 + 0000000000000000 11 + 0000000000000011111111 + 000000 1 + 01 .9+ 00 11 P+ 000* R+ *111 O+ 00 1 P+ 00**1 O .8+ 0*11 R+ 00111 T+ 00 111 I+ 0 11 O+ 0000 1 N .7+ 0011 + 00111 S+ 00 111 U+ 01 R+ 0 111 V .6+ 0 1 I+ 000 1 V+ 00 111 I+ 00 11 N+ 01 G .5+ 000 11 + 00 1 + 0 11 + 00 1 + 0 11 .4+ 01 + 00 1 + 0 11 + 000011 + 01 .3+ 0011 + 00 + 00 + + 0 = Group 8 (Control) .2+ 1 = Group 9 (Treated) + * = More Than One Data Point + + + .1+ + + + + .0+ ----------------------------------------------------------------------- + + + + + + + + 0 15 30 45 60 75 90 105 SURVIVAL TIME (Week) 3424 Covance 6329-212 Covance 6329-228 3M T-6316.1 COMMENTS ON THE DATA Various models of calculators, computers, and computer programs were used to analyze data in this study. Because different models round off or truncate numbers differently, values in some tables (e.g., means, standard deviations, or individual values) may differ slightly from those in other tables, from individually calculated data, or from statistical analysis data. Neither the integrity nor the interpretation of the data was affected by these differences. The units for the dose levels in the inlife summary tables are ppm. The number of animals listed in the heading of the summary tables for clinical and mass observations reflects the number of animals assigned to each group at the start of the study. The summary tables for clinical observations indicates the number of animals for which a condition was observed without regard to the reversibility, number of incidences/animal, or the length of time the condition persisted. Only observations for each animal other than normal are indicated in the summary clinical observations tables. Each animal with observations recorded as "Normal" throughout the study has the comment "No remarkable observations" indicated in the individual clinical observations tables. The specific details for comments in the individual clinical observations tables that are indicated with a "C" can be found at the end of each group for each sex. Food consumption values are reported in whole numbers; however, the data collection system carries food consumption values to one place to the right of the decimal for test material consumption calculations. The differences in values generated do not influence the interpretation of the calculation. The comment "SPILLED" in individual food consumption data tables indicates that food consumption was not recorded due to spillage during the interval. The comment "NOT TAKEN" in individual food consumption data tables indicates that the animal died before the end of the food consumption interval. Differences in the population size (N) in the summary tables for clinical and anatomic pathology are explained in the individual data tables or the codes sheets or in the Protocol Deviations section. 3425 Covance 6329-212 Covance 6329-228 3M T-6316.1 COMMENTS ON THE DATA (Continued) Results of clinical pathology samples collected for animals sacrificed at unscheduled intervals appear in the individual clinical pathology tables for the next scheduled interval. These results are not in the summary tables and are not included in the statistical analyses. Statistical analyses were not done when there were two or fewer values for a parameter. The calculation for individual test material consumption is: Test material consumption = Diet ConcentrationxFood Consumption Food Consumption Intervalx[First Body Weight of Interval+ (Daily Body Weight GainxDay Factor)] where: Daily Body Weight Gain = Last Body Weight of Interval- First Body Weight of Interval Day of Last Body Weight of Interval- Day of First Body Weight of Interval and: Day Factor = (Food Consumption Start Day- Day of First Body Weight of Interval) + Food Consumption Interval 2 and: Food Consumption Interval = Food Consumption End Day- Food Consumption Start Day 3426 CODES, ABBREVIATIONS, AND UNITS Covance 6329-212 Covance 6329-228 3M T-6316.1 General Codes and Abbreviations Codes for Clinical Pathology Abbreviations and Units for Clinical Hematology Abbreviations and Units for Clinical Chemistry Abbreviations and Units for Clinical Urinalysis Abbreviations and Units for Urine Chemistry Codes for Anatomic Pathology Note: The following lists of codes, abbreviations, and units are used by Covance. Some, but not necessarily all, of this information may be needed for this report. 3427 Covance 6329-212 Covance 6329-228 3M T-6316.1 General Codes and Abbreviations WK N Mean; MEAN CAM SD; S.D.; STAND DEV; STANDARD DEV; sd * -; NA P C UNSCHED DISPATCH Week Number of measurements in a group Arithmetic mean Covariate-adjusted mean Standard deviation Group mean is significantly different from the mean of the control group (Group 1) at p < 0.05 No value; not applicable; not present Present Comment found at the end of each group for each sex Unscheduled Observations transferred from the inlife module of the data collection system to the necropsy module for reference during necropsy. Observations are duplicates of the last inlife observations. Animal Death Codes 1 2 3 T D M Interim sacrifice, Week 4 Interim sacrifice, Week 14 Interim sacrifice, Week 52 Terminal sacrifice Found dead Moribund 3428 NS QS/QNS NR FS SC SH H SL L SI I NF U DT/DOT DB TJ TE RE EE SE PC PD PI PL PA CO HB PLASMO NO AGG FR UTD NO COAG Covance 6329-212 Covance 6329-228 3M T-6316.1 Codes for Clinical Pathology GENERAL CODES No sample Quantity not sufficient No repeat (sample volume not sufficient for repeat analysis) Fibrin strands Sample clotted Slightly hemolyzed Hemolyzed Slightly lipemic Lipemic Slightly icteric Icteric Animal not fasted Unscheduled/moribund bleed Animal died on test Died during bleeding Technician judgment to repeat test Technical error (instrument or technician error that results in unacceptable data, e.g., unacceptable instrument output, sample spilled, entry of invalid data) Recording error (recorded incorrect data, e.g., wrong number, spelling error, incorrect date) Entry error (incorrect keyboard entry) Sampling error Platelets clumped Platelets decreased Platelets increased Platelets large Platelets appear adequate Color interferes with test Heinz bodies observed Plasmodium No aggregation Fractious Unable to determine No coagulation 3429 Covance 6329-212 Covance 6329-228 3M T-6316.1 Codes for Clinical Pathology (Continued) RESULTS NOT INCLUDED IN STATISTICAL ANALYSES Hemolyzed clinical chemistry or coagulation samples Samples from animals at unscheduled intervals Prothrombin times (PT) greater than 50 seconds Activated partial thromboplastin times (PTT) greater than 110 seconds Bleed times (BLETIME) greater than 30 minutes CODES FOR BLOOD CELL MORPHOLOGY The following scale was used to measure the degree of anisocytosis (ANISO), poikilocytosis (POIK), polychromasia (POLY), hypochromasia (HYPO), or basophilic stippling (BASTIP) or the presence of Howell-Jolly bodies (HJBODY), toxic neutrophils (TOXNEUT), or atypical lymphocytes (ATYPLYM). Scale 1 2 3 4 Degree Normal for the species Slight Moderate Marked Not applicable Presence Not present Rare Few Moderate Many URINE APPEARANCE A Pale B Straw C Yellow D Dark yellow Color E Amber F Brown G Red H Green I Black P Blue/green Q Blue R Orange Clarity J Clear K Hazy L Cloudy Miscellaneous M Debris O Feces 3430 Covance 6329-212 Covance 6329-228 3M T-6316.1 Codes for Clinical Pathology (Continued) URINE CHEMISTRY MULTISTIX STRIP Urine Glucose + ++ +++ ++++ +++++ Negative 100 mg/dL 250 mg/dL 500 mg/dL 1,000 mg/dL >2,000 mg/dL Urine Ketone + ++ +++ ++++ +++++ Negative 5 mg/dL 15 mg/dL 40 mg/dL 80 mg/dL 160 mg/dL Urine Blood - Negative + Small ++ Moderate +++ Large Urine Urobilinogen + ++ +++ ++++ 0.2 mg/dL 1 mg/dL 2 mg/dL 4 mg/dL 8 mg/dL (1 mg = approximately 1 Ehrlich unit) Urine Bilirubin - Negative + Small ++ Moderate +++ Large URINE SEDIMENT Cells, Crystals, Casts, and Comments A Amorphous urates B Amorphous phosphates C Uric acid D Triple phosphates E Calcium oxalate F Calcium carbonate G Granular casts H Hyaline casts I Cellular casts J Waxy casts K Unknown crystal P Mucous threads Q Sperm R Fecal contamination S Pinworm ova found T Pinworm larvae found U Parasite ova found 0 Not present 1 1 - 5 per field 2 6 - 10 per field 3 11 - 20 per field 4 >20 per field Bacteria 0 Not present 1 Few 2 Moderate 3 Many 3431 Covance 6329-212 Covance 6329-228 3M T-6316.1 Abbreviations and Units for Clinical Hematology Test Red blood cell count Hemoglobin Hematocrit Mean corpuscular volume Mean corpuscular hemoglobin Mean corpuscular hemoglobin concentration Platelet count White blood cell count Differential blood cell count Segmented neutrophil count Lymphocyte count Monocyte count Eosinophil count Basophil count Anisocytosis Polychromasia Poikilocytosis Hypochromasia Toxic neutrophils Abbreviation (Units) RBC (E6/UL or X106/.L) HGB (G/DL) HCT (%) MCV (FL) MCH (PG) MCHC (%) PLT (E3/UL or X103/.L) WBC (E3/UL or X103/.L) N-SEG (E3/UL or X103/.L) and % LYMPH (E3/UL or X103/.L) and % MONO (E3/UL or X103/.L) and % EOSIN (E3/UL or X103/.L) and % BASO (E3/UL or X103/.L) and % ANISO (-,1,2,3) POLY (-,1,2,3) POIK (-,1,2,3) HYPO (-,1, 2,3) TOXNEUT (-,1,2,3,4) 3432 Covance 6329-212 Covance 6329-228 3M T-6316.1 Abbreviations and Units for Clinical Chemistry Test Glucose Urea nitrogen Creatinine Total protein Albumin Globulin Total bilirubin Cholesterol Triglyceride Aspartate aminotransferase Alanine aminotransferase Gamma glutamyltransferase Palmitoyl CoA oxidase Calcium Inorganic phosphorus Sodium Potassium Chloride Abbreviation (Units) GLU (MG/DL) UN (MG/DL) CREAT (MG/DL) T PRO (G/DL) ALB (G/DL) GLOB (G/DL) T BILI (MG/DL) CHOL (MG/DL) TRIG (MG/DL) AST/SGOT (IU/L) ALT/SGPT (IU/L) GGT (IU/L) PCOAO (IU/G) CA (MG/DL) I PHOS (MG/DL) NA (MMOL/L) K (MMOL/L) CL (MMOL/L) 3433 Covance 6329-212 Covance 6329-228 3M T-6316.1 Abbreviations and Units for Clinical Urinalysis Test Urine volume Specific gravity Urine chemistry Multistix strip Urine pH Urine protein Urine glucose Urine ketones Urine bilirubin Urine blood Urine urobilinogen Microscopic examination of urine sediment Red blood cells per high-power field White blood cells per high-power field Epithelial cells per high-power field Bacteria per high-power field Casts per low-power field Crystals per low-power field Urine appearance Comments Abbreviation (Units) U VOL (ML) SP GR U PH U PRO (MG/DL) U GLU U KET U BILI U BLOOD UROBILI RBC (PER HPF) WBC (PER HPF) EPITH (PER HPF) BACT (PER HPF) CASTS (PER LPF) CRYSTALS (PER LPF1 or PER LPF2) URINE APP1 or URINE APP2 COMMENTS Abbreviations and Units for Urine Chemistry Test Urine sodium Urine potassium Sodium excretion Potassium excretion Abbreviation (Units) U NA (MMOL/L) U K (MMOL/L) NA EXC (MMOL) K EXC (MMOL) 3434 Code EX NOT TAKEN MISSING UNSUITABLE AUTOLYTIC EXCLUDE Covance 6329-212 Covance 6329-228 3M T-6316.1 Codes for Anatomic Pathology Definition MACROSCOPIC CODES Indicates that organ weight is excluded from calculations Organ weight not taken; explanation given in necropsy notes Organ missing or lost Organ technically unsuitable for weighing Organ autolyzed and could not be weighed Weight was taken, but was excluded from all calculations MICROSCOPIC CODES Codes Prefacing Neoplastic Findings B- Primary, benign neoplasm M- Primary, malignant neoplasm N- Metastatic neoplasm I- Locally invasive neoplasm X- Other neoplasm Distribution of Findings Focal Diffuse Multifocal Grades for Severity or Amount 1 Minimal - the least amount of change that can be observed with the light microscope 2 Slight - less than average amount of change, but readily discernible as abnormal 3 Moderate - the average amount of change that is expected for a lesion 4 Moderately severe (marked) - a marked amount of change with possible loss of function of the affected cells or organs 5 Severe - a great amount of change with probable loss of function of the affected cell or organs and frequently involves large areas of the organ 3435 Codes for Anatomic Pathology (Continued) Other Microscopic Codes TL Total P Finding present - Finding not present MN Mean Covance 6329-212 Covance 6329-228 3M T-6316.1 TISSUE ABBREVIATIONS Abbreviation LN GL STOMACH, GL STOMACH, NONGL SALIV GL, MANDIB LN, ANT MES/PANC AUDITORY SEB GL LACRIMAL GLAND, EX HEMATO NEOPLASIA LACRIMAL GL, INT CAVITY, ABDOM SALIV GL,PAROTID LN, TRACHEOBRON Definition Lymph node Gland Glandular stomach Nonglandular stomach Mandibular salivary gland Anterior mesenteric/pancreatic lymph node Auditory sebaceous gland Exorbital lacrimal gland Hematopoietic neoplasia Internal lacrimal gland Abdominal cavity Parotid salivary gland Tracheobronchial lymph node 3436 Covance 6329-212 (%) Figure 1 Adjusted Survival Data (%) - Males Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO IN RATS 100 90 80 70 60 50 40 30 0 ppm 1 ppm 20 10 0 0 4 8 12 16 20 24 28 32 36 40 44 48 52 56 60 64 68 72 76 80 84 88 92 96 100 104 Week 3437 Covance 6329-212 (%) Figure 2 Adjusted Survival Data (%) - Females Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO IN RATS 100 90 80 70 60 50 40 0 ppm 1 ppm 30 20 10 0 0 4 8 12 16 20 24 28 32 36 40 44 48 52 56 60 64 68 72 76 80 84 88 92 96 100 104 Week 3438 Covance 6329-212 (g) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 21 25 29 33 37 41 45 49 53 57 61 65 69 73 77 81 85 89 93 97 101 105 Figure 3 Mean Body Weight Data (g) - Males Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) 850 N-ETHYL PERFLUOROOCTANESULFONAMIDO IN RATS 800 750 700 650 600 550 500 450 400 350 300 0 ppm 250 1 ppm 200 150 100 50 0 Week 3439 Covance 6329-212 (g) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 21 25 29 33 37 41 45 49 53 57 61 65 69 73 77 81 85 89 93 97 101 105 Figure 4 Mean Body Weight Data (g) - Females Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) 600 N-ETHYL PERFLUOROOCTANESULFONAMIDO IN RATS 550 500 450 400 350 300 250 0 ppm 200 1 ppm 150 100 50 0 Week 3440 Covance 6329-212 Figure 5 Mean Food Consumption Data (g) - Males 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO IN RATS Covance 6329-228 3M T-6316.1 225 200 175 150 125 0 ppm 1 ppm 100 75 1 3 5 7 9 11 13 15 20 28 36 44 52 60 68 76 84 92 100 106 Week (g) 3441 Covance 6329-212 Figure 6 Mean Food Consumption Data (g) - Females 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO IN RATS Covance 6329-228 3M T-6316.1 200 175 150 (g) 125 0 ppm 1 ppm 100 75 1 3 5 7 9 11 13 15 20 28 36 44 52 60 68 76 84 92 100 106 Week 3442 Covance 6329-212 Covance 6329-228 3M T-6316.1 Table 1 Results of Homogeneity Analyses (ppm) Mixed 3/30/98 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS T-6316 (ppm) Sample Location Replicate 1 Top 1 0.824 2 1.01 3 Mean 0.906 0.913 (91.3)a Middle 1 2 3 Mean 1.02 0.894 0.900 0.938 (93.8) Bottom 1 2 3 Mean 0.766 1.28 1.13 1.06 (106) a Each value in parentheses is the percent of theoretical. 3443 Covance 6329-212 Covance 6329-228 3M T-6316.1 Table 2 Results of Stability Analyses (ppm) Mixed 3/30/98 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Interval Initiala T-6316 (ppm) Replicate 1 1 2 3 Mean 1.02 0.894 0.900 0.938 (93.8)b 21 days at room temperature 1 2 Mean 0.892 0.842 0.867 (86.7) 32 days at room temperature 1 2 Mean 1.39 1.20 1.30 (130) a Results were derived from the sample collected from the middle of the dose preparations for homogeneity analysis. b Each value in parentheses is the percent of theoretical. 3444 Covance 6329-212 Covance 6329-228 3M T-6316.1 Table 2 (Continued) Results of Stability Analyses (ppm) Mixed 6/5/98 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Interval T-6316 (ppm) Replicate 1 Initial 1 2 Mean 0.966 1.13 1.05 (105)b 32 days at room temperature 1 2 Mean 0.828 0.808 0.818 (81.8) 46 days at room temperature 1 2 Mean 1.06 0.902 0.981 (98.1) b Each value in parentheses is the percent of theoretical. 3445 Covance 6329-212 Covance 6329-228 3M T-6316.1 Table 3 Results of Dose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Week 1a T-6316 (ppm) Replicate 0 1 1 b 2 b 3 - Mean - 1.02 0.894 0.900 0.938 (93.8)c 2-5 1 b 0.782 2 b 0.668 Mean - 0.725 (72.5) 2-5d 1 - 1.12 2 - 1.04 Mean - 1.08 (108) 6-9 1 0.848 1.48 2 b 1.53 Mean - 1.51 (151) 6-9d 1 b 0.890 2 b 0.858 Mean - 0.874 (87.4) a Results for the 1 ppm level were derived from the sample collected from the middle of the dose preparations for homogeneity analysis. b Below the limit of quantitation (<0.5 ppm). c Each value in parentheses is the percent of the theoretical. d Reassay. 3446 Covance 6329-212 Covance 6329-228 3M T-6316.1 Table 3 (Continued) Results of Dose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Week T-6316 (ppm) Replicate 0 1 10-13 1 b 2 b Mean - 0.966 1.13 1.05 (105)c 14-17 1 b 2 b Mean - 0.894 0.870 0.882 (88.2) 18-21 18-21d 18-21e 1 b 2 b Mean - 1 - 2 - Mean - 1 - 2 - Mean - 1.69 1.07 1.38 (138) 0.880 1.26 1.07 (107) 0.789 0.704 0.747 (74.7) 22-25 1 b 2 b Mean - 0.798 0.876 0.837 (83.7) b Below the limit of quantitation (<0.5 ppm). c Each value in parentheses is the percent of the theoretical. d Reassay. e Retention. 3447 Covance 6329-212 Covance 6329-228 3M T-6316.1 Table 3 (Continued) Results of Dose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Week T-6316 (ppm) Replicate 0 1 26-29 26-29d 26-29e 1 b 2 b Mean - 1 - 2 - Mean - 1 - 2 - Mean - 8.81 1.88 5.35 (535)c 0.730 0.786 0.758 (75.8) 0.902 0.936 0.919 (91.9) 30-33 30-33d 1 b 2 b Mean - 1 - 2 - Mean - 1.05 1.32 1.19 (119) 0.951 0.955 0.953 (95.3) 34-37 1 b 2 b Mean - 1.02 1.00 101 (101) b Below the limit of quantitation (<0.5 ppm). c Each value in parentheses is the percent of the theoretical. d Reassay. e Retention. 3448 Covance 6329-212 Covance 6329-228 3M T-6316.1 Table 3 (Continued) Results of Dose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Week T-6316 (ppm) Replicate 0 1 38-41 1 b 2 b Mean - 0.836 1.16 0.998 (99.8)c 42-45 1 b 2 b Mean - 0.912 0.886 0.899 (89.9) 46-49 46-49d 46-49e 1 b 2 b Mean - 1 - 2 - Mean - 1 - 2 - Mean - 0.781 0.705 0.743 (74.3) 0.648 0.669 0.659 (65.9) 0.568 0.573 0.571 (57.1) 50-53 1 b 2 b Mean - 0.895 2.18 1.54 (154) b Below the limit of quantitation (<0.5 ppm). c Each value in parentheses is the percent of the theoretical. d Reassay. e Retention. 3449 Covance 6329-212 Covance 6329-228 3M T-6316.1 Table 3 (Continued) Results of Dose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Week 50-53d T-6316 (ppm) Replicate 0 1 1 - 2 - Mean - 0.916 0.904 0.910 (91.0)c 54-57 54/57d 54/57e 1 b 2 b Mean - 1 - 2 - Mean - 1 - 2 - Mean - 0.977 0.784 0.881 (88.1) 0.775 0.742 0.759 (75.9) 0.444 0.501 0.473 (47.3) 58-61 1 b 2 b Mean - 1.09 0.945 1.02 (102) 58-61d 1 - 2 - Mean - 0.782 0.987 0.885 (88.5) 58-61e 1 - 1.05 2 - 0.938 Mean - 0.994 (99.4) b Below the limit of quantitation (<0.5 ppm). c Each value in parentheses is the percent of the theoretical. d Reassay. e Retention. 3450 Covance 6329-212 Covance 6329-228 3M T-6316.1 Table 3 (Continued) Results of Dose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Week T-6316 (ppm) Replicate 0 1 62-65 62-65d 62-65e 1 b 2 b Mean - 1 - 2 - Mean - 1 - 2 - Mean - 0.813 1.18 0.997 (99.7)c 0.727 0.679 0.703 (70.3) 0.716 0.872 0.794 (79.4) 66-69 66-69d 66-69e 1 b 2 b Mean - 1 - 2 - Mean - 1 - 2 - Mean - 0.700 0.786 0.743 (74.3) 0.969 0.763 0.866 (86.6) 0.549 0.490 0.520 (52.0) b Below the limit of quantitation (<0.5 ppm). c Each value in parentheses is the percent of the theoretical. d Reassay. e Retention. 3451 Covance 6329-212 Covance 6329-228 3M T-6316.1 Table 3 (Continued) Results of Dose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Week T-6316 (ppm) Replicate 0 1 70-73 70-73d 1 b 2 b Mean - 1 - 2 - Mean - 0.889 0.780 0.835 (83.5)c 0.498 0.478 0.488 (48.8) 74-77 74-77d 1 b 2 b Mean - 1 - 2 - Mean - 1.22 1.17 1.20 (120) 0.592 0.806 0.699 (69.9) b Below the limit of quantitation (<0.5 ppm). c Each value in parentheses is the percent of the theoretical. d Reassay. 3452 Covance 6329-212 Covance 6329-228 3M T-6316.1 Table 3 (Continued) Results of Dose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Week T-6316 (ppm) Replicate 0 1 78-81 1 b 2 b Mean - 0.856 1.01 0.933 (93.3)c 82-85 1 b 2 b Mean - 0.582 0.635 0.609 (60.9) 86-89 1 b 2 b Mean - 0.970 1.13 1.05 (105) 90-93 90-93d 1 b 2 b Mean - 1 - 2 - Mean - 0.765 0.886 0.826 (82.6) 3.22 3.19 3.21 (321) 94-97 94-97d 1 b 2 b Mean - 1 - 2 - Mean - 0.756 0.919 0.838 (83.8) 0.677 0.683 0.680 (68.0) b Below the limit of quantitation (<0.5 ppm). c Each value in parentheses is the percent of the theoretical. d Reassay. 3453 Covance 6329-212 Covance 6329-228 3M T-6316.1 Table 3 (Continued) Results of Dose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Week 94-97e Replicate 0 1 - 2 - Mean - T-6316 (ppm) 1 0.658 0.627 0.643 (64.3) 98-101 1 b 2 b Mean - 0.806 0.923 0.865 (86.5) 102-104 1 b 2 b Mean - 1.16 1.07 1.12 (112) b Below the limit of quantitation (<0.5 ppm). c Each value in parentheses is the percent of the theoretical. e Retention. 3454 Covance 6329-212 Table 4 Survival Data Covance 6329-228 3M T-6316.1 PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: START 1 2 3 4 5 6 7 8 9 10 11 ---------------------------------------------------------------------------------------------------------------------- MALE 8 0 70/70 70/70 70/70 70/70 65/65 65/65 65/65 65/65 65/65 65/65 65/65 64/65 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 98% 9 1 70/70 70/70 70/70 70/70 65/65 65/65 65/65 65/65 65/65 65/65 65/65 65/65 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% FEMALE 8 0 70/70 70/70 70/70 70/70 65/65 65/65 65/65 65/65 64/65 64/65 64/65 64/65 100% 100% 100% 100% 100% 100% 100% 100% 98% 98% 98% 98% 9 1 70/70 70/70 70/70 70/70 65/65 65/65 65/65 65/65 65/65 65/65 65/65 65/65 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 3455 Covance 6329-212 Table 4 Survival Data Covance 6329-228 3M T-6316.1 PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 12 13 14 15 16 17 18 19 20 21 22 23 ---------------------------------------------------------------------------------------------------------------------- MALE 8 0 64/65 64/65 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 9 1 65/65 65/65 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% FEMALE 8 0 64/65 64/65 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 9 1 65/65 65/65 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 3456 Covance 6329-212 Table 4 Survival Data Covance 6329-228 3M T-6316.1 PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 24 25 26 27 28 29 30 31 32 33 34 35 ---------------------------------------------------------------------------------------------------------------------- MALE 8 0 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 9 1 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% FEMALE 8 0 59/60 59/60 59/60 59/60 59/60 59/60 58/60 58/60 58/60 58/60 58/60 58/60 98% 98% 98% 98% 98% 98% 97% 97% 97% 97% 97% 97% 9 1 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 3457 Covance 6329-212 Table 4 Survival Data Covance 6329-228 3M T-6316.1 PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 36 37 38 39 40 41 42 43 44 45 46 47 ---------------------------------------------------------------------------------------------------------------------- MALE 8 0 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 98% 9 1 60/60 60/60 60/60 60/60 60/60 60/60 58/60 58/60 58/60 58/60 58/60 58/60 100% 100% 100% 100% 100% 100% 97% 97% 97% 97% 97% 97% FEMALE 8 0 58/60 58/60 58/60 58/60 58/60 58/60 58/60 58/60 58/60 58/60 58/60 58/60 97% 97% 97% 97% 97% 97% 97% 97% 97% 97% 97% 97% 9 1 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 3458 Covance 6329-212 Table 4 Survival Data Covance 6329-228 3M T-6316.1 PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 48 49 50 51 52 53 54 55 56 57 58 59 ---------------------------------------------------------------------------------------------------------------------- MALE 8 0 59/60 59/60 59/60 58/60 58/60 48/50 48/50 48/50 48/50 48/50 48/50 48/50 98% 98% 98% 97% 97% 96% 96% 96% 96% 96% 96% 96% 9 1 58/60 58/60 58/60 58/60 58/60 48/50 48/50 48/50 48/50 48/50 48/50 48/50 97% 97% 97% 97% 97% 96% 96% 96% 96% 96% 96% 96% FEMALE 8 0 57/60 57/60 56/60 56/60 56/60 46/50 46/50 46/50 46/50 44/50 43/50 43/50 95% 95% 93% 93% 93% 92% 92% 92% 92% 88% 86% 86% 9 1 60/60 60/60 59/60 58/60 58/60 48/50 48/50 48/50 48/50 48/50 48/50 48/50 100% 100% 98% 97% 97% 96% 96% 96% 96% 96% 96% 96% 3459 Covance 6329-212 Table 4 Survival Data Covance 6329-228 3M T-6316.1 PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 60 61 62 63 64 65 66 67 68 69 70 71 ---------------------------------------------------------------------------------------------------------------------- MALE 8 0 48/50 48/50 48/50 48/50 47/50 46/50 46/50 45/50 44/50 43/50 42/50 41/50 96% 96% 96% 96% 94% 92% 92% 90% 88% 86% 84% 82% 9 1 47/50 47/50 47/50 45/50 45/50 44/50 44/50 43/50 42/50 42/50 42/50 42/50 94% 94% 94% 90% 90% 88% 88% 86% 84% 84% 84% 84% FEMALE 8 0 43/50 43/50 43/50 41/50 40/50 40/50 40/50 40/50 40/50 39/50 38/50 38/50 86% 86% 86% 82% 80% 80% 80% 80% 80% 78% 76% 76% 9 1 48/50 47/50 45/50 43/50 43/50 43/50 43/50 43/50 41/50 41/50 40/50 40/50 96% 94% 90% 86% 86% 86% 86% 86% 82% 82% 80% 80% 3460 Covance 6329-212 Table 4 Survival Data Covance 6329-228 3M T-6316.1 PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 72 73 74 75 76 77 78 79 80 81 82 83 ---------------------------------------------------------------------------------------------------------------------- MALE 8 0 40/50 40/50 40/50 40/50 38/50 36/50 36/50 35/50 35/50 35/50 34/50 33/50 80% 80% 80% 80% 76% 72% 72% 70% 70% 70% 68% 66% 9 1 42/50 39/50 39/50 38/50 37/50 37/50 37/50 37/50 36/50 36/50 36/50 36/50 84% 78% 78% 76% 74% 74% 74% 74% 72% 72% 72% 72% FEMALE 8 0 37/50 36/50 35/50 35/50 35/50 35/50 34/50 33/50 33/50 32/50 30/50 29/50 74% 72% 70% 70% 70% 70% 68% 66% 66% 64% 60% 58% 9 1 40/50 39/50 39/50 39/50 39/50 38/50 38/50 38/50 37/50 35/50 35/50 34/50 80% 78% 78% 78% 78% 76% 76% 76% 74% 70% 70% 68% 3461 Covance 6329-212 Table 4 Survival Data Covance 6329-228 3M T-6316.1 PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 84 85 86 87 88 89 90 91 92 93 94 95 ---------------------------------------------------------------------------------------------------------------------- MALE 8 0 32/50 30/50 28/50 26/50 26/50 26/50 25/50 23/50 23/50 21/50 21/50 20/50 64% 60% 56% 52% 52% 52% 50% 46% 46% 42% 42% 40% 9 1 36/50 35/50 35/50 34/50 34/50 33/50 32/50 32/50 32/50 31/50 31/50 31/50 72% 70% 70% 68% 68% 66% 64% 64% 64% 62% 62% 62% FEMALE 8 0 29/50 28/50 28/50 27/50 26/50 25/50 25/50 25/50 24/50 22/50 19/50 19/50 58% 56% 56% 54% 52% 50% 50% 50% 48% 44% 38% 38% 9 1 34/50 33/50 33/50 33/50 33/50 31/50 31/50 30/50 28/50 28/50 27/50 27/50 68% 66% 66% 66% 66% 62% 62% 60% 56% 56% 54% 54% 3462 Covance 6329-212 Table 4 Survival Data Covance 6329-228 3M T-6316.1 PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 96 97 98 99 100 101 102 103 104 ---------------------------------------------------------------------------------------------------------------------- MALE 8 0 20/50 19/50 19/50 18/50 17/50 15/50 14/50 13/50 11/50 40% 38% 38% 36% 34% 30% 28% 26% 22% 9 1 29/50 26/50 26/50 26/50 25/50 23/50 23/50 23/50 20/50 58% 52% 52% 52% 50% 46% 46% 46% 40% FEMALE 8 0 17/50 17/50 17/50 17/50 16/50 15/50 14/50 14/50 13/50 34% 34% 34% 34% 32% 30% 28% 28% 26% 9 1 25/50 24/50 23/50 21/50 18/50 18/50 17/50 16/50 15/50 50% 48% 46% 42% 36% 36% 34% 32% 30% 3463 Covance 6329-212 Table 5 Covance 6329-228 3M T-6316.1 Summary of Clinical Observations PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-730 SEX: --MALE-- -FEMALE- GROUP: 8 9 8 9 CATEGORY DOSE: 0 1 0 1 KEYWORD QUALIFIER NUMBER: 70 70 70 70 ------------------------------------------------------------------------------------------------------------------------------------ *** TOP OF LIST *** APPEARANCE CONVULSIONS 0100 HEAD TILT LEFT 1064 RIGHT 1116 HUNCHED 4753 LIMITED USE LIMB-HIND-LEFT 0101 LIMBS-HIND 1000 MALOCCLUSION 13 7 19 9 MISSING TEETH 1100 OBESE 2101 PARALYSIS LIMB-FRONT-LEFT 0100 PROTRUDING PENIS 2100 SWOLLEN HEAD-MAXILLARY-RIGHT 0010 MOUTH 0010 NOSE 2000 PAW-HIND-LEFT 3001 PAW-HIND-RIGHT 1102 PAWS-HIND 1001 THIN 22 17 17 13 EXCESSIVE BLEEDING FROM PAWS, CAUTERIZED WITH SILVER NITRATE 000 3464 Covance 6329-212 Table 5 Covance 6329-228 3M T-6316.1 Summary of Clinical Observations PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-730 SEX: --MALE-- -FEMALE- GROUP: 8 9 8 9 CATEGORY DOSE: 0 1 0 1 KEYWORD QUALIFIER NUMBER: 70 70 70 70 ------------------------------------------------------------------------------------------------------------------------------------ BEHAVIOR AGGRESSIVE <UNSPECIFIED> MODERATE ATAXIC <UNSPECIFIED> MODERATE CIRCLING LEFT RIGHT HYPOACTIVE <UNSPECIFIED> MILD RECUMBENT LATERAL STERNAL SENSITIVE TO TOUCH ANIMAL IS CHEWING ON DISTAL PORTION OF TAIL COLD TO TOUCH ENTIRE BODY 0100 0100 2268 0010 0001 0010 16 10 10 13 0001 1001 0200 0312 1000 3114 DISCHARGE GENITAL RED IN COLOR CLEAR ORAL <UNSPECIFIED> 1301 0100 3465 Covance 6329-212 Table 5 Covance 6329-228 3M T-6316.1 Summary of Clinical Observations PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-730 SEX: --MALE-- -FEMALE- GROUP: 8 9 8 9 CATEGORY DOSE: 0 1 0 1 KEYWORD QUALIFIER NUMBER: 70 70 70 70 ------------------------------------------------------------------------------------------------------------------------------------ DISCHARGE CLEAR ORAL MODERATE NASAL RED IN COLOR RED DISCHARGE RED ORAL MUCOID DISCHARGE FROM RECTUM 0100 6196 1000 0010 0010 EXCRETION FEW FECES NO FECES NONFORMED FECES 22 11 20 18 10 7 8 7 15 14 1 6 EYES CLEAR DISCHARGE EYE-LEFT DISPLACED PUPIL EYE-RIGHT DILATED PUPIL EYES DISCOLORED/LIGHT RED EYES OPAQUE EYES PALE EYE-RIGHT EYES 0010 0010 1000 0010 0100 0100 4112 3466 Covance 6329-212 Table 5 Covance 6329-228 3M T-6316.1 Summary of Clinical Observations PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-730 SEX: --MALE-- -FEMALE- GROUP: 8 9 8 9 CATEGORY DOSE: 0 1 0 1 KEYWORD QUALIFIER NUMBER: 70 70 70 70 ------------------------------------------------------------------------------------------------------------------------------------ EYES RED DISCHARGE EYE-LEFT EYE-RIGHT EYES SCAB EYE-LEFT SQUINTED PERI-ORBITAL PERI-ORBITAL-LEFT PERI-ORBITAL-RIGHT 3366 1121 6 2 10 11 0100 7434 2001 0010 RESPIRATION AUDIBLE IRREGULAR LABORED 3120 5320 5232 SKIN & PELAGE ALOPECIA BROWN HAIR COAT PERINEAL AREA VENTRAL-ABDOMINAL VENTRAL-CERVICAL GENERALIZED BLUE SKIN VENTRAL-ABDOMINAL-LEFT 0110 3206 4321 1000 0010 0111 3467 Covance 6329-212 Table 5 Covance 6329-228 3M T-6316.1 Summary of Clinical Observations PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-730 SEX: --MALE-- -FEMALE- GROUP: 8 9 8 9 CATEGORY DOSE: 0 1 0 1 KEYWORD QUALIFIER NUMBER: 70 70 70 70 ------------------------------------------------------------------------------------------------------------------------------------ SKIN & PELAGE BLACK SKIN VENTRAL-ABDOMINAL VENTRAL-THORACIC-LEFT PALE ENTIRE BODY RED HAIR COAT DORSAL DORSAL-CERVICAL DORSAL-CERVICAL-RIGHT HEAD-ENTIRE HEAD-CRANIAL PERINEAL AREA SHOULDER-LEFT VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-CERVICAL ROUGH HAIR COAT RED SKIN TAIL SORE/SCAB AXILLARY REGION-LEFT AXILLARY REGION-RIGHT DORSAL DORSAL-CERVICAL DORSAL-CERVICAL-RIGHT 0120 0010 4433 0101 0100 0100 6125 0022 2010 0001 2031 0001 0010 4320 1000 0020 0031 2302 1200 1000 3468 Covance 6329-212 Table 5 Covance 6329-228 3M T-6316.1 Summary of Clinical Observations PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-730 SEX: --MALE-- -FEMALE- GROUP: 8 9 8 9 CATEGORY DOSE: 0 1 0 1 KEYWORD QUALIFIER NUMBER: 70 70 70 70 ------------------------------------------------------------------------------------------------------------------------------------ SKIN & PELAGE SORE/SCAB DORSAL-THORACIC-LEFT DORSAL-THORACIC-RIGHT HEAD-ENTIRE HEAD-CRANIAL HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT LATERAL-ABDOMINAL-RIGHT LATERAL-CERVICAL-LEFT LIMB-HIND-LEFT LUMBAR-RIGHT MOUTH PAW-FRONT-LEFT PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND PERINEAL AREA SCROTUM SCROTUM-RIGHT SHOULDER-RIGHT TAIL TAIL-DISTAL TAIL-PROXIMAL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT 1100 1000 0210 0001 0010 0101 1001 0001 0100 1000 0120 1000 7401 6313 1211 0001 1000 0100 0010 0001 1000 1001 4411 2101 3469 Covance 6329-212 Table 5 Covance 6329-228 3M T-6316.1 Summary of Clinical Observations PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-730 SEX: --MALE-- -FEMALE- GROUP: 8 9 8 9 CATEGORY DOSE: 0 1 0 1 KEYWORD QUALIFIER NUMBER: 70 70 70 70 ------------------------------------------------------------------------------------------------------------------------------------ SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL VENTRAL-CERVICAL-LEFT VENTRAL-CERVICAL-RIGHT VENTRAL-THORACIC VENTRAL-THORACIC-RIGHT WARM TO TOUCH ENTIRE BODY YELLOW HAIR COAT *** END OF LIST *** 1121 0112 0011 0100 0100 1100 0010 5375 3470 Covance 6329-212 Table 6 Covance 6329-228 3M T-6316.1 Summary of Mass Observations PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-730 SEX: --MALE-- -FEMALE- GROUP: 8 9 8 9 CATEGORY DOSE: 0 1 0 1 KEYWORD QUALIFIER NUMBER: 70 70 70 70 ------------------------------------------------------------------------------------------------------------------------------------ *** TOP OF LIST *** MASS(ES) AXILLARY REGION-LEFT WEEK 42 0001 WEEK 45 0010 WEEK 48 0001 WEEK 51 0002 WEEK 59 0010 WEEK 61 0010 WEEK 74 0001 WEEK 76 0020 WEEK 77 0010 WEEK 79 0001 WEEK 80 0010 WEEK 84 0001 WEEK 92 0010 WEEK 96 0021 WEEK 101 0001 WEEK 102 0010 AXILLARY REGION-RIGHT WEEK 28 0001 WEEK 39 0001 WEEK 40 0010 WEEK 42 0011 WEEK 55 0001 WEEK 57 0010 WEEK 59 0011 3471 Covance 6329-212 Table 6 Covance 6329-228 3M T-6316.1 Summary of Mass Observations PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-730 SEX: --MALE-- -FEMALE- GROUP: 8 9 8 9 CATEGORY DOSE: 0 1 0 1 KEYWORD QUALIFIER NUMBER: 70 70 70 70 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) AXILLARY REGION-RIGHT WEEK 60 WEEK 70 WEEK 73 WEEK 74 WEEK 75 WEEK 79 WEEK 80 WEEK 83 WEEK 84 WEEK 88 WEEK 91 WEEK 92 WEEK 96 WEEK 101 DORSAL WEEK 72 WEEK 83 WEEK 90 WEEK 95 HEAD-MAXILLARY-LEFT WEEK 93 WEEK 100 HEAD-MAXILLARY-RIGHT WEEK 66 0020 0001 0001 0001 0010 0010 0010 0010 0010 0001 0010 0010 0002 0011 0100 0001 0100 1000 0100 0100 0001 3472 Covance 6329-212 Table 6 Covance 6329-228 3M T-6316.1 Summary of Mass Observations PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-730 SEX: --MALE-- -FEMALE- GROUP: 8 9 8 9 CATEGORY DOSE: 0 1 0 1 KEYWORD QUALIFIER NUMBER: 70 70 70 70 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 74 INGUINAL REGION-LEFT WEEK 48 WEEK 64 WEEK 78 WEEK 79 WEEK 80 WEEK 84 WEEK 92 WEEK 95 WEEK 101 INGUINAL REGION-RIGHT WEEK 35 WEEK 40 WEEK 42 WEEK 56 WEEK 76 WEEK 84 WEEK 92 LIMB-FRONT-RIGHT WEEK 35 PAW-HIND-RIGHT WEEK 42 0100 0001 1000 0100 0010 0002 0001 0011 0001 0010 0001 0001 0001 0010 0010 0012 0002 0001 0001 3473 Covance 6329-212 Table 6 Covance 6329-228 3M T-6316.1 Summary of Mass Observations PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-730 SEX: --MALE-- -FEMALE- GROUP: 8 9 8 9 CATEGORY DOSE: 0 1 0 1 KEYWORD QUALIFIER NUMBER: 70 70 70 70 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) SHOULDER-LEFT WEEK 38 SHOULDER-RIGHT WEEK 19 WEEK 55 TAIL-PROXIMAL WEEK 75 VENTRAL-ABDOMINAL WEEK 32 WEEK 35 WEEK 40 WEEK 44 WEEK 45 WEEK 50 WEEK 56 WEEK 61 WEEK 62 WEEK 74 WEEK 75 WEEK 76 WEEK 78 WEEK 79 WEEK 80 WEEK 84 WEEK 100 0010 0010 0001 0001 1000 0001 0100 0001 0100 0011 0001 0001 0001 1010 1001 0100 1000 0100 0020 0100 0020 3474 Covance 6329-212 Table 6 Covance 6329-228 3M T-6316.1 Summary of Mass Observations PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-730 SEX: --MALE-- -FEMALE- GROUP: 8 9 8 9 CATEGORY DOSE: 0 1 0 1 KEYWORD QUALIFIER NUMBER: 70 70 70 70 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) VENTRAL-ABDOMINAL WEEK 102 WEEK 105 VENTRAL-ABDOMINAL-LEFT WEEK 28 WEEK 34 WEEK 40 WEEK 44 WEEK 46 WEEK 49 WEEK 50 WEEK 51 WEEK 61 WEEK 66 WEEK 69 WEEK 72 WEEK 73 WEEK 75 WEEK 76 WEEK 100 WEEK 101 WEEK 102 WEEK 103 VENTRAL-ABDOMINAL-RIGHT WEEK 28 0001 0100 1000 0001 1000 0021 0100 0020 0021 0020 0011 0002 0010 0100 0030 0001 0111 0010 0001 0010 0001 1000 3475 Covance 6329-212 Table 6 Covance 6329-228 3M T-6316.1 Summary of Mass Observations PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-730 SEX: --MALE-- -FEMALE- GROUP: 8 9 8 9 CATEGORY DOSE: 0 1 0 1 KEYWORD QUALIFIER NUMBER: 70 70 70 70 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 33 WEEK 40 WEEK 44 WEEK 45 WEEK 48 WEEK 51 WEEK 53 WEEK 61 WEEK 63 WEEK 64 WEEK 66 WEEK 70 WEEK 74 WEEK 76 WEEK 78 WEEK 79 WEEK 80 WEEK 84 WEEK 92 WEEK 101 VENTRAL-CERVICAL WEEK 36 WEEK 46 WEEK 54 1000 0001 0001 0001 0100 0010 0020 0001 1000 0001 0010 0001 0001 0100 1000 1001 0001 0210 0001 0001 0010 0020 0001 3476 Covance 6329-212 Table 6 Covance 6329-228 3M T-6316.1 Summary of Mass Observations PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-730 SEX: --MALE-- -FEMALE- GROUP: 8 9 8 9 CATEGORY DOSE: 0 1 0 1 KEYWORD QUALIFIER NUMBER: 70 70 70 70 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) VENTRAL-CERVICAL WEEK 65 WEEK 75 WEEK 77 WEEK 80 WEEK 84 WEEK 88 VENTRAL-CERVICAL-LEFT WEEK 31 WEEK 38 WEEK 70 WEEK 95 VENTRAL-THORACIC WEEK 70 WEEK 80 VENTRAL-THORACIC-LEFT WEEK 65 WEEK 74 VENTRAL-THORACIC-RIGHT WEEK 82 0010 0001 0001 0001 0001 0001 0010 0001 0001 0010 0010 0010 0001 0010 0100 WART LIKE LESION(S) DORSAL WEEK 41 WEEK 59 1000 0100 3477 Covance 6329-212 Table 6 Covance 6329-228 3M T-6316.1 Summary of Mass Observations PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-730 SEX: --MALE-- -FEMALE- GROUP: 8 9 8 9 CATEGORY DOSE: 0 1 0 1 KEYWORD QUALIFIER NUMBER: 70 70 70 70 ------------------------------------------------------------------------------------------------------------------------------------ WART LIKE LESION(S) DORSAL WEEK 62 WEEK 72 WEEK 76 WEEK 82 WEEK 93 EAR-LEFT WEEK 92 HEAD-MAXILLARY-RIGHT WEEK 64 WEEK 79 LIMB-FRONT-RIGHT WEEK 105 MOUTH WEEK 54 WEEK 100 NOSE WEEK 46 WEEK 68 WEEK 100 TAIL-MID WEEK 101 TAIL-PROXIMAL WEEK 64 WEEK 74 0100 0100 0100 0100 1000 0010 1000 0001 0100 0010 0100 0010 1000 0100 0100 0100 1000 3478 Covance 6329-212 Table 6 Covance 6329-228 3M T-6316.1 Summary of Mass Observations PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-730 SEX: --MALE-- -FEMALE- GROUP: 8 9 8 9 CATEGORY DOSE: 0 1 0 1 KEYWORD QUALIFIER NUMBER: 70 70 70 70 ------------------------------------------------------------------------------------------------------------------------------------ WART LIKE LESION(S) VENTRAL-ABDOMINAL WEEK 20 WEEK 58 WEEK 92 VENTRAL-ABDOMINAL-RIGHT WEEK 82 VENTRAL-THORACIC-RIGHT WEEK 82 WEEK 101 *** END OF LIST *** 1000 0100 0100 0100 0100 0100 3479 Covance 6329-212 Table 7 Covance 6329-228 3M T-6316.1 Summary of Body Weight Data (g) PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------MALE--------- --------FEMALE-------- GROUP: 8 9 8 9 DOSE: 0 1 0 1 WEEK UNITS: PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 1 N 70 MEAN 204 S.D. 18.8 70 202 19.4 70 152 10.8 70 152 11.7 2 N 70 MEAN 260 S.D. 21.9 70 260 22.1 70 179 13.9 70 179 13.3 3 N 70 MEAN 309 S.D. 25.3 70 309 25.4 70 202 16.1 70 203 15.3 4 N 70 MEAN 351 S.D. 28.4 70 350 28.4 70 224 19.6 70 223 17.6 5 N 65 MEAN 381 S.D. 33.1 65 383 33.4 65 240 19.9 65 236 17.8 6 N 65 MEAN 409 S.D. 36.9 65 413 36.4 65 253 22.3 65 251 20.1 7 N 65 MEAN 433 S.D. 40.2 65 438 40.4 65 263 24.5 65 260 21.4 3480 Covance 6329-212 Table 7 Covance 6329-228 3M T-6316.1 Summary of Body Weight Data (g) PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------MALE--------- --------FEMALE-------- GROUP: 8 9 8 9 DOSE: 0 1 0 1 WEEK UNITS: PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 8 N 65 MEAN 451 S.D. 43.0 65 454 42.9 64 272 25.2 64 267 23.3 9 N 65 MEAN 468 S.D. 45.9 65 472 45.3 64 282 27.6 65 274 23.9 10 N 65 MEAN 484 S.D. 49.7 65 490 47.4 64 287 28.4 65 281 25.7 11 N 65 MEAN 496 S.D. 54.3 65 503 48.9 64 287 29.0 65 284 26.3 12 N 64 MEAN 510 S.D. 59.2 65 517 51.2 64 296 30.2 65 288 27.1 13 N 64 MEAN 523 S.D. 58.0 65 529 53.4 64 301 30.7 65 294 27.3 14 N 64 MEAN 531 S.D. 58.7 65 537 54.4 64 306 31.4 65 298 29.8 3481 Covance 6329-212 Table 7 Covance 6329-228 3M T-6316.1 Summary of Body Weight Data (g) PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------MALE--------- --------FEMALE-------- GROUP: 8 9 8 9 DOSE: 0 1 0 1 WEEK UNITS: PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 15 N 59 MEAN 541 S.D. 60.2 60 545 56.4 59 307 34.1 60 302 30.8 16 N 59 MEAN 553 S.D. 60.8 60 555 59.9 59 314 35.0 60 307 32.3 17 N 59 MEAN 562 S.D. 61.8 60 564 62.7 59 319 35.8 60 309 33.9 21 N 59 MEAN 591 S.D. 68.4 60 593 70.0 59 332 41.3 60 325 39.3 25 N 59 MEAN 617 S.D. 73.3 60 616 74.5 59 345 46.7 60 340 46.4 29 N 59 MEAN 631 S.D. 76.8 60 630 77.5 59 354 48.3 60 348 51.5 33 N 59 MEAN 652 S.D. 81.0 60 655 84.4 58 371 53.9 60 366 60.0 3482 Covance 6329-212 Table 7 Covance 6329-228 3M T-6316.1 Summary of Body Weight Data (g) PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------MALE--------- --------FEMALE-------- GROUP: 8 9 8 9 DOSE: 0 1 0 1 WEEK UNITS: PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 37 N 59 MEAN 681 S.D. 85.7 60 674 89.2 58 389 60.1 60 380 60.3 41 N 59 MEAN 697 S.D. 90.0 60 693 93.2 58 402 63.8 60 392 64.5 45 N 59 MEAN 719 S.D. 94.9 58 709 99.8 58 416 68.8 60 407 68.9 49 N 59 MEAN 732 S.D. 98.6 58 726 105.2 57 431 74.4 60 423 72.3 53 N 58 MEAN 751 S.D. 102.7 58 738 110.7 56 445 78.1 58 436 74.2 57 N 48 MEAN 759 S.D. 107.8 48 741 123.5 45 452 87.7 48 456 87.5 61 N 48 MEAN 771 S.D. 116.1 47 748 138.9 43 474 94.0 48 474 95.6 3483 Covance 6329-212 Table 7 Covance 6329-228 3M T-6316.1 Summary of Body Weight Data (g) PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------MALE--------- --------FEMALE-------- GROUP: 8 9 8 9 DOSE: 0 1 0 1 WEEK UNITS: PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 65 N 47 MEAN 776 S.D. 116.3 45 761 143.0 40 492 91.2 43 481 102.5 69 N 44 MEAN 768 S.D. 135.9 42 779 131.8 40 498 97.9 41 485 102.5 73 N 40 MEAN 791 S.D. 140.6 42 773 142.6 37 511 98.6 40 499 103.1 77 N 38 MEAN 791 S.D. 154.4 37 773 126.5 35 522 96.8 39 498 106.3 81 N 35 MEAN 797 S.D. 146.7 36 788 127.3 33 516 102.6 37 497 111.1 85 N 32 MEAN 786 S.D. 162.7 36 787 132.7 29 531 97.3 34 510 120.1 89 N 26 MEAN 795 S.D. 158.6 34 772 144.2 26 532 97.6 33 511 128.1 3484 Covance 6329-212 Table 7 Covance 6329-228 3M T-6316.1 Summary of Body Weight Data (g) PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------MALE--------- --------FEMALE-------- GROUP: 8 9 8 9 DOSE: 0 1 0 1 WEEK UNITS: PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 93 N 23 MEAN 791 S.D. 157.9 32 771 140.7 24 518 110.6 28 519 133.6 97 N 20 MEAN 786 S.D. 119.5 28 758 153.9 17 542 102.5 25 504 111.2 101 N MEAN S.D. 17 710 148.5 25 727 144.4 16 517 108.3 18 517 77.6 105 N MEAN S.D. 11 656 206.2 20 739 127.5 13 515 126.2 15 515 69.6 3485 Covance 6329-212 Table 8 Covance 6329-228 3M T-6316.1 Summary of Body Weight Change Data (g) PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------MALE--------- --------FEMALE-------- GROUP: 8 9 8 9 DOSE: 0 1 0 1 WEEK UNITS: PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 1-15 N MEAN S.D. 59 337 50.0 60 342 47.4 59 155 28.3 60 149 25.5 15-29 N MEAN S.D. 59 90 23.0 60 85 28.0 59 47 21.9 60 46 26.9 29-57 N MEAN S.D. 48 129 49.3 48 116 53.5 45 96 54.4 48 107 47.6 57-105 N MEAN S.D. 11 -70 194.7 20 38 78.3 13 94 89.1 15 99 37.2 1-105 N MEAN S.D. 11 455 200.2 20 547 124.1 13 366 120.9 15 364 67.3 3486 Covance 6329-212 Table 9 Covance 6329-228 3M T-6316.1 Summary of Food Consumption Data (g) PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------MALE--------- --------FEMALE-------- GROUP: 8 9 8 9 DOSE: 0 1 0 1 WEEK UNITS: PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 1 N 70 MEAN 167 S.D. 12.9 69 166 13.9 46 128 13.5 57 126 12.6 2 N 69 MEAN 174 S.D. 15.6 69 175 17.4 51 129 13.9 55 129 13.5 3 N 69 MEAN 180 S.D. 15.6 67 180 21.6 63 137 19.3 66 138 20.1 4 N 63 MEAN 182 S.D. 16.2 64 183 17.7 50 138 13.6 58 138 18.6 5 N 63 MEAN 185 S.D. 17.7 60 186 19.2 55 136 14.5 53 137 12.3 6 N 62 MEAN 186 S.D. 19.2 62 187 19.5 55 141 18.8 52 139 13.4 7 N 65 MEAN 189 S.D. 19.1 64 189 20.1 61 141 17.8 57 137 15.2 3487 Covance 6329-212 Table 9 Covance 6329-228 3M T-6316.1 Summary of Food Consumption Data (g) PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------MALE--------- --------FEMALE-------- GROUP: 8 9 8 9 DOSE: 0 1 0 1 WEEK UNITS: PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 8 N 63 MEAN 188 S.D. 19.6 61 188 20.8 54 139 17.4 48 137 17.0 9 N 63 MEAN 188 S.D. 18.9 64 191 21.0 57 137 18.4 58 135 16.0 10 N 63 MEAN 186 S.D. 28.8 63 190 17.6 51 138 15.1 51 136 20.1 11 N 62 MEAN 187 S.D. 19.9 61 187 16.9 52 132 14.9 51 127 13.7 12 N 63 MEAN 192 S.D. 19.4 64 194 17.6 53 131 16.7 55 134 13.9 13 N 64 MEAN 194 S.D. 19.7 64 195 18.4 60 136 17.6 61 140 19.4 14 N 47 MEAN 196 S.D. 19.0 50 189 16.2 42 131 13.7 38 138 20.2 3488 Covance 6329-212 Table 9 Covance 6329-228 3M T-6316.1 Summary of Food Consumption Data (g) PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------MALE--------- --------FEMALE-------- GROUP: 8 9 8 9 DOSE: 0 1 0 1 WEEK UNITS: PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 15 N 59 MEAN 190 S.D. 19.5 58 193 18.4 56 132 14.8 54 138 22.9 16 N 55 MEAN 192 S.D. 20.3 60 193 20.6 47 131 18.2 48 134 14.7 20 N 58 MEAN 187 S.D. 19.9 57 187 19.6 56 136 19.3 52 141 24.4 24 N 58 MEAN 191 S.D. 21.3 60 190 17.9 57 144 28.2 55 141 20.5 28 N 55 MEAN 194 S.D. 20.0 56 194 18.0 53 150 21.4 57 151 23.3 32 N 59 MEAN 192 S.D. 21.3 60 197 20.7 57 149 21.9 59 151 23.2 36 N 58 MEAN 190 S.D. 18.5 56 192 19.4 57 144 19.1 54 148 15.9 3489 Covance 6329-212 Table 9 Covance 6329-228 3M T-6316.1 Summary of Food Consumption Data (g) PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------MALE--------- --------FEMALE-------- GROUP: 8 9 8 9 DOSE: 0 1 0 1 WEEK UNITS: PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 40 N 59 MEAN 189 S.D. 19.9 60 189 19.6 57 143 17.7 57 144 20.1 44 N 58 MEAN 185 S.D. 21.8 58 186 21.4 58 143 17.3 58 145 18.6 48 N 59 MEAN 182 S.D. 21.8 58 187 21.4 56 144 19.9 59 146 27.0 52 N 58 MEAN 189 S.D. 21.7 57 183 20.7 55 146 27.1 51 148 27.4 56 N 45 MEAN 182 S.D. 22.0 47 179 28.4 44 143 34.3 43 144 22.3 60 N 46 MEAN 188 S.D. 29.9 45 185 33.4 39 151 26.7 44 149 31.0 64 N 47 MEAN 188 S.D. 36.6 44 188 34.1 38 157 26.5 41 148 39.1 3490 Covance 6329-212 Table 9 Covance 6329-228 3M T-6316.1 Summary of Food Consumption Data (g) PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------MALE--------- --------FEMALE-------- GROUP: 8 9 8 9 DOSE: 0 1 0 1 WEEK UNITS: PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 68 N 43 MEAN 184 S.D. 42.8 41 195 26.5 39 161 37.5 41 158 26.3 72 N 38 MEAN 195 S.D. 34.3 41 189 40.9 36 165 40.8 39 158 25.4 76 N 37 MEAN 191 S.D. 43.2 37 193 24.7 28 160 27.4 36 156 29.5 80 N 34 MEAN 177 S.D. 36.4 33 181 19.5 28 139 23.4 34 132 40.6 84 N 32 MEAN 163 S.D. 51.1 34 176 21.4 26 133 39.1 32 138 36.6 88 N 26 MEAN 169 S.D. 40.0 32 173 27.0 25 141 27.0 33 137 36.7 92 N 22 MEAN 166 S.D. 43.5 31 167 31.6 21 129 40.7 28 127 34.7 3491 Covance 6329-212 Table 9 Covance 6329-228 3M T-6316.1 Summary of Food Consumption Data (g) PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------MALE--------- --------FEMALE-------- GROUP: 8 9 8 9 DOSE: 0 1 0 1 WEEK UNITS: PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 96 N 20 MEAN 158 S.D. 41.0 27 157 42.0 17 146 20.2 23 124 48.9 100 N MEAN S.D. 14 141 65.2 24 173 29.0 15 140 32.4 14 139 31.4 104 N MEAN S.D. 8 176 44.6 20 167 35.5 12 141 32.0 12 144 28.0 3492 Covance 6329-212 Table 10 Covance 6329-228 3M T-6316.1 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---MALE---- ---FEMALE-- GROUP: 9 9 DOSE: 1 1 WEEK UNITS: PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 1 N 69 57 MEAN 0.103 0.108 S.D. 0.007 0.010 2 N 69 55 MEAN 0.088 0.096 S.D. 0.007 0.009 3 N 67 66 MEAN 0.078 0.092 S.D. 0.008 0.013 4 N 64 58 MEAN 0.071 0.086 S.D. 0.004 0.011 5 N 60 53 MEAN 0.066 0.080 S.D. 0.004 0.005 6 N 62 52 MEAN 0.063 0.077 S.D. 0.003 0.007 7 N 64 56 MEAN 0.061 0.075 S.D. 0.005 0.008 3493 Covance 6329-212 Table 10 Covance 6329-228 3M T-6316.1 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---MALE---- ---FEMALE-- GROUP: 9 9 DOSE: 1 1 WEEK UNITS: PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 8 N 61 47 MEAN 0.058 0.072 S.D. 0.005 0.010 9 N 64 58 MEAN 0.057 0.070 S.D. 0.004 0.009 10 N MEAN S.D. 63 0.055 0.004 51 0.069 0.010 11 N MEAN S.D. 61 0.053 0.003 51 0.063 0.007 12 N MEAN S.D. 64 0.053 0.004 55 0.066 0.006 13 N MEAN S.D. 64 0.052 0.003 61 0.067 0.009 14 N MEAN S.D. 50 0.051 0.003 38 0.065 0.009 3494 Covance 6329-212 Table 10 Covance 6329-228 3M T-6316.1 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---MALE---- ---FEMALE-- GROUP: 9 9 DOSE: 1 1 WEEK UNITS: PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 15 N MEAN S.D. 58 0.050 0.003 54 0.064 0.012 16 N MEAN S.D. 60 0.049 0.003 48 0.062 0.006 20 N MEAN S.D. 57 0.045 0.003 52 0.062 0.012 24 N MEAN S.D. 60 0.045 0.004 55 0.060 0.009 28 N MEAN S.D. 56 0.044 0.004 57 0.062 0.008 32 N MEAN S.D. 60 0.043 0.003 59 0.060 0.010 36 N MEAN S.D. 56 0.041 0.003 54 0.056 0.007 3495 Covance 6329-212 Table 10 Covance 6329-228 3M T-6316.1 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---MALE---- ---FEMALE-- GROUP: 9 9 DOSE: 1 1 WEEK UNITS: PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 40 N MEAN S.D. 60 0.039 0.003 57 0.053 0.007 44 N MEAN S.D. 58 0.038 0.003 58 0.052 0.006 48 N MEAN S.D. 58 0.037 0.003 59 0.050 0.009 52 N MEAN S.D. 57 0.036 0.003 51 0.049 0.008 56 N MEAN S.D. 47 0.035 0.005 43 0.046 0.005 60 N MEAN S.D. 45 0.035 0.004 44 0.045 0.008 64 N MEAN S.D. 44 0.035 0.005 41 0.044 0.012 3496 Covance 6329-212 Table 10 Covance 6329-228 3M T-6316.1 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---MALE---- ---FEMALE-- GROUP: 9 9 DOSE: 1 1 WEEK UNITS: PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 68 N MEAN S.D. 41 0.036 0.005 41 0.047 0.007 72 N MEAN S.D. 41 0.035 0.006 39 0.046 0.007 76 N MEAN S.D. 37 0.036 0.005 36 0.046 0.008 80 N MEAN S.D. 33 0.033 0.004 34 0.039 0.011 84 N MEAN S.D. 34 0.032 0.004 32 0.039 0.009 88 N MEAN S.D. 32 0.032 0.004 33 0.039 0.009 92 N MEAN S.D. 31 0.031 0.007 28 0.036 0.010 3497 Covance 6329-212 Table 10 Covance 6329-228 3M T-6316.1 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---MALE---- ---FEMALE-- GROUP: 9 9 DOSE: 1 1 WEEK UNITS: PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 96 N MEAN S.D. 27 0.029 0.007 23 0.034 0.013 100 N MEAN S.D. 24 0.034 0.003 14 0.039 0.012 104 N MEAN S.D. 20 0.032 0.004 12 0.040 0.007 3498 Table 11 Summary of Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/.L G/DL % FL PG % X103/.L ------ -------- -------- -------- -------- -------- -------- -------- 0 MEAN 8.32 15.2 44.3 53.4 18.3 34.2 1094 S.D. .838 1.18 3.23 2.55 .80 .45 94.6 N 10 10 10 10 10 10 10 1 MEAN 8.38 15.0 44.1 52.6 17.9 34.0 1094 S.D. .411 .64 1.62 1.35 .53 .31 90.4 N 10 10 10 10 10 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3499 Covance 6329-212 Table 11 Summary of Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 DOSE ppm WBC X103/.L N-SEG X103/.L LYMPH X103/.L MONO X103/.L EOSIN X103/.L BASO X103/.L N-SEG% LYMPH% MONO% EOSIN% BASO% ------ -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 10.4 1.3 7.9 .9 .3 .0 13 76 9 3 0 S.D. 2.18 .28 2.04 .23 .12 .00 2.6 5.2 2.9 1.3 .0 N 10 10 10 10 10 10 10 10 10 10 10 1 MEAN 10.2 1.3 7.5 1.1 .3 .0 13 74 11 2 0 S.D. 2.12 .39 1.72 .50 .18 .03 4.1 4.4 3.9 1.3 .0 N 10 10 10 10 10 10 10 10 10 10 10 3500 Table 11 Summary of Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/.L G/DL % FL PG % X103/.L ------ -------- -------- -------- -------- -------- -------- -------- 0 MEAN 7.82 14.7 43.1 55.1 18.8 34.2 1064 S.D. .358 .73 2.10 1.05 .53 .45 88.2 N 10 10 10 10 10 10 10 1 MEAN 8.01 15.2 44.2 55.3 19.0 34.4 1112 S.D. .350 .39 1.44 1.32 .53 .52 83.6 N 10 10 10 10 10 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3501 Covance 6329-212 Table 11 Summary of Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 DOSE ppm WBC X103/.L N-SEG X103/.L LYMPH X103/.L MONO X103/.L EOSIN X103/.L BASO X103/.L N-SEG% LYMPH% MONO% EOSIN% BASO% ------ -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 7.3 .8 5.8 .5 .2 .0 12 78 7 3 0 S.D. 2.61 .38 2.24 .16 .09 .00 4.3 4.7 1.7 .9 .0 N 10 10 10 10 10 10 10 10 10 10 10 1 MEAN 6.5 .9 5.0 .4 .2 .0 15 75 7 3 0 S.D. 1.23 .20 1.37 .17 .06 .00 5.0 8.7 3.7 1.0 .0 N 10 10 10 10 10 10 10 10 10 10 10 3502 Table 12 Summary of Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/.L G/DL % FL PG % X103/.L ------ -------- -------- -------- -------- -------- -------- -------- 0 MEAN 8.22 15.2 44.6 54.3 18.5 34.0 1083 S.D. .430 .67 1.98 1.63 .62 .36 109.2 N 10 10 10 10 10 10 10 1 MEAN 8.28 14.8 43.9 53.1 17.9 * 33.8 1112 S.D. .487 .78 2.19 1.28 .54 .39 83.2 N 10 10 10 10 10 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3503 Covance 6329-212 Table 12 Summary of Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 DOSE ppm WBC X103/.L N-SEG X103/.L LYMPH X103/.L MONO X103/.L EOSIN BASO X103/.L X103/.L N-SEG% LYMPH% MONO% EOSIN% BASO% ------ -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 8.6 1.7 5.7 1.0 .2 .0 19 66 11 3 0 S.D. 1.85 .81 1.12 .34 .13 .04 5.4 8.0 4.0 1.6 .5 N 10 10 10 10 10 10 10 10 10 10 10 1 MEAN 8.9 1.5 5.9 1.2 .2 .1 17 66 14 2 1 S.D. 1.34 .28 1.17 .67 .08 .13 4.0 7.6 6.2 1.0 .9 N 10 10 10 10 10 10 10 10 10 10 10 3504 Table 12 Summary of Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/.L G/DL % FL PG % X103/.L ------ -------- -------- -------- -------- -------- -------- -------- 0 MEAN 7.31 13.9 40.6 55.6 19.1 34.3 1038 S.D. .267 .75 1.79 1.46 .66 .50 101.1 N 10 10 10 10 10 10 10 1 MEAN 7.63 14.7 * 42.5 * 55.8 19.3 34.6 1098 S.D. .451 .54 1.93 1.45 .58 .41 161.0 N 10 10 10 10 10 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3505 Covance 6329-212 Table 12 Summary of Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 DOSE ppm WBC X103/.L N-SEG X103/.L LYMPH X103/.L MONO X103/.L EOSIN BASO X103/.L X103/.L N-SEG% LYMPH% MONO% EOSIN% BASO% ------ -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 6.4 1.0 4.6 .6 .2 .0 15 73 9 2 0 S.D. 2.32 .72 1.46 .28 .15 .00 5.7 7.7 2.3 1.2 .5 N 10 10 10 10 10 10 10 10 10 10 10 1 MEAN 5.8 .9 4.2 .5 .1 .0 16 72 9 2 0 S.D. 1.90 .30 1.63 .21 .04 .00 5.8 9.9 3.6 .8 .3 N 10 10 10 10 10 10 10 10 10 10 10 3506 Table 13 Summary of Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/.L G/DL % FL PG % X103/.L ------ -------- -------- -------- -------- -------- -------- -------- 0 MEAN 8.20 15.2 42.6 52.0 18.5 35.6 1171 S.D. .530 .78 2.22 1.89 .69 .24 158.9 N 10 10 10 10 10 10 10 1 MEAN 8.42 15.2 43.4 51.6 18.1 35.2 * 1131 S.D. .439 .71 1.97 2.54 1.04 .38 185.5 N 10 10 10 10 10 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3507 Covance 6329-212 Table 13 Summary of Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 DOSE ppm WBC X103/.L N-SEG X103/.L LYMPH X103/.L MONO X103/.L EOSIN BASO X103/.L X103/.L N-SEG% LYMPH% MONO% EOSIN% BASO% ------ -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 6.8 2.0 3.9 .8 .1 .0 29 57 11 2 0 S.D. 1.64 .73 1.29 .34 .05 .03 8.6 10.4 4.7 .9 .4 N 10 10 10 10 10 10 10 10 10 10 10 1 MEAN 6.7 2.0 3.8 .8 .1 .0 28 57 12 2 0 S.D. 2.12 1.17 .93 .29 .05 .00 7.3 6.7 2.7 .6 .0 N 10 10 10 10 10 10 10 10 10 10 10 3508 Table 13 Summary of Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/.L G/DL % FL PG % X103/.L ------ -------- -------- -------- -------- -------- -------- -------- 0 MEAN 7.71 14.9 41.4 53.8 19.4 36.0 S.D. .362 .58 1.77 1.63 .67 .46 N 10 10 10 10 10 10 941 117.1 10 1 MEAN 7.78 14.9 41.6 53.5 19.2 35.7 1053 * S.D. .435 .46 1.16 1.75 .61 .41 102.5 N 10 10 10 10 10 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3509 Covance 6329-212 Table 13 Summary of Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 DOSE ppm WBC X103/.L N-SEG X103/.L LYMPH X103/.L MONO X103/.L EOSIN BASO X103/.L X103/.L N-SEG% LYMPH% MONO% EOSIN% BASO% ------ -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 5.2 1.4 3.2 .6 .1 .0 27 60 11 2 0 S.D. 1.64 .48 1.16 .13 .03 .00 4.7 5.8 2.3 .8 .0 N 10 10 10 10 10 10 10 10 10 10 10 1 MEAN 5.1 1.3 3.2 .6 .1 .0 26 60 12 2 0 S.D. 2.18 .73 1.66 .24 .04 .03 8.6 9.5 4.6 .7 .4 N 10 10 10 10 10 10 10 10 10 10 10 3510 Table 14 Summary of Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G ------ -------- 0 MEAN 7 S.D. 2.6 N 5 1 MEAN 7 S.D. 3.8 N 5 Covance 6329-212 Covance 6329-228 3M T-6316.1 3511 Table 14 Summary of Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G ------ -------- 0 MEAN 3 S.D. 1.1 N 5 1 MEAN 4 S.D. 2.3 N 5 Covance 6329-212 Covance 6329-228 3M T-6316.1 3512 Table 15 Summary of Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 113 14 .7 6.9 4.8 2.1 .0 59 S.D. 9.6 2.1 .10 .40 .22 .28 .05 7.0 N 10 10 10 10 10 10 10 10 1 MEAN 114 13 .6 7.0 4.7 2.3 .0 63 S.D. 7.4 2.1 .11 .32 .19 .26 .05 12.8 N 10 10 10 10 10 10 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3513 Table 15 Summary of Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 110 21.4 10 39 8.1 10 2 2.1 10 10.6 .19 10 7.3 .63 10 147 .8 10 5.9 .39 10 106 1.9 10 1 MEAN S.D. N 111 14.9 10 48 21.2 10 3 2.2 10 10.6 .24 10 7.0 .31 10 147 .8 10 6.3 * .31 10 107 .6 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3514 Table 15 Summary of Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 114 14 .8 8.2 6.1 2.1 .2 94 S.D. 8.7 2.3 .14 .60 .52 .22 .16 22.1 N 10 10 10 10 10 10 10 10 1 MEAN 110 15 .7 7.9 5.8 2.1 .1 88 S.D. 3.8 2.4 .12 .68 .66 .27 .13 20.3 N 10 10 10 10 10 10 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3515 Table 15 Summary of Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 110 58.6 10 44 24.9 10 2 1.3 10 11.4 .54 10 6.0 .44 10 145 1.6 10 5.8 .38 10 106 1.8 10 1 MEAN S.D. N 108 24.1 10 44 19.1 10 2 2.0 10 11.2 .29 10 6.4 * .43 10 147 * .8 10 6.0 .36 10 107 2.2 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3516 Table 15 Summary of Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G ------ -------- 0 MEAN 6 S.D. 1.3 N 5 1 MEAN 5 S.D. 1.1 N 5 Covance 6329-212 Covance 6329-228 3M T-6316.1 3517 Table 15 Summary of Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G ------ -------- 0 MEAN 3 S.D. .9 N 5 1 MEAN 5 S.D. 1.6 N 5 Covance 6329-212 Covance 6329-228 3M T-6316.1 3518 Table 16 Summary of Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 103 12 .6 6.9 4.6 2.2 .1 75 S.D. 10.1 1.2 .06 .32 .17 .28 .05 12.5 N 10 10 10 10 10 10 10 10 1 MEAN 111 * 13 .6 7.1 4.6 2.5 .2 84 S.D. 7.3 .9 .04 .29 .18 .25 .05 17.1 N 10 10 10 10 10 10 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3519 Table 16 Summary of Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 120 21.8 10 43 23.3 10 2 .8 10 10.6 .16 10 7.0 .41 10 146 1.2 10 5.9 .34 10 105 2.0 10 1 MEAN S.D. N 110 20.4 10 45 18.1 10 1 .8 10 10.8 * .20 10 7.0 .47 10 147 1.1 10 6.3 * .29 10 105 2.6 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3520 Table 16 Summary of Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 106 14 .7 8.3 6.1 2.2 S.D. 8.0 1.7 .03 .41 .32 .23 N 10 10 10 10 10 10 .2 117 .00 27.5 10 10 1 MEAN S.D. N 104 7.2 10 13 2.2 10 .7 * .05 10 8.2 .45 10 5.9 .64 10 2.3 .50 10 .2 111 .06 33.8 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3521 Table 16 Summary of Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 115 50.2 10 58 38.8 10 2 1.9 10 11.3 .38 10 5.6 .59 10 144 1.3 10 5.2 .50 10 103 2.7 10 1 MEAN S.D. N 178 168.9 10 96 113.8 10 4 6.3 10 11.2 .43 10 6.1 .59 10 146 * 1.3 10 5.5 .54 10 104 2.3 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3522 Table 17 Summary of Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 119 12 .7 7.1 4.6 2.5 S.D. 19.5 2.0 .09 .35 .37 .29 N 10 10 10 10 10 10 .2 105 .05 33.8 10 10 1 MEAN 127 12 .7 7.1 4.6 2.5 S.D. 13.2 2.3 .03 .31 .31 .30 N 10 10 10 10 10 10 .2 102 .05 42.1 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3523 Table 17 Summary of Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 108 55.4 10 77 118.8 10 2 1.1 10 10.3 .55 10 6.2 .89 10 149 3.5 10 6.1 .58 10 106 3.0 10 1 MEAN S.D. N 110 40.0 10 42 12.3 10 1 1.3 10 10.5 .34 10 6.4 .53 10 150 4.3 10 6.4 .50 10 107 3.8 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3524 Table 17 Summary of Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 113 11 .8 8.4 5.9 2.4 S.D. 17.5 1.1 .07 .44 .36 .34 N 10 10 10 10 10 10 .3 142 .22 34.0 10 10 1 MEAN 108 14 * .8 8.5 5.8 2.7 S.D. 8.9 2.5 .07 .47 .40 .41 N 10 10 10 10 10 10 .2 126 .06 20.8 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3525 Table 17 Summary of Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 100 29.1 10 54 24.7 10 2 1.0 10 11.1 .44 10 5.4 1.05 10 147 4.1 10 5.4 .37 10 102 2.6 10 1 MEAN S.D. N 156 160.1 10 72 62.1 10 2 2.6 10 11.0 .50 10 5.7 1.14 10 147 2.3 10 5.3 .68 10 104 3.0 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3526 Table 18 Summary of Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH ------ -------- -------- -------- 0 MEAN 22.7 1.015 S.D. 15.31 .0116 N 10 10 7.1 .32 10 1 MEAN 21.9 1.017 S.D. 13.22 .0085 N 10 10 7.2 .24 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3527 Table 18 Summary of Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH ------ -------- -------- -------- 0 MEAN 12.5 1.017 S.D. 13.88 .0078 N 10 10 6.8 .26 10 1 MEAN 22.0 1.012 S.D. 18.74 .0112 N 10 10 6.7 .26 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3528 Table 19 Summary of Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH ------ -------- -------- -------- 0 MEAN 13.8 1.022 S.D. 12.00 .0081 N 10 10 6.9 .39 10 1 MEAN 15.4 1.026 S.D. 13.27 .0125 N 10 10 7.0 .28 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3529 Table 19 Summary of Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH ------ -------- -------- -------- 0 MEAN 17.1 1.019 S.D. 17.79 .0134 N 10 10 6.6 .21 10 1 MEAN 20.0 1.018 S.D. 17.04 .0126 N 10 10 6.6 .16 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3530 Table 20 Summary of Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH ------ -------- -------- -------- 0 MEAN 14.7 1.026 S.D. 12.83 .0110 N 10 10 7.0 .33 10 1 MEAN 10.5 1.030 S.D. 6.47 .0123 N 10 10 6.9 .21 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3531 Table 20 Summary of Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH ------ -------- -------- -------- 0 MEAN 27.2 1.014 S.D. 19.88 .0106 N 10 10 6.5 .24 10 1 MEAN 13.0 1.019 S.D. 10.19 .0093 N 10 10 6.5 .33 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3532 Table 21 Summary of Urine Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL ------ -------- -------- -------- -------- 0 MEAN S.D. N 24 16.7 10 80 72.9 10 .35 .079 9 1.06 .227 9 1 MEAN S.D. N 30 14.3 10 89 43.9 10 .51 * .151 9 1.53 * .425 9 Covance 6329-212 Covance 6329-228 3M T-6316.1 3533 Table 21 Summary of Urine Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL ------ -------- -------- -------- -------- 0 MEAN S.D. N 26 12.0 10 76 34.6 10 .22 .119 9 .64 .273 9 1 MEAN S.D. N 18 12.0 10 51 41.3 10 .22 .126 8 .56 .333 8 Covance 6329-212 Covance 6329-228 3M T-6316.1 3534 Table 22 Summary of Urine Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL ------ -------- -------- -------- -------- 0 MEAN S.D. N 18 9.3 10 112 52.8 10 .18 .062 10 1.11 .383 10 1 MEAN S.D. N 26 18.7 10 151 123.3 10 .24 .135 9 1.34 .363 9 Covance 6329-212 Covance 6329-228 3M T-6316.1 3535 Table 22 Summary of Urine Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL ------ -------- -------- -------- -------- 0 MEAN S.D. N 16 16.3 10 101 83.6 10 .08 .025 8 .61 .212 8 1 MEAN S.D. N 10 7.1 9 70 47.4 9 .14 * .057 9 .96 * .383 9 Covance 6329-212 Covance 6329-228 3M T-6316.1 3536 Table 23 Summary of Urine Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL ------ -------- -------- -------- -------- 0 MEAN S.D. N 20 13.0 10 143 68.2 10 .23 .135 9 1.61 .579 9 1 MEAN S.D. N 26 17.9 10 165 88.6 10 .20 .100 10 1.29 .256 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3537 Table 23 Summary of Urine Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL ------ -------- -------- -------- -------- 0 MEAN S.D. N 13 9.6 10 64 48.6 10 .25 .191 6 .93 .569 6 1 MEAN S.D. N 20 14.9 10 118 101.1 10 .18 .085 10 .93 .290 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3538 Covance 6329-212 Table 24 Summary of Organ Weight Data Covance 6329-228 3M T-6316.1 Week 4 Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=1;SUBSET=T LIVER -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M8 ------------------------------- NUMBER IN GROUP: 5 5 5 MEAN: 314.0 11.5023 3.6573 STANDARD DEV: 18.3 1.4141 0.3062 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F8 ------------------------------- NUMBER IN GROUP: 5 5 5 MEAN: 199.2 7.7008 3.8600 STANDARD DEV: 14.9 0.9577 0.2780 --------------------------------------------------------------- M9 ------------------------------- NUMBER IN GROUP: 5 5 5 MEAN: 308.7 11.1683 3.6223 STANDARD DEV: 14.1 0.9088 0.3092 --------------------------------------------------------------- F9 ------------------------------- NUMBER IN GROUP: 5 5 5 MEAN: 218.0 8.1095 3.7211 STANDARD DEV: 20.1 1.1330 0.3797 --------------------------------------------------------------- --------------------------------------------------------------- 3539 Covance 6329-212 Table 25 Summary of Organ Weight Data Covance 6329-228 3M T-6316.1 Week 14 Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=T LIVER -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M8 ------------------------------- NUMBER IN GROUP: 5 5 5 MEAN: 501.8 14.4950 2.8829 STANDARD DEV: 59.4 2.0800 0.1069 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F8 ------------------------------- NUMBER IN GROUP: 5 5 5 MEAN: 270.7 8.0939 3.0001 STANDARD DEV: 16.9 0.4382 0.2600 --------------------------------------------------------------- M9 ------------------------------- NUMBER IN GROUP: 5 4 4 MEAN: 525.2 16.6952 3.1146 STANDARD DEV: 47.7 2.7892 0.2426 --------------------------------------------------------------- F9 ------------------------------- NUMBER IN GROUP: 5 5 5 MEAN: 254.9 7.5958 2.9983 STANDARD DEV: 23.0 0.1717 0.2565 --------------------------------------------------------------- --------------------------------------------------------------- 3540 Covance 6329-212 Table 26 Summary of Organ Weight Data Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT ADRENAL -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 721.2 0.0309 0.0043 0.0128 STANDARD DEV: 89.7 0.0067 0.0007 0.0026 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 412.5 0.0525 0.0133 0.0244 STANDARD DEV: 85.7 0.0225 0.0068 0.0115 --------------------------------------------------------------- M9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 725.1 0.0334 0.0046 0.0140 STANDARD DEV: 76.6 0.0053 0.0007 0.0021 --------------------------------------------------------------- F9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 396.4 0.0435 0.0111 0.0212 STANDARD DEV: 49.5 0.0075 0.0022 0.0030 --------------------------------------------------------------- --------------------------------------------------------------- 3541 Covance 6329-212 Table 26 Summary of Organ Weight Data Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT ADRENAL -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 721.2 0.0320 0.0045 0.0132 STANDARD DEV: 89.7 0.0046 0.0006 0.0016 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 412.5 0.0384 0.0096 0.0177 STANDARD DEV: 85.7 0.0086 0.0031 0.0042 --------------------------------------------------------------- M9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 725.1 0.0286 0.0040 0.0120 STANDARD DEV: 76.6 0.0034 0.0007 0.0014 --------------------------------------------------------------- F9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 396.4 0.0379 0.0096 0.0186 STANDARD DEV: 49.5 0.0072 0.0019 0.0036 --------------------------------------------------------------- --------------------------------------------------------------- 3542 Covance 6329-212 Table 26 Summary of Organ Weight Data Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL BRAIN -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 721.2 2.4158 0.3385 1.0000 STANDARD DEV: 89.7 0.1977 0.0420 0.0000 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 412.5 2.1708 0.5433 1.0000 STANDARD DEV: 85.7 0.1447 0.0978 0.0000 --------------------------------------------------------------- M9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 725.1 2.3887 0.3324 1.0000 STANDARD DEV: 76.6 0.0908 0.0345 0.0000 --------------------------------------------------------------- F9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 396.4 2.0441 0.5221 1.0000 STANDARD DEV: 49.5 0.0983 0.0618 0.0000 --------------------------------------------------------------- --------------------------------------------------------------- 3543 Covance 6329-212 Table 26 Summary of Organ Weight Data Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT KIDNEY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 721.2 2.3570 0.3279 0.9754 STANDARD DEV: 89.7 0.2803 0.0270 0.0820 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 412.5 1.4112 0.3461 0.6518 STANDARD DEV: 85.7 0.2048 0.0284 0.0994 --------------------------------------------------------------- M9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 725.1 2.2022 0.3056 0.9215 STANDARD DEV: 76.6 0.3009 0.0435 0.1177 --------------------------------------------------------------- F9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 396.4 1.3218 0.3353 0.6460 STANDARD DEV: 49.5 0.1769 0.0394 0.0730 --------------------------------------------------------------- --------------------------------------------------------------- 3544 Covance 6329-212 Table 26 Summary of Organ Weight Data Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT KIDNEY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 721.2 2.3234 0.3234 0.9622 STANDARD DEV: 89.7 0.2897 0.0315 0.0962 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 412.5 1.4370 0.3507 0.6636 STANDARD DEV: 85.7 0.2720 0.0371 0.1305 --------------------------------------------------------------- M9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 725.1 2.2647 0.3144 0.9474 STANDARD DEV: 76.6 0.3230 0.0491 0.1257 --------------------------------------------------------------- F9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 396.4 1.3601 0.3449 0.6642 STANDARD DEV: 49.5 0.2061 0.0481 0.0832 --------------------------------------------------------------- --------------------------------------------------------------- 3545 Covance 6329-212 Table 26 Summary of Organ Weight Data Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LIVER -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 721.2 20.2343 2.8067 8.3464 STANDARD DEV: 89.7 4.2122 0.4847 1.4431 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 412.5 11.3196 2.7467 5.2305 STANDARD DEV: 85.7 2.4524 0.1596 1.1889 --------------------------------------------------------------- M9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 725.1 20.3650 2.8120 8.5202 STANDARD DEV: 76.6 2.9205 0.3074 1.1115 --------------------------------------------------------------- F9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 396.4 10.9316 2.7642 5.3527 STANDARD DEV: 49.5 1.4863 0.2431 0.7110 --------------------------------------------------------------- --------------------------------------------------------------- 3546 Covance 6329-212 Table 26 Summary of Organ Weight Data Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LUNG -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 721.2 2.1758 0.3044 0.9008 STANDARD DEV: 89.7 0.2190 0.0381 0.0625 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 412.5 1.6720 0.4216 0.7736 STANDARD DEV: 85.7 0.2500 0.1081 0.1347 --------------------------------------------------------------- M9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 725.1 2.0380 0.2845 0.8542 STANDARD DEV: 76.6 0.1979 0.0431 0.0877 --------------------------------------------------------------- F9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 396.4 1.4877 0.3787 0.7284 STANDARD DEV: 49.5 0.1285 0.0404 0.0599 --------------------------------------------------------------- --------------------------------------------------------------- 3547 Covance 6329-212 Table 26 Summary of Organ Weight Data Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT OVARY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M8 ------------------------------- NUMBER IN GROUP: 10 MEAN: 721.2 STANDARD DEV: 89.7 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 412.5 0.0564 0.0143 0.0263 STANDARD DEV: 85.7 0.0161 0.0053 0.0083 --------------------------------------------------------------- M9 ------------------------------- NUMBER IN GROUP: 10 MEAN: 725.1 STANDARD DEV: 76.6 --------------------------------------------------------------- F9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 396.4 0.0611 0.0155 0.0297 STANDARD DEV: 49.5 0.0183 0.0047 0.0081 --------------------------------------------------------------- --------------------------------------------------------------- 3548 Covance 6329-212 Table 26 Summary of Organ Weight Data Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT OVARY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M8 ------------------------------- NUMBER IN GROUP: 10 MEAN: 721.2 STANDARD DEV: 89.7 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 412.5 0.0544 0.0140 0.0253 STANDARD DEV: 85.7 0.0176 0.0059 0.0090 --------------------------------------------------------------- M9 ------------------------------- NUMBER IN GROUP: 10 MEAN: 725.1 STANDARD DEV: 76.6 --------------------------------------------------------------- F9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 396.4 0.0624 0.0160 0.0305 STANDARD DEV: 49.5 0.0105 0.0035 0.0044 --------------------------------------------------------------- --------------------------------------------------------------- 3549 Covance 6329-212 Table 26 Summary of Organ Weight Data Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL SPLEEN -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 721.2 1.0371 0.1430 0.4265 STANDARD DEV: 89.7 0.2467 0.0218 0.0763 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 412.5 0.5884 0.1446 0.2721 STANDARD DEV: 85.7 0.0964 0.0191 0.0491 --------------------------------------------------------------- M9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 725.1 1.1631 0.1618 0.4862 STANDARD DEV: 76.6 0.2368 0.0364 0.0943 --------------------------------------------------------------- F9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 396.4 0.5933 0.1516 0.2911 STANDARD DEV: 49.5 0.1075 0.0313 0.0554 --------------------------------------------------------------- --------------------------------------------------------------- 3550 Covance 6329-212 Table 26 Summary of Organ Weight Data Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT TESTIS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 721.2 1.8105 0.2529 0.7539 STANDARD DEV: 89.7 0.1543 0.0240 0.0873 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F8 ------------------------------- NUMBER IN GROUP: 10 MEAN: 412.5 STANDARD DEV: 85.7 --------------------------------------------------------------- M9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 725.1 1.7120 0.2403 0.7138 STANDARD DEV: 76.6 0.3999 0.0685 0.1556 --------------------------------------------------------------- F9 ------------------------------- NUMBER IN GROUP: 10 MEAN: 396.4 STANDARD DEV: 49.5 --------------------------------------------------------------- --------------------------------------------------------------- 3551 Covance 6329-212 Table 26 Summary of Organ Weight Data Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT TESTIS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 721.2 1.8082 0.2523 0.7536 STANDARD DEV: 89.7 0.1769 0.0226 0.1006 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F8 ------------------------------- NUMBER IN GROUP: 10 MEAN: 412.5 STANDARD DEV: 85.7 --------------------------------------------------------------- M9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 725.1 1.8413 0.2571 0.7703 STANDARD DEV: 76.6 0.2307 0.0479 0.0872 --------------------------------------------------------------- F9 ------------------------------- NUMBER IN GROUP: 10 MEAN: 396.4 STANDARD DEV: 49.5 --------------------------------------------------------------- --------------------------------------------------------------- 3552 Covance 6329-212 Table 26 Summary of Organ Weight Data Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LF THYROID/PARA -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 721.2 0.0232 0.0032 0.0096 STANDARD DEV: 89.7 0.0067 0.0007 0.0026 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 412.5 0.0142 0.0035 0.0066 STANDARD DEV: 85.7 0.0032 0.0007 0.0017 --------------------------------------------------------------- M9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 725.1 0.0202 0.0028 0.0085 STANDARD DEV: 76.6 0.0050 0.0008 0.0021 --------------------------------------------------------------- F9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 396.4 0.0177 0.0045 0.0087 STANDARD DEV: 49.5 0.0066 0.0016 0.0034 --------------------------------------------------------------- --------------------------------------------------------------- 3553 Covance 6329-212 Table 26 Summary of Organ Weight Data Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL RT THYROID/PARA -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 721.2 0.0251 0.0035 0.0104 STANDARD DEV: 89.7 0.0079 0.0009 0.0030 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 412.5 0.0142 0.0035 0.0066 STANDARD DEV: 85.7 0.0025 0.0005 0.0013 --------------------------------------------------------------- M9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 725.1 0.0242 0.0034 0.0101 STANDARD DEV: 76.6 0.0060 0.0009 0.0023 --------------------------------------------------------------- F9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 396.4 0.0147 0.0037 0.0072 STANDARD DEV: 49.5 0.0044 0.0010 0.0020 --------------------------------------------------------------- --------------------------------------------------------------- 3554 Covance 6329-212 Table 26 Summary of Organ Weight Data Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL UTERUS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M8 ------------------------------- NUMBER IN GROUP: 10 MEAN: 721.2 STANDARD DEV: 89.7 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F8 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 412.5 1.0319 0.2563 0.4776 STANDARD DEV: 85.7 0.1956 0.0631 0.1015 --------------------------------------------------------------- M9 ------------------------------- NUMBER IN GROUP: 10 MEAN: 725.1 STANDARD DEV: 76.6 --------------------------------------------------------------- F9 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 396.4 1.0749 0.2753 0.5268 STANDARD DEV: 49.5 0.2526 0.0763 0.1225 --------------------------------------------------------------- --------------------------------------------------------------- 3555 Covance 6329-212 Table 27 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Week 4 Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=1;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- ** TOP OF LIST ** LIVER (LI) ........................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 ** END OF LIST ** 3556 Covance 6329-212 Table 28 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Week 14 Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=2;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- ** TOP OF LIST ** LIVER (LI) ........................................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 ** END OF LIST ** 3557 Covance 6329-212 Table 29 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 10 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ................................. NUMBER EXAMINED: 10 10 10 10 NO MACROSCOPIC LESIONS MALOCCLUSION ANIMAL OBESE BONE MARROW SMEAR TAKEN TEETH MISSING/BROKEN/WORN 1422 0011 7301 10 10 10 10 0010 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 EYE (EY) ............................................. NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 3558 Covance 6329-212 Table 29 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 10 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- BRAIN (BR) ........................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 9 COMPRESSED (HYPOTHALAMUS) 0001 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 LUNG (LU) ............................................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 9 10 LIGHT FOCUS(I)/AREA(S) 0010 KIDNEY (KD) .......................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 9 10 9 LARGE PELVIS(ES) CYST(S) 0100 0001 LIVER (LI) ........................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 8 10 9 10 LIGHT FOCUS(I)/AREA(S) MOTTLED RED FOCUS(I)/AREA(S) 1000 1000 0010 3559 Covance 6329-212 Table 29 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 10 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- HEART (HT) ........................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 SPLEEN (SP) .......................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 9 10 10 LIGHT FOCUS(I)/AREA(S) 0100 THYMUS (TH) .......................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 3560 Covance 6329-212 Table 29 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 10 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- THYROID (TY) ......................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 TRACHEA (TR) ......................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 9 10 LARGE 0010 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 PITUITARY (PI) ....................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 9 5 8 DARK FOCUS(I)/AREA(S) LARGE DIFFUSELY RED ** CONTINUED ON NEXT PAGE ** 0010 0142 0001 3561 Covance 6329-212 Table 29 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 10 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** PITUITARY (PI) ....................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 9 5 8 RED FOCUS(I)/AREA(S) 0021 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 PANCREAS (PA) ........................................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 8 10 DARK FOCUS(I)/AREA(S) 0020 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 3562 Covance 6329-212 Table 29 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 10 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- DUODENUM (DU) ........................................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 ILEUM (IL) ........................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 CECUM (CE) ........................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 COLON (CO) ........................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 RECTUM (RE) .......................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 3563 Covance 6329-212 Table 29 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 10 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- SKIN (SK) ............................................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 9 CRUSTED AREA(S) 0001 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 10 10 065 MASS(ES) CYST(S) 0012 0034 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 10 10 0 10 10 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 CYST(S) 0 OVARY (OV) ........................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 10 10 0 10 9 001 0 10 10 0 10 10 3564 Covance 6329-212 Table 29 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 10 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- VAGINA (VA) .......................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 10 10 0 10 10 PROSTATE (PR) ........................................ NUMBER EXAMINED: 10 10 0 0 NOT REMARKABLE: 10 10 0 0 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 10 10 0 0 NOT REMARKABLE: 10 10 0 0 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 10 10 0 0 NOT REMARKABLE: 10 10 0 0 TESTIS (TE) .......................................... NUMBER EXAMINED: 10 10 0 0 NOT REMARKABLE: 10 9 0 0 SMALL 0100 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 URETER (UE) .......................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 3565 Covance 6329-212 Table 29 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 10 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 9 10 DIFFUSELY RED 0010 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 8 7 DIFFUSELY RED MOTTLED 0022 0001 ADRENAL (AD) ......................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 3566 Covance 6329-212 Table 29 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 10 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- TAIL (TI) ............................................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 10 10 0 0 NOT REMARKABLE: 10 10 0 0 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 AORTA (AO) ........................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 3567 Covance 6329-212 Table 29 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 10 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 ** END OF LIST ** 3568 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 11 20 13 15 ------------------------------------------------------------------------ -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ................................. NUMBER EXAMINED: 11 20 13 15 NO MACROSCOPIC LESIONS BLOOD FILM TAKEN MALOCCLUSION STAINS-PERINEUM/PERIANAL ANIMAL OBESE DISCHARGE-OCULAR ANIMAL THIN BONE MARROW SMEAR TAKEN TEETH MISSING/BROKEN/WORN STAINS - SKIN/HAIRCOAT 0100 11 20 13 14 1111 3100 1000 2001 3110 11 20 13 14 1011 0021 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 3569 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 11 20 13 15 ------------------------------------------------------------------------ -=- -=- -=- -=- BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 EYE (EY) ............................................. NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 19 13 15 WHITE INTRAOCULAR MATERIAL 0100 BRAIN (BR) ........................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 7 18 8 5 COMPRESSED (HYPOTHALAMUS) 4 2 5 10 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 LUNG (LU) ............................................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 19 13 14 LIGHT FOCUS(I)/AREA(S) MOTTLED 0001 0100 3570 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 11 20 13 15 ------------------------------------------------------------------------ -=- -=- -=- -=- KIDNEY (KD) .......................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 8 16 13 15 LARGE PELVIS(ES) ROUGH SURFACE, DIFFUSE MASS(ES) GRANULAR MATERIAL DIFFUSELY LIGHT LARGE 1200 1200 1000 1100 1300 0200 LIVER (LI) ........................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 6 9 6 6 LIGHT FOCUS(I)/AREA(S) MOTTLED RED FOCUS(I)/AREA(S) DIFFUSELY LIGHT MASS(ES) LARGE RAISED FOCUS(I)/AREA(S) CYST(S) DARK FOCUS(I)/AREA(S) 0220 0110 4734 0100 1100 0110 0001 0001 0103 HEART (HT) ........................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 12 15 LIGHT FOCUS(I)/AREA(S) 0010 3571 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 11 20 13 15 ------------------------------------------------------------------------ -=- -=- -=- -=- MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 14 MASS(ES) 0001 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 18 13 15 DIFFUSELY RED LARGE 0200 0100 SPLEEN (SP) .......................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 17 12 14 LARGE RAISED FOCUS(I)/AREA(S) 0111 0200 THYMUS (TH) .......................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 12 15 MASS(ES) 0010 3572 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 11 20 13 15 ------------------------------------------------------------------------ -=- -=- -=- -=- ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 THYROID (TY) ......................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 10 19 13 15 LARGE 1100 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 10 20 13 14 LARGE 1001 TRACHEA (TR) ......................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 8 12 LARGE DARK FOCUS(I)/AREA(S) MASS(ES) 0023 0010 0020 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 3573 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 11 20 13 15 ------------------------------------------------------------------------ -=- -=- -=- -=- PITUITARY (PI) ....................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 4 11 2 3 MASS(ES) LARGE DIFFUSELY RED RED FOCUS(I)/AREA(S) RAISED AREA(S) CYST(S) 5 2 7 10 1021 0110 1511 1010 0100 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 PANCREAS (PA) ........................................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 10 18 12 15 LARGE VESSELS MASS(ES) 1000 0210 3574 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 11 20 13 15 ------------------------------------------------------------------------ -=- -=- -=- -=- STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 7 14 12 14 DARK FOCUS(I)/AREA(S) EROSION/ULCERATION THICKENED WALL 1310 2000 1401 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 19 13 15 EROSION/ULCERATION 0100 DUODENUM (DU) ........................................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 12 15 DIVERTICULUM(A) 0010 ILEUM (IL) ........................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 CECUM (CE) ........................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 3575 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 11 20 13 15 ------------------------------------------------------------------------ -=- -=- -=- -=- COLON (CO) ........................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 RECTUM (RE) .......................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 SKIN (SK) ............................................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 7 16 11 12 CRUSTED AREA(S) MASS(ES) ABRASION(S)/ULCERATION(S) RAISED AREA(S) 2212 1101 0110 1100 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 13 15 068 MASS(ES) THICKENED MASS #2 MASS #3 0077 0021 0044 0010 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 3576 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 11 20 13 15 ------------------------------------------------------------------------ -=- -=- -=- -=- CERVIX (CV) .......................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 13 15 0 13 14 LARGE 0001 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 13 15 0 11 13 CYST(S) MASS(ES) 0012 0010 OVARY (OV) ........................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 13 15 0 8 10 CYST(S) MASS(ES) 0055 0010 VAGINA (VA) .......................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 13 15 0 13 15 PROSTATE (PR) ........................................ NUMBER EXAMINED: 11 20 0 0 NOT REMARKABLE: 11 20 0 0 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 11 20 0 0 NOT REMARKABLE: 11 20 0 0 3577 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 11 20 13 15 ------------------------------------------------------------------------ -=- -=- -=- -=- EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 11 20 0 0 NOT REMARKABLE: 11 20 0 0 TESTIS (TE) .......................................... NUMBER EXAMINED: 11 20 0 0 NOT REMARKABLE: 8 16 0 0 SMALL CONTAINS FLUID RED/YELLOW FOCUS(I)/AREA(S) 3300 1000 0200 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 URETER (UE) .......................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 19 12 14 LARGE ** CONTINUED ON NEXT PAGE ** 0110 3578 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 11 20 13 15 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 19 12 14 MOTTLED 0111 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 12 15 MOTTLED 0010 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 11 14 DIFFUSELY RED MOTTLED LARGE 0010 0010 0011 ADRENAL (AD) ......................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 8 18 11 12 CRUSTED AREA(S) ABRASION(S)/ULCERATION(S) 3020 0203 3579 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 11 20 13 15 ------------------------------------------------------------------------ -=- -=- -=- -=- TAIL (TI) ............................................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 18 13 14 RAISED AREA(S) ULCERATION(S)/ABRASION(S) 0200 0001 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 11 20 0 0 NOT REMARKABLE: 10 20 0 0 MASS(ES) 1000 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 10 19 13 14 LARGE 1101 AORTA (AO) ........................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 19 13 15 LARGE 0100 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 10 19 13 15 LARGE DIFFUSELY RED 1100 1100 3580 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 11 20 13 15 ------------------------------------------------------------------------ -=- -=- -=- -=- LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 12 15 LARGE 0010 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 19 13 15 LARGE 0100 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 3581 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 11 20 13 15 ------------------------------------------------------------------------ -=- -=- -=- -=- LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 18 13 15 LARGE DIFFUSELY RED 0100 0100 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 9 20 13 14 LARGE 2001 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 12 15 LARGE MOTTLED ** END OF LIST ** 0010 0010 3582 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ................................. NUMBER EXAMINED: 39 30 37 35 BLOOD FILM TAKEN MALOCCLUSION STAINS-PERINEUM/PERIANAL URINARY BLADDER-LARGE LUMEN AUTOLYSIS-SEVERE ANIMAL OBESE GI TRACT-LIGHT FLUID CONTENT GI TRACT-LARGE LUMEN DISCHARGE-OCULAR DISCHARGE-NASAL ANIMAL THIN TEETH MISSING/BROKEN/WORN GI TRACT-DARK FLUID CONTENT STAINS - SKIN/HAIRCOAT DISCHARGE-ORAL FLUID-THORACIC CAVITY 29 19 28 30 4475 12 5 8 7 3200 2000 4312 0010 0110 12 8 19 14 7 5 10 5 7 8 12 11 0221 1120 6020 0110 1000 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 3583 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 36 35 MISALIGNED 0010 EYE (EY) ............................................. NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 29 37 35 OPAQUE CORNEA 0100 BRAIN (BR) ........................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 18 14 5 6 COMPRESSED (HYPOTHALAMUS) 21 16 32 29 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 LUNG (LU) ............................................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 38 26 36 32 LIGHT FOCUS(I)/AREA(S) ** CONTINUED ON NEXT PAGE ** 0110 3584 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LUNG (LU) ............................................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 38 26 36 32 UNCOLLAPSED RED FOCUS(I)/AREA(S) ADHESION(S) DIFFUSELY RED 0203 0001 0200 1100 KIDNEY (KD) .......................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 25 19 33 33 LARGE PELVIS(ES) LIGHT FOCUS(I)/AREA(S) ROUGH SURFACE, DIFFUSE MASS(ES) CYST(S) GRANULAR MATERIAL DIFFUSELY LIGHT DIFFUSELY DARK LARGE MOTTLED 8511 0010 4800 1000 1100 1112 7501 0010 2200 0010 LIVER (LI) ........................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 21 19 27 28 LIGHT FOCUS(I)/AREA(S) MOTTLED ** CONTINUED ON NEXT PAGE ** 1512 3021 3585 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 21 19 27 28 RED FOCUS(I)/AREA(S) DIFFUSELY LIGHT MASS(ES) LARGE DEPRESSED FOCUS(I)/AREA(S) RAISED FOCUS(I)/AREA(S) CYST(S) DARK FOCUS(I)/AREA(S) ROUGH SURFACE, DIFFUSE 4241 4321 2200 3201 1010 1001 1001 0100 1000 HEART (HT) ........................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 37 27 37 34 LIGHT FOCUS(I)/AREA(S) MASS(ES) 1301 1000 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 29 37 35 MASS(ES) 0100 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 3586 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 29 36 35 DIFFUSELY RED LARGE 0010 0100 SPLEEN (SP) .......................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 36 26 37 32 LIGHT FOCUS(I)/AREA(S) DIFFUSELY LIGHT LARGE ROUGH SURFACE DEPRESSED FOCUS(I)/AREA(S) MASS(ES) 0100 0001 2102 1000 0100 0100 THYMUS (TH) .......................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 29 37 34 LARGE 0101 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 3587 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- THYROID (TY) ......................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 38 27 37 34 LARGE MASS(ES) DIFFUSELY RED 1200 0100 0001 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 37 26 37 35 LARGE 2400 TRACHEA (TR) ......................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 36 25 31 27 LARGE LIGHT FOCUS (I)/AREA(S) SMALL MISSING/NOT IDENTIFIED DIFFUSELY RED MOTTLED DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) ** CONTINUED ON NEXT PAGE ** 0448 0010 0010 1000 1000 0012 0100 1000 3588 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 36 25 31 27 DIFFUSELY LIGHT 0100 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 PITUITARY (PI) ....................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 11 13 2 4 MASS(ES) DARK FOCUS(I)/AREA(S) LARGE DIFFUSELY RED RED FOCUS(I)/AREA(S) RAISED AREA(S) CYST(S) 20 16 31 29 0020 2012 1000 1110 3000 1010 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 3589 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- PANCREAS (PA) ........................................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 26 36 35 LARGE VESSELS MASS(ES) 0200 0210 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 23 19 35 33 RED FOCUS(I)/AREA(S) DARK FOCUS(I)/AREA(S) LIGHT FOCUS(I)/AREA(S) EROSION/ULCERATION THICKENED WALL ADHESION(S) 4010 3812 1100 2100 8200 0100 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 31 27 32 32 THICKENED WALL EROSION/ULCERATION DARK FOCUS(I)/AREA(S) 6231 5142 0100 DUODENUM (DU) ........................................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 37 30 37 35 RED FOCUS(I)/AREA(S) ** CONTINUED ON NEXT PAGE ** 1000 3590 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** DUODENUM (DU) ........................................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 37 30 37 35 THICKENED WALL 1000 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 38 30 37 35 RED FOCUS(I)/AREA(S) 1000 ILEUM (IL) ........................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 36 35 LARGE LYMPHOID AREA(S) 0010 CECUM (CE) ........................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 38 29 37 32 THICKENED WALL MASS(ES) RED FOCUS(I)/AREA(S) 1102 0002 1000 COLON (CO) ........................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 3591 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- RECTUM (RE) .......................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 SKIN (SK) ............................................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 35 24 36 34 CRUSTED AREA(S) MASS(ES) SUBCUTANEOUS EDEMA ALOPECIA-DIFFUSE 3510 0101 1000 0100 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 37 35 0 11 13 MASS(ES) CYST(S) THICKENED MASS #2 MASS #3 0 0 19 18 0077 0 0 10 9 0 0 11 9 0043 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 37 28 36 35 CALCULUS(I) THICKENED WALL RED FOCUS(I)/AREA(S) ** CONTINUED ON NEXT PAGE ** 2010 2000 1000 3592 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 37 28 36 35 DIFFUSELY RED SEMIFLUID MATERIAL 1200 1000 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 37 35 0 36 34 LARGE 0011 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 37 35 0 32 28 CYST(S) MASS(ES) SMALL THICKENED WALL 0046 0002 0010 0001 OVARY (OV) ........................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 37 35 0 31 28 DIFFUSELY RED CYST(S) 0010 0057 3593 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- VAGINA (VA) .......................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 37 35 0 33 27 SEMIFLUID MATERIAL THICKENED WALL 0048 0001 PROSTATE (PR) ........................................ NUMBER EXAMINED: 39 30 0 0 NOT REMARKABLE: 30 20 0 0 GELATINOUS NODULAR MASS(ES) LARGE SMALL DIFFUSELY RED 1000 7600 0100 1100 0200 1000 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 39 30 0 0 NOT REMARKABLE: 31 25 0 0 GELATINOUS MASS(ES) LARGE SMALL 1200 1000 1200 5100 3594 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 39 30 0 0 NOT REMARKABLE: 39 28 0 0 MASS(ES) SMALL 0100 0100 TESTIS (TE) .......................................... NUMBER EXAMINED: 39 30 0 0 NOT REMARKABLE: 34 29 0 0 SMALL 5100 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 36 35 ABRASION(S)/ULCERATION(S) 0010 URETER (UE) .......................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 37 30 37 35 LARGE 2000 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 38 29 36 35 FRACTURE RED FOCUS(I)/AREA(S) MASS(ES) 0010 1000 0100 3595 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 32 31 LARGE DIFFUSELY RED MOTTLED 0042 0022 0001 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 38 30 37 34 DIFFUSELY RED LARGE 1001 1000 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 36 29 33 32 DIFFUSELY RED MOTTLED LARGE 2011 1011 0121 ADRENAL (AD) ......................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 3596 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 31 26 37 32 CRUSTED AREA(S) ABRASION(S)/ULCERATION(S) LARGE 4202 4200 0001 TAIL (TI) ............................................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 37 29 37 35 CRUSTED AREA(S) RAISED AREA(S) 1100 1000 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 39 30 0 0 NOT REMARKABLE: 32 26 0 0 THICKENED MASS(ES) CYST(S) 0100 5200 2100 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 36 24 37 35 MASS(ES) LARGE DIFFUSELY DARK CYST(S) ** CONTINUED ON NEXT PAGE ** 0100 1300 0100 1100 3597 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 36 24 37 35 MOTTLED 1000 AORTA (AO) ........................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 38 29 37 35 LARGE FIRM 1100 1100 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 28 37 35 LARGE DIFFUSELY RED 0200 0200 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 36 35 LARGE 0010 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 29 37 35 MASS(ES) 0100 3598 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 29 37 35 MASS(ES) 0100 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 29 37 35 LARGE 0100 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 29 37 34 MASS(ES) THICKENED 0100 0001 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 29 37 35 LARGE 0100 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 29 37 35 LARGE 0100 3599 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 ** END OF LIST ** 3600 Covance 6329-212 Table 32 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ................................. NUMBER EXAMINED: 70 70 70 70 NO MACROSCOPIC LESIONS BLOOD FILM TAKEN MALOCCLUSION STAINS-PERINEUM/PERIANAL URINARY BLADDER-LARGE LUMEN AUTOLYSIS-SEVERE ANIMAL OBESE GI TRACT-LIGHT FLUID CONTENT GI TRACT-LARGE LUMEN DISCHARGE-OCULAR DISCHARGE-NASAL ANIMAL THIN BONE MARROW SMEAR TAKEN TEETH MISSING/BROKEN/WORN GI TRACT-DARK FLUID CONTENT STAINS - SKIN/HAIRCOAT DISCHARGE-ORAL FLUID-THORACIC CAVITY 11 15 12 12 40 39 41 44 5597 15 6 8 7 3200 2000 12 6 1 3 0010 0110 14 8 19 15 7 5 10 5 10 9 13 11 21 30 23 24 1242 1120 6041 0110 1000 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 3601 Covance 6329-212 Table 32 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 69 70 MISALIGNED 0010 EYE (EY) ............................................. NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 68 70 70 OPAQUE CORNEA WHITE INTRAOCULAR MATERIAL 0100 0100 BRAIN (BR) ........................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 45 52 33 30 COMPRESSED (HYPOTHALAMUS) 25 18 37 40 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 3602 Covance 6329-212 Table 32 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- LUNG (LU) ............................................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 69 65 68 66 LIGHT FOCUS(I)/AREA(S) UNCOLLAPSED RED FOCUS(I)/AREA(S) ADHESION(S) DIFFUSELY RED MOTTLED 0121 0203 0001 0200 1100 0100 KIDNEY (KD) .......................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 53 54 66 67 LARGE PELVIS(ES) LIGHT FOCUS(I)/AREA(S) ROUGH SURFACE, DIFFUSE MASS(ES) CYST(S) GRANULAR MATERIAL DIFFUSELY LIGHT DIFFUSELY DARK LARGE MOTTLED 9811 0010 5 10 0 0 2000 1101 2212 8801 0010 2400 0010 3603 Covance 6329-212 Table 32 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- LIVER (LI) ........................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 45 48 52 54 LIGHT FOCUS(I)/AREA(S) MOTTLED RED FOCUS(I)/AREA(S) DIFFUSELY LIGHT MASS(ES) LARGE DEPRESSED FOCUS(I)/AREA(S) RAISED FOCUS(I)/AREA(S) CYST(S) DARK FOCUS(I)/AREA(S) ROUGH SURFACE, DIFFUSE 2732 4131 8985 4421 3300 3311 1010 1002 1002 0203 1000 HEART (HT) ........................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 68 67 69 69 LIGHT FOCUS(I)/AREA(S) MASS(ES) 1311 1000 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 69 70 69 MASS(ES) 0101 3604 Covance 6329-212 Table 32 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 67 69 70 DIFFUSELY RED LARGE 0210 0200 SPLEEN (SP) .......................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 67 62 69 66 LIGHT FOCUS(I)/AREA(S) DIFFUSELY LIGHT LARGE ROUGH SURFACE DEPRESSED FOCUS(I)/AREA(S) MASS(ES) RAISED FOCUS(I)/AREA(S) 0200 0001 2213 1000 0100 0100 0200 THYMUS (TH) .......................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 69 69 69 LARGE MASS(ES) 0101 0010 3605 Covance 6329-212 Table 32 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 THYROID (TY) ......................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 68 66 70 69 LARGE MASS(ES) DIFFUSELY RED 2300 0100 0001 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 67 66 70 69 LARGE 3401 TRACHEA (TR) ......................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 67 65 58 59 LARGE LIGHT FOCUS (I)/AREA(S) SMALL MISSING/NOT IDENTIFIED ** CONTINUED ON NEXT PAGE ** 0 4 7 11 0010 0010 1000 3606 Covance 6329-212 Table 32 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 67 65 58 59 DIFFUSELY RED MOTTLED DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) DIFFUSELY LIGHT MASS(ES) 1000 0012 0110 1000 0100 0020 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 PITUITARY (PI) ....................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 35 43 19 25 MASS(ES) DARK FOCUS(I)/AREA(S) LARGE DIFFUSELY RED RED FOCUS(I)/AREA(S) RAISED AREA(S) CYST(S) 25 18 38 39 0030 3175 1111 2642 4010 1110 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 3607 Covance 6329-212 Table 32 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 PANCREAS (PA) ........................................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 69 64 68 70 LARGE VESSELS MASS(ES) 1200 0420 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 50 53 65 67 RED FOCUS(I)/AREA(S) DARK FOCUS(I)/AREA(S) LIGHT FOCUS(I)/AREA(S) EROSION/ULCERATION THICKENED WALL ADHESION(S) 4010 4 11 4 2 1100 4100 9601 0100 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 62 66 65 67 THICKENED WALL EROSION/ULCERATION DARK FOCUS(I)/AREA(S) 6231 5242 0100 3608 Covance 6329-212 Table 32 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- DUODENUM (DU) ........................................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 68 70 70 70 RED FOCUS(I)/AREA(S) THICKENED WALL 1000 1000 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 69 70 69 70 RED FOCUS(I)/AREA(S) DIVERTICULUM(A) 1000 0010 ILEUM (IL) ........................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 69 70 LARGE LYMPHOID AREA(S) 0010 CECUM (CE) ........................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 69 69 70 67 THICKENED WALL MASS(ES) RED FOCUS(I)/AREA(S) 1102 0002 1000 COLON (CO) ........................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 3609 Covance 6329-212 Table 32 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- RECTUM (RE) .......................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 SKIN (SK) ............................................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 62 60 67 65 CRUSTED AREA(S) MASS(ES) SUBCUTANEOUS EDEMA ALOPECIA-DIFFUSE ABRASION(S)/ULCERATION(S) RAISED AREA(S) 5723 1202 1000 0100 0110 1100 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 70 70 0 33 36 MASS(ES) CYST(S) THICKENED MASS #2 MASS #3 0 0 27 27 0 0 10 11 0 0 12 10 0 0 15 13 0053 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 68 68 69 70 CALCULUS(I) ** CONTINUED ON NEXT PAGE ** 2010 3610 Covance 6329-212 Table 32 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 68 68 69 70 THICKENED WALL RED FOCUS(I)/AREA(S) DIFFUSELY RED SEMIFLUID MATERIAL 2000 1000 1200 1000 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 70 70 0 69 68 LARGE 0012 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 70 70 0 63 60 CYST(S) MASS(ES) SMALL THICKENED WALL 0059 0012 0010 0001 OVARY (OV) ........................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 70 70 0 59 58 DIFFUSELY RED CYST(S) MASS(ES) 0010 0 0 10 12 0010 3611 Covance 6329-212 Table 32 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- VAGINA (VA) .......................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 70 70 0 66 62 SEMIFLUID MATERIAL THICKENED WALL 0048 0001 PROSTATE (PR) ........................................ NUMBER EXAMINED: 70 70 0 0 NOT REMARKABLE: 61 60 0 0 GELATINOUS NODULAR MASS(ES) LARGE SMALL DIFFUSELY RED 1000 7600 0100 1100 0200 1000 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 70 70 0 0 NOT REMARKABLE: 62 65 0 0 GELATINOUS MASS(ES) LARGE SMALL 1200 1000 1200 5100 3612 Covance 6329-212 Table 32 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 70 70 0 0 NOT REMARKABLE: 70 68 0 0 MASS(ES) SMALL 0100 0100 TESTIS (TE) .......................................... NUMBER EXAMINED: 70 70 0 0 NOT REMARKABLE: 62 64 0 0 SMALL CONTAINS FLUID RED/YELLOW FOCUS(I)/AREA(S) 8500 1000 0200 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 69 70 ABRASION(S)/ULCERATION(S) 0010 URETER (UE) .......................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 68 70 70 70 LARGE 2000 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 69 69 69 70 FRACTURE ** CONTINUED ON NEXT PAGE ** 0010 3613 Covance 6329-212 Table 32 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 69 69 69 70 RED FOCUS(I)/AREA(S) MASS(ES) 1000 0100 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 69 63 65 LARGE DIFFUSELY RED MOTTLED 0152 0032 0112 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 69 70 69 69 DIFFUSELY RED LARGE MOTTLED 1001 1000 0010 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 67 69 62 63 DIFFUSELY RED MOTTLED LARGE 2043 1022 0132 3614 Covance 6329-212 Table 32 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- ADRENAL (AD) ......................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 59 64 68 64 CRUSTED AREA(S) ABRASION(S)/ULCERATION(S) LARGE 7222 4403 0001 TAIL (TI) ............................................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 68 67 70 69 CRUSTED AREA(S) RAISED AREA(S) ULCERATION(S)/ABRASION(S) 1100 1200 0001 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 70 70 0 0 NOT REMARKABLE: 62 66 0 0 THICKENED MASS(ES) CYST(S) 0100 6200 2100 3615 Covance 6329-212 Table 32 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 66 63 70 69 MASS(ES) LARGE DIFFUSELY DARK CYST(S) MOTTLED 0100 2401 0100 1100 1000 AORTA (AO) ........................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 69 68 70 70 LARGE FIRM 1200 1100 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 69 67 70 70 LARGE DIFFUSELY RED 1300 1300 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 68 70 LARGE 0020 3616 Covance 6329-212 Table 32 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 69 70 70 MASS(ES) 0100 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 69 70 70 MASS(ES) 0100 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 68 70 70 LARGE 0200 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 69 70 69 MASS(ES) THICKENED 0100 0001 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 69 70 70 LARGE 0100 3617 Covance 6329-212 Table 32 Incidence of Macroscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 67 70 70 LARGE DIFFUSELY RED 0200 0100 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 68 70 70 69 LARGE 2001 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 69 70 LARGE MOTTLED ** END OF LIST ** 0010 0010 3618 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- ** TOP OF LIST ** LUNG (LU) ............................................ NUMBER EXAMINED: 10 10 NOT REMARKABLE: 10 9 --INFILTRATE, MACROPHAGE, ALVEOLAR 01 KIDNEY (KD) .......................................... NUMBER EXAMINED: 10 10 NOT REMARKABLE: 5 1 --CAST, PROTEINACEOUS --MINERALIZATION, PELVIC --NEPHROPATHY, CHRONIC PROGRESSIVE 32 12 26 LIVER (LI) ........................................... NUMBER EXAMINED: 10 10 NOT REMARKABLE: 7 6 --DEGENERATION, CYSTIC --HEMORRHAGE --INFLAMMATION, CHRONIC --LIPIDOSIS, SUBCAPSULAR (TENSION --NECROSIS, HEPATOCELLULAR --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR LIPIDOSIS) 01 20 12 10 10 02 01 PANCREAS (PA) ........................................ NUMBER EXAMINED: 10 10 NOT REMARKABLE: 10 6 --PIGMENT --B-ADENOMA, ISLET CELL 03 01 3619 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- THYROID (TY) ......................................... NUMBER EXAMINED: 10 10 NOT REMARKABLE: 8 6 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL 23 01 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 TESTIS (TE) .......................................... NUMBER EXAMINED: 10 10 NOT REMARKABLE: 10 9 --ATROPHY/DEGENERATION 01 SKIN (SK) ............................................ NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 3620 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 EYE (EY) ............................................. NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 BRAIN (BR) ........................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 3621 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 HEART (HT) ........................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 9 0 --DEGENERATION/NECROSIS 10 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 SPLEEN (SP) .......................................... NUMBER EXAMINED: 10 1 NOT REMARKABLE: 10 1 THYMUS (TH) .......................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 TRACHEA (TR) ......................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 3622 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 PITUITARY (PI) ....................................... NUMBER EXAMINED: 9 1 NOT REMARKABLE: 8 0 --HEMORRHAGE --B-ADENOMA 01 11 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 10 0 NOT REMARKABLE: 9 0 --ONE EXAMINED 10 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 9 0 --EDEMA 10 3623 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 DUODENUM (DU) ........................................ NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 ILEUM (IL) ........................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 CECUM (CE) ........................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 COLON (CO) ........................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 RECTUM (RE) .......................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 9 0 --PARASITISM 10 3624 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 OVARY (OV) ........................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 PROSTATE (PR) ........................................ NUMBER EXAMINED: 10 0 NOT REMARKABLE: 8 0 --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE 10 10 3625 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 DEATH COMMENT (DC) ................................... NUMBER EXAMINED: 10 10 NOT REMARKABLE: 0 0 --SCHEDULED SACRIFICE 10 10 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 3626 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- AORTA (AO) ........................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 HEMATO NEOPLASIA (HN) ................................ NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 TAIL (TI) ............................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 3627 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- URETER (UE) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 3628 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 NASAL TURBINATE (NT) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 3629 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- ** TOP OF LIST ** LUNG (LU) ............................................ NUMBER EXAMINED: 10 10 NOT REMARKABLE: 7 9 --HEMORRHAGE --INFLAMMATION, CHRONIC --INFILTRATE, MACROPHAGE, ALVEOLAR --MINERALIZATION, VASCULAR 10 01 10 10 KIDNEY (KD) .......................................... NUMBER EXAMINED: 10 10 NOT REMARKABLE: 5 5 --CAST, PROTEINACEOUS --CYST --HYPERPLASIA, TRANSITIONAL --INFLAMMATION, CHRONIC --MINERALIZATION, PELVIC CELL 23 11 01 01 44 LIVER (LI) ........................................... NUMBER EXAMINED: 10 10 NOT REMARKABLE: 9 9 --HEMORRHAGE --HYPERPLASIA, BILE DUCT --NECROSIS, HEPATOCELLULAR 10 01 10 PANCREAS (PA) ........................................ NUMBER EXAMINED: 9 10 NOT REMARKABLE: 9 8 --ATROPHY, ACINAR 02 3630 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- THYROID (TY) ......................................... NUMBER EXAMINED: 10 10 NOT REMARKABLE: 8 8 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --INFLAMMATION, CHRONIC 01 21 10 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 TESTIS (TE) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 SKIN (SK) ............................................ NUMBER EXAMINED: 10 1 NOT REMARKABLE: 10 0 --EROSION --INFLAMMATION, ACUTE 01 01 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 10 10 NOT REMARKABLE: 6 4 --FIBROSIS --GALACTOCELE --HEMORRHAGE --MINERALIZATION 01 36 01 11 3631 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 10 10 NOT REMARKABLE: 6 4 --B-FIBROADENOMA 11 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 EYE (EY) ............................................. NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 3632 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- BRAIN (BR) ........................................... NUMBER EXAMINED: 10 1 NOT REMARKABLE: 10 0 --COMPRESSION, VENTRAL 01 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 HEART (HT) ........................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 10 0 NOT REMARKABLE: 9 0 --EDEMA 10 3633 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- SPLEEN (SP) .......................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 THYMUS (TH) .......................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 9 0 --CYST 10 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 TRACHEA (TR) ......................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 10 0 NOT REMARKABLE: 3 0 --ANGIECTASIS --DEGENERATION, CYSTIC, CORTICAL --HEMORRHAGE --ONE EXAMINED 60 30 60 10 3634 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 10 0 NOT REMARKABLE: 8 0 --ONE EXAMINED --B-PHEOCHROMOCYTOMA 10 10 PITUITARY (PI) ....................................... NUMBER EXAMINED: 10 2 NOT REMARKABLE: 6 0 --HEMORRHAGE --PIGMENT --B-ADENOMA 30 10 42 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 10 0 NOT REMARKABLE: 9 0 --INFLAMMATION, CHRONIC 10 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 9 0 --EDEMA 10 3635 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 DUODENUM (DU) ........................................ NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 ILEUM (IL) ........................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 CECUM (CE) ........................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 9 0 --EDEMA 10 COLON (CO) ........................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 3636 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- RECTUM (RE) .......................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 8 0 --PARASITISM 20 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 UTERUS (UT) .......................................... NUMBER EXAMINED: 10 1 NOT REMARKABLE: 8 0 --DILATATION --HYPERPLASIA, CYSTIC, ENDOMETRIAL --INFLAMMATION, ACUTE 10 11 10 OVARY (OV) ........................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 CERVIX (CV) .......................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 VAGINA (VA) .......................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 3637 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 20 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- PROSTATE (PR) ........................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 DEATH COMMENT (DC) ................................... NUMBER EXAMINED: 10 10 NOT REMARKABLE: 0 0 --SCHEDULED SACRIFICE 10 10 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 2 3 NOT REMARKABLE: 0 1 --HEMORRHAGE 22 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 1 1 NOT REMARKABLE: 0 1 --HEMORRHAGE --INFILTRATE, MACROPHAGE, VACUOLATED 10 10 3638 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 21 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 AORTA (AO) ........................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 HEMATO NEOPLASIA (HN) ................................ NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 3639 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 22 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 TAIL (TI) ............................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 URETER (UE) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 3640 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 23 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ------------------------------------------------------------------------ -=- -=- BILE DUCT (BD) ....................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 NASAL TURBINATE (NT) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 3641 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 11 20 ------------------------------------------------------------------------ -=- -=- ** TOP OF LIST ** LUNG (LU) ............................................ NUMBER EXAMINED: 11 20 NOT REMARKABLE: 11 15 --HEMORRHAGE --INFILTRATE, MACROPHAGE, ALVEOLAR --MINERALIZATION, VASCULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 01 02 03 01 KIDNEY (KD) .......................................... NUMBER EXAMINED: 11 20 NOT REMARKABLE: 0 0 --CAST, PROTEINACEOUS --CYST --DILATATION, PELVIC --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFARCT --MINERALIZATION, PELVIC --NEPHROPATHY, CHRONIC PROGRESSIVE --PYELONEPHRITIS --M-CARCINOMA, TUBULAR CELL 3 10 12 13 10 10 02 33 8 10 10 10 LIVER (LI) ........................................... NUMBER EXAMINED: 11 20 NOT REMARKABLE: 1 4 --DEGENERATION, CYSTIC --FIBROSIS, CAPSULAR --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC 24 01 01 3642 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 11 20 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 11 20 NOT REMARKABLE: 1 4 --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC --HEMORRHAGE --HYPERPLASIA, BILE DUCT --INFLAMMATION, CHRONIC --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 16 11 45 6 10 05 11 02 PANCREAS (PA) ........................................ NUMBER EXAMINED: 11 20 NOT REMARKABLE: 3 10 --ATROPHY, ACINAR --FIBROSIS --HEMORRHAGE --HYPERPLASIA, ISLET CELLS --HYPERTROPHY, VASCULAR --INFLAMMATION, VASCULAR --PIGMENT --B-ADENOMA, ACINAR CELL --B-ADENOMA, ISLET CELL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 55 01 11 10 20 10 12 01 25 01 3643 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 11 20 ------------------------------------------------------------------------ -=- -=- THYROID (TY) ......................................... NUMBER EXAMINED: 11 20 NOT REMARKABLE: 5 13 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --FIBROSIS --HYPERPLASIA, C-CELL --HYPERPLASIA, FOLLICULAR CELL --INFLAMMATION, CHRONIC --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL 32 24 11 10 01 11 02 11 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 11 0 NOT REMARKABLE: 10 0 --HYPERPLASIA 10 TESTIS (TE) .......................................... NUMBER EXAMINED: 11 20 NOT REMARKABLE: 6 11 --ATROPHY/DEGENERATION --CYST --INFLAMMATION, CHRONIC --MINERALIZATION --MINERALIZATION, VASCULAR --B-INTERSTITIAL CELL TUMOR 44 01 23 04 01 03 3644 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 11 20 ------------------------------------------------------------------------ -=- -=- SKIN (SK) ............................................ NUMBER EXAMINED: 11 4 NOT REMARKABLE: 7 0 --ATROPHY, ADNEXAL --CYST, EPIDERMAL INCLUSION --EROSION --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --B-ADENOMA, BASAL CELL --B-FIBROMA --B-PAPILLOMA, SQUAMOUS CELL --M-CARCINOMA, SQUAMOUS CELL 01 11 02 01 10 21 01 11 02 10 10 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 11 0 NOT REMARKABLE: 11 0 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 11 0 NOT REMARKABLE: 11 0 3645 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 11 20 ------------------------------------------------------------------------ -=- -=- MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 11 0 NOT REMARKABLE: 11 0 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 11 0 NOT REMARKABLE: 11 0 EYE (EY) ............................................. NUMBER EXAMINED: 11 1 NOT REMARKABLE: 11 0 --CATARACT 01 BRAIN (BR) ........................................... NUMBER EXAMINED: 11 2 NOT REMARKABLE: 4 0 --COMPRESSION, VENTRAL --DILATATION, VENTRICULAR --INFLAMMATION, CHRONIC --MINERALIZATION 42 30 10 20 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 11 0 NOT REMARKABLE: 11 0 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 11 0 NOT REMARKABLE: 11 0 3646 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 11 20 ------------------------------------------------------------------------ -=- -=- NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 HEART (HT) ........................................... NUMBER EXAMINED: 11 0 NOT REMARKABLE: 3 0 --DEGENERATION/NECROSIS --FIBROSIS 80 80 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 11 2 NOT REMARKABLE: 11 1 --HEMORRHAGE --N-HEMANGIOSARCOMA 01 01 SPLEEN (SP) .......................................... NUMBER EXAMINED: 11 3 NOT REMARKABLE: 11 1 --B-LEIOMYOMA --M-HEMANGIOSARCOMA 01 01 THYMUS (TH) .......................................... NUMBER EXAMINED: 11 0 NOT REMARKABLE: 10 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 10 3647 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 11 20 ------------------------------------------------------------------------ -=- -=- ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 11 0 NOT REMARKABLE: 11 0 TRACHEA (TR) ......................................... NUMBER EXAMINED: 11 0 NOT REMARKABLE: 11 0 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 11 0 NOT REMARKABLE: 4 0 --HEMORRHAGE --HYPERTROPHY --VACUOLATION 10 20 70 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 11 0 NOT REMARKABLE: 7 0 --ONE EXAMINED --B-PHEOCHROMOCYTOMA 10 40 PITUITARY (PI) ....................................... NUMBER EXAMINED: 11 9 NOT REMARKABLE: 1 0 --CYST --HEMORRHAGE --HYPERPLASIA --B-ADENOMA 01 37 10 99 3648 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 11 20 ------------------------------------------------------------------------ -=- -=- HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 11 0 NOT REMARKABLE: 10 0 --INFLAMMATION, CHRONIC 10 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 11 0 NOT REMARKABLE: 11 0 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 11 6 NOT REMARKABLE: 9 3 --EDEMA --EROSION --MINERALIZATION 11 13 10 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 11 1 NOT REMARKABLE: 10 0 --EROSION --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --B-LEIOMYOMA --M-CARCINOMA, SQUAMOUS CELL 10 10 01 10 01 DUODENUM (DU) ........................................ NUMBER EXAMINED: 11 0 NOT REMARKABLE: 11 0 3649 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 11 20 ------------------------------------------------------------------------ -=- -=- JEJUNUM (JE) ......................................... NUMBER EXAMINED: 11 0 NOT REMARKABLE: 11 0 ILEUM (IL) ........................................... NUMBER EXAMINED: 11 0 NOT REMARKABLE: 11 0 CECUM (CE) ........................................... NUMBER EXAMINED: 11 0 NOT REMARKABLE: 11 0 COLON (CO) ........................................... NUMBER EXAMINED: 11 0 NOT REMARKABLE: 11 0 RECTUM (RE) .......................................... NUMBER EXAMINED: 11 0 NOT REMARKABLE: 11 0 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 11 20 NOT REMARKABLE: 9 7 --CALCULUS --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, CHRONIC 0 13 20 10 3650 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 11 20 ------------------------------------------------------------------------ -=- -=- UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 OVARY (OV) ........................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 PROSTATE (PR) ........................................ NUMBER EXAMINED: 11 0 NOT REMARKABLE: 2 0 --EDEMA --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE 10 30 20 40 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 11 0 NOT REMARKABLE: 9 0 --INFLAMMATION, CHRONIC 10 3651 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 11 20 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 11 0 NOT REMARKABLE: 9 0 --INFLAMMATION, CHRONIC-ACTIVE 10 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 11 0 NOT REMARKABLE: 11 0 DEATH COMMENT (DC) ................................... NUMBER EXAMINED: 11 20 NOT REMARKABLE: 0 0 --SCHEDULED SACRIFICE 11 20 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 0 2 NOT REMARKABLE: 0 1 --HEMORRHAGE --INFILTRATE, MACROPHAGE, VACUOLATED 01 01 FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 3 2 NOT REMARKABLE: 0 0 --EROSION 20 3652 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 11 20 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 3 2 NOT REMARKABLE: 0 0 --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC ACTIVE 22 20 30 01 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 AORTA (AO) ........................................... NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 1 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 2 1 NOT REMARKABLE: 1 0 --DILATATION, SINUSOIDAL --FIBROSIS --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 11 01 11 10 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 2 1 NOT REMARKABLE: 1 0 --DILATATION, SINUSOIDAL 10 3653 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 11 20 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 2 1 NOT REMARKABLE: 1 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 01 HEMATO NEOPLASIA (HN) ................................ NUMBER EXAMINED: 11 2 NOT REMARKABLE: 10 0 --M-LYMPHOMA --M-SARCOMA, HISTIOCYTIC 10 02 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 5 0 NOT REMARKABLE: 0 0 --GALACTOCELE --MINERALIZATION --B-FIBROADENOMA 50 10 10 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 TAIL (TI) ............................................ NUMBER EXAMINED: 0 2 NOT REMARKABLE: 0 0 --CYST, EPIDERMAL INCLUSION --B-PAPILLOMA, SQUAMOUS CELL 01 01 3654 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 11 20 ------------------------------------------------------------------------ -=- -=- URETER (UE) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 1 0 NOT REMARKABLE: 0 0 --DILATATION, SINUSOIDAL 10 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 1 1 NOT REMARKABLE: 1 0 --INFILTRATE, MACROPHAGE, PIGMENTED 01 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 1 3655 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 11 20 ------------------------------------------------------------------------ -=- -=- AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 0 --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 01 01 01 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 2 0 NOT REMARKABLE: 0 0 --EDEMA --EXOSTOSES --FIBROSIS --INFLAMMATION, --INFLAMMATION, ACUTE, SYNOVIAL CHRONIC-ACTIVE, SYNOVIAL 20 20 10 10 10 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 3656 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 11 20 ------------------------------------------------------------------------ -=- -=- NASAL TURBINATE (NT) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 3657 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 13 15 ------------------------------------------------------------------------ -=- -=- ** TOP OF LIST ** LUNG (LU) ............................................ NUMBER EXAMINED: 13 15 NOT REMARKABLE: 8 10 --HEMORRHAGE --INFILTRATE, MACROPHAGE, ALVEOLAR --INFLAMMATION, GRANULOMATOUS --MINERALIZATION --MINERALIZATION, VASCULAR 10 11 10 01 23 KIDNEY (KD) .......................................... NUMBER EXAMINED: 13 15 NOT REMARKABLE: 0 0 --CAST, PROTEINACEOUS --CYST --DILATATION, PELVIC --INFLAMMATION, CHRONIC --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --NEPHROPATHY, CHRONIC PROGRESSIVE --PYELONEPHRITIS 57 01 01 01 7 10 10 44 01 LIVER (LI) ........................................... NUMBER EXAMINED: 13 15 NOT REMARKABLE: 7 3 --CYST, BILIARY --DEGENERATION, CYSTIC --FIBROSIS --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC 10 11 10 12 3658 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 13 15 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 13 15 NOT REMARKABLE: 7 3 --FOCUS, ALTERED HEPATOCELLULAR, --HYPERPLASIA, BILE DUCT --INFLAMMATION, CHRONIC --NECROSIS, HEPATOCELLULAR --TELANGIECTASIS --THROMBUS --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR EOSINOPHILIC 35 22 44 10 32 01 21 10 PANCREAS (PA) ........................................ NUMBER EXAMINED: 13 15 NOT REMARKABLE: 8 13 --ATROPHY, ACINAR --HEMORRHAGE --B-ADENOMA, ISLET CELL 21 20 31 THYROID (TY) ......................................... NUMBER EXAMINED: 13 15 NOT REMARKABLE: 7 8 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --HYPERPLASIA, C-CELL --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL 10 52 02 43 01 3659 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 13 15 ------------------------------------------------------------------------ -=- -=- PARATHYROID (PT) ..................................... NUMBER EXAMINED: 13 1 NOT REMARKABLE: 11 1 --HYPERPLASIA 20 TESTIS (TE) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 SKIN (SK) ............................................ NUMBER EXAMINED: 13 3 NOT REMARKABLE: 11 0 --CYST, EPIDERMAL INCLUSION --FIBROSIS --INFLAMMATION, CHRONIC-ACTIVE --B-KERATOACANTHOMA --B-PAPILLOMA, SQUAMOUS CELL --B-ADENOMA, SEBACEOUS GLAND 01 10 01 01 01 10 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 13 15 NOT REMARKABLE: 3 3 --FIBROSIS --GALACTOCELE --HEMORRHAGE --INFLAMMATION, ACUTE --MINERALIZATION --PIGMENT 10 9 10 40 10 32 10 3660 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 20 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 13 15 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 13 15 NOT REMARKABLE: 3 3 --B-ADENOMA --B-FIBROADENOMA --M-CARCINOMA 11 66 61 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 13 0 NOT REMARKABLE: 13 0 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 13 0 NOT REMARKABLE: 13 0 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 13 0 NOT REMARKABLE: 13 0 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 13 0 NOT REMARKABLE: 13 0 EYE (EY) ............................................. NUMBER EXAMINED: 13 0 NOT REMARKABLE: 11 0 --FIBROSIS, CORNEAL --INFLAMMATION, ACUTE, CORNEAL 10 20 3661 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 21 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 13 15 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** EYE (EY) ............................................. NUMBER EXAMINED: 13 0 NOT REMARKABLE: 11 0 --ULCERATION, CORNEAL --VASCULARIZATION, CORNEAL 10 10 BRAIN (BR) ........................................... NUMBER EXAMINED: 13 10 NOT REMARKABLE: 7 0 --COMPRESSION, VENTRAL --DILATATION, VENTRICULAR --INFLAMMATION, CHRONIC 5 10 42 10 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 13 0 NOT REMARKABLE: 13 0 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 13 1 NOT REMARKABLE: 13 0 --I-LEIOMYOSARCOMA 01 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 13 0 NOT REMARKABLE: 13 0 3662 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 22 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 13 15 ------------------------------------------------------------------------ -=- -=- HEART (HT) ........................................... NUMBER EXAMINED: 13 0 NOT REMARKABLE: 9 0 --DEGENERATION/NECROSIS --FIBROSIS --INFLAMMATION, CHRONIC --METAPLASIA, CARTILAGE 30 20 10 10 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 13 0 NOT REMARKABLE: 11 0 --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 10 20 SPLEEN (SP) .......................................... NUMBER EXAMINED: 13 1 NOT REMARKABLE: 12 0 --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED 11 THYMUS (TH) .......................................... NUMBER EXAMINED: 13 0 NOT REMARKABLE: 9 0 --CYST --M-THYMIC CARCINOMA 30 10 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 13 0 NOT REMARKABLE: 13 0 3663 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 23 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 13 15 ------------------------------------------------------------------------ -=- -=- TRACHEA (TR) ......................................... NUMBER EXAMINED: 13 0 NOT REMARKABLE: 13 0 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 13 4 NOT REMARKABLE: 1 0 --ANGIECTASIS --DEGENERATION, CYSTIC, --HEMORRHAGE --MINERALIZATION --PIGMENT --THROMBUS --VACUOLATION --B-ADENOMA CORTICAL 83 51 12 4 10 22 41 20 20 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 13 1 NOT REMARKABLE: 13 0 --NECROSIS, MEDULLARY --B-PHEOCHROMOCYTOMA 01 01 PITUITARY (PI) ....................................... NUMBER EXAMINED: 13 12 NOT REMARKABLE: 1 0 --CYST --HEMORRHAGE --PIGMENT 01 10 11 12 3664 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 24 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 13 15 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** PITUITARY (PI) ....................................... NUMBER EXAMINED: 13 12 NOT REMARKABLE: 1 0 --B-ADENOMA 12 12 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 13 0 NOT REMARKABLE: 8 0 --HYPERPLASIA, GLANDULAR --INFLAMMATION, CHRONIC 10 40 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 13 0 NOT REMARKABLE: 13 0 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 13 1 NOT REMARKABLE: 12 0 --EDEMA --EROSION 01 10 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 13 0 NOT REMARKABLE: 13 0 DUODENUM (DU) ........................................ NUMBER EXAMINED: 13 0 NOT REMARKABLE: 13 0 3665 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 25 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 13 15 ------------------------------------------------------------------------ -=- -=- JEJUNUM (JE) ......................................... NUMBER EXAMINED: 13 0 NOT REMARKABLE: 13 0 ILEUM (IL) ........................................... NUMBER EXAMINED: 13 0 NOT REMARKABLE: 13 0 CECUM (CE) ........................................... NUMBER EXAMINED: 13 0 NOT REMARKABLE: 13 0 COLON (CO) ........................................... NUMBER EXAMINED: 13 0 NOT REMARKABLE: 13 0 RECTUM (RE) .......................................... NUMBER EXAMINED: 13 1 NOT REMARKABLE: 8 0 --PARASITISM --M-LEIOMYOSARCOMA 50 01 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 13 15 NOT REMARKABLE: 13 15 3666 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 26 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 13 15 ------------------------------------------------------------------------ -=- -=- UTERUS (UT) .......................................... NUMBER EXAMINED: 13 2 NOT REMARKABLE: 9 0 --DILATATION --HYPERPLASIA, CYSTIC, ENDOMETRIAL --B-POLYP, ENDOMETRIAL, STROMAL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 32 01 10 10 OVARY (OV) ........................................... NUMBER EXAMINED: 13 5 NOT REMARKABLE: 9 3 --CYST --B-GRANULOSA/THECA CELL TUMOR 42 10 CERVIX (CV) .......................................... NUMBER EXAMINED: 13 1 NOT REMARKABLE: 12 0 --HYPERTROPHY, MUSCULAR --B-LEIOMYOMA 01 10 VAGINA (VA) .......................................... NUMBER EXAMINED: 13 0 NOT REMARKABLE: 13 0 PROSTATE (PR) ........................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 3667 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 13 15 ------------------------------------------------------------------------ -=- -=- SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 DEATH COMMENT (DC) ................................... NUMBER EXAMINED: 13 15 NOT REMARKABLE: 0 0 --SCHEDULED SACRIFICE 13 15 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 2 2 NOT REMARKABLE: 0 1 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC 10 11 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 4 3 NOT REMARKABLE: 4 1 --HEMORRHAGE --INFILTRATE, MACROPHAGE, VACUOLATED 02 01 3668 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 28 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 13 15 ------------------------------------------------------------------------ -=- -=- FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 2 3 NOT REMARKABLE: 0 0 --CYST --EROSION --EXOSTOSES --FIBROSIS --INFLAMMATION, --INFLAMMATION, --PIGMENT ACUTE CHRONIC ACTIVE 01 22 01 21 21 01 01 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 1 0 NOT REMARKABLE: 0 0 --HYPERPLASIA, LYMPHOCYTIC 10 AORTA (AO) ........................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 1 2 NOT REMARKABLE: 1 2 3669 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 29 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 13 15 ------------------------------------------------------------------------ -=- -=- HEMATO NEOPLASIA (HN) ................................ NUMBER EXAMINED: 13 0 NOT REMARKABLE: 12 0 --M-SARCOMA, HISTIOCYTIC 10 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 1 0 NOT REMARKABLE: 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 10 TAIL (TI) ............................................ NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 0 --EROSION --INFLAMMATION, ACUTE --M-FIBROSARCOMA 01 01 01 URETER (UE) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 3670 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 30 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 13 15 ------------------------------------------------------------------------ -=- -=- CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 2 0 NOT REMARKABLE: 0 0 --HEMORRHAGE 20 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 1 0 NOT REMARKABLE: 1 0 3671 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 31 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 13 15 ------------------------------------------------------------------------ -=- -=- ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 1 0 NOT REMARKABLE: 0 0 --M-LIPOSARCOMA 10 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 0 --EXOSTOSES --FIBROSIS --INFLAMMATION, CHRONIC, SYNOVIAL 01 01 01 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 NASAL TURBINATE (NT) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 3672 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ------------------------------------------------------------------------ -=- -=- ** TOP OF LIST ** LUNG (LU) ............................................ NUMBER EXAMINED: 39 30 NOT REMARKABLE: 31 20 --FIBROSIS, PLEURAL/SUBPLEURAL --INFLAMMATION, CHRONIC --INFILTRATE, MACROPHAGE, ALVEOLAR --INFLAMMATION, GRANULOMATOUS --MINERALIZATION --MINERALIZATION, VASCULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --N-TRANSITIONAL CELL CARCINOMA 11 23 31 01 01 35 11 10 KIDNEY (KD) .......................................... NUMBER EXAMINED: 39 30 NOT REMARKABLE: 2 3 --CAST, PROTEINACEOUS --CYST --DILATATION, PELVIC --HYPERPLASIA, TRANSITIONAL CELL --INFARCT --INFLAMMATION, CHRONIC --MINERALIZATION --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --NEPHROPATHY, CHRONIC PROGRESSIVE --PYELONEPHRITIS --THROMBUS --I-TRANSITIONAL CELL CARCINOMA 12 5 21 85 01 21 02 11 32 12 23 19 60 40 10 3673 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ------------------------------------------------------------------------ -=- -=- LIVER (LI) ........................................... NUMBER EXAMINED: 39 30 NOT REMARKABLE: 14 15 --CYST --DEGENERATION, CYSTIC --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERPLASIA, BILE DUCT --INFILTRATE, MAST CELLS --INFLAMMATION, CHRONIC --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) --NECROSIS, HEPATOCELLULAR --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 20 34 33 21 21 33 01 10 3 11 13 14 3 42 12 PANCREAS (PA) ........................................ NUMBER EXAMINED: 39 30 NOT REMARKABLE: 28 19 --ATROPHY, ACINAR --HEMORRHAGE --HYPERTROPHY, VASCULAR --INFLAMMATION, VASCULAR --PIGMENT --THROMBUS --B-ADENOMA, ISLET CELL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 42 01 11 01 64 11 25 11 3674 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ------------------------------------------------------------------------ -=- -=- THYROID (TY) ......................................... NUMBER EXAMINED: 39 30 NOT REMARKABLE: 29 19 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --HYPERPLASIA, C-CELL --HYPERPLASIA, FOLLICULAR CELL --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL 54 44 01 11 21 01 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 38 4 NOT REMARKABLE: 32 2 --HYPERPLASIA 62 TESTIS (TE) .......................................... NUMBER EXAMINED: 39 25 NOT REMARKABLE: 33 21 --ATROPHY/DEGENERATION --INFLAMMATION, CHRONIC --MINERALIZATION --MINERALIZATION, VASCULAR --SPERMATOCELE 33 21 30 01 10 SKIN (SK) ............................................ NUMBER EXAMINED: 39 11 NOT REMARKABLE: 32 2 --ATROPHY, ADNEXAL 01 3675 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** SKIN (SK) ............................................ NUMBER EXAMINED: 39 11 NOT REMARKABLE: 32 2 --CYST, EPIDERMAL INCLUSION --EDEMA --EROSION --HEMORRHAGE --INFLAMMATION, ACUTE --B-BASAL CELL TUMOR --B-FIBROMA --B-KERATOACANTHOMA --M-CARCINOMA, SQUAMOUS CELL --M-FIBROSARCOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 11 11 03 11 15 10 30 02 01 01 11 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 39 0 NOT REMARKABLE: 38 0 --HEMORRHAGE --INFLAMMATION, CHRONIC 10 10 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 39 0 NOT REMARKABLE: 39 0 3676 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ------------------------------------------------------------------------ -=- -=- MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 39 0 NOT REMARKABLE: 39 0 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 39 0 NOT REMARKABLE: 39 0 EYE (EY) ............................................. NUMBER EXAMINED: 39 1 NOT REMARKABLE: 35 1 --INFLAMMATION, ACUTE, CORNEAL --INFLAMMATION, ACUTE, CILIARY --ULCERATION, CORNEAL --VASCULARIZATION, CORNEAL 40 20 10 20 BRAIN (BR) ........................................... NUMBER EXAMINED: 39 16 NOT REMARKABLE: 20 2 --COMPRESSION, VENTRAL --DILATATION, VENTRICULAR --HEMORRHAGE 19 14 14 7 20 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 39 0 NOT REMARKABLE: 38 0 --HEMORRHAGE 10 3677 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ------------------------------------------------------------------------ -=- -=- MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 39 1 NOT REMARKABLE: 39 0 --I-SQUAMOUS CELL CARCINOMA 01 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 39 0 NOT REMARKABLE: 39 0 HEART (HT) ........................................... NUMBER EXAMINED: 39 3 NOT REMARKABLE: 17 1 --DEGENERATION/NECROSIS --FIBROSIS --MINERALIZATION --THROMBUS, ATRIAL 21 2 14 1 10 20 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 38 4 NOT REMARKABLE: 34 4 --DILATATION, SINUSOIDAL --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 10 20 20 SPLEEN (SP) .......................................... NUMBER EXAMINED: 39 4 NOT REMARKABLE: 36 1 --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED 22 3678 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** SPLEEN (SP) .......................................... NUMBER EXAMINED: 39 4 NOT REMARKABLE: 36 1 --THROMBUS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 01 10 THYMUS (TH) .......................................... NUMBER EXAMINED: 38 1 NOT REMARKABLE: 34 0 --CYST --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 30 20 01 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 39 0 NOT REMARKABLE: 39 0 TRACHEA (TR) ......................................... NUMBER EXAMINED: 39 0 NOT REMARKABLE: 39 0 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 39 6 NOT REMARKABLE: 17 1 --ANGIECTASIS --DEGENERATION, CYSTIC, CORTICAL --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE 10 51 01 62 3679 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 39 6 NOT REMARKABLE: 17 1 --HYPERTROPHY --ONE EXAMINED --THROMBUS --VACUOLATION 21 20 10 13 3 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 39 3 NOT REMARKABLE: 33 0 --ONE EXAMINED --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --B-PHEOCHROMOCYTOMA 20 01 01 43 PITUITARY (PI) ....................................... NUMBER EXAMINED: 39 17 NOT REMARKABLE: 8 0 --CYST --HEMORRHAGE --INFLAMMATION, --PIGMENT --B-ADENOMA CHRONIC 12 14 14 10 02 30 17 3680 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ------------------------------------------------------------------------ -=- -=- HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 39 0 NOT REMARKABLE: 35 0 --INFLAMMATION, CHRONIC 40 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 39 0 NOT REMARKABLE: 39 0 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 39 11 NOT REMARKABLE: 34 6 --EDEMA --EROSION --INFLAMMATION, ACUTE --MINERALIZATION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 20 33 10 11 01 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 39 3 NOT REMARKABLE: 28 1 --ACANTHOSIS --EDEMA --EROSION --FIBROSIS --HEMORRHAGE --HYPERKERATOSIS, NONGLANDULAR --INFLAMMATION, ACUTE MUCOSA 10 92 21 10 11 10 52 3681 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 39 3 NOT REMARKABLE: 28 1 --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION 10 21 DUODENUM (DU) ........................................ NUMBER EXAMINED: 38 0 NOT REMARKABLE: 37 0 --HEMORRHAGE --INFLAMMATION, ACUTE --ULCERATION 10 10 10 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 34 0 NOT REMARKABLE: 33 0 --HEMORRHAGE --INFLAMMATION, ACUTE --ULCERATION 10 10 10 ILEUM (IL) ........................................... NUMBER EXAMINED: 32 0 NOT REMARKABLE: 31 0 --EROSION --INFLAMMATION, ACUTE 10 10 3682 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ------------------------------------------------------------------------ -=- -=- CECUM (CE) ........................................... NUMBER EXAMINED: 36 1 NOT REMARKABLE: 35 1 --EDEMA --INFLAMMATION, ACUTE --ULCERATION 10 10 10 COLON (CO) ........................................... NUMBER EXAMINED: 38 0 NOT REMARKABLE: 36 0 --EDEMA --PARASITISM 10 10 RECTUM (RE) .......................................... NUMBER EXAMINED: 38 0 NOT REMARKABLE: 37 0 --PARASITISM 10 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 39 2 NOT REMARKABLE: 33 1 --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 10 41 11 20 10 10 3683 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ------------------------------------------------------------------------ -=- -=- UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 OVARY (OV) ........................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 PROSTATE (PR) ........................................ NUMBER EXAMINED: 39 10 NOT REMARKABLE: 16 1 --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --I-TRANSITIONAL CELL CARCINOMA 01 61 16 7 01 10 3684 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ------------------------------------------------------------------------ -=- -=- SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 39 5 NOT REMARKABLE: 31 1 --EDEMA --FIBROSIS --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --PIGMENT --SECRETION, INCREASED --M-TRANSITIONAL CELL CARCINOMA 11 10 20 20 10 10 23 10 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 39 2 NOT REMARKABLE: 37 0 --GRANULOMA, SPERM --HYPOSPERMIA --INFLAMMATION, CHRONIC 11 11 01 DEATH COMMENT (DC) ................................... NUMBER EXAMINED: 39 30 NOT REMARKABLE: 0 0 --ATRIAL THROMBUS --AUDITORY SEBACEOUS GLAND CARCINOMA --CHRONIC PROGRESSIVE NEPHROPATHY --CYSTITIS --ENTERITIS --FIBROMA 10 01 32 20 10 10 3685 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** DEATH COMMENT (DC) ................................... NUMBER EXAMINED: 39 30 NOT REMARKABLE: 0 0 --FIBROSARCOMA --HEPATOCELLULAR ADENOMA --HEPATOCELLULAR NECROSIS --HISTIOCYTIC SARCOMA --MALIGNANT LYMPHOMA --MAMMARY FIBROADENOMA --OSTEOGENIC SARCOMA --PHEOCHROMOCYTOMA --PITUITARY ADENOMA --PYELONEPHRITIS --SQUAMOUS CELL CARCINOMA --TRANSITIONAL CELL CARCINOMA --UNDETERMINED 01 21 10 12 01 10 01 01 18 14 10 01 10 65 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 4 4 NOT REMARKABLE: 2 2 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC 21 02 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 3686 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ------------------------------------------------------------------------ -=- -=- FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 8 4 NOT REMARKABLE: 0 1 --CYST, KERATIN --EDEMA --EROSION --EXOSTOSES --FIBROSIS --HEMORRHAGE --INFLAMMATION, --INFLAMMATION, ACUTE CHRONIC ACTIVE 10 31 62 10 32 10 73 10 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 AORTA (AO) ........................................... NUMBER EXAMINED: 1 1 NOT REMARKABLE: 0 0 --MINERALIZATION 11 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 0 2 NOT REMARKABLE: 0 0 --DILATATION, SINUSOIDAL --HEMORRHAGE 01 02 3687 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ------------------------------------------------------------------------ -=- -=- LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 3 6 NOT REMARKABLE: 0 1 --DILATATION, SINUSOIDAL --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 24 10 01 HEMATO NEOPLASIA (HN) ................................ NUMBER EXAMINED: 39 3 NOT REMARKABLE: 37 0 --M-LYMPHOMA --M-SARCOMA, HISTIOCYTIC 11 12 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 6 5 NOT REMARKABLE: 1 3 --GALACTOCELE --INFLAMMATION, CHRONIC --B-FIBROADENOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 41 10 10 01 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 3688 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ------------------------------------------------------------------------ -=- -=- TAIL (TI) ............................................ NUMBER EXAMINED: 2 1 NOT REMARKABLE: 0 0 --HEMORRHAGE --INFLAMMATION, ACUTE --B-FIBROMA 10 01 10 URETER (UE) .......................................... NUMBER EXAMINED: 2 0 NOT REMARKABLE: 0 0 --DILATATION 20 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 1 1 NOT REMARKABLE: 0 0 --HEMORRHAGE --M-SARCOMA, OSTEOGENIC 10 01 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 1 1 NOT REMARKABLE: 0 1 --DILATATION, SINUSOIDAL --HEMORRHAGE 10 10 3689 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ------------------------------------------------------------------------ -=- -=- LN, OTHER (LN) ....................................... NUMBER EXAMINED: 1 1 NOT REMARKABLE: 1 0 --INFILTRATE, MACROPHAGE, PIGMENTED 01 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 0 --DILATATION 01 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 0 --M-CARCINOMA 01 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 0 --DILATATION, SINUSOIDAL --INFILTRATE, MACROPHAGE, PIGMENTED 01 01 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 0 --FAT NECROSIS 01 3690 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ------------------------------------------------------------------------ -=- -=- JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 NASAL TURBINATE (NT) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 3691 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 20 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 37 35 ------------------------------------------------------------------------ -=- -=- ** TOP OF LIST ** LUNG (LU) ............................................ NUMBER EXAMINED: 37 35 NOT REMARKABLE: 31 24 --HEMORRHAGE --HYPERPLASIA, TYPE II PNEUMOCYTE --INFLAMMATION, CHRONIC --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, MACROPHAGE, PIGMENTED --MINERALIZATION, VASCULAR 01 11 21 52 01 16 KIDNEY (KD) .......................................... NUMBER EXAMINED: 37 35 NOT REMARKABLE: 2 4 --CAST, PROTEINACEOUS --CYST --DILATATION, PELVIC --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFARCT --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --NEPHROPATHY, CHRONIC PROGRESSIVE --PYELONEPHRITIS 14 10 10 11 10 10 10 27 30 10 73 33 LIVER (LI) ........................................... NUMBER EXAMINED: 37 35 NOT REMARKABLE: 19 17 --CYST, BILIARY 10 3692 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 21 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 37 35 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 37 35 NOT REMARKABLE: 19 17 --DEGENERATION, CYSTIC --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERPLASIA, BILE DUCT --INFLAMMATION, CHRONIC --NECROSIS, HEPATOCELLULAR --PIGMENT --TELANGIECTASIS --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 01 01 02 11 12 34 42 12 10 20 12 7 11 01 PANCREAS (PA) ........................................ NUMBER EXAMINED: 37 35 NOT REMARKABLE: 36 30 --ATROPHY, ACINAR --HEMORRHAGE --B-ADENOMA, ISLET CELL 05 10 10 THYROID (TY) ......................................... NUMBER EXAMINED: 37 35 NOT REMARKABLE: 22 26 --CYST, ULTIMOBRANCHIAL 94 3693 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 22 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 37 35 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** THYROID (TY) ......................................... NUMBER EXAMINED: 37 35 NOT REMARKABLE: 22 26 --FIBROSIS --HEMORRHAGE --HYPERPLASIA, C-CELL --HYPERPLASIA, FOLLICULAR CELL --INFLAMMATION, CHRONIC --MINERALIZATION --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL 10 01 12 01 10 10 33 10 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 35 0 NOT REMARKABLE: 34 0 --FIBROSIS 10 TESTIS (TE) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 SKIN (SK) ............................................ NUMBER EXAMINED: 37 2 NOT REMARKABLE: 34 0 --EROSION --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE 10 01 10 10 3694 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 23 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 37 35 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** SKIN (SK) ............................................ NUMBER EXAMINED: 37 2 NOT REMARKABLE: 34 0 --PIGMENT --B-BASAL CELL --B-FIBROMA --B-PAPILLOMA, --M-CARCINOMA, TUMOR SQUAMOUS SQUAMOUS CELL CELL 01 01 10 10 01 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 37 35 NOT REMARKABLE: 2 5 --GALACTOCELE --HEMORRHAGE --HYPERPLASIA, GLANDULAR --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --MINERALIZATION --NECROSIS --PIGMENT --B-ADENOMA --B-FIBROADENOMA --B-FIBROMA --M-CARCINOMA 35 27 24 13 21 01 58 20 32 41 14 15 10 38 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 37 0 NOT REMARKABLE: 37 0 3695 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 24 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 37 35 ------------------------------------------------------------------------ -=- -=- MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 37 0 NOT REMARKABLE: 36 0 --HYPERCELLULARITY, MARROW 10 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 37 0 NOT REMARKABLE: 36 0 --HYPERCELLULARITY, MARROW 10 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 37 0 NOT REMARKABLE: 37 0 EYE (EY) ............................................. NUMBER EXAMINED: 37 0 NOT REMARKABLE: 36 0 --INFLAMMATION, ACUTE, CILIARY 10 BRAIN (BR) ........................................... NUMBER EXAMINED: 37 29 NOT REMARKABLE: 6 0 --COMPRESSION, VENTRAL --DILATATION, VENTRICULAR --HEMORRHAGE --PIGMENT 31 29 15 21 03 01 3696 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 25 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 37 35 ------------------------------------------------------------------------ -=- -=- SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 37 1 NOT REMARKABLE: 37 1 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 37 1 NOT REMARKABLE: 36 1 --MINERALIZATION 10 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 37 1 NOT REMARKABLE: 37 1 HEART (HT) ........................................... NUMBER EXAMINED: 37 2 NOT REMARKABLE: 27 1 --DEGENERATION/NECROSIS --FIBROSIS --INFLAMMATION, CHRONIC 90 51 11 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 37 2 NOT REMARKABLE: 34 2 --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 20 20 3697 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 26 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 37 35 ------------------------------------------------------------------------ -=- -=- SPLEEN (SP) .......................................... NUMBER EXAMINED: 37 4 NOT REMARKABLE: 34 1 --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED --PIGMENT, INCREASED 23 10 THYMUS (TH) .......................................... NUMBER EXAMINED: 37 2 NOT REMARKABLE: 29 1 --CYST --M-FIBROSARCOMA 80 01 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 37 0 NOT REMARKABLE: 37 0 TRACHEA (TR) ......................................... NUMBER EXAMINED: 37 0 NOT REMARKABLE: 37 0 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 37 8 NOT REMARKABLE: 8 0 --ANGIECTASIS --DEGENERATION, CYSTIC, CORTICAL --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERTROPHY 22 5 11 8 11 26 8 10 3698 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 37 35 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 37 8 NOT REMARKABLE: 8 0 --PIGMENT --THROMBUS --VACUOLATION 52 73 21 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 37 3 NOT REMARKABLE: 35 2 --HYPERPLASIA, MEDULLARY --B-PHEOCHROMOCYTOMA 10 11 PITUITARY (PI) ....................................... NUMBER EXAMINED: 36 31 NOT REMARKABLE: 2 0 --CYST --HEMORRHAGE --PIGMENT --B-ADENOMA --M-CARCINOMA 45 27 20 63 34 30 01 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 37 0 NOT REMARKABLE: 33 0 --INFLAMMATION, CHRONIC 40 3699 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 28 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 37 35 ------------------------------------------------------------------------ -=- -=- SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 37 0 NOT REMARKABLE: 37 0 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 37 2 NOT REMARKABLE: 36 2 --EROSION --INFLAMMATION, ACUTE 10 10 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 37 3 NOT REMARKABLE: 31 0 --ACANTHOSIS --EDEMA --EROSION --HEMORRHAGE --HYPERKERATOSIS, NONGLANDULAR --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION MUCOSA 01 52 32 10 01 31 11 20 DUODENUM (DU) ........................................ NUMBER EXAMINED: 35 0 NOT REMARKABLE: 35 0 3700 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 29 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 37 35 ------------------------------------------------------------------------ -=- -=- JEJUNUM (JE) ......................................... NUMBER EXAMINED: 35 0 NOT REMARKABLE: 35 0 ILEUM (IL) ........................................... NUMBER EXAMINED: 32 0 NOT REMARKABLE: 32 0 CECUM (CE) ........................................... NUMBER EXAMINED: 37 3 NOT REMARKABLE: 37 1 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 02 COLON (CO) ........................................... NUMBER EXAMINED: 37 0 NOT REMARKABLE: 37 0 RECTUM (RE) .......................................... NUMBER EXAMINED: 37 0 NOT REMARKABLE: 37 0 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 37 0 NOT REMARKABLE: 35 0 --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, CHRONIC 10 20 3701 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 30 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 37 35 ------------------------------------------------------------------------ -=- -=- UTERUS (UT) .......................................... NUMBER EXAMINED: 37 7 NOT REMARKABLE: 31 0 --DILATATION --HYPERPLASIA, CYSTIC, ENDOMETRIAL --SCLEROSIS --B-POLYP, ENDOMETRIAL, STROMAL 43 24 01 02 OVARY (OV) ........................................... NUMBER EXAMINED: 37 7 NOT REMARKABLE: 32 1 --CYST --INFLAMMATION, CHRONIC 46 10 CERVIX (CV) .......................................... NUMBER EXAMINED: 37 1 NOT REMARKABLE: 37 0 --DILATATION --INFLAMMATION, ACUTE --M-SARCOMA, ENDOMETRIAL STROMAL 01 01 01 VAGINA (VA) .......................................... NUMBER EXAMINED: 37 8 NOT REMARKABLE: 36 7 --INFLAMMATION, ACUTE --N-SARCOMA, ENDOMETRIAL STROMAL 11 01 3702 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 31 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 37 35 ------------------------------------------------------------------------ -=- -=- PROSTATE (PR) ........................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 DEATH COMMENT (DC) ................................... NUMBER EXAMINED: 37 35 NOT REMARKABLE: 0 0 --ENDOMETRIAL STROMAL SARCOMA --FRACTURED CALVARIUM --HISTIOCYTIC SARCOMA --MALIGNANT LYMPHOMA --MAMMARY CARCINOMA --MAMMARY FIBROADENOMA --ORAL EROSION --OSTEOMA --PITUITARY ADENOMA --PITUITARY CARCINOMA --PYELONEPHRITIS --UNDETERMINED 01 10 01 01 03 32 10 10 29 25 01 10 11 3703 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 32 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 37 35 ------------------------------------------------------------------------ -=- -=- LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 8 6 NOT REMARKABLE: 4 4 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED 22 20 10 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 11 11 NOT REMARKABLE: 7 9 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, VACUOLATED 02 40 01 FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 0 3 NOT REMARKABLE: 0 0 --EDEMA --EROSION --EXOSTOSES --FIBROSIS --INFLAMMATION, --INFLAMMATION, ACUTE CHRONIC ACTIVE 01 01 01 02 01 01 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 2 1 NOT REMARKABLE: 1 1 --N-MAMMARY CARCINOMA 10 3704 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 33 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 37 35 ------------------------------------------------------------------------ -=- -=- AORTA (AO) ........................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 0 2 NOT REMARKABLE: 0 2 HEMATO NEOPLASIA (HN) ................................ NUMBER EXAMINED: 37 2 NOT REMARKABLE: 37 0 --M-LYMPHOMA --M-SARCOMA, HISTIOCYTIC 01 01 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 3705 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 34 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 37 35 ------------------------------------------------------------------------ -=- -=- TAIL (TI) ............................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 URETER (UE) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 1 0 NOT REMARKABLE: 0 0 --EDEMA --EROSION --HEMORRHAGE --INFLAMMATION, ACUTE 10 10 10 10 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 1 0 NOT REMARKABLE: 0 0 --CYST, KERATIN --FRACTURE --HEMORRHAGE --INFLAMMATION, ACUTE 10 10 10 10 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 2 1 NOT REMARKABLE: 0 0 --HEMORRHAGE 01 3706 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 35 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 37 35 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 2 1 NOT REMARKABLE: 0 0 --INFILTRATE, MACROPHAGE, PIGMENTED 20 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 0 --B-LIPOMA 01 3707 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 36 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 37 35 ------------------------------------------------------------------------ -=- -=- JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 NASAL TURBINATE (NT) ................................. NUMBER EXAMINED: 1 0 NOT REMARKABLE: 0 0 --B-OSTEOMA 10 3708 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- ** TOP OF LIST ** LUNG (LU) ............................................ NUMBER EXAMINED: 60 60 NOT REMARKABLE: 52 44 --FIBROSIS, PLEURAL/SUBPLEURAL --HEMORRHAGE --INFLAMMATION, CHRONIC --INFILTRATE, MACROPHAGE, ALVEOLAR --INFLAMMATION, GRANULOMATOUS --MINERALIZATION --MINERALIZATION, VASCULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --N-TRANSITIONAL CELL CARCINOMA 11 01 23 34 01 01 38 12 10 KIDNEY (KD) .......................................... NUMBER EXAMINED: 60 60 NOT REMARKABLE: 7 4 --CAST, PROTEINACEOUS --CYST --DILATATION, PELVIC --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFARCT --INFLAMMATION, CHRONIC --MINERALIZATION --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --NEPHROPATHY, CHRONIC PROGRESSIVE --PYELONEPHRITIS 18 17 33 98 10 11 23 02 11 77 12 33 35 70 3709 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** KIDNEY (KD) .......................................... NUMBER EXAMINED: 60 60 NOT REMARKABLE: 7 4 --THROMBUS --M-CARCINOMA, TUBULAR CELL --I-TRANSITIONAL CELL CARCINOMA 40 10 10 LIVER (LI) ........................................... NUMBER EXAMINED: 60 60 NOT REMARKABLE: 22 25 --CYST --DEGENERATION, CYSTIC --FIBROSIS, CAPSULAR --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERPLASIA, BILE DUCT --INFILTRATE, MAST CELLS --INFLAMMATION, CHRONIC --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) --NECROSIS, HEPATOCELLULAR --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 20 59 01 01 49 21 52 78 01 17 15 21 23 14 10 54 14 3710 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- PANCREAS (PA) ........................................ NUMBER EXAMINED: 60 60 NOT REMARKABLE: 41 35 --ATROPHY, ACINAR --FIBROSIS --HEMORRHAGE --HYPERPLASIA, ISLET CELLS --HYPERTROPHY, VASCULAR --INFLAMMATION, VASCULAR --PIGMENT --THROMBUS --B-ADENOMA, ACINAR CELL --B-ADENOMA, ISLET CELL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 97 01 12 10 31 11 79 11 01 4 11 12 THYROID (TY) ......................................... NUMBER EXAMINED: 60 60 NOT REMARKABLE: 42 38 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --FIBROSIS --HYPERPLASIA, C-CELL --HYPERPLASIA, FOLLICULAR CELL --INFLAMMATION, CHRONIC --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL 10 9 69 11 11 12 11 23 12 3711 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- PARATHYROID (PT) ..................................... NUMBER EXAMINED: 59 4 NOT REMARKABLE: 52 2 --HYPERPLASIA 72 TESTIS (TE) .......................................... NUMBER EXAMINED: 60 55 NOT REMARKABLE: 49 41 --ATROPHY/DEGENERATION --CYST --INFLAMMATION, CHRONIC --MINERALIZATION --MINERALIZATION, VASCULAR --SPERMATOCELE --B-INTERSTITIAL CELL TUMOR 78 01 44 34 02 10 03 SKIN (SK) ............................................ NUMBER EXAMINED: 60 15 NOT REMARKABLE: 49 2 --ATROPHY, ADNEXAL --CYST, EPIDERMAL INCLUSION --EDEMA --EROSION --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --B-BASAL CELL TUMOR 02 22 11 05 01 21 36 01 10 3712 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** SKIN (SK) ............................................ NUMBER EXAMINED: 60 15 NOT REMARKABLE: 49 2 --B-ADENOMA, BASAL CELL --B-FIBROMA --B-KERATOACANTHOMA --B-PAPILLOMA, SQUAMOUS CELL --M-CARCINOMA, SQUAMOUS CELL --M-FIBROSARCOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 11 32 02 10 11 01 11 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 60 0 NOT REMARKABLE: 59 0 --HEMORRHAGE --INFLAMMATION, CHRONIC 10 10 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 60 0 NOT REMARKABLE: 60 0 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 60 0 NOT REMARKABLE: 60 0 3713 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 60 0 NOT REMARKABLE: 60 0 EYE (EY) ............................................. NUMBER EXAMINED: 60 2 NOT REMARKABLE: 56 1 --CATARACT --INFLAMMATION, ACUTE, CORNEAL --INFLAMMATION, ACUTE, CILIARY --ULCERATION, CORNEAL --VASCULARIZATION, CORNEAL 01 40 20 10 20 BRAIN (BR) ........................................... NUMBER EXAMINED: 60 18 NOT REMARKABLE: 34 2 --COMPRESSION, VENTRAL --DILATATION, VENTRICULAR --HEMORRHAGE --INFLAMMATION, CHRONIC --MINERALIZATION 23 16 17 7 20 10 20 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 60 0 NOT REMARKABLE: 59 0 --HEMORRHAGE 10 3714 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 60 1 NOT REMARKABLE: 60 0 --I-SQUAMOUS CELL CARCINOMA 01 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 59 0 NOT REMARKABLE: 59 0 HEART (HT) ........................................... NUMBER EXAMINED: 60 3 NOT REMARKABLE: 29 1 --DEGENERATION/NECROSIS --FIBROSIS --MINERALIZATION --THROMBUS, ATRIAL 30 2 22 1 10 20 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 59 6 NOT REMARKABLE: 55 5 --DILATATION, SINUSOIDAL --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --N-HEMANGIOSARCOMA 10 21 20 01 3715 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- SPLEEN (SP) .......................................... NUMBER EXAMINED: 60 8 NOT REMARKABLE: 57 3 --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED --THROMBUS --B-LEIOMYOMA --M-HEMANGIOSARCOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 22 01 01 01 10 THYMUS (TH) .......................................... NUMBER EXAMINED: 59 1 NOT REMARKABLE: 54 0 --CYST --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 30 20 11 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 60 0 NOT REMARKABLE: 60 0 TRACHEA (TR) ......................................... NUMBER EXAMINED: 60 0 NOT REMARKABLE: 60 0 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 60 6 NOT REMARKABLE: 31 1 --ANGIECTASIS 10 3716 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 60 6 NOT REMARKABLE: 31 1 --DEGENERATION, CYSTIC, CORTICAL --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERTROPHY --ONE EXAMINED --THROMBUS --VACUOLATION 51 01 72 41 20 10 20 3 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 60 3 NOT REMARKABLE: 50 0 --ONE EXAMINED --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --B-PHEOCHROMOCYTOMA 30 01 01 83 PITUITARY (PI) ....................................... NUMBER EXAMINED: 59 27 NOT REMARKABLE: 17 0 --CYST --HEMORRHAGE --HYPERPLASIA --INFLAMMATION, --PIGMENT --B-ADENOMA CHRONIC 13 17 22 10 10 02 40 27 3717 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 60 0 NOT REMARKABLE: 54 0 --INFLAMMATION, CHRONIC --ONE EXAMINED 50 10 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 60 0 NOT REMARKABLE: 60 0 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 60 17 NOT REMARKABLE: 52 9 --EDEMA --EROSION --INFLAMMATION, ACUTE --MINERALIZATION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 41 46 10 21 01 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 60 4 NOT REMARKABLE: 48 1 --ACANTHOSIS --EDEMA --EROSION --FIBROSIS --HEMORRHAGE --HYPERKERATOSIS, NONGLANDULAR MUCOSA 10 92 31 10 11 10 3718 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 60 4 NOT REMARKABLE: 48 1 --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --B-LEIOMYOMA --M-CARCINOMA, SQUAMOUS CELL 62 11 21 10 01 DUODENUM (DU) ........................................ NUMBER EXAMINED: 59 0 NOT REMARKABLE: 58 0 --HEMORRHAGE --INFLAMMATION, ACUTE --ULCERATION 10 10 10 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 55 0 NOT REMARKABLE: 54 0 --HEMORRHAGE --INFLAMMATION, ACUTE --ULCERATION 10 10 10 ILEUM (IL) ........................................... NUMBER EXAMINED: 53 0 NOT REMARKABLE: 52 0 --EROSION --INFLAMMATION, ACUTE 10 10 3719 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- CECUM (CE) ........................................... NUMBER EXAMINED: 57 1 NOT REMARKABLE: 56 1 --EDEMA --INFLAMMATION, ACUTE --ULCERATION 10 10 10 COLON (CO) ........................................... NUMBER EXAMINED: 59 0 NOT REMARKABLE: 57 0 --EDEMA --PARASITISM 10 10 RECTUM (RE) .......................................... NUMBER EXAMINED: 59 0 NOT REMARKABLE: 57 0 --PARASITISM 20 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 60 22 NOT REMARKABLE: 52 8 --CALCULUS --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0 13 10 61 21 20 10 10 3720 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 OVARY (OV) ........................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 PROSTATE (PR) ........................................ NUMBER EXAMINED: 60 10 NOT REMARKABLE: 26 1 --EDEMA --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --I-TRANSITIONAL CELL CARCINOMA 10 01 10 1 20 21 7 01 10 3721 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 60 5 NOT REMARKABLE: 50 1 --EDEMA --FIBROSIS --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --PIGMENT --SECRETION, INCREASED --M-TRANSITIONAL CELL CARCINOMA 11 10 20 30 20 10 23 10 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 60 2 NOT REMARKABLE: 58 0 --GRANULOMA, SPERM --HYPOSPERMIA --INFLAMMATION, CHRONIC 11 11 01 DEATH COMMENT (DC) ................................... NUMBER EXAMINED: 60 60 NOT REMARKABLE: 0 0 --ATRIAL THROMBUS --AUDITORY SEBACEOUS GLAND CARCINOMA --CHRONIC PROGRESSIVE NEPHROPATHY --CYSTITIS --ENTERITIS --FIBROMA 10 01 32 20 10 10 3722 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** DEATH COMMENT (DC) ................................... NUMBER EXAMINED: 60 60 NOT REMARKABLE: 0 0 --FIBROSARCOMA --HEPATOCELLULAR ADENOMA --HEPATOCELLULAR NECROSIS --HISTIOCYTIC SARCOMA --MALIGNANT LYMPHOMA --MAMMARY FIBROADENOMA --OSTEOGENIC SARCOMA --PHEOCHROMOCYTOMA --PITUITARY ADENOMA --PYELONEPHRITIS --SCHEDULED SACRIFICE --SQUAMOUS CELL CARCINOMA --TRANSITIONAL CELL CARCINOMA --UNDETERMINED 01 21 10 12 01 10 01 01 18 14 10 21 30 01 10 65 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 4 4 NOT REMARKABLE: 2 2 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC 21 02 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 0 2 NOT REMARKABLE: 0 1 --HEMORRHAGE 01 3723 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 0 2 NOT REMARKABLE: 0 1 --INFILTRATE, MACROPHAGE, VACUOLATED 01 FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 11 6 NOT REMARKABLE: 0 1 --CYST, KERATIN --EDEMA --EROSION --EXOSTOSES --FIBROSIS --HEMORRHAGE --INFLAMMATION, --INFLAMMATION, ACUTE CHRONIC ACTIVE 10 31 82 10 54 30 10 3 11 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 AORTA (AO) ........................................... NUMBER EXAMINED: 1 2 NOT REMARKABLE: 0 1 --MINERALIZATION 11 3724 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- LN, RENAL (RL) ....................................... NUMBER EXAMINED: 2 3 NOT REMARKABLE: 1 0 --DILATATION, SINUSOIDAL --FIBROSIS --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 12 01 13 10 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 5 7 NOT REMARKABLE: 1 1 --DILATATION, SINUSOIDAL --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 34 10 02 HEMATO NEOPLASIA (HN) ................................ NUMBER EXAMINED: 70 5 NOT REMARKABLE: 67 0 --M-LYMPHOMA --M-SARCOMA, HISTIOCYTIC 21 14 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 11 5 NOT REMARKABLE: 1 3 --GALACTOCELE --INFLAMMATION, CHRONIC --MINERALIZATION --B-FIBROADENOMA 91 10 10 20 3725 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 11 5 NOT REMARKABLE: 1 3 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 01 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 TAIL (TI) ............................................ NUMBER EXAMINED: 2 3 NOT REMARKABLE: 0 0 --CYST, EPIDERMAL INCLUSION --HEMORRHAGE --INFLAMMATION, ACUTE --B-FIBROMA --B-PAPILLOMA, SQUAMOUS CELL 01 10 01 10 01 URETER (UE) .......................................... NUMBER EXAMINED: 2 0 NOT REMARKABLE: 0 0 --DILATATION 20 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 3726 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 1 1 NOT REMARKABLE: 0 0 --HEMORRHAGE --M-SARCOMA, OSTEOGENIC 10 01 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 2 1 NOT REMARKABLE: 0 1 --DILATATION, SINUSOIDAL --HEMORRHAGE 20 10 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 2 2 NOT REMARKABLE: 2 0 --INFILTRATE, MACROPHAGE, PIGMENTED 02 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 0 2 NOT REMARKABLE: 0 1 --DILATATION 01 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 0 --M-CARCINOMA 01 3727 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 20 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 0 2 NOT REMARKABLE: 0 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 01 01 02 01 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 0 --FAT NECROSIS 01 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 2 0 NOT REMARKABLE: 0 0 --EDEMA --EXOSTOSES --FIBROSIS --INFLAMMATION, --INFLAMMATION, ACUTE, SYNOVIAL CHRONIC-ACTIVE, SYNOVIAL 20 20 10 10 10 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 3728 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 21 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- NASAL TURBINATE (NT) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 3729 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 22 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- ** TOP OF LIST ** LUNG (LU) ............................................ NUMBER EXAMINED: 60 60 NOT REMARKABLE: 46 43 --HEMORRHAGE --HYPERPLASIA, TYPE II PNEUMOCYTE --INFLAMMATION, CHRONIC --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, MACROPHAGE, PIGMENTED --INFLAMMATION, GRANULOMATOUS --MINERALIZATION --MINERALIZATION, VASCULAR 21 11 22 73 01 10 01 49 KIDNEY (KD) .......................................... NUMBER EXAMINED: 60 60 NOT REMARKABLE: 7 9 --CAST, PROTEINACEOUS --CYST --DILATATION, PELVIC --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFARCT --INFLAMMATION, CHRONIC --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --NEPHROPATHY, CHRONIC PROGRESSIVE --PYELONEPHRITIS 21 20 22 12 10 11 10 02 38 44 20 11 7 34 3730 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 23 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- LIVER (LI) ........................................... NUMBER EXAMINED: 60 60 NOT REMARKABLE: 35 29 --CYST, BILIARY --DEGENERATION, CYSTIC --FIBROSIS --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERPLASIA, BILE DUCT --INFLAMMATION, CHRONIC --NECROSIS, HEPATOCELLULAR --PIGMENT --TELANGIECTASIS --THROMBUS --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 20 12 10 13 37 11 22 57 86 32 10 52 01 14 8 21 01 PANCREAS (PA) ........................................ NUMBER EXAMINED: 59 60 NOT REMARKABLE: 53 51 --ATROPHY, ACINAR --HEMORRHAGE --B-ADENOMA, ISLET CELL 28 30 41 3731 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 24 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- THYROID (TY) ......................................... NUMBER EXAMINED: 60 60 NOT REMARKABLE: 37 42 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --FIBROSIS --HEMORRHAGE --HYPERPLASIA, C-CELL --HYPERPLASIA, FOLLICULAR CELL --INFLAMMATION, CHRONIC --MINERALIZATION --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL 11 16 7 10 01 14 01 20 10 76 11 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 58 1 NOT REMARKABLE: 55 1 --FIBROSIS --HYPERPLASIA 10 20 TESTIS (TE) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 SKIN (SK) ............................................ NUMBER EXAMINED: 60 6 NOT REMARKABLE: 55 0 --CYST, EPIDERMAL INCLUSION 01 3732 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 25 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** SKIN (SK) ............................................ NUMBER EXAMINED: 60 6 NOT REMARKABLE: 55 0 --EROSION --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --PIGMENT --B-BASAL CELL TUMOR --B-FIBROMA --B-KERATOACANTHOMA --B-PAPILLOMA, SQUAMOUS CELL --B-ADENOMA, SEBACEOUS GLAND --M-CARCINOMA, SQUAMOUS CELL 11 11 10 11 01 01 01 10 01 11 10 01 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 60 60 NOT REMARKABLE: 11 12 --FIBROSIS --GALACTOCELE --HEMORRHAGE --HYPERPLASIA, GLANDULAR --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --MINERALIZATION --NECROSIS --PIGMENT 11 47 43 65 13 31 01 9 11 20 42 3733 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 26 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 60 60 NOT REMARKABLE: 11 12 --B-ADENOMA --B-FIBROADENOMA --B-FIBROMA --M-CARCINOMA 52 21 22 10 99 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 60 0 NOT REMARKABLE: 60 0 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 60 0 NOT REMARKABLE: 59 0 --HYPERCELLULARITY, MARROW 10 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 60 0 NOT REMARKABLE: 59 0 --HYPERCELLULARITY, MARROW 10 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 60 0 NOT REMARKABLE: 60 0 3734 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- EYE (EY) ............................................. NUMBER EXAMINED: 60 0 NOT REMARKABLE: 57 0 --FIBROSIS, CORNEAL --INFLAMMATION, ACUTE, CORNEAL --INFLAMMATION, ACUTE, CILIARY --ULCERATION, CORNEAL --VASCULARIZATION, CORNEAL 10 20 10 10 10 BRAIN (BR) ........................................... NUMBER EXAMINED: 60 40 NOT REMARKABLE: 23 0 --COMPRESSION, VENTRAL --DILATATION, VENTRICULAR --HEMORRHAGE --INFLAMMATION, CHRONIC --PIGMENT 36 40 19 23 03 10 01 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 60 1 NOT REMARKABLE: 60 1 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 60 2 NOT REMARKABLE: 59 1 --MINERALIZATION --I-LEIOMYOSARCOMA 10 01 3735 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 28 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 60 1 NOT REMARKABLE: 60 1 HEART (HT) ........................................... NUMBER EXAMINED: 60 2 NOT REMARKABLE: 46 1 --DEGENERATION/NECROSIS --FIBROSIS --INFLAMMATION, CHRONIC --METAPLASIA, CARTILAGE 12 0 71 21 10 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 60 2 NOT REMARKABLE: 54 2 --EDEMA --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 10 30 40 SPLEEN (SP) .......................................... NUMBER EXAMINED: 60 5 NOT REMARKABLE: 56 1 --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED --PIGMENT, INCREASED 34 10 3736 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 29 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- THYMUS (TH) .......................................... NUMBER EXAMINED: 60 2 NOT REMARKABLE: 47 1 --CYST --M-FIBROSARCOMA --M-THYMIC CARCINOMA 12 0 01 10 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 60 0 NOT REMARKABLE: 60 0 TRACHEA (TR) ......................................... NUMBER EXAMINED: 60 0 NOT REMARKABLE: 60 0 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 60 12 NOT REMARKABLE: 12 0 --ANGIECTASIS --DEGENERATION, CYSTIC, CORTICAL --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERTROPHY --MINERALIZATION --ONE EXAMINED --PIGMENT --THROMBUS --VACUOLATION 36 8 19 9 11 44 12 10 10 10 74 11 4 41 3737 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 30 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 60 12 NOT REMARKABLE: 12 0 --B-ADENOMA 20 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 60 4 NOT REMARKABLE: 56 2 --ONE EXAMINED --HYPERPLASIA, MEDULLARY --NECROSIS, MEDULLARY --B-PHEOCHROMOCYTOMA 10 10 01 22 PITUITARY (PI) ....................................... NUMBER EXAMINED: 59 45 NOT REMARKABLE: 9 0 --CYST --HEMORRHAGE --PIGMENT --B-ADENOMA --M-CARCINOMA 46 40 31 85 50 44 01 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 60 0 NOT REMARKABLE: 50 0 --HYPERPLASIA, GLANDULAR --INFLAMMATION, CHRONIC 10 90 3738 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 31 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 60 0 NOT REMARKABLE: 60 0 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 60 3 NOT REMARKABLE: 57 2 --EDEMA --EROSION --INFLAMMATION, ACUTE 11 20 10 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 60 3 NOT REMARKABLE: 54 0 --ACANTHOSIS --EDEMA --EROSION --HEMORRHAGE --HYPERKERATOSIS, NONGLANDULAR --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION MUCOSA 01 52 32 10 01 31 11 20 DUODENUM (DU) ........................................ NUMBER EXAMINED: 58 0 NOT REMARKABLE: 58 0 3739 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 32 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- JEJUNUM (JE) ......................................... NUMBER EXAMINED: 58 0 NOT REMARKABLE: 58 0 ILEUM (IL) ........................................... NUMBER EXAMINED: 55 0 NOT REMARKABLE: 55 0 CECUM (CE) ........................................... NUMBER EXAMINED: 60 3 NOT REMARKABLE: 59 1 --EDEMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 10 02 COLON (CO) ........................................... NUMBER EXAMINED: 60 0 NOT REMARKABLE: 60 0 RECTUM (RE) .......................................... NUMBER EXAMINED: 60 1 NOT REMARKABLE: 53 0 --PARASITISM --M-LEIOMYOSARCOMA 70 01 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 60 15 NOT REMARKABLE: 58 15 --HYPERPLASIA, TRANSITIONAL CELL 10 3740 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 33 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 60 15 NOT REMARKABLE: 58 15 --INFLAMMATION, CHRONIC 20 UTERUS (UT) .......................................... NUMBER EXAMINED: 60 10 NOT REMARKABLE: 48 0 --DILATATION --HYPERPLASIA, CYSTIC, ENDOMETRIAL --INFLAMMATION, ACUTE --SCLEROSIS --B-POLYP, ENDOMETRIAL, STROMAL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 85 36 10 01 12 10 OVARY (OV) ........................................... NUMBER EXAMINED: 60 12 NOT REMARKABLE: 51 4 --CYST --INFLAMMATION, CHRONIC --B-GRANULOSA/THECA CELL TUMOR 88 10 10 CERVIX (CV) .......................................... NUMBER EXAMINED: 60 2 NOT REMARKABLE: 59 0 --DILATATION --HYPERTROPHY, MUSCULAR --INFLAMMATION, ACUTE 01 01 01 3741 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 34 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** CERVIX (CV) .......................................... NUMBER EXAMINED: 60 2 NOT REMARKABLE: 59 0 --B-LEIOMYOMA --M-SARCOMA, ENDOMETRIAL STROMAL 10 01 VAGINA (VA) .......................................... NUMBER EXAMINED: 60 8 NOT REMARKABLE: 59 7 --INFLAMMATION, ACUTE --N-SARCOMA, ENDOMETRIAL STROMAL 11 01 PROSTATE (PR) ........................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 DEATH COMMENT (DC) ................................... NUMBER EXAMINED: 60 60 NOT REMARKABLE: 0 0 --ENDOMETRIAL STROMAL SARCOMA 01 3742 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 35 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** DEATH COMMENT (DC) ................................... NUMBER EXAMINED: 60 60 NOT REMARKABLE: 0 0 --FRACTURED CALVARIUM --HISTIOCYTIC SARCOMA --MALIGNANT LYMPHOMA --MAMMARY CARCINOMA --MAMMARY FIBROADENOMA --ORAL EROSION --OSTEOMA --PITUITARY ADENOMA --PITUITARY CARCINOMA --PYELONEPHRITIS --SCHEDULED SACRIFICE --UNDETERMINED 10 01 01 03 32 10 10 29 25 01 10 23 25 11 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 12 11 NOT REMARKABLE: 4 6 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED 54 31 10 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 16 15 NOT REMARKABLE: 11 11 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC 14 40 3743 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 36 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 16 15 NOT REMARKABLE: 11 11 --INFILTRATE, MACROPHAGE, VACUOLATED 12 FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 2 6 NOT REMARKABLE: 0 0 --CYST --EDEMA --EROSION --EXOSTOSES --FIBROSIS --INFLAMMATION, --INFLAMMATION, --PIGMENT ACUTE CHRONIC ACTIVE 01 01 23 02 23 22 02 01 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 3 1 NOT REMARKABLE: 1 1 --HYPERPLASIA, LYMPHOCYTIC --N-MAMMARY CARCINOMA 10 10 AORTA (AO) ........................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 3744 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 37 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- LN, RENAL (RL) ....................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 1 4 NOT REMARKABLE: 1 4 HEMATO NEOPLASIA (HN) ................................ NUMBER EXAMINED: 70 2 NOT REMARKABLE: 69 0 --M-LYMPHOMA --M-SARCOMA, HISTIOCYTIC 01 11 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 1 0 NOT REMARKABLE: 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 10 TAIL (TI) ............................................ NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 0 --EROSION --INFLAMMATION, ACUTE 01 01 3745 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 38 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** TAIL (TI) ............................................ NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 0 --M-FIBROSARCOMA 01 URETER (UE) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 CAVITY, ORAL (OC) .................................... NUMBER EXAMINED: 1 0 NOT REMARKABLE: 0 0 --EDEMA --EROSION --HEMORRHAGE --INFLAMMATION, ACUTE 10 10 10 10 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 1 0 NOT REMARKABLE: 0 0 --CYST, KERATIN --FRACTURE --HEMORRHAGE --INFLAMMATION, ACUTE 10 10 10 10 3746 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 39 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 4 1 NOT REMARKABLE: 0 0 --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 21 20 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 1 0 NOT REMARKABLE: 1 0 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 1 1 NOT REMARKABLE: 0 0 --B-LIPOMA --M-LIPOSARCOMA 01 10 3747 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 40 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ------------------------------------------------------------------------ -=- -=- JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 0 --EXOSTOSES --FIBROSIS --INFLAMMATION, CHRONIC, SYNOVIAL 01 01 01 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 NASAL TURBINATE (NT) ................................. NUMBER EXAMINED: 1 0 NOT REMARKABLE: 0 0 --B-OSTEOMA 10 3748 Covance 6329-212 TABLE 37 Incidence of Severity of Selected Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 10 10 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ** TOP OF LIST ** LIVER (LI) ........................................... NUMBER EXAMINED: 10 10 10 10 --CYST -> 10 10 10 10 TL> 10 10 10 10 --CYST, BILIARY -> 10 10 10 10 TL> 10 10 10 10 MN> 0.0 0.0 0.0 0.0 --DEGENERATION, CYSTIC -> 10 9 10 10 3> 0 1 0 0 TL> 10 10 10 10 MN> 0.0 0.3 0.0 0.0 --FIBROSIS -> 10 10 10 10 TL> 10 10 10 10 MN> 0.0 0.0 0.0 0.0 --FIBROSIS, CAPSULAR -> 10 10 10 10 TL> 10 10 10 10 MN> 0.0 0.0 0.0 0.0 --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC -> 10 10 10 10 TL> 10 10 10 10 MN> 0.0 0.0 0.0 0.0 3749 Covance 6329-212 TABLE 37 Incidence of Severity of Selected Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 10 10 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 10 10 10 10 --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC -> 10 10 10 10 TL> 10 10 10 10 MN> 0.0 0.0 0.0 0.0 --HEMATOPOIESIS, EXTRAMEDULLARY -> 10 10 10 10 TL> 10 10 10 10 MN> 0.0 0.0 0.0 0.0 --HEMORRHAGE -> 8 10 9 10 1> 1 0 0 0 2> 1 0 1 0 TL> 10 10 10 10 MN> 0.3 0.0 0.2 0.0 --HYPERPLASIA, BILE DUCT -> 10 10 10 9 1> 0 0 0 1 TL> 10 10 10 10 MN> 0.0 0.0 0.0 0.1 --INFILTRATE, MAST CELLS -> 10 10 10 10 TL> 10 10 10 10 MN> 0.0 0.0 0.0 0.0 3750 Covance 6329-212 TABLE 37 Incidence of Severity of Selected Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 10 10 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 10 10 10 10 --INFLAMMATION, CHRONIC -> 9 8 10 10 1> 1 2 0 0 TL> 10 10 10 10 MN> 0.1 0.2 0.0 0.0 --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) -> 9 10 10 10 4> 1 0 0 0 TL> 10 10 10 10 MN> 0.4 0.0 0.0 0.0 --NECROSIS, HEPATOCELLULAR -> 9 10 9 10 2> 0 0 1 0 4> 1 0 0 0 TL> 10 10 10 10 MN> 0.4 0.0 0.2 0.0 --PIGMENT -> 10 10 10 10 TL> 10 10 10 10 MN> 0.0 0.0 0.0 0.0 --TELANGIECTASIS -> 10 10 10 10 TL> 10 10 10 10 MN> 0.0 0.0 0.0 0.0 3751 Covance 6329-212 TABLE 37 Incidence of Severity of Selected Microscopic Observations Covance 6329-228 3M T-6316.1 Week 53 Sacrifice PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 10 10 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 10 10 10 10 --THROMBUS -> 10 10 10 10 TL> 10 10 10 10 MN> 0.0 0.0 0.0 0.0 --VACUOLATION, HEPATOCELLULAR -> 10 8 10 10 1> 0 1 0 0 2> 0 1 0 0 TL> 10 10 10 10 MN> 0.0 0.3 0.0 0.0 --B-ADENOMA, HEPATOCELLULAR -> 10 9 10 10 +> 0 1 0 0 TL> 10 10 10 10 --B-HEMANGIOMA -> 10 10 10 10 TL> 10 10 10 10 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) ** END OF LIST ** -> 10 10 10 10 TL> 10 10 10 10 3752 Covance 6329-212 TABLE 38 Incidence of Severity of Selected Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 11 20 13 15 ------------------------------------------------------------------------ -=- -=- -=- -=- ** TOP OF LIST ** LIVER (LI) ........................................... NUMBER EXAMINED: 11 20 13 15 --CYST -> 11 20 13 15 TL> 11 20 13 15 --CYST, BILIARY -> 11 20 12 15 3> 0 0 1 0 TL> 11 20 13 15 MN> 0.0 0.0 0.2 0.0 --DEGENERATION, CYSTIC -> 9 16 12 14 1> 1 0 0 0 2> 0 3 0 0 3> 1 1 1 1 TL> 11 20 13 15 MN> 0.4 0.5 0.2 0.2 --FIBROSIS -> 11 20 12 15 5> 0 0 1 0 TL> 11 20 13 15 MN> 0.0 0.0 0.4 0.0 --FIBROSIS, CAPSULAR -> 11 19 13 15 2> 0 1 0 0 TL> 11 20 13 15 MN> 0.0 0.1 0.0 0.0 3753 Covance 6329-212 TABLE 38 Incidence of Severity of Selected Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 11 20 13 15 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 11 20 13 15 --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC -> 11 19 12 13 2> 0 0 1 1 3> 0 1 0 1 TL> 11 20 13 15 MN> 0.0 0.2 0.2 0.3 --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC -> 10 14 10 10 1> 0 0 0 1 2> 0 2 0 1 3> 0 4 3 1 4> 1 0 0 2 TL> 11 20 13 15 MN> 0.4 0.8 0.7 0.9 --HEMATOPOIESIS, EXTRAMEDULLARY -> 11 20 13 15 TL> 11 20 13 15 MN> 0.0 0.0 0.0 0.0 --HEMORRHAGE -> 10 19 13 15 2> 1 0 0 0 3> 0 1 0 0 TL> 11 20 13 15 MN> 0.2 0.2 0.0 0.0 3754 Covance 6329-212 TABLE 38 Incidence of Severity of Selected Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 11 20 13 15 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 11 20 13 15 --HYPERPLASIA, BILE DUCT -> 7 15 11 13 1> 4 5 1 2 2> 0 0 1 0 TL> 11 20 13 15 MN> 0.4 0.3 0.2 0.1 --INFILTRATE, MAST CELLS -> 11 20 13 15 TL> 11 20 13 15 MN> 0.0 0.0 0.0 0.0 --INFLAMMATION, CHRONIC -> 5 10 9 11 1> 6 10 3 4 2> 0 0 1 0 TL> 11 20 13 15 MN> 0.5 0.5 0.4 0.3 --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) -> 11 20 13 15 TL> 11 20 13 15 MN> 0.0 0.0 0.0 0.0 --NECROSIS, HEPATOCELLULAR -> 11 20 12 15 5> 0 0 1 0 TL> 11 20 13 15 MN> 0.0 0.0 0.4 0.0 3755 Covance 6329-212 TABLE 38 Incidence of Severity of Selected Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 11 20 13 15 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 11 20 13 15 --PIGMENT -> 11 20 13 15 TL> 11 20 13 15 MN> 0.0 0.0 0.0 0.0 --TELANGIECTASIS -> 11 20 10 13 1> 0 0 3 2 TL> 11 20 13 15 MN> 0.0 0.0 0.2 0.1 --THROMBUS -> 11 20 13 14 4> 0 0 0 1 TL> 11 20 13 15 MN> 0.0 0.0 0.0 0.3 --VACUOLATION, HEPATOCELLULAR -> 11 15 11 14 1> 0 1 0 1 2> 0 4 2 0 TL> 11 20 13 15 MN> 0.0 0.5 0.3 0.1 --B-ADENOMA, HEPATOCELLULAR -> 10 19 12 15 +> 1 1 1 0 TL> 11 20 13 15 --B-HEMANGIOMA -> 11 20 13 15 TL> 11 20 13 15 3756 Covance 6329-212 TABLE 38 Incidence of Severity of Selected Microscopic Observations Covance 6329-228 3M T-6316.1 Terminal Sacrifice PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 11 20 13 15 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 11 20 13 15 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) -> 11 18 13 15 +> 0 2 0 0 TL> 11 20 13 15 ** END OF LIST ** 3757 Covance 6329-212 TABLE 39 Incidence of Severity of Selected Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- ** TOP OF LIST ** LIVER (LI) ........................................... NUMBER EXAMINED: 39 30 37 35 --CYST -> 37 30 37 35 P> 2 0 0 0 TL> 39 30 37 35 --CYST, BILIARY -> 39 30 36 35 3> 0 0 1 0 TL> 39 30 37 35 MN> 0.0 0.0 0.1 0.0 --DEGENERATION, CYSTIC -> 36 26 37 34 1> 1 0 0 1 3> 2 2 0 0 4> 0 2 0 0 TL> 39 30 37 35 MN> 0.2 0.5 0.0 0.0 --FIBROSIS -> 39 30 37 35 TL> 39 30 37 35 MN> 0.0 0.0 0.0 0.0 --FIBROSIS, CAPSULAR -> 39 30 37 35 TL> 39 30 37 35 MN> 0.0 0.0 0.0 0.0 3758 Covance 6329-212 TABLE 39 Incidence of Severity of Selected Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 39 30 37 35 --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC -> 39 30 37 34 3> 0 0 0 1 TL> 39 30 37 35 MN> 0.0 0.0 0.0 0.1 --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC -> 36 27 37 33 2> 1 0 0 1 3> 2 3 0 1 TL> 39 30 37 35 MN> 0.2 0.3 0.0 0.1 --HEMATOPOIESIS, EXTRAMEDULLARY -> 37 29 36 34 1> 2 1 1 0 2> 0 0 0 1 TL> 39 30 37 35 MN> 0.1 0.0 0.0 0.1 --HEMORRHAGE -> 37 29 36 33 1> 0 0 0 2 2> 1 1 1 0 3> 1 0 0 0 TL> 39 30 37 35 MN> 0.1 0.1 0.1 0.1 3759 Covance 6329-212 TABLE 39 Incidence of Severity of Selected Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 39 30 37 35 --HYPERPLASIA, BILE DUCT -> 36 27 34 31 1> 2 3 2 4 3> 1 0 1 0 TL> 39 30 37 35 MN> 0.1 0.1 0.1 0.1 --INFILTRATE, MAST CELLS -> 39 29 37 35 2> 0 1 0 0 TL> 39 30 37 35 MN> 0.0 0.1 0.0 0.0 --INFLAMMATION, CHRONIC -> 29 27 33 33 1> 10 2 4 2 3> 0 1 0 0 TL> 39 30 37 35 MN> 0.3 0.2 0.1 0.1 --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) -> 38 29 37 35 4> 1 1 0 0 TL> 39 30 37 35 MN> 0.1 0.1 0.0 0.0 3760 Covance 6329-212 TABLE 39 Incidence of Severity of Selected Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 39 30 37 35 --NECROSIS, HEPATOCELLULAR -> 38 27 36 33 1> 0 0 1 0 2> 0 1 0 1 3> 1 2 0 1 TL> 39 30 37 35 MN> 0.1 0.3 0.0 0.1 --PIGMENT -> 39 30 36 35 1> 0 0 1 0 TL> 39 30 37 35 MN> 0.0 0.0 0.0 0.0 --TELANGIECTASIS -> 39 30 35 35 1> 0 0 1 0 5> 0 0 1 0 TL> 39 30 37 35 MN> 0.0 0.0 0.2 0.0 --THROMBUS -> 39 30 37 35 TL> 39 30 37 35 MN> 0.0 0.0 0.0 0.0 3761 Covance 6329-212 TABLE 39 Incidence of Severity of Selected Microscopic Observations Covance 6329-228 3M T-6316.1 Unscheduled Deaths PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 39 30 37 35 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 39 30 37 35 --VACUOLATION, HEPATOCELLULAR -> 25 27 25 28 1> 2 0 2 2 2> 3 1 5 2 3> 6 1 3 0 4> 1 1 1 3 5> 2 0 1 0 TL> 39 30 37 35 MN> 1.0 0.3 0.8 0.5 --B-ADENOMA, HEPATOCELLULAR -> 35 28 36 34 +> 4 2 1 1 TL> 39 30 37 35 --B-HEMANGIOMA -> 39 30 37 35 TL> 39 30 37 35 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) ** END OF LIST ** -> 38 28 37 34 +> 1 2 0 1 TL> 39 30 37 35 3762 Covance 6329-212 TABLE 40 Incidence of Severity of Selected Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- ** TOP OF LIST ** LIVER (LI) ........................................... NUMBER EXAMINED: 60 60 60 60 --CYST -> 58 60 60 60 P> 2 0 0 0 TL> 60 60 60 60 --CYST, BILIARY -> 60 60 58 60 3> 0 0 2 0 TL> 60 60 60 60 MN> 0.0 0.0 0.1 0.0 --DEGENERATION, CYSTIC -> 55 51 59 58 1> 2 0 0 1 2> 0 3 0 0 3> 3 4 1 1 4> 0 2 0 0 TL> 60 60 60 60 MN> 0.2 0.4 0.1 0.1 --FIBROSIS -> 60 60 59 60 5> 0 0 1 0 TL> 60 60 60 60 MN> 0.0 0.0 0.1 0.0 --FIBROSIS, CAPSULAR -> 60 59 60 60 2> 0 1 0 0 TL> 60 60 60 60 MN> 0.0 0.0 0.0 0.0 3763 Covance 6329-212 TABLE 40 Incidence of Severity of Selected Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 60 60 60 60 --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC -> 60 59 59 57 2> 0 0 1 1 3> 0 1 0 2 TL> 60 60 60 60 MN> 0.0 0.1 0.0 0.1 --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC -> 56 51 57 53 1> 0 0 0 1 2> 1 2 0 2 3> 2 7 3 2 4> 1 0 0 2 TL> 60 60 60 60 MN> 0.2 0.4 0.2 0.3 --HEMATOPOIESIS, EXTRAMEDULLARY -> 58 59 59 59 1> 2 1 1 0 2> 0 0 0 1 TL> 60 60 60 60 MN> 0.0 0.0 0.0 0.0 --HEMORRHAGE -> 55 58 58 58 1> 1 0 0 2 2> 3 1 2 0 3> 1 1 0 0 TL> 60 60 60 60 MN> 0.2 0.1 0.1 0.0 3764 Covance 6329-212 TABLE 40 Incidence of Severity of Selected Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 60 60 60 60 --HYPERPLASIA, BILE DUCT -> 53 52 55 53 1> 6 8 3 7 2> 0 0 1 0 3> 1 0 1 0 TL> 60 60 60 60 MN> 0.2 0.1 0.1 0.1 --INFILTRATE, MAST CELLS -> 60 59 60 60 2> 0 1 0 0 TL> 60 60 60 60 MN> 0.0 0.0 0.0 0.0 --INFLAMMATION, CHRONIC -> 43 45 52 54 1> 17 14 7 6 2> 0 0 1 0 3> 0 1 0 0 TL> 60 60 60 60 MN> 0.3 0.3 0.2 0.1 --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) -> 58 59 60 60 4> 2 1 0 0 TL> 60 60 60 60 MN> 0.1 0.1 0.0 0.0 3765 Covance 6329-212 TABLE 40 Incidence of Severity of Selected Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 60 60 60 60 --NECROSIS, HEPATOCELLULAR -> 58 57 57 58 1> 0 0 1 0 2> 0 1 1 1 3> 1 2 0 1 4> 1 0 0 0 5> 0 0 1 0 TL> 60 60 60 60 MN> 0.1 0.1 0.1 0.1 --PIGMENT -> 60 60 59 60 1> 0 0 1 0 TL> 60 60 60 60 MN> 0.0 0.0 0.0 0.0 --TELANGIECTASIS -> 60 60 55 58 1> 0 0 4 2 5> 0 0 1 0 TL> 60 60 60 60 MN> 0.0 0.0 0.2 0.0 --THROMBUS -> 60 60 60 59 4> 0 0 0 1 TL> 60 60 60 60 MN> 0.0 0.0 0.0 0.1 3766 Covance 6329-212 TABLE 40 Incidence of Severity of Selected Microscopic Observations Covance 6329-228 3M T-6316.1 All Animals PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 70 70 70 70 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 60 60 60 60 --VACUOLATION, HEPATOCELLULAR -> 46 50 46 52 1> 2 2 2 3 2> 3 6 7 2 3> 6 1 3 0 4> 1 1 1 3 5> 2 0 1 0 TL> 60 60 60 60 MN> 0.7 0.4 0.6 0.3 --B-ADENOMA, HEPATOCELLULAR -> 55 56 58 59 +> 5 4 2 1 TL> 60 60 60 60 --B-HEMANGIOMA -> 60 60 60 60 TL> 60 60 60 60 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) ** END OF LIST ** -> 59 56 60 59 +> 1 4 0 1 TL> 60 60 60 60 3767 APPENDIX 1 Protocol Deviations Covance 6329-212 Covance 6329-228 3M T-6316.1 3768 Protocol Deviations Protocol. Test Material. Purity. 98.1% N-EtFOSE (w/w). Covance 6329-212 Covance 6329-228 3M T-6316.1 Actual Procedure. The purity of the test material, as determined by Centre Analytical Laboratories, Inc. on November 26, 2000, was 97.4%. Protocol. Dosing Procedures. Method of Administration. Animals will receive test diet for at least 104 weeks. Actual Procedure. Animal No. C92392, Group 8 female, was found without feed on September 21, 1998. Protocol. Observation of Animals. Clinical Observations. Animals will be observeed twice daily (a.m. and p.m.). Per standard operating procedure, p.m. checks must be done at least 6 hours after the a.m. check and not before 12:00 p.m. Actual Procedure. The p.m. room check was done at 11:52 on June 31, 1998. The p.m. room check was done 17minutes early on January 25, 2000. Protocol. Observation of Animals. Body Weights. Body weights will be taken weekly for Weeks 1 through 17. Actual Procedure. The body weight taken on May 25, 1998, for Animal No. C92447. Group 9 female, was excluded due to a weighing error. The body weight taken for Animal No. C92345, Group 8 female, was excluded due to a weighing error. Protocol. Observation of Animals. Food Consumption. Animals will have food consumption recorded weekly for Weeks 1 through 16 and once every 4 weeks thereafter. 3769 Protocol Deviations (Continued) Covance 6329-212 Covance 6329-228 3M T-6316.1 Actual Procedure. Food consumption values were excluded for Week 14 for animals where empty feeder weights were not documented. Documentation of spilled feed may have not been recorded during Week 76, therefore food consumption data may be in error. Protocol. Postmortem Procedures. Palmitoyl-CoA Oxidase Tissue Collection and Analyses. At the Week 14 sacrifice, a sample of the right lateral lobe will be flash-frozen in liquid nitrogen. Actual Procedure. Flash-freezing of the liver at Week 14 was not documented. Protocol. Postmortem Procedures. Organ Weights. Liver will be weighed at the Week 53 interim sacrifice. Actual Procedure. The liver was not weighed for Animal No. C92326, Group 9 male. Protocol. Postmortem Procedures. Tissue Preservation. A selected list of tissues will be collected from each animal and stored in 10% neutral-buffered formalin. Actual Procedure. Selected tissues to be held and not examined microscopically for animals in Group 9 were trimmed. These tissues were returned to wet tissue and not processed to slides. Protocol. Postmortem Procedures. Histopathology. Selected tissues from each animal in Groups 8 and 9 will be examined microscopically. 3770 Protocol Deviations (Continued) Covance 6329-212 Covance 6329-228 3M T-6316.1 Actual Procedure. Some tissues, required by protocol, were not available for histopathologuc examination. Missing tissues are listed with appropriate comments in the pathology data sheets for individual animals. Summary tables do not include them as having been examined. These deviations are not expected to have affected the results of the study. 3771 Covance 6329-212 Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Author: Peter J. Thomford, PhD Study Completion Date: December 13, 2001 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-228 Sponsor Study Identification: 3M T-6316.1 Volume X of XII Page 3772 of 4873 Covance 6329-212 Covance 6329-228 3M T-6316.1 VOLUME X OF XII CONTENTS Page APPENDIX 2............................................................................................................ 3774 Individual Animal Fate Data.................................................................................. 3775 Individual Clinical Observations............................................................................ 3784 APPENDIX 3............................................................................................................ 4017 Individual Body Weight Data (g)........................................................................... 4018 APPENDIX 4............................................................................................................ 4051 Individual Food Consumption Data (g).................................................................. 4052 Individual Test Material Consumption Data (mg/kg/day)....................................... 4085 APPENDIX 5............................................................................................................ 4105 Individual Clinical Hematology Data..................................................................... 4106 Individual Clinical Chemistry Data........................................................................ 4124 Individual Clinical Urinalysis Data........................................................................ 4140 Individual Urine Chemistry Data ........................................................................... 4158 3773 APPENDIX 2 Individual Animal Fate Data Individual Clinical Observations Covance 6329-212 Covance 6329-228 3M T-6316.1 3774 Covance 6329-212 APPENDIX 2 Individual Animal Fate Data Covance 6329-228 3M T-6316.1 PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92191 C92192 C92193 C92194 C92195 C92196 C92197 C92198 C92199 C92200 C92201 C92202 C92203 C92204 C92205 C92206 C92207 C92208 C92209 C92210 C92211 C92212 C92213 C92214 C92215 C92216 C92217 C92218 C92219 C92220 C92221 C92222 C92223 C92224 DOSE GROUP ----- 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- M 1 T M 3 1 T D 3 M M D T D 3 M 1 T T M 3 M M M M 1 M T M D M T 3 D TYPE OF DEATH ----------UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH FIRST INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE FIRST INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH DATE OF DEATH -------03/27/00 04/28/98 04/04/00 03/11/00 04/07/99 04/28/98 04/04/00 06/19/98 04/07/99 09/21/99 01/27/00 11/25/99 04/04/00 10/27/99 04/07/99 09/14/99 04/28/98 04/04/00 04/04/00 02/23/00 04/07/99 03/27/00 03/27/99 11/15/99 09/14/99 04/28/98 10/04/99 04/04/00 12/03/99 09/25/99 01/10/00 04/04/00 04/07/99 03/21/00 DAY OF STUDY ------722 23 730 706 367 23 730 75 367 534 662 599 730 570 367 527 23 730 730 689 367 722 356 589 527 23 547 730 607 538 645 730 367 716 WEEK OF STUDY ------104 4 105 101 53 4 105 11 53 77 95 86 105 82 53 76 4 105 105 99 53 104 51 85 76 4 79 105 87 77 93 105 53 103 3775 Covance 6329-212 APPENDIX 2 Individual Animal Fate Data Covance 6329-228 3M T-6316.1 PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92225 C92226 C92227 C92228 C92229 C92230 C92231 C92232 C92233 C92234 C92235 C92236 C92237 C92238 C92239 C92240 C92241 C92242 C92243 C92244 C92245 C92246 C92247 C92248 C92249 C92250 C92251 C92252 C92253 C92254 C92255 C92256 C92257 C92258 DOSE GROUP ----- 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- M M M M D 3 T M T M M D M 3 T D M M M D M 3 3 M D M M T 3 M 2 2 2 2 TYPE OF DEATH ----------UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH SECOND INTERIM SACRIFICE SECOND INTERIM SACRIFICE SECOND INTERIM SACRIFICE SECOND INTERIM SACRIFICE DATE OF DEATH -------08/12/99 12/27/99 03/17/00 06/25/99 11/26/99 04/07/99 04/04/00 02/28/00 04/04/00 11/15/99 02/11/00 12/20/99 03/11/00 04/07/99 04/04/00 11/04/99 01/10/00 07/14/99 08/03/99 07/02/99 07/25/99 04/07/99 04/07/99 07/26/99 08/17/99 01/02/00 11/08/99 04/04/00 04/07/99 11/29/99 07/07/98 07/07/98 07/07/98 07/07/98 DAY OF STUDY ------494 631 712 446 600 367 730 694 730 589 677 624 706 367 730 578 645 465 485 453 476 367 367 477 499 637 582 730 367 603 93 93 93 93 WEEK OF STUDY ------71 91 102 64 86 53 105 100 105 85 97 90 101 53 105 83 93 67 70 65 68 53 53 69 72 91 84 105 53 87 14 14 14 14 3776 Covance 6329-212 APPENDIX 2 Individual Animal Fate Data Covance 6329-228 3M T-6316.1 PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92259 C92260 C92261 C92262 C92263 C92264 C92265 C92266 C92267 C92268 C92269 C92270 C92271 C92272 C92273 C92274 C92275 C92276 C92277 C92278 C92279 C92280 C92281 C92282 C92283 C92284 C92285 C92286 C92287 C92288 C92289 C92290 C92291 C92292 DOSE GROUP ----- 8 8 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- 1 2 T M T 1 D T 3 M D T D M D 3 M D M T M 1 T T T 1 M 3 3 3 T M D M TYPE OF DEATH ----------SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------FIRST INTERIM SACRIFICE SECOND INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE THIRD INTERIM SACRIFICE THIRD INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH DATE OF DEATH -------04/28/98 07/07/98 04/04/00 03/27/00 04/04/00 04/28/98 10/16/99 04/04/00 04/07/99 02/28/00 08/24/99 04/04/00 12/21/99 11/16/99 01/24/99 04/07/99 03/27/00 08/28/99 05/25/99 04/04/00 12/13/99 04/28/98 04/04/00 04/04/00 04/04/00 04/28/98 09/14/99 04/07/99 04/07/99 04/07/99 04/04/00 06/17/99 02/07/00 07/12/99 DAY OF STUDY ------23 93 730 722 730 23 559 730 367 694 506 730 625 590 294 367 722 510 415 730 617 23 730 730 730 23 527 367 367 367 730 438 673 463 WEEK OF STUDY ------4 14 105 104 105 4 80 105 53 100 73 105 90 85 42 53 104 73 60 105 89 4 105 105 105 4 76 53 53 53 105 63 97 67 3777 Covance 6329-212 APPENDIX 2 Individual Animal Fate Data Covance 6329-228 3M T-6316.1 PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92293 C92294 C92295 C92296 C92297 C92298 C92299 C92300 C92301 C92302 C92303 C92304 C92305 C92306 C92307 C92308 C92309 C92310 C92311 C92312 C92313 C92314 C92315 C92316 C92317 C92318 C92319 C92320 C92321 C92322 C92323 C92324 C92325 C92326 DOSE GROUP ----- 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- D M T M T M T 1 D M 3 M M D M T T D T M D M 3 M 3 T T 3 T T T 3 2 2 TYPE OF DEATH ----------UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE THIRD INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE THIRD INTERIM SACRIFICE SECOND INTERIM SACRIFICE SECOND INTERIM SACRIFICE DATE OF DEATH -------01/18/99 02/07/00 04/04/00 09/12/99 04/04/00 07/19/99 04/04/00 04/28/98 12/01/99 02/02/00 04/07/99 01/31/00 06/17/99 03/27/00 03/12/00 04/04/00 04/04/00 08/25/99 04/04/00 06/28/99 02/08/00 01/14/00 04/07/99 03/07/00 04/07/99 04/04/00 04/04/00 04/07/99 04/04/00 04/04/00 04/04/00 04/07/99 07/07/98 07/07/98 DAY OF STUDY ------288 673 730 525 730 470 730 23 605 668 367 666 438 722 707 730 730 507 730 449 674 649 367 702 367 730 730 367 730 730 730 367 93 93 WEEK OF STUDY ------42 97 105 75 105 68 105 4 87 96 53 96 63 104 101 105 105 73 105 65 97 93 53 101 53 105 105 53 105 105 105 53 14 14 3778 Covance 6329-212 APPENDIX 2 Individual Animal Fate Data Covance 6329-228 3M T-6316.1 PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92327 C92328 C92329 C92330 C92331 C92332 C92333 C92334 C92335 C92336 C92337 C92338 C92339 C92340 C92341 C92342 C92343 C92344 C92345 C92346 C92347 C92348 C92349 C92350 C92351 C92352 C92353 C92354 C92355 C92356 C92357 C92358 C92359 C92360 DOSE GROUP ----- 9 9 9 9 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 SEX ------ MALE MALE MALE MALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- 2 2 1 2 M 1 T T M M M M D M 3 M M M D M M T D M T 1 M T 1 3 3 T D T TYPE OF DEATH ----------SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------SECOND INTERIM SACRIFICE SECOND INTERIM SACRIFICE FIRST INTERIM SACRIFICE SECOND INTERIM SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE FIRST INTERIM SACRIFICE THIRD INTERIM SACRIFICE THIRD INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE DATE OF DEATH -------07/07/98 07/07/98 04/28/98 07/07/98 01/06/00 04/28/98 04/04/00 04/04/00 05/29/98 10/27/99 10/26/99 08/02/99 01/17/00 10/05/99 04/07/99 03/15/99 05/10/99 03/27/00 10/28/98 06/14/99 03/01/99 04/04/00 09/28/99 01/21/00 04/04/00 04/28/98 02/01/00 04/04/00 04/28/98 04/07/99 04/07/99 04/04/00 01/13/00 04/04/00 DAY OF STUDY ------93 93 23 93 641 23 730 730 54 570 569 484 652 548 367 344 400 722 206 435 330 730 541 656 730 23 667 730 23 367 367 730 648 730 WEEK OF STUDY ------14 14 4 14 92 4 105 105 8 82 82 70 94 79 53 50 58 104 30 63 48 105 78 94 105 4 96 105 4 53 53 105 93 105 3779 Covance 6329-212 APPENDIX 2 Individual Animal Fate Data Covance 6329-228 3M T-6316.1 PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92361 C92362 C92363 C92364 C92365 C92366 C92367 C92368 C92369 C92370 C92371 C92372 C92373 C92374 C92375 C92376 C92377 C92378 C92379 C92380 C92381 C92382 C92383 C92384 C92385 C92386 C92387 C92388 C92389 C92390 C92391 C92392 C92393 C92394 DOSE GROUP ----- 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- M T M M 3 1 T M T M D 3 1 T M 3 D M D M M M T M M T M 3 3 3 D M M M TYPE OF DEATH ----------UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE FIRST INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE FIRST INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE THIRD INTERIM SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH DATE OF DEATH -------03/07/00 04/04/00 11/01/99 06/17/99 04/07/99 04/28/98 04/04/00 03/17/00 04/04/00 03/02/00 12/15/99 04/07/99 04/28/98 04/04/00 12/01/99 04/07/99 05/03/99 08/26/99 08/16/99 10/18/99 07/29/99 05/03/99 04/04/00 12/10/99 01/31/00 04/04/00 08/31/99 04/07/99 04/07/99 04/07/99 01/12/00 06/21/99 01/18/00 11/15/99 DAY OF STUDY ------702 730 575 438 367 23 730 712 730 697 619 367 23 730 605 367 393 508 498 561 480 393 730 614 666 730 513 367 367 367 647 442 653 589 WEEK OF STUDY ------101 105 83 63 53 4 105 102 105 100 89 53 4 105 87 53 57 73 72 81 69 57 105 88 96 105 74 53 53 53 93 64 94 85 3780 Covance 6329-212 APPENDIX 2 Individual Animal Fate Data Covance 6329-228 3M T-6316.1 PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92395 C92396 C92397 C92398 C92399 C92400 C92401 C92402 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 C92412 C92413 C92414 C92415 C92416 C92417 C92418 C92419 C92420 C92421 C92422 C92423 C92424 C92425 C92426 C92427 C92428 DOSE GROUP ----- 8 8 8 8 8 8 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- 3 2 2 2 2 2 M 1 D M M 3 3 M M T 3 T M M D T M 3 T M T 3 T 3 M T M M TYPE OF DEATH ----------SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------THIRD INTERIM SACRIFICE SECOND INTERIM SACRIFICE SECOND INTERIM SACRIFICE SECOND INTERIM SACRIFICE SECOND INTERIM SACRIFICE SECOND INTERIM SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE THIRD INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE THIRD INTERIM SACRIFICE TERMINAL SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH DATE OF DEATH -------04/07/99 07/07/98 07/07/98 07/07/98 07/07/98 07/07/98 01/06/00 04/28/98 03/27/99 10/23/99 02/20/00 04/07/99 04/07/99 03/27/00 02/25/00 04/04/00 04/07/99 04/04/00 03/15/00 06/14/99 12/15/99 04/04/00 01/18/00 04/07/99 04/04/00 08/23/99 04/04/00 04/07/99 04/04/00 04/07/99 02/28/00 04/04/00 06/14/99 06/07/99 DAY OF STUDY ------367 93 93 93 93 93 641 23 356 566 686 367 367 722 691 730 367 730 710 435 619 730 653 367 730 505 730 367 730 367 694 730 435 428 WEEK OF STUDY ------53 14 14 14 14 14 92 4 51 81 98 53 53 104 99 105 53 105 102 63 89 105 94 53 105 73 105 53 105 53 100 105 63 62 3781 Covance 6329-212 APPENDIX 2 Individual Animal Fate Data Covance 6329-228 3M T-6316.1 PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92429 C92430 C92431 C92432 C92433 C92434 C92435 C92436 C92437 C92438 C92439 C92440 C92441 C92442 C92443 C92444 C92445 C92446 C92447 C92448 C92449 C92450 C92451 C92452 C92453 C92454 C92455 C92456 C92457 C92458 C92459 C92460 C92461 C92462 DOSE GROUP ----- 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- D T T M 3 D 1 M M 3 T D 3 M T T M M M M 3 M T T M M M T M M M M M M TYPE OF DEATH ----------UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH DATE OF DEATH -------06/10/99 04/04/00 04/04/00 09/24/99 04/07/99 12/29/99 04/28/98 01/31/00 03/19/99 04/07/99 04/04/00 10/17/99 04/07/99 07/25/99 04/04/00 04/04/00 01/31/00 11/05/99 01/03/00 08/02/99 04/07/99 12/15/99 04/04/00 04/04/00 02/28/00 10/23/99 03/23/00 04/04/00 11/15/99 07/19/99 02/28/00 02/13/00 06/04/99 02/21/00 DAY OF STUDY ------431 730 730 537 367 633 23 666 348 367 730 560 367 476 730 730 666 579 638 484 367 619 730 730 694 566 718 730 589 470 694 679 425 687 WEEK OF STUDY ------62 105 105 77 53 91 4 96 50 53 105 80 53 68 105 105 96 83 92 70 53 89 105 105 100 81 103 105 85 68 100 97 61 99 3782 Covance 6329-212 APPENDIX 2 Individual Animal Fate Data Covance 6329-228 3M T-6316.1 PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92463 C92464 C92465 C92466 C92467 C92468 C92469 C92470 DOSE GROUP ----- 9 9 9 9 9 9 9 9 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- 1 2 2 2 1 2 2 1 TYPE OF DEATH ----------SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------FIRST INTERIM SACRIFICE SECOND INTERIM SACRIFICE SECOND INTERIM SACRIFICE SECOND INTERIM SACRIFICE FIRST INTERIM SACRIFICE SECOND INTERIM SACRIFICE SECOND INTERIM SACRIFICE FIRST INTERIM SACRIFICE DATE OF DEATH -------04/28/98 07/07/98 07/07/98 07/07/98 04/28/98 07/07/98 07/07/98 04/28/98 DAY OF STUDY ------23 93 93 93 23 93 93 23 WEEK OF STUDY ------4 14 14 14 4 14 14 4 3783 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92191 M 104 APPEARANCE SWOLLEN PAW-HIND-LEFT 708 EYES PALE EYES 701, 708, 715, 722 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435 MASS(ES) INGUINAL REGION-LEFT WEEK 64 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C C92192 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 3784 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92193 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 701, 708, 715, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C92194 M 101 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 706 COLD TO TOUCH ENTIRE BODY 706 EXCRETION NO FECES 706 EYES DILATED PUPIL EYES 706 PALE EYES 706 RESPIRATION AUDIBLE 706 LABORED 706 3785 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92194 M 101 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701 C92195 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C92196 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 C92197 T 105 APPEARANCE MALOCCLUSION SWOLLEN PAW-HIND-LEFT THIN 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694 701, 708, 715, 722, 729-730 729-730 582, 708, 715, 722, 729-730 EXCRETION FEW FECES 498, 505 3786 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92197 T 105 SKIN & PELAGE SORE/SCAB DORSAL 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330 C92198 D 11 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 75 C92199 3 53 SKIN & PELAGE SORE/SCAB SCROTUM 337, 344, 351 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 358, 365, 367 C92200 M 77 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 526, 533-534 EXCRETION FEW FECES 526, 533-534 3787 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92200 M 77 EYES RED DISCHARGE EYE-LEFT 519, 526 EYES 533-534 SKIN & PELAGE SORE/SCAB TAIL-PROXIMAL 498, 505 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463 WART LIKE LESION(S) NOSE WEEK 68 TAIL-PROXIMAL WEEK 74 470, 477, 484, 491 512, 519, 526, 533-534 C92201 M 95 APPEARANCE LIMITED USE LIMBS-HIND 659, 662 EXCRETION FEW FECES 662 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 3788 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92201 M 95 NORMAL NO REMARKABLE OBSERVATIONS 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 C92202 D 86 APPEARANCE MALOCCLUSION SWOLLEN PAW-HIND-RIGHT THIN 592, 596, 599 491, 498, 505 589, 592, 596, 599 DISCHARGE OTHER 592C EXCRETION FEW FECES 582, 589, 592, 596, 599 RESPIRATION IRREGULAR 592 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 512, 519, 526, 533, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 540, 547, 554, 561, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 568, 575 3789 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92203 T 105 APPEARANCE OBESE SWOLLEN PAW-HIND-LEFT THIN 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 638, 645, 652, 659 693-694, 701, 708, 715, 722, 729-730 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 666, 673, 680, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 687 C92204 D 82 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 570 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C92205 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 3790 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92206 M 76 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 526-527 DISCHARGE NASAL RED IN COLOR 512, 519 EXCRETION FEW FECES 526-527 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C92207 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 C92208 T 105 APPEARANCE MALOCCLUSION 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323 EXCRETION NONFORMED FECES 708 SKIN & PELAGE SORE/SCAB DORSAL-THORACIC-RIGHT 722, 729-730 3791 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92208 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 715 C92209 T 105 APPEARANCE MALOCCLUSION THIN 71, 78, 85, 92, 99, 106, 113, 120, 127, 134, 141, 148, 155, 162 169, 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708 715, 722, 729-730 701, 708 EYES RED DISCHARGE EYES 64 RESPIRATION AUDIBLE 64 SKIN & PELAGE PALE ENTIRE BODY 701, 708, 715, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57 3792 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92210 M 99 APPEARANCE MALOCCLUSION 64, 71, 78, 85, 92, 162, 169, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 617, 624, 631, 638, 99, 106, 113, 120, 127, 134, 141, 148, 155 190, 197, 204, 211, 218, 225, 232, 239, 246 281, 288, 295, 302, 309, 316, 323, 330, 337 372, 379, 386, 393, 400, 407, 414, 421, 428 463, 470, 477, 484, 491, 498, 505, 512, 519 554, 561, 568, 575, 582, 589, 596, 603, 610 645, 652, 659, 666, 673, 680, 683, 687-689 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 688-689 DISCHARGE NASAL RED IN COLOR 688 EXCRETION FEW FECES NO FECES NONFORMED FECES 688 689 393, 652 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE PERINEAL AREA YELLOW HAIR COAT 683, 687-688 689 687-689 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57 3793 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92211 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C92212 M 104 APPEARANCE THIN 701, 708, 714-715, 718, 722 DISCHARGE NASAL RED IN COLOR 714-715, 718, 722 EXCRETION FEW FECES NO FECES NONFORMED FECES 714-715 718, 722 204 SKIN & PELAGE PALE ENTIRE BODY RED HAIR COAT PERINEAL AREA 722 715, 718, 722 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 211, 218, 302, 309, 393, 400, 484, 491, 575, 582, 22, 29, 36, 43, 50, 127, 134, 141, 148, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 589, 596, 603, 610, 57, 64, 71, 78, 85, 155, 162, 169, 176, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 617, 624, 631, 638, 92, 99, 106 183, 190, 197 281, 288, 295 372, 379, 386 463, 470, 477 554, 561, 568 645, 652, 659 3794 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92212 M 104 NORMAL NO REMARKABLE OBSERVATIONS 666, 673, 680, 687, 694 C92213 M 51 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 356 COLD TO TOUCH ENTIRE BODY 356 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 351, 356 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344 C92214 M 85 APPEARANCE THIN 589 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 589 DISCHARGE NASAL RED IN COLOR 589 EXCRETION NO FECES 589 3795 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92214 M 85 SKIN & PELAGE YELLOW HAIR COAT 589 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C92215 M 76 APPEARANCE MALOCCLUSION SWOLLEN NOSE 428, 435, 442, 449, 470, 477, 484, 491, 498, 505, 512, 519 526-527 470, 477, 484, 491, 498, 505, 512 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 526-527 EXCRETION FEW FECES 526-527 EYES SQUINTED PERI-ORBITAL-LEFT 470, 477, 484, 491, 498, 505, 512, 519, 526-527 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL 512, 519, 526-527 3796 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92215 M 76 SKIN & PELAGE BROWN HAIR COAT VENTRAL-CERVICAL 512, 519, 526-527 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 456, 463 C92216 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 C92217 M 79 SKIN & PELAGE SORE/SCAB PAW-FRONT-LEFT VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT 421 141, 442, 449, 456 197 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 148, 155, 162, 169, 176, 183, 204, 211, 218, 253 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 428 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 28 VENTRAL-ABDOMINAL-RIGHT WEEK 33 WEEK 63 190 225C, 232C, 239C, 246C 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C 3797 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92217 M 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 63 512C, 519C, 526C, 533C, 540C, 547C WART LIKE LESION(S) VENTRAL-ABDOMINAL WEEK 20 134 C92218 T 105 APPEARANCE MALOCCLUSION MISSING TEETH THIN 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113, 120, 127, 134, 141 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687 693-694, 701, 708, 715, 722, 729-730 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 693-694, 701, 708, 715, 722, 729-730 693-694, 701, 708, 715, 722, 729-730 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 386, 400, 442, 449, 456, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 693-694, 701, 708, 715, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36 3798 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92218 T 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 79 547, 554, 561 C92219 M 87 BEHAVIOR OTHER 607C EXCRETION FEW FECES 607 SKIN & PELAGE SORE/SCAB TAIL-DISTAL 607 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C92220 D 77 EYES RED DISCHARGE EYE-LEFT 498 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 449 3799 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92220 D 77 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 456, 463, 470, 477 484, 491, 505, 512, 519, 526, 533, 538 C92221 M 93 APPEARANCE HUNCHED THIN 645 582, 589, 596, 603, 610, 617, 624, 631, 638, 645 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 645 EXCRETION FEW FECES 645 EYES RED DISCHARGE EYES 645 SQUINTED PERI-ORBITAL 645 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 386, 393, 400, 407, 414, 421 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 3800 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92221 M 93 NORMAL NO REMARKABLE OBSERVATIONS 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 519, 526, 533, 540, 547, 554, 561, 568, 575 C92222 T 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 526, 533, 540 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 596, 603, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 610, 617, 624, 631, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 547, 554, 561, 568, 638, 645, 652 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 575, 582, 589 MASS(ES) DORSAL WEEK 95 VENTRAL-ABDOMINAL WEEK 75 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729-730C 519C 3801 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92223 3 53 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT 295, 302, 309, 316, 323 225, 232 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 239, 246 253, 260, 267, 330, 337, 344, 351, 358, 365, 367 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 40 VENTRAL-ABDOMINAL-RIGHT WEEK 28 274C, 281C, 288C 190, 197, 204, 211C, 218C C92224 D 103 APPEARANCE SWOLLEN PAWS-HIND 708, 715-716 EXCRETION NONFORMED FECES 498, 505 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL SORE/SCAB DORSAL-THORACIC-LEFT PAW-HIND-RIGHT PAWS-HIND VENTRAL-ABDOMINAL 715-716 624 582, 589, 596 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687 694, 697, 701, 708, 715-716 232, 239, 246, 547, 554, 561, 568, 575 3802 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 20 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92224 D 103 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 512, 519 526, 533 MASS(ES) VENTRAL-ABDOMINAL WEEK 32 WEEK 78 218C, 225C, 232C, 239C, 246C 540, 547, 554, 561 WART LIKE LESION(S) DORSAL WEEK 93 645, 652, 659, 666, 673, 680, 687, 694, 697, 701, 708, 715-716 C92225 M 71 APPEARANCE THIN 477, 484, 491, 494 BEHAVIOR ATAXIC <UNSPECIFIED> 494 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA ROUGH HAIR COAT 494 491, 494 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 3803 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 21 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92225 M 71 NORMAL NO REMARKABLE OBSERVATIONS 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 C92226 M 91 APPEARANCE THIN 624, 631 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 631 EYES RED DISCHARGE EYE-RIGHT 631 RESPIRATION IRREGULAR 631 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 3804 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 22 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92227 M 102 APPEARANCE MALOCCLUSION THIN 190 701, 708, 710-712 BEHAVIOR HYPOACTIVE <UNSPECIFIED> COLD TO TOUCH ENTIRE BODY 708, 710-712 711-712 EXCRETION FEW FECES NONFORMED FECES 673, 711-712 710 SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB VENTRAL-ABDOMINAL 708, 710-712 519, 526, 533, 540, 547, 554, 561, 568, 575 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 211, 218, 302, 309, 393, 400, 484, 491, 645, 652, 22, 29, 36, 43, 50, 127, 134, 141, 148, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 582, 589, 659, 666, 680, 687, 57, 64, 71, 78, 85, 155, 162, 169, 176, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 596, 603, 610, 617, 694 92, 99, 106 183, 197, 204 281, 288, 295 372, 379, 386 463, 470, 477 624, 631, 638 MASS(ES) VENTRAL-ABDOMINAL WEEK 74 512, 519 3805 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 23 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92228 M 64 APPEARANCE HUNCHED PROTRUDING PENIS THIN 442-443, 446 414 421, 428, 435, 442-443, 446 BEHAVIOR ATAXIC <UNSPECIFIED> 443, 446 EXCRETION FEW FECES 421, 428, 435, 442-443, 446 RESPIRATION LABORED 443, 446 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL 446 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407 3806 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 24 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92229 D 86 APPEARANCE MALOCCLUSION 211, 218, 225 EXCRETION FEW FECES NONFORMED FECES 407, 414, 421 512 SKIN & PELAGE SORE/SCAB DORSAL DORSAL-CERVICAL-RIGHT LUMBAR-RIGHT 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 600 463, 470, 477, 484, 491 183, 190, 197 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 204, 232, 239 246, 253, 260, 267, 274 WART LIKE LESION(S) DORSAL WEEK 41 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 C92230 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 3807 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 25 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92231 T 105 EXCRETION NONFORMED FECES 694, 701, 708, 715 SKIN & PELAGE SORE/SCAB VENTRAL-THORACIC-RIGHT 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 722 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C92232 M 100 APPEARANCE MALOCCLUSION 456, 463, 470 EXCRETION FEW FECES NO FECES 673, 680, 687 690, 694 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE ROUGH HAIR COAT 687, 690, 694 690, 694 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 3808 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 26 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92232 M 100 NORMAL NO REMARKABLE OBSERVATIONS 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 C92233 T 105 APPEARANCE THIN 701, 708, 715, 722, 729-730 EXCRETION FEW FECES NO FECES NONFORMED FECES 701, 708, 715, 722 106 134 EYES SQUINTED PERI-ORBITAL 701, 708, 715, 722, 729-730 SKIN & PELAGE YELLOW HAIR COAT 701, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 120, 127, 218, 225, 309, 316, 400, 407, 491, 498, 582, 589, 673, 680, 22, 29, 36, 43, 50, 141, 148, 155, 162, 232, 239, 246, 253, 323, 330, 337, 344, 414, 421, 428, 435, 505, 512, 519, 526, 596, 603, 610, 617, 687, 694 57, 64, 71, 78, 85, 169, 176, 183, 190, 260, 267, 274, 281, 351, 358, 365, 372, 442, 449, 456, 463, 533, 540, 547, 554, 624, 631, 638, 645, 92, 99, 113 197, 204, 211 288, 295, 302 379, 386, 393 470, 477, 484 561, 568, 575 652, 659, 666 3809 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92234 M 85 APPEARANCE OBESE THIN 547, 554, 561, 568, 575, 580, 582, 587 589 EXCRETION FEW FECES NO FECES 580, 582, 587 589 EYES SQUINTED PERI-ORBITAL 580, 582, 587, 589 HOUSING,FOOD,WATER & MAINT. ANIMAL PUT IN PLASTIC CAGE 587 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL SORE/SCAB PAW-HIND-LEFT 575, 580, 582, 587, 589 575, 580, 582, 587, 589 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 3810 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 28 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92235 M 97 APPEARANCE MALOCCLUSION SWOLLEN NOSE 281, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498 505, 512, 519, 526, 533, 540, 568, 575, 582, 589, 659, 666, 673 677 677 RESPIRATION AUDIBLE 677 LABORED 677 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA 677 RED SKIN TAIL 323 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 302, 309, 400, 407, 652 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 316, 330, 337, 344, 414, 547, 554, 561, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 351, 358, 365, 372, 603, 610, 617, 624, 92, 99, 106 183, 190, 197 274, 288, 295 379, 386, 393 631, 638, 645 3811 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 29 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92236 D 90 APPEARANCE THIN 622, 624 EXCRETION FEW FECES 622, 624 EYES PALE EYES 622, 624 RESPIRATION IRREGULAR 622, 624 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C92237 M 101 APPEARANCE THIN 701, 706 BEHAVIOR HYPOACTIVE <UNSPECIFIED> RECUMBENT LATERAL 701, 706 706 3812 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 30 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92237 M 101 DISCHARGE NASAL RED IN COLOR 701, 706 EXCRETION NO FECES 706 NONFORMED FECES 624 EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL 701, 706 701, 706 RESPIRATION LABORED 706 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687 694, 701, 706 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 3813 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 31 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92238 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C92239 T 105 APPEARANCE HEAD TILT RIGHT THIN 729-730 729-730 EXCRETION FEW FECES NONFORMED FECES 701, 708, 715, 722, 729-730 134 EYES SQUINTED PERI-ORBITAL 729-730 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL SORE/SCAB PAW-HIND-LEFT 701, 708, 715, 722, 729-730 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 211, 218, 302, 309, 393, 400, 484, 491, 22, 29, 36, 43, 50, 127, 141, 148, 155, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 57, 64, 71, 78, 85, 162, 169, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 92, 99, 106 190, 197, 204 281, 288, 295 372, 379, 386 463, 470, 477 554, 561, 568 3814 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 32 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92239 T 105 NORMAL NO REMARKABLE OBSERVATIONS 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659 666, 673, 680, 687, 694 C92240 D 83 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 533, 540, 547, 554, 561, 568, 575, 578 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 498, 505, 512, 519, 526 WART LIKE LESION(S) HEAD-MAXILLARY-RIGHT WEEK 64 442, 449, 456, 463, 470, 477, 484, 491 C92241 M 93 APPEARANCE HEAD TILT LEFT 644-645 EXCRETION FEW FECES NONFORMED FECES 644-645 498, 505 EYES RED DISCHARGE EYE-LEFT 644-645 3815 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 33 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92241 M 93 SKIN & PELAGE ROUGH HAIR COAT 644-645 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 582, 589, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 512, 519, 526, 596, 603, 610, 617, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 533, 540, 547, 554, 624, 631, 638 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 561, 568, 575 C92242 M 67 APPEARANCE HUNCHED 465 THIN 465 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 465 EXCRETION NO FECES 465 EYES SQUINTED PERI-ORBITAL 465 PERI-ORBITAL-LEFT 465 RESPIRATION IRREGULAR 465 3816 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 34 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92242 M 67 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA 465 RED HAIR COAT HEAD-ENTIRE 211, 218, 225 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 435, 442, 449, 456, 463 C92243 M 70 APPEARANCE HUNCHED 485 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 485 RESPIRATION LABORED 485 SKIN & PELAGE PALE ENTIRE BODY 485 RED HAIR COAT VENTRAL-ABDOMINAL 485 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 3817 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 35 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92243 M 70 NORMAL NO REMARKABLE OBSERVATIONS 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484 C92244 D 65 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 453 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372 C92245 M 68 APPEARANCE MALOCCLUSION THIN OTHER 365, 372, 379, 386, 393, 400, 414, 421, 428, 435, 441-442 448-449, 456, 463, 470, 472, 476 470, 472, 476 470C EXCRETION FEW FECES NONFORMED FECES 441-442, 448-449, 456, 463, 470, 472, 476 295, 441-442, 448 EYES PALE EYES 470 3818 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 36 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92245 M 68 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 449 YELLOW HAIR COAT 472, 476 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 302, 309, 316, 323, 330, 337, 344, 351, 358, 407 C92246 3 53 EXCRETION NONFORMED FECES 134, 176, 183, 344 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 141, 148, 155, 162, 169, 190, 197, 204, 211, 218 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309 316, 323, 330, 337, 351, 358, 365, 367 C92247 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 3819 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 37 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92248 M 69 APPEARANCE THIN 449, 456, 463, 470, 472, 477 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 477 EXCRETION FEW FECES 470, 472, 477 EYES SQUINTED PERI-ORBITAL 470, 472, 477 SKIN & PELAGE ROUGH HAIR COAT 449, 456, 463, 470, 472, 477 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442 C92249 D 72 EXCRETION NONFORMED FECES 295 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463 470, 477, 484, 491, 498-499 3820 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 38 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92249 D 72 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372 C92250 M 91 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 637 EYES RED DISCHARGE EYES 637 SKIN & PELAGE SORE/SCAB DORSAL-CERVICAL PAW-HIND-RIGHT 29, 36, 43, 50, 57, 64, 71, 78, 85 624, 631, 637 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 162, 169, 253, 260, 344, 351, 435, 442, 526, 533, 617 22, 92, 99, 106, 113, 120, 127, 134, 141, 148, 155 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 3821 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 39 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92251 M 84 RESPIRATION IRREGULAR 582 SKIN & PELAGE SORE/SCAB LATERAL-ABDOMINAL-RIGHT PAW-HIND-LEFT YELLOW HAIR COAT 576, 582 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533 540, 547, 554, 561, 568 582 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 78 540C, 547C, 554C, 561C, 568C, 575-576C, 582C C92252 T 105 APPEARANCE PROTRUDING PENIS THIN 708, 715, 722 729-730 EXCRETION NONFORMED FECES 708, 715, 722 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 3822 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 40 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92252 T 105 NORMAL NO REMARKABLE OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701 C92253 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C92254 M 87 APPEARANCE THIN 582-583, 587, 589, 596, 603 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 589 DISCHARGE GENITAL RED IN COLOR NASAL RED IN COLOR 386 589, 596, 603 EXCRETION FEW FECES NO FECES 582, 603 583, 587, 589, 596 3823 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 41 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92254 M 87 EYES RED DISCHARGE EYES 583, 587, 589, 596, 603 HOUSING,FOOD,WATER & MAINT. ANIMAL PUT IN PLASTIC CAGE 587 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 533, 540, 547, 554, 561, 568, 575, 582-583, 587, 589, 596, 603 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 393, 400, 484, 491, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 407, 414, 421, 428, 498, 505, 512, 519, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 435, 442, 449, 456, 526 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 463, 470, 477 C92255 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92256 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 3824 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 42 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92257 2 14 APPEARANCE MALOCCLUSION 71, 78, 85, 92-93 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64 C92258 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92259 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 C92260 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 3825 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 43 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92191 M8 442 64 TO 449 65 MASS(ES) INGUINAL REGION-LEFT WEEK 64 30 X 20MM 456 66 MASS(ES) INGUINAL REGION-LEFT WEEK 64 30 X 30MM 463 67 TO 505 73 MASS(ES) INGUINAL REGION-LEFT WEEK 64 40 X 35MM 512 74 MASS(ES) INGUINAL REGION-LEFT WEEK 64 50 X 55MM 519 75 TO 533 77 MASS(ES) INGUINAL REGION-LEFT WEEK 64 60 X 55MM 540 78 TO 554 80 MASS(ES) INGUINAL REGION-LEFT WEEK 64 70 X 55MM 561 81 MASS(ES) INGUINAL REGION-LEFT WEEK 64 70 X 60MM 568 82 TO 582 84 MASS(ES) INGUINAL REGION-LEFT WEEK 64 75 X 65MM 3826 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 44 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92191 M8 589 85 TO 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 64 75 X 70MM 603 87 MASS(ES) INGUINAL REGION-LEFT WEEK 64 75 X 75MM 610 88 TO 617 89 MASS(ES) INGUINAL REGION-LEFT WEEK 64 75 X 90MM 624 90 TO 638 92 MASS(ES) INGUINAL REGION-LEFT WEEK 64 80 X 90MM 645 93 TO 659 95 MASS(ES) INGUINAL REGION-LEFT WEEK 64 90 X 90MM 666 96 MASS(ES) INGUINAL REGION-LEFT WEEK 64 100 X 90MM 673 97 TO 694 100 MASS(ES) INGUINAL REGION-LEFT WEEK 64 110 X 90MM 701 101 MASS(ES) INGUINAL REGION-LEFT WEEK 64 130 X 100MM 3827 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 45 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92191 M8 708 102 MASS(ES) INGUINAL REGION-LEFT WEEK 64 50 X 50MM 715 103 TO 722 104 MASS(ES) INGUINAL REGION-LEFT WEEK 64 135 X 100MM C92202 M8 592 85 DISCHARGE OTHER RED DISCHARGE C92217 M8 225 33 TO 246 36 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 33 15 X 15MM 435 63 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 63 10 X 10MM 442 64 TO 456 66 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 63 50 X 40MM 463 67 TO 491 71 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 63 60 X 60MM 498 72 TO 505 73 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 63 70 X 70MM 3828 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 46 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92217 M8 512 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 63 80 X 80MM 519 75 TO 533 77 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 63 120 X 100MM 540 78 TO 547 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 63 140 X 100MM C92219 M8 607 87 TO 607 87 BEHAVIOR OTHER ANIMAL IS CHEWING ON DISTAL PORTION OF TAIL C92222 M8 729 105 TO 730 105 MASS(ES) DORSAL WEEK 95 15 X 10MM ANTERIOR 10 X 10MM POSTERIOR 519 75 MASS(ES) VENTRAL-ABDOMINAL WEEK 75 20 X 20MM C92223 M8 274 40 TO 288 42 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 40 15 X 15MM 211 31 TO 218 32 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 28 15 X 15MM 3829 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 47 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92224 M8 218 32 TO 246 36 MASS(ES) VENTRAL-ABDOMINAL WEEK 32 10 X 10MM C92245 M8 470 68 APPEARANCE OTHER EXCESSIVE BLEEDING FROM PAWS, CAUTERIZED WITH SILVER NITRATE C92251 M8 540 78 TO 547 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 78 35 X 35MM 554 80 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 78 40 X 35MM 561 81 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 78 80 X 60MM 3830 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 48 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92261 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 582, 589, 673, 680, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 596, 603, 610, 617, 687, 694, 701, 708, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 540, 547, 554, 624, 631, 638, 645, 715, 722, 729-730 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 561, 568, 575 652, 659, 666 MASS(ES) VENTRAL-ABDOMINAL WEEK 76 526, 533 C92262 M 104 APPEARANCE THIN 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 SKIN & PELAGE ALOPECIA PALE ENTIRE BODY SORE/SCAB DORSAL HEAD-ENTIRE VENTRAL-CERVICAL-RIGHT 708, 715, 722 708, 715, 722 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 610, 617, 624, 631 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 3831 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 49 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92262 M 104 NORMAL NO REMARKABLE OBSERVATIONS 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603 C92263 T 105 APPEARANCE MALOCCLUSION 337, 344, 351, 358, 365, 372, 638, 645, 652, 659, 666, 673 DISCHARGE GENITAL RED IN COLOR 701 EXCRETION NONFORMED FECES 722, 729-730 SKIN & PELAGE RED HAIR COAT DORSAL SORE/SCAB PAW-HIND-LEFT 694, 701, 708, 715, 722 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 708, 715, 722 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 379, 386, 393, 400, 407, 414, 421 428, 435 WART LIKE LESION(S) TAIL-PROXIMAL WEEK 64 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617 3832 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 50 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92263 T 105 WART LIKE LESION(S) TAIL-PROXIMAL WEEK 64 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708 715, 722, 729-730 C92264 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 C92265 D 80 APPEARANCE CONVULSIONS MALOCCLUSION MISSING TEETH 540 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106, 113, 120, 127, 134, 141, 148 183, 190, 197, 204, 211, 218, 225, 232, 239 274, 281, 288, 295, 302, 309, 316, 323, 330 365, 372, 379, 386, 393, 400, 407, 414, 421 456, 463, 470, 477, 484, 491, 498, 505, 512 547, 554 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547, 554 DISCHARGE CLEAR ORAL <UNSPECIFIED> MODERATE 540 253, 260 EXCRETION NONFORMED FECES 134, 204, 498, 505 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 3833 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 51 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92266 T 105 DISCHARGE GENITAL RED IN COLOR 141 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 519 WART LIKE LESION(S) DORSAL WEEK 62 WEEK 76 VENTRAL-ABDOMINAL WEEK 92 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 722, 729-730 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729-730 C92267 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 3834 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 52 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92268 M 100 APPEARANCE OBESE THIN 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603 610, 617, 624, 631 694 EXCRETION NONFORMED FECES 204 SKIN & PELAGE SORE/SCAB DORSAL-THORACIC-LEFT SCROTUM-RIGHT 638, 645, 652, 659 666, 673, 680, 687, 694 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 211, 218, 302, 309, 393, 400, 484, 491 22, 29, 36, 43, 50, 127, 134, 141, 148, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 57, 64, 71, 78, 85, 155, 162, 169, 176, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 92, 99, 106 183, 190, 197 281, 288, 295 372, 379, 386 463, 470, 477 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 72 498, 505, 512 3835 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92269 D 73 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505-506 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C92270 T 105 EXCRETION NONFORMED FECES 624, 631 SKIN & PELAGE BLACK SKIN VENTRAL-ABDOMINAL SORE/SCAB VENTRAL-ABDOMINAL 694 295, 302, 442, 449, 701 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 330, 337, 421, 428, 526, 533, 617, 638, 729-730 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 344, 351, 358, 365, 435, 456, 463, 470, 540, 547, 554, 561, 645, 652, 659, 666, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 372, 379, 386, 393, 477, 484, 491, 498, 568, 575, 582, 589, 673, 680, 687, 708, 92, 99, 106 183, 190, 197 309, 316, 323 400, 407, 414 505, 512, 519 596, 603, 610 715, 722 MASS(ES) VENTRAL-ABDOMINAL WEEK 40 274C, 281C, 288C 3836 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 54 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92271 D 90 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) DORSAL WEEK 90 624-625C C92272 M 85 SKIN & PELAGE SORE/SCAB HEAD-MAXILLARY-RIGHT PAW-HIND-RIGHT PAWS-HIND 519, 526, 533, 540, 547, 554, 561, 568, 575, 582-583, 589-590 582-583, 589-590 568, 575 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 74 512, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 582-583C, 589-590C 3837 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 55 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92273 D 42 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 294 C92274 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C92275 M 104 APPEARANCE THIN 701, 708, 715, 722 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 722 EYES SQUINTED PERI-ORBITAL 722 SKIN & PELAGE PALE ENTIRE BODY 701, 708, 715, 722 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 3838 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 56 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92275 M 104 NORMAL NO REMARKABLE OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 582, 589, 596, 603 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694 MASS(ES) INGUINAL REGION-LEFT WEEK 78 540C, 547C, 554C, 561C, 568C, 575C C92276 D 73 APPEARANCE THIN 498, 505, 510 SKIN & PELAGE RED HAIR COAT DORSAL-CERVICAL-RIGHT 505, 510 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C92277 M 60 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 415 RECUMBENT STERNAL 415 EXCRETION NO FECES 414-415 3839 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 57 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92277 M 60 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL 414-415 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407 C92278 T 105 EXCRETION NONFORMED FECES 288, 295, 463, 470, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 687, 694, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 309, 316, 400, 407, 505, 512, 715 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 323, 330, 337, 344, 414, 421, 428, 435, 519, 526, 533, 540, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 351, 358, 365, 372, 442, 449, 456, 477, 547, 554, 561, 568, 92, 99, 106 183, 190, 197 274, 281, 302 379, 386, 393 484, 491, 498 575, 701, 708 C92279 M 89 APPEARANCE HEAD TILT RIGHT HUNCHED SWOLLEN PAW-HIND-RIGHT 614, 617 614, 617 568, 575, 582, 589, 596, 603 3840 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 58 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92279 M 89 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 610, 614, 617 DISCHARGE GENITAL RED IN COLOR 316, 603, 614 EXCRETION FEW FECES 610, 614, 617 EYES RED DISCHARGE EYE-LEFT 155 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT 337, 344, 351 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 211, 218, 302, 309, 435, 442, 526, 533, 22, 29, 36, 43, 50, 127, 134, 141, 148, 225, 232, 239, 246, 358, 365, 372, 379, 449, 456, 463, 470, 540, 547, 554, 561 57, 64, 71, 78, 85, 162, 169, 176, 183, 253, 260, 267, 274, 386, 393, 400, 407, 477, 484, 491, 498, 92, 99, 106 190, 197, 204 281, 288, 295 414, 421, 428 505, 512, 519 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 316C, 323C, 330C 3841 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 59 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92280 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 C92281 T 105 EXCRETION NONFORMED FECES 652 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 414, 421, 505, 512, 596, 603, 694, 701, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 127, 134, 141, 148, 155, 162, 169, 176, 218, 225, 232, 239, 246, 253, 260, 267, 337, 344, 351, 358, 365, 372, 379, 386, 428, 435, 442, 449, 456, 463, 470, 477, 519, 526, 533, 540, 547, 554, 561, 568, 610, 617, 624, 631, 638, 645, 659, 666, 708, 715, 722, 729-730 92, 99, 106 183, 190, 197 274, 281, 288 393, 400, 407 484, 491, 498 575, 582, 589 673, 680, 687 MASS(ES) VENTRAL-ABDOMINAL WEEK 45 309C, 316C, 323C, 330C C92282 T 105 EXCRETION NONFORMED FECES 288, 295, 463, 470, 729-730 SKIN & PELAGE YELLOW HAIR COAT 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694, 701, 708, 715, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 302 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 3842 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 60 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92282 T 105 NORMAL NO REMARKABLE OBSERVATIONS 400, 407, 414, 421, 428, 435, 442, 449, 456, 477, 484, 491, 498 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 WART LIKE LESION(S) LIMB-FRONT-RIGHT WEEK 105 729-730 C92283 T 105 EXCRETION NONFORMED FECES 638 SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL-LEFT RED HAIR COAT DORSAL-CERVICAL SORE/SCAB VENTRAL-ABDOMINAL 694 442, 449, 456, 463 701, 708 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 505, 512, 596, 603, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 519, 526, 533, 540, 610, 617, 624, 631, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 470, 477, 547, 554, 561, 568, 645, 652, 659, 666, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 484, 491, 498 575, 582, 589 673, 680, 687 WART LIKE LESION(S) TAIL-MID WEEK 101 701, 708, 715, 722, 729-730 3843 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 61 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92284 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 C92285 M 76 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 519, 526-527 EXCRETION FEW FECES 526-527 SKIN & PELAGE ROUGH HAIR COAT 519, 526-527 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C92286 3 53 SKIN & PELAGE SORE/SCAB MOUTH 8, 15, 22 NORMAL NO REMARKABLE OBSERVATIONS 1, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113, 120 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 3844 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 62 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92287 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C92288 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C92289 T 105 APPEARANCE MALOCCLUSION THIN 64 701, 708, 715, 722, 729-730 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA VENTRAL-ABDOMINAL SORE/SCAB DORSAL 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729-730 386, 393 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 575, 582, 589, 596, 603, 610, 617 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 71, 78, 85, 92, 99, 106, 113 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 400 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 624, 631 3845 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 63 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92289 T 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 79 547, 554, 561, 568, 575, 582, 589, 596, 603 C92290 M 63 APPEARANCE HUNCHED THIN 421, 428, 431, 435, 438 421, 428, 431, 435, 438 BEHAVIOR ATAXIC <UNSPECIFIED> 438 HYPOACTIVE <UNSPECIFIED> 438 EXCRETION FEW FECES NO FECES 414, 421, 428, 431, 435 438 RESPIRATION IRREGULAR 438 SKIN & PELAGE ROUGH HAIR COAT 428, 431, 435, 438 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407 3846 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 64 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92291 D 97 EXCRETION NONFORMED FECES 302, 309 EYES RED DISCHARGE EYE-LEFT 442 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 316, 400, 407, 498, 505, 589, 596, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 323, 330, 337, 344, 414, 421, 428, 435, 512, 519, 526, 533, 603, 610, 617, 624, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 351, 358, 365, 372, 449, 456, 463, 470, 540, 547, 554, 561, 631, 638, 645, 652, 92, 99, 106 183, 190, 197 274, 281, 288 379, 386, 393 477, 484, 491 568, 575, 582 659, 666, 673 C92292 M 67 APPEARANCE HUNCHED THIN 442, 449, 456, 462-463 421, 428, 435, 442, 449, 456, 462-463 BEHAVIOR SENSITIVE TO TOUCH 462-463 EXCRETION FEW FECES 462-463 EYES SQUINTED PERI-ORBITAL 462-463 RESPIRATION IRREGULAR 462-463 3847 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 65 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92292 M 67 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 442, 449, 456, 462-463 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414 C92293 D 42 EXCRETION NONFORMED FECES 260 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 267, 274, 281, 288 C92294 M 97 APPEARANCE THIN 638, 645, 652, 659, 666, 668, 673 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 673 COLD TO TOUCH ENTIRE BODY 673 EXCRETION NO FECES 668, 673 3848 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 66 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92294 M 97 SKIN & PELAGE PALE ENTIRE BODY 666, 668, 673 SORE/SCAB PAW-HIND-LEFT 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 668, 673 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C92295 T 105 EYES OPAQUE EYES PALE EYE-RIGHT 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729-730 638, 645, 652 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 3849 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 67 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92296 M 75 APPEARANCE THIN 519, 525 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 525 EXCRETION FEW FECES 519 NO FECES 525 EYES RED DISCHARGE EYES 525 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL SORE/SCAB VENTRAL-THORACIC 519, 525 498, 505 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 463, 470, 477, 484, 491, 512 WART LIKE LESION(S) VENTRAL-ABDOMINAL WEEK 58 400, 407, 414, 421, 428, 435, 442, 449, 456 3850 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 68 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92297 T 105 EYES SCAB EYE-LEFT SQUINTED PERI-ORBITAL 701, 708 638, 645, 652 SKIN & PELAGE SORE/SCAB LIMB-HIND-LEFT 715, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 680, 687, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 694 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 659, 666, 673 C92298 M 68 APPEARANCE HUNCHED MALOCCLUSION THIN 449, 456, 462-463, 470 442, 449, 456, 462-463, 470 449, 456, 462-463, 470 BEHAVIOR SENSITIVE TO TOUCH 462-463 EXCRETION FEW FECES NO FECES 442, 449, 456 462-463, 470 3851 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 69 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92298 M 68 SKIN & PELAGE YELLOW HAIR COAT 470 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435 C92299 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND 694, 697, 701, 708 694, 697, 701, 708 715, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 3852 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 70 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92300 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 C92301 D 87 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 605 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C92302 M 96 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 668 RECUMBENT STERNAL 668 DISCHARGE NASAL RED IN COLOR 666, 668 EXCRETION FEW FECES 666 NO FECES 668 EYES SQUINTED PERI-ORBITAL 668 RESPIRATION LABORED 668 3853 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 71 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92302 M 96 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA 666, 668 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 589, 596, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 603, 610, 617, 624, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 547, 554, 561, 631, 638, 645, 652, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 568, 575, 582 659 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 526, 533, 540 C92303 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C92304 M 96 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638 3854 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 72 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92304 M 96 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 93 645C, 652C, 659C, 666C C92305 M 63 APPEARANCE HUNCHED THIN 438 421, 438 BEHAVIOR ATAXIC <UNSPECIFIED> 438 HYPOACTIVE <UNSPECIFIED> 438 EXCRETION FEW FECES 438 NO FECES 106 RESPIRATION LABORED 438 SKIN & PELAGE SORE/SCAB DORSAL-CERVICAL HEAD-ENTIRE VENTRAL-CERVICAL 414, 421, 428, 435, 438 428, 435, 438 400, 407, 414, 421, 428, 435, 438 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 113 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 3855 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 73 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92305 M 63 NORMAL NO REMARKABLE OBSERVATIONS 393 C92306 D 104 EXCRETION NONFORMED FECES 638, 645, 652 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 680, 687, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 694, 701, 708, 715, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 722 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 659, 666, 673 C92307 M 101 APPEARANCE THIN 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 706-707 BEHAVIOR AGGRESSIVE <UNSPECIFIED> MODERATE SENSITIVE TO TOUCH 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 288, 295, 302, 309, 316, 323, 330 141, 148, 155, 162, 169, 176, 183, 190 134 RESPIRATION AUDIBLE IRREGULAR 701, 706-707 701, 706-707 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 3856 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 74 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92307 M 101 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 100 694, 701, 706-707 WART LIKE LESION(S) DORSAL WEEK 59 MOUTH WEEK 100 NOSE WEEK 100 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 706-707 694 694, 701, 706-707 C92308 T 105 APPEARANCE PARALYSIS LIMB-FRONT-LEFT THIN 50 729-730 SKIN & PELAGE SORE/SCAB DORSAL-CERVICAL YELLOW HAIR COAT 29, 36, 43 155 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 57, 64, 71, 78, 85, 92, 99, 106, 113, 120, 127 134, 141, 148, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316 3857 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 75 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92308 T 105 NORMAL NO REMARKABLE OBSERVATIONS 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708, 715, 722 C92309 T 105 APPEARANCE THIN 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C92310 D 73 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 507 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 3858 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 76 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92311 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729-730 C92312 M 65 APPEARANCE HUNCHED MALOCCLUSION PROTRUDING PENIS THIN 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 431, 435, 442, 449 407, 414, 421, 428, 431, 435, 442, 449 431 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 428, 431, 435, 442, 449 EXCRETION FEW FECES 449 EYES RED DISCHARGE EYE-LEFT EYE-RIGHT EYES 246, 253 267, 274, 281, 288, 295, 302, 309, 344, 351, 358, 365, 372, 379 386, 393, 400 316, 323, 330, 337 SKIN & PELAGE ROUGH HAIR COAT SORE/SCAB PAW-HIND-LEFT 414, 421, 428, 431, 435, 442, 449 379, 386, 393, 400, 407, 414, 421, 428, 431, 435, 442, 449 3859 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 77 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92312 M 65 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 260 C92313 D 97 EXCRETION NONFORMED FECES 204, 211, 512, 547, 554, 561 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 218, 225, 309, 316, 400, 407, 491 22, 29, 36, 43, 50, 127, 134, 141, 148, 232, 239, 246, 253, 323, 330, 337, 344, 414, 421, 428, 435, 57, 64, 71, 78, 85, 155, 162, 169, 176, 260, 267, 274, 281, 351, 358, 365, 372, 442, 449, 456, 463, 92, 99, 106 183, 190, 197 288, 295, 302 379, 386, 393 470, 477, 484 MASS(ES) DORSAL WEEK 72 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652C, 659C, 666C, 673-674C WART LIKE LESION(S) DORSAL WEEK 72 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 3860 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 78 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92314 M 93 APPEARANCE MALOCCLUSION THIN 643, 645, 649 638, 643, 645, 649 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 649 EXCRETION FEW FECES 638, 643, 645, 649 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB DORSAL 643, 645, 649 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 643, 645, 649 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 3861 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 79 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92315 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C92316 M 101 APPEARANCE HUNCHED 701-702 EYES PALE EYES 694, 701-702 SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 610, 702 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694 701-702 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 48 330, 337, 344, 351, 358, 365, 372, 379 3862 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 80 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92316 M 101 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 84 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C, 680C, 687C, 694C, 701-702C WART LIKE LESION(S) DORSAL WEEK 82 568 C92317 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C92318 T 105 EXCRETION NONFORMED FECES 498, 505, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 582, 589, 673, 680, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 512, 519, 526, 596, 603, 610, 617, 687, 694, 701, 708, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 533, 540, 547, 554, 624, 631, 638, 645, 715 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 561, 568, 575 652, 659, 666 3863 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 81 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92319 T 105 APPEARANCE MALOCCLUSION 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729-730 EXCRETION FEW FECES NONFORMED FECES 414, 421, 428, 435, 442, 449, 456, 463 498, 505 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 82 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659 666, 673, 680, 687, 694, 701, 708, 715, 722, 729-730C WART LIKE LESION(S) VENTRAL-THORACIC-RIGHT WEEK 82 568 3864 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 82 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92320 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C92321 T 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 MASS(ES) VENTRAL-ABDOMINAL WEEK 105 722C C92322 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 638, 645, 652, 659, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 666, 673, 680, 687, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 694, 701, 708 3865 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 83 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92322 T 105 NORMAL NO REMARKABLE OBSERVATIONS 715, 722, 729-730 MASS(ES) VENTRAL-ABDOMINAL WEEK 84 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C C92323 T 105 APPEARANCE LIMITED USE LIMB-HIND-LEFT THIN 724, 729-730 724 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL VENTRAL-THORACIC-RIGHT 575 708, 715 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 722 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 76 WEEK 84 526, 533, 540, 547, 554, 561, 568 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C, 680C, 687C, 694C 3866 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 84 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92323 T 105 WART LIKE LESION(S) VENTRAL-ABDOMINAL-RIGHT WEEK 82 568 VENTRAL-THORACIC-RIGHT WEEK 101 701 C92324 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C92325 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92326 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92327 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92328 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92329 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 3867 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 85 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92330 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 3868 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 86 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92270 M9 274 40 TO 288 42 MASS(ES) VENTRAL-ABDOMINAL WEEK 40 20 X 30MM C92271 M9 624 90 TO 625 90 MASS(ES) DORSAL WEEK 90 10 X 10MM C92272 M9 519 75 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 74 10 X 10MM 526 76 TO 561 81 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 74 20 X 15MM 568 82 TO 575 83 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 74 30 X 30MM 582 84 TO 590 85 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 74 30 X 40MM C92275 M9 540 78 TO 575 83 MASS(ES) INGUINAL REGION-LEFT WEEK 78 10 X 10MM C92279 M9 316 46 TO 330 48 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 10 X 10MM 3869 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 87 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92281 M9 309 45 TO 330 48 MASS(ES) VENTRAL-ABDOMINAL WEEK 45 10 X 10MM C92304 M9 645 93 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 93 20 X 30MM 652 94 TO 659 95 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 93 30 X 30MM 666 96 TO 666 96 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 93 35 X 30MM 666 96 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 93 30 X 30MM C92313 M9 575 83 MASS(ES) DORSAL WEEK 72 20 X 20MM 582 84 TO 631 91 MASS(ES) DORSAL WEEK 72 30 X 20MM 638 92 TO 659 95 MASS(ES) DORSAL WEEK 72 40 X 30MM 3870 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 88 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92313 M9 666 96 TO 674 97 MASS(ES) DORSAL WEEK 72 35 X 40MM C92316 M9 582 84 TO 617 89 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 84 20 X 20MM 624 90 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 84 25 X 25MM 666 96 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 84 30 X 30MM 673 97 TO 702 101 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 84 30 X 40MM C92319 M9 729 105 TO 730 105 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 82 10 X 10MM C92321 M9 722 104 MASS(ES) VENTRAL-ABDOMINAL WEEK 105 10 X 10MM C92322 M9 582 84 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL WEEK 84 15 X 20MM 3871 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 89 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92323 M9 582 84 TO 694 100 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 84 10 X 10MM 3872 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 90 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92331 M 92 APPEARANCE HUNCHED THIN 638, 641 638, 641 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 641 COLD TO TOUCH ENTIRE BODY 641 EXCRETION FEW FECES 638 NO FECES 641 EYES SQUINTED PERI-ORBITAL 641 RESPIRATION IRREGULAR 638, 641 SKIN & PELAGE YELLOW HAIR COAT 638, 641 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 288C, 295C, 302C, 309C, 316C, 323C, 330C, 337C, 344C, 351C, 358C 3873 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 91 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92331 M 92 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 638C, 641C C92332 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 C92333 T 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 393, 400 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 407, 414, 498, 505, 589, 596, 680, 687, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 421, 428, 435, 442, 512, 519, 526, 533, 603, 610, 617, 624, 694, 701, 708, 715, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 449, 456, 463, 470, 540, 547, 554, 561, 631, 638, 645, 652, 722, 729-730 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 477, 484, 491 568, 575, 582 659, 666, 673 MASS(ES) INGUINAL REGION-RIGHT WEEK 56 386, 393, 400 3874 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 92 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92334 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729-730 C92335 M 8 APPEARANCE SWOLLEN MOUTH 54 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C92336 M 82 SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT AXILLARY REGION-RIGHT 554, 561, 568, 570 568, 570 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400 MASS(ES) AXILLARY REGION-LEFT WEEK 59 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C 3875 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 93 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92336 M 82 MASS(ES) AXILLARY REGION-LEFT WEEK 59 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C 561C, 568C, 570C AXILLARY REGION-RIGHT WEEK 75 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 570C C92337 M 82 APPEARANCE HEAD TILT LEFT 569 DISCHARGE OTHER 274C EYES RED DISCHARGE EYE-LEFT EYE-RIGHT 554, 561, 568-569 512, 519 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 519 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 281, 288, 295 302, 309, 316, 323, 330, 337 MASS(ES) INGUINAL REGION-RIGHT WEEK 76 526, 533, 540, 547, 554, 561, 568-569 3876 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 94 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92337 M 82 MASS(ES) VENTRAL-ABDOMINAL WEEK 50 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C 568-569C VENTRAL-ABDOMINAL-LEFT WEEK 73 505C, 512C C92338 M 70 BEHAVIOR ATAXIC <UNSPECIFIED> 484 HYPOACTIVE <UNSPECIFIED> 484 EXCRETION FEW FECES 483-484 SKIN & PELAGE ALOPECIA RED HAIR COAT VENTRAL-ABDOMINAL VENTRAL-CERVICAL 344 483-484 483-484 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 3877 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 95 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92339 D 94 EYES RED DISCHARGE EYE-RIGHT 260, 267, 274, 379, 386, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 365, 372, 393, 400, 407 414 MASS(ES) SHOULDER-LEFT WEEK 38 VENTRAL-ABDOMINAL-RIGHT WEEK 66 260C, 267C, 274C, 281C, 288C, 295C, 302C, 309C, 316C, 323C, 330C 337C, 344C, 351C, 358C 456, 463, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C 610C, 617C, 624C, 631C, 638C, 645C, 652C C92340 M 79 SKIN & PELAGE BLACK SKIN VENTRAL-ABDOMINAL VENTRAL-THORACIC-LEFT 512, 519, 526, 533, 540, 547-548 512, 519, 526, 533, 540, 547-548 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295 3878 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 96 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92340 M 79 MASS(ES) INGUINAL REGION-LEFT WEEK 79 547-548C VENTRAL-ABDOMINAL WEEK 74 512C, 519C, 526C, 533C, 540C, 547-548C VENTRAL-ABDOMINAL-LEFT WEEK 44 302, 309, 316, 323, 330, 337, 344C, 351C, 358C, 365C, 372C, 379C 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C 540C, 547-548C VENTRAL-THORACIC-LEFT WEEK 74 512C, 519C, 526C, 533C, 540C, 547-548C C92341 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 365C, 367C C92342 M 50 APPEARANCE MALOCCLUSION THIN 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344 344 DISCHARGE NASAL RED IN COLOR 120 3879 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 97 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92342 M 50 EXCRETION FEW FECES 344 EYES RED DISCHARGE EYE-LEFT 120, 127, 134, 141 RESPIRATION AUDIBLE 344 SKIN & PELAGE YELLOW HAIR COAT 120, 344 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113 MASS(ES) AXILLARY REGION-RIGHT WEEK 40 274, 281, 288, 295, 302, 309, 316, 323, 330, 337 C92343 M 58 SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB SHOULDER-RIGHT 351, 358 365, 372, 379, 386, 393, 400 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120 3880 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 98 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92343 M 58 MASS(ES) SHOULDER-RIGHT WEEK 19 127C, 134C, 141C, 148C, 155C, 162C, 169C, 176C, 183C, 190C, 197C 204C, 211C, 218C, 225C, 232C, 239C, 246C, 253C, 260C, 267C, 274C 281C, 288C, 295C, 302C, 309C, 316C, 323C, 330C, 337C, 344C, 351C 358C, 365C, 372C, 379C, 386C, 393C, 400C C92344 M 104 APPEARANCE HUNCHED MALOCCLUSION 721-722 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 721-722 BEHAVIOR ATAXIC <UNSPECIFIED> 721-722 EXCRETION FEW FECES 582, 589, 596, 603 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 610, 617, 624, 631 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 3881 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 99 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92345 D 30 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 206 C92346 M 63 APPEARANCE HUNCHED THIN 428, 435 435 BEHAVIOR ATAXIC <UNSPECIFIED> 435 EXCRETION FEW FECES 393, 400, 407 EYES RED DISCHARGE EYES 435 SKIN & PELAGE ROUGH HAIR COAT YELLOW HAIR COAT 428, 435 393, 400, 407 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309 3882 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 100 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92346 M 63 WART LIKE LESION(S) NOSE WEEK 46 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 435 C92347 M 48 APPEARANCE THIN 330 RESPIRATION LABORED 330 SKIN & PELAGE YELLOW HAIR COAT 323, 330 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316 C92348 T 105 APPEARANCE MALOCCLUSION 106, 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 197, 204, 211, 218, 225, 232, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729-730 EXCRETION NONFORMED FECES 414 3883 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 101 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92348 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 701, 708, 715, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 239 MASS(ES) AXILLARY REGION-RIGHT WEEK 101 VENTRAL-ABDOMINAL WEEK 100 701C, 708C, 715C, 722C, 729-730C 694C, 701C, 708C, 715C, 722C, 729-730C C92349 D 78 APPEARANCE MALOCCLUSION 512, 519, 526, 533, 540-541 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 407, 414, 421, 428, 435, 442 449, 456, 463, 470, 477, 484, 491, 498, 505 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 50 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C, 400C 3884 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 102 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92350 M 94 APPEARANCE HEAD TILT RIGHT 656 DISCHARGE NASAL RED IN COLOR OTHER 656 656C EYES CLEAR DISCHARGE EYE-LEFT RED DISCHARGE EYES 638, 645, 652 655-656 RESPIRATION AUDIBLE 656 LABORED 656 SKIN & PELAGE PALE ENTIRE BODY 655-656 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 3885 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 103 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92351 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729-730 C92352 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 C92353 M 96 APPEARANCE HEAD TILT LEFT MALOCCLUSION 666-667 659 BEHAVIOR ATAXIC MODERATE HYPOACTIVE <UNSPECIFIED> 666-667 638, 645, 652, 658-659, 666-667 DISCHARGE NASAL RED IN COLOR 658-659, 666-667 EXCRETION FEW FECES NO FECES 638, 645, 652, 666-667 658-659, 666 3886 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 104 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92353 M 96 EYES RED DISCHARGE EYES 658 SQUINTED PERI-ORBITAL 658 RESPIRATION IRREGULAR 638 LABORED 667 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL WARM TO TOUCH ENTIRE BODY 658-659, 666-667 667 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 3887 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92354 T 105 APPEARANCE HEAD TILT LEFT 724, 729-730 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 724, 729-730 EXCRETION FEW FECES 724 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB AXILLARY REGION-RIGHT 701, 708, 715, 722, 724, 729-730 701, 708, 715, 722, 724, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) AXILLARY REGION-RIGHT WEEK 83 575, 582, 589, 596, 603, 610C, 617C, 624C, 631C, 638C, 645C 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 724C 3888 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 106 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92355 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 C92356 3 53 APPEARANCE MALOCCLUSION 57, 64, 71, 78, 85, 92, 99, 106, 113, 120, 127, 134, 141, 148 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232, 239 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330 337, 344, 351, 358, 365, 367 DISCHARGE NASAL RED IN COLOR 64 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C92357 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C92358 T 105 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547, 554, 561, 568, 575, 701, 708, 715, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 3889 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 107 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92358 T 105 NORMAL NO REMARKABLE OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 582, 589, 596, 603, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 729-730C C92359 D 93 APPEARANCE THIN 638, 644-645, 648 EXCRETION FEW FECES 638, 644-645, 648 EYES RED DISCHARGE EYES 644-645, 648 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) AXILLARY REGION-LEFT WEEK 92 AXILLARY REGION-RIGHT WEEK 79 638C, 644-645C, 648C 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C 3890 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 108 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92359 D 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 79 624C, 631C, 638C, 644-645C, 648C VENTRAL-ABDOMINAL WEEK 80 554C, 561C, 568C, 575C C92360 T 105 APPEARANCE MALOCCLUSION 701 SWOLLEN HEAD-MAXILLARY-RIGHT 701 SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL-LEFT BLACK SKIN VENTRAL-ABDOMINAL SORE/SCAB MOUTH PAWS-HIND 701, 708, 715, 722, 729-730 428, 435, 442, 449, 456 701, 708 701, 708, 715, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414 MASS(ES) AXILLARY REGION-LEFT WEEK 77 WEEK 96 533, 540, 547, 554, 561, 568, 575, 582C, 589C, 596C, 603C, 610C 617C, 624C, 631C 666, 673, 680, 687C, 694C, 701C, 708C, 715C, 722C, 729-730C 3891 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 109 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92360 T 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 92 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C 715C, 722C, 729-730C VENTRAL-ABDOMINAL-LEFT WEEK 61 421, 428, 435, 442, 449, 456, 463C, 470C, 477C, 484C, 491C, 498C 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 729-730C C92361 M 101 EYES PALE EYES 701-702 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) AXILLARY REGION-LEFT WEEK 80 VENTRAL-ABDOMINAL-LEFT WEEK 73 554, 561, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C 701-702C 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C, 680C, 687C, 694C, 701-702C 3892 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 110 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92362 T 105 SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL SORE/SCAB MOUTH 701, 708 554, 561, 568, 575 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 519, 526, 638, 645, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 533, 540, 547, 582, 652, 659, 666, 673, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 589, 596, 603, 610, 680, 687, 694 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 617, 624, 631 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 69 WEEK 102 477, 484, 491, 498, 505, 512 708C, 715C, 722C, 729-730C C92363 M 83 APPEARANCE MALOCCLUSION 260, 267, 274, 281, 344, 351, 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 SKIN & PELAGE SORE/SCAB HEAD-MAXILLARY-LEFT 344 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 288, 295, 302, 309, 316 3893 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 111 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92363 M 83 NORMAL NO REMARKABLE OBSERVATIONS 323, 330, 337, 484, 491, 498, 505, 512, 519 MASS(ES) AXILLARY REGION-LEFT WEEK 76 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C WART LIKE LESION(S) MOUTH WEEK 54 372, 379, 386 C92364 M 63 APPEARANCE HUNCHED THIN 428, 431, 435, 438 400, 407, 414, 421, 428, 431, 435, 438 BEHAVIOR ATAXIC <UNSPECIFIED> CIRCLING RIGHT 428, 431, 435, 438 438 EXCRETION FEW FECES 414, 421 EYES RED DISCHARGE EYE-LEFT 421, 428, 431, 435, 438 SKIN & PELAGE ROUGH HAIR COAT 428, 431, 435, 438 3894 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 112 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92364 M 63 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386 MASS(ES) AXILLARY REGION-RIGHT WEEK 57 393, 400, 407, 414, 421 C92365 3 53 APPEARANCE MALOCCLUSION 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 344, 351, 358, 365 367 C92366 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 C92367 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729-730 3895 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 113 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92368 M 102 APPEARANCE HEAD TILT LEFT MALOCCLUSION 701, 706, 708, 712 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659 666, 673, 680, 687, 694, 701, 706, 708, 712 EXCRETION FEW FECES NO FECES 706, 708 712 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 MASS(ES) AXILLARY REGION-RIGHT WEEK 91 VENTRAL-ABDOMINAL WEEK 100 631, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C 706C, 708C, 712C 694C, 701C, 706C, 708C, 712C 3896 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 114 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92369 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344 MASS(ES) AXILLARY REGION-LEFT WEEK 102 VENTRAL-ABDOMINAL-LEFT WEEK 51 VENTRAL-ABDOMINAL-RIGHT WEEK 51 708C, 715C, 722C, 729-730C 351C, 358C, 428C, 435C, 505C, 512C, 582C, 589C, 659C, 666C, 729-730C 365C, 442C, 519C, 596C, 673C, 372C, 449C, 526C, 603C, 680C, 379C, 456C, 533C, 610C, 687C, 386C, 463C, 540C, 617C, 694C, 393C, 470C, 547C, 624C, 701C, 400C, 477C, 554C, 631C, 708C, 407C, 484C, 561C, 638C, 715C, 414C, 491C, 568C, 645C, 722C 421C 498C 575C 652C 351C, 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C C92370 M 100 APPEARANCE MALOCCLUSION 554, 561, 568 SKIN & PELAGE SORE/SCAB AXILLARY REGION-RIGHT 687-688, 694, 697 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 3897 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 115 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92370 M 100 NORMAL NO REMARKABLE OBSERVATIONS 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 MASS(ES) AXILLARY REGION-RIGHT WEEK 80 VENTRAL-ABDOMINAL-LEFT WEEK 100 VENTRAL-THORACIC WEEK 80 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687-688C, 694C 697C 697C 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687-688C, 694C 697C C92371 D 89 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 596, 603, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 610, 617, 619 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 554, 561, 568, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 575, 582, 589 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 526, 533, 540, 547 3898 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 116 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92372 3 53 APPEARANCE MALOCCLUSION 351, 358 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 365, 367 C92373 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 C92374 T 105 APPEARANCE MALOCCLUSION 106, 197, 288, 379, 470, 561, 652, 113, 204, 295, 386, 477, 568, 659, 120, 211, 302, 393, 484, 575, 666, 127, 218, 309, 400, 491, 582, 673, 134, 225, 316, 407, 498, 589, 680, 141, 232, 323, 414, 505, 596, 687, 148, 239, 330, 421, 512, 603, 694, 155, 246, 337, 428, 519, 610, 701, 162, 253, 344, 435, 526, 617, 708, 169, 260, 351, 442, 533, 624, 715, 176, 267, 358, 449, 540, 631, 722, 183, 190 274, 281 365, 372 456, 463 547, 554 638, 645 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 365, 372C, 379C, 386C, 393C, 400C 3899 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 117 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92375 M 87 APPEARANCE HUNCHED 601, 603, 605 DISCHARGE NASAL RED IN COLOR 601, 603, 605 EXCRETION FEW FECES NO FECES 596 601, 603, 605 EYES RED DISCHARGE EYE-LEFT 601, 603, 605 SKIN & PELAGE YELLOW HAIR COAT 589, 596, 601, 603, 605 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 3900 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 118 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92376 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309 MASS(ES) VENTRAL-CERVICAL WEEK 46 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C, 367C C92377 D 57 APPEARANCE MALOCCLUSION 64, 71, 78, 85, 92, 99, 106, 113, 120, 127, 134, 141, 148, 155 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337 344, 351, 358, 365, 372, 379, 386, 393 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 31 211, 218, 225, 232, 239C, 246C, 253C, 260C, 267C, 274C, 281C 288C, 295C, 302C, 309C, 316C, 323C, 330C, 337C, 344C, 351C, 358C 365C, 372C, 379C, 386C, 393C 3901 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 119 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92378 M 73 APPEARANCE MALOCCLUSION THIN 190 505, 508 BEHAVIOR ATAXIC <UNSPECIFIED> 508 HYPOACTIVE <UNSPECIFIED> 508 EXCRETION FEW FECES 505, 508 EYES RED DISCHARGE EYES 508 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL YELLOW HAIR COAT 505, 508 508 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 211, 218, 316, 323, 407, 414, 498 22, 29, 36, 43, 50, 127, 134, 141, 148, 225, 232, 239, 246, 330, 337, 344, 351, 421, 428, 435, 442, 57, 64, 71, 78, 85, 155, 162, 169, 176, 253, 260, 267, 274, 358, 365, 372, 379, 449, 456, 463, 470, 92, 99, 106 183, 197, 204 281, 288, 295 386, 393, 400 477, 484, 491 3902 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 120 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92378 M 73 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 302, 309 C92379 D 72 APPEARANCE THIN 491, 498 EXCRETION FEW FECES NO FECES 491, 498 449 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 VENTRAL-CERVICAL WEEK 65 VENTRAL-THORACIC WEEK 70 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414C 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C 498C 449C 484C, 491C, 498C 3903 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 121 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92380 M 81 APPEARANCE MALOCCLUSION THIN 106, 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554 559-561 554, 559-561 BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> 554, 560-561 559 EXCRETION FEW FECES 554, 559-561 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99 MASS(ES) AXILLARY REGION-LEFT WEEK 45 AXILLARY REGION-RIGHT WEEK 60 VENTRAL-ABDOMINAL-LEFT WEEK 50 309, 316, 323, 330, 337, 344C, 351C, 358C, 365C, 372C, 379C 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C 463C, 470C, 477C 414, 421, 428, 435, 442, 449, 456, 463C, 470C, 477C, 484C, 491C 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 559-561C 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C 3904 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 122 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92381 M 69 APPEARANCE THIN 477, 479-480 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 480 SENSITIVE TO TOUCH 477 DISCHARGE NASAL RED IN COLOR 477, 479-480 EXCRETION FEW FECES NO FECES 463, 470, 477, 479 480 EYES RED DISCHARGE EYES 477, 479-480 SKIN & PELAGE YELLOW HAIR COAT 463, 470, 477, 479-480 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414 421, 428, 435, 442, 449, 456 3905 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 123 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92381 M 69 MASS(ES) VENTRAL-CERVICAL WEEK 46 316, 323, 330, 337, 344C C92382 M 57 APPEARANCE THIN 386, 393 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 393 EXCRETION FEW FECES 393 EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL-RIGHT 386, 393 386, 393 SKIN & PELAGE BROWN HAIR COAT GENERALIZED 393 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 3906 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 124 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92383 T 105 APPEARANCE MALOCCLUSION 29, 36 SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL 701, 708, 715, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113, 120 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659 MASS(ES) AXILLARY REGION-LEFT WEEK 96 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729-730C C92384 M 88 APPEARANCE THIN 613-614 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 613-614 EXCRETION FEW FECES NO FECES 610, 613 614 3907 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 125 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92384 M 88 EYES RED DISCHARGE EYES 610, 613-614 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL PALE ENTIRE BODY 613-614 614 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C92385 M 96 APPEARANCE HEAD TILT LEFT THIN 658-659, 666 666 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 589, 593, 596, 603 DISCHARGE NASAL RED IN COLOR 658-659, 666 3908 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 126 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92385 M 96 EXCRETION FEW FECES 596, 603, 610, 617, 624, 631, 638, 645, 652 NO FECES 589, 593, 658-659, 666 EYES RED DISCHARGE EYES 593, 596, 603, 610, 617 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 582, 589, 593, 596, 603, 610, 617, 624, 631 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C92386 T 105 APPEARANCE THIN 701, 708, 715, 722, 729-730 EXCRETION FEW FECES 638, 645, 652, 659, 666, 673, 680, 687 SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT VENTRAL-CERVICAL-LEFT 582, 589 701, 708, 715, 722, 729-730 3909 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 127 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92386 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519 MASS(ES) AXILLARY REGION-LEFT WEEK 76 INGUINAL REGION-LEFT WEEK 101 VENTRAL-ABDOMINAL WEEK 80 VENTRAL-CERVICAL-LEFT WEEK 95 526, 533, 540, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 701C, 708C, 715C, 722C, 729-730C 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C 708C, 715C, 722C, 729-730C 659, 666, 673, 680, 687, 694, 701, 708, 715C, 722C, 729-730C WART LIKE LESION(S) EAR-LEFT WEEK 92 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729-730 3910 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 128 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92387 M 74 APPEARANCE THIN 512-513 EYES SQUINTED PERI-ORBITAL 513 SKIN & PELAGE RED HAIR COAT PERINEAL AREA 513 VENTRAL-ABDOMINAL 513 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 365, 372, 379, 386, 393 400 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 VENTRAL-ABDOMINAL-LEFT WEEK 51 407, 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C 484C, 491C, 498C, 505C, 512-513C 351, 358 3911 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 129 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92388 3 53 SKIN & PELAGE SORE/SCAB HEAD-ENTIRE 309, 316 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 323, 330, 337, 344, 351, 358, 365, 367 C92389 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C92390 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C92391 D 93 EXCRETION FEW FECES 601, 603 EYES RED DISCHARGE EYE-LEFT 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554 561, 568, 575, 582, 589, 596, 601, 603, 610, 617, 624, 631, 638 645, 647 3912 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 130 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92391 D 93 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330 MASS(ES) AXILLARY REGION-LEFT WEEK 61 INGUINAL REGION-RIGHT WEEK 84 VENTRAL-ABDOMINAL-LEFT WEEK 49 VENTRAL-ABDOMINAL-RIGHT WEEK 84 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C 575C, 582C, 589C, 596C, 601C, 603C, 610C, 617C, 624C, 631C, 638C 645C, 647C 582C, 589C, 596C, 601C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 647C 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414 582C, 589C, 596C, 601C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 647C C92392 M 64 APPEARANCE HEAD TILT LEFT 435, 442 DISCHARGE NASAL RED IN COLOR 365, 435, 442 EYES DISCOLORED/LIGHT RED EYES 435, 442 3913 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 131 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92392 M 64 EYES RED DISCHARGE EYES 365, 372, 379, 386, 393, 400, 442 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 407, 414, 421 428 C92393 M 94 APPEARANCE MALOCCLUSION THIN 653 638, 645, 652-653 SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL 50, 57, 64, 71, 239, 246, 253, 260, 267, 274, 302, 309, 316, 344 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435 442, 449, 456, 463, 470, 477, 484, 491, 498 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 78, 85, 92, 99, 106, 113, 120, 127 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218 225, 232, 281, 288, 295, 323, 330, 337 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C 645C, 652-653C 3914 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 132 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92393 M 94 MASS(ES) INGUINAL REGION-LEFT WEEK 92 638C, 645C, 652-653C VENTRAL-ABDOMINAL-LEFT WEEK 73 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652-653C C92394 M 85 APPEARANCE THIN 568, 570, 575, 582, 589 DISCHARGE NASAL RED IN COLOR 568, 570, 575, 582, 589 EYES RED DISCHARGE EYE-LEFT 568, 570, 575 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239 MASS(ES) VENTRAL-CERVICAL WEEK 36 246C, 253C, 260C, 267C, 274C, 281C, 288C, 295C, 302C, 309C, 316C 323C, 330C, 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C 554C, 561C, 568C, 570C, 575C, 582C, 589C 3915 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 133 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F8 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92395 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C92396 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92397 2 14 EYES DISPLACED PUPIL EYE-RIGHT 29, 92-93 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 36, 43, 50, 57, 64, 71, 78, 85 C92398 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92399 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92400 2 14 APPEARANCE MALOCCLUSION 78, 85, 92-93 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71 3916 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 134 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92331 F8 288 42 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 10 X 10MM 295 43 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 10 X 10MM 302 44 TO 407 59 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 20 X 10MM 414 60 TO 484 70 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 20 X 15MM 491 71 TO 512 74 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 20 X 20MM 519 75 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 30 X 30MM 526 76 TO 540 78 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 35 X 35MM 547 79 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 50 X O55MM 3917 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 135 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92331 F8 554 80 TO 568 82 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 50 X 55MM 575 83 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 25 X 55MM 582 84 TO 631 91 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 60 X 55MM 638 92 TO 641 92 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 60 X 40MM C92336 F8 407 59 MASS(ES) AXILLARY REGION-LEFT WEEK 59 10 X 10MM 414 60 MASS(ES) AXILLARY REGION-LEFT WEEK 59 20 X 15MM 421 61 TO 442 64 MASS(ES) AXILLARY REGION-LEFT WEEK 59 25 X 25MM 449 65 TO 477 69 MASS(ES) AXILLARY REGION-LEFT WEEK 59 35 X 35MM 3918 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 136 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92336 F8 484 70 TO 512 74 MASS(ES) AXILLARY REGION-LEFT WEEK 59 45 X 45MM 519 75 TO 540 78 MASS(ES) AXILLARY REGION-LEFT WEEK 59 55 X 55MM 547 79 TO 570 82 MASS(ES) AXILLARY REGION-LEFT WEEK 59 60 X 70MM 519 75 MASS(ES) AXILLARY REGION-RIGHT WEEK 75 15 X 15MM 526 76 TO 540 78 MASS(ES) AXILLARY REGION-RIGHT WEEK 75 20 X 20MM 547 79 TO 570 82 MASS(ES) AXILLARY REGION-RIGHT WEEK 75 30 X 35MM C92337 F8 274 40 DISCHARGE OTHER MUCOID DISCHARGE FROM RECTUM 344 50 TO 470 68 MASS(ES) VENTRAL-ABDOMINAL WEEK 50 20 X 20MM 3919 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 137 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92337 F8 477 69 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL WEEK 50 25 X 30MM 505 73 TO 512 74 MASS(ES) VENTRAL-ABDOMINAL WEEK 50 35 X 30MM 519 75 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL WEEK 50 35 X 20MM 547 79 TO 569 82 MASS(ES) VENTRAL-ABDOMINAL WEEK 50 35 X 30MM 505 73 TO 512 74 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 10 X 10MM C92339 F8 260 38 TO 274 40 MASS(ES) SHOULDER-LEFT WEEK 38 20 X 15MM 281 41 TO 337 49 MASS(ES) SHOULDER-LEFT WEEK 38 20 X 20MM 344 50 TO 358 52 MASS(ES) SHOULDER-LEFT WEEK 38 20 X 10MM 3920 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 138 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92339 F8 470 68 TO 477 69 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 15 X 15MM 484 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 20 X 20MM 491 71 TO 512 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 25 X 25MM 519 75 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 40 X 50MM 526 76 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 45 X 60MM 533 77 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 55 X 35MM 547 79 TO 575 83 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 70 X 50MM 582 84 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 75 X 60MM 3921 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 139 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92339 F8 610 88 TO 652 94 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 90 X 80MM C92340 F8 547 79 TO 548 79 MASS(ES) INGUINAL REGION-LEFT WEEK 79 30 X 35MM 512 74 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL WEEK 74 20 X 20MM 547 79 TO 548 79 MASS(ES) VENTRAL-ABDOMINAL WEEK 74 35 X 30MM 344 50 TO 358 52 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 10 X 10MM 365 53 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 20 X 15MM 372 54 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 25 X 20MM 379 55 TO 386 56 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 25 X 30MM 3922 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 140 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92340 F8 393 57 TO 407 59 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 40 X 30MM 414 60 TO 442 64 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 50 X 50MM 449 65 TO 477 69 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 50 X 65MM 484 70 TO 491 71 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 70 X 70MM 498 72 TO 519 75 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 80 X 70MM 526 76 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 85 X 80MM 547 79 TO 548 79 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 100 X 85MM 512 74 TO 540 78 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 74 20 X 20MM 3923 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 141 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92340 F8 547 79 TO 548 79 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 74 35 X 20MM C92341 F8 365 53 TO 367 53 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 10 X 10MM C92343 F8 127 19 MASS(ES) SHOULDER-RIGHT WEEK 19 10 X 15MM 134 20 TO 162 24 MASS(ES) SHOULDER-RIGHT WEEK 19 15 X 20MM 169 25 TO 176 26 MASS(ES) SHOULDER-RIGHT WEEK 19 30 X 30MM 183 27 TO 218 32 MASS(ES) SHOULDER-RIGHT WEEK 19 30 X 40MM 225 33 TO 253 37 MASS(ES) SHOULDER-RIGHT WEEK 19 40 X 55MM 260 38 TO 281 41 MASS(ES) SHOULDER-RIGHT WEEK 19 50 X 40MM 3924 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 142 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92343 F8 288 42 TO 302 44 MASS(ES) SHOULDER-RIGHT WEEK 19 65 X 50MM 309 45 MASS(ES) SHOULDER-RIGHT WEEK 19 60 X 70MM 316 46 TO 330 48 MASS(ES) SHOULDER-RIGHT WEEK 19 65 X 50MM 337 49 TO 400 58 MASS(ES) SHOULDER-RIGHT WEEK 19 65 X 60MM C92348 F8 701 101 TO 708 102 MASS(ES) AXILLARY REGION-RIGHT WEEK 101 20 X 20MM 715 103 MASS(ES) AXILLARY REGION-RIGHT WEEK 101 40 X 35MM 722 104 MASS(ES) AXILLARY REGION-RIGHT WEEK 101 40 X 35MM 729 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 101 50 X 45MM 3925 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 143 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92348 F8 730 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 101 50 X 45MM 694 100 MASS(ES) VENTRAL-ABDOMINAL WEEK 100 40 X 45MM 701 101 MASS(ES) VENTRAL-ABDOMINAL WEEK 100 45 X 40MM 708 102 MASS(ES) VENTRAL-ABDOMINAL WEEK 100 45 X 35MM 715 103 MASS(ES) VENTRAL-ABDOMINAL WEEK 100 60 X 40MM 722 104 MASS(ES) VENTRAL-ABDOMINAL WEEK 100 60 X 40MM 729 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 100 60 X 40MM 730 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 100 60 X 40MM 3926 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 144 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92349 F8 344 50 TO 400 58 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 50 10 X 10MM C92350 F8 656 94 DISCHARGE OTHER RED ORAL 656 94 DISCHARGE OTHER RED ORAL C92354 F8 610 88 TO 724 104 MASS(ES) AXILLARY REGION-RIGHT WEEK 83 10 X 10MM C92358 F8 610 88 TO 708 102 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 10 X 10MM 715 103 TO 730 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 20 X 20MM C92359 F8 638 92 TO 648 93 MASS(ES) AXILLARY REGION-LEFT WEEK 92 10 X 10MM 547 79 MASS(ES) AXILLARY REGION-RIGHT WEEK 79 20 X 10MM 3927 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 145 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92359 F8 554 80 TO 575 83 MASS(ES) AXILLARY REGION-RIGHT WEEK 79 10 X 10MM 582 84 TO 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 79 25 X 25MM 610 88 TO 648 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 79 30 X 30MM 554 80 TO 575 83 MASS(ES) VENTRAL-ABDOMINAL WEEK 80 10 X 10MM C92360 F8 582 84 TO 631 91 MASS(ES) AXILLARY REGION-LEFT WEEK 77 10 X 10MM 687 99 TO 708 102 MASS(ES) AXILLARY REGION-LEFT WEEK 96 30 X 20MM 715 103 TO 722 104 MASS(ES) AXILLARY REGION-LEFT WEEK 96 55 X 50MM 729 105 TO 730 105 MASS(ES) AXILLARY REGION-LEFT WEEK 96 60 X 60MM 3928 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 146 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92360 F8 638 92 TO 645 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 92 10 X 10MM 652 94 TO 666 96 MASS(ES) AXILLARY REGION-RIGHT WEEK 92 20 X 20MM 673 97 TO 708 102 MASS(ES) AXILLARY REGION-RIGHT WEEK 92 30 X 25MM 715 103 TO 730 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 92 45 X 45MM 463 67 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 25 X 25MM 505 73 TO 512 74 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 35 X 35MM 519 75 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 35 X 50MM 547 79 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 50 X 50MM 3929 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 147 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92360 F8 554 80 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 70 X 60MM 666 96 TO 687 99 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 65 X 45MM 694 100 TO 708 102 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 65 X 50MM 715 103 TO 730 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 80 X 65MM C92361 F8 568 82 TO 575 83 MASS(ES) AXILLARY REGION-LEFT WEEK 80 20 X 20MM 582 84 TO 603 87 MASS(ES) AXILLARY REGION-LEFT WEEK 80 25 X 30MM 610 88 TO 702 101 MASS(ES) AXILLARY REGION-LEFT WEEK 80 40 X 35MM 505 73 TO 519 75 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 10 X 10MM 3930 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 148 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92361 F8 526 76 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 25 X 25MM 547 79 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 30 X 45MM 561 81 TO 575 83 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 50 X 50MM 582 84 TO 589 85 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 60 X 65MM 596 86 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 70 X 65MM 610 88 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 85 X 70MM 666 96 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 90 X 65MM 673 97 TO 694 100 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 90 X 85MM 3931 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 149 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92361 F8 701 101 TO 702 101 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 110 X 100MM C92362 F8 708 102 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 102 10 X 10MM 715 103 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 102 30 X 20MM 722 104 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 102 20 X 10MM 729 105 TO 730 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 102 30 X 30MM C92363 F8 526 76 TO 540 78 MASS(ES) AXILLARY REGION-LEFT WEEK 76 20 X 20MM 547 79 MASS(ES) AXILLARY REGION-LEFT WEEK 76 50 X 55MM 554 80 TO 561 81 MASS(ES) AXILLARY REGION-LEFT WEEK 76 60 X 55MM 3932 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 150 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92363 F8 568 82 MASS(ES) AXILLARY REGION-LEFT WEEK 76 80 X 60MM 575 83 TO 575 83 MASS(ES) AXILLARY REGION-LEFT WEEK 76 80 X 80MM 575 83 MASS(ES) AXILLARY REGION-LEFT WEEK 76 80 X 60MM C92368 F8 638 92 TO 706 101 MASS(ES) AXILLARY REGION-RIGHT WEEK 91 20 X 25MM 708 102 TO 712 102 MASS(ES) AXILLARY REGION-RIGHT WEEK 91 35 X 30MM 694 100 TO 712 102 MASS(ES) VENTRAL-ABDOMINAL WEEK 100 40 X 30MM C92369 F8 708 102 MASS(ES) AXILLARY REGION-LEFT WEEK 102 10 X 10MM 715 103 MASS(ES) AXILLARY REGION-LEFT WEEK 102 25 X 20MM 3933 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 151 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92369 F8 722 104 MASS(ES) AXILLARY REGION-LEFT WEEK 102 25 X 20MM 729 105 MASS(ES) AXILLARY REGION-LEFT WEEK 102 25 X 20MM 730 105 MASS(ES) AXILLARY REGION-LEFT WEEK 102 25 X 20 MM 351 51 TO 414 60 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 10 X 10MM 421 61 TO 442 64 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 20 X 20MM 449 65 TO 477 69 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 30 X 30MM 484 70 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 35 X 35MM 505 73 TO 512 74 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 45 X 40MM 3934 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 152 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92369 F8 519 75 TO 526 76 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 50 X 55MM 533 77 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 51 X 40MM 547 79 TO 589 85 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 65 X 60MM 596 86 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 70 X 60MM 610 88 TO 708 102 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 85 X 70MM 715 103 TO 722 104 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 100 X 110MM 729 105 TO 730 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 115 X 110MM 351 51 TO 407 59 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 51 15 X 15MM 3935 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 153 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92370 F8 554 80 TO 561 81 MASS(ES) AXILLARY REGION-RIGHT WEEK 80 10 X 10MM 568 82 TO 575 83 MASS(ES) AXILLARY REGION-RIGHT WEEK 80 20 X 20MM 582 84 TO 589 85 MASS(ES) AXILLARY REGION-RIGHT WEEK 80 30 X 30MM 596 86 TO 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 80 30 X 40MM 610 88 TO 631 91 MASS(ES) AXILLARY REGION-RIGHT WEEK 80 50 X 50MM 638 92 TO 697 100 MASS(ES) AXILLARY REGION-RIGHT WEEK 80 55 X 55MM 697 100 TO 697 100 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 100 15 X 15MM 554 80 TO 561 81 MASS(ES) VENTRAL-THORACIC WEEK 80 10 X 10MM 3936 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 154 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92370 F8 568 82 TO 603 87 MASS(ES) VENTRAL-THORACIC WEEK 80 30 X 30MM 610 88 TO 697 100 MASS(ES) VENTRAL-THORACIC WEEK 80 45 X 35MM C92374 F8 372 54 TO 400 58 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 10 X 10MM C92376 F8 316 46 TO 367 53 MASS(ES) VENTRAL-CERVICAL WEEK 46 30 X 30MM C92377 F8 239 35 TO 309 45 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 31 15 X 15MM 316 46 TO 393 57 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 31 20 X 20MM C92379 F8 414 60 TO 421 61 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 15 X 10MM 428 62 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 20 X 15MM 3937 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 155 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92379 F8 449 65 MASS(ES) VENTRAL-CERVICAL WEEK 65 10 X 10MM 484 70 TO 498 72 MASS(ES) VENTRAL-THORACIC WEEK 70 10 X 10MM C92380 F8 344 50 TO 379 55 MASS(ES) AXILLARY REGION-LEFT WEEK 45 10 X 10MM 386 56 TO 407 59 MASS(ES) AXILLARY REGION-LEFT WEEK 45 15 X 15MM 414 60 TO 477 69 MASS(ES) AXILLARY REGION-LEFT WEEK 45 20 X 20MM 463 67 TO 498 72 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 20 X 20MM 505 73 TO 519 75 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 40 X 30MM 526 76 TO 540 78 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 45 X 45MM 3938 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 156 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92380 F8 547 79 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 50 X 50MM 554 80 TO 561 81 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 45 X 45MM 344 50 TO 358 52 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 50 10 X 10MM 365 53 TO 407 59 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 50 20 X 30MM C92381 F8 344 50 MASS(ES) VENTRAL-CERVICAL WEEK 46 10 X 10MM C92383 F8 666 96 TO 708 102 MASS(ES) AXILLARY REGION-LEFT WEEK 96 20 X 20MM 715 103 TO 730 105 MASS(ES) AXILLARY REGION-LEFT WEEK 96 40 X 40MM C92386 F8 547 79 MASS(ES) AXILLARY REGION-LEFT WEEK 76 10 X 20MM 3939 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 157 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92386 F8 554 80 TO 652 94 MASS(ES) AXILLARY REGION-LEFT WEEK 76 15 X 10MM 701 101 TO 708 102 MASS(ES) INGUINAL REGION-LEFT WEEK 101 10 X 10MM 715 103 TO 730 105 MASS(ES) INGUINAL REGION-LEFT WEEK 101 30 X 30MM 554 80 TO 575 83 MASS(ES) VENTRAL-ABDOMINAL WEEK 80 10 X 10MM 582 84 MASS(ES) VENTRAL-ABDOMINAL WEEK 80 30 X 25MM 589 85 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL WEEK 80 30 X 30MM 638 92 TO 722 104 MASS(ES) VENTRAL-ABDOMINAL WEEK 80 30 X 40MM 729 105 TO 730 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 80 40 X 40MM 3940 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 158 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92386 F8 715 103 TO 730 105 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 95 10 X 10MM C92387 F8 414 60 TO 435 63 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 10 X 10MM 442 64 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 15 X 15MM 449 65 TO 498 72 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 30 X 30MM 505 73 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 45 X 40MM 512 74 TO 513 74 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 45 X 30MM C92391 F8 421 61 MASS(ES) AXILLARY REGION-LEFT WEEK 61 10 X 10MM 428 62 TO 540 78 MASS(ES) AXILLARY REGION-LEFT WEEK 61 15 X 15MM 3941 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 159 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92391 F8 547 79 TO 647 93 MASS(ES) AXILLARY REGION-LEFT WEEK 61 20 X 30MM 582 84 TO 647 93 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 10 X 10MM 582 84 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 84 10 X 10MM 638 92 TO 647 93 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 84 20 X 15MM C92393 F8 414 60 TO 421 61 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 20 X 20MM 428 62 TO 442 64 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 30 X 30MM 449 65 TO 477 69 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 40 X 40MM 484 70 TO 519 75 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 55 X 50MM 3942 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 160 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92393 F8 526 76 TO 540 78 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 60 X 60MM 547 79 TO 589 85 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 60 X 70MM 596 86 TO 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 70 X 80MM 610 88 TO 652 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 95 X 80MM 653 94 TO 653 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 110 X 90MM 638 92 TO 653 94 MASS(ES) INGUINAL REGION-LEFT WEEK 92 15 X 15MM 505 73 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 10 X 10MM 561 81 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 15 X 15MM 3943 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 161 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92393 F8 638 92 TO 653 94 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 20 X 25MM C92394 F8 246 36 TO 267 39 MASS(ES) VENTRAL-CERVICAL WEEK 36 10 X 10MM 274 40 TO 309 45 MASS(ES) VENTRAL-CERVICAL WEEK 36 15 X 10MM 316 46 TO 365 53 MASS(ES) VENTRAL-CERVICAL WEEK 36 20 X 25MM 372 54 TO 442 64 MASS(ES) VENTRAL-CERVICAL WEEK 36 25 X 25MM 449 65 TO 498 72 MASS(ES) VENTRAL-CERVICAL WEEK 36 35 X 35MM 505 73 TO 519 75 MASS(ES) VENTRAL-CERVICAL WEEK 36 45 X 45MM 526 76 TO 540 78 MASS(ES) VENTRAL-CERVICAL WEEK 36 55 X 50MM 3944 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 162 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92394 F8 547 79 TO 589 85 MASS(ES) VENTRAL-CERVICAL WEEK 36 75 X 50MM 3945 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 163 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92401 M 92 APPEARANCE HEAD TILT LEFT MALOCCLUSION THIN 617, 624, 631, 633-634, 638, 641 638, 641 633-634, 638, 641 BEHAVIOR HYPOACTIVE <UNSPECIFIED> COLD TO TOUCH ENTIRE BODY 634, 638, 641 641 EXCRETION FEW FECES NO FECES NONFORMED FECES 617, 624, 631, 633 633-634 547 EYES RED DISCHARGE EYE-LEFT 634 EYES 641 SKIN & PELAGE PALE ENTIRE BODY 638, 641 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 3946 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 164 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92401 M 92 NORMAL NO REMARKABLE OBSERVATIONS 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 554, 561, 568 575, 582, 589, 596, 603, 610 C92402 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 C92403 D 51 DISCHARGE GENITAL RED IN COLOR 260 EYES RED DISCHARGE EYE-RIGHT 155, 162 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 169, 176, 183, 190, 197, 204, 211 218, 225, 232 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 239C, 246C, 253C, 260C, 267C, 274C, 281C, 288C, 295C, 302C, 309C 316C, 323C, 330C, 337C, 344C, 351C, 356C 3947 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 165 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92404 M 81 EYES SQUINTED PERI-ORBITAL 566 SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL-LEFT YELLOW HAIR COAT 554, 561, 566 566 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 VENTRAL-ABDOMINAL-RIGHT WEEK 79 VENTRAL-CERVICAL-LEFT WEEK 70 526C, 533C, 540C, 547C, 554C, 561C, 566C 547, 554, 561, 566 484, 491, 498, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C 561C, 566C 3948 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 166 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92405 M 98 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 686 COLD TO TOUCH ENTIRE BODY 686 DISCHARGE NASAL RED IN COLOR 666, 668, 673 EXCRETION FEW FECES NO FECES NONFORMED FECES 664-666 668, 673 582, 589 EYES SQUINTED PERI-ORBITAL 668, 673, 680, 686 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA 668, 673, 680, 686 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 631, 638, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 645, 652, 659 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 610, 617, 624 3949 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 167 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92405 M 98 MASS(ES) AXILLARY REGION-LEFT WEEK 79 547, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C INGUINAL REGION-LEFT WEEK 84 582C, 589C, 596C, 603C C92406 3 53 APPEARANCE MALOCCLUSION 176, 183, 190, 197, 204, 211, 218, 225, 232 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 239, 246, 253, 260 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358, 365, 367 C92407 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C92408 M 104 EYES PALE EYES 722 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 3950 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 168 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92408 M 104 MASS(ES) AXILLARY REGION-RIGHT WEEK 73 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C VENTRAL-ABDOMINAL-RIGHT WEEK 92 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C 715C, 722C C92409 M 99 APPEARANCE HEAD TILT RIGHT 690-691 BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> 690-691 691 EXCRETION FEW FECES 679-680 EYES RED DISCHARGE EYES 673, 679-680, 687, 690-691 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 673, 679-680, 687, 690-691 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 3951 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 169 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92409 M 99 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 491, 498, 505 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 VENTRAL-ABDOMINAL-LEFT WEEK 66 WEEK 75 512, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C 666C, 673C, 679-680C, 687C, 690-691C 456, 463, 470, 477, 484 519C, 526C, 533C, 540C, 547C, 554C C92410 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 103 VENTRAL-CERVICAL WEEK 88 715, 722, 729-730C 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C 687C, 694C, 701C, 708C, 715C, 722C, 729-730C 3952 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 170 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92411 3 53 APPEARANCE MALOCCLUSION 148, 152, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316 323, 330, 337, 344, 351, 358, 365, 367 BEHAVIOR SENSITIVE TO TOUCH 152 DISCHARGE NASAL RED IN COLOR 152 EYES RED DISCHARGE EYE-LEFT 141 SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL-LEFT 309, 316 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 302C, 309C, 316C, 323C, 330C, 337C, 344, 351, 358, 365, 367 3953 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 171 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92412 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729-730 C92413 M 102 APPEARANCE HEAD TILT RIGHT MALOCCLUSION 701, 708, 710 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 710 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 710 EXCRETION FEW FECES 710 RESPIRATION LABORED 701, 708, 710 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 710 3954 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 172 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92413 M 102 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274 MASS(ES) AXILLARY REGION-RIGHT WEEK 96 666C, 673C, 680C, 687C, 694C, 701C, 708C, 710C C92414 M 63 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232 MASS(ES) AXILLARY REGION-LEFT WEEK 42 LIMB-FRONT-RIGHT WEEK 35 PAW-HIND-RIGHT WEEK 42 VENTRAL-ABDOMINAL-RIGHT WEEK 44 288, 295 239, 246, 253, 260C, 267C, 274C, 281C 288C, 295C 302, 309C, 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C, 372C 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C 3955 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 173 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92415 D 89 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 619 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C92416 T 105 SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL SORE/SCAB TAIL TAIL-PROXIMAL 701, 708, 715, 722, 729-730 729-730 519, 526, 638, 645, 652, 701 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 TAIL-PROXIMAL WEEK 75 582C, 589C, 596C, 603C 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729-730C 3956 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 174 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92416 T 105 WART LIKE LESION(S) HEAD-MAXILLARY-RIGHT WEEK 79 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729-730 C92417 M 94 APPEARANCE HEAD TILT LEFT RIGHT THIN 648, 652 653 622, 624, 631, 633-634, 638, 645, 648, 652-653 BEHAVIOR ATAXIC <UNSPECIFIED> CIRCLING LEFT 634 648, 652 EXCRETION FEW FECES NO FECES 596, 603, 610, 617, 622, 624, 631, 633, 648, 652-653 634, 638, 645 EYES RED DISCHARGE EYES 634 SKIN & PELAGE YELLOW HAIR COAT 631, 633-634, 638, 645, 648, 652-653 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 3957 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 175 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92417 M 94 NORMAL NO REMARKABLE OBSERVATIONS 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589 C92418 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C92419 T 105 APPEARANCE MALOCCLUSION 85, 92, 99, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78 MASS(ES) AXILLARY REGION-LEFT WEEK 96 AXILLARY REGION-RIGHT WEEK 101 666, 673, 680, 687, 694, 701, 708, 715, 722, 729-730 701C, 708C, 715C, 722C, 729-730C 3958 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 176 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92420 M 73 SKIN & PELAGE SORE/SCAB AXILLARY REGION-RIGHT 505 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 372, 379 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 VENTRAL-ABDOMINAL WEEK 50 WEEK 56 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C 484C, 491C, 498C, 505C 344, 351, 358, 365 386C, 393C, 400C C92421 T 105 APPEARANCE MALOCCLUSION 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687 694, 701, 708, 715, 722, 729-730 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659 666, 673, 680, 687, 694, 701, 708, 715, 722, 729-730 3959 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 177 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92421 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141 C92422 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 C92423 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 596, 603, 687, 694, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 610, 617, 624, 631, 701, 708, 715, 722, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 638, 645, 652, 659, 729-730 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 582, 589 666, 673, 680 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 80 554, 561, 568, 575 C92424 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 3960 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 178 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92425 M 100 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT 687, 694 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) INGUINAL REGION-LEFT WEEK 92 INGUINAL REGION-RIGHT WEEK 84 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C, 680C, 687C, 694C C92426 T 105 APPEARANCE SWOLLEN PAW-HIND-RIGHT 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708 EYES RED DISCHARGE EYE-LEFT 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659 666, 673, 680, 687, 694, 701, 708, 715, 722, 729-730 3961 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 179 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92426 T 105 SKIN & PELAGE SORE/SCAB DORSAL 701, 708, 715, 722, 729-730 PAWS-HIND 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694, 701, 708, 715, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232 MASS(ES) DORSAL WEEK 83 INGUINAL REGION-RIGHT WEEK 35 VENTRAL-ABDOMINAL-LEFT WEEK 101 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 729-730C 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323 330, 337, 344 701C, 708C, 715C, 722C, 729-730C C92427 M 63 APPEARANCE THIN 428, 435 SKIN & PELAGE SORE/SCAB LATERAL-ABDOMINAL-RIGHT 428, 435 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 3962 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 180 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92427 M 63 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183 MASS(ES) AXILLARY REGION-RIGHT WEEK 28 WEEK 55 190C, 197C, 204C, 211C, 218C, 225C, 232C, 239C, 246C, 253C, 260C 267C, 274C, 281C, 288C, 295C, 302C, 309C, 316C, 323C, 330C, 337C 344C, 351C, 358C, 365C, 372C 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C C92428 M 62 EYES RED DISCHARGE EYES 428 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL-LEFT 428 SORE/SCAB HEAD-CRANIAL 344 VENTRAL-CERVICAL 428 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 MASS(ES) AXILLARY REGION-LEFT WEEK 51 VENTRAL-CERVICAL-LEFT WEEK 38 351C, 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C 428C 260C, 267C, 274C, 281C, 288C, 295C, 302C, 309C, 316C, 323C, 330C 3963 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 181 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92428 M 62 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 38 337C C92429 D 62 BEHAVIOR COLD TO TOUCH ENTIRE BODY 393, 400 EYES PALE EYES 393, 400, 407 SKIN & PELAGE PALE ENTIRE BODY 393, 400, 407, 414 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 421, 428, 431 C92430 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 694, 701, 708, 715, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 3964 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 182 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92430 T 105 NORMAL NO REMARKABLE OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687 MASS(ES) VENTRAL-ABDOMINAL WEEK 102 708C, 715C, 722C, 729-730C C92431 T 105 SKIN & PELAGE SORE/SCAB LATERAL-CERVICAL-LEFT 29, 36, 43, 50, 57 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 141, 148, 232, 239, 323, 330, 414, 421, 505, 512, 596, 603, 22, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106, 113, 120, 127, 134 183, 190, 197, 204, 211, 218, 225 274, 281, 288, 295, 302, 309, 316 365, 372, 379, 386, 393, 400, 407 456, 463, 470, 477, 484, 491, 498 547, 554, 561, 568, 575, 582, 589 638, 645, 652 MASS(ES) AXILLARY REGION-RIGHT WEEK 96 INGUINAL REGION-LEFT WEEK 95 666, 673, 680, 687C, 694C, 701C, 708C, 715C, 722C, 729-730C 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 729-730C 3965 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 183 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92432 M 77 APPEARANCE HUNCHED MALOCCLUSION 519, 526, 533, 537 29, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 484 491, 498, 505, 512, 519, 526, 533, 537 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 537 RESPIRATION LABORED 537 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 65 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C 526C, 533C, 537C C92433 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 3966 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 184 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92434 D 91 APPEARANCE THIN 582, 589, 596, 603, 610, 617, 624, 631, 633 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372 MASS(ES) SHOULDER-RIGHT WEEK 55 VENTRAL-ABDOMINAL-LEFT WEEK 61 VENTRAL-ABDOMINAL-RIGHT WEEK 70 WEEK 74 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C 533C, 540C, 547C, 554C, 561C, 568C, 570C, 575C, 582C, 589C, 596C 603C, 610C, 617C, 624C, 631C, 633C 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505C 512 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C 561C, 568C, 570C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C 631C, 633C 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 570C, 575C 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 633C 3967 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 185 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92435 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 C92436 M 96 APPEARANCE MALOCCLUSION THIN 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 638, 645, 652, 659, 666 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218 MASS(ES) AXILLARY REGION-LEFT WEEK 74 VENTRAL-ABDOMINAL-LEFT WEEK 66 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C 666C 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C 3968 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 186 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92437 M 50 APPEARANCE THIN 344, 348 BEHAVIOR ATAXIC <UNSPECIFIED> 344, 348 EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL 344, 348 344, 348 SKIN & PELAGE RED HAIR COAT DORSAL 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274 281, 288, 295, 302, 309, 316, 323, 330, 337 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 34 232C, 239C, 246C, 253C, 260C, 267C, 274C, 281C, 288C, 295C, 302C 309C, 316C, 323C, 330C, 337C, 344C, 348C 3969 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 187 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92438 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295 MASS(ES) VENTRAL-ABDOMINAL WEEK 44 302C, 309C, 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C, 367C C92439 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729-730 C92440 D 80 APPEARANCE LIMITED USE LIMB-HIND-LEFT SWOLLEN PAW-HIND-LEFT 547, 554, 559-560 559 EXCRETION FEW FECES 559 SKIN & PELAGE SORE/SCAB HEAD-MAXILLARY-RIGHT 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 3970 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 188 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92440 D 80 SKIN & PELAGE SORE/SCAB HEAD-MAXILLARY-RIGHT 519, 526, 533, 540, 547, 554, 559-560 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 HEAD-MAXILLARY-RIGHT WEEK 66 VENTRAL-ABDOMINAL-RIGHT WEEK 45 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 456, 463, 470, 477, 484, 491, 498, 505C, 512C, 519C, 526C, 533C 540C, 547C, 554C, 559-560C 309, 316, 323, 330, 337, 344 C92441 3 53 SKIN & PELAGE SORE/SCAB DORSAL 316, 323, 330, 337, 344, 351, 358, 365, 367 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260 MASS(ES) AXILLARY REGION-RIGHT WEEK 39 267, 274, 281, 288, 295, 302, 309, 316, 323 3971 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 189 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92441 3 53 MASS(ES) INGUINAL REGION-LEFT WEEK 48 330C, 337C, 344C, 351C, 358C, 365C, 367C C92442 M 68 BEHAVIOR HYPOACTIVE <UNSPECIFIED> MILD 476 472, 476 DISCHARGE NASAL RED IN COLOR 472, 476 EXCRETION NO FECES 470, 472, 476 EYES RED DISCHARGE EYES 476 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT 470, 472, 476 470, 472, 476 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463 3972 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 190 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92443 T 105 EXCRETION NONFORMED FECES 582, 589, 596, 603 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 372, 379, 463, 470, 554, 561, 673, 680, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 386, 393, 400, 407, 477, 484, 491, 498, 568, 575, 610, 617, 687, 694, 701, 708, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 414, 421, 428, 435, 505, 512, 519, 526, 624, 631, 638, 645, 715, 722, 729-730 92, 99, 106 183, 190, 197 351, 358, 365 442, 449, 456 533, 540, 547 652, 659, 666 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 40 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344 C92444 T 105 SKIN & PELAGE SORE/SCAB PERINEAL AREA 708, 715, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547 MASS(ES) INGUINAL REGION-LEFT WEEK 80 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 687, 694, 701, 708C, 715C, 722C 3973 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 191 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92444 T 105 MASS(ES) INGUINAL REGION-LEFT WEEK 80 729-730C INGUINAL REGION-RIGHT WEEK 92 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C 715C, 722C, 729-730C VENTRAL-ABDOMINAL-RIGHT WEEK 101 701C, 708C, 715C, 722C, 729-730C C92445 M 96 APPEARANCE OBESE 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603 610, 617, 624, 631, 638, 645, 652, 659, 663-666 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 666 DISCHARGE NASAL RED IN COLOR 663, 666 EXCRETION FEW FECES 663-666 EYES RED DISCHARGE EYE-LEFT 663 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA 663-666 3974 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 192 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92445 M 96 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421 C92446 M 83 APPEARANCE HEAD TILT RIGHT THIN 575, 579 579 BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> 579 568, 575, 579 DISCHARGE NASAL RED IN COLOR 554, 559, 561, 568, 575, 579 EXCRETION FEW FECES NO FECES 559, 561 568, 575, 579 EYES RED DISCHARGE EYES 554, 559, 561, 568, 575, 579 3975 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 193 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92446 M 83 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA 579 VENTRAL-ABDOMINAL 561, 568, 575, 579 RED HAIR COAT HEAD-CRANIAL 559 YELLOW HAIR COAT 559 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547 C92447 M 92 APPEARANCE THIN 582, 589, 596, 603, 610, 617, 624, 631, 638 BEHAVIOR ATAXIC <UNSPECIFIED> 638 COLD TO TOUCH ENTIRE BODY 638 EXCRETION FEW FECES 638 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 3976 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 194 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92447 M 92 NORMAL NO REMARKABLE OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575 C92448 M 70 APPEARANCE HEAD TILT RIGHT MALOCCLUSION 477, 484 323, 484 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 C92449 3 53 EYES RED DISCHARGE EYE-LEFT 197, 204, 302, 309, 316, 323, 330, 337, 344 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 211 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 351 358, 365, 367 3977 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 195 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92450 M 89 APPEARANCE HEAD TILT LEFT THIN 610, 617, 619 610, 617, 619 BEHAVIOR ATAXIC <UNSPECIFIED> 619 EXCRETION FEW FECES 594, 596, 603, 610, 617, 619 SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL 547, 554, 561, 568, 575, 582, 589, 594, 596, 603, 610, 617, 619 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 414, 421, 428 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 MASS(ES) INGUINAL REGION-RIGHT WEEK 40 VENTRAL-ABDOMINAL WEEK 75 VENTRAL-ABDOMINAL-LEFT WEEK 50 VENTRAL-CERVICAL WEEK 75 274, 281, 288, 295, 302, 309, 316, 323, 330, 337 519, 526, 533, 540, 547, 554C, 561C, 568C, 575C, 582C, 589C 594C, 596C, 603C, 610C, 617C, 619C 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 519, 526, 533, 540, 547, 554, 561, 568, 575 3978 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 196 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92450 M 89 MASS(ES) VENTRAL-CERVICAL WEEK 77 533, 540, 547, 554, 561, 568, 575 WEEK 84 582C, 589C, 594C, 596C, 603C, 610C, 617C, 619C C92451 T 105 APPEARANCE SWOLLEN PAW-HIND-RIGHT PAWS-HIND 638, 645, 652, 659 666, 673, 680, 687, 694, 701, 708, 715, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 414, 421, 505, 512, 596, 603, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 456, 463, 470, 477, 547, 554, 561, 568, 92, 99, 106 183, 190, 197 274, 281, 407 484, 491, 498 575, 582, 589 MASS(ES) AXILLARY REGION-LEFT WEEK 48 INGUINAL REGION-RIGHT WEEK 42 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 288, 295, 302, 309, 316, 323, 330, 337 C92452 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729-730 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 3979 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 197 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92452 T 105 NORMAL NO REMARKABLE OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 C92453 M 100 EXCRETION FEW FECES 649, 652, 659, 666 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL 649, 652, 659, 666, 673, 680, 687, 694 649, 652, 659, 666, 673, 680, 687, 694 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 512, 519, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 456, 526, 533, 540, 547 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 463, 470, 477, 484, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 491, 498, 505 MASS(ES) VENTRAL-ABDOMINAL WEEK 61 VENTRAL-CERVICAL WEEK 80 421, 428, 435, 442, 449 554, 561, 568, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 649C, 652C, 659C, 666C, 673C, 680C, 687C, 694C 3980 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 198 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92454 M 81 BEHAVIOR ATAXIC <UNSPECIFIED> 566 EXCRETION FEW FECES 561, 566 EYES SQUINTED PERI-ORBITAL 566 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 566 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 61 VENTRAL-CERVICAL WEEK 54 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 566C 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C 526C, 533C, 540C, 547C, 554C, 561C, 566C 3981 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 199 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92455 M 103 APPEARANCE HEAD TILT LEFT 718 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 718 EXCRETION FEW FECES NO FECES 708, 715, 718 706 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SHOULDER-LEFT 701, 706, 708, 715, 718 706, 708, 715, 718 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 MASS(ES) AXILLARY REGION-LEFT WEEK 101 701C, 706C, 708C, 715C, 718C 3982 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 200 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92456 T 105 EXCRETION NONFORMED FECES 722 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) AXILLARY REGION-RIGHT WEEK 88 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C 687C, 694C, 701C, 708C, 715C, 722C, 729-730C C92457 M 85 APPEARANCE HEAD TILT RIGHT THIN 582, 589 582, 589 EXCRETION FEW FECES 561, 568 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT 582, 589 568 568, 575 3983 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 201 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92457 M 85 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 MASS(ES) AXILLARY REGION-LEFT WEEK 84 INGUINAL REGION-LEFT WEEK 80 582, 589 554, 561, 568, 575 C92458 M 68 APPEARANCE HUNCHED THIN 465, 470 465, 470 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 470 RECUMBENT LATERAL 470 EXCRETION FEW FECES NO FECES 443, 449, 456, 463, 465, 470 449, 465 EYES RED DISCHARGE EYE-LEFT EYES 428, 435, 439, 442-443, 449, 456, 463, 465 465, 470 3984 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 202 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92458 M 68 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA 443, 449, 456, 463, 465, 470 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 407, 414, 421 MASS(ES) AXILLARY REGION-LEFT WEEK 51 351, 358, 365, 372, 379, 386, 393, 400 C92459 M 100 EXCRETION NONFORMED FECES 582, 589, 596, 603 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) AXILLARY REGION-RIGHT WEEK 70 INGUINAL REGION-RIGHT WEEK 92 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C 3985 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 203 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92460 M 97 APPEARANCE HUNCHED THIN 679 666, 673, 679 BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> 679 666, 673, 679 EXCRETION FEW FECES 679 NONFORMED FECES 575 EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL-LEFT 666, 673, 679 638, 645, 652, 659, 666, 673, 679 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA SORE/SCAB PAW-HIND-RIGHT 505, 512 638, 645, 652, 659, 666, 673, 679 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421 3986 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 204 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92460 M 97 MASS(ES) VENTRAL-ABDOMINAL WEEK 62 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C, 679C VENTRAL-ABDOMINAL-RIGHT WEEK 64 442C, 449C C92461 M 61 BEHAVIOR HYPOACTIVE <UNSPECIFIED> SENSITIVE TO TOUCH 425 424-425 DISCHARGE NASAL RED IN COLOR 421, 424-425 EXCRETION FEW FECES 421, 424-425 EYES RED DISCHARGE EYES 424-425 SKIN & PELAGE PALE ENTIRE BODY 424-425 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 3987 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 205 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92461 M 61 NORMAL NO REMARKABLE OBSERVATIONS 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414 C92462 M 99 APPEARANCE THIN 673, 680, 687 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 687 EXCRETION FEW FECES 687 EYES RED DISCHARGE EYES 687 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 3988 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 206 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F9 DOSE: 1 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92463 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 C92464 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92465 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92466 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92467 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 C92468 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92469 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92470 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 3989 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 207 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92403 F9 239 35 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 10 X 10MM 246 36 TO 253 37 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 20 X 10MM 260 38 TO 281 41 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 25 X 25MM 288 42 TO 302 44 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 40 X 40MM 309 45 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 45 X 40MM 316 46 TO 337 49 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 15 X 15MM 344 50 TO 356 51 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 10 X 10MM C92404 F9 526 76 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 35 X 20MM 3990 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 208 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92404 F9 547 79 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 40 X 30MM 554 80 TO 566 81 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 50 X 45MM 505 73 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 70 10 X 10MM 512 74 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 70 15 X 15MM 519 75 TO 540 78 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 70 20 X 15MM 547 79 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 70 35 X 35MM 554 80 TO 566 81 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 70 25 X 25MM C92405 F9 554 80 TO 603 87 MASS(ES) AXILLARY REGION-LEFT WEEK 79 10 X 10MM 3991 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 209 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92405 F9 582 84 TO 603 87 MASS(ES) INGUINAL REGION-LEFT WEEK 84 20 X 20MM C92408 F9 505 73 MASS(ES) AXILLARY REGION-RIGHT WEEK 73 10 X 10MM 512 74 MASS(ES) AXILLARY REGION-RIGHT WEEK 73 15 X 15MM 519 75 TO 547 79 MASS(ES) AXILLARY REGION-RIGHT WEEK 73 20 X 25MM 554 80 TO 575 83 MASS(ES) AXILLARY REGION-RIGHT WEEK 73 30 X 35MM 582 84 TO 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 73 35 X 40MM 610 88 TO 666 96 MASS(ES) AXILLARY REGION-RIGHT WEEK 73 65 X 45MM 673 97 TO 687 99 MASS(ES) AXILLARY REGION-RIGHT WEEK 73 65 X 55MM 3992 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 210 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92408 F9 694 100 TO 708 102 MASS(ES) AXILLARY REGION-RIGHT WEEK 73 70 X 70MM 715 103 MASS(ES) AXILLARY REGION-RIGHT WEEK 73 85 X 85MM 722 104 TO 722 104 MASS(ES) AXILLARY REGION-RIGHT WEEK 73 100 X 85MM 722 104 MASS(ES) AXILLARY REGION-RIGHT WEEK 73 100 X 85MM 638 92 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 92 30 X 40MM 687 99 TO 708 102 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 92 10 X 10MM 715 103 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 92 30 X 20MM 722 104 TO 722 104 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 92 35 X 20MM 3993 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 211 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92408 F9 722 104 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 92 35 X 20MM C92409 F9 519 75 TO 547 79 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 10 X 10MM 554 80 TO 568 82 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 15 X 15MM 575 83 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 15 X 20MM 582 84 TO 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 20 X 20MM 610 88 TO 631 91 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 25 X 25MM 638 92 TO 673 97 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 30 X 30MM 679 97 TO 691 99 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 40 X 40MM 3994 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 212 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92409 F9 519 75 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 75 20 X 35MM 526 76 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 75 30 X 35MM C92410 F9 729 105 TO 730 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 103 10 X 10MM 610 88 TO 708 102 MASS(ES) VENTRAL-CERVICAL WEEK 88 20 X 25MM 715 103 TO 722 104 MASS(ES) VENTRAL-CERVICAL WEEK 88 30 X 30MM 729 105 TO 730 105 MASS(ES) VENTRAL-CERVICAL WEEK 88 40 X 35MM C92411 F9 302 44 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 30 X 10MM 309 45 TO 323 47 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 10 X 10MM 3995 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 213 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92411 F9 330 48 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 15 X 10MM 337 49 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 15 X 10MM C92413 F9 666 96 TO 710 102 MASS(ES) AXILLARY REGION-RIGHT WEEK 96 55 X 50MM C92414 F9 260 38 MASS(ES) LIMB-FRONT-RIGHT WEEK 35 20 X 20MM 267 39 TO 281 41 MASS(ES) LIMB-FRONT-RIGHT WEEK 35 40 X 25MM 288 42 TO 295 43 MASS(ES) PAW-HIND-RIGHT WEEK 42 65 X 45MM 309 45 TO 337 49 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 44 70 X 50MM 344 50 TO 365 53 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 44 70 X 60MM 3996 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 214 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92414 F9 372 54 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 44 90 X 70MM 379 55 TO 407 59 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 44 95 X 80MM 414 60 TO 435 63 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 44 100 X 80MM C92416 F9 582 84 TO 603 87 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 20 X 20MM 519 75 TO 547 79 MASS(ES) TAIL-PROXIMAL WEEK 75 10 X 10MM 554 80 TO 708 102 MASS(ES) TAIL-PROXIMAL WEEK 75 10 X 15MM 715 103 TO 730 105 MASS(ES) TAIL-PROXIMAL WEEK 75 20 X 15MM C92419 F9 701 101 TO 715 103 MASS(ES) AXILLARY REGION-RIGHT WEEK 101 20 X 20MM 3997 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 215 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92419 F9 722 104 TO 730 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 101 25 X 25MM C92420 F9 407 59 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 15 X 15MM 414 60 TO 421 61 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 25 X 20MM 428 62 TO 435 63 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 25 X 30MM 442 64 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 45 X 20MM 449 65 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 35 X 55MM 456 66 TO 470 68 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 35 X 40MM 477 69 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 40 X 50MM 3998 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 216 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92420 F9 484 70 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 40 X 50MM 491 71 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 40 X 50MM 498 72 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 40 X 50MM 505 73 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 55 X 60MM 505 73 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 55 X 60MM 505 73 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 40 X 50MM 386 56 TO 400 58 MASS(ES) VENTRAL-ABDOMINAL WEEK 56 15 X 15MM C92425 F9 638 92 TO 680 98 MASS(ES) INGUINAL REGION-LEFT WEEK 92 25 X 20MM 3999 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 217 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92425 F9 687 99 TO 694 100 MASS(ES) INGUINAL REGION-LEFT WEEK 92 30 X 25MM 582 84 TO 631 91 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 10 X 10MM 638 92 TO 666 96 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 25 X 40MM 673 97 TO 694 100 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 50 X 50MM C92426 F9 575 83 TO 730 105 MASS(ES) DORSAL WEEK 83 10 X 10MM 701 101 TO 708 102 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 101 40 X 40MM 715 103 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 101 60 X 45MM 722 104 TO 730 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 101 70 X 65MM 4000 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 218 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92427 F9 190 28 MASS(ES) AXILLARY REGION-RIGHT WEEK 28 20 X10MM 197 29 TO 204 30 MASS(ES) AXILLARY REGION-RIGHT WEEK 28 20 X 20MM 211 31 TO 218 32 MASS(ES) AXILLARY REGION-RIGHT WEEK 28 30 X 30MM 225 33 TO 232 34 MASS(ES) AXILLARY REGION-RIGHT WEEK 28 45 X 35MM 239 35 TO 281 41 MASS(ES) AXILLARY REGION-RIGHT WEEK 28 50 X 45MM 288 42 TO 309 45 MASS(ES) AXILLARY REGION-RIGHT WEEK 28 75 X 70MM 316 46 TO 337 49 MASS(ES) AXILLARY REGION-RIGHT WEEK 28 110 X 95MM 344 50 TO 365 53 MASS(ES) AXILLARY REGION-RIGHT WEEK 28 100 X 70MM 4001 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 219 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92427 F9 372 54 MASS(ES) AXILLARY REGION-RIGHT WEEK 28 100 X 90MM 379 55 TO 435 63 MASS(ES) AXILLARY REGION-RIGHT WEEK 55 110 X 100MM C92428 F9 351 51 TO 365 53 MASS(ES) AXILLARY REGION-LEFT WEEK 51 20 X 20MM 372 54 MASS(ES) AXILLARY REGION-LEFT WEEK 51 25 X 25MM 379 55 TO 407 59 MASS(ES) AXILLARY REGION-LEFT WEEK 51 40 X 30MM 414 60 TO 421 61 MASS(ES) AXILLARY REGION-LEFT WEEK 51 75 X 60MM 428 62 TO 428 62 MASS(ES) AXILLARY REGION-LEFT WEEK 51 85 X 75MM 428 62 MASS(ES) AXILLARY REGION-LEFT WEEK 51 85 X 75MM 4002 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 220 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92428 F9 260 38 TO 267 39 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 38 20 X 15MM 274 40 TO 281 41 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 38 20 X 20MM 288 42 TO 337 49 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 38 25 X 30MM C92430 F9 708 102 TO 715 103 MASS(ES) VENTRAL-ABDOMINAL WEEK 102 30 X 35MM 722 104 TO 730 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 102 40 X 40MM C92431 F9 687 99 TO 694 100 MASS(ES) AXILLARY REGION-RIGHT WEEK 96 20 X 10MM 701 101 TO 722 104 MASS(ES) AXILLARY REGION-RIGHT WEEK 96 30 X 30MM 729 105 TO 730 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 96 40 X 30MM 4003 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 221 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92431 F9 659 95 TO 666 96 MASS(ES) INGUINAL REGION-LEFT WEEK 95 20 X 20MM 673 97 TO 687 99 MASS(ES) INGUINAL REGION-LEFT WEEK 95 35 X 35MM 694 100 MASS(ES) INGUINAL REGION-LEFT WEEK 95 40 X 40MM 701 101 TO 730 105 MASS(ES) INGUINAL REGION-LEFT WEEK 95 50 X 50MM C92432 F9 449 65 TO 463 67 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 65 10 X 10MM 470 68 TO 477 69 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 65 20 X 20MM 484 70 TO 498 72 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 65 30 X 30MM 505 73 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 65 40 X 40MM 4004 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 222 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92432 F9 512 74 TO 519 75 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 65 45 X 45MM 526 76 TO 537 77 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 65 50 X 45MM C92434 F9 379 55 TO 407 59 MASS(ES) SHOULDER-RIGHT WEEK 55 30 X 35MM 414 60 TO 442 64 MASS(ES) SHOULDER-RIGHT WEEK 55 45 X 35MM 449 65 TO 477 69 MASS(ES) SHOULDER-RIGHT WEEK 55 60 X 40MM 484 70 TO 498 72 MASS(ES) SHOULDER-RIGHT WEEK 55 60 X 50MM 505 73 TO 519 75 MASS(ES) SHOULDER-RIGHT WEEK 55 70 X 55MM 526 76 TO 547 79 MASS(ES) SHOULDER-RIGHT WEEK 55 75 X 55MM 4005 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 223 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92434 F9 554 80 TO 617 89 MASS(ES) SHOULDER-RIGHT WEEK 55 75 X 60MM 624 90 TO 633 91 MASS(ES) SHOULDER-RIGHT WEEK 55 80 X 65MM 505 73 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 10 X 10MM 484 70 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 10 X 10MM 505 73 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 20 X 20MM 512 74 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 25 X 25MM POSTERIOR 547 79 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 30 X 30MM 603 87 TO 617 89 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 35 X 35MM 4006 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 224 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92434 F9 624 90 TO 633 91 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 40 X 50MM ANTERIOR 512 74 TO 519 75 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 15 X 15 ANTERIOR 526 76 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 20 X 20MM 547 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 40 X 30MM 554 80 TO 575 83 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 50 X 40MM 582 84 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 60 X 40MM 603 87 TO 617 89 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 70 X 50MM 624 90 TO 633 91 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 100 X 75MM POSTERIOR 4007 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 225 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92436 F9 512 74 MASS(ES) AXILLARY REGION-LEFT WEEK 74 10 X 10MM 519 75 TO 547 79 MASS(ES) AXILLARY REGION-LEFT WEEK 74 20 X 20MM 554 80 TO 568 82 MASS(ES) AXILLARY REGION-LEFT WEEK 74 35 X 35MM 575 83 MASS(ES) AXILLARY REGION-LEFT WEEK 74 35 X 40MM 582 84 TO 666 96 MASS(ES) AXILLARY REGION-LEFT WEEK 74 40 X 50MM 456 66 TO 470 68 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 66 15 X 10MM 477 69 TO 505 73 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 66 20 X 20MM 512 74 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 66 25 X 30MM 4008 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 226 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92436 F9 519 75 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 66 30 X 30MM 526 76 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 66 35 X 35MM 533 77 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 66 35 X 35MM 540 78 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 66 35 X 35MM 547 79 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 66 35 X 35MM 554 80 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 66 45 X 45MM 638 92 TO 645 93 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 66 50 X 50MM 652 94 TO 666 96 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 66 50 X 60MM 4009 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 227 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92437 F9 232 34 TO 253 37 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 34 20 X 10MM 260 38 TO 281 41 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 34 30 X 15MM 288 42 TO 309 45 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 34 35 X 20MM 316 46 TO 337 49 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 34 45 X 30MM 344 50 TO 348 50 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 34 30 X 20MM C92438 F9 302 44 TO 367 53 MASS(ES) VENTRAL-ABDOMINAL WEEK 44 10 X 10MM C92440 F9 505 73 TO 560 80 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 66 10 X 10MM C92441 F9 330 48 TO 367 53 MASS(ES) INGUINAL REGION-LEFT WEEK 48 20 X 15MM 4010 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 228 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92444 F9 708 102 MASS(ES) INGUINAL REGION-LEFT WEEK 80 10 X 10MM 715 103 TO 730 105 MASS(ES) INGUINAL REGION-LEFT WEEK 80 20 X 20MM 638 92 TO 666 96 MASS(ES) INGUINAL REGION-RIGHT WEEK 92 15 X 15MM 673 97 MASS(ES) INGUINAL REGION-RIGHT WEEK 92 20 X 30MM 680 98 TO 687 99 MASS(ES) INGUINAL REGION-RIGHT WEEK 92 30 X 30MM 694 100 TO 730 105 MASS(ES) INGUINAL REGION-RIGHT WEEK 92 35 X 35MM 701 101 TO 722 104 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 101 40 X 40MM 729 105 TO 730 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 101 55 X 50MM 4011 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 229 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92450 F9 554 80 TO 561 81 MASS(ES) VENTRAL-ABDOMINAL WEEK 75 10 X 10MM 568 82 TO 619 89 MASS(ES) VENTRAL-ABDOMINAL WEEK 75 20 X 20MM 582 84 TO 619 89 MASS(ES) VENTRAL-CERVICAL WEEK 84 30 X 30MM C92453 F9 575 83 TO 631 91 MASS(ES) VENTRAL-CERVICAL WEEK 80 10 X 10MM 638 92 TO 680 98 MASS(ES) VENTRAL-CERVICAL WEEK 80 20 X 30MM 687 99 TO 694 100 MASS(ES) VENTRAL-CERVICAL WEEK 80 30 X 30MM C92454 F9 421 61 TO 442 64 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 61 20 X 15MM 449 65 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 61 40 X 25MM 4012 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 230 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92454 F9 456 66 TO 463 67 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 61 15 X 15MM 470 68 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 61 20 X 20MM 505 73 TO 519 75 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 61 30 X 25MM 526 76 TO 566 81 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 61 25 X 25MM 372 54 MASS(ES) VENTRAL-CERVICAL WEEK 54 10 X 10MM 379 55 TO 407 59 MASS(ES) VENTRAL-CERVICAL WEEK 54 25 X 25MM 414 60 TO 442 64 MASS(ES) VENTRAL-CERVICAL WEEK 54 30 X 30MM 449 65 TO 477 69 MASS(ES) VENTRAL-CERVICAL WEEK 54 40 X 25MM 4013 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 231 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92454 F9 484 70 TO 519 75 MASS(ES) VENTRAL-CERVICAL WEEK 54 40 X 35MM 526 76 TO 566 81 MASS(ES) VENTRAL-CERVICAL WEEK 54 40 X 40MM C92455 F9 701 101 TO 718 103 MASS(ES) AXILLARY REGION-LEFT WEEK 101 45 X 40MM C92456 F9 610 88 TO 631 91 MASS(ES) AXILLARY REGION-RIGHT WEEK 88 10 X 10MM 638 92 TO 687 99 MASS(ES) AXILLARY REGION-RIGHT WEEK 88 25 X 25MM 694 100 TO 708 102 MASS(ES) AXILLARY REGION-RIGHT WEEK 88 45 X 30MM 715 103 TO 730 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 88 45 X 40MM C92459 F9 484 70 TO 498 72 MASS(ES) AXILLARY REGION-RIGHT WEEK 70 10 X 10MM 4014 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 232 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92459 F9 505 73 TO 519 75 MASS(ES) AXILLARY REGION-RIGHT WEEK 70 20 X 20MM 526 76 TO 547 79 MASS(ES) AXILLARY REGION-RIGHT WEEK 70 25 X 25MM 554 80 TO 694 100 MASS(ES) AXILLARY REGION-RIGHT WEEK 70 35 X 35MM 638 92 TO 673 97 MASS(ES) INGUINAL REGION-RIGHT WEEK 92 20 X 30MM 680 98 TO 694 100 MASS(ES) INGUINAL REGION-RIGHT WEEK 92 30 X 30MM C92460 F9 428 62 TO 449 65 MASS(ES) VENTRAL-ABDOMINAL WEEK 62 30 X 30MM 456 66 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL WEEK 62 30 X 40MM 505 73 TO 519 75 MASS(ES) VENTRAL-ABDOMINAL WEEK 62 30 X 30MM 4015 Covance 6329-212 Appendix 2 Covance 6329-228 3M T-6316.1 Individual Clinical Observations PAGE: 233 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92460 F9 526 76 TO 547 79 MASS(ES) VENTRAL-ABDOMINAL WEEK 62 35 X 30MM 554 80 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL WEEK 62 40 X 30MM 589 85 TO 679 97 MASS(ES) VENTRAL-ABDOMINAL WEEK 62 40 X 35MM 442 64 TO 449 65 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 64 10 X 10MM 4016 APPENDIX 3 Individual Body Weight Data (g) Covance 6329-212 Covance 6329-228 3M T-6316.1 4017 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 8 - 0 PPM C92191 205 255 297 332 355 370 383 398 414 423 C92192 194 246 302 349 C92193 204 261 307 352 381 411 431 450 465 475 C92194 192 252 301 345 376 400 424 435 454 468 C92195 181 228 267 305 330 358 380 399 413 430 C92196 199 258 300 338 C92197 234 285 332 373 402 433 451 467 484 504 C92198 201 251 298 338 366 396 415 420 416 440 C92199 163 205 244 283 311 339 361 377 392 406 C92200 166 221 275 324 350 377 396 407 424 449 C92201 218 269 310 346 375 405 427 442 461 472 C92202 184 241 286 322 347 373 393 413 422 442 C92203 215 275 327 377 415 450 472 488 503 524 C92204 208 252 288 319 349 367 384 398 412 426 C92205 215 279 349 405 445 485 523 545 572 595 C92206 187 241 294 332 357 392 416 431 458 483 C92207 217 273 318 353 C92208 222 273 327 363 391 425 451 462 477 490 C92209 172 218 260 297 330 361 385 398 420 370 C92210 209 278 330 382 409 445 472 486 505 501 C92211 174 233 276 319 353 378 403 418 431 443 C92212 204 246 280 309 328 343 356 367 371 383 C92213 204 253 304 349 381 409 423 444 454 469 C92214 209 268 322 372 411 442 471 491 517 539 C92215 214 263 306 345 381 405 436 464 480 504 C92216 193 248 290 339 C92217 200 256 310 355 385 415 436 455 472 487 C92218 182 236 288 333 361 386 401 412 426 447 C92219 226 289 354 397 437 476 505 519 544 568 C92220 215 276 326 363 395 419 443 458 477 498 C92221 170 220 268 305 329 348 361 371 380 388 4018 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 8 - 0 PPM C92222 206 265 304 347 380 412 429 455 468 477 C92223 194 246 291 331 358 384 405 418 437 453 C92224 220 279 327 376 409 437 462 482 501 515 C92225 180 230 283 329 357 384 411 431 450 472 C92226 209 259 296 337 370 395 417 438 459 468 C92227 188 252 300 346 387 417 444 468 481 495 C92228 185 236 282 321 344 372 394 409 423 444 C92229 198 255 314 366 405 435 461 487 503 527 C92230 176 235 293 347 375 409 445 466 482 470 C92231 204 260 316 363 395 426 458 464 478 493 C92232 234 302 355 396 440 472 501 520 542 566 C92233 180 233 280 319 353 380 409 422 435 453 C92234 219 276 333 376 410 434 456 479 501 524 C92235 203 249 279 305 321 345 364 376 394 415 C92236 199 246 287 320 345 366 384 394 411 423 C92237 225 293 354 399 437 471 495 517 533 553 C92238 236 289 334 376 412 437 455 477 489 507 C92239 221 276 324 360 386 414 446 470 485 510 C92240 211 269 324 364 395 415 445 466 491 509 C92241 185 240 283 318 344 368 389 402 416 431 C92242 199 262 319 366 401 438 460 482 500 520 C92243 220 281 340 387 422 459 488 506 525 547 C92244 204 260 317 361 399 432 463 484 511 538 C92245 226 290 344 388 421 447 469 493 519 541 C92246 182 234 287 327 341 357 379 395 414 427 C92247 235 298 355 402 431 464 496 515 538 562 C92248 227 285 335 382 417 449 479 506 522 545 C92249 213 272 324 357 374 398 418 434 457 473 C92250 200 261 312 354 388 421 447 470 488 503 C92251 212 271 328 364 394 424 447 459 480 492 C92252 175 232 283 315 339 362 394 411 428 438 4019 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 8 - 0 PPM C92253 225 285 335 374 402 426 456 483 506 522 C92254 231 288 338 386 422 456 477 498 518 540 C92255 174 235 288 327 345 360 372 378 395 409 C92256 213 281 333 387 420 451 475 494 522 537 C92257 227 289 341 388 418 448 478 494 515 525 C92258 195 256 309 356 392 430 456 479 497 507 C92259 228 290 335 379 C92260 215 273 322 368 391 412 438 452 470 487 GROUP: MALE 9 - 1 PPM C92261 185 237 282 323 351 376 396 408 424 436 C92262 213 272 331 382 419 453 479 492 515 527 C92263 224 287 332 376 405 436 470 493 513 530 C92264 199 255 307 353 C92265 236 301 353 399 430 465 479 468 490 516 C92266 176 224 271 315 345 382 404 412 434 454 C92267 223 276 322 359 388 408 425 436 445 463 C92268 228 293 354 401 449 490 517 552 577 604 C92269 193 254 318 368 409 439 471 491 510 527 C92270 211 267 292 304 319 338 360 378 394 412 C92271 236 296 353 402 443 477 511 531 552 573 C92272 181 235 278 310 340 367 385 400 416 432 C92273 191 258 312 365 403 438 468 489 510 513 C92274 184 239 291 334 364 396 423 439 453 468 C92275 187 238 280 318 342 366 382 397 410 425 C92276 178 229 275 320 355 391 419 432 453 466 C92277 216 274 331 376 418 453 486 506 528 553 C92278 215 277 326 358 380 398 421 439 456 478 C92279 205 256 297 340 371 403 428 446 462 482 C92280 199 256 307 344 4020 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92281 209 256 300 341 380 402 424 442 460 483 C92282 190 247 300 341 376 411 438 453 470 482 C92283 200 250 293 330 360 391 419 436 447 465 C92284 216 277 319 352 C92285 208 280 341 389 433 465 500 524 545 570 C92286 219 272 317 351 389 424 443 468 484 502 C92287 198 262 314 359 395 429 456 472 489 499 C92288 221 282 332 374 412 439 466 486 503 526 C92289 195 256 307 351 384 417 446 459 480 500 C92290 207 259 304 342 370 393 407 430 444 467 C92291 197 257 304 337 365 388 406 414 427 440 C92292 183 226 263 290 317 346 370 383 397 409 C92293 206 262 303 346 377 410 431 447 469 485 C92294 214 272 315 346 375 403 424 438 464 489 C92295 188 251 308 362 402 436 467 482 507 524 C92296 180 234 275 314 342 371 398 418 437 450 C92297 171 221 267 301 332 357 372 382 397 404 C92298 222 285 343 392 439 479 513 529 552 568 C92299 180 232 269 309 338 365 383 398 407 426 C92300 214 267 319 353 C92301 218 285 331 370 393 432 460 468 493 507 C92302 220 287 352 411 456 501 538 564 587 612 C92303 196 253 301 348 380 416 441 455 471 489 C92304 215 283 338 376 407 438 465 480 502 521 C92305 225 289 343 378 409 432 461 483 510 512 C92306 213 275 325 363 392 421 436 451 465 480 C92307 165 222 268 310 344 380 405 426 446 470 C92308 169 226 274 308 333 354 375 385 406 420 C92309 213 271 315 357 373 390 413 423 445 458 C92310 212 275 336 384 425 459 496 505 531 551 C92311 213 273 323 360 384 425 448 465 483 500 4021 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92312 213 262 293 331 346 368 380 393 406 419 C92313 225 281 325 357 378 402 436 448 470 488 C92314 240 291 339 378 396 424 440 454 467 482 C92315 205 269 328 365 399 425 450 465 482 503 C92316 190 243 290 327 361 385 406 420 441 458 C92317 226 282 334 364 409 442 471 484 500 525 C92318 167 227 275 324 362 398 425 437 456 471 C92319 202 267 317 364 390 419 436 450 462 481 C92320 189 248 298 340 373 406 437 458 474 489 C92321 172 227 270 306 337 366 382 393 405 416 C92322 170 227 267 303 334 362 384 400 413 428 C92323 199 266 318 369 416 446 480 495 518 541 C92324 220 275 321 360 389 422 439 455 477 488 C92325 228 291 344 392 437 469 504 532 552 573 C92326 170 226 286 327 366 401 425 437 460 480 C92327 210 272 322 362 394 417 440 457 471 483 C92328 194 255 309 346 384 415 439 463 477 490 C92329 167 218 271 314 C92330 229 288 339 381 424 450 474 500 517 535 GROUP: FEMALE 8 - 0 PPM C92331 144 175 200 217 236 254 251 267 279 281 C92332 146 179 194 209 C92333 142 162 186 210 216 225 241 246 249 249 C92334 150 181 209 224 250 263 274 288 289 306 C92335 160 175 207 232 240 256 272 286 C92336 149 166 182 202 217 231 236 250 260 262 C92337 156 186 213 234 254 260 278 285 296 309 C92338 167 188 198 226 233 251 260 260 269 276 C92339 160 184 215 239 250 281 290 306 313 324 4022 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 8 - 0 PPM C92340 159 187 202 223 238 255 268 268 287 288 C92341 173 202 230 243 267 277 290 301 302 316 C92342 143 179 201 220 233 240 260 258 270 278 C92343 150 186 209 232 255 256 279 288 292 295 C92344 137 162 186 198 219 235 237 243 250 254 C92345 165 190 219 251 276 293 300 334 345 C92346 156 180 211 230 244 255 271 281 285 285 C92347 142 165 180 198 208 218 223 229 232 241 C92348 143 170 181 214 224 224 241 247 258 261 C92349 136 160 199 228 241 265 275 292 310 304 C92350 154 183 212 238 256 260 269 285 292 292 C92351 140 161 180 190 208 216 217 224 236 242 C92352 162 198 221 236 C92353 165 194 209 236 252 260 273 274 282 288 C92354 154 172 197 212 237 255 261 268 275 283 C92355 146 175 195 219 C92356 163 192 200 220 250 265 289 278 281 271 C92357 147 174 208 238 258 277 301 317 327 330 C92358 159 188 221 234 246 263 265 280 275 288 C92359 147 178 193 205 225 241 252 255 261 270 C92360 163 204 238 263 271 289 314 319 330 333 C92361 156 179 188 207 219 233 238 252 265 264 C92362 129 153 162 191 211 223 219 239 251 253 C92363 161 194 216 246 261 277 289 292 304 306 C92364 137 159 180 188 203 215 219 230 237 240 C92365 162 192 204 234 243 252 263 274 284 282 C92366 162 181 219 241 C92367 141 169 191 204 221 231 242 249 256 255 C92368 158 203 226 266 278 303 303 319 339 344 C92369 160 181 199 225 245 263 271 284 299 304 C92370 160 177 187 219 236 239 242 258 270 278 4023 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 8 - 0 PPM C92371 151 180 198 227 237 265 274 279 294 301 C92372 156 187 214 252 247 277 293 312 315 308 C92373 151 172 186 205 C92374 151 184 206 218 246 260 265 283 290 299 C92375 167 195 228 266 271 284 302 320 332 334 C92376 140 162 184 199 209 216 223 228 227 229 C92377 158 185 222 232 259 277 292 298 314 318 C92378 154 181 195 228 243 252 259 272 283 286 C92379 133 162 184 203 221 229 240 247 253 256 C92380 142 180 205 224 249 253 276 283 294 311 C92381 179 209 224 257 272 285 290 300 312 315 C92382 146 181 196 205 222 235 245 250 268 272 C92383 161 194 223 236 260 277 284 297 302 320 C92384 147 169 200 218 232 244 263 273 277 280 C92385 169 190 217 234 260 283 279 300 305 312 C92386 138 160 181 199 208 226 237 242 243 255 C92387 154 179 205 231 236 264 259 273 297 299 C92388 152 185 205 227 241 260 268 278 278 283 C92389 140 166 190 205 226 240 246 256 263 275 C92390 137 139 166 181 194 208 206 226 228 229 C92391 175 210 232 264 284 297 310 315 330 328 C92392 140 170 187 209 221 226 241 239 247 250 C92393 163 201 230 266 263 275 278 297 311 333 C92394 168 197 209 237 244 258 267 272 278 291 C92395 153 184 207 225 239 253 269 273 276 286 C92396 151 171 186 217 231 242 248 265 272 277 C92397 138 161 193 221 235 253 262 274 287 290 C92398 147 175 205 234 250 259 276 276 290 295 C92399 148 177 191 211 227 227 242 244 253 264 C92400 151 171 190 222 235 242 252 264 276 274 4024 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92401 164 189 214 240 250 276 294 305 309 328 C92402 147 177 205 221 C92403 163 190 214 245 251 278 285 299 308 309 C92404 149 181 207 222 250 262 277 288 287 305 C92405 159 177 207 223 234 245 257 263 265 265 C92406 150 177 187 214 217 236 242 252 260 269 C92407 150 173 209 224 247 270 283 289 302 303 C92408 145 171 199 218 233 247 256 258 272 277 C92409 162 183 205 224 231 242 253 264 267 269 C92410 169 191 219 233 247 260 263 276 281 285 C92411 147 182 213 233 248 268 274 280 293 303 C92412 150 184 206 227 245 244 268 267 278 284 C92413 171 196 211 241 253 270 274 292 301 316 C92414 174 198 220 237 261 275 284 288 305 303 C92415 155 179 210 240 250 276 280 288 303 319 C92416 144 169 194 212 220 240 247 252 250 257 C92417 149 176 208 228 252 270 272 277 284 299 C92418 154 189 210 235 248 265 278 277 284 296 C92419 159 185 213 223 249 260 252 269 276 282 C92420 156 185 211 224 254 267 279 285 297 304 C92421 165 190 214 231 243 256 265 268 277 284 C92422 149 163 196 210 224 231 246 255 260 264 C92423 147 175 201 211 238 255 262 261 278 285 C92424 157 184 195 220 234 244 243 260 267 279 C92425 144 179 203 221 228 244 255 256 263 268 C92426 126 158 181 191 211 223 230 237 248 251 C92427 155 169 198 205 220 238 241 240 252 260 C92428 172 195 208 241 256 272 272 289 298 299 C92429 141 166 186 204 217 232 243 248 258 262 C92430 157 195 217 234 244 265 280 283 285 294 C92431 158 189 213 234 244 264 274 288 288 311 4025 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92432 139 164 182 195 210 223 228 232 240 246 C92433 136 171 185 218 229 249 254 259 268 271 C92434 150 174 187 220 213 231 237 246 256 252 C92435 168 212 252 269 C92436 132 159 173 201 207 230 231 245 244 256 C92437 155 181 197 211 222 239 249 254 257 271 C92438 146 174 199 224 234 239 257 260 271 278 C92439 145 173 205 223 238 254 266 264 278 290 C92440 150 179 196 215 230 245 250 263 263 275 C92441 163 173 212 228 241 255 262 261 269 278 C92442 166 195 208 244 262 281 284 317 325 335 C92443 164 198 223 246 258 279 294 308 314 314 C92444 144 169 184 206 217 230 238 247 250 258 C92445 146 179 228 253 274 310 319 339 348 350 C92446 155 178 197 223 249 264 272 288 296 299 C92447 166 193 226 249 266 279 296 EXCLUDE 307 313 C92448 171 192 232 235 271 281 291 303 298 316 C92449 136 162 179 204 219 229 234 244 254 258 C92450 177 209 220 248 268 278 291 302 296 314 C92451 154 171 198 209 235 247 258 248 254 264 C92452 156 177 203 221 235 246 263 271 275 277 C92453 158 184 199 224 238 257 259 266 271 279 C92454 164 191 220 243 256 263 279 285 289 291 C92455 146 174 193 207 224 245 252 252 268 271 C92456 133 158 178 196 205 220 225 230 237 238 C92457 149 183 203 229 233 253 269 259 267 286 C92458 147 172 195 209 222 240 252 253 262 265 C92459 139 160 183 192 209 221 222 233 242 246 C92460 130 152 175 183 197 210 223 228 228 233 C92461 152 175 201 221 236 243 256 265 269 272 C92462 146 166 175 197 217 221 220 232 234 232 4026 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92463 170 215 233 275 C92464 128 158 180 202 211 219 230 239 240 248 C92465 146 171 205 226 245 259 264 274 286 299 C92466 158 183 198 227 241 256 266 258 274 274 C92467 156 187 201 233 C92468 153 174 203 218 232 241 240 250 262 266 C92469 129 151 181 199 214 216 231 237 239 236 C92470 160 189 205 229 4027 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 8 - 0 PPM C92191 428 431 440 425 447 462 472 498 520 525 C92193 489 503 511 513 513 526 544 559 576 583 C92194 488 506 527 542 551 565 577 609 645 656 C92195 442 456 465 475 476 489 494 524 555 558 C92197 509 525 526 537 533 551 559 588 611 608 C92198 442 C92199 418 434 446 457 454 473 487 513 540 545 C92200 460 480 485 496 481 496 512 545 569 550 C92201 483 496 500 507 504 521 528 561 579 585 C92202 452 467 472 479 478 492 491 516 541 542 C92203 540 557 573 575 591 606 621 664 701 709 C92204 432 438 445 441 454 469 472 493 508 513 C92205 605 627 636 651 661 680 690 733 748 771 C92206 501 507 520 531 544 556 568 602 629 648 C92208 502 519 530 537 543 549 556 592 626 655 C92209 311 285 335 377 418 448 466 498 522 547 C92210 521 526 539 553 557 574 571 610 636 642 C92211 457 467 476 479 486 491 497 516 537 545 C92212 398 403 415 415 423 428 434 455 471 487 C92213 482 490 496 499 514 526 537 556 577 585 C92214 557 566 579 587 597 611 618 664 682 685 C92215 518 531 544 550 566 587 599 643 670 695 C92217 502 514 526 533 548 569 573 593 627 637 C92218 465 477 499 498 509 520 526 539 545 564 C92219 580 598 612 614 635 656 672 710 733 754 C92220 520 539 549 558 574 587 595 614 640 660 C92221 397 402 417 425 431 441 440 458 469 480 C92222 479 501 524 526 531 543 553 575 600 618 C92223 465 476 486 491 503 511 520 547 570 581 C92224 526 537 555 560 572 578 588 614 632 637 C92225 487 501 516 530 538 545 547 588 616 630 C92226 480 491 503 506 511 528 534 560 579 592 4028 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 8 - 0 PPM C92227 503 524 532 544 554 562 579 609 635 649 C92228 462 480 497 505 514 521 533 559 578 595 C92229 539 552 565 574 581 590 603 636 662 689 C92230 510 532 548 558 569 581 589 607 637 658 C92231 504 520 528 521 538 539 550 565 568 590 C92232 578 602 624 631 639 644 646 678 717 760 C92233 469 479 497 501 510 518 526 550 576 591 C92234 542 561 575 583 598 606 618 661 704 725 C92235 421 431 438 445 449 456 464 470 487 496 C92236 424 431 441 452 455 453 460 470 491 499 C92237 570 584 597 606 626 625 631 661 690 708 C92238 521 540 556 565 571 585 592 626 659 681 C92239 516 529 546 552 568 578 597 622 651 675 C92240 522 539 556 570 583 599 615 648 678 691 C92241 446 455 466 473 494 503 517 533 566 586 C92242 534 552 562 569 579 588 594 622 651 677 C92243 561 571 587 602 617 633 644 682 721 738 C92244 548 566 576 578 598 608 613 643 669 689 C92245 562 576 601 618 634 643 658 707 738 754 C92246 444 459 478 486 494 501 521 548 574 583 C92247 580 602 620 631 640 662 672 713 749 754 C92248 564 578 589 599 614 629 637 670 714 723 C92249 479 487 496 504 510 527 532 560 577 598 C92250 520 546 555 572 585 604 622 665 706 723 C92251 499 509 518 529 536 547 550 574 598 614 C92252 454 467 479 501 509 520 533 547 570 584 C92253 528 548 554 568 586 599 607 642 687 707 C92254 553 567 577 596 604 614 629 660 684 691 C92255 419 425 437 442 C92256 556 573 581 598 C92257 536 545 537 535 4029 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 8 - 0 PPM C92258 527 550 560 582 C92260 501 518 527 534 GROUP: MALE 9 - 1 PPM C92261 448 458 468 472 477 480 489 519 532 548 C92262 544 553 571 580 596 617 634 647 652 657 C92263 554 570 584 592 607 627 637 669 704 705 C92265 533 553 563 570 580 593 603 628 646 647 C92266 467 483 492 502 509 514 523 540 565 576 C92267 471 496 497 507 506 512 520 539 565 562 C92268 623 642 653 665 677 697 711 766 802 822 C92269 541 556 573 589 588 605 630 679 711 747 C92270 430 444 462 469 474 488 497 528 560 562 C92271 587 601 619 632 644 653 664 713 744 753 C92272 443 459 461 472 488 494 497 528 545 539 C92273 531 545 559 569 578 588 602 639 672 690 C92274 487 506 518 520 526 538 538 558 581 590 C92275 439 452 463 464 473 483 487 508 533 544 C92276 479 491 509 513 525 531 543 568 580 594 C92277 562 582 596 606 624 635 642 680 695 719 C92278 494 506 509 518 527 547 553 572 599 611 C92279 492 513 512 531 542 551 561 589 616 636 C92281 493 504 512 515 525 529 532 549 560 576 C92282 492 502 515 523 530 528 536 565 580 592 C92283 477 489 507 512 524 533 540 561 589 610 C92285 588 612 620 628 647 670 683 730 768 811 C92286 522 544 559 571 579 593 614 657 686 706 C92287 513 524 539 544 554 558 566 595 624 634 C92288 538 554 574 583 598 606 625 679 705 716 C92289 502 517 524 528 541 544 549 581 602 611 4030 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92290 478 494 508 526 539 550 562 589 612 626 C92291 453 458 467 473 482 485 489 509 521 523 C92292 421 442 458 466 477 484 491 529 567 589 C92293 499 506 525 533 547 561 568 606 635 647 C92294 501 518 529 529 548 555 562 586 608 615 C92295 535 549 555 562 566 582 585 611 629 638 C92296 474 484 498 513 525 537 541 579 612 622 C92297 417 422 434 438 450 449 447 474 488 498 C92298 588 602 619 628 643 654 666 685 718 730 C92299 434 450 455 471 470 476 483 500 523 534 C92301 518 527 549 556 562 574 581 599 629 647 C92302 623 643 666 674 682 693 703 745 786 793 C92303 498 517 525 526 538 545 554 585 613 624 C92304 548 559 575 581 593 601 605 645 664 670 C92305 527 542 555 565 586 595 600 618 637 647 C92306 491 513 534 541 551 560 569 610 653 673 C92307 486 505 515 522 538 549 556 582 604 631 C92308 436 432 431 438 443 448 451 450 466 485 C92309 470 480 490 487 499 504 507 532 545 553 C92310 562 574 589 596 603 630 639 677 699 709 C92311 527 540 554 560 566 591 597 638 669 695 C92312 425 433 438 450 454 459 466 484 492 504 C92313 503 517 527 533 551 568 586 613 639 667 C92314 493 512 522 521 525 535 546 572 591 601 C92315 513 530 537 549 557 573 584 615 640 657 C92316 462 475 481 487 489 502 504 521 533 541 C92317 545 558 567 573 593 616 623 666 692 717 C92318 483 499 516 528 534 545 558 577 608 624 C92319 491 506 515 520 528 537 530 561 568 588 C92320 500 520 529 540 547 552 562 590 608 630 C92321 424 435 440 447 449 457 466 480 498 508 4031 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92322 434 440 452 457 464 474 473 499 521 537 C92323 556 573 585 587 595 606 623 659 673 688 C92324 505 515 529 536 553 560 571 593 614 627 C92325 580 603 622 637 C92326 489 494 520 522 C92327 495 511 516 519 C92328 506 512 524 525 C92330 555 574 584 595 GROUP: FEMALE 8 - 0 PPM C92331 272 286 295 294 282 296 302 312 325 327 C92333 255 268 265 264 271 271 276 282 295 295 C92334 304 314 329 326 330 329 333 357 368 368 C92336 258 271 272 278 267 272 281 318 338 350 C92337 306 302 316 323 317 330 337 352 375 389 C92338 277 277 292 297 290 291 299 314 324 318 C92339 322 339 353 365 366 369 390 405 425 447 C92340 292 303 307 309 313 315 330 348 357 383 C92341 315 324 333 346 341 352 365 399 416 408 C92342 270 280 288 292 291 304 309 290 298 306 C92343 293 305 319 327 336 347 353 377 399 420 C92344 253 261 260 273 269 282 276 286 306 308 C92345 341 357 361 366 372 387 395 431 487 421 C92346 296 303 301 301 305 316 316 329 331 344 C92347 250 244 249 254 266 266 262 278 289 292 C92348 259 272 270 274 286 290 314 292 290 299 C92349 324 322 322 342 333 339 353 362 375 367 C92350 292 302 310 304 306 310 315 328 332 331 C92351 241 242 254 258 257 251 264 269 276 279 C92353 286 297 297 309 307 316 320 329 333 335 4032 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 8 - 0 PPM C92354 282 294 298 300 301 311 315 321 331 350 C92356 285 293 288 303 308 303 318 320 328 335 C92357 344 359 363 369 384 393 396 422 441 473 C92358 286 291 295 299 290 304 302 304 309 312 C92359 275 278 295 304 298 300 311 315 321 325 C92360 332 348 351 339 338 343 348 361 373 388 C92361 268 275 284 291 290 295 299 288 323 314 C92362 248 262 271 275 270 275 281 289 284 301 C92363 307 322 317 331 326 339 342 359 365 369 C92364 238 245 249 254 247 259 258 262 276 290 C92365 281 291 307 302 300 311 320 334 330 345 C92367 262 269 274 272 276 287 291 300 317 315 C92368 349 360 354 373 372 368 360 389 405 418 C92369 306 319 318 321 325 332 342 361 371 384 C92370 264 286 298 295 286 299 305 322 331 359 C92371 298 300 309 316 319 320 328 347 351 385 C92372 315 313 330 330 331 339 331 350 363 368 C92374 294 314 316 316 311 312 312 339 356 378 C92375 341 354 360 366 368 384 383 409 438 448 C92376 232 240 238 245 241 247 248 251 270 269 C92377 329 324 324 331 340 342 356 359 362 357 C92378 286 297 299 305 295 308 303 337 354 368 C92379 250 260 258 264 263 265 268 288 297 298 C92380 304 324 329 335 341 341 355 346 360 381 C92381 312 324 334 333 336 339 340 371 391 404 C92382 265 276 284 287 284 296 303 311 322 333 C92383 316 327 334 338 332 338 350 353 369 373 C92384 286 296 295 295 297 302 305 316 324 325 C92385 322 323 342 338 352 360 355 380 412 447 C92386 260 268 266 273 279 282 279 292 301 324 C92387 311 316 328 335 326 344 347 377 420 435 4033 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 8 - 0 PPM C92388 280 293 303 303 309 321 322 336 344 349 C92389 273 277 280 287 281 290 293 300 313 324 C92390 241 240 259 260 256 263 265 283 301 307 C92391 327 334 332 342 346 354 358 373 382 390 C92392 247 251 263 259 257 267 261 274 272 278 C92393 313 318 321 336 345 363 345 355 361 404 C92394 294 294 294 301 307 309 305 314 328 343 C92395 285 292 291 297 296 303 308 316 330 333 C92396 276 279 283 293 C92397 283 289 292 302 C92398 288 303 315 313 C92399 255 263 259 266 C92400 276 289 299 301 GROUP: FEMALE 9 - 1 PPM C92401 328 340 344 354 353 361 361 379 407 409 C92403 320 331 332 335 340 352 355 381 423 442 C92404 299 310 317 321 317 322 328 357 372 374 C92405 269 275 276 275 279 284 280 294 299 312 C92406 274 261 282 270 277 272 286 290 297 291 C92407 304 310 324 325 311 318 325 355 378 392 C92408 274 277 284 288 284 286 288 307 317 315 C92409 270 279 284 279 284 293 289 303 313 313 C92410 287 283 299 296 292 299 300 309 328 316 C92411 302 303 314 312 310 327 337 343 333 346 C92412 275 289 288 284 297 288 297 315 310 310 C92413 310 320 315 335 357 366 379 406 451 480 C92414 314 317 319 329 330 334 335 354 372 381 C92415 314 322 330 325 341 337 344 359 372 380 C92416 265 261 262 265 271 277 273 266 297 290 4034 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92417 296 297 312 315 312 313 318 322 333 350 C92418 292 302 306 316 312 317 315 309 356 367 C92419 278 289 294 295 297 299 299 285 308 317 C92420 304 306 314 321 321 333 339 348 366 365 C92421 285 285 297 303 299 307 304 309 336 339 C92422 265 273 279 277 283 293 289 302 317 339 C92423 291 287 295 301 302 299 305 315 322 339 C92424 276 285 295 298 292 299 298 328 339 354 C92425 277 276 276 286 295 295 296 307 334 333 C92426 249 247 259 266 263 262 266 277 290 294 C92427 260 254 265 272 272 269 280 284 293 293 C92428 297 301 297 315 313 315 322 340 356 365 C92429 263 268 276 279 281 281 283 303 308 316 C92430 297 296 296 307 307 313 308 324 339 336 C92431 321 321 317 332 343 351 335 343 364 363 C92432 248 243 257 255 264 263 262 283 300 301 C92433 280 274 279 282 278 280 283 298 299 308 C92434 261 260 261 269 267 281 274 282 290 296 C92436 250 267 266 278 273 285 288 295 304 318 C92437 265 272 268 288 289 293 287 315 328 338 C92438 273 279 283 282 287 286 292 302 310 317 C92439 288 285 301 305 304 308 314 329 325 331 C92440 283 276 276 289 285 297 298 305 309 322 C92441 275 277 284 289 286 291 295 321 344 360 C92442 337 343 350 360 373 365 380 396 442 426 C92443 329 326 335 345 339 353 343 366 384 414 C92444 254 251 256 259 265 264 264 274 282 278 C92445 371 369 378 394 401 416 419 453 493 534 C92446 311 312 326 330 319 332 343 362 363 377 C92447 325 330 328 342 335 351 360 408 431 448 C92448 315 316 329 343 355 363 371 397 418 434 4035 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92449 267 267 280 282 280 292 296 313 317 319 C92450 306 312 323 336 341 346 353 382 398 412 C92451 273 283 286 290 295 291 300 341 359 366 C92452 286 293 294 294 303 305 305 321 322 330 C92453 282 296 309 312 312 317 324 334 336 354 C92454 294 300 310 308 316 317 318 321 328 344 C92455 284 296 291 288 296 299 297 309 320 323 C92456 250 253 255 253 257 265 261 271 280 284 C92457 289 295 295 302 304 303 302 322 321 323 C92458 277 273 287 290 284 289 300 336 363 388 C92459 245 245 257 252 256 264 260 282 286 289 C92460 244 250 258 265 267 273 266 291 297 308 C92461 274 284 284 285 293 296 295 312 333 336 C92462 232 242 249 253 252 258 264 268 276 286 C92464 252 251 258 263 C92465 295 295 308 314 C92466 282 286 288 298 C92468 265 278 279 277 C92469 244 250 247 250 4036 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 20 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 8 - 0 PPM C92191 547 578 578 609 616 636 652 661 675 680 C92193 605 623 624 636 633 648 649 661 657 660 C92194 686 731 737 761 774 779 796 816 834 846 C92195 584 622 630 654 661 672 C92197 629 642 653 667 673 674 677 667 667 645 C92199 569 590 609 635 656 666 C92200 596 634 656 679 701 728 753 767 789 809 C92201 606 647 642 661 668 678 693 700 715 719 C92202 559 602 624 644 647 657 664 676 685 687 C92203 740 803 828 860 888 911 943 977 995 1016 C92204 520 549 557 568 578 584 599 614 621 629 C92205 790 805 833 849 863 882 C92206 680 692 711 740 752 768 772 788 778 762 C92208 654 708 734 777 807 847 869 889 911 936 C92209 565 601 602 626 644 666 673 695 713 724 C92210 659 674 693 727 742 769 782 803 810 825 C92211 568 589 608 628 644 657 C92212 501 524 548 568 586 603 616 634 654 644 C92213 602 624 628 592 595 C92214 713 746 740 767 758 782 798 823 841 828 C92215 709 743 753 791 802 812 817 772 740 674 C92217 657 677 695 705 717 733 740 761 779 814 C92218 583 582 585 592 600 613 630 647 656 676 C92219 788 820 840 878 904 923 940 966 982 970 C92220 677 696 705 718 712 721 715 717 699 685 C92221 469 483 495 513 523 524 542 542 545 546 C92222 637 659 691 709 717 733 751 767 782 786 C92223 599 627 642 654 670 681 C92224 638 662 674 697 705 724 724 740 746 737 C92225 655 691 709 732 741 758 763 773 767 599 C92226 616 646 663 680 711 723 708 734 750 761 C92227 670 714 730 759 757 781 798 820 849 860 4037 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 21 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 8 - 0 PPM C92228 619 646 663 691 708 705 680 561 C92229 729 767 783 805 839 862 879 898 921 932 C92230 668 697 715 734 748 764 C92231 608 638 642 666 681 689 715 733 751 759 C92232 790 816 848 897 940 968 988 1009 1024 1019 C92233 608 632 641 670 679 692 692 707 715 727 C92234 742 794 829 874 877 941 954 1000 1009 1048 C92235 509 527 542 561 554 583 582 592 613 617 C92236 513 524 535 560 571 588 589 606 610 621 C92237 735 773 792 834 866 887 913 934 946 977 C92238 711 735 761 784 797 819 C92239 686 704 721 743 746 757 761 769 775 790 C92240 720 747 773 795 801 826 825 846 869 883 C92241 616 655 682 712 736 755 778 816 830 845 C92242 701 725 755 782 808 831 831 806 743 C92243 770 808 828 842 851 869 865 878 842 684 C92244 725 758 775 811 818 842 841 862 881 C92245 779 817 827 847 839 855 857 897 712 C92246 600 618 635 669 684 710 C92247 793 832 850 873 908 932 C92248 752 774 796 817 824 835 813 771 651 447 C92249 622 652 663 684 700 727 708 742 727 701 C92250 757 797 823 854 870 888 930 951 970 980 C92251 643 668 691 719 724 747 748 773 789 788 C92252 593 617 604 609 620 613 626 631 642 651 C92253 687 748 772 769 777 791 C92254 712 723 737 751 762 770 785 800 814 824 4038 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 22 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92261 569 581 599 625 638 658 680 696 715 730 C92262 675 696 705 725 741 746 755 768 767 771 C92263 733 756 767 789 814 832 840 858 873 874 C92265 679 671 704 684 722 735 753 765 757 749 C92266 601 614 638 664 687 699 717 744 755 768 C92267 566 581 599 619 634 653 C92268 872 917 944 986 1020 1046 1081 1120 1150 1170 C92269 790 827 858 888 911 940 967 989 999 1031 C92270 588 617 633 643 669 686 702 720 723 747 C92271 782 814 841 871 889 908 930 953 958 963 C92272 560 590 612 623 645 667 685 704 730 735 C92273 735 773 787 C92274 610 623 646 656 681 689 C92275 569 598 607 616 640 641 639 658 656 660 C92276 606 623 647 653 654 623 624 618 612 584 C92277 746 776 790 810 816 823 799 C92278 631 654 671 688 713 720 745 754 768 771 C92279 657 678 704 723 727 747 769 787 799 804 C92281 605 615 631 646 677 680 696 716 729 741 C92282 613 638 659 669 670 683 690 702 705 674 C92283 629 648 668 683 702 722 751 759 769 775 C92285 861 898 932 962 995 1027 1045 1087 1075 1017 C92286 726 744 757 774 793 797 C92287 662 685 704 725 746 752 C92288 745 775 808 834 855 889 C92289 637 650 672 687 706 716 646 709 734 744 C92290 648 668 678 691 693 691 611 484 C92291 534 556 571 577 583 597 590 616 629 630 C92292 630 664 695 726 741 755 711 597 490 C92293 675 692 689 C92294 645 648 655 686 701 710 725 751 752 701 4039 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 23 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92295 674 697 710 724 739 750 761 777 782 790 C92296 650 665 677 698 718 721 732 733 723 712 C92297 514 541 543 558 571 580 588 590 605 613 C92298 750 752 761 781 802 821 830 828 706 C92299 534 550 562 568 564 581 593 611 616 625 C92301 677 696 709 741 767 773 784 808 818 834 C92302 838 873 900 924 949 987 1002 1025 1098 1047 C92303 644 665 675 689 706 715 C92304 691 710 741 759 781 797 821 843 846 854 C92305 679 696 719 739 765 759 739 587 C92306 689 716 735 753 782 786 793 815 822 842 C92307 653 657 693 715 727 717 738 726 731 747 C92308 494 497 509 510 513 520 535 539 558 568 C92309 578 593 607 615 643 657 672 693 684 695 C92310 728 754 774 778 798 811 807 820 832 845 C92311 730 733 777 805 840 855 877 911 924 938 C92312 515 517 522 521 508 502 488 480 462 C92313 695 711 730 755 774 792 798 817 824 835 C92314 628 645 663 676 683 695 699 708 725 736 C92315 680 691 712 731 746 755 C92316 559 580 599 611 628 634 645 659 663 666 C92317 751 772 815 828 841 868 C92318 649 663 675 705 729 746 766 787 797 810 C92319 585 599 621 632 646 658 666 680 689 683 C92320 661 676 691 722 739 764 C92321 530 542 563 571 576 595 606 623 614 621 C92322 565 580 600 625 641 652 661 681 697 708 C92323 702 713 737 768 792 804 832 860 874 898 C92324 650 672 689 702 697 704 4040 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 24 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 8 - 0 PPM C92331 343 360 374 383 403 426 437 451 462 478 C92333 311 337 344 365 354 400 415 421 442 451 C92334 401 425 440 437 475 474 510 539 559 583 C92336 365 376 390 408 418 431 456 470 478 473 C92337 410 431 463 487 503 527 533 556 577 577 C92338 352 371 381 409 418 437 447 478 396 373 C92339 458 495 507 548 573 595 608 641 648 661 C92340 408 435 455 474 501 520 558 590 608 621 C92341 444 478 489 512 525 543 C92342 323 319 325 328 327 C92343 449 456 462 466 465 445 420 C92344 309 334 346 353 367 381 391 409 427 444 C92346 352 353 382 394 418 412 337 326 C92347 309 318 344 317 C92348 304 310 303 308 307 324 336 346 353 361 C92349 385 413 424 439 442 458 468 488 485 504 C92350 348 366 373 383 390 405 419 438 443 451 C92351 294 299 307 334 310 333 355 347 356 371 C92353 343 359 374 388 406 420 425 450 457 466 C92354 364 373 384 393 405 412 438 467 469 486 C92356 347 366 368 373 386 400 C92357 510 548 581 615 622 640 C92358 320 330 329 340 345 349 370 375 378 390 C92359 328 347 353 368 378 391 409 426 435 447 C92360 403 417 419 431 444 458 471 478 507 515 C92361 327 339 366 370 385 400 411 436 446 456 C92362 325 323 324 334 344 351 368 365 380 387 C92363 403 424 442 459 473 472 466 479 500 521 C92364 304 329 335 356 362 315 306 223 C92365 355 367 364 385 399 405 C92367 319 326 341 351 356 370 364 368 389 401 4041 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 25 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 8 - 0 PPM C92368 433 456 471 473 481 509 521 535 543 558 C92369 407 436 447 463 486 505 508 537 550 554 C92370 382 402 414 432 465 476 510 534 564 586 C92371 402 419 435 446 467 488 484 495 503 521 C92372 365 378 396 412 431 447 C92374 421 436 444 465 476 475 487 505 520 524 C92375 470 503 528 558 597 624 652 695 717 752 C92376 275 284 302 316 323 347 C92377 367 390 402 403 436 458 C92378 388 404 414 430 446 474 474 485 493 459 C92379 318 329 340 344 358 372 373 380 280 321 C92380 407 429 448 456 495 500 519 538 552 558 C92381 424 456 465 469 491 496 513 533 478 316 C92382 353 376 385 376 394 397 232 C92383 377 390 407 414 429 438 446 468 478 489 C92384 340 351 365 391 404 415 428 452 462 474 C92385 475 506 532 566 596 607 638 668 670 669 C92386 339 364 375 396 404 410 403 431 426 411 C92387 461 474 487 496 508 530 544 551 568 579 C92388 376 376 404 419 430 461 C92389 332 348 356 361 376 380 C92390 317 335 341 356 356 370 C92391 418 464 493 514 533 553 559 567 569 609 C92392 280 297 310 318 323 273 320 378 C92393 450 476 492 508 538 539 549 587 608 615 C92394 365 383 397 419 426 451 455 481 492 515 C92395 352 368 383 390 413 418 4042 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 26 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92401 442 471 485 514 536 557 588 618 633 659 C92403 462 488 501 524 466 C92404 396 421 449 462 485 490 519 530 551 564 C92405 333 347 359 369 370 388 408 414 437 431 C92406 291 314 331 329 338 358 C92407 431 421 467 489 505 516 C92408 323 341 337 351 350 376 388 407 407 422 C92409 328 350 370 386 405 409 424 435 444 455 C92410 333 351 354 359 366 380 398 405 411 407 C92411 368 378 392 390 407 429 C92412 327 345 352 374 384 385 407 418 429 439 C92413 489 499 494 498 494 489 504 550 550 580 C92414 399 424 433 446 466 472 487 509 C92415 398 415 429 449 478 487 517 539 557 546 C92416 298 323 334 347 363 373 372 384 384 401 C92417 376 402 408 423 437 463 479 520 527 537 C92418 375 395 407 419 444 456 C92419 328 332 333 335 342 339 350 361 376 365 C92420 391 403 418 437 461 464 487 517 536 538 C92421 353 367 375 384 401 382 409 439 455 473 C92422 352 369 384 384 414 426 C92423 351 366 387 395 409 428 438 458 459 469 C92424 367 385 393 397 423 427 C92425 342 379 389 394 405 422 436 460 468 473 C92426 303 311 318 320 322 338 334 343 352 356 C92427 307 332 351 392 437 461 478 543 C92428 359 386 385 403 402 411 434 444 C92429 335 342 352 373 385 393 376 383 C92430 341 361 367 385 404 402 421 438 457 461 C92431 377 408 414 433 457 475 502 502 549 554 C92432 311 328 339 349 366 387 390 411 422 408 4043 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92433 316 329 341 353 375 389 C92434 302 317 329 336 350 365 381 392 400 418 C92436 312 317 323 332 338 344 339 351 367 366 C92437 358 374 381 382 355 C92438 333 335 347 355 368 369 C92439 333 348 345 370 368 376 386 387 395 409 C92440 330 336 354 356 396 391 395 418 421 419 C92441 381 394 407 442 470 486 C92442 468 496 528 560 586 606 634 656 593 C92443 444 469 486 517 555 573 589 620 660 660 C92444 297 305 316 330 342 361 353 385 375 394 C92445 573 602 645 676 716 732 793 843 875 878 C92446 399 410 443 480 493 517 550 594 610 606 C92447 467 487 500 506 545 563 592 637 659 653 C92448 447 468 465 496 506 522 533 545 476 394 C92449 310 329 333 346 370 386 C92450 434 451 458 471 471 487 505 524 536 538 C92451 380 393 420 436 447 466 477 503 521 537 C92452 342 340 357 370 381 396 395 408 425 437 C92453 370 390 404 424 439 456 486 492 512 527 C92454 351 371 389 411 446 450 476 507 481 485 C92455 339 353 363 383 396 420 427 453 468 463 C92456 289 302 316 335 354 383 408 427 445 450 C92457 342 361 365 375 384 393 398 420 421 430 C92458 411 435 452 469 486 504 521 534 350 C92459 504 322 305 321 336 351 372 377 388 400 C92460 317 341 339 346 372 384 397 409 416 416 C92461 360 376 393 400 425 446 477 414 C92462 279 295 326 358 393 400 435 449 465 485 4044 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 28 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: C92191 C92193 C92194 C92197 C92200 C92201 C92202 C92203 C92204 C92206 C92208 C92209 C92210 C92212 C92214 C92215 C92217 C92218 C92219 C92220 C92221 C92222 C92224 C92226 C92227 C92229 C92231 C92232 C92233 C92234 C92235 C92236 MALE 8 - 0 PPM 695 694 670 679 855 843 649 633 823 682 726 715 621 607 1041 1015 655 644 657 916 938 737 737 843 839 669 666 786 736 610 907 1052 686 677 1009 1022 626 483 545 536 792 768 753 757 766 790 900 906 950 932 770 772 1063 1060 729 743 1063 1074 628 606 637 635 705 689 872 616 741 585 1020 603 967 724 851 670 705 693 1061 531 800 748 800 933 839 803 1078 759 953 625 649 733 714 890 634 792 565 1023 1003 728 861 699 607 697 1058 522 825 767 748 950 695 835 1080 773 685 649 648 733 714 902 608 793 1002 1014 734 867 709 689 474 811 758 611 965 832 1054 768 630 540 767 725 907 619 800 957 1043 726 906 698 665 348 842 789 976 849 1016 763 622 807 739 922 607 857 1079 642 771 654 614 866 817 844 866 858 734 636 828 703 854 576 730 1049 515 592 483 843 795 708 837 525 696 565 580 1078 565 417 856 843 401 4045 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 29 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 8 - 0 PPM C92237 987 987 1008 1036 1035 957 879 661 C92239 794 787 804 830 832 839 801 650 504 C92240 893 879 856 C92241 856 853 856 878 857 673 C92250 1005 1016 1062 1063 1028 C92251 812 825 874 C92252 659 655 680 688 708 707 734 718 712 C92254 839 796 731 483 GROUP: MALE 9 - 1 PPM C92261 748 748 782 791 782 800 828 800 823 C92262 783 770 769 764 674 614 532 506 C92263 886 881 884 897 885 905 894 840 791 C92265 724 669 C92266 770 776 797 822 826 841 865 838 836 C92268 1201 1191 1201 1181 1161 1029 841 C92269 1037 C92270 761 766 802 830 854 876 886 818 797 C92271 946 928 900 874 811 C92272 733 722 705 680 C92275 656 654 647 661 652 634 593 483 C92276 371 C92278 774 763 785 786 806 827 836 820 836 C92279 819 808 763 665 542 C92281 750 731 759 764 766 757 749 714 711 C92282 652 628 638 647 639 638 641 614 613 C92283 781 781 814 839 835 850 831 772 712 C92285 829 C92289 745 741 773 769 752 735 701 627 559 C92291 634 635 657 677 676 695 4046 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 30 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92294 C92295 C92296 C92297 C92299 C92301 C92302 C92304 C92306 C92307 C92308 C92309 C92310 C92311 C92313 C92314 C92316 C92318 C92319 C92321 C92322 C92323 657 809 642 628 635 845 1059 873 853 750 573 712 826 936 844 732 679 817 690 632 732 921 700 808 641 641 837 1058 866 844 707 571 709 938 852 743 690 821 676 626 727 938 755 823 650 647 876 1072 871 861 707 570 728 959 856 759 690 825 702 629 751 950 653 849 678 653 892 1069 899 887 678 564 738 981 876 763 633 825 702 625 760 948 548 864 686 665 1046 896 886 626 559 740 994 899 673 654 816 693 625 771 955 504 888 700 677 929 896 914 612 557 749 1001 918 550 640 818 723 637 793 970 377 891 705 693 942 592 594 743 1039 899 559 849 708 645 811 985 859 687 666 888 522 600 678 1037 528 842 693 618 788 939 861 677 608 555 594 1044 825 690 606 799 850 GROUP: FEMALE 8 - 0 PPM C92331 514 525 525 495 473 C92333 465 485 502 536 549 564 598 597 608 C92334 590 608 650 677 672 699 710 703 705 C92336 486 473 420 C92337 546 509 445 C92339 681 701 714 757 767 743 4047 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 31 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 8 - 0 PPM C92340 614 573 C92344 450 472 470 493 479 487 481 421 C92348 374 378 387 399 408 427 438 432 464 C92349 523 514 C92350 452 472 472 459 434 401 C92351 382 386 385 408 426 435 455 443 452 C92353 479 497 512 547 542 428 C92354 496 520 541 560 557 540 518 473 414 C92358 402 404 407 440 437 444 444 410 381 C92359 448 463 468 472 443 326 C92360 518 544 533 538 583 599 614 604 630 C92361 470 481 498 505 525 571 634 672 C92362 384 396 395 401 393 382 383 371 340 C92363 547 572 587 C92367 408 423 435 453 459 464 478 481 498 C92368 576 582 595 649 660 660 640 529 C92369 553 554 558 575 562 560 572 590 658 C92370 622 653 658 692 695 679 655 C92371 540 545 548 562 521 C92374 522 542 560 589 598 607 626 604 609 C92375 762 763 696 562 C92378 315 C92380 467 434 339 C92383 502 509 527 549 564 574 599 584 596 C92384 480 464 488 473 C92385 678 661 647 536 504 429 C92386 417 418 403 401 402 387 375 350 337 C92387 416 C92391 676 703 698 667 623 521 C92393 630 638 594 616 545 509 C92394 516 415 372 382 4048 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 32 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92401 688 685 640 565 474 C92404 572 578 519 C92405 454 470 480 487 493 497 391 C92408 433 441 432 440 466 461 456 471 C92409 475 487 496 514 531 534 429 C92410 423 428 442 455 466 479 497 496 487 C92412 448 448 465 466 483 496 505 494 497 C92413 619 629 635 666 678 631 625 519 C92415 510 501 516 551 531 C92416 407 423 429 439 445 448 432 439 452 C92417 569 602 611 530 403 275 C92419 389 387 402 415 428 434 449 446 442 C92420 572 C92421 483 496 503 524 544 565 577 541 466 C92423 482 492 500 511 533 541 572 584 611 C92425 494 516 525 565 593 606 634 C92426 346 343 352 361 362 350 378 384 414 C92430 497 482 501 519 533 552 555 542 542 C92431 564 562 584 611 619 637 651 644 640 C92432 356 345 C92434 431 430 436 454 492 C92436 381 382 381 415 428 446 C92439 424 413 426 433 435 450 461 460 474 C92440 448 440 C92443 680 707 744 783 795 808 802 718 620 C92444 414 417 431 419 431 447 450 453 457 C92445 877 888 903 938 950 950 C92446 619 603 433 C92447 615 518 436 392 338 C92450 531 488 405 357 281 C92451 557 566 574 597 604 549 526 534 547 4049 Covance 6329-212 Appendix 3 Covance 6329-228 3M T-6316.1 Individual Body Weight Data (g) PAGE: 33 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92452 437 462 465 488 481 504 511 511 519 C92453 545 580 598 615 608 508 445 C92454 456 389 299 C92455 482 498 520 566 580 609 633 524 C92456 468 473 490 513 515 544 551 537 561 C92457 448 447 413 338 C92459 419 430 437 446 429 400 375 C92460 436 444 447 421 379 360 319 C92462 495 513 527 540 538 457 366 4050 APPENDIX 4 Covance 6329-212 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) Individual Test Material Consumption Data (mg/kg/day) 4051 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 8 - 0 PPM C92191 C92192 C92193 C92194 C92195 C92196 C92197 C92198 C92199 C92200 C92201 C92202 C92203 C92204 C92205 C92206 C92207 C92208 C92209 C92210 C92211 C92212 C92213 C92214 C92215 C92216 C92217 C92218 C92219 C92220 C92221 159 162 166 161 156 149 167 173 169 166 161 168 181 NOT TAKEN 163 166 173 180 180 179 188 183 177 180 174 174 185 177 176 173 173 180 176 187 162 201 168 203 177 180 176 174 171 172 173 168 170 NOT TAKEN 162 166 179 184 179 167 180 176 177 168 161 166 174 167 177 177 168 149 176 156 135 143 155 158 164 163 163 162 165 166 155 167 183 183 182 186 185 186 205 198 158 161 168 177 187 179 173 182 182 174 165 163 163 163 162 169 175 164 166 166 168 178 193 195 197 200 206 203 203 198 145 145 154 159 154 158 157 156 158 158 174 187 194 205 205 212 213 203 210 203 157 169 174 172 184 183 184 188 193 199 181 180 182 NOT TAKEN 158 168 172 171 178 187 178 171 172 170 146 147 156 170 166 168 180 175 148 18 189 199 212 205 211 213 222 213 211 189 153 SPILLED 166 166 166 168 170 163 163 169 144 145 146 148 147 147 152 152 155 157 161 182 182 186 186 187 195 SPILLED 197 SPILLED 179 189 198 197 201 196 205 205 207 204 164 167 177 184 192 200 197 188 198 193 154 152 164 NOT TAKEN 176 184 192 188 SPILLED SPILLED 195 185 190 199 152 162 169 170 175 160 161 162 168 177 186 207 211 217 218 217 223 227 226 227 181 202 181 199 191 183 188 192 196 208 146 153 159 158 158 152 156 147 154 161 4052 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 8 - 0 PPM C92222 188 C92223 168 C92224 178 C92225 154 C92226 150 C92227 170 C92228 159 C92229 167 C92230 158 C92231 172 C92232 197 C92233 159 C92234 182 C92235 154 C92236 158 C92237 184 C92238 171 C92239 168 C92240 181 C92241 149 C92242 181 C92243 176 C92244 166 C92245 183 C92246 149 C92247 186 C92248 169 C92249 172 C92250 174 C92251 172 C92252 163 175 187 191 195 193 197 191 195 189 172 177 180 200 180 199 199 201 190 178 187 195 200 199 202 195 191 190 161 171 175 179 183 180 177 181 183 153 161 167 169 171 178 170 169 173 169 177 184 181 177 178 178 178 178 162 164 168 165 169 171 168 175 177 183 194 204 206 201 209 200 203 204 171 194 199 221 236 234 SPILLED SPILLED SPILLED 176 183 179 179 182 177 171 170 177 201 203 223 221 223 220 230 237 245 162 168 174 176 184 186 186 197 188 195 211 208 212 202 204 211 210 217 152 149 149 156 160 165 171 173 177 157 160 164 166 163 168 166 169 159 186 194 194 194 204 205 202 197 201 167 174 175 174 173 180 173 179 182 170 176 176 178 190 186 189 195 189 187 193 194 191 206 224 215 199 210 155 167 171 179 179 171 166 169 169 183 191 186 189 184 189 190 184 187 185 191 186 188 195 197 199 207 206 172 185 189 193 200 208 203 210 202 193 198 195 196 197 210 217 211 214 157 164 154 156 157 163 178 185 190 192 198 194 194 210 206 207 205 210 174 189 193 201 202 205 199 200 200 180 180 179 205 SPILLED 206 203 196 180 178 186 194 195 195 201 188 192 199 182 185 183 180 182 182 186 183 170 172 SPILLED SPILLED SPILLED SPILLED 192 222 SPILLED 226 4053 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 8 - 0 PPM C92253 183 177 185 189 182 200 205 210 197 184 C92254 180 185 203 203 204 204 215 210 221 214 C92255 174 182 185 172 171 172 176 187 195 188 C92256 167 175 191 184 193 187 196 199 197 208 C92257 170 177 185 183 185 187 187 192 183 178 C92258 180 187 185 195 192 189 193 188 182 200 C92259 177 175 187 NOT TAKEN C92260 176 219 221 SPILLED 223 225 221 223 225 220 GROUP: MALE 9 - 1 PPM C92261 144 152 160 164 159 159 159 164 162 166 C92262 175 188 204 200 194 199 204 207 211 203 C92263 180 177 181 180 183 190 194 198 196 199 C92264 162 174 183 NOT TAKEN C92265 185 192 204 202 205 215 176 SPILLED 221 SPILLED C92266 141 149 163 158 168 165 162 162 166 175 C92267 157 149 157 152 149 150 151 148 158 155 C92268 185 204 209 230 235 236 250 244 244 228 C92269 177 197 211 206 214 212 230 SPILLED 254 SPILLED C92270 SPILLED 217 229 SPILLED SPILLED SPILLED 209 232 192 218 C92271 176 183 195 197 204 SPILLED 209 211 208 218 C92272 149 149 156 156 159 159 164 158 164 167 C92273 176 183 198 200 206 214 214 199 233 211 C92274 154 155 169 167 170 171 173 166 176 189 C92275 146 145 153 153 157 153 162 160 163 170 C92276 143 155 SPILLED 180 180 187 182 185 175 177 C92277 171 190 198 205 214 212 212 209 210 204 C92278 168 177 179 167 170 172 182 179 184 182 C92279 173 SPILLED 213 191 197 205 202 194 191 194 C92280 162 175 188 NOT TAKEN 4054 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92281 150 160 167 176 174 184 176 179 182 185 C92282 159 165 166 177 178 176 182 178 179 184 C92283 162 164 173 181 183 186 194 181 200 195 C92284 188 176 177 NOT TAKEN C92285 179 193 200 201 199 210 208 213 224 226 C92286 158 161 175 182 187 184 187 183 184 186 C92287 162 164 172 176 179 183 180 178 173 178 C92288 182 184 186 191 187 191 192 189 199 198 C92289 166 171 186 195 200 203 205 208 202 199 C92290 159 167 173 169 174 171 184 183 193 188 C92291 161 159 160 162 158 158 154 153 151 147 C92292 139 139 147 150 156 160 163 159 162 168 C92293 173 185 83 187 SPILLED 197 198 192 193 200 C92294 159 156 154 159 164 170 178 191 208 200 C92295 175 214 209 202 200 197 197 204 198 199 C92296 145 151 157 155 157 157 161 160 165 173 C92297 140 147 155 157 155 154 153 161 165 172 C92298 175 179 190 197 208 210 207 200 202 199 C92299 154 153 162 171 170 179 183 SPILLED SPILLED 183 C92300 163 176 181 NOT TAKEN C92301 191 188 199 192 200 198 180 188 193 192 C92302 179 194 204 205 213 213 215 224 221 217 C92303 162 167 182 182 190 190 184 178 183 179 C92304 181 187 196 196 195 198 200 197 207 207 C92305 192 200 197 200 202 214 208 192 204 194 C92306 180 192 193 183 198 184 185 193 190 195 C92307 164 160 173 177 188 189 196 195 194 191 C92308 140 148 155 156 155 159 165 163 168 171 C92309 180 184 187 180 177 183 185 208 191 186 C92310 174 185 195 204 204 207 209 223 201 205 C92311 186 185 192 190 194 200 194 201 194 206 4055 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92312 154 156 165 163 166 167 178 167 172 173 C92313 172 171 173 167 175 182 194 183 195 194 C92314 167 171 181 176 187 176 184 180 181 181 C92315 175 190 190 187 208 207 217 204 207 210 C92316 157 197 SPILLED 190 SPILLED 178 165 165 162 159 C92317 167 174 176 185 190 189 190 191 192 194 C92318 157 168 180 196 SPILLED 186 193 212 188 188 C92319 177 194 SPILLED 194 198 185 196 176 183 183 C92320 172 185 189 192 197 198 204 196 200 201 C92321 155 164 161 168 170 163 174 163 173 168 C92322 162 160 156 173 165 162 163 161 163 161 C92323 188 190 204 208 203 203 203 208 206 206 C92324 168 174 180 186 199 185 201 192 190 194 C92325 185 194 206 209 207 214 228 214 215 219 C92326 161 177 179 181 180 180 178 180 186 179 C92327 179 192 195 200 204 195 190 182 178 183 C92328 164 175 185 197 SPILLED SPILLED SPILLED SPILLED 215 205 C92329 146 164 176 NOT TAKEN C92330 174 180 191 204 195 199 201 196 199 207 GROUP: FEMALE 8 - 0 PPM C92331 C92332 C92333 C92334 C92335 C92336 C92337 C92338 C92339 SPILLED 129 156 SPILLED 122 109 159 134 126 SPILLED 117 SPILLED SPILLED 126 108 SPILLED SPILLED SPILLED SPILLED 119 SPILLED 135 127 112 130 179 173 SPILLED NOT TAKEN 161 161 124 121 141 SPILLED 158 SPILLED SPILLED 149 139 124 SPILLED SPILLED 173 SPILLED SPILLED 154 142 124 144 SPILLED 159 SPILLED 134 154 141 130 140 235 161 SPILLED 149 166 NOT TAKEN 126 152 190 156 157 SPILLED 164 122 154 219 160 138 144 SPILLED 122 142 SPILLED 150 4056 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 8 - 0 PPM C92340 C92341 C92342 C92343 C92344 C92345 C92346 C92347 C92348 C92349 C92350 C92351 C92352 C92353 C92354 C92355 C92356 C92357 C92358 C92359 C92360 C92361 C92362 C92363 C92364 C92365 C92366 C92367 C92368 C92369 C92370 122 SPILLED SPILLED 139 123 112 SPILLED 108 SPILLED 131 132 111 156 SPILLED 118 SPILLED SPILLED 127 128 112 132 110 SPILLED 125 108 139 121 116 153 128 113 145 140 132 133 SPILLED 131 128 108 127 135 129 109 SPILLED 140 116 SPILLED SPILLED 142 136 108 137 SPILLED 108 131 112 SPILLED 131 114 169 130 117 137 142 123 137 SPILLED 149 SPILLED 112 SPILLED 206 140 108 SPILLED 167 123 127 165 150 126 115 143 121 121 143 107 171 145 112 166 134 129 145 SPILLED SPILLED 145 SPILLED 162 128 118 SPILLED 140 141 115 NOT TAKEN 141 138 NOT TAKEN 139 161 125 129 135 SPILLED 142 145 113 141 NOT TAKEN 121 SPILLED 141 127 132 150 121 137 SPILLED 155 SPILLED 121 SPILLED 149 138 110 138 136 136 SPILLED 133 125 139 119 122 137 113 SPILLED 116 SPILLED 145 118 136 171 128 139 200 153 137 113 156 142 143 105 SPILLED 131 145 186 128 127 148 127 SPILLED 141 113 190 124 151 149 119 140 144 124 141 SPILLED 160 141 113 149 152 150 110 148 142 132 156 137 131 137 132 130 147 117 159 122 164 158 135 148 148 118 142 SPILLED 161 132 123 125 159 137 117 SPILLED SPILLED 118 148 123 139 141 124 SPILLED SPILLED 109 140 127 165 152 128 132 138 127 127 SPILLED 146 127 125 121 144 126 112 SPILLED 133 SPILLED 143 127 148 139 123 115 133 113 SPILLED 121 149 152 128 132 145 121 SPILLED SPILLED 151 136 126 147 162 139 113 SPILLED 150 142 150 128 180 136 131 SPILLED 137 111 SPILLED 133 154 159 121 4057 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 8 - 0 PPM C92371 C92372 C92373 C92374 C92375 C92376 C92377 C92378 C92379 C92380 C92381 C92382 C92383 C92384 C92385 C92386 C92387 C92388 C92389 C92390 C92391 C92392 C92393 C92394 C92395 C92396 C92397 C92398 C92399 C92400 147 134 143 162 155 SPILLED SPILLED 153 SPILLED 162 SPILLED 135 142 NOT TAKEN 121 118 124 134 133 135 143 150 147 148 SPILLED 127 114 119 135 127 132 133 129 143 129 129 143 139 141 SPILLED 115 120 122 120 124 128 127 145 136 SPILLED 143 155 SPILLED 155 129 116 112 119 121 130 131 131 155 141 SPILLED 165 124 129 128 SPILLED 129 132 156 144 158 141 144 SPILLED 142 121 120 SPILLED 137 149 SPILLED SPILLED 126 SPILLED 173 127 151 125 129 130 SPILLED SPILLED 166 SPILLED 137 131 133 141 148 156 SPILLED 157 152 126 111 139 SPILLED 155 134 137 SPILLED SPILLED 139 141 136 SPILLED SPILLED 125 141 130 SPILLED 124 150 SPILLED 145 118 122 129 SPILLED 136 SPILLED SPILLED 138 157 134 119 115 115 124 107 121 126 135 133 130 137 SPILLED 133 150 114 144 142 122 146 162 119 139 137 SPILLED 152 SPILLED 152 132 SPILLED 149 135 128 132 154 148 SPILLED 168 120 135 135 159 146 162 112 137 148 123 146 SPILLED 124 143 135 149 123 136 151 131 145 146 125 146 139 130 SPILLED 139 130 123 144 130 151 131 153 105 146 137 119 137 SPILLED 138 143 129 148 122 154 SPILLED 135 193 139 126 138 SPILLED 142 SPILLED 131 138 117 137 124 135 135 150 103 127 136 118 146 169 126 145 131 148 126 148 173 133 SPILLED 130 119 156 152 133 SPILLED 127 128 129 131 122 152 138 155 116 143 141 109 136 SPILLED 125 144 SPILLED SPILLED 131 160 SPILLED 132 160 SPILLED 117 126 156 139 SPILLED 130 132 113 144 4058 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92401 C92402 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 C92412 C92413 C92414 C92415 C92416 C92417 C92418 C92419 C92420 C92421 C92422 C92423 C92424 C92425 C92426 C92427 C92428 C92429 C92430 C92431 132 123 120 131 148 104 123 112 158 120 129 120 SPILLED 117 136 151 SPILLED 122 SPILLED SPILLED 123 107 SPILLED SPILLED 139 112 106 121 SPILLED 132 123 125 132 128 135 123 139 141 117 SPILLED 121 SPILLED SPILLED SPILLED 111 139 SPILLED SPILLED 124 SPILLED SPILLED 125 120 SPILLED 160 134 127 131 123 SPILLED 131 125 138 136 145 136 125 120 139 120 131 120 201 174 139 116 154 144 141 131 136 SPILLED 124 114 208 171 141 127 108 138 124 164 129 141 NOT TAKEN 137 155 128 114 154 128 129 119 SPILLED SPILLED 144 141 161 132 166 138 157 SPILLED 124 116 SPILLED SPILLED 140 121 122 136 131 138 131 150 148 148 127 SPILLED 147 127 139 126 SPILLED SPILLED 142 137 160 133 158 131 SPILLED SPILLED 128 122 SPILLED SPILLED 140 115 SPILLED 135 131 153 134 147 150 144 162 150 149 122 156 117 SPILLED 143 139 132 153 125 133 125 SPILLED SPILLED 149 125 145 SPILLED 132 137 SPILLED 137 143 136 140 151 144 150 149 133 143 123 143 125 139 169 146 139 145 122 137 117 SPILLED 160 127 121 159 SPILLED 129 121 148 139 125 148 139 144 132 140 SPILLED 130 138 128 SPILLED 130 SPILLED SPILLED 143 143 160 117 143 117 SPILLED SPILLED SPILLED 119 SPILLED 165 140 125 211 SPILLED SPILLED 135 144 149 132 149 117 134 131 123 130 119 SPILLED SPILLED 160 143 157 117 137 120 159 163 SPILLED 121 SPILLED 156 129 SPILLED 125 133 124 131 151 144 148 146 144 133 136 113 135 127 SPILLED 120 SPILLED 148 149 124 135 116 SPILLED SPILLED SPILLED 122 SPILLED SPILLED 131 113 126 131 126 132 147 4059 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92432 C92433 C92434 C92435 C92436 C92437 C92438 C92439 C92440 C92441 C92442 C92443 C92444 C92445 C92446 C92447 C92448 C92449 C92450 C92451 C92452 C92453 C92454 C92455 C92456 C92457 C92458 C92459 C92460 C92461 C92462 108 SPILLED 125 144 108 113 124 114 SPILLED 112 139 138 SPILLED 124 132 125 151 SPILLED 138 112 132 124 148 120 SPILLED 128 122 114 124 140 116 107 SPILLED 109 153 100 105 120 118 163 131 140 135 128 144 129 137 151 121 130 117 SPILLED 116 150 117 SPILLED 122 129 118 SPILLED 148 SPILLED 111 178 127 147 114 112 128 119 159 126 153 144 141 150 152 178 143 126 141 114 SPILLED 134 151 125 SPILLED 133 134 117 SPILLED 129 124 115 205 113 NOT TAKEN 116 111 131 127 153 130 162 147 SPILLED 156 150 155 160 135 141 132 SPILLED 131 158 130 198 129 135 120 123 138 126 117 SPILLED 120 122 122 133 127 133 135 158 152 138 163 147 152 154 127 157 134 SPILLED 138 148 133 SPILLED 141 135 122 138 SPILLED 122 110 SPILLED 123 118 125 130 124 131 129 159 163 SPILLED 162 SPILLED 151 144 SPILLED 139 131 SPILLED SPILLED 164 126 SPILLED 132 145 120 139 144 SPILLED 109 SPILLED 120 121 118 125 127 129 126 175 159 150 161 157 142 144 165 142 113 SPILLED SPILLED SPILLED SPILLED SPILLED 118 124 120 129 130 137 120 SPILLED 128 112 120 127 138 130 141 163 160 SPILLED 155 140 131 143 SPILLED 138 110 SPILLED 138 142 129 SPILLED SPILLED 134 124 147 SPILLED 127 109 129 119 115 116 122 121 136 115 156 135 SPILLED 150 132 145 142 139 131 122 163 137 141 123 133 140 131 122 197 SPILLED 131 112 SPILLED 125 112 125 116 122 127 120 161 155 SPILLED 165 155 SPILLED 143 SPILLED 140 130 156 145 145 137 SPILLED 128 145 121 SPILLED 146 122 4060 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92463 143 150 156 NOT TAKEN C92464 114 115 117 128 122 127 134 117 121 128 C92465 127 135 150 152 150 160 143 149 144 SPILLED C92466 132 130 135 137 138 140 132 145 143 135 C92467 127 119 149 NOT TAKEN C92468 133 122 129 119 126 125 132 132 131 240 C92469 SPILLED 130 143 131 129 150 155 125 122 121 C92470 134 143 138 NOT TAKEN 4061 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: C92191 C92193 C92194 C92195 C92197 C92198 C92199 C92200 C92201 C92202 C92203 C92204 C92205 C92206 C92208 C92209 C92210 C92211 C92212 C92213 C92214 C92215 C92217 C92218 C92219 C92220 C92221 C92222 C92223 C92224 C92225 C92226 MALE 8 - 0 166 179 195 172 176 NOT TAKEN 167 196 175 166 201 154 206 186 173 109 194 166 150 186 201 189 190 178 221 200 153 202 195 196 181 172 PPM 171 182 210 168 165 167 195 179 165 207 159 205 187 179 183 201 182 153 195 197 193 195 190 223 196 159 205 195 200 187 174 173 NOT TAKEN 164 165 165 170 174 171 190 NOT TAKEN 186 197 183 188 SPILLED 189 195 NOT TAKEN 190 195 191 204 189 204 173 NOT TAKEN 170 174 164 174 173 178 179 NOT TAKEN 176 180 175 183 186 173 181 NOT TAKEN 184 180 170 174 170 164 190 NOT TAKEN 176 189 186 174 167 194 176 NOT TAKEN 181 188 179 174 190 180 165 NOT TAKEN 164 166 164 174 173 171 208 NOT TAKEN 204 212 212 222 217 223 163 SPILLED 161 161 157 156 155 153 204 208 202 209 204 192 205 209 188 186 183 191 183 189 SPILLED 201 175 168 170 178 178 185 194 163 213 204 200 202 188 197 219 201 220 210 214 SPILLED 202 199 204 204 179 SPILLED 163 159 162 166 165 166 153 151 146 148 149 148 156 156 195 216 210 SPILLED SPILLED SPILLED SPILLED 223 203 205 199 201 203 205 207 198 199 203 201 206 191 186 199 180 194 200 201 201 189 199 198 197 184 187 178 184 174 162 184 185 227 228 227 231 227 218 220 227 202 198 197 201 188 207 204 211 164 161 158 152 154 153 164 149 211 203 204 205 198 217 218 212 204 202 195 197 194 210 212 195 207 199 195 197 196 203 201 180 191 188 183 181 187 195 194 193 168 169 168 170 168 179 184 181 4062 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 8 - 0 PPM C92227 188 183 192 184 186 195 191 C92228 184 189 183 182 182 185 176 C92229 200 197 200 194 195 202 191 C92230 219 229 234 229 225 SPILLED 226 C92231 175 177 175 168 172 173 158 C92232 SPILLED SPILLED 234 221 230 247 244 C92233 188 198 196 186 186 186 189 C92234 216 219 221 220 211 219 208 C92235 168 169 165 162 163 166 154 C92236 162 164 162 163 148 153 149 C92237 201 202 206 203 196 202 197 C92238 185 190 188 188 192 187 192 C92239 191 192 188 197 197 204 198 C92240 206 210 213 203 214 210 203 C92241 167 180 173 179 169 170 158 C92242 187 190 195 197 188 190 188 C92243 200 206 210 213 208 207 210 C92244 206 205 206 210 208 202 196 C92245 216 214 217 219 222 226 215 C92246 190 204 210 200 193 187 191 C92247 211 221 217 209 215 214 202 C92248 196 213 198 207 199 203 194 C92249 178 182 178 181 190 191 173 C92250 200 201 207 208 203 213 196 C92251 173 178 179 174 173 179 173 C92252 SPILLED 240 217 225 193 SPILLED 180 C92253 195 191 200 195 193 196 185 C92254 212 209 214 211 214 211 203 C92255 180 181 186 NOT TAKEN C92256 200 208 212 NOT TAKEN C92257 174 161 155 NOT TAKEN 196 206 210 187 184 192 209 211 221 229 SPILLED 210 148 166 165 248 247 239 202 199 197 222 213 200 162 166 156 154 149 152 205 206 207 193 203 196 206 218 195 205 205 209 173 178 184 190 201 188 206 205 215 203 204 210 220 221 225 180 178 175 209 205 221 198 203 202 175 187 195 202 204 206 176 177 186 180 183 176 200 202 163 208 206 202 4063 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 8 - 0 PPM C92258 205 211 218 NOT TAKEN C92260 219 239 222 NOT TAKEN GROUP: MALE 9 - 1 PPM C92261 158 167 166 NOT TAKEN 167 171 177 174 182 190 C92262 200 207 207 NOT TAKEN 206 221 203 196 213 200 C92263 193 198 200 NOT TAKEN 210 213 203 205 202 204 C92265 SPILLED 234 232 NOT TAKEN 236 226 210 200 226 227 C92266 175 180 180 NOT TAKEN 179 177 146 175 185 182 C92267 160 163 166 NOT TAKEN 161 158 158 164 166 156 C92268 225 229 238 NOT TAKEN SPILLED 243 236 237 246 249 C92269 SPILLED SPILLED SPILLED NOT TAKEN SPILLED 202 236 167 SPILLED 249 C92270 174 214 184 NOT TAKEN 201 185 173 176 193 185 C92271 202 221 227 NOT TAKEN 213 214 221 221 228 221 C92272 165 168 179 175 171 175 172 186 SPILLED 176 C92273 203 204 215 197 207 218 SPILLED 228 SPILLED 234 C92274 185 187 184 168 171 174 171 173 173 180 C92275 166 171 166 162 165 159 162 162 167 171 C92276 177 183 186 188 183 184 180 181 193 188 C92277 207 209 215 208 204 201 195 185 206 209 C92278 173 180 181 173 179 177 165 170 172 175 C92279 189 191 199 192 194 192 178 195 191 188 C92281 174 171 183 174 183 173 169 167 178 183 C92282 185 182 185 182 180 179 186 197 199 208 C92283 SPILLED 196 202 188 194 189 173 182 189 176 C92285 225 217 225 225 228 226 214 212 224 233 C92286 184 193 193 182 182 197 186 186 191 181 C92287 175 183 188 181 186 186 184 187 182 198 C92288 196 205 201 197 199 208 207 205 214 204 C92289 196 193 197 191 194 192 186 183 189 196 4064 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92290 189 189 202 197 201 195 174 182 179 185 C92291 150 155 158 163 163 153 152 162 166 163 C92292 174 178 182 179 176 176 181 192 189 198 C92293 196 219 210 207 199 204 198 207 212 213 C92294 195 192 196 184 202 195 171 180 180 189 C92295 201 199 190 187 206 197 175 193 191 195 C92296 170 178 180 179 179 178 183 188 191 197 C92297 167 175 171 180 174 166 175 183 187 194 C92298 202 208 221 211 215 210 188 217 204 207 C92299 187 195 196 178 185 184 SPILLED 182 SPILLED 182 C92301 192 202 203 203 209 203 197 215 204 210 C92302 220 227 228 213 213 218 212 226 205 241 C92303 176 182 186 183 183 189 185 194 199 197 C92304 193 208 205 205 202 198 208 206 214 222 C92305 190 202 211 212 214 213 184 186 196 192 C92306 196 207 210 205 210 211 195 205 191 203 C92307 194 203 200 199 199 200 188 204 211 211 C92308 163 169 176 157 157 154 SPILLED 166 164 184 C92309 184 189 180 173 177 173 172 174 181 184 C92310 198 207 215 207 220 222 201 192 193 190 C92311 193 201 202 193 201 205 203 210 196 214 C92312 165 170 174 171 172 173 177 176 176 167 C92313 191 197 204 201 210 207 202 196 208 209 C92314 182 189 191 179 188 192 183 184 189 195 C92315 213 211 205 210 216 219 206 201 192 199 C92316 160 171 160 171 165 167 149 154 158 154 C92317 196 190 192 196 204 198 191 192 201 199 C92318 193 202 204 198 194 211 197 199 194 193 C92319 188 200 196 194 190 169 202 195 218 196 C92320 207 206 196 206 201 203 196 194 196 198 C92321 169 174 170 166 175 173 163 174 173 171 4065 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92322 171 171 168 163 163 163 165 170 177 170 C92323 209 215 221 209 211 214 207 209 222 210 C92324 188 203 195 201 202 209 200 201 208 206 C92325 215 223 223 NOT TAKEN C92326 180 201 191 NOT TAKEN C92327 182 184 178 NOT TAKEN C92328 SPILLED 200 195 NOT TAKEN C92330 201 203 205 NOT TAKEN GROUP: FEMALE 8 - 0 PPM C92331 151 SPILLED 132 NOT TAKEN 167 SPILLED 159 155 165 195 C92333 136 141 144 NOT TAKEN 122 128 141 SPILLED SPILLED 212 C92334 141 146 146 NOT TAKEN 151 134 142 140 156 155 C92336 127 114 123 NOT TAKEN 119 129 153 149 159 136 C92337 142 147 142 NOT TAKEN 146 148 145 165 177 171 C92338 180 SPILLED 194 NOT TAKEN SPILLED SPILLED 157 128 170 146 C92339 160 171 160 NOT TAKEN 153 170 151 156 173 177 C92340 143 130 138 NOT TAKEN 133 141 125 130 165 155 C92341 133 SPILLED 155 NOT TAKEN 140 155 150 137 162 177 C92342 121 123 133 NOT TAKEN 134 137 139 127 152 138 C92343 140 160 154 149 157 157 140 147 175 171 C92344 SPILLED SPILLED 136 125 135 131 129 159 131 122 C92345 148 134 143 150 146 139 177 186 95 C92346 126 115 124 132 122 123 128 123 137 126 C92347 120 SPILLED 132 140 120 123 116 130 134 133 C92348 SPILLED 124 133 129 123 SPILLED 114 114 121 119 C92349 150 133 157 132 146 151 134 145 148 157 C92350 131 131 121 126 126 126 125 130 133 132 C92351 108 111 109 105 97 110 110 109 119 117 C92353 SPILLED 125 194 164 128 SPILLED 133 134 141 145 4066 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 8 - 0 PPM C92354 145 140 136 137 142 140 124 147 168 151 C92356 SPILLED 116 SPILLED SPILLED 130 SPILLED 128 132 141 147 C92357 142 140 149 151 145 142 136 137 166 170 C92358 124 118 127 117 122 119 122 133 135 137 C92359 SPILLED 195 159 SPILLED 144 SPILLED 195 193 202 SPILLED C92360 133 130 114 125 130 129 134 131 148 134 C92361 125 128 136 128 125 SPILLED 124 134 126 125 C92362 SPILLED SPILLED 118 121 118 SPILLED SPILLED 123 134 136 C92363 SPILLED 124 141 145 135 SPILLED 130 170 157 163 C92364 106 104 109 103 110 107 108 118 133 135 C92365 SPILLED 161 SPILLED 148 148 SPILLED 134 129 SPILLED 136 C92367 114 125 123 131 126 131 124 140 129 129 C92368 150 125 148 135 126 119 132 145 153 147 C92369 151 139 142 144 143 150 148 152 SPILLED 161 C92370 140 130 124 125 141 125 130 139 160 170 C92371 109 113 114 119 112 113 116 301 142 125 C92372 125 136 136 126 135 121 134 142 157 146 C92374 135 126 127 125 108 58 151 135 157 177 C92375 153 150 151 SPILLED 156 155 143 168 164 173 C92376 111 103 107 107 108 107 115 123 127 127 C92377 118 117 139 148 143 135 134 120 122 118 C92378 136 128 136 129 131 121 151 155 166 158 C92379 113 107 110 113 107 112 127 132 139 140 C92380 140 138 136 141 138 137 160 153 159 162 C92381 150 SPILLED 150 SPILLED SPILLED 147 176 168 198 185 C92382 127 121 122 116 125 129 119 128 142 148 C92383 140 146 135 134 142 156 137 139 146 139 C92384 125 120 121 125 122 120 116 117 118 120 C92385 135 145 142 SPILLED 141 133 136 150 167 161 C92386 119 122 119 126 118 118 117 130 160 149 C92387 131 136 145 137 141 146 130 162 163 150 4067 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 8 - 0 PPM C92388 SPILLED SPILLED 132 SPILLED SPILLED 148 SPILLED 154 143 144 C92389 120 126 132 126 122 128 121 134 SPILLED 129 C92390 SPILLED SPILLED SPILLED SPILLED 132 SPILLED 133 SPILLED SPILLED 137 C92391 SPILLED SPILLED SPILLED 137 136 SPILLED SPILLED 196 SPILLED 160 C92392 121 121 118 109 122 114 115 113 119 117 C92393 130 123 149 153 166 125 137 137 198 203 C92394 135 127 133 127 118 126 117 129 144 146 C92395 128 144 128 134 130 132 200 142 145 134 C92396 SPILLED SPILLED 167 NOT TAKEN C92397 118 129 133 NOT TAKEN C92398 133 131 132 NOT TAKEN C92399 107 111 112 NOT TAKEN C92400 141 125 133 NOT TAKEN GROUP: FEMALE 9 - 1 PPM C92401 145 147 149 NOT TAKEN 144 151 146 153 163 167 C92403 129 162 146 NOT TAKEN 136 139 143 152 167 155 C92404 134 142 143 NOT TAKEN 139 148 149 155 145 160 C92405 SPILLED 122 134 NOT TAKEN 140 121 115 121 146 140 C92406 118 SPILLED 120 NOT TAKEN 128 132 135 148 148 137 C92407 127 131 130 NOT TAKEN 131 130 148 144 166 162 C92408 116 119 117 NOT TAKEN 113 119 117 119 130 119 C92409 118 124 123 NOT TAKEN 132 120 120 136 139 129 C92410 113 125 125 NOT TAKEN 120 117 114 125 118 131 C92411 SPILLED SPILLED 210 NOT TAKEN 153 SPILLED SPILLED 189 184 173 C92412 SPILLED SPILLED 146 SPILLED 126 SPILLED SPILLED 122 133 161 C92413 133 132 148 181 172 181 170 183 189 164 C92414 126 139 138 137 129 131 141 140 152 145 C92415 133 145 127 146 139 133 148 134 151 154 C92416 100 114 116 SPILLED SPILLED 125 127 124 128 131 4068 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92417 129 137 131 122 123 129 115 129 144 136 C92418 116 125 131 117 119 114 132 127 127 132 C92419 SPILLED 151 SPILLED 192 SPILLED SPILLED SPILLED SPILLED SPILLED 158 C92420 139 SPILLED 173 177 189 SPILLED SPILLED SPILLED 234 221 C92421 SPILLED 134 132 124 126 117 119 137 145 149 C92422 110 114 117 120 116 110 115 126 141 137 C92423 SPILLED 148 172 SPILLED SPILLED SPILLED 157 SPILLED 172 165 C92424 SPILLED 167 161 SPILLED 180 SPILLED 238 SPILLED 213 218 C92425 114 120 128 127 120 120 121 137 125 141 C92426 SPILLED 120 131 SPILLED SPILLED SPILLED 226 SPILLED 154 143 C92427 SPILLED 145 SPILLED SPILLED SPILLED SPILLED SPILLED 122 121 143 C92428 125 131 129 117 122 132 130 136 135 110 C92429 129 132 122 SPILLED 120 127 129 131 142 174 C92430 124 140 143 128 126 128 130 132 135 132 C92431 123 126 145 146 130 121 127 147 155 153 C92432 98 112 108 116 106 112 121 129 133 128 C92433 106 115 109 SPILLED 108 119 158 115 124 126 C92434 115 115 125 132 125 136 129 122 138 132 C92436 118 116 123 114 118 127 115 121 150 121 C92437 127 126 138 127 118 120 136 147 179 168 C92438 112 119 123 120 115 121 125 132 137 141 C92439 114 125 122 126 128 137 145 130 126 119 C92440 116 123 140 123 138 132 119 132 143 132 C92441 118 122 120 SPILLED 113 118 146 148 150 155 C92442 133 135 153 160 145 143 146 177 159 163 C92443 143 135 154 137 154 136 150 144 159 165 C92444 SPILLED SPILLED SPILLED SPILLED SPILLED SPILLED SPILLED 167 SPILLED 179 C92445 139 147 164 155 155 151 153 168 169 176 C92446 134 SPILLED 145 116 138 144 142 137 157 168 C92447 149 SPILLED 152 144 162 SPILLED 186 188 208 203 C92448 133 147 160 166 157 162 156 158 167 170 4069 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92449 SPILLED SPILLED 131 161 159 142 130 134 142 138 C92450 127 144 163 142 141 154 150 154 164 161 C92451 126 140 134 139 131 148 166 155 160 159 C92452 152 161 166 169 171 168 SPILLED 151 158 SPILLED C92453 154 149 148 151 146 142 128 121 144 139 C92454 141 161 150 156 147 143 120 121 145 140 C92455 153 125 129 140 130 134 120 116 130 132 C92456 129 SPILLED SPILLED 124 125 136 153 112 122 118 C92457 125 134 139 134 130 132 142 128 133 156 C92458 132 138 139 117 128 142 146 149 160 159 C92459 104 121 128 124 137 130 134 133 122 124 C92460 SPILLED SPILLED 192 SPILLED 239 SPILLED SPILLED 207 SPILLED 180 C92461 144 147 157 SPILLED 158 SPILLED 149 163 175 172 C92462 150 133 155 132 139 141 142 135 146 125 C92464 123 134 133 NOT TAKEN C92465 139 151 141 NOT TAKEN C92466 SPILLED 144 158 NOT TAKEN C92468 SPILLED 132 123 NOT TAKEN C92469 119 114 121 NOT TAKEN 4070 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 20 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 8 - 0 PPM C92191 172 168 171 172 C92193 SPILLED 198 202 199 C92194 187 184 194 182 C92195 174 168 165 160 C92197 175 167 162 165 C92199 168 161 163 156 C92200 180 186 169 182 C92201 177 172 164 165 C92202 174 172 167 160 C92203 231 217 SPILLED 227 C92204 150 156 146 148 C92205 190 192 196 195 C92206 188 184 185 187 C92208 194 192 199 201 C92209 197 188 198 193 C92210 200 193 199 214 C92211 168 158 206 165 C92212 157 157 155 153 C92213 203 206 111 129 C92214 192 168 188 188 C92215 193 198 206 196 C92217 195 191 181 179 C92218 173 168 161 162 C92219 219 221 215 215 C92220 200 200 188 182 C92221 157 157 157 155 C92222 208 209 198 197 C92223 195 182 193 197 C92224 200 198 193 178 C92225 191 191 185 183 C92226 186 175 184 191 C92227 208 203 199 182 170 165 172 171 172 172 245 SPILLED SPILLED 197 185 209 185 185 202 193 201 205 166 168 160 146 160 158 146 157 174 183 204 198 196 191 174 175 183 185 187 184 166 164 176 169 163 SPILLED 238 230 240 244 SPILLED 266 153 158 159 174 181 178 201 181 180 197 190 178 89 199 193 202 212 204 212 197 187 194 197 189 191 214 215 SPILLED 231 235 212 168 157 153 165 174 166 175 198 193 199 197 173 167 193 173 134 145 117 151 191 178 192 186 206 204 170 165 168 174 191 194 222 214 223 228 224 238 185 SPILLED 175 180 169 128 151 152 150 156 160 158 199 190 198 193 204 207 187 194 185 194 196 187 202 180 176 188 186 52 191 168 185 198 203 214 190 193 205 209 216 229 4071 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 21 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 8 - 0 PPM C92228 181 177 186 178 171 149 80 NOT TAKEN C92229 214 201 194 213 214 208 198 214 227 228 C92230 201 201 202 195 194 C92231 172 170 159 168 177 176 186 193 185 192 C92232 227 255 240 245 237 231 240 242 231 SPILLED C92233 194 188 190 186 184 184 188 195 194 215 C92234 220 217 229 162 235 211 237 239 244 243 C92235 159 162 164 139 162 148 141 159 157 157 C92236 148 151 154 146 149 148 159 171 157 171 C92237 202 193 207 206 196 225 234 241 253 244 C92238 190 194 200 188 194 C92239 187 197 189 173 189 183 184 190 202 218 C92240 203 209 198 193 210 188 195 214 208 213 C92241 187 192 174 178 183 189 200 192 190 206 C92242 180 202 191 189 193 187 192 144 C92243 210 209 202 196 201 180 219 189 95 C92244 210 204 203 210 210 185 190 209 C92245 212 208 197 159 207 SPILLED 217 22 NOT TAKEN C92246 182 180 172 177 186 C92247 212 204 206 205 213 C92248 194 202 193 189 196 192 170 119 46 C92249 183 179 183 182 187 149 201 159 169 NOT TAKEN C92250 197 207 189 196 198 203 212 216 217 209 C92251 178 190 180 173 181 175 189 188 197 206 C92252 167 160 159 178 163 155 162 167 176 180 C92253 198 194 158 168 192 C92254 184 207 204 208 203 207 212 218 226 221 4072 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 22 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92261 192 185 183 179 170 181 187 190 187 184 C92262 199 195 188 196 187 190 186 194 193 188 C92263 200 195 197 199 194 188 208 209 202 210 C92265 214 221 237 217 200 199 199 201 201 189 C92266 161 169 170 172 172 174 179 187 190 190 C92267 160 160 160 159 154 C92268 238 241 237 246 237 237 243 261 253 261 C92269 243 241 235 228 238 232 244 248 259 245 C92270 183 176 181 182 176 176 179 179 194 193 C92271 229 223 213 217 213 216 225 235 219 218 C92272 SPILLED 170 168 166 165 166 186 183 193 177 C92273 210 210 C92274 173 176 149 170 170 C92275 175 164 169 173 162 160 170 172 174 169 C92276 184 188 182 174 181 173 173 182 171 35 C92277 206 201 199 200 196 162 NOT TAKEN C92278 175 171 164 177 165 175 177 181 171 193 C92279 189 189 176 174 180 184 197 198 196 210 C92281 176 173 167 177 169 160 176 189 193 193 C92282 203 191 199 190 177 182 186 197 158 159 C92283 180 179 175 174 181 183 177 183 189 187 C92285 232 225 230 234 225 229 230 220 233 85 C92286 182 179 172 176 171 C92287 184 186 182 191 174 C92288 205 208 204 212 211 C92289 184 183 177 178 179 173 207 195 192 182 C92290 178 173 164 160 148 72 60 C92291 166 163 155 162 160 143 167 174 177 177 C92292 197 196 189 181 184 128 93 77 C92293 210 202 C92294 180 172 189 171 163 174 181 171 125 134 4073 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 23 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92295 191 177 186 196 201 194 202 176 184 202 C92296 188 186 185 183 167 165 166 172 162 158 C92297 SPILLED 174 179 174 174 161 162 179 183 187 C92298 189 192 210 201 195 189 196 76 C92299 180 181 173 164 SPILLED SPILLED SPILLED 180 190 193 C92301 205 190 191 194 195 200 206 209 203 213 C92302 224 218 232 220 219 210 234 164 238 237 C92303 SPILLED 186 190 184 182 C92304 209 211 200 207 202 199 204 221 210 223 C92305 194 188 185 186 190 193 161 C92306 192 193 187 200 194 188 195 197 202 230 C92307 189 202 197 198 185 211 201 217 224 227 C92308 183 190 158 173 163 208 SPILLED SPILLED 215 SPILLED C92309 176 172 172 179 166 165 170 182 186 190 C92310 197 196 190 187 190 170 194 210 213 123 C92311 SPILLED 208 198 204 203 206 229 226 220 228 C92312 162 153 150 146 142 147 143 137 C92313 208 207 201 205 197 195 197 217 215 230 C92314 184 187 181 175 178 169 172 189 186 180 C92315 192 192 188 181 195 C92316 155 149 150 147 154 149 151 157 154 168 C92317 202 212 205 199 191 C92318 182 177 181 177 173 182 181 189 195 197 C92319 197 187 181 192 173 176 185 187 SPILLED 192 C92320 200 192 199 193 199 C92321 166 165 158 149 166 150 158 167 167 176 C92322 168 162 166 161 157 156 167 178 172 180 C92323 196 196 194 197 185 194 200 216 224 221 C92324 210 196 203 226 206 4074 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 24 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 8 - 0 PPM C92331 168 157 159 160 204 171 SPILLED SPILLED 203 221 C92333 196 169 164 137 178 SPILLED SPILLED 174 SPILLED 193 C92334 154 131 129 135 146 158 146 152 197 185 C92336 125 155 127 149 136 157 160 164 150 161 C92337 160 173 167 157 163 158 181 179 188 148 C92338 139 SPILLED 137 137 141 136 152 74 127 C92339 173 173 169 164 165 212 186 186 188 204 C92340 156 155 167 161 SPILLED 201 188 170 176 142 C92341 170 157 170 161 156 C92342 125 135 124 131 C92343 152 140 147 151 133 138 C92344 133 126 125 130 135 127 142 153 156 149 C92346 117 145 145 148 102 63 SPILLED C92347 117 140 95 NOT TAKEN C92348 119 113 125 126 122 136 125 147 139 148 C92349 165 152 148 137 155 140 147 166 154 196 C92350 127 132 126 127 133 137 134 134 141 148 C92351 110 117 121 107 100 132 111 104 120 128 C92353 149 136 137 141 141 136 136 164 200 170 C92354 143 140 145 146 125 142 153 141 165 167 C92356 144 130 145 155 165 C92357 177 173 173 171 180 C92358 135 132 135 136 139 140 132 127 162 160 C92359 167 172 151 SPILLED 156 165 SPILLED 177 177 181 C92360 133 128 136 139 142 135 128 163 149 168 C92361 131 145 136 133 136 138 148 156 158 163 C92362 127 119 122 134 130 143 116 149 200 183 C92363 SPILLED 151 146 153 126 141 153 174 178 186 C92364 135 122 142 110 71 62 54 C92365 122 114 139 130 123 C92367 129 119 124 132 139 124 111 141 130 136 4075 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 25 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 8 - 0 PPM C92368 146 128 140 123 165 162 153 160 164 171 C92369 164 157 156 166 167 166 179 181 156 169 C92370 153 149 159 184 159 162 173 183 191 213 C92371 126 127 141 134 138 123 145 151 162 155 C92372 135 143 150 155 160 C92374 156 147 155 146 155 158 161 158 146 142 C92375 164 172 168 186 191 185 180 201 207 206 C92376 129 133 126 131 165 C92377 132 132 97 110 147 NOT TAKEN C92378 150 148 158 145 167 143 143 153 128 4 C92379 142 139 136 138 160 149 149 SPILLED 82 C92380 150 153 133 197 139 151 193 168 167 75 C92381 168 167 161 172 171 157 153 82 6 C92382 151 148 134 145 150 2 C92383 139 133 131 118 145 144 151 158 163 168 C92384 123 126 139 130 140 142 154 153 171 152 C92385 153 157 160 158 147 167 164 170 181 192 C92386 149 145 151 141 142 137 144 153 144 146 C92387 142 141 145 151 163 150 153 171 170 SPILLED C92388 125 150 143 133 157 C92389 124 121 140 118 125 C92390 125 130 133 126 123 C92391 177 162 169 159 176 163 175 162 223 234 C92392 125 118 115 109 39 142 181 NOT TAKEN C92393 181 191 167 186 179 183 192 202 188 208 C92394 141 142 151 143 163 132 158 163 165 169 C92395 143 130 140 135 145 4076 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 26 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92401 154 152 168 162 173 157 183 172 195 190 C92403 167 139 151 92 C92404 158 164 143 152 147 149 163 158 181 166 C92405 139 126 136 131 146 143 133 150 141 166 C92406 150 148 124 136 147 C92407 145 154 171 138 172 C92408 135 120 135 122 137 125 125 126 143 138 C92409 151 138 148 140 151 141 157 156 148 179 C92410 135 127 121 115 130 133 132 137 137 141 C92411 168 163 159 215 175 C92412 119 131 147 134 SPILLED 142 SPILLED 147 159 150 C92413 166 140 152 156 168 120 176 204 162 212 C92414 143 139 134 144 132 149 152 C92415 153 133 151 156 144 159 172 167 160 133 C92416 134 123 130 121 129 108 124 121 147 137 C92417 148 121 146 140 147 132 151 149 168 177 C92418 125 124 132 131 131 C92419 129 133 124 117 116 122 133 206 123 153 C92420 188 192 175 193 SPILLED 183 178 191 162 168 C92421 157 144 138 146 120 139 171 152 174 167 C92422 132 135 123 138 SPILLED C92423 SPILLED 183 152 168 SPILLED 150 154 143 168 165 C92424 SPILLED 209 SPILLED 245 SPILLED C92425 149 130 130 130 133 135 148 130 144 143 C92426 133 128 137 130 141 122 121 123 127 121 C92427 SPILLED SPILLED 174 172 263 163 SPILLED C92428 146 128 129 134 115 128 80 C92429 135 139 131 126 120 79 114 C92430 139 131 144 141 127 138 139 141 159 156 C92431 166 133 159 141 127 163 144 175 185 149 C92432 136 131 134 133 149 138 142 151 146 77 4077 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92433 C92434 C92436 C92437 C92438 C92439 C92440 C92441 C92442 C92443 C92444 C92445 C92446 C92447 C92448 C92449 C92450 C92451 C92452 C92453 C92454 C92455 C92456 C92457 C92458 C92459 C92460 C92461 C92462 131 134 127 166 122 SPILLED 138 SPILLED 161 160 146 178 139 188 147 146 153 SPILLED 173 143 146 131 131 144 159 137 147 168 151 125 130 120 153 133 114 131 162 165 141 SPILLED 181 166 190 158 148 147 148 160 135 146 134 130 130 150 120 SPILLED 163 151 120 126 132 144 134 128 128 178 173 171 SPILLED 187 148 197 169 150 153 138 165 134 152 135 130 125 144 129 130 137 181 130 129 130 85 128 117 168 170 172 170 SPILLED 185 152 196 157 160 138 133 156 143 165 127 134 123 153 129 140 170 172 128 139 127 SPILLED 108 123 179 179 153 148 205 160 199 134 187 154 SPILLED 163 132 148 134 139 131 147 134 129 185 143 139 115 127 132 165 180 SPILLED 199 179 SPILLED 158 145 SPILLED SPILLED 141 161 124 140 SPILLED 163 139 128 176 159 149 127 111 220 170 192 SPILLED 214 162 211 154 149 195 SPILLED 163 160 134 128 131 138 127 137 68 136 156 SPILLED 125 130 80 185 151 215 169 198 61 158 206 SPILLED 141 122 146 155 124 2 134 137 167 148 154 132 148 136 141 NOT TAKEN 172 145 200 174 203 78 170 216 213 177 155 128 144 157 146 149 174 147 167 201 145 193 172 148 157 222 SPILLED 176 132 148 152 148 151 155 165 4078 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 28 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 8 - 0 PPM C92191 173 151 157 155 152 169 191 NOT TAKEN C92193 SPILLED SPILLED 203 192 174 182 191 176 C92194 183 192 187 188 182 187 70 C92197 139 132 141 140 141 134 173 126 C92200 37 C92201 194 195 192 185 211 C92202 155 141 126 C92203 252 212 216 220 173 136 SPILLED SPILLED C92204 176 98 C92206 NOT TAKEN C92208 208 196 188 200 188 220 227 251 C92209 191 191 162 177 167 143 141 180 C92210 218 187 210 217 207 98 C92212 168 158 152 164 153 145 132 NOT TAKEN C92214 167 136 5 C92215 NOT TAKEN C92217 220 C92218 180 180 172 168 140 149 59 117 C92219 240 225 172 C92220 85 C92221 158 146 139 132 92 C92222 179 195 189 171 200 219 212 216 C92224 197 167 181 172 204 211 SPILLED C92226 211 184 145 102 C92227 230 208 199 202 216 83 84 C92229 215 160 164 C92231 195 175 172 169 170 174 195 188 C92232 251 240 214 220 206 91 NOT TAKEN C92233 212 199 192 195 182 168 29 SPILLED C92234 250 93 8 C92235 147 131 152 118 123 156 C92236 178 151 149 54 4079 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 29 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 8 - 0 PPM C92237 236 C92239 204 C92240 214 C92241 186 C92250 230 C92251 208 C92252 175 C92254 207 220 219 220 SPILLED 127 SPILLED 184 194 199 186 143 77 SPILLED 174 185 190 123 29 227 183 162 179 NOT TAKEN 167 166 161 157 215 187 153 249 69 GROUP: MALE 9 - 1 PPM C92261 183 183 177 173 181 189 186 211 C92262 179 181 186 184 213 SPILLED SPILLED C92263 202 186 197 186 196 181 174 176 C92265 130 NOT TAKEN C92266 194 179 178 166 163 168 185 176 C92268 245 203 186 195 110 69 NOT TAKEN C92270 197 191 186 195 190 191 190 204 C92271 226 SPILLED 168 153 C92272 174 158 185 C92275 170 163 171 170 154 144 115 NOT TAKEN C92278 176 170 178 200 184 172 194 202 C92279 184 129 125 SPILLED C92281 183 174 162 169 160 157 179 182 C92282 160 156 152 152 137 146 157 152 C92283 207 186 187 178 176 158 169 154 C92285 NOT TAKEN C92289 193 177 169 165 156 143 144 135 C92291 167 175 173 173 178 NOT TAKEN C92294 244 204 113 71 73 20 C92295 202 181 184 184 189 166 187 193 4080 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 30 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92297 183 169 169 168 154 157 174 162 C92299 190 SPILLED 168 173 172 169 145 95 C92301 206 194 178 C92302 252 218 202 213 109 NOT TAKEN C92304 203 206 202 184 183 NOT TAKEN C92306 201 183 196 187 187 185 190 C92307 210 182 160 152 148 143 141 C92308 207 SPILLED SPILLED SPILLED 182 154 161 101 C92309 191 183 175 179 173 170 166 130 C92311 200 213 211 204 200 219 249 233 C92313 221 211 216 224 215 170 C92314 192 184 181 132 SPILLED C92316 170 147 SPILLED 148 121 80 114 C92318 201 185 182 186 161 203 199 183 C92319 159 177 164 158 176 179 197 169 C92321 172 177 148 158 147 150 159 150 C92322 176 153 162 165 176 162 181 194 C92323 186 201 197 187 199 199 196 147 GROUP: FEMALE 8 - 0 PPM C92331 SPILLED 141 90 144 NOT TAKEN C92333 SPILLED SPILLED SPILLED SPILLED SPILLED 151 190 162 C92334 SPILLED SPILLED 157 143 146 184 156 142 C92336 158 90 C92337 160 95 C92339 205 176 181 175 176 C92340 131 C92344 163 135 137 131 129 134 88 C92348 171 147 144 156 116 170 128 169 C92349 150 4081 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 31 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 8 - 0 PPM C92350 C92351 C92353 C92354 C92358 C92359 C92360 C92361 C92362 C92363 C92367 C92368 C92369 C92370 C92371 C92374 C92375 C92380 C92383 C92384 C92385 C92386 C92391 C92393 C92394 139 132 172 SPILLED 176 SPILLED 155 162 SPILLED 166 SPILLED 163 172 196 151 158 171 109 166 136 161 161 209 206 82 136 115 140 SPILLED 155 148 110 144 158 116 130 161 143 152 133 157 89 SPILLED 150 162 135 SPILLED 157 178 126 104 114 145 SPILLED 151 158 131 114 156 125 174 158 174 123 151 32 157 126 24 132 119 185 SPILLED 78 100 129 118 141 54 181 130 145 126 82 SPILLED 174 142 132 SPILLED 151 154 127 123 177 146 164 165 180 174 118 138 212 151 152 NOT TAKEN 113 53 142 128 134 51 118 115 145 NOT TAKEN 132 124 142 176 135 130 105 165 151 147 131 128 112 153 SPILLED 132 147 82 202 NOT TAKEN 157 SPILLED 92 101 156 82 148 180 157 159 158 163 NOT TAKEN 136 135 136 GROUP: FEMALE 9 - 1 PPM C92401 SPILLED 127 76 61 NOT TAKEN C92404 166 60 C92405 161 152 135 144 126 18 4082 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 32 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92408 144 130 128 146 C92409 162 SPILLED 165 162 C92410 144 128 135 139 C92412 128 140 114 139 C92413 185 165 177 166 C92415 139 156 164 111 C92416 144 136 128 129 C92417 188 165 49 25 C92419 133 141 124 137 C92421 159 144 151 166 C92423 158 144 149 165 C92425 179 153 161 159 C92426 135 134 136 142 C92430 143 133 165 149 C92431 166 159 159 163 C92432 143 C92434 173 156 SPILLED 157 C92436 139 120 164 157 C92439 126 128 120 117 C92440 142 NOT TAKEN C92443 207 177 192 178 C92444 SPILLED 162 SPILLED 133 C92445 199 206 199 187 C92446 156 3 C92447 63 80 82 83 C92450 128 48 89 72 C92451 226 SPILLED 203 197 C92452 184 SPILLED 155 177 C92453 183 155 148 128 C92454 110 73 C92455 153 151 149 157 131 155 SPILLED NOT TAKEN 130 18 142 139 140 125 139 145 140 147 117 132 119 123 93 136 156 85 130 126 153 139 143 134 123 97 159 163 192 199 144 132 NOT TAKEN 142 140 185 128 150 154 148 159 172 165 156 176 149 NOT TAKEN 117 117 132 134 179 167 134 111 130 162 SPILLED SPILLED 160 NOT TAKEN 52 SPILLED SPILLED SPILLED 166 198 SPILLED SPILLED 75 63 153 160 58 4083 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Food Consumption Data (g) PAGE: 33 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92456 SPILLED 131 135 127 140 138 133 157 C92457 159 84 89 C92459 167 153 142 126 89 86 C92460 147 134 80 107 58 SPILLED C92462 182 173 148 131 59 37 4084 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92261 C92262 C92263 C92264 C92265 C92266 C92267 C92268 C92269 C92270 C92271 C92272 C92273 C92274 C92275 C92276 C92277 C92278 C92279 C92280 C92281 C92282 C92283 C92284 C92285 C92286 C92287 C92288 C92289 C92290 C92291 0.097 0.103 0.101 0.102 0.098 0.101 0.090 0.101 0.113 SPILLED 0.095 0.102 0.112 0.104 0.098 0.100 0.100 0.098 0.107 0.102 0.092 0.104 0.103 0.109 0.105 0.092 0.101 0.103 0.105 0.097 0.101 0.084 0.089 0.082 0.088 0.084 0.086 0.071 0.090 0.098 0.111 0.081 0.083 0.092 0.084 0.080 0.088 0.090 0.084 SPILLED 0.089 0.082 0.086 0.086 0.084 0.089 0.078 0.081 0.086 0.087 0.085 0.081 0.076 0.082 0.073 0.079 0.078 0.079 0.066 0.079 0.088 0.110 0.074 0.076 0.084 0.077 0.073 SPILLED 0.080 0.075 0.096 0.083 0.074 0.074 0.079 0.075 0.078 0.075 0.073 0.075 0.081 0.077 0.071 0.070 0.071 0.066 NOT TAKEN 0.070 0.068 0.058 0.077 0.076 SPILLED 0.067 0.069 0.074 0.068 0.066 0.076 0.074 0.065 0.077 NOT TAKEN 0.070 0.071 0.075 NOT TAKEN 0.070 0.070 0.067 0.069 0.076 0.068 0.066 0.062 0.064 0.062 NOT TAKEN 0.065 0.066 0.053 0.072 0.072 SPILLED 0.063 0.064 0.070 0.064 0.063 0.069 0.070 0.062 0.073 NOT TAKEN 0.064 0.065 0.070 NOT TAKEN 0.063 0.066 0.062 0.063 0.071 0.065 0.060 0.059 0.061 0.060 NOT TAKEN 0.065 0.060 0.051 0.067 0.067 SPILLED SPILLED 0.060 0.067 0.060 0.058 0.066 0.065 0.060 0.070 NOT TAKEN 0.064 0.059 0.066 NOT TAKEN 0.062 0.061 0.059 0.060 0.067 0.061 0.057 0.057 0.060 0.058 NOT TAKEN 0.053 0.057 0.050 0.067 0.068 0.081 0.057 0.060 0.064 0.057 0.059 0.061 0.061 0.060 0.066 NOT TAKEN 0.058 0.058 0.065 NOT TAKEN 0.058 0.059 0.055 0.058 0.065 0.063 0.054 0.056 0.059 0.056 NOT TAKEN SPILLED 0.055 0.048 0.062 SPILLED 0.086 0.056 0.055 0.057 0.053 0.057 0.060 0.058 0.057 0.061 NOT TAKEN 0.057 0.055 0.059 NOT TAKEN 0.057 0.055 0.053 0.055 0.063 0.060 0.052 0.054 0.058 0.054 NOT TAKEN 0.063 0.053 0.050 0.059 0.070 0.068 0.053 0.055 0.065 0.055 0.056 0.054 0.056 0.056 0.058 NOT TAKEN 0.055 0.054 0.063 NOT TAKEN 0.057 0.053 0.050 0.055 0.059 0.061 0.050 0.054 0.054 0.052 NOT TAKEN SPILLED 0.054 0.047 0.053 SPILLED 0.074 0.054 0.055 0.058 0.057 0.056 0.054 0.052 0.053 0.057 NOT TAKEN 0.054 0.054 0.059 NOT TAKEN 0.056 0.052 0.050 0.053 0.057 0.057 0.047 4085 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92292 C92293 C92294 C92295 C92296 C92297 C92298 C92299 C92300 C92301 C92302 C92303 C92304 C92305 C92306 C92307 C92308 C92309 C92310 C92311 C92312 C92313 C92314 C92315 C92316 C92317 C92318 C92319 C92320 C92321 C92322 0.097 0.106 0.093 0.114 0.100 0.102 0.099 0.107 0.097 0.108 0.101 0.103 0.104 0.107 0.105 0.121 0.101 0.106 0.102 0.109 0.093 0.097 0.090 0.105 0.104 0.094 0.114 0.108 0.112 0.111 0.117 0.081 0.094 0.076 0.109 0.085 0.086 0.081 0.087 0.086 0.087 0.087 0.086 0.086 0.090 0.091 0.093 0.085 0.090 0.087 0.089 0.080 0.081 0.078 0.091 0.106 0.081 0.096 0.095 0.097 0.094 0.093 0.076 0.037 0.067 0.089 0.076 0.078 0.074 0.080 0.077 0.081 0.076 0.080 0.078 0.078 0.080 0.086 0.076 0.080 0.077 0.080 0.076 0.072 0.072 0.078 SPILLED 0.072 0.086 SPILLED 0.085 0.080 0.078 0.071 0.074 0.063 0.076 0.068 0.071 0.068 0.076 NOT TAKEN 0.072 0.068 0.071 0.072 0.073 0.069 0.077 0.070 0.070 0.072 0.073 0.069 0.065 0.065 0.070 0.079 0.068 0.082 0.074 0.077 0.075 0.078 0.067 SPILLED 0.060 0.068 0.063 0.064 0.065 0.069 NOT TAKEN 0.069 0.064 0.068 0.066 0.069 0.070 0.074 0.064 0.066 0.066 0.069 0.066 0.064 0.065 0.072 SPILLED 0.064 SPILLED 0.070 0.072 0.069 0.068 0.064 0.067 0.059 0.062 0.058 0.060 0.060 0.068 NOT TAKEN 0.063 0.059 0.063 0.063 0.068 0.061 0.069 0.062 0.065 0.062 0.065 0.064 0.062 0.058 0.068 0.064 0.059 0.065 0.062 0.067 0.062 0.062 0.062 0.064 0.059 0.059 0.056 0.058 0.057 0.067 NOT TAKEN 0.055 0.056 0.059 0.060 0.063 0.060 0.067 0.062 0.063 0.060 0.061 0.066 0.063 0.059 0.068 0.057 0.057 0.064 0.063 0.065 0.064 0.059 0.058 0.060 0.061 0.059 0.053 0.059 0.053 SPILLED NOT TAKEN 0.056 0.056 0.055 0.057 0.055 0.060 0.064 0.059 0.068 0.062 0.061 0.060 0.057 0.056 0.062 0.055 0.055 0.068 0.055 0.060 0.058 0.057 0.057 0.058 0.062 0.055 0.053 0.059 0.052 SPILLED NOT TAKEN 0.055 0.053 0.054 0.058 0.057 0.057 0.061 0.058 0.060 0.053 0.056 0.060 0.058 0.054 0.060 0.051 0.054 0.058 0.055 0.059 0.060 0.055 0.058 0.058 0.058 0.054 0.053 0.060 0.049 0.061 NOT TAKEN 0.054 0.050 0.052 0.055 0.053 0.057 0.057 0.057 0.057 0.053 0.057 0.059 0.056 0.053 0.059 0.049 0.052 0.056 0.054 0.058 0.057 0.053 4086 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92323 C92324 C92325 C92326 C92327 C92328 C92329 C92330 0.116 0.097 0.102 0.116 0.106 0.104 0.108 0.096 0.093 0.083 0.087 0.099 0.092 0.089 0.096 0.082 0.085 0.076 0.080 0.083 0.081 0.081 0.086 0.076 0.076 0.071 0.072 0.075 0.076 0.077 NOT TAKEN 0.072 0.067 0.070 0.065 0.067 0.072 SPILLED NOT TAKEN 0.064 0.063 0.061 0.063 0.062 0.065 SPILLED NOT TAKEN 0.062 0.059 0.064 0.063 0.059 0.061 SPILLED NOT TAKEN 0.059 0.059 0.059 0.056 0.057 0.056 SPILLED NOT TAKEN 0.055 0.056 0.056 0.055 0.057 0.053 0.064 NOT TAKEN 0.054 0.054 0.056 0.054 0.053 0.053 0.059 NOT TAKEN 0.054 GROUP: FEMALE 9 - 1 PPM C92401 C92402 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 C92412 C92413 C92414 C92415 C92416 C92417 C92418 C92419 C92420 0.107 0.108 0.097 0.113 0.126 0.091 0.109 0.101 0.131 0.095 0.112 0.103 SPILLED 0.090 0.116 0.138 SPILLED 0.102 SPILLED SPILLED 0.089 0.099 0.091 0.099 0.092 0.109 0.105 0.090 SPILLED 0.084 SPILLED SPILLED SPILLED 0.076 0.102 SPILLED SPILLED 0.089 SPILLED SPILLED 0.087 0.091 0.090 0.091 0.083 0.086 0.092 0.082 0.087 0.076 0.129 0.115 0.088 0.073 0.098 0.101 0.092 0.084 0.089 SPILLED 0.082 NOT TAKEN 0.079 0.094 0.080 0.076 0.093 0.081 0.081 0.071 SPILLED SPILLED 0.083 0.081 0.094 0.087 0.099 0.082 0.095 SPILLED 0.081 NOT TAKEN 0.080 0.083 0.076 SPILLED 0.081 0.076 0.084 0.071 SPILLED SPILLED 0.078 0.073 0.087 0.083 0.086 0.073 SPILLED SPILLED 0.074 NOT TAKEN 0.076 0.076 0.092 0.090 0.077 0.069 0.090 0.064 SPILLED 0.080 0.073 0.067 0.079 0.073 0.070 0.066 SPILLED SPILLED 0.072 NOT TAKEN 0.070 0.076 0.082 0.077 0.071 0.068 0.079 0.066 0.072 0.090 0.074 0.069 0.073 0.070 0.071 0.060 SPILLED 0.081 0.067 NOT TAKEN 0.062 0.070 SPILLED 0.073 0.067 0.069 SPILLED 0.067 SPILLED SPILLED 0.069 0.069 0.077 0.067 0.073 0.060 SPILLED SPILLED 0.067 NOT TAKEN 0.061 0.072 0.063 0.072 0.062 0.064 0.069 0.060 SPILLED SPILLED 0.074 0.067 0.072 0.066 0.067 0.059 0.081 0.077 0.063 NOT TAKEN 0.067 0.069 0.077 0.070 0.064 0.059 0.072 0.063 SPILLED 0.061 SPILLED 0.069 0.067 0.068 0.065 0.056 SPILLED SPILLED 4087 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92421 C92422 C92423 C92424 C92425 C92426 C92427 C92428 C92429 C92430 C92431 C92432 C92433 C92434 C92435 C92436 C92437 C92438 C92439 C92440 C92441 C92442 C92443 C92444 C92445 C92446 C92447 C92448 C92449 C92450 C92451 0.099 0.098 SPILLED SPILLED 0.123 0.113 0.093 0.094 SPILLED 0.107 0.101 0.102 SPILLED 0.110 0.108 0.106 0.096 0.111 0.102 SPILLED 0.095 0.110 0.109 SPILLED 0.109 0.113 0.099 0.119 SPILLED 0.102 0.098 0.088 0.096 SPILLED 0.121 0.100 0.107 0.102 0.087 SPILLED 0.091 0.089 0.088 SPILLED 0.086 0.094 0.086 0.079 0.092 0.089 0.124 0.097 0.099 0.092 0.104 0.101 0.098 0.093 0.102 0.101 0.087 0.091 0.080 0.080 0.144 0.118 0.095 0.098 0.077 0.088 0.091 0.104 0.082 0.084 0.126 0.089 0.081 0.087 0.078 0.086 0.079 0.111 0.082 0.097 0.088 0.103 0.089 0.103 0.107 0.087 0.094 0.086 0.080 0.075 0.076 SPILLED SPILLED 0.089 0.086 0.082 0.078 0.089 0.082 0.078 0.081 0.131 0.075 NOT TAKEN 0.081 0.073 0.082 0.079 0.098 0.079 0.091 0.083 SPILLED 0.085 0.091 0.086 0.090 0.091 0.078 0.085 0.073 0.077 SPILLED SPILLED 0.085 0.076 SPILLED 0.073 0.083 0.086 0.075 0.077 SPILLED 0.077 NOT TAKEN 0.080 0.076 0.080 0.074 0.080 0.078 0.083 0.081 0.088 0.080 0.082 0.080 0.080 0.081 0.082 0.079 0.082 0.075 0.080 SPILLED 0.076 0.086 SPILLED 0.072 0.086 0.071 0.074 0.070 SPILLED 0.075 NOT TAKEN 0.073 0.073 0.075 0.068 0.076 0.071 0.080 0.081 SPILLED 0.074 SPILLED 0.075 0.072 SPILLED 0.070 0.074 0.068 0.069 0.087 SPILLED 0.072 0.074 0.088 0.071 0.073 0.075 0.071 0.068 SPILLED 0.071 NOT TAKEN 0.073 0.067 0.069 0.068 0.072 0.069 0.083 0.075 0.088 0.070 0.080 NOT TAKEN 0.069 0.099 0.068 0.064 SPILLED 0.066 SPILLED 0.089 0.077 0.074 0.123 SPILLED SPILLED 0.068 0.071 0.073 SPILLED 0.073 NOT TAKEN 0.065 0.067 0.068 0.073 0.071 0.076 0.073 0.073 SPILLED 0.064 0.068 EXCLUDE 0.068 SPILLED 0.066 0.063 SPILLED 0.066 SPILLED 0.082 0.069 SPILLED 0.070 0.064 0.068 0.065 0.072 0.064 0.068 0.067 NOT TAKEN 0.066 0.063 0.063 0.061 0.072 0.060 0.068 0.061 SPILLED 0.061 0.063 0.067 0.066 0.078 0.061 0.067 SPILLED 0.066 SPILLED SPILLED 0.069 0.065 0.069 0.063 0.069 0.064 0.066 0.065 SPILLED 0.070 NOT TAKEN 0.063 0.067 0.060 0.060 0.065 0.062 0.068 0.069 SPILLED 0.065 0.073 SPILLED 0.065 SPILLED 0.065 0.069 4088 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92452 C92453 C92454 C92455 C92456 C92457 C92458 C92459 C92460 C92461 C92462 C92463 C92464 C92465 C92466 C92467 C92468 C92469 C92470 0.113 0.104 0.119 0.107 SPILLED 0.110 0.109 0.109 0.126 0.122 0.106 0.106 0.114 0.114 0.111 0.106 0.116 SPILLED 0.110 SPILLED 0.087 0.104 0.091 SPILLED 0.090 0.100 0.098 SPILLED 0.112 SPILLED 0.096 0.097 0.103 0.097 0.088 0.092 0.112 0.104 SPILLED 0.091 0.093 0.089 SPILLED 0.088 0.095 0.089 SPILLED 0.087 0.095 0.088 0.088 0.099 0.091 0.098 0.088 0.108 0.091 SPILLED 0.081 0.090 0.086 0.141 0.080 0.089 0.086 0.092 0.086 0.087 NOT TAKEN 0.089 0.092 0.084 NOT TAKEN 0.076 0.091 NOT TAKEN SPILLED 0.080 0.081 0.081 SPILLED 0.083 0.083 0.081 0.097 SPILLED 0.080 NOT TAKEN 0.081 0.085 0.079 NOT TAKEN 0.076 0.086 NOT TAKEN SPILLED SPILLED 0.086 0.072 SPILLED 0.072 0.084 0.077 0.092 0.082 SPILLED NOT TAKEN 0.081 0.087 0.077 NOT TAKEN 0.074 0.096 NOT TAKEN SPILLED SPILLED SPILLED SPILLED SPILLED 0.064 0.070 0.075 0.082 0.071 0.087 NOT TAKEN 0.082 0.076 0.072 NOT TAKEN 0.077 0.095 NOT TAKEN SPILLED 0.073 0.071 0.071 SPILLED SPILLED 0.074 0.075 0.092 SPILLED 0.078 NOT TAKEN 0.070 0.076 0.078 NOT TAKEN 0.074 0.075 NOT TAKEN 0.084 0.071 0.069 0.065 0.080 0.072 0.071 0.071 0.122 SPILLED 0.080 NOT TAKEN 0.071 0.070 0.075 NOT TAKEN 0.071 0.073 NOT TAKEN 0.079 0.074 0.071 0.071 SPILLED 0.064 0.076 0.070 SPILLED 0.076 0.075 NOT TAKEN 0.073 SPILLED 0.069 NOT TAKEN 0.129 0.072 NOT TAKEN 4089 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92261 C92262 C92263 C92264 C92265 C92266 C92267 C92268 C92269 C92270 C92271 C92272 C92273 C92274 C92275 C92276 C92277 C92278 C92279 C92280 C92281 C92282 C92283 C92284 C92285 C92286 C92287 C92288 C92289 C92290 C92291 0.050 0.052 0.049 NOT TAKEN SPILLED 0.053 0.047 0.051 SPILLED 0.057 0.049 0.052 0.054 0.053 0.053 0.052 0.052 0.049 0.054 NOT TAKEN 0.050 0.053 SPILLED NOT TAKEN 0.054 0.049 0.048 0.051 0.055 0.056 0.047 0.052 0.053 0.049 NOT TAKEN 0.060 0.053 0.047 0.051 SPILLED 0.067 0.052 0.052 0.053 0.052 0.053 0.052 0.051 0.051 0.053 NOT TAKEN 0.048 0.051 0.056 NOT TAKEN 0.050 0.050 0.049 0.052 0.053 0.054 0.048 0.050 0.051 0.049 NOT TAKEN 0.059 0.052 0.047 0.052 SPILLED 0.056 0.052 0.055 0.054 0.051 0.051 0.052 0.051 0.050 0.055 NOT TAKEN 0.051 0.051 0.057 NOT TAKEN 0.052 0.049 0.050 0.050 0.054 0.056 0.048 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.052 0.049 0.046 0.049 0.052 0.048 0.047 0.051 NOT TAKEN 0.048 0.049 0.052 NOT TAKEN 0.050 0.045 0.047 0.048 0.051 0.053 0.049 0.050 0.049 0.049 NOT TAKEN 0.057 0.050 0.045 SPILLED SPILLED 0.060 0.047 0.050 0.051 0.046 0.049 0.050 0.046 0.048 0.051 NOT TAKEN 0.050 0.049 0.052 NOT TAKEN 0.049 0.044 0.048 0.047 0.051 0.053 0.048 0.050 0.050 0.048 NOT TAKEN 0.054 0.049 0.044 0.049 0.047 0.054 0.046 0.050 0.052 0.046 0.047 0.049 0.045 0.046 0.049 NOT TAKEN 0.047 0.048 0.050 NOT TAKEN 0.048 0.047 0.047 0.048 0.050 0.050 0.045 0.049 0.045 0.044 NOT TAKEN 0.048 0.039 0.042 0.044 0.050 0.047 0.045 0.047 SPILLED 0.044 0.046 0.046 0.041 0.041 0.043 NOT TAKEN 0.044 0.047 0.044 NOT TAKEN 0.042 0.041 0.044 0.044 0.046 0.042 0.043 0.047 0.043 0.042 NOT TAKEN 0.044 0.044 0.042 0.042 0.034 0.045 0.043 0.049 0.049 0.043 0.044 0.045 0.038 0.041 0.045 NOT TAKEN 0.043 0.049 0.044 NOT TAKEN 0.040 0.039 0.043 0.042 0.044 0.043 0.045 0.048 0.046 0.041 NOT TAKEN 0.050 0.046 0.042 0.043 SPILLED 0.049 0.043 SPILLED SPILLED 0.042 0.044 0.047 0.041 0.040 0.043 NOT TAKEN 0.044 0.048 0.044 NOT TAKEN 0.040 0.039 0.041 0.043 0.044 0.041 0.045 0.048 0.042 0.040 NOT TAKEN 0.048 0.043 0.039 0.041 0.045 0.045 0.041 0.045 0.046 0.042 0.043 0.044 0.040 0.040 0.041 NOT TAKEN 0.043 0.049 0.040 NOT TAKEN 0.039 0.036 0.043 0.039 0.044 0.041 0.044 4090 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92292 C92293 C92294 C92295 C92296 C92297 C92298 C92299 C92300 C92301 C92302 C92303 C92304 C92305 C92306 C92307 C92308 C92309 C92310 C92311 C92312 C92313 C92314 C92315 C92316 C92317 C92318 C92319 C92320 C92321 C92322 0.058 0.056 0.055 0.053 0.051 0.057 0.048 0.060 NOT TAKEN 0.052 0.050 0.050 0.050 0.051 0.056 0.056 0.054 0.055 0.050 0.052 0.055 0.054 0.052 0.058 0.049 0.051 0.056 0.054 0.058 0.056 0.056 0.057 0.061 0.052 0.052 0.052 0.058 0.049 0.062 NOT TAKEN 0.054 0.050 0.050 0.052 0.053 0.056 0.057 0.056 0.056 0.051 0.052 0.056 0.054 0.052 0.057 0.051 0.048 0.057 0.056 0.056 0.057 0.055 0.056 0.057 0.053 0.049 0.051 0.056 0.051 0.060 NOT TAKEN 0.052 0.049 0.051 0.051 0.054 0.056 0.055 0.058 0.053 0.052 0.052 0.056 0.055 0.052 0.054 0.047 0.048 0.056 0.054 0.052 0.055 0.053 0.054 0.055 0.049 0.047 0.049 0.058 0.047 0.054 NOT TAKEN 0.052 0.045 0.049 0.050 0.053 0.054 0.054 0.051 0.050 0.049 0.049 0.054 0.053 0.049 0.054 0.050 0.048 0.053 0.053 0.054 0.053 0.051 0.052 0.051 0.052 0.051 0.048 0.055 0.047 0.056 NOT TAKEN 0.053 0.044 0.048 0.048 0.052 0.054 0.052 0.050 0.050 0.051 0.050 0.054 0.054 0.051 0.055 0.048 0.048 0.051 0.051 0.052 0.055 0.050 0.052 0.052 0.050 0.048 0.047 0.053 0.045 0.055 NOT TAKEN 0.050 0.045 0.049 0.047 0.051 0.053 0.052 0.049 0.049 0.050 0.049 0.053 0.051 0.051 0.054 0.047 0.046 0.055 0.045 0.052 0.054 0.049 0.049 0.047 0.042 0.041 0.046 0.053 0.039 SPILLED NOT TAKEN 0.047 0.041 0.045 0.046 0.043 0.046 0.046 SPILLED 0.046 0.043 0.046 0.052 0.047 0.046 0.048 0.041 0.041 0.049 0.052 0.048 0.049 0.048 0.049 0.047 0.042 0.044 0.044 0.054 0.043 0.050 NOT TAKEN 0.049 0.041 0.045 0.044 0.042 0.045 0.048 0.051 0.046 0.039 0.045 0.051 0.044 0.045 0.045 0.041 0.040 0.047 0.049 0.046 0.050 0.047 0.046 0.047 0.042 0.043 0.044 0.054 0.040 SPILLED NOT TAKEN 0.045 0.037 0.046 0.046 0.043 0.041 0.048 0.049 0.047 0.039 0.040 0.050 0.045 0.045 0.042 0.042 0.040 0.045 0.053 0.045 0.049 0.047 0.045 0.045 0.042 0.042 0.044 0.054 0.040 0.049 NOT TAKEN 0.045 0.041 0.044 0.046 0.041 0.042 0.046 0.053 0.046 0.037 0.042 0.046 0.043 0.045 0.042 0.040 0.038 0.043 0.048 0.043 0.046 0.043 4091 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92323 C92324 C92325 C92326 C92327 C92328 C92329 C92330 0.053 0.053 0.052 0.052 0.052 SPILLED NOT TAKEN 0.051 0.053 0.056 0.052 0.057 0.051 0.055 NOT TAKEN 0.050 0.054 0.052 0.051 0.052 0.049 0.053 NOT TAKEN 0.050 0.051 0.053 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.050 0.052 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.050 0.053 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.045 0.048 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.044 0.047 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.046 0.048 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.043 0.045 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN GROUP: FEMALE 9 - 1 PPM C92401 C92402 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 C92412 C92413 C92414 C92415 C92416 C92417 C92418 C92419 C92420 0.062 NOT TAKEN 0.057 0.063 SPILLED 0.063 0.059 0.060 0.061 0.057 SPILLED SPILLED 0.060 0.057 0.060 0.054 0.062 0.056 SPILLED 0.065 0.061 NOT TAKEN 0.070 0.065 0.063 SPILLED 0.059 0.061 0.063 0.061 SPILLED SPILLED 0.059 0.062 0.064 0.062 0.064 0.059 0.074 SPILLED 0.061 NOT TAKEN 0.063 0.064 0.069 0.062 0.057 0.058 0.062 0.060 0.096 0.073 0.065 0.061 0.055 0.063 0.060 0.060 SPILLED 0.078 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.075 0.059 0.063 SPILLED 0.056 0.053 0.093 0.079 0.058 NOT TAKEN 0.056 0.062 0.071 0.067 0.060 0.057 0.065 0.058 0.069 0.062 0.068 0.056 0.059 SPILLED 0.056 0.054 SPILLED 0.083 0.060 NOT TAKEN 0.056 0.065 0.061 0.068 0.058 0.059 0.059 0.056 SPILLED SPILLED 0.069 0.056 0.056 0.065 0.058 0.052 SPILLED SPILLED 0.055 NOT TAKEN 0.054 0.060 0.056 0.067 0.060 0.055 0.057 0.053 SPILLED SPILLED 0.060 0.057 0.059 0.068 0.051 0.061 SPILLED SPILLED 0.054 NOT TAKEN 0.052 0.060 0.058 0.071 0.055 0.054 0.062 0.055 0.081 0.056 0.059 0.054 0.052 0.060 0.056 0.052 SPILLED SPILLED 0.057 NOT TAKEN 0.054 0.055 0.067 0.072 0.061 0.059 0.063 0.053 0.076 0.061 0.057 0.057 0.057 0.063 0.059 0.050 SPILLED 0.092 0.054 NOT TAKEN 0.048 0.058 0.061 0.067 0.054 0.053 0.057 0.057 0.068 0.071 0.048 0.052 0.056 0.063 0.052 0.050 0.069 0.081 4092 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92421 C92422 C92423 C92424 C92425 C92426 C92427 C92428 C92429 C92430 C92431 C92432 C92433 C92434 C92435 C92436 C92437 C92438 C92439 C92440 C92441 C92442 C92443 C92444 C92445 C92446 C92447 C92448 C92449 C92450 C92451 SPILLED 0.058 SPILLED SPILLED 0.059 SPILLED SPILLED 0.060 0.069 0.060 0.055 0.057 0.055 0.063 NOT TAKEN 0.065 0.068 0.058 0.057 0.059 0.061 0.056 0.062 SPILLED 0.054 0.061 0.065 0.060 SPILLED 0.059 0.065 0.066 0.059 0.073 0.082 0.062 0.068 0.080 0.063 0.069 0.068 0.056 0.064 0.059 0.063 NOT TAKEN 0.062 0.067 0.060 0.061 0.064 0.062 0.056 0.058 SPILLED 0.056 SPILLED SPILLED 0.065 SPILLED 0.065 0.070 0.063 0.060 0.082 0.078 0.065 0.071 SPILLED 0.060 0.063 0.068 0.064 0.060 0.056 0.067 NOT TAKEN 0.065 0.071 0.062 0.058 0.071 0.060 0.062 0.065 SPILLED 0.061 0.063 0.065 0.068 0.067 0.071 0.066 0.059 0.061 SPILLED SPILLED 0.062 SPILLED SPILLED 0.053 SPILLED 0.060 0.062 0.064 SPILLED 0.070 NOT TAKEN 0.059 0.063 0.060 0.059 0.061 SPILLED 0.062 0.057 SPILLED 0.056 0.051 0.061 0.068 0.082 0.060 0.068 0.059 0.058 SPILLED 0.087 0.058 SPILLED SPILLED 0.056 0.061 0.058 0.054 0.057 0.055 0.065 NOT TAKEN 0.060 0.058 0.057 0.060 0.068 0.056 0.056 0.064 SPILLED 0.054 0.061 0.067 0.062 0.079 0.059 0.064 0.055 0.054 SPILLED SPILLED 0.058 SPILLED SPILLED 0.059 0.064 0.059 0.050 0.061 0.060 0.070 NOT TAKEN 0.063 0.059 0.060 0.063 0.063 0.058 0.055 0.056 SPILLED 0.052 0.061 SPILLED 0.063 0.069 0.063 0.072 0.055 0.055 0.071 0.105 0.057 0.117 SPILLED 0.055 0.061 0.058 0.053 0.062 0.076 0.066 NOT TAKEN 0.056 0.062 0.059 0.063 0.056 0.066 0.053 0.059 SPILLED 0.049 0.056 0.066 0.057 0.060 0.057 0.071 0.059 0.057 SPILLED SPILLED 0.059 SPILLED 0.060 0.055 0.061 0.056 0.058 0.062 0.055 0.060 NOT TAKEN 0.057 0.064 0.061 0.057 0.061 0.062 0.058 0.054 0.085 0.049 0.054 0.063 0.054 0.060 0.056 0.062 0.061 0.060 0.073 0.086 0.054 0.075 0.059 0.053 0.064 0.057 0.061 0.063 0.058 0.067 NOT TAKEN 0.068 0.076 0.062 0.055 0.064 0.060 0.053 0.055 SPILLED 0.046 0.060 0.067 0.055 0.064 0.057 0.063 0.061 0.056 0.067 0.085 0.059 0.068 0.067 0.044 0.075 0.055 0.058 0.059 0.057 0.063 NOT TAKEN 0.055 0.068 0.061 0.051 0.057 0.059 0.050 0.054 0.087 0.044 0.061 0.062 0.055 0.063 0.053 0.060 4093 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92452 C92453 C92454 C92455 C92456 C92457 C92458 C92459 C92460 C92461 C92462 C92463 C92464 C92465 C92466 C92467 C92468 C92469 C92470 0.075 0.076 0.068 0.075 0.073 0.061 0.069 0.061 SPILLED 0.074 0.090 NOT TAKEN 0.070 0.067 SPILLED NOT TAKEN SPILLED 0.069 NOT TAKEN 0.078 0.070 0.075 0.061 SPILLED 0.065 0.070 0.069 SPILLED 0.074 0.077 NOT TAKEN 0.075 0.072 0.072 NOT TAKEN 0.068 0.066 NOT TAKEN 0.081 0.068 0.069 0.064 SPILLED 0.067 0.069 0.072 0.105 0.079 0.088 NOT TAKEN 0.073 0.065 0.077 NOT TAKEN 0.063 0.070 NOT TAKEN 0.081 0.069 0.071 0.068 0.069 0.063 0.058 0.070 SPILLED SPILLED 0.075 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.080 0.066 0.066 0.062 0.068 0.061 0.064 0.075 0.126 0.077 0.078 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.079 0.063 0.064 0.064 0.074 0.062 0.069 0.071 SPILLED SPILLED 0.077 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.055 0.053 0.056 0.081 0.063 0.063 0.069 SPILLED 0.069 0.076 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.067 0.051 0.053 0.052 0.057 0.057 0.059 0.067 0.100 0.070 0.070 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.069 0.058 0.061 0.058 0.061 0.059 0.059 0.060 SPILLED 0.074 0.073 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.054 0.057 0.056 0.058 0.066 0.056 0.037 0.081 0.069 0.064 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4094 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92261 C92262 C92263 C92264 C92265 C92266 C92267 C92268 C92269 C92270 C92271 C92272 C92273 C92274 C92275 C92276 C92277 C92278 C92279 C92280 C92281 C92282 C92283 C92284 C92285 C92286 C92287 C92288 C92289 C92290 C92291 0.047 0.041 0.038 NOT TAKEN 0.045 0.038 0.039 0.037 0.042 0.043 0.040 SPILLED 0.039 0.040 0.042 0.042 0.038 0.038 0.040 NOT TAKEN 0.041 0.046 0.040 NOT TAKEN 0.037 0.035 0.039 0.038 0.041 0.038 0.043 0.044 0.040 0.036 NOT TAKEN 0.045 0.038 0.038 0.037 0.040 0.040 0.038 0.040 0.038 0.039 0.039 0.042 0.036 0.037 0.039 NOT TAKEN 0.039 0.042 0.038 NOT TAKEN 0.035 0.034 0.038 0.037 0.039 0.037 0.041 0.042 0.037 0.036 NOT TAKEN 0.049 0.037 0.037 0.035 0.038 0.040 0.035 0.039 NOT TAKEN 0.033 0.039 0.040 0.035 0.034 0.035 NOT TAKEN 0.037 0.043 0.037 NOT TAKEN 0.034 0.032 0.036 0.035 0.037 0.034 0.038 0.040 0.038 0.035 NOT TAKEN 0.043 0.036 0.036 0.035 0.036 0.039 0.035 0.037 NOT TAKEN 0.036 0.039 0.038 0.035 0.036 0.034 NOT TAKEN 0.038 0.041 0.036 NOT TAKEN 0.034 0.032 0.037 0.036 0.036 0.033 0.040 0.037 0.036 0.033 NOT TAKEN 0.039 0.035 0.034 0.032 0.036 0.037 0.034 0.035 NOT TAKEN 0.035 0.036 0.041 0.034 0.033 0.035 NOT TAKEN 0.036 0.037 0.036 NOT TAKEN 0.031 0.031 0.033 0.034 0.036 0.031 0.038 0.038 0.036 0.032 NOT TAKEN 0.038 0.035 NOT TAKEN 0.031 0.034 0.036 0.033 0.035 NOT TAKEN NOT TAKEN 0.036 0.040 0.029 0.034 0.034 NOT TAKEN 0.033 0.038 0.035 NOT TAKEN 0.031 NOT TAKEN NOT TAKEN NOT TAKEN 0.038 0.017 0.035 0.038 0.035 0.035 NOT TAKEN 0.037 0.035 NOT TAKEN 0.031 0.035 0.036 0.034 0.038 NOT TAKEN NOT TAKEN 0.037 0.040 NOT TAKEN 0.034 0.036 NOT TAKEN 0.035 0.038 0.033 NOT TAKEN 0.030 NOT TAKEN NOT TAKEN NOT TAKEN 0.042 0.017 0.039 0.038 0.036 0.034 NOT TAKEN 0.038 0.035 NOT TAKEN 0.033 0.036 0.035 0.035 0.036 NOT TAKEN NOT TAKEN 0.037 0.042 NOT TAKEN 0.034 0.035 NOT TAKEN 0.037 0.040 0.034 NOT TAKEN 0.029 NOT TAKEN NOT TAKEN NOT TAKEN 0.038 NOT TAKEN 0.040 0.037 0.036 0.033 NOT TAKEN 0.038 0.035 NOT TAKEN 0.031 0.036 0.037 0.033 0.038 NOT TAKEN NOT TAKEN 0.038 0.042 NOT TAKEN 0.032 0.035 NOT TAKEN 0.037 0.033 0.035 NOT TAKEN 0.032 NOT TAKEN NOT TAKEN NOT TAKEN 0.037 NOT TAKEN 0.040 0.035 0.034 0.034 NOT TAKEN 0.037 0.035 NOT TAKEN 0.031 0.034 0.036 0.033 0.034 NOT TAKEN NOT TAKEN 0.037 0.013 NOT TAKEN 0.036 0.037 NOT TAKEN 0.037 0.035 0.034 NOT TAKEN 0.014 NOT TAKEN NOT TAKEN NOT TAKEN 0.035 NOT TAKEN 0.040 4095 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92292 C92293 C92294 C92295 C92296 C92297 C92298 C92299 C92300 C92301 C92302 C92303 C92304 C92305 C92306 C92307 C92308 C92309 C92310 C92311 C92312 C92313 C92314 C92315 C92316 C92317 C92318 C92319 C92320 C92321 C92322 0.043 0.043 0.040 0.039 0.041 SPILLED 0.036 0.047 NOT TAKEN 0.042 0.037 SPILLED 0.042 0.040 0.038 0.041 0.053 0.043 0.037 SPILLED 0.045 0.042 0.041 0.040 0.038 0.038 0.039 0.047 0.042 0.044 0.042 0.041 0.042 0.038 0.036 0.039 0.046 0.036 0.046 NOT TAKEN 0.038 0.035 0.039 0.041 0.038 0.038 0.042 0.053 0.041 0.036 0.039 0.042 0.041 0.040 0.039 0.036 0.037 0.038 0.043 0.040 0.042 0.039 0.037 NOT TAKEN 0.040 0.037 0.038 0.046 0.039 0.044 NOT TAKEN 0.037 0.036 0.039 0.038 0.036 0.036 0.040 0.044 0.040 0.035 0.035 0.041 0.038 0.038 0.037 0.035 0.035 0.037 0.041 0.040 0.040 0.038 0.035 NOT TAKEN 0.035 0.038 0.037 0.044 0.036 0.042 NOT TAKEN 0.036 0.033 0.037 0.038 0.035 0.037 0.039 0.048 0.040 0.034 0.035 0.041 0.038 0.037 0.035 0.034 0.034 0.035 0.043 0.037 0.037 0.036 0.035 NOT TAKEN 0.033 0.038 0.033 0.043 0.034 SPILLED NOT TAKEN 0.036 0.032 0.036 0.036 0.036 0.035 0.037 0.045 0.036 0.034 0.034 0.040 0.036 0.037 0.037 0.035 0.032 0.033 0.038 0.037 0.040 0.034 0.026 NOT TAKEN 0.034 0.036 0.032 0.039 0.033 SPILLED NOT TAKEN 0.037 0.030 NOT TAKEN 0.035 0.037 0.034 0.041 0.056 0.035 0.030 0.034 0.043 0.035 0.035 NOT TAKEN 0.033 NOT TAKEN 0.034 0.038 NOT TAKEN 0.035 0.034 0.022 NOT TAKEN 0.035 0.037 0.032 0.039 0.034 SPILLED NOT TAKEN 0.037 0.033 NOT TAKEN 0.035 0.038 0.034 0.039 SPILLED 0.035 0.034 0.036 0.042 0.035 0.035 NOT TAKEN 0.033 NOT TAKEN 0.033 0.039 NOT TAKEN 0.036 0.035 0.022 NOT TAKEN 0.032 0.032 0.034 0.042 0.015 0.042 NOT TAKEN 0.037 0.022 NOT TAKEN 0.037 NOT TAKEN 0.034 0.042 SPILLED 0.038 0.036 0.035 0.042 0.038 0.037 NOT TAKEN 0.034 NOT TAKEN 0.034 0.039 NOT TAKEN 0.039 0.037 NOT TAKEN NOT TAKEN 0.025 0.033 0.032 0.043 NOT TAKEN 0.044 NOT TAKEN 0.035 0.032 NOT TAKEN 0.035 NOT TAKEN 0.034 0.043 0.054 0.038 0.036 0.034 NOT TAKEN 0.037 0.036 NOT TAKEN 0.033 NOT TAKEN 0.034 SPILLED NOT TAKEN 0.038 0.035 NOT TAKEN NOT TAKEN 0.029 0.036 0.035 0.043 NOT TAKEN 0.044 NOT TAKEN 0.036 0.032 NOT TAKEN 0.037 NOT TAKEN 0.039 0.043 SPILLED 0.038 0.021 0.035 NOT TAKEN 0.039 0.035 NOT TAKEN 0.035 NOT TAKEN 0.034 0.040 NOT TAKEN 0.040 0.035 4096 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92323 C92324 C92325 C92326 C92327 C92328 C92329 C92330 0.039 0.045 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.038 0.041 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.036 0.041 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.036 0.046 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.033 0.042 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.033 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.033 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.035 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.036 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.034 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN GROUP: FEMALE 9 - 1 PPM C92401 C92402 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 C92412 C92413 C92414 C92415 C92416 C92417 C92418 C92419 C92420 0.047 NOT TAKEN 0.049 0.054 0.058 0.069 0.049 0.057 0.062 0.055 0.064 0.050 0.048 0.049 0.053 0.060 0.053 0.045 0.056 0.067 0.045 NOT TAKEN 0.040 0.053 0.050 0.064 0.048 0.051 0.054 0.051 0.060 0.053 0.040 0.046 0.044 0.053 0.042 0.044 0.057 0.066 0.047 NOT TAKEN 0.041 0.044 0.053 0.054 0.050 0.055 0.055 0.048 0.058 0.057 0.044 0.043 0.048 0.054 0.050 0.045 0.053 0.058 0.043 NOT TAKEN 0.028 0.045 0.051 0.058 0.039 0.050 0.050 0.045 0.076 0.050 0.045 0.044 0.047 0.048 0.046 0.042 0.049 0.060 0.045 NOT TAKEN NOT TAKEN 0.043 0.054 0.059 0.048 0.053 0.053 0.049 0.059 SPILLED 0.049 0.040 0.042 0.050 0.046 0.041 0.049 SPILLED 0.038 NOT TAKEN NOT TAKEN 0.041 0.050 NOT TAKEN NOT TAKEN 0.046 0.048 0.048 NOT TAKEN 0.050 0.034 0.044 0.044 0.041 0.040 NOT TAKEN 0.050 0.054 0.043 NOT TAKEN NOT TAKEN 0.044 0.046 NOT TAKEN NOT TAKEN 0.044 0.052 0.047 NOT TAKEN SPILLED 0.046 0.043 0.046 0.046 0.042 NOT TAKEN 0.053 0.050 0.039 NOT TAKEN NOT TAKEN 0.041 0.049 NOT TAKEN NOT TAKEN 0.044 0.050 0.048 NOT TAKEN 0.049 0.053 NOT TAKEN 0.043 0.045 0.040 NOT TAKEN 0.079 0.051 0.042 NOT TAKEN NOT TAKEN 0.046 0.047 NOT TAKEN NOT TAKEN 0.049 0.047 0.048 NOT TAKEN 0.052 0.040 NOT TAKEN 0.042 0.053 0.045 NOT TAKEN 0.048 0.043 0.040 NOT TAKEN NOT TAKEN 0.042 0.053 NOT TAKEN NOT TAKEN 0.046 0.054 0.048 NOT TAKEN 0.048 0.049 NOT TAKEN 0.037 0.048 0.045 NOT TAKEN 0.057 0.042 4097 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92421 C92422 C92423 C92424 C92425 C92426 C92427 C92428 C92429 C92430 C92431 C92432 C92433 C92434 C92435 C92436 C92437 C92438 C92439 C92440 C92441 C92442 C92443 C92444 C92445 C92446 C92447 C92448 C92449 C92450 C92451 0.061 0.051 SPILLED SPILLED 0.057 0.061 SPILLED 0.055 0.057 0.055 0.059 0.060 0.057 0.061 NOT TAKEN 0.057 0.064 0.052 SPILLED 0.059 SPILLED 0.047 0.049 0.069 0.042 0.049 0.055 0.045 0.064 0.049 SPILLED 0.055 0.050 0.068 0.076 0.048 0.058 SPILLED 0.047 0.057 0.051 0.046 0.055 0.053 0.057 NOT TAKEN 0.053 0.057 0.055 0.047 0.053 0.057 0.045 0.042 SPILLED 0.040 0.054 0.054 0.049 0.064 0.046 0.051 0.051 0.046 0.055 SPILLED 0.047 0.061 0.064 0.046 0.051 0.054 0.053 0.055 0.049 0.054 NOT TAKEN 0.057 0.054 0.054 0.050 0.051 0.058 0.044 0.048 SPILLED 0.040 0.044 0.056 0.049 0.062 0.047 0.045 0.052 0.048 0.059 0.083 0.046 0.058 0.057 0.048 0.047 0.050 0.044 0.052 0.050 0.053 NOT TAKEN 0.055 0.034 0.050 0.045 0.061 0.052 0.042 0.044 SPILLED 0.037 0.044 0.052 0.044 0.062 0.042 0.043 0.045 SPILLED SPILLED SPILLED 0.045 0.060 0.082 0.040 0.044 0.045 0.038 0.055 0.047 0.055 NOT TAKEN 0.053 NOT TAKEN SPILLED 0.041 0.045 0.053 0.042 0.038 0.059 0.040 0.044 0.051 0.037 0.070 0.045 SPILLED 0.049 NOT TAKEN 0.049 NOT TAKEN 0.044 0.052 0.049 0.042 0.030 0.047 0.047 0.051 NOT TAKEN 0.052 NOT TAKEN 0.048 NOT TAKEN NOT TAKEN 0.047 0.048 NOT TAKEN 0.037 0.044 SPILLED 0.036 0.047 SPILLED 0.042 NOT TAKEN 0.041 SPILLED 0.056 NOT TAKEN 0.048 NOT TAKEN 0.046 0.051 SPILLED 0.026 0.043 0.046 0.041 0.050 NOT TAKEN 0.054 NOT TAKEN 0.052 NOT TAKEN NOT TAKEN 0.041 0.076 NOT TAKEN 0.037 0.045 SPILLED 0.037 0.039 0.048 0.040 NOT TAKEN 0.041 0.056 0.048 NOT TAKEN 0.045 NOT TAKEN 0.040 0.050 NOT TAKEN NOT TAKEN NOT TAKEN 0.044 0.046 0.051 NOT TAKEN 0.056 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 0.045 0.044 NOT TAKEN 0.019 0.040 0.057 0.035 0.040 0.043 0.018 NOT TAKEN 0.042 0.057 0.053 NOT TAKEN 0.051 NOT TAKEN 0.044 0.051 NOT TAKEN NOT TAKEN NOT TAKEN 0.049 0.048 0.051 NOT TAKEN 0.051 NOT TAKEN 0.052 NOT TAKEN NOT TAKEN 0.048 0.048 NOT TAKEN NOT TAKEN 0.037 0.053 0.033 0.041 0.044 0.028 NOT TAKEN 0.045 0.058 0.050 NOT TAKEN 0.049 NOT TAKEN 0.042 0.050 NOT TAKEN NOT TAKEN NOT TAKEN 0.045 0.038 0.030 NOT TAKEN 0.051 NOT TAKEN 0.056 NOT TAKEN NOT TAKEN 0.050 0.054 NOT TAKEN NOT TAKEN 0.042 0.050 0.031 0.040 0.034 NOT TAKEN NOT TAKEN 0.042 0.057 4098 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92452 C92453 C92454 C92455 C92456 C92457 C92458 C92459 C92460 C92461 C92462 C92463 C92464 C92465 C92466 C92467 C92468 C92469 C92470 0.073 0.053 0.057 0.053 0.062 0.057 0.053 0.057 0.062 0.064 0.074 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.064 0.048 0.054 0.053 0.059 0.051 0.048 0.056 SPILLED 0.060 0.067 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.064 0.045 0.053 0.051 0.056 0.048 0.044 0.058 0.054 0.049 0.073 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.059 0.047 0.053 0.046 0.054 0.046 0.045 0.055 0.054 0.058 0.063 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.059 0.042 0.047 0.046 0.052 0.048 0.042 0.055 0.048 0.060 0.051 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.042 0.049 0.042 0.049 SPILLED 0.045 0.054 0.046 0.053 0.053 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.047 0.045 0.043 0.043 0.045 0.037 0.048 0.048 0.023 0.043 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.040 0.036 0.045 0.050 0.042 0.001 0.050 0.047 NOT TAKEN 0.052 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.070 0.048 0.046 0.039 0.046 0.052 NOT TAKEN 0.052 0.051 NOT TAKEN 0.052 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.046 0.041 0.044 0.047 0.047 NOT TAKEN 0.052 0.051 NOT TAKEN 0.048 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4099 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92261 C92262 C92263 C92264 C92265 C92266 C92267 C92268 C92269 C92270 C92271 C92272 C92273 C92274 C92275 C92276 C92277 C92278 C92279 C92280 C92281 C92282 C92283 C92284 C92285 C92286 C92287 C92288 C92289 C92290 C92291 0.035 0.033 0.033 NOT TAKEN 0.027 0.036 NOT TAKEN 0.029 NOT TAKEN 0.037 0.035 0.034 NOT TAKEN NOT TAKEN 0.037 NOT TAKEN NOT TAKEN 0.033 0.032 NOT TAKEN 0.036 0.036 0.038 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.037 NOT TAKEN 0.038 0.034 0.034 0.030 NOT TAKEN NOT TAKEN 0.032 NOT TAKEN 0.024 NOT TAKEN 0.034 SPILLED 0.032 NOT TAKEN NOT TAKEN 0.036 NOT TAKEN NOT TAKEN 0.031 0.024 NOT TAKEN 0.033 0.035 0.033 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.033 NOT TAKEN 0.038 0.032 0.035 0.031 NOT TAKEN NOT TAKEN 0.031 NOT TAKEN 0.022 NOT TAKEN 0.032 0.027 0.039 NOT TAKEN NOT TAKEN 0.037 NOT TAKEN NOT TAKEN 0.032 0.026 NOT TAKEN 0.030 0.034 0.032 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.031 NOT TAKEN 0.037 0.032 0.038 0.030 NOT TAKEN NOT TAKEN 0.029 NOT TAKEN 0.024 NOT TAKEN 0.033 0.027 NOT TAKEN NOT TAKEN NOT TAKEN 0.037 NOT TAKEN NOT TAKEN 0.036 SPILLED NOT TAKEN 0.032 0.034 0.030 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.031 NOT TAKEN 0.037 0.032 0.049 0.031 NOT TAKEN NOT TAKEN 0.028 NOT TAKEN 0.015 NOT TAKEN 0.031 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.035 NOT TAKEN NOT TAKEN 0.032 NOT TAKEN NOT TAKEN 0.030 0.031 0.030 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.030 NOT TAKEN 0.037 0.033 SPILLED 0.029 NOT TAKEN NOT TAKEN 0.028 NOT TAKEN 0.011 NOT TAKEN 0.031 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.034 NOT TAKEN NOT TAKEN 0.029 NOT TAKEN NOT TAKEN 0.030 0.033 0.027 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.029 NOT TAKEN NOT TAKEN 0.033 SPILLED 0.029 NOT TAKEN NOT TAKEN 0.031 NOT TAKEN NOT TAKEN NOT TAKEN 0.033 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.033 NOT TAKEN NOT TAKEN 0.034 NOT TAKEN NOT TAKEN 0.036 0.036 0.031 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.032 NOT TAKEN NOT TAKEN 0.037 NOT TAKEN 0.032 NOT TAKEN NOT TAKEN 0.030 NOT TAKEN NOT TAKEN NOT TAKEN 0.036 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.035 NOT TAKEN NOT TAKEN 0.037 0.035 0.031 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.034 NOT TAKEN NOT TAKEN 4100 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92292 C92293 C92294 C92295 C92296 C92297 C92298 C92299 C92300 C92301 C92302 C92303 C92304 C92305 C92306 C92307 C92308 C92309 C92310 C92311 C92312 C92313 C92314 C92315 C92316 C92317 C92318 C92319 C92320 C92321 C92322 NOT TAKEN NOT TAKEN 0.050 0.036 NOT TAKEN 0.041 NOT TAKEN 0.042 NOT TAKEN 0.035 0.034 NOT TAKEN 0.033 NOT TAKEN 0.034 0.042 0.052 0.038 NOT TAKEN 0.030 NOT TAKEN 0.037 0.037 NOT TAKEN 0.035 NOT TAKEN 0.035 0.034 NOT TAKEN 0.039 0.035 NOT TAKEN NOT TAKEN 0.039 0.031 NOT TAKEN 0.037 NOT TAKEN SPILLED NOT TAKEN 0.032 0.029 NOT TAKEN 0.034 NOT TAKEN 0.030 0.037 SPILLED 0.036 NOT TAKEN 0.032 NOT TAKEN 0.035 0.035 NOT TAKEN 0.030 NOT TAKEN 0.032 0.036 NOT TAKEN 0.040 0.029 NOT TAKEN NOT TAKEN 0.024 0.031 NOT TAKEN 0.036 NOT TAKEN 0.037 NOT TAKEN 0.029 0.027 NOT TAKEN 0.032 NOT TAKEN 0.032 0.034 SPILLED 0.034 NOT TAKEN 0.031 NOT TAKEN 0.035 0.034 NOT TAKEN SPILLED NOT TAKEN 0.032 0.033 NOT TAKEN 0.034 0.030 NOT TAKEN NOT TAKEN 0.018 0.030 NOT TAKEN 0.035 NOT TAKEN 0.037 NOT TAKEN NOT TAKEN 0.029 NOT TAKEN 0.029 NOT TAKEN 0.030 0.034 SPILLED 0.035 NOT TAKEN 0.029 NOT TAKEN 0.036 0.028 NOT TAKEN 0.032 NOT TAKEN 0.033 0.033 NOT TAKEN 0.036 0.031 NOT TAKEN NOT TAKEN 0.020 0.031 NOT TAKEN 0.032 NOT TAKEN 0.036 NOT TAKEN NOT TAKEN 0.017 NOT TAKEN 0.029 NOT TAKEN 0.029 0.034 0.047 0.033 NOT TAKEN 0.029 NOT TAKEN 0.034 SPILLED NOT TAKEN 0.027 NOT TAKEN 0.028 0.035 NOT TAKEN 0.033 0.032 NOT TAKEN NOT TAKEN 0.007 0.027 NOT TAKEN 0.032 NOT TAKEN 0.035 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.028 0.034 0.037 0.033 NOT TAKEN 0.030 NOT TAKEN 0.027 NOT TAKEN NOT TAKEN 0.020 NOT TAKEN 0.034 0.036 NOT TAKEN 0.033 0.029 NOT TAKEN NOT TAKEN NOT TAKEN 0.031 NOT TAKEN 0.036 NOT TAKEN 0.031 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.030 0.038 0.038 0.035 NOT TAKEN 0.034 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.031 NOT TAKEN 0.034 0.041 NOT TAKEN 0.037 0.033 NOT TAKEN NOT TAKEN NOT TAKEN 0.032 NOT TAKEN 0.034 NOT TAKEN 0.022 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.026 0.031 NOT TAKEN 0.032 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.032 0.035 NOT TAKEN 0.035 0.035 4101 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 9 - 1 PPM C92323 C92324 C92325 C92326 C92327 C92328 C92329 C92330 0.028 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.030 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.030 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.028 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.029 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.029 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.030 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.024 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN GROUP: FEMALE 9 - 1 PPM C92401 C92402 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 C92412 C92413 C92414 C92415 C92416 C92417 C92418 C92419 C92420 SPILLED NOT TAKEN NOT TAKEN 0.041 0.049 NOT TAKEN NOT TAKEN 0.047 0.048 0.048 NOT TAKEN 0.041 0.042 NOT TAKEN 0.040 0.049 0.045 NOT TAKEN 0.049 NOT TAKEN 0.028 NOT TAKEN NOT TAKEN 0.016 0.045 NOT TAKEN NOT TAKEN 0.043 SPILLED 0.042 NOT TAKEN 0.043 0.037 NOT TAKEN 0.043 0.045 0.039 NOT TAKEN 0.050 NOT TAKEN 0.019 NOT TAKEN NOT TAKEN NOT TAKEN 0.040 NOT TAKEN NOT TAKEN 0.042 0.046 0.043 NOT TAKEN 0.035 0.038 NOT TAKEN 0.043 0.042 0.013 NOT TAKEN 0.043 NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN 0.042 NOT TAKEN NOT TAKEN 0.045 0.044 0.043 NOT TAKEN 0.041 0.035 NOT TAKEN 0.030 0.041 0.009 NOT TAKEN 0.046 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.036 NOT TAKEN NOT TAKEN 0.041 0.035 0.042 NOT TAKEN 0.040 0.026 NOT TAKEN NOT TAKEN 0.039 0.042 NOT TAKEN 0.043 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.006 NOT TAKEN NOT TAKEN 0.048 0.006 0.040 NOT TAKEN 0.041 0.030 NOT TAKEN NOT TAKEN 0.031 NOT TAKEN NOT TAKEN 0.040 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN 0.040 NOT TAKEN 0.040 0.032 NOT TAKEN NOT TAKEN 0.044 NOT TAKEN NOT TAKEN 0.049 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.037 NOT TAKEN 0.042 NOT TAKEN NOT TAKEN NOT TAKEN 0.049 NOT TAKEN NOT TAKEN 0.045 NOT TAKEN 4102 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92421 C92422 C92423 C92424 C92425 C92426 C92427 C92428 C92429 C92430 C92431 C92432 C92433 C92434 C92435 C92436 C92437 C92438 C92439 C92440 C92441 C92442 C92443 C92444 C92445 C92446 C92447 C92448 C92449 C92450 C92451 0.046 NOT TAKEN 0.046 NOT TAKEN 0.050 0.056 NOT TAKEN NOT TAKEN NOT TAKEN 0.042 0.042 0.059 NOT TAKEN 0.057 NOT TAKEN 0.052 NOT TAKEN NOT TAKEN 0.043 0.046 NOT TAKEN NOT TAKEN 0.042 SPILLED 0.032 0.037 0.017 NOT TAKEN NOT TAKEN 0.037 0.057 0.041 NOT TAKEN 0.041 NOT TAKEN 0.042 0.055 NOT TAKEN NOT TAKEN NOT TAKEN 0.038 0.039 NOT TAKEN NOT TAKEN 0.051 NOT TAKEN 0.045 NOT TAKEN NOT TAKEN 0.043 NOT TAKEN NOT TAKEN NOT TAKEN 0.034 0.054 0.033 0.001 0.026 NOT TAKEN NOT TAKEN 0.017 SPILLED 0.041 NOT TAKEN 0.042 NOT TAKEN 0.041 0.054 NOT TAKEN NOT TAKEN NOT TAKEN 0.046 0.037 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN 0.057 NOT TAKEN NOT TAKEN 0.040 NOT TAKEN NOT TAKEN NOT TAKEN 0.035 SPILLED 0.030 NOT TAKEN 0.029 NOT TAKEN NOT TAKEN 0.035 0.049 0.044 NOT TAKEN 0.044 NOT TAKEN 0.039 0.056 NOT TAKEN NOT TAKEN NOT TAKEN 0.040 0.038 NOT TAKEN NOT TAKEN 0.046 NOT TAKEN 0.053 NOT TAKEN NOT TAKEN 0.038 NOT TAKEN NOT TAKEN NOT TAKEN 0.032 0.044 0.028 NOT TAKEN 0.034 NOT TAKEN NOT TAKEN 0.035 0.047 0.036 NOT TAKEN 0.042 NOT TAKEN 0.034 0.058 NOT TAKEN NOT TAKEN NOT TAKEN 0.039 0.039 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.048 NOT TAKEN NOT TAKEN 0.037 NOT TAKEN NOT TAKEN NOT TAKEN 0.032 0.042 0.024 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.013 0.033 NOT TAKEN 0.041 NOT TAKEN 0.030 0.053 NOT TAKEN NOT TAKEN NOT TAKEN 0.040 0.036 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.036 NOT TAKEN NOT TAKEN NOT TAKEN 0.030 0.051 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.032 NOT TAKEN 0.047 NOT TAKEN NOT TAKEN 0.069 NOT TAKEN NOT TAKEN NOT TAKEN 0.039 0.035 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.041 NOT TAKEN NOT TAKEN NOT TAKEN 0.026 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.029 NOT TAKEN 0.047 NOT TAKEN NOT TAKEN 0.045 NOT TAKEN NOT TAKEN NOT TAKEN 0.042 0.039 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.041 NOT TAKEN NOT TAKEN NOT TAKEN 0.025 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 4103 Covance 6329-212 Appendix 4 Covance 6329-228 3M T-6316.1 Individual Test Material Consumption Data (mg/kg/day) PAGE: 20 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 9 - 1 PPM C92452 C92453 C92454 C92455 C92456 C92457 C92458 C92459 C92460 C92461 C92462 C92463 C92464 C92465 C92466 C92467 C92468 C92469 C92470 0.057 0.045 0.040 0.044 SPILLED 0.051 NOT TAKEN 0.056 0.047 NOT TAKEN 0.051 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.037 0.034 0.042 0.038 0.029 NOT TAKEN 0.050 0.043 NOT TAKEN 0.047 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.046 0.034 NOT TAKEN 0.038 0.038 0.037 NOT TAKEN 0.046 0.027 NOT TAKEN 0.039 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.052 0.030 NOT TAKEN 0.039 0.035 NOT TAKEN NOT TAKEN 0.042 0.040 NOT TAKEN 0.035 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.047 0.021 NOT TAKEN 0.036 0.037 NOT TAKEN NOT TAKEN 0.032 0.023 NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.055 0.020 NOT TAKEN 0.036 0.036 NOT TAKEN NOT TAKEN 0.032 SPILLED NOT TAKEN 0.014 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 0.015 0.035 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.040 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4104 APPENDIX 5 Individual Clinical Hematology Data Individual Clinical Chemistry Data Individual Clinical Urinalysis Data Individual Urine Chemistry Data Covance 6329-212 Covance 6329-228 3M T-6316.1 4105 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 9.14 9.40 8.68 8.44 8.31 7.37 8.34 6.49 8.45 8.58 16.1 16.1 15.3 15.4 15.8 13.9 14.6 12.5 16.2 15.6 46.2 46.1 44.6 46.0 45.9 40.7 43.6 36.8 47.4 45.8 50.5 49.1 51.4 54.5 55.2 55.2 52.3 56.7 56.2 53.4 17.6 17.1 17.6 18.3 19.0 18.9 17.5 19.3 19.2 18.1 34.8 34.8 34.2 33.6 34.4 34.2 33.4 34.1 34.3 34.0 1317 1003 1053 1019 1104 1019 1168 1128 1083 1042 MEAN S.D. N 8.32 .838 10 15.2 1.18 10 44.3 3.23 10 53.4 2.55 10 18.3 .80 10 34.2 .45 10 1094 94.6 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 8.72 8.33 7.75 8.57 8.33 8.09 7.98 8.31 9.21 8.55 15.8 14.6 14.4 14.7 15.1 14.5 14.1 15.6 15.9 15.5 46.1 43.1 42.4 44.0 44.2 42.6 41.6 45.0 46.2 45.5 52.8 51.8 54.7 51.3 53.1 52.6 52.2 54.2 50.1 53.2 18.1 17.6 18.6 17.1 18.1 17.9 17.7 18.7 17.2 18.2 34.2 33.9 34.0 33.4 34.1 34.0 33.9 34.6 34.3 34.1 1063 1217 1053 1104 1134 1078 1045 962 1018 1262 MEAN S.D. N 8.38 .411 10 15.0 .64 10 44.1 1.62 10 52.6 1.35 10 17.9 .53 10 34.0 .31 10 1094 90.4 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4106 Covance 6329-212 ANIMAL NUMBER ------ Group: 8 C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 MEAN S.D. N Group: 9 C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 MEAN S.D. N Appendix 5 Covance 6329-228 3M T-6316.1 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 0 Dosage Unit: ppm 14.1 1.6 11.0 1.3 .2 .0 12 78 9 2 0 8.3 1.2 6.2 .7 .1 .0 15 75 9 1 0 10.0 1.0 7.6 1.0 .4 .0 10 76 10 4 0 10.7 1.4 8.4 .7 .2 .0 13 78 6 2 0 8.5 1.0 6.6 .6 .2 .0 12 78 7 2 0 8.4 1.3 5.8 1.1 .2 .0 15 69 13 2 0 12.3 1.9 9.3 .8 .3 .0 15 76 6 3 0 10.8 1.2 8.3 .8 .5 .0 11 77 8 5 0 12.9 1.1 10.9 .7 .2 .0 8 85 6 1 0 7.8 1.2 5.2 1.1 .3 .0 16 66 14 4 0 10.4 1.3 7.9 .9 .3 .0 13 76 9 3 0 2.18 .28 2.04 .23 .12 .00 2.6 5.2 2.9 1.3 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 1 Dosage Unit: ppm 9.3 1.7 6.5 .8 .2 .0 18 70 9 3 0 8.1 1.0 6.1 .9 .2 .0 12 75 10 2 0 11.3 1.1 9.2 .9 .2 .0 9 81 8 1 0 10.6 1.6 7.6 .9 .4 .0 16 72 9 3 0 9.0 .9 7.1 .9 .1 .0 10 78 10 1 0 11.4 .9 8.0 2.4 .1 .0 8 70 21 1 0 10.9 1.1 8.5 .9 .3 .1 10 78 9 2 0 6.6 1.1 4.5 .8 .1 .0 17 68 13 2 0 10.0 2.0 7.0 .8 .3 .0 19 69 8 3 0 14.4 1.6 10.7 1.4 .7 .0 11 74 10 5 0 10.2 1.3 7.5 1.1 .3 .0 13 74 11 2 0 2.12 .39 1.72 .50 .18 .03 4.1 4.4 3.9 1.3 .0 10 10 10 10 10 10 10 10 10 10 10 4107 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92191 - - - - - C92193 - - - - - C92194 - - - - - C92195 - - - - - C92197 - - - - - C92199 - - - - - C92200 - - - - - C92201 - - - - - C92202 - - - - - C92203 - - - - - Group: 9 Dose Level: 1 Dosage Unit: ppm C92261 - - - - - C92262 - - - - - C92263 - - - - - C92265 - - - - - C92266 - - - - - C92267 - - - - - C92268 - - - - - C92269 - - - - - C92270 - - - - - C92271 - - - - - Covance 6329-212 Covance 6329-228 3M T-6316.1 4108 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 7.70 7.89 8.25 7.93 8.19 8.17 7.84 7.63 7.09 7.49 14.5 15.0 15.8 15.3 15.5 14.7 14.5 14.6 13.7 13.5 42.4 43.8 46.0 44.4 45.5 43.3 42.7 43.2 39.1 40.5 55.1 55.5 55.8 56.0 55.6 53.0 54.4 56.6 55.2 54.1 18.9 19.1 19.1 19.3 19.0 17.9 18.4 19.2 19.3 18.0 34.3 34.3 34.3 34.5 34.1 33.9 33.9 33.9 35.0 33.3 946 1100 1022 1017 1007 1056 1123 990 1249 1130 MEAN S.D. N 7.82 .358 10 14.7 .73 10 43.1 2.10 10 55.1 1.05 10 18.8 .53 10 34.2 .45 10 1064 88.2 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 7.61 8.12 7.61 8.64 8.56 7.79 7.99 7.93 7.89 7.95 15.2 15.2 15.0 16.1 15.6 14.7 15.2 15.2 15.1 14.9 44.3 44.8 42.7 47.4 45.6 43.0 44.1 42.7 44.1 43.8 58.2 55.1 56.1 54.8 53.3 55.2 55.2 53.9 55.9 55.1 20.0 18.7 19.7 18.6 18.2 18.9 19.0 19.1 19.1 18.7 34.3 33.9 35.1 33.9 34.1 34.3 34.4 35.5 34.3 34.0 1022 1096 1176 1136 1122 1114 1267 958 1080 1144 MEAN S.D. N 8.01 .350 10 15.2 .39 10 44.2 1.44 10 55.3 1.32 10 19.0 .53 10 34.4 .52 10 1112 83.6 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4109 Covance 6329-212 ANIMAL NUMBER ------ Group: 8 C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 MEAN S.D. N Group: 9 C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 MEAN S.D. N Appendix 5 Covance 6329-228 3M T-6316.1 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 0 Dosage Unit: ppm 7.1 .6 5.7 .5 .3 .0 9 80 7 4 0 6.4 .7 4.9 .7 .2 .0 10 75 10 3 0 9.7 1.8 7.1 .6 .2 .0 18 73 6 2 0 4.8 .5 3.8 .4 .1 .0 10 78 8 3 0 8.0 .7 6.5 .6 .2 .0 8 82 7 2 0 5.1 .5 4.2 .3 .1 .0 10 82 6 3 0 12.0 1.0 10.0 .6 .4 .0 9 83 5 3 0 4.8 .9 3.5 .2 .1 .0 20 73 5 3 0 10.4 1.0 8.5 .6 .2 .0 10 82 6 2 0 5.0 .8 3.5 .5 .2 .0 16 70 9 5 0 7.3 .8 5.8 .5 .2 .0 12 78 7 3 0 2.61 .38 2.24 .16 .09 .00 4.3 4.7 1.7 .9 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 1 Dosage Unit: ppm 5.1 .9 3.7 .4 .2 .0 17 72 8 3 0 7.3 1.0 5.8 .4 .2 .0 13 79 5 3 0 6.0 1.1 4.2 .5 .2 .0 18 71 8 3 0 8.9 .8 7.5 .4 .1 .0 9 84 5 1 0 7.4 .8 5.8 .6 .2 .0 11 77 8 3 0 6.5 1.1 5.0 .2 .1 .0 17 77 3 2 0 5.1 1.3 2.7 .8 .3 .0 25 53 16 5 0 6.7 .6 5.5 .4 .2 .0 8 83 6 3 0 7.0 1.0 5.5 .3 .2 .0 14 78 4 3 0 5.1 .8 3.9 .3 .1 .0 16 76 5 3 0 6.5 .9 5.0 .4 .2 .0 15 75 7 3 0 1.23 .20 1.37 .17 .06 .00 5.0 8.7 3.7 1.0 .0 10 10 10 10 10 10 10 10 10 10 10 4110 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92331 - - - - - C92333 - - - - - C92334 - - - - - C92336 - - - - - C92337 - - - - - C92338 - - - - - C92339 - - - - - C92340 - - - - - C92341 - - - - - C92342 - - - - - Group: 9 Dose Level: 1 Dosage Unit: ppm C92401 - - - - - C92403 - - - - - C92404 - - - - - C92405 - - - - - C92406 - - - - - C92407 - - - - - C92408 - - - - - C92409 - - - - - C92410 - - - - - C92411 - - - - - Covance 6329-212 Covance 6329-228 3M T-6316.1 4111 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 8.28 8.69 8.58 8.44 8.25 7.44 7.85 7.70 8.71 8.30 14.9 15.4 15.2 15.6 15.5 14.3 14.6 14.8 16.7 14.9 44.2 45.4 44.1 45.5 45.1 41.5 43.4 43.2 49.1 44.8 53.4 52.3 51.4 53.9 54.7 55.8 55.2 56.1 56.3 54.0 17.9 17.7 17.7 18.5 18.8 19.2 18.6 19.2 19.2 18.0 33.6 33.9 34.4 34.3 34.4 34.3 33.7 34.1 34.1 33.4 1298 918 1131 948 1147 1070 1002 1129 1103 1081 MEAN S.D. N 8.22 .430 10 15.2 .67 10 44.6 1.98 10 54.3 1.63 10 18.5 .62 10 34.0 .36 10 1083 109.2 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 9.00 7.70 8.01 8.61 8.51 8.03 7.54 8.21 8.93 8.21 16.0 14.0 14.9 14.5 15.1 14.3 13.5 15.3 15.8 14.9 46.8 41.0 43.9 43.6 45.4 42.6 40.2 45.1 46.6 43.9 52.0 53.2 54.8 50.6 53.4 53.1 53.4 54.9 52.2 53.5 17.8 18.2 18.6 16.8 17.7 17.8 17.8 18.7 17.7 18.2 34.3 34.2 33.9 33.3 33.2 33.6 33.4 34.0 34.0 34.0 1027 1179 1137 1191 1091 1092 1004 992 1187 1218 MEAN S.D. N 8.28 .487 10 14.8 .78 10 43.9 2.19 10 53.1 1.28 10 17.9 .54 10 33.8 .39 10 1112 83.2 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4112 Covance 6329-212 ANIMAL NUMBER ------ Group: 8 C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 MEAN S.D. N Group: 9 C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 MEAN S.D. N Appendix 5 Covance 6329-228 3M T-6316.1 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 0 Dosage Unit: ppm 11.3 2.0 7.6 1.2 .3 .0 18 68 11 3 0 6.5 1.1 5.2 .1 .1 .0 18 80 1 1 0 9.8 1.7 6.7 1.0 .4 .0 18 68 10 4 0 8.1 1.1 6.0 .8 .1 .0 14 74 10 2 1 7.4 1.4 4.9 .9 .2 .0 18 67 12 2 0 7.2 1.0 5.0 1.0 .1 .0 14 70 14 1 1 9.5 1.8 6.0 1.3 .4 .1 18 64 14 4 1 8.6 1.6 5.6 1.0 .3 .0 19 65 12 4 0 11.5 3.8 6.2 1.3 .2 .0 33 54 11 2 0 6.4 1.5 3.5 1.0 .4 .1 23 54 16 6 1 8.6 1.7 5.7 1.0 .2 .0 19 66 11 3 0 1.85 .81 1.12 .34 .13 .04 5.4 8.0 4.0 1.6 .5 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 1 Dosage Unit: ppm 6.9 1.5 4.2 .9 .2 .0 22 61 14 3 0 8.2 1.6 5.2 1.2 .2 .0 19 64 15 2 0 8.4 1.1 6.2 .9 .1 .1 13 74 10 2 1 9.5 1.8 6.2 1.2 .3 .1 18 65 13 4 1 9.1 1.2 6.9 .9 .1 .0 13 76 10 1 0 10.6 1.4 5.6 3.1 .1 .4 13 53 30 1 3 8.7 1.2 6.3 .9 .2 .1 14 73 10 2 1 6.8 1.6 3.9 1.2 .1 .0 23 58 17 1 1 10.2 2.0 7.0 .9 .3 .0 19 69 9 3 0 10.3 1.4 7.4 1.2 .2 .0 13 72 12 2 0 8.9 1.5 5.9 1.2 .2 .1 17 66 14 2 1 1.34 .28 1.17 .67 .08 .13 4.0 7.6 6.2 1.0 .9 10 10 10 10 10 10 10 10 10 10 10 4113 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92191 - - - - - C92193 - - - - - C92194 - - - - - C92195 - - - - - C92197 - - - - - C92199 - - - - - C92200 - - - - - C92201 - - - - - C92202 - - - - - C92203 - - - - - Group: 9 Dose Level: 1 Dosage Unit: ppm C92261 - - - - - C92262 - - - - - C92263 - - - - - C92265 - - - - - C92266 - - - - - C92267 - - - - - C92268 - - - - - C92269 - - - - - C92270 - - - - - C92271 - - - - - Covance 6329-212 Covance 6329-228 3M T-6316.1 4114 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 7.54 7.46 7.71 7.31 7.36 7.50 7.23 6.89 7.22 6.89 14.5 14.9 14.7 14.2 14.1 13.8 13.0 13.6 14.1 12.5 41.5 42.5 42.8 42.0 41.1 40.6 38.7 39.7 40.3 37.0 55.1 57.0 55.5 57.4 55.9 54.2 53.6 57.6 55.8 53.7 19.2 19.9 19.0 19.5 19.1 18.4 18.0 19.7 19.6 18.2 34.9 35.0 34.2 33.9 34.2 34.0 33.7 34.2 35.1 33.9 993 1054 1018 941 931 993 1201 967 1223 1061 MEAN S.D. N 7.31 .267 10 13.9 .75 10 40.6 1.79 10 55.6 1.46 10 19.1 .66 10 34.3 .50 10 1038 101.1 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 7.15 7.41 7.36 8.00 8.18 7.29 8.41 7.91 7.28 7.29 14.5 14.3 14.6 15.2 15.2 14.1 15.4 15.3 14.4 13.9 41.7 41.1 42.1 44.0 44.9 40.8 44.7 45.0 40.8 40.1 58.3 55.4 57.2 55.0 54.9 55.9 53.1 56.9 56.0 55.1 20.2 19.4 19.9 19.0 18.6 19.3 18.3 19.3 19.8 19.1 34.6 34.9 34.7 34.5 33.9 34.5 34.4 34.0 35.3 34.7 927 1019 1032 1061 1125 982 1472 963 1184 1210 MEAN S.D. N 7.63 .451 10 14.7 .54 10 42.5 1.93 10 55.8 1.45 10 19.3 .58 10 34.6 .41 10 1098 161.0 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4115 Covance 6329-212 ANIMAL NUMBER ------ Group: 8 C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 MEAN S.D. N Group: 9 C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 MEAN S.D. N Appendix 5 Covance 6329-228 3M T-6316.1 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 0 Dosage Unit: ppm 6.6 .7 4.9 .7 .2 .0 11 75 11 2 1 4.6 .7 3.1 .5 .2 .0 16 68 12 4 1 7.3 .8 5.9 .5 .1 .0 10 81 7 2 0 5.1 .5 3.9 .6 .1 .0 11 76 11 2 0 7.4 .8 6.0 .4 .1 .0 11 80 6 2 0 4.8 .6 3.7 .4 .1 .0 13 76 9 1 1 12.1 2.9 7.3 1.3 .6 .0 24 60 11 5 0 3.9 .7 2.8 .3 .1 .0 18 72 7 2 0 6.4 .8 5.0 .4 .1 .0 13 79 6 2 0 5.8 1.5 3.5 .6 .2 .0 26 60 10 3 1 6.4 1.0 4.6 .6 .2 .0 15 73 9 2 0 2.32 .72 1.46 .28 .15 .00 5.7 7.7 2.3 1.2 .5 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 1 Dosage Unit: ppm 4.4 .7 3.3 .2 .1 .0 16 75 6 3 0 6.3 .9 4.7 .5 .1 .0 14 75 8 2 1 5.4 .8 4.0 .4 .1 .0 15 75 8 2 0 6.8 .8 5.4 .5 .1 .0 12 80 7 1 0 10.4 1.5 7.8 .9 .2 .0 15 75 8 2 0 4.0 .6 3.1 .3 .1 .0 14 78 6 2 0 4.4 1.4 2.0 .8 .2 .0 32 46 18 4 0 6.6 .8 5.2 .5 .1 .0 12 78 7 2 0 4.6 .7 3.4 .4 .1 .0 16 73 8 3 0 5.0 .9 3.4 .6 .1 .0 18 67 12 2 0 5.8 .9 4.2 .5 .1 .0 16 72 9 2 0 1.90 .30 1.63 .21 .04 .00 5.8 9.9 3.6 .8 .3 10 10 10 10 10 10 10 10 10 10 10 4116 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92331 - - - - - C92333 - - - - - C92334 - - - - - C92336 - - - - - C92337 - - - - - C92338 - - - - - C92339 - - - - - C92340 - - - - - C92341 - - - - - C92342 - - - - - Group: 9 Dose Level: 1 Dosage Unit: ppm C92401 - - - - - C92403 - - - - - C92404 - - - - - C92405 - - - - - C92406 - - - - - C92407 - - - - - C92408 - - - - - C92409 - - - - - C92410 - - - - - C92411 - - - - - Covance 6329-212 Covance 6329-228 3M T-6316.1 4117 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92195 C92199 C92205 C92211 C92223 C92230 C92238 C92246 C92247 C92253 8.74 7.46 8.55 7.98 7.57 9.11 7.88 8.43 7.89 8.40 16.2 14.3 15.7 13.9 14.4 15.7 15.2 15.4 14.8 16.0 45.4 40.2 44.8 39.2 40.1 44.0 42.2 43.3 41.9 44.8 51.9 53.9 52.4 49.2 53.0 48.3 53.5 51.3 53.1 53.4 18.5 19.1 18.4 17.5 19.1 17.2 19.2 18.3 18.7 19.0 35.6 35.5 35.2 35.5 36.0 35.6 35.9 35.6 35.3 35.6 928 1127 1110 1152 1459 1125 1292 1214 1324 978 MEAN S.D. N 8.20 .530 10 15.2 .78 10 42.6 2.22 10 52.0 1.89 10 18.5 .69 10 35.6 .24 10 1171 158.9 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92267 C92274 C92286 C92287 C92288 C92303 C92315 C92317 C92320 C92324 8.08 9.19 8.29 8.72 8.34 8.98 8.07 7.95 8.61 7.95 14.9 15.5 15.1 15.3 15.9 14.9 14.8 13.9 16.5 15.7 42.5 45.0 42.2 43.4 44.8 42.9 42.1 39.7 46.9 44.0 52.6 48.9 51.0 49.7 53.7 47.7 52.2 49.9 54.5 55.4 18.5 16.9 18.2 17.5 19.0 16.5 18.4 17.5 19.1 19.8 35.1 34.6 35.7 35.3 35.4 34.6 35.2 35.1 35.2 35.7 1060 1310 1176 1207 1075 1278 899 1450 941 911 MEAN S.D. N 8.42 .439 10 15.2 .71 10 43.4 1.97 10 51.6 2.54 10 18.1 1.04 10 35.2 .38 10 1131 185.5 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4118 Covance 6329-212 ANIMAL NUMBER ------ Group: 8 C92195 C92199 C92205 C92211 C92223 C92230 C92238 C92246 C92247 C92253 MEAN S.D. N Group: 9 C92267 C92274 C92286 C92287 C92288 C92303 C92315 C92317 C92320 C92324 MEAN S.D. N Appendix 5 Covance 6329-228 3M T-6316.1 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 0 Dosage Unit: ppm 5.2 2.1 2.6 .4 .1 .0 40 50 7 2 0 8.7 3.7 3.6 1.2 .1 .0 42 42 14 1 0 4.8 1.0 3.4 .3 .1 .0 22 71 6 1 0 6.4 2.1 2.8 1.3 .1 .0 34 44 21 2 1 8.9 2.0 5.8 .8 .2 .0 23 66 9 3 0 6.5 1.5 4.0 .9 .1 .1 24 60 14 1 1 6.9 1.5 4.1 1.1 .1 .0 22 59 16 2 0 5.7 2.3 2.8 .5 .2 .0 40 48 9 3 0 9.5 2.0 6.5 .7 .1 .0 21 69 8 1 0 5.8 1.5 3.6 .6 .2 .0 26 61 10 3 0 6.8 2.0 3.9 .8 .1 .0 29 57 11 2 0 1.64 .73 1.29 .34 .05 .03 8.6 10.4 4.7 .9 .4 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 1 Dosage Unit: ppm 5.5 1.2 3.4 .8 .1 .0 22 61 15 1 0 5.5 1.8 3.0 .6 .1 .0 32 55 11 2 0 7.1 2.1 3.8 1.1 .1 .0 29 54 15 2 0 5.2 1.7 3.0 .4 .1 .0 34 57 7 2 0 6.4 1.8 3.8 .7 .2 .0 27 59 11 2 0 11.4 5.1 4.8 1.4 .1 .0 45 42 12 1 0 5.1 1.1 3.3 .6 .1 .0 21 65 11 2 0 9.5 2.4 5.9 1.0 .2 .0 25 63 10 2 0 5.7 1.6 3.0 .9 .2 .0 28 53 15 3 0 5.5 1.1 3.5 .8 .1 .0 21 63 15 2 0 6.7 2.0 3.8 .8 .1 .0 28 57 12 2 0 2.12 1.17 .93 .29 .05 .00 7.3 6.7 2.7 .6 .0 10 10 10 10 10 10 10 10 10 10 10 4119 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92195 - - - - - C92199 - - - - - C92205 - - - - - C92211 - - - - - C92223 - - - - - C92230 - - - - - C92238 - - - - - C92246 - - - - - C92247 - - - - - C92253 - - - - - Group: 9 Dose Level: 1 Dosage Unit: ppm C92267 - - - - - C92274 - - - - - C92286 - - - - - C92287 - - - - - C92288 - - - - - C92303 - - - - - C92315 - - - - - C92317 - - - - - C92320 - - - - - C92324 - - - - - Covance 6329-212 Covance 6329-228 3M T-6316.1 4120 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92341 C92356 C92357 C92365 C92372 C92376 C92388 C92389 C92390 C92395 8.10 7.25 8.08 7.20 7.48 7.57 8.20 7.69 8.00 7.57 15.7 14.1 15.8 15.1 14.6 14.2 15.3 14.9 15.1 14.5 43.3 38.6 43.4 41.9 40.0 39.4 43.6 41.4 42.3 40.5 53.5 53.2 53.7 58.2 53.5 52.1 53.2 53.8 52.9 53.5 19.4 19.4 19.6 21.0 19.6 18.8 18.6 19.3 18.9 19.1 36.3 36.5 36.5 36.1 36.6 36.0 35.1 35.9 35.8 35.7 709 1047 1097 915 929 855 870 1069 920 1003 MEAN S.D. N 7.71 .362 10 14.9 .58 10 41.4 1.77 10 53.8 1.63 10 19.4 .67 10 36.0 .46 10 941 117.1 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92406 C92407 C92411 C92418 C92422 C92424 C92433 C92438 C92441 C92449 8.50 7.55 7.97 7.58 8.45 7.23 7.95 7.63 7.29 7.66 15.5 14.7 15.0 14.7 15.5 14.3 15.5 14.4 14.6 14.7 43.0 41.1 41.4 40.7 43.7 40.0 42.8 41.1 41.3 41.0 50.6 54.4 52.0 53.6 51.7 55.2 53.9 53.8 56.6 53.5 18.2 19.5 18.8 19.4 18.3 19.7 19.5 18.8 20.1 19.2 36.0 35.8 36.1 36.2 35.4 35.7 36.1 34.9 35.4 35.8 1065 1022 1276 1057 1104 1022 939 918 999 1130 MEAN S.D. N 7.78 .435 10 14.9 .46 10 41.6 1.16 10 53.5 1.75 10 19.2 .61 10 35.7 .41 10 1053 102.5 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4121 Covance 6329-212 ANIMAL NUMBER ------ Group: 8 C92341 C92356 C92357 C92365 C92372 C92376 C92388 C92389 C92390 C92395 MEAN S.D. N Group: 9 C92406 C92407 C92411 C92418 C92422 C92424 C92433 C92438 C92441 C92449 MEAN S.D. N Appendix 5 Covance 6329-228 3M T-6316.1 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 0 Dosage Unit: ppm 6.6 1.6 4.4 .5 .1 .0 25 66 7 1 0 7.2 2.3 4.1 .7 .1 .0 32 57 10 1 0 5.9 1.9 3.1 .8 .1 .0 32 52 13 2 0 4.4 1.1 2.7 .5 .1 .0 25 62 11 2 0 6.1 1.5 3.8 .7 .2 .0 24 62 12 3 0 3.3 1.0 1.8 .4 .1 .0 29 56 12 3 0 3.2 .6 2.1 .4 .1 .0 20 65 14 2 0 3.6 1.2 1.9 .5 .1 .0 32 53 13 2 0 7.6 1.6 5.2 .6 .1 .0 21 69 8 1 0 4.5 1.4 2.5 .5 .1 .0 31 56 10 3 0 5.2 1.4 3.2 .6 .1 .0 27 60 11 2 0 1.64 .48 1.16 .13 .03 .00 4.7 5.8 2.3 .8 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 1 Dosage Unit: ppm 6.4 2.6 2.9 .7 .1 .0 41 45 12 2 0 3.5 1.1 2.1 .2 .1 .0 32 61 5 2 0 5.6 1.3 3.3 .8 .1 .0 23 59 15 2 0 2.5 .6 1.2 .5 .1 .0 26 49 22 2 1 2.9 .6 1.8 .4 .1 .0 21 62 13 4 0 3.7 1.0 2.3 .3 .1 .0 27 62 9 2 0 5.2 .8 3.8 .4 .1 .0 16 73 9 2 0 9.3 1.1 7.1 1.0 .2 .1 12 76 10 2 1 7.7 2.6 4.2 .6 .2 .0 34 55 8 3 0 4.4 1.0 2.8 .6 .1 .0 23 63 12 2 0 5.1 1.3 3.2 .6 .1 .0 26 60 12 2 0 2.18 .73 1.66 .24 .04 .03 8.6 9.5 4.6 .7 .4 10 10 10 10 10 10 10 10 10 10 10 4122 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92341 - - - - - C92356 - - - - - C92357 - - - - - C92365 - - - - - C92372 - - - - - C92376 - - - - - C92388 - - - - - C92389 - - - - - C92390 - - - - - C92395 - - - - - Group: 9 Dose Level: 1 Dosage Unit: ppm C92406 - - - - - C92407 - - - - - C92411 - - - - - C92418 - - - - - C92422 - - - - - C92424 - - - - - C92433 - - - - - C92438 - - - - - C92441 - - - - - C92449 - - - - - Covance 6329-212 Covance 6329-228 3M T-6316.1 4123 Appendix 5 Individual Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ PCOAO IU/G -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92192 11 C92196 8 C92207 8 C92216 4 C92259 6 MEAN 7 S.D. 2.6 N 5 Group: 9 Dose Level: 1 Dosage Unit: ppm C92264 6 C92280 2 C92284 4 C92300 10 C92329 11 MEAN 7 S.D. 3.8 N 5 Covance 6329-212 Covance 6329-228 3M T-6316.1 4124 Appendix 5 Individual Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ PCOAO IU/G -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92332 2 C92352 1 C92355 4 C92366 3 C92373 3 MEAN 3 S.D. 1.1 N 5 Group: 9 Dose Level: 1 Dosage Unit: ppm C92402 5 C92435 0 C92463 5 C92467 6 C92470 4 MEAN 4 S.D. 2.3 N 5 Covance 6329-212 Covance 6329-228 3M T-6316.1 4125 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 107 13 .7 7.3 4.9 2.4 .1 48 108 11 .8 7.4 5.2 2.2 .0 52 126 14 .7 6.6 4.6 2.0 .0 57 108 11 .6 6.2 4.7 1.5 .0 59 109 14 .6 6.8 4.6 2.2 .0 66 111 16 .5 6.4 4.5 1.9 .1 56 130 18 .7 7.3 5.0 2.3 .1 64 124 14 .6 7.0 4.6 2.4 .0 64 102 13 .8 7.1 4.8 2.3 .0 69 108 13 .6 6.8 4.7 2.1 .0 51 MEAN S.D. N 113 14 .7 6.9 4.8 2.1 .0 59 9.6 2.1 .10 .40 .22 .28 .05 7.0 10 10 10 10 10 10 10 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 109 12 .6 7.0 4.7 2.3 .0 48 118 14 .6 7.1 4.7 2.4 .1 56 109 18 .5 6.8 4.7 2.1 .1 48 110 14 .7 7.4 4.6 2.8 .0 77 113 12 .7 6.9 4.8 2.1 .1 56 100 15 .7 6.7 4.3 2.4 .0 50 121 11 .7 7.1 4.9 2.2 .0 78 126 12 .4 7.4 4.9 2.5 .1 70 115 13 .5 6.4 4.5 1.9 .0 75 119 11 .6 7.3 4.8 2.5 .1 75 MEAN S.D. N 114 13 .6 7.0 4.7 2.3 .0 63 7.4 2.1 .11 .32 .19 .26 .05 12.8 10 10 10 10 10 10 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4126 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 101 34 162 38 99 32 103 38 126 36 85 40 105 58 115 32 95 32 105 46 2 10.6 7.1 147 6.0 106 7 10.4 7.2 148 5.5 108 2 10.4 7.6 147 6.4 107 1 10.5 6.3 146 5.5 107 4 10.5 7.2 147 5.9 107 3 10.8 8.3 145 5.5 104 0 10.8 8.2 146 6.6 102 1 10.2 6.6 147 5.8 108 1 10.7 6.9 147 6.2 106 0 10.6 7.2 146 5.8 107 MEAN S.D. N 110 21.4 10 39 8.1 10 2 2.1 10 10.6 .19 10 7.3 .63 10 147 .8 10 5.9 .39 10 106 1.9 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 136 57 131 101 90 31 99 30 113 36 112 39 118 39 94 61 109 42 105 43 5 10.4 6.6 146 6.0 107 2 10.6 7.3 146 6.3 108 3 10.5 7.2 146 6.3 107 7 10.8 7.1 147 6.4 107 0 10.7 7.4 146 6.8 106 2 10.3 6.8 147 6.3 108 1 10.8 6.9 146 6.0 107 0 10.4 6.7 148 5.7 107 3 10.2 6.7 147 6.2 108 3 10.9 7.4 148 6.6 107 MEAN S.D. N 111 14.9 10 48 21.2 10 3 2.2 10 10.6 .24 10 7.0 .31 10 147 .8 10 6.3 .31 10 107 .6 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4127 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 126 17 .8 7.5 5.7 1.8 .1 64 105 14 .9 7.8 5.8 2.0 .0 80 118 16 .9 8.5 6.1 2.4 .2 96 120 14 1.0 8.6 6.1 2.5 .3 109 113 14 .8 7.3 5.3 2.0 .0 79 123 18 .7 8.1 6.0 2.1 .1 93 105 11 .7 8.0 6.0 2.0 .0 78 118 12 .8 8.8 6.6 2.2 .3 88 117 14 .7 8.5 6.6 1.9 .5 115 99 12 .5 9.2 7.1 2.1 .1 140 MEAN S.D. N 114 14 .8 8.2 6.1 2.1 .2 94 8.7 2.3 .14 .60 .52 .22 .16 22.1 10 10 10 10 10 10 10 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 114 11 .8 7.4 5.6 1.8 .3 68 114 13 .8 8.1 6.5 1.6 .2 90 110 18 .6 9.4 7.1 2.3 .3 138 104 17 .7 8.1 5.6 2.5 .1 87 111 18 .7 8.1 5.8 2.3 .0 97 104 14 .8 8.6 6.3 2.3 .0 84 107 16 .8 7.4 5.3 2.1 .0 94 112 13 .9 7.2 5.0 2.2 .1 76 110 17 .5 7.8 5.9 1.9 .3 65 113 14 .8 7.3 5.1 2.2 .0 86 MEAN S.D. N 110 15 .7 7.9 5.8 2.1 .1 88 3.8 2.4 .12 .68 .66 .27 .13 20.3 10 10 10 10 10 10 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4128 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 83 42 88 35 91 34 98 30 94 37 85 27 108 37 80 35 275 112 98 55 2 10.9 6.1 146 5.3 108 2 11.3 6.4 145 6.0 106 2 11.9 6.5 149 6.6 106 1 11.6 5.8 147 6.1 108 4 10.6 5.5 144 6.0 106 3 11.1 5.4 144 5.6 107 4 11.3 6.7 144 5.8 104 3 11.4 5.6 145 5.5 106 0 11.7 6.2 145 5.5 103 1 12.5 5.9 144 5.8 103 MEAN S.D. N 110 58.6 10 44 24.9 10 2 1.3 10 11.4 .54 10 6.0 .44 10 145 1.6 10 5.8 .38 10 106 1.8 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 104 34 103 36 99 43 113 48 157 83 82 29 140 74 83 27 104 36 90 34 2 11.0 6.3 147 5.2 106 4 11.3 5.9 146 5.6 105 0 11.4 6.6 146 5.7 103 1 11.3 7.2 148 6.3 107 0 10.9 6.2 146 5.8 107 1 11.2 5.9 146 6.1 106 6 11.7 6.8 148 6.2 109 1 10.7 6.1 146 6.3 111 0 11.3 6.6 147 6.2 107 0 10.9 6.8 147 6.1 108 MEAN S.D. N 108 24.1 10 44 19.1 10 2 2.0 10 11.2 .29 10 6.4 .43 10 147 .8 10 6.0 .36 10 107 2.2 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4129 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ PCOAO IU/G -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92255 7 C92256 5 C92257 4 C92258 5 C92260 7 MEAN 6 S.D. 1.3 N 5 Group: 9 Dose Level: 1 Dosage Unit: ppm C92325 5 C92326 4 C92327 5 C92328 7 C92330 6 MEAN 5 S.D. 1.1 N 5 Covance 6329-212 Covance 6329-228 3M T-6316.1 4130 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ PCOAO IU/G -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92396 4 C92397 3 C92398 4 C92399 4 C92400 2 MEAN 3 S.D. .9 N 5 Group: 9 Dose Level: 1 Dosage Unit: ppm C92464 4 C92465 6 C92466 7 C92468 3 C92469 4 MEAN 5 S.D. 1.6 N 5 Covance 6329-212 Covance 6329-228 3M T-6316.1 4131 Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 85 12 .7 7.1 4.7 2.4 .2 51 101 13 .6 7.1 5.0 2.1 .1 74 114 11 .6 6.6 4.5 2.1 .2 74 93 12 .6 6.3 4.7 1.6 .1 61 104 11 .6 7.0 4.6 2.4 .1 80 112 12 .5 6.6 4.5 2.1 .1 78 117 14 .7 7.0 4.6 2.4 .2 96 94 13 .6 7.2 4.6 2.6 .1 76 102 11 .7 7.2 4.8 2.4 .1 87 106 10 .6 6.6 4.4 2.2 .1 72 MEAN S.D. N 103 12 .6 6.9 4.6 2.2 .1 75 10.1 1.2 .06 .32 .17 .28 .05 12.5 10 10 10 10 10 10 10 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 112 13 .6 7.1 4.8 2.3 .2 62 107 13 .6 6.9 4.5 2.4 .2 78 102 13 .6 7.1 4.7 2.4 .2 73 103 14 .6 7.4 4.4 3.0 .1 114 107 12 .7 7.2 4.8 2.4 .2 74 105 14 .6 6.5 4.3 2.2 .1 65 121 11 .6 7.1 4.7 2.4 .1 104 120 13 .7 7.6 4.8 2.8 .2 90 119 13 .6 7.0 4.7 2.3 .1 98 116 12 .6 7.2 4.8 2.4 .2 82 MEAN S.D. N 111 13 .6 7.1 4.6 2.5 .2 84 7.3 .9 .04 .29 .18 .25 .05 17.1 10 10 10 10 10 10 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4132 Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 127 40 120 33 156 40 103 36 124 33 84 30 138 109 139 37 95 36 116 39 2 10.5 6.9 148 5.5 103 0 10.5 7.1 147 5.9 108 2 10.3 7.3 145 5.9 106 2 10.6 6.3 145 5.3 104 3 10.8 7.2 147 6.4 106 2 10.7 7.8 146 5.7 105 3 10.6 6.6 145 6.2 102 2 10.5 6.8 147 5.9 102 2 10.8 7.1 148 6.3 104 2 10.4 7.0 147 6.0 106 MEAN S.D. N 120 21.8 10 43 23.3 10 2 .8 10 10.6 .16 10 7.0 .41 10 146 1.2 10 5.9 .34 10 105 2.0 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 113 53 127 80 90 24 109 30 119 39 83 30 113 38 91 68 153 50 105 34 1 10.7 7.4 148 6.5 105 1 10.8 7.5 148 6.2 106 2 10.9 6.8 148 6.2 106 1 10.7 6.7 147 6.5 103 1 10.4 6.3 145 6.1 101 2 10.6 6.5 146 6.3 109 0 10.8 6.7 146 5.9 104 1 10.8 7.4 148 5.8 103 3 11.1 7.6 147 6.5 109 2 11.0 7.3 148 6.7 104 MEAN S.D. N 110 20.4 10 45 18.1 10 1 .8 10 10.8 .20 10 7.0 .47 10 147 1.1 10 6.3 .29 10 105 2.6 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4133 Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 110 16 .7 8.6 6.5 2.1 .2 112 110 14 .7 7.5 5.7 1.8 .2 81 106 16 .7 7.9 5.8 2.1 .2 76 108 13 .7 8.7 6.3 2.4 .2 144 111 12 .7 8.2 6.3 1.9 .2 110 107 16 .7 8.4 6.3 2.1 .2 117 107 12 .7 8.0 5.6 2.4 .2 117 113 12 .7 8.3 6.3 2.0 .2 103 101 13 .7 8.6 6.2 2.4 .2 142 85 13 .6 8.8 6.4 2.4 .2 164 MEAN S.D. N 106 14 .7 8.3 6.1 2.2 8.0 1.7 .03 .41 .32 .23 10 10 10 10 10 10 .2 117 .00 27.5 10 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 108 11 .7 7.4 5.8 1.6 .2 64 110 12 .7 8.4 6.7 1.7 .2 105 101 12 .7 8.7 6.7 2.0 .2 135 92 13 .8 8.5 6.1 2.4 .2 119 102 16 .8 8.5 5.8 2.7 .2 110 97 10 .7 8.5 6.1 2.4 .1 90 110 15 .8 8.1 5.0 3.1 .3 186 114 14 .7 7.5 5.3 2.2 .1 79 106 11 .7 8.6 6.7 1.9 .2 97 96 16 .7 8.1 5.2 2.9 .1 122 MEAN S.D. N 104 13 .7 8.2 5.9 2.3 7.2 2.2 .05 .45 .64 .50 10 10 10 10 10 10 .2 111 .06 33.8 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4134 Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 125 91 68 31 83 31 101 46 78 39 93 30 229 149 91 35 103 46 175 86 1 11.3 5.7 146 4.5 101 3 11.1 6.0 146 6.2 106 2 11.1 4.6 142 5.5 103 2 11.8 6.2 144 5.1 105 0 11.0 5.0 144 5.1 106 2 11.2 5.4 144 5.5 104 7 11.1 6.2 143 5.4 102 1 11.0 5.0 145 4.9 102 2 11.0 6.0 145 4.6 97 1 12.1 6.2 144 5.4 102 MEAN S.D. N 115 50.2 10 58 38.8 10 2 1.9 10 11.3 .38 10 5.6 .59 10 144 1.3 10 5.2 .50 10 103 2.7 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 97 47 109 46 101 36 283 155 244 164 74 27 612 384 103 30 71 32 89 34 2 11.0 6.2 146 5.4 106 1 11.1 5.2 145 5.3 102 2 11.4 5.4 147 5.6 104 3 11.1 5.6 145 4.7 104 3 10.9 6.8 144 4.9 100 2 11.1 5.7 144 5.6 102 22 12.1 6.8 148 6.7 108 2 10.6 6.1 147 5.8 105 2 11.7 6.0 145 5.3 103 2 11.4 6.8 145 5.5 103 MEAN S.D. N 178 168.9 10 96 113.8 10 4 6.3 10 11.2 .43 10 6.1 .59 10 146 1.3 10 5.5 .54 10 104 2.3 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4135 Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92195 C92199 C92205 C92211 C92223 C92230 C92238 C92246 C92247 C92253 94 10 .7 7.1 5.1 2.0 .1 93 105 9 .6 6.9 4.4 2.5 .1 96 105 12 .8 7.4 4.7 2.7 .2 134 106 11 .6 6.6 3.9 2.7 .1 78 148 12 .6 7.2 4.6 2.6 .2 139 140 13 .7 7.6 4.7 2.9 .2 80 129 11 .6 6.6 4.3 2.3 .1 90 106 16 .8 7.5 5.1 2.4 .2 80 145 14 .8 7.1 4.4 2.7 .2 179 116 13 .7 6.9 4.8 2.1 .2 85 MEAN S.D. N 119 12 .7 7.1 4.6 2.5 19.5 2.0 .09 .35 .37 .29 10 10 10 10 10 10 .2 105 .05 33.8 10 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92267 C92274 C92286 C92287 C92288 C92303 C92315 C92317 C92320 C92324 107 13 .7 6.9 4.7 2.2 .1 82 152 12 .7 7.6 4.9 2.7 .1 131 117 8 .6 6.8 4.5 2.3 .1 74 126 12 .7 7.6 4.9 2.7 .2 108 130 13 .7 7.3 4.8 2.5 .2 101 114 15 .7 6.8 3.9 2.9 .1 38 137 14 .7 6.8 4.7 2.1 .2 98 135 11 .7 7.1 4.7 2.4 .2 104 118 8 .7 7.2 4.6 2.6 .2 88 131 12 .7 7.3 4.3 3.0 .1 200 MEAN S.D. N 127 12 .7 7.1 4.6 2.5 13.2 2.3 .03 .31 .31 .30 10 10 10 10 10 10 .2 102 .05 42.1 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4136 Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92195 C92199 C92205 C92211 C92223 C92230 C92238 C92246 C92247 C92253 118 33 66 28 151 65 98 29 83 41 247 414 61 38 86 43 77 36 93 44 0 10.7 5.8 147 5.2 105 2 9.3 5.5 147 5.6 104 1 10.6 8.1 147 6.2 103 2 10.1 5.6 148 6.5 107 1 10.4 5.4 145 5.6 104 3 11.1 6.4 150 6.5 108 1 9.9 6.6 149 5.9 106 3 9.7 7.1 158 7.2 113 2 10.8 6.2 149 6.1 104 0 10.3 5.3 149 6.5 108 MEAN S.D. N 108 55.4 10 77 118.8 10 2 1.1 10 10.3 .55 10 6.2 .89 10 149 3.5 10 6.1 .58 10 106 3.0 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92267 C92274 C92286 C92287 C92288 C92303 C92315 C92317 C92320 C92324 103 36 144 64 80 42 158 37 185 55 101 34 91 32 71 34 108 56 61 27 1 10.1 6.6 158 6.6 114 4 11.0 7.0 149 6.4 105 1 10.4 5.7 150 6.4 106 1 10.5 6.9 156 7.0 110 3 10.6 7.2 149 6.8 108 2 9.9 6.2 154 6.2 110 1 10.4 6.0 147 6.1 105 0 10.9 6.3 148 6.9 106 0 10.8 6.0 144 5.5 100 1 10.6 5.8 149 5.7 106 MEAN S.D. N 110 40.0 10 42 12.3 10 1 1.3 10 10.5 .34 10 6.4 .53 10 150 4.3 10 6.4 .50 10 107 3.8 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4137 Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92341 C92356 C92357 C92365 C92372 C92376 C92388 C92389 C92390 C92395 119 13 .8 8.2 6.1 2.1 .7 157 92 11 .8 9.4 6.4 3.0 .2 152 143 10 .7 8.0 5.9 2.1 .7 214 119 11 .8 8.1 5.9 2.2 .2 160 113 12 .8 8.7 6.0 2.7 .2 128 100 10 .6 8.5 5.9 2.6 .2 156 131 11 .8 8.4 6.4 2.0 .2 104 120 11 .8 8.0 5.6 2.4 .1 114 105 12 .7 8.0 5.2 2.8 .2 98 86 13 .8 8.2 5.8 2.4 .2 142 MEAN S.D. N 113 11 .8 8.4 5.9 2.4 17.5 1.1 .07 .44 .36 .34 10 10 10 10 10 10 .3 142 .22 34.0 10 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92406 C92407 C92411 C92418 C92422 C92424 C92433 C92438 C92441 C92449 117 17 .9 9.0 5.8 3.2 .2 128 95 14 .9 8.9 6.1 2.8 .2 112 98 13 .8 8.9 5.4 3.5 .2 128 111 10 .8 8.3 6.1 2.2 .2 105 115 13 .7 8.2 5.9 2.3 .2 129 122 15 .8 7.7 5.4 2.3 .1 105 108 15 .9 9.0 6.4 2.6 .2 146 103 13 .8 8.6 5.8 2.8 .3 133 110 10 .7 7.9 5.1 2.8 .2 171 99 17 .8 8.6 6.1 2.5 .1 108 MEAN S.D. N 108 14 .8 8.5 5.8 2.7 8.9 2.5 .07 .47 .40 .41 10 10 10 10 10 10 .2 126 .06 20.8 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4138 Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92341 C92356 C92357 C92365 C92372 C92376 C92388 C92389 C92390 C92395 119 83 95 50 77 36 80 53 85 36 86 30 70 46 119 77 168 101 97 29 1 10.4 5.8 157 5.1 104 3 11.6 5.6 143 5.2 100 1 11.2 5.9 150 5.5 97 2 10.9 4.4 146 5.4 104 2 11.7 6.0 144 5.4 102 2 11.5 5.9 143 5.3 101 1 10.9 5.2 148 5.8 105 1 10.6 4.0 148 5.9 106 3 11.4 7.2 148 6.0 102 0 10.9 3.8 147 4.8 103 MEAN S.D. N 100 29.1 10 54 24.7 10 2 1.0 10 11.1 .44 10 5.4 1.05 10 147 4.1 10 5.4 .37 10 102 2.6 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92406 C92407 C92411 C92418 C92422 C92424 C92433 C92438 C92441 C92449 213 130 100 47 88 39 76 32 102 40 77 30 160 120 593 213 67 25 84 43 2 11.2 6.2 145 4.7 100 0 10.3 4.6 143 4.2 97 1 11.0 6.7 150 6.3 105 0 11.2 6.3 148 5.4 105 2 11.6 5.6 147 6.3 105 0 10.3 5.1 146 5.5 103 2 11.3 4.5 149 5.4 104 9 11.7 8.0 149 5.5 104 2 10.5 4.4 146 4.6 104 2 10.9 5.3 150 5.5 108 MEAN S.D. N 156 160.1 10 72 62.1 10 2 2.6 10 11.0 .50 10 5.7 1.14 10 147 2.3 10 5.3 .68 10 104 3.0 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4139 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 10.3 1.021 7.0 31.0 1.006 7.5 5.6 1.038 6.5 8.3 1.027 7.0 32.9 1.007 7.0 42.4 1.004 7.5 48.5 a 1.003 7.0 13.7 1.020 7.0 24.0 1.008 7.0 10.6 1.020 7.5 MEAN S.D. N 22.7 15.31 10 1.015 .0116 10 7.1 .32 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 16.2 1.015 7.0 47.8 a 1.005 7.0 23.0 1.014 7.0 12.4 1.019 7.5 20.3 1.014 7.5 5.5 1.036 7.0 17.4 1.019 7.0 27.1 1.012 7.0 9.5 1.023 7.5 39.4 1.010 7.0 MEAN S.D. N 21.9 13.22 10 1.017 .0085 10 7.2 .24 10 a Sample was overflowing at the time of collection. 4140 Covance 6329-212 Covance 6329-228 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92191 30 - - - - - C92193 10 - - - + - C92194 30 - - - + - C92195 30 - - - - - C92197 10 - - - - - C92199 0 - - - - - C92200 30 - - - +++ - C92201 30 - - - +++ - C92202 10 - - - - - C92203 30 - + - - - Group: 9 Dose Level: 1 Dosage Unit: ppm C92261 10 - - - - - C92262 10 - - - - - C92263 10 - - - - - C92265 30 - - - + - C92266 10 - - - - - C92267 30 - + - - - C92268 30 - - - - - C92269 10 - - - - - C92270 30 - - - - - C92271 10 - - - - - Covance 6329-212 Covance 6329-228 3M T-6316.1 4141 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92191 0 0 1 1 0 4D BJ PQ C92193 0 0 1 2 0 0 AJ Q C92194 0 0 1 1 0 4D BJ PQ C92195 0 0 0 2 0 4D BJ PQ C92197 0 0 0 1 0 0 AJ Q C92199 0 1 1 2 0 0 AJ P C92200 0 0 1 1 0 0 AJ Q C92201 1 0 1 2 0 4D BJ PQ C92202 1 1 1 1 0 0 AJ Q C92203 0 0 1 2 0 4D BJ PQ Group: 9 Dose Level: 1 Dosage Unit: ppm C92261 0 0 1 2 0 4D BJ PQ C92262 0 0 1 2 0 0 AJ Q C92263 0 0 0 2 0 0 BJ Q C92265 0 0 1 2 0 4D BJ PQ C92266 0 0 1 2 0 4D BJ PQ C92267 0 0 1 2 0 4D BJ PQ C92268 0 0 1 2 0 4D BJ PQ C92269 0 0 1 2 0 0 BJ Q C92270 0 0 0 2 0 4D BK Q C92271 0 0 1 1 0 0 AJ Q Covance 6329-212 Covance 6329-228 3M T-6316.1 4142 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 9.0 1.013 7.0 50.5 a 1.001 6.5 4.2 1.024 6.5 5.1 1.020 7.0 10.3 1.016 6.5 5.0 1.024 6.5 16.2 1.015 7.0 11.9 1.011 7.0 5.3 1.028 6.5 7.3 1.014 7.0 MEAN S.D. N 12.5 13.88 10 1.017 .0078 10 6.8 .26 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 22.3 1.007 7.0 40.8 1.001 6.5 4.6 1.026 6.5 49.8 a 1.002 7.0 5.6 1.006 7.0 2.0 1.034 6.5 17.8 1.009 7.0 18.5 1.010 6.5 50.7 a 1.002 6.5 7.5 1.020 6.5 MEAN S.D. N 22.0 18.74 10 1.012 .0112 10 6.7 .26 10 a Sample was overflowing at the time of collection. 4143 Covance 6329-212 Covance 6329-228 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92331 10 - - - - - C92333 0 - - - - - C92334 10 - - - - - C92336 10 - - - - - C92337 10 - - - - - C92338 10 - - - - - C92339 0 - - - - - C92340 0 - - - - - C92341 100 - - - - - C92342 30 - - - + - Group: 9 Dose Level: 1 Dosage Unit: ppm C92401 0 - - - - - C92403 0 - - - - - C92404 30 - - - +++ - C92405 0 - - - - - C92406 0 - - - - - C92407 30 - - - - - C92408 0 - - - - - C92409 0 - - - - - C92410 0 - - - - - C92411 10 - - - - - Covance 6329-212 Covance 6329-228 3M T-6316.1 4144 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92331 0 0 0 2 0 4D BJ P C92333 0 0 0 1 0 0 AJ - C92334 0 0 1 2 0 4D BJ P C92336 0 0 1 3 0 3B4D BJ P C92337 0 0 1 1 0 1D BJ P C92338 0 0 1 2 0 4D BJ P C92339 0 0 1 1 0 4D BJ - C92340 0 0 1 1 0 4D BJ P C92341 0 0 1 1 0 1D BJ P C92342 0 0 0 2 0 4D BJ - Group: 9 Dose Level: 1 Dosage Unit: ppm C92401 0 0 1 2 0 0 AJ P C92403 0 0 0 1 0 0 AJ - C92404 1 1 1 2 0 3D BJ P C92405 0 0 0 1 0 0 AJ - C92406 0 0 1 1 0 0 AJ - C92407 0 0 1 2 0 4D BJ P C92408 0 0 1 1 0 0 AJ - C92409 0 1 1 2 0 1B2D BJ P C92410 0 0 0 1 0 0 AJ - C92411 0 0 1 2 0 0 BJ PS Covance 6329-212 Covance 6329-228 3M T-6316.1 4145 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 10.0 1.020 7.0 8.3 1.024 7.5 8.0 1.032 6.5 5.5 1.033 7.0 44.5 1.006 7.0 17.5 1.014 7.5 9.5 1.020 6.5 5.8 1.027 6.5 7.2 1.026 6.5 22.0 1.020 7.0 MEAN S.D. N 13.8 12.00 10 1.022 .0081 10 6.9 .39 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 9.0 1.027 7.5 7.8 1.031 6.5 12.3 1.019 7.0 48.0 a 1.008 7.0 8.0 1.032 7.0 1.6 1.053 7.0 11.8 1.031 7.0 23.0 1.017 7.0 9.3 1.023 7.5 23.2 1.015 7.0 MEAN S.D. N 15.4 13.27 10 1.026 .0125 10 7.0 .28 10 a Sample was overflowing at the time of collection. 4146 Covance 6329-212 Covance 6329-228 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92191 10 - - - - - C92193 100 - ++ - - - C92194 100 - - - - - C92195 10 - - - - - C92197 0 - - - - - C92199 10 - - - - - C92200 100 - - - - - C92201 30 - - - - - C92202 100 - - - + - C92203 100 - + - - - Group: 9 Dose Level: 1 Dosage Unit: ppm C92261 30 - + - - - C92262 30 - + - - - C92263 30 - - - - - C92265 100 - - - - - C92266 10 - - - - - C92267 100 - + - - - C92268 100 - - - - - C92269 10 - + - - - C92270 10 - + - - - C92271 30 - - - + - Covance 6329-212 Covance 6329-228 3M T-6316.1 4147 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92191 0 0 1 1 0 4D BJ PQ C92193 0 0 0 1 0 3D BJ Q C92194 0 0 0 1 0 1B BJ - C92195 0 0 1 3 0 4D BK PQ C92197 0 0 1 1 0 0 AJ Q C92199 0 0 1 1 0 3D BJ Q C92200 0 0 1 2 0 1D BJ PQ C92201 0 0 1 2 0 0 BJ PQ C92202 0 1 1 2 0 1B1D BJ Q C92203 0 0 1 3 0 4D BJ Q Group: 9 Dose Level: 1 Dosage Unit: ppm C92261 0 0 0 2 0 4D BJ Q C92262 0 0 1 1 0 3D BJ PQ C92263 0 1 0 2 0 1D BJ Q C92265 0 0 1 2 0 4D AJ PQ C92266 0 0 1 1 0 4D BJ Q C92267 1 0 1 1 0 3D BJ PQ C92268 0 0 1 1 0 4D BJ PQ C92269 0 0 1 2 0 4D BJ PQ C92270 0 0 0 1 0 3D BJ PQ C92271 1 0 0 1 0 4D BJ PQ Covance 6329-212 Covance 6329-228 3M T-6316.1 4148 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 7.0 1.020 6.5 2.6 1.027 6.5 2.3 1.040 7.0 47.5 a 1.003 6.5 8.3 1.016 6.5 2.6 1.041 6.5 27.0 1.011 6.5 6.8 1.013 7.0 47.0 a 1.006 6.5 20.0 1.010 6.5 MEAN S.D. N 17.1 17.79 10 1.019 .0134 10 6.6 .21 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 13.0 1.020 6.5 14.2 1.019 6.5 41.8 1.006 6.5 9.8 1.014 6.5 .5 1.047 6.5 46.0 1.005 6.5 43.5 1.006 6.5 5.0 1.026 7.0 10.0 1.020 6.5 15.8 1.012 6.5 MEAN S.D. N 20.0 17.04 10 1.018 .0126 10 6.6 .16 10 a Sample was overflowing at the time of collection. 4149 Covance 6329-212 Covance 6329-228 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92331 100 - - - - - C92333 10 - - - - - C92334 10 - - - - - C92336 0 - - - - - C92337 0 - - - - - C92338 10 - - - - - C92339 0 - - - - - C92340 0 - - - - - C92341 100 - - - - - C92342 30 - - - - - Group: 9 Dose Level: 1 Dosage Unit: ppm C92401 0 - - - - - C92403 10 - - - - - C92404 0 - - - + - C92405 10 - - - - - C92406 10 - - - - - C92407 0 - - - - - C92408 0 - - - - - C92409 10 - - - - - C92410 0 - - - - - C92411 0 - - - - - Covance 6329-212 Covance 6329-228 3M T-6316.1 4150 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92331 0 0 1 1 0 0 BJ P C92333 0 1 1 1 0 0 BJ P C92334 0 0 1 2 0 4D BJ PQ C92336 0 0 1 1 0 0 AJ - C92337 0 0 1 1 0 1D BJ P C92338 0 1 1 1 0 3D BJ P C92339 0 0 1 1 0 0 BJ - C92340 0 0 1 1 0 0 BJ - C92341 0 0 1 1 0 0 AJ - C92342 0 0 1 2 0 2B BJ - Group: 9 Dose Level: 1 Dosage Unit: ppm C92401 0 0 1 1 0 0 BJ - C92403 0 0 1 1 0 0 BJ - C92404 0 0 0 3 0 2B BJ - C92405 0 0 1 1 0 0 BJ P C92406 QS - - - - - - BJ - C92407 0 0 1 2 0 0 AJ - C92408 0 0 1 1 0 0 AJ - C92409 0 0 1 1 0 4D BJ P C92410 0 1 1 1 0 0 BJ - C92411 0 0 1 2 0 0 BJ - Covance 6329-212 Covance 6329-228 3M T-6316.1 4151 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92195 C92199 C92205 C92211 C92223 C92230 C92238 C92246 C92247 C92253 15.2 1.014 7.5 9.8 1.021 7.0 18.0 1.025 7.0 49.5 a 1.004 7.0 7.0 1.034 7.0 10.3 1.030 7.0 14.2 1.024 6.5 6.3 1.041 7.5 9.2 1.037 7.0 7.3 1.027 6.5 MEAN S.D. N 14.7 12.83 10 1.026 .0110 10 7.0 .33 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92267 C92274 C92286 C92287 C92288 C92303 C92315 C92317 C92320 C92324 3.3 1.055 7.0 12.3 1.024 7.0 7.4 1.038 6.5 12.5 1.020 7.0 6.7 1.032 7.0 12.5 1.023 7.0 3.6 1.037 7.0 5.0 1.038 7.0 21.5 1.017 6.5 20.0 1.016 7.0 MEAN S.D. N 10.5 6.47 10 1.030 .0123 10 6.9 .21 10 a Sample was overflowing at the time of collection. 4152 Covance 6329-212 Covance 6329-228 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92195 30 - - - - - C92199 100 - - - - - C92205 300 - - - - - C92211 0 - - - - - C92223 >2000 - - - - - C92230 100 - - - - - C92238 300 - - - - - C92246 100 - - - + - C92247 >2000 - - - - - C92253 100 - - - - - Group: 9 Dose Level: 1 Dosage Unit: ppm C92267 300 - + - - - C92274 100 - + - +++ - C92286 300 - - - - - C92287 100 - - - - - C92288 300 - - - - - C92303 30 - + - - - C92315 300 - - - - - C92317 300 - + - + - C92320 100 - - - - - C92324 300 - - - - - Covance 6329-212 Covance 6329-228 3M T-6316.1 4153 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92195 0 0 0 2 0 4D BJ Q C92199 0 0 0 1 0 2D BJ P C92205 0 0 0 2 0 3D BJ PQ C92211 0 0 1 1 0 0 AJ Q C92223 0 1 1 2 1G1H 4D BJ PQ C92230 0 1 1 1 0 4D BJ Q C92238 0 0 0 1 0 0 BJ Q C92246 3 0 1 2 0 2B4D BJ P C92247 0 1 1 1 1H 0 BJ PQ C92253 0 0 0 1 0 3D BJ Q Group: 9 Dose Level: 1 Dosage Unit: ppm C92267 0 0 1 1 1G 4D CJ PQ C92274 3 1 1 1 0 4D BJ PQ C92286 0 1 1 1 1G1H 0 BJ P C92287 0 0 0 1 0 1D BJ Q C92288 1 1 1 2 0 4D BJ PQ C92303 0 1 0 1 0 4D BJ Q C92315 0 0 1 1 0 4D BJ PQ C92317 4 0 0 2 0 4D BJ Q C92320 0 0 0 1 0 0 BJ Q C92324 0 1 1 1 1G 0 BJ PQ Covance 6329-212 Covance 6329-228 3M T-6316.1 4154 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92341 C92356 C92357 C92365 C92372 C92376 C92388 C92389 C92390 C92395 49.0 a 1.004 6.5 4.0 1.023 6.5 16.2 1.021 6.5 8.0 1.024 6.5 25.4 1.012 6.5 20.0 1.009 7.0 49.0 a 1.004 6.5 3.0 1.032 6.0 49.2 a 1.002 6.5 48.5 a 1.005 6.5 MEAN S.D. N 27.2 19.88 10 1.014 .0106 10 6.5 .24 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92406 C92407 C92411 C92418 C92422 C92424 C92433 C92438 C92441 C92449 2.6 1.039 6.5 8.5 1.018 6.0 13.4 1.017 6.5 18.7 1.011 6.5 4.6 1.025 7.0 38.9 1.006 7.0 12.0 1.016 6.5 13.3 1.013 6.5 8.3 1.026 6.5 10.0 1.016 6.0 MEAN S.D. N 13.0 10.19 10 1.019 .0093 10 6.5 .33 10 a Sample was overflowing at the time of collection. 4155 Covance 6329-212 Covance 6329-228 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92341 100 - - - - - C92356 100 - - - - - C92357 300 - - - - - C92365 10 - - - - - C92372 10 - - - - - C92376 10 - - - - - C92388 0 - - - - - C92389 30 - - - - - C92390 0 - - - - - C92395 0 - - - - - Group: 9 Dose Level: 1 Dosage Unit: ppm C92406 100 - - - - - C92407 100 - - - - - C92411 10 - - - + - C92418 10 - - - - - C92422 10 - - - - - C92424 10 - - - - - C92433 30 - - - - - C92438 300 - - - - - C92441 30 - - - - - C92449 10 - - - +++ - Covance 6329-212 Covance 6329-228 3M T-6316.1 4156 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92341 0 0 1 2 1G 0 AJ P C92356 0 1 1 2 1G 1B BJ P C92357 0 1 1 1 2G 0 BJ P C92365 0 1 1 1 0 0 BJ P C92372 0 0 0 3 0 0 BJ - C92376 0 0 0 1 0 0 AJ - C92388 0 0 1 1 0 0 AJ - C92389 0 1 1 1 0 0 BJ P C92390 0 0 0 1 0 0 AJ - C92395 0 0 1 2 0 0 AJ - Group: 9 Dose Level: 1 Dosage Unit: ppm C92406 0 4 1 2 0 1B BJ P C92407 0 1 1 2 1G 0 BJ P C92411 0 0 0 1 0 0 BJ - C92418 0 0 1 1 0 0 BJ P C92422 0 0 1 3 0 1B4D BJ P C92424 0 0 1 2 0 0 AJ - C92433 0 0 1 1 0 0 BJ P C92438 0 0 1 1 1G 0 BJ P C92441 0 0 1 1 0 0 BJ - C92449 4 1 1 1 0 0 BJ - Covance 6329-212 Covance 6329-228 3M T-6316.1 4157 Appendix 5 Individual Urine Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92191 C92193 C92194 C92195 C92197 C92199 C92200 a C92201 C92202 C92203 33 111 .34 1.14 12 29 .37 .90 42 254 .24 1.42 57 129 .47 1.07 13 42 .43 1.38 10 18 .42 .76 7 16 - - 18 73 .25 1.00 13 35 .31 .84 34 93 .36 .99 MEAN S.D. N 24 16.7 10 80 72.9 10 .35 .079 9 1.06 .227 9 Group: 9 Dose Level: 1 Dosage Unit: ppm C92261 C92262 a C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 25 74 .40 1.20 8 25 - - 24 69 .55 1.59 33 96 .41 1.19 41 74 .83 1.50 52 183 .29 1.01 30 115 .52 2.00 20 80 .54 2.17 49 124 .47 1.18 15 50 .59 1.97 MEAN S.D. N 30 14.3 10 89 43.9 10 .51 .151 9 1.53 .425 9 a Unable to calculate excretion due to inaccurate urine volume. 4158 Covance 6329-212 Covance 6329-228 3M T-6316.1 Appendix 5 Individual Urine Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92331 C92333 a C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 20 60 .18 .54 6 9 - - 40 103 .17 .43 28 103 .14 .53 33 64 .34 .66 42 95 .21 .48 29 82 .47 1.33 18 45 .21 .54 32 131 .17 .69 10 71 .07 .52 MEAN S.D. N 26 12.0 10 76 34.6 10 .22 .119 9 .64 .273 9 Group: 9 Dose Level: 1 Dosage Unit: ppm C92401 C92403 C92404 C92405 a C92406 C92407 C92408 C92409 C92410 a C92411 18 56 .40 1.25 6 11 .24 .45 27 112 .12 .52 6 15 - - 14 27 .08 .15 45 126 .09 .25 16 40 .28 .71 20 32 .37 .59 5 15 - - 18 78 .14 .58 MEAN S.D. N 18 12.0 10 51 41.3 10 .22 .126 8 .56 .333 8 a Unable to calculate excretion due to inaccurate urine volume. 4159 Covance 6329-212 Covance 6329-228 3M T-6316.1 Appendix 5 Individual Urine Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 17 84 .17 .84 24 127 .20 1.05 20 190 .16 1.52 38 186 .21 1.02 3 19 .13 .85 10 70 .18 1.22 22 92 .21 .87 15 120 .09 .70 15 146 .11 1.05 14 90 .31 1.98 MEAN S.D. N 18 9.3 10 112 52.8 10 .18 .062 10 1.11 .383 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92261 C92262 C92263 C92265 a C92266 C92267 C92268 C92269 C92270 C92271 48 142 .43 1.28 16 166 .12 1.29 8 82 .10 1.01 8 41 - - 42 138 .34 1.10 53 476 .08 .76 27 166 .32 1.96 10 72 .23 1.66 41 160 .38 1.49 5 66 .12 1.53 MEAN S.D. N 26 18.7 10 151 123.3 10 .24 .135 9 1.34 .363 9 a Unable to calculate excretion due to inaccurate urine volume. 4160 Covance 6329-212 Covance 6329-228 3M T-6316.1 Appendix 5 Individual Urine Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92331 C92333 C92334 C92336 a C92337 C92338 C92339 C92340 C92341 a C92342 16 134 .11 .94 24 124 .06 .32 47 253 .11 .58 4 62 - - 9 65 .07 .54 42 232 .11 .60 3 24 .08 .65 7 56 .05 .38 7 18 - - 3 43 .06 .86 MEAN S.D. N 16 16.3 10 101 83.6 10 .08 .025 8 .61 .212 8 Group: 9 Dose Level: 1 Dosage Unit: ppm C92401 C92403 C92404 C92405 C92406 QS C92407 C92408 C92409 C92410 C92411 13 115 .17 1.50 9 109 .13 1.55 5 24 .21 1.00 7 55 .07 .54 - - - - 1 10 .05 .46 5 21 .22 .91 26 136 .13 .68 11 109 .11 1.09 9 55 .14 .87 MEAN S.D. N 10 7.1 9 70 47.4 9 .14 .057 9 .96 .383 9 a Unable to calculate excretion due to inaccurate urine volume. 4161 Covance 6329-212 Covance 6329-228 3M T-6316.1 Appendix 5 Individual Urine Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92195 C92199 C92205 C92211 a C92223 C92230 C92238 C92246 C92247 C92253 16 88 .24 1.34 19 105 .19 1.03 26 158 .47 2.84 2 10 - - 13 183 .09 1.28 41 182 .42 1.87 8 124 .11 1.76 42 254 .26 1.60 14 200 .13 1.84 21 123 .15 .90 MEAN S.D. N 20 13.0 10 143 68.2 10 .23 .135 9 1.61 .579 9 Group: 9 Dose Level: 1 Dosage Unit: ppm C92267 C92274 C92286 C92287 C92288 C92303 C92315 C92317 C92320 C92324 64 361 .21 1.19 14 124 .17 1.53 35 211 .26 1.56 34 104 .42 1.30 17 174 .11 1.17 21 111 .26 1.39 14 207 .05 .75 42 212 .21 1.06 8 72 .17 1.55 8 70 .16 1.40 MEAN S.D. N 26 17.9 10 165 88.6 10 .20 .100 10 1.29 .256 10 a Unable to calculate excretion due to inaccurate urine volume. 4162 Covance 6329-212 Covance 6329-228 3M T-6316.1 Appendix 5 Individual Urine Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 8 Dose Level: 0 Dosage Unit: ppm C92341 a C92356 C92357 C92365 C92372 C92376 C92388 a C92389 C92390 a C92395 a 11 51 - - 15 77 .06 .31 26 115 .42 1.86 30 133 .24 1.06 20 46 .51 1.17 11 39 .22 .78 2 17 - - 10 138 .03 .41 3 12 - - 4 17 - - MEAN S.D. N 13 9.6 10 64 48.6 10 .25 .191 6 .93 .569 6 Group: 9 Dose Level: 1 Dosage Unit: ppm C92406 C92407 C92411 C92418 C92422 C92424 C92433 C92438 C92441 C92449 51 298 .13 .77 10 75 .08 .64 25 86 .34 1.15 8 59 .15 1.10 34 295 .16 1.36 7 20 .27 .78 14 69 .17 .83 8 53 .11 .70 31 164 .26 1.36 10 59 .10 .59 MEAN S.D. N 20 14.9 10 118 101.1 10 .18 .085 10 .93 .290 10 a Unable to calculate excretion due to inaccurate urine volume. 4163 Covance 6329-212 Covance 6329-228 3M T-6316.1 Covance 6329-212 Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Author: Peter J. Thomford, PhD Study Completion Date: December 13, 2001 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-228 Sponsor Study Identification: 3M T-6316.1 Volume XI of XII Page 4164 of 4873 Covance 6329-212 Covance 6329-228 3M T-6316.1 VOLUME XI OF XII CONTENTS Page APPENDIX 6............................................................................................................ 4166 Individual Anatomic Pathology Data ..................................................................... 4167 4165 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 Covance 6329-228 3M T-6316.1 4166 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92191 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 882.5 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 14:55 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK KIDNEY (KD) : 64; 135 X 100MM; VERIFIED AT NECROPSY: -NEPHROPATHY, CHRONIC PROGRESSIVE,- OBSERVED MINIMAL, MULTI-FOCAL >EYES; PALE; EYES; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -LARGE; ALL LOBES: MARGINS APPEAR -VACUOLATION, HEPATOCELLULAR,-SLIGHT, ROUNDED MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, MULTI- FOCAL -HYPERPLASIA, FOLLICULAR CELL,-SLIGHT, FOCAL SKIN (SK) : >NOTE:>COLLECTED FROM RIGHT INGUINAL REGION DUE TO MM MASS HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 4167 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 2 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92191 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 882.5 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 14:55 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ COLON (CO) : -PARASITISM,-PRESENT DEATH COMMENT (DC) : -MAMMARY FIBROADENOMA,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); LEFT INGUINAL REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 130 X 100 X 65 MM, DARK RED, RED, TAN, GRAY, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 4168 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 3 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92191 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 882.5 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 14:55 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4169 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92192 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 313.3 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:55 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 10.9046 3.4806 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : UNVERIFIED AT NECROPSY -NO MACROSCOPIC LESIONS -------------------------------------------- 4170 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 5 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92192 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 313.3 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:55 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4171 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 6 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92193 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 645.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:42 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- KIDNEY (KD) : LEFT; VERIFIED AT NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT BRAIN (BR) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -HYPERTROPHY,-MODERATE, FOCAL -VACUOLATION,-SLIGHT, FOCAL PITUITARY (PI) : PITUITARY (PI) : -RAISED AREA(S); ANTERIOR ASPECT: -HEMORRHAGE,-MODERATE, MULTI-FOCAL SINGLE DARK RED RAISED AREA, 2 MM IN -B-ADENOMA,-NEOPLASM PRESENT DIAMETER PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4172 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 7 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92193 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 645.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:42 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: -EROSION,-MINIMAL, FOCAL SINGLE DARK RED, DRY CRUSTED AREA, 5 -FIBROSIS,-MODERATELY SEVERE, FOCAL MM IN DIAMETER; COLLECTED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4173 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92193 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 645.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:42 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4174 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 9 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92194 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/11/00 STUDY DAY OF DEATH: 706 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 824.1 GRAMS DATE AND TIME OF NECROPSY: 03/11/00 14:54 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; PALE; EYES; VERIFIED AT LUNG (LU) : LUNG (LU) : NECROPSY: NOT APPLICABLE -DIFFUSELY RED; ALL LOBES -INFILTRATE, MACROPHAGE, ALVEOLAR,- >BEHAVIOR; HYPOACTIVE; VERIFIED AT SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE -MINERALIZATION, VASCULAR,-MINIMAL, >EXCRETION; NO FECES; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : KIDNEY (KD) : >EYES; DILATED PUPIL; EYES; VERIFIED AT -DIFFUSELY LIGHT; BILATERAL: TAN -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE MODERATE, MULTI-FOCAL >RESPIRATION; AUDIBLE; VERIFIED AT -PYELONEPHRITIS,-SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >RESPIRATION; LABORED; VERIFIED AT -RED FOCUS(I)/AREA(S); ALL LOBES, -CYST,-PRESENT NECROPSY: NOT APPLICABLE DIFFUSE: MULTIPLE DARK RED, PINPOINT -DEGENERATION, CYSTIC,-MODERATE, MULTI- >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; FOCI; COLLECTED ON ROUTINE SECTIONS FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE -DIFFUSELY LIGHT; ALL LOBES: TAN -FOCUS, ALTERED HEPATOCELLULAR, -CYST(S); ALONG MARGIN OF LEFT LATERAL EOSINOPHILIC,-MODERATE, MULTI-FOCAL LOBE: TWO CLEAR FLUID FILLED CYSTS, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL ONE IS 3 MM IN DIAMETER, THE OTHER IS -HYPERPLASIA, BILE DUCT,-MODERATE, 5 MM IN DIAMETER; BOTH ARE COLLECTED MULTI-FOCAL ON THE ROUTINE SECTION -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- -ROUGH SURFACE, DIFFUSE; ALL LOBES FOCAL -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, FOCAL 4175 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 10 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92194 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/11/00 STUDY DAY OF DEATH: 706 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 824.1 GRAMS DATE AND TIME OF NECROPSY: 03/11/00 14:54 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PARATHYROID (PT) : -HYPERPLASIA,-MODERATE, DIFFUSE HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL -THROMBUS, ATRIAL,-MODERATELY SEVERE, FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MINIMAL, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EROSION,-MODERATE, FOCAL MULTIPLE DARK BROWN AREAS, UP TO 3 X 2 MM URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,- MODERATE, DIFFUSE PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,- URETHRA: TAN MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 18 X 10 X 3 -INFLAMMATION, ACUTE,-SLIGHT, MULTI- MM FOCAL DEATH COMMENT (DC) : -HEPATOCELLULAR NECROSIS,-PRESENT 4176 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 11 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92194 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/11/00 STUDY DAY OF DEATH: 706 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 824.1 GRAMS DATE AND TIME OF NECROPSY: 03/11/00 14:54 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -DIFFUSELY RED -DILATATION, SINUSOIDAL,-SLIGHT, MULTI- -LARGE; UP TO 6 X 5 X 5 MM; APPEARS FOCAL CYSTIC (CAP 22) -HEMORRHAGE,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL OBESE -FLUID-THORACIC CAVITY; CONTAINS A LARGE AMOUNT OF CLEAR FLUID -------------------------------------------- 4177 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 12 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92194 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/11/00 STUDY DAY OF DEATH: 706 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 824.1 GRAMS DATE AND TIME OF NECROPSY: 03/11/00 14:54 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4178 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 13 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92195 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 639.6 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:39 PROSECTOR: MONTI THOMAS MAGUIRE RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0220 .0034 % .0105 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0313 .0049 % .0150 WEIGHT TAKEN BRAIN (BR) 2.0881 .3265 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.9642 .3071 % .9407 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.0438 .3195 % .9788 WEIGHT TAKEN LIVER (LI) 15.3839 2.4052 % 7.3674 WEIGHT TAKEN LUNG (LU) 1.6924 .2646 % .8105 WEIGHT TAKEN SPLEEN (SP) .7077 .1106 % .3389 WEIGHT TAKEN LEFT TESTIS (TE0) 1.6067 .2512 % .7695 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.6857 .2636 % .8073 WEIGHT TAKEN LF THYROID/PARA (TT0) .0261 .0041 % .0125 WEIGHT TAKEN RT THYROID/PARA (TT1) .0194 .0030 % .0093 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; PITUITARY (PI) : VERIFIED AT NECROPSY: OBSERVED -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE -BONE MARROW SMEAR TAKEN -------------------------------------------- 4179 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92195 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 639.6 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:39 PROSECTOR: MONTI THOMAS MAGUIRE RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4180 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 15 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92196 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 299.0 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:47 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 9.9178 3.3170 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : UNVERIFIED AT NECROPSY -NO MACROSCOPIC LESIONS -------------------------------------------- 4181 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 16 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92196 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 299.0 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:47 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4182 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 17 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92197 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 521.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:21 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: NOT OBSERVED -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT >SKIN & PELAGE; SORE/SCAB; DORSAL; -GRANULAR MATERIAL; RIGHT, WITHIN -HEMORRHAGE,-SLIGHT, MULTI-FOCAL VERIFIED AT NECROPSY: OBSERVED PELVIS: MULTIPLE TAN AND FIRM -MINERALIZATION, PELVIC,-MINIMAL, FOCAL >APPEARANCE; THIN; VERIFIED AT GRANULES, UP TO 1 MM IN DIAMETER -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: OBSERVED MODERATE, MULTI-FOCAL >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -MASS(ES); RIGHT MEDIAN LOBE: SINGLE -DEGENERATION, CYSTIC,-MINIMAL, FOCAL MASS, 27 X 28 X 15 MM, BROWN, TAN AND -HEMORRHAGE,-SLIGHT, FOCAL FIRM; SAME ON CUT SURFACE; COLLECTED; -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- REGIONAL LN (HEPATIC) NORMAL (CAP 23) FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL TESTIS (TE) : TESTIS (TE) : -SMALL; RIGHT: 24 X 15 X 7 MM (LEFT IS -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE 25 X 16 X 11 MM); RIGHT IS SOFT >NOTE:>RIGHT TESTIS SKIN (SK) : SKIN (SK) : -RAISED AREA(S); MID DORSAL LUMBAR -HEMORRHAGE,-SLIGHT, MULTI-FOCAL REGION: SINGLE TAN AREA, 3 X 2 MM -INFLAMMATION, ACUTE,-MINIMAL, MULTI- FOCAL 4183 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 18 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92197 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 521.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:21 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT BRAIN (BR) : -MINERALIZATION,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, OTHER (LN) : >UNREMARKABLE >NOTE:>HEPATIC LYMPH NODE JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; LEFT TARSAL JOINT: UP TO 20 MM -EDEMA,-MODERATE, MULTI-FOCAL IN DIAMETER; COLLECTED -EXOSTOSES,-MODERATE, FOCAL -INFLAMMATION, ACUTE, SYNOVIAL,-SLIGHT, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4184 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 19 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92197 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 521.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:21 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4185 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 20 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92198 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/19/98 STUDY DAY OF DEATH: 75 STUDY WEEK OF DEATH: 11 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/19/98 13:54 PROSECTOR: TROY ELLENBECKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT -PYELONEPHRITIS,-MODERATE, MULTI-FOCAL URINARY BLADDER (UB) : URINARY BLADDER (UB) : -CALCULUS(I); WITHIN LUMEN: MULTIPLE -HYPERPLASIA, TRANSITIONAL CELL,- WHITE, TAN AND HARD CALCULI, UP TO 13 SEVERE, DIFFUSE X 10 X 8 MM -INFLAMMATION, CHRONIC-ACTIVE,- -THICKENED WALL; DIFFUSE MODERATE, MULTI-FOCAL -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL DEATH COMMENT (DC) : -CYSTITIS,-PRESENT URETER (UE) : URETER (UE) : -LARGE; BILATERAL: UP TO 2 MM IN -DILATATION,-MODERATE, DIFFUSE DIAMETER (CAP 18) GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST BROWN MATERIAL -URINARY BLADDER-LARGE LUMEN; 50 X 30 X 20 MM; CONTAINS A BROWN SEMIFLUID -AUTOLYSIS-SEVERE; ENTIRE GI TRACT 4186 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 21 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92198 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/19/98 STUDY DAY OF DEATH: 75 STUDY WEEK OF DEATH: 11 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/19/98 13:54 PROSECTOR: TROY ELLENBECKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4187 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 22 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92199 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 635.6 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 11:03 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0297 .0047 % .0125 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0343 .0054 % .0145 WEIGHT TAKEN BRAIN (BR) 2.3690 .3727 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.0154 .3171 % .8507 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.9204 .3021 % .8106 WEIGHT TAKEN LIVER (LI) 16.0651 2.5275 % 6.7814 WEIGHT TAKEN LUNG (LU) 2.1731 .3419 % .9173 WEIGHT TAKEN SPLEEN (SP) .8156 .1283 % .3443 WEIGHT TAKEN LEFT TESTIS (TE0) 1.7522 .2757 % .7396 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.6697 .2627 % .7048 WEIGHT TAKEN LF THYROID/PARA (TT0) .0238 .0037 % .0100 WEIGHT TAKEN RT THYROID/PARA (TT1) .0341 .0054 % .0144 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL STOMACH, GL (ST) : -EDEMA,-SLIGHT, FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE -BONE MARROW SMEAR TAKEN -------------------------------------------- 4188 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 23 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92199 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 635.6 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 11:03 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4189 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 24 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92200 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 534 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 673.4 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 9:55 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); TAIL-PROXIMAL; LUNG (LU) : WEEK 74; VERIFIED AT NECROPSY: -MINERALIZATION, VASCULAR,-MINIMAL, OBSERVED MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -CYST,-SLIGHT, FOCAL >EXCRETION; FEW FECES; VERIFIED AT -MINERALIZATION, PELVIC,-SLIGHT, FOCAL NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EYES; RED DISCHARGE; EYES; VERIFIED AT MINIMAL, MULTI-FOCAL NECROPSY: OBSERVED LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 5 X 5 X 7 MM, -HEMORRHAGE,-MINIMAL, MULTI-FOCAL FIRM, RED, DARK RED, TAN AND LOBULATED -B-ADENOMA,-NEOPLASM PRESENT 4190 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 25 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92200 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 534 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 673.4 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 9:55 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ RECTUM (RE) : >TISSUE MISSING PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT TAIL (TI) : TAIL (TI) : -RAISED AREA(S); NEAR BASE OF TAIL: -B-FIBROMA,-NEOPLASM PRESENT SINGLE TAN FIRM AREA, 4 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -------------------------------------------- 4191 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 26 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92200 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 534 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 673.4 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 9:55 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4192 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92201 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 662 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 682.3 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 12:33 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; LIMITED USE; LIMBS-HIND; KIDNEY (KD) : VERIFIED AT NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EXCRETION; FEW FECES; VERIFIED AT MODERATE, MULTI-FOCAL NECROPSY: NOT APPLICABLE PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 9 X 8 MM, RED, DARK -HEMORRHAGE,-MINIMAL, MULTI-FOCAL RED AND SOFT -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 4193 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 28 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92201 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 662 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 682.3 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 12:33 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4194 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 29 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92202 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/25/99 STUDY DAY OF DEATH: 599 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/25/99 7:24 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >APPEARANCE; THIN; VERIFIED AT MODERATELY SEVERE, DIFFUSE NECROPSY: NOT OBSERVED BRAIN (BR) : BRAIN (BR) : >APPEARANCE; MALOCCLUSION; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 MM IN DIAMETER, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT ILEUM (IL) : >AUTOLYTIC DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); BILATERAL PECTORAL REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL MULTIPLE TAN AND CREAMY SEMIFLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER (CAP 19) 4195 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 30 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92202 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/25/99 STUDY DAY OF DEATH: 599 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/25/99 7:24 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL UPPER INCISORS -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4196 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 31 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92203 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 554.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:28 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; KIDNEY (KD) : KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -ROUGH SURFACE, DIFFUSE; BILATERAL -CYST,-MODERATE, MULTI-FOCAL >APPEARANCE; THIN; VERIFIED AT -DIFFUSELY LIGHT; BILATERAL: TAN -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT OBSERVED SEVERE, DIFFUSE LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -DEGENERATION, CYSTIC,-MODERATE, MULTI- MULTIPLE DARK RED AREAS, UP TO 4 X 3 FOCAL MM; COLLECTED ON ROUTINE SECTIONS -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATELY SEVERE, MULTI- FOCAL PANCREAS (PA) : PANCREAS (PA) : -LARGE VESSELS; UP TO 2 MM IN DIAMETER -ATROPHY, ACINAR,-MODERATE, FOCAL (CAP 24) -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -HYPERTROPHY, VASCULAR,-MODERATELY SEVERE, MULTI-FOCAL -INFLAMMATION, VASCULAR,-MINIMAL, MULTI- FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL PARATHYROID (PT) : PARATHYROID (PT) : -LARGE; BILATERAL: EACH IS UP TO 2 MM -HYPERPLASIA,-MODERATELY SEVERE, IN DIAMETER DIFFUSE TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT, MULTI- FOCAL 4197 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 32 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92203 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 554.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:28 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TESTIS (TE) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -VACUOLATION,-MODERATE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 7 MM IN DIAMETER -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -RED FOCUS(I)/AREA(S); DIFFUSE: -B-ADENOMA,-NEOPLASM PRESENT MULTIPLE DARK RED AREAS, UP TO 1 MM IN DIAMETER STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; ALL REGIONS DIFFUSE -MINERALIZATION,-MODERATE, MULTI-FOCAL PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; BILATERAL: EACH IS 6 X 5 X 4 -DILATATION, SINUSOIDAL,-MODERATELY MM (CAP 22) SEVERE, MULTI-FOCAL -DIFFUSELY RED; BILATERAL: DARK RED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 4198 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 33 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92203 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 554.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:28 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, RENAL (RL) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: EACH IS 7 X 5 X 2 -DILATATION, SINUSOIDAL,-MODERATELY MM (CAP 23) SEVERE, MULTI-FOCAL MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; LEFT TARSAL: UP TO 15 MM IN -EDEMA,-MODERATELY SEVERE, MULTI-FOCAL DIAMETER; COLLECTED -EXOSTOSES,-MODERATELY SEVERE, MULTI- FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE, SYNOVIAL,-MODERATE, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4199 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 34 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92203 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 554.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:28 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), THYROID (TY), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4200 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 35 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92204 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 570 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/27/99 13:56 PROSECTOR: JOHN S. HALFORD RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -LARGE PELVIS(ES); BILATERAL -CAST, PROTEINACEOUS,-MINIMAL, MULTI- -GRANULAR MATERIAL; RIGHT, WITHIN FOCAL PELVIS: MULTIPLE TAN AND GRAY -DILATATION, PELVIC,-PRESENT GRANULES, PINPOINT AND UP TO 1 MM IN DIAMETER LIVER (LI) : LIVER (LI) : -LARGE; LEFT LATERAL LOBE -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATELY SEVERE, MULTI- FOCAL SPINAL CORD (SC) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL SPLEEN (SP) : SPLEEN (SP) : -LARGE; 71 X 19 X 12 MM -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 6 X 4 MM, DARK RED, -HEMORRHAGE,-SEVERE, FOCAL LOBULATED AND SOFT; MASS ADHERED TO -B-ADENOMA,-NEOPLASM PRESENT HYPOTHALAMUS OF BRAIN 4201 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 36 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92204 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 570 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/27/99 13:56 PROSECTOR: JOHN S. HALFORD RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, NONGL (SU) : -EDEMA,-MODERATELY SEVERE, FOCAL JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING >UNREMARKABLE URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT -------------------------------------------- 4202 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 37 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92204 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 570 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/27/99 13:56 PROSECTOR: JOHN S. HALFORD RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP) ------------------------------------------------------------------------------------------------------------------------------------ 4203 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 38 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92205 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 832.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:32 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0471 .0057 % .0196 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0298 .0036 % .0124 WEIGHT TAKEN BRAIN (BR) 2.4066 .2891 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.5383 .3049 % 1.0547 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.6192 .3147 % 1.0883 WEIGHT TAKEN LIVER (LI) 20.3350 2.4429 % 8.4497 WEIGHT TAKEN LUNG (LU) 2.3955 .2878 % .9954 WEIGHT TAKEN SPLEEN (SP) 1.0579 .1271 % .4396 WEIGHT TAKEN LEFT TESTIS (TE0) 1.8842 .2264 % .7829 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.9211 .2308 % .7983 WEIGHT TAKEN LF THYROID/PARA (TT0) .0299 .0036 % .0124 WEIGHT TAKEN RT THYROID/PARA (TT1) .0350 .0042 % .0145 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL LIVER (LI) : -HEMORRHAGE,-MINIMAL, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, MULTI- FOCAL HARDERIAN GLAND (HG) : -ONE EXAMINED,-PRESENT 4204 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 39 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92205 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 832.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:32 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ RECTUM (RE) : -PARASITISM,-PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE -BONE MARROW SMEAR TAKEN -------------------------------------------- 4205 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 40 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92205 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 832.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:32 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4206 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 41 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92206 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 527 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 531.2 GRAMS DATE AND TIME OF NECROPSY: 09/14/99 11:22 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >BEHAVIOR; HYPOACTIVE; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EXCRETION; FEW FECES; VERIFIED AT MODERATE, MULTI-FOCAL NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : -LARGE; ALL LOBES >UNREMARKABLE BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, RED, DARK -HEMORRHAGE,-SLIGHT, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; MUCOSA, NEAR JUNCTION >UNREMARKABLE OF NONGLANDULAR: TAN PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 4207 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 42 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92206 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 527 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 531.2 GRAMS DATE AND TIME OF NECROPSY: 09/14/99 11:22 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4208 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 43 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92207 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 316.2 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:15 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 11.1487 3.5258 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : UNVERIFIED AT NECROPSY -NO MACROSCOPIC LESIONS -------------------------------------------- 4209 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 44 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92207 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 316.2 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:15 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4210 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 45 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92208 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1038.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:47 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; DORSAL- KIDNEY (KD) : THORACIC-RIGHT; VERIFIED AT NECROPSY: -HYPERPLASIA, TRANSITIONAL CELL,- OBSERVED SLIGHT, DIFFUSE -MINERALIZATION, PELVIC,-SLIGHT, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATELY SEVERE, MULTI-FOCAL -PYELONEPHRITIS,-SLIGHT, MULTI-FOCAL LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- MULTIPLE DARK RED AREAS, UP TO 3 MM IN FOCAL DIAMETER; COLLECTED ON ROUTINE SECTIONS PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 9 X 9 X 20 -ATROPHY/DEGENERATION,-SEVERE, MULTI- MM FOCAL -CONTAINS FLUID; BILATERAL: CLEAR FLUID SKIN (SK) : -INFLAMMATION, ACUTE,-MINIMAL, FOCAL 4211 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 46 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92208 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1038.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:47 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : -CRUSTED AREA(S); RIGHT LATERAL THORACIC AND ABDOMINAL REGIONS: MULTIPLE DRY, RED CRUSTED AREAS, UP TO 3 X 4 MM (CAP 22) BRAIN (BR) : -MINERALIZATION,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,- SLIGHT, DIFFUSE -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL PROSTATE (PR) : -EDEMA,-SLIGHT, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL 4212 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 47 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92208 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1038.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:47 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; DRY, BROWN MATERIAL -ANIMAL OBESE -BONE MARROW SMEAR TAKEN -------------------------------------------- 4213 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 48 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92208 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1038.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:47 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4214 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 49 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92209 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 527.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:54 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; PALE; ENTIRE BODY; KIDNEY (KD) : VERIFIED AT NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >APPEARANCE; MALOCCLUSION; VERIFIED AT MODERATELY SEVERE, DIFFUSE NECROPSY: OBSERVED PANCREAS (PA) : -HYPERTROPHY, VASCULAR,-MODERATE, MULTI- FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -INFLAMMATION, CHRONIC,-SLIGHT, FOCAL TESTIS (TE) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 5 X 3 MM, RED, TAN AND -B-ADENOMA,-NEOPLASM PRESENT FIRM STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE BROWN AREAS, UP TO 2 X 1 MM PROSTATE (PR) : -INFLAMMATION, ACUTE,-MINIMAL, MULTI- FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4215 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 50 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92209 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 527.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:54 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: LOWER INCISORS -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -BONE MARROW SMEAR TAKEN -------------------------------------------- 4216 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 51 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92209 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 527.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:54 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4217 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 52 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92210 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/23/00 STUDY DAY OF DEATH: 689 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 560.7 GRAMS DATE AND TIME OF NECROPSY: 02/23/00 10:41 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >SKIN & PELAGE; YELLOW HAIR COAT; FOCAL VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >SKIN & PELAGE; RED HAIR COAT; PERINEAL THYROID (TY) : AREA; VERIFIED AT NECROPSY: OBSERVED -CYST, ULTIMOBRANCHIAL,-PRESENT >EXCRETION; NO FECES; VERIFIED AT TESTIS (TE) : TESTIS (TE) : NECROPSY: NOT APPLICABLE -SMALL; BILATERAL: EACH IS 20 X 10 X 8 -ATROPHY/DEGENERATION,-MODERATELY MM; BOTH ARE SOFT SEVERE, DIFFUSE BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, FOCAL -VACUOLATION,-MODERATE, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 8 X 7 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT 4218 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92210 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/23/00 STUDY DAY OF DEATH: 689 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 560.7 GRAMS DATE AND TIME OF NECROPSY: 02/23/00 10:41 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE >UNREMARKABLE BROWN ERODED AREAS, UP TO 2 X 1 MM STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; MUCOSA, DIFFUSE -EDEMA,-SLIGHT, FOCAL PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,- URETHRA: GREEN AND FIRM MODERATE, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 13 X 5 X 3 >UNREMARKABLE MM DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN (CAP >UNREMARKABLE 20) LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL: RED AND TAN (CAP -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 21) MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); BILATERAL, PECTORAL REGIONS: -GALACTOCELE,-MODERATE, MULTI-FOCAL MULTIPLE WHITE CREAMY SEMIFLUID FILLED CYSTS, UP TO 2 MM IN DIAMETER (CAP 19) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS 4219 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 54 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92210 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/23/00 STUDY DAY OF DEATH: 689 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 560.7 GRAMS DATE AND TIME OF NECROPSY: 02/23/00 10:41 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; PERIANAL: DRY RED MATERIAL; POSSIBLY CONSISTENT WITH STAINS (PERINEAL REGION) AS NOTED IN CLINICAL OBSERVATIONS -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DRY YELLOW MATERIAL -------------------------------------------- 4220 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 55 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92210 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/23/00 STUDY DAY OF DEATH: 689 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 560.7 GRAMS DATE AND TIME OF NECROPSY: 02/23/00 10:41 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4221 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 56 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92211 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 627.6 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 9:46 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0295 .0047 % .0121 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0271 .0043 % .0112 WEIGHT TAKEN BRAIN (BR) 2.4300 .3872 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.1992 .3504 % .9050 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.2819 .3636 % .9391 WEIGHT TAKEN LIVER (LI) 17.6271 2.8087 % 7.2540 WEIGHT TAKEN LUNG (LU) 2.1850 .3482 % .8992 WEIGHT TAKEN SPLEEN (SP) .9756 .1554 % .4015 WEIGHT TAKEN LEFT TESTIS (TE0) 1.8440 .2938 % .7588 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.7913 .2854 % .7372 WEIGHT TAKEN LF THYROID/PARA (TT0) .0192 .0031 % .0079 WEIGHT TAKEN RT THYROID/PARA (TT1) .0168 .0027 % .0069 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; DEATH COMMENT (DC) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 4222 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 57 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92211 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 627.6 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 9:46 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4223 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 58 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92212 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 372.6 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 15:14 PROSECTOR: JERALD W. JOHNSON RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: NOT OBSERVED -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EXCRETION; NO FECES; VERIFIED AT -DIFFUSELY LIGHT; BILATERAL: TAN SEVERE, DIFFUSE NECROPSY: NOT APPLICABLE -THROMBUS,-MODERATELY SEVERE, FOCAL >DISCHARGE; NASAL; RED IN COLOR; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -DIFFUSELY LIGHT; ALL LOBES: TAN >UNREMARKABLE >SKIN & PELAGE; RED HAIR COAT; PERINEAL PANCREAS (PA) : AREA; VERIFIED AT NECROPSY: OBSERVED -THROMBUS,-MODERATELY SEVERE, FOCAL >SKIN & PELAGE; PALE; ENTIRE BODY; PARATHYROID (PT) : PARATHYROID (PT) : VERIFIED AT NECROPSY: NOT APPLICABLE -LARGE; BILATERAL: EACH IS 1 MM IN -HYPERPLASIA,-MINIMAL, DIFFUSE DIAMETER TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 18 X 9 X 8 -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- MM; BOTH ARE SOFT FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MODERATE, FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT 4224 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 59 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92212 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 372.6 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 15:14 PROSECTOR: JERALD W. JOHNSON RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 MM IN DIAMETER, RED, TAN -B-ADENOMA,-NEOPLASM PRESENT AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: -EROSION,-MINIMAL, FOCAL MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM -THICKENED WALL; MUCOSA, DIFFUSE STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; MUCOSA, DIFFUSE -INFLAMMATION, ACUTE,-MINIMAL, MULTI- -EROSION/ULCERATION; MUCOSA: MULTIPLE FOCAL RED AND TAN ERODED AREAS, UP TO 5 MM IN DIAMETER DUODENUM (DU) : DUODENUM (DU) : -THICKENED WALL; DIFFUSE; COLLECTED ON >UNREMARKABLE ROUTINE SECTION PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 5 X 2 >UNREMARKABLE MM DEATH COMMENT (DC) : -PYELONEPHRITIS,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; RED, MOIST MATERIAL -DISCHARGE-NASAL; DRY, RED MATERIAL 4225 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 60 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92212 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 372.6 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 15:14 PROSECTOR: JERALD W. JOHNSON RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4226 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 61 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92213 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/99 STUDY DAY OF DEATH: 356 STUDY WEEK OF DEATH: 51 TERMINAL BODY WEIGHT: 506.4 GRAMS DATE AND TIME OF NECROPSY: 03/27/99 14:50 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; HEAD- LUNG (LU) : ENTIRE; VERIFIED AT NECROPSY: NOT -INFILTRATE, MACROPHAGE, ALVEOLAR,- OBSERVED MINIMAL, MULTI-FOCAL >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; -N-TRANSITIONAL CELL CARCINOMA,- VERIFIED AT NECROPSY: NOT APPLICABLE NEOPLASM PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: NOT APPLICABLE -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT -MASS(ES); LEFT: SINGLE MASS, 35 X 20 -MINERALIZATION, PELVIC,-MODERATE, X 10 MM, RED, DARK RED AND SOFT; SAME MULTI-FOCAL ON CUT SURFACE; COLLECTED WITH LEFT -NEPHROPATHY, CHRONIC PROGRESSIVE,- KIDNEY; REGIONAL LN (RENAL) NOT MINIMAL, MULTI-FOCAL IDENTIFIED -THROMBUS,-MODERATE, MULTI-FOCAL -I-TRANSITIONAL CELL CARCINOMA,- NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT, DIFFUSE TESTIS (TE) : TESTIS (TE) : -SMALL; RIGHT: 17 X 11 X 7 MM (FOR -ATROPHY/DEGENERATION,-MODERATELY COMPARISON, LEFT IS 20 X 14 X 10 MM) SEVERE, MULTI-FOCAL -MINERALIZATION,-SLIGHT, MULTI-FOCAL -SPERMATOCELE,-MODERATE, FOCAL EYE (EY) : -INFLAMMATION, ACUTE, CORNEAL,-MINIMAL, MULTI-FOCAL 4227 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 62 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92213 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/99 STUDY DAY OF DEATH: 356 STUDY WEEK OF DEATH: 51 TERMINAL BODY WEIGHT: 506.4 GRAMS DATE AND TIME OF NECROPSY: 03/27/99 14:50 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ EYE (EY) : -INFLAMMATION, ACUTE, CILIARY,-MINIMAL, MULTI-FOCAL -ULCERATION, CORNEAL,-MODERATE, FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE RED AND DARK RED AREAS, UP TO 2 X 1 MM PROSTATE (PR) : PROSTATE (PR) : -GELATINOUS; DIFFUSE: TAN AND CLEAR -I-TRANSITIONAL CELL CARCINOMA,- NEOPLASM PRESENT SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -MASS(ES); BILATERAL, INVOLVING ALL OF -M-TRANSITIONAL CELL CARCINOMA,- BOTH ORGANS: SINGLE MASS, 65 X 55 X 40 NEOPLASM PRESENT MM, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NOT IDENTIFIED DEATH COMMENT (DC) : -TRANSITIONAL CELL CARCINOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 4228 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 63 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92213 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/99 STUDY DAY OF DEATH: 356 STUDY WEEK OF DEATH: 51 TERMINAL BODY WEIGHT: 506.4 GRAMS DATE AND TIME OF NECROPSY: 03/27/99 14:50 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4229 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 64 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92214 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 600.8 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:59 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >DISCHARGE; NASAL; RED IN COLOR; KIDNEY (KD) : KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT >SKIN & PELAGE; YELLOW HAIR COAT; -DIFFUSELY LIGHT; BILATERAL: TAN -MINERALIZATION, TUBULAR,-SLIGHT, MULTI- VERIFIED AT NECROPSY: OBSERVED -LARGE; BILATERAL: EACH IS 27 X 17 X FOCAL >APPEARANCE; THIN; VERIFIED AT 14 MM -PYELONEPHRITIS,-MODERATE, MULTI-FOCAL NECROPSY: NOT OBSERVED -THROMBUS,-MODERATELY SEVERE, FOCAL >EXCRETION; NO FECES; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -DIFFUSELY LIGHT; ALL LOBES: LIGHT RED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >BEHAVIOR; HYPOACTIVE; VERIFIED AT >NOTE:>TRIMMING COMMENT: RIGHT MEDIAN FOCAL NECROPSY: NOT APPLICABLE LOBE OF LIVER MISSING AT TIME OF TRIMMING HEART (HT) : HEART (HT) : -LIGHT FOCUS(I)/AREA(S); EPICARDIUM OF >UNREMARKABLE BOTH VENTRICLES: MULTIPLE GRAY INDISTINCT AREAS ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -DIFFUSELY RED; BILATERAL: DARK RED -VACUOLATION,-MINIMAL, MULTI-FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: -EDEMA,-SLIGHT, FOCAL MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM STOMACH, NONGL (SU) : -ACANTHOSIS,-SLIGHT, DIFFUSE -HYPERKERATOSIS, NONGLANDULAR MUCOSA,- SLIGHT, DIFFUSE 4230 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 65 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92214 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 600.8 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:59 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ URINARY BLADDER (UB) : URINARY BLADDER (UB) : -CALCULUS(I); WITHIN LUMEN: MULTIPLE -HYPERPLASIA, TRANSITIONAL CELL,- TAN, HARD CALCULI, UP TO 7 MM IN MODERATELY SEVERE, DIFFUSE DIAMETER -INFLAMMATION, CHRONIC-ACTIVE,- -THICKENED WALL; DIFFUSE MODERATE, MULTI-FOCAL -DIFFUSELY RED; RED AND DARK RED -ULCERATION,-MODERATELY SEVERE, FOCAL -SEMIFLUID MATERIAL; WITHIN LUMEN: CONTAINS A YELLOWISH-BROWN SEMIFLUID MATERIAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; RIGHT: 15 X 6 X 2 MM (LEFT IS -INFLAMMATION, CHRONIC-ACTIVE,- 25 X 10 X 3 MM) MODERATE, MULTI-FOCAL DEATH COMMENT (DC) : -CYSTITIS,-PRESENT URETER (UE) : URETER (UE) : -LARGE; BILATERAL: EACH IS 1 MM IN -DILATATION,-MODERATE, DIFFUSE DIAMETER (CAP 19) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; DRY, YELLOW MATERIAL -URINARY BLADDER-LARGE LUMEN; 45 X 27 X 27 MM -DISCHARGE-NASAL; DRY, RED MATERIAL -------------------------------------------- 4231 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 66 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92214 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 600.8 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:59 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4232 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 67 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92215 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 527 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 555.0 GRAMS DATE AND TIME OF NECROPSY: 09/14/99 10:21 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -DILATATION, PELVIC,-PRESENT >EYES; SQUINTED; PERI-ORBITAL-LEFT; -NEPHROPATHY, CHRONIC PROGRESSIVE,- VERIFIED AT NECROPSY: NOT APPLICABLE SLIGHT, MULTI-FOCAL >SKIN & PELAGE; BROWN HAIR COAT; LIVER (LI) : VENTRAL-ABDOMINAL; VERIFIED AT -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- NECROPSY: OBSERVED FOCAL >SKIN & PELAGE; BROWN HAIR COAT; -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- VENTRAL-CERVICAL; VERIFIED AT FOCAL NECROPSY: OBSERVED HEART (HT) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -DEGENERATION/NECROSIS,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >EXCRETION; FEW FECES; VERIFIED AT PROSTATE (PR) : NECROPSY: NOT APPLICABLE -INFLAMMATION, ACUTE,-SLIGHT, FOCAL DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: LOWER INCISORS -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL AND CERVICAL REGIONS, HAIRCOAT: BROWN -------------------------------------------- 4233 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 68 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92215 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 527 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 555.0 GRAMS DATE AND TIME OF NECROPSY: 09/14/99 10:21 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4234 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 69 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92216 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 298.1 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:55 PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 11.8239 3.9664 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : UNVERIFIED AT NECROPSY -NO MACROSCOPIC LESIONS -------------------------------------------- 4235 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 70 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92216 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 298.1 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:55 PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4236 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 71 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92217 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/04/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 1135.5 GRAMS DATE AND TIME OF NECROPSY: 10/04/99 14:40 PROSECTOR: LYNN HORNER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; KIDNEY (KD) : WEEK 63; 140 X 100MM; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: OBSERVED SLIGHT, MULTI-FOCAL LIVER (LI) : LIVER (LI) : -RAISED FOCUS(I)/AREA(S); LEFT LATERAL -HEMATOPOIESIS, EXTRAMEDULLARY,- LOBE: SINGLE RAISED AREA, 15 X 14 X 7 MINIMAL, MULTI-FOCAL MM; COLLECTED ON ROUTINE SECTION -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL SPLEEN (SP) : SPLEEN (SP) : -LARGE; 65 X 19 X 6 MM -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, FOCAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 6 X 3 MM, APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; MUCOSA >UNREMARKABLE 4237 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 72 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92217 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/04/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 1135.5 GRAMS DATE AND TIME OF NECROPSY: 10/04/99 14:40 PROSECTOR: LYNN HORNER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL DEATH COMMENT (DC) : -FIBROMA,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); RIGHT ABDOMINAL REGION: 150 >TISSUE MISSING X 120 X 75 MM, RED, TAN, AND FIRM; >NOTE:>MAMMARY MASS DESCRIBED GROSSLY SAME ON CUT SURFACE; PORTIONS WAS A SUBCUTANEOUS FIBROMA COLLECTED; OVERLYING SKIN HAS A DARK RED, DRY, CRUSTED AREA, 50 X 35 MM; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER NOT FOUND AT NECROPSY DUE TO LARGE MAMMARY GLAND MASS -------------------------------------------- 4238 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 73 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92217 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/04/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 1135.5 GRAMS DATE AND TIME OF NECROPSY: 10/04/99 14:40 PROSECTOR: LYNN HORNER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4239 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 74 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92218 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 382.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:15 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: NOT OBSERVED -MASS(ES); RIGHT: SINGLE MASS, 8 X 8 X -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >APPEARANCE; MISSING; TEETH; VERIFIED 4 MM, TAN AND FIRM; SAME ON CUT FOCAL AT NECROPSY: OBSERVED SURFACE; COLLECTED INTACT; REGIONAL LN -MINERALIZATION, PELVIC,-MINIMAL, FOCAL >SKIN & PELAGE; SORE/SCAB; PAW-HIND- (RENAL) NORMAL (CAP 22) -M-CARCINOMA, TUBULAR CELL,-NEOPLASM RIGHT; VERIFIED AT NECROPSY: OBSERVED PRESENT >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 12 X 10 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GRAY AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -EDEMA,-MODERATE, FOCAL DARK BROWN ERODED AREAS, UP TO 4 X 2 -EROSION,-MODERATE, FOCAL MM 4240 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 75 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92218 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 382.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:15 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,- MODERATE, DIFFUSE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: -EROSION,-SEVERE, FOCAL SINGLE DARK RED, CRUSTED AREA, 8 MM IN -HEMORRHAGE,-MODERATELY SEVERE, FOCAL DIAMETER; COLLECTED -INFLAMMATION, ACUTE,-MODERATE, FOCAL MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DRY, RED MATERIAL -ANIMAL THIN -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN AT BASE -------------------------------------------- 4241 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 76 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92218 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 382.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:15 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), EPIDIDYMIDES (EP), LN, RENAL (RL), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4242 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 77 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92219 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/03/99 STUDY DAY OF DEATH: 607 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 990.3 GRAMS DATE AND TIME OF NECROPSY: 12/03/99 15:08 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: NOT APPLICABLE -LARGE PELVIS(ES); BILATERAL -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >SKIN & PELAGE; SORE/SCAB; TAIL-DISTAL; -DIFFUSELY LIGHT; BILATERAL: TAN FOCAL VERIFIED AT NECROPSY: OBSERVED -DILATATION, PELVIC,-PRESENT >BEHAVIOR; OTHER; ANIMAL IS CHEWING ON LIVER (LI) : LIVER (LI) : DISTAL PORTION OF TAIL; VERIFIED AT -MOTTLED; ALL LOBES: LIGHT BROWN AND -DEGENERATION, CYSTIC,-MINIMAL, FOCAL NECROPSY: NOT APPLICABLE TAN -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, MULTI- FOCAL PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- SLIGHT, FOCAL -HEMORRHAGE,-MODERATE, FOCAL PITUITARY (PI) : >UNREMARKABLE 4243 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 78 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92219 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/03/99 STUDY DAY OF DEATH: 607 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 990.3 GRAMS DATE AND TIME OF NECROPSY: 12/03/99 15:08 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT POSTERIOR ASPECT: SINGLE DARK RED AREA, 1 MM IN DIAMETER STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE >UNREMARKABLE URINARY BLADDER (UB) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI- FOCAL PROSTATE (PR) : PROSTATE (PR) : -LARGE; 28 X 25 X 10 MM >UNREMARKABLE -DIFFUSELY RED; DARK RED SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 30 X 15 X 8 -EDEMA,-MODERATE, MULTI-FOCAL MM; BOTH ARE FILLED WITH A DARK RED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FLUID FOCAL DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT TAIL (TI) : TAIL (TI) : -CRUSTED AREA(S); DISTAL ONE THIRD: -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, DARK RED AND TAN INDISTINCT AREAS; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -URINARY BLADDER-LARGE LUMEN; UP TO 20 MM IN DIAMETER; FILLED WITH A DARK RED FLUID -ANIMAL OBESE 4244 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 79 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92219 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/03/99 STUDY DAY OF DEATH: 607 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 990.3 GRAMS DATE AND TIME OF NECROPSY: 12/03/99 15:08 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -GI TRACT-DARK FLUID CONTENT; ILEUM, JEJUNUM, CECUM AND COLON: DARK GREEN SEMIFLUID -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4245 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 80 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92220 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/25/99 STUDY DAY OF DEATH: 538 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/25/99 14:15 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- SEVERE, DIFFUSE LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PARATHYROID (PT) : PARATHYROID (PT) : -LARGE; BILATERAL: EACH IS 1 X 1 X 2 -HYPERPLASIA,-MODERATE, DIFFUSE MM HEART (HT) : -DEGENERATION/NECROSIS,-MODERATE, MULTI- FOCAL -MINERALIZATION,-SLIGHT, MULTI-FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL STOMACH, GL (ST) : -EROSION,-SLIGHT, FOCAL 4246 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 81 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92220 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/25/99 STUDY DAY OF DEATH: 538 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/25/99 14:15 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -MINERALIZATION,-MODERATELY SEVERE, MULTIPLE DARK BROWN AREAS, UP TO 1 X 3 MULTI-FOCAL MM JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC DEATH COMMENT (DC) : -CHRONIC PROGRESSIVE NEPHROPATHY,- PRESENT AORTA (AO) : AORTA (AO) : -LARGE; DIFFUSE: UP TO 5 MM IN -MINERALIZATION,-MODERATELY SEVERE, DIAMETER (CAP 19) MULTI-FOCAL -FIRM; DIFFUSE (CAP 19) GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL -AUTOLYSIS-SEVERE -DISCHARGE-OCULAR; LEFT: DRY RED MATERIAL -DISCHARGE-NASAL; DRY RED MATERIAL -------------------------------------------- 4247 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 82 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92220 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/25/99 STUDY DAY OF DEATH: 538 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/25/99 14:15 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4248 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 83 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92221 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 368.2 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 12:36 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: OBSERVED -LARGE PELVIS(ES); BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EXCRETION; FEW FECES; VERIFIED AT -ROUGH SURFACE, DIFFUSE; BILATERAL SEVERE, DIFFUSE NECROPSY: NOT APPLICABLE -DIFFUSELY LIGHT; BILATERAL: TAN >APPEARANCE; HUNCHED; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -MASS(ES); RIGHT MEDIAN LOBE: SINGLE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >BEHAVIOR; HYPOACTIVE; VERIFIED AT MASS, 25 X 20 X 15 MM, RED, DARK RED, FOCAL NECROPSY: NOT APPLICABLE TAN, AND FIRM; SAME ON CUT SURFACE; -VACUOLATION, HEPATOCELLULAR,-SLIGHT, >EYES; SQUINTED; PERI-ORBITAL; VERIFIED COLLECTED ON ROUTINE SECTION; REGIONAL MULTI-FOCAL AT NECROPSY: NOT APPLICABLE LN (HEPATIC) NORMAL (CAP 19) -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM >EYES; RED DISCHARGE; EYES; VERIFIED AT PRESENT NECROPSY: OBSERVED PARATHYROID (PT) : -HYPERPLASIA,-MODERATE, DIFFUSE TESTIS (TE) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL EYE (EY) : -INFLAMMATION, ACUTE, CORNEAL,- MODERATE, MULTI-FOCAL -VASCULARIZATION, CORNEAL,-MODERATE, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL 4249 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 84 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92221 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 368.2 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 12:36 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL DEATH COMMENT (DC) : -HEPATOCELLULAR ADENOMA,-PRESENT LN, OTHER (LN) : >UNREMARKABLE >NOTE:>HEPATIC LYMPH NODE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -------------------------------------------- 4250 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 85 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92221 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 368.2 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 12:36 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4251 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 86 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92222 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 802.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:35 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 95; 15 X 10MM KIDNEY (KD) : ANTERIOR 10 X 10MM POSTERIOR; VERIFIED -NEPHROPATHY, CHRONIC PROGRESSIVE,- AT NECROPSY: OBSERVED MODERATE, MULTI-FOCAL LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- MULTIPLE DARK RED AREAS, UP TO 4 X 3 FOCAL MM; COLLECTED ON ROUTINE SECTIONS PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -HYPERPLASIA, C-CELL,-MODERATE, FOCAL SKIN (SK) : SKIN (SK) : -MASS(ES); DORSAL LUMBAR REGION: TWO -CYST, EPIDERMAL INCLUSION,-PRESENT MASSES, ONE IS 23 X 17 X 9 MM, THE -M-CARCINOMA, SQUAMOUS CELL,-NEOPLASM OTHER IS 12 X 12 X 6 MM; BOTH ARE TAN PRESENT AND FIRM; BOTH ARE SAME ON CUT SURFACE; BOTH COLLECTED; REGIONAL LN (LUMBAR) NORMAL (CAP 22) NERVE, SCIATIC (SN) : >TISSUE MISSING HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI- FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL 4252 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 87 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92222 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 802.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:35 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 5 X 4 X 3 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4253 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 88 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92222 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 802.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:35 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, LUMBAR/ILIAC (LM), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4254 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 89 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92223 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 656.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:23 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0314 .0048 % .0115 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0327 .0050 % .0119 WEIGHT TAKEN BRAIN (BR) 2.7369 .4170 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.5158 .3833 % .9192 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.4904 .3794 % .9099 WEIGHT TAKEN LIVER (LI) 23.3120 3.5515 % 8.5177 WEIGHT TAKEN LUNG (LU) 2.3498 .3580 % .8586 WEIGHT TAKEN SPLEEN (SP) 1.0939 .1667 % .3997 WEIGHT TAKEN LEFT TESTIS (TE0) 1.5705 .2393 % .5738 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.5425 .2350 % .5636 WEIGHT TAKEN LF THYROID/PARA (TT0) .0169 .0026 % .0062 WEIGHT TAKEN RT THYROID/PARA (TT1) .0186 .0028 % .0068 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE -BONE MARROW SMEAR TAKEN -------------------------------------------- 4255 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 90 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92223 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 656.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:23 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4256 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 91 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92224 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 716 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/21/00 8:04 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; LUNG (LU) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, VASCULAR,-MINIMAL, >WART LIKE LESION(S); DORSAL; WEEK 93; FOCAL VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : >APPEARANCE; SWOLLEN; PAWS-HIND; -CAST, PROTEINACEOUS,-MINIMAL, MULTI- VERIFIED AT NECROPSY: OBSERVED FOCAL >SKIN & PELAGE; BROWN HAIR COAT; SKIN (SK) : SKIN (SK) : VENTRAL-ABDOMINAL; VERIFIED AT -CRUSTED AREA(S); DORSAL THORACIC -B-BASAL CELL TUMOR,-NEOPLASM PRESENT NECROPSY: OBSERVED REGION: SINGLE RED, TAN, DRY, FIRM, RAISED AREA, 13 X 6 X 6 MM (CAP 23) HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -RED FOCUS(I)/AREA(S); RIGHT: SINGLE >UNREMARKABLE DARK RED AREA, 3 MM IN DIAMETER ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT PITUITARY (PI) : PITUITARY (PI) : -DIFFUSELY RED; LEFT ANTERIOR ASPECT: -B-ADENOMA,-NEOPLASM PRESENT SINGLE DARK RED AREA, 2 MM IN DIAMETER JEJUNUM (JE) : >AUTOLYTIC 4257 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 92 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92224 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 716 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/21/00 8:04 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL, -EDEMA,-MODERATE, MULTI-FOCAL HINDFEET: SINGLE RED, TAN MOIST -EROSION,-SEVERE, MULTI-FOCAL ABRADED AREA ON EACH, LEFT IS 20 X 15 -EXOSTOSES,-MODERATE, MULTI-FOCAL MM, RIGHT IS 12 X 9 MM; BOTH APPEAR -INFLAMMATION, ACUTE,-SEVERE, MULTI- SWOLLEN; BOTH COLLECTED FOCAL LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL, LUMBAR: EACH IS 13 -DILATATION, SINUSOIDAL,-SLIGHT, MULTI- X 6 X 4 MM (CAP 22) FOCAL GENERAL COMMENT (GC) : -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: BROWN AND MOIST -------------------------------------------- 4258 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 93 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92224 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 716 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/21/00 8:04 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4259 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 94 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92225 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/12/99 STUDY DAY OF DEATH: 494 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 447.4 GRAMS DATE AND TIME OF NECROPSY: 08/12/99 14:45 PROSECTOR: DENNIS HOFFMAN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >SKIN & PELAGE; ROUGH HAIR COAT; FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE LIVER (LI) : >SKIN & PELAGE; BROWN HAIR COAT; -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- PERINEAL AREA; VERIFIED AT NECROPSY: FOCAL OBSERVED -VACUOLATION, HEPATOCELLULAR,-MODERATE, >BEHAVIOR; ATAXIC; VERIFIED AT MULTI-FOCAL NECROPSY: NOT APPLICABLE THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- SLIGHT, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 8 X 11 MM, -B-ADENOMA,-NEOPLASM PRESENT SOFT, RED, DARK RED, AND TAN HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL STOMACH, NONGL (SU) : -EDEMA,-MODERATE, FOCAL 4260 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 95 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92225 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/12/99 STUDY DAY OF DEATH: 494 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 447.4 GRAMS DATE AND TIME OF NECROPSY: 08/12/99 14:45 PROSECTOR: DENNIS HOFFMAN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; DARK BROWN AND MOIST -DISCHARGE-OCULAR; DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 4261 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 96 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92225 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/12/99 STUDY DAY OF DEATH: 494 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 447.4 GRAMS DATE AND TIME OF NECROPSY: 08/12/99 14:45 PROSECTOR: DENNIS HOFFMAN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4262 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 97 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92226 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 496.8 GRAMS DATE AND TIME OF NECROPSY: 12/27/99 9:56 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >RESPIRATION; IRREGULAR; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); AT JUNCTION -DEGENERATION, CYSTIC,-MODERATE, MULTI- OF MEDIAN LOBES: SINGLE TAN AREA, 3 X FOCAL 2 MM (CAP 19) -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-MODERATELY SEVERE, FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 14 X 10 X 10 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL 4263 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 98 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92226 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 496.8 GRAMS DATE AND TIME OF NECROPSY: 12/27/99 9:56 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; DARK RED AND MOIST -DISCHARGE-OCULAR; RIGHT: DARK RED AND DRY -ANIMAL THIN -------------------------------------------- 4264 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 99 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92226 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 496.8 GRAMS DATE AND TIME OF NECROPSY: 12/27/99 9:56 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4265 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 100 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92227 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/17/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 630.9 GRAMS DATE AND TIME OF NECROPSY: 03/17/00 8:00 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT LUNG (LU) : NECROPSY: NOT OBSERVED -INFLAMMATION, CHRONIC,-SLIGHT, FOCAL >SKIN & PELAGE; PALE; ENTIRE BODY; KIDNEY (KD) : KIDNEY (KD) : VERIFIED AT NECROPSY: NOT APPLICABLE -CYST(S); LEFT, CORTICAL SURFACE: -CYST,-MODERATE, FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT SINGLE CLEAR FLUID FILLED CYST, 5 X 3 -DILATATION, PELVIC,-PRESENT NECROPSY: NOT APPLICABLE X 2 MM -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EXCRETION; FEW FECES; VERIFIED AT SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE -PYELONEPHRITIS,-SLIGHT, MULTI-FOCAL >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -HEMATOPOIESIS, EXTRAMEDULLARY,- MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, MULTI- FOCAL BONE, FEMUR (FE) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT HEART (HT) : -FIBROSIS,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 4266 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 101 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92227 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/17/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 630.9 GRAMS DATE AND TIME OF NECROPSY: 03/17/00 8:00 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, DARK RED, -CYST,-PRESENT TAN AND SOFT -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: -EDEMA,-MODERATE, MULTI-FOCAL MULTIPLE DARK RED AREAS, UP TO 12 X 3 -INFLAMMATION, ACUTE,-MODERATE, MULTI- MM FOCAL -THICKENED WALL; DIFFUSE DUODENUM (DU) : DUODENUM (DU) : -RED FOCUS(I)/AREA(S); MUCOSA: -HEMORRHAGE,-MODERATE, MULTI-FOCAL MULTIPLE DARK RED AREAS, UP TO 7 X 3 -INFLAMMATION, ACUTE,-MODERATELY MM; COLLECTED ON ROUTINE SECTION SEVERE, MULTI-FOCAL -ULCERATION,-MODERATELY SEVERE, MULTI- FOCAL JEJUNUM (JE) : JEJUNUM (JE) : -RED FOCUS(I)/AREA(S); MUCOSA: -HEMORRHAGE,-MODERATE, MULTI-FOCAL MULTIPLE DARK RED AREAS, UP TO 7 X 5 -INFLAMMATION, ACUTE,-MODERATELY MM; COLLECTED ON ROUTINE SECTION SEVERE, MULTI-FOCAL -ULCERATION,-MODERATELY SEVERE, MULTI- FOCAL ILEUM (IL) : -EROSION,-MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI- FOCAL CECUM (CE) : CECUM (CE) : -THICKENED WALL; DIFFUSE -EDEMA,-MODERATELY SEVERE, MULTI-FOCAL -RED FOCUS(I)/AREA(S); MUCOSA: SINGLE -INFLAMMATION, ACUTE,-MODERATELY AREA, 12 X 10 MM SEVERE, MULTI-FOCAL 4267 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 102 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92227 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/17/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 630.9 GRAMS DATE AND TIME OF NECROPSY: 03/17/00 8:00 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CECUM (CE) : -ULCERATION,-MODERATELY SEVERE, MULTI- FOCAL COLON (CO) : -EDEMA,-MINIMAL, FOCAL URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,- MINIMAL, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE, MULTI-FOCAL DEATH COMMENT (DC) : -ENTERITIS,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 4268 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 103 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92227 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/17/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 630.9 GRAMS DATE AND TIME OF NECROPSY: 03/17/00 8:00 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), ILEUM (IL), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), PARATHYROID (PT), TESTIS (TE), SKIN (SK), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), RECTUM (RE), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4269 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 104 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92228 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/25/99 STUDY DAY OF DEATH: 446 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 423.5 GRAMS DATE AND TIME OF NECROPSY: 06/25/99 10:32 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE THYMUS (TH) : >APPEARANCE; HUNCHED; VERIFIED AT >SECTION EXAMINED; TISSUE NOT PRESENT NECROPSY: NOT APPLICABLE PITUITARY (PI) : PITUITARY (PI) : >RESPIRATION; LABORED; VERIFIED AT -MASS(ES); 13 X 10 X 7 MM, TAN, RED, -B-ADENOMA,-NEOPLASM PRESENT NECROPSY: NOT APPLICABLE VASCULAR AND FIRM >BEHAVIOR; ATAXIC; VERIFIED AT PROSTATE (PR) : NECROPSY: NOT APPLICABLE -INFLAMMATION, ACUTE,-SLIGHT, FOCAL >SKIN & PELAGE; RED HAIR COAT; VENTRAL- DEATH COMMENT (DC) : ABDOMINAL; VERIFIED AT NECROPSY: -PITUITARY ADENOMA,-PRESENT OBSERVED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 4270 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92228 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/25/99 STUDY DAY OF DEATH: 446 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 423.5 GRAMS DATE AND TIME OF NECROPSY: 06/25/99 10:32 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4271 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 106 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92229 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/26/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/26/99 8:00 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; DORSAL; KIDNEY (KD) : KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -LARGE; BILATERAL: EACH IS 33 X 20 X -NEPHROPATHY, CHRONIC PROGRESSIVE,- 17 MM MODERATE, MULTI-FOCAL LIVER (LI) : LIVER (LI) : -MASS(ES); ATTACHED TO MARGIN OF LEFT -CYST,-PRESENT LATERAL LOBE: SINGLE MASS, 30 X 25 X -HEMORRHAGE,-MODERATE, MULTI-FOCAL 15 MM, DARK RED, TAN AND SOFT; SAME ON -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM CUT SURFACE; COLLECTED; REGIONAL LN PRESENT (HEPATIC) NOT IDENTIFIED THYROID (TY) : THYROID (TY) : -LARGE; BILATERAL: EACH IS 7 X 5 X 4 >UNREMARKABLE MM SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); RIGHT DORSAL LUMBAR -CYST, EPIDERMAL INCLUSION,-PRESENT REGION: SINGLE TAN AREA, 3 X 2 MM (CAP 19) HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -RAISED AREA(S); RIGHT POSTERIOR -B-ADENOMA,-NEOPLASM PRESENT ASPECT: SINGLE RED AREA, 3 X 2 X 2 MM 4272 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 107 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92229 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/26/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/26/99 8:00 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ILEUM (IL) : >AUTOLYTIC DEATH COMMENT (DC) : -HEPATOCELLULAR ADENOMA,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL SINGLE MASS, 20 X 15 X 13 MM, DARK -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- RED, RED, TAN AND SOFT; CUT SURFACE FOCAL EXUDES A LIGHT GREEN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -------------------------------------------- 4273 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 108 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92229 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/26/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/26/99 8:00 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4274 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 109 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92230 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 731.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 9:46 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0289 .0040 % .0123 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0255 .0035 % .0109 WEIGHT TAKEN BRAIN (BR) 2.3457 .3209 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.6137 .3576 % 1.1143 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.5839 .3535 % 1.1015 WEIGHT TAKEN LIVER (LI) 27.4278 3.7521 % 11.6928 WEIGHT TAKEN LUNG (LU) 2.3634 .3233 % 1.0075 WEIGHT TAKEN SPLEEN (SP) .9887 .1353 % .4215 WEIGHT TAKEN LEFT TESTIS (TE0) 1.8038 .2468 % .7690 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.7845 .2441 % .7608 WEIGHT TAKEN LF THYROID/PARA (TT0) .0214 .0029 % .0091 WEIGHT TAKEN RT THYROID/PARA (TT1) .0196 .0027 % .0084 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -MOTTLED; ALL LOBES: RED, BROWN AND -HEMORRHAGE,-SLIGHT, MULTI-FOCAL TAN -NECROSIS, HEPATOCELLULAR,-MODERATELY SEVERE, MULTI-FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 4275 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 110 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92230 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 731.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 9:46 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4276 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 111 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92231 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 801.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:01 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; VENTRAL- KIDNEY (KD) : THORACIC-RIGHT; VERIFIED AT NECROPSY: -NEPHROPATHY, CHRONIC PROGRESSIVE,- OBSERVED MODERATE, MULTI-FOCAL LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- MULTIPLE DARK RED AREAS, UP TO 3 MM IN FOCAL DIAMETER; COLLECTED ON ROUTINE SECTIONS PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL -HYPERPLASIA, ISLET CELLS,-MODERATE, FOCAL SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); VENTRAL THORACIC -B-ADENOMA, BASAL CELL,-NEOPLASM REGION: SINGLE RED, DRY CRUSTED AREA, PRESENT 2 MM IN DIAMETER (CAP 22) HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -HYPERTROPHY,-SLIGHT, FOCAL -VACUOLATION,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HYPERPLASIA,-MODERATE, MULTI-FOCAL 4277 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 112 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92231 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 801.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:01 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4278 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 113 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92231 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 801.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:01 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4279 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 114 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92232 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 624.7 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 13:54 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NO FECES; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: NOT APPLICABLE -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- >SKIN & PELAGE; RED HAIR COAT; HEAD- SEVERE, DIFFUSE ENTIRE; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; ROUGH HAIR COAT; -DIFFUSELY LIGHT; ALL LOBES: TAN -VACUOLATION, HEPATOCELLULAR,-SEVERE, VERIFIED AT NECROPSY: NOT APPLICABLE DIFFUSE PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL -HYPERTROPHY, VASCULAR,-MODERATE, FOCAL THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, MULTI-FOCAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 17 X 10 X -MINERALIZATION,-MINIMAL, FOCAL 10 MM BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL SPLEEN (SP) : SPLEEN (SP) : -ROUGH SURFACE; DIFFUSE >UNREMARKABLE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- SLIGHT, FOCAL 4280 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 115 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92232 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 624.7 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 13:54 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -VACUOLATION,-MODERATE, FOCAL PITUITARY (PI) : PITUITARY (PI) : -CYST(S); SINGLE CLEAR FLUID FILLED -B-ADENOMA,-NEOPLASM PRESENT CYST, 6 MM IN DIAMETER STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE >UNREMARKABLE STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE -EDEMA,-MINIMAL, FOCAL -EROSION/ULCERATION; MUCOSA: MULTIPLE -INFLAMMATION, ACUTE,-MINIMAL, MULTI- TAN ERODED AREAS, UP TO 2 MM IN FOCAL DIAMETER PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, URETHRA: GREEN AND FIRM MULTI-FOCAL DEATH COMMENT (DC) : -CHRONIC PROGRESSIVE NEPHROPATHY,- PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; PERIANAL: MOIST BROWN MATERIAL -DISCHARGE-NASAL; DRY RED MATERIAL -STAINS - SKIN/HAIRCOAT; INVOLVING ENTIRE HEAD: DRY RED MATERIAL -------------------------------------------- 4281 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 116 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92232 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 624.7 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 13:54 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4282 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 117 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92233 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 373.9 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:40 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EYES; SQUINTED; PERI-ORBITAL; VERIFIED SLIGHT, MULTI-FOCAL AT NECROPSY: NOT APPLICABLE LIVER (LI) : >SKIN & PELAGE; YELLOW HAIR COAT; -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- VERIFIED AT NECROPSY: OBSERVED FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, FOCAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 8 X 9 X 18 -ATROPHY/DEGENERATION,-MODERATELY MM SEVERE, MULTI-FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 7 X 9 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL FIRM, DARK RED AND TAN -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL 4283 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 118 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92233 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 373.9 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:40 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL SINGLE MASS, 10 X 17 X 26 MM, FIRM, -B-FIBROADENOMA,-NEOPLASM PRESENT LOBULATED, TAN AND RED; ON SECTION SOME AREAS EXUDE TAN CREAMY SEMIFLUID (CAP 22); REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; MOIST, YELLOW MATERIAL -ANIMAL THIN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4284 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 119 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92233 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 373.9 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:40 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4285 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 120 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92234 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 681.0 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 14:25 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- KIDNEY (KD) : LEFT; VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >SKIN & PELAGE; BROWN HAIR COAT; FOCAL VENTRAL-ABDOMINAL; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >EYES; SQUINTED; PERI-ORBITAL; VERIFIED FOCAL AT NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR,-MODERATE, >APPEARANCE; THIN; VERIFIED AT MULTI-FOCAL NECROPSY: NOT OBSERVED PANCREAS (PA) : >EXCRETION; NO FECES; VERIFIED AT -B-ADENOMA, ISLET CELL,-NEOPLASM NECROPSY: NOT APPLICABLE PRESENT BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT THYMUS (TH) : -CYST,-PRESENT -HEMORRHAGE,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MODERATE, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 5 X 5 MM, RED, TAN AND -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE -EDEMA,-MODERATE, FOCAL -FIBROSIS,-MODERATE, FOCAL 4286 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 121 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92234 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 681.0 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 14:25 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE -INFLAMMATION, ACUTE,-SLIGHT, FOCAL TAN ERODED AREA, 4 X 2 MM -ULCERATION,-MODERATELY SEVERE, FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); LEFT -EROSION,-SEVERE, FOCAL HINDFOOT: SINGLE DARK RED, MOIST -FIBROSIS,-MODERATE, FOCAL ABRADED AREA, 16 X 12 MM; COLLECTED -INFLAMMATION, ACUTE,-SEVERE, FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; YELLOW AND BROWN MATERIAL -------------------------------------------- 4287 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 122 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92234 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 681.0 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 14:25 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4288 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 123 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92235 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/11/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 601.3 GRAMS DATE AND TIME OF NECROPSY: 02/11/00 14:48 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >APPEARANCE; SWOLLEN; NOSE; VERIFIED AT FOCAL NECROPSY: OBSERVED -INFARCT,-MODERATE, FOCAL >RESPIRATION; AUDIBLE; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >RESPIRATION; LABORED; VERIFIED AT SURFACE OF LEFT LATERAL LOBE: SINGLE FOCAL NECROPSY: NOT APPLICABLE DARK RED AREA, 3 MM IN DIAMETER; >SKIN & PELAGE; BROWN HAIR COAT; COLLECTED ON ROUTINE SECTION PERINEAL AREA; VERIFIED AT NECROPSY: SKIN (SK) : SKIN (SK) : OBSERVED -SUBCUTANEOUS EDEMA; SURROUNDING -EDEMA,-MODERATE, FOCAL NARES: SMALL AMOUNT OF CLEAR AND -HEMORRHAGE,-SLIGHT, FOCAL GELATINOUS EDEMA (CAP 20) -INFLAMMATION, ACUTE,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT 4289 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 124 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92235 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/11/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 601.3 GRAMS DATE AND TIME OF NECROPSY: 02/11/00 14:48 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 19) -HEMORRHAGE,-SLIGHT, MULTI-FOCAL CALVARIUM/SKULL (CA) : CALVARIUM/SKULL (CA) : -RED FOCUS(I)/AREA(S); ROSTRAL TO -HEMORRHAGE,-MODERATE, MULTI-FOCAL EYES: SINGLE DARK RED AREA, 15 X 10 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; BROWN, RED AND DRY MATERIAL -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -------------------------------------------- 4290 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 125 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92235 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/11/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 601.3 GRAMS DATE AND TIME OF NECROPSY: 02/11/00 14:48 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4291 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 126 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92236 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/20/99 8:42 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EYES; PALE; EYES; VERIFIED AT SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE LIVER (LI) : >RESPIRATION; IRREGULAR; VERIFIED AT -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >APPEARANCE; THIN; VERIFIED AT THYROID (TY) : NECROPSY: NOT OBSERVED -CYST, FOLLICULAR,-MINIMAL, MULTI-FOCAL EYE (EY) : -INFLAMMATION, ACUTE, CORNEAL,-SLIGHT, MULTI-FOCAL -VASCULARIZATION, CORNEAL,-MODERATE, MULTI-FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 6 MM, DARK RED, -HEMORRHAGE,-MINIMAL, MULTI-FOCAL GRAY, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL ILEUM (IL) : >AUTOLYTIC 4292 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 127 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92236 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/20/99 8:42 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING >UNREMARKABLE URETHRA: GREEN AND FIRM DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -DISCHARGE-NASAL; DARK RED AND DRY -------------------------------------------- 4293 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 128 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92236 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/20/99 8:42 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), MAMMARY, MALE (MM) ------------------------------------------------------------------------------------------------------------------------------------ 4294 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 129 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92237 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/11/00 STUDY DAY OF DEATH: 706 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 607.6 GRAMS DATE AND TIME OF NECROPSY: 03/11/00 15:43 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- KIDNEY (KD) : LEFT; VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >EYES; RED DISCHARGE; EYES; VERIFIED AT FOCAL NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, MULTI- >DISCHARGE; NASAL; RED IN COLOR; FOCAL VERIFIED AT NECROPSY: NOT OBSERVED LIVER (LI) : LIVER (LI) : >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -MOTTLED; ALL LOBES: DARK RED AND TAN -VACUOLATION, HEPATOCELLULAR,-SEVERE, AT NECROPSY: NOT APPLICABLE DIFFUSE >APPEARANCE; THIN; VERIFIED AT PANCREAS (PA) : NECROPSY: NOT OBSERVED -PIGMENT,-MINIMAL, MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; NO FECES; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE PITUITARY (PI) : PITUITARY (PI) : >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED -MASS(ES); 12 X 8 X 6 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- AT NECROPSY: NOT APPLICABLE RED, TAN AND SOFT FOCAL >RESPIRATION; LABORED; VERIFIED AT -B-ADENOMA,-NEOPLASM PRESENT NECROPSY: NOT APPLICABLE STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE >UNREMARKABLE STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE -EDEMA,-MINIMAL, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL 4295 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 130 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92237 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/11/00 STUDY DAY OF DEATH: 706 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 607.6 GRAMS DATE AND TIME OF NECROPSY: 03/11/00 15:43 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: -INFLAMMATION, ACUTE,-MODERATE, FOCAL SINGLE DRY BROWN CRUSTED AREA, 10 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: MOIST DARK BROWN MATERIAL -------------------------------------------- 4296 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 131 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92237 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/11/00 STUDY DAY OF DEATH: 706 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 607.6 GRAMS DATE AND TIME OF NECROPSY: 03/11/00 15:43 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4297 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 132 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92238 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 775.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 11:21 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0322 .0042 % .0133 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0351 .0045 % .0145 WEIGHT TAKEN BRAIN (BR) 2.4208 .3120 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.4709 .3185 % 1.0207 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.4580 .3168 % 1.0154 WEIGHT TAKEN LIVER (LI) 20.9165 2.6958 % 8.6403 WEIGHT TAKEN LUNG (LU) 2.0432 .2633 % .8440 WEIGHT TAKEN SPLEEN (SP) 1.0204 .1315 % .4215 WEIGHT TAKEN LEFT TESTIS (TE0) 1.8447 .2377 % .7620 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.8528 .2388 % .7654 WEIGHT TAKEN LF THYROID/PARA (TT0) .0218 .0028 % .0090 WEIGHT TAKEN RT THYROID/PARA (TT1) .0264 .0034 % .0109 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, MULTI- FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE -BONE MARROW SMEAR TAKEN -------------------------------------------- 4298 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 133 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92238 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 775.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 11:21 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4299 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 134 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92239 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 474.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:17 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; BROWN HAIR COAT; KIDNEY (KD) : VENTRAL-ABDOMINAL; VERIFIED AT -CAST, PROTEINACEOUS,-MINIMAL, MULTI- NECROPSY: OBSERVED FOCAL >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- >EYES; SQUINTED; PERI-ORBITAL; VERIFIED FOCAL AT NECROPSY: NOT APPLICABLE BRAIN (BR) : BRAIN (BR) : >APPEARANCE; THIN; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT OBSERVED -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; SORE/SCAB; PAW-HIND- HEART (HT) : LEFT; VERIFIED AT NECROPSY: OBSERVED -DEGENERATION/NECROSIS,-MINIMAL, MULTI- >APPEARANCE; HEAD TILT; RIGHT; VERIFIED FOCAL AT NECROPSY: NOT APPLICABLE -FIBROSIS,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT ADRENAL, MEDULLA (MA) : -ONE EXAMINED,-PRESENT -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, GRAY, RED -B-ADENOMA,-NEOPLASM PRESENT AND SOFT PROSTATE (PR) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL 4300 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 135 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92239 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 474.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:17 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: -FIBROSIS,-MODERATELY SEVERE, FOCAL SINGLE DARK RED, DRY CRUSTED AREA, 6 -INFLAMMATION, ACUTE,-SLIGHT, FOCAL MM IN DIAMETER; COLLECTED HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL LN, MEDIASTINAL (ML) : -DILATATION, SINUSOIDAL,-MODERATE, FOCAL >NOTE:>MEDIASTINAL LYMPH NODE PRESENT ON THYMUS SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; EXTENDING TO MID ABDOMINAL REGION: BROWN, DRY MATERIAL -BONE MARROW SMEAR TAKEN -------------------------------------------- 4301 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 136 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92239 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 474.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:17 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) ------------------------------------------------------------------------------------------------------------------------------------ 4302 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 137 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92240 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/04/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/04/99 13:13 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- KIDNEY (KD) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- MULTIPLE DARK RED FOCI, 3 MM IN FOCAL DIAMETER; COLLECTED ON ROUTINE -VACUOLATION, HEPATOCELLULAR,-SLIGHT, SECTIONS MULTI-FOCAL SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MODERATE, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: -EDEMA,-MODERATE, FOCAL SINGLE DRY, DARK RED CRUSTED AREA, 13 -EROSION,-MODERATELY SEVERE, FOCAL X 8 MM; SURROUNDING TISSUE APPEARS -FIBROSIS,-MODERATELY SEVERE, FOCAL SWOLLEN; COLLECTED -INFLAMMATION, ACUTE,-MODERATE, FOCAL 4303 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 138 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92240 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/04/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/04/99 13:13 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); RIGHT VENTRAL CERVICAL >TISSUE MISSING REGION: SINGLE MASS, 30 X 23 X 12 MM, >NOTE:>MAMMARY MASS DESCRIBED GROSSLY RED, TAN, LOBULATED AND FIRM; SAME ON WAS A SUBCUTANEOUS FIBROMA CUT SURFACE; COLLECTED INTACT; REGIONAL LN (MANDIBULAR) NORMAL (CAP 19) GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- 4304 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 139 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92240 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/04/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/04/99 13:13 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4305 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 140 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92241 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 669.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 12:59 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HEAD TILT; LEFT; VERIFIED KIDNEY (KD) : KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -LARGE PELVIS(ES); BILATERAL -INFARCT,-MODERATE, MULTI-FOCAL >EYES; RED DISCHARGE; EYE-LEFT; -NEPHROPATHY, CHRONIC PROGRESSIVE,- VERIFIED AT NECROPSY: OBSERVED MODERATE, DIFFUSE >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -MOTTLED; ALL LOBES: BROWN AND TAN -VACUOLATION, HEPATOCELLULAR,-MODERATE, >SKIN & PELAGE; ROUGH HAIR COAT; MULTI-FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT, DIFFUSE EYE (EY) : -INFLAMMATION, ACUTE, CORNEAL,-MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE, CILIARY,-SLIGHT, MULTI-FOCAL HEART (HT) : HEART (HT) : -MASS(ES); TRIMMING COMMENT: WITHIN -DEGENERATION/NECROSIS,-SLIGHT, MULTI- RIGHT ATRIUM: SINGLE MASS, 3 MM IN FOCAL DIAMETER, TAN AND FIRM; SUBMITTED WITH -FIBROSIS,-SLIGHT, MULTI-FOCAL ROUTINE SECTION -THROMBUS, ATRIAL,-MODERATELY SEVERE, FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -RAISED AREA(S); LEFT ANTERIOR ASPECT: -B-ADENOMA,-NEOPLASM PRESENT SINGLE TAN AREA, 3 X 2 X 2 MM 4306 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 141 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92241 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 669.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 12:59 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL DEATH COMMENT (DC) : -ATRIAL THROMBUS,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; -HEMORRHAGE,-MINIMAL, MULTI-FOCAL COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DARK RED AND DRY -------------------------------------------- 4307 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 142 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92241 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 669.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 12:59 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4308 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 143 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92242 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/14/99 STUDY DAY OF DEATH: 465 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 576.2 GRAMS DATE AND TIME OF NECROPSY: 07/14/99 15:30 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HUNCHED; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >APPEARANCE; THIN; VERIFIED AT FOCAL NECROPSY: NOT OBSERVED -PYELONEPHRITIS,-SLIGHT, FOCAL >EXCRETION; NO FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR,-MODERATE, >SKIN & PELAGE; BROWN HAIR COAT; MULTI-FOCAL PERINEAL AREA; VERIFIED AT NECROPSY: BRAIN (BR) : BRAIN (BR) : OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >RESPIRATION; IRREGULAR; VERIFIED AT THYMUS (TH) : NECROPSY: NOT APPLICABLE -CYST,-PRESENT >EYES; SQUINTED; PERI-ORBITAL-LEFT; PITUITARY (PI) : PITUITARY (PI) : VERIFIED AT NECROPSY: NOT APPLICABLE -MASS(ES); 15 X 10 X 10 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- >BEHAVIOR; HYPOACTIVE; VERIFIED AT TAN AND SOFT FOCAL NECROPSY: NOT APPLICABLE -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -EDEMA,-MODERATELY SEVERE, DIFFUSE DARK RED AND TAN ERODED AREAS, UP TO -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 16 X 7 MM -INFLAMMATION, ACUTE,-MODERATE, FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, ACUTE,-SLIGHT, MULTI- URETHRA: GREEN AND FIRM FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: CLEAR -SECRETION, INCREASED,-PRESENT 4309 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 144 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92242 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/14/99 STUDY DAY OF DEATH: 465 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 576.2 GRAMS DATE AND TIME OF NECROPSY: 07/14/99 15:30 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; BROWN AND MOIST -------------------------------------------- 4310 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 145 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92242 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/14/99 STUDY DAY OF DEATH: 465 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 576.2 GRAMS DATE AND TIME OF NECROPSY: 07/14/99 15:30 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4311 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 146 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92243 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/03/99 STUDY DAY OF DEATH: 485 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 611.3 GRAMS DATE AND TIME OF NECROPSY: 08/03/99 11:30 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HUNCHED; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >BEHAVIOR; HYPOACTIVE; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE PANCREAS (PA) : >RESPIRATION; LABORED; VERIFIED AT -PIGMENT,-MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE BRAIN (BR) : BRAIN (BR) : >SKIN & PELAGE; RED HAIR COAT; VENTRAL- -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT ABDOMINAL; VERIFIED AT NECROPSY: -DILATATION, VENTRICULAR,-PRESENT OBSERVED THYMUS (TH) : >SKIN & PELAGE; PALE; ENTIRE BODY; -CYST,-PRESENT VERIFIED AT NECROPSY: NOT OBSERVED ADRENAL, CORTEX (AC) : -HYPERTROPHY,-MODERATE, FOCAL -THROMBUS,-MODERATE, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 9 X 8 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED AND SOFT PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL REGION: DRY RED MATERIAL 4312 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 147 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92243 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/03/99 STUDY DAY OF DEATH: 485 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 611.3 GRAMS DATE AND TIME OF NECROPSY: 08/03/99 11:30 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4313 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 148 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92244 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/02/99 STUDY DAY OF DEATH: 453 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/02/99 10:20 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LUNG (LU) : LEFT; VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MODERATELY SEVERE, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: -EROSION,-SLIGHT, FOCAL SINGLE DARK RED AND DRY CRUSTED AREA, -INFLAMMATION, ACUTE,-SLIGHT, FOCAL 2 X 1 MM; COLLECTED GENERAL COMMENT (GC) : -DISCHARGE-NASAL; MOIST RED MATERIAL -------------------------------------------- 4314 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 149 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92244 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/02/99 STUDY DAY OF DEATH: 453 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/02/99 10:20 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4315 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 150 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92245 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/25/99 STUDY DAY OF DEATH: 476 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 482.0 GRAMS DATE AND TIME OF NECROPSY: 07/25/99 14:27 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EXCRETION; FEW FECES; VERIFIED AT MODERATE, MULTI-FOCAL NECROPSY: NOT APPLICABLE -PYELONEPHRITIS,-MODERATE, MULTI-FOCAL >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -DEPRESSED FOCUS(I)/AREA(S); VISCERAL -VACUOLATION, HEPATOCELLULAR,- >SKIN & PELAGE; YELLOW HAIR COAT; SURFACE OF RIGHT MEDIAN LOBE: SINGLE MODERATELY SEVERE, MULTI-FOCAL VERIFIED AT NECROPSY: OBSERVED TAN AND LINEAR AREA, 12 X 3 MM; COLLECTED ON ROUTINE SECTION BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : >NOTE:>TRIMMING COMMENT: ONE ADRENAL -ONE EXAMINED,-PRESENT MISSING AT TIME OF TRIMMING ADRENAL, MEDULLA (MA) : ADRENAL, MEDULLA (MA) : >NOTE:>TRIMMING COMMENT: ONE ADRENAL -ONE EXAMINED,-PRESENT MISSING AT TIME OF TRIMMING PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 9 X 8 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, TAN, LOBULATED AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA, NEAR >UNREMARKABLE PYLORIC VALVE: SINGLE TAN AND SLIGHTLY ERODED AREA, 3 X 2 MM 4316 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 151 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92245 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/25/99 STUDY DAY OF DEATH: 476 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 482.0 GRAMS DATE AND TIME OF NECROPSY: 07/25/99 14:27 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER AND LOWER INCISORS -ANIMAL THIN -STAINS - SKIN/HAIRCOAT; DIFFUSE: YELLOW -------------------------------------------- 4317 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 152 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92245 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/25/99 STUDY DAY OF DEATH: 476 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 482.0 GRAMS DATE AND TIME OF NECROPSY: 07/25/99 14:27 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4318 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 153 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92246 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 674.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:56 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0316 .0047 % .0133 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0328 .0049 % .0138 WEIGHT TAKEN BRAIN (BR) 2.3732 .3516 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.1678 .3212 % .9135 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.9672 .2915 % .8289 WEIGHT TAKEN LIVER (LI) 17.2687 2.5587 % 7.2765 WEIGHT TAKEN LUNG (LU) 2.1696 .3215 % .9142 WEIGHT TAKEN SPLEEN (SP) .9390 .1391 % .3957 WEIGHT TAKEN LEFT TESTIS (TE0) 1.7567 .2603 % .7402 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.7110 .2535 % .7210 WEIGHT TAKEN LF THYROID/PARA (TT0) .0161 .0024 % .0068 WEIGHT TAKEN RT THYROID/PARA (TT1) .0241 .0036 % .0102 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; DEATH COMMENT (DC) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE -BONE MARROW SMEAR TAKEN -------------------------------------------- 4319 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 154 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92246 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 674.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:56 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4320 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 155 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92247 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 884.8 GRAMS DATE AND TIME OF NECROPSY: 04/04/99 8:38 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0326 .0037 % .0119 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0416 .0047 % .0152 WEIGHT TAKEN BRAIN (BR) 2.7388 .3095 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.8337 .3203 % 1.0347 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.7175 .3071 % .9922 WEIGHT TAKEN LIVER (LI) 26.2003 2.9612 % 9.5663 WEIGHT TAKEN LUNG (LU) 2.3746 .2684 % .8670 WEIGHT TAKEN SPLEEN (SP) 1.6381 .1851 % .5981 WEIGHT TAKEN LEFT TESTIS (TE0) 1.9421 .2195 % .7091 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.9533 .2208 % .7132 WEIGHT TAKEN LF THYROID/PARA (TT0) .0380 .0043 % .0139 WEIGHT TAKEN RT THYROID/PARA (TT1) .0382 .0043 % .0139 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-SLIGHT, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : >NOTE:>TRIMMING COMMENT: NOT FOUND IN >TISSUE MISSING WET TISSUE AT TRIMMING DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE -BONE MARROW SMEAR TAKEN -------------------------------------------- 4321 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 156 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92247 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 884.8 GRAMS DATE AND TIME OF NECROPSY: 04/04/99 8:38 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4322 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 157 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92248 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/26/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 444.3 GRAMS DATE AND TIME OF NECROPSY: 07/26/99 13:55 PROSECTOR: JILL PAUS RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >SKIN & PELAGE; ROUGH HAIR COAT; MODERATELY SEVERE, DIFFUSE VERIFIED AT NECROPSY: NOT APPLICABLE BRAIN (BR) : BRAIN (BR) : >EXCRETION; FEW FECES; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE PITUITARY (PI) : PITUITARY (PI) : >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -MASS(ES); SINGLE MASS, 15 X 9 X 8 MM, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL AT NECROPSY: NOT APPLICABLE RED, TAN, SOFT AND LOBULATED -B-ADENOMA,-NEOPLASM PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT PROSTATE (PR) : NECROPSY: NOT APPLICABLE -INFLAMMATION, CHRONIC-ACTIVE,- MODERATE, MULTI-FOCAL SEMINAL VESICLES (SV) : -INFLAMMATION, ACUTE,-MODERATELY SEVERE, FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 4323 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 158 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92248 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/26/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 444.3 GRAMS DATE AND TIME OF NECROPSY: 07/26/99 13:55 PROSECTOR: JILL PAUS RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4324 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 159 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92249 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/17/99 STUDY DAY OF DEATH: 499 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/17/99 14:24 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- KIDNEY (KD) : LEFT; VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : >NOTE:>TRIMMING COMMENT: MISSING AT >TISSUE MISSING TIME OF TRIMMING PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 6 X 6 MM, -B-ADENOMA,-NEOPLASM PRESENT RED, DARK RED, TAN AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE >UNREMARKABLE STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -EDEMA,-MODERATELY SEVERE, FOCAL TAN ERODED AREAS, UP TO 3 X 1 MM -EROSION,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC 4325 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 160 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92249 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/17/99 STUDY DAY OF DEATH: 499 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/17/99 14:24 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); LEFT -EROSION,-MODERATE, FOCAL HINDFOOT: SINGLE DARK RED AND TAN -INFLAMMATION, ACUTE,-SLIGHT, FOCAL ABRADED AREA, 10 X 7 MM; COLLECTED -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4326 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 161 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92250 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/02/00 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 872.9 GRAMS DATE AND TIME OF NECROPSY: 01/02/00 13:45 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- KIDNEY (KD) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >BEHAVIOR; HYPOACTIVE; VERIFIED AT SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -RED FOCUS(I)/AREA(S); ALL LOBES: -VACUOLATION, HEPATOCELLULAR,-MODERATE, NECROPSY: OBSERVED MULTIPLE DARK RED AREAS, UP TO 3 MM IN MULTI-FOCAL DIAMETER; COLLECTED ON ROUTINE SECTIONS BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -HYPERTROPHY,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); POSTERIOR ASPECT: 10 X 7 X -HEMORRHAGE,-MODERATELY SEVERE, MULTI- 7 MM, DARK RED AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT EPIDIDYMIDES (EP) : -GRANULOMA, SPERM,-MODERATE, FOCAL 4327 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 162 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92250 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/02/00 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 872.9 GRAMS DATE AND TIME OF NECROPSY: 01/02/00 13:45 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); RIGHT -EDEMA,-MODERATE, FOCAL HINDFOOT: SINGLE TAN AND MOIST ABRADED -EROSION,-MODERATE, FOCAL AREA, 3 MM IN DIAMETER; COLLECTED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -CYST(S); RIGHT, LUMBAR, INVOLVING -DILATATION, SINUSOIDAL,-MODERATELY ENTIRE NODE: SINGLE CLEAR FLUID FILLED SEVERE, MULTI-FOCAL CYST, 18 X 17 X 12 MM (LEFT IS 5 X 4 X 4 MM) (CAP 19) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DARK RED AND DRY; LEFT EYE (RED DISCHARGE) NOT SEEN AT NECROPSY -------------------------------------------- 4328 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 163 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92250 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/02/00 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 872.9 GRAMS DATE AND TIME OF NECROPSY: 01/02/00 13:45 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4329 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 164 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92251 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 750.7 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:45 PROSECTOR: TONY HANFELD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; LUNG (LU) : WEEK 78; 80 X 60MM; VERIFIED AT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NECROPSY: OBSERVED NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT >SKIN & PELAGE; SORE/SCAB; LATERAL- KIDNEY (KD) : KIDNEY (KD) : ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: -DIFFUSELY LIGHT; BILATERAL: TAN -NEPHROPATHY, CHRONIC PROGRESSIVE,- OBSERVED MINIMAL, MULTI-FOCAL >SKIN & PELAGE; YELLOW HAIR COAT; PANCREAS (PA) : VERIFIED AT NECROPSY: NOT OBSERVED -ATROPHY, ACINAR,-SLIGHT, FOCAL >RESPIRATION; IRREGULAR; VERIFIED AT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NECROPSY: NOT APPLICABLE NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT SKIN (SK) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -MISSING/NOT IDENTIFIED; RIGHT: NOT -ONE EXAMINED,-PRESENT POSITIVELY IDENTIFIED; POSSIBLE REMNANTS COLLECTED 4330 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 165 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92251 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 750.7 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:45 PROSECTOR: TONY HANFELD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -ONE EXAMINED,-PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM URINARY BLADDER (UB) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL DEATH COMMENT (DC) : -HISTIOCYTIC SARCOMA,-PRESENT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); RIGHT VENTRAL ABDOMINAL >TISSUE MISSING REGION: SINGLE MASS, 95 X 75 X 46 MM, >NOTE:>MAMMARY MASS DESCRIBED GROSSLY TAN, RED, DARK RED, LOBULATED AND WAS A SUBCUTANEOUS HISTIOCYTIC FIRM; SAME ON CUT SURFACE; COLLECTED; SARCOMA REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN HAS A SINGLE DRY, DARK RED CRUSTED AREA, 38 X 25 MM; COLLECTED WITH MASS; ADHESIONS ASSOCIATED WITH MASS, PLEASE SEE NOTES (MM) 4331 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 166 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92251 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 750.7 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:45 PROSECTOR: TONY HANFELD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, MALE (MM) : >NOTE:>PORTIONS OF MASS ADHERED TO ADIPOSE TISSUE SURROUNDING STOMACH, TESTES AND EPIDIDYMIDES: MULTIPLE TAN FIBROUS ADHESIONS; COLLECTED INTACT WITH MASS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL OBESE GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY -------------------------------------------- 4332 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 167 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92251 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 750.7 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:45 PROSECTOR: TONY HANFELD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) ------------------------------------------------------------------------------------------------------------------------------------ 4333 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 168 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92252 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 683.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:51 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL THYROID (TY) : THYROID (TY) : -LARGE; RIGHT: 7 X 5 X 4 MM (LEFT IS 6 -FIBROSIS,-SLIGHT, MULTI-FOCAL X 4 X 3 MM) -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA, AT >UNREMARKABLE JUNCTION OF GLANDULAR AND NON- GLANDULAR: SINGLE TAN ERODED AREA, 15 X 9 MM; SURROUNDING TISSUE APPEARS THICKENED STOMACH, NONGL (SU) : -EROSION,-MODERATELY SEVERE, FOCAL 4334 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 169 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92252 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 683.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:51 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, NONGL (SU) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL -B-LEIOMYOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4335 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 170 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92252 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 683.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:51 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4336 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 171 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92253 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 753.5 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:25 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0239 .0032 % .0106 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0294 .0039 % .0131 WEIGHT TAKEN BRAIN (BR) 2.2490 .2985 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.2509 .2987 % 1.0008 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.1521 .2856 % .9569 WEIGHT TAKEN LIVER (LI) 17.8062 2.3631 % 7.9174 WEIGHT TAKEN LUNG (LU) 2.0111 .2669 % .8942 WEIGHT TAKEN SPLEEN (SP) 1.1340 .1505 % .5042 WEIGHT TAKEN LEFT TESTIS (TE0) 2.0998 .2787 % .9337 WEIGHT TAKEN RIGHT TESTIS (TE1) 2.1700 .2880 % .9649 WEIGHT TAKEN LF THYROID/PARA (TT0) .0184 .0024 % .0082 WEIGHT TAKEN RT THYROID/PARA (TT1) .0187 .0025 % .0083 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); NEAR MARGIN -LIPIDOSIS, SUBCAPSULAR (TENSION OF MEDIAN LOBES: SINGLE TAN AREA, 4 X LIPIDOSIS),-MODERATELY SEVERE, FOCAL 3 MM (CAP 18) HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 4337 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 172 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92253 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 753.5 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:25 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4338 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 173 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92254 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 443.1 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 13:18 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LUNG (LU) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -FIBROSIS, PLEURAL/SUBPLEURAL,-MINIMAL, >APPEARANCE; THIN; VERIFIED AT FOCAL NECROPSY: OBSERVED -INFILTRATE, MACROPHAGE, ALVEOLAR,- >EYES; RED DISCHARGE; EYES; VERIFIED AT MINIMAL, FOCAL NECROPSY: OBSERVED KIDNEY (KD) : KIDNEY (KD) : >DISCHARGE; NASAL; RED IN COLOR; -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT VERIFIED AT NECROPSY: OBSERVED -ROUGH SURFACE, DIFFUSE; BILATERAL -MINERALIZATION,-MODERATE, FOCAL -DIFFUSELY LIGHT; BILATERAL: TAN -NEPHROPATHY, CHRONIC PROGRESSIVE,- SEVERE, DIFFUSE -THROMBUS,-MODERATELY SEVERE, FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 19 X 12 X 8 -ATROPHY/DEGENERATION,-MODERATE, MULTI- MM; BOTH ARE SOFT FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); LEFT LATERAL -B-FIBROMA,-NEOPLASM PRESENT ABDOMINAL REGION: SINGLE TAN, SLIGHTLY RAISED AREA, 5 MM IN DIAMETER; COLLECTED 4339 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 174 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92254 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 443.1 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 13:18 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -DILATATION, SINUSOIDAL,-SLIGHT, FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -RAISED AREA(S); RIGHT LATERAL ASPECT: -HEMORRHAGE,-MINIMAL, MULTI-FOCAL SINGLE TAN AREA, 1 MM IN DIAMETER -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -LIGHT FOCUS(I)/AREA(S); MUCOSA, NEAR >UNREMARKABLE CARDIAC SPHINCTER: SINGLE TAN, RAISED AREA, 1 MM IN DIAMETER STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; MUCOSA: MULTIPLE TAN -EDEMA,-MODERATE, FOCAL THICKENED AREAS, UP TO 5 X 4 MM -EROSION,-MODERATELY SEVERE, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, FOCAL PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,- URETHRA: TAN AND FIRM MODERATE, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 12 X 8 X 2 -FIBROSIS,-MODERATE, MULTI-FOCAL MM -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL -PIGMENT,-MODERATE, MULTI-FOCAL 4340 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 175 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92254 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 443.1 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 13:18 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT DEATH COMMENT (DC) : -CHRONIC PROGRESSIVE NEPHROPATHY,- PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: -CYST, KERATIN,-PRESENT SINGLE TAN CRUSTED AREA, 9 MM IN -FIBROSIS,-MODERATELY SEVERE, FOCAL DIAMETER; COLLECTED -INFLAMMATION, CHRONIC ACTIVE,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED -DISCHARGE-NASAL; BILATERAL: RED -ANIMAL THIN -------------------------------------------- 4341 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 176 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92254 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 443.1 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 13:18 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4342 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 177 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92255 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 409.0 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:07 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 11.6239 2.8420 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : VERIFIED AT NECROPSY: OBSERVED -NO MACROSCOPIC LESIONS -------------------------------------------- 4343 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 178 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92255 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 409.0 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:07 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4344 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 179 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92256 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 557.9 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:48 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 17.1282 3.0701 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : VERIFIED AT NECROPSY: OBSERVED -NO MACROSCOPIC LESIONS -------------------------------------------- 4345 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 180 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92256 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 557.9 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:48 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4346 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 181 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92257 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 491.1 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:08 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 13.8818 2.8267 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT GENERAL COMMENT (GC) : NECROPSY: NOT APPLICABLE -NO MACROSCOPIC LESIONS -------------------------------------------- 4347 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 182 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92257 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 491.1 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:08 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4348 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 183 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92258 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 549.3 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:13 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 15.7545 2.8681 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : VERIFIED AT NECROPSY: OBSERVED -NO MACROSCOPIC LESIONS -------------------------------------------- 4349 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 184 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92258 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 549.3 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:13 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4350 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 185 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92259 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 343.2 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:34 PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 13.7164 3.9966 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : UNVERIFIED AT NECROPSY -NO MACROSCOPIC LESIONS -------------------------------------------- 4351 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 186 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92259 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 343.2 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:34 PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4352 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 187 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92260 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 501.7 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:19 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 14.0868 2.8078 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : VERIFIED AT NECROPSY: OBSERVED -NO MACROSCOPIC LESIONS -------------------------------------------- 4353 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 188 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92260 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 501.7 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:19 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4354 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 189 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92261 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 775.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:26 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LUNG (LU) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, MULTI-FOCAL LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -DEGENERATION, CYSTIC,-SLIGHT, FOCAL MULTIPLE DARK RED AREAS, UP TO 4 MM IN -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- DIAMETER; COLLECTED ON ROUTINE FOCAL SECTIONS DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4355 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 190 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92261 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 775.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:26 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), TESTIS (TE), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 4356 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 191 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92262 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 458.8 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 9:47 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; DORSAL; LUNG (LU) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >SKIN & PELAGE; SORE/SCAB; HEAD-ENTIRE; FOCAL VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, MACROPHAGE, ALVEOLAR,- >APPEARANCE; THIN; VERIFIED AT SLIGHT, MULTI-FOCAL NECROPSY: OBSERVED KIDNEY (KD) : KIDNEY (KD) : >SKIN & PELAGE; ALOPECIA; VERIFIED AT -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: OBSERVED -DIFFUSELY LIGHT; BILATERAL: TAN MODERATELY SEVERE, DIFFUSE >SKIN & PELAGE; PALE; ENTIRE BODY; -LARGE; BILATERAL: EACH IS 29 X 18 X VERIFIED AT NECROPSY: NOT APPLICABLE 12 MM LIVER (LI) : LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN -DEGENERATION, CYSTIC,-MODERATELY -MASS(ES); RIGHT MEDIAN AND RIGHT SEVERE, FOCAL LATERAL LOBES: SINGLE MASS ON EACH, -HEMATOPOIESIS, EXTRAMEDULLARY,- RIGHT MEDIAN IS 30 X 25 X 20 MM, RIGHT MINIMAL, MULTI-FOCAL LATERAL IS 35 X 20 X 12 MM, BOTH ARE -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- TAN, RED AND VASCULAR, BOTH ARE SAME FOCAL ON CUT SURFACE; RIGHT MEDIAN COLLECTED -INFILTRATE, MAST CELLS,-SLIGHT, MULTI- ON ROUTINE SECTION, RIGHT LATERAL (CAP FOCAL 24); REGIONAL LN (SEE HEPATIC) -B-ADENOMA, HEPATOCELLULAR,- 2 -LARGE; ALL LOBES: MARGINS APPEAR NEOPLASMS PRESENT ROUNDED PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL 4357 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 192 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92262 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 458.8 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 9:47 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PARATHYROID (PT) : PARATHYROID (PT) : -LARGE; BILATERAL: EACH IS 1 MM IN >UNREMARKABLE DIAMETER TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 17 X 8 X 6 -ATROPHY/DEGENERATION,-MODERATELY MM; BOTH ARE SOFT SEVERE, DIFFUSE SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); DORSAL HEAD AND -INFLAMMATION, ACUTE,-MODERATE, MULTI- DORSAL THORACIC REGION: MULTIPLE DARK FOCAL RED, TAN, DRY CRUSTED AREAS, UP TO 5 MM IN DIAMETER (CAP 22) -ALOPECIA-DIFFUSE; (CAP 23) BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT HEART (HT) : HEART (HT) : -LIGHT FOCUS(I)/AREA(S); EPICARDIUM OF -DEGENERATION/NECROSIS,-MINIMAL, MULTI- LEFT VENTRICLE: SINGLE GRAY AREA, 4 X FOCAL 3 MM SPLEEN (SP) : SPLEEN (SP) : -DEPRESSED FOCUS(I)/AREA(S); CRANIAL -HEMATOPOIESIS, EXTRAMEDULLARY, END: MULTIPLE LINEAR DEPRESSED AREAS, INCREASED,-MINIMAL, MULTI-FOCAL UP TO 6 X 1 MM ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 7 MM IN -DEGENERATION, CYSTIC, CORTICAL,- DIAMETER MODERATELY SEVERE, FOCAL -DIFFUSELY LIGHT; BILATERAL: TAN -HEMATOPOIESIS, EXTRAMEDULLARY,- MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MODERATE, FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL 4358 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 193 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92262 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 458.8 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 9:47 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 4 MM IN DIAMETER, DARK RED -HEMORRHAGE,-MODERATE, MULTI-FOCAL AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -MINERALIZATION,-MODERATE, MULTI-FOCAL MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM -THICKENED WALL; DIFFUSE PROSTATE (PR) : PROSTATE (PR) : -SMALL; 15 X 10 X 4 MM -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 10 X 7 X 2 >UNREMARKABLE MM EPIDIDYMIDES (EP) : EPIDIDYMIDES (EP) : -SMALL; BILATERAL: EACH IS 43 X 5 X 4 -HYPOSPERMIA,-PRESENT MM DEATH COMMENT (DC) : -HEPATOCELLULAR ADENOMA,-PRESENT LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; BILATERAL: LEFT IS 4 X 2 X 2 -HEMORRHAGE,-MODERATE, MULTI-FOCAL MM, RIGHT IS 6 X 5 X 2 MM (CAP 26) -DIFFUSELY RED; BILATERAL: DARK RED LN, OTHER (LN) : LN, OTHER (LN) : -LARGE; HEPATIC: UP TO 5 X 3 X 2 MM -INFILTRATE, MACROPHAGE, PIGMENTED,- (CAP 25) MINIMAL, MULTI-FOCAL 4359 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 194 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92262 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 458.8 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 9:47 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), LN, MESENTERIC (MS) ------------------------------------------------------------------------------------------------------------------------------------ 4360 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 195 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92263 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 752.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:11 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); TAIL-PROXIMAL; KIDNEY (KD) : WEEK 64; VERIFIED AT NECROPSY: -CYST,-SLIGHT, FOCAL OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EXCRETION; NONFORMED FECES; VERIFIED MODERATELY SEVERE, DIFFUSE AT NECROPSY: NOT OBSERVED LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : PANCREAS (PA) : -MASS(ES); DUODENUM REGION: SINGLE -ATROPHY, ACINAR,-MODERATE, MULTI-FOCAL MASS, 15 X 10 X 10 MM, RED, DARK RED, -FIBROSIS,-SLIGHT, MULTI-FOCAL TAN, AND FIRM; SAME ON CUT SURFACE -B-ADENOMA, ISLET CELL,-NEOPLASM (CAP 22); REGIONAL LN (PANCREATIC) NOT PRESENT IDENTIFIED THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT SPLEEN (SP) : SPLEEN (SP) : -RAISED FOCUS(I)/AREA(S); PARIETAL -B-LEIOMYOMA,-NEOPLASM PRESENT SURFACE, ALONG MARGIN: SINGLE RED AREA, 7 X 5 X 4 MM 4361 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 196 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92263 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 752.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:11 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT TAIL (TI) : TAIL (TI) : -RAISED AREA(S); NEAR BASE: SINGLE -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM DRY, TAN RAISED AREA, 8 X 2 X 1 MM; PRESENT COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), TESTIS (TE), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 4362 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 197 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92264 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 313.7 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:34 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 11.7274 3.7384 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : UNVERIFIED AT NECROPSY -NO MACROSCOPIC LESIONS -------------------------------------------- 4363 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 198 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92264 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 313.7 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:34 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) ------------------------------------------------------------------------------------------------------------------------------------ 4364 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 199 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92265 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/16/99 STUDY DAY OF DEATH: 559 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/16/99 8:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : LUNG (LU) : -UNCOLLAPSED; ALL LOBES >UNREMARKABLE KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- -DIFFUSELY LIGHT; BILATERAL: TAN SEVERE, DIFFUSE -LARGE; BILATERAL: EACH IS 30 X 25 X 21 MM LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); VISCERAL >UNREMARKABLE SURFACE OF RIGHT MEDIAN LOBE: MULTIPLE DARK RED AREAS, UP TO 4 X 3 MM; COLLECTED ON ROUTINE SECTION PANCREAS (PA) : PANCREAS (PA) : -LARGE VESSELS; DIFFUSE, ALONG -THROMBUS,-SEVERE, MULTI-FOCAL DUODENUM: UP TO 4 MM IN DIAMETER (CAP 21) PARATHYROID (PT) : PARATHYROID (PT) : -LARGE; LEFT: 3 X 2 X 1 MM (RIGHT IS 1 -HYPERPLASIA,-MODERATELY SEVERE, MM IN DIAMETER) DIFFUSE EYE (EY) : EYE (EY) : -OPAQUE CORNEA; RIGHT >UNREMARKABLE PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MODERATE, FOCAL 4365 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 200 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92265 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/16/99 STUDY DAY OF DEATH: 559 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/16/99 8:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); TISSUE -B-ADENOMA,-NEOPLASM PRESENT SURROUNDING MID ASPECT: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EROSION,-MINIMAL, FOCAL MULTIPLE BLACK FOCI, UP TO 4 MM IN DIAMETER PROSTATE (PR) : PROSTATE (PR) : -SMALL; 10 X 8 X 7 MM -INFLAMMATION, ACUTE,-MINIMAL, MULTI- FOCAL DEATH COMMENT (DC) : -CHRONIC PROGRESSIVE NEPHROPATHY,- PRESENT AORTA (AO) : AORTA (AO) : -LARGE; DIFFUSE: UP TO 6 MM IN -MINERALIZATION,-MINIMAL, MULTI-FOCAL DIAMETER -FIRM; DIFFUSE LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; BILATERAL: LEFT IS 6 X 5 X 5 -DILATATION, SINUSOIDAL,-MODERATE, MM, RIGHT IS 4 X 4 X 3 MM MULTI-FOCAL -DIFFUSELY RED; BILATERAL: DARK RED -HEMORRHAGE,-SLIGHT, MULTI-FOCAL (CAP 19) LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -DIFFUSELY DARK; BILATERAL: BLACK (CAP >UNREMARKABLE 20) GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, LOWER INCISORS 4366 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 201 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92265 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/16/99 STUDY DAY OF DEATH: 559 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/16/99 8:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -DISCHARGE-NASAL; DRY, RED MATERIAL -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN OFF AT BASE -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: THYROID (TY), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 4367 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 202 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92266 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 805.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:54 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); DORSAL; WEEK 76; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >WART LIKE LESION(S); VENTRAL- FOCAL ABDOMINAL; WEEK 92; VERIFIED AT -MINERALIZATION, PELVIC,-MINIMAL, MULTI- NECROPSY: OBSERVED FOCAL LIVER (LI) : LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); LEFT LATERAL -FOCUS, ALTERED HEPATOCELLULAR, LOBE: SINGLE TAN AREA, 2 MM IN EOSINOPHILIC,-MODERATE, FOCAL DIAMETER; COLLECTED ON ROUTINE SECTION -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); MID DORSAL LUMBAR -CYST, EPIDERMAL INCLUSION,-PRESENT REGION: SINGLE DARK RED AND DRY AREA, -EROSION,-SEVERE, FOCAL 21 X 22 MM; COLLECTED -INFLAMMATION, ACUTE,-MODERATELY -RAISED AREA(S); MID ABDOMINAL REGION: SEVERE, FOCAL SINGLE TAN AND RED AREA, 6 X 5 X 5 MM -B-FIBROMA,-NEOPLASM PRESENT (CAP 22) URINARY BLADDER (UB) : -CALCULUS,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4368 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 203 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92266 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 805.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:54 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4369 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 204 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92267 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 611.7 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:16 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0250 .0041 % .0104 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0313 .0051 % .0130 WEIGHT TAKEN BRAIN (BR) 2.4148 .3948 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.1305 .3483 % .8823 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.1979 .3593 % .9102 WEIGHT TAKEN LIVER (LI) 16.2986 2.6645 % 6.7495 WEIGHT TAKEN LUNG (LU) 2.0076 .3282 % .8314 WEIGHT TAKEN SPLEEN (SP) 1.0302 .1684 % .4266 WEIGHT TAKEN LEFT TESTIS (TE0) 1.9753 .3229 % .8180 WEIGHT TAKEN RIGHT TESTIS (TE1) 2.0027 .3274 % .8293 WEIGHT TAKEN LF THYROID/PARA (TT0) .0232 .0038 % .0096 WEIGHT TAKEN RT THYROID/PARA (TT1) .0296 .0048 % .0123 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; DEATH COMMENT (DC) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 4370 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 205 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92267 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 611.7 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:16 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4371 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 206 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92268 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 675.4 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 13:05 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; SCROTUM- KIDNEY (KD) : KIDNEY (KD) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- >APPEARANCE; THIN; VERIFIED AT -GRANULAR MATERIAL; BILATERAL, WITHIN MODERATE, MULTI-FOCAL NECROPSY: NOT OBSERVED PELVIS: MULTIPLE TAN PINPOINT GRANULES LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATELY SEVERE, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); OVERLYING RIGHT -EDEMA,-MODERATE, FOCAL SCROTAL SAC: SINGLE TAN CRUSTED AREA, -EROSION,-MODERATE, FOCAL 6 MM IN DIAMETER; COLLECTED (CAP 19) -INFLAMMATION, ACUTE,-SLIGHT, FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 X 5 X 5 MM (RIGHT IS 5 -HEMORRHAGE,-MINIMAL, MULTI-FOCAL X 3 X 3 MM) PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 5 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND FIRM STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: TWO >UNREMARKABLE BROWN AREAS, UP TO 4 X 2 MM 4372 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 207 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92268 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 675.4 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 13:05 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ URINARY BLADDER (UB) : URINARY BLADDER (UB) : -DIFFUSELY RED; MUCOSA -HYPERPLASIA, TRANSITIONAL CELL,- MODERATE, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4373 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 208 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92269 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/24/99 STUDY DAY OF DEATH: 506 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/24/99 11:14 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE >UNREMARKABLE DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- 4374 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 209 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92269 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/24/99 STUDY DAY OF DEATH: 506 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/24/99 11:14 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4375 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 210 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92270 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 757.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:06 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LUNG (LU) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MODERATE, FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 20 X 14 X 5 -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE MM; BOTH APPEAR SOFT -MINERALIZATION,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: -HEMORRHAGE,-MINIMAL, MULTI-FOCAL SINGLE DARK RED AREA, 4 X 2 MM -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4376 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 211 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92270 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 757.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:06 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 4377 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 212 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92271 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 625 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/21/99 8:32 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 90; 10 X 10MM; LUNG (LU) : LUNG (LU) : VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO MULTIPLE TAN AREAS, UP TO 5 MM IN NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT DIAMETER -ADHESION(S); ALL LOBES TO DIAPHRAGM: MULTIPLE TAN FIBROUS ADHESIONS; COLLECTED INTACT KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, DIFFUSE LIVER (LI) : LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO MULTIPLE TAN AREAS, UP TO 6 MM IN NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT DIAMETER; COLLECTED ON ROUTINE SECTIONS PANCREAS (PA) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYROID (TY) : THYROID (TY) : -LARGE; BILATERAL: EACH IS 10 X 5 X 5 >UNREMARKABLE MM SKIN (SK) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 4378 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 213 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92271 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 625 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/21/99 8:32 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SPLEEN (SP) : SPLEEN (SP) : -LIGHT FOCUS(I)/AREA(S); PARIETAL >UNREMARKABLE SURFACE: SINGLE YELLOW AREA, 4 X 3 MM ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 10 X 8 X 8 MM (RIGHT IS -VACUOLATION,-MODERATE, FOCAL 7 X 6 X 6 MM) ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO MULTIPLE BROWN AREAS, UP TO 4 X 3 MM NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -ADHESION(S); SEROSA ADHERED TO LEFT LATERAL LOBE OF LIVER: MULTIPLE TAN FIBROUS ADHESIONS; COLLECTED INTACT WITH STOMACH DEATH COMMENT (DC) : -HISTIOCYTIC SARCOMA,-PRESENT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); DORSAL LUMBAR REGION: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO SINGLE MASS, 20 X 15 X 10 MM, TAN AND NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST 4379 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 214 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92271 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 625 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/21/99 8:32 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -URINARY BLADDER-LARGE LUMEN; 30 X 25 X 20 MM -DISCHARGE-OCULAR; BILATERAL: DARK RED AND MOIST -DISCHARGE-NASAL; DARK RED AND MOIST -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: PANCREAS (PA), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) ------------------------------------------------------------------------------------------------------------------------------------ 4380 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 215 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92272 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/16/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 671.7 GRAMS DATE AND TIME OF NECROPSY: 11/16/99 10:10 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); HEAD-MAXILLARY-RIGHT; WEEK LUNG (LU) : 74; 30 X 40MM; VERIFIED AT NECROPSY: -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- OBSERVED FOCAL >SKIN & PELAGE; SORE/SCAB; HEAD- -MINERALIZATION, VASCULAR,-MINIMAL, MAXILLARY-RIGHT; VERIFIED AT NECROPSY: FOCAL OBSERVED KIDNEY (KD) : >SKIN & PELAGE; SORE/SCAB; PAW-HIND- -NEPHROPATHY, CHRONIC PROGRESSIVE,- RIGHT; VERIFIED AT NECROPSY: OBSERVED MINIMAL, MULTI-FOCAL LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: >UNREMARKABLE MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS SKIN (SK) : -EROSION,-SLIGHT, MULTI-FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL -M-CARCINOMA, SQUAMOUS CELL,-NEOPLASM PRESENT MUSCLE, SKELETAL (SM) : -I-SQUAMOUS CELL CARCINOMA,-NEOPLASM PRESENT 4381 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 216 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92272 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/16/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 671.7 GRAMS DATE AND TIME OF NECROPSY: 11/16/99 10:10 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MUSCLE, SKELETAL (SM) : -MASS(ES); ATTACHED TO RIGHT MANDIBLE, SURROUNDING RIGHT PINNA: SINGLE MASS, 40 X 25 X 20 MM, RED, DARK RED, DRY, AND CRUSTED; ON SECTION, EXUDES A GREEN AND TAN CASEOUS MATERIAL; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 19); OVERLYING SKIN OF MASS HAS A SINGLE DARK RED CRUSTED AREA, 20 X 15 X 7 MM; COLLECTED INTACT WITH MASS DEATH COMMENT (DC) : -SQUAMOUS CELL CARCINOMA,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, FOCAL -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MULTI-FOCAL FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); RIGHT -EDEMA,-MODERATE, MULTI-FOCAL HINDFOOT: SINGLE DARK RED, MOIST -EROSION,-MODERATELY SEVERE, MULTI- ABRADED AREA, 7 X 6 MM; COLLECTED FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 4382 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 217 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92272 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/16/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 671.7 GRAMS DATE AND TIME OF NECROPSY: 11/16/99 10:10 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), TESTIS (TE), MAMMARY, MALE (MM) ------------------------------------------------------------------------------------------------------------------------------------ 4383 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 218 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92273 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/24/99 STUDY DAY OF DEATH: 294 STUDY WEEK OF DEATH: 42 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/24/99 13:23 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL CECUM (CE) : CECUM (CE) : -THICKENED WALL; MID CECUM >UNREMARKABLE DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- 4384 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 219 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92273 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/24/99 STUDY DAY OF DEATH: 294 STUDY WEEK OF DEATH: 42 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/24/99 13:23 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4385 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 220 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92274 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 655.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:02 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0376 .0057 % .0153 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0272 .0041 % .0111 WEIGHT TAKEN BRAIN (BR) 2.4537 .3741 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.2200 .3385 % .9048 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.2397 .3415 % .9128 WEIGHT TAKEN LIVER (LI) 21.2986 3.2472 % 8.6802 WEIGHT TAKEN LUNG (LU) 2.0321 .3098 % .8282 WEIGHT TAKEN SPLEEN (SP) 1.1591 .1767 % .4724 WEIGHT TAKEN LEFT TESTIS (TE0) 2.2539 .3436 % .9186 WEIGHT TAKEN RIGHT TESTIS (TE1) 2.2901 .3492 % .9333 WEIGHT TAKEN LF THYROID/PARA (TT0) .0236 .0036 % .0096 WEIGHT TAKEN RT THYROID/PARA (TT1) .0289 .0044 % .0118 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE -BONE MARROW SMEAR TAKEN -------------------------------------------- 4386 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 221 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92274 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 655.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:02 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4387 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 222 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92275 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 417.5 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 15:29 PROSECTOR: JERALD W. JOHNSON RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT LUNG (LU) : LUNG (LU) : NECROPSY: NOT OBSERVED -ADHESION(S); ALL LOBES TO EACH OTHER -FIBROSIS, PLEURAL/SUBPLEURAL,- >SKIN & PELAGE; PALE; ENTIRE BODY; AND PLEURA: MULTIPLE GREY AND FIBROUS MODERATE, MULTI-FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE ADHESIONS; COLLECTED INTACT >BEHAVIOR; HYPOACTIVE; VERIFIED AT -DIFFUSELY RED; ALL LOBES: DARK RED NECROPSY: NOT APPLICABLE KIDNEY (KD) : KIDNEY (KD) : >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -ROUGH SURFACE, DIFFUSE; BILATERAL -CYST,-MODERATE, MULTI-FOCAL AT NECROPSY: NOT APPLICABLE -CYST(S); BILATERAL, CORTEX EXTENDING -NEPHROPATHY, CHRONIC PROGRESSIVE,- INTO MEDULLA: MULTIPLE CLEAR FLUID MODERATELY SEVERE, DIFFUSE FILLED CYSTS, UP TO 5 MM IN DIAMETER LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : PANCREAS (PA) : -LARGE VESSELS; DIFFUSE: UP TO 3 MM IN -ATROPHY, ACINAR,-SLIGHT, FOCAL DIAMETER (CAP 22) -HYPERTROPHY, VASCULAR,-MODERATELY SEVERE, MULTI-FOCAL -INFLAMMATION, VASCULAR,-SLIGHT, MULTI- FOCAL PARATHYROID (PT) : PARATHYROID (PT) : -LARGE; BILATERAL: EACH IS 1 MM IN -HYPERPLASIA,-SLIGHT, DIFFUSE DIAMETER 4388 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 223 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92275 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 417.5 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 15:29 PROSECTOR: JERALD W. JOHNSON RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -LARGE; PROXIMAL TO CECUM: UP TO 9 X 5 >UNREMARKABLE X 5 MM (CAP 24) SPLEEN (SP) : SPLEEN (SP) : -MASS(ES); CRANIAL END: SINGLE MASS, 9 -THROMBUS,-MODERATELY SEVERE, FOCAL MM IN DIAMETER, RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (PANCREATIC) NOT IDENTIFIED PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 5 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL TO URETHRA: TAN, -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, GREEN AND FIRM MULTI-FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -LARGE; ANTERIOR MESENTERIC: UP TO 5 X -DILATATION, SINUSOIDAL,-MODERATE, 3 X 2 MM (CAP 23) MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT, MULTI-FOCAL 4389 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 224 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92275 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 417.5 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 15:29 PROSECTOR: JERALD W. JOHNSON RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LIVER (LI), THYROID (TY), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 4390 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 225 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92276 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/28/99 STUDY DAY OF DEATH: 510 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/28/99 7:53 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT LUNG (LU) : LUNG (LU) : NECROPSY: OBSERVED -UNCOLLAPSED; ALL LOBES -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >SKIN & PELAGE; RED HAIR COAT; DORSAL- FOCAL CERVICAL-RIGHT; VERIFIED AT NECROPSY: KIDNEY (KD) : KIDNEY (KD) : NOT OBSERVED -ROUGH SURFACE, DIFFUSE; BILATERAL -MINERALIZATION,-SLIGHT, FOCAL -DIFFUSELY LIGHT; BILATERAL: TAN -NEPHROPATHY, CHRONIC PROGRESSIVE,- SEVERE, DIFFUSE TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATELY SEVERE, DIFFUSE DEATH COMMENT (DC) : -CHRONIC PROGRESSIVE NEPHROPATHY,- PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY BROWN MATERIAL -ANIMAL THIN -DISCHARGE-ORAL; DRY BROWN MATERIAL -------------------------------------------- 4391 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 226 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92276 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/28/99 STUDY DAY OF DEATH: 510 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/28/99 7:53 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 4392 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 227 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92277 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/25/99 STUDY DAY OF DEATH: 415 STUDY WEEK OF DEATH: 60 TERMINAL BODY WEIGHT: 595.0 GRAMS DATE AND TIME OF NECROPSY: 05/25/99 13:14 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NO FECES; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >SKIN & PELAGE; BROWN HAIR COAT; MODERATE, MULTI-FOCAL VENTRAL-ABDOMINAL; VERIFIED AT PANCREAS (PA) : NECROPSY: NOT OBSERVED -PIGMENT,-MINIMAL, MULTI-FOCAL >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED THYROID (TY) : AT NECROPSY: NOT APPLICABLE -HYPERPLASIA, C-CELL,-MODERATE, FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, GREY, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE GREY AREAS, UP TO 4 X 3 MM STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA, NEAR -EDEMA,-MODERATELY SEVERE, DIFFUSE CARDIAC VALVE: SINGLE DARK RED ERODED -EROSION,-MODERATELY SEVERE, FOCAL AREA, 5 X 4 X 3 MM; SURROUNDING TISSUE -HEMORRHAGE,-SLIGHT, MULTI-FOCAL APPEARS SWOLLEN -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4393 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 228 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92277 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/25/99 STUDY DAY OF DEATH: 415 STUDY WEEK OF DEATH: 60 TERMINAL BODY WEIGHT: 595.0 GRAMS DATE AND TIME OF NECROPSY: 05/25/99 13:14 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI) ------------------------------------------------------------------------------------------------------------------------------------ 4394 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 229 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92278 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 793.9 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:03 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NONFORMED FECES; VERIFIED LUNG (LU) : AT NECROPSY: NOT APPLICABLE -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -FOCUS, ALTERED HEPATOCELLULAR, MULTIPLE DARK RED AREAS, UP TO 3 MM IN EOSINOPHILIC,-MODERATE, FOCAL DIAMETER; COLLECTED ON ROUTINE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- SECTIONS FOCAL LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY RED; DARK RED -HEMORRHAGE,-MODERATE, MULTI-FOCAL -LARGE; UP TO 7 MM IN DIAMETER -N-HEMANGIOSARCOMA,-NEOPLASM PRESENT SPLEEN (SP) : SPLEEN (SP) : -RAISED FOCUS(I)/AREA(S); PARIETAL -M-HEMANGIOSARCOMA,-NEOPLASM PRESENT SURFACE: SINGLE DARK RED, TAN RAISED AREA, 8 X 7 X 7 MM PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); MID ASPECT: -CYST,-PRESENT SINGLE RED AREA, 3 X 2 MM -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4395 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 230 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92278 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 793.9 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:03 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4396 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 231 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92279 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 539.1 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 16:00 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: NOT APPLICABLE -LARGE PELVIS(ES); LEFT -DILATATION, PELVIC,-PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT -MINERALIZATION, PELVIC,-MODERATE, NECROPSY: NOT APPLICABLE FOCAL >APPEARANCE; HEAD TILT; RIGHT; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; HUNCHED; VERIFIED AT PITUITARY (PI) : PITUITARY (PI) : NECROPSY: NOT APPLICABLE -MASS(ES); 11 X 10 X 8 MM, DARK RED, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL GREY AND SOFT -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, URETHRA: GREEN AND FIRM MULTI-FOCAL EPIDIDYMIDES (EP) : EPIDIDYMIDES (EP) : -MASS(ES); ATTACHED TO CAUDAL END OF -GRANULOMA, SPERM,-SEVERE, FOCAL RIGHT: SINGLE GREEN AND SOFT MASS, 8 X -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- 5 X 5 MM; ON SECTION EXUDES A TAN FOCAL CASEOUS MATERIAL; COLLECTED INTACT; REGIONAL LN (SEE LUMBAR) DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; RIGHT: 6 X 5 X 2 MM (LEFT IS 5 -DILATATION, SINUSOIDAL,-SLIGHT, MULTI- X 4 X 2 MM) (CAP 19) FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 4397 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 232 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92279 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 539.1 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 16:00 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), SKIN (SK), MAMMARY, MALE (MM) ------------------------------------------------------------------------------------------------------------------------------------ 4398 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 233 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92280 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 306.1 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:29 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 12.3496 4.0345 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : UNVERIFIED AT NECROPSY -NO MACROSCOPIC LESIONS -------------------------------------------- 4399 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 234 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92280 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 306.1 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:29 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) ------------------------------------------------------------------------------------------------------------------------------------ 4400 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 235 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92281 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 671.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:39 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LUNG (LU) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -INFARCT,-MODERATE, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE, MULTI-FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SINGLE DARK RED FOCUS, 3 MM IN -B-ADENOMA,-NEOPLASM PRESENT DIAMETER STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE >UNREMARKABLE BROWN AREA, LESS THAN 1 MM IN DIAMETER URINARY BLADDER (UB) : -CALCULUS,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4401 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 236 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92281 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 671.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:39 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4402 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 237 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92282 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 568.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 12:39 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; YELLOW HAIR COAT; KIDNEY (KD) : VERIFIED AT NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EXCRETION; NONFORMED FECES; VERIFIED MINIMAL, MULTI-FOCAL AT NECROPSY: NOT APPLICABLE SKIN (SK) : SKIN (SK) : >WART LIKE LESION(S); LIMB-FRONT-RIGHT; -CRUSTED AREA(S); RIGHT FRONT -B-ADENOMA, BASAL CELL,-NEOPLASM WEEK 105; VERIFIED AT NECROPSY: SHOULDER: SINGLE TAN RAISED AREA, 4 X PRESENT OBSERVED 3 X 2 MM (CAP 22) LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY RED >UNREMARKABLE STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE -INFLAMMATION, CHRONIC-ACTIVE,- TAN ERODED AREA, 3 MM IN DIAMETER; MODERATE, MULTI-FOCAL SURROUNDING TISSUE APPEARS SWOLLEN -M-CARCINOMA, SQUAMOUS CELL,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4403 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 238 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92282 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 568.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 12:39 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4404 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 239 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92283 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 683.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:04 PROSECTOR: TONY HANFELD RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); TAIL-MID; WEEK KIDNEY (KD) : 101; VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, FOCAL -INFARCT,-MINIMAL, FOCAL LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: >UNREMARKABLE MULTIPLE DARK RED AREAS, UP TO 4 X 3 MM; COLLECTED ON ROUTINE SECTIONS PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE >UNREMARKABLE URINARY BLADDER (UB) : -CALCULUS,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT TAIL (TI) : TAIL (TI) : -RAISED AREA(S); 80 MM FROM TIP: -CYST, EPIDERMAL INCLUSION,-PRESENT SINGLE TAN RAISED AREA, 4 X 3 X 2 MM; COLLECTED LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -LARGE; UP TO 6 X 4 X 4 MM (CAP 22) >TISSUE MISSING >NOTE:>PANCREATIC LYMPH NODE DESCRIBED GROSSLY WAS A ISLET CELL ADENOMA OF THE PANCREAS 4405 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 240 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92283 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 683.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:04 PROSECTOR: TONY HANFELD RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4406 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 241 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92284 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 320.0 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 11.1979 3.4993 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : UNVERIFIED AT NECROPSY -NO MACROSCOPIC LESIONS -------------------------------------------- 4407 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 242 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92284 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 320.0 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) ------------------------------------------------------------------------------------------------------------------------------------ 4408 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 243 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92285 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 527 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 671.2 GRAMS DATE AND TIME OF NECROPSY: 09/14/99 11:53 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; ROUGH HAIR COAT; KIDNEY (KD) : VERIFIED AT NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >BEHAVIOR; HYPOACTIVE; VERIFIED AT MODERATELY SEVERE, MULTI-FOCAL NECROPSY: NOT APPLICABLE BRAIN (BR) : BRAIN (BR) : >EXCRETION; FEW FECES; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE -DILATATION, VENTRICULAR,-PRESENT HEART (HT) : HEART (HT) : -LIGHT FOCUS(I)/AREA(S); EPICARDIUM OF >UNREMARKABLE BOTH VENTRICLES: MULTIPLE INDISTINCT GREY AREAS PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 MM IN DIAMETER, RED, DARK -HEMORRHAGE,-SLIGHT, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : PROSTATE (PR) : -LARGE; 40 X 25 X 10 MM -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; RIGHT: 38 X 20 X 8 MM (LEFT IS -SECRETION, INCREASED,-PRESENT 25 X 15 X 4 MM) DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; LEFT: 13 X 6 X 5 MM (RIGHT IS -DILATATION, SINUSOIDAL,-MODERATELY 5 X 3 X 2 MM); BOTH APPEAR CYSTIC (CAP SEVERE, MULTI-FOCAL 19) 4409 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 244 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92285 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 527 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 671.2 GRAMS DATE AND TIME OF NECROPSY: 09/14/99 11:53 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4410 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 245 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92286 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 771.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:02 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0266 .0035 % .0117 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0234 .0030 % .0103 WEIGHT TAKEN BRAIN (BR) 2.2692 .2943 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.5875 .3356 % 1.1403 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.5622 .3323 % 1.1291 WEIGHT TAKEN LIVER (LI) 21.6521 2.8083 % 9.5417 WEIGHT TAKEN LUNG (LU) 1.8660 .2420 % .8223 WEIGHT TAKEN SPLEEN (SP) 1.0195 .1322 % .4493 WEIGHT TAKEN LEFT TESTIS (TE0) .7475 .0970 % .3294 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.9291 .2502 % .8501 WEIGHT TAKEN LF THYROID/PARA (TT0) .0175 .0023 % .0077 WEIGHT TAKEN RT THYROID/PARA (TT1) .0214 .0028 % .0094 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -LARGE PELVIS(ES); BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, MULTI- FOCAL 4411 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 246 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92286 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 771.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:02 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TESTIS (TE) : TESTIS (TE) : -SMALL; LEFT: 21 X 10 X 4 MM (FOR -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE COMPARISON RIGHT IS 26 X 12 X 7 MM) >NOTE:>LEFT TESTIS DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA) ------------------------------------------------------------------------------------------------------------------------------------ 4412 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 247 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92287 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 719.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:16 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0348 .0048 % .0151 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0259 .0036 % .0113 WEIGHT TAKEN BRAIN (BR) 2.2991 .3194 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.9967 .2774 % .8685 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.9629 .2727 % .8538 WEIGHT TAKEN LIVER (LI) 22.1931 3.0828 % 9.6530 WEIGHT TAKEN LUNG (LU) 2.0899 .2903 % .9090 WEIGHT TAKEN SPLEEN (SP) 1.0159 .1411 % .4419 WEIGHT TAKEN LEFT TESTIS (TE0) 1.5601 .2167 % .6786 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.5601 .2167 % .6786 WEIGHT TAKEN LF THYROID/PARA (TT0) .0259 .0036 % .0113 WEIGHT TAKEN RT THYROID/PARA (TT1) .0265 .0037 % .0115 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LUNG (LU) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE 4413 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 248 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92287 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 719.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:16 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4414 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 249 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92288 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 853.2 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:03 PROSECTOR: MONTI THOMAS MAGUIRE RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0402 .0047 % .0164 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0302 .0035 % .0123 WEIGHT TAKEN BRAIN (BR) 2.4494 .2871 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.1246 .2490 % .8674 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.2139 .2595 % .9039 WEIGHT TAKEN LIVER (LI) 20.9207 2.4520 % 8.5412 WEIGHT TAKEN LUNG (LU) 1.6636 .1950 % .6792 WEIGHT TAKEN SPLEEN (SP) 1.0951 .1284 % .4471 WEIGHT TAKEN LEFT TESTIS (TE0) 1.7259 .2023 % .7046 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.8021 .2112 % .7357 WEIGHT TAKEN LF THYROID/PARA (TT0) .0253 .0030 % .0103 WEIGHT TAKEN RT THYROID/PARA (TT1) .0203 .0024 % .0083 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE -BONE MARROW SMEAR TAKEN -------------------------------------------- 4415 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 250 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92288 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 853.2 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:03 PROSECTOR: MONTI THOMAS MAGUIRE RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4416 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 251 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92289 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 525.8 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:41 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; BROWN HAIR COAT; KIDNEY (KD) : KIDNEY (KD) : PERINEAL AREA; VERIFIED AT NECROPSY: -LARGE PELVIS(ES); BILATERAL -CYST,-SLIGHT, MULTI-FOCAL NOT OBSERVED -ROUGH SURFACE, DIFFUSE; BILATERAL -DILATATION, PELVIC,-PRESENT >APPEARANCE; THIN; VERIFIED AT -DIFFUSELY LIGHT; BILATERAL: TAN -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: OBSERVED -LARGE; BILATERAL: EACH IS 14 X 18 X SEVERE, DIFFUSE 28 MM LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL TESTIS (TE) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; BILATERAL: LEFT IS 2 X 5 X 14 -HEMORRHAGE,-MODERATE, MULTI-FOCAL MM, RIGHT IS 3 X 4 X 10 MM (CAP 22) -INFILTRATE, MACROPHAGE, VACUOLATED,- -MOTTLED; BILATERAL: RED, DARK RED AND SLIGHT, MULTI-FOCAL TAN LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; BILATERAL: LEFT IS 5 X 5 X 8 -DILATATION, SINUSOIDAL,-MODERATELY MM, RIGHT IS 6 X 7 X 14 MM (CAP 23) SEVERE, MULTI-FOCAL -DIFFUSELY RED; BILATERAL: DARK RED -FIBROSIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 4417 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 252 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92289 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 525.8 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:41 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; BROWN -ANIMAL THIN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 4418 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 253 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92290 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 438 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 360.2 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 14:57 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NO FECES; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >APPEARANCE; THIN; VERIFIED AT FOCAL NECROPSY: OBSERVED BRAIN (BR) : BRAIN (BR) : >APPEARANCE; HUNCHED; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE PITUITARY (PI) : PITUITARY (PI) : >SKIN & PELAGE; ROUGH HAIR COAT; -MASS(ES); 12 X 9 X 7 MM, RED, DARK -HEMORRHAGE,-SLIGHT, MULTI-FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE RED, TAN, LOBULATED AND FIRM -B-ADENOMA,-NEOPLASM PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT DEATH COMMENT (DC) : NECROPSY: NOT APPLICABLE -PITUITARY ADENOMA,-PRESENT >RESPIRATION; IRREGULAR; VERIFIED AT GENERAL COMMENT (GC) : NECROPSY: NOT APPLICABLE -BLOOD FILM TAKEN >BEHAVIOR; ATAXIC; VERIFIED AT -ANIMAL THIN NECROPSY: NOT APPLICABLE -------------------------------------------- 4419 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 254 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92290 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 438 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 360.2 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 14:57 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4420 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 255 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92291 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/07/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/07/99 8:20 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LUNG (LU) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE, DIFFUSE LIVER (LI) : LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC -FOCUS, ALTERED HEPATOCELLULAR, SURFACE OF LEFT LATERAL LOBE: SINGLE EOSINOPHILIC,-MODERATE, FOCAL TAN AREA, 4 X 1 MM; COLLECTED ON -HEMORRHAGE,-SLIGHT, MULTI-FOCAL ROUTINE SECTION -INFLAMMATION, CHRONIC,-MODERATE, FOCAL -NECROSIS, HEPATOCELLULAR,-MODERATE, FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 18 MM IN DIAMETER (RIGHT >UNREMARKABLE IS 3 MM IN DIAMETER) >NOTE:>ADRENAL CORTICAL LESIONS DESCRIBED GROSSLY WERE A PHEOCHROMOCYTOMA OF THE MEDULLA ADRENAL, MEDULLA (MA) : -HEMATOPOIESIS, EXTRAMEDULLARY,- MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 4421 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 256 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92291 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/07/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/07/99 8:20 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM DEATH COMMENT (DC) : -PHEOCHROMOCYTOMA,-PRESENT BILE DUCT (BD) : BILE DUCT (BD) : -LARGE; UP TO 3 MM IN DIAMETER (CAP -DILATATION,-SEVERE, FOCAL 19) -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4422 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 257 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92292 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 463 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 373.4 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:22 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; SORE/SCAB; PAW-HIND- PITUITARY (PI) : PITUITARY (PI) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -MASS(ES); 12 X 9 X 9 MM, RED, DARK -HEMORRHAGE,-MODERATELY SEVERE, MULTI- >APPEARANCE; HUNCHED; VERIFIED AT RED, TAN, GRAY, AND SOFT FOCAL NECROPSY: NOT APPLICABLE -B-ADENOMA,-NEOPLASM PRESENT >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : AT NECROPSY: NOT APPLICABLE -THICKENED WALL; DIFFUSE >UNREMARKABLE >RESPIRATION; IRREGULAR; VERIFIED AT DEATH COMMENT (DC) : NECROPSY: NOT APPLICABLE -PITUITARY ADENOMA,-PRESENT >EYES; SQUINTED; PERI-ORBITAL; VERIFIED FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : AT NECROPSY: NOT OBSERVED -ABRASION(S)/ULCERATION(S); RIGHT HIND -FIBROSIS,-MODERATE, FOCAL >EXCRETION; FEW FECES; VERIFIED AT FOOT: SINGLE TAN, MOIST, AND ABRADED -INFLAMMATION, ACUTE,-MINIMAL, FOCAL NECROPSY: NOT APPLICABLE AREA, 4 MM IN DIAMETER; COLLECTED TAIL (TI) : TAIL (TI) : -CRUSTED AREA(S); PROXIMAL ONE-THIRD -INFLAMMATION, ACUTE,-MODERATE, MULTI- OF TAIL: MULTIPLE, TAN, AND DRY FOCAL CRUSTED AREAS, UP TO 2 X 1 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 4423 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 258 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92292 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 463 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 373.4 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:22 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4424 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 259 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92293 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/18/99 STUDY DAY OF DEATH: 288 STUDY WEEK OF DEATH: 42 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/18/99 12:52 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT -ROUGH SURFACE, DIFFUSE; BILATERAL -MINERALIZATION, TUBULAR,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL LIVER (LI) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT URINARY BLADDER (UB) : URINARY BLADDER (UB) : -DIFFUSELY RED; DIFFUSE >UNREMARKABLE SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: YELLOW -SECRETION, INCREASED,-PRESENT DEATH COMMENT (DC) : -MALIGNANT LYMPHOMA,-PRESENT HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT GENERAL COMMENT (GC) : -URINARY BLADDER-LARGE LUMEN; 35 X 25 X 20 MM -------------------------------------------- 4425 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 260 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92293 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/18/99 STUDY DAY OF DEATH: 288 STUDY WEEK OF DEATH: 42 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/18/99 12:52 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4426 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 261 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92294 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/07/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 378.8 GRAMS DATE AND TIME OF NECROPSY: 02/07/00 11:40 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LUNG (LU) : LEFT; VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, VASCULAR,-MINIMAL, >APPEARANCE; THIN; VERIFIED AT FOCAL NECROPSY: NOT OBSERVED KIDNEY (KD) : >SKIN & PELAGE; PALE; ENTIRE BODY; -CAST, PROTEINACEOUS,-MINIMAL, MULTI- VERIFIED AT NECROPSY: NOT APPLICABLE FOCAL >EXCRETION; NO FECES; VERIFIED AT PANCREAS (PA) : NECROPSY: NOT APPLICABLE -HEMORRHAGE,-SLIGHT, MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -B-ADENOMA, ISLET CELL,-NEOPLASM NECROPSY: NOT APPLICABLE PRESENT >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; THYROID (TY) : VERIFIED AT NECROPSY: NOT APPLICABLE -CYST, FOLLICULAR,-SLIGHT, MULTI-FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -DARK FOCUS(I)/AREA(S); BILATERAL, -HYPERTROPHY,-MODERATE, FOCAL CORTICAL SURFACE: SINGLE DARK BROWN AREA ON EACH, EACH IS 2 MM IN DIAMETER PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 11 X 11 MM, RED, DARK -CYST,-PRESENT RED, GRAY, TAN AND SOFT -HEMORRHAGE,-MODERATELY SEVERE, MULTI- FOCAL -B-ADENOMA,-NEOPLASM PRESENT 4427 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 262 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92294 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/07/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 378.8 GRAMS DATE AND TIME OF NECROPSY: 02/07/00 11:40 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); LEFT HIND FOOT: >UNREMARKABLE SINGLE TAN AND DRY CRUSTED AREA, 4 X 2 MM (CAP 19) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4428 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 263 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92295 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 823.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:11 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; OPAQUE; EYES; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT TESTIS (TE) : TESTIS (TE) : -SMALL; LEFT: 19 X 9 X 9 MM (RIGHT IS -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE 18 X 10 X 10 MM); LEFT APPEARS SOFT -MINERALIZATION,-MINIMAL, MULTI-FOCAL >NOTE:>LEFT TESTIS EYE (EY) : EYE (EY) : -WHITE INTRAOCULAR MATERIAL; BILATERAL -CATARACT,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -DIFFUSELY RED; DARK RED -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 4429 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 264 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92295 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 823.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:11 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU) ------------------------------------------------------------------------------------------------------------------------------------ 4430 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 265 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92296 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/12/99 STUDY DAY OF DEATH: 525 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 394.7 GRAMS DATE AND TIME OF NECROPSY: 09/12/99 8:40 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; UNVERIFIED AT LUNG (LU) : NECROPSY -MINERALIZATION, VASCULAR,-MINIMAL, >SKIN & PELAGE; BROWN HAIR COAT; FOCAL VENTRAL-ABDOMINAL; UNVERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY -ROUGH SURFACE, DIFFUSE; BILATERAL -MINERALIZATION, TUBULAR,-MINIMAL, >EXCRETION; NO FECES; UNVERIFIED AT -DIFFUSELY LIGHT; BILATERAL: TAN MULTI-FOCAL NECROPSY -NEPHROPATHY, CHRONIC PROGRESSIVE,- >BEHAVIOR; HYPOACTIVE; UNVERIFIED AT SEVERE, DIFFUSE NECROPSY LIVER (LI) : LIVER (LI) : >EYES; RED DISCHARGE; EYES; UNVERIFIED -LIGHT FOCUS(I)/AREA(S); LEFT LATERAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, AT NECROPSY LOBE: MULTIPLE TAN AREAS, UP TO 15 X 2 MULTI-FOCAL MM; COLLECTED ON ROUTINE SECTION BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 5 X 5 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN, LOBULATED AND SOFT -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; YELLOW, BROWN AND MOIST MATERIAL 4431 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 266 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92296 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/12/99 STUDY DAY OF DEATH: 525 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 394.7 GRAMS DATE AND TIME OF NECROPSY: 09/12/99 8:40 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED DRY MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4432 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 267 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92297 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 640.9 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:43 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; LIMB-HIND- KIDNEY (KD) : LEFT; VERIFIED AT NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, DARK RED, TAN -HEMORRHAGE,-MODERATE, MULTI-FOCAL AND SOFT -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT >NOTE:>ONLY THE CALCULUS WAS PRESENT ON THE SECTION DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); LEFT -FIBROSIS,-MODERATE, FOCAL HINDFOOT: SINGLE DARK RED, TAN AND -INFLAMMATION, CHRONIC ACTIVE,-MINIMAL, MOIST ABRADED AREA, 6 X 5 X 4 MM; MULTI-FOCAL COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN 4433 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 268 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92297 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 640.9 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:43 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4434 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 269 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92298 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 470 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 477.6 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 13:12 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EXCRETION; NO FECES; VERIFIED AT SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -LIGHT FOCUS(I)/AREA(S); LEFT LATERAL -VACUOLATION, HEPATOCELLULAR,- NECROPSY: OBSERVED LOBE: SINGLE TAN AREA, 2 X 1 MM; MODERATELY SEVERE, DIFFUSE >APPEARANCE; HUNCHED; VERIFIED AT COLLECTED ON ROUTINE SECTION NECROPSY: NOT APPLICABLE BRAIN (BR) : BRAIN (BR) : >SKIN & PELAGE; YELLOW HAIR COAT; -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: OBSERVED PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 10 X 7 MM, DARK RED, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL TAN, LOBULATED, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EROSION,-MODERATE, FOCAL MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -THICKENED; ALL REGIONS, DIFFUSE: TAN >TISSUE MISSING AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL LOWER INCISORS 4435 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 270 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92298 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 470 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 477.6 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 13:12 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; PERINEAL: YELLOW AND MOIST -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4436 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 271 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92299 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 574.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 12:56 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL LIVER (LI) : LIVER (LI) : -DARK FOCUS(I)/AREA(S); ALL LOBES: >UNREMARKABLE MULTIPLE BROWN AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 8 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE -EROSION,-SLIGHT, FOCAL DARK BROWN AREA, 3 MM IN DIAMETER URINARY BLADDER (UB) : -CALCULUS,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : -FIBROSIS,-MODERATE, FOCAL 4437 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 272 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92299 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 574.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 12:56 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); RIGHT HINDFOOT: SINGLE DARK RED, TAN ABRADED AREA, 11 X 10 X 5 MM; COLLECTED >NOTE:>LEFT HINDFOOT SORE/SCAB NOT SEEN AS NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4438 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 273 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92300 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 318.2 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:46 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 10.1638 3.1942 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : UNVERIFIED AT NECROPSY -NO MACROSCOPIC LESIONS -------------------------------------------- 4439 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 274 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92300 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 318.2 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:46 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) ------------------------------------------------------------------------------------------------------------------------------------ 4440 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 275 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92301 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/01/99 8:01 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -HYPERPLASIA, TRANSITIONAL CELL,- SLIGHT, DIFFUSE -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, FOCAL -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -CYST, FOLLICULAR,-MINIMAL, FOCAL PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,- URETHRA: GREEN AND FIRM MODERATE, MULTI-FOCAL DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT CAVITY, ABDOM (PC) : >TISSUE MISSING >NOTE:>MASS DESCRIBED GROSSLY WAS A PANCREATIC ISLET CELL ADENOMA 4441 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 276 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92301 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/01/99 8:01 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAVITY, ABDOM (PC) : -MASS(ES); WITHIN ADIPOSE OF ABDOMINAL CAVITY, NEAR MESENTERIC LYMPH NODES, NEAR COLON: SINGLE MASS, 8 X 8 X 7 MM, RED, DARK RED, TAN, FIRM AND NODULAR; CUT SURFACE EXUDES A RED FLUID (CAP 19); REGIONAL LN (MESENTERIC) NORMAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW -DISCHARGE-OCULAR; BILATERAL: RED -DISCHARGE-NASAL; BILATERAL: RED -------------------------------------------- 4442 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 277 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92301 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/01/99 8:01 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), SKIN (SK), LN, MESENTERIC (MS), MAMMARY, MALE (MM) ------------------------------------------------------------------------------------------------------------------------------------ 4443 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 278 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92302 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/02/00 STUDY DAY OF DEATH: 668 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 712.5 GRAMS DATE AND TIME OF NECROPSY: 02/02/00 8:02 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; BROWN HAIR COAT; KIDNEY (KD) : PERINEAL AREA; VERIFIED AT NECROPSY: -INFARCT,-MINIMAL, FOCAL OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EXCRETION; NO FECES; VERIFIED AT MODERATE, MULTI-FOCAL NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >DISCHARGE; NASAL; RED IN COLOR; -LIGHT FOCUS(I)/AREA(S); LEFT MEDIAN -LIPIDOSIS, SUBCAPSULAR (TENSION VERIFIED AT NECROPSY: OBSERVED LOBE: SINGLE TAN AREA, 8 X 5 MM; LIPIDOSIS),-MODERATELY SEVERE, FOCAL >EYES; SQUINTED; PERI-ORBITAL; VERIFIED COLLECTED -VACUOLATION, HEPATOCELLULAR,-MODERATE, AT NECROPSY: NOT APPLICABLE MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >RESPIRATION; LABORED; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE ADRENAL, CORTEX (AC) : >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED -VACUOLATION,-MINIMAL, MULTI-FOCAL AT NECROPSY: NOT APPLICABLE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, RED, DARK -HEMORRHAGE,-MINIMAL, MULTI-FOCAL RED, TAN, SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE >UNREMARKABLE BROWN ERODED AREAS, UP TO 5 X 2 MM PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING >UNREMARKABLE URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT 4444 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 279 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92302 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/02/00 STUDY DAY OF DEATH: 668 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 712.5 GRAMS DATE AND TIME OF NECROPSY: 02/02/00 8:02 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SEMINAL VESICLES (SV) : -LARGE; TRIMMING COMMENT: LEFT: 40 X 15 X 5 MM (RIGHT IS 25 X 11 X 5 MM) DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; RIGHT: 14 X 9 X 5 MM (LEFT IS -DILATATION, SINUSOIDAL,-MODERATELY 5 X 3 X 2 MM); RIGHT APPEARS CYSTIC; SEVERE, MULTI-FOCAL (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; MOIST BROWN MATERIAL -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -DISCHARGE-NASAL; BILATERAL: MOIST CLEAR MATERIAL -------------------------------------------- 4445 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 280 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92302 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/02/00 STUDY DAY OF DEATH: 668 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 712.5 GRAMS DATE AND TIME OF NECROPSY: 02/02/00 8:02 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4446 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 281 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92303 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 685.1 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:01 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0356 .0052 % .0152 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0274 .0040 % .0117 WEIGHT TAKEN BRAIN (BR) 2.3473 .3426 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.9623 .2864 % .8360 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.8960 .2767 % .8077 WEIGHT TAKEN LIVER (LI) 16.8091 2.4535 % 7.1610 WEIGHT TAKEN LUNG (LU) 2.1910 .3198 % .9334 WEIGHT TAKEN SPLEEN (SP) 1.6829 .2456 % .7170 WEIGHT TAKEN LEFT TESTIS (TE0) 1.5796 .2306 % .6729 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.5913 .2323 % .6779 WEIGHT TAKEN LF THYROID/PARA (TT0) .0104 .0015 % .0044 WEIGHT TAKEN RT THYROID/PARA (TT1) .0155 .0023 % .0066 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-SLIGHT, FOCAL SPLEEN (SP) : SPLEEN (SP) : -LIGHT FOCUS(I)/AREA(S); PARIETAL >UNREMARKABLE SURFACE: SINGLE GRAY AREA, 2 MM IN DIAMETER DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 4447 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 282 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92303 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 685.1 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:01 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4448 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 283 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92304 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/31/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 871.9 GRAMS DATE AND TIME OF NECROPSY: 01/31/00 13:00 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); HEAD-MAXILLARY-LEFT; WEEK KIDNEY (KD) : 93; 35 X 30MM; VERIFIED AT NECROPSY: -NEPHROPATHY, CHRONIC PROGRESSIVE,- OBSERVED SLIGHT, MULTI-FOCAL LIVER (LI) : LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN -DEGENERATION, CYSTIC,-MODERATE, MULTI- -DARK FOCUS(I)/AREA(S); ALL LOBES: FOCAL MULTIPLE DARK BROWN FOCI, PINPOINT AND -FOCUS, ALTERED HEPATOCELLULAR, UP TO 1 MM IN DIAMETER EOSINOPHILIC,-MODERATE, MULTI-FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT DEATH COMMENT (DC) : -AUDITORY SEBACEOUS GLAND CARCINOMA,- PRESENT AUDITORY SEB GL (AS) : AUDITORY SEB GL (AS) : -MASS(ES); LEFT: SINGLE MASS, 45 X 35 -M-CARCINOMA,-NEOPLASM PRESENT X 27 MM, RED, DARK RED, TAN, GREEN, LOBULATED, VASCULAR, AND FIRM; ON SECTION EXUDES A LIGHT GREEN, CASEOUS SEMISOLID; COLLECTED INTACT; REGIONAL LN (MANDIBULAR) NORMAL (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL OBESE 4449 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 284 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92304 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/31/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 871.9 GRAMS DATE AND TIME OF NECROPSY: 01/31/00 13:00 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; LEFT: DARK RED, DRY, CRUSTED MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), TESTIS (TE), LN, MANDIBULAR (MN) ------------------------------------------------------------------------------------------------------------------------------------ 4450 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 285 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92305 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 438 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 446.1 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 14:31 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; VENTRAL- KIDNEY (KD) : CERVICAL; VERIFIED AT NECROPSY: -NEPHROPATHY, CHRONIC PROGRESSIVE,- OBSERVED MODERATE, MULTI-FOCAL >SKIN & PELAGE; SORE/SCAB; DORSAL- LIVER (LI) : LIVER (LI) : CERVICAL; VERIFIED AT NECROPSY: -MASS(ES); RIGHT MEDIAN LOBE: SINGLE -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM OBSERVED MASS, 28 X 21 X 12 MM, TAN, RED, PRESENT >SKIN & PELAGE; SORE/SCAB; HEAD-ENTIRE; VASCULAR AND FIRM; SAME ON CUT VERIFIED AT NECROPSY: OBSERVED SURFACE; COLLECTED INTACT; REGIONAL LN >APPEARANCE; HUNCHED; VERIFIED AT (HEPATIC) NOT IDENTIFIED NECROPSY: NOT APPLICABLE -LARGE; ALL LOBES: MARGINS ROUNDED >RESPIRATION; LABORED; VERIFIED AT THYROID (TY) : NECROPSY: NOT APPLICABLE -CYST, ULTIMOBRANCHIAL,-PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT SKIN (SK) : SKIN (SK) : NECROPSY: NOT APPLICABLE -CRUSTED AREA(S); MID VENTRAL -ATROPHY, ADNEXAL,-SLIGHT, FOCAL >BEHAVIOR; ATAXIC; VERIFIED AT CERVICAL, MID DORSAL THORACIC AND -INFLAMMATION, ACUTE,-MODERATE, FOCAL NECROPSY: NOT APPLICABLE HEAD: MULTIPLE TAN CRUSTED AREAS, UP >EXCRETION; FEW FECES; VERIFIED AT TO 5 MM IN DIAMETER (CAP 18); POSSIBLY NECROPSY: NOT APPLICABLE CONSISTENT WITH ALL SORES NOTED IN >APPEARANCE; THIN; VERIFIED AT CLINICAL OBSERVATIONS NECROPSY: OBSERVED BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 5 MM, DARK RED, -HEMORRHAGE,-SEVERE, MULTI-FOCAL TAN AND FIRM -B-ADENOMA,-NEOPLASM PRESENT 4451 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 286 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92305 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 438 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 446.1 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 14:31 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE -EDEMA,-MODERATELY SEVERE, FOCAL -DARK FOCUS(I)/AREA(S); MUCOSA: -INFLAMMATION, ACUTE,-SLIGHT, MULTI- MULTIPLE DARK BROWN AREAS, UP TO 5 X 3 FOCAL MM -ULCERATION,-SEVERE, FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS 6 X 3 X 2 -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MM MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -GI TRACT-DARK FLUID CONTENT; DUODENUM AND JEJUNUM: CONTAINS A BLACK SEMISOLID MATERIAL -------------------------------------------- 4452 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 287 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92305 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 438 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 446.1 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 14:31 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4453 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 288 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92306 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/27/00 7:56 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-SLIGHT, MULTI- FOCAL LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, FOCAL PANCREAS (PA) : PANCREAS (PA) : -MASS(ES); NEAR SPLEEN: SINGLE MASS, 5 -ATROPHY, ACINAR,-MINIMAL, FOCAL X 5 X 4 MM, TAN AND FIRM; SAME ON CUT -B-ADENOMA, ISLET CELL,-NEOPLASM SURFACE (CAP 22); REGIONAL LN PRESENT (PANCREATIC) NOT IDENTIFIED THYROID (TY) : THYROID (TY) : -LARGE; BILATERAL: LEFT IS 6 X 5 X 4 -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM MM, RIGHT IS 5 X 3 X 3 MM PRESENT PARATHYROID (PT) : PARATHYROID (PT) : -LARGE; LEFT: 1 MM IN DIAMETER (RIGHT >UNREMARKABLE IS LESS THAN 1 MM IN DIAMETER) TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 MM IN DIAMETER, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT 4454 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 289 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92306 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/27/00 7:56 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL TO URETHRA: TAN, -INFLAMMATION, CHRONIC-ACTIVE,- GREEN AND FIRM MODERATE, MULTI-FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU) ------------------------------------------------------------------------------------------------------------------------------------ 4455 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 290 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92307 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/12/00 STUDY DAY OF DEATH: 707 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 447.0 GRAMS DATE AND TIME OF NECROPSY: 03/12/00 9:33 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); DORSAL; WEEK 59; LUNG (LU) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, GRANULOMATOUS,-SLIGHT, >APPEARANCE; THIN; VERIFIED AT MULTI-FOCAL NECROPSY: OBSERVED -MINERALIZATION,-MINIMAL, MULTI-FOCAL >WART LIKE LESION(S); NOSE; WEEK 100; KIDNEY (KD) : VERIFIED AT NECROPSY: NOT OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >MASS(ES); HEAD-MAXILLARY-LEFT; WEEK SLIGHT, MULTI-FOCAL 100; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : >RESPIRATION; IRREGULAR; VERIFIED AT -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >RESPIRATION; AUDIBLE; VERIFIED AT PANCREAS (PA) : PANCREAS (PA) : NECROPSY: NOT APPLICABLE -MASS(ES); NEAR SPLEEN: SINGLE MASS, 3 -B-ADENOMA, ISLET CELL,-NEOPLASM MM IN DIAMETER, TAN AND SOFT (CAP 28); PRESENT REGIONAL LN (MESENTERIC) NORMAL THYROID (TY) : THYROID (TY) : -MASS(ES); INVOLVING ENTIRE LEFT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT THYROID: SINGLE MASS, 7 X 5 X 4 MM, TAN AND FIRM; COLLECTED INTACT; REGIONAL LN (MEDIASTINAL) NORMAL (CAP 26) SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); DORSAL LUMBAR -B-KERATOACANTHOMA,- 2 NEOPLASMS REGION: TWO DRY TAN CRUSTED AREAS, ONE PRESENT IS 15 X 12 MM (CAP 22), THE OTHER IS 4 MM IN DIAMETER (CAP 23) 4456 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 291 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92307 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/12/00 STUDY DAY OF DEATH: 707 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 447.0 GRAMS DATE AND TIME OF NECROPSY: 03/12/00 9:33 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 10 X 6 MM, DARK BROWN, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- DARK RED AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK BROWN AREAS, PINPOINT AND UP TO 1 MM IN DIAMETER PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -HEMORRHAGE,-MINIMAL, FOCAL URETHRA: GREENISH-TAN -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: TAN -EDEMA,-SLIGHT, MULTI-FOCAL DEATH COMMENT (DC) : -OSTEOGENIC SARCOMA,-PRESENT CALVARIUM/SKULL (CA) : CALVARIUM/SKULL (CA) : -MASS(ES); LEFT NASAL REGION: SINGLE -M-SARCOMA, OSTEOGENIC,-NEOPLASM MASS, 10 X 10 X 5 MM, TAN AND FIRM; PRESENT SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (MANDIBULAR) NORMAL (CAP 24) ADIPOSE TISSUE (AT) : -FAT NECROSIS,-MODERATE, MULTI-FOCAL 4457 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 292 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92307 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/12/00 STUDY DAY OF DEATH: 707 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 447.0 GRAMS DATE AND TIME OF NECROPSY: 03/12/00 9:33 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADIPOSE TISSUE (AT) : -MASS(ES); NEAR LEFT CAUDAL EPIDIDYMIS: SINGLE MASS, 5 X 5 X 3 MM, TAN AND SOFT; SAME ON CUT SURFACE (CAP 25); REGIONAL LN (ILIAC) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -GI TRACT-LARGE LUMEN; STOMACH: UP TO 50 X 30 X 30 MM, DISTENDED WITH GAS -DISCHARGE-OCULAR; LEFT: MOIST DARK BROWN MATERIAL -DISCHARGE-NASAL; MOIST BROWN MATERIAL -ANIMAL THIN -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR: BROKEN AT BASE -------------------------------------------- 4458 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 293 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92307 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/12/00 STUDY DAY OF DEATH: 707 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 447.0 GRAMS DATE AND TIME OF NECROPSY: 03/12/00 9:33 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LN, MESENTERIC (MS), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML) ------------------------------------------------------------------------------------------------------------------------------------ 4459 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 294 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92308 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 535.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:41 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT LUNG (LU) : LUNG (LU) : NECROPSY: NOT OBSERVED -MOTTLED; ALL LOBES: DARK RED AND TAN -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, MULTI-FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT KIDNEY (KD) : KIDNEY (KD) : -GRANULAR MATERIAL; BILATERAL: -CAST, PROTEINACEOUS,-MINIMAL, MULTI- MULTIPLE TAN AND FIRM GRANULAR FOCAL MATERIAL, UP TO 1 MM IN DIAMETER -DILATATION, PELVIC,-PRESENT -DIFFUSELY LIGHT; BILATERAL: TAN -MINERALIZATION, PELVIC,-MODERATELY SEVERE, MULTI-FOCAL LIVER (LI) : LIVER (LI) : -MOTTLED; ALL LOBES: DARK RED AND TAN -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO -LARGE; ALL LOBES: MARGINS ROUNDED NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PANCREAS (PA) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 4460 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 295 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92308 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 535.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:41 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), TESTIS (TE), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 4461 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 296 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92309 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 558.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:50 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: NOT OBSERVED -DIFFUSELY LIGHT; BILATERAL: TAN -NEPHROPATHY, CHRONIC PROGRESSIVE,- SEVERE, DIFFUSE LIVER (LI) : LIVER (LI) : -MASS(ES); DISTAL END OF LEFT LATERAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL LOBE: SINGLE MASS, 45 X 45 X 25 MM, -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- TAN, RED, VASCULAR AND FIRM; SAME ON FOCAL CUT SURFACE; ENTIRE LOBE COLLECTED; -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- REGIONAL LN (HEPATIC) NORMAL (CAP 22) FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL -FIBROSIS,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT TESTIS (TE) : -MINERALIZATION,-MINIMAL, FOCAL STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -EDEMA,-MODERATELY SEVERE, FOCAL -EROSION,-MINIMAL, FOCAL URINARY BLADDER (UB) : -CALCULUS,-PRESENT 4462 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 297 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92309 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 558.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:50 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, OTHER (LN) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL >NOTE:>HEPATIC LYMPH NODE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA) ------------------------------------------------------------------------------------------------------------------------------------ 4463 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 298 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92310 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/25/99 STUDY DAY OF DEATH: 507 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/25/99 12:48 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -LARGE PELVIS(ES); BILATERAL -CAST, PROTEINACEOUS,-SLIGHT, MULTI- FOCAL -DILATATION, PELVIC,-PRESENT LIVER (LI) : -NECROSIS, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL THYMUS (TH) : THYMUS (TH) : -LARGE; 15 X 9 X 5 MM -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PROSTATE (PR) : PROSTATE (PR) : -MASS(ES); INVOLVING ENTIRE PROSTATE: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO SINGLE MASS, 32 X 23 X 16 MM, TAN, NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT RED, DARK RED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE LUMBAR) DEATH COMMENT (DC) : -HISTIOCYTIC SARCOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -MASS(ES); INVOLVING BILATERAL LUMBAR -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO AND ILIAC LN: SINGLE MASS, 35 X 28 X 9 NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MM, TAN, RED AND GREEN; SAME ON CUT SURFACE; COLLECTED 4464 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 299 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92310 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/25/99 STUDY DAY OF DEATH: 507 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/25/99 12:48 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4465 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 300 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92311 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1008.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:20 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-SLIGHT, MULTI- FOCAL LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); LEFT LATERAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- LOBE: MULTIPLE DARK RED AREAS, UP TO 5 FOCAL MM IN DIAMETER; SOME ARE SUNKEN WITH A -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- STELLATE PATTERN; COLLECTED ON ROUTINE FOCAL SECTION TESTIS (TE) : TESTIS (TE) : -RED/YELLOW FOCUS(I)/AREA(S); LEFT, -ATROPHY/DEGENERATION,-SLIGHT, MULTI- BENEATH TUNICA: MULTIPLE AREAS, UP TO FOCAL 8 X 5 MM -CYST,-PRESENT -INFLAMMATION, CHRONIC,-MODERATE, MULTI- FOCAL -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); MID ASPECT: -HEMORRHAGE,-MODERATE, FOCAL SINGLE DARK RED AREA, 1 MM IN DIAMETER -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE >UNREMARKABLE URINARY BLADDER (UB) : -CALCULUS,-PRESENT 4466 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 301 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92311 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1008.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:20 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 4467 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 302 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92312 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 449 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 455.9 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 13:25 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT DEATH COMMENT (DC) : NECROPSY: NOT OBSERVED -UNDETERMINED,-PRESENT >APPEARANCE; HUNCHED; VERIFIED AT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : NECROPSY: NOT APPLICABLE -CRUSTED AREA(S); BILATERAL, HINDFEET: -EROSION,-MODERATE, FOCAL >SKIN & PELAGE; SORE/SCAB; PAW-HIND- SINGLE RED, TAN AND DRY AREA ON EACH, -FIBROSIS,-MODERATE, FOCAL LEFT; VERIFIED AT NECROPSY: OBSERVED BOTH ARE 4 MM IN DIAMETER; BOTH -INFLAMMATION, ACUTE,-MODERATE, FOCAL >APPEARANCE; MALOCCLUSION; VERIFIED AT COLLECTED NECROPSY: OBSERVED GENERAL COMMENT (GC) : >SKIN & PELAGE; ROUGH HAIR COAT; -BLOOD FILM TAKEN VERIFIED AT NECROPSY: NOT APPLICABLE -MALOCCLUSION; BILATERAL, UPPER >EXCRETION; FEW FECES; VERIFIED AT INCISORS NECROPSY: NOT APPLICABLE -------------------------------------------- 4468 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 303 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92312 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 449 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 455.9 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 13:25 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4469 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 304 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92313 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/08/00 STUDY DAY OF DEATH: 674 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/08/00 7:36 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 72; 35 X 40MM; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI- FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, FOCAL SKIN (SK) : SKIN (SK) : -MASS(ES); DORSAL LUMBAR REGION: -B-KERATOACANTHOMA,-NEOPLASM PRESENT SINGLE MASS, 50 X 38 X 15 MM, RED, DARK RED, TAN, FRIABLE AND FIRM; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (INGUINAL) NOT IDENTIFIED DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); VENTRAL CERVICAL REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL MULTIPLE TAN, WHITE AND CREAMY SEMI- FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER (CAP 19) -------------------------------------------- 4470 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 305 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92313 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/08/00 STUDY DAY OF DEATH: 674 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/08/00 7:36 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4471 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 306 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92314 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 515.5 GRAMS DATE AND TIME OF NECROPSY: 01/14/00 8:23 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; DORSAL; LUNG (LU) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, VASCULAR,-MINIMAL, >EXCRETION; FEW FECES; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >APPEARANCE; THIN; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: OBSERVED MODERATE, DIFFUSE >APPEARANCE; MALOCCLUSION; VERIFIED AT THYROID (TY) : NECROPSY: OBSERVED -CYST, FOLLICULAR,-MODERATE, FOCAL >SKIN & PELAGE; RED HAIR COAT; HEAD- -HYPERPLASIA, FOLLICULAR CELL,- ENTIRE; VERIFIED AT NECROPSY: NOT MODERATE, FOCAL OBSERVED SKIN (SK) : SKIN (SK) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -CRUSTED AREA(S); MID DORSAL THORACIC -CYST, EPIDERMAL INCLUSION,-PRESENT NECROPSY: NOT APPLICABLE REGION: SINGLE DARK RED AND DRY AREA, 7 X 6 X 4 MM (CAP 19) BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 9 X 9 MM, DARK RED, -CYST,-PRESENT RED, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 4472 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 307 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92314 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 515.5 GRAMS DATE AND TIME OF NECROPSY: 01/14/00 8:23 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, UPPER INCISORS -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4473 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 308 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92315 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 731.3 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:24 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0306 .0042 % .0132 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0311 .0043 % .0135 WEIGHT TAKEN BRAIN (BR) 2.3117 .3161 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.8457 .2524 % .7984 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.9516 .2669 % .8442 WEIGHT TAKEN LIVER (LI) 19.3215 2.6421 % 8.3581 WEIGHT TAKEN LUNG (LU) 2.2745 .3110 % .9839 WEIGHT TAKEN SPLEEN (SP) .9082 .1242 % .3929 WEIGHT TAKEN LEFT TESTIS (TE0) 1.8018 .2464 % .7794 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.7817 .2436 % .7707 WEIGHT TAKEN LF THYROID/PARA (TT0) .0166 .0023 % .0072 WEIGHT TAKEN RT THYROID/PARA (TT1) .0199 .0027 % .0086 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 4474 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 309 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92315 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 731.3 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:24 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4475 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 310 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92316 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/07/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 512.6 GRAMS DATE AND TIME OF NECROPSY: 03/07/00 12:56 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; VENTRAL- KIDNEY (KD) : KIDNEY (KD) : ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: -DIFFUSELY LIGHT; BILATERAL: TAN >UNREMARKABLE OBSERVED LIVER (LI) : LIVER (LI) : >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; -DIFFUSELY LIGHT; ALL LOBES: TAN >UNREMARKABLE WEEK 84; 30 X 40MM; VERIFIED AT SKIN (SK) : NECROPSY: OBSERVED -EROSION,-MODERATELY SEVERE, FOCAL >EYES; PALE; EYES; VERIFIED AT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE -INFLAMMATION, ACUTE,-MODERATE, FOCAL >APPEARANCE; HUNCHED; VERIFIED AT -M-FIBROSARCOMA,-NEOPLASM PRESENT NECROPSY: NOT APPLICABLE HEART (HT) : HEART (HT) : >SKIN & PELAGE; PALE; ENTIRE BODY; -LIGHT FOCUS(I)/AREA(S); PERICARDIUM, -DEGENERATION/NECROSIS,-SLIGHT, FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE DIFFUSE: MULTIPLE GREY AREAS, UP TO 10 -FIBROSIS,-SLIGHT, FOCAL X 6 MM SPLEEN (SP) : SPLEEN (SP) : -LARGE; 63 X 16 X 10 MM -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE, DIFFUSE STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE -EROSION,-MINIMAL, FOCAL DARK BROWN AREA, 1 MM IN DIAMETER DEATH COMMENT (DC) : -FIBROSARCOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -DILATATION, SINUSOIDAL,-MODERATE, FILLED CYST, 8 X 5 X 5 MM (CAP 23) MULTI-FOCAL 4476 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 311 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92316 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/07/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 512.6 GRAMS DATE AND TIME OF NECROPSY: 03/07/00 12:56 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); RIGHT VENTRAL ABDOMINAL >TISSUE MISSING REGION: SINGLE MASS, 40 X 40 X 20 MM, >NOTE:>MAMMARY MASS DESCRIBED GROSSLY TAN, RED, FIRM, AND LOBULATED; SAME ON WAS A SUBCUTANEOUS FIBROSARCOMA CUT SURFACE; COLLECTED INTACT; ALL OVERLYING SKIN IS RED, MOIST, AND ULCERATED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4477 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 312 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92317 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 834.6 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:24 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0336 .0040 % .0130 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0277 .0033 % .0107 WEIGHT TAKEN BRAIN (BR) 2.5770 .3088 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.7254 .3266 % 1.0576 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.7560 .3302 % 1.0695 WEIGHT TAKEN LIVER (LI) 26.2758 3.1483 % 10.1963 WEIGHT TAKEN LUNG (LU) 2.2535 .2700 % .8745 WEIGHT TAKEN SPLEEN (SP) 1.4602 .1750 % .5666 WEIGHT TAKEN LEFT TESTIS (TE0) 1.9470 .2333 % .7555 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.9777 .2370 % .7674 WEIGHT TAKEN LF THYROID/PARA (TT0) .0163 .0020 % .0063 WEIGHT TAKEN RT THYROID/PARA (TT1) .0323 .0039 % .0125 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, MULTI- FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 4478 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 313 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92317 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 834.6 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:24 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4479 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 314 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92318 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 789.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:48 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NONFORMED FECES; VERIFIED KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -DEGENERATION, CYSTIC,-SLIGHT, FOCAL MULTIPLE DARK RED AREAS, UP TO 3 MM IN -VACUOLATION, HEPATOCELLULAR,-SLIGHT, DIAMETER; COLLECTED ON ROUTINE MULTI-FOCAL SECTIONS -DIFFUSELY LIGHT; ALL LOBES: TAN PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT TESTIS (TE) : TESTIS (TE) : -SMALL; RIGHT: 9 X 10 X 18 MM (LEFT IS -ATROPHY/DEGENERATION,-SEVERE, MULTI- 13 X 14 X 25 MM) FOCAL -RED/YELLOW FOCUS(I)/AREA(S); LEFT, -MINERALIZATION,-SLIGHT, MULTI-FOCAL BENEATH TUNICA: MULTIPLE AREAS, UP TO -B-INTERSTITIAL CELL TUMOR,-NEOPLASM 5 X 7 MM PRESENT PITUITARY (PI) : PITUITARY (PI) : -CYST(S); LEFT ASPECT: SINGLE CYST, 2 -HEMORRHAGE,-SLIGHT, MULTI-FOCAL MM IN DIAMETER, FILLED WITH A DARK RED -B-ADENOMA,-NEOPLASM PRESENT FLUID URINARY BLADDER (UB) : -CALCULUS,-PRESENT 4480 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 315 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92318 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 789.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:48 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU) ------------------------------------------------------------------------------------------------------------------------------------ 4481 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 316 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92319 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 649.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:09 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >MASS(ES); VENTRAL-THORACIC-RIGHT; WEEK FOCAL 82; 10 X 10MM; VERIFIED AT NECROPSY: PANCREAS (PA) : OBSERVED -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT TESTIS (TE) : -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT SKIN (SK) : SKIN (SK) : -MASS(ES); RIGHT THORACIC REGION: -ATROPHY, ADNEXAL,-MODERATE, FOCAL SINGLE MASS, 15 MM IN DIAMETER, TAN -B-FIBROMA,-NEOPLASM PRESENT AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 23) PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: -HEMORRHAGE,-MODERATE, MULTI-FOCAL SINGLE DARK RED AREA, 2 MM IN DIAMETER -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4482 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 317 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92319 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 649.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:09 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), URINARY BLADDER (UB), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 4483 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 318 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92320 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 717.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:15 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0399 .0056 % .0167 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0353 .0049 % .0148 WEIGHT TAKEN BRAIN (BR) 2.3881 .3331 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.9368 .2701 % .8110 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.1021 .2932 % .8802 WEIGHT TAKEN LIVER (LI) 18.0864 2.5225 % 7.5736 WEIGHT TAKEN LUNG (LU) 1.8416 .2568 % .7712 WEIGHT TAKEN SPLEEN (SP) 1.0452 .1458 % .4377 WEIGHT TAKEN LEFT TESTIS (TE0) 1.6298 .2273 % .6825 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.5695 .2189 % .6572 WEIGHT TAKEN LF THYROID/PARA (TT0) .0240 .0033 % .0100 WEIGHT TAKEN RT THYROID/PARA (TT1) .0300 .0042 % .0126 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-SLIGHT, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, FOCAL 4484 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 319 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92320 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 717.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:15 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM; APPEARS SWOLLEN -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4485 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 320 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92321 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 570.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:00 PROSECTOR: JAMES T. CALLEN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; VENTRAL- KIDNEY (KD) : ABDOMINAL; VERIFIED AT NECROPSY: -NEPHROPATHY, CHRONIC PROGRESSIVE,- OBSERVED SLIGHT, MULTI-FOCAL LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -DEGENERATION, CYSTIC,-MODERATE, FOCAL MULTIPLE DARK RED AREAS, UP TO 5 X 3 -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- MM; COLLECTED ON ROUTINE SECTIONS FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL -CYST, ULTIMOBRANCHIAL,-PRESENT SKIN (SK) : SKIN (SK) : -ABRASION(S)/ULCERATION(S); MID -EROSION,-MODERATE, FOCAL PERIANAL REGION: MULTIPLE TAN, MOIST -FIBROSIS,-MODERATE, FOCAL ABRADED AREAS, UP TO 4 MM IN DIAMETER -INFLAMMATION, CHRONIC-ACTIVE,- (CAP 22) MODERATELY SEVERE, MULTI-FOCAL URINARY BLADDER (UB) : -CALCULUS,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 4486 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 321 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92321 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 570.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:00 PROSECTOR: JAMES T. CALLEN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4487 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 322 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92322 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 764.6 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:17 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-SLIGHT, MULTI- FOCAL LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL THYROID (TY) : -HYPERPLASIA, FOLLICULAR CELL,- MODERATE, FOCAL TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN 4488 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 323 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92322 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 764.6 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:17 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 4489 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 324 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92323 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 819.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:04 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; LIMITED USE; LIMB-HIND- KIDNEY (KD) : KIDNEY (KD) : LEFT; VERIFIED AT NECROPSY: NOT -LARGE PELVIS(ES); LEFT -DILATATION, PELVIC,-PRESENT APPLICABLE -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- -LARGE; BILATERAL: EACH ARE 30 X 20 X MODERATELY SEVERE, DIFFUSE 14 MM LIVER (LI) : LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); LEFT LATERAL -FIBROSIS, CAPSULAR,-SLIGHT, FOCAL LOBE: SINGLE TAN LINEAR AREA, 8 X 2 -FOCUS, ALTERED HEPATOCELLULAR, MM; COLLECTED ON ROUTINE SECTION EOSINOPHILIC,-MODERATE, MULTI-FOCAL -RED FOCUS(I)/AREA(S); ALL LOBES: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO MULTIPLE DARK RED AREAS, UP TO 3 MM IN NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT DIAMETER; COLLECTED ON ROUTINE SECTIONS PANCREAS (PA) : PANCREAS (PA) : -MASS(ES); DIFFUSE: MULTIPLE MASSES, -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL UP TO 10 MM IN DIAMETER, TAN AND FIRM; -PIGMENT,-MINIMAL, MULTI-FOCAL SAME ON CUT SURFACE (CAP 24); REGIONAL -B-ADENOMA, ACINAR CELL,-NEOPLASM LN (SEE PANCREATIC) PRESENT -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : THYROID (TY) : -LARGE; BILATERAL: EACH IS 9 X 5 X 3 >UNREMARKABLE MM 4490 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 325 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92323 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 819.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:04 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TESTIS (TE) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL SPLEEN (SP) : SPLEEN (SP) : -LARGE; 51 X 14 X 8 MM >UNREMARKABLE STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE -EROSION,-MINIMAL, FOCAL DARK BROWN LINEAR AREA, 3 X 1 MM -THICKENED WALL; MUCOSA, DIFFUSE DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT AORTA (AO) : AORTA (AO) : -LARGE; DIFFUSE: 3 MM IN DIAMETER; >UNREMARKABLE COLLECTED LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; LEFT, LUMBAR: 15 X 9 X 4 MM -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO (RIGHT NOT IDENTIFIED) (CAP 26) NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT BILE DUCT (BD) : BILE DUCT (BD) : -LARGE; DIFFUSE: 2 MM IN DIAMETER (CAP >UNREMARKABLE 23) LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -DIFFUSELY RED; DARK RED (CAP 25) -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT, MULTI-FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 4491 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 326 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92323 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 819.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:04 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), LN, RENAL (RL), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), AUDITORY SEB GL (AS), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 4492 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 327 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92324 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 671.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 9:46 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0297 .0044 % .0125 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0263 .0039 % .0111 WEIGHT TAKEN BRAIN (BR) 2.3771 .3543 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 2.4922 .3714 % 1.0484 WEIGHT TAKEN RIGHT KIDNEY (KD1) 2.7645 .4120 % 1.1630 WEIGHT TAKEN LIVER (LI) 20.7937 3.0989 % 8.7475 WEIGHT TAKEN LUNG (LU) 2.1598 .3219 % .9086 WEIGHT TAKEN SPLEEN (SP) 1.2150 .1811 % .5111 WEIGHT TAKEN LEFT TESTIS (TE0) 1.8989 .2830 % .7988 WEIGHT TAKEN RIGHT TESTIS (TE1) 1.9085 .2844 % .8029 WEIGHT TAKEN LF THYROID/PARA (TT0) .0190 .0028 % .0080 WEIGHT TAKEN RT THYROID/PARA (TT1) .0172 .0026 % .0072 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4493 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 328 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92324 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 671.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 9:46 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 4494 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 329 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92325 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 591.5 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:48 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 20.3850 3.4463 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : VERIFIED AT NECROPSY: OBSERVED -NO MACROSCOPIC LESIONS -------------------------------------------- 4495 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 330 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92325 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 591.5 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:48 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) ------------------------------------------------------------------------------------------------------------------------------------ 4496 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 331 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92326 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 491.6 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:37 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : VERIFIED AT NECROPSY: OBSERVED -NO MACROSCOPIC LESIONS -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) ------------------------------------------------------------------------------------------------------------------------------------ 4497 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 332 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92327 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 489.6 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:13 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 14.0186 2.8633 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : VERIFIED AT NECROPSY: OBSERVED -NO MACROSCOPIC LESIONS -------------------------------------------- 4498 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 333 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92327 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 489.6 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:13 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) ------------------------------------------------------------------------------------------------------------------------------------ 4499 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 334 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92328 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 492.6 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:54 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 15.1771 3.0810 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : VERIFIED AT NECROPSY: OBSERVED -NO MACROSCOPIC LESIONS -------------------------------------------- 4500 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 335 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92328 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 492.6 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:54 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) ------------------------------------------------------------------------------------------------------------------------------------ 4501 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 336 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92329 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 285.4 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:16 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 10.4029 3.6450 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : UNVERIFIED AT NECROPSY -NO MACROSCOPIC LESIONS -------------------------------------------- 4502 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 337 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92329 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 285.4 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:16 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) ------------------------------------------------------------------------------------------------------------------------------------ 4503 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 338 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92330 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 560.7 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:54 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 17.2000 3.0676 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LEFT TESTIS (TE0) -------- -------- -------- NOT TAKEN RIGHT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : VERIFIED AT NECROPSY: OBSERVED -NO MACROSCOPIC LESIONS -------------------------------------------- 4504 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 339 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92330 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 560.7 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:54 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) ------------------------------------------------------------------------------------------------------------------------------------ 4505 Covance 6329-212 Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Author: Peter J. Thomford, PhD Study Completion Date: December 13, 2001 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-228 Sponsor Study Identification: 3M T-6316.1 Volume XII of XII Page 4506 of 4873 Covance 6329-212 Covance 6329-228 3M T-6316.1 VOLUME XII OF XII CONTENTS Page APPENDIX 6 (Continued) ........................................................................................ 4508 Individual Anatomic Pathology Data ..................................................................... 4509 APPENDIX 7............................................................................................................ 4857 Cell Proliferation Report ....................................................................................... 4858 4507 APPENDIX 6 (Continued) Individual Anatomic Pathology Data Covance 6329-212 Covance 6329-228 3M T-6316.1 4508 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 340 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92331 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/06/00 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 273.0 GRAMS DATE AND TIME OF NECROPSY: 01/06/00 15:06 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK KIDNEY (KD) : 42; 60 X 40MM; VERIFIED AT NECROPSY: -CAST, PROTEINACEOUS,-MINIMAL, MULTI- OBSERVED FOCAL >APPEARANCE; THIN; VERIFIED AT -MINERALIZATION, PELVIC,-MINIMAL, MULTI- NECROPSY: OBSERVED FOCAL >APPEARANCE; HUNCHED; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -MOTTLED; ALL LLOBES: TAN AND FIRM -VACUOLATION, HEPATOCELLULAR,-MODERATE, >EXCRETION; NO FECES; VERIFIED AT MULTI-FOCAL NECROPSY: NOT APPLICABLE PARATHYROID (PT) : >SKIN & PELAGE; YELLOW HAIR COAT; >SECTION EXAMINED; TISSUE NOT PRESENT VERIFIED AT NECROPSY: OBSERVED MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : >RESPIRATION; IRREGULAR; VERIFIED AT -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE SINGLE MASS, 75 X 40 X 15 MM, RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; DARK RED, GREEN, TAN, FIRM AND -PIGMENT,-MODERATELY SEVERE, MULTI- VERIFIED AT NECROPSY: NOT APPLICABLE LOBULATED; SAME ON CUT SURFACE; FOCAL >EYES; SQUINTED; PERI-ORBITAL; VERIFIED COLLECTED; REGIONAL LN (AXILLARY) NOT -B-ADENOMA,- 2 NEOPLASMS PRESENT AT NECROPSY: NOT APPLICABLE IDENTIFIED >BEHAVIOR; HYPOACTIVE; VERIFIED AT -MASS #2; LEFT INGUINAL REGION: SINGLE NECROPSY: NOT APPLICABLE MASS, 35 X 25 X 25 MM, RED, DARK RED, TAN AND BROWN; ON SECTION, EXUDES A BROWN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED EYE (EY) : -INFLAMMATION, ACUTE, CILIARY,- MODERATELY SEVERE, FOCAL 4509 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 341 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92331 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/06/00 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 273.0 GRAMS DATE AND TIME OF NECROPSY: 01/06/00 15:06 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT MUSCLE, SKELETAL (SM) : -MINERALIZATION,-SLIGHT, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY RED; DARK RED -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -THROMBUS,-MINIMAL, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MODERATE, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 8 X 6 MM, RED, DARK -HEMORRHAGE,-MODERATELY SEVERE, MULTI- RED, GREY, TAN AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE GREEN MATERIAL 4510 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 342 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92331 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/06/00 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 273.0 GRAMS DATE AND TIME OF NECROPSY: 01/06/00 15:06 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL; COLLECTED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; DARK YELLOW AND BROWN -DISCHARGE-OCULAR; BILATERAL: RED -ANIMAL THIN -------------------------------------------- 4511 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 343 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92331 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/06/00 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 273.0 GRAMS DATE AND TIME OF NECROPSY: 01/06/00 15:06 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), NERVE, SCIATIC (SN), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4512 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 344 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92332 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 185.7 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:56 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.0862 3.8159 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : UNVERIFIED AT NECROPSY -NO MACROSCOPIC LESIONS -------------------------------------------- 4513 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 345 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92332 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 185.7 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:56 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4514 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 346 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92333 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 580.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:20 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LUNG (LU) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 9 X 6 X 6 MM (LEFT IS 4 -DEGENERATION, CYSTIC, CORTICAL,- X 4 X 2 MM) SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL RECTUM (RE) : -PARASITISM,-PRESENT UTERUS (UT) : -DILATATION,-MINIMAL, FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE DARK RED FLUID -CYST,-PRESENT FILLED CYST, 4 MM IN DIAMETER DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4515 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 347 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92333 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 580.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:20 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4516 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 348 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92334 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 656.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:21 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LUNG (LU) : VERIFIED AT NECROPSY: OBSERVED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC >UNREMARKABLE SURFACE OF RIGHT MEDIAN LOBE: SINGLE DARK RED AREA, 4 X 3 MM; COLLECTED ON ROUTINE SECTION THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 7 X 6 X 3 MM -B-ADENOMA,-NEOPLASM PRESENT -DIFFUSELY RED; DARK RED STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE BLACK AREAS, UP TO 3 X 2 MM 4517 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 349 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92334 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 656.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:21 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4518 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 350 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92335 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/29/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/29/98 13:05 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; MOUTH; VERIFIED KIDNEY (KD) : AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >SACRIFICE; SACRIFICE APPROVED BY; FOCAL VETERINARIAN; VERIFIED AT NECROPSY: DEATH COMMENT (DC) : NOT APPLICABLE -ORAL EROSION,-PRESENT CAVITY, ORAL (OC) : CAVITY, ORAL (OC) : -ABRASION(S)/ULCERATION(S); TISSUE -EDEMA,-MODERATE, FOCAL SURROUNDING BILATERAL, UPPER INCISORS: -EROSION,-MODERATE, FOCAL SINGLE DARK RED ABRADED AREA, 5 X 4 -HEMORRHAGE,-MODERATE, MULTI-FOCAL MM; TISSUE APPEARS SWOLLEN; COLLECTED -INFLAMMATION, ACUTE,-SLIGHT, MULTI- FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: UPPER INCISORS -------------------------------------------- 4519 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 351 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92335 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/29/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/29/98 13:05 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4520 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 352 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92336 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 570 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 358.9 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 14:40 PROSECTOR: JOHN S. HALFORD RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK KIDNEY (KD) : 59; 60 X 70MM; VERIFIED AT NECROPSY: -CAST, PROTEINACEOUS,-MINIMAL, MULTI- OBSERVED FOCAL >MASS(ES); AXILLARY REGION-RIGHT; WEEK -MINERALIZATION, PELVIC,-SLIGHT, MULTI- 75; 30 X 35MM; VERIFIED AT NECROPSY: FOCAL OBSERVED LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; SORE/SCAB; AXILLARY -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- REGION-LEFT; VERIFIED AT NECROPSY: SURFACE OF LEFT MEDIAN LOBE, ALONG FOCAL OBSERVED MARGIN: SINGLE TAN AREA, 6 X 4 MM (CAP -NECROSIS, HEPATOCELLULAR,-MINIMAL, >SKIN & PELAGE; SORE/SCAB; AXILLARY 20) MULTI-FOCAL REGION-RIGHT; VERIFIED AT NECROPSY: -PIGMENT,-MINIMAL, MULTI-FOCAL OBSERVED THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL SINGLE MASS, 46 X 35 X 21 MM, RED, -INFLAMMATION, ACUTE,-MODERATELY DARK RED, GRAY, TAN, VASCULAR, SEVERE, MULTI-FOCAL LOBULATED, AND FIRM; SAME ON CUT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 19); OVERLYING SKIN HAS A SINGLE, DRY, TAN, AND BROWN ULCERATED AREA, 32 X 32 MM; COLLECTED INTACT WITH MASS 4521 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 353 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92336 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 570 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 358.9 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 14:40 PROSECTOR: JOHN S. HALFORD RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 8 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM (CAP 18) -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 35 X 21 X 18 MM, GRAY, TAN, LOBULATED, AND SOFT; ON SECTION, EXUDES A GREEN CASEOUS MATERIAL; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 19); OVERLYING SKIN HAS A SINGLE DRY, BROWN CRUSTED AREA, 20 X 18 MM; COLLECTED INTACT WITH MASS BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 MM IN DIAMETER (RIGHT -DEGENERATION, CYSTIC, CORTICAL,- IS 4 X 4 X 3 MM) SEVERE, FOCAL -HEMATOPOIESIS, EXTRAMEDULLARY,- MINIMAL, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL 4522 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 354 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92336 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 570 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 358.9 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 14:40 PROSECTOR: JOHN S. HALFORD RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, LOBULATED, AND SOFT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 4523 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 355 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92336 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 570 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 358.9 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 14:40 PROSECTOR: JOHN S. HALFORD RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4524 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 356 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92337 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/26/99 STUDY DAY OF DEATH: 569 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 410.3 GRAMS DATE AND TIME OF NECROPSY: 10/26/99 14:35 PROSECTOR: JOHN S. HALFORD RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL; WEEK 50; KIDNEY (KD) : 35 X 30MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MODERATE, OBSERVED MULTI-FOCAL >MASS(ES); INGUINAL REGION-RIGHT; WEEK LIVER (LI) : LIVER (LI) : 76; VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: >UNREMARKABLE >EYES; RED DISCHARGE; EYE-LEFT; MULTIPLE DARK RED AREAS, UP TO 4 X 3 VERIFIED AT NECROPSY: OBSERVED MM; COLLECTED ON ROUTINE SECTIONS >APPEARANCE; HEAD TILT; LEFT; VERIFIED THYROID (TY) : AT NECROPSY: NOT APPLICABLE -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL SINGLE MASS, 45 X 30 X 21 MM, RED, -MINERALIZATION,-MINIMAL, MULTI-FOCAL DARK RED, GRAY, TAN, LOBULATED AND -NECROSIS,-MODERATELY SEVERE, FOCAL FIRM; SAME ON CUT SURFACE; COLLECTED -PIGMENT,-MODERATE, MULTI-FOCAL INTACT; REGIONAL LN (SEE INGUINAL) -M-CARCINOMA,-NEOPLASM PRESENT -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM (CAP 18); POSSIBLY CONSISTENT WITH MASS (RIGHT INGUINAL REGION) AS NOTED IN CLINICAL OBSERVATIONS BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT 4525 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 357 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92337 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/26/99 STUDY DAY OF DEATH: 569 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 410.3 GRAMS DATE AND TIME OF NECROPSY: 10/26/99 14:35 PROSECTOR: JOHN S. HALFORD RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -SMALL; BILATERAL: EACH IS 4 X 3 X 3 -ANGIECTASIS,-MINIMAL, MULTI-FOCAL MM; RIGHT IS GELATINOUS -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, RED, GRAY, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MINIMAL, FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID >UNREMARKABLE FILLED CYST, 3 MM IN DIAMETER DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, INGUINAL (IN) : LN, INGUINAL (IN) : -LARGE; LEFT: UP TO 6 X 5 X 4 MM -N-MAMMARY CARCINOMA,-NEOPLASM PRESENT (RIGHT NOT IDENTIFIED); SOME APPEAR FIRM (CAP 19) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DRY, RED MATERIAL -------------------------------------------- 4526 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 358 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92337 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/26/99 STUDY DAY OF DEATH: 569 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 410.3 GRAMS DATE AND TIME OF NECROPSY: 10/26/99 14:35 PROSECTOR: JOHN S. HALFORD RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4527 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 359 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92338 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 302.4 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 14:20 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; VENTRAL- KIDNEY (KD) : ABDOMINAL; VERIFIED AT NECROPSY: -CAST, PROTEINACEOUS,-MINIMAL, MULTI- OBSERVED FOCAL >SKIN & PELAGE; RED HAIR COAT; VENTRAL- -MINERALIZATION, PELVIC,-MINIMAL, FOCAL CERVICAL; VERIFIED AT NECROPSY: THYROID (TY) : OBSERVED -B-ADENOMA, C-CELL,-NEOPLASM PRESENT >EXCRETION; FEW FECES; VERIFIED AT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : NECROPSY: NOT APPLICABLE -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT SINGLE MASS, 12 X 14 X 25 MM, FIRM, -B-FIBROADENOMA,-NEOPLASM PRESENT NECROPSY: NOT APPLICABLE LOBULATED, AND TAN; SAME ON CUT >BEHAVIOR; ATAXIC; VERIFIED AT SURFACE (CAP 18); REGIONAL LN NECROPSY: NOT APPLICABLE (INGUINAL) NOT IDENTIFIED BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 8 X 13 MM, -CYST,-PRESENT SOFT, RED, AND DARK RED -HEMORRHAGE,-SEVERE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 4528 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 360 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92338 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 302.4 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 14:20 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DRY RED MATERIAL -------------------------------------------- 4529 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 361 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92338 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 302.4 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 14:20 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4530 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 362 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92339 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/17/00 10:40 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; KIDNEY (KD) : WEEK 66; 90 X 80MM; VERIFIED AT -MINERALIZATION, PELVIC,-SLIGHT, MULTI- NECROPSY: OBSERVED FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 111 X 80 X 35 MM (153.0 -PIGMENT,-MODERATE, FOCAL GRAMS), RED, TAN, LOBULATED, VASCULAR, -B-FIBROADENOMA,-NEOPLASM PRESENT AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL RIGHT) NOTED IN CLINICAL OBSERVATIONS HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -CYST,-PRESENT 4531 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 363 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92339 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/17/00 10:40 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); DIFFUSE: -HEMORRHAGE,-SLIGHT, MULTI-FOCAL MULTIPLE BROWN AREAS, UP TO 2 X 1 MM -PIGMENT,-MINIMAL, FOCAL -LARGE; 7 X 7 X 3 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC DEATH COMMENT (DC) : -MAMMARY FIBROADENOMA,-PRESENT -------------------------------------------- 4532 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 364 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92339 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/17/00 10:40 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4533 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 365 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92340 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 548 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 488.1 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 15:40 PROSECTOR: JILL PAUS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK KIDNEY (KD) : KIDNEY (KD) : 44; 100 X 85MM; VERIFIED AT NECROPSY: -DIFFUSELY DARK; BILATERAL: BROWN -MINERALIZATION, PELVIC,-SLIGHT, MULTI- NOT OBSERVED FOCAL >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK -PYELONEPHRITIS,-SLIGHT, FOCAL 74; 35 X 20MM; VERIFIED AT NECROPSY: LIVER (LI) : OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- >MASS(ES); VENTRAL-ABDOMINAL; WEEK 74; FOCAL 35 X 30MM; VERIFIED AT NECROPSY: THYROID (TY) : OBSERVED -MINERALIZATION,-MINIMAL, FOCAL >SKIN & PELAGE; BLACK SKIN; VENTRAL- MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : ABDOMINAL; VERIFIED AT NECROPSY: NOT -MASS(ES); LEFT AXILLARY REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL OBSERVED SINGLE MASS, 40 X 30 X 20 MM, RED, -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT >SKIN & PELAGE; BLACK SKIN; VENTRAL- DARK RED, TAN, AND SOFT; ON SECTION THORACIC-LEFT; VERIFIED AT NECROPSY: EXUDES A BROWN SEMIFLUID (CAP 20); NOT OBSERVED REGIONAL LN (SEE AXILLARY) >MASS(ES); INGUINAL REGION-LEFT; WEEK -MASS #2; LEFT INGUINAL REGION: SINGLE 79; 30 X 35MM; VERIFIED AT NECROPSY: MASS, 90 X 75 X 45 MM (144.2 GRAMS), OBSERVED RED, DARK RED, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE (CAP 18,21); REGIONAL LN (INGUINAL) NOT IDENTIFIED 4534 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 366 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92340 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 548 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 488.1 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 15:40 PROSECTOR: JILL PAUS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #3; VENTRAL ABDOMINAL REGION: SINGLE MASS, 60 X 40 X 20 MM, RED, DARK RED, TAN, AND SOFT; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A BROWN SEMIFLUID (CAP 22); REGIONAL LN (SEE AXILLARY) BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LIGHT FOCUS (I)/AREA(S); BILATERAL: -DEGENERATION, CYSTIC, CORTICAL,- MULTIPLE TAN PINPOINT FOCI MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 7 X 7 MM, RED, DARK -HEMORRHAGE,-MODERATELY SEVERE, MULTI- RED, TAN, AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; LEFT: 13 X 7 X 4 MM (RIGHT IS >UNREMARKABLE 4 X 3 X 2 MM) (CAP 19) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED AND DRY -ANIMAL THIN 4535 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 367 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92340 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 548 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 488.1 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 15:40 PROSECTOR: JILL PAUS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4536 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 368 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92341 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 505.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:53 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0350 .0069 % .0160 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0283 .0056 % .0130 WEIGHT TAKEN BRAIN (BR) 2.1849 .4327 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.7424 .3450 % .7975 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.8779 .3719 % .8595 WEIGHT TAKEN LIVER (LI) 13.3619 2.6459 % 6.1156 WEIGHT TAKEN LUNG (LU) 1.5514 .3072 % .7101 WEIGHT TAKEN LEFT OVARY (OV0) .0415 .0082 % .0190 WEIGHT TAKEN RIGHT OVARY (OV1) .0362 .0072 % .0166 WEIGHT TAKEN SPLEEN (SP) .5689 .1127 % .2604 WEIGHT TAKEN LF THYROID/PARA (TT0) .0139 .0028 % .0064 WEIGHT TAKEN RT THYROID/PARA (TT1) .0147 .0029 % .0067 WEIGHT TAKEN UTERUS (UT) .9924 .1965 % .4542 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; KIDNEY (KD) : WEEK 53; 10 X 10MM; VERIFIED AT -CYST,-SLIGHT, MULTI-FOCAL NECROPSY: NOT OBSERVED -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); VENTRAL ABDOMINAL REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 12 MM IN DIAMETER (CAP 19) ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL 4537 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 369 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92341 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 505.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:53 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); DIFFUSE: -HEMORRHAGE,-SLIGHT, FOCAL MULTIPLE DARK RED AREAS, UP TO 1 MM IN -PIGMENT,-MINIMAL, FOCAL DIAMETER -B-ADENOMA,-NEOPLASM PRESENT CECUM (CE) : -EDEMA,-SLIGHT, MULTI-FOCAL UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI- FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; -HEMORRHAGE,-MINIMAL, MULTI-FOCAL COLLECTED GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 4538 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 370 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92341 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 505.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:53 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4539 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 371 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92342 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/15/99 STUDY DAY OF DEATH: 344 STUDY WEEK OF DEATH: 50 TERMINAL BODY WEIGHT: 268.1 GRAMS DATE AND TIME OF NECROPSY: 03/15/99 11:31 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LUNG (LU) : NECROPSY: OBSERVED -INFILTRATE, MACROPHAGE, ALVEOLAR,- >SKIN & PELAGE; YELLOW HAIR COAT; MINIMAL, MULTI-FOCAL VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : >APPEARANCE; THIN; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT OBSERVED MINIMAL, MULTI-FOCAL >RESPIRATION; AUDIBLE; VERIFIED AT THYROID (TY) : NECROPSY: NOT APPLICABLE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >EXCRETION; FEW FECES; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); LEFT POSTERIOR -HEMORRHAGE,-MODERATE, MULTI-FOCAL ASPECT: SINGLE DARK BROWN AREA, 3 MM -B-ADENOMA,-NEOPLASM PRESENT IN DIAMETER DEATH COMMENT (DC) : -FRACTURED CALVARIUM,-PRESENT CALVARIUM/SKULL (CA) : CALVARIUM/SKULL (CA) : -FRACTURE; AT FIRST RUGAE: SURROUNDING -CYST, KERATIN,-PRESENT SOFT TISSUE IS DARK RED AND -FRACTURE,-PRESENT GELATINOUS; COLLECTED -HEMORRHAGE,-MODERATELY SEVERE, MULTI- FOCAL 4540 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 372 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92342 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/15/99 STUDY DAY OF DEATH: 344 STUDY WEEK OF DEATH: 50 TERMINAL BODY WEIGHT: 268.1 GRAMS DATE AND TIME OF NECROPSY: 03/15/99 11:31 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CALVARIUM/SKULL (CA) : -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -STAINS-PERINEUM/PERIANAL; YELLOW, MOIST MATERIAL -GI TRACT-LARGE LUMEN; ENTIRE GI TRACT: DISTENDED WITH GAS -DISCHARGE-OCULAR; BILATERAL: RED, DRY MATERIAL -DISCHARGE-NASAL; RED, DRY MATERIAL -------------------------------------------- 4541 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 373 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92342 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/15/99 STUDY DAY OF DEATH: 344 STUDY WEEK OF DEATH: 50 TERMINAL BODY WEIGHT: 268.1 GRAMS DATE AND TIME OF NECROPSY: 03/15/99 11:31 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4542 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 374 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92343 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/10/99 STUDY DAY OF DEATH: 400 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 398.0 GRAMS DATE AND TIME OF NECROPSY: 05/10/99 14:21 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); SHOULDER-RIGHT; WEEK 19; 65 KIDNEY (KD) : X 60MM; VERIFIED AT NECROPSY: OBSERVED -HYPERPLASIA, TRANSITIONAL CELL,- >SKIN & PELAGE; SORE/SCAB; SHOULDER- SLIGHT, MULTI-FOCAL RIGHT; VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-SLIGHT, FOCAL -PYELONEPHRITIS,-MINIMAL, MULTI-FOCAL LIVER (LI) : LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL SKIN (SK) : -EROSION,-MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL SINGLE MASS, 80 X 50 X 25 MM, RED, -NECROSIS,-MODERATE, FOCAL TAN, FIRM, AND LOBULATED; SAME ON CUT -B-FIBROADENOMA,-NEOPLASM PRESENT SURFACE; COLLECTED; OVERLYING SKIN HAS A MOIST, RED, ULCERATED AREA, 25 MM IN DIAMETER; COLLECTED INTACT WITH MASS; REGIONAL LN (AXILLARY) NORMAL (CAP 18) SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, MULTI-FOCAL 4543 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 375 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92343 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/10/99 STUDY DAY OF DEATH: 400 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 398.0 GRAMS DATE AND TIME OF NECROPSY: 05/10/99 14:21 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT ASPECT: -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SINGLE DARK RED FOCUS, 1 MM IN -B-ADENOMA,-NEOPLASM PRESENT DIAMETER DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -GI TRACT-DARK FLUID CONTENT; ENTIRE GI TRACT: DARK BROWN, TENACIOUS SEMIFLUID -------------------------------------------- 4544 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 376 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92343 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/10/99 STUDY DAY OF DEATH: 400 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 398.0 GRAMS DATE AND TIME OF NECROPSY: 05/10/99 14:21 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4545 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 377 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92344 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 375.3 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 8:40 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LUNG (LU) : LUNG (LU) : NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); TRIMMING -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- >BEHAVIOR; ATAXIC; VERIFIED AT COMMENT: ALONG CAUDAL MARGIN OF LEFT FOCAL NECROPSY: NOT APPLICABLE LOBE: SINGLE TAN AREA, 3 MM IN -INFILTRATE, MACROPHAGE, ALVEOLAR,- >APPEARANCE; HUNCHED; VERIFIED AT DIAMETER; SUBMITTED IN BLOCK 22 SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS: DIFFUSE; -GALACTOCELE,-SLIGHT, MULTI-FOCAL COLLECTED ON ROUTINE SECTION BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 8 X 7 MM, DARK RED AND -HEMORRHAGE,-MODERATELY SEVERE, MULTI- SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL 4546 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 378 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92344 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 375.3 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 8:40 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ILEUM (IL) : ILEUM (IL) : -LARGE LYMPHOID AREA(S); DIFFUSE: UP >UNREMARKABLE TO 5 X 2 MM; COLLECTED ON ROUTINE SECTION URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MODERATELY SEVERE, FOCAL VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: -INFLAMMATION, ACUTE,-MODERATELY CONTAINS A LIGHT GREEN SEMIFLUID SEVERE, FOCAL MATERIAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: LOWER INCISORS -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY MATERIAL -------------------------------------------- 4547 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 379 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92344 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 375.3 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 8:40 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), OVARY (OV), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4548 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 380 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92345 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/28/98 STUDY DAY OF DEATH: 206 STUDY WEEK OF DEATH: 30 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/28/98 14:06 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-PRESENT -LIGHT FOCUS(I)/AREA(S); LEFT: -MINERALIZATION, PELVIC,-MODERATE, MULTIPLE TAN AREAS, UP TO 10 X 7 MM MULTI-FOCAL -MINERALIZATION, TUBULAR,-MODERATE, MULTI-FOCAL -PYELONEPHRITIS,-SEVERE, MULTI-FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL CERVIX (CV) : CERVIX (CV) : -LARGE; 10 MM IN DIAMETER >UNREMARKABLE DEATH COMMENT (DC) : -PYELONEPHRITIS,-PRESENT -------------------------------------------- 4549 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 381 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92345 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/28/98 STUDY DAY OF DEATH: 206 STUDY WEEK OF DEATH: 30 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/28/98 14:06 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4550 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 382 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92346 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/14/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 247.5 GRAMS DATE AND TIME OF NECROPSY: 06/14/99 14:35 PROSECTOR: TODD ACKER RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); NOSE; WEEK 46; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, FOCAL >SKIN & PELAGE; ROUGH HAIR COAT; PARATHYROID (PT) : VERIFIED AT NECROPSY: NOT APPLICABLE -FIBROSIS,-SLIGHT, MULTI-FOCAL >APPEARANCE; HUNCHED; VERIFIED AT SKIN (SK) : SKIN (SK) : NECROPSY: NOT APPLICABLE -CRUSTED AREA(S); TIP OF NOSE: SINGLE -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM >APPEARANCE; THIN; VERIFIED AT BROWN, DRY AND RAISED AREA, 5 X 3 X 3 PRESENT NECROPSY: OBSERVED MM (CAP 18) >BEHAVIOR; ATAXIC; VERIFIED AT MAMMARY, FEMALE (MF) : NECROPSY: NOT APPLICABLE -GALACTOCELE,-SLIGHT, MULTI-FOCAL >EYES; RED DISCHARGE; EYES; VERIFIED AT -MINERALIZATION,-MINIMAL, MULTI-FOCAL NECROPSY: OBSERVED BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 7 MM IN DIAMETER (FOR -ANGIECTASIS,-SLIGHT, MULTI-FOCAL COMPARISON, LEFT IS 4 MM IN DIAMETER) -DEGENERATION, CYSTIC, CORTICAL,- MODERATELY SEVERE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 13 X 9 X 6 MM, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- DARK RED, BROWN AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4551 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 383 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92346 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/14/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 247.5 GRAMS DATE AND TIME OF NECROPSY: 06/14/99 14:35 PROSECTOR: TODD ACKER RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4552 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 384 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92347 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/01/99 STUDY DAY OF DEATH: 330 STUDY WEEK OF DEATH: 48 TERMINAL BODY WEIGHT: 209.8 GRAMS DATE AND TIME OF NECROPSY: 03/01/99 14:26 PROSECTOR: TERRI L. WILLIAMS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; YELLOW HAIR COAT; KIDNEY (KD) : VERIFIED AT NECROPSY: NOT OBSERVED -CYST,-MODERATE, FOCAL >APPEARANCE; THIN; VERIFIED AT MAMMARY, FEMALE (MF) : NECROPSY: NOT OBSERVED -GALACTOCELE,-SLIGHT, MULTI-FOCAL >RESPIRATION; LABORED; VERIFIED AT BONE, STERNUM (SB) : BONE, STERNUM (SB) : NECROPSY: NOT APPLICABLE -MISALIGNED; MANUBRIUM: EXTENDS INTO >UNREMARKABLE THORACIC CAVITY; COLLECTED INTACT WITH RIBCAGE BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 11 X 5 MM; DARK RED, >TISSUE MISSING GRAY TAN AND SOFT; EXTENDS UNDER LEFT TRIGEMINAL NERVE OVARY (OV) : OVARY (OV) : -DIFFUSELY RED; BILATERAL: DARK RED >UNREMARKABLE DEATH COMMENT (DC) : -OSTEOMA,-PRESENT NASAL TURBINATE (NT) : -B-OSTEOMA,-NEOPLASM PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL 4553 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 385 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92347 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/01/99 STUDY DAY OF DEATH: 330 STUDY WEEK OF DEATH: 48 TERMINAL BODY WEIGHT: 209.8 GRAMS DATE AND TIME OF NECROPSY: 03/01/99 14:26 PROSECTOR: TERRI L. WILLIAMS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -GI TRACT-LIGHT FLUID CONTENT; STOMACH: YELLOW SEMIFLUID -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4554 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 386 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92348 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 422.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:49 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, MULTI- >MASS(ES); AXILLARY REGION-RIGHT; WEEK FOCAL 101; 50 X 45MM; VERIFIED AT NECROPSY: PANCREAS (PA) : OBSERVED -HEMORRHAGE,-MODERATE, MULTI-FOCAL >SKIN & PELAGE; SORE/SCAB; PAW-HIND- -B-ADENOMA, ISLET CELL,-NEOPLASM RIGHT; VERIFIED AT NECROPSY: OBSERVED PRESENT >MASS(ES); VENTRAL-ABDOMINAL; WEEK 100; THYROID (TY) : THYROID (TY) : 60 X 40MM; VERIFIED AT NECROPSY: >NOTE:>THYROID/ PARATHYROID TAKEN OFF -CYST, ULTIMOBRANCHIAL,-PRESENT OBSERVED TRACHEA/ ESOPHAGUS AT NECROPSY PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT, DIFFUSE MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-MODERATELY SEVERE, MULTI- SINGLE MASS, 60 X 48 X 33 MM, TAN, FOCAL RED, AND FIRM; ON SECTION, EXUDES A -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT WHITE CREAMY SEMIFLUID (CAP 24); REGIONAL LN (SEE INGUINAL) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTIONS 4555 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 387 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92348 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 422.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:49 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 54 X 40 X 25 MM, TAN, RED, FIRM AND LOBULATED; ON CUT SURFACE, EXUDES A TAN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (SEE INGUINAL) EYE (EY) : -FIBROSIS, CORNEAL,-SLIGHT, FOCAL -INFLAMMATION, ACUTE, CORNEAL,-MINIMAL, FOCAL -ULCERATION, CORNEAL,-MINIMAL, FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE LN, MESENTERIC (MS) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL THYMUS (TH) : THYMUS (TH) : -MASS(ES); LEFT LOBE: SINGLE MASS, 7 X -M-THYMIC CARCINOMA,-NEOPLASM PRESENT 6 X 5 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE MEDIASTINAL) ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -THROMBUS,-SLIGHT, MULTI-FOCAL 4556 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 388 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92348 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 422.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:49 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 5 X 3 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, GRAY AND FIRM -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL RECTUM (RE) : -PARASITISM,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN (CAP -HYPERPLASIA, LYMPHOCYTIC,-MODERATELY 22) SEVERE, DIFFUSE -LARGE; BILATERAL: EACH IS UP TO 10 X 6 X 2 MM FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HIND FOOT: -EROSION,-MODERATELY SEVERE, FOCAL SINGLE RED, TAN, MOIST CRUSTED AREA, -FIBROSIS,-MODERATELY SEVERE, MULTI- 12 X 9 MM; COLLECTED FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL LN, INGUINAL (IN) : LN, INGUINAL (IN) : -LARGE; LEFT: 10 X 6 X 3 MM (CAP 25) -HYPERPLASIA, LYMPHOCYTIC,-MODERATELY (RIGHT NOT IDENTIFIED) SEVERE, DIFFUSE LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -MOTTLED; RED AND TAN (CAP 23) -HEMORRHAGE,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 4557 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 389 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92348 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 422.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:49 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, LOWER INCISORS -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4558 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 390 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92349 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/28/99 STUDY DAY OF DEATH: 541 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/28/99 14:00 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-SLIGHT, FOCAL -MINERALIZATION, PELVIC,-SLIGHT, FOCAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT THYMUS (TH) : -CYST,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 9 X 8 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, DARK RED, TAN, GRAY AND SOFT -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL: UPPER INCISORS -------------------------------------------- 4559 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 391 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92349 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/28/99 STUDY DAY OF DEATH: 541 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/28/99 14:00 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4560 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 392 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92350 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 325.5 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 14:09 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYES; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >SKIN & PELAGE; PALE; ENTIRE BODY; FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-SLIGHT, MULTI- >RESPIRATION; AUDIBLE; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE MAMMARY, FEMALE (MF) : >RESPIRATION; LABORED; VERIFIED AT -GALACTOCELE,-SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE BRAIN (BR) : BRAIN (BR) : >APPEARANCE; HEAD TILT; RIGHT; VERIFIED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE AT NECROPSY: NOT APPLICABLE ADRENAL, CORTEX (AC) : >DISCHARGE; NASAL; RED IN COLOR; -ANGIECTASIS,-MODERATE, FOCAL VERIFIED AT NECROPSY: OBSERVED -HEMORRHAGE,-MINIMAL, FOCAL >DISCHARGE; OTHER; RED ORAL; VERIFIED -THROMBUS,-MINIMAL, FOCAL AT NECROPSY: OBSERVED PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 7 X 4 MM, DARK RED AND -HEMORRHAGE,-MODERATE, MULTI-FOCAL SOFT -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -DISCHARGE-NASAL; DRY RED MATERIAL -DISCHARGE-ORAL; MOIST RED MATERIAL 4561 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 393 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92350 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 325.5 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 14:09 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4562 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 394 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92351 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 417.8 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:40 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LUNG (LU) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -DARK FOCUS(I)/AREA(S); LEFT: SINGLE -ANGIECTASIS,-MODERATE, MULTI-FOCAL DARK BROWN FOCUS, 2 MM IN DIAMETER -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -THROMBUS,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -RAISED AREA(S); RIGHT ANTERIOR -HEMORRHAGE,-MINIMAL, MULTI-FOCAL ASPECT: SINGLE TAN RAISED AREA, UP TO -B-ADENOMA,-NEOPLASM PRESENT 1 MM IN DIAMETER HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4563 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 395 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92351 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 417.8 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:40 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4564 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 396 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92352 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 215.4 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:59 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.8244 3.6325 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : UNVERIFIED AT NECROPSY -NO MACROSCOPIC LESIONS -------------------------------------------- 4565 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 397 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92352 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 215.4 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:59 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4566 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 398 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92353 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 323.0 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 13:44 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >BEHAVIOR; HYPOACTIVE; VERIFIED AT LUNG (LU) : NECROPSY: NOT APPLICABLE -MINERALIZATION, VASCULAR,-SLIGHT, >EXCRETION; FEW FECES; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >DISCHARGE; NASAL; RED IN COLOR; -MINERALIZATION, PELVIC,-MODERATE, VERIFIED AT NECROPSY: OBSERVED MULTI-FOCAL >SKIN & PELAGE; BROWN HAIR COAT; -NEPHROPATHY, CHRONIC PROGRESSIVE,- VENTRAL-ABDOMINAL; VERIFIED AT MINIMAL, MULTI-FOCAL NECROPSY: NOT OBSERVED LIVER (LI) : >BEHAVIOR; ATAXIC; MODERATE; VERIFIED -VACUOLATION, HEPATOCELLULAR,-SLIGHT, AT NECROPSY: NOT APPLICABLE MULTI-FOCAL >APPEARANCE; HEAD TILT; LEFT; VERIFIED THYROID (TY) : AT NECROPSY: NOT APPLICABLE -CYST, ULTIMOBRANCHIAL,-PRESENT >RESPIRATION; LABORED; VERIFIED AT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : NECROPSY: NOT APPLICABLE -CYST(S); ALL REGIONS, DIFFUSE: -GALACTOCELE,-MODERATELY SEVERE, MULTI- >SKIN & PELAGE; WARM TO TOUCH; ENTIRE MULTIPLE TAN CREAMY FLUID FILLED FOCAL BODY; VERIFIED AT NECROPSY: NOT CYSTS, UP TO 6 MM IN DIAMETER (CAP 18) -MINERALIZATION,-MINIMAL, MULTI-FOCAL APPLICABLE -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL 4567 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 399 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92353 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 323.0 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 13:44 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -HYPERTROPHY,-MODERATE, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 6 MM, RED, DARK -CYST,-PRESENT RED, GREY AND SOFT -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; YELLOW -DISCHARGE-OCULAR; LEFT: RED -DISCHARGE-NASAL; BILATERAL: RED -------------------------------------------- 4568 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 400 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92353 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 323.0 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 13:44 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4569 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 401 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92354 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 385.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:11 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; AXILLARY LUNG (LU) : REGION-RIGHT; VERIFIED AT NECROPSY: -MINERALIZATION, VASCULAR,-MINIMAL, OBSERVED FOCAL >SKIN & PELAGE; RED HAIR COAT; HEAD- KIDNEY (KD) : ENTIRE; VERIFIED AT NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >BEHAVIOR; HYPOACTIVE; VERIFIED AT MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >APPEARANCE; HEAD TILT; LEFT; VERIFIED -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -FOCUS, ALTERED HEPATOCELLULAR, AT NECROPSY: NOT APPLICABLE MULTIPLE TAN AND WHITE AREAS, UP TO 6 EOSINOPHILIC,-MODERATE, MULTI-FOCAL MM IN DIAMETER; SOME ARE SLIGHTLY -VACUOLATION, HEPATOCELLULAR,-SLIGHT, RAISED; EXTRA SECTION (CAP 22) MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,- 3 NEOPLASMS PRESENT PANCREAS (PA) : PANCREAS (PA) : -MASS(ES); ALONG DUODENUM: SINGLE -HEMORRHAGE,-SLIGHT, MULTI-FOCAL MASS, 7 X 9 X 13 MM, FIRM, LOBULATED, -B-ADENOMA, ISLET CELL,- 3 NEOPLASMS RED, DARK RED AND TAN; SAME ON CUT PRESENT SURFACE (CAP 24); REGIONAL LN (PANCREATIC) (CAP 25) SKIN (SK) : SKIN (SK) : -ABRASION(S)/ULCERATION(S); RIGHT >UNREMARKABLE AXILLARY REGION: SINGLE DARK RED AND BROWN ABRADED AREA, 8 MM IN DIAMETER (CAP 23) 4570 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 402 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92354 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 385.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:11 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT HEART (HT) : -METAPLASIA, CARTILAGE,-MODERATE, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 8 X 11 MM, -B-ADENOMA,-NEOPLASM PRESENT FIRM, RED, DARK RED AND TAN HARDERIAN GLAND (HG) : -HYPERPLASIA, GLANDULAR,-MODERATE, FOCAL UTERUS (UT) : -DILATATION,-MODERATE, FOCAL -B-POLYP, ENDOMETRIAL, STROMAL,- NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -STAINS - SKIN/HAIRCOAT; HEAD AND ENTIRE VENTRAL SURFACE: DRY, RED MATERIAL 4571 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 403 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92354 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 385.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:11 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), THYROID (TY), PARATHYROID (PT), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, ANT MES/PANC (AP) ------------------------------------------------------------------------------------------------------------------------------------ 4572 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 404 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92355 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 192.3 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 9:00 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.0644 3.6736 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : UNVERIFIED AT NECROPSY -NO MACROSCOPIC LESIONS -------------------------------------------- 4573 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 405 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92355 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 192.3 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 9:00 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4574 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 406 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92356 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 368.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:31 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0453 .0123 % .0202 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0347 .0094 % .0155 WEIGHT TAKEN BRAIN (BR) 2.2412 .6090 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.3414 .3645 % .5985 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.3549 .3682 % .6045 WEIGHT TAKEN LIVER (LI) 10.1005 2.7447 % 4.5067 WEIGHT TAKEN LUNG (LU) 1.4449 .3926 % .6447 WEIGHT TAKEN LEFT OVARY (OV0) .0361 .0098 % .0161 WEIGHT TAKEN RIGHT OVARY (OV1) .0422 .0115 % .0188 WEIGHT TAKEN SPLEEN (SP) .5418 .1472 % .2417 WEIGHT TAKEN LF THYROID/PARA (TT0) .0110 .0030 % .0049 WEIGHT TAKEN RT THYROID/PARA (TT1) .0140 .0038 % .0062 WEIGHT TAKEN UTERUS (UT) .8728 .2372 % .3894 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); BILATERAL INGUINAL REGIONS: -GALACTOCELE,-MODERATELY SEVERE, MULTI- MULTIPLE TAN CREAMY SEMIFLUID FILLED FOCAL CYSTS, UP TO 6 MM IN DIAMETER; -MINERALIZATION,-MINIMAL, MULTI-FOCAL COLLECTED ON ROUTINE SECTION ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL 4575 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 407 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92356 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 368.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:31 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); LEFT ASPECT: -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SINGLE BLACK AREA, 3 X 1 MM -B-ADENOMA,-NEOPLASM PRESENT -LARGE; 7 X 6 X 4 MM; APPEARS SWOLLEN DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL UPPER INCISORS -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: BROKEN 3 MM FORM BASE -------------------------------------------- 4576 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 408 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92356 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 368.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:31 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4577 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 409 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92357 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 605.8 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:47 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0527 .0087 % .0251 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0429 .0071 % .0204 WEIGHT TAKEN BRAIN (BR) 2.1032 .3472 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.7928 .2959 % .8524 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.9039 .3143 % .9052 WEIGHT TAKEN LIVER (LI) 17.4056 2.8732 % 8.2758 WEIGHT TAKEN LUNG (LU) 1.4364 .2371 % .6830 WEIGHT TAKEN LEFT OVARY (OV0) .0462 .0076 % .0220 WEIGHT TAKEN RIGHT OVARY (OV1) .0343 .0057 % .0163 WEIGHT TAKEN SPLEEN (SP) .8142 .1344 % .3871 WEIGHT TAKEN LF THYROID/PARA (TT0) .0212 .0035 % .0101 WEIGHT TAKEN RT THYROID/PARA (TT1) .0180 .0030 % .0086 WEIGHT TAKEN UTERUS (UT) 1.3092 .2161 % .6225 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-SLIGHT, MULTI- FOCAL -MINERALIZATION, PELVIC,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT 4578 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 410 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92357 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 605.8 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:47 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE >UNREMARKABLE BLACK FOCUS, 3 MM IN DIAMETER UTERUS (UT) : -DILATATION,-SLIGHT, FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL; COLLECTED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 4579 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 411 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92357 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 605.8 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:47 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4580 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 412 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92358 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 351.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:40 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK KIDNEY (KD) : 84; 20 X 20MM; VERIFIED AT NECROPSY: -CAST, PROTEINACEOUS,-MINIMAL, MULTI- OBSERVED FOCAL >SKIN & PELAGE; RED HAIR COAT; HEAD- PANCREAS (PA) : ENTIRE; VERIFIED AT NECROPSY: OBSERVED -ATROPHY, ACINAR,-MODERATE, MULTI-FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY: SINGLE -GALACTOCELE,-MODERATELY SEVERE, MULTI- MASS, 20 X 17 X 15 MM, DARK RED, TAN, FOCAL AND SOFT; CUT SURFACE CONTAINS A TAN -HEMORRHAGE,-MINIMAL, MULTI-FOCAL FLUID; COLLECTED; REGIONAL LN -B-FIBROADENOMA,-NEOPLASM PRESENT (AXILLARY) NORMAL (CAP 22) -M-CARCINOMA,-NEOPLASM PRESENT BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -THROMBUS,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 11 X 8 MM, DARK RED, -HEMORRHAGE,-MINIMAL, MULTI-FOCAL TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT 4581 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 413 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92358 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 351.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:40 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: DARK RED AND DRY -------------------------------------------- 4582 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 414 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92358 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 351.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:40 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4583 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 415 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92359 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/13/00 STUDY DAY OF DEATH: 648 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/13/00 8:23 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK KIDNEY (KD) : 79; 30 X 30MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MINIMAL, MULTI- OBSERVED FOCAL >MASS(ES); AXILLARY REGION-LEFT; WEEK LIVER (LI) : 92; 10 X 10MM; VERIFIED AT NECROPSY: -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- OBSERVED FOCAL >EXCRETION; FEW FECES; VERIFIED AT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : NECROPSY: NOT APPLICABLE -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL >APPEARANCE; THIN; VERIFIED AT SINGLE MASS, 40 X 32 X 25 MM, DARK -B-FIBROADENOMA,- 3 NEOPLASMS PRESENT NECROPSY: OBSERVED RED, TAN, GRAY, AND FIRM; SAME ON CUT >EYES; RED DISCHARGE; EYES; VERIFIED AT SURFACE; COLLECTED; REGIONAL LN NECROPSY: OBSERVED (AXILLARY) NORMAL (CAP 18) -CYST(S); BILATERAL PECTORAL REGIONS AND PERINEAL REGION: MULTIPLE TAN AND CREAMY SEMIFLUID FILLED CYSTS, UP TO 15 MM IN DIAMETER (CAP 19) -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 20 X 19 X 10 MM, DARK RED, TAN, GRAY, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 18) BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT 4584 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 416 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92359 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/13/00 STUDY DAY OF DEATH: 648 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/13/00 8:23 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL THYMUS (TH) : -CYST,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 8 X 6 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL TAN, AND SOFT -PIGMENT,-SLIGHT, FOCAL -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC UTERUS (UT) : UTERUS (UT) : -CYST(S); LEFT, WITHIN WALL: SINGLE -DILATATION,-MODERATE, FOCAL CLEAR FLUID FILLED CYST, 8 X 5 X 5 MM OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID >UNREMARKABLE FILLED CYST, 7 MM IN DIAMETER DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, MEDIASTINAL (ML) : -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT, MULTI-FOCAL 4585 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 417 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92359 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/13/00 STUDY DAY OF DEATH: 648 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/13/00 8:23 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MEDIASTINAL (ML) : >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4586 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 418 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92360 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 585.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:24 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK KIDNEY (KD) : 61; 80 X 65MM; VERIFIED AT NECROPSY: -CAST, PROTEINACEOUS,-MINIMAL, MULTI- OBSERVED FOCAL >MASS(ES); AXILLARY REGION-RIGHT; WEEK LIVER (LI) : LIVER (LI) : 92; 45 X 45MM; VERIFIED AT NECROPSY: -RED FOCUS(I)/AREA(S); ALL LOBES: -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- OBSERVED MULTIPLE RED AREAS, UP TO 3 X 2 MM; FOCAL >MASS(ES); AXILLARY REGION-LEFT; WEEK COLLECTED ON ROUTINE SECTIONS -TELANGIECTASIS,-MINIMAL, FOCAL 96; 60 X 60MM; VERIFIED AT NECROPSY: SKIN (SK) : OBSERVED >NOTE:>SKIN TAKEN FROM RIGHT SIDE DUE >SKIN & PELAGE; BLUE SKIN; VENTRAL- TO MASS ON LEFT SIDE ABDOMINAL-LEFT; VERIFIED AT NECROPSY: MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : NOT APPLICABLE -MASS(ES); LEFT INGUINAL REGION, -FIBROSIS,-SEVERE, FOCAL >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; EXTENDS TO MID PERIANAL REGION: SINGLE -GALACTOCELE,-SEVERE, MULTI-FOCAL VERIFIED AT NECROPSY: OBSERVED MASS, 90 X 60 X 20 MM, RED, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL TAN AND SOFT; SAME ON CUT SURFACE; -MINERALIZATION,-SLIGHT, MULTI-FOCAL SOME AREAS EXUDE A REDDISH-BROWN -B-FIBROADENOMA,-NEOPLASM PRESENT SEMIFLUID; COLLECTED; REGIONAL LN -M-CARCINOMA,- 2 NEOPLASMS PRESENT (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 50 X 40 X 35 MM, RED, DARK RED, TAN AND SOFT; SOME SECTIONS EXUDE A REDDISH-BROWN SEMIFLUID (CAP 23); REGIONAL LN (SEE AXILLARY) 4587 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 419 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92360 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 585.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:24 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #3; RIGHT AXILLARY REGION: SINGLE MASS, 50 X 40 X 25 MM, RED, TAN AND SOFT; ON SECTION, EXUDES A TAN CREAMY SEMIFLUID (CAP 25); REGIONAL LN (SEE AXILLARY) >NOTE:>MAMMARY TAKEN FROM RIGHT SIDE DUE TO MASS ON LEFT SIDE BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT THYMUS (TH) : -CYST,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 3 MM, RED, DARK -HEMORRHAGE,-SLIGHT, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT UTERUS (UT) : UTERUS (UT) : -CYST(S); RIGHT, WITHIN HORN: SINGLE -DILATATION,-MODERATELY SEVERE, FOCAL CLEAR FLUID FILLED CYST, 10 X 5 X 5 MM OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 2 MM IN DIAMETER 4588 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 420 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92360 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 585.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:24 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; BILATERAL: LEFT IS 12 X 6 X 3 >UNREMARKABLE MM, RIGHT IS 10 X 5 X 2 MM (CAP 24) -MOTTLED; LEFT: RED AND TAN FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); BILATERAL HINDFEET: -EROSION,-SLIGHT, FOCAL SINGLE TAN, DRY CRUSTED AREA ON EACH, -FIBROSIS,-MODERATELY SEVERE, MULTI- EACH IS 3 MM IN DIAMETER; COLLECTED FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4589 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 421 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92360 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 585.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:24 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4590 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 422 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92361 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/07/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 664.1 GRAMS DATE AND TIME OF NECROPSY: 03/07/00 13:36 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK LUNG (LU) : 73; 110 X 100MM; VERIFIED AT NECROPSY: -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL OBSERVED -INFILTRATE, MACROPHAGE, ALVEOLAR,- >MASS(ES); AXILLARY REGION-LEFT; WEEK MINIMAL, FOCAL 80; 40 X 35MM; VERIFIED AT NECROPSY: KIDNEY (KD) : OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, FOCAL >EYES; PALE; EYES; VERIFIED AT THYROID (TY) : NECROPSY: NOT APPLICABLE -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -GALACTOCELE,-MODERATELY SEVERE, MULTI- SINGLE MASS, 55 X 45 X 35 MM (39.6 FOCAL GRAMS), TAN, SOFT AND LOBULATED; ON -INFLAMMATION, ACUTE,-SLIGHT, FOCAL SECTION, EXUDES A TAN CREAMY SEMIFLUID -B-FIBROADENOMA,- 3 NEOPLASMS PRESENT MATERIAL; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) -THICKENED; VENTRAL THORACIC REGION: TAN AND FIRM (CAP 23) 4591 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 423 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92361 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/07/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 664.1 GRAMS DATE AND TIME OF NECROPSY: 03/07/00 13:36 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #2; LEFT VENTRAL ABDOMINAL REGION, EXTENDING TO BILATERAL INGUINAL REGIONS: SINGLE MASS, 125 X 105 X 60 MM (297.2 GRAMS), TAN, RED, FIRM, VASCULAR, AND LOBULATED; SAME ON CUT SECTION; PORTIONS COLLECTED; OVERLYING SKIN HAS A SINGLE BROWN DRY ULCERATED AREA, 30 MM IN DIAMETER (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 7 X 6 X 5 -ANGIECTASIS,-MODERATE, MULTI-FOCAL MM -HEMORRHAGE,-MODERATE, MULTI-FOCAL -MOTTLED; BILATERAL: DARK BROWN AND TAN PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 5 X 4 MM, TAN, DARK -HEMORRHAGE,-MINIMAL, MULTI-FOCAL RED, SOFT, AND LOBULATED -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -MAMMARY FIBROADENOMA,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; BILATERAL: UP TO 10 X 6 X 3 MM -HYPERPLASIA, LYMPHOCYTIC,-MINIMAL, (CAP 22) MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 4592 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 424 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92361 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/07/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 664.1 GRAMS DATE AND TIME OF NECROPSY: 03/07/00 13:36 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4593 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 425 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92362 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 321.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:45 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK KIDNEY (KD) : 102; 30 X 30MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MINIMAL, MULTI- OBSERVED FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT, MULTI-FOCAL LIVER (LI) : LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES, -FIBROSIS,-SEVERE, MULTI-FOCAL DIFFUSE: MULTIPLE TAN AREAS, UP TO 2 X -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- 1 MM; COLLECTED ON ROUTINE SECTIONS FOCAL -NECROSIS, HEPATOCELLULAR,-SEVERE, MULTI-FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-MINIMAL, DIFFUSE MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 35 X 28 X 25 MM, RED, -M-CARCINOMA,-NEOPLASM PRESENT DARK RED, TAN, AND FIRM; ON SECTION EXUDES DARK BROWN FLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION 4594 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 426 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92362 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 321.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:45 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT HEART (HT) : HEART (HT) : -LIGHT FOCUS(I)/AREA(S); AT APEX: -DEGENERATION/NECROSIS,-MINIMAL, MULTI- SINGLE LIGHT AREA, 4 MM IN DIAMETER FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -MASS(ES); RIGHT, INVOLVING ENTIRE -DEGENERATION, CYSTIC, CORTICAL,- ORGAN: SINGLE MASS, RED, DARK RED, SEVERE, MULTI-FOCAL TAN, AND FIRM, 10 X 8 X 8 MM; SAME ON -HEMORRHAGE,-MODERATE, MULTI-FOCAL CUT SURFACE; COLLECTED; REGIONAL LN -MINERALIZATION,-MODERATELY SEVERE, (RENAL) NOT IDENTIFIED FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 6 X 6 MM, RED, DARK -HEMORRHAGE,-SLIGHT, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID >UNREMARKABLE FILLED CYST, 3 MM IN DIAMETER DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4595 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 427 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92362 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 321.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:45 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4596 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 428 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92363 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 573.9 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 11:38 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK KIDNEY (KD) : 76; 80 X 80MM; VERIFIED AT NECROPSY: -CAST, PROTEINACEOUS,-MINIMAL, MULTI- OBSERVED FOCAL SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL REGION -GALACTOCELE,-MINIMAL, MULTI-FOCAL EXTENDING TO LEFT AXILLARY REGION: >NOTE:>MAMMARY MASS DESCRIBED GROSSLY SINGLE MASS, 70 X 55 X 45 MM, RED, WAS A SUBCUTANEOUS FIBROMA TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE MANDIBULAR) BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 7 X 6 MM, DARK RED, -HEMORRHAGE,-SEVERE, MULTI-FOCAL FIRM AND LOBULATED -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : UTERUS (UT) : -SMALL; BILATERAL: EACH IS 2 MM IN >UNREMARKABLE DIAMETER OVARY (OV) : >UNREMARKABLE 4597 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 429 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92363 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 573.9 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 11:38 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN (CAP -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 18) -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL LN, AXILLARY (AX) : LN, AXILLARY (AX) : -DIFFUSELY RED; LEFT: DARK RED (CAP >UNREMARKABLE 19) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DARK RED, BROWN AND DRY MATERIAL -DISCHARGE-NASAL; DARK RED AND DRY MATERIAL -------------------------------------------- 4598 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 430 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92363 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 573.9 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 11:38 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4599 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 431 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92364 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 438 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 206.9 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 15:13 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -HEMORRHAGE,-SLIGHT, FOCAL >EYES; RED DISCHARGE; EYE-LEFT; -MINERALIZATION, PELVIC,-MINIMAL, MULTI- VERIFIED AT NECROPSY: OBSERVED FOCAL >APPEARANCE; HUNCHED; VERIFIED AT MAMMARY, FEMALE (MF) : NECROPSY: NOT APPLICABLE -GALACTOCELE,-SLIGHT, MULTI-FOCAL >SKIN & PELAGE; ROUGH HAIR COAT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >BEHAVIOR; ATAXIC; VERIFIED AT -DILATATION, VENTRICULAR,-PRESENT NECROPSY: NOT APPLICABLE PITUITARY (PI) : PITUITARY (PI) : >BEHAVIOR; CIRCLING; RIGHT; VERIFIED AT -MASS(ES); 12 X 9 X 8 MM, RED, TAN AND -CYST,-PRESENT NECROPSY: NOT APPLICABLE FIRM -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DRY, RED MATERIAL -ANIMAL THIN -------------------------------------------- 4600 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 432 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92364 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 438 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 206.9 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 15:13 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4601 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 433 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92365 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 388.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:43 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0416 .0107 % .0191 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0382 .0098 % .0175 WEIGHT TAKEN BRAIN (BR) 2.1778 .5607 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.2772 .3288 % .5865 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.3060 .3363 % .5997 WEIGHT TAKEN LIVER (LI) 9.9235 2.5550 % 4.5567 WEIGHT TAKEN LUNG (LU) 1.7345 .4466 % .7964 WEIGHT TAKEN LEFT OVARY (OV0) .0670 .0173 % .0308 WEIGHT TAKEN RIGHT OVARY (OV1) .0635 .0163 % .0292 WEIGHT TAKEN SPLEEN (SP) .5717 .1472 % .2625 WEIGHT TAKEN LF THYROID/PARA (TT0) .0145 .0037 % .0067 WEIGHT TAKEN RT THYROID/PARA (TT1) .0137 .0035 % .0063 WEIGHT TAKEN UTERUS (UT) .9764 .2514 % .4483 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; DEATH COMMENT (DC) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 4602 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 434 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92365 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 388.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:43 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4603 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 435 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92366 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 215.3 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:59 PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 9.3217 4.3296 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : UNVERIFIED AT NECROPSY -NO MACROSCOPIC LESIONS -------------------------------------------- 4604 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 436 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92366 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 215.3 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:59 PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4605 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 437 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92367 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 468.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:47 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, FOCAL LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -FOCUS, ALTERED HEPATOCELLULAR, MULTIPLE DARK RED AREAS, UP TO 5 X 4 EOSINOPHILIC,-MODERATE, FOCAL MM; COLLECTED ON ROUTINE SECTIONS -HYPERPLASIA, BILE DUCT,-SLIGHT, MULTI- FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -TELANGIECTASIS,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT THYMUS (TH) : -CYST,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 5 X 3 MM, DARK RED, TAN -HEMORRHAGE,-MODERATE, MULTI-FOCAL AND SOFT -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4606 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 438 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92367 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 468.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:47 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4607 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 439 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92368 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/17/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 431.0 GRAMS DATE AND TIME OF NECROPSY: 03/17/00 8:26 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR,-SLIGHT, >MASS(ES); AXILLARY REGION-RIGHT; WEEK MULTI-FOCAL 91; 35 X 30MM; VERIFIED AT NECROPSY: THYROID (TY) : OBSERVED -FIBROSIS,-SLIGHT, FOCAL >MASS(ES); VENTRAL-ABDOMINAL; WEEK 100; -HYPERPLASIA, C-CELL,-MODERATE, FOCAL 40 X 30MM; VERIFIED AT NECROPSY: MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : OBSERVED -MASS(ES); LEFT PERINEAL REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL >APPEARANCE; HEAD TILT; LEFT; VERIFIED SINGLE MASS, 40 X 40 X 20 MM, TAN, -HEMORRHAGE,-MINIMAL, FOCAL AT NECROPSY: NOT APPLICABLE RED, GRAY AND SOFT; CUT SURFACE EXUDES -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT >EXCRETION; NO FECES; VERIFIED AT A TAN CREAMY SEMIFLUID; COLLECTED; NECROPSY: NOT APPLICABLE REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 40 X 30 X 15 MM, TAN, RED AND SOFT; CUT SURFACE EXUDES A TAN, CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 4608 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 440 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92368 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/17/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 431.0 GRAMS DATE AND TIME OF NECROPSY: 03/17/00 8:26 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); ATTACHED TO LEFT POSTERIOR -B-ADENOMA,-NEOPLASM PRESENT ASPECT: SINGLE MASS, 6 MM IN DIAMETER, DARK RED, RED AND SOFT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN AT BASE -------------------------------------------- 4609 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 441 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92368 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/17/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 431.0 GRAMS DATE AND TIME OF NECROPSY: 03/17/00 8:26 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4610 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 442 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92369 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 597.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:59 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK KIDNEY (KD) : 51; 115 X 110MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MODERATE, OBSERVED MULTI-FOCAL >MASS(ES); AXILLARY REGION-LEFT; WEEK LIVER (LI) : LIVER (LI) : 102; 25 X 20 MM; VERIFIED AT NECROPSY: -LARGE; ALL LOBES: APPEAR SWOLLEN >UNREMARKABLE OBSERVED THYROID (TY) : -B-ADENOMA, C-CELL,- 2 NEOPLASMS PRESENT SKIN (SK) : SKIN (SK) : >NOTE:>SKIN TAKEN FROM THE RIGHT SIDE -FIBROSIS,-MODERATE, MULTI-FOCAL DUE TO MASS ON THE LEFT SIDE MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL SINGLE MASS, 25 MM IN DIAMETER, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED AND FIRM; ON SECTION EXUDES A DARK -MINERALIZATION,-SLIGHT, FOCAL RED FLUID (CAP 21); REGIONAL LN -PIGMENT,-SLIGHT, MULTI-FOCAL (INGUINAL) NOT IDENTIFIED -B-FIBROADENOMA,-NEOPLASM PRESENT -MASS #2; LEFT ABDOMINAL REGION, -M-CARCINOMA,-NEOPLASM PRESENT EXTENDS TO LEFT INGUINAL REGION: SINGLE MASS, 110 X 100 X 40 MM (204.0 GRAMS), RED, DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED 4611 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 443 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92369 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 597.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:59 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : >NOTE:>MAMMARY TAKEN FROM THE RIGHT SIDE DUE TO MASS ON THE LEFT SIDE HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL SPLEEN (SP) : SPLEEN (SP) : -LARGE; 60 X 18 X 15 MM -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 10 X 8 X 8 MM (LEFT IS -DEGENERATION, CYSTIC, CORTICAL,- 4 X 3 X 2 MM) MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -VACUOLATION,-MODERATE, FOCAL -B-ADENOMA,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT POSTERIOR -HEMORRHAGE,-MODERATE, FOCAL ASPECT: SINGLE DARK RED AREA, 2 MM IN -B-ADENOMA,-NEOPLASM PRESENT DIAMETER HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 7 MM IN DIAMETER DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4612 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 444 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92369 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 597.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:59 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MEDIASTINAL (ML) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4613 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 445 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92369 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 597.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:59 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4614 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 446 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92370 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/02/00 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 572.0 GRAMS DATE AND TIME OF NECROPSY: 03/02/00 8:06 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK KIDNEY (KD) : KIDNEY (KD) : 80; 55 X 55MM; VERIFIED AT NECROPSY: -MOTTLED; BILATERAL: TAN AND BROWN -NEPHROPATHY, CHRONIC PROGRESSIVE,- OBSERVED MINIMAL, MULTI-FOCAL >MASS(ES); VENTRAL-THORACIC; WEEK 80; LIVER (LI) : LIVER (LI) : 45 X 35MM; VERIFIED AT NECROPSY: -RED FOCUS(I)/AREA(S); ALL LOBES: -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- OBSERVED MULTIPLE AREAS, UP TO 3 MM IN FOCAL >SKIN & PELAGE; SORE/SCAB; AXILLARY DIAMETER; COLLECTED WITH ROUTINE REGION-RIGHT; VERIFIED AT NECROPSY: SECTIONS OBSERVED MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL 100; 15 X 15MM; VERIFIED AT NECROPSY: SINGLE MASS, 12 X 21 X 35 MM, SOFT, -MINERALIZATION,-SLIGHT, MULTI-FOCAL OBSERVED TAN, DARK BROWN AND LOBULATED; ON -B-ADENOMA,-NEOPLASM PRESENT SECTION CONTAINS A DARK BROWN FLUID; -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 40 X 55 X 68 MM, FIRM, RED, TAN AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; OVERLYING SKIN HAS A DARK BROWN AND RED, DRY ABRADED AREA, 40 X 50 MM; COLLECTED WITH MASS; REGIONAL LN (SEE AXILLARY) 4615 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 447 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92370 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/02/00 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 572.0 GRAMS DATE AND TIME OF NECROPSY: 03/02/00 8:06 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #3; RIGHT CERVICAL REGION, EXTENDS TO MID VENTRAL THORACIC REGION: SINGLE MASS, 35 X 43 X 52 MM, FIRM, RED, TAN AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY) BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI- FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, FOCAL -HEMORRHAGE,-MODERATE, FOCAL PITUITARY (PI) : PITUITARY (PI) : -CYST(S); RIGHT ASPECT: SINGLE CYST, 4 -HEMORRHAGE,-SLIGHT, MULTI-FOCAL MM IN DIAMETER, FILLED WITH A DARK RED -B-ADENOMA,-NEOPLASM PRESENT FLUID DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; RIGHT: 4 X 8 X 10 MM (LEFT IS -HYPERPLASIA, LYMPHOCYTIC,-MINIMAL, 3 X 4 X 7 MM) (CAP 22) MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 4616 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 448 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92370 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/02/00 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 572.0 GRAMS DATE AND TIME OF NECROPSY: 03/02/00 8:06 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4617 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 449 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92371 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 619 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/15/99 14:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LUNG (LU) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI- FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -GALACTOCELE,-SLIGHT, MULTI-FOCAL AND FIRM; COLLECTED ON ROUTINE SECTION BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -THROMBUS,-MINIMAL, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 6 MM, RED, DARK RED -HEMORRHAGE,-MODERATELY SEVERE, MULTI- AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT DUODENUM (DU) : >AUTOLYTIC 4618 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 450 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92371 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 619 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/15/99 14:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ILEUM (IL) : >AUTOLYTIC UTERUS (UT) : UTERUS (UT) : -CYST(S); LEFT HORN, WITHIN WALL: -DILATATION,-MODERATE, FOCAL SINGLE CLEAR FLUID FILLED CYST, 4 X 3 X 3 MM DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- 4619 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 451 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92371 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 619 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/15/99 14:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4620 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 452 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92372 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 411.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:39 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0426 .0103 % .0198 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0380 .0092 % .0177 WEIGHT TAKEN BRAIN (BR) 2.1520 .5225 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.4414 .3499 % .6698 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.4720 .3574 % .6840 WEIGHT TAKEN LIVER (LI) 11.1932 2.7175 % 5.2013 WEIGHT TAKEN LUNG (LU) 1.7408 .4226 % .8089 WEIGHT TAKEN LEFT OVARY (OV0) .0816 .0198 % .0379 WEIGHT TAKEN RIGHT OVARY (OV1) .0713 .0173 % .0331 WEIGHT TAKEN SPLEEN (SP) .6446 .1565 % .2995 WEIGHT TAKEN LF THYROID/PARA (TT0) .0146 .0035 % .0068 WEIGHT TAKEN RT THYROID/PARA (TT1) .0172 .0042 % .0080 WEIGHT TAKEN UTERUS (UT) .9058 .2199 % .4209 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : VERIFIED AT NECROPSY: OBSERVED -CYST(S); ALL REGIONS DIFFUSE: >UNREMARKABLE MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 4 X 3 X 2 MM; COLLECTED ON ROUTINE SECTION LN, MESENTERIC (MS) : -EDEMA,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 7 X 6 X 4 MM >UNREMARKABLE STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: TWO -EDEMA,-MODERATELY SEVERE, FOCAL BROWN AREAS, EACH IS 3 X 2 MM 4621 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 453 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92372 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 411.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:39 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4622 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 454 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92373 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 187.3 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:29 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.2075 3.8481 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : UNVERIFIED AT NECROPSY -NO MACROSCOPIC LESIONS -------------------------------------------- 4623 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 455 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92373 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 187.3 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:29 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4624 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 456 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92374 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 572.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:41 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL -TELANGIECTASIS,-MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT EYE (EY) : -INFLAMMATION, ACUTE, CORNEAL,-MINIMAL, MULTI-FOCAL -VASCULARIZATION, CORNEAL,-MINIMAL, MULTI-FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 4625 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 457 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92374 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 572.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:41 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 6 MM, GRAY, RED AND -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-MINIMAL, FOCAL CERVIX (CV) : -B-LEIOMYOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL (CAP 22) ADIPOSE TISSUE (AT) : -M-LIPOSARCOMA,-NEOPLASM PRESENT LN, POPLITEAL (PP) : LN, POPLITEAL (PP) : -LARGE; LEFT: 9 MM IN DIAMETER (RIGHT >TISSUE MISSING NOT IDENTIFIED) >NOTE:>POPLITEAL LYMPH NODE DESCRIBED -MOTTLED; LEFT: RED AND TAN (CAP 23) GROSSLY WAS A SUBCUTANEOUS LIPOSARCOMA GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN AT BASE -------------------------------------------- 4626 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 458 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92374 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 572.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:41 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4627 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 459 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92375 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 427.1 GRAMS DATE AND TIME OF NECROPSY: 12/01/99 12:58 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; YELLOW HAIR COAT; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, MULTI- >EXCRETION; NO FECES; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >EYES; RED DISCHARGE; EYE-LEFT; -MOTTLED; ALL LOBES: BROWN AND TAN -HYPERPLASIA, BILE DUCT,-MODERATE, VERIFIED AT NECROPSY: OBSERVED MULTI-FOCAL >APPEARANCE; HUNCHED; VERIFIED AT -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE FOCAL >DISCHARGE; NASAL; RED IN COLOR; -VACUOLATION, HEPATOCELLULAR,-SLIGHT, VERIFIED AT NECROPSY: OBSERVED MULTI-FOCAL PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 8 MM, RED, DARK -HEMORRHAGE,-MODERATE, FOCAL RED, TAN, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL 4628 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 460 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92375 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 427.1 GRAMS DATE AND TIME OF NECROPSY: 12/01/99 12:58 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ OVARY (OV) : -CYST,-PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; YELLOW -DISCHARGE-OCULAR; BILATERAL: RED -DISCHARGE-NASAL; BILATERAL: RED -------------------------------------------- 4629 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 461 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92375 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 427.1 GRAMS DATE AND TIME OF NECROPSY: 12/01/99 12:58 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4630 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 462 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92376 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 321.1 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:31 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0430 .0134 % .0202 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0500 .0156 % .0235 WEIGHT TAKEN BRAIN (BR) 2.1321 .6640 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.2378 .3855 % .5806 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.2613 .3928 % .5916 WEIGHT TAKEN LIVER (LI) 9.9801 3.1081 % 4.6809 WEIGHT TAKEN LUNG (LU) 1.5665 .4879 % .7347 WEIGHT TAKEN LEFT OVARY (OV0) .0449 .0140 % .0211 WEIGHT TAKEN RIGHT OVARY (OV1) .0412 .0128 % .0193 WEIGHT TAKEN SPLEEN (SP) .5194 .1618 % .2436 WEIGHT TAKEN LF THYROID/PARA (TT0) .0119 .0037 % .0056 WEIGHT TAKEN RT THYROID/PARA (TT1) .0097 .0030 % .0045 WEIGHT TAKEN UTERUS (UT) 1.2052 .3753 % .5653 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL; WEEK 46; MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : 30 X 30MM; VERIFIED AT NECROPSY: -MASS(ES); RIGHT AXILLARY REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT OBSERVED SINGLE MASS, 45 X 30 X 25 MM, RED, TAN, GRAY AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) THYMUS (TH) : -CYST,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, FOCAL 4631 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 463 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92376 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 321.1 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:31 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -DIFFUSELY RED; LEFT; RIGHT IS NORMAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL (CAP 18) -INFILTRATE, MACROPHAGE, VACUOLATED,- MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 4632 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 464 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92376 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 321.1 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:31 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4633 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 465 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92377 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/03/99 STUDY DAY OF DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/03/99 11:06 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : NECROPSY: OBSERVED -MASS(ES); VENTRAL CERVICAL REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK SINGLE MASS, 30 X 17 X 15 MM, RED, -B-ADENOMA,-NEOPLASM PRESENT 31; 20 X 20MM; VERIFIED AT NECROPSY: DARK RED, TAN, FIRM AND LOBULATED; OBSERVED SAME ON CUT SURFACE (CAP 18); REGIONAL LN (MANDIBULAR) NORMAL (CAP 19) BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 7 X 5 MM, -B-ADENOMA,-NEOPLASM PRESENT RED, DARK RED, TAN AND SOFT ILEUM (IL) : >AUTOLYTIC OVARY (OV) : -INFLAMMATION, CHRONIC,-MODERATE, MULTI- FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL UPPER INCISORS -DISCHARGE-OCULAR; RED, DRY MATERIAL -DISCHARGE-NASAL; RED, DRY MATERIAL -------------------------------------------- 4634 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 466 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92377 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/03/99 STUDY DAY OF DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/03/99 11:06 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4635 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 467 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92378 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/26/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 292.2 GRAMS DATE AND TIME OF NECROPSY: 08/26/99 7:45 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; VENTRAL- KIDNEY (KD) : ABDOMINAL; VERIFIED AT NECROPSY: NOT -MINERALIZATION, PELVIC,-MODERATE, OBSERVED FOCAL >APPEARANCE; THIN; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT OBSERVED MODERATE, MULTI-FOCAL >EXCRETION; FEW FECES; VERIFIED AT THYROID (TY) : NECROPSY: NOT APPLICABLE -CYST, ULTIMOBRANCHIAL,-PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : NECROPSY: NOT APPLICABLE -THICKENED; ALL REGIONS, DIFFUSE: TAN; -GALACTOCELE,-SLIGHT, MULTI-FOCAL >SKIN & PELAGE; YELLOW HAIR COAT; COLLECTED ON ROUTINE SECTION VERIFIED AT NECROPSY: NOT OBSERVED BRAIN (BR) : BRAIN (BR) : >BEHAVIOR; ATAXIC; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE HEART (HT) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -DEGENERATION/NECROSIS,-MINIMAL, MULTI- NECROPSY: OBSERVED FOCAL THYMUS (TH) : -CYST,-PRESENT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL -PIGMENT,-MINIMAL, FOCAL -THROMBUS,-SLIGHT, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 4636 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 468 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92378 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/26/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 292.2 GRAMS DATE AND TIME OF NECROPSY: 08/26/99 7:45 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -MASS(ES); 7 X 7 X 5 MM, RED, DARK RED AND TAN STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA, NEAR -EDEMA,-MODERATE, DIFFUSE JUNCTION OF GLANDULAR MUCOSA: SINGLE -EROSION,-SEVERE, FOCAL TAN ERODED AREA, 5 MM IN DIAMETER -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 3 MM IN DIAMETER DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK BROWN AND DRY MATERIAL -DISCHARGE-NASAL; DARK BROWN AND DRY MATERIAL -------------------------------------------- 4637 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 469 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92378 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/26/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 292.2 GRAMS DATE AND TIME OF NECROPSY: 08/26/99 7:45 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4638 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 470 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92379 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/16/99 10:41 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK KIDNEY (KD) : 49; 20 X 15MM; VERIFIED AT NECROPSY: -CAST, PROTEINACEOUS,-MINIMAL, MULTI- OBSERVED FOCAL >MASS(ES); VENTRAL-THORACIC; WEEK 70; SKIN (SK) : 10 X 10MM; VERIFIED AT NECROPSY: >NOTE:>COLLECTED FROM RIGHT SIDE DUE TO OBSERVED MAMMARY GLAND MASS >APPEARANCE; THIN; VERIFIED AT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : NECROPSY: NOT OBSERVED -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL >EXCRETION; FEW FECES; VERIFIED AT SINGLE MASS, 29 X 19 X 11 MM, RED, -B-ADENOMA,- 2 NEOPLASMS PRESENT NECROPSY: NOT APPLICABLE DARK RED, TAN, LOBULATED AND VASCULAR; SOME SECTIONS EXUDE A DARK BROWN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -CYST(S); RIGHT AXILLARY REGION: SINGLE TAN FLUID FILLED CYST, 12 MM IN DIAMETER; COLLECTED; POSSIBLY CONSISTENT WITH MASS (RIGHT AXILLARY) AS NOTED IN CLINICAL OBSERVATIONS -MASS #2; RIGHT VENTRAL CERVICAL REGION: SINGLE MASS, 13 X 9 X 4 MM, TAN, RED AND LOBULATED; ON SECTION EXUDES A TAN CASEOUS SEMIFLUID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 18) 4639 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 471 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92379 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/16/99 10:41 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : >NOTE:>COLLECTED FROM RIGHT SIDE DUE TO MAMMARY GLAND MASS EYE (EY) : >NOTE:>LEFT EYE RUPTURED AT NECROPSY BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 13 X 10 X 6 -HEMORRHAGE,-SEVERE, MULTI-FOCAL MM, RED, DARK RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT -------------------------------------------- 4640 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 472 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92379 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/16/99 10:41 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4641 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 473 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92380 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 338.1 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 10:55 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, MULTI- >MASS(ES); AXILLARY REGION-RIGHT; WEEK FOCAL 60; 45 X 45MM; VERIFIED AT NECROPSY: -NEPHROPATHY, CHRONIC PROGRESSIVE,- OBSERVED SLIGHT, MULTI-FOCAL >APPEARANCE; THIN; VERIFIED AT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : NECROPSY: OBSERVED -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL >EXCRETION; FEW FECES; VERIFIED AT SINGLE MASS, 45 X 40 X 20 MM, TAN, -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT NECROPSY: NOT APPLICABLE FIRM, AND VASCULAR; SAME ON CUT >BEHAVIOR; ATAXIC; VERIFIED AT SURFACE; COLLECTED; REGIONAL LN NECROPSY: NOT APPLICABLE (AXILLARY) NORMAL (CAP 18) -CYST(S); ALL REGIONS DIFFUSE: MULTIPLE YELLOW CREAMY SEMIFLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION LEFT AXILLARY REGION: SINGLE BROWN SEMIFLUID FILLED CYST, 15 MM IN DIAMETER; COLLECTED (CAP 19) -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 20 X 20 X 8 MM, TAN, LOBULATED, AND SOFT; ON SECTION, EXUDES A LIGHT GREEN SEMISOLID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 18) BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT 4642 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 474 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92380 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 338.1 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 10:55 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -THROMBUS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 12 X 8 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- SOFT, AND CYSTIC FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI- FOCAL VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE YELLOW CASEOUS MATERIAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL: UP TO 10 X 7 X 3 MM -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: LOWER INCISORS AND LEFT UPPER INCISOR -ANIMAL THIN -TEETH MISSING/BROKEN/WORN; RIGHT UPPER INCISOR: BROKEN OFF AT BASE 4643 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 475 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92380 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 338.1 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 10:55 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), LN, AXILLARY (AX), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4644 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 476 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92381 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/29/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 299.5 GRAMS DATE AND TIME OF NECROPSY: 07/29/99 8:10 PROSECTOR: JILL PAUS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NO FECES; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >SKIN & PELAGE; YELLOW HAIR COAT; FOCAL VERIFIED AT NECROPSY: NOT OBSERVED -MINERALIZATION, PELVIC,-MODERATE, >APPEARANCE; THIN; VERIFIED AT MULTI-FOCAL NECROPSY: NOT OBSERVED MAMMARY, FEMALE (MF) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -GALACTOCELE,-MODERATE, MULTI-FOCAL NECROPSY: OBSERVED BRAIN (BR) : BRAIN (BR) : >DISCHARGE; NASAL; RED IN COLOR; -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: OBSERVED THYMUS (TH) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -CYST,-PRESENT NECROPSY: NOT APPLICABLE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 5 X 3 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 4645 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 477 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92381 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/29/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 299.5 GRAMS DATE AND TIME OF NECROPSY: 07/29/99 8:10 PROSECTOR: JILL PAUS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: RED DRY MATERIAL -DISCHARGE-NASAL; RED DRY MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4646 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 478 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92382 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/03/99 STUDY DAY OF DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: 231.0 GRAMS DATE AND TIME OF NECROPSY: 05/03/99 11:13 PROSECTOR: MICHELE MARGGI RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, FOCAL >EYES; SQUINTED; PERI-ORBITAL-RIGHT; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -RED FOCUS(I)/AREA(S); LEFT LATERAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, >EYES; RED DISCHARGE; EYES; VERIFIED AT LOBE: SINGLE DARK RED AREA, 5 X 2 MM MULTI-FOCAL NECROPSY: OBSERVED (COLLECTED ON ROUTINE SECTION) >SKIN & PELAGE; BROWN HAIR COAT; MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : GENERALIZED; VERIFIED AT NECROPSY: -THICKENED; ALL REGIONS, DIFFUSE: TAN -GALACTOCELE,-MINIMAL, MULTI-FOCAL OBSERVED AND FIRM (COLLECTED WITH ROUTINE >EXCRETION; FEW FECES; VERIFIED AT SECTION) NECROPSY: NOT APPLICABLE BRAIN (BR) : BRAIN (BR) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE -DILATATION, VENTRICULAR,-PRESENT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE, MULTI- FOCAL THYMUS (TH) : -CYST,-PRESENT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 9 MM IN DIAMETER (FOR -THROMBUS,-SEVERE, FOCAL COMPARISON RIGHT IS 5 MM IN DIAMETER) PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 8 X 6 MM, -B-ADENOMA,-NEOPLASM PRESENT RED, DARK RED, TAN AND SOFT 4647 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 479 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92382 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/03/99 STUDY DAY OF DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: 231.0 GRAMS DATE AND TIME OF NECROPSY: 05/03/99 11:13 PROSECTOR: MICHELE MARGGI RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EROSION,-MODERATE, FOCAL MULTIPLE DARK BROWN AREAS, UP TO 4 X 2 -INFLAMMATION, ACUTE,-MINIMAL, FOCAL MM STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA, ALONG -EDEMA,-MODERATE, DIFFUSE GREATER CURVATURE: MULTIPLE TAN ERODED -EROSION,-MODERATELY SEVERE, FOCAL AREAS, UP TO 5 X 2 MM URINARY BLADDER (UB) : URINARY BLADDER (UB) : -CALCULUS(I); WITHIN LUMEN: MULTIPLE -HYPERPLASIA, TRANSITIONAL CELL,- HARD TAN CALCULI, UP TO 20 X 10 X 10 MODERATE, DIFFUSE MM -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; BROWN AND MOIST -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY CRUSTED MATERIAL -ANIMAL THIN -------------------------------------------- 4648 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 480 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92382 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/03/99 STUDY DAY OF DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: 231.0 GRAMS DATE AND TIME OF NECROPSY: 05/03/99 11:13 PROSECTOR: MICHELE MARGGI RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4649 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 481 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92383 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 554.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:33 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK KIDNEY (KD) : 96; 40 X 40MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MODERATE, OBSERVED MULTI-FOCAL >SKIN & PELAGE; RED HAIR COAT; HEAD- LIVER (LI) : LIVER (LI) : CRANIAL; VERIFIED AT NECROPSY: NOT -MOTTLED; ALL LOBES: RED AND TAN -CYST, BILIARY,-MODERATE, FOCAL OBSERVED -DEGENERATION, CYSTIC,-MODERATE, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 35 X 30 X 7 MM, RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) BRAIN (BR) : -INFLAMMATION, CHRONIC,-SLIGHT, FOCAL 4650 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 482 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92383 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 554.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:33 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -CYST,-PRESENT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -MASS(ES); INVOLVING ENTIRE LEFT -ANGIECTASIS,-SEVERE, FOCAL ADRENAL: SINGLE MASS, 17 MM IN -HEMORRHAGE,-MODERATE, MULTI-FOCAL DIAMETER, RED, DARK RED, TAN AND FIRM; -THROMBUS,-SLIGHT, MULTI-FOCAL SAME ON CUT SURFACE; REGIONAL LN (RENAL) NOT IDENTIFIED PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : JEJUNUM (JE) : -DIVERTICULUM(A); MID JEJUNUM: SINGLE >UNREMARKABLE DIVERTICULUM, 8 X 5 X 3 MM; COLLECTED ON ROUTINE SECTION UTERUS (UT) : UTERUS (UT) : -MASS(ES); WITHIN WALL OF RIGHT HORN: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO SINGLE MASS, 20 X 15 X 10 MM, RED, NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 24) OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 5 MM IN DIAMETER -B-GRANULOSA/THECA CELL TUMOR,-NEOPLASM -MASS(ES); RIGHT: SINGLE MASS, 30 X 25 PRESENT X 20 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 24) 4651 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 483 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92383 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 554.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:33 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT CAVITY, ABDOM (PC) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4652 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 484 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92383 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 554.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:33 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 4653 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 485 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92384 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/10/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 320.7 GRAMS DATE AND TIME OF NECROPSY: 12/10/99 9:48 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NO FECES; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -CAST, PROTEINACEOUS,-MINIMAL, FOCAL >EYES; RED DISCHARGE; EYES; VERIFIED AT -MINERALIZATION, PELVIC,-SLIGHT, MULTI- NECROPSY: OBSERVED FOCAL >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -TELANGIECTASIS,-MINIMAL, MULTI-FOCAL >SKIN & PELAGE; BROWN HAIR COAT; MULTIPLE AREAS, UP TO 3 MM IN -VACUOLATION, HEPATOCELLULAR,-MINIMAL, VENTRAL-ABDOMINAL; VERIFIED AT DIAMETER; COLLECTED ON ROUTINE SECTION MULTI-FOCAL NECROPSY: OBSERVED -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM >BEHAVIOR; HYPOACTIVE; VERIFIED AT PRESENT NECROPSY: NOT APPLICABLE PANCREAS (PA) : PANCREAS (PA) : >SKIN & PELAGE; PALE; ENTIRE BODY; -MASS(ES); ALONG SPLEEN: SINGLE MASS, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE 10 X 10 X 6 MM, TAN, RED AND FIRM; -B-ADENOMA, ISLET CELL,-NEOPLASM SAME ON CUT SURFACE; COLLECTED INTACT; PRESENT REGIONAL LN (PANCREATIC) NOT IDENTIFIED THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -GALACTOCELE,-SLIGHT, MULTI-FOCAL AND SOFT BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT 4654 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 486 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92384 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/10/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 320.7 GRAMS DATE AND TIME OF NECROPSY: 12/10/99 9:48 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -CYST,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- GRAY AND SOFT FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE -EDEMA,-MODERATELY SEVERE, MULTI-FOCAL -EROSION/ULCERATION; MUCOSA: MULTIPLE -INFLAMMATION, ACUTE,-MODERATE, MULTI- BROWN AND TAN ULCERATED AREAS, UP TO 6 FOCAL X 5 MM -ULCERATION,-SEVERE, MULTI-FOCAL UTERUS (UT) : -DILATATION,-MINIMAL, FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; PERINEUM: MOIST DARK BROWN MATERIAL -DISCHARGE-OCULAR; BILATERAL: DRY DARK BROWN MATERIAL -ANIMAL THIN -GI TRACT-DARK FLUID CONTENT; ILEUM AND CECUM: CONTAINS A DARK BROWN SEMIFLUID MATERIAL 4655 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 487 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92384 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/10/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 320.7 GRAMS DATE AND TIME OF NECROPSY: 12/10/99 9:48 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4656 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 488 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92385 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/31/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 334.6 GRAMS DATE AND TIME OF NECROPSY: 01/31/00 13:35 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NO FECES; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -INFARCT,-SLIGHT, FOCAL >APPEARANCE; HEAD TILT; LEFT; VERIFIED -MINERALIZATION, PELVIC,-SLIGHT, MULTI- AT NECROPSY: NOT APPLICABLE FOCAL >APPEARANCE; THIN; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: OBSERVED MODERATE, MULTI-FOCAL LIVER (LI) : LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN -HEMORRHAGE,-SLIGHT, FOCAL -VACUOLATION, HEPATOCELLULAR,-SEVERE, DIFFUSE THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: -GALACTOCELE,-MODERATE, MULTI-FOCAL MULTIPLE TAN, CREAMY SEMIFLUID FILLED -B-FIBROADENOMA,-NEOPLASM PRESENT CYSTS, UP TO 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 14 X 12 X 10 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, BROWN, AND SOFT 4657 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 489 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92385 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/31/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 334.6 GRAMS DATE AND TIME OF NECROPSY: 01/31/00 13:35 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : UTERUS (UT) : -CYST(S); WITHIN WALL OF RIGHT HORN AT >UNREMARKABLE BIFURCATION: SINGLE DARK RED FLUID FILLED CYST, 4 MM IN DIAMETER VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE LIGHT GREEN, MUCOID SEMIFLUID DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 4658 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 490 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92385 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/31/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 334.6 GRAMS DATE AND TIME OF NECROPSY: 01/31/00 13:35 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4659 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 491 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92386 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 308.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:14 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL; WEEK 80; LUNG (LU) : 40 X 40MM; VERIFIED AT NECROPSY: -INFLAMMATION, GRANULOMATOUS,-SLIGHT, OBSERVED MULTI-FOCAL >WART LIKE LESION(S); EAR-LEFT; WEEK KIDNEY (KD) : 92; VERIFIED AT NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK SLIGHT, MULTI-FOCAL 95; 10 X 10MM; VERIFIED AT NECROPSY: THYROID (TY) : OBSERVED -CYST, ULTIMOBRANCHIAL,-PRESENT >SKIN & PELAGE; SORE/SCAB; VENTRAL- -B-ADENOMA, C-CELL,-NEOPLASM PRESENT CERVICAL-LEFT; VERIFIED AT NECROPSY: SKIN (SK) : SKIN (SK) : OBSERVED -CRUSTED AREA(S); WITHIN LEFT PINNA: -B-ADENOMA, SEBACEOUS GLAND,-NEOPLASM >MASS(ES); INGUINAL REGION-LEFT; WEEK SINGLE TAN CRUSTED AREA, 5 X 3 MM; PRESENT 101; 30 X 30MM; VERIFIED AT NECROPSY: COLLECTED INTACT OBSERVED MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : >APPEARANCE; THIN; VERIFIED AT -MASS(ES); LEFT AXILLARY REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL NECROPSY: OBSERVED SINGLE MASS, 23 X 15 X 15 MM, BROWN, -HEMORRHAGE,-MODERATE, MULTI-FOCAL TAN, LOBULATED AND FIRM; SAME ON CUT -INFLAMMATION, ACUTE,-MODERATELY SURFACE; OVERLYING SKIN HAS A TAN, SEVERE, MULTI-FOCAL MOIST, ULCERATED AREA, 1 MM IN -MINERALIZATION,-SLIGHT, MULTI-FOCAL DIAMETER; COLLECTED INTACT WITH MASS -B-ADENOMA,-NEOPLASM PRESENT (CAP 22); REGIONAL LN (AXILLARY) -M-CARCINOMA,-NEOPLASM PRESENT NORMAL (CAP 23); POSSIBLY CONSISTENT WITH MASS (LEFT CERVICAL ABDOMINAL REGION) NOTED IN CLINICAL OBSERVATIONS 4660 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 492 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92386 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 308.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:14 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 53 X 41 X 24 MM, TAN, RED, LOBULATED, AND FIRM; SOME SECTIONS EXUDE A TAN AND GREEN SEMIFLUID; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH MASSES (VENTRAL ABDOMINAL AND LEFT INGUINAL REGIONS) AS NOTED IN CLINICAL OBSERVATIONS ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -VACUOLATION,-MODERATE, FOCAL -B-ADENOMA,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 5 X 4 MM -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4661 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 493 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92386 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 308.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:14 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4662 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 494 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92387 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/31/99 STUDY DAY OF DEATH: 513 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 341.8 GRAMS DATE AND TIME OF NECROPSY: 08/31/99 14:41 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK KIDNEY (KD) : 59; 45 X 30MM; VERIFIED AT NECROPSY: -CAST, PROTEINACEOUS,-MINIMAL, MULTI- OBSERVED FOCAL >APPEARANCE; THIN; VERIFIED AT -MINERALIZATION, PELVIC,-SLIGHT, MULTI- NECROPSY: OBSERVED FOCAL >SKIN & PELAGE; RED HAIR COAT; VENTRAL- LIVER (LI) : ABDOMINAL; VERIFIED AT NECROPSY: -VACUOLATION, HEPATOCELLULAR,- OBSERVED MODERATELY SEVERE, MULTI-FOCAL >EYES; SQUINTED; PERI-ORBITAL; VERIFIED THYROID (TY) : AT NECROPSY: NOT APPLICABLE -CYST, ULTIMOBRANCHIAL,-PRESENT >SKIN & PELAGE; RED HAIR COAT; PERINEAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT AREA; VERIFIED AT NECROPSY: OBSERVED MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 43 X 25 X 20 MM, TAN AND -B-FIBROADENOMA,-NEOPLASM PRESENT FIRM; ON SECTION, EXUDES A TAN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 18) BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL 4663 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 495 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92387 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/31/99 STUDY DAY OF DEATH: 513 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 341.8 GRAMS DATE AND TIME OF NECROPSY: 08/31/99 14:41 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 5 MM, DARK RED AND -HEMORRHAGE,-MINIMAL, MULTI-FOCAL FIRM -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; ALL REGIONS, DIFFUSE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, -EROSION/ULCERATION; MUCOSA: SINGLE MULTI-FOCAL DARK RED ERODED AREA, 10 X 5 MM -ULCERATION,-SEVERE, FOCAL UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -DILATATION,-MODERATELY SEVERE, DIFFUSE MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 15 X 5 X 5 MM DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, MEDIASTINAL (ML) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; DRY RED MATERIAL -ANIMAL THIN 4664 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 496 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92387 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/31/99 STUDY DAY OF DEATH: 513 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 341.8 GRAMS DATE AND TIME OF NECROPSY: 08/31/99 14:41 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -STAINS - SKIN/HAIRCOAT; ENTIRE ABDOMINAL REGION: DRY RED MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4665 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 497 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92388 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 436.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:45 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0614 .0141 % .0284 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0410 .0094 % .0190 WEIGHT TAKEN BRAIN (BR) 2.1627 .4950 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.3578 .3108 % .6278 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.3404 .3068 % .6198 WEIGHT TAKEN LIVER (LI) 11.3882 2.6066 % 5.2657 WEIGHT TAKEN LUNG (LU) 1.6427 .3760 % .7596 WEIGHT TAKEN LEFT OVARY (OV0) .0493 .0113 % .0228 WEIGHT TAKEN RIGHT OVARY (OV1) .0437 .0100 % .0202 WEIGHT TAKEN SPLEEN (SP) .5916 .1354 % .2735 WEIGHT TAKEN LF THYROID/PARA (TT0) .0145 .0033 % .0067 WEIGHT TAKEN RT THYROID/PARA (TT1) .0155 .0035 % .0072 WEIGHT TAKEN UTERUS (UT) 1.0659 .2440 % .4929 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LUNG (LU) : VERIFIED AT NECROPSY: OBSERVED -HEMORRHAGE,-MINIMAL, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -INFLAMMATION, CHRONIC,-MODERATE, MULTI- FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 4666 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 498 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92388 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 436.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:45 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY -------------------------------------------- 4667 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 499 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92388 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 436.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:45 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4668 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 500 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92389 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 356.5 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:45 PROSECTOR: MONTI THOMAS MAGUIRE RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .1123 .0315 % .0556 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0474 .0133 % .0235 WEIGHT TAKEN BRAIN (BR) 2.0204 .5667 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.3505 .3788 % .6684 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.4494 .4066 % .7174 WEIGHT TAKEN LIVER (LI) 9.5787 2.6869 % 4.7410 WEIGHT TAKEN LUNG (LU) 2.2838 .6406 % 1.1304 WEIGHT TAKEN LEFT OVARY (OV0) .0660 .0185 % .0327 WEIGHT TAKEN RIGHT OVARY (OV1) .0684 .0192 % .0339 WEIGHT TAKEN SPLEEN (SP) .4422 .1240 % .2189 WEIGHT TAKEN LF THYROID/PARA (TT0) .0103 .0029 % .0051 WEIGHT TAKEN RT THYROID/PARA (TT1) .0114 .0032 % .0056 WEIGHT TAKEN UTERUS (UT) 1.2955 .3634 % .6412 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LUNG (LU) : LUNG (LU) : VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, MACROPHAGE, ALVEOLAR,- MULTIPLE WHITE AREAS, UP TO 3 MM IN MODERATE, MULTI-FOCAL DIAMETER PANCREAS (PA) : >TISSUE MISSING ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 10 X 8 X 7 MM (FOR -ANGIECTASIS,-SLIGHT, FOCAL COMPARISON RIGHT IS 5 X 3 X 3 MM) -HEMORRHAGE,-SLIGHT, FOCAL RECTUM (RE) : -PARASITISM,-PRESENT 4669 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 501 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92389 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 356.5 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:45 PROSECTOR: MONTI THOMAS MAGUIRE RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), LIVER (LI), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4670 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 502 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92390 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 341.2 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:39 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0368 .0108 % .0183 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0211 .0062 % .0105 WEIGHT TAKEN BRAIN (BR) 2.0064 .5880 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.1624 .3407 % .5793 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.0052 .2946 % .5010 WEIGHT TAKEN LIVER (LI) 9.1787 2.6901 % 4.5747 WEIGHT TAKEN LUNG (LU) 1.5087 .4422 % .7519 WEIGHT TAKEN LEFT OVARY (OV0) .0798 .0234 % .0398 WEIGHT TAKEN RIGHT OVARY (OV1) .0865 .0254 % .0431 WEIGHT TAKEN SPLEEN (SP) .6107 .1790 % .3044 WEIGHT TAKEN LF THYROID/PARA (TT0) .0175 .0051 % .0087 WEIGHT TAKEN RT THYROID/PARA (TT1) .0151 .0044 % .0075 WEIGHT TAKEN UTERUS (UT) .6848 .2007 % .3413 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, FOCAL LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -HEMORRHAGE,-SLIGHT, FOCAL MULTIPLE RED AREAS, UP TO 2 X 1 MM; -NECROSIS, HEPATOCELLULAR,-SLIGHT, COLLECTED ON ROUTINE SECTIONS FOCAL ADRENAL, CORTEX (AC) : -ONE EXAMINED,-PRESENT ADRENAL, MEDULLA (MA) : -ONE EXAMINED,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 4 X 3 MM; APPEARS SWOLLEN -HEMORRHAGE,-SLIGHT, FOCAL 4671 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 503 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92390 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 341.2 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:39 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); DIFFUSE: -B-ADENOMA,-NEOPLASM PRESENT MULTIPLE DARK RED AREAS, UP TO 1 MM IN DIAMETER DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 4672 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 504 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92390 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 341.2 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:39 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4673 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 505 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92391 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 10:36 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK LUNG (LU) : 61; 20 X 30MM; VERIFIED AT NECROPSY: -HYPERPLASIA, TYPE II PNEUMOCYTE,- NOT OBSERVED MODERATE, FOCAL >EYES; RED DISCHARGE; EYE-LEFT; -INFILTRATE, MACROPHAGE, ALVEOLAR,- VERIFIED AT NECROPSY: OBSERVED SLIGHT, FOCAL >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; KIDNEY (KD) : KIDNEY (KD) : WEEK 84; 20 X 15MM; VERIFIED AT -GRANULAR MATERIAL; BILATERAL, WITHIN -CAST, PROTEINACEOUS,-MINIMAL, MULTI- NECROPSY: OBSERVED PELVIS: MULTIPLE TAN AND FIRM FOCAL >MASS(ES); INGUINAL REGION-RIGHT; WEEK GRANULES, UP TO 1 MM IN DIAMETER -MINERALIZATION, PELVIC,-SLIGHT, MULTI- 84; 10 X 10MM; VERIFIED AT NECROPSY: FOCAL OBSERVED LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT VENTRAL CERVICAL -GALACTOCELE,-MODERATELY SEVERE, MULTI- REGION: SINGLE MASS, 35 X 25 X 15 MM, FOCAL TAN, GRAY, AND FIRM; CUT SURFACE -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT CONTAINS A TAN CASEOUS SEMISOLID; -B-FIBROMA,-NEOPLASM PRESENT COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 18) 4674 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 506 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92391 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 10:36 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 38 X 25 X 15 MM, DARK RED, TAN, AND SOFT; CUT SURFACE CONTAINS A TAN CASEOUS SEMISOLID; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 19) -MASS #3; RIGHT ABDOMINAL REGION: SINGLE MASS, 24 MM IN DIAMETER, DARK RED AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 19) BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT HEART (HT) : -FIBROSIS,-SLIGHT, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL -THROMBUS,-SLIGHT, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 10 X 7 MM, DARK RED, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL TAN, AND SOFT -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 4675 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 507 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92391 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 10:36 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ILEUM (IL) : >AUTOLYTIC DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND MOIST -------------------------------------------- 4676 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 508 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92391 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 10:36 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, INGUINAL (IN), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4677 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 509 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92392 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 442 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 286.8 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 12:39 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >DISCHARGE; NASAL; RED IN COLOR; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, MULTI- >APPEARANCE; HEAD TILT; LEFT; VERIFIED FOCAL AT NECROPSY: NOT APPLICABLE LIVER (LI) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -VACUOLATION, HEPATOCELLULAR,-MODERATE, NECROPSY: OBSERVED MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -HYPERPLASIA, GLANDULAR,-MODERATE, FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 4 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT STOMACH, NONGL (SU) : -EDEMA,-MODERATE, DIFFUSE OVARY (OV) : -CYST,-PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4678 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 510 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92392 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 442 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 286.8 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 12:39 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -DISCHARGE-NASAL; DRY RED MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4679 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 511 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92393 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/18/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 517.2 GRAMS DATE AND TIME OF NECROPSY: 01/18/00 10:44 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK KIDNEY (KD) : 73; 20 X 25MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MINIMAL, FOCAL OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >MASS(ES); AXILLARY REGION-RIGHT; WEEK MODERATE, MULTI-FOCAL 60; 110 X 90MM; VERIFIED AT NECROPSY: LIVER (LI) : OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY,- >MASS(ES); INGUINAL REGION-LEFT; WEEK MINIMAL, MULTI-FOCAL 92; 15 X 15MM; VERIFIED AT NECROPSY: -VACUOLATION, HEPATOCELLULAR,-MINIMAL, OBSERVED MULTI-FOCAL >APPEARANCE; THIN; VERIFIED AT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : NECROPSY: OBSERVED -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL >APPEARANCE; MALOCCLUSION; VERIFIED AT SINGLE MASS, 105 X 75 X 43 MM, TAN, -MINERALIZATION,-MINIMAL, MULTI-FOCAL NECROPSY: NOT OBSERVED LOBULATED AND FIRM; SAME ON CUT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT SURFACE; COLLECTED INTACT; REGIONAL LN -M-CARCINOMA,-NEOPLASM PRESENT (SEE AXILLARY) -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 30 X 30 X 15 MM, GREEN, TAN, LOBULATED AND SOFT; ON SECTION, EXUDES A GREEN SEMIFLUID; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED 4680 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 512 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92393 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/18/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 517.2 GRAMS DATE AND TIME OF NECROPSY: 01/18/00 10:44 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #3; LEFT INGUINAL REGION, CRANIAL TO MASS 2: SINGLE MASS, 30 X 23 X 15 MM, TAN, RED AND FIRM; ON SECTION, EXUDES A TAN CREAMY SEMIFLUID (CAP 19); REGIONAL LN (INGUINAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL LEFT) NOTED IN CLINICAL OBSERVATIONS MARROW, FEMUR (FM) : -HYPERCELLULARITY, MARROW,-MODERATE, DIFFUSE MARROW, STERNUM (SE) : -HYPERCELLULARITY, MARROW,-MODERATE, DIFFUSE BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI- FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 14 X 8 X 4 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- RED, GRAY AND FIRM FOCAL -B-ADENOMA,-NEOPLASM PRESENT 4681 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 513 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92393 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/18/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 517.2 GRAMS DATE AND TIME OF NECROPSY: 01/18/00 10:44 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 3 MM IN DIAMETER DEATH COMMENT (DC) : -MAMMARY FIBROADENOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS 6 X 4 X 2 -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MM; COLLECTED MULTI-FOCAL LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS 10 X 7 X 3 -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MM (CAP 18) MULTI-FOCAL -DIFFUSELY RED; LEFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 4682 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 514 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92393 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/18/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 517.2 GRAMS DATE AND TIME OF NECROPSY: 01/18/00 10:44 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4683 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 515 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92394 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 375.9 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 14:27 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL; WEEK 36; KIDNEY (KD) : 75 X 50MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-SLIGHT, MULTI- OBSERVED FOCAL >DISCHARGE; NASAL; RED IN COLOR; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -DEPRESSED FOCUS(I)/AREA(S); CAUDATE -CYST, BILIARY,-MODERATE, FOCAL >APPEARANCE; THIN; VERIFIED AT LOBE: MULTIPLE DARK BROWN SLIGHTLY -TELANGIECTASIS,-SEVERE, FOCAL NECROPSY: OBSERVED DEPRESSED AREAS, UP TO 5 X 4 X 2 MM (CAP 19) MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT CERVICAL REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL SINGLE MASS, 75 X 50 X 35 MM, RED, -M-CARCINOMA,-NEOPLASM PRESENT DARK RED, TAN, LOBULATED, AND VASCULAR; ON SECTION EXUDES A DARK BROWN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 18) BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 7 X 6 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, DARK RED, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4684 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 516 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92394 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 375.9 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 14:27 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; BILATERAL: DARK RED, DRY, CRUSTED MATERIAL -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4685 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 517 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92395 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 390.5 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:01 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0542 .0139 % .0214 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0419 .0107 % .0166 WEIGHT TAKEN BRAIN (BR) 2.5272 .6472 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.4087 .3607 % .5574 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.3994 .3584 % .5537 WEIGHT TAKEN LIVER (LI) 11.0859 2.8389 % 4.3866 WEIGHT TAKEN LUNG (LU) 1.8104 .4636 % .7164 WEIGHT TAKEN LEFT OVARY (OV0) .0517 .0132 % .0205 WEIGHT TAKEN RIGHT OVARY (OV1) .0567 .0145 % .0224 WEIGHT TAKEN SPLEEN (SP) .5787 .1482 % .2290 WEIGHT TAKEN LF THYROID/PARA (TT0) .0128 .0033 % .0051 WEIGHT TAKEN RT THYROID/PARA (TT1) .0124 .0032 % .0049 WEIGHT TAKEN UTERUS (UT) 1.0112 .2590 % .4001 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LUNG (LU) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, FOCAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 8 X 6 X 4 MM >UNREMARKABLE HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL RECTUM (RE) : -PARASITISM,-PRESENT 4686 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 518 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92395 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 390.5 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:01 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4687 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 519 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92396 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 275.2 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:30 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.6550 2.7816 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : VERIFIED AT NECROPSY: OBSERVED -NO MACROSCOPIC LESIONS -------------------------------------------- 4688 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 520 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92396 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 275.2 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:30 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4689 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 521 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92397 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 273.1 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:43 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 8.5809 3.1420 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; DISPLACED PUPIL; EYE-RIGHT; GENERAL COMMENT (GC) : VERIFIED AT NECROPSY: NOT APPLICABLE -NO MACROSCOPIC LESIONS -------------------------------------------- 4690 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 522 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92397 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 273.1 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:43 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4691 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 523 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92398 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 287.0 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:19 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.7048 2.6846 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : VERIFIED AT NECROPSY: OBSERVED -NO MACROSCOPIC LESIONS -------------------------------------------- 4692 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 524 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92398 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 287.0 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:19 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4693 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 525 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92399 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 242.0 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:30 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 8.0109 3.3103 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : VERIFIED AT NECROPSY: OBSERVED -NO MACROSCOPIC LESIONS -------------------------------------------- 4694 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 526 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92399 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 242.0 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:30 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4695 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 527 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92400 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 276.4 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:01 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 8.5181 3.0818 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT GENERAL COMMENT (GC) : NECROPSY: NOT APPLICABLE -NO MACROSCOPIC LESIONS -------------------------------------------- 4696 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 528 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92400 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 276.4 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:01 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 4697 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 529 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92401 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/06/00 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 355.1 GRAMS DATE AND TIME OF NECROPSY: 01/06/00 15:38 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HEAD TILT; LEFT; VERIFIED KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MODERATE, >APPEARANCE; THIN; VERIFIED AT FOCAL NECROPSY: NOT OBSERVED MAMMARY, FEMALE (MF) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -GALACTOCELE,-MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE -HYPERPLASIA, GLANDULAR,-MODERATE, >APPEARANCE; MALOCCLUSION; VERIFIED AT DIFFUSE NECROPSY: OBSERVED BRAIN (BR) : BRAIN (BR) : >SKIN & PELAGE; PALE; ENTIRE BODY; -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE -DILATATION, VENTRICULAR,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT PITUITARY (PI) : PITUITARY (PI) : NECROPSY: OBSERVED -MASS(ES); 12 X 10 X 8 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE CLEAR DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -DISCHARGE-OCULAR; BILATERAL: RED -------------------------------------------- 4698 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 530 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92401 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/06/00 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 355.1 GRAMS DATE AND TIME OF NECROPSY: 01/06/00 15:38 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 4699 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 531 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92402 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 200.5 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:24 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.3283 3.6550 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : UNVERIFIED AT NECROPSY -NO MACROSCOPIC LESIONS -------------------------------------------- 4700 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 532 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92402 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 200.5 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:24 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) ------------------------------------------------------------------------------------------------------------------------------------ 4701 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 533 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92403 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/27/99 STUDY DAY OF DEATH: 356 STUDY WEEK OF DEATH: 51 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/27/99 15:17 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL; WEEK 35; KIDNEY (KD) : 10 X 10MM; VERIFIED AT NECROPSY: NOT -MINERALIZATION, PELVIC,-SLIGHT, MULTI- OBSERVED FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -HYPERPLASIA, GLANDULAR,-SLIGHT, FOCAL HEART (HT) : HEART (HT) : -LIGHT FOCUS(I)/AREA(S); DIFFUSE, -FIBROSIS,-MODERATE, MULTI-FOCAL EPICARDIUM: MULTIPLE GRAY INDISTINCT -INFLAMMATION, CHRONIC,-MODERATE, MULTI- AREAS FOCAL DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -DIFFUSELY RED; DARK RED (CAP 18) -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -------------------------------------------- 4702 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 534 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92403 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/27/99 STUDY DAY OF DEATH: 356 STUDY WEEK OF DEATH: 51 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/27/99 15:17 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 4703 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 535 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92404 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 566 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 453.4 GRAMS DATE AND TIME OF NECROPSY: 10/23/99 10:30 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK LUNG (LU) : 70; 25 X 25MM; VERIFIED AT NECROPSY: -MINERALIZATION, VASCULAR,-MINIMAL, OBSERVED FOCAL >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK KIDNEY (KD) : 76; 50 X 45MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MODERATELY OBSERVED SEVERE, MULTI-FOCAL >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; LIVER (LI) : WEEK 79; VERIFIED AT NECROPSY: NOT -FOCUS, ALTERED HEPATOCELLULAR, OBSERVED BASOPHILIC,-MODERATE, MULTI-FOCAL >SKIN & PELAGE; SORE/SCAB; VENTRAL- -VACUOLATION, HEPATOCELLULAR,-MINIMAL, CERVICAL-LEFT; VERIFIED AT NECROPSY: MULTI-FOCAL OBSERVED PANCREAS (PA) : >SKIN & PELAGE; YELLOW HAIR COAT; -ATROPHY, ACINAR,-MINIMAL, FOCAL VERIFIED AT NECROPSY: OBSERVED SKIN (SK) : >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -FIBROSIS,-MODERATELY SEVERE, FOCAL AT NECROPSY: NOT APPLICABLE -PIGMENT,-MODERATE, MULTI-FOCAL -M-CARCINOMA, SQUAMOUS CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,-NEOPLASM PRESENT 4704 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 536 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92404 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 566 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 453.4 GRAMS DATE AND TIME OF NECROPSY: 10/23/99 10:30 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); LEFT LATERAL CERVICAL REGION: SINGLE MASS, 20 MM IN DIAMETER, RED, DARK RED, TAN, BROWN, SOFT, AND LOBULATED; SAME ON CUT SURFACE; OVERLYING SKIN HAS A DARK RED, DRY, CRUSTED AREA, 10 MM IN DIAMETER (CAP 21); REGIONAL LN (SEE MANDIBULAR) -THICKENED; ALL REGIONS, DIFFUSE: SOME AREAS ARE TAN, FIRM, AND LOBULATED (CAP 20) -MASS #2; LEFT LATERAL THORACIC AND LUMBAR REGIONS: SINGLE MASS, 85 X 60 X 30 MM, RED, DARK RED, TAN, BROWN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; ON SECTION, SOME AREAS EXUDED A DARK RED SEMIFLUID; COLLECTED; REGIONAL LN (SEE AXILLARY) BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 5 MM, RED, GRAY, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WHITE AND MUCOID >UNREMARKABLE DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4705 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 537 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92404 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 566 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 453.4 GRAMS DATE AND TIME OF NECROPSY: 10/23/99 10:30 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: BROWN AND TAN >UNREMARKABLE (CAP 19) LN, AXILLARY (AX) : LN, AXILLARY (AX) : -DIFFUSELY RED; LEFT: DARK RED (CAP -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 18) -INFILTRATE, MACROPHAGE, VACUOLATED,- -MOTTLED; RIGHT: DARK BROWN AND TAN SLIGHT, MULTI-FOCAL (CAP 18) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST -------------------------------------------- 4706 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 538 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92404 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 566 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 453.4 GRAMS DATE AND TIME OF NECROPSY: 10/23/99 10:30 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 4707 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 539 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92405 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/20/00 STUDY DAY OF DEATH: 686 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 364.2 GRAMS DATE AND TIME OF NECROPSY: 02/20/00 7:53 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; BROWN HAIR COAT; KIDNEY (KD) : PERINEAL AREA; VERIFIED AT NECROPSY: -CAST, PROTEINACEOUS,-MINIMAL, MULTI- NOT OBSERVED FOCAL >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -MINERALIZATION, PELVIC,-MINIMAL, MULTI- AT NECROPSY: NOT APPLICABLE FOCAL >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- >BEHAVIOR; HYPOACTIVE; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATELY SEVERE, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 12 X 9 X 6 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, DARK RED, TAN, GRAY, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT CECUM (CE) : CECUM (CE) : -THICKENED WALL; DIFFUSE -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO -MASS(ES); SINGLE MASS, 35 X 22 X 12 NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MM, RED, AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT WITH CECUM; REGIONAL LN (MESENTERIC) NORMAL DEATH COMMENT (DC) : -MALIGNANT LYMPHOMA,-PRESENT 4708 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 540 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92405 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/20/00 STUDY DAY OF DEATH: 686 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 364.2 GRAMS DATE AND TIME OF NECROPSY: 02/20/00 7:53 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), LN, MESENTERIC (MS) ------------------------------------------------------------------------------------------------------------------------------------ 4709 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 541 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92406 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 331.3 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:53 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0465 .0140 % .0223 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0317 .0096 % .0152 WEIGHT TAKEN BRAIN (BR) 2.0870 .6299 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.3016 .3929 % .6237 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.3809 .4168 % .6617 WEIGHT TAKEN LIVER (LI) 9.2698 2.7980 % 4.4417 WEIGHT TAKEN LUNG (LU) 1.2827 .3872 % .6146 WEIGHT TAKEN LEFT OVARY (OV0) .0475 .0143 % .0228 WEIGHT TAKEN RIGHT OVARY (OV1) .0543 .0164 % .0260 WEIGHT TAKEN SPLEEN (SP) .4773 .1441 % .2287 WEIGHT TAKEN LF THYROID/PARA (TT0) .0105 .0032 % .0050 WEIGHT TAKEN RT THYROID/PARA (TT1) .0107 .0032 % .0051 WEIGHT TAKEN UTERUS (UT) 1.3733 .4145 % .6580 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LUNG (LU) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL KIDNEY (KD) : KIDNEY (KD) : -CYST(S); LEFT ON CORTICAL SURFACE, -CYST,-MODERATE, FOCAL EXTENDS INTO MEDULLA: SINGLE CLEAR -HYPERPLASIA, TRANSITIONAL CELL,- FLUID FILLED CYST, 6 MM IN DIAMETER SLIGHT, DIFFUSE -INFLAMMATION, CHRONIC,-MODERATE, FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN 4710 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 542 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92406 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 331.3 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:53 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY), MAMMARY, FEMALE (MF) ------------------------------------------------------------------------------------------------------------------------------------ 4711 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 543 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92407 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 490.3 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:05 PROSECTOR: MONTI THOMAS MAGUIRE RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0454 .0093 % .0225 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0437 .0089 % .0216 WEIGHT TAKEN BRAIN (BR) 2.0210 .4122 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.4233 .2903 % .7043 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.5123 .3084 % .7483 WEIGHT TAKEN LIVER (LI) 13.2832 2.7092 % 6.5726 WEIGHT TAKEN LUNG (LU) 1.4892 .3037 % .7369 WEIGHT TAKEN LEFT OVARY (OV0) .0476 .0097 % .0236 WEIGHT TAKEN RIGHT OVARY (OV1) .0541 .0110 % .0268 WEIGHT TAKEN SPLEEN (SP) .4835 .0986 % .2392 WEIGHT TAKEN LF THYROID/PARA (TT0) .0241 .0049 % .0119 WEIGHT TAKEN RT THYROID/PARA (TT1) .0211 .0043 % .0104 WEIGHT TAKEN UTERUS (UT) 1.1172 .2279 % .5528 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MINIMAL, FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL; COLLECTED -HEMORRHAGE,-SLIGHT, MULTI-FOCAL GENERAL COMMENT (GC) : -ANIMAL OBESE -BONE MARROW SMEAR TAKEN 4712 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 544 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92407 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 490.3 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:05 PROSECTOR: MONTI THOMAS MAGUIRE RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), MAMMARY, FEMALE (MF) ------------------------------------------------------------------------------------------------------------------------------------ 4713 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 545 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92408 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 431.7 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 14:27 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK LUNG (LU) : 73; 100 X 85MM; VERIFIED AT NECROPSY: -INFILTRATE, MACROPHAGE, ALVEOLAR,- OBSERVED SLIGHT, FOCAL >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; KIDNEY (KD) : WEEK 92; 35 X 20MM; VERIFIED AT -MINERALIZATION, PELVIC,-SLIGHT, FOCAL NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EYES; PALE; EYES; VERIFIED AT MINIMAL, MULTI-FOCAL NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : -LARGE; ALL LOBES: MARGINS APPEAR -FOCUS, ALTERED HEPATOCELLULAR, ROUNDED EOSINOPHILIC,-MODERATE, MULTI-FOCAL -RAISED FOCUS(I)/AREA(S); -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM DIAPHRAGMATIC SURFACE OF LEFT LATERAL PRESENT LOBE: SINGLE AREA, 10 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 90 X 60 X 45 MM, RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL DARK RED, TAN, VASCULAR, LOBULATED AND -B-FIBROADENOMA,-NEOPLASM PRESENT FIRM; SAME ON CUT SURFACE; PORTION -M-CARCINOMA,-NEOPLASM PRESENT COLLECTED; REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS: DIFFUSE; COLLECTED ON ROUTINE SECTION 4714 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 546 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92408 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 431.7 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 14:27 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 30 X 25 X 15 MM, RED, TAN, VASCULAR, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, DARK RED -HEMORRHAGE,-MODERATE, MULTI-FOCAL AND SOFT -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; RIGHT: 12 X 7 X 3 MM (LEFT IS >UNREMARKABLE 3 X 2 X 1 MM) (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 4715 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 547 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92408 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 431.7 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 14:27 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA) ------------------------------------------------------------------------------------------------------------------------------------ 4716 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 548 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92409 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 376.1 GRAMS DATE AND TIME OF NECROPSY: 02/25/00 10:25 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK KIDNEY (KD) : 74; 40 X 40MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MODERATE, OBSERVED MULTI-FOCAL >EYES; RED DISCHARGE; EYES; VERIFIED AT LIVER (LI) : NECROPSY: NOT OBSERVED -VACUOLATION, HEPATOCELLULAR,-SLIGHT, >SKIN & PELAGE; RED HAIR COAT; HEAD- MULTI-FOCAL ENTIRE; VERIFIED AT NECROPSY: NOT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : OBSERVED -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL >APPEARANCE; HEAD TILT; RIGHT; VERIFIED SINGLE MASS, 50 X 40 X 25 MM, RED, -PIGMENT,-MODERATELY SEVERE, FOCAL AT NECROPSY: NOT APPLICABLE DARK RED, TAN, FIRM, AND LOBULATED; -B-FIBROADENOMA,- 4 NEOPLASMS PRESENT >BEHAVIOR; ATAXIC; VERIFIED AT SAME ON CUT SURFACE; ON SECTION, SOME NECROPSY: NOT APPLICABLE AREAS EXUDE A TAN CREAMY SEMIFLUID; >BEHAVIOR; HYPOACTIVE; VERIFIED AT COLLECTED; REGIONAL LN (AXILLARY) NECROPSY: NOT APPLICABLE NORMAL (CAP 21) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER (CAP 25) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM (CAP 24) -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 20 X 10 X 3 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 21) 4717 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 549 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92409 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 376.1 GRAMS DATE AND TIME OF NECROPSY: 02/25/00 10:25 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #3; RIGHT INGUINAL REGION: SINGLE MASS, 15 X 15 X 8 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 26); REGIONAL LN (INGUINAL) NOT IDENTIFIED BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 12 X 8 X 7 MM (RIGHT IS -DEGENERATION, CYSTIC, CORTICAL,- 4 X 3 X 3 MM) SEVERE, FOCAL -MOTTLED; LEFT: RED AND BROWN -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 7 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, BROWN, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT CECUM (CE) : CECUM (CE) : -THICKENED WALL; DIFFUSE >UNREMARKABLE UTERUS (UT) : UTERUS (UT) : -MASS(ES); ATTACHED TO ENDOMETRIUM OF -DILATATION,-MODERATELY SEVERE, FOCAL RIGHT MIDDLE HORN: SINGLE POLYPOID -B-POLYP, ENDOMETRIAL, STROMAL,- MASS, 18 X 12 X 9 MM, RED, DARK RED, NEOPLASM PRESENT SOFT, AND GELATINOUS; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 27) VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; RED SEMIFLUID >UNREMARKABLE DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4718 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 550 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92409 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 376.1 GRAMS DATE AND TIME OF NECROPSY: 02/25/00 10:25 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 4719 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 551 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92410 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 450.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:48 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL; WEEK 88; LUNG (LU) : 40 X 35MM; VERIFIED AT NECROPSY: -MINERALIZATION, VASCULAR,-MINIMAL, OBSERVED MULTI-FOCAL >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK KIDNEY (KD) : 103; 10 X 10MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MODERATE, OBSERVED MULTI-FOCAL LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); RIGHT MEDIAN -FOCUS, ALTERED HEPATOCELLULAR, LOBE: SINGLE RED FOCUS, 3 MM IN BASOPHILIC,-SLIGHT, MULTI-FOCAL DIAMETER; COLLECTED ON ROUTINE SECTION -TELANGIECTASIS,-MINIMAL, FOCAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT, FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL SINGLE MASS, 15 X 10 X 6 MM, RED, TAN -MINERALIZATION,-MINIMAL, MULTI-FOCAL AND FIRM; SAME ON CUT SURFACE (CAP -M-CARCINOMA,- 2 NEOPLASMS PRESENT 22); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT CERVICAL REGION: SINGLE MASS, 40 X 20 X 20 MM, RED, DARK RED, TAN AND SOFT; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A DARK RED SEMIFLUID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 23) 4720 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 552 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92410 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 450.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:48 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 4 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), URINARY BLADDER (UB), LN, MANDIBULAR (MN) ------------------------------------------------------------------------------------------------------------------------------------ 4721 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 553 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92411 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 392.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 11:10 PROSECTOR: MONTI THOMAS MAGUIRE RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0602 .0153 % .0272 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0424 .0108 % .0191 WEIGHT TAKEN BRAIN (BR) 2.2159 .5647 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.5354 .3913 % .6929 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.7134 .4366 % .7732 WEIGHT TAKEN LIVER (LI) 12.1056 3.0850 % 5.4631 WEIGHT TAKEN LUNG (LU) 1.6581 .4226 % .7483 WEIGHT TAKEN LEFT OVARY (OV0) .0873 .0222 % .0394 WEIGHT TAKEN RIGHT OVARY (OV1) .0786 .0200 % .0355 WEIGHT TAKEN SPLEEN (SP) .5287 .1347 % .2386 WEIGHT TAKEN LF THYROID/PARA (TT0) .0150 .0038 % .0068 WEIGHT TAKEN RT THYROID/PARA (TT1) .0163 .0042 % .0074 WEIGHT TAKEN UTERUS (UT) 1.3063 .3329 % .5895 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : 44; VERIFIED AT NECROPSY: OBSERVED -MASS(ES); LEFT AXILLARY REGION: -GALACTOCELE,-MODERATE, FOCAL >APPEARANCE; MALOCCLUSION; VERIFIED AT SINGLE MASS, 15 X 10 X 5 MM, RED, TAN -MINERALIZATION,-MINIMAL, MULTI-FOCAL NECROPSY: OBSERVED AND FIRM; SAME ON CUT SURFACE (CAP -B-FIBROADENOMA,-NEOPLASM PRESENT 18); REGIONAL LN (AXILLARY) NORMAL (CAP 19) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE WHITE CREAMY SEMIFLUID FILLED CYSTS, UP TO 8 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4722 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 554 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92411 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 392.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 11:10 PROSECTOR: MONTI THOMAS MAGUIRE RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND DARK RED; >UNREMARKABLE COLLECTED GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL UPPER INCISORS -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 4723 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 555 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92412 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 465.8 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:42 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); ANTERIOR -HEMORRHAGE,-MODERATELY SEVERE, FOCAL ASPECT: SINGLE DARK RED AND RAISED -B-ADENOMA,-NEOPLASM PRESENT AREA, 3 MM IN DIAMETER OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 6 MM IN DIAMETER DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4724 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 556 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92412 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 465.8 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:42 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 4725 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 557 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92413 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/15/00 STUDY DAY OF DEATH: 710 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 456.5 GRAMS DATE AND TIME OF NECROPSY: 03/15/00 15:09 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LUNG (LU) : NECROPSY: OBSERVED -MINERALIZATION, VASCULAR,-MINIMAL, >SKIN & PELAGE; SORE/SCAB; PAW-HIND- FOCAL RIGHT; VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : >MASS(ES); AXILLARY REGION-RIGHT; WEEK -MINERALIZATION, PELVIC,-MINIMAL, MULTI- 96; 55 X 50MM; VERIFIED AT NECROPSY: FOCAL NOT OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >APPEARANCE; HEAD TILT; RIGHT; VERIFIED MODERATE, DIFFUSE AT NECROPSY: NOT APPLICABLE -PYELONEPHRITIS,-MINIMAL, MULTI-FOCAL >RESPIRATION; LABORED; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, >EXCRETION; FEW FECES; VERIFIED AT EOSINOPHILIC,-SLIGHT, FOCAL NECROPSY: NOT APPLICABLE THYROID (TY) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -HYPERPLASIA, C-CELL,-MINIMAL, FOCAL NECROPSY: NOT APPLICABLE MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 8 X 6 X 5 MM (RIGHT IS 6 -ANGIECTASIS,-MODERATE, MULTI-FOCAL X 3 X 3 MM) -DEGENERATION, CYSTIC, CORTICAL,- MODERATE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL 4726 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 558 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92413 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/15/00 STUDY DAY OF DEATH: 710 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 456.5 GRAMS DATE AND TIME OF NECROPSY: 03/15/00 15:09 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 7 X 6 MM, DARK RED, -CYST,-PRESENT RED, TAN AND SOFT -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: -FIBROSIS,-MODERATE, FOCAL SINGLE RED, TAN AND DRY AREA, 3 MM IN DIAMETER; COLLECTED ADIPOSE TISSUE (AT) : ADIPOSE TISSUE (AT) : -THICKENED; RIGHT AXILLARY REGION: -B-LIPOMA,-NEOPLASM PRESENT SINGLE TAN AND FIRM AREA, 60 X 50 X 20 MM; COLLECTED; POSSIBLY CONSISTENT WITH MASS NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN AT BASE -------------------------------------------- 4727 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 559 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92413 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/15/00 STUDY DAY OF DEATH: 710 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 456.5 GRAMS DATE AND TIME OF NECROPSY: 03/15/00 15:09 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA) ------------------------------------------------------------------------------------------------------------------------------------ 4728 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 560 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92414 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/14/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 513.7 GRAMS DATE AND TIME OF NECROPSY: 06/14/99 14:08 PROSECTOR: TODD ACKER RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; KIDNEY (KD) : WEEK 44; 100 X 80MM; VERIFIED AT -MINERALIZATION, PELVIC,-SLIGHT, MULTI- NECROPSY: OBSERVED FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 120 X 80 X 50 MM (173.8 GRAMS), RED, DARK RED, TAN, VASCULAR AND SOFT; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MULTIPLE CLEAR FLUID FILLED CYSTS, UP MODERATE, FOCAL TO 4 MM IN DIAMETER DEATH COMMENT (DC) : -MAMMARY FIBROADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 4729 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 561 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92414 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/14/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 513.7 GRAMS DATE AND TIME OF NECROPSY: 06/14/99 14:08 PROSECTOR: TODD ACKER RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI) ------------------------------------------------------------------------------------------------------------------------------------ 4730 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 562 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92415 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 619 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/15/99 11:24 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LUNG (LU) : LUNG (LU) : VERIFIED AT NECROPSY: NOT OBSERVED -UNCOLLAPSED; ALL LOBES -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MODERATELY SEVERE, MULTI-FOCAL LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL, FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, MULTI-FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); LEFT INGUINAL REGION: SINGLE -GALACTOCELE,-MODERATELY SEVERE, MULTI- TAN, CREAMY SEMIFLUID FILLED CYST, 25 FOCAL X 25 X 5 MM; TISSUE SURROUNDING IS -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, THICKENED, TAN AND FIRM (CAP 18) MULTI-FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 9 X 7 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GREY, LOBULATED AND SOFT OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 3 MM IN DIAMETER 4731 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 563 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92415 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 619 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/15/99 11:24 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 4732 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 564 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92416 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 416.9 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:30 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); TAIL-PROXIMAL; WEEK 75; 20 X KIDNEY (KD) : 15MM; VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-SLIGHT, MULTI- >WART LIKE LESION(S); HEAD-MAXILLARY- FOCAL RIGHT; WEEK 79; VERIFIED AT NECROPSY: THYROID (TY) : OBSERVED -HYPERPLASIA, C-CELL,-SLIGHT, FOCAL >SKIN & PELAGE; RED HAIR COAT; HEAD- SKIN (SK) : SKIN (SK) : CRANIAL; VERIFIED AT NECROPSY: -CRUSTED AREA(S); RIGHT EYELID: SINGLE -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM OBSERVED DRY, TAN CRUSTED AREA, 6 X 4 MM (CAP PRESENT >SKIN & PELAGE; SORE/SCAB; TAIL; 22) VERIFIED AT NECROPSY: OBSERVED DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT TAIL (TI) : TAIL (TI) : -ULCERATION(S)/ABRASION(S); 25 MM FROM -EROSION,-MODERATE, FOCAL BASE OF TAIL: SINGLE TAN, RED, MOIST, -INFLAMMATION, ACUTE,-SLIGHT, FOCAL RAISED ULCERATED AREA, 22 X 19 X 14 -M-FIBROSARCOMA,-NEOPLASM PRESENT MM; COLLECTED; POSSIBLY CONSISTENT WITH MASS (TAIL) AS NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: DRY RED MATERIAL -------------------------------------------- 4733 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 565 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92416 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 416.9 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:30 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), MAMMARY, FEMALE (MF), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 4734 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 566 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92417 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/18/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 237.7 GRAMS DATE AND TIME OF NECROPSY: 01/18/00 10:16 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT LUNG (LU) : NECROPSY: NOT APPLICABLE -HEMORRHAGE,-MINIMAL, MULTI-FOCAL >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-SLIGHT, MULTI- >SKIN & PELAGE; YELLOW HAIR COAT; FOCAL VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MODERATE, >APPEARANCE; HEAD TILT; RIGHT; VERIFIED MULTI-FOCAL AT NECROPSY: NOT APPLICABLE LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 7 MM IN DIAMETER (LEFT -DEGENERATION, CYSTIC, CORTICAL,- IS 4 MM IN DIAMETER) MODERATELY SEVERE, FOCAL -MOTTLED; RIGHT: BROWN AND RED -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -VACUOLATION,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 6 MM, DARK RED, -CYST,-PRESENT LOBULATED AND FIRM -HEMORRHAGE,-MODERATE, MULTI-FOCAL 4735 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 567 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92417 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/18/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 237.7 GRAMS DATE AND TIME OF NECROPSY: 01/18/00 10:16 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 6 MM IN DIAMETER DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 4736 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 568 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92417 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/18/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 237.7 GRAMS DATE AND TIME OF NECROPSY: 01/18/00 10:16 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 4737 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 569 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92418 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 427.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 11:10 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0407 .0095 % .0201 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0289 .0068 % .0143 WEIGHT TAKEN BRAIN (BR) 2.0241 .4740 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.2464 .2919 % .6158 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.2915 .3025 % .6381 WEIGHT TAKEN LIVER (LI) 11.4976 2.6926 % 5.6804 WEIGHT TAKEN LUNG (LU) 1.4056 .3292 % .6944 WEIGHT TAKEN LEFT OVARY (OV0) .0639 .0150 % .0316 WEIGHT TAKEN RIGHT OVARY (OV1) .0487 .0114 % .0241 WEIGHT TAKEN SPLEEN (SP) .5717 .1339 % .2824 WEIGHT TAKEN LF THYROID/PARA (TT0) .0150 .0035 % .0074 WEIGHT TAKEN RT THYROID/PARA (TT1) .0113 .0026 % .0056 WEIGHT TAKEN UTERUS (UT) 1.0721 .2511 % .5297 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; DEATH COMMENT (DC) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 4738 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 570 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92418 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 427.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 11:10 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), MAMMARY, FEMALE (MF) ------------------------------------------------------------------------------------------------------------------------------------ 4739 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 571 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92419 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 402.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:32 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >MASS(ES); AXILLARY REGION-LEFT; WEEK MINIMAL, MULTI-FOCAL 96; VERIFIED AT NECROPSY: OBSERVED -PYELONEPHRITIS,-MINIMAL, MULTI-FOCAL >MASS(ES); AXILLARY REGION-RIGHT; WEEK LIVER (LI) : LIVER (LI) : 101; 25 X 25MM; VERIFIED AT NECROPSY: -CYST(S); DIAPHRAGMATIC SURFACE OF -DEGENERATION, CYSTIC,-MODERATE, FOCAL OBSERVED RIGHT MEDIAN LOBE: SINGLE CLEAR FLUID -FOCUS, ALTERED HEPATOCELLULAR, FILLED CYST, 1 MM IN DIAMETER; EOSINOPHILIC,-MODERATE, MULTI-FOCAL COLLECTED ON ROUTINE SECTION MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL SINGLE MASS, 16 X 8 X 8 MM, TAN, DARK -B-ADENOMA,-NEOPLASM PRESENT BROWN, FIRM AND LOBULATED (CAP 22); -B-FIBROADENOMA,-NEOPLASM PRESENT SAME ON CUT SURFACE; REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 35 X 25 X 21 MM, RED, TAN, FIRM AND LOBULATED; ON SECTION EXUDES A TAN CREAMY SEMISOLID MATERIAL; COLLECTED; REGIONAL LN (SEE AXILLARY) BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT 4740 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 572 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92419 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 402.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:32 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 6 MM IN DIAMETER (LEFT -DEGENERATION, CYSTIC, CORTICAL,- IS 3 MM IN DIAMETER) MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 4 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- GRAY, SOFT AND LOBULATED FOCAL -PIGMENT,-MINIMAL, FOCAL -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : UTERUS (UT) : -CYST(S); WITHIN WALL OF RIGHT HORN: -DILATATION,-MODERATE, DIFFUSE MULTIPLE CLEAR FLUID FILLED CYSTS, UP -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- TO 7 X 6 X 6 MM MODERATELY SEVERE, MULTI-FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: DARK RED AND -HEMORRHAGE,-MINIMAL, MULTI-FOCAL BROWN (CAP 23) -INFILTRATE, MACROPHAGE, VACUOLATED,- MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL LOWER INCISORS -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; RIGHT UPPER INCISOR: BROKEN OFF AT BASE -------------------------------------------- 4741 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 573 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92419 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 402.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:32 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 4742 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 574 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92420 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 505 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 569.1 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 13:40 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK LIVER (LI) : 59; 55 X 60MM; VERIFIED AT NECROPSY: -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- OBSERVED FOCAL >SKIN & PELAGE; SORE/SCAB; AXILLARY MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : REGION-RIGHT; VERIFIED AT NECROPSY: -MASS(ES); RIGHT SHOULDER REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL OBSERVED SINGLE MASS, 68 X 51 X 43 MM, RED, -HEMORRHAGE,-MODERATE, FOCAL DARK RED, TAN, FIRM AND LOBULATED; -INFLAMMATION, ACUTE,-SLIGHT, MULTI- SAME ON CUT SURFACE; OVERLYING SKIN FOCAL HAS A SINGLE DRY RED CRUSTED AREA, 9 X -PIGMENT,-SLIGHT, FOCAL 4 MM; COLLECTED INTACT; POSSIBLY -M-CARCINOMA,-NEOPLASM PRESENT CONSISTENT WITH SCAB (RIGHT AXILLARY) AS NOTED IN CLINICAL OBSERVATIONS; REGIONAL LN (AXILLARY) NORMAL (CAP 18) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 6 X 4 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN LUMEN: -DILATATION,-MODERATELY SEVERE, FOCAL MULTIPLE CLEAR FLUID FILLED CYSTS, UP -B-POLYP, ENDOMETRIAL, STROMAL,- TO 5 MM IN DIAMETER NEOPLASM PRESENT 4743 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 575 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92420 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 505 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 569.1 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 13:40 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : -MASS(ES); RIGHT HORN, WITHIN LUMEN, ATTACHED TO WALL: SINGLE MASS, 19 X 6 X 6 MM, TAN AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 19) DEATH COMMENT (DC) : -MAMMARY CARCINOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), LN, AXILLARY (AX), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 4744 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 576 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92421 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 446.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 12:51 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LUNG (LU) : LUNG (LU) : NECROPSY: NOT OBSERVED -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, MACROPHAGE, ALVEOLAR,- >SKIN & PELAGE; SORE/SCAB; VENTRAL- MULTIPLE TAN AREAS, UP TO 4 X 3 MM MODERATE, MULTI-FOCAL ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: KIDNEY (KD) : OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); MID PERIANAL REGION: -INFLAMMATION, CHRONIC-ACTIVE,- SINGLE DRY, TAN CRUSTED AREA, 7 MM IN MODERATE, MULTI-FOCAL DIAMETER (CAP 22) -B-KERATOACANTHOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 8 X 5 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, AND FIRM DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4745 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 577 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92421 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 446.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 12:51 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS 11 X 7 X 4 -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MM (CAP 23) MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 4746 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 578 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92422 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 405.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:56 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0352 .0087 % .0164 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0282 .0070 % .0131 WEIGHT TAKEN BRAIN (BR) 2.1483 .5299 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.1812 .2914 % .5498 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.1924 .2941 % .5550 WEIGHT TAKEN LIVER (LI) 8.9117 2.1982 % 4.1483 WEIGHT TAKEN LUNG (LU) 1.4161 .3493 % .6592 WEIGHT TAKEN LEFT OVARY (OV0) .0726 .0179 % .0338 WEIGHT TAKEN RIGHT OVARY (OV1) .0724 .0179 % .0337 WEIGHT TAKEN SPLEEN (SP) .5826 .1437 % .2712 WEIGHT TAKEN LF THYROID/PARA (TT0) .0173 .0043 % .0081 WEIGHT TAKEN RT THYROID/PARA (TT1) .0231 .0057 % .0108 WEIGHT TAKEN UTERUS (UT) .5629 .1389 % .2620 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; DEATH COMMENT (DC) : VERIFIED AT NECROPSY: OBSERVED -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 4747 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 579 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92422 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 405.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:56 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), MAMMARY, FEMALE (MF) ------------------------------------------------------------------------------------------------------------------------------------ 4748 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 580 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92423 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 566.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:33 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : PARATHYROID (PT) : -LARGE; RIGHT: 4 X 3 X 2 MM (LEFT IS 1 >UNREMARKABLE MM IN DIAMETER) MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 5 X 4 MM, RED, DARK -CYST,-PRESENT RED, GRAY AND FIRM -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID >UNREMARKABLE FILLED CYST, 2 MM IN DIAMETER 4749 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 581 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92423 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 566.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:33 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 4750 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 582 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92424 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 399.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:03 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0428 .0107 % .0215 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0470 .0118 % .0236 WEIGHT TAKEN BRAIN (BR) 1.9919 .4987 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.3161 .3295 % .6607 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.3429 .3362 % .6742 WEIGHT TAKEN LIVER (LI) 10.7548 2.6927 % 5.3993 WEIGHT TAKEN LUNG (LU) 1.5499 .3881 % .7781 WEIGHT TAKEN LEFT OVARY (OV0) .0701 .0176 % .0352 WEIGHT TAKEN RIGHT OVARY (OV1) .0572 .0143 % .0287 WEIGHT TAKEN SPLEEN (SP) .5224 .1308 % .2623 WEIGHT TAKEN LF THYROID/PARA (TT0) .0323 .0081 % .0162 WEIGHT TAKEN RT THYROID/PARA (TT1) .0121 .0030 % .0061 WEIGHT TAKEN UTERUS (UT) 1.3871 .3473 % .6964 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; THYROID (TY) : VERIFIED AT NECROPSY: OBSERVED -CYST, FOLLICULAR,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL UTERUS (UT) : UTERUS (UT) : -CYST(S); WITHIN WALL OF RIGHT HORN: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- SINGLE CLEAR FLUID FILLED CYST, 15 X 5 MODERATE, FOCAL X 5 MM DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN 4751 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 583 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92424 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 399.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:03 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA) ------------------------------------------------------------------------------------------------------------------------------------ 4752 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 584 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92425 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 555.1 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 13:50 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-RIGHT; WEEK KIDNEY (KD) : 84; 50 X 50MM; VERIFIED AT NECROPSY: -CAST, PROTEINACEOUS,-MINIMAL, MULTI- OBSERVED FOCAL >MASS(ES); INGUINAL REGION-LEFT; WEEK LIVER (LI) : 92; 30 X 25MM; VERIFIED AT NECROPSY: -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT, OBSERVED MULTI-FOCAL >SKIN & PELAGE; SORE/SCAB; VENTRAL- THYROID (TY) : ABDOMINAL-LEFT; VERIFIED AT NECROPSY: -CYST, ULTIMOBRANCHIAL,-PRESENT OBSERVED MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL SINGLE MASS, 50 X 30 X 25 MM, RED, -MINERALIZATION,-MINIMAL, MULTI-FOCAL TAN, FIRM, AND LOBULATED; SOME -B-ADENOMA,-NEOPLASM PRESENT SECTIONS EXUDE A TAN CREAMY SEMIFLUID; -M-CARCINOMA,-NEOPLASM PRESENT COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT PERIANAL REGION: SINGLE MASS, 45 X 30 X 15 MM, RED, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN HAS A SINGLE RED AND TAN AREA, EXTENDING INTO LEFT INGUINAL REGION, 50 X 30 MM; COLLECTED WITH MASS BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT 4753 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 585 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92425 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 555.1 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 13:50 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SPLEEN (SP) : SPLEEN (SP) : -LARGE; 52 X 12 X 8 MM -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 5 X 3 MM, RED, TAN, -PIGMENT,-SLIGHT, FOCAL GREY, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE RED FLUID -CYST,-PRESENT FILLED CYST, 5 MM IN DIAMETER DEATH COMMENT (DC) : -MAMMARY CARCINOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 4754 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 586 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92425 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 555.1 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 13:50 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA) ------------------------------------------------------------------------------------------------------------------------------------ 4755 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 587 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92426 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 377.6 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 12:42 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYE-LEFT; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-SLIGHT, MULTI- >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; FOCAL VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >MASS(ES); DORSAL; WEEK 83; 10 X 10MM; -RED FOCUS(I)/AREA(S); ALL LOBES: >UNREMARKABLE VERIFIED AT NECROPSY: OBSERVED MULTIPLE DARK RED AREAS, UP TO 5 MM IN >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK DIAMETER; COLLECTED ON ROUTINE 101; 70 X 65MM; VERIFIED AT NECROPSY: SECTIONS OBSERVED THYROID (TY) : >SKIN & PELAGE; SORE/SCAB; DORSAL; -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM VERIFIED AT NECROPSY: OBSERVED PRESENT SKIN (SK) : SKIN (SK) : -MASS(ES); DORSAL SURFACE OF RIGHT -CYST, EPIDERMAL INCLUSION,-PRESENT LUMBAR REGION: SINGLE MASS, 5 X 15 X 18 MM, FIRM, RED, TAN AND BROWN; SAME ON CUT SURFACE; OVERLYING SKIN HAS A DRY, BROWN CRUSTED AREA, 8 MM IN DIAMETER (CAP 23); REGIONAL LN (SEE LUMBAR) MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL SINGLE FIRM, LOBULATED, RED AND TAN -MINERALIZATION,-MINIMAL, MULTI-FOCAL MASS, 31 X 45 X 60 MM; SAME ON CUT -B-FIBROADENOMA,-NEOPLASM PRESENT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED 4756 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 588 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92426 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 377.6 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 12:42 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 6 X 8 MM, -HEMORRHAGE,-MODERATE, MULTI-FOCAL FIRM, RED, DARK RED AND TAN -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); RIGHT -EROSION,-MODERATELY SEVERE, FOCAL HINDFOOT: SINGLE DRY, RED, BROWN -EXOSTOSES,-MODERATELY SEVERE, MULTI- ABRADED AREA, 8 X 12 MM COLLECTED FOCAL >NOTE:>LEFT HINDFOOT SORE/SCAB NOT SEEN -INFLAMMATION, ACUTE,-MODERATELY AS NOTED IN CLINICAL SEVERE, MULTI-FOCAL OBSERVATIONS LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; RIGHT, LUMBAR: 4 X 6 X 9 MM >UNREMARKABLE (LEFT IS 2 X 2 X 4 MM) (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DRY, RED MATERIAL -BONE MARROW SMEAR TAKEN -------------------------------------------- 4757 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 589 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92426 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 377.6 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 12:42 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 4758 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 590 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92427 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/14/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 542.3 GRAMS DATE AND TIME OF NECROPSY: 06/14/99 13:47 PROSECTOR: TODD ACKER RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK KIDNEY (KD) : 55; 110 X 100MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MINIMAL, FOCAL OBSERVED LIVER (LI) : >SKIN & PELAGE; SORE/SCAB; LATERAL- -HEMORRHAGE,-MINIMAL, MULTI-FOCAL ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: -NECROSIS, HEPATOCELLULAR,-SLIGHT, OBSERVED MULTI-FOCAL >APPEARANCE; THIN; VERIFIED AT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : NECROPSY: NOT OBSERVED -MASS(ES); RIGHT AXILLARY REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 120 X 90 X 80 MM (291.6 GRAMS), RED, DARK RED, TAN, VASCULAR AND SOFT; SAME ON CUT SURFACE; PORTION COLLECTED; OVERLYING SKIN HAS A SINGLE DARK RED, TAN AND DRY CRUSTED AREA, 35 X 20 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (AXILLARY) NOT IDENTIFIED DEATH COMMENT (DC) : -MAMMARY FIBROADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 4759 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 591 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92427 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/14/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 542.3 GRAMS DATE AND TIME OF NECROPSY: 06/14/99 13:47 PROSECTOR: TODD ACKER RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 4760 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 592 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92428 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/07/99 STUDY DAY OF DEATH: 428 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 384.4 GRAMS DATE AND TIME OF NECROPSY: 06/07/99 14:15 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK LUNG (LU) : 51; 85 X 75MM; VERIFIED AT NECROPSY: -MINERALIZATION, VASCULAR,-MINIMAL, OBSERVED MULTI-FOCAL >EYES; RED DISCHARGE; EYES; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MODERATE, >SKIN & PELAGE; SORE/SCAB; VENTRAL- FOCAL CERVICAL; VERIFIED AT NECROPSY: NOT LIVER (LI) : OBSERVED -HEMORRHAGE,-MINIMAL, FOCAL >SKIN & PELAGE; RED HAIR COAT; VENTRAL- -NECROSIS, HEPATOCELLULAR,-MODERATE, ABDOMINAL-LEFT; VERIFIED AT NECROPSY: FOCAL OBSERVED MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION, -GALACTOCELE,-MODERATELY SEVERE, MULTI- EXTENDING TO MID VENTRAL CERVICAL FOCAL REGION: SINGLE MASS, 100 X 80 X 50 MM, -MINERALIZATION,-MINIMAL, MULTI-FOCAL RED, DARK RED, TAN, FIRM, AND -M-CARCINOMA,-NEOPLASM PRESENT LOBULATED; SOME AREAS EXUDE A DARK BROWN CREAMY SEMIFLUID MATERIAL; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 18) SPLEEN (SP) : SPLEEN (SP) : -DIFFUSELY LIGHT; TAN -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, DIFFUSE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: LEFT IS 9 MM IN -ANGIECTASIS,-MODERATELY SEVERE, MULTI- DIAMETER, RIGHT IS 7 MM IN DIAMETER FOCAL 4761 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 593 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92428 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/07/99 STUDY DAY OF DEATH: 428 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 384.4 GRAMS DATE AND TIME OF NECROPSY: 06/07/99 14:15 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,- MODERATELY SEVERE, FOCAL -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-MODERATELY SEVERE, MULTI- FOCAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 7 X 5 X 3 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT SALIV GL, MANDIB (SG) : SALIV GL, MANDIB (SG) : >NOTE:>DUE TO MASS, LEFT SALIVARY GLAND >NOT REQUIRED TO BE EXAMINED FOR ANIMAL IS MISSING STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK BROWN AREAS, UP TO 3 X 1 MM DEATH COMMENT (DC) : -MAMMARY CARCINOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; DARK RED, DRY MATERIAL -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY MATERIAL -------------------------------------------- 4762 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 594 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92428 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/07/99 STUDY DAY OF DEATH: 428 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 384.4 GRAMS DATE AND TIME OF NECROPSY: 06/07/99 14:15 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 4763 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 595 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92429 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/10/99 STUDY DAY OF DEATH: 431 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/10/99 14:30 PROSECTOR: DAVID SCHUETTE RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LUNG (LU) : LUNG (LU) : VERIFIED AT NECROPSY: NOT OBSERVED -UNCOLLAPSED; ALL LOBES >UNREMARKABLE -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 5 MM IN DIAMETER KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL LIVER (LI) : LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO MULTIPLE TAN, SLIGHTLY RAISED AREAS, NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT UP TO 4 MM IN DIAMETER (COLLECTED WITH ROUTINE SECTIONS) SPLEEN (SP) : SPLEEN (SP) : -LARGE; 60 X 12 X 6 MM -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT, MULTI-FOCAL CECUM (CE) : CECUM (CE) : -MASS(ES); WITHIN LUMEN, ATTACHED TO -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO MUCOSA: SINGLE MASS, 40 X 25 X 20 MM, NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT DARK RED, TAN AND SOFT; SAME ON CUT SURFACE (COLLECTED INTACT); REGIONAL LN (MESENTERIC) NORMAL DEATH COMMENT (DC) : -HISTIOCYTIC SARCOMA,-PRESENT 4764 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 596 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92429 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/10/99 STUDY DAY OF DEATH: 431 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/10/99 14:30 PROSECTOR: DAVID SCHUETTE RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; RIGHT: DRY DARK BROWN MATERIAL -DISCHARGE-NASAL; DRY BROWN MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), MAMMARY, FEMALE (MF) ------------------------------------------------------------------------------------------------------------------------------------ 4765 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 597 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92430 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 506.9 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 7:59 PROSECTOR: WENDY CHERRY RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LUNG (LU) : LEFT; VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, VASCULAR,-MINIMAL, >MASS(ES); VENTRAL-ABDOMINAL; WEEK 102; FOCAL 40 X 40MM; VERIFIED AT NECROPSY: KIDNEY (KD) : OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI- FOCAL LIVER (LI) : LIVER (LI) : -DARK FOCUS(I)/AREA(S); RIGHT MEDIAN >UNREMARKABLE LOBE: SINGLE DARK BROWN AREA, 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); MID ABDOMINAL REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL SINGLE MASS, 60 X 45 X 15 MM, GRAY, -B-FIBROADENOMA,-NEOPLASM PRESENT DARK RED AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 9 X 6 MM, DARK RED, TAN -HEMORRHAGE,-MINIMAL, MULTI-FOCAL AND SOFT -B-ADENOMA,-NEOPLASM PRESENT 4766 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 598 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92430 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 506.9 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 7:59 PROSECTOR: WENDY CHERRY RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); LEFT -CYST,-PRESENT HINDFOOT: SINGLE DARK RED AND TAN -FIBROSIS,-MODERATE, DIFFUSE ABRADED AREA, 8 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 4767 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 599 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92431 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 593.8 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:30 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK KIDNEY (KD) : 95; 50 X 50MM; VERIFIED AT NECROPSY: -CAST, PROTEINACEOUS,-MINIMAL, MULTI- OBSERVED FOCAL >MASS(ES); AXILLARY REGION-RIGHT; WEEK LIVER (LI) : 96; 40 X 30MM; VERIFIED AT NECROPSY: -FOCUS, ALTERED HEPATOCELLULAR, OBSERVED EOSINOPHILIC,-MODERATELY SEVERE, FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT SINGLE MASS, 40 X 27 X 15 MM, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 55 X 55 X 20 MM, TAN, GRAY AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 4768 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 600 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92431 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 593.8 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:30 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 5 X 4 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GRAY AND SOFT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 4769 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 601 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92432 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/24/99 STUDY DAY OF DEATH: 537 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 312.1 GRAMS DATE AND TIME OF NECROPSY: 09/24/99 12:47 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : 65; 50 X 45MM; VERIFIED AT NECROPSY: -MASS(ES); LEFT AXILLARY REGION -GALACTOCELE,-SEVERE, MULTI-FOCAL OBSERVED EXTENDING TO LATERAL THORACIC REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT >APPEARANCE; MALOCCLUSION; VERIFIED AT SINGLE MASS, 55 X 50 X 24 MM, RED, NECROPSY: OBSERVED TAN, FIRM AND LOBULATED; SOME SECTIONS >APPEARANCE; HUNCHED; VERIFIED AT EXUDE A CREAMY WHITE SEMIFLUID; NECROPSY: NOT APPLICABLE COLLECTED INTACT; REGIONAL LN >BEHAVIOR; HYPOACTIVE; VERIFIED AT (AXILLARY) NORMAL (CAP 18) NECROPSY: NOT APPLICABLE BRAIN (BR) : BRAIN (BR) : >RESPIRATION; LABORED; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 9 X 7 X 6 MM (LEFT IS 6 -ANGIECTASIS,-MODERATE, MULTI-FOCAL X 4 X 3 MM) -DEGENERATION, CYSTIC, CORTICAL,- SEVERE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -THROMBUS,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 13 X 8 X 6 MM, -HEMORRHAGE,-SEVERE, MULTI-FOCAL RED, DARK RED, TAN, SOFT AND LOBULATED -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : UTERUS (UT) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -DILATATION,-MINIMAL, DIFFUSE FILLED CYST, 5 MM IN DIAMETER 4770 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 602 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92432 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/24/99 STUDY DAY OF DEATH: 537 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 312.1 GRAMS DATE AND TIME OF NECROPSY: 09/24/99 12:47 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE WHITE MUCOID MATERIAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: RED -HEMORRHAGE,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 4771 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 603 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92433 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 362.3 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:04 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0407 .0112 % .0208 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0424 .0117 % .0216 WEIGHT TAKEN BRAIN (BR) 1.9586 .5406 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.2071 .3332 % .6163 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.1803 .3258 % .6026 WEIGHT TAKEN LIVER (LI) 10.6892 2.9504 % 5.4576 WEIGHT TAKEN LUNG (LU) 1.5529 .4286 % .7929 WEIGHT TAKEN LEFT OVARY (OV0) .0273 .0075 % .0139 WEIGHT TAKEN RIGHT OVARY (OV1) .0651 .0180 % .0332 WEIGHT TAKEN SPLEEN (SP) .5780 .1595 % .2951 WEIGHT TAKEN LF THYROID/PARA (TT0) .0184 .0051 % .0094 WEIGHT TAKEN RT THYROID/PARA (TT1) .0144 .0040 % .0074 WEIGHT TAKEN UTERUS (UT) .9246 .2552 % .4721 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS DIFFUSE: -GALACTOCELE,-MODERATELY SEVERE, MULTI- MULTIPLE TAN CREAMY SEMIFLUID FILLED FOCAL CYSTS, UP TO 10 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION 4772 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 604 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92433 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 362.3 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:04 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; -HEMORRHAGE,-MODERATELY SEVERE, MULTI- COLLECTED FOCAL GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 4773 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 605 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92434 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/29/99 11:26 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); SHOULDER-RIGHT; WEEK 55; 80 LUNG (LU) : X 65MM; VERIFIED AT NECROPSY: OBSERVED -HYPERPLASIA, TYPE II PNEUMOCYTE,- >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; MINIMAL, FOCAL WEEK 70; 40 X 50MM ANTERIOR; VERIFIED -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL AT NECROPSY: OBSERVED KIDNEY (KD) : >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; -MINERALIZATION, PELVIC,-SLIGHT, MULTI- WEEK 74; 100 X 75MM POSTERIOR; FOCAL VERIFIED AT NECROPSY: OBSERVED MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : >APPEARANCE; THIN; VERIFIED AT -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL NECROPSY: OBSERVED SINGLE MASS, 80 X 55 X 25 MM, TAN, -MINERALIZATION,-MINIMAL, MULTI-FOCAL GRAY, LOBULATED AND FIRM; SAME ON CUT -B-FIBROADENOMA,- 3 NEOPLASMS PRESENT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 50 X 40 X 35 MM, RED, DARK RED, GRAY, TAN, LOBULATED, AND FIRM; SOME SECTIONS EXUDE A TAN AND CREAMY SEMIFLUID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED 4774 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 606 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92434 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/29/99 11:26 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #3; RIGHT INGUINAL REGION, CAUDAL TO MASS #2: SINGLE MASS, 80 X 60 X 45 MM, RED, DARK RED, TAN, LOBULATED AND FIRM; SOME SECTIONS EXUDE A DARK RED SEMISOLID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 10 MM IN DIAMETER (LEFT -ANGIECTASIS,-SLIGHT, MULTI-FOCAL IS 5 MM IN DIAMETER) -DEGENERATION, CYSTIC, CORTICAL,- SEVERE, FOCAL -HEMORRHAGE,-MODERATELY SEVERE, MULTI- FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -THROMBUS,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL TAN, GRAY AND SOFT -PIGMENT,-MINIMAL, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT UTERUS (UT) : UTERUS (UT) : -CYST(S); RIGHT HORN, WITHIN WALL: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MULTIPLE CLEAR FLUID FILLED CYSTS, UP SLIGHT, MULTI-FOCAL TO 5 X 3 X 3 MM DEATH COMMENT (DC) : -PITUITARY CARCINOMA,-PRESENT 4775 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 607 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92434 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/29/99 11:26 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 4776 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 608 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92435 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 241.8 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:47 PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 10.0261 4.1464 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : UNVERIFIED AT NECROPSY -NO MACROSCOPIC LESIONS -------------------------------------------- 4777 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 609 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92435 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 241.8 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:47 PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) ------------------------------------------------------------------------------------------------------------------------------------ 4778 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 610 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92436 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/31/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 421.7 GRAMS DATE AND TIME OF NECROPSY: 01/31/00 14:02 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, FOCAL >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK -MINERALIZATION, PELVIC,-SLIGHT, MULTI- 66; 50 X 60MM; VERIFIED AT NECROPSY: FOCAL OBSERVED SKIN (SK) : SKIN (SK) : >MASS(ES); AXILLARY REGION-LEFT; WEEK >NOTE:>DUE TO LOCATION OF MASS, ROUTINE >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 74; 40 X 50MM; VERIFIED AT NECROPSY: SECTION OF SKIN/MAMMARY GLAND OBSERVED COLLECTED FROM RIGHT INGUINAL >APPEARANCE; THIN; VERIFIED AT REGION NECROPSY: OBSERVED MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -GALACTOCELE,-SEVERE, MULTI-FOCAL SINGLE MASS, 60 X 55 X 28 MM, RED, -MINERALIZATION,-MINIMAL, MULTI-FOCAL DARK RED, TAN, BROWN, LOBULATED, -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT CYSTIC, AND SOFT; ON SECTION, EXUDES A YELLOW CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 19) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION 4779 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 611 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92436 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/31/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 421.7 GRAMS DATE AND TIME OF NECROPSY: 01/31/00 14:02 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 72 X 53 X 48 MM, RED, DARK RED, TAN, BROWN, CYSTIC, LOBULATED, VASCULAR, AND SOFT; ON SECTION, EXUDES A TAN, CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED >NOTE:>DUE TO LOCATION OF MASS, ROUTINE SECTION OF SKIN/MAMMARY GLAND COLLECTED FROM RIGHT INGUINAL REGION BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 7 X 5 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN, LOBULATED AND SOFT -B-ADENOMA,-NEOPLASM PRESENT OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR -CYST,-PRESENT FLUID FILLED CYST ON EACH, LEFT IS 2 MM IN DIAMETER, RIGHT IS 5 MM IN DIAMETER CERVIX (CV) : CERVIX (CV) : -LARGE; UP TO 14 MM IN DIAMETER -DILATATION,-SEVERE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL -M-SARCOMA, ENDOMETRIAL STROMAL,- NEOPLASM PRESENT 4780 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 612 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92436 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/31/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 421.7 GRAMS DATE AND TIME OF NECROPSY: 01/31/00 14:02 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: -INFLAMMATION, ACUTE,-MODERATE, MULTI- TAN, MUCOID SEMIFLUID FOCAL -THICKENED WALL; DIFFUSE -N-SARCOMA, ENDOMETRIAL STROMAL,- NEOPLASM PRESENT DEATH COMMENT (DC) : -ENDOMETRIAL STROMAL SARCOMA,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS UP TO 7 X 5 -HEMORRHAGE,-MINIMAL, MULTI-FOCAL X 3 MM (CAP 18) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY, CRUSTED MATERIAL -ANIMAL THIN -------------------------------------------- 4781 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 613 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92436 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/31/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 421.7 GRAMS DATE AND TIME OF NECROPSY: 01/31/00 14:02 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 4782 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 614 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92437 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/19/99 STUDY DAY OF DEATH: 348 STUDY WEEK OF DEATH: 50 TERMINAL BODY WEIGHT: 262.3 GRAMS DATE AND TIME OF NECROPSY: 03/19/99 7:52 PROSECTOR: GREGORY CODDINGTON RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : 34; 30 X 20MM; VERIFIED AT NECROPSY: -MASS(ES); LEFT INGUINAL REGION: -GALACTOCELE,-MODERATELY SEVERE, MULTI- OBSERVED SINGLE MASS, 30 X 22 X 14 MM, RED, FOCAL >EYES; RED DISCHARGE; EYES; VERIFIED AT DARK RED, TAN, GRAY, LOBULATED AND -MINERALIZATION,-SLIGHT, FOCAL NECROPSY: OBSERVED FIRM; SAME ON CUT SURFACE (CAP 19); -M-CARCINOMA,-NEOPLASM PRESENT >APPEARANCE; THIN; VERIFIED AT REGIONAL LN (INGUINAL) LEFT IS NORMAL, NECROPSY: OBSERVED RIGHT NOT IDENTIFIED (CAP 20) >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -CYST(S); BILATERAL AXILLARY REGIONS: AT NECROPSY: NOT OBSERVED MULTIPLE TAN AND CREAMY SEMIFLUID >BEHAVIOR; ATAXIC; VERIFIED AT FILLED CYSTS, UP TO 8 X 7 X 7 MM (CAP NECROPSY: NOT APPLICABLE 21) BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATE, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, RED, DARK -HEMORRHAGE,-SEVERE, MULTI-FOCAL RED, GRAY AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE -EDEMA,-MINIMAL, FOCAL VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE MODERATE AMOUNT OF A LIGHT GREEN AND MUCOID SEMIFLUID MATERIAL 4783 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 615 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92437 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/19/99 STUDY DAY OF DEATH: 348 STUDY WEEK OF DEATH: 50 TERMINAL BODY WEIGHT: 262.3 GRAMS DATE AND TIME OF NECROPSY: 03/19/99 7:52 PROSECTOR: GREGORY CODDINGTON RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; LEFT: 15 X 12 X 10 MM (FOR >UNREMARKABLE COMPARISON, RIGHT IS 6 X 1 X 1 MM) (CAP 18) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY MATERIAL -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), LN, MANDIBULAR (MN), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), LN, INGUINAL (IN) ------------------------------------------------------------------------------------------------------------------------------------ 4784 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 616 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92438 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 348.6 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:15 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0325 .0093 % .0174 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0300 .0086 % .0161 WEIGHT TAKEN BRAIN (BR) 1.8684 .5360 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.1359 .3258 % .6080 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.1676 .3349 % .6249 WEIGHT TAKEN LIVER (LI) 9.3979 2.6959 % 5.0299 WEIGHT TAKEN LUNG (LU) 1.3652 .3916 % .7307 WEIGHT TAKEN LEFT OVARY (OV0) .0463 .0133 % .0248 WEIGHT TAKEN RIGHT OVARY (OV1) .0548 .0157 % .0293 WEIGHT TAKEN SPLEEN (SP) .6518 .1870 % .3489 WEIGHT TAKEN LF THYROID/PARA (TT0) .0119 .0034 % .0064 WEIGHT TAKEN RT THYROID/PARA (TT1) .0118 .0034 % .0063 WEIGHT TAKEN UTERUS (UT) 1.0419 .2989 % .5576 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL; WEEK 44; KIDNEY (KD) : 10 X 10MM; VERIFIED AT NECROPSY: -CAST, PROTEINACEOUS,-MINIMAL, MULTI- OBSERVED FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT 4785 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 617 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92438 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 348.6 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:15 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); BILATERAL INGUINAL REGIONS: -GALACTOCELE,-MODERATELY SEVERE, MULTI- MULTIPLE TAN CREAMY SEMIFLUID FILLED FOCAL CYSTS, UP TO 12 X 8 X 7 MM (CAP 18); POSSIBLY CONSISTENT WITH MASS NOTED IN CLINICAL OBSERVATIONS DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU) ------------------------------------------------------------------------------------------------------------------------------------ 4786 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 618 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92439 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 438.6 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:04 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CYST,-SLIGHT, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-SLIGHT, FOCAL LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -FOCUS, ALTERED HEPATOCELLULAR, MULTIPLE DARK RED AREAS, UP TO 5 MM IN EOSINOPHILIC,-SLIGHT, FOCAL DIAMETER, SUNKEN WITH A STELLATE -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- PATTERN; COLLECTED ON ROUTINE SECTIONS FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 12 MM IN DIAMETER DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4787 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 619 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92439 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 438.6 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:04 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), MAMMARY, FEMALE (MF), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 4788 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 620 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92440 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 560 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/17/99 9:41 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); HEAD-MAXILLARY-RIGHT; WEEK LUNG (LU) : LUNG (LU) : 66; 10 X 10MM; VERIFIED AT NECROPSY: -UNCOLLAPSED; ALL LOBES >UNREMARKABLE OBSERVED KIDNEY (KD) : >SKIN & PELAGE; SORE/SCAB; HEAD- -MINERALIZATION, PELVIC,-MINIMAL, MULTI- MAXILLARY-RIGHT; VERIFIED AT NECROPSY: FOCAL OBSERVED -PYELONEPHRITIS,-SLIGHT, FOCAL >APPEARANCE; LIMITED USE; LIMB-HIND- SKIN (SK) : SKIN (SK) : LEFT; VERIFIED AT NECROPSY: NOT -MASS(ES); ATTACHED TO RIGHT VENTRAL -B-BASAL CELL TUMOR,-NEOPLASM PRESENT APPLICABLE CERVICAL REGION: SINGLE MASS, 11 X 10 X 5 MM, RED, DARK RED, GREY AND FIRM; SAME ON CUT SURFACE (CAP 18); REGIONAL LN (MANDIBULAR) NORMAL (CAP 19); OVERLYING SKIN HAS A SINGLE DRY, DARK RED CRUSTED AREA, 4 MM IN DIAMETER; COLLECTED WITH MASS BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 5 MM IN DIAMETER (RIGHT -ANGIECTASIS,-MINIMAL, MULTI-FOCAL IS 3 MM IN DIAMETER) -DEGENERATION, CYSTIC, CORTICAL,- MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -THROMBUS,-MODERATELY SEVERE, FOCAL 4789 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 621 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92440 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 560 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/17/99 9:41 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 10 X 9 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT LOBULATED AND SOFT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -LARGE; LEFT HINDFOOT: 44 X 18 X 18 XX -EXOSTOSES,-SLIGHT, MULTI-FOCAL (RIGHT IS 44 X 10 X 9 MM); LEFT -FIBROSIS,-SEVERE, MULTI-FOCAL APPEARS SWOLLEN; BOTH COLLECTED -INFLAMMATION, CHRONIC ACTIVE,- MODERATE, MULTI-FOCAL GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -------------------------------------------- 4790 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 622 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92440 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 560 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/17/99 9:41 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY), MAMMARY, FEMALE (MF), ADRENAL, MEDULLA (MA), LN, MANDIBULAR (MN) ------------------------------------------------------------------------------------------------------------------------------------ 4791 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 623 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92441 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 450.3 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 9:46 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0457 .0101 % .0218 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0428 .0095 % .0204 WEIGHT TAKEN BRAIN (BR) 2.0955 .4654 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.6868 .3746 % .8050 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.6612 .3689 % .7927 WEIGHT TAKEN LIVER (LI) 12.7468 2.8307 % 6.0829 WEIGHT TAKEN LUNG (LU) 1.6925 .3759 % .8077 WEIGHT TAKEN LEFT OVARY (OV0) .0693 .0154 % .0331 WEIGHT TAKEN RIGHT OVARY (OV1) .0623 .0138 % .0297 WEIGHT TAKEN SPLEEN (SP) .8104 .1800 % .3867 WEIGHT TAKEN LF THYROID/PARA (TT0) .0121 .0027 % .0058 WEIGHT TAKEN RT THYROID/PARA (TT1) .0106 .0024 % .0051 WEIGHT TAKEN UTERUS (UT) 1.1020 .2447 % .5259 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; DORSAL; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-SLIGHT, FOCAL >MASS(ES); INGUINAL REGION-LEFT; WEEK SKIN (SK) : SKIN (SK) : 48; 20 X 15MM; VERIFIED AT NECROPSY: -CRUSTED AREA(S); RIGHT FLANK: SINGLE -EROSION,-MODERATE, FOCAL OBSERVED TAN, DRY AREA, 8 MM IN DIAMETER (CAP -INFLAMMATION, ACUTE,-MINIMAL, FOCAL 19) MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -FIBROSIS,-MODERATELY SEVERE, FOCAL SINGLE MASS, 13 MM IN DIAMETER, TAN, -GALACTOCELE,-SEVERE, MULTI-FOCAL BROWN, RED AND SOFT; ON SECTION EXUDES -HEMORRHAGE,-MODERATE, FOCAL A DARK RED FLUID (CAP 18); REGIONAL LN (INGUINAL) NOT IDENTIFIED 4792 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 624 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92441 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 450.3 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 9:46 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM -B-ADENOMA,-NEOPLASM PRESENT -RED FOCUS(I)/AREA(S); LEFT AND RIGHT ASPECT: SINGLE DARK RED AREA ON EACH, EACH IS 3 MM IN DIAMETER DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 4793 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 625 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92441 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 450.3 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 9:46 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 4794 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 626 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92442 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/25/99 STUDY DAY OF DEATH: 476 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 386.7 GRAMS DATE AND TIME OF NECROPSY: 07/25/99 10:27 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NO FECES; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >SKIN & PELAGE; RED HAIR COAT; HEAD- FOCAL ENTIRE; VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, FOCAL >SKIN & PELAGE; YELLOW HAIR COAT; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC -VACUOLATION, HEPATOCELLULAR,- >DISCHARGE; NASAL; RED IN COLOR; SURFACE OF RIGHT MEDIAN LOBE: SINGLE MODERATELY SEVERE, MULTI-FOCAL VERIFIED AT NECROPSY: OBSERVED RED AREA, 3 X 2 MM; COLLECTED ON >BEHAVIOR; HYPOACTIVE; MILD; VERIFIED ROUTINE SECTION AT NECROPSY: NOT APPLICABLE THYROID (TY) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT NECROPSY: OBSERVED BRAIN (BR) : BRAIN (BR) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 9 X 9 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, TAN AND SOFT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -EROSION,-SLIGHT, FOCAL TAN ERODED AREAS, UP TO 3 X 2 MM -INFLAMMATION, ACUTE,-MINIMAL, FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 4795 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 627 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92442 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/25/99 STUDY DAY OF DEATH: 476 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 386.7 GRAMS DATE AND TIME OF NECROPSY: 07/25/99 10:27 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: DRY, YELLOW MATERIAL; POSSIBLY CONSISTENT WITH STAINS (HAIRCOAT-YELLOW) AS NOTED IN CLINICAL OBSERVATIONS -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL; POSSIBLY CONSISTENT WITH STAINS (HEAD-RED) AS NOTED IN CLINICAL OBSERVATIONS -DISCHARGE-NASAL; DRY, RED MATERIAL -------------------------------------------- 4796 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 628 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92442 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/25/99 STUDY DAY OF DEATH: 476 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 386.7 GRAMS DATE AND TIME OF NECROPSY: 07/25/99 10:27 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), MAMMARY, FEMALE (MF) ------------------------------------------------------------------------------------------------------------------------------------ 4797 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 629 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92443 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 593.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:15 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- MULTIPLE DARK RED AREAS, UP TO 7 X 9 FOCAL MM; COLLECTED ON ROUTINE SECTIONS PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 X 6 X 7 MM (RIGHT IS 3 -ANGIECTASIS,-MODERATELY SEVERE, MULTI- X 4 X 5 MM) FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, FOCAL -THROMBUS,-MINIMAL, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 7 X 10 MM, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL FIRM, RED, DARK RED AND TAN -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4798 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 630 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92443 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 593.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:15 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 4799 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 631 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92444 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 424.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:33 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK KIDNEY (KD) : 80; 20 X 20MM; VERIFIED AT NECROPSY: -CAST, PROTEINACEOUS,-MINIMAL, MULTI- OBSERVED FOCAL >MASS(ES); INGUINAL REGION-RIGHT; WEEK -DILATATION, PELVIC,-PRESENT 92; 35 X 35MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-SLIGHT, MULTI- OBSERVED FOCAL >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; LIVER (LI) : WEEK 101; 55 X 50MM; VERIFIED AT -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- NECROPSY: OBSERVED FOCAL >SKIN & PELAGE; SORE/SCAB; PERINEAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- AREA; VERIFIED AT NECROPSY: NOT FOCAL OBSERVED MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL SINGLE MASS, 55 X 40 X 21 MM, RED, -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT TAN, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 30 X 20 X 15 MM, DARK RED, TAN, AND LOBULATED; SOME SECTIONS EXUDE A DARK BROWN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED MUSCLE, SKELETAL (SM) : -I-LEIOMYOSARCOMA,-NEOPLASM PRESENT 4800 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 632 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92444 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 424.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:33 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MUSCLE, SKELETAL (SM) : -MASS(ES); SURROUNDS RECTUM AND BASE OF TAIL: SINGLE MASS, 50 X 45 X 25 MM, TAN, DARK RED, LOBULATED AND FIRM; SAME ON CUT SURFACE; POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL RIGHT) AS NOTED IN CLINICAL OBSERVATIONS; PORTION COLLECTED WITH RECTUM; REMAINING PORTION COLLECTED WITH TAIL; REGIONAL LN (LUMBAR) (CAP 22) ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 4 X 3 MM -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -M-LEIOMYOSARCOMA,-NEOPLASM PRESENT OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID >UNREMARKABLE FILLED CYST, 4 MM IN DIAMETER DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4801 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 633 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92444 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 424.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:33 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 4802 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 634 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92445 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/31/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 795.2 GRAMS DATE AND TIME OF NECROPSY: 01/31/00 14:50 PROSECTOR: STEVE VAN ADESTINE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; OBESE; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >EXCRETION; FEW FECES; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MINIMAL, MULTI- >SKIN & PELAGE; BROWN HAIR COAT; FOCAL PERINEAL AREA; VERIFIED AT NECROPSY: LIVER (LI) : LIVER (LI) : OBSERVED -MOTTLED; ALL LOBES: RED AND TAN -VACUOLATION, HEPATOCELLULAR,- >DISCHARGE; NASAL; RED IN COLOR; MODERATELY SEVERE, MULTI-FOCAL VERIFIED AT NECROPSY: OBSERVED MAMMARY, FEMALE (MF) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -GALACTOCELE,-SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 7 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL TAN, GRAY, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE LIGHT GREEN MUCOID SEMIFLUID DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; YELLOW MOIST MATERIAL 4803 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 635 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92445 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/31/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 795.2 GRAMS DATE AND TIME OF NECROPSY: 01/31/00 14:50 PROSECTOR: STEVE VAN ADESTINE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -ANIMAL OBESE -DISCHARGE-NASAL; DARK RED DRY CRUSTY MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 4804 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 636 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92446 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/05/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 328.5 GRAMS DATE AND TIME OF NECROPSY: 11/05/99 13:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYES; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL, FOCAL >DISCHARGE; NASAL; RED IN COLOR; -PYELONEPHRITIS,-SLIGHT, FOCAL VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : >SKIN & PELAGE; BROWN HAIR COAT; -VACUOLATION, HEPATOCELLULAR,- VENTRAL-ABDOMINAL; VERIFIED AT MODERATELY SEVERE, MULTI-FOCAL NECROPSY: OBSERVED MAMMARY, FEMALE (MF) : >EXCRETION; NO FECES; VERIFIED AT -GALACTOCELE,-SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE BRAIN (BR) : BRAIN (BR) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE PITUITARY (PI) : PITUITARY (PI) : >APPEARANCE; HEAD TILT; RIGHT; VERIFIED -MASS(ES); 10 X 10 X 8 MM, RED, GREY, -B-ADENOMA,-NEOPLASM PRESENT AT NECROPSY: OBSERVED TAN AND SOFT >SKIN & PELAGE; BROWN HAIR COAT; STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : PERINEAL AREA; VERIFIED AT NECROPSY: -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS,-MODERATE, DIFFUSE OBSERVED TAN AND SLIGHTLY ERODED AREAS, UP TO 2 -EDEMA,-MODERATE, FOCAL >BEHAVIOR; ATAXIC; VERIFIED AT X 1 MM -EROSION,-MINIMAL, FOCAL NECROPSY: NOT APPLICABLE -HYPERKERATOSIS, NONGLANDULAR MUCOSA,- >APPEARANCE; THIN; VERIFIED AT MODERATE, DIFFUSE NECROPSY: OBSERVED -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 4805 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 637 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92446 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/05/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 328.5 GRAMS DATE AND TIME OF NECROPSY: 11/05/99 13:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS, EXTENDING TO VENTRAL ABDOMINAL REGION: MOIST, BROWN MATERIAL -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -DISCHARGE-NASAL; DRY, RED MATERIAL -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 4806 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 638 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92447 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 294.4 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 8:27 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT LUNG (LU) : NECROPSY: OBSERVED -MINERALIZATION, VASCULAR,-MINIMAL, >EXCRETION; FEW FECES; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : KIDNEY (KD) : >BEHAVIOR; ATAXIC; VERIFIED AT -GRANULAR MATERIAL; BILATERAL, WITHIN -CAST, PROTEINACEOUS,-MINIMAL, MULTI- NECROPSY: NOT APPLICABLE PELVIS: MULTIPLE TAN AND FIRM FOCAL >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; GRANULES, UP TO 1 MM IN DIAMETER -MINERALIZATION, PELVIC,-SLIGHT, MULTI- VERIFIED AT NECROPSY: NOT APPLICABLE -DIFFUSELY LIGHT; BILATERAL: TAN FOCAL LIVER (LI) : LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN >UNREMARKABLE MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 10 X 8 MM, RED, TAN, -B-ADENOMA,-NEOPLASM PRESENT AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE BROWN FOCI, UP TO 1 MM IN DIAMETER DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4807 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 639 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92447 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 294.4 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 8:27 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 4808 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 640 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92448 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 362.2 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 14:43 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HEAD TILT; RIGHT; VERIFIED LUNG (LU) : AT NECROPSY: NOT APPLICABLE -MINERALIZATION, VASCULAR,-MINIMAL, >APPEARANCE; MALOCCLUSION; VERIFIED AT FOCAL NECROPSY: OBSERVED KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT THYMUS (TH) : THYMUS (TH) : -LARGE; 6 X 12 X 13 MM -M-FIBROSARCOMA,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 7 X 12 MM, -CYST,-PRESENT FIRM, RED, DARK RED, TAN AND BROWN -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -------------------------------------------- 4809 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 641 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92448 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 362.2 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 14:43 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY), MAMMARY, FEMALE (MF) ------------------------------------------------------------------------------------------------------------------------------------ 4810 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 642 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92449 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 356.6 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:46 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) .0453 .0127 % .0223 WEIGHT TAKEN RIGHT ADRENAL (AD1) .0417 .0117 % .0205 WEIGHT TAKEN BRAIN (BR) 2.0299 .5692 % 1.0000 WEIGHT TAKEN LEFT KIDNEY (KD0) 1.1844 .3321 % .5835 WEIGHT TAKEN RIGHT KIDNEY (KD1) 1.1588 .3250 % .5709 WEIGHT TAKEN LIVER (LI) 10.6589 2.9890 % 5.2509 WEIGHT TAKEN LUNG (LU) 1.4643 .4106 % .7214 WEIGHT TAKEN LEFT OVARY (OV0) .0787 .0221 % .0388 WEIGHT TAKEN RIGHT OVARY (OV1) .0768 .0215 % .0378 WEIGHT TAKEN SPLEEN (SP) .7269 .2038 % .3581 WEIGHT TAKEN LF THYROID/PARA (TT0) .0205 .0057 % .0101 WEIGHT TAKEN RT THYROID/PARA (TT1) .0152 .0043 % .0075 WEIGHT TAKEN UTERUS (UT) .8613 .2415 % .4243 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : VERIFIED AT NECROPSY: OBSERVED -CYST(S); ALL REGIONS DIFFUSE: -GALACTOCELE,-SLIGHT, MULTI-FOCAL MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT -DIFFUSELY RED; DARK RED DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN 4811 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 643 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92449 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 356.6 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:46 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 4812 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 644 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92450 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 619 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 272.2 GRAMS DATE AND TIME OF NECROPSY: 12/15/99 14:08 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL; WEEK 84; KIDNEY (KD) : 30 X 30MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MODERATE, OBSERVED MULTI-FOCAL >MASS(ES); VENTRAL-ABDOMINAL; WEEK 75; LIVER (LI) : LIVER (LI) : 20 X 20MM; VERIFIED AT NECROPSY: -CYST(S); LEFT LATERAL LOBE: MULTIPLE >UNREMARKABLE OBSERVED CLEAR FLUID FILLED CYSTS, UP TO 1 MM >SKIN & PELAGE; SORE/SCAB; VENTRAL- IN DIAMETER; COLLECTED ON ROUTINE CERVICAL; VERIFIED AT NECROPSY: NOT SECTION OBSERVED PANCREAS (PA) : >EXCRETION; FEW FECES; VERIFIED AT -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL NECROPSY: NOT APPLICABLE MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : >APPEARANCE; THIN; VERIFIED AT -MASS(ES); MID CERVICAL REGION: SINGLE -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT NECROPSY: OBSERVED MASS, 40 X 30 X 15 MM, YELLOW, TAN, -M-CARCINOMA,-NEOPLASM PRESENT >APPEARANCE; HEAD TILT; LEFT; VERIFIED LOBULATED, AND SOFT; CUT SURFACE AT NECROPSY: NOT APPLICABLE CONTAINS A CREAMY YELLOW SEMISOLID; >BEHAVIOR; ATAXIC; VERIFIED AT COLLECTED; REGIONAL LN (MANDIBULAR) NECROPSY: NOT APPLICABLE NORMAL; COLLECTED -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 40 X 25 X 15 MM, TAN, DARK RED, AND SOFT; CUT SURFACE CONTAINS A TAN CREAMY SEMISOLID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED 4813 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 645 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92450 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 619 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 272.2 GRAMS DATE AND TIME OF NECROPSY: 12/15/99 14:08 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #3; LEFT INGUINAL REGION: SINGLE MASS, 30 X 25 X 25 MM, RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 10 X 8 MM, DARK RED, -HEMORRHAGE,-SEVERE, MULTI-FOCAL RED, GRAY, AND SOFT -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MULTIPLE CLEAR FLUID FILLED CYSTS, UP MODERATE, FOCAL TO 8 MM IN DIAMETER -SCLEROSIS,-MODERATELY SEVERE, MULTI- -THICKENED WALL; BILATERAL, DIFFUSE FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID >UNREMARKABLE FILLED CYST, 3 MM IN DIAMETER DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 4814 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 646 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92450 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 619 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 272.2 GRAMS DATE AND TIME OF NECROPSY: 12/15/99 14:08 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), LN, MANDIBULAR (MN) ------------------------------------------------------------------------------------------------------------------------------------ 4815 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 647 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92451 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 517.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:06 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; PAWS-HIND; LUNG (LU) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI- FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI- FOCAL LIVER (LI) : LIVER (LI) : -DARK FOCUS(I)/AREA(S); RIGHT MEDIAN -FOCUS, ALTERED HEPATOCELLULAR, LOBE: TWO BROWN AREAS, EACH IS 3 MM IN EOSINOPHILIC,-MODERATELY SEVERE, FOCAL DIAMETER; COLLECTED ON ROUTINE SECTIONS MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT SPLEEN (SP) : SPLEEN (SP) : -LARGE; 50 X 16 X 10 MM -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 10 X 7 X 7 MM (LEFT IS -ANGIECTASIS,-MODERATE, MULTI-FOCAL 6 X 4 X 4 MM) -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL 4816 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 648 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92451 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 517.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:06 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -NECROSIS, MEDULLARY,-SLIGHT, MULTI- FOCAL -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 4 MM, DARK RED, TAN -HEMORRHAGE,-MODERATE, MULTI-FOCAL AND SOFT -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -DILATATION,-SEVERE, DIFFUSE SINGLE CLEAR FLUID FILLED CYST ON EACH, LEFT IS 10 X 7 X 7 MM, RIGHT IS 15 X 10 X 10 MM DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; BILATERAL, TARSAL JOINT: EACH -EXOSTOSES,-MODERATELY SEVERE, MULTI- IS UP TO 18 MM IN DIAMETER; COLLECTED FOCAL -FIBROSIS,-MODERATELY SEVERE, MULTI- FOCAL -INFLAMMATION, CHRONIC, SYNOVIAL,- SLIGHT, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4817 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 649 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92451 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 517.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:06 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 4818 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 650 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92452 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 477.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:28 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LUNG (LU) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION,-MINIMAL, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI- FOCAL LIVER (LI) : LIVER (LI) : -DARK FOCUS(I)/AREA(S); LEFT MEDIAN -TELANGIECTASIS,-MINIMAL, FOCAL LOBE: SINGLE DARK BROWN AREA, 3 MM IN DIAMETER (CAP 22) BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 6 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL TAN, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -EDEMA,-MINIMAL, MULTI-FOCAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID >UNREMARKABLE FILLED CYST, 2 MM IN DIAMETER CERVIX (CV) : CERVIX (CV) : -LARGE; 10 X 10 X 8 MM -HYPERTROPHY, MUSCULAR,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : -EROSION,-MODERATE, FOCAL 4819 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 651 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92452 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 477.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:28 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); RIGHT -INFLAMMATION, CHRONIC ACTIVE,- HINDFOOT: SINGLE DARK RED ABRADED MODERATE, MULTI-FOCAL AREA, 10 X 6 X 5 MM; COLLECTED -PIGMENT,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), MAMMARY, FEMALE (MF), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 4820 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 652 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92453 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 396.5 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 13:59 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL; WEEK 80; KIDNEY (KD) : 30 X 30MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-SLIGHT, MULTI- OBSERVED FOCAL >SKIN & PELAGE; RED HAIR COAT; HEAD- MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : ENTIRE; VERIFIED AT NECROPSY: NOT -MASS(ES); RIGHT CERVICAL REGION, -GALACTOCELE,-MODERATE, MULTI-FOCAL OBSERVED EXTENDING TO RIGHT AXILLARY REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT >SKIN & PELAGE; RED HAIR COAT; VENTRAL- SINGLE MASS, 38 X 32 X 20 MM, FIRM, ABDOMINAL; VERIFIED AT NECROPSY: NOT LOBULATED, RED, AND TAN; SAME ON CUT OBSERVED SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 18) BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 14 X 9 X 6 MM, FIRM, -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LOBULATED, RED, DARK RED, AND TAN -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 4821 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 653 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92453 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 396.5 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 13:59 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 4822 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 654 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92454 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 566 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 310.8 GRAMS DATE AND TIME OF NECROPSY: 10/23/99 11:03 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL; WEEK 54; KIDNEY (KD) : 40 X 40MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MODERATE, OBSERVED MULTI-FOCAL >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : WEEK 61; 25 X 25MM; VERIFIED AT -MASS(ES); RIGHT INGUINAL REGION: -GALACTOCELE,-MODERATELY SEVERE, MULTI- NECROPSY: OBSERVED SINGLE MASS, 25 X 20 X 10 MM, TAN AND FOCAL >SKIN & PELAGE; SORE/SCAB; VENTRAL- FIRM; SAME ON CUT SURFACE; OVERLYING -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT ABDOMINAL; VERIFIED AT NECROPSY: SKIN HAS A SINGLE DARK RED, DRY, OBSERVED CRUSTED AREA, 3 X 2 MM; MASS AND >EXCRETION; FEW FECES; VERIFIED AT OVERLYING SKIN COLLECTED; REGIONAL LN NECROPSY: NOT APPLICABLE (INGUINAL) NOT IDENTIFIED >BEHAVIOR; ATAXIC; VERIFIED AT -MASS #2; RIGHT VENTRAL CERVICAL NECROPSY: NOT APPLICABLE REGION: SINGLE MASS, 45 X 30 X 25 MM, >EYES; SQUINTED; PERI-ORBITAL; VERIFIED RED, TAN, FIRM, AND LOBULATED; SAME ON AT NECROPSY: NOT APPLICABLE CUT SURFACE; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 18) BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 10 X 10 MM, RED, TAN, -HEMORRHAGE,-SLIGHT, FOCAL GRAY, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 4823 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 655 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92454 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 566 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 310.8 GRAMS DATE AND TIME OF NECROPSY: 10/23/99 11:03 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), LN, MANDIBULAR (MN) ------------------------------------------------------------------------------------------------------------------------------------ 4824 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 656 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92455 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/23/00 STUDY DAY OF DEATH: 718 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 430.3 GRAMS DATE AND TIME OF NECROPSY: 03/23/00 10:53 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; HEAD- KIDNEY (KD) : ENTIRE; VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MODERATE, >MASS(ES); AXILLARY REGION-LEFT; WEEK MULTI-FOCAL 101; 45 X 40MM; VERIFIED AT NECROPSY: LIVER (LI) : NOT OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI- >EXCRETION; FEW FECES; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI- >SKIN & PELAGE; RED HAIR COAT; SHOULDER- FOCAL LEFT; VERIFIED AT NECROPSY: NOT THYROID (TY) : THYROID (TY) : OBSERVED -DIFFUSELY RED; LEFT: DARK RED -HEMORRHAGE,-SLIGHT, MULTI-FOCAL >APPEARANCE; HEAD TILT; LEFT; VERIFIED -B-ADENOMA, C-CELL,- 2 NEOPLASMS AT NECROPSY: OBSERVED PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : NECROPSY: NOT APPLICABLE -CYST(S); ALL REGIONS, DIFFUSE: -GALACTOCELE,-MODERATELY SEVERE, MULTI- MULTIPLE TAN CREAMY SEMIFLUID FILLED FOCAL CYSTS, UP TO 40 X 25 X 19 MM (CAP 21); -MINERALIZATION,-MINIMAL, FOCAL POSSIBLY CONSISTENT WITH (MASS -B-FIBROADENOMA,-NEOPLASM PRESENT AXILLARY) NOTED IN CLINICAL OBSERVATIONS BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 6 X 5 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, SOFT AND LOBULATED 4825 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 657 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92455 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/23/00 STUDY DAY OF DEATH: 718 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 430.3 GRAMS DATE AND TIME OF NECROPSY: 03/23/00 10:53 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 3 MM IN DIAMETER DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DARK RED, DRY MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA) ------------------------------------------------------------------------------------------------------------------------------------ 4826 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 658 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92456 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 523.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:34 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK KIDNEY (KD) : 88; 45 X 40MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MODERATE, OBSERVED MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL, MULTI-FOCAL LIVER (LI) : LIVER (LI) : -RAISED FOCUS(I)/AREA(S); ALONG MARGIN -FOCUS, ALTERED HEPATOCELLULAR, OF LEFT LATERAL LOBE: SINGLE TAN BASOPHILIC,-MODERATE, FOCAL RAISED AREA, 4 X 3 X 1 MM (CAP 23) -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MINIMAL, FOCAL -THROMBUS,-MODERATELY SEVERE, FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 40 X 40 X 22 MM, RED, TAN, YELLOW, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 4827 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 659 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92456 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 523.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:34 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 4828 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 660 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92457 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 333.2 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:28 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; BROWN HAIR COAT; KIDNEY (KD) : PERINEAL AREA; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MODERATE, OBSERVED MULTI-FOCAL >APPEARANCE; HEAD TILT; RIGHT; VERIFIED MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : AT NECROPSY: NOT APPLICABLE -MASS(ES); LEFT AXILLARY REGION: -GALACTOCELE,-MODERATE, MULTI-FOCAL >APPEARANCE; THIN; VERIFIED AT SINGLE MASS, 12 X 9 X 8 MM, TAN AND -MINERALIZATION,-MINIMAL, MULTI-FOCAL NECROPSY: NOT OBSERVED FIRM; SAME ON CUT SURFACE (CAP 18); -B-FIBROADENOMA,-NEOPLASM PRESENT >MASS(ES); AXILLARY REGION-LEFT; WEEK REGIONAL LN (AXILLARY) NORMAL (CAP 19) 84; VERIFIED AT NECROPSY: OBSERVED -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE WHITE FLUID FILLED CYSTS, UP TO 3 X 2 X 2 MM; COLLECTED ON ROUTINE SECTION -THICKENED; ALL REGIONS, DIFFUSE: COLLECTED ON ROUTINE SECTION BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 MM IN DIAMETER (RIGHT -DEGENERATION, CYSTIC, CORTICAL,- IS 4 MM IN DIAMETER) MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 7 MM, DARK RED, -HEMORRHAGE,-MODERATELY SEVERE, MULTI- RED, GRAY, TAN AND SOFT FOCAL -B-ADENOMA,-NEOPLASM PRESENT 4829 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 661 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92457 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 333.2 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:28 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN LUMEN: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MULTIPLE CLEAR FLUID FILLED CYSTS, UP MODERATE, MULTI-FOCAL TO 4 MM IN DIAMETER DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; DRY, BROWN MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), ADRENAL, MEDULLA (MA), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 4830 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 662 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92458 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 470 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 254.2 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 8:37 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYES; VERIFIED AT LUNG (LU) : NECROPSY: OBSERVED -INFILTRATE, MACROPHAGE, ALVEOLAR,- >SKIN & PELAGE; BROWN HAIR COAT; MINIMAL, FOCAL PERINEAL AREA; VERIFIED AT NECROPSY: KIDNEY (KD) : NOT OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >EXCRETION; FEW FECES; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-SLIGHT, MULTI- >APPEARANCE; HUNCHED; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC -VACUOLATION, HEPATOCELLULAR,-SLIGHT, NECROPSY: OBSERVED SURFACE OF RIGHT MEDIAN LOBE: SINGLE MULTI-FOCAL >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED TAN AREA, 4 MM IN DIAMETER; COLLECTED AT NECROPSY: NOT APPLICABLE ON ROUTINE SECTION >BEHAVIOR; HYPOACTIVE; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 9 X 5 MM, RED, TAN, -CYST,-PRESENT LOBULATED, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY MATERIAL 4831 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 663 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92458 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 470 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 254.2 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 8:37 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), MAMMARY, FEMALE (MF) ------------------------------------------------------------------------------------------------------------------------------------ 4832 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 664 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92459 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 292.3 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 13:28 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK LUNG (LU) : 70; 35 X 35MM; VERIFIED AT NECROPSY: -INFILTRATE, MACROPHAGE, PIGMENTED,- OBSERVED MINIMAL, FOCAL >MASS(ES); INGUINAL REGION-RIGHT; WEEK KIDNEY (KD) : 92; 30 X 30MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MINIMAL, MULTI- OBSERVED FOCAL THYROID (TY) : -HYPERPLASIA, FOLLICULAR CELL,- MODERATE, FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -GALACTOCELE,-MODERATELY SEVERE, MULTI- SINGLE MASS, 35 X 35 X 25 MM, RED, FOCAL TAN, FIRM, AND LOBULATED; SAME ON CUT -HEMORRHAGE,-MODERATE, MULTI-FOCAL SURFACE; COLLECTED; REGIONAL LN (SEE -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT AXILLARY) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 55 X 34 X 22 MM, RED, DARK RED, TAN, BROWN, SOFT, AND LOBULATED; ON SECTION SOME AREAS CONTAIN A BROWN FLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT 4833 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 665 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92459 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 292.3 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 13:28 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 6 MM, RED, DARK -HEMORRHAGE,-MODERATE, MULTI-FOCAL RED, TAN, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -DIFFUSELY RED; RIGHT: (CAP 19) -HEMORRHAGE,-MINIMAL, MULTI-FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA) ------------------------------------------------------------------------------------------------------------------------------------ 4834 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 666 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92460 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/13/00 STUDY DAY OF DEATH: 679 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 289.2 GRAMS DATE AND TIME OF NECROPSY: 02/13/00 8:33 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL; WEEK 62; THYROID (TY) : 40 X 35MM; VERIFIED AT NECROPSY: -CYST, ULTIMOBRANCHIAL,-PRESENT OBSERVED MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : >EYES; SQUINTED; PERI-ORBITAL-LEFT; -MASS(ES); LEFT PERINEAL REGION: -GALACTOCELE,-SLIGHT, MULTI-FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE SINGLE MASS, 37 X 25 X 15 MM, TAN, -MINERALIZATION,-MINIMAL, MULTI-FOCAL >SKIN & PELAGE; SORE/SCAB; PAW-HIND- VASCULAR, LOBULATED AND SOFT; ON -B-FIBROADENOMA,-NEOPLASM PRESENT RIGHT; VERIFIED AT NECROPSY: OBSERVED SECTION EXUDES A TAN FLUID (CAP 21); -M-CARCINOMA,-NEOPLASM PRESENT >APPEARANCE; THIN; VERIFIED AT REGIONAL LN (LUMBAR) NORMAL (CAP 20) NECROPSY: OBSERVED -THICKENED; VENTRAL CERVICAL REGION: >BEHAVIOR; HYPOACTIVE; VERIFIED AT TAN AND FIRM (CAP 19) NECROPSY: NOT APPLICABLE BRAIN (BR) : BRAIN (BR) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT PITUITARY (PI) : PITUITARY (PI) : NECROPSY: NOT APPLICABLE -MASS(ES); 11 X 9 X 9 MM, RED, TAN, -HEMORRHAGE,-SLIGHT, MULTI-FOCAL >BEHAVIOR; ATAXIC; VERIFIED AT GRAY AND SOFT -B-ADENOMA,-NEOPLASM PRESENT NECROPSY: NOT APPLICABLE OVARY (OV) : OVARY (OV) : >APPEARANCE; HUNCHED; VERIFIED AT -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT NECROPSY: NOT APPLICABLE FILLED CYST, 6 MM IN DIAMETER DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HIND FOOT: -EDEMA,-MODERATE, MULTI-FOCAL SINGLE RED, TAN AND DRY AREA, 5 X 4 -EROSION,-MODERATE, MULTI-FOCAL MM; COLLECTED 4835 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 667 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92460 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/13/00 STUDY DAY OF DEATH: 679 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 289.2 GRAMS DATE AND TIME OF NECROPSY: 02/13/00 8:33 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ FOOT/FOOTPAD (FP) : -INFLAMMATION, ACUTE,-MODERATE, MULTI- FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 4836 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 668 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92461 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/04/99 STUDY DAY OF DEATH: 425 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 371.4 GRAMS DATE AND TIME OF NECROPSY: 06/03/99 7:57 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: NOT APPLICABLE -LARGE PELVIS(ES); RIGHT -DILATATION, PELVIC,-PRESENT >DISCHARGE; NASAL; RED IN COLOR; -GRANULAR MATERIAL; BILATERAL, WITHIN -MINERALIZATION, PELVIC,-MODERATE, VERIFIED AT NECROPSY: OBSERVED PELVES: TAN GRANULAR MATERIAL; MULTI-FOCAL >EYES; RED DISCHARGE; EYES; VERIFIED AT COLLECTED INTACT NECROPSY: OBSERVED LIVER (LI) : >SKIN & PELAGE; PALE; ENTIRE BODY; -VACUOLATION, HEPATOCELLULAR,-MINIMAL, VERIFIED AT NECROPSY: NOT APPLICABLE MULTI-FOCAL >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED PANCREAS (PA) : AT NECROPSY: NOT APPLICABLE -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : NECROPSY: NOT APPLICABLE -CYST(S); ALL REGIONS, DIFFUSE: -GALACTOCELE,-MINIMAL, MULTI-FOCAL MULTIPLE TAN AND CREAMY FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, RED, DARK -CYST,-PRESENT RED, TAN AND SOFT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 4837 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 669 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92461 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/04/99 STUDY DAY OF DEATH: 425 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 371.4 GRAMS DATE AND TIME OF NECROPSY: 06/03/99 7:57 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED -DISCHARGE-NASAL; BILATERAL: RED -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 4838 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 670 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92462 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 687 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 279.2 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 13:21 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -CAST, PROTEINACEOUS,-MINIMAL, MULTI- >EXCRETION; FEW FECES; VERIFIED AT FOCAL NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-SLIGHT, MULTI- >EYES; RED DISCHARGE; EYES; VERIFIED AT FOCAL NECROPSY: OBSERVED THYROID (TY) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -CYST, ULTIMOBRANCHIAL,-PRESENT NECROPSY: NOT APPLICABLE -HYPERPLASIA, C-CELL,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -GALACTOCELE,-SLIGHT, MULTI-FOCAL AND FIRM (CAP 18) -HYPERPLASIA, GLANDULAR,-MODERATELY SEVERE, DIFFUSE BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATE, FOCAL -PIGMENT,-SLIGHT, FOCAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 8 X 8 MM, DARK RED, -HEMORRHAGE,-MODERATE, MULTI-FOCAL GRAY, AND SOFT -B-ADENOMA,-NEOPLASM PRESENT VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; LIGHT GREEN AND >UNREMARKABLE MUCOID DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4839 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 671 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92462 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 687 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 279.2 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 13:21 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND CRUSTED -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA) ------------------------------------------------------------------------------------------------------------------------------------ 4840 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 672 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92463 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 238.0 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:26 PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.5785 3.1842 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : UNVERIFIED AT NECROPSY -NO MACROSCOPIC LESIONS -------------------------------------------- 4841 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 673 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92463 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 238.0 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:26 PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) ------------------------------------------------------------------------------------------------------------------------------------ 4842 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 674 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92464 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 240.5 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:37 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.7170 3.2087 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : VERIFIED AT NECROPSY: OBSERVED -NO MACROSCOPIC LESIONS -------------------------------------------- 4843 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 675 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92464 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 240.5 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:37 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) ------------------------------------------------------------------------------------------------------------------------------------ 4844 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 676 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92465 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 287.6 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:23 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.6100 2.6460 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : VERIFIED AT NECROPSY: OBSERVED -NO MACROSCOPIC LESIONS -------------------------------------------- 4845 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 677 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92465 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 287.6 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:23 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) ------------------------------------------------------------------------------------------------------------------------------------ 4846 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 678 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92466 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 267.4 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:23 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.5092 2.8082 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : VERIFIED AT NECROPSY: OBSERVED -NO MACROSCOPIC LESIONS -------------------------------------------- 4847 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 679 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92466 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 267.4 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:23 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) ------------------------------------------------------------------------------------------------------------------------------------ 4848 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 680 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92467 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 205.2 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:33 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 8.2470 4.0190 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : UNVERIFIED AT NECROPSY -NO MACROSCOPIC LESIONS -------------------------------------------- 4849 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 681 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92467 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 205.2 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:33 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) ------------------------------------------------------------------------------------------------------------------------------------ 4850 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 682 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92468 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 249.4 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:01 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.7884 3.1229 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : VERIFIED AT NECROPSY: OBSERVED -NO MACROSCOPIC LESIONS -------------------------------------------- 4851 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 683 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92468 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 249.4 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:01 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) ------------------------------------------------------------------------------------------------------------------------------------ 4852 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 684 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92469 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 229.4 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:42 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.3542 3.2058 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : VERIFIED AT NECROPSY: OBSERVED -NO MACROSCOPIC LESIONS -------------------------------------------- 4853 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 685 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92469 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 229.4 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:42 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) ------------------------------------------------------------------------------------------------------------------------------------ 4854 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 686 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92470 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 204.6 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:24 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LEFT ADRENAL (AD0) -------- -------- -------- NOT TAKEN RIGHT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LEFT KIDNEY (KD0) -------- -------- -------- NOT TAKEN RIGHT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.3676 3.6010 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LEFT OVARY (OV0) -------- -------- -------- NOT TAKEN RIGHT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; GENERAL COMMENT (GC) : UNVERIFIED AT NECROPSY -NO MACROSCOPIC LESIONS -------------------------------------------- 4855 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 PAGE: 687 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92470 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 204.6 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:24 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) ------------------------------------------------------------------------------------------------------------------------------------ 4856 APPENDIX 7 Cell Proliferation Report Covance 6329-212 Covance 6329-228 3M T-6316.1 4857 Covance 6329-212 Covance 6329-228 3M T-6316.1 4858 Covance 6329-212 Covance 6329-228 3M T-6316.1 4859 Covance 6329-212 Covance 6329-228 3M T-6316.1 4860 Covance 6329-212 Covance 6329-228 3M T-6316.1 4861 Covance 6329-212 Covance 6329-228 3M T-6316.1 4862 Covance 6329-212 Covance 6329-228 3M T-6316.1 4863 Covance 6329-212 Covance 6329-228 3M T-6316.1 4864 Covance 6329-212 Covance 6329-228 3M T-6316.1 4865 Covance 6329-212 Covance 6329-228 3M T-6316.1 4866 Covance 6329-212 Covance 6329-228 3M T-6316.1 4867 Covance 6329-212 Covance 6329-228 3M T-6316.1 4868 Covance 6329-212 Covance 6329-228 3M T-6316.1 4869 Covance 6329-212 Covance 6329-228 3M T-6316.1 4870 Covance 6329-212 Covance 6329-228 3M T-6316.1 4871 Covance 6329-212 Covance 6329-228 3M T-6316.1 4872 Covance 6329-212 Covance 6329-228 3M T-6316.1 4873