Document VGok88B6LykgZrO75BRBK64Yw

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredto as PFOS, FC-95, or as a component of FC-603F. (1Octanesulfonicacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 501. The testsample isFC603F. Currentinformationindicatesitisa mixtureof 0.5% PFOS, 7% diethyleneglycolbutylether,82.95% water,2% acrylifcoamer,4% Alkylether sulfate1,.45% Xanthan gum, 1.4% calciumacetate,0.07% N-(3Chloroallyl)hexaminiucmhloride0,.5% tdethanolamine,0.05% sodium hydroxide,0.03% cis-1-(3-Chloroallyl)-3,5,7-td-aazzao-n1iaadamantane chloridea,nd 0.05% tolytriazole. The followingsummary applies toa mixture with incompletely characterizeccfoncentrations of impurities.Data may not accurately reflectthe toxicityof the fluorochemical component of the testsample. METHOD: Method: OECD Guideline203. Type: Acute static GLP: No Year completed: 1994 Species: Pimephalespromelas Supplier:AquaticBiosystems,Inc.F,ortCollinsC,O. Analyticalmonitoring: Temperature,pH, and dissolvedoxygen. Exposure period: 96-hours Statisticamlethods: LC50valueswere calculatebdy theTrimmed Spearman-Karber method. Test fishage: Juveniles(53 days) Length and weight: Average length 16 mm Average weight 0.0276 g (wet) Loading: 0.276 g/L Pretreatment: None. Test conditions: Dilutionwater: Carbon-filterewdellwater (3M Well #2, St.Paul,MN) Dilutionwater chemistry: Not given. Lighting: Not given. Stock and testsolutionpreparation: The testmaterialwas added tothedilutiownateron a weighttvolumebasis. Concentrations dosing rate:Once. Stabilitoyf the testchemical solutions: Not noted. Exposure vessels: 1 L vesselscontaining1 L testsolution. Number of replicates:2. directly Number offishperreplicate1:0. Number ofconcentrationsf:iveplusa blankcontrol Waterchemistryduringthestudy: pH range(0-96hours): 8.0- 8.3(contreoxlposure) 8.0- 8.3(560 mg/L exposure)* Temperature range (0-96hours): 19.5- 20.5OC Dissolved oxygen range (0-96hours): 6.8 - 8.5mg/L (controelxposure) 3.6- 8.5 mg/L (560 mg/L exposure)* 560 mg/L (second highestconcentratione)xposure values used because the highestconcentrationused resultedintotalmortalitbyy 24 hours. RESULTS Nominal concentrations: Blank control,100, 180, 320, 560, and 1000 mg/L Element values: 96-hour LC5o = 750 mg/L (Clnot calculable) Element values based on nominal concentrations Remarks: Testingwas conducted on a mixtureas describedintheTest Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The FC-603F 96-hour LC50 forfatheadminnow was determined tobe 750 mg/L. Subminer: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 2. This studymeets the criterifaorquality testing.However, sample puritywas notproperiycharacterizedand thestudy lacksanalyticaclonfirmationofthe amount offluorochemicalproportioninthe solution. REFERENCES This studywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number Ml 655, 1994. OTHER Last changed: 6/26/00 ENVIRONMEr4TAL LABORATORY LFL-M1655 Date: 5/27/94 Analyst:JBD Pimephales pron?etas 9rv-hourToxicity Test Data Summary SAMPLE: FC-603F (Lot#501) Sample Conc. (mg/L) 24 hours Percent Monality 48 hours 72 hours 100 0 0 0 180 0 0 0 320 0 0 0 560 0 0 0 1000 100 100 100 LCSO Estimation Method: Trimmed Spearman-Karber 24 h LC50 (95% C.I.)= 48 h LC50 (95% C.I.)= 72 h LC50 (95% C.I.)= 96 h LCSO (95% C.I.)= 750 mgtl (Not Calculable) 750 mgtl (Not Calculable) 750 mg/L (Not Calculable) 750 mgtl (Not Calculable) 96 hours 0 0 0 0 100 ENVIRONMENTAL LABORATORY 96-HRACU rE TOXICITYTO FATHM MINNOW. PIMEPHALES PROMELAS TYPE TEST-. STATIC STATIC RENEWAL TEST SUBSTANCE.DILUTION WATER: TEST FISH SOURCE: FISH AGE: (Ic ( L (.t CARBON-RLTERED WELL WATER (3M WELL #Z ST. PAUI- MN) TEMP. 46 IC.pH 'S D.O. Vmg(L C - @ k, C-) DATE RECEIVED. %-\- --4 "G. WT-* r,,c.r rg "r.. SIZE. NO. OF FISWREST CHAMBER: LOADING RATE. TEST SOLUTION VOLUME. DATE INITIATED: k 4 7@@- SIZE OF TEST VESSELS: DATE TERMINATED-. LR (V\(c -(%\,\ TEST INITIAL TOT MORTALITY VESSE CONC. AMOUNI 24-HR pH D.O. 48-HR pH 0.0. 72-HR pH mgAL ADDED* I i Mg/L mgAL BKI CONTROL C! I -I.-; C-. I SK2 CONTROL -x ci I 0.0. 96-HR pH 0.0. MgA. mg/L Cv.t% C. c, -CA 2 C-. c (L @-I (L Ct I c 2 LS IL c 2 C C @c. C, 2 1 1. C, 2 IC 'A -A c\ r 2 2 2 TEMP. *C IDATE / M&YST LCSO (gs%.C.I.) COMMENTS -1-Tc, k @t- @3%w@4k"5' L Cts- C% ne,@- N.),e- SECONDARY EFFECTS: s s6 bag ANALYST. e-iL6oss otEqudibnum;a OW*scont R ErraM6Swmmng; H Hmnorrh&WV @.'L-TA IF, Ef LIE