Document VJGo6XEQMmQbkgZpOg4ej6mp
BIOCONCENTRATION
TEST SUBSTANCE
Identity: N-ethylperfluorooctane sulfonamidoethanol; may also be referred to as N1:-1E,2{,F2O,3S.E3.A4l,4c,o5h,o5l,6o,r6,F7M.7-,384,2828.-he(p1t-aOdcetcaanfelsuuolrfoo-nNa-m(i2d-ehy,drN-oextyheytlh-yl)-, CAS #1691-99-2)
Remarks: Material is an off-white, waxy solidof uncharacterized purity.
METHOD:
fMoertdheotde/rgmuiindienlgitnhee faoqlulaotwicedB:io3cMondceernitvreadtimoentFhaocdtoorutolfiNne-dEitnFtOhSeEteasltcophroolc.edure
Type: Static GLP (YN):
exposure No
with
a
flow-through
clearance
phase.
Year: 1978
Species: Channel catfish (Ictalurus punctatus)
Supplier: Not noted in the report.
Analytical monitoring: Concentration GC with electron capture; temperature
of
N-EtFOSE
alcohol
in
water
and
fish
by
Length and weight: Not noted in the report.
Exposure period: 7 days
Clearance period: 5 days
Statistical methods: 3M Test fish age: Not noted
Trac system, in report.
MINITAB
II
program
Loading: Not noted in report
Pretreatment: Not noted.
Test conditions:
Dilution water: Carbon-fitered well water
Dilution water chemistry: Not included in report.
Sprteopcakreadndbytedsitsssoollvuitnigo5n7pgrrepaamrsatoifoNn-:ET{hFeOSprEimalacroyhsotloicnk5s0olmultioofn was
eacveatpoonrea,tianpgpolfyfitnhgethaecestoolnuet,ioanntdo c1i0rcpuolautnidngscoafr3b.o5nmfimltegrleadswselbleawdast,er
ltohardoiunggh rtahteiogolfas0s.5begardasm.s Tpehrelsityers.teAmerwoabiscdceosnidginteidontsowreersueltmaininatafiinnaeld
throughout and the system was assumed time allowed to circulate was not noted.
to
go to
saturation.
Length of
Exposure vessels: 114 liter aquarium. Number of replicates: Two
Volume utilized was
not
noted.
Numberoffish per replicate: Not noted in the report.
Number of concentrations: Water chemistry during the
Osntuedyp:lus
negative
control
Dissolved oxygen: Not noted in the report.
006242
TpeHs:t Ntoetmpneortaetdurine:th2e 1re1porCt. Photoperiod: 16 hours light and 8 hours dark with a 30 minute transition period
Remarks field: Following the introduction of the channel catfish into the
sexapmeprliemse,nttoapl, amnidddcloentarnold abqoutatroima lsaaymerp,lfeosr oN-f4EtcFhaOnSnEelalccaothfoilshuapntdaktehraenealwyastiesr
wmienrueteosb,taoinneedhoautrt,htewfoolhloouwrisn,g ftoiumrehpoeurriso,dse:igChotnhtoruorls,,ptriwoerlvtoe ihnoturrosd,ucstiixotne,e1n5
pheoruriso,dotfwe7ndtayysh.ourTs,hetwcehnatnyn-efolucrathfoiusrhs,wearned tahtetnwernetmyo-vfoeudr thooculreianrtaenrcvaeltsafnokasrfor
tthwroohuoguhrtsheantdestficnahlalymbtrearnssofferfroeudrtowaatfelrovwo-tlhurmoeusghpesrys2t4emhohuarsv.inAg
flow rates sampling
regimen initiated
aindednttiecramlitnoattheadtaofftetrhefivueptdaakyes.phaseofthe
study
was
immediately
RESULTS
--ee
N-EtFOSE Alcohol Uptake by Channel Catfish (Ictalarus punctatus) -- Whole fish samples.
[om [TM&r =a ow
o
sm
[--fhhewws 7T79 a16|
[how ehos----15 77776 1 2&0 ||
hes ew ___|
[[foeohhoowwssT6S 1 | T sm s ]
athows
dor[0]
[ashes
er
ars
[ [aBIhN hoo1 wws sS T I i oiSE | g E a2o erE r ||
[Csehshoowwss| smr0 | | 1o8p |
006243
NEIFOSE Alcohol Clearance of Channel Catfish (ctalarus punctatus) - Whole fs samples
[=[Eeego | ESgigT
[smtn hw [ a036 320m6 || [showhosws [ [0 0[2 2 m] ] [ I ha7 sSO[ IS a E s tr] [[hagobw owss [ [2 2 | | 2 2051]] [[drghhoows [at 0 wzo] r | CmhSohwowss [we qs[ | a 7 |
Sample lost N-EIFOSE Alcohol Uptake of Channel Catfish (ictalarus punctatus) Muscle
a (iglg
[ [fh omw n_[ --T3 ar | [ [hhouws s 59] 0] [ [e zr ho os s[ [= 5m 1 || [h[ ow fse| h | o3ws|
[ [h ho ow w w6e1 s s|| [r Y hsT S r
[foh | otwss ]
006244
N-EFOSE Alcohol Clearance of Channel Catfish (italarus punctatus) Muscle
[me gig
[smn Thou Tr
[hows 108
[ [h B oh ws s Tq 47] [--T--o 717hoqaw g s|
[--[ To2 hou0 sT hoa u rr ws| [ [d 2 8h --h | 1o ou w 7q05r 2 s s||
[Shows 1760
|
[h--o 77 w 2 s
cNt-aElWaFrOuSsEpuAnlcctoahtoulsU)ptake by Brain Tissue and Gastrointestinal Tract of Channel Catfish
Time,
Brain Concentration | Gastrointestinal Tract
(uglo)
Conceungtgration
Gsemmwnol7 ----o--e01|
f --f--hhewwsTT Rep|
[hous
[Shows
7150
ai
|
00s]
[f[--eghhoouuws s --T | dae s | i0 io ||
[20houdwhssT 00 s a5006r ||
[--d8houws [--7zhows
T Ta s ss e a0o||
Sh12o0 hwo[ usrs ss 06 T| se ss
| |
[----Tdhows CC fBhows
--16 | 0e5 ss |
|
05
|
535
|
006245
N-EIFOSE Alcohol Clearance of Brain Tissue and Gastrointestinal Tract of Channel Catfish
(lctalarus punctatus)
Tire
Brain Concentration | Gasteiesinal Track
(uglg)
`Concentration
Cem new ers
w 7T e s r ] ----|
7p
ms
hhos ws hows
Ts er ip sr sp ----]
[ohetohhoos[ Ts ss eT s t s s ze s----
[rhohos ons--
[ [ asm a sT sa 5s 0 --] --
Crows|r 7m ----]
pNu-nEcItaFtOuSsE) Alcohol Uptake by Liver, Kidney, and Gall Bladder of Channel Catfish (ictalarus
Time
TerConcantation | Kidney Concentration|
(ug/g)
(ug/g)
hhmowwns [ [na n wr pr aqrr
[zhhh oowss a r r o s y------]
oehhhooos[ T T os rw To g ag----
Cp Cs
moer
gnows -- [der
p0g] 3%
Cohsoo hhoewss[ s a or s t35p
Gall iadder 0pe1 Concentration
oEXt} 0<o11 214558 227312 71538853 2707735 T33286015
006246
N-EIFOSE Alohal ClearanceofLive, Kidney, and Gall BlaodfCdhaennrel Catfish (calarus
punctatus)
wrCancontraion | Kidney Concentration |
(uglg)
(ug/g)
[ [s hm or nT s e m ser
[[hho ow ws s Tm 7
[shows
sor
a amsor]
[[heohwos s1 T 7a m
mms
0
| s swiso s] ]
[[hoohros Te as s|ta] s]
[ [sreh hossT [7s % [om fo ]
Cmhors Ts
as]
Gall Biaddar Conceg1nit5ration
520858 53075180 aa24r975 S2081120 2Ta2t11 BST
CONCLUSIONS
--
----
No reliable conclusions can be derived from this study. General observations
can be made.
eNx-pEotsFurOeS.E alcohol concentrations increased in the fish tissues over the course of
Lipophilic organs and organs possessing a large surface area at the water/organ
interface possess the highest uptake rate constants.
The brain and gastrointestinal tract have potentially high bioconcentration factors due to the lipophilic natureof N-EtFOSE alcohol.
The muscle fillet appeared to exhibit the least potential to bioaccumulate NEtFOSE alcohol.
The gall bladder showed the highest concentration of N-EtFOSE alcohol
There is a potential for excretion of N-EtFOSE alcohol through both urinary and
fecal pathways.
No signs of toxicity were apparent at the exposure concentration.
While concentration decreased in the whole fish samples at the end of the clearance phase, the time required for complete removal is longer than the 5
days used in the test.
006247
Submitter: Minnesota,
3M Company, 55133
Environmental
Laboratory,
P.O.
Box
33331,
St.
Paul,
DATA QUALITY
Reliability: Klimisch ranking 3. There is a lackof description for the study's methodology and characterization of the test organisms. There is no attempt at correlating, nor is there any record of, the size, age, and body fat contentof the fsiasmhpalessi.t reTlhaitsesistoestpheecicaolnlcyenitmrpaotritoanntofduNe-EttoFtOheSEfacatlctohhaotlolfdoeurn,dlainrgtehrefvisahriaorues likely to have more fat content than younger, smaller ones. The vigorous method used to solubilize the test substance appears to have resulted in a. supersaturated solution. The solubility is characterized as being 50 ppb, but the concentration in the aquarium was given to be 500 ppb, andit was noted that the N-E{FOSE alcohol was present as a suspension. Moreover, it was also noted that excitability of the test organisms would cause disturbance of the bottom layer and re-suspension of N-EtFOSE alcohol into the water. Test substance purity was not characterized.
Third party review by Dr. James W. Gillett of Comell University (observations included with report) also indicate a lack of reliability.
REFERENCES
3M Technical Report "Evaluation of the Bioconcentration Potential of FM 3422" A. N. Welter, Project 9970612623, Fate of Fluorochemicals, Report Number 2, August 16, 1978
3M requested expert overview, "Bioaccumulation Studies", Dr. James Gillett, Comell University, March 8, 1993
OTHER
Last changed: 5/18/00
006248